LOCUS       DASYJA010000001         4359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2724096, whole genome shotgun sequence.
ACCESSION   DASYJA010000001 DASYJA010000000
VERSION     DASYJA010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2724096"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VGO02_00005"
     CDS             <1..692
                     /locus_tag="VGO02_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HEV7476098.1"
                     /translation="EVKVKRRLRVAFFATGDELASIGDKLKAGEVYDSNRYTLHGMLS
                     RLGVVRDDPKALEAALHKAAKADAIITTGGVSVGEADFIRELMAKLGEVLFWKIAMRP
                     GRPMAFGRIGNAFLFGLPGNPVAVMVTFYEFVRDALLYLSGRTDDFAIPLLRVISSEK
                     IRKVPGRTEYQRGILYQEQNEWRVRTTGQQGSGVLRSMAEANCFIVLEHERGAVTAGE
                     QVAVQLMEAIA"
     gene            689..1162
                     /locus_tag="VGO02_00010"
     CDS             689..1162
                     /locus_tag="VGO02_00010"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEV7476099.1"
                     /translation="MSPIHPSLALLAGAAKQRNWAGLQKQLGLALRKPAWLRPEHRVG
                     DPTGYMRHLLYIDPHGDFVMTAITWLPGQVSARHGHQVWCAFGVAEGELTEEQFDAKL
                     LVSKSSVLRPGDIADRDLEGTIIHQVANRGTAPLVSLHLYGVSADRLTTGINRYF"
     gene            complement(1164..2135)
                     /locus_tag="VGO02_00015"
     CDS             complement(1164..2135)
                     /locus_tag="VGO02_00015"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEV7476100.1"
                     /translation="MKKIAVLTSGGDAPGMNAAVRAVTRGALAKGWEVFGVRNGYAGL
                     LAGTIEPLAARNVGGIVQQGGTVLGSARCPEFIEAAGRKQALSNLNKLGIEALVVVGG
                     NGSQTGSASLAREGYPVVGVPSTIDNDLFGTDVSIGADTAINVTLEAIDRLRTTASSH
                     QRAFAVETMGRDCGYIALMSGIAGGAEVIALPESEIRPVDVAERLRDAYRRGKTHALA
                     VIAEGAKCGVHEIMEYYAAHRESIGYELRVTRLGHVVRGGTPGAADRVLATRLGAAAV
                     EALAAGRHGVLVGIVRNAIANTPLAEIAGRTRPVDTALLELARTMAM"
     gene            2156..2836
                     /locus_tag="VGO02_00020"
     CDS             2156..2836
                     /locus_tag="VGO02_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747910.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEV7476101.1"
                     /translation="MSEALKMLREDARGCRACPLWKNATQTVFGAGSPHARVMLVGEQ
                     PGDEEDRRGKPFVGPAGRLLDRALEAAGVDRGTLYVTNAVKHFKWQPRGKRRLHKTPA
                     QREIEACRPWLEGEIAAIRPEVICCLGATAAKALLGRRFKVSLSRGKFIDSPYAQRVF
                     ATLHPSAILRVRGAAEREAAFRSLVRDLALMRSTAPATPALPPDRAGSSARRSAPRRP
                     STSARRGG"
     gene            complement(2802..3443)
                     /gene="otsB"
                     /locus_tag="VGO02_00025"
                     /pseudo
     CDS             complement(2802..3443)
                     /gene="otsB"
                     /locus_tag="VGO02_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
     gene            complement(3445..>4359)
                     /locus_tag="VGO02_00030"
     CDS             complement(3445..>4359)
                     /locus_tag="VGO02_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEV7476102.1"
                     /translation="GLLGSSILGFHTQFHCNNFFDTVDRFLEARVDRETFSIAHGGET
                     TEVRRYPISIEWPPAALQVQPPVAECRQRVRESLNVGQQIKLGVGVDRLDYTKGILER
                     FMAIERLLELEPHWIGKFSFLQVAAPSRSSIDEYQNLDARVRALAERINARFGTATYQ
                     PIILKIEHHDAAQVYELYRASELCYVSSLHDGMNLVAKEYVAARDDEQGVLILSQFTG
                     AARELAEALIVNPYDIEQSAAAIHLALTMSPDEQRARMRSMRNLVQEFNVYRWAGRML
                     LDAARMRHRRRVLAPSRRAAARRQAGGG"
BASE COUNT          667 a         1524 c         1510 g          658 t
ORIGIN      
        1 ccgaggtcaa agtcaaaaga aggctgcggg tagcattctt cgccaccggc gacgagctcg
       61 cctcgatcgg cgacaagctg aaggcgggcg aggtctacga ctcgaaccgc tatacgctgc
      121 acggcatgct gtcgcgcctc ggcgtggtgc gcgacgatcc gaaggcgctc gaagccgcgc
      181 ttcacaaagc ggcgaaggcg gacgccatca ttaccaccgg cggcgtgtcg gtcggcgagg
      241 ccgatttcat ccgcgagctg atggcgaagc tgggggaagt gctcttctgg aagatcgcca
      301 tgcgcccggg ccggccgatg gcgttcggca ggatcggcaa cgccttcctg ttcggcctgc
      361 ccggcaaccc ggtggcggtg atggtgacgt tctacgagtt cgtgcgcgac gcgctgctct
      421 atctatcggg acggacggat gatttcgcca ttccgctgtt acgggttatc tcttcagaaa
      481 aaatcaggaa ggtccccggc cgcaccgagt atcaaagggg aatcctctac caggagcaga
      541 acgaatggcg cgtgcgcacc accggccagc agggctcggg cgtgctgcgc tcgatggccg
      601 aggcgaactg cttcatcgtg ctggagcacg agcgtggcgc ggtcaccgcc ggcgagcagg
      661 tcgcggtgca gctgatggag gcgatcgcat gagcccgatc cacccgagcc ttgcgctcct
      721 cgccggcgcg gcgaagcaga ggaactgggc cggcctgcag aagcagctcg gcctggccct
      781 gcgcaaaccc gcctggcttc gccccgagca ccgcgtcggc gacccgaccg gctacatgcg
      841 ccacctgctg tacattgacc cgcacggcga cttcgtcatg acggcgatca cctggctgcc
      901 cgggcaggtc agcgcgcgcc acgggcatca ggtgtggtgc gcgttcggcg tggcggaagg
      961 cgagctgacc gaggagcagt tcgacgcaaa actgcttgtt tcaaagagca gcgtcctgcg
     1021 gccaggcgac atcgccgacc gcgacctcga gggaaccatc attcaccagg tggcgaaccg
     1081 gggaaccgcg ccgctcgtct cgctgcacct gtacggcgtg tcagccgaca ggctgacgac
     1141 cggcatcaac cggtacttct agctcacatc gccatggtcc gcgcgagctc gagcagcgcg
     1201 gtgtcgaccg gccgcgtgcg gccggcgatc tcggcgagcg gcgtgttcgc gatcgcgttg
     1261 cgcacgatgc cgaccagcac gccgtgccgg ccggcggcga gcgcctcgac cgccgccgcg
     1321 cccaggcgcg tggcgaggac gcggtcggcc gcgccgggcg tgccgccgcg cacgacgtgg
     1381 cccaggcgcg tcacgcgcag ctcgtagccg atcgactcgc ggtgcgccgc gtagtactcc
     1441 atgatctcgt gcacgccgca tttcgcgccc tcggcgatca cggcgagggc gtgcgtcttg
     1501 ccgcggcggt aggcgtcgcg cagccgctcc gccacgtcga ccggccggat ctcgctctcg
     1561 ggcagggcga tcacctcggc gccgccggcg atgcccgaca tcagcgcgat gtagccgcag
     1621 tcgcgaccca tggtctcgac ggcgaaggcg cgctggtgcg aggaagcggt ggtgcgcagg
     1681 cggtcgatcg cctcgagggt gacgttgatg gcggtgtcgg cgccgatgct gacgtcggtg
     1741 ccgaagaggt cgttgtcgat ggtcgagggc acgccgacca ccgggtagcc ttcgcgcgcc
     1801 aggctcgccg agccggtctg cgagccgttg ccgccgacga ccacgagcgc ctctattccc
     1861 agcttattca aattcgaaag agcctgcttg cgcccggcgg cctcgatgaa ctccgggcag
     1921 cgcgcgctgc cgagcaccgt gccgccctgc tgcacgatgc cgccgacgtt gcgcgcggcg
     1981 agcggctcga tggtgccggc gagcaggcca gcgtagccgt tgcggacgcc gaagacttcc
     2041 cagcccttgg cgagcgcgcc gcgcgtcacc gcgcgcaccg cggcgttcat gccgggcgcg
     2101 tcgccgccgc tggtgaggac tgcaatcttt ttcaagatcc cgattctaac gacccatgag
     2161 cgaagccctg aagatgctgc gcgaggacgc ccgcggctgc cgcgcctgcc cgctgtggaa
     2221 gaacgcgacg cagacggtct tcggcgccgg ctcgccccac gccagggtca tgctggtcgg
     2281 cgagcagccc ggcgacgagg aggaccggcg cggcaagcct ttcgtcggtc cggccggacg
     2341 cctgctcgac cgcgcgctcg aggcggccgg cgtcgaccgc ggcaccctgt acgtcaccaa
     2401 cgcggtgaag catttcaagt ggcagccgcg cggcaagcgt cgcctgcaca agacacctgc
     2461 gcagcgcgag atcgaggcct gccgtccctg gctagagggc gagatcgccg caatccggcc
     2521 cgaggtgatc tgctgcctcg gcgccacggc cgccaaagcg ctgctcggcc ggcggttcaa
     2581 ggtatctctc tcaagaggta aattcatcga cagcccgtac gcgcagcgcg tcttcgccac
     2641 cctccatccg tcggcgatcc tgcgcgtgcg cggcgcggcc gagcgcgagg cggccttcag
     2701 gtcgctggta cgcgatctgg cgctgatgcg atccactgcg ccagccacgc ccgcgctgcc
     2761 gccggatcgg gcaggctcca gcgcgcggcg gtcggccccg cgccgacctt caacgagtgc
     2821 ccgccgaggc ggttgacgag cgtgaacccg tgctcgtcgg atacgtcgtc gccgaggaac
     2881 accggcacgc ggcgcgcgaa cggccgctcg tgcatgaact cctcgattgc gacgcccttg
     2941 tcgcggccgc tcggccgcag ctcgaacacc atcttgcccg gcaggacctc gaagcgctcg
     3001 cccaggcgcg ccgccgattc ctcgatcgcg gccttcaccc ccttctccag gcccggcgca
     3061 aggcgatagt gcagcgcgac gctcagcccc ttgtcctcga agatcaggcc ctcgtgcagc
     3121 gcggcgtagc gccgcagcgg cgcgatcgcg ctgcgccaca gctcgctcgg cggagcattg
     3181 cgcaggatcc tgccgcgcgc gtcgcgccgc tcgacgccgt gctgcccggc cgccggcagc
     3241 ttgagcggcg cgaacatctt gtcgatgccg gcgatcggcc ggccgctcac cagcgccacc
     3301 gcgccgcccg ccgcctcgta cagcgcagtc aatagctcga agtcggcggc cccggcgtgc
     3361 accgccgccg gcagcgcgac gatgtcgaac agcgtgccgt cgatatcgag gaagaatgcc
     3421 cagttggcag cgaagggcgg catgctagcc cccgcccgcc tggcgccggg ccgcggcccg
     3481 gcgcgagggc gccaggacgc ggcggcggtg gcgcatgcgc gccgcgtcga gcagcatgcg
     3541 gcccgcccag cggtacacgt tgaactcctg caccaggttg cgcatgctgc gcatgcgcgc
     3601 gcgctgctcg tccggcgaca tggtgagcgc caggtgtatc gccgcggcgc tctgctcgat
     3661 gtcgtaggga ttgacgatca gggcctcggc cagctcgcgc gccgcgccgg tgaactgcga
     3721 caggatcaat acgccctgct cgtcgtcccg cgcagccacg tattcttttg ccaccaggtt
     3781 catgccgtcg tgcaggctgg agacgtagca cagctcgctg gcgcggtaca gctcgtagac
     3841 ctgcgccgcg tcgtggtgct cgatcttgag aatgatcggc tggtaggtcg cggtgccgaa
     3901 acgcgcgttg atccgctcgg cgagcgcgcg cacgcgggca tcgaggttct ggtattcgtc
     3961 gatcgatgag cgcgacggcg ctgcgacctg caggaacgag aacttgccga tccagtgcgg
     4021 ctcgagctcg agcagccgct cgatcgccat gaagcgctcc aggatcccct tggtgtagtc
     4081 gagccggtcg acgcccacac cgagtttgat ttgttgtcca acatttaaac tctctctcac
     4141 gcgctggcgg cactccgcca ccggcggctg cacctgcagc gcggcgggcg gccattcgat
     4201 ggagatcggg tagcggcgca cctcggtggt ctcgccgccg tgcgcgatcg agaacgtctc
     4261 gcggtcgacc cgcgcctcga ggaagcggtc caccgtgtcg aagaagttgt tgcagtggaa
     4321 ctgcgtgtgg aagccgagga tcgacgagcc gagcaggcc
//
LOCUS       DASYJA010000002         2216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2451896, whole genome shotgun sequence.
ACCESSION   DASYJA010000002 DASYJA010000000
VERSION     DASYJA010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2451896"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VGO02_00035"
     CDS             complement(<1..232)
                     /locus_tag="VGO02_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEV7476103.1"
                     /translation="MLVVVDPEEPTVRVWDPLVRAAHWLLVASVLVAWITIELPGKLA
                     AAVHEWAGYTALGVVALRFVWGLVGPRYARFAQ"
     gene            complement(233..553)
                     /locus_tag="VGO02_00040"
     CDS             complement(233..553)
                     /locus_tag="VGO02_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEV7476104.1"
                     /translation="MEIGMKSQRFRLAAPAAACLCLVLLAAPAARGTGLATCDSGSQE
                     LWQPQETLAARLKEKGWQVRRIKVDGGCYEVYALDENGKRVEAYFHPRTFAPVPVGAK
                     DHKG"
     gene            complement(629..1480)
                     /gene="xerD"
                     /locus_tag="VGO02_00045"
     CDS             complement(629..1480)
                     /gene="xerD"
                     /locus_tag="VGO02_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEV7476105.1"
                     /translation="MSPALLDAFCDALWLEDGLSKNTIASYRADLLQLAGFLHGKLLE
                     AKEEQLFGFLASRKGKASSAARMVSTLKRFYQYCLRERRIPADPTIRLDPPKRAPRFP
                     KSLSEDDVEALLGAPDTASQLGLRDRAMLETLYATGLRVSELVSLKTFEVNLNSGVVR
                     VMGKGSKERLVPLGEEAVDWIAKYMQSRKKASNTLFITARGGGMTRQAFWQLIGRYGR
                     RAVPGKKLSPHVLRHAFATHLINHGADLRVVQMLLGHADISTTQIYTHVARERLKALH
                     AKHHPRG"
     gene            complement(1477..1917)
                     /locus_tag="VGO02_00050"
     CDS             complement(1477..1917)
                     /locus_tag="VGO02_00050"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV7476106.1"
                     /translation="MATRGGKVIGIRYLPLSTENIPSKNPLAERAARQLERYRDDPDT
                     VFDLPLLIEGSPLQRGVWQAMCAIPRGRTRTYGDLARELGADARAIGQACGDNRLPIV
                     IPCHRVVAADGIGGFSHTTEGYLIEVKRWLLAHEIRPEEFALKP"
     gene            complement(1947..>2216)
                     /locus_tag="VGO02_00055"
     CDS             complement(1947..>2216)
                     /locus_tag="VGO02_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEV7476107.1"
                     /translation="AIGPEADSFFAEQGNKPRIGQAFLAIDPAALAGREKYLERVETV
                     VRTMLADEGVRLPGAKRFGSQKRARAEGLEIPDDLLAKIEKLCTT"
BASE COUNT          378 a          734 c          734 g          370 t
ORIGIN      
        1 actgcgcgaa gcgcgcgtag cgcggcccaa ccaggcccca gacgaaccgc agcgcaacga
       61 cgccgagcgc ggtatagccg gcccattcgt ggactgctgc ggcaagcttg ccgggtagtt
      121 caatggtgat ccatgccacc agcacgctag ccaccagcaa ccagtgggcg gcgcgcacca
      181 aagggtccca cacccgaacg gtcggctcct ccggatcgac caccacgagc acctagccct
      241 tgtgatcctt ggcgccaacc ggcaccggcg cgaacgtgcg ggggtggaag tacgcctcga
      301 cgcgtttgcc gttctcgtcc aaggcgtaga cctcatagca tccgccgtct accttgatgc
      361 ggcgcacctg ccatcccttt tccttcaatc gcgctgcgag tgtctcctgt ggctgccaga
      421 gctcctggct gccggaatca caggtggcca agccggttcc ccgcgccgct ggcgccgcga
      481 gcaggacaag gcaaaggcat gccgccgcag gcgcggcgag ccggaagcgt tgcgatttca
      541 tgccgatctc cattgtgcgg tgggccttgt ggcaacgata gccgtgcgca gccaggacga
      601 caacgagatt tgcaatttga aacaaatatc agccacgcgg gtgatgcttg gcgtgcagcg
      661 ccttcagacg ctctcgcgcg acgtgcgtgt agatctgcgt ggtggagata tcggcgtggc
      721 ccagaagcat ctgcaccacg cgcaggtcgg caccgtggtt gatgaggtgc gtggcaaagg
      781 catggcgcag gacgtgcggc gagagcttct ttccggggac agcgcgccgg ccatagcgcc
      841 cgatgagctg ccagaacgcc tggcgcgtca tgccgccgcc gcgggcggtg atgaacaagg
      901 tattcgaagc cttctttctc gattgcatgt acttcgcgat ccagtcgacg gcttcctcgc
      961 ccagcggcac caggcgctcc ttcgagccct tgcccatgac gcgcaccacg cccgaattca
     1021 ggttcacctc gaaagtcttc agcgagacca gctccgagac gcgcaggccc gtcgcgtaca
     1081 gcgtttccag catcgcgcga tcgcgcaggc cgagctggct cgccgtgtcc ggcgcgccga
     1141 gcagcgcctc gacgtcgtcc tccgacaggc ttttcggaaa gcgcggcgca cgcttcggtg
     1201 gatcgagcct gatcgtcgga tcggccggga tgcgccgctc gcgcaggcag tactggtaga
     1261 agcgcttgag cgtggacacc atgcgcgcgg cgctcgacgc cttgcccttg cgggaggcga
     1321 ggaagccgaa cagctgctct tcttttgctt cgagaagttt tccgtgaaga aaacccgcga
     1381 gctgcagcag atcagcgcgg tacgaagcga tcgtgttctt cgacagcccg tcctcgagcc
     1441 agagcgcgtc gcagaacgcg tccagcagcg cggggctcag ggcttgagcg cgaactcttc
     1501 cggacgaatt tcgtgggcga gcagccaacg cttgacctcg atcagataac cttcggtcgt
     1561 gtgcgaaaag ccgccgatgc cgtccgcggc gacgacgcgg tggcacggaa tcacgatcgg
     1621 caggcggtta tcgccgcagg cctgcccgat ggcacgcgcg tcggcgccga gctcgcgcgc
     1681 caggtcgccg taggtgcggg tgcggccgcg cgggatcgcg cacatcgcct gccagacgcc
     1741 gcgctgcagt ggcgagcctt cgatcagcag gggcagatcg aaaaccgtgt cgggatcgtc
     1801 acgatagcgc tccagctgcc gcgccgcgcg ctccgccaga ggattttttg acgggatatt
     1861 ttcagtcgaa agcggcaggt agcggatgcc gatcaccttg ccgccgcgcg tcgccaccgc
     1921 gaccttcatc ctcgggaagt cgacggttac gtcgtacaca gcttctcgat cttggccagc
     1981 aggtcgtcgg ggatctcgag gccttcggcg cgcgcgcgct tctgcgagcc gaagcgcttc
     2041 gcgccgggaa ggcgcacgcc ctcgtcggcg agcatggtgc gcaccaccgt ctcgacgcgc
     2101 tcgaggtact tctcgcggcc ggcgagcgcc gcgggatcga tggcgaggaa cgcctggccg
     2161 atgcgcggct tgttgccctg ctcggcgaag aaggagtcgg cctcggggcc gatcgc
//
LOCUS       DASYJA010000003         3500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2996688, whole genome shotgun sequence.
ACCESSION   DASYJA010000003 DASYJA010000000
VERSION     DASYJA010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2996688"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /locus_tag="VGO02_00060"
     CDS             complement(<1..137)
                     /locus_tag="VGO02_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7476108.1"
                     /translation="MEDKLAIIEVVHNWALWRDAGDWERFRTVWHDDGWMTATWFQGP
                     AA"
     gene            complement(159..1115)
                     /locus_tag="VGO02_00065"
     CDS             complement(159..1115)
                     /locus_tag="VGO02_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7476109.1"
                     /translation="MFRIVSAFLLAALSAPSWAQSYPSRAVKIVVPYGVGGSADLYAR
                     YLGAKLSDNLGQPVVIENRPGAGSIVGTDAVAKSDADGYTLLMMSNTHTVNETLIPKK
                     PFDLMRDLAPITGVSYSDLMMVVASDVPASTLKEFIALAKSKPGALNYASSGNGTPYH
                     MAGELFKSMAGVDIVHIPHKGSDQARAAVLGGQVQMMFDAVPTMAASASAGKVKALAT
                     TGAKRSPVTPNVPTVAEAGVPGYESGIWLGLMAPAKTPRPVLERVNAEVNKIINSPET
                     KETWLKQGTVAMGMPIDQFDRFLREEIVKWAKVVKVSGAKAD"
     gene            1146..2054
                     /locus_tag="VGO02_00070"
     CDS             1146..2054
                     /locus_tag="VGO02_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV7476110.1"
                     /translation="MRILVTGAAGYIGSALARFLAARNLQVVATDQSAPAPLIGNIAY
                     PQFARSLITPEVGAVFHLASLVSGGAEQNFELGTKVNLDATRDLLEACRLAGHCPKFV
                     FASSIAVYSGAEVITDETPPRPRLSYGAQKLIGEILIDDYTRRGYVDGRTLRLPTVMV
                     RPQSANTAVSGWASAIIREPLAGRDYVCPVRPETRMACISLGRVVESFFHAFELPGEK
                     LDSQRTILLNGIPVSAQQMRDAALGRGKVRFEPDPAIQKIMDGVPQASASKRAVALGF
                     RHSANIEEIVDEYKKDHPDPALARNG"
     gene            2005..>3500
                     /locus_tag="VGO02_00075"
     CDS             2005..>3500
                     /locus_tag="VGO02_00075"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017685832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV7476111.1"
                     /translation="MNTKKTIPILPSRVTVEGLDRAPHRAFLHALGLSDADLKKPFVG
                     VASTDGRVTPCNALLGEFVREACAAVRLAGGMPFDFASISVADSMSMNHGGMRYSLLS
                     RELIADGVEAVARGHAYDALVTFAGCDKTLPGMMMAMARLDLPSIFVYGGAALPGKWK
                     GRDVTVLDTYEGVGAVLAGKMGESELNELEHICIPTLGSCPGQFTANTMAMVSETLGL
                     ALPGSATMPAVATERSEVARRAGKIVMELLRNGGPLPKDLITKKSLENACAAVAATGG
                     STNAALHIPAIAHEAGIRFTMDDFARVAKRTPLLADMKPGGRFLAKDLHAVGGVYAVL
                     KALLAHGALHGDCMTIFGSTLEDELRERPEADGEVVRKTPISPTGGLVVLKGNLAPQG
                     ALIKVAGLESLVFEGRARVFDSEEACAEVVRKRTYKEGEVLVIRYEGPKGGPGMREML
                     GVTALLYGQGVGEKVALLTDGRFSGATRGMMVGYASPEAAAGGALSIV"
BASE COUNT          572 a         1178 c         1150 g          600 t
ORIGIN      
        1 gccgccggcc cctggaacca ggtggccgtc atccagccgt cgtcgtgcca gaccgtgcgg
       61 aagcgctccc agtcgccggc gtcgcgccag agggcccagt tgtggacgac ttcgataatg
      121 gcgagtttgt cttccaagga aaattctctg tgaaaaaatc agtccgcttt ggcgcccgat
      181 accttgacca ctttggccca cttcacgatt tcttcacgca ggaacctgtc gaactggtcg
      241 atcggcatgc ccatggcgac cgtgccctgc ttgagccagg tttccttcgt ctcgggcgag
      301 ttgatgatct tgttcacctc ggcattgacg cgctcgagca ccgggcgcgg cgtcttcgcc
      361 ggcgccatca ggccgagcca gatgcccgac tcgtagccgg gcacgccggc ctcggcaacg
      421 gtcggaacgt tgggcgtcac gggcgagcgc ttcgcgccgg tggtggcgag cgccttcacc
      481 ttgccggcgc ttgcgctcgc ggccatggtg ggcaccgcgt cgaacatcat ctgcacctgg
      541 ccgccgagca cggcggcgcg cgcctggtcg ctgcccttgt gcgggatgtg cacgatatcg
      601 acgccggcca tcgacttgaa cagctcgccg gccatgtggt agggcgtgcc gttgcccgat
      661 gaggcgtagt tcagcgcgcc gggcttcgac ttggccagcg cgatgaattc cttgagcgtg
      721 ctcgccggca cgtcgctcgc caccaccatc atcaggtcgg agtagctcac gccggtgatc
      781 ggcgcgaggt cgcgcatcag gtcgaagggc tttttcggga tcagcgtctc gttgacggtg
      841 tgggtgttcg acatcatgag cagcgtgtag ccgtccgcat cggatttggc taccgcgtcg
      901 gtgccgacga tcgagcccgc gccagggcgg ttctcgatca ccaccggctg gcccagattg
      961 tccgacagct tggcgccgag atagcgcgcg tagaggtccg ccgagccgcc cacgccgtag
     1021 ggcacgacga tcttcaccgc cctggacgga tagctctgcg cccaggatgg agcgctgagc
     1081 gccgccagca gaaaagcgga aacgatgcgg aacatctccc ctccttaatt agaatagccg
     1141 cgggcatgag gatactcgtc accggcgcgg caggctatat cggttccgct ctcgcgcgct
     1201 tcctcgcggc gcgcaatctg caggtcgtcg ccaccgacca gtcggcgccg gctccgctga
     1261 tcggcaacat cgcctacccg cagttcgcgc gctcgctgat aacgcccgag gtgggcgcgg
     1321 tattccacct cgcctcgctc gtttcaggcg gcgccgagca gaacttcgag ctcggcacca
     1381 aggtgaacct cgacgcgacg cgcgacctgc tcgaggcctg ccgcctcgcc ggccactgcc
     1441 cgaagttcgt cttcgcttcg tcgatcgcgg tctactcggg cgccgaggtc atcaccgacg
     1501 agacgccgcc gcggccgcgc ctttcctacg gcgcgcagaa gctgatcggc gagatcctga
     1561 tcgacgacta cacgcgccgc ggctatgtcg acgggcgcac gctgcgcctg ccgaccgtga
     1621 tggtaaggcc ccaaagcgcg aataccgcgg tgtcgggctg ggcgagcgcg atcatccgcg
     1681 agccgctcgc cggccgcgac tacgtctgcc cggtgcgtcc cgagacgcgc atggcgtgca
     1741 tctcgctcgg gcgcgtggtc gaatcttttt ttcacgcttt cgaactcccc ggggagaaac
     1801 tggattctca aagaaccatc ctcctgaacg gtattcccgt atcggcgcag cagatgcgcg
     1861 acgccgcgct cggcaggggc aaggtgcgct tcgagcccga tccggcgata cagaagatca
     1921 tggacggcgt gccgcaggcc agcgcctcga agcgcgccgt cgccctgggc ttccgccaca
     1981 gcgcgaacat cgaagaaatc gtcgatgaat acaaaaaaga ccatcccgat cctgccctcg
     2041 cgcgtaacgg ttgagggcct cgaccgggcg ccgcaccggg catttctgca tgccctgggc
     2101 ctgtcggacg ccgatctgaa aaaaccgttc gtcggcgtcg cctcgaccga cggccgcgtc
     2161 acgccctgca acgcgctgct tggcgagttc gtgcgcgagg cgtgcgccgc cgtgcgcctc
     2221 gcgggaggta tgccattcga cttcgcctcc atctcggtgg cggactcgat gtcgatgaac
     2281 cacggcggca tgcgctactc gctgctgtcg cgcgagctga tcgccgacgg cgtcgaggcg
     2341 gtggcgcgcg gccacgccta cgatgcgctg gtcaccttcg ccggctgcga caagaccctg
     2401 cccggaatga tgatggccat ggcgcggctc gacctgcctt cgatcttcgt ctacggcggc
     2461 gcggcgctcc cgggaaagtg gaaaggccgc gacgtcaccg tcctcgatac ctacgaaggc
     2521 gtcggcgcgg tccttgcggg aaagatgggc gagtccgagc tgaacgagct ggagcacatc
     2581 tgcattccca ccctcggctc ctgtcccggc cagttcaccg ccaacaccat ggcgatggtg
     2641 tcggagacgc tcggcctcgc gcttcccggc tcggccacca tgccggcggt tgcaaccgag
     2701 cgcagcgagg ttgcccgccg tgcgggaaaa atcgtaatgg aactgctaag gaacggcgga
     2761 ccgctgccga aggatctcat cacaaagaag agccttgaga acgcctgcgc cgcggtcgcc
     2821 gccaccggcg gctcgaccaa cgccgcgctg cacataccgg cgattgccca cgaggccggc
     2881 atccgcttca cgatggatga cttcgcgcgc gtcgccaagc gcacgccgct tctcgccgac
     2941 atgaagcccg gcgggcgatt cctcgcgaag gacctgcacg ccgtcggcgg tgtttacgct
     3001 gtcttgaagg ctctgctggc gcacggcgcc ctccacggcg attgcatgac cattttcggt
     3061 tccaccctcg aagacgagct gcgcgagcgt ccggaggcgg acggtgaggt cgtcagaaaa
     3121 actccgatct cgccgacagg cggactcgtg gtgctgaaag gaaatctcgc cccgcaaggc
     3181 gcgctcatca aagtcgcggg cctcgagtcg ctggtgttcg agggtcgggc gcgcgtgttc
     3241 gattccgaag aggcctgcgc ggaagtcgtc cgaaaacgca catataagga aggcgaggtg
     3301 ctggtcatcc gctacgaagg cccgaagggc gggccgggca tgcgcgagat gctcggcgtc
     3361 actgcgcttc tatatggtca gggcgtgggg gagaaggtgg cgctgctcac cgacgggcgc
     3421 ttctcgggcg cgacgcgcgg catgatggtc ggctacgcct cgcccgaggc ggccgccggc
     3481 ggggccctgt cgatcgtgaa
//
LOCUS       DASYJA010000004         4373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1635203, whole genome shotgun sequence.
ACCESSION   DASYJA010000004 DASYJA010000000
VERSION     DASYJA010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635203"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..544
                     /locus_tag="VGO02_00080"
     CDS             92..544
                     /locus_tag="VGO02_00080"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEV7476112.1"
                     /translation="MVDHEGPGGKPATLSQSSAILLYCAEKSGKHLPKKPADQPAFLE
                     ALMRASTDMTPTLGAIFNSSKVKDAHAPTVDWFKDLWKRYLKVWDDLLAKRRYAAGNE
                     FTIADISLYAGYARMQKAIPDLVEGCPNVERWAKEVGARPATQRALKF"
     gene            complement(541..840)
                     /locus_tag="VGO02_00085"
     CDS             complement(541..840)
                     /locus_tag="VGO02_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476113.1"
                     /translation="MRVLILCILAAASLPALAENQLTDRERSILAQQQQQREVMLARA
                     KQRCIEQRGVDCDTEQGLQEWVLLERSRESAVLDRITPLPVPSSGSGATTPAPGR"
     gene            856..1167
                     /locus_tag="VGO02_00090"
     CDS             856..1167
                     /locus_tag="VGO02_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476114.1"
                     /translation="MNDPGFFLVVRFWVAPQAESAVLGWLDGGHVAEVLRQPGFLWCK
                     RIRLEKAADGWAGFSQIYAIESKAAFDAYNTSPVMEKFKRERAPFEQHMRIDRFFGQV
                     D"
     gene            complement(1155..1853)
                     /locus_tag="VGO02_00095"
     CDS             complement(1155..1853)
                     /locus_tag="VGO02_00095"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7476115.1"
                     /translation="MTYVLIHGAWHGGWCWRPVAALLRKAGHEVHAPSLTGLGERKHL
                     GGGRVDLDTHIQDVVGLIEMEDLHNVVLVGHSYGGMVITGAADRVPARIARLVYLDAF
                     VPEDGKCLLDYAVPERAARMRHEGERTGFVTPPLMSLWGLTEQKHLDFVGPREVRHPY
                     ATMAQPIRLGNFAEWRAIPKAFVYCSSPATGSFDQFAAKYREDPAWRFHELKTGHDAM
                     ILMPGEVARLLQST"
     gene            complement(1865..2719)
                     /locus_tag="VGO02_00100"
     CDS             complement(1865..2719)
                     /locus_tag="VGO02_00100"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEV7476116.1"
                     /translation="MDDLPKARLRRSRLFRVAWIVPLVAAAVAGWLVYERMRAFGPEV
                     TIQFNQGAGLRIGQTPVKYRGVPIGEVTGVELSKDHKHVLVTARLRRSAALVASEGAI
                     FWIVRPQVGFGSVTGLNTVLSGPEIEVRPGEGEPRRNFVGRETAPLEENGLRLVLRAE
                     RPKSLRANSPVYYRGIEVGTVQKLELAANAASADVTVLIQSRYAPLVREGSAFWDTSG
                     ISVKGGILKGLEVEFESLRALATGGVEFASPPNSPRAKPGTAFFLHDEPKKEWLAWQA
                     KIPLAAEK"
     gene            complement(2692..3312)
                     /locus_tag="VGO02_00105"
     CDS             complement(2692..3312)
                     /locus_tag="VGO02_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011350988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEV7476117.1"
                     /translation="MASVENIVACPVCALAQRVEAGRPGVVFECARCGAYIGREPERE
                     SLHLTAALTLAALVLYVPANLFPILRMYWSGAYSESTVWDGVVSLAHANQWFVAVVVF
                     LASIVIPLVKLASLLYLVASAQLRSRALPHLRTRLWRFVDVIGPWAMLDVFLLAVLVA
                     LVKLRTLATVLPGPGLAAFCAVVVLTILASATFDPRLIWTTSQKPA"
     gene            3361..>4373
                     /locus_tag="VGO02_00110"
     CDS             3361..>4373
                     /locus_tag="VGO02_00110"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7476118.1"
                     /translation="MTPPQSFAALRHPGYRAYFFGAAVAMMGDSIEHVISYWILFQKF
                     ESQALAGFAVLSHWLPFLLFSLYSGSLADRFDPRRLIQLGMALFMLASLGWALLFLFD
                     VLQMWHAVALLTLHGFAGVLWAPAAQLFIHQLVGERELHSAIRLTAIARWLGLLAGPA
                     VGGVILLLMGPSYGLLFNVLIYLPLVLWLWKAPFNPVRLRVPALRSFSEVAAIARAIA
                     ADRTIVSMTLLAGGASLLVGNAYQAQMPEFAHYLGHGDADITYSILLGADAAGALTAG
                     LVLEGRGLLQANPRTAFLLAMLWCVAIAAFAVSTSYALALALLFAAGFLELSFNAMAQ
                     TLV"
BASE COUNT          670 a         1523 c         1475 g          705 t
ORIGIN      
        1 gccctacaac ttccacccca tcaacctgga caagggcgag aacaagacgc cgcagttcct
       61 cgccatgaac ccgatggggt agatcccggt catggtcgac cacgaggggc ccggcggcaa
      121 gccggcgacc ctctcccaat cgagcgccat cctgctgtac tgcgccgaga aatcgggcaa
      181 gcatctgccg aagaagccgg ccgaccagcc ggccttcctc gaggcgctga tgcgcgcgtc
      241 gaccgacatg acgccgaccc tcggggcgat cttcaattcc tcgaaggtga aggacgcgca
      301 cgcgcccacc gtcgactggt tcaaggactt gtggaagcgc tacctgaagg tctgggatga
      361 cctgctcgcc aaacgcaggt acgccgccgg caacgagttc acgatcgccg acatctcgct
      421 gtatgcgggc tacgcgcgca tgcagaaggc gatccccgat ctggtcgagg gctgcccgaa
      481 tgtcgagcgc tgggcgaagg aggtcggcgc ccggccggct acccagcgcg cgctgaagtt
      541 ctagcggccc ggcgccggcg tggtcgcgcc tgagccggac gagggcaccg gcagcggcgt
      601 gatgcggtcg agcaccgccg actcgcggct gcgctcgagc agcacccact cctgcaggcc
      661 ctgctcggta tcgcaatcga cgccgcgctg ctcgatgcac cgctgcttgg cgcgagccag
      721 catgacctcg cgctgttgct gctgctgggc gaggatcgag cgctcgcgat cggtgagctg
      781 gttttcggcc agcgcaggca gcgacgccgc ggccaggatg caaaggatga ggactctcat
      841 gccccttcga cagcaatgaa cgatcccggt ttcttcctgg tggtgcgttt ctgggtcgcg
      901 ccgcaggccg agagcgcggt gctgggctgg ctggacggcg gccatgtagc cgaagtgcta
      961 cgccagccgg gcttcctgtg gtgcaaacgg atcaggctgg agaaagcggc cgacggctgg
     1021 gccggctttt cgcagatcta tgccatcgag tccaaggcgg ccttcgacgc ctacaacacc
     1081 tcgccggtca tggagaaatt caagcgggag cgggcgccct tcgaacagca catgcgcatc
     1141 gaccgcttct tcggtcaggt cgattgaagc aggcgcgcga cttccccggg catcaggatc
     1201 atggcgtcgt gcccggtctt cagctcgtgg aagcgccagg ccgggtcttc gcgatacttc
     1261 gccgcgaact ggtcgaagga gccggtggcg ggcgacgagc aatagacgaa ggccttggga
     1321 atggccctcc actccgcgaa attacccagc ctgattggct gcgccatcgt ggcgtaggga
     1381 tgcctgacct cgcgcggccc gacgaagtcg aggtgcttct gctcggtcag gccccagagc
     1441 gacatcaggg gcggcgtgac gaagccggtg cgctcgccct cgtggcgcat gcgcgccgcg
     1501 cgctcgggca cggcgtagtc gagcaggcat ttgccgtcct ccggcacgaa ggcgtccagg
     1561 tagaccagcc gcgcgatgcg cgccggcacg cggtctgcgg cgccggtgat caccatgccg
     1621 ccatagctgt ggccgaccag aaccacattg tgcaggtcct ccatctcgat cagcccgacc
     1681 acatcctgga tgtgcgtgtc gaggtcgacc cggccgccgc cgagatgctt gcgctcgccc
     1741 aggccggtga gcgagggcgc gtgcacctcg tggccggcct tgcgcaagag cgcagctaca
     1801 ggacgccagc accagccgcc gtgccaggcg ccatgaatca atacgtaggt cacgccggct
     1861 ttttttactt ttcggcggcg agcgggattt tcgcctgcca ggcgagccat tccttcttcg
     1921 gctcgtcgtg caggaagaag gcggtgccgg gcttggcgcg cggcgagttg ggaggggaag
     1981 cgaactccac tccgcccgtg gcgagcgccc gcagcgattc gaactccact tcgaggcctt
     2041 tcaaaatccc ccccttcacg ctgatgccgc tggtgtccca gaacgcggag ccctcgcgca
     2101 ccagcggcgc gtagcggctc tggatcagca cggtgacgtc ggccgaggcg gcgttggcgg
     2161 cgagctcgag cttctgcacc gtgccgacct cgatgccgcg atagtacacc ggcgaattcg
     2221 cccgcagcga cttgggccgt tcggcgcgca gcaccaggcg caggccgttc tcttccagcg
     2281 gcgcggtctc gcggccgacg aagttgcggc gcggctcgcc ttcgccgggg cgcacctcga
     2341 tctccggccc gctcagcacg gtgttcaggc cggtgacgct gccgaagccg acctgcggcc
     2401 gcacgatcca gaagatcgcg ccttcgctgg ccaccaaggc cgccgagcgt cgcagccgcg
     2461 ccgtcaccag cacgtgcttg tggtccttgg agagctcgac gccggtgacc tcgccgatcg
     2521 gcacgccgcg gtacttcacc ggcgtctggc cgatgcgcag cccggcgccc tggttgaact
     2581 ggatggtgac ctccggcccg aaggcgcgca tgcgctcgta gaccagccag cccgccaccg
     2641 cggcggcgac cagcggcacg atccaggcga cgcggaacag gcggctgcgc ctcaggcggg
     2701 cttttgggag gtcgtccaaa tgaggcgcgg atcgaaggtg gcggaagcga ggatggtcag
     2761 cacgaccacc gcgcagaagg cggcgaggcc cggcccggga agcaccgtgg cgagggtgcg
     2821 cagcttgacc agcgccacca gcaccgcgag caggaacacg tcgagcatcg cccaggggcc
     2881 gatgacgtcg acgaagcgcc acaggcgcgt gcgcaggtgc ggcagcgcgc gcgagcgaag
     2941 ctgcgcgctg gcgacgaggt acagcagcga ggcgagcttg accagcggga tgacgatgct
     3001 ggcgaggaac accaccacgg cgacgaacca ctggttggcg tgcgccaggc tgaccacgcc
     3061 gtcccagacc gtgctctcgg agtaggcgcc gctccagtac atcctcagga tcggaaagag
     3121 gttggccggt acgtacagga ccagggcggc aagggtcagg gcggcggtga ggtgcaggct
     3181 ctcgcgctcg ggctcgcggc cgatgtaggc gccgcagcgc gcgcactcga acaccacccc
     3241 gggacggccg gcttcgaccc gctgcgccag ggcgcagacc gggcaggcga cgatgttctc
     3301 cacggaggcc atttcgagta gaccgcgttc cttctttggt gtttccccta aagtacgcgc
     3361 atgacacccc cgcagtcctt cgccgccctg cgccatccgg gctaccgcgc ctacttcttc
     3421 ggcgccgcgg tggccatgat gggcgacagc atcgagcacg tgatcagcta ctggatcctg
     3481 ttccagaagt tcgagtcgca ggcgctcgcc ggcttcgccg tgctgtcgca ctggctgccg
     3541 ttcctcctgt tctccctcta ctcggggtcg ctcgccgacc gcttcgaccc gcgccgcctg
     3601 atccagctcg gcatggccct gttcatgctc gcctcgctcg gctgggcgct gctgttcctg
     3661 ttcgatgtgc tgcagatgtg gcacgcggtg gcgctgctga cgttgcacgg cttcgccggg
     3721 gtgctgtggg cgccggccgc ccagctcttc atccaccagc tggtcggcga gcgcgagctg
     3781 cacagcgcca tccggctcac ggcgatcgcg cgctggctgg ggctgctcgc cggccccgcg
     3841 gtcggcggcg tcatcctcct cctgatgggc ccctcctacg ggctgctgtt caacgtcctc
     3901 atctacctgc cgctggtcct gtggctgtgg aaggcgccgt tcaaccccgt ccggctgcgc
     3961 gtgccggcgc tgcgcagctt ctcggaggtc gccgcgattg cccgcgccat tgcagccgac
     4021 cgcaccatcg tctcgatgac gctgctcgcc ggcggcgcct cgcttctcgt cggcaacgcc
     4081 taccaggcgc agatgccgga gttcgcgcac tacctcggcc acggcgacgc cgacattacc
     4141 tatagcatcc tgctcggcgc cgatgccgcc ggagcgctca ccgctggact ggtgctcgag
     4201 ggccgcggcc tgctgcaggc caacccgcgc accgcgttcc tgctcgccat gctctggtgc
     4261 gtcgccatcg cagccttcgc cgtgtcgacc tcgtatgcgc tcgcgctggc gctgctcttc
     4321 gccgccggct ttctcgagct ctcgttcaac gccatggcgc agacgctggt gca
//
LOCUS       DASYJA010000005         6194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_818540, whole genome shotgun sequence.
ACCESSION   DASYJA010000005 DASYJA010000000
VERSION     DASYJA010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818540"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1738)
                     /locus_tag="VGO02_00115"
     CDS             complement(<1..1738)
                     /locus_tag="VGO02_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476119.1"
                     /translation="MERRALLTIFLVPAALCAVWTVLAGKDVNWDLLNYHYYLGYELV
                     GGRLGLDFFAASGQSYLNPVGYLPFYLMVAAGWHSIAASATLAVAHSTSLALLYLLAW
                     QLFAHLPHRERVVLCLLATALGGATSVWWATVGASFLDPLLVPPVLAGLALLLEGGPR
                     PARRAALAGALFGAAAALKYSNALYALAALPLALSLPGLTRAHRLRAGLAYAAGGAAA
                     VALLAGPWHAALWREFGNPVFPMLNAWFQSPHALPINLAGGRFAPGDLAAAAAFPFRM
                     ALLDRSLYTETFAPDFRFAALAVVGVALAAAALRRGAPDPRALRGADWRLLGFFAAAA
                     ALWLASSANARYGLAVLLLAGIVLARLTERLLPRGAARVALAALLAVQIAASMVAAHP
                     RWYLAEPWTRHWLPYAPPERALREPALYLSIEIQSMSVVAPFLHPASAFVNFRGQHSL
                     PSDSPRLAALLERYRGRVRALGRGLELVDGRPRAQEVAVYDATLRRIGYRVDAEDCFA
                     IAWRRDDEDALSRAANRLALGRTDAEPLSVASCALRPQARDPADAEAERRMSALFDRI
                     EKACPGLFRGQTA"
     gene            complement(1749..3065)
                     /locus_tag="VGO02_00120"
     CDS             complement(1749..3065)
                     /locus_tag="VGO02_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476120.1"
                     /translation="MLSRASYSEVDERLSASWLDRLRFFPFLGSQFLYQFAYAGAALA
                     ALGCVVQWRAWGWRVSAFLLTAFLMPSAGLVLLLKFDYDSFQKYMFHVYPLPSYAVGA
                     LWLALGFAWLAQRYALRRAAASAVAAVLVGVMLAWGARTNLLAGYDWSARYAQTILRT
                     LPPNATVFVQGDADLGPIAYFHIIENWRPDITLYQPKGLVLGNRRFHPLRIDQESANR
                     AIREVVDSAPGPVVFTLDSYTRYARRDRWLFTVLDKSSPDTTVVTIDIPEEAVRFFEE
                     SVAVVDEPNGWAAFFQGELRRRYALLLAQSLQQRGKAADARTLRQLDVLSKDFYGALG
                     LAEGLLGSKASYSSGAVAGLLDTVRATMPADVPKLHLSRFFYLRGALRLDLRERPGAI
                     GDFETALSTWPVPENPALAPLRDLYREAGDNAALAAVEERVHRRKR"
     gene            complement(3129..3782)
                     /locus_tag="VGO02_00125"
     CDS             complement(3129..3782)
                     /locus_tag="VGO02_00125"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEV7476121.1"
                     /translation="MLAPELALRPAKGDWWRTAAVAGALFILYALTSPRTVSAEDDSL
                     FILSAYFLGIEHAPGYPLFILAGKLFTLLPIGSIAYRVHLASAFFGALACGAAWLCAR
                     ALTGQRLAAYLAAFGLGLSPVFWSQAIIAEVYTLNALFFLALVFLGLQALEGRRVLPW
                     MAFVFGLSLSNHWPLMLLVAPGFAILLWPRLPEMLRRLPLLALVFAAGLLPYAWMVY"
     gene            complement(3782..5755)
                     /locus_tag="VGO02_00130"
     CDS             complement(3782..5755)
                     /locus_tag="VGO02_00130"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEV7476122.1"
                     /translation="MTPAQFTPQRRDWATAGALAAALFVLYAASAPRGVALEDDGLFV
                     LSSYFLGIEHPPGYPLHTLLGRLFTLLPVGSVAYRVHLLSALFGAAACGMLWMCARTL
                     VEGRAPAYVAALGLGLMPVFWSQSVIAEVYTLNAFFFVLLIWLGLRASAGLLYWIALV
                     FGLSLSNHWPLMGLVFPAFLILLWPLRAEVVKRLGALSWLVILGLLPYAWMIYRSWRA
                     LPISFYGPLETLPEIWFFLSRAGYAGIDESPTGGWLDRIRFLQFLGGEMFVQLAVAGT
                     LLAAAGFAAQWRSLGRRVSAFLTVAFLMPSVVLIFLLRFDYDYLSRHVFHVYPLPSYA
                     VAALWMALGFDWLSRRLALRAAQRIAVACVVLALIGALGARTNLDPNQAWAERYARMV
                     LKTLPRDAVVFPQGDADLPPIAYLHFVENERPDVTLYQANGLILGNRLFHLLRVSDAE
                     AQARLKAFIATERAPLVSTMVPLEGYALRDRWLHVEVDRAARDPSRLTVEIPEEATVF
                     FEQEVLHDSAPHNAWVAYHRNELRFRYALLLARKLVPGQAADERSRRHLAVLENDRYG
                     ALGLAEGMMGNPAGYSPAAVAGLLERVRELMPSDELKMHRAKYFYVRAALRLGLGERA
                     GAMGDLETAISVWPARANPAFKALDNVRAAGGG"
     gene            complement(5752..>6194)
                     /locus_tag="VGO02_00135"
     CDS             complement(5752..>6194)
                     /locus_tag="VGO02_00135"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV7476123.1"
                     /translation="FFMLILYHLTGRLLPARFFLFAAVGVTGIGVHLVVLSTTFHALG
                     DNFLLSQALATWAAMTSNFFLNNAFTYADQALRGARIWRGLASFYAACSIGAFINIAL
                     AEWLYLQAMPFWAAGIAGALIAALWNFFTTASFTWGGPSAGKRG"
BASE COUNT         1124 a         2093 c         2281 g          696 t
ORIGIN      
        1 ccgcggtctg gccgcggaac aggcccgggc aggccttctc gatgcggtcg aagagcgccg
       61 acatgcgccg ctccgcctcg gcgtcggcgg gatcgcgcgc ttgcgggcgc agcgcgcagc
      121 tcgcgaccga gagcggctcg gcgtcggtac gcccgagcgc gaggcggttc gccgcgcgcg
      181 acagcgcgtc ctcgtcgtcg cggcgccacg caatggcgaa gcagtcctcg gcatcgacgc
      241 ggtagccgat cctgcgcagc gtggcgtcgt agaccgccac ctcctgcgcg cgcggccggc
      301 cgtcgacgag ctccaggccg cggccgagcg cgcgcacgcg gccgcggtag cgctcgagca
      361 gcgcggcgag ccgcggcgag tcgcttggca ggctgtgctg gccgcggaaa ttgacgaacg
      421 cggaagcggg gtgcaggaac ggcgccacca ccgacatcga ctggatctcg atgctgaggt
      481 agagggccgg ctcgcgcagc gcgcgctcgg gcggcgcgta cggcagccag tgccgcgtcc
      541 agggctcggc caggtaccag cgcggatgcg cggcgaccat cgaagcggcg atttgaacgg
      601 cgagtaaagc ggcgagcgca acgcgcgcgg cgcccctcgg cagcaggcgc tccgtgagcc
      661 gtgcgaggac gatgccggcg agcagcagga ccgcgaggcc gtagcgcgcg ttcgccgagc
      721 tcgccagcca gagagcggcg gcggcggcga aaaagccgag caagcgccag tcggcgccgc
      781 gcagggcgcg cggatcgggc gcgccgcggc gcagcgcggc cgcggcgagc gcgacgccaa
      841 ccacggcgag agccgcgaag cggaaatccg gcgcgaaggt ttccgtgtac agcgagcggt
      901 cgagcagcgc catgcgaaac gggaaggcgg cggcggcggc gaggtcgccc ggcgcaaagc
      961 ggccgccggc gaggttgatc ggcagcgcgt gcggcgactg gaaccacgcg ttgagcatcg
     1021 ggaacaccgg gttgccgaat tcgcgccaca gagccgcgtg ccagggtccg gcgagcagcg
     1081 ccacggcggc ggcgccgccc gcggcgtagg ccaggcccgc gcgcagccga tgcgcccgcg
     1141 tcagcccggg cagcgacagg gcgagcggca gcgcggcgag cgcgtagagg gcgttcgagt
     1201 acttcagggc cgccgccgcg ccgaagagcg caccggcgag cgccgcgcgc cgcgccggcc
     1261 gcgggccgcc ttccagaagc agcgcgaggc ccgccagcac cggcggcacc agcagcggat
     1321 cgaggaacga agcgccgacc gtggcccacc agacggaggt ggcgccgccg agcgcggtag
     1381 cgagcaggca gaggacaacg cgctcgcgat gcggcagatg agcaaacagc tgccacgcca
     1441 gcaggtacag cagcgcgagg ctggtgctgt gcgccacggc gagcgtggcc gaggccgcta
     1501 tgctgtgcca gcccgccgcc accatcaggt aaaagggcag gtagccgacc gggttgaggt
     1561 agctctgccc gcttgcggcg aagaaatcca ggccgagccg gcccccgacc agctcgtagc
     1621 ccaggtagta gtggtaattc agcagatccc agttcacgtc cttgccggca aggacggtcc
     1681 acaccgcgca cagcgcggcg ggcaccagga aaatagtcag gagagcgcgg cgctccatgg
     1741 ctgcggcgct accgcttgcg gcggtgcacg cgttcctcca cagccgcgag cgccgcgttg
     1801 tcgccggctt cgcggtacag gtcgcgcaat ggggccagcg ccgggttctc cggcaccggc
     1861 caggtcgaca gcgcggtctc gaagtcgccg atcgcgccgg ggcgctcgcg caggtccagg
     1921 cgcagcgcgc cgcgcaggta gaagaagcgc gagaggtgca gcttcggcac gtcggcgggc
     1981 atggtggcgc gcaccgtgtc gagcaggccg gcgaccgcgc ccgacgaata gctcgccttg
     2041 ctccccagca gaccttcggc cagcccgagc gcgccgtaga agtccttcga cagcacgtcg
     2101 agctggcgca gggtgcgcgc gtccgccgcc ttgccgcgct gctgcagcga ctgcgcgagc
     2161 agcagcgcgt agcggcggcg cagctccccc tggaagaaag ccgcccagcc gttgggttcg
     2221 tcgaccacgg caaccgattc ctcgaagaaa cgcaccgcct cctccgggat gtcgatggtc
     2281 actacggtgg tgtccggcga ggacttgtcg agcaccgtga acagccagcg gtcgcgccgc
     2341 gcgtagcgcg tataggaatc gagcgtgaag accaccgggc ccggcgccga gtcgaccacc
     2401 tcacggatcg cgcggttcgc gctttcctgg tcgatgcgca gcggatggaa gcgccggttg
     2461 ccgagcacca ggcccttcgg ctggtagagc gtgatgtcgg gccgccagtt ctcgatgatg
     2521 tgaaagtagg cgatcggccc gaggtccgcg tcgccctgca cgaacaccgt cgcattcggc
     2581 ggcagcgtgc gcaggatggt ctgcgcatag cgcgcggacc agtcgtagcc ggcgagcagg
     2641 ttggtgcgcg cgccccacgc cagcatgacc ccgacgagca ccgcggcgac cgcgctcgcc
     2701 gcggcgcggc gcagcgcata gcgctgcgcg agccaggcga agcccagcgc cagccagagc
     2761 gcgcccacgg catacgaagg cagcgggtag acatggaaca tgtacttctg gaagctgtcg
     2821 tagtcgaact tcagcagcag caccaggccg gcgctgggca tgaggaaggc ggtcagcagg
     2881 aaggcggaaa cgcgccaacc ccatgcgcgc cattgcacca cgcagcccag cgccgcgagc
     2941 gcggcgccgg catacgcgaa ctggtagagg aattgagagc cgaggaacgg gaaaaagcgc
     3001 aggcgatcga gccaggaagc tgacagccgc tcgtccacct cggagtagga ggcacggctc
     3061 agcatgtgcc acacctcgcc ccagctctcc agcggcccga ggtagctgat gagcggctcc
     3121 tgccacgttc aatagaccat ccaggcgtag ggcagcaggc ccgcggcgaa gacgagcgcg
     3181 agcagcggca ggcgccgcaa catttcaggc aaccgcggcc acaggagaat cgcgaagccc
     3241 ggcgcgacca ggagcatcag cggccagtga ttgctcaggc tcaggccgaa gacaaacgcc
     3301 atccacggca gcacgcgccg gccctcgagc gcctgcaggc cgagaaatac cagagccagg
     3361 aaaaacaatg cgttgagcgt gtagacctcg gcgatgatgg cctgcgacca gaacaccggc
     3421 gacaggccca ggccgaacgc ggcgaggtag gctgcgaggc gctggccggt gagcgcgcgg
     3481 gcgcatagcc acgcggcgcc gcaggcgagc gcgccgaaga acgcgctcgc cagatgcacg
     3541 cggtaggcga tcgagccgat cggcagcagc gtgaacagct tgcccgccag gatgaacagc
     3601 ggataccccg gggcgtgctc gatccccagg aagtaggcgg ataggatgaa caggctatcg
     3661 tcctctgcag acacggtgcg gggcgaggtg agcgcataga ggatgaaaag agcgccggcc
     3721 acggccgcgg tcctccacca gtcgcccttc gcgggccgca acgcgagctc gggcgcgagc
     3781 atcagccgcc ccccgctgcg cgcacgttgt ccagcgcctt gaacgcagga ttggcgcgcg
     3841 ccggccagac cgagatcgcg gtctcgagat cgcccattgc gcccgcgcgc tcgccgaggc
     3901 ccaggcgcag cgccgcgcgc acgtagaaat acttggcgcg gtgcatcttg agctcgtcgg
     3961 agggcatcag ctcgcgcacc cgctcgagca ggccggcgac cgccgccggc gaatagcccg
     4021 cggggtttcc catcatgcct tcggcgaggc cgagcgcgcc gtagcggtcg ttctccagga
     4081 cggcgaggtg gcggcggctg cgctcgtcgg ccgcctgccc cggcacgagc ttgcgcgcga
     4141 gcagcagcgc atagcggaag cgcagctcgt tgcggtggta cgccacccat gcgttgtgcg
     4201 gtgcgctgtc gtgcagcact tcctgctcga agaagacggt cgcctcctcg gggatctcga
     4261 ccgtgagccg cgacggatcg cgcgccgcac ggtcgacctc gacgtgcagc cagcggtcgc
     4321 gcaacgcata gccttcgagg ggcaccatcg tgctcaccag cggcgcgcgc tcggtcgcga
     4381 tgaacgcctt cagcctggcc tgcgcctcgg cgtcgctcac ccgcagcagg tgaaacaggc
     4441 gattgccgag gatcaggccg ttcgcctggt agagcgtgac gtcggggcgc tcgttctcca
     4501 cgaaatgcag gtaggcgatg ggcggcaggt cggcgtcgcc ctggggaaat accaccgcgt
     4561 cacgcggcag ggttttcagc accatccggg cgtagcgctc ggcccacgcc tggttcgggt
     4621 cgaggttggt acgcgcgccg agcgcgccga tcagcgcaag caccacgcag gccacggcga
     4681 tccgctgcgc cgcgcgtagc gcgagccggc gtgaaagcca gtcgaaaccg agcgccatcc
     4741 agagcgccgc caccgcgtag gaaggcagcg gatagacgtg gaacacgtgt ctagacaaat
     4801 agtcgtagtc gaaacgcagc aggaagatga gaaccaccga gggcatgagg aacgccacgg
     4861 tgaggaacgc cgacacgcgg cggccgagcg agcgccattg ggcggcaaat cccgccgccg
     4921 cgagcagcgt tcccgccacc gccagctgga cgaacatctc gccgccgagg aattgcagga
     4981 agcggatgcg gtcgagccag ccgccggtgg gcgactcgtc gattcccgcg tagccggcgc
     5041 ggctgaggaa gaaccagatc tccggcagcg tctcgagcgg gccgtagaag ctgatcggga
     5101 gcgcgcgcca cgagcggtag atcatccacg cgtagggcag gaggccgagg atcaccagcc
     5161 acgacagcgc gcccaggcgt ttgacgactt ccgcacgcag cggccacagc aggatcagga
     5221 acgccggaaa gacgaggccc atcagcggcc agtggttgct gaggctcagg ccgaagacca
     5281 gcgctatcca gtacagcaac ccggcgctcg cgcgcaggcc aagccagatc agcagcacga
     5341 agaagaaggc gttcagggtg tacacctcgg cgatcaccga ttgcgaccag aacaccggca
     5401 tgaggcccag gccgagcgcg gcgacgtagg ccggcgcgcg cccctcgacc agcgtgcggg
     5461 cgcacatcca gagcatgccg caggcagccg cgccgaacag cgcgctaagc aggtgcaccc
     5521 ggtacgccac cgagccgacc ggcagcagcg tgaacagcct gccgagcagc gtatgcagcg
     5581 ggtagccggg cgggtgctcg atgccgagga agtagctcga caggacgaac aggccgtcgt
     5641 cctccagggc gacgccgcgc ggcgcgctcg ccgcgtagag gacgaagaga gccgcggcga
     5701 gcgcgccggc cgtggcccag tcccgccgct gcggcgtgaa ctgcgccggc gtcatccgcg
     5761 cttgccggcg ctcgggccgc cccaggtgaa cgaggcggtg gtgaagaagt tccacagcgc
     5821 cgcgatcagc gccccggcga tgccggcggc ccaaaaaggc atggcctgca ggtacagcca
     5881 ttcggcgagc gcgatgttga tgaaggcgcc gatgctgcag gcggcgtaga agctggcgag
     5941 gccgcgccag atgcgcgcgc cgcgcagcgc ctggtcggca taggtgaagg cgttgttgag
     6001 gaagaagttg ctggtcatcg ccgcccaggt ggcgagcgcc tggctgagga ggaaattgtc
     6061 gccgagcgcg tggaacgtcg tcgacagcac cacgaggtgc acgccgatgc cggtgacgcc
     6121 gaccgcggcg aagaggaaga agcgcgccgg caggagccgt ccggtgaggt gatagaggat
     6181 cagcatgaag aact
//
LOCUS       DASYJA010000006         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2271031, whole genome shotgun sequence.
ACCESSION   DASYJA010000006 DASYJA010000000
VERSION     DASYJA010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2271031"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VGO02_00140"
     CDS             <1..654
                     /locus_tag="VGO02_00140"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEV7476124.1"
                     /translation="GWHRELWNWLFWAGSFLAAFAQGLMLGRYITGFQGGAGYWLFAL
                     VTGAALCGGYVLLGATWLILRCEGALRDKAVAWARWGLLWVALGVALVSLGTPLVSET
                     VREKWFSFPRIVFLSILPIATVAAWLWVWFSLKKSDTKPFFGAIAIYVLAFLGLAYSL
                     FPYVIIDRMTIWQAAAHESALKFVLAGAVVVLPFIVGYTFYVYRVFGGKTRGGDPYG"
     gene            647..1843
                     /locus_tag="VGO02_00145"
     CDS             647..1843
                     /locus_tag="VGO02_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEV7476125.1"
                     /translation="MDEARRHFVGGVAGAGALAALGIRHAGAQAEPAGTIPVESVKAE
                     KAKAMQWHSERPLTGSAPAHEHDFEVTPNDRMFVRNNLLIPEIDFKAHRIKITGLVNN
                     ELEFSMDELYKSFQGITLQGMLECAGSGRSAYLPRASGTPWLETGGMGCPRWSGVRLR
                     DVLAAAGVKPGGAHVAGQGGDPGMVASAAPVIRSIPLAKAMEPNTLVAWAMNGEPLPR
                     VHGYPLRLVVPGWVGSASTKWLHTLTVLDAPFKGTYMTSSYVMPKEPVEPGQKLPAET
                     LSAEAWPVKSIITSPAPNARLKAGAQATLRGRAWVGEGELERVEVSLDEGKSWQRAQL
                     WRNEGGKYAWRRFTYDFTPARAGYTTLVARAWDAAGNVQPAIPAWNPLGYFWNGWHRV
                     GVVVEA"
     gene            1833..>2038
                     /locus_tag="VGO02_00150"
     CDS             1833..>2038
                     /locus_tag="VGO02_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476126.1"
                     /translation="MRREGLVFLLLLGIQFSAEAQDRLISGPGSTLTENKCKICHELQ
                     HIRRSPLSRGEWADNIRNMKERGTP"
BASE COUNT          307 a          679 c          722 g          330 t
ORIGIN      
        1 ggctggcacc gcgagctgtg gaactggctg ttctgggccg gctcgttcct cgccgccttc
       61 gcgcagggcc tgatgctcgg gcgctacatc accggcttcc agggcggcgc cggctactgg
      121 ctgttcgcgc tcgtcaccgg cgccgcgctg tgcggcggct acgtgctgct cggcgccacc
      181 tggctgatcc tgcgctgcga aggcgcgctg cgcgacaagg cggtcgcctg ggcgcgctgg
      241 ggcctgctgt gggtcgcgct cggcgtcgcg ctggtgagcc tcggcacgcc gctggtcagc
      301 gagacagtca gggagaaatg gttttctttc cccaggattg tttttttatc aatattgccc
      361 atagcaaccg ttgctgcgtg gctgtgggtg tggttcagct tgaaaaagtc ggatacaaaa
      421 ccattcttcg gagcgatcgc gatctacgtg ctcgcgtttc tgggcctggc gtacagcctg
      481 ttcccgtacg tgatcatcga ccgcatgacg atctggcagg cggcggcgca cgagagcgcg
      541 ctcaagttcg tgctggcggg cgccgttgtg gtcctgccgt tcatcgtcgg ctacacgttc
      601 tacgtctacc gcgtgttcgg cggcaagacc aggggaggcg acccgtatgg atgaagcgcg
      661 gcgccatttc gtcggcgggg tcgccggcgc gggcgcgctc gccgcgctcg gcatccggca
      721 cgccggcgcg caggccgagc ccgccggcac catcccggtc gaatcggtca aggccgagaa
      781 ggcgaaggcg atgcagtggc actcggagcg gccgctcacc ggcagcgcgc cggcgcacga
      841 gcacgacttc gaggtcacgc cgaacgaccg catgttcgtg cgcaacaacc tgctcatccc
      901 ggagatcgac ttcaaggcgc accgcatcaa gatcaccggc ctcgtcaaca acgagctcga
      961 gttctcgatg gacgagctgt acaaatcatt ccagggcatc accctgcagg gcatgctgga
     1021 atgcgccggc tcggggcgct cggcctacct gccgcgcgcg agcggcacgc cgtggctcga
     1081 gaccggcggc atgggctgcc cgcgctggag cggcgtgcgg ctgcgggacg tcctggccgc
     1141 cgccggcgtg aagccgggcg gcgcgcacgt cgccggccag ggcggcgatc cgggcatggt
     1201 ggcgagcgcc gcgccggtga tccgctcgat cccgctcgcc aaggcgatgg agccgaacac
     1261 cctcgtcgcc tgggcgatga acggcgagcc gctgccgcgc gtgcacggct acccgctgcg
     1321 cctggtggtg ccgggctggg tcggctcggc atcgacgaaa tggctgcaca cgctgacggt
     1381 gctcgacgcg ccgttcaagg gcacctacat gacctcgagc tacgtcatgc cgaaggagcc
     1441 ggtcgagccc gggcagaagc tgccggccga gacgctgtcg gccgaggcct ggccggtgaa
     1501 gtcgatcatc acctcgccgg cgcccaacgc gcgtctgaag gccggcgcgc aggccacgct
     1561 gcgcggccgc gcctgggttg gcgaaggcga gctcgagcgc gtcgaggtgt cgctcgacga
     1621 gggcaagagc tggcagcgcg cgcagctctg gcgcaacgag ggcggcaagt acgcctggcg
     1681 gcgcttcacc tacgacttca cgccggcgcg cgccggctac accacgctcg tcgcgcgcgc
     1741 ctgggacgcc gccggcaacg tgcagccggc gatcccggcg tggaacccgc tgggctattt
     1801 ctggaacggc tggcaccgcg tgggggtggt ggttgaggcg tgagggactg gtttttcttc
     1861 ttctattggg aattcaattc tctgcagaag cgcaagacag gctgatctcc ggccctggct
     1921 ccaccctcac cgagaacaag tgcaagatct gccacgagct gcagcacatc cgccgctcgc
     1981 cgctttcgcg cggcgaatgg gccgacaaca tcaggaacat gaaggagcgc ggcacgcc
//
LOCUS       DASYJA010000007         1799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2089715, whole genome shotgun sequence.
ACCESSION   DASYJA010000007 DASYJA010000000
VERSION     DASYJA010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2089715"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1789)
                     /locus_tag="VGO02_00155"
     CDS             complement(<1..1789)
                     /locus_tag="VGO02_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476127.1"
                     /translation="MFKPADARRLAEEILNLTGADEEAESTLEQILTQTKAWPELAKL
                     LHGRARRVSDSAERVRLLFKIAQLEEEKVGDLAATAQTLEAVVDLEPSNDRALRSLIR
                     VLEARQDWPALAAALRRDFGLRATQPDREEVALRIGQLQETRIGDAGAAFTTYSEVLQ
                     ANPLSAAAVTGLERLGAAGHPNKIEIARLSLPYYQRTDNAAKLAGAFEALTGDPAATP
                     EDRLQQLQKLRTLYAGPLKDPKAAYGAALRIFAEVDPADVENRELLIHFAAETQVVAE
                     LADRMRVVANATDDQKLRRDLLVEIAQLHEQRLGRAQDAEKVYAEILQVEPLHAGAAR
                     ALGRLYRDSERWTELRALLEGRQALVTEPRERLDLLAQIAEIDEAVLDDVDHAVGSYE
                     QMLQLNPADLRAHRALDRHYATQERWKDLEALLGTRVTFASAPEIPELEFRRAEIRVA
                     RFDDVEGALHLLETIVRAAPSHEGARRLLERILALPNHRQRASKILEPLYESSGAWSR
                     LVAVLELQREGKQGKAAAALLARIADLQENKLQARTAALASWRQVLTEDPTNPDALTE
                     VERLATALERFSELVDVYQELAFSRDTADI"
BASE COUNT          235 a          659 c          610 g          295 t
ORIGIN      
        1 tgatgtccgc ggtgtcgcgg ctgaacgcca gctcctgata gacgtccacc agctcggaga
       61 agcgctccag cgcggtggcc agccgttcga cctcggtgag ggcgtcggga ttggtcggat
      121 cctcggtcag cacctgccgc cagctggcca gcgccgccgt gcgcgcctgc agcttgttct
      181 cttgcagatc ggcgatgcga gccagcagcg cggccgctgc cttgccctgc ttcccctcgc
      241 gctgcagctc cagcaccgcg accaggcgcg accaggcgcc gctgctctcg tacagcggct
      301 cgagaatctt cgacgcccgc tggcggtgat tcggcagcgc caggatccgc tccagcaacc
      361 gccgcgcgcc ctcgtggctg ggggcggcgc gcacgatcgt ctccagcagg tgaagggcac
      421 cctccacgtc gtcgaaccga gcgacgcgaa tctcggcgcg ccggaactcc agctccggga
      481 tctcgggcgc cgacgcgaag gtgacccgcg tccccagcag ggcctcgaga tccttccagc
      541 gctcctgggt cgcgtagtgc cgatccaggg cgcgatgcgc gcgcagatcg gccgggttca
      601 gctgcagcat ctgctcgtag ctgccgaccg cgtggtcgac gtcgtccagg accgcctcgt
      661 cgatctcggc tatctgcgcc agaagatcaa gccgttcgcg cggctccgtc accagcgcct
      721 ggcgaccctc caaaagcgcc cgcagttcgg tccagcgctc gctatcgcga tataggcgcc
      781 ccagcgcgcg cgcggccccg gcgtgcagcg gttcgacctg cagaatctcg gcgtagacct
      841 tctcggcatc ctgcgcgcgc ccgaggcgct gctcgtgcag ctgcgcgatc tcgaccagga
      901 gatcgcgccg caacttttgg tcgtcggtgg cgttggccac cacgcgcatg cggtcggcca
      961 gctcggcgac cacctgggtc tcggccgcaa aatggatcag gagctcacgg ttctcgacgt
     1021 cggccgggtc cacctcggca aagatgcgca gggccgcgcc gtaggcggcc ttcgggtcct
     1081 tgagcggccc ggcgtacagc gtgcgtagct tctgcagctg ctgcaacctg tcttccggcg
     1141 tggccgccgg atccccggtc agcgcctcga aggcaccggc cagcttggcg gcgttgtcgg
     1201 tgcgctggta gtagggcagc gacaggcgcg cgatctcgat cttgttgggg tgcccggccg
     1261 cgcccagccg ttccagcccg gtcacggccg ccgccgacag cgggttcgcc tgcagcacct
     1321 cgctgtaggt cgtgaaggcc gcaccggcgt cgccgatgcg cgtctcctgc agctggccga
     1381 tgcgcagcgc cacctcctcg cgatcgggct gcgtcgcgcg caggccgaag tcgcggcgta
     1441 acgccgccgc cagcgccggc cagtcctggc gcgcctcgag gacgcggatc aagctgcgaa
     1501 gggcgcgatc gttcgacggc tcgaggtcga ccaccgcctc cagcgtctgc gccgtggccg
     1561 ccagatcgcc gaccttctcc tcttcaagtt gcgcgatctt gaacagcagc cggactcttt
     1621 cggcgctgtc gctgacccgg cgcgcccgcc cgtgcagcag cttggccagc tcgggccacg
     1681 ccttggtctg ggtcaggatc tgctcgaggg tgctctcggc ctcctcgtcg gcgccggtca
     1741 ggttcaggat ctcttcggcc agccggcgcg cgtcggccgg cttgaacaag cggctggtg
//
LOCUS       DASYJA010000008         6557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1272767, whole genome shotgun sequence.
ACCESSION   DASYJA010000008 DASYJA010000000
VERSION     DASYJA010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6557
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272767"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VGO02_00160"
     CDS             complement(<1..809)
                     /locus_tag="VGO02_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV7476128.1"
                     /translation="MRRVLVTGVAGFIGMHCAQRLLARGDKVIGIDSLNDYYPVKLKR
                     DRLKQLKHKSFAFEQIDLSKEKALQKAFGKKPDAVLHLAAQAGVRYSLENPGAYVQAN
                     LVGFANLLECCRRWPPRHLVFASSSSVYGANTKLPWSEADSVDRPVSVYAATKKSNEL
                     MAHVYSHLYRLPATGLRYFTVYGPWGRPDMSPVLFARAIMEGKPIAVFNHGDMQRDFT
                     YVDDVVTGTLRVLDRPAPYAIYNIGNHTPVSLPDYIRTLERALGRKAKLKM"
     gene            complement(815..1576)
                     /locus_tag="VGO02_00165"
     CDS             complement(815..1576)
                     /locus_tag="VGO02_00165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV7476129.1"
                     /translation="MATRAALSAVIITRNAARVLPACLESVAFADETIIVDSGSSDET
                     RELAAARGVRVLQKEWLGYGRQKQFAVQQAAHDWVLCVDSDERVSPELAASIGAALAA
                     PVSPVYRMARRNRFLGRWLSHGEGYPDWSPRLFNRLEARWSEDLVHEKVLYAVTPGTL
                     RGDLMHDSFEDLSTYLERQNRYTTLAARQAYELGRGGGLLHLLLSPFVRFLKFYILRL
                     GFLDGLPGLLHISIGCMNSYMKYAKLIELRKAEQQ"
     gene            1630..2577
                     /gene="waaC"
                     /locus_tag="VGO02_00170"
     CDS             1630..2577
                     /gene="waaC"
                     /locus_tag="VGO02_00170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEV7476130.1"
                     /translation="MKRILLIRMSSLGDIIHTFPAVTDLRRALPDAALDWVVEEAYLP
                     LARMHPGVTRVLPIALRRWRRELLSARTWREATAFRNGVREPAYDAILDTQGLLKSAL
                     VAAAARGPVHGFGRGTARETLATRFYDVTYEFAAADHKVERYRSVAARALGYVPGALD
                     YGLNAPPRPDGIPFSPYCVLLHSTARAAKLWHDADWIEVGRHLAARGLVCVLPWGEDE
                     ERLRAERLATAIPRAWVPAHMPLADACGLIGHAKLVIGVDTGLMHLAAALRVPVLGIF
                     CDSEPLDAHPLGTGRVAFRGGIGKPPSAQEVIAALAELA"
     gene            2631..3812
                     /locus_tag="VGO02_00175"
     CDS             2631..3812
                     /locus_tag="VGO02_00175"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEV7476131.1"
                     /translation="MWWRGRREPGYRRGIAERFGSYAVAGGAKLIWVHAVSVGEARAA
                     APLVEALRAGFPDHAVLLTCTTAAGRDTLRQIHGAGALVTYLPYDLPGAVRGFLGHWH
                     PRLGVLMETEVWPNLLAACAEAGVPVVLANARMSEKSARGYARWPALGRAMFASLAEV
                     CAQSAADAERLRALGARNVAVTGNLKFDVEPDAAQLAAGRAWRARLGRPVLLLASTRE
                     GEEQLLLKEASQMKDLPLIVIVPRHPQRFDEVAQWADARRSRQDVPDGKTKVYLGDTM
                     GEMAFYFAACDVAVIGGSFLPLGGQNLIEALAAGAPVVTGPSMFNFAEATRLALETRA
                     AIQAADAPSALREAVRLLGDPARRAQMAAAGKRLCEAHRGATARHLAVCLRLLPGAAR
                     G"
     gene            complement(3754..5118)
                     /locus_tag="VGO02_00180"
     CDS             complement(3754..5118)
                     /locus_tag="VGO02_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEV7476132.1"
                     /translation="MKTTKILAFALLGAAQPAAAEDLLQVYRDSQRYDATYSAARNTL
                     AAGRERLPQGRALLLPTLNLSASATRQRLDVESRDPALSPSFIRSPESNGYTLTLTQP
                     IFRAQNWMQYRQGEYQVRQAEATFGQASQDLIVRVAQAYFDVLGQYDTLVLVRAQKAA
                     ISEQLAQAKRNFEVGTATITDTHEAQARYDLSAAQEIAAQNDLQARRRQLQLIAGKEY
                     TELKQLRSDIKLAPPNPENVDTWADFADKQSYPVLIGEAAAEVAALEARRASAGHLPT
                     LDFVATYGQTAESATATSPIGRDTTASTVGVQLALPIFQGGSVSSREREAAALYQRSR
                     DDLDNARRTAVLTARQLYLSTLNGITQVTALEQAKVSSQSALDSNKLGYEVGIRINID
                     VLNAQQQLYSTERDLALARYNTILNNLRLKASAGTLREEDLEEINRALRQEGGASRPP
                     DGAR"
     gene            complement(5122..5445)
                     /locus_tag="VGO02_00185"
     CDS             complement(5122..5445)
                     /locus_tag="VGO02_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV7476133.1"
                     /translation="MKQITPAELAGWLGEKARARPLLLDVREAWEFQTCSIAGSRHVP
                     MGEVAARRDELDPRADIVVICHHGGRSMQVALFLEKSGFGKVHNLAGGVDSWARTVEP
                     SMPVY"
     gene            complement(5462..6115)
                     /locus_tag="VGO02_00190"
     CDS             complement(5462..6115)
                     /locus_tag="VGO02_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEV7476134.1"
                     /translation="MNIEQARSNMVEQQIRTWEVLDQEVLDLLYLIPREEFVAQEHRA
                     LAFSDLELPIGEGERMWTPKMEARVLQELAPKKQDLVLEVGTGSGYLTALLAHRAGHV
                     HSVEVKPKLAALGHRNLARLGVDNVTLHTGDASHGWPRQAPYDVIVLTGSTPLLPSAL
                     LDSLASGGRLFAVVGEAPAMAARLVTCTAPGARRSVDLFETVIAPLVNAEHPPRFQF"
     regulatory      6136..6228
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6217..>6557
                     /locus_tag="VGO02_00195"
     CDS             6217..>6557
                     /locus_tag="VGO02_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878158.1"
                     /note="catalyzes the formation of
                     4-amino-2-methyl-5-phosphomethylpyrimidine from
                     5-amino-1-(5-phospho-D-ribosyl)imidazole and
                     S-adenosyl-L-methionine in thiamine biosynthesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEV7476135.1"
                     /translation="MNANPKFLAATAHVDDAAIKPLPNSRKVYVGELKVPMREVSQAD
                     TPSMFGGEKNPPIYVYDCSGPYTDPAAKIDIRSGLPALREPWIVSRNDSEILSGPSSA
                     YGKERLADATLA"
BASE COUNT          937 a         2206 c         2317 g         1097 t
ORIGIN      
        1 ttcattttca acttcgcttt tctgccgagg gcgcgctcga gcgtcctgat gtagtccggc
       61 agcgagaccg gcgtatggtt gccgatgttg tagatcgcat agggcgcggg ccggtcgagc
      121 acgcgcaggg tgccggtcac gacgtcgtcg acgtaggtga agtcgcgctg catgtcgccg
      181 tggttgaaca ccgcgatcgg cttgccctcc atgatggcgc gcgcgaacag gaccggcgac
      241 atgtcgggcc ggccccaggg accgtagacc gtgaaatagc gcaggccggt cgccggcagg
      301 cggtacaggt ggctgtagac gtgcgccatc agctcgttcg atttcttcgt ggcggcgtag
      361 acgctgaccg gccggtcgac gctgtcggct tccgaccagg gcagcttggt gttggcgccg
      421 tagacgctcg agctcgaggc gaacacgaga tggcgcggcg gccaccggcg gcagcactcg
      481 agcaggttgg cgaagccgac caggttggcc tggacgtacg cgccgggatt ctcgagcgag
      541 tagcgcacgc cggcctgcgc ggcgaggtgc agcacggcat cgggcttttt gccgaatgcc
      601 ttttgcaagg ctttctcctt cgagaggtcg atctgctcga aggcaaagct cttgtgcttg
      661 agctgcttca ggcggtcgcg cttcagcttg accgggtagt agtcgttcag gctgtcgatg
      721 ccgatcacct tgtcgccgcg cgcgagcaac cgctgcgcgc agtgcatccc aatgaagccg
      781 gcaacgccgg taaccaggac tcgtctcatt ttccctattg ttgctcggcc ttgcgcagct
      841 cgatcagctt ggcgtacttc atgtagctgt tcatgcagcc gatggagatg tgcagcagcc
      901 ccggcaggcc gtcgaggaaa ccgaggcgca ggatgtagaa cttgagaaag cgcacgaacg
      961 gcgacaggag caggtgcagc aatccgccgc cgcggccgag ctcgtaggcc tggcgcgcgg
     1021 cgagcgtggt gtagcggttc tggcgctcca ggtacgtgct caggtcttcg aacgagtcgt
     1081 gcatcaggtc gccgcgcaac gtgcccggcg tgaccgcgta cagaactttc tcgtgcacca
     1141 ggtcctcgct ccagcgcgcc tcaagccggt tgaaaaggcg cgggctccag tcgggatagc
     1201 cttcgccgtg cgacagccag cggccgagga agcggttgcg ccgcgccatg cggtaaacgg
     1261 gcgacaccgg cgccgccagg gcggcgccaa tgctcgcggc gagctcgggg gagacgcgct
     1321 cgtcggagtc cacgcacagc acccagtcgt gcgccgcctg ctgcaccgcg aactgctttt
     1381 gccgcccgta gcccagccat tccttctgca gcacgcgtac gccgcgcgcg gcggccagct
     1441 cgcgcgtctc gtcgctcgag ccactgtcga cgataatggt ctcgtcggcg aaggcaacgc
     1501 tttccaggca cgcggggagg acgcgcgccg cattgcgggt gatgatgacg gccgagagcg
     1561 ctgcgcgcgt agccaactcg gttctcttat aaaatccggc aggcgtgacc agcggatcat
     1621 tctagcgcgg tgaagcggat actgctcatc cgcatgtcct cgctggggga catcatccac
     1681 acctttcccg ccgtcaccga cttgcgccgc gcgctgccag acgccgcgct cgactgggtg
     1741 gtcgaggagg cctatttgcc gctcgcgcgg atgcacccgg gcgtcacgcg agtcctgccc
     1801 atcgcgctgc gccgctggcg ccgcgagctc ctttcggcgc gcacctggcg cgaggcgacg
     1861 gcgtttcgca atggcgtgcg cgagcctgcc tacgacgcca ttctcgacac gcagggcctg
     1921 ctgaagagcg cgctggtggc cgcggctgcg cgcggcccgg tgcacggctt cggccgcggc
     1981 acggcgcgcg agacgctcgc cacgcgcttc tacgacgtga catacgaatt cgccgccgcc
     2041 gaccacaagg tcgagcgcta ccgctcggtg gcggcgcggg cgctcggcta tgtgcccggc
     2101 gcgctggact atggcctgaa cgcgccgccg cgaccggatg gcattccgtt ttctccctat
     2161 tgcgtgctgc tgcactcgac ggcccgcgcg gcgaagctct ggcacgacgc cgactggatc
     2221 gaggtggggc ggcatctcgc cgcgcgcggc ctcgtctgcg tgctgccttg gggcgaagac
     2281 gaagagcgct tgcgggccga acggctcgcc accgccatcc cgcgcgcgtg ggtgcccgcg
     2341 cacatgccgc tggccgacgc atgcggcctg atcggccatg ccaagctggt catcggggtc
     2401 gataccgggc tgatgcacct cgcggcggcc ttgcgcgtcc cggtgcttgg catcttctgc
     2461 gattccgagc cgctcgacgc ccacccgctg ggcacgggac gcgttgcgtt ccgcggcggc
     2521 atcggcaagc cgccgagcgc gcaggaagtc atcgccgccc tggcggagct cgcctgagga
     2581 caatctatac ctggctcctg cgcgcggccc tgccgctgat cctgctgcgc ctgtggtggc
     2641 gcgggcgccg cgagccgggc tatcgccgcg gcatcgccga gcgcttcggc agctacgcgg
     2701 ttgccggcgg cgcaaagctc atctgggtgc acgccgtatc ggtgggagag gcgcgcgccg
     2761 cggcgccgct ggtggaggcg ttgcgcgccg gatttccgga tcatgccgtg ctcctcacct
     2821 gcaccaccgc tgccggccgc gacaccctgc gccagatcca cggcgccggc gcgctggtga
     2881 cctatcttcc ttacgacctg ccgggcgcgg tgcgcggctt cctcggacac tggcatccgc
     2941 gcctgggggt gctgatggaa accgaggtat ggccgaacct gctcgcggcc tgcgccgagg
     3001 ccggggtgcc ggtcgtgctg gcgaacgcgc gcatgtcgga aaagtcggcg cgcggctatg
     3061 cgcgctggcc ggcgctcggc cgcgcgatgt tcgccagcct ggccgaggtc tgcgcgcaga
     3121 gcgcggccga cgcggagcgc ctgcgcgcgc tcggcgcgcg caacgtcgcg gttaccggca
     3181 atctcaagtt cgacgtcgag cccgacgcgg cccagctcgc ggccgggcgc gcctggcgcg
     3241 cgcgcctcgg ccgtccggtc ctgctattgg cgagcacccg cgagggcgaa gagcagctgc
     3301 tcttgaagga agccagccag atgaaagacc tgccgctgat cgtcatcgtg ccccggcacc
     3361 cgcagcgctt cgacgaggtc gcgcagtggg ccgacgcgcg gcgcagccgg caggacgttc
     3421 cggacggcaa gacgaaggtc tacctgggcg acacgatggg cgagatggcg ttctatttcg
     3481 ccgcctgcga cgtcgccgtg atcggcggct cgttcctgcc gctcggcggg cagaacctga
     3541 tcgaggccct tgccgccggc gcgccggtgg tgaccggccc gagcatgttc aacttcgccg
     3601 aggcgacgcg cctcgcgctc gagacgcgcg cggcgatcca ggcggccgac gcgccttcgg
     3661 cgctgcggga agcggtgcgg ctgctcggcg atccggcgcg ccgcgcgcag atggcggcgg
     3721 cgggcaagcg gctgtgcgag gcgcaccgcg gcgctaccgc gcgccatctg gcggtctgct
     3781 tgcgcctcct tcctggcgca gcgcgcggtt gatttcctcg aggtcctctt cgcgcagggt
     3841 tccggcggag gccttgaggc gcaggttgtt gaggatggtg ttgtagcgcg ccagcgcgag
     3901 gtcgcgctcg gtggagtaga gctgctgctg cgcgttcagc acgtcgatat tgatgcggat
     3961 gccgacctcg tagccgagct tgttcgagtc gagcgcgctc tgcgaggata ccttggcctg
     4021 ctcgagcgcc gtgacctggg tgatgccgtt gagcgtcgag aggtagagct ggcgcgcggt
     4081 gagcaccgcg gtgcggcgcg cgttgtccag atcgtctctc gagcgctgat agagcgcagc
     4141 cgcctcgcgc tcgcgcgagg agacgctgcc gccctggaag atcggcagcg cgagctgcac
     4201 gccgacggtc gaggcggtgg tgtcgcggcc gatcggactg gtggcggtcg cgctttccgc
     4261 ggtctgcccg taggtggcga cgaagtcgag ggtcggcagg tggccggccg aggcgcgcct
     4321 cgcctcgagc gcggcgactt cggcggcggc ctccccgatc agcaccgggt agctctgctt
     4381 atcggcgaaa tcggcccacg tgtcgacgtt ctccgggttc ggcggcgcga gcttgatgtc
     4441 gctgcgtagc tgcttgagct cggtgtactc cttgccggcg atgagctgca gctggcggcg
     4501 gcgcgcctgc aggtcgttct gcgccgcgat ctcctgcgcc gcgctcaggt cgtagcgcgc
     4561 ctgcgcctcg tgcgtgtcgg tgatggtcgc cgtgccgacc tcgaagttgc gcttggcctg
     4621 cgccagctgc tcggagatgg ccgctttctg cgcgcgcacc aggaccagcg tgtcgtactg
     4681 gccgaggacg tcgaagtacg cctgcgcgac gcgcacgatg aggtcctggc tcgcctggcc
     4741 gaaggtggcc tctgcctggc gcacctggta ttcgccctgc cggtactgca tccagttctg
     4801 cgcgcggaag atcggctgcg tcaaggtgag cgtatagccg ttcgactcgg ggctgcgaat
     4861 gaagctcggc gagagcgccg ggtccctcga ttcgacgtcg aggcgctggc gcgtcgcgct
     4921 cgccgacagg ttgagcgtcg gcaggagcag tgcgcgcccc tgcggcagcc gctcgcgtcc
     4981 cgccgccagc gtattgcgcg ccgccgagta ggtggcgtcg tagcgttgcg agtcgcggta
     5041 cacctgcagc agatcctcgg ctgccgcggg ctgcgccgcg ccgaggagcg caaaggccag
     5101 tatcttggtt gtcttcattc ttcagtacac cggcatcgag ggctcgaccg tccgggccca
     5161 ggaatccacg ccccctgcga gattgtgcac cttgccgaat ccgctttttt cgagaaacag
     5221 cgccacctgc atgctgcgcc cgccatgatg gcagatgacg acgatgtccg cccgaggatc
     5281 gagctcgtca cggcgcgcgg ctacctcgcc catcggcaca tgccgcgagc cggcgatgct
     5341 gcaagtctgg aattcccacg cctcgcgcac atcgagcagc agcggcctgg cgcgtgcctt
     5401 ttcgccgagc cagccggcaa gctccgcggg cgtgatctgc ttcatcgacg cgcgagccgc
     5461 ctcaaaactg aaatcgcggc gggtgctccg cgtttaccag gggcgcgatc acggtctcga
     5521 acagatcgac gctgcgacgc gcaccggggg cggtgcaggt gacgaggcgc gccgccatgg
     5581 ccggcgcctc gccgaccact gcgaacagcc gcccgccgct cgccaggctg tcgagcaggg
     5641 cgctcggcag cagcggcgtc gagccggtga gcacgatgac gtcgtagggc gcctggcgcg
     5701 gccagccgtg cgaggcgtcg ccggtgtgca gcgtcacgtt atcgacgccc aggcgcgcga
     5761 ggttgcggtg gccgagggcg gcgagcttcg gcttgacctc gacggaatgc acgtggccgg
     5821 cgcggtgcgc gagcagggcc gtcaggtagc cgctgccggt gccgacctcg agcaccagat
     5881 cctgcttctt cggcgccagc tcctgcagca cgcgagcttc catcttcggc gtccacatgc
     5941 gttccccttc cccaatcggc agctcgaggt cggagaaggc cagcgcgcga tgctcctggg
     6001 cgacaaactc ctcgcgcggg atgagataca gcaggtcgag cacttcctga tcgagcacct
     6061 cccaggtgcg gatctgctgc tcgaccatgt tgctgcgggc ctgctctatg ttcatagcga
     6121 gcgggattat aatctcgcct tgcgtagggg tcccgcgaga gtgggtgaga gagtccctta
     6181 gaacctgatg cgggtcatcc cgccgtagga aacgccatga acgccaatcc caagtttctc
     6241 gccgccaccg cccacgtaga cgacgcggcg atcaagccgc tgcccaactc gcgcaaggtc
     6301 tacgtcggcg agctcaaggt gccgatgcgc gaggtcagcc aggcggatac gccgagcatg
     6361 ttcggcggcg agaagaaccc gccgatctac gtctacgact gctccggccc gtacaccgac
     6421 ccggcggcga aaatcgacat ccgctccggc ctgccggctc tgcgcgagcc atggatcgtc
     6481 tccaggaacg attcggagat tttgagcggg ccttcttccg cttatgggaa ggaaaggctg
     6541 gccgatgcca cgctggc
//
LOCUS       DASYJA010000009         1970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1726778, whole genome shotgun sequence.
ACCESSION   DASYJA010000009 DASYJA010000000
VERSION     DASYJA010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1726778"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..982
                     /locus_tag="VGO02_00200"
     CDS             167..982
                     /locus_tag="VGO02_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7476136.1"
                     /translation="MKLKAPKGATDTHMHFYDGALSAAPGTPNPGHFTVPMYRELQER
                     LGLERVIVVQPNAYRDDNRVTLEAMKAIGRNAKGVAVVKPGTPQAELERLSKAGICAV
                     RIMTLHGGMLGFDVMDAVMAQVHPFGWHANIQLDGRELPKFEAQIKRLPGKFVIDHTG
                     KFLEPVPPDSREFRSLLSLVDSGRCWVKLSAPYETSKTGAPKYEDVSRLARALVKQAP
                     ERMLWASNWPHPSVPKEQRPSDADLLDLLLDWAPDEKLRRKILVDNPAELYGY"
     gene            972..1961
                     /locus_tag="VGO02_00205"
     CDS             972..1961
                     /locus_tag="VGO02_00205"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV7476137.1"
                     /translation="MVTRLAIVGLGMAVTPHAKSLLDLKGRVQVAYAFSPSAERRQKF
                     AERFPFRQCDRIETILQDSSVDAVLILTPPNTHLELAERCAAAGKHVLLEKPLEISTA
                     RAERLVEIFSEKKLKLGIVLQHRFRPAAERLREVLPELGRIVSASAMIPNWRPQSYYD
                     QPGRGTRARDGGGVLLTQGIHTLDLFLSFAGEAEEVRSFTATTPVHRMETEDLACAAV
                     RFKSGALGVVHATTAAYPGFAERIELIGTEGTALLEGTFLQVDLHDKPSIQFSTDAGG
                     GGTGADPMAFPHDWHRSLLADFLYAIEKNLQPRINGAEGLKVHRFIDQLLQTR"
BASE COUNT          362 a          648 c          649 g          311 t
ORIGIN      
        1 ccgcgcgcct cggctatcgc ccgcaggaca acgcggagac ctatgccgcg gagattctcg
       61 ccaaggaaaa gcccggcgat ccgttcgtcg agacctacca gggcggggtc ttcgtcaagg
      121 tcgaggcggt cgcgaacccg gcggctgaaa ggcagggagc gaagaaatga agctcaaggc
      181 tcccaagggc gcgaccgaca cgcacatgca tttctacgac ggcgcgttga gcgcagcgcc
      241 cggcacgccg aatcccggcc actttacggt gccgatgtac cgggagctgc aggaacgcct
      301 gggcctcgag cgggtgatcg tggtccagcc caacgcctac cgcgacgaca accgcgttac
      361 cctcgaggcg atgaaggcga tcggcaggaa cgccaaaggc gtcgcggtgg tgaaaccggg
      421 tacgccgcag gcggagctcg agcggctgag caaggcgggc atctgcgcgg tgcgcatcat
      481 gacgctgcac ggcggcatgc tcggcttcga cgtgatggac gcggtgatgg ctcaggttca
      541 cccgttcggc tggcacgcga acatccagct cgacggccgc gagctgccga agttcgaggc
      601 gcagatcaag cgcctgcccg gcaagttcgt catcgaccac accggaaaat ttctggaacc
      661 cgtcccgcct gattccaggg aatttcgttc cttgttgagt cttgtcgata gcggccgttg
      721 ctgggtcaag ctgtcggcgc cctacgagac ttccaagacc ggcgcgccga agtacgagga
      781 cgtcagccgg cttgccaggg cgctggtgaa gcaggcgccc gagcgcatgc tgtgggcgtc
      841 gaactggccg cacccctcgg tgccgaagga acagcgtccg tcggacgcgg acctgctcga
      901 tctgctgctc gactgggcgc ccgacgagaa gctgcgcagg aagatcctgg tcgacaatcc
      961 cgccgagctc tatggttact aggttggcga tcgtcgggct cggcatggcg gtcacgccgc
     1021 atgccaagag cctgctcgat ctcaagggcc gggtgcaggt cgcctacgcc ttcagcccga
     1081 gcgccgagcg gcgccagaaa ttcgccgagc gcttcccgtt ccgccagtgc gaccgcatcg
     1141 agacgatcct gcaggacagc tcggtcgacg cggtcctgat cctgaccccg ccgaataccc
     1201 acctcgagct ggccgaaagg tgcgctgccg ccggcaagca tgtcctcctg gaaaagcctc
     1261 tggaaatttc gactgcgcgc gccgagcgtc tggtcgaaat cttctctgaa aaaaaactca
     1321 agctgggaat cgtcctgcag catcgcttca ggccggcggc cgagaggctg cgcgaagttc
     1381 ttccggagct cggcaggatc gtctccgcct cggcgatgat cccgaactgg cggccgcaga
     1441 gctactacga ccagcctggg cgcggcactc gcgcgcgcga cggcggcggc gtgctcctga
     1501 cgcagggcat ccatacgctg gacctgttcc tgtcgtttgc gggcgaagcc gaagaggtga
     1561 ggagcttcac cgccacgacg ccggttcatc gcatggagac ggaagatctc gcttgcgcgg
     1621 ccgtgcgctt caagagcggt gcgctgggcg tggtccacgc caccaccgcg gcctatcccg
     1681 gctttgcgga aaggatcgag ctgatcggca ccgagggcac cgcgctactc gagggaacgt
     1741 tcctccaggt cgatctgcac gacaagcctt cgatccagtt ctcgaccgat gccggcggcg
     1801 ggggcacggg cgccgacccg atggccttcc cgcacgactg gcaccgcagc ctgctggcgg
     1861 acttcctgta cgcaatcgag aagaacctcc agccgcgcat caacggcgcc gaagggctga
     1921 aggtccaccg cttcatcgac cagctgctgc aaactcgctg atcaggcggt
//
LOCUS       DASYJA010000010         4934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_456160, whole genome shotgun sequence.
ACCESSION   DASYJA010000010 DASYJA010000000
VERSION     DASYJA010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456160"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..979)
                     /locus_tag="VGO02_00210"
     CDS             complement(14..979)
                     /locus_tag="VGO02_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476138.1"
                     /translation="MRVLAFLLAFLSFQAGAQTPAQQIQATSPQLVVFAGSDANLQSL
                     ASGLSLGQTVTLVTPGADGLLQIVTFTPPSALGTGVASALEQARTNLIARGIAQPTAQ
                     QIAVALMGGTIATSAGQVTAVTGVLTGSTNTNAVAVRNEFAANFANGLTPFGGSATNF
                     QALSTGLRQGTPITLTGVVNGVTQTVVFTPPGGAMSAADANQLLQLANQSLLSLGIIN
                     PTPQQIQIALLGGTVPVAGGSVLLQGVLTNRAVNTSTSSIIGTSNSPIVSSPTIGSPA
                     VSSGAGASSAPIPEKTQVIAASTATPAARSTITALVAASRPTRPP"
     gene            complement(1024..1989)
                     /locus_tag="VGO02_00215"
     CDS             complement(1024..1989)
                     /locus_tag="VGO02_00215"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7476139.1"
                     /translation="MKVVALEEHYWDAEVASHFKERGPEMRNPQMLERLHDLGARRLK
                     EMDEAGIDLQVLSHGAPATQRLDGDLAIQVARGANDRLFESIKKHPGRFEAFAVLPSN
                     EPKAAADELERAVTRLGFKGAMLHGLAYGEKNSGLFLDDKRFWPIYERAQKLDVPIYL
                     HPAVPHPAVVEAYYKDYLKEFPGLLTAAWGFTVETATQGIRMVLSGVFDAYPGLKMIL
                     GHLGESLPFSLWRIDMALQRPGNAPTPFRDTFRNHFWITTSGNFSTPALLCCVMEMGA
                     DRILFSVDYPFVPNPPGVKWMETVPLGPEDRAKILHGNATRLLKL"
     gene            complement(1986..2564)
                     /locus_tag="VGO02_00220"
     CDS             complement(1986..2564)
                     /locus_tag="VGO02_00220"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV7476140.1"
                     /translation="MPVTQSLLVANVLAFLLLEGSGLPVVEWFALWPPSDPRFEAWQI
                     VTYSFLHGGVAHIVFNMIALYTFGAAIERLFGSRFYLLYYFACVVSAAICHLIVTGLM
                     GGPPVPTVGASGGIYGLLLAYGLYFPHQRVMLLIPPIPMNARTLVFAFAAIELFLGVT
                     GTAAGIAHFAHLGGMLGGWLMIEYRKRGGGRL"
     gene            complement(2596..3387)
                     /locus_tag="VGO02_00225"
     CDS             complement(2596..3387)
                     /locus_tag="VGO02_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEV7476141.1"
                     /translation="MDVQGHRGARGLLPENTLPAFQRALELGVDTLELDCGVTKDGVV
                     VVHHDRRLNPDVARGPDGKWVSSPGPTIRSLTYAELQRYDVGRLRSGSEYQARFPQQQ
                     AMDGTRIPRLEDVLKLKSKFNIETKLIPGAAEETVGPDEFARALIKEIRKAKAKATIQ
                     SFDWRTLKIVEREAPEIPTVYLTESQDADPQKVKDAGGRIWSPSFKDLTQRKVSIASS
                     LGLKIVAWTVNEPADIAAAIDIGLDGIISDYPDRVLAELKRRGQR"
     gene            complement(3422..4285)
                     /locus_tag="VGO02_00230"
     CDS             complement(3422..4285)
                     /locus_tag="VGO02_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV7476142.1"
                     /translation="MNGVAAAVVSSALGGTAAALTRYVIGASDPVTLAAFRFGIGFLL
                     IFPLALALRARWPQGRDWIGVALLGIAFFALFFFLYNIAMGYTSAARGSLALATLPLT
                     TMLLAAFLGRESLSRRKFAGVLTAVSGVVIALWGGLADAPPGAWRGDLVMLGATVVMA
                     LYSIWSRPFMARSSMLGFLAAGMGFGALASVALSFFTEGYLLVEKLNSQQWLAILCLG
                     VFGGAAAFYLWVYALERTTPTRVTNTMTVNPVSASLLAAVLVGEPLGLPLALGIVAVG
                     AGIWLASSTPR"
     gene            complement(4282..4647)
                     /locus_tag="VGO02_00235"
     CDS             complement(4282..4647)
                     /locus_tag="VGO02_00235"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV7476143.1"
                     /translation="MEEWNKGLMAHLGIRIIEATPERVVGEIEVRDELKTTTNAVHGG
                     TLMALADTVGAIGTAALGFKTATLESKTNFFAAATSGKIRAEATPLHKGKRSHVWQTR
                     VTDESGRLLSLTIQTQMIL"
     gene            complement(4631..>4934)
                     /locus_tag="VGO02_00240"
     CDS             complement(4631..>4934)
                     /locus_tag="VGO02_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476144.1"
                     /translation="VSTSYALALALLFAAGFLELSFNAMAQTLVQIRAPEALRGRVIG
                     LFAMSSLGLRAFSGVTVGLGGAAIGIHWSLALSAMALLGLTMVLFIKIGSNNGRVE"
BASE COUNT          814 a         1587 c         1736 g          797 t
ORIGIN      
        1 accgcatcgt cgcctacggc ggacgcgtcg gcctgcttgc ggcgaccaag gcggtaatcg
       61 tcgaccgcgc cgccggcgtg gcggtgctcg cggcgatgac ctgcgtcttc tccggaatcg
      121 gcgcgctgct cgcgccggcg ccggagctga cggcggggct gccgatggtc gggctgctca
      181 cgatcgggct gttgctcgtg ccgatgatgc tgctggtgct ggtgttgacg gcacgattgg
      241 tcagcacgcc ctgcaacagg acgctaccgc cggccaccgg caccgtgccg ccgaggagcg
      301 cgatctggat ctgctgcggc gtcggattga tgatgcccag gctgaggagc gactggttgg
      361 cgagctgcag cagctggttg gcgtcggccg cgctcatcgc gccgcccggc ggcgtgaaga
      421 ccaccgtctg cgtcacgcca ttcaccacgc cggtgagcgt gatcggcgtg ccctggcgca
      481 gtccggtgct gagcgcctgg aagttggttg ccgagccgcc gaagggcgtc aggccgttgg
      541 cgaagttggc ggcaaattcg ttgcgcacgg cgactgcgtt ggtgttggtg ctgccggtaa
      601 ggacgcccgt taccgcggtc acctgccccg cactcgtggc gatcgtgccg cccatcagcg
      661 ccaccgcgat ttgctgcgcg gtgggctgtg cgatgccgcg cgcgatgagg ttcgtgcgcg
      721 cctgctcgag cgcgctcgcc acgcccgtgc cgagcgcgct gggcggcgtg aaggtgacga
      781 tctgcagcag gccgtcggcc ccgggggtca ccagcgtcac cgtctggccg agcgacagcc
      841 cgctggcgag cgactgcagg ttggcgtcgg agccggcgaa caccacgagc tgcggcgatg
      901 tggcctggat ctgctgcgcc ggggtctggg cgccggcctg gaaggacagg aaagcgagga
      961 gaaaagcgag gacgcgcatg gtcaacctcc gatggttgcc gtgcactttt cgtgcccgga
     1021 ctcctagagc ttaagcagcc tcgtcgcgtt gccgtgcagg atcttggcgc ggtcctctgg
     1081 tccgagcggc acagtctcca tccacttcac gcccggcggg ttgggcacga acggatagtc
     1141 caccgagaac aggatgcggt cggcgcccat ctccatcacg cagcagagca gcgccggggt
     1201 ggagaagttg ccgctggtgg tgatccagaa gtggttgcgg aaagtgtcgc ggaacggcgt
     1261 cggggcgttg cccggccgct gcagcgccat gtcgatgcgc cagagcgaga agggcagcga
     1321 ttcgcccagg tggccgagga tcatcttcag gccggggtag gcgtcgaaca cgcccgacag
     1381 caccatgcgg atgccttgcg tcgcggtctc gacggtgaag ccccacgcag cggtgagcag
     1441 gccggggaac tccttcaggt agtccttgta gtaggcctcg accaccgccg ggtgcggcac
     1501 ggccgggtgc aggtagatcg gcacgtcgag cttctgcgcg cgctcgtaga tcggccagaa
     1561 gcgcttgtcg tcgaggaaca ggcctgaatt tttttccccg taggccaaac cgtgcagcat
     1621 cgcccccttg aagccgagcc tggtcaccgc gcgctcgagc tcgtcggcgg ccgctttggg
     1681 ttcattgctg gggaggaccg caaacgcctc gaatcgtcca ggatgtttct tgattgattc
     1741 gaacaatctg tcgttggccc cccgcgcgac ctgaatggcc aggtctccat cgaggcgttg
     1801 cgtcgccgga gcgccgtggg aaagcacctg caggtcgatg cccgcctcgt ccatctcctt
     1861 caggcgccgc gcgccgaggt cgtgcagccg ctcgagcatc tgcgggttgc gcatctccgg
     1921 gccgcgctcc ttgaaatggc tggcgacctc cgcgtcccag tagtgctcct cgagcgccac
     1981 caccttcata gtcttccgcc ccctcgcttg cgatactcga tcatcagcca gccgccgagc
     2041 atgccgccca ggtgggcgaa gtgcgcgatg ccggcggcgg tcccggtgac gccgagaaat
     2101 aactcgatcg cggcgaacgc gaagaccagc gtgcgcgcgt tcatcgggat cggcgggatg
     2161 agcagcatga cccgctggtg cggaaagtag aggccgtagg cgagaagcag gccgtagatg
     2221 ccgcccgagg cgccgactgt gggcaccggc ggcccgccca tcaggccggt gacgatcagg
     2281 tggcagatcg cggccgagac gacgcaggcg aagtagtaca ggaggtaaaa gcgcgagccg
     2341 aagagccgct cgatggccgc gccgaaggtg tagagcgcga tcatgttgaa gacgatgtgc
     2401 gcgacgccgc cgtgcaggaa gctgtaggtg acgatctgcc aggcctcgaa gcgcgggtcc
     2461 gagggcggcc agagcgcgaa ccactcgacg acgggcagcc cgcttccctc gaggagcagg
     2521 aaggcgagca cgtttgcgac cagcagggac tgggtgaccg gcatggctaa ttgtggcttg
     2581 gtggtgccgc gcgcgctagc gctggccgcg ccgtttcaac tccgcgagca cgcggtcggg
     2641 atagtcgctg atgatgccgt ccaggccgat gtcgatggct gcggcgatgt ctgccggctc
     2701 gttgacggtc caggcgacga tcttcaggcc caggctgctc gcgatcgaga ccttgcgctg
     2761 cgtcaggtcc ttgaagctcg gcgaccagat cctgccgccg gcgtccttga ccttctgcgg
     2821 gtcggcgtcc tggctctcgg tgagatagac cgtcgggatc tcgggcgcct cgcgctcgac
     2881 gatcttcagc gtgcgccagt cgaacgactg gatggtcgcc tttgcctttg cttttcgtat
     2941 ctctttgatc aacgctcgcg cgaactcgtc cggccctacg gtctcttcgg ccgcgccggg
     3001 gatgagcttg gtttcgatgt tgaatttcga cttcaacttc aaaacatcct ccaaccgtgg
     3061 aatacgcgtg ccgtccattg cctgctgctg cgggaagcgc gcctggtatt cggagccgga
     3121 ccgcaggcgg ccgacgtcgt agcgctgcag ctcggcgtaa gtgagcgagc ggatcgtcgg
     3181 gccgggtgag ctcacccact tgccgtcggg gccgcgcgcg acgtcgggat tgagccggcg
     3241 gtcgtggtgc accacgacca ccccgtcctt ggtcacgccg cagtcgagct ccagcgtgtc
     3301 gacgccgagc tcgagcgcgc gctggaacgc cggcagcgtg ttctccggca gcaggccgcg
     3361 ggcgccgcga tgtccttgca catccattgc ctgtaatggt cctacaaaaa gagcgccagc
     3421 gttagcgcgg cgtggaggag gcgagccata ttccggcgcc gaccgcgacg atgcccaggg
     3481 cgagcggcag gccgagcggc tcgccgacga gcaccgccgc gagcagcgaa gctgacacgg
     3541 ggttgacggt catggtgttg gtgacgcggg tcggcgtggt gcgctcgagc gcgtagaccc
     3601 agagatagaa cgccgccgcg ccgccgaata ctcccaggca cagtatggcg agccattgct
     3661 gcgaatttag cttctcaacc agcaaataac cttctgtgaa aaaagaaagc gccacgctcg
     3721 ccagcgcgcc gaaacccatc ccggcggcga ggaagccgag catgctggag cgcgccatga
     3781 acggccgcga ccagatgctg tagagcgcca tgacgacggt cgcgccgagc atcaccaggt
     3841 cgccgcgcca cgcgcccggc ggcgcgtcag ccagccctcc ccagagcgca atgacgacgc
     3901 cgctcaccgc cgtgagcact ccggcgaatt ttcttctgga gagactttcc ctgccgagaa
     3961 acgcagccag cagcatggtc gtcaagggca gggtggcaag agcgagcgag ccgcgcgccg
     4021 cgctggtgta gcccatggcg atgttgtaca ggaagaagaa cagcgcgaag aaggcgatgc
     4081 cgaggagggc gacgccgatc cagtcgcggc cctgcggcca gcgtgcgcgc agcgcgagcg
     4141 ccagggggaa gatcagcagg aagccgatgc cgaagcgaaa ggcggcgagg gtcaccgggt
     4201 cggaggcgcc gatgacgtag cgtgtcagcg ccgcagcggt gccgccgagc gcgctcgaca
     4261 ccaccgccgc agccacgccg ttcacaggat catctgggtc tggatggtga gcgagagaag
     4321 gcggcccgac tcgtcggtga cgcgcgtctg ccagacgtgc gagcgcttgc ccttgtgcag
     4381 cggcgttgcc tccgcgcgga tcttgccgct ggtggcggca gcgaagaagt tggtcttcga
     4441 ctcgagggtc gcggtcttga agccgagcgc cgcagtgccg atcgcgccga ccgtgtcggc
     4501 cagcgccatc agcgtgccgc cgtgcaccgc gttggtggtg gtcttcaatt catcgcgcac
     4561 ctcgatctcg ccgaccacgc gctcgggcgt tgcctcgatg atgcggattc ccagatgggc
     4621 catcaggccc ttattccact cttccattgt tcgatcctat cttgatgaac agaaccattg
     4681 tcagcccaag cagcgccatc gcactgagcg ccagggacca atggatgccg atcgcggcgc
     4741 cgcccaggcc gacggtgacg ccgctgaagg cgcgcaggcc gaggctcgac atggcgaaca
     4801 ggccgatgac gcggccgcgc agcgcctcgg gcgcgcggat ctgcaccagc gtctgcgcca
     4861 tggcgttgaa cgagagctcg agaaagccgg cggcgaagag cagcgccagc gcgagcgcat
     4921 acgaggtcga cacg
//
LOCUS       DASYJA010000011         3066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3180097, whole genome shotgun sequence.
ACCESSION   DASYJA010000011 DASYJA010000000
VERSION     DASYJA010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3180097"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VGO02_00245"
     CDS             <1..409
                     /locus_tag="VGO02_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEV7476145.1"
                     /translation="NRALENIQGETAVHICFGYAAIIHARPEGYSFLPEFAGCTCKQV
                     SIETAQSNLDCSVLTSLKDKKIMVGVIDLSDMTIETPQTVVERAQRALKHLKPENMIL
                     APDCGMKYLPREVADGKMKALVEGAKLLRAQYR"
     gene            449..1126
                     /locus_tag="VGO02_00250"
     CDS             449..1126
                     /locus_tag="VGO02_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEV7476146.1"
                     /translation="MKSYIALTVAAAVLSGCAAGGPGFGQDNRPFTNTESGILIGAAS
                     GAVLGAVAYKSNRSRGAVIGAIGGGIAGGAVGSYMDKQKQDLEKSLAQEIQAGHARID
                     KMPNDVVRITMTNQTAFETDSSTIKQGFNPTMDKVADVVLRYGKTTLTVVGHTDNVGS
                     NDYNQKLSERRAHSVAEYLESKRVNGMRLALAGKGETQPIASNASETGRQANRRVEIY
                     VEPVVEG"
     gene            1129..2829
                     /locus_tag="VGO02_00255"
     CDS             1129..2829
                     /locus_tag="VGO02_00255"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEV7476147.1"
                     /translation="MPEKKPQGLARGLTNYGDAGFSLYLRRSFAKSMGYSSDMLARPV
                     VGIADSRSGFNNCHRHFPELIEAVKRGVLAAGGLPIEFPTVSLGEVFLSPTSMMFRNL
                     MAIDVEEMIRAQPMDAVVLVGGCDKTVPAQLMGAASADVPAVQLLAGPMMPTSFRGER
                     LGACTDCRRFWALHRAGKVDSSEIETIEGNLATTAGTCAVMGTASTMASIAEGLGMAL
                     PGTAAIPAVHADRLRAAEASGRRAVEMVSNPIRPSQIITEKSVENALRVLLAIGGSTN
                     AIVHLTAIAGRVGVQVSLNNLNKLSDSTPVLVDLKPTGQHYMSDLYAAGGVGAVLREL
                     KPLLNLDCMTVAGVTLGEVLDKQNSWVDRAVVREFKDPISRVGGLVALFGSLAPNGAI
                     LKRSAADPSLFEKEGRAVVFSSLDDLAARVDSPELDIKPDDFMVLQNAGPKSGYAMPE
                     AGYLPIPQKLARAGLKDMVRISDARMSGTAYGTIVLHVSPEAAVGGALALVRNGDRIK
                     ISVKQRRIDLLVGDAELQKRKAAWKPPVTAPTRGYAKLYMDHVLQAEHGCDFDFLRKT
                     "
     gene            complement(2830..>3066)
                     /locus_tag="VGO02_00260"
     CDS             complement(2830..>3066)
                     /locus_tag="VGO02_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476148.1"
                     /translation="ALRKPLVDDVGVLYMVTLPDGRIEPFTPCDPRLPPVKLVGLRDN
                     QPEKDRGWIKMVLTMQPDASFNIKYEYPEPKPKK"
BASE COUNT          567 a          977 c         1009 g          513 t
ORIGIN      
        1 gaatagagca ttggagaata tccaaggaga aaccgcggtg cacatctgct tcggctacgc
       61 ggcgatcatc catgcgcggc ccgagggcta ttcgttcctg ccggagtttg caggctgcac
      121 ttgcaagcaa gtgtcgatcg agacggccca gtcgaacctg gattgttctg ttttgacttc
      181 actaaaagac aagaaaatca tggtcggcgt gatcgacctg tccgacatga cgatcgagac
      241 gccgcaaacg gtggttgagc gcgcgcagcg tgcgctcaag catctgaagc ctgagaatat
      301 gatcctggcg cccgattgcg gcatgaagta cctgccgcgc gaggtcgccg acggcaagat
      361 gaaagcgctc gtcgaaggcg cgaagctgtt gcgggcgcag taccgctgac gggaatccgc
      421 taggggcaat ttgacacaag gagtgagcat gaagtcgtac atcgcattga ccgtcgcagc
      481 cgctgtcctg tccgggtgcg ccgccggcgg ccccggcttc ggccaggaca accggccgtt
      541 cacgaatacc gaaagcggca tcctgatcgg cgccgcgagc ggcgccgtgc tgggcgcggt
      601 cgcctacaaa tcgaatcgca gccgcggcgc ggtgataggc gccatcggcg gcggcatcgc
      661 cggcggcgcg gttgggtcct acatggacaa gcagaagcag gacctcgaga agagcctggc
      721 gcaggagatc caggccggcc acgcgcgcat cgacaagatg ccgaacgacg tggtgcgtat
      781 caccatgacc aaccagaccg cgttcgagac cgactcgagc accatcaagc agggcttcaa
      841 ccccaccatg gacaaggtcg cggacgtggt cctgcgctat ggcaagacca ctctcacggt
      901 cgtcgggcat acggacaatg tcggcagcaa cgactacaac cagaagctgt ccgagcggcg
      961 cgcgcattcc gtggccgagt acctcgagtc caagcgcgtc aacggcatgc gcctcgcgct
     1021 cgccggcaag ggcgagaccc agccgatcgc gagcaacgcc tcggagacgg gccgccaggc
     1081 gaaccgccgc gtcgagatct atgtcgagcc ggtagtggaa ggatagagat gccggagaag
     1141 aagccgcagg ggctggcgag gggcctcacc aactacggtg acgccgggtt ctctctttac
     1201 ctgcgccgtt ccttcgccaa gagcatgggc tattccagcg acatgctggc gaggcccgtg
     1261 gtcggcatcg ccgactcgcg cagcggcttc aacaactgcc accgccactt ccccgagctg
     1321 atcgaggcag tgaagcgcgg cgtgctcgcc gcgggcggcc tgccgatcga gttccccacg
     1381 gtctcgctcg gcgaggtgtt cctcagcccg acctcgatga tgttccgcaa cctgatggcc
     1441 atcgacgtcg aggagatgat ccgcgcccag ccgatggacg cggtggtgct ggtcggcggc
     1501 tgcgacaaga cggtgccggc gcagctcatg ggcgcggctt cggccgatgt tccggcggtg
     1561 cagctgcttg ccggtccgat gatgccgacc tcgttccgcg gcgagcgcct gggcgcctgc
     1621 accgactgcc gcaggttctg ggcgcttcat cgggcaggca aagtggattc ttctgaaatt
     1681 gaaaccatag aaggcaacct ggccaccacc gccggcacgt gcgccgtgat gggcaccgcc
     1741 tcgaccatgg cttccattgc ggagggcctc ggcatggcgt tgccaggcac ggcggcgatt
     1801 cccgcagtgc acgcggaccg gctgcgtgca gccgaagcga gcggcaggcg cgccgtggag
     1861 atggtgagca acccgatccg cccctcgcag atcatcacgg agaaatccgt ggagaacgcg
     1921 ctgcgcgtgc tgctggcgat cggcggctcg accaacgcca tcgtgcatct cacggcgatc
     1981 gcgggacgcg ttggcgtgca ggtttccttg aacaatctga ataaattatc cgattcaacg
     2041 ccggtgctgg tggacctcaa gccgaccggg cagcactaca tgtcggacct ctacgccgcc
     2101 ggcggcgttg gcgcggtgct gcgcgagctg aagccgctgc tgaatctgga ttgcatgacc
     2161 gtcgccggcg tcaccttggg cgaggttctc gacaagcaga attcatgggt ggaccgcgcc
     2221 gtggtgcgcg aattcaagga cccgatctcg cgggtgggcg ggctggtggc tttgttcggc
     2281 tcgctcgcgc ccaacggcgc gatcctcaag cgctcggccg ccgacccgtc gctgttcgag
     2341 aaggagggcc gcgcggtggt cttctcctcg ctcgacgacc tggcggcgcg cgtcgattcg
     2401 cccgagctcg acatcaagcc cgacgacttc atggtgctgc agaacgccgg gccgaagtcg
     2461 ggctacgcca tgcccgaggc cggctacctg ccgatcccgc agaagctggc gcgcgccggg
     2521 cttaaggaca tggtgcgcat ctcggacgct cgcatgagcg gcaccgcgta cggcaccatc
     2581 gtgctgcacg tctcgcccga ggccgcggtc ggtggggcgc tagctctcgt caggaacggg
     2641 gacaggataa aaatatccgt gaaacaacgg cgcattgacc ttctcgttgg tgatgctgag
     2701 ctgcagaaac gaaaagccgc ctggaagccg ccggtcacgg cacccacgcg cggctatgcc
     2761 aagctctaca tggaccacgt gctgcaggcc gagcacggct gcgatttcga ctttctgcgg
     2821 aaaacctagc tacttcttcg gcttcggctc cgggtattcg tacttgatgt tgaagctcgc
     2881 gtcgggctgc atggtcagga ccattttgat ccagccgcgg tccttctccg gctggttgtc
     2941 gcgcaagccg accagcttca ccggcggcag ccgcggatcg cacggcgtga atggctcgat
     3001 ccgcccgtcc ggcagggtga ccatatagag cactcccacg tcgtcgacca acggcttcct
     3061 caacgc
//
LOCUS       DASYJA010000012         5010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1273431, whole genome shotgun sequence.
ACCESSION   DASYJA010000012 DASYJA010000000
VERSION     DASYJA010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273431"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VGO02_00265"
     CDS             <1..471
                     /locus_tag="VGO02_00265"
                     /inference="COORDINATES: protein motif:HMM:NF015909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476149.1"
                     /translation="RSHYEDVLVVRVRGLAPKSLWQERYGHIADFEELLAEHGTIVLK
                     FFLHITKAEQKKRLLERERSPETAWKLNPNDWIDRDYWDDYTEAYEDAISKTAAPHAP
                     WIVVPANAKWYRNLLVAETVVEALRGQRNGWKKTLDKMGRQRRAELAAYRAQRR"
     gene            468..1418
                     /locus_tag="VGO02_00270"
     CDS             468..1418
                     /locus_tag="VGO02_00270"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7476150.1"
                     /translation="MRKILFATLLAATAASAQEWPSKPIRWVVPYPPGGSVDIVSRLM
                     QTGVSEGLGQPIVIESRAGAGGAVGSAEVARSAPDGYTFLFTLSSHTINPHLYKLSYD
                     VERDFASVSLIVSIPQMLAVNAATPYKSINDVVAAARAQPGKLSFASAGNGTPSHIAG
                     ELLKLRTGIDLVHVPYKGGGPAMTDTLAGQVPMLFLTAAIVMPHARSGRLKPLAVTTL
                     KRSPGAPEVPTVAEALGLPDYEVDSWIAMFAPAKTPPAVIARMQKEIARVARLPQMRQ
                     KLIEQTADPVASTPEELDRVVKAEMKKWAQVIRSAGIRAD"
     gene            complement(1415..3979)
                     /locus_tag="VGO02_00275"
     CDS             complement(1415..3979)
                     /locus_tag="VGO02_00275"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEV7476151.1"
                     /translation="MSAVLRPSDALREQVAAGRAALRQAYLQKPEPRQLLQRHAHLID
                     RTVKAVWAQIEPAGSAALVATGGYGRGELYPCSDIDLLVLLPGEPGPGERERVERLIG
                     TFWDIGLEIGHSVRTVQGCLDTAAADITIQTTLLEARFLTGSRRLFRGLQAGLEEQLD
                     PVAFFKAKLLEQEQRHAKHQDSPYSLEPNLKEAPGGLRDLQTIQWIARASGMGRSWNH
                     LVAHGLLERHEARQLARTEAAFQDMRIRLHYLAGRREDRVVFDFQGALAAQFGYADTP
                     DLRASEAMMQRYYRAAKTVTQLNAILLQNLQARLMPLHDVPLRPLNERFQVRGELLEA
                     VEEDLFERQPTAILESFLLMMQHPELRGMSAPTLRALWRARRRIDARFRRDPTARLLF
                     LHILQQPRGIVHEFRRMNQYGILGRYLPEFGSIVGQMQHDLFHVYTVDQHILMVLRNV
                     RRFAMPEFAHEFPLCSELISGFERHWLLYVAALYHDIAKGRGGDHSALGATDVRNFCK
                     RHGLSAEDTDLIAFLVEHHLAMSAVAQKQDVHDPEVVRAFAQRVATERRLVALYLLTV
                     ADVRGTSPKVWNAWKAKLLEDLFRAARRALTGQPQARDAALAEKQAEAARLLKLYDLS
                     DSVKERLWAQLDTAYFLRHDPQEIAWQTRTLHYRVDADQPVAKARLAPFGEGLQVMIY
                     TKDRESLFARICGYFERAGFNIVEAKVHTTRHGYALDTFLVLGQGADAHYRDMIERIE
                     AELPREVDSQAPLDPPRGGRLSRRVRHFPISPVVNIRPDERGSYQVLTLVAADRPGLL
                     YGVARMLARYQINLQTARINTLGDRAEDVFLVTGEALSNSKKVLQLEQELLKELQI"
     gene            complement(3976..4785)
                     /gene="map"
                     /locus_tag="VGO02_00280"
     CDS             complement(3976..4785)
                     /gene="map"
                     /locus_tag="VGO02_00280"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEV7476152.1"
                     /translation="MAVVLKTPEEIGRMRIAGKLASEVLDFITPHVRAGVTTGKLNDL
                     CHDYMVDVQKTVPAPLNYAPPGYKPYPKSICTSVNHQVCHGVPGDRVLRAGDILNIDI
                     TVIKDGYHGDCSRMFYVGEPSIQARRLVDVTHECMWLGIREVRPGARLGDLGAAIQSH
                     AEKHGFSVVREFCGHGIGRKFHEEPQVLHYGKAATGLVLEPGMTFTVEPMINAGKPGI
                     RELADGWTIVTKDHSLSAQWEHTVAVTESGVEVLTTSAGAVPAPALLSASR"
     gene            4926..>5010
                     /locus_tag="VGO02_00285"
     CDS             4926..>5010
                     /locus_tag="VGO02_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEV7476153.1"
                     /translation="MSVTMREMLEAGVHFGHQTRYWNPKMAP"
BASE COUNT          787 a         1661 c         1765 g          797 t
ORIGIN      
        1 cgctcgcact acgaagacgt tctggtggtc cgggtgcgcg gcctggcgcc gaaaagcctg
       61 tggcaggagc gctacggcca catcgcggac ttcgaggagc tgctcgccga gcacggcacc
      121 atcgtgctga agttcttcct gcacattacg aaggcagagc agaagaagcg gctgctcgag
      181 cgcgagaggt cccccgagac cgcctggaag ctcaatccga acgactggat cgaccgcgat
      241 tactgggacg attacaccga agcctacgag gacgcgatct ccaagaccgc ggcgccgcac
      301 gcgccgtgga tcgtggtgcc ggccaacgcc aagtggtatc gcaacctcct cgtcgccgaa
      361 accgtcgtcg aggcgctacg cgggcagcgc aacggctgga agaagactct cgacaagatg
      421 ggccggcagc gccgcgccga gctcgccgcc taccgggcgc agcgccggtg agaaagatcc
      481 ttttcgcaac gctgctcgcg gccacggcag ctagcgcgca ggagtggccg agcaagccaa
      541 tccgctgggt cgtaccgtat ccgccgggcg gcagcgtcga catcgtctcg cgcttgatgc
      601 agaccggcgt gtcggaaggc ctcggccagc cgatcgtcat cgagagtcgg gccggcgccg
      661 gcggcgccgt gggcagcgct gaagtcgcac gctcggcgcc cgacggctat acctttctct
      721 tcacgctctc ctcgcacacg atcaatccgc acctctacaa gctcagctac gacgtcgagc
      781 gcgatttcgc gtcggtgtcg ctcatcgtga gcatcccgca gatgctcgcc gtgaacgcgg
      841 ccaccccgta caagtcgatc aacgacgtgg tcgcggccgc ccgggcgcaa ccaggcaagc
      901 tttcgttcgc ttcggcaggc aacggcacgc cttcccacat cgccggcgag ctgctcaagc
      961 tcaggaccgg catcgatctc gtgcacgttc cgtacaaggg cggggggccg gcgatgaccg
     1021 acacgctggc cggccaggtg ccgatgctct tcctgaccgc ggcgatcgtg atgccgcacg
     1081 cgcgctccgg gaggctgaag ccgctcgcgg tgacgaccct caagcgctcg ccgggcgcgc
     1141 cggaggtccc cacggtggcc gaggcgctcg ggctgcccga ctacgaagtc gattcgtgga
     1201 tcgcgatgtt cgcgccggcg aagacgccgc cggccgtaat cgcaaggatg cagaaggaga
     1261 tcgcgcgcgt cgcccggctt ccgcagatgc gccaaaagct gatcgagcag accgccgatc
     1321 cggtggccag cacgcccgaa gagctcgacc gcgtggtaaa ggcggaaatg aagaaatggg
     1381 cgcaagtgat ccgctcggcc gggatccggg ccgactagat ctgcagctct ttcagcagct
     1441 cctgctcgag ctgcagcacc ttcttcgagt tggacagcgc ttcgcccgtc accaggaaca
     1501 cgtcctcggc gcggtcgccg agcgtgttga tgcgcgcggt ctgcaggttg atctggtagc
     1561 gcgccagcat gcgggcgact ccgtacagca ggcccggccg gtcggccgcc accagcgtca
     1621 gcacctgata ggagccgcgc tcgtccgggc ggatgttcac caccggggag atcgggaagt
     1681 ggcgcacgcg gcgcgagagg cgcccgccgc gcggcgggtc gagcggcgcc tgcgaatcga
     1741 cctcgcgcgg cagctccgcc tcgatgcgct cgatcatgtc gcggtagtgc gcgtcagcgc
     1801 cctggccgag gacgaggaag gtgtcgagcg catagccgtg gcgcgtggtg tgcaccttgg
     1861 cctcgacgat gttgaagccg gcgcgctcga aatagccgca gatgcgcgcg aacagcgact
     1921 cgcggtcctt cgtgtagatc atcacctgca ggccctcgcc gaagggcgcg aggcgcgcct
     1981 tcgccaccgg ctggtcggcg tcgacgcggt agtgcagggt gcgcgtctgc caggcgatct
     2041 cctgcgggtc gtggcgcagg aagtaggcgg tgtcgagctg cgcccacagc cgctccttga
     2101 cgctgtccga gaggtcgtag agcttcagca ggcgcgcggc ttcggcctgc ttctcggcga
     2161 gcgcggcgtc gcgcgcctgc ggctgtccgg tgagcgcgcg gcgcgcggcg cggaataggt
     2221 cctcgagcag cttggctttc cacgcgttcc agaccttggg gctggtgccg cgcacgtcgg
     2281 cgacggtgag caggtagagc gcgaccagcc tgcgctcggt cgccacgcgc tgggcgaacg
     2341 cgcgcaccac ctcggggtcg tgcacgtcct gcttctgcgc gaccgccgac atcgccaggt
     2401 ggtgctcgac gagaaacgcg atcagatcgg tgtcttcggc cgacaggccg tgccgcttgc
     2461 agaaattcct gacatcggtg gcgcccagag ccgaatggtc gccgccgcgt cctttggcga
     2521 tatcgtggta gagcgccgcg acatacagca gccagtgccg ctcgaagccg ctgatcagct
     2581 cgctgcacag cgggaactcg tgcgcgaact cgggcatggc gaagcggcgc acgttgcgca
     2641 gcaccatcag gatgtgctgg tcgacggtgt agacgtggaa caggtcgtgc tgcatctgcc
     2701 cgacgatgga gccgaactcg ggcaggtagc ggccgaggat gccgtactgg ttcatgcggc
     2761 ggaactcgtg cacgatgccg cgcggctgct gcaggatgtg caggaacagc aggcgcgcgg
     2821 tcgggtcgcg gcggaagcgc gcgtcgatgc gccgccgcgc gcgccacagc gcgcgcagcg
     2881 tcggcgcgct catgccgcgc agctccgggt gctgcatcat cagcaggaag ctctcgagga
     2941 tcgccgtcgg ttgccgctcg aacagatctt cctcgactgc ctccagcaat tccccgcgca
     3001 cctgaaagcg ctcgttgagc ggccgcagcg gcacgtcgtg cagcggcatc agccgcgcct
     3061 gcaggttctg cagcaggatc gcgttgagct gcgtcacggt cttcgcggcg cggtagtagc
     3121 gctgcatcat cgcttcgctc gcgcgcaggt ccggcgtgtc ggcgtagccg aactgcgcgg
     3181 caagcgctcc ctggaagtcg aacacgaccc ggtcctcgcg ccgcccggcg aggtagtgca
     3241 ggcggatgcg catgtcctgg aaggccgcct cggtgcgcgc cagctggcgc gcctcgtggc
     3301 gctctaggag gccgtgcgcc accaggtggt tccagctgcg gcccatgccg cttgcgcggg
     3361 cgatccactg gatggtctgc aggtcgcgca ggccgccggg cgcctccttg aggttgggct
     3421 cgagcgaata gggcgagtcc tggtgcttgg cgtggcgctg ctcctgctcg agcagcttcg
     3481 ccttgaagaa ggcgaccggg tcgagctgct cctcgaggcc ggcctgcagc ccgcggaaca
     3541 ggcgccggct gccggtgagg aagcgcgcct ccagcaaggt cgtctggatg gtgatgtcgg
     3601 cggcggcggt gtcgaggcag ccctgcacgg tgcgcacgct gtggccgatc tccaggccga
     3661 tgtcccagaa ggtgccgatc aggcgctcga cgcgctcgcg ctcgcccggt ccgggctcgc
     3721 cgggcagcag caccagcagg tcgatgtccg agcacgggta gagctcgccg cgcccgtatc
     3781 cgccggtcgc gaccagggcg gcggagccgg cgggctcgat ctgcgcccac accgccttga
     3841 ccgtgcggtc gatgaggtgc gcgtggcgct ggagcagctg ccgcggctcg ggcttctgca
     3901 ggtacgcctg gcgcagcgcg gcgcgtccgg ccgcgacctg ctcgcgcagg gcgtccgacg
     3961 gccgcagcac cgcgctcatc ggctggcgga gagcagggcc ggggccggca ccgcgccggc
     4021 ggaggtggtc agcacttcga cgccgctctc ggtgaccgcg acggtatgct cccactgcgc
     4081 cgagaggctg tgatccttgg tgacgatggt ccagccgtcg gcgagctcgc ggatgccggg
     4141 cttgccggcg ttgatcatcg gctcgacggt gaaggtcatg ccgggctcga gcaccaggcc
     4201 ggttgccgcc ttaccgtagt gcaggacctg gggctcctcg tggaacttgc ggccgatgcc
     4261 gtggccgcag aactcccgca ccaccgaaaa gccgtgcttc tcggcgtggc tctggatggc
     4321 ggcgccgagg tcgcccaggc gcgcccccgg gcgcacctcg cggatgccca gccacatgca
     4381 ttcgtgcgtc acgtcgacca gccggcgcgc ctggatgctc ggctcgccga cgtagaacat
     4441 gcggctgcag tcgccgtggt agccgtcctt gatgacggtg atgtcgatgt tgaggatgtc
     4501 gcccgccctc agcacccggt ccccgggcac gccatggcac acctgatgat tcaccgaggt
     4561 gcagatcgac ttggggtagg gcttgtagcc cggcggggcg tagttgagcg gcgccggcac
     4621 ggtcttctgc acgtcgacca tgtagtcatg gcacaggtcg ttcagcttcc cggtggtgac
     4681 ccctgcccgc acgtgggggg taatgaagtc gagcacctcc gaggcgagct tgcccgcaat
     4741 gcgcatgcgc ccgatctctt ccggggtctt caggactaca gccattggtc tgattatgct
     4801 agaattttgg gctgacttgc ggctttgcaa gtcaaatcta accgctccga tcgaataggg
     4861 tgccccaaaa ggaaaactgc ggggttcttg atacgggtgc gctgataacc ctaaaggaga
     4921 agcaagtgtc ggtcacgatg cgtgaaatgc tggaagccgg ggtccatttc ggacatcaga
     4981 cccggtactg gaacccgaag atggcgccgt
//
LOCUS       DASYJA010000013         1923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_366039, whole genome shotgun sequence.
ACCESSION   DASYJA010000013 DASYJA010000000
VERSION     DASYJA010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366039"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VGO02_00290"
     CDS             complement(<1..300)
                     /locus_tag="VGO02_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HEV7476154.1"
                     /translation="MSSERAYREQIARLYARSPFYRDKLRAAGFADAAAAGGLDDIAR
                     LPFTEKDELRKSQAEHPPLGAHAAVSLEEAARIYSTSGTSGVPLYIPLSRRDLDDW"
     gene            351..1232
                     /locus_tag="VGO02_00295"
     CDS             351..1232
                     /locus_tag="VGO02_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV7476155.1"
                     /translation="MNSRIGSLPPAVRGTLWMGGAVLSFSAMAVAVRQLLTHMAVFQI
                     LFLRTFIALLVVLAVAAAYGWFARLRTRRIGIHFLRNFTHFGGQYCWVYAIGALPLAT
                     VFAIEFTMPVWVALLAWWFLGERLNGPRLVMLALGLTGVLVILKPGVGVIHPAALVML
                     LGALCYAANLITTKTLTGTDSVLAILFWMNLIQTPLALIPALPQWSAPTLADAPWIAA
                     LAGGSLFAHFCMTRAFKLADATVVVPIDFMRLPLIAVVGAFAYGEALDPWVFVGAAII
                     FTGTYYSLSREARRVPA"
     gene            complement(1242..>1923)
                     /locus_tag="VGO02_00300"
     CDS             complement(1242..>1923)
                     /locus_tag="VGO02_00300"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEV7476156.1"
                     /translation="SFSLLALTMALVAVDASAQTPPAIQGPVYVATYVEFLPTAIGGG
                     AALLKQYRDASRNDTGNQRMELVQELGRPTRFALVSIWADQTAYDGHGRAAHTARLRD
                     QLRAVHAAPYDERVHGGMAVGARNELRAGAVIVLTHVDVPGTFKDATVPLLHQLAEAS
                     RKEAGCLRFEVQQQGNRQNHFTVVEHWADAKAYDGHVMADHTRQFRDKLGPMVGALYD
                     ERLYKALD"
BASE COUNT          258 a          687 c          630 g          348 t
ORIGIN      
        1 ccagtcgtcg aggtcgcgcc ggctgagcgg gatgtagagc ggcacgccgc tggtgccgct
       61 ggtcgaatag atgcgcgcag cctcttccag cgataccgcg gcgtgcgcac cgagcggcgg
      121 atgctcggcc tggctcttcc tcagctcgtc cttctcggtg aagggcagtc ttgcaatgtc
      181 gtccagcccg cccgccgccg ccgcgtcggc gaagccggcg gcgcgcagct tgtcgcgata
      241 gaacggcgag cgcgcgtaca ggcgcgcgat ctgctcgcga taggctcgtt cgctggacat
      301 ggggcgcaaa tcatcccaca tccgtaccat gcgggaatgg tcccaactcc atgaattcac
      361 gtataggttc gctgccgccg gcggtgcgcg gcaccctgtg gatgggcggc gcggtcctgt
      421 ccttctccgc catggcggtc gcggtgcggc agctgctgac gcacatggcg gtgttccaga
      481 tcctgttcct gcgcacgttc atcgcgctgc tggtggtcct cgccgtggcc gcggcctacg
      541 gctggttcgc gcgcctgcgc acccggcgca tcgggattca tttcctgcgc aacttcacgc
      601 acttcggcgg ccagtactgc tgggtctacg ccatcggcgc gctgccgctc gccaccgtgt
      661 tcgccatcga gttcaccatg ccggtgtggg tggcgctgct cgcctggtgg tttctcggcg
      721 agcggctcaa cggcccgcgc ctggtcatgc tggccctggg cctgaccggc gtgctggtca
      781 tcctcaagcc aggcgtcggc gtgattcatc ccgccgcgct ggtcatgctg ctcggcgcgc
      841 tgtgctatgc cgcaaacctg atcacgacca agaccctgac cgggaccgac tcggtgctcg
      901 ccatcctctt ctggatgaac ctgatccaga cgccgctcgc gctcatcccg gcactgccgc
      961 aatggtccgc gccgacgctc gccgacgcgc cctggatcgc cgcgctggcc ggcggcagcc
     1021 tgttcgcgca cttctgcatg acgcgcgcct tcaagctggc cgacgccacg gtggtggtgc
     1081 cgatcgactt catgcgcctg ccgctgatcg ccgtggtcgg cgccttcgcc tacggcgagg
     1141 cgctcgaccc ctgggtcttc gtcggcgccg ccatcatctt caccggcacg tactacagcc
     1201 tctcccgcga agcgcgccgc gtccccgcct agccggcgct gctagtcgag cgccttgtac
     1261 agccgctcgt catagagcgc gccgaccatc ggcccgagct tgtcgcggaa ctggcgcgta
     1321 tggtcggcca tcacatgccc gtcataggct ttcgcgtcgg cccaatgctc gacgaccgtg
     1381 aagtgattct gccgattacc ctgctgctgc acctcgaagc gcaggcagcc ggcctccttg
     1441 cggctcgcct cggcgagctg gtggagcaag ggcacggtcg catccttgaa cgtgcccgga
     1501 acgtcgacat gggtcagcac gatgacggcg cccgcgcgca gctcgttcct cgcgcccacg
     1561 gccatgccgc catgcacgcg ctcgtcgtag ggcgccgcgt gtaccgccct gagctgatcg
     1621 cgcaagcgcg cggtgtgcgc cgccctgccg tggccgtcat acgcggtttg atccgcccag
     1681 atggagacga gcgcgaaacg ggtgggtcgg cccagctcct gcaccagctc catgcgctgg
     1741 ttgccggtgt cgttgcggct cgcgtcgcga tactgcttca gcagcgcagc gccaccgccg
     1801 atcgccgtcg gcaggaattc gacataggtc gcgacgtaga ccgggccctg gatggcgggc
     1861 ggcgtctggg cgctcgcatc gaccgccacg agggccatag tcaatgcaag aagcgagaaa
     1921 ctg
//
LOCUS       DASYJA010000014         2496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_729230, whole genome shotgun sequence.
ACCESSION   DASYJA010000014 DASYJA010000000
VERSION     DASYJA010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729230"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VGO02_00305"
     CDS             <1..608
                     /locus_tag="VGO02_00305"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV7476157.1"
                     /translation="VSESFLDEIAHATGKDPLALRLELTKDHPRANAVIRAVAEMADW
                     KKPRGKERALGIAFSDYHESFSAGIAEVSLDRKTGKIRVHHYWIAGDAGIIVQPENAH
                     AQLEGAVVYGLGSALSEELTYKDGAVQQTNFHQYQVTRMSDMPEIHTKVIVTDNAPTG
                     LGELGVPTVAPAIGNAVFKLTGKRLRQLPMSPQRVTATLKA"
     gene            complement(612..1421)
                     /locus_tag="VGO02_00310"
     CDS             complement(612..1421)
                     /locus_tag="VGO02_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEV7476158.1"
                     /translation="MKVRRIAGALGAEILDVDLSGSFSAEKIRQAFLEHQVIFFRDQN
                     LDPAQFMAFARAMGRPVEYPFVKGIAGFPEVIEVKKLEHERHNFGGLWHSDTAYLDEP
                     PMGSMLLAREVPPHGGDTLFASQYLAYESLSSAMQNLLAGLKAVNASSKANVTRTRED
                     RIKEQGGSGKEYEAEHPVVRTHPETGRKALYVNSGHTVRFAGMTEEESAPLLEFLFRH
                     QVKPEFTCRFSWRVGSLALWDNRCTQHNPINDYHGHRRVMHRITLAGDRPS"
     gene            complement(1425..2384)
                     /locus_tag="VGO02_00315"
     CDS             complement(1425..2384)
                     /locus_tag="VGO02_00315"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7476159.1"
                     /translation="MSRLIALLAAALVAGAAMAQSYPNRPVKVVVPWPPGQATDIAAR
                     VVAEKLQVALGQPFVIDNRPGAGGLIGSDVVAKSAADGYTLLAASAGPISIMPNLQKI
                     PYDPLKDFAPVSLIALVPFVLVSHPSFPAGDLKGFIANVRANPGKYSFSSSGTAASAY
                     MVTALFNSMAQLDAQHVPYKGSAPAITDIIGGQITYTIETVAATVGHVKAGRLKAYGV
                     SFAQRASAMPDVPTIAEAAPLPGYDIGAWIGYTAPAGTPREALNRLAAEMQKIAQAPD
                     IKERYLALGLDPISSTPDEMAIFMRQQQERYGSIIKSQGIKAE"
BASE COUNT          392 a          795 c          878 g          431 t
ORIGIN      
        1 tcgtctccga gagcttcctc gacgagatcg cgcatgccac cggcaaggat ccgctcgcgc
       61 tgcgcctcga gctgaccaag gatcatccgc gcgccaacgc ggtgatccgc gcagtcgccg
      121 agatggcgga ctggaagaag ccgcggggca aagagcgcgc gctcggcatc gctttctccg
      181 actaccacga gtcgttcagc gccggcatcg ccgaggtctc gctcgaccgc aagaccggca
      241 agatccgcgt gcatcactac tggatcgcgg gcgacgcggg catcatcgtg cagccggaga
      301 acgcccacgc gcagctcgag ggtgcggtgg tctatggact gggctcggcg ctgtccgagg
      361 agctgacgta caaggacggc gcggtgcagc agaccaactt ccaccagtac caggtcacgc
      421 gcatgagcga catgccggag atccacacga aggtgatcgt gaccgacaac gcgccgaccg
      481 gcctgggcga gctcggcgtg ccgaccgtgg cgccggcgat cggcaacgcg gtgttcaagc
      541 tgaccggcaa gcgcctgcgg cagctgccga tgtcgccgca gcgggtgaca gcgactctga
      601 aagcctagct cttagctggg gcggtctccg gcaagggtga tgcgatgcat tacccgccgg
      661 tggccgtggt agtcgttgat cgggttgtgc tgcgtgcagc ggttgtccca tagcgcgagc
      721 gagccgacgc gccacgagaa ccggcaggtg aactcgggct tcacctggtg gcggaacagg
      781 aactcgagca gcggtgcgct ttcttcctcg gtcatcccgg cgaagcgcac ggtgtggcca
      841 ctgttgacgt agagcgcctt gcggccggtt tccgggtgcg tgcgcaccac gggatgctcg
      901 gcctcgtatt ccttgccgct gccgccctgc tccttgattc gatcttcccg ggtgcgcgtg
      961 acgttcgcct tggatgatgc gttgaccgcc tttaagccgg cgagaagatt ctgcattgct
     1021 gaagagagcg attcataggc gaggtactgg ctggcgaaca gggtatcgcc gccatgtggc
     1081 ggcacctcgc gcgccagcag catcgagccc atcggcggct cgtcgaggta ggcggtgtcg
     1141 gaatgccaca ggccgccgaa gttgtggcgc tcgtgctcga gcttcttcac ctcgatcacc
     1201 tcgggaaagc cggcgatgcc cttgacgaac ggatactcga cgggccgtcc catggcgcgc
     1261 gcgaacgcca tgaactgcgc cgggtcgagg ttctggtcgc ggaagaaaat gacctggtgc
     1321 tcgaggaaag cttgcctgat tttttccgcc gaaaaacttc ctgagaggtc gacgtcgagg
     1381 atctcggcgc ccagcgcgcc ggcgatgcgc cgcaccttca ccgcctactc cgccttgatg
     1441 ccctggctct tgatgatcga gccgtagcgc tcctgctgct ggcgcatgaa gatcgccatc
     1501 tcgtcgggcg tgctggagat gggatcgaga ccgagcgcga gatagcgctc cttgatgtcc
     1561 ggcgcctgcg cgatcttctg catttccgcc gccagccggt tgagcgcttc gcgcggcgtg
     1621 cccgccggcg cggtgtagcc gatccaggcg ccgatgtcgt agcctggcag cggcgcggcc
     1681 tcggcgatcg tcggcacgtc cggcatcgcg ctggcgcgct gcgcgaagga gacgccgtag
     1741 gctttgaggc ggcccgcctt cacgtgcccg acggtcgcgg cgacggtctc gatcgtgtag
     1801 gtgatctggc cgccgatgat gtcggtgatg gcgggcgcgc tgcctttgta cggcacgtgc
     1861 tgcgcgtcga gctgcgccat cgagttgaac agcgcggtga ccatgtaggc cgacgcggcg
     1921 gtgcccgagg acgagaagct gtacttgccg gggttggcgc gcacgttggc gatgaatccc
     1981 ttcaggtcgc ccgccgggaa cgacgggtgg ctgacgagca cgaacggcac cagcgcgatc
     2041 aggctgaccg gcgcgaagtc cttgagcgga tcgtagggaa tcttctgcag gttcggcatg
     2101 atcgaaatcg ggccggcaga ggcggcgagc agggtatagc cgtccgccgc gctcttcgcc
     2161 accacgtcgc tgccgatcaa accgccggcg ccgggccggt tgtcgatgac gaacggctgg
     2221 ccgagcgcga cctgcagctt ctcggccacc acgcgcgccg cgatgtcggt ggcctgcccc
     2281 ggcggccagg gaacgacgac cttgaccggc cggttgggat agctttgcgc catggcggcg
     2341 cccgcgacga gcgctgcggc gaggagggcg atgaggcggc tcatggttgc tccagcttga
     2401 tgtttgcgtt gcggatgacg gcggcgtagc gctcctgctc cttgcgcatg aacgccggca
     2461 gctcttccgg cgtgctcgcc accgggtcga ggccgg
//
LOCUS       DASYJA010000015         1649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2635607, whole genome shotgun sequence.
ACCESSION   DASYJA010000015 DASYJA010000000
VERSION     DASYJA010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2635607"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1649)
                     /locus_tag="VGO02_00320"
     CDS             complement(<1..>1649)
                     /locus_tag="VGO02_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV7476160.1"
                     /translation="ANILIVDDLPEKLLVFKTVLEDLDQNLIMVRSGSEALREVLERE
                     FAVILLDVNMPDIDGIETASLIRGYKKSAHTPIIFITAYADEMQTAQGYSLGAVDYIL
                     SPVMPDVLRSKVKVFVELYQAQQRTKLMAEERVARAQAEAARHAAEENTRRADFMALA
                     SRELGASLDLEEGMRRLLDLAVPRFAAAATLLVDDESRGQWVCLASAEDLENFPYEEL
                     PRNLAASLQDVLKEGRVLRSGVPLRVGERRLGALLIEAGEADLATLEELANRAAMAFE
                     NARLYGSLQRESARSRAAEESQQEAARRKDEFLAMLSHELRNPLAPIRTAIEVIRRVA
                     PPDPTLTMARDAVDRQVGHLVRLVEELLDVSRISEGRITLKKEQIELGKVLAHSVETA
                     RPLIEARGQELSIRLPRSPVWVFGDAVRLAQVVANLLNNAAKYTPEGGHIQLCASAHE
                     GEAMVEVRDDGEGIDAQLLPRVFDLFVQGQRSLDRSQGGLGLGLTLVKRLIELHQGRV
                     EGESAGPGRGSAFRIFLPCLTAVVNRSEKIPSIQGRKTVMA"
BASE COUNT          238 a          601 c          540 g          270 t
ORIGIN      
        1 tgccatgacc gttttcctgc cttgaattga aggaatcttc tcggagcggt ttaccacggc
       61 ggtcaggcag ggcaggaaga tgcgaaatgc cgagccgcgc cctgggcccg cgctctcgcc
      121 ttcgacgcgc ccctggtgca gctcgatcag ccgcttcacc agcgtaagcc ccagccccag
      181 gccgccctgg ctgcggtcga gcgagcgctg accctgcacg aacagatcga acacgcgcgg
      241 caggagctgg gcgtcgatgc cctcgccgtc gtcgcgcacc tcgaccatcg cctcgccctc
      301 gtgcgcgctg gcgcagagct ggatgtggcc gccctccggg gtgtacttgg cggcattgtt
      361 gagcaggttg gcgactacct gcgcgaggcg caccgcgtcg ccgaacaccc acaccggcga
      421 gcgcggcagc ctgatcgaca gctcctggcc gcgcgcctcg atcagcggcc gcgccgtctc
      481 gacgctgtgc gcgagcacct tgccgagctc gatctgctcc ttcttcagcg tgatgcgccc
      541 ctcgctgatc cgcgacacat ccaatagctc ctccaccagg cgcaccaggt gacctacctg
      601 ccggtcgacg gcgtcccgcg ccatcgtcag ggtcggatcg ggcggggcga cgcgccggat
      661 cacctcgatc gcggtgcgga tcggcgccag cggattgcgc agctcgtgcg acagcatggc
      721 gaggaactcg tccttgcgcc gggctgcctc ctgctggctt tcctcggcgg cgcgcgagcg
      781 cgccgactcg cgctgcaggc tgccgtacag gcgcgcgttc tcgaacgcca tcgcggcgcg
      841 gttcgccagc tcctcgaggg tcgccaggtc ggcctcgccc gcctcgatca gcagcgcgcc
      901 caggcgccgc tcgccgacgc gcagcggcac cccgctgcgc agcacgcggc cttccttcag
      961 cacgtcctgc agggacgccg cgagattccg cggcagctcc tcatacggaa aattctcgag
     1021 atcttcggca gacgcaaggc agacccattg cccccggctt tcgtcgtcga cgagcagcgt
     1081 cgcagcggcg gcgaagcgcg gcaccgccag gtcgagcagc cgccgcatgc cctcctccag
     1141 gtcgagcgag gcgcccagct cgcggctggc gagcgccatg aagtcggcgc ggcgcgtgtt
     1201 ctcctcggcg gcgtggcgcg ccgcctcggc ctgggcgcgc gccacgcgct cctcggccat
     1261 cagcttcgtg cgctgctgcg cctggtagag ctcgacgaac accttgacct tgctgcgcag
     1321 cacgtccggc atcaccggcg acaggatgta gtcgaccgcg ccgagcgagt agccctgcgc
     1381 ggtctgcatc tcgtcggcgt aggcggtgat gaagatgatc ggcgtgtgcg ccgacttctt
     1441 gtagccgcgg atcagcgacg ccgtctcgat gccgtcgatg tcgggcatgt tgacgtcgag
     1501 caggatcacg gcgaactcgc gctccagcac ctcgcgcaac gcctccgacc ccgagcgaac
     1561 catgatcagg ttctggtcca ggtcctcgag gaccgtcttg aagaccagca gtttttctgg
     1621 caggtcgtcg acgatcagga tgtttgctc
//
LOCUS       DASYJA010000016         2967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_820844, whole genome shotgun sequence.
ACCESSION   DASYJA010000016 DASYJA010000000
VERSION     DASYJA010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820844"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..360
                     /locus_tag="VGO02_00325"
     CDS             118..360
                     /locus_tag="VGO02_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476161.1"
                     /translation="MPVHSRFRRSTGQCLAIGLLALPVAVHSAHAADEKPAEGNAVTR
                     TAKKAAKAVERGAKKADKALTNAVEKTDNWVKKKTK"
     gene            405..1532
                     /locus_tag="VGO02_00330"
     CDS             405..1532
                     /locus_tag="VGO02_00330"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7476162.1"
                     /translation="MFVGFAAAYSFAAFFSAFQSEFGASRGHVALVFSVAAFLWFLAG
                     APGGVLADRFGARRVALAGVACLVAALVLAAVSRSVEALYITYSVGIGLGVGLVYVPS
                     VGAVQPWFNANRAFASGIAVAGIGAGNIAGPLLAAWWIGLFGWRGAYVALAIFVLVLG
                     GVAASLLENISDAKNKNPVSVRDALGTRPFWLLYVSLTLSCIGLFVPMVHLSPYAQDL
                     GYSEAQGVTLVSLIGLGSLLGRFTVGPFADRMGREASLAAMYAGLGAMLLVWWAAAAW
                     WLLALFAIVFGICYGAYVALLPTIVMDLYGPRAVSGITGFLYTGAGIGTLFGPWLAGV
                     AYDALGAYDLPIIGGSLCAFLAAAIVVFMLRNEKAEVLHRG"
     gene            1501..2256
                     /locus_tag="VGO02_00335"
     CDS             1501..2256
                     /locus_tag="VGO02_00335"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEV7476163.1"
                     /translation="MKKQKSSIAVSDKYGYRTLHVGGEAIQSTMRISDPWSLALDYTR
                     CMMAFLLFHPAPRRALMIGLGGGSLAKFFHRRFRKTNVRVVELDERVVLAARTHFALP
                     PDDARLRVEVGDGAEALSPECCDVLVVDAYHDEEHVPTLASAEFYDAAYLALAEQGVM
                     VVNFMDDDRLLDEYLKRMEAAFGGHVLAMKALYDPNILAFALKGFGSTMPWETLRQRA
                     QALESRFDLPFPRYVSRFRAMNRVTPQALLLGP"
     gene            2253..>2967
                     /locus_tag="VGO02_00340"
     CDS             2253..>2967
                     /locus_tag="VGO02_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV7476164.1"
                     /translation="MIDPGQTAVLAAIAFLGALIFGITGFGAALVTIPLATHFVPLPF
                     ALALFAICDLAAALRIGLENPKNAVRSEWHRLIPMIFVGTALGVTLLVNLPKKTAMIA
                     LGVFILLFALYSLYPKKSQKLVASGWAWVAGLAGGITSTLFGAGGPPYVIYLSHRGLT
                     KEQFRATLGFTTMTSISLRVAAFLITGLLLNKDVWITALFGVPAILLGISVAQRIYMK
                     ISREALFKAVAVMLLASGSS"
BASE COUNT          413 a         1056 c          945 g          553 t
ORIGIN      
        1 aacaccacct cgttgagggc cttcatgagc agcgcgtgcg cagtattcat agcgccgtgc
       61 cgcttcacct tgagcaggat tctctgaatc ggcgagcgat ccggtatgct cggtttcatg
      121 ccggtccaca gtaggttccg ccggagcacg ggtcaatgcc tcgcaatagg cctgttggca
      181 cttcctgttg cggtgcactc agcgcatgcc gccgatgaga agccggccga aggcaacgcc
      241 gtcacccgca cagcgaagaa ggcggccaaa gcagtcgagc gcggcgccaa gaaagctgac
      301 aaggcgctga caaacgcggt ggaaaagacc gacaactggg tcaagaagaa aacgaagtag
      361 aggcgcccta cgggtgggtt gtcgtcgccg cggccttcac gctgatgttc gtgggcttcg
      421 cggcggccta ctcctttgcc gcattcttca gcgcgttcca gtcggagttc ggcgcctcgc
      481 gcgggcacgt ggcgctggtc ttctccgtgg cggcgttcct ctggttcctc gccggggcgc
      541 cgggcggcgt gctggccgac cgcttcggcg cgcgccgcgt ggcgctcgcc ggcgtcgcct
      601 gcctggtggc cgcgctagtg ctcgctgcgg tatcgcgctc ggtcgaggcg ctctatatca
      661 cctacagcgt cggtatcggc ctgggggtcg ggctggtcta cgttccctcg gtcggcgcgg
      721 tgcagccctg gttcaacgcg aaccgcgctt tcgcctcggg catcgcggtc gccggcatcg
      781 gcgccggcaa catcgccggg ccgctcctcg ccgcgtggtg gatcgggctc ttcggctggc
      841 gcggcgcgta cgtcgcgctg gcgatttttg tgctggtctt gggcggtgtc gccgccagcc
      901 tcctcgagaa catatcggat gccaagaaca agaacccggt atcggtgcgc gacgcccttg
      961 gtaccaggcc gttctggctg ctctacgtct cgctcacgct gagctgcatc gggctcttcg
     1021 tgccgatggt gcacctcagc ccctatgcgc aggacctcgg ctacagcgag gcgcagggcg
     1081 tgaccctggt cagcctgatc ggcctgggca gcctgctcgg gcgcttcacc gtcggcccgt
     1141 tcgccgaccg catggggcgc gaagcctcgc tcgccgccat gtacgcgggc ctgggcgcca
     1201 tgctgctcgt ctggtgggcg gccgccgcgt ggtggctgct cgcgctcttc gcgatcgtgt
     1261 tcggcatctg ctacggcgcg tacgtcgcgc tcctgcccac catcgtcatg gacctgtacg
     1321 gcccgcgcgc ggtctcgggc atcaccgggt tcctctacac cggcgccggc atcggcacgc
     1381 tgttcgggcc ctggctcgcc ggcgtcgcct acgacgcgct cggcgcctac gacctgccga
     1441 tcatcggcgg ttcgctctgc gcctttctcg ccgccgccat cgtggtattc atgctgcgca
     1501 atgaaaaagc agaagtcctc catcgcggtt agcgacaagt acggctaccg cacgctgcac
     1561 gtcggcggcg aggcgatcca gagcaccatg cggatctccg acccgtggtc cctcgcgctc
     1621 gactacacgc gctgcatgat ggccttcctg ctgttccatc cggcgccgcg gcgcgcgctg
     1681 atgatcggcc tgggcggcgg ctcgctggcg aagttcttcc accggcgctt ccggaagacc
     1741 aatgtgcgcg tggtcgagct cgacgagcgc gtggtgctcg ccgcccgcac gcacttcgcg
     1801 ctgccgccgg acgacgcgcg cctgcgcgtg gaggtgggcg acggagcgga agcgctttca
     1861 cccgagtgct gcgacgtgct ggtggtggac gcctaccatg acgaggagca cgtgccgacg
     1921 ctcgccagcg ccgagttcta cgacgcggcc tacctcgcgc tcgccgagca gggcgtgatg
     1981 gtggtcaatt tcatggacga cgatcgcctc ctcgacgaat acctgaagcg catggaggcc
     2041 gccttcggcg gccacgtgct cgccatgaag gcgctctacg acccgaacat cctcgccttc
     2101 gccctgaagg gtttcggttc aaccatgccg tgggaaactc tccgccagcg ggcgcaagcc
     2161 ctcgaatcgc gcttcgacct gccgttcccg cgctacgtct cgcgcttccg cgcgatgaac
     2221 cgggtcacgc cgcaggcgct gctcctcggg ccatgatcga ccccggccaa accgcggtcc
     2281 tggccgccat cgcctttctc ggcgcgctca tcttcggcat caccggcttc ggtgcggcgc
     2341 tggtcaccat cccgctcgcc acgcacttcg tgccgctgcc cttcgccctc gcgctcttcg
     2401 ccatctgcga cctcgccgcc gcgctgcgca tcggcctgga gaacccgaag aacgcggtgc
     2461 gcagcgagtg gcatcgcctc atcccgatga tcttcgtcgg caccgcgctc ggcgtcacgc
     2521 tgctcgtcaa cctgccgaag aagaccgcca tgatcgcgct gggcgtcttc atcctgctct
     2581 tcgctttgta cagtctttat ccgaagaaaa gccagaagct ggtggccagc ggatgggcct
     2641 gggtcgccgg gctggccggc ggcatcacca gcaccctctt cggcgccggc ggcccgccgt
     2701 acgtcatcta cctgtcgcac cgcggcctca ccaaggagca gttccgcgcc acgctcggct
     2761 tcaccacgat gaccagcatc tcgctgcgtg tcgcggcatt cctcatcacc ggcctgctgc
     2821 tcaacaagga cgtctggatc actgcgctct tcggcgtgcc cgccatcctg ctcggcatca
     2881 gcgtcgccca gcgcatctac atgaaaatat cgcgcgaggc cctcttcaaa gcagtcgcgg
     2941 taatgctcct cgcttcgggc agttccc
//
LOCUS       DASYJA010000017         3060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1093037, whole genome shotgun sequence.
ACCESSION   DASYJA010000017 DASYJA010000000
VERSION     DASYJA010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093037"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VGO02_00345"
     CDS             <1..900
                     /locus_tag="VGO02_00345"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7476165.1"
                     /translation="LHRAQGMKIGFIGLGAMGRHMAANLQRAGHELQGYDLRQVDGFS
                     NKKSAVEAMQGCELLFTSLPGPTEVEAVGQQVEGVSAWFDTSTNSPTVIRRIHDSLKK
                     KKIQFLDAPVSGGPKGAETGKLAIWVGGEQAVFDKYLSILKVIGDQPLYVGAIGAGTV
                     AKLAHNAASFAVQAALAEVFTLGVKGGVEPLALFKALRQGASGRKRTFDRLAEHFLPG
                     KYDPAAFSLRLAHKDVSLAMELARESGVPMKIGAIALQELEEGMRRGWSERDCRVTMT
                     LQSERAGVDVRVPPDKLGAALAD"
     gene            complement(897..1517)
                     /locus_tag="VGO02_00350"
     CDS             complement(897..1517)
                     /locus_tag="VGO02_00350"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEV7476166.1"
                     /translation="MSARARRALAIFGALLLCLAPASPRAQQKAERSAAELMDVLMWN
                     REPVGGPFALVDHTGTPRTDAQFHGKLLLLYFGFTSCPDVCPTDLQSIGLAMERLGKS
                     AQGVQPLFVTLDPERDTPQRLAGYVTFFHPRLIGLTGDADAIEQAARAYKVYYAKVPT
                     SGNGYTVDHSGYIYLMDRAGKYLGFFPPGTPPERMTDVIRPLLDAN"
     gene            complement(1514..2614)
                     /locus_tag="VGO02_00355"
     CDS             complement(1514..2614)
                     /locus_tag="VGO02_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7476167.1"
                     /translation="MNNLKRRDFLRLSAILTEAAAAGGLTRVAAAAPIAVPTVDKLTM
                     QVLVDGAFDLFSRPQKVNGVVVEVVRSADFARPFHAQWGLSLLLDSQRGGDARTILLD
                     FGHTPEALMLNMELQKIDPGKINALILSHGHNDHWGGLVGFIDKKRGLLPADLTLYAG
                     GEDNFCKRYAGASPGPFTDRGVLDRREMTARRVKLVLAQAPTVIENQAFTTGQIKRSG
                     IEKVLPNTMVEFAEKDGLGCNMSHFSPAELQGKIMPDEHVHEHATCFNLKDRGLVVIS
                     SCGHVGIVNSVRQAQEVSGVQKVHAIVGGFHLGPAPADYLSQIVAEVKKFQPDAVIPM
                     HCSGQNFIQAVREQMPDKLVLATIGSRIIFGT"
     gene            complement(2650..>3060)
                     /locus_tag="VGO02_00360"
     CDS             complement(2650..>3060)
                     /locus_tag="VGO02_00360"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEV7476168.1"
                     /translation="TVPAIQSVLAGDTAMAFGTIPGVAPQAKAGKLVLLGVSFPRRFS
                     QLPEVPPIGEQVAGFDMGFYTALFAPAKTPKEIIERLHAETMKALAHPKTREIYANSA
                     AEPGTMSSAELQAYVAREIREWAEVVRATGLKVD"
BASE COUNT          525 a          967 c         1036 g          532 t
ORIGIN      
        1 cttcaccgag cgcaaggtat gaagatcggc ttcatcggcc tgggcgcgat gggccgccat
       61 atggccgcca acctgcagcg cgccggccac gagcttcagg ggtacgacct tcggcaggtg
      121 gacggtttct ccaataaaaa atcggcggtc gaagcgatgc agggctgcga gctgctgttt
      181 acgtccctgc cgggcccgac cgaagtagag gccgtagggc aacaggtaga gggggtatcg
      241 gcgtggttcg acacgagcac taactcaccg acggtaatcc gtcgaattca tgattccctg
      301 aagaagaaga aaatccagtt cctcgatgcg ccggtcagcg gcgggccgaa aggcgcagag
      361 accgggaagc tcgcaatctg ggtgggtggc gagcaggcgg ttttcgacaa atatctttcc
      421 atcctcaagg tgatcggcga ccagccgctc tacgtcggcg ccatcggagc gggaacggtc
      481 gccaagctgg cgcacaacgc agcgagcttc gcggtgcagg cggcgctggc cgaggtgttt
      541 acattggggg tgaagggcgg cgtcgagccg ttggccttgt tcaaggcgtt gcgccagggc
      601 gcatccgggc gcaagcgcac cttcgaccgg ctcgcggagc acttcctgcc cggcaagtac
      661 gaccctgcgg cgttctcgct gcgcctcgcg cacaaggacg tgagcctggc gatggagctg
      721 gcgcgcgaat ccggcgtgcc gatgaagatc ggcgccatcg cgctgcagga gctggaggag
      781 ggcatgcgcc gcggctggag cgagcgcgat tgccgcgtca cgatgacgct gcaaagcgag
      841 cgcgccggcg tcgatgtgcg tgtgcccccg gacaagctgg gagcggcgct cgccgactag
      901 ttggcgtcga gcaacggccg gatgacgtcg gtcatgcgtt ccggcggcgt accgggcgga
      961 aagaagccga gatatttccc ggcccggtcc atcaggtaga tgtagccgga atggtcgacc
     1021 gtatagccgt tgccgctcgt cgggaccttg gcgtagtaga ccttgtacgc gcgagccgcc
     1081 tgctctatgg cgtccgcgtc gcctgtcaat ccgatcaacc gcgggtgaaa gaacgtcacg
     1141 tagccggcca ggcgctgcgg cgtgtcccgc tccgggtcga gcgtgacgaa aaggggctgc
     1201 accccttgcg cggatttccc gagccgctcc atcgccaggc cgatcgactg caggtcggtg
     1261 gggcagacat ccgggcagga cgtgaagccg aaatagagca ggagcagctt gccgtggaac
     1321 tgcgcgtcgg ttcgcggcgt cccggtgtga tcgaccagcg cgaacggtcc gccgaccggc
     1381 tcgcggttcc acatcagcac gtccatcagc tcggcggcgg agcgttcggc tttttgctgc
     1441 gcgcgcggcg atgccggcgc caggcacaac agcaatgcgc cgaaaatggc gagcgcgcgg
     1501 cgcgcccgcg cgctcacgtg ccgaagatga tccggctgcc gatcgtcgcc agcaccagct
     1561 tgtcgggcat ctgctcgcgc accgcctgga tgaagttctg gccgctgcag tgcattggaa
     1621 tgacggcatc gggctggaac ttcttgacct cggcgacgat ctggctgaga tagtcggccg
     1681 gagccggccc gagatgaaag ccgcccacga tggcgtgaac cttctgcacg ccggagactt
     1741 cctgcgcctg gcgcaccgag ttgacgatgc cgacatggcc gcaggagctg atcacgacca
     1801 gcccgcggtc cttcaggttg aagcaggtcg cgtgctcgtg cacgtgctcg tcgggcatga
     1861 tcttgccctg cagctcggcg gggctgaaat ggctcatgtt gcagcccagc ccgtccttct
     1921 ctgcgaactc caccatggtg ttcggcagca ccttctcgat cccgctgcgc ttgatctggc
     1981 cggtggtgaa agcctggttc tcgatcacgg tcggcgcctg ggccagcacc agcttcaccc
     2041 ggcgagccgt catctcgcgg cgatcgagca cgccgcggtc ggtgaacgga cccggcgacg
     2101 cgccggcgta gcgcttgcag aaattgtcct cgccgccggc atagagcgta aggtctgccg
     2161 gcagcaggcc gcgcttcttg tcgatgaagc cgaccaggcc gccccagtga tcgttgtggc
     2221 cgtggctgag aatgagcgca ttgatcttgc ctggatcgat cttctgcagc tccatgttaa
     2281 gcatcagggc ttccggcgtg tggccgaaat cgagcaggat cgtgcgggcg tcgccgccgc
     2341 gctgcgagtc gagcagcagc gaaaggcccc attgggcatg gaacggcctg gcgaaatcgg
     2401 ctgaacgcac gacttcaacc accacgccgt tcaccttttg cgggcgcgag aagagatcga
     2461 aagcgccgtc gaccagcacc tgcatggtga gcttgtcgac ggtcggcacc gcgatcggcg
     2521 cggcggccgc cacgcgtgtg aggcctccgg ccgcggcggc ttcggtgaga atcgcagaaa
     2581 gtctcaagaa gtcacggcgc ttgagattat tcatttttgt catcctccct tgttcggatt
     2641 ccgcgtcacc tagtctacct tcaagccggt ggcgcggacc acctcggccc actctctgat
     2701 ttcacgcgcc acgtaggcct gcagctcggc ggagctcatg gttccgggct ctgcggcgct
     2761 gtttgcatat atttcgcggg tcttcggatg ggcgagcgcc ttcatggtct cggcgtgcag
     2821 ccgctcgatg atttccttcg gcgtcttcgc aggcgcgaac agcgcggtgt agaagcccat
     2881 gtcgaaaccc gcgacctgct cgccgatcgg cggcacctcg ggcaactgcg aaaaacggcg
     2941 cgggaaggag acaccgagca gcaccagctt gccggccttc gcctgcggcg ccacgcccgg
     3001 gatggtgccg aaggccatcg cggtatcccc ggccagcacc gactggatcg ccggcaccgt
//
LOCUS       DASYJA010000018         2858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_457826, whole genome shotgun sequence.
ACCESSION   DASYJA010000018 DASYJA010000000
VERSION     DASYJA010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457826"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VGO02_00365"
     CDS             complement(<1..303)
                     /locus_tag="VGO02_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEV7476169.1"
                     /translation="MIKRFIRRVLGLPTESMVRMPRERHGLSRDSLSPAAAKVCAVLR
                     DAGFAGYVVGGAVRDLLLGIVPKDFDIATDARPEQVKPLFRRAIIIGRRFRLVHVMM"
     gene            complement(317..982)
                     /locus_tag="VGO02_00370"
     CDS             complement(317..982)
                     /locus_tag="VGO02_00370"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV7476170.1"
                     /translation="MRLALFDLDNTLLAGDSDYEWGQFLVDRGVLEREEYESQNRAYY
                     EQYAAGTLDIHEFLGFALRPLAAHQPADLERWHAEFMAERIRPMIATPARQLVRKHQR
                     AGDLCAIITATNSFVTRPIAREFGVEHLIATEPESKNGRFTGKVAGIPCFKEGKVKRL
                     DEWLVGRGKRLGDFAESTLYSDSHNDLPLLERVSRPVAVDPDEKLAARANERGWAVIS
                     LHG"
     gene            complement(1020..1430)
                     /locus_tag="VGO02_00375"
     CDS             complement(1020..1430)
                     /locus_tag="VGO02_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476171.1"
                     /translation="MRTKVALVALSLLAAAEAFAQAGLRVPDRAEPGLDPNFARGWLA
                     PEYDRFGFATYQWRDAAFGFSPTPRMSWAYSFGSRADVSLSYSNSYDYGQLSVFGRWW
                     FSSDWALSAETTSRDQPGFMPRVQDFRFGVQRRF"
     gene            complement(1536..2162)
                     /locus_tag="VGO02_00380"
     CDS             complement(1536..2162)
                     /locus_tag="VGO02_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HEV7476172.1"
                     /translation="MLESKFYMRQLPLEISPPAAPSLDNFVAGANAEALAAVRALAAG
                     SLSEVIVYLWGERGSGRSHLLRAATRANPGLIAADDVETLDPTAQQTLFNAINAARDG
                     GPAVLAAGGAPPGQLTLRDDVRSRLGWGLVYQLRAPGDAEKAGHLRAEAARRGLRLPE
                     EVLAYLLNHLPRDLASLNAVLDALDRYSLASQRPLTLPLVREALQKTH"
     gene            2161..>2858
                     /gene="purM"
                     /locus_tag="VGO02_00385"
     CDS             2161..>2858
                     /gene="purM"
                     /locus_tag="VGO02_00385"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007732089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEV7476173.1"
                     /translation="MAKGLSYRDAGVDIDAGDALVEAIKPFARRTMRPEVLAGIGGFG
                     ALCEIPKKYKHPVLVSGTDGVGTKLKLAFQLGRHDTVGIDLVAMSVNDVLVQGAEPLF
                     FLDYFACGKLEQGVAADVIKGIARGCELAGCALIGGETAEMPGMYPPGEYDLAGFCVG
                     VVEKARILDGKSIKPGDAILGLASSGPHSNGYSLIRKILEREPSKAAASLLEPTRIYV
                     KPILKLLESVPVKGL"
BASE COUNT          452 a          953 c         1032 g          421 t
ORIGIN      
        1 catcatcacg tgcaccaggc ggaagcgccg gccgatgatg atcgcgcggc ggaacagggg
       61 cttcacctgc tcggggcgcg cgtcggtggc gatgtcgaag tccttgggca cgatgcccag
      121 cagcaggtcg cgcaccgcgc cgcccaccac gtagccggcg aagccggcgt cgcgcagcac
      181 ggcacacacc ttggcggcgg cggggctgag ggaatcgcgc gacaggccgt gccgctcgcg
      241 gggcatgcgg accatggatt cggtgggcag gcccagcacg cggcggatga agcgtttgat
      301 cattcctgcc cggattctag ccgtgcaggg aaatcacggc ccaaccacgc tcgtttgccc
      361 tggcggcgag cttttcgtcg gggtccaccg cgaccggccg gctcacccgc tcgagaagcg
      421 gcaggtcgtt gtgcgaatcg ctgtacaggg tggactcggc gaagtcgccc aggcgcttgc
      481 cccgccccac gagccactcg tcaagacgct tcaccttgcc ttccttgaag cacgggatgc
      541 cagcgacctt gccggtgaag cggccgttct tcgactcggg ctcggtggcg atgaggtgct
      601 cgacgccgaa ctcgcgcgcg atcgggcggg tgacgaagct gttggtggcg gtgatgatgg
      661 cgcaaaggtc gccggcccgc tggtgcttgc gcaccaactg ccgcgccggc gtagcgatca
      721 tgggtcggat gcgctcggcc atgaactccg catgccagcg ctctaggtcg gccggctggt
      781 gcgcggcgag cggccgcagc gcgaagccga ggaactcgtg gatgtcgagc gtgccggcgg
      841 cgtactgctc gtagtaggcg cggttctgcg actcgtactc ctcgcgctcg agcacgccgc
      901 ggtcgacgag gaactggccc cactcgtaat cgctgtcgcc cgcgagcagc gtgttgtcga
      961 ggtcgaacag ggcgagccgc atggcccgcg agtttaactt cgaatcgaaa agcggctggc
     1021 tagaagcggc gctgcacgcc gaagcggaag tcctgcacgc gcggcatgaa gcccggctgg
     1081 tcgcgcgagg tggtctcggc gctcagcgcc cagtccgagg agaaccacca gcggccgaag
     1141 accgagagct ggccgtagtc gtagctgttg ctgtaggaca ggctgacgtc ggcgcgcgag
     1201 ccgaaggaat aggcccagct catgcgcggc gtgggcgaga agccgaacgc ggcgtcgcgc
     1261 cactggtagg tggcgaagcc gaagcggtcg tattcgggag ccagccagcc gcgcgcgaag
     1321 ttcgggtcga ggcccggctc ggcgcgatcc ggcacgcgca ggccggcctg cgcgaaggcc
     1381 tctgctgcgg cgagcagtga aagcgctacc agggcgacct tcgtacgcat aggggtatga
     1441 ccaacatact cccggcatcc ggccgttgtc gaggcagttt ttgccgttct tgcagaccct
     1501 gaccggcttt tgtggcgcaa ccgccacgcc ggctcttaat gcgttttctg aagagcttcc
     1561 cgcaccaggg gcagagtgag ggggcgctgg ctggcgagcg agtagcggtc gagggcgtcg
     1621 agcaccgcgt tcaggctggc gaggtcccgc ggcaggtggt tgaggagata ggcgaggacc
     1681 tcctcgggca ggcgcaggcc gcggcgcgct gcctcggcgc gcaggtggcc ggccttctcg
     1741 gcatcgcccg gcgcccgcag ctggtagacc aggccccagc cgagccggct gcgcacgtca
     1801 tcgcgcagag tgagctggcc gggcggcgcg ccgcccgcgg cgagcaccgc cgggccgccg
     1861 tcgcgcgccg cgttgatggc gttgaacagc gtctgctggg cggtggggtc gagcgtctcg
     1921 acgtcgtcgg cggcgatcag gcccgggttg gcgcgcgtcg ccgcgcgcag caggtgcgag
     1981 cggccgctgc cgcgctcgcc ccagagatag acgatcacct cggacagaga gccggcggcg
     2041 agggcgcgca ccgcggcgag cgcttcggcg ttggcgccgg cgacgaagtt gtcgagcgag
     2101 ggcgcggccg gaggcgaaat ctcgagggga agctgccgca tgtagaattt cgactctagc
     2161 atggcaaaag ggctctccta ccgcgacgcg ggcgtggaca tcgacgcggg cgacgccctc
     2221 gtcgaggcga tcaagccctt tgccaggcgc accatgcgcc ccgaggtgct cgccggcatc
     2281 ggcggcttcg gtgccctgtg cgagatcccg aagaagtaca agcacccggt gctcgtctcg
     2341 ggcaccgacg gcgtcggcac caagctcaag ctcgccttcc agctcggaag acacgacacc
     2401 gtcggcatcg acctcgtcgc gatgagcgtg aacgacgtgc tcgtgcaggg cgccgagccg
     2461 cttttcttcc tcgactactt cgcctgcggc aagctcgagc agggcgtcgc ggccgacgtg
     2521 atcaagggca tcgcgcgcgg ctgcgagctc gccggctgcg ccctgatcgg cggcgagacc
     2581 gccgagatgc ccggcatgta cccgcccggc gagtacgacc tggccggctt ctgcgtcggt
     2641 gtggtcgaga aggcgcgcat cctcgacggc aagtcgatca agcccggcga cgccatcctc
     2701 ggcctcgcct ccagcggccc gcactccaac ggctactcgc tcatccgcaa gatcctggag
     2761 cgcgaaccca gcaaggccgc cgcaagcctc ctcgagccga cgcgcatcta cgtcaagccg
     2821 atcttgaagc tcctggaaag cgtccccgtc aaaggcct
//
LOCUS       DASYJA010000019         3316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1274857, whole genome shotgun sequence.
ACCESSION   DASYJA010000019 DASYJA010000000
VERSION     DASYJA010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274857"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VGO02_00390"
     CDS             complement(<1..567)
                     /locus_tag="VGO02_00390"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEV7476174.1"
                     /translation="MKIACVGAGRMGRGIALAFTLAGRDITLVDLKARSDFDQLKTEA
                     MEEMEASLSMLVELGAIPRVDLSHVSVVQDPAALRDAELVFEAVPETLEAKRDAFARI
                     CGIISKNAILASTTSTILVTDLVPLVTHPERLLNAHWLNPAYLIPLVEISVHSRTSPE
                     IIKKLKALLEEAGKVPVVCGPTPGFIVPR"
     gene            complement(564..1592)
                     /locus_tag="VGO02_00395"
     CDS             complement(564..1592)
                     /locus_tag="VGO02_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HEV7476175.1"
                     /translation="MKIAVLGGGHGAYAAAADLAEQGHEIRHWRRKSSELSPTITLID
                     EKGSRPVKVRNETVIGKAVSGAELILLPGPATAQMDIARAMAPHVEDGQTVFLPPGTF
                     GSYAMAQVVKPKLMWGETGTLPWLARKRGLSAVAITIRAVRLPTGVFPFEKQNEAIAV
                     IRKAFPSVEPCGNALSGALMNAGPIIHPPLILMNAGPLEASSNYDIHSEGTQPAVRAV
                     TDRLDAERIALREALGFGAPHYPLKDHYTSDRWMYGDAHKKLVQSGDWREPVPLREHR
                     YMTEDVIFGLAFLASVARWARVPAPLAEGLLAIAGGILGKDLSRGERTLEALGLAALS
                     KDELMKRL"
     gene            complement(1589..2746)
                     /locus_tag="VGO02_00400"
     CDS             complement(1589..2746)
                     /locus_tag="VGO02_00400"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEV7476176.1"
                     /translation="MSYRAEIPYGCYWSTPFARWQGAYANLNSLELAAEVARKELERR
                     AIPREAIDHGVLGFSVPQKHSFYGLPWVAGMAGLGAIGGQTVMQACATGVRALLAGAQ
                     EIESGLSQVTLTLTCDRTSNGPHLYYPNPRGPGGTGGHEDWVMDNFSCDPLGPHAMIR
                     TAENVAARHRISTAEQHDVVLRREAQYKEAQGGFHQRFMSRATKLEGDEGIRFSSAEG
                     LAQLKPVLEGGTVTYGGQTHPADGNAAILLAAPQKARELSRDPKLRVRLLGFGQARAA
                     LAQMPEAPLPAAKRALEQAGLDIGAMHAVKTHNPFALNDILFSRQTGVALEKMNNYGC
                     SLVWGHPQGPTGTRSVIELIEELALRGGGYGLFTGCAAGDTAMAVVLKVGE"
     gene            complement(2746..>3316)
                     /locus_tag="VGO02_00405"
     CDS             complement(2746..>3316)
                     /locus_tag="VGO02_00405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HEV7476177.1"
                     /translation="LISGVPGLEKVHIYTPGRAHDPFLNDGAPPPLALQLYFSSLSAL
                     ESAVAGPLSPVKGICQVQAMLVRPFAVAEPRVDPLCTYLVAYEGKAEDENAWHAYYLA
                     HHPPLMAKLPGIRELEVYTPVQWVCPQGWGRAQCMQRNKVAFDSAEALTAALNSPVRK
                     EMRADYAKLPAFSGRVTHYPMATSVISSP"
BASE COUNT          518 a         1142 c         1097 g          559 t
ORIGIN      
        1 gcgcggcacg atgaagccgg gggtcggacc gcagaccacg gggacttttc ctgcctcctc
       61 cagaagcgcc tttaattttt tgattatttc aggggaagtc ctgctgtgaa cggaaatctc
      121 caccagcggt atcagatagg cagggttgag ccagtgcgca ttcagcagcc gctccgggtg
      181 cgtgaccagc ggcaccaggt cggtgaccag gatggtggag gtggtcgagg caagaattgc
      241 atttttggaa ataattccac aaatacgggc gaaggcgtcc cgcttggcct ccagcgtttc
      301 cggcaccgcc tcgaaaacca gctctgcgtc gcgcagcgca gcgggatcct gcaccacgct
      361 tacgtgcgac aggtcgaccc gcgggatcgc gcccagttcg acgagcatgg acaggctcgc
      421 ctccatctcc tccatcgctt cagtcttgag ctggtcgaaa tcagagcgcg ccttgaggtc
      481 gaccagcgtg atgtcgcggc cggcgagcgt gaacgcaaga gcgatgcccc gccccatgcg
      541 ccccgcgccg acgcaagcga tcttcatagc cgtttcatca actcgtcttt ggagagcgca
      601 gcgaggccga gggcttccag cgttctttct ccgcgggaaa gatccttgcc gagtatcccg
      661 cccgctattg ccagcaaccc ctcagcgagc ggcgcgggta cccgcgccca gcgagcaaca
      721 gaggcgagga atgccagccc gaagatcacg tcctccgtca tataccgatg ctcacgcagg
      781 gggaccggct cgcgccagtc gccggactgc accagcttct tgtgcgcgtc accgtacatc
      841 cagcggtcgc tcgtatagtg atccttgagc ggatagtgcg gcgcgccgaa gccgagggct
      901 tcacgcagcg cgatgcgctc ggcgtccagg cggtcggtca ccgcccgcac cgcgggctgc
      961 gtgccctcgc tgtggatgtc gtagttgctg ctcgcctcga gggggccggc gttcatcagg
     1021 atgagcggcg gatggatgat cgggccggcg ttcatcagcg cgccggagag cgcgttgccg
     1081 cagggctcga ccgaggggaa agccttgcgg atcaccgcta tggcttcatt ctgtttttcg
     1141 aaaggaaaaa cgcccgtcgg caggcgcacg gcgcggatgg tgatggcgac cgccgacagg
     1201 ccgcgcttgc gcgcgagcca cggcagcgtg ccggtctcgc cccacatcag cttgggcttc
     1261 accacctgcg ccatggcgta gctgccgaac gtgccgggcg gcaggaacac ggtctgcccg
     1321 tcctccacgt gcggcgccat ggcgcgcgcg atgtccatct gcgccgtggc cggtccgggc
     1381 aggaggatca gctcggcgcc gctcaccgcc ttgccgatga ccgtttcgtt gcgcaccttg
     1441 accggccgcg agcccttctc gtcgatcagc gtgatcgtag gcgagagctc ggagctcttg
     1501 cgccgccagt gtcggatttc gtggccctgc tcggcgaggt cggccgcggc tgcatacgcg
     1561 ccgtgcccgc cgccaagcac ggcgattttc attcgcccac ctttagaacc accgccatcg
     1621 ccgtatcacc agcggcgcag ccggtgaaca gaccgtagcc gccgccgcgc aatgcgagct
     1681 cttcgatcag ctcgatcacc gagcgcgtgc cggtgggccc ctgcgggtgt ccccacacca
     1741 gcgagcagcc gtagttgttc atcttctcca gggcgacgcc ggtctgccgc gagaacagga
     1801 tgtcgttgag cgcgaacggg ttgtgcgtct tcaccgcgtg catcgcaccg atgtcgagcc
     1861 ccgcctgctc gagcgcgcgc ttcgccgcgg gcagcggcgc ttccggcatc tgcgcgagcg
     1921 cggcgcgcgc ctggccgaag ccgagcaggc gcacgcgcag cttcgggtcg cggctcagct
     1981 cgcgcgcctt ctgcggcgcg gcgagcagga tcgcggcgtt gccgtcggcc gggtgcgtct
     2041 gcccgccgta ggtcaccgtg ccgccctcga gcaccggctt gagctgcgcc agcccctcgg
     2101 cgctcgagaa gcggatgccc tcgtcgcctt ccagcttggt ggcgcggctc atgaagcgct
     2161 ggtggaatcc gccctgcgcc tccttgtact gcgcctcgcg ccgcaagacg acgtcgtgct
     2221 gctccgccgt cgagatcctg tgcctcgccg ccacgttctc cgcggtgcgg atcatggcgt
     2281 gcggcccgag cgggtcgcag gagaagttgt ccatcaccca gtcctcgtgg ccgccggtgc
     2341 cgccgggccc gcgcggattc ggatagtaga ggtgcggccc gttggaggtg cggtcgcagg
     2401 tcagcgtgag cgtcacctgc gacaggccgc tctcgatctc ctgcgccccg gcgagcagcg
     2461 cgcgcacgcc ggtggcgcag gcctgcatca cggtctgccc gccgatggcg cccaggccgg
     2521 ccatgccggc gacccacggc agcccgtaga acgagtgctt ctgcggcacg ctgaagccga
     2581 gcacgccgtg gtcgatggct tcgcgcggga tggcgcggcg ctcgagctcc ttgcgcgcca
     2641 cctcggcggc gagctcgagc gagttcagat tggcatacgc accctgccag cgcgcgaagg
     2701 gcgtgctcca gtagcagccg tagggaattt ccgctcggta cgacactacg gcgacgatat
     2761 cacgctcgtc gccatcggat agtgcgtgac gcgcccggag aacgccggca gcttcgcgta
     2821 gtccgcgcgc atctctttgc gcaccggcga attcagcgcc gcggtgagcg cctcggcgct
     2881 gtcgaacgcg accttgttgc gctgcatgca ctgcgcgcgc ccccatccct gcgggcatac
     2941 ccactgcacc ggcgtgtaga cctcgagctc gcggattccc ggcagcttcg ccatcaacgg
     3001 cggatgatgc gcaagatagt acgcatgcca cgcgttctca tcctcggcct ttccctcgta
     3061 tgccacgagg taggtgcaga ggggatccac tctcggctcg gcaaccgcaa acggcctcac
     3121 cagcatggcc tgaacctggc agatcccttt caccggcgaa agcggacccg cgaccgccga
     3181 ttccagcgca gacaacgaac tgaaatagag ctgcagggcg agcggcggcg gcgcgccgtc
     3241 gttcaggaaa gggtcgtgcg ccctgcccgg cgtatagatg tgaaccttct cgaggcccgg
     3301 gacgccgctg atcaga
//
LOCUS       DASYJA010000020         4586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_821333, whole genome shotgun sequence.
ACCESSION   DASYJA010000020 DASYJA010000000
VERSION     DASYJA010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821333"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1878)
                     /locus_tag="VGO02_00410"
     CDS             complement(<1..1878)
                     /locus_tag="VGO02_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEV7476178.1"
                     /translation="MKTSPGDSSLAARLRKEIDGEVLFDAASRGRYSTDASIYQVEPL
                     GVVIPRSEEAARAAISLAAAEGIPILPRGAGSSQCGQAVGAALIIDHTKYLNRIVEEN
                     IQQKVVVVQPGVVLDHLNAALRPHGLWFPVDVSTSAQATLGGMAGNNSCGSRSIAYGN
                     MVHNVLAIDAYTVLGKQWRFGPMGESSGEYGEFVGKLKNLYEREKAEIEARFPKVLRK
                     VAGYNLDHLGPPHANAAHLLVGSEGTLAYSERIHLKLSRIPPQRALGVCHFPKFYTAM
                     QLTQHIVKLGPSAVELVDRTMIGLARDIAAFRKTVDAFIKGEPDAVLLVEFSGEEDQA
                     HKLQELAQLLADLGLPGSVVPITDAKQQRDLWEVRKAGLNIMMSMKGDGKPVSFIEDC
                     AVPLEHLAEYTDRLTRVFEKHGTRGTWYAHASVGTLHVRPVLDMRRDGAEKMRAIAEE
                     ACAMVKEYKGAYSGEHGDGLVRSEWIAPFFGPRLTAALAEIKSWLDPRGLMNPGKIVN
                     PTRMDDRSLFRFKPGYAARVPETALNWGEWGGFDKAVEMCNNNGHCRKFDAGTMCPSY
                     RATLDEVHLTRGRANTLRLALSNQLAFDDAKEALDLCVSCKGCKRECPTGVDMARMKV
                     EY"
     gene            complement(1905..2570)
                     /locus_tag="VGO02_00415"
     CDS             complement(1905..2570)
                     /locus_tag="VGO02_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV7476179.1"
                     /translation="MQAINPINRRPLHEEAVDRLRDLIVQGQLASGSRLNERLLCEQL
                     GISRTPLREAIKLLATEGLVALLPNRGAQVAPLEPGRLAETLEVMAALESLAGELACR
                     NATADCIAEIRALHEEMVGMHARGDLASYFRYNQAIHLKIIEASGNATLGNAYRQLNA
                     NVLRARYMANLSRERWDEAVREHEQILAALEARDVAQLKRLLRDHLAHKLASVSSALV
                     KAA"
     gene            2630..3820
                     /locus_tag="VGO02_00420"
     CDS             2630..3820
                     /locus_tag="VGO02_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEV7476180.1"
                     /translation="MAGRHFLQIPGPTNVPDRVLRAIDFPTMDHRGPNFAALGKACFA
                     GMKRVFKSTQGEVVIYPASGTGAWEAALVNTLSSGDRVLMAETGHFATLWQKLAGKLG
                     LEVEFLPGDWRHGADPEAIEKRLRADADKRIKAVCVVHNETSTGVVSRIGDVRRAIDA
                     AKHPALYMVDTISSLASLDYRHDEWGVDVTVAGSQKGLMLPPGLSFNCISAKALAASK
                     SAKLPRSYWAWDEMLSNGKGGYFPYTPATNLLYGLREALTMLLDEEGLDAVFARHQRH
                     GEAARRAVRAWGLEVLALDPREYSGSLTAVLVPSGHDADRVRKTILEAFDMSLGTGLG
                     KLAGKVFRIGHLGDFNDLTLMGALAGVEMGLALAGVPVKKEGVRAAMEYLSECARAPA
                     RQAA"
     gene            3851..>4586
                     /locus_tag="VGO02_00425"
     CDS             3851..>4586
                     /locus_tag="VGO02_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7476181.1"
                     /translation="MRRESGFFAAAFAGLAFAATSSFAAWEPTKPVEIVVAAGAGGAS
                     DQMARMMQAAIQKNNLMKQPMVVSLKGGASGAEALIYMKSSPGDPHKVLIAYSLIYML
                     PLSAKIPFNWRELSPVSVIALDQFVLWTSATLPYKNVKEFADAAKAANPPFKMGGTGS
                     KREDHVLTVFLEKKTGAKFAYLPYKSGGEAATQLVGNHTQSNVNNPSENLEVWRAGQV
                     RALCVFDKERIQYKNKVTDTQSWNDIP"
BASE COUNT          792 a         1498 c         1485 g          811 t
ORIGIN      
        1 gtactcgacc ttcatccgcg ccatgtcgac gccggtgggg cattcgcgct tacagccctt
       61 gcacgagacg caaaggtcga gtgcttcctt cgcatcgtcg aaggcgagct gattcgacag
      121 cgccagcctc agagtattcg cgcggccgcg cgtgagatgc acttcgtcca gcgttgcgcg
      181 atacgacggg cacatcgtgc cggcgtcgaa cttgcggcaa tggccgttgt tgttgcacat
      241 ctcgacggcc ttgtcgaagc cgccccactc gccccagttc agcgcggttt ccggcacccg
      301 cgccgcatac cccggcttga agcgaaacag cgagcggtcg tccatgcgcg tcgggttcac
      361 gatcttgccc gggttcatca ggccgcgcgg gtccagccag gacttgatct cggcgagcgc
      421 ggcggtgagg cgcggtccga agaaaggggc gatccattcg gagcgcacca acccgtcgcc
      481 gtgctcgccg gaataggcac ccttgtattc cttcaccatc gcgcaggcct cctccgcgat
      541 ggcgcgcatc ttctcggcgc cgtcgcgcct catgtcgagc accgggcgca cgtggagcgt
      601 gcccaccgac gcgtgcgcgt accaggtgcc gcgcgtaccg tgcttctcga atacgcgcgt
      661 caggcggtcg gtgtattcgg cgaggtgctc gagcggcacc gcgcagtcct cgatgaagga
      721 caccggcttg ccgtcgccct tcatggacat catgatgttc aggcccgcct tgcgcacttc
      781 ccacaggtcc ctctgctgct ttgcgtcggt gatcggcacg acgctgccgg gaaggccgag
      841 atccgccagc agctgcgcca gctcctgcag cttgtgcgcc tgatcttcct ccccggaaaa
      901 ttccaccagg aggacggcgt cgggctcgcc cttgatgaag gcgtccaccg tcttgcggaa
      961 ggcggcgatg tcgcgcgcca ggccgatcat ggtgcggtcg accagctcca ccgccgaggg
     1021 cccgagcttc acgatgtgct gcgtcagctg catcgcggta tagaacttcg ggaagtggca
     1081 cacgccgagc gcgcgctgcg gcgggatgcg cgagagcttc aggtgaatgc gctcggaata
     1141 cgccagcgtg ccttccgagc cgaccagcag gtgcgcggcg ttggcgtgcg gcgggccgag
     1201 gtgatcgagg ttgtagcccg ccaccttgcg cagcaccttc ggaaagcgcg cctcgatttc
     1261 ggctttttcc ctctcataga gattcttcaa cttgccgacg aactctccgt actcccccga
     1321 agattccccc atggggccga agcgccattg cttgccgagg acggtgtagg cgtcgatcgc
     1381 gaggacgttg tggaccatgt tgccgtacgc gatcgagcgc gagccgcagg agttgttgcc
     1441 cgccatgccg ccgagggtcg cctgcgcgct ggtcgagacg tcgaccggaa accacaggcc
     1501 gtgcggccgc agcgccgcgt tcagatggtc aagaactaca cctggttgga ccacgacgac
     1561 tttttgctgg atgttttctt caacaattct gttcaggtac ttggtgtggt cgatgatcag
     1621 ggctgcgccc accgcctggc cgcattgcga gctgcccgcg ccgcgcggca ggatcggaat
     1681 tccttcggcc gcggcgagcg aaatcgccgc gcgggccgcc tcttcgctgc gcgggatcac
     1741 cacgccgaga ggctccacct gatagatcga ggcatcggtc gaataacggc cgcggctcgc
     1801 cgcatcgaac agcacttcgc cgtcgatttc cttgcgcagg cgcgcggcga gggaggaatc
     1861 gcccggcgaa gtcttcaccg cccggacggg aaacgaaggt gcgttcaagc ggccttgacg
     1921 agagccgaag agacactcgc cagcttgtgt gcgagatggt cgcgcagcag gcgcttgagc
     1981 tgcgccacgt cgcgcgcttc gagggccgcg aggatctgct cgtgctcccg cactgcttca
     2041 tcccagcgct cgcgtgacag gttcgccatg tagcgcgcgc gcagcacatt cgcattcagt
     2101 tgccggtatg cattgccgag cgtggcgttg ccggaggctt cgatgatctt cagatggatc
     2161 gcctggttgt agcggaaata gctggcgagg tcgccgcgcg cgtgcatgcc gaccatctcc
     2221 tcgtgcaagg cgcggatctc ggcaatgcaa tcggcggtgg cgttccggca ggcaagctcg
     2281 cccgccagcg attcaagggc ggccatgacc tccagcgtct cggcgagccg gccgggctcg
     2341 agcggcgcga cctgcgcgcc acgattaggc agcagcgcga ccaggccttc ggtcgccagc
     2401 agcttgatcg cctcgcgcag cggggtgcgg gagataccga gctgctcgca cagaagccgc
     2461 tcattgagcc gcgacccgga ggcgagttgc ccctgaacaa tcaggtcccg caggcggtcg
     2521 acggcttcct catgtagcgg ccgacggttt atagggttga ttgcttgcat gaacttcatt
     2581 tttgcatgca aaatacaaaa aatccattcc cgtcttcaag aggcgtctta tggcaggcag
     2641 gcactttctc cagattccag gcccgaccaa tgttcccgac cgggtgctgc gcgccatcga
     2701 tttcccgacc atggaccatc gcgggcccaa cttcgccgcc ctcggaaagg cctgtttcgc
     2761 cgggatgaag cgcgtattca agtccacgca gggcgaagtg gtcatttatc ccgcctcggg
     2821 gaccggggct tgggaagcgg cgctggtcaa tacgctttcg agcggcgacc gtgtgctgat
     2881 ggccgagacg ggccacttcg cgacgctctg gcagaagctc gccggcaagc tcggcctgga
     2941 ggtcgagttc ctgcccggcg actggcgcca cggcgccgac cccgaggcga tcgagaagcg
     3001 gctgcgcgcc gacgccgaca agcgcatcaa ggcggtctgc gtggtccaca acgagacctc
     3061 gacgggtgtc gtatcgcgca ttggcgacgt gcgccgcgcg atcgacgccg ccaagcatcc
     3121 cgccctctac atggtcgata ccatctcgtc gctcgcctcg ctcgactacc ggcacgacga
     3181 atggggcgtc gacgtcacgg tcgccggctc gcagaagggc ctgatgctgc cgccaggact
     3241 gtcgttcaac tgcatctcgg ccaaggcgct cgccgcatcg aagtctgcga agctgccgcg
     3301 cagctactgg gcgtgggacg aaatgctttc gaacggcaag ggcggctact tcccgtacac
     3361 gcccgccacg aatctgctct acggcctgcg tgaagcgctg acgatgctcc tcgatgagga
     3421 agggctcgac gcggtgtttg cccgtcacca gcgccacggc gaagcggcgc gccgcgcggt
     3481 gcgcgcctgg ggcctggaag tgctggccct cgatccgcgc gagtactccg gttcgcttac
     3541 cgcggtcctc gttccgtcgg gccacgacgc cgaccgggtg cgcaagacca tcctcgaagc
     3601 gttcgacatg tcactcggca ccggcctggg caagctcgcg ggcaaggtgt tccgcatagg
     3661 gcacctcggc gacttcaacg accttacgct gatgggggcc ctcgccggag tcgaaatggg
     3721 tcttgcgctc gccggcgttc cggtgaaaaa ggaaggggtg cgagcggcaa tggaatatct
     3781 ttcggaatgc gccagagcgc ctgcacgaca ggccgcctag gcaggtcgaa gtcaacatag
     3841 gaggagtgac atgaggagag aatccggctt ttttgccgct gcgttcgctg gtctggcatt
     3901 cgcggcgacg tcgtcgtttg ccgcatggga gccgaccaag ccggtcgaga tcgtcgtcgc
     3961 cgcgggcgcc ggcggagcct ccgaccagat ggcgcggatg atgcaggccg ccatccagaa
     4021 gaacaacctg atgaagcagc cgatggtggt ttcgctcaag ggcggcgcct cgggcgcgga
     4081 agcgctgatc tacatgaaga gcagtcccgg ggacccgcac aaggtgctga tcgcctattc
     4141 gctgatctac atgctgccgc tgtcggccaa gatcccgttc aactggcgcg agctctcgcc
     4201 ggtctcggtc atcgcgctcg accagttcgt gctctggacc agcgcgacgc ttccgtacaa
     4261 gaacgtcaag gaattcgccg acgcggcgaa agcggcgaac ccgccgttca agatgggcgg
     4321 caccggctct aaacgtgagg accatgtcct cacggttttc cttgaaaaaa agaccggcgc
     4381 gaagttcgcc tacctgccgt acaagtccgg cggcgaggcg gcgacgcagc tcgtcggcaa
     4441 ccacacccag tcgaacgtca acaatccttc cgagaacctc gaagtctggc gggcgggcca
     4501 agtgcgcgcc ctttgcgttt tcgacaagga gcgcatccag tacaagaaca aggtcaccga
     4561 cacccagtcg tggaacgaca tcccca
//
LOCUS       DASYJA010000021         2043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_911793, whole genome shotgun sequence.
ACCESSION   DASYJA010000021 DASYJA010000000
VERSION     DASYJA010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911793"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VGO02_00430"
     CDS             <1..294
                     /locus_tag="VGO02_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEV7476182.1"
                     /translation="CGCAFQGRDIADALPGKSELFPDPDEWVNPGDSVVVAPGGKVVA
                     GPLHEERGILYAEIDLERVGMARRSLDVVGHYARPDLFQLHLNSRPQAPLHSA"
     gene            291..869
                     /locus_tag="VGO02_00435"
     CDS             291..869
                     /locus_tag="VGO02_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEV7476183.1"
                     /translation="MSAYHAGSRELQDRFDTRRLADRLAERLSRTEFSAEDKVFIETR
                     IMFFLATADAAGRPDCSYKGGLPGFVRVTGAGELAFPSYDGNGMFRSLGNVRVNPAVG
                     LLFLDFERPRRLRVLGTASASESDPLLGEFAGAQLVVRVRAEAIFPNCPRYLHRMQLV
                     EMSPYAPRPGYEPPVPAWKEQPAFKEVLPRGS"
     gene            927..1289
                     /locus_tag="VGO02_00440"
     CDS             927..1289
                     /locus_tag="VGO02_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476184.1"
                     /translation="MPLDLSFRPEGDFLFCEVSGPYSLTDFLRLADQVRAERVARGAS
                     RVLVDLRGVTGEISSLDRYRLGIYVAEKPNRAKRLAVVLRPESVNWMFENVASNRGLP
                     TCESADPRYAENWLRAAA"
     gene            1343..1828
                     /locus_tag="VGO02_00445"
     CDS             1343..1828
                     /locus_tag="VGO02_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV7476185.1"
                     /translation="MPSPAPVDRESIARSEDHHALRIWLRLLTCTQLIERQVRSRLRE
                     KFATTLPRFDLMAQLERQRDGLKMNELSRMLMVTGGNITAIVDQLEHEGLVERLDDAA
                     DRRAFRIRLTRTGERTFAELARAHVEWVVVLLAGLSRREQEELLKLLAKVKQHAMEVT
                     S"
     gene            1825..>2043
                     /locus_tag="VGO02_00450"
     CDS             1825..>2043
                     /locus_tag="VGO02_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV7476186.1"
                     /translation="MIQPKQFLWRAEGKVGVITLNRPEKKNPLTFESYAELRDLFLDL
                     ANDNSVKSVVVTGAGGNFCSGGDVHEIIG"
BASE COUNT          313 a          704 c          686 g          340 t
ORIGIN      
        1 tgcggctgcg cgttccaggg gcgcgacatc gcggacgcgc tgccggggaa gtcggagctg
       61 tttcccgacc ctgacgagtg ggtcaacccc ggcgattcgg tcgtcgtcgc gccgggcggc
      121 aaggtcgtgg ccggaccgct gcacgaagag cgcggcatcc tgtacgccga aatcgacctc
      181 gagcgtgtcg gcatggcgcg ccgcagcctg gatgtcgtcg gccactacgc gcgccccgac
      241 ctctttcagc tgcacctcaa cagccggccg caagcgccgc tgcattcggc atgagcgcgt
      301 atcacgccgg ctcgcgcgag ctgcaggacc gcttcgacac gcggcgcctg gccgaccggc
      361 tggccgagcg cctgtcgcgc accgagttca gcgccgagga caaggtgttc atcgagacgc
      421 gcatcatgtt cttcctcgcc acggccgacg ccgccggccg gcccgactgc tcctacaagg
      481 gcgggctgcc cggcttcgtg cgcgtgaccg gcgcgggcga gctcgccttc ccgagctacg
      541 acggcaacgg catgttccgc agcctgggca acgtgcgggt caatccggcg gtcgggctgc
      601 tgttcctcga tttcgagcgg ccgcggcgac tgcgggtgct cggcaccgcc agcgcctccg
      661 aaagcgaccc cttgctgggg gagttcgccg gcgcccagct cgtggtgcgc gtgcgggccg
      721 aggcgatttt tccgaactgc ccgcgctacc tgcaccgcat gcagctggtt gaaatgtcgc
      781 cgtacgcgcc gcggccgggg tacgagccgc cggtgccggc atggaaggag cagccggctt
      841 tcaaggaggt gctgccgcgg gggtcttgac agcgatgctg cggtgccgca taatgcttta
      901 agcttcattc attgcgggtt caacctatgc cgctagacct gtctttccgc cctgagggcg
      961 acttcctgtt ctgcgaggta agcgggccct actcgctgac cgacttcctg cgcctggcgg
     1021 accaggtgcg cgccgagcgc gtggcgcgcg gcgccagccg cgtgctcgtc gacctgcgcg
     1081 gcgtgaccgg cgagatctcc tcgctcgacc gctaccgcct gggcatttac gtcgcggaga
     1141 agcccaaccg cgccaagcgc ctcgccgtcg tcctgcggcc cgaatcggtc aactggatgt
     1201 tcgagaacgt cgcctccaac cgcggcctgc cgacctgcga gagcgccgac ccgcgctacg
     1261 ccgagaactg gttgcgcgcc gcagcgtgac aagcggcggg catttccttt aagcttgatc
     1321 tattcaggct tggaggaatc aaatgccatc gcccgccccc gtcgaccgcg agtcgatcgc
     1381 ccgctccgaa gaccaccacg cgctgcgcat ctggctgcgg ctgctcacct gcacgcagtt
     1441 gatcgagcgc caggtgcgct cgcgcctgcg cgagaagttc gccaccacgc tgccgcgctt
     1501 cgacctgatg gcgcagctcg agcggcagcg cgacggcttg aagatgaacg agctgtcgcg
     1561 catgctgatg gtgaccggcg gcaacatcac cgcgatcgtc gaccagctcg agcacgaggg
     1621 cctggtcgag cggctggacg acgccgccga ccggcgcgcc tttcgcatcc gcctgacgcg
     1681 caccggcgag cgcacttttg ccgagttggc gcgcgcgcac gtggaatggg tggtcgtgct
     1741 gctcgccggc ctgtcgcggc gcgagcagga agagctcctc aagctcctcg ccaaagtgaa
     1801 gcagcatgcg atggaggtca cttcatgatc cagccgaagc agttcctctg gcgtgccgag
     1861 ggcaaggtcg gcgtcatcac gctcaaccgc ccggagaaga agaatccgct caccttcgaa
     1921 tcgtatgcgg agctgcgcga cctttttctg gatttggcaa acgacaattc cgtaaagagc
     1981 gtcgtggtga ccggtgcggg cggcaacttc tgctccggcg gcgacgtgca cgagatcatc
     2041 ggc
//
LOCUS       DASYJA010000022         2961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1184815, whole genome shotgun sequence.
ACCESSION   DASYJA010000022 DASYJA010000000
VERSION     DASYJA010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184815"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VGO02_00455"
     CDS             complement(<1..1122)
                     /locus_tag="VGO02_00455"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF005600.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEV7476187.1"
                     /translation="MNPSLEDIRSAARRLEGAIEKTPLVHSRTLSRLTGAEVWLKLEN
                     LQFTASFKERGALNKLLALSEAERRRGVIAMSAGNHAQAVAYHAARLGIPATIVMPRG
                     SPNTKIRNTQVHGATVLLEGDNLFESGKHARALAARDDLVFVHPYEDPLIIAGQGTVG
                     LEMVEAVPDLEVLVVPVGGGGLISGIATAAKGLNPKIEIYGAESANYPSMYQRLNNLP
                     VECGGDTIAEGIAVKDVGSIALSIIKETVKEVLVVKEETIEKAVVALIEIEKTVAEGA
                     GGAGLAALLEHPQHFAGRRVGLPVSGGNIDSRVLASVLMRGLVRDGRLVRLRVTMPDI
                     SGSLAKVATLIAEAGGNIVEVQHQRIFGTSSVRSPEVEFL"
     gene            1146..2606
                     /locus_tag="VGO02_00460"
     CDS             1146..2606
                     /locus_tag="VGO02_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV7476188.1"
                     /translation="MERNPLQFGAGGAEQFASLLERLEAFTRHSPAAMFFKDREGRYR
                     FVNEQFLARFSLRREQVMGRNDAEIFAGSHAAASAAHDAQVLARGVPLTFEESLRRFG
                     GERVSVVVRFPVYDAGGGVAGVGGIATDITERKLAERSLREQQALLAGAQKLAGVGCW
                     EWDPDHGRMTWSEELYRIHGVDPAAWQPGFENYLDRVHPEDRAAVGAALAKALTEGAS
                     FSFEERVVRPDGEVRHLRTRGEAMRDERGRPAKIFGVCLDITEQKMSFGMLRAVSRRL
                     VEAEEAERRRIARELHDRVGQNLSALNMSLNLVAQQAKELPAALSRRLEEAQALVDAT
                     LQSIENLMTDLRPPLLDEHGLGAALAAYAEEFAQRTGVRVEVQGKQETARILKPDAVI
                     ALYRIAQEALSNIAKHSGARSVEMVLEKQPPHMVLVICDDGHGFEPAVAGRGRWGMSA
                     MRERAEAAGGKLTVLSAPGEGAIVRIAVPLPASAAP"
     gene            2603..>2961
                     /locus_tag="VGO02_00465"
     CDS             2603..>2961
                     /locus_tag="VGO02_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7476189.1"
                     /translation="MTIRILIADDHGIVAEGLKSLAEAEPDIEVVGIVADGREAVQHA
                     RESKPDVVVMDMSMPELNGADATRAILQNDPACRVIVLSMYAEREYVRRALKAGAMGY
                     VVKRSAAKELVDAIRAV"
BASE COUNT          438 a         1014 c         1077 g          432 t
ORIGIN      
        1 caggaactcg acctcggggc ttctcaccga ggaagtgccg aagatgcgct ggtgctgcac
       61 ctcgacgatg ttgccgccgg cctcggcgat gagcgtggcc accttcgcca ggctgccgga
      121 gatgtcgggc atggtgacgc gtaggcgcac caggcgcccg tcgcgcacca ggccgcgcat
      181 cagtaccgag gcgagcacgc ggctgtcgat gttgccgccc gacaccggca ggccgacgcg
      241 ccgcccggcg aagtgctgcg gatgctcgag cagcgccgct agcccggcgc cgcccgcgcc
      301 ctcggcgacg gtcttctcga tctcgatcag ggcgacgacc gccttctcga tggtttcttc
      361 cttcaccacg agaacttcct tcacagtttc cttgatgatc gaaagcgcaa tgctgcctac
      421 atcctttacg gcgatgcctt cggcgatggt gtcgccgccg cactcgaccg gcaggttatt
      481 gaggcgctgg tacatggacg ggtagttggc cgattccgcg ccgtagatct cgattttcgg
      541 attcaggccc ttggccgcgg tggcgatgcc ggagatcagg ccgccgccgc ccaccggcac
      601 caccaggacc tcgaggtcgg gcaccgcctc gaccatctcg aggccgacgg tgccctggcc
      661 ggcgatgatc agcgggtcct cgtagggatg cacgaacacc aggtcgtccc tggcggcgag
      721 ggcgcgggcg tgcttgccgg actcgaacag gttgtcgcct tcgagcagca ccgtggcgcc
      781 gtgcacctgc gtgttgcgga tcttggtgtt gggcgagccg cgcggcatga cgatggtcgc
      841 cgggatgccc aggcgcgccg catggtaggc gaccgcctgc gcgtggttgc cggcgctcat
      901 cgcgatgacg ccgcggcggc gctcggcctc ggacagcgcg agcagcttgt tcagcgcgcc
      961 gcgctccttg aacgaggcgg tgaactgcag gttctcgagc ttcagccaga cctcggcgcc
     1021 ggtgaggcgc gacagggtgc ggctgtggac cagcggggtc ttctcgatgg caccctccag
     1081 ccggcgtgcc gcggagcgga tgtcctccaa tgacgggttc atgtttatag tctagagtca
     1141 gcgcaatgga gcgcaacccc ctgcagttcg gcgccggagg ggcggagcaa ttcgccagcc
     1201 tgctcgagcg gctggaggcg ttcacgcgcc acagcccggc ggcgatgttc ttcaaggacc
     1261 gcgagggccg ctaccgcttc gtcaacgagc agttcctcgc gcgcttctcg ctgcggcgcg
     1321 agcaggtgat ggggcgcaac gacgccgaga tcttcgccgg ctcgcacgcc gcggcctcgg
     1381 ccgcgcacga cgcgcaggtg ctggcgcgcg gcgtgccgct caccttcgag gaatcgctgc
     1441 gccgcttcgg cggcgagcgc gtcagcgtgg tggtgcgctt cccggtgtac gacgcgggcg
     1501 gtggcgtggc gggcgtcggc ggcatcgcca ccgacatcac cgagcgcaag ctcgccgagc
     1561 gctcgctgcg cgagcagcag gcgctgctcg ccggggcgca gaagctggcc ggcgtcggct
     1621 gctgggaatg ggacccggac catggccgca tgacctggtc ggaggagctg taccgcatcc
     1681 acggcgtcga cccggccgcc tggcagcccg gcttcgagaa ttacctcgac cgggtgcacc
     1741 ccgaggaccg cgccgcggtc ggcgccgctc tggcgaaggc gctcaccgag ggcgcgtcct
     1801 tcagcttcga ggagcgcgtc gtgcggcccg acggcgaggt gcgccacctg cgcacgcgcg
     1861 gcgaggcgat gcgcgacgag cgcggccggc cggccaagat cttcggcgtc tgcctcgaca
     1921 tcaccgagca gaagatgtcc ttcggcatgc tgcgcgccgt gtcgcggcgg ctggtggagg
     1981 ccgaggaggc ggagcggcgg cgcatcgcgc gcgagctgca cgaccgcgtc ggccagaacc
     2041 tgtcggcgct caacatgagc ctcaacctgg tggcgcagca ggcgaaggag ctgcccgccg
     2101 cgctcagccg ccggctggag gaggcgcagg cgctggtcga cgccacgctg cagtcgatcg
     2161 agaacctgat gaccgacctg cgcccgccgc tgctcgacga gcacggcctg ggcgcggcgc
     2221 tggccgccta cgccgaggaa ttcgcgcagc gtaccggggt gcgcgtcgag gttcagggca
     2281 agcaggaaac ggcgcgcatc ctgaagcccg acgcggtgat cgcgctctac cgcatcgccc
     2341 aggaggcgct cagcaacatc gccaagcact ccggcgcgcg cagcgtcgag atggtgctgg
     2401 agaagcagcc gccccacatg gtgctggtga tctgcgacga cggtcacggc ttcgagccgg
     2461 cggtggcggg ccgcggccgc tggggcatga gcgccatgcg cgagcgcgcc gaggcggccg
     2521 gcggcaagct caccgtgctg agcgcgccgg gcgagggcgc gatcgtgcgc atcgcggtgc
     2581 cgttgccggc ctccgccgcc ccatgaccat aagaatcctc attgccgacg accacggcat
     2641 agtcgccgag ggcctgaaga gcctggccga ggccgagccg gacatcgagg tggtgggcat
     2701 cgtggccgac ggccgcgaag ccgtgcagca cgcacgcgag agtaagcccg acgtggtggt
     2761 gatggacatg tcgatgcccg agctgaacgg cgccgacgcc acgcgcgcca tcctgcagaa
     2821 cgaccccgcc tgccgcgtca tcgtgctgtc gatgtacgcc gagcgcgaat acgtgcgccg
     2881 cgcgctgaag gcgggcgcca tgggctacgt cgtcaagcgc tccgctgcca aggagctggt
     2941 cgacgccatc cgtgccgtgc a
//
LOCUS       DASYJA010000023         5242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_5097, whole genome shotgun sequence.
ACCESSION   DASYJA010000023 DASYJA010000000
VERSION     DASYJA010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5097"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VGO02_00470"
     CDS             <1..283
                     /locus_tag="VGO02_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEV7476190.1"
                     /translation="AQCEIVGISRDSVKSHEGFKAKMKFPFQLLSDADEALCQQFGVM
                     KMKNMYGRKVRGIERSTFVIDDKGALAREWRGVRVPGHVEEVLNFVKAL"
     gene            321..1742
                     /locus_tag="VGO02_00475"
     CDS             321..1742
                     /locus_tag="VGO02_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEV7476191.1"
                     /translation="MTQKPARTQRKPKVKKLFVVDTNVLMHDPTSLFRFEEHDVYLPI
                     GTLEELDANKKGLSDVSRNARQASRFLDEIVSGQDTDIKQGLQLKLKDGQAAKGRLFL
                     QTEAINGELPASLPLGKTDNQILSVVRYLQARDSQREVVLVSKDINMRIKARALGLAA
                     EDYFNDKVLEDADLLYTGVRALPKDFWNSHGRELESWKKDGHTYYRLHGPLVPKLLVN
                     EFVYDESGERPLYALVKEAAGRTAVLETLKDYTHAKNSVWGITARNREQNFALNLLMS
                     PAVDFVTLVGQAGTGKTLLALAAGLTQVLDDKRYSEIIMTRVTVPIGEDIGFLPGTEE
                     EKMLPWMGALEDNLDVLNVSDESGGEWGRAATRDLVRSRIKVKSLNFMRGRTFMNKWL
                     IIDEAQNLTPKQMKTLVTRAGPGTKVVCLGNIAQIDTPYLTEGSSGLTYVVDRMKGWT
                     HAGHVTLARGERSRLADHAAEAL"
     gene            1833..2657
                     /locus_tag="VGO02_00480"
     CDS             1833..2657
                     /locus_tag="VGO02_00480"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7476192.1"
                     /translation="MRTLALLAALLLSFGAAAQPKYEPQVGQAGKDVIWVPTPDDVVD
                     RMLTMAQTGPNDIHFDLGAGDGKIAIAAAKRFGARATGIEYNPDMAKHANANAQAAGV
                     AGVGPGKATIRQGDIFATDFSSATVITLYLLPALNMKLRPTLLQMRPGTRVVSHSFTM
                     EDWEADEVSSMDGRRAYFWVVPANVMGAWQLEAGSQKSEISIDQTFQKISGSVKLGTT
                     LGGLREARLSGASIAFAYVDAAGMRRDFSGRVSGGRMEGSFRDDKGAEGRWSATKK"
     gene            2679..4724
                     /locus_tag="VGO02_00485"
     CDS             2679..4724
                     /locus_tag="VGO02_00485"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEV7476193.1"
                     /translation="MRKPLLLLSVTVLVLVGLYAAAGYWLLPGYVRQRLVAEAERLGY
                     PLRLEAVRVDPFRLNVELKGAELAISDEARVIAPRVRADLKLASLLARDFLPRRVSLS
                     GGVVELADSRIQPLQATLTRQPGGYRIASDGALSLQGDLVLAPLRLDAEVSASAVPLA
                     AAQPWVSRLARLELASGLASARGRVRFDSTGSPRFAYEGTLSLKDVVLRGEEAGAAPL
                     ASWQSLDGTDVALSLAPNRLQVGEALLQGAAGRLVIGENRRTNLARIGKAGKGEGGAA
                     PFPVALRRLTVRGGRLDFTDLSLEPRFATTIRELSGTVSGLGSDGDDEARVELDGRVD
                     RYGSARIRGVINPFAPRTRTSVTMSFRNLEMKSLTPYTAKFAGYRIESGKLSADLRYR
                     VRDSQLVGDNKLVIDSLVLGEHVESAQAPDLPVELAIALLTDARGRIELGLPVRGDLS
                     DPEFSLTQLAGKVLGNLLERIASAPFRLLGSLLGGGSERDLDGILFEPGSARLAPPQQ
                     ENAAALAKALAARPHISLAVRGGYDSEADTAALRGLALRRELVKRSGFRLRSGEDPGS
                     VDPSDPRTRQAMETLYIEHGGTAMRMREIRRAAEGNERRYLRTLGEEISAKMPLPQDA
                     GEALARDRAEAVRAALLVNGVAPERVRIGDLEAATAGENGVRTAVAFDAGQSAGSGR"
     gene            4845..>5242
                     /locus_tag="VGO02_00490"
     CDS             4845..>5242
                     /locus_tag="VGO02_00490"
                     /inference="COORDINATES: protein motif:HMM:NF040071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HEV7476194.1"
                     /translation="MNKLRGFLSALACMLASAMPSGFTYAHDDEGDRHHGDNDFKIEV
                     LSSKPYMVSGGDALVRVTVQDRKVSLGSVRVELNGANVTGAFRADAATRTLTGLVTGL
                     RRGETELSVDAKGKGHGRADADITLTNYPV"
BASE COUNT          895 a         1806 c         1761 g          780 t
ORIGIN      
        1 cgcccaatgc gagatcgtcg gcatttcccg cgactcggtg aagtcccatg agggcttcaa
       61 ggcgaagatg aagtttccgt tccagctgct gtcggacgcc gacgaggcgc tctgccagca
      121 gttcggcgtc atgaagatga agaacatgta cgggagaaag gtccgcggca tcgagcgcag
      181 cacctttgtc atcgacgaca agggagcgct ggcgcgcgaa tggcgcgggg ttcgagtacc
      241 aggccacgtc gaggaggtat tgaactttgt taaagcccta tgatgtcgaa ccggccgcag
      301 cagagttgtc tgaagtttca atgacgcaga agcccgcccg cacgcagcgc aaacccaagg
      361 tcaagaagct gttcgtcgtc gacaccaacg tgctgatgca cgacccgacg agcctgttcc
      421 gcttcgagga gcacgacgtc tacctgccga tcggcacgct ggaggagctc gacgccaaca
      481 agaagggcct gtcggacgtc tcgcgcaacg cgcgccaggc gagccgcttc ctcgacgaga
      541 tcgtctccgg gcaggacact gacatcaagc agggcctgca gctcaagctc aaggacggcc
      601 aggccgcgaa gggccgcctg ttcctgcaga ccgaggcgat caacggcgag ctgcccgcca
      661 gcctgccgct cggcaaaacc gacaaccaga tcctctcggt ggttcggtat ctgcaggcgc
      721 gcgattcgca gcgcgaggtg gtgctggtgt ccaaagacat caacatgcgc atcaaggcgc
      781 gcgccctcgg cctcgccgcc gaggactact tcaacgacaa ggtgctggag gacgccgacc
      841 tcctctacac cggcgtgcgc gccctgccca aggacttctg gaacagccac ggccgcgagc
      901 tcgagtcgtg gaagaaggac ggccacacct actaccgcct gcacggcccg ctggtgccca
      961 agctgctcgt caacgagttc gtctacgacg agtcgggcga gcggccgctg tatgcgctgg
     1021 tcaaggaggc ggccgggcgc acggcggtgc tcgagacgct gaaggactac acccacgcca
     1081 agaactcggt ctgggggatc accgcgcgca accgggagca gaacttcgcg ctcaacctgc
     1141 tgatgagccc ggcggtcgac ttcgtcacgc tggtcggcca ggccggcacc ggcaagacgc
     1201 tgctcgccct cgccgcgggg ctcacccagg tgctcgacga caagcgctac tccgagatca
     1261 tcatgacccg ggtcacggtg ccgatcggcg aggacatcgg cttcctgccc ggcaccgagg
     1321 aggagaagat gctgccctgg atgggggcgc tcgaggacaa cctcgatgtg ctcaacgtct
     1381 ccgacgaatc aggcggcgag tggggccgcg ccgcgactcg cgacctggtg cgctcgcgca
     1441 tcaaggtgaa atcgctcaac ttcatgcgcg ggcgcacgtt catgaacaag tggctgatca
     1501 tcgacgaggc gcagaacctc acgcccaagc agatgaagac gctggtgacg cgcgccgggc
     1561 cggggaccaa ggtggtgtgc ctgggcaaca tcgcgcagat cgacacgccc tatctcaccg
     1621 agggcagctc gggccttacc tacgtggtcg accgcatgaa gggctggacg cacgccggtc
     1681 acgtcacgct ggcgcgcggc gagcgctcgc gcctggcgga ccacgcagcc gaagccctgt
     1741 aaggaataag tcaggcctgg gggtgtttca ccttccggcc tgacctttct gctacatttc
     1801 cggcggactt cctccctgcg gaaaaaatct caatgagaac cctcgcccta ctcgcagcgc
     1861 tgctgctttc ctttggcgcc gccgcgcaac ccaagtacga gccccaggtc ggccaggccg
     1921 gcaaggacgt gatctgggtg ccgacgcccg acgacgtcgt cgaccgcatg ctcaccatgg
     1981 cgcagacggg cccgaacgac atccacttcg acctcggcgc cggcgacggc aagatcgcca
     2041 tcgcggcggc gaagaggttc ggcgcccgcg ccaccggcat cgagtacaac cccgacatgg
     2101 cgaagcacgc caacgccaac gcgcaggccg ccggcgtcgc cggcgtcggc ccgggcaagg
     2161 cgaccatccg ccagggcgac atcttcgcca ccgatttcag ctcggccacc gtcattaccc
     2221 tctatctgct gccggcgctc aacatgaagc tgcgcccgac cctcctgcag atgcgcccgg
     2281 gcacgcgcgt cgtctcgcac tccttcacca tggaggactg ggaggccgac gaggtttcct
     2341 cgatggacgg gcggcgcgcc tacttctggg tggtgccggc gaacgtcatg ggcgcctggc
     2401 agctcgaggc cggcagccag aagagcgaga tctccatcga ccagacgttc cagaagatca
     2461 gcggctcggt gaagctcggc acgacgctcg gcggcctgcg cgaagcgcgc ctgagcggcg
     2521 ccagcatcgc cttcgcctac gtcgacgcgg ccggcatgcg ccgcgacttc tccggccggg
     2581 tcagcggcgg caggatggaa ggttcgttcc gcgacgacaa gggtgccgaa gggcgctgga
     2641 gcgcaaccaa gaaatagtcc cgcggtctat gaaggagcat gcgcaagccg ctgctccttc
     2701 tttccgtcac cgtcctcgtc ctcgtcggcc tttacgccgc cgcgggctat tggctcctcc
     2761 cgggctacgt caggcagcgc ctggtggccg aggcggagcg cctcggctac ccactcaggc
     2821 tcgaagcggt ccgcgtcgac ccgtttcgcc tcaacgtgga gctcaagggc gccgagctcg
     2881 cgatcagcga cgaggcgcgc gtcatcgcgc cgcgcgttcg tgccgatctc aagctggctt
     2941 cgctcctggc aagggacttc ctgccgcggc gcgtgtcgct ttccggaggc gtagtcgagc
     3001 tggcggacag ccgcatccag ccgctccagg ccacgctcac caggcagccg ggcgggtacc
     3061 gcatagcgtc ggacggggcg ctctcgctgc agggcgacct ggttctcgcg cctctgcggc
     3121 tcgacgccga ggtgagcgcc tccgcagtgc cgctcgcggc ggctcagccc tgggtttcgc
     3181 ggctggcgcg cctggagctc gcttcggggc tcgcttcggc gcgcggccgc gttcgattcg
     3241 acagtaccgg gtcgccgcgc ttcgcctatg aaggcacgtt gtcgctgaag gacgtcgtcc
     3301 tgcggggcga ggaagcaggc gctgcgccgc ttgcctcgtg gcagtctctc gacggcacgg
     3361 acgtagcgct gtccctggca cccaaccgcc tgcaagtcgg cgaagcgctg ctgcaaggcg
     3421 cggcgggacg gctggtgatc ggcgagaacc gccgcaccaa cctggcgcgc atcggcaagg
     3481 cgggaaaagg cgagggcggc gcggcgccct tcccggtcgc gctgcgccgg ttgacggtgc
     3541 gcggcggccg gctcgatttc accgacctct cgctcgagcc gcgcttcgcc accaccatcc
     3601 gcgagctgtc cggcacggtg agcggcctgg gcagcgacgg cgatgacgag gcgcgcgtcg
     3661 agctcgacgg ccgcgtcgat cggtatggat cggcgcgcat ccgcggcgtg atcaatccgt
     3721 tcgcgccgcg cacgcgcact tcggtgacca tgagcttccg caacctcgag atgaagagcc
     3781 tgacgccgta caccgccaag ttcgccggct accgcatcga gtcgggcaag ctctcggcgg
     3841 acctgcgcta ccgggtgcgc gacagccagc tggtcggcga caacaagctg gtcatcgaca
     3901 gcctggtgct cggcgagcac gtcgaaagcg cgcaggcgcc cgacctgccg gtcgagctgg
     3961 cgatcgccct gctcaccgac gcgcgcggcc gcatcgagct cggcctgccg gtgcgcggcg
     4021 acctgtcgga ccccgagttc agcctcaccc agctcgccgg caaagtgctc ggcaacctgc
     4081 tcgagcgcat cgcctcggcg ccgttccggc tgctcggctc gctgctcggc ggcggcagcg
     4141 agcgcgacct ggacggcatc ctcttcgagc cgggctcggc gcgcctcgcg ccgccgcagc
     4201 aggagaacgc cgcggcgctt gccaaggcgc tcgccgcccg cccgcacatc tccctcgcgg
     4261 tccgcggcgg ctacgacagc gaagccgaca cggccgcgct gcgcgggctc gcgctgcgcc
     4321 gcgagctggt gaagcgcagc gggttcaggc tgcgctcggg cgaagacccc ggctccgtcg
     4381 acccgtccga cccgcgcaca cgccaggcga tggagacgct gtatatcgag cacggcggga
     4441 cggcaatgcg gatgcgcgag atccgccgcg ctgcggaagg gaacgagcgg cgatacctca
     4501 ggacgctcgg cgaggagata tcggcgaaga tgccgctgcc ccaggacgcg ggtgaggcgc
     4561 tggcgcgcga ccgggccgaa gcggtgcgcg ccgcgttgct tgttaacggc gtcgctccgg
     4621 agcgcgtgcg catcggcgat ctggaggcgg ccactgccgg cgagaacggc gtgcgcaccg
     4681 ccgtcgcctt cgacgccggc cagtcggcgg ggagcggtcg ctaggtcgta gatccattgc
     4741 aggtgttcat cagttgatgc ctatcggtta tgtatccccg tggcgcgagg aaataggatc
     4801 cgttcaccca aaaaccagaa attccctttg tggaggaacg gatcatgaac aagctgcgag
     4861 gtttcctgag cgcgctcgcc tgcatgctgg cgagcgcaat gccttcgggc ttcacttatg
     4921 cacacgacga cgagggcgat cgtcatcacg gcgacaacga cttcaagatc gaggtgctgt
     4981 cgagcaagcc ctacatggtc tccggcggcg acgcgctggt gcgcgtcacc gtgcaggaca
     5041 ggaaggtcag cctcggcagc gtgcgcgtcg agctgaacgg tgccaatgtc accggcgcgt
     5101 tccgagccga cgcggccact cgcacgctca ccggcctggt caccggcctg cgccggggcg
     5161 agaccgagct ctccgtcgac gccaaaggca aaggccatgg acgcgccgac gccgacatca
     5221 cgctcaccaa ctatccggtc ca
//
LOCUS       DASYJA010000024         4368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_95767, whole genome shotgun sequence.
ACCESSION   DASYJA010000024 DASYJA010000000
VERSION     DASYJA010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95767"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1620)
                     /locus_tag="VGO02_00495"
     CDS             complement(13..1620)
                     /locus_tag="VGO02_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476195.1"
                     /translation="MKLPDFSDDASPEFADALGCKEWLQSVPLANVPAAQRELLGELE
                     EFNRFPAKPAERLAVMEALREAVHFVQVEQVKRFSNRALPMTEAEATAFDDSNELWEQ
                     MQVGYWHCLESALAGEGGMRSQAALVCQRVLAYIGVKMFSCYRAYRQAPAREWRALHE
                     AYAHAEKLRVTGDGVKDLMNRDVHDSSPRIAYARAVLMGMCSPNELGQRPLTFVSFLL
                     ERWAAKIEVNESPVSEGEGVAPLVADLESDRCPERVDGAAPGKSLRFLDTRALAKSLR
                     NRVGLLRKGESPAKLALGEDCVQPSCEQQLVHLFRQWCQAKPARAADRKRAAAGAQVC
                     NEFAAIHYYIGGKGFRQPVEQKELTQQEREQIATFGRLSTKEEDDYSNVHGFLVESWQ
                     IEDESAQGLHILRKADTPGKRYAHGQLVAVRPADAKTFMLGQVRWLMAGEGGDLHAGI
                     KLLAGVPGAVAVRPTGVNVQSEKFMQAMSLGAVAAINQPTTLVLPSGWFKPKRVIELA
                     TDTEHNVRLLEVVERGSDFERVTYERA"
     gene            complement(1645..2406)
                     /locus_tag="VGO02_00500"
     CDS             complement(1645..2406)
                     /locus_tag="VGO02_00500"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HEV7476196.1"
                     /translation="MQARVVPALRGVRWLGEGWRMFRAAPLGWLSLVFIYLLGTQVLA
                     RVPLVGVVAALLAVPGLTAGMMGAARAASRGGAPRIAMLFDAFRSGPREQLALGAIYL
                     GCSMLIFGAMSLADPDGALRAALAGRGAPEEPEAADLAAAAAAFAILYAPVMMMFWFA
                     PVLAAWHSAGAAKSLFFSFFACLVNWRAFLAYGAVTALVMVALPLAALSLPLVLLGAE
                     RSMMAPLVLPLLVFMLPTLFASFYASYRDVFGGGE"
     gene            complement(2418..3383)
                     /locus_tag="VGO02_00505"
     CDS             complement(2418..3383)
                     /locus_tag="VGO02_00505"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEV7476197.1"
                     /translation="MSVYTNVSEAELTAWLANYSVGRLVACEPIKAGIENSNYFVTTT
                     QGRYVLTLFERLPAEELPFYLDLMAHLARHGIPCPAPIANLSDRYLQDLKGKPATLVT
                     RLPGTSIERPASAAECAELGMLLGRMHLAGRSFAGFLENPRGAKWWRVAAREVRPFLE
                     KSEYDFLEKEILFQAQHRFPDLPRGPVHADLFRDNALSEGGRISGVIDFYFAGVDRLL
                     YDVAVCANDWCLADLAADPALDAERSRELLAAYHAVRPLKPLEREAWPVMLRAAALRF
                     WLSRLHDFHLPRPGTLVHAHDPEHFRRILASRVAGHDAGGTPWIS"
     gene            complement(3385..3720)
                     /locus_tag="VGO02_00510"
     CDS             complement(3385..3720)
                     /locus_tag="VGO02_00510"
                     /inference="COORDINATES: protein motif:HMM:NF022639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HEV7476198.1"
                     /translation="MRAWCVFLLFLLCTAAAAQSKRPPRLEPLPEPPPPPAMPGGPDE
                     PAVNIPVPQGHQVEEMREGGRVVALKVTPPGGKPYYLIDTTGNGNWMRRDSLDDGVRV
                     PMWPIYTFD"
     gene            complement(3732..>4368)
                     /locus_tag="VGO02_00515"
     CDS             complement(3732..>4368)
                     /locus_tag="VGO02_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEV7476199.1"
                     /translation="MAEFFEATERLAGIRPAVSMFGSARVKEGSAYYKLAEETARLLS
                     DAGFATISGGGPGVMEATNKGAFHGKGLAVGLNIDLPFEQASNKYQHISLRFKHFFAR
                     KVTFARYATAFVVLPGGYGTLDELFEALTLVQTGKTRQMPIILVHEPYWRGLLEWCRE
                     RMAAEGMIDATDLDLLQICNEPRQVVDAIFTFYEKRGFEPSPAEREVMLNL"
BASE COUNT          712 a         1520 c         1516 g          620 t
ORIGIN      
        1 cgtgcaggcc gcctaggcgc gctcgtaggt tacgcgctcg aagtccgagc cgcgctcgac
       61 gacctcgagc agccgtacgt tgtgctcggt gtcggtggcg agctcgatga cccgcttcgg
      121 cttgaaccag cccgagggca ggaccagggt ggtgggctga ttgatcgccg cgaccgcccc
      181 cagcgacatc gcctgcatga acttctcgct ctgcacgttc accccggtgg gccgcaccgc
      241 gacggcgccg ggcaccccgg cgagcagctt gatgccggcg tgcaggtcgc cgccctcgcc
      301 cgccatcagc cagcgcacct ggccgagcat gaaggtcttg gcgtcggccg gccgcaccgc
      361 caccagctgg ccgtgcgcgt agcgcttgcc cggcgtgtcg gccttgcgca ggatgtgcag
      421 cccctgcgcg ctctcgtcct cgatctgcca ggactcgacc agaaagccgt gcacgttgct
      481 gtagtcgtcc tcctccttcg tgctcagccg gccgaaagtc gcgatctgct cgcgctcctg
      541 ctgcgtcagc tccttctgct cgaccggctg gcggaagccc ttgccgccga tgtagtagtg
      601 gatcgcggcg aactcattgc acacctgcgc gccggccgcg gcgcgcttgc gatccgcagc
      661 gcgcgccggc ttcgcctggc accactgccg gaaaaggtgc accagctgct gctcgcagga
      721 gggctgcacg cagtcctcgc cgagggcgag cttggcgggt gattcgccct tgcgcagcag
      781 gccgacgcgg ttgcgcaggc tcttcgccag ggcgcgcgtg tcgagaaagc gcaggctctt
      841 gccgggcgcg gcgccgtcga cgcgctccgg gcagcgatcg ctctccagat cggcgaccag
      901 cggcgccacg ccctcgccct cgctcaccgg agactcgttc acctcgatct tcgcggccca
      961 gcgctccagc aggaaggaga cgaaggtgag cggccgctgc ccgagctcgt tcgggctgca
     1021 catgcccatc agcaccgcgc gcgcataggc gatgcgcggc gagctgtcgt gcacgtcgcg
     1081 gttcatcagg tccttcacgc cgtcgccggt gacgcgcagc ttctcggcgt gcgcgtaagc
     1141 ctcgtgcagc gcgcgccact cgcgggcggg cgcctggcgg taggcgcggt agcaagagaa
     1201 catcttcacg ccgatgtagg cgagcacgcg ctggcagacc agcgccgcct gcgagcgcat
     1261 gccgccctcg ccggcaagcg ccgactcgag gcaatgccaa tagccgacct gcatctgctc
     1321 ccacagctcg ttgctgtcgt cgaaggcggt ggcctcggcc tcggtcatcg gcagggcgcg
     1381 gttgctgaag cgcttgacct gctcgacctg gacgaagtgc accgcctcgc gcagcgcttc
     1441 catgaccgcg aggcgctcgg cgggcttggc cggaaagcgg ttgaattcct ccagctcgcc
     1501 gagcagctcg cgctgcgccg cagggacgtt ggcgagcggc acgctctgca gccattcctt
     1561 gcaccccagc gcgtcggcga actcggggga ggcgtcgtct gaaaagtcgg gaagtttcat
     1621 agccaaaacg cgaatgctac cgcatcattc cccgccgccg aaaacatcgc ggtagctggc
     1681 gtagaagctc gcgaagagcg tcggcagcat gaataccagc agcggcagca ccaggggcgc
     1741 catcatcgag cgctccgccc cgagcagcac gagcggcagc gacagggcgg cgagcggcag
     1801 cgcaaccatg acgagcgccg tcaccgcgcc gtaggcgagg aaggcgcgcc agttgaccag
     1861 gcaagcgaag aagctgaaga acagcgactt ggccgcgccg gccgagtgcc aggcggcgag
     1921 caccggcgcg aaccagaaca tcatcatcac cggggcgtag aggatcgcga acgccgcggc
     1981 ggcggccgcc agatcggccg cttcgggctc ctccggcgcg ccgcggccgg cgagcgcggc
     2041 gcgcagcgcc ccgtcggggt cggccagcga catcgcgccg aagatcagca tcgagcagcc
     2101 gaggtagatc gcgccgagcg cgagctgctc gcgcggcccg ctgcgaaagg cgtcgaacag
     2161 catggcgatg cgcggcgcgc cgccgcgcga ggcggcgcgc gccgccccca tcatgccggc
     2221 agtgagcccc ggcacggcga gaagggcggc gactacgccg accagcggaa cccgcgcgag
     2281 cacctgggta cccagcaggt agatgaaaac cagcgacagc cagccgagcg gcgcggcgcg
     2341 gaacatgcgc cagccttcgc cgagccagcg cacgccgcgc aatgccggga caacgcgcgc
     2401 ctgcatgccg gcctcgctca gctgatccag ggggtgccgc ccgcgtcatg cccggcgacg
     2461 cggctcgcca ggatgcggcg gaaatgctcc gggtcgtggg cgtgcaccag cgtgcccgga
     2521 cgcggcaggt ggaagtcgtg caggcgcgaa agccagaaac gcagcgccgc ggcgcgcagc
     2581 atcaccggcc aggcctcgcg ctcgagcggc ttgagcggcc gcaccgcgtg gtacgcggca
     2641 agcagctcgc ggctgcgctc ggcatcgagg gcgggatcgg cggccaggtc ggcgaggcac
     2701 cagtcgttcg cgcataccgc cacgtcgtac agcaggcggt cgacgccggc gaagtagaag
     2761 tcgatgacgc ccgagatacg cccgccttcg gacagcgcgt tgtcgcggaa caggtccgcg
     2821 tgcaccgggc cgcgcggcag gtcggggaag cgatgctgcg cctggaacag gatttccttc
     2881 tccaggaaat catattcgga tttctccaga aaaggcctga cttcccgcgc cgcgacgcgc
     2941 caccacttgg cgccgcgcgg gttctccaga aacccggcga aggagcgccc cgcaagatgc
     3001 atgcggccca gcagcatgcc gagctccgcg cattccgccg cgctcgccgg gcgctcgatg
     3061 gaggttcccg gcagccgggt caccagcgtc gccggcttgc ccttgagatc ctgcagatag
     3121 cgatcggaga gattcgcgat cggcgccggg cagggaatgc cgtggcgcgc gaggtgcgcc
     3181 atcaggtcga ggtagaaagg cagctcctcg gccggcaggc gctcgaacag cgtcagcacg
     3241 tagcgcccct gcgtcgtggt gacgaagtag ttgctgttct cgatgccggc cttgatcggc
     3301 tcgcaagcga ccaggcgccc gacggagtag ttggcaagcc acgccgtcag ctcggcttcg
     3361 gagacgttgg tgtagaccga caagtcagtc gaaggtgtaa atgggccaca tcggcacacg
     3421 cacgccgtcg tcgagcgaat cgcggcgcat ccagttgcca ttgcccgtgg tgtcgatgag
     3481 gtaatagggc ttgccgcccg gcggcgtgac cttgagcgcc acgacgcgcc cgccctcgcg
     3541 catctcctcg acctgatggc cctgcggcac cgggatattc accgccggct cgtcgggccc
     3601 tccgggcatc gccgggggcg gcggcggctc gggcagcggc tcgagcctgg gcggacgctt
     3661 ggactgcgcg gctgcggcgg tgcaaagcag gaacagaagg aacacgcacc aggcgcgcat
     3721 acttgattct actaaaggtt cagcatcacc tcgcgttccg cgggcgaagg ctcgaagccg
     3781 cgcttctcgt agaacgtgaa gatcgcatcg accacttggc gcggctcgtt gcagatctgc
     3841 agcagatcca ggtcggtcgc atcgatcatg ccttcggcgg ccatgcgctc gcggcaccac
     3901 tcgagcaggc cgcgccagta cggctcgtgc accaggatga tcggcatctg gcgcgtcttg
     3961 ccggtctgca ccagcgtcag cgcctcgaac agctcgtcga gcgtgccgta gccgccgggc
     4021 agcacgacga acgccgtcgc gtagcgcgcg aaggtcacct tgcgcgcgaa gaaatgcttg
     4081 aagcgcaggc tgatgtgctg gtacttgttg gaagcctgct cgaacggcag gtcgatgttc
     4141 agcccgaccg cgagaccctt gccgtggaag gcgcccttgt tggtcgcctc catcacgccc
     4201 gggccgccgc cggagatggt ggcgaagccg gcgtcggaca gcaggcgcgc ggtttcctcg
     4261 gcgagcttgt aatacgccga gccctccttc acgcgcgcgc tgccgaacat ggaaaccgcc
     4321 gggcggatgc cggccaggcg ctcggtcgcc tcgaagaact ctgccata
//
LOCUS       DASYJA010000025         2901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_95828, whole genome shotgun sequence.
ACCESSION   DASYJA010000025 DASYJA010000000
VERSION     DASYJA010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95828"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1657
                     /locus_tag="VGO02_00520"
     CDS             <1..1657
                     /locus_tag="VGO02_00520"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEV7476200.1"
                     /translation="LKRVHAILRRLDDDYCDPLELRSDSALGVPGLVDAVRRGNVLVA
                     NALGSKLLESSTLAGYLPGLAEKLLGEPLKMPSVATWWCGEPAALKEVEANIGRLVIK
                     AAFPQLRVEPMFGEDLDERGRKRVLSMVRARPNDYVAQELVHLSQAPVWDRAHPRRIL
                     ARAIGLRVYACASPNGYVVMPGGLTRVASAADARVISMQRGGSSKDTWVMAAGPVSTF
                     SLLRRAVGPDELVRAGTNLSSRVVENLYWYGRYCERCDGAARLLRIALLHYAEDVPGG
                     DDELAWPVIVGLLRHAGVAVEGVPQDAELAVALRAAITDENLAGLAGNARQLARVAAN
                     LRERLSLDQWRTVNRLTQALTQRKTKKAALLDLLGELDAAIASLTTLAGFALDGMTRD
                     PGWRFLSVGRRIERLQWLCTLLKDALAGPPDMDLTWLLRAADSIITYRARYRARPEWM
                     PAFDLLIRDEANPRSIAFQVHGLYDYTQRLTDLFGTFGDETFGDALASLAGIDPGVDL
                     QPGAPRLARYLDGWRDASFRLAEEVGLRFFSHVGEASRQTFAT"
     gene            1667..2479
                     /locus_tag="VGO02_00525"
     CDS             1667..2479
                     /locus_tag="VGO02_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEV7476201.1"
                     /translation="MKLSVVHETHYRYSSPVPLSHQLLHLTPRELPWQQPVSHRLTVD
                     PQPAESEERDDYFGNRTVRLLLATPHDELLVRAESEVIVQPRSQAGSVGGEPAEFLYE
                     SPHVETWRELTDYAAKSAPQGRPPLEAATDLMRRIHRDFKFDRTATSVSTPLKQVMKQ
                     RSGVCQDFAHLMAGGLRALGIPARYVSGYILTSPPPGRPRLVGADASHAWVSVFSGEW
                     IDLDPTNNRVVDDEYVTLAWGRDFDDVTPMRGVILGGGEQQLAVRVTVTPLA"
     gene            2490..>2901
                     /locus_tag="VGO02_00530"
     CDS             2490..>2901
                     /locus_tag="VGO02_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEV7476202.1"
                     /translation="MADRKFGIETLCLHAGQIPDAATGARAAPIYQTTSFVFDSAEHA
                     ASLFNLQTFGNVYSRISNPTVAVFEERMAAIEGGRSALAAATGQAAEVTAILTLCGAG
                     DHIVSASTLYGGTHTLLSVNLKKLGIETTFVDPDD"
BASE COUNT          414 a         1084 c          989 g          414 t
ORIGIN      
        1 cctgaagcgc gtgcacgcca tcctgcggcg cctggacgac gactactgcg acccgctcga
       61 gctgcgcagc gactccgcgc tcggcgtccc gggcctggtg gacgcggtgc gccgcggcaa
      121 cgtgctggtg gccaacgccc tgggctccaa gctgctcgag tcgagcacgc tggccggcta
      181 cctgccgggc ctggcggaga agctgctcgg cgaaccgctc aagatgcctt ccgtggcgac
      241 ctggtggtgc ggcgagcccg cggcgctgaa ggaagtggag gccaacatcg gccgcctggt
      301 catcaaggcg gcgttcccgc agctgcgcgt cgagccgatg ttcggcgagg acctcgacga
      361 gcgcggcagg aagcgcgtgc tctcgatggt gcgcgcgcgg cccaacgact acgtggcgca
      421 ggagctggtc cacctctcgc aggcgccggt gtgggaccgc gcgcatccgc ggcgcatcct
      481 ggcgcgcgcc atcggcctgc gcgtctacgc ctgcgccagc ccgaacggct acgtcgtcat
      541 gcccggcggc ctgacgcgcg tggcgagcgc ggcggacgcg cgcgtcatct cgatgcagcg
      601 cggcggctcg agcaaggaca cctgggtgat ggccgccggc ccggtcagca ccttcagcct
      661 gctgcgccgc gcggtcgggc ccgacgagct ggtgcgcgcg ggcaccaacc tgtcgagccg
      721 cgtggtggag aacctctact ggtacggccg ctactgcgag cgctgcgacg gcgcggcgcg
      781 cctgctgcgc atcgcgctgc tgcactatgc cgaggacgtc cccggcggcg acgacgagct
      841 cgcctggccg gtaatcgtcg gcctgctgcg ccatgccggc gtggcggtgg agggtgtgcc
      901 gcaggacgcc gagctcgccg tggcgctgcg cgcggcgatc accgacgaga acctggccgg
      961 cctcgccggc aacgcgcgcc agctggcgcg cgtcgcggcg aacctgcgcg agcgcctgtc
     1021 gctcgaccag tggcgcaccg tcaaccgcct gacgcaggcg ctcacgcagc gcaagacgaa
     1081 gaaggcggcg ctgctcgacc tgctcggcga gctcgatgcc gccatcgcct cgctcaccac
     1141 gctggcgggc ttcgcgctcg acggcatgac gcgcgacccc ggctggcgct tcctgtcggt
     1201 cggccggcgc atcgagcgcc tgcaatggct gtgcaccctg ctcaaggacg cgctcgccgg
     1261 gccgcccgac atggacctca cctggctgct gcgcgccgcc gactcgatca tcacctaccg
     1321 cgcccgctac agggcccgcc cggaatggat gccggccttc gacctgctga tccgcgacga
     1381 ggcgaacccg cgctcgatcg ccttccaggt gcacggcctg tacgactaca cgcagcggct
     1441 aaccgacctc ttcggcacgt ttggcgacga gaccttcggc gacgcgctgg cgagcctcgc
     1501 gggaatcgac ccgggggtcg acctgcagcc cggcgcgccg cggctcgccc gctacctcga
     1561 cggctggcgc gacgcctcct tccgcctggc ggaggaggtc ggcctgcgct tcttcagcca
     1621 tgtcggcgag gcgagccgcc agaccttcgc cacataaagc ggccgcatga agctttccgt
     1681 cgtccacgag acgcattacc ggtactccag tccggtgccg ctttcccacc agctcctgca
     1741 cctcacgccg cgcgagctgc cctggcagca gcccgtttcg caccggctca cggtcgatcc
     1801 gcagccagcc gagagcgagg agcgtgacga ctacttcggc aaccgcaccg tgcggctgct
     1861 gctcgccacg ccgcacgacg agctgctggt gcgcgcagaa tccgaggtca tcgtgcagcc
     1921 gcgctcgcag gcgggctcgg tcggcggcga gcccgccgag ttcctctacg agtcgcccca
     1981 cgtcgagacc tggcgcgagc tcaccgacta cgcggcgaag agcgccccgc agggacggcc
     2041 tccgctggag gcggccactg acttgatgcg ccgtatccat agggacttca agttcgaccg
     2101 caccgccacc agcgtgtcca cgccgctcaa gcaggtgatg aagcagcgca gcggcgtctg
     2161 ccaggacttc gcccacctga tggccggggg cctgcgcgcc ctcggcatcc cggcgcgcta
     2221 cgtaagcggc tacatcctca cctcgccgcc gccgggccgg ccgcgcctgg tcggcgccga
     2281 cgcctcgcac gcgtgggtgt cggtgttttc cggcgaatgg atcgacctcg acccgaccaa
     2341 caaccgcgtg gtcgacgacg aatacgtgac cctggcctgg gggcgcgatt tcgacgacgt
     2401 cacgccgatg cgcggggtga tcctcggcgg cggcgagcag cagctcgcgg tccgcgtcac
     2461 cgtcacgccg ctcgcttaga atcgggcgca tggctgaccg caagttcggc atcgagacgc
     2521 tctgcctgca cgccgggcag atccccgatg ccgccaccgg cgcgcgcgcc gcgccgatct
     2581 accagaccac ctcgttcgtg ttcgattcgg ccgagcacgc cgccagcctc tttaacctgc
     2641 agaccttcgg caacgtctat tcgcgcatct ccaacccgac ggtggcggtg ttcgaggagc
     2701 gcatggcggc gatcgagggc gggcgctcgg cgctcgccgc cgccaccggc caggcggcgg
     2761 aagtcaccgc catcctgacg ctgtgcggcg cgggcgacca catcgtctcg gcctcgacgc
     2821 tctacggcgg cacgcatacg ctcctgagcg tcaacctgaa gaagctcggc atcgaaacga
     2881 cattcgtcga tcccgatgat g
//
LOCUS       DASYJA010000026         5118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1821072, whole genome shotgun sequence.
ACCESSION   DASYJA010000026 DASYJA010000000
VERSION     DASYJA010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1821072"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VGO02_00535"
     CDS             complement(<1..347)
                     /locus_tag="VGO02_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476203.1"
                     /translation="MLLAASMICAAPSAFANVITDWDEKGIAAVTAMSSLGGTSPYMA
                     QRTMTMLHAAMFDAVNSIEPRYRPYLVQLPADPATLKEAAAAAAAAAVLGTIDAKTAG
                     EMKAALATYLAPLP"
     gene            604..1578
                     /locus_tag="VGO02_00540"
     CDS             604..1578
                     /locus_tag="VGO02_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEV7476204.1"
                     /translation="MRLALIRLAAAALALGATGTFAQEYPTRNINLLVPFAAGGPTDT
                     VARVVAQAMGKPLGHTIIVENRPSAGGILAPEAVKNAKPDGYTILIHHIGMATIPTLY
                     RQLRFNPLTDFEYIGLINEVPMVIVAKAAMPAKDLKEFLAYIKANKDKVSYANAGVGA
                     ASHLCGMLFMSAIQTDFLTVPYKGTGPAMNDLLGGQVDFMCDQTTSTSSQIKSGKIKV
                     YGITSAQRVSSLPDVPTLQEQGLKGADVGIWHGLYAPKGTPKPVIDKLVSAMQAALKE
                     PEVRQRFTDLGAVTYGPEKQTPAALEAHLKAEIAKWAPLIKKAGQYAD"
     gene            complement(1606..2481)
                     /locus_tag="VGO02_00545"
     CDS             complement(1606..2481)
                     /locus_tag="VGO02_00545"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEV7476205.1"
                     /translation="MEDSRRDLQDSNLNSLVPDVQVDRRGFVAACLAAGFAVTAEPLL
                     AQAIKTPMDGLDGGDSKIGDIPIYYAVPKGKNKRPVVLVVGEIWGNHEHIKDVVRRVA
                     KAGYFAVANEPYFRIGELWKLTEIKEVLAGANKLTDEQAFADLDAVVAWADKNPRANT
                     KKLGITGFCRGGRMVWMYTAHNKKVDAGVAWYGGLSPTPPAQPLTPMDVADKIDRPVL
                     GLYGGADQGIPLERVEMLRAGLKAFGNDKKSQIHVYEGMPHAFNADYRPSYRKEAADD
                     GWKRMLAWFKKNGVA"
     gene            complement(2545..3159)
                     /locus_tag="VGO02_00550"
     CDS             complement(2545..3159)
                     /locus_tag="VGO02_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7476206.1"
                     /translation="MSWLMAAVYDRFMKTSEEACLGKWRAELLADLSGEVLEVGAGTG
                     STLSLYPKAVTRLVMAEPDPHMRRRLREKPRPAGVEVSDASLEKLPFDDRSFDAVVCS
                     LVLCSVRDQKAALAEIARVLRPGGRLVFLEHVAADGKPDRLKWQGRIEPVWKHLMGNC
                     HLTRRTEAAIVAAGLRIERIERESIRKALPIVRPSIRGVARKRA"
     gene            complement(3160..3828)
                     /locus_tag="VGO02_00555"
     CDS             complement(3160..3828)
                     /locus_tag="VGO02_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEV7476207.1"
                     /translation="MITVHHLNNSRSQRILWLLEELGLEYEIKPYQRDKATMLAPPEL
                     RAVHPLGKSPVVSDGDQTLAESGAIIEHLADRYGAGKLAPAFGSVERVRYLYWLHFAE
                     GSAQPPLLLKLLFDRIKSKSPLLVRPVARAIADKALSTFVLPNIERNLDFMESELRKA
                     EWFAGNAFSAADIQMSFPLEAARMRGGLNESRPRLMAWLERIHERPAYNRAIERGGKY
                     DLLR"
     gene            complement(3825..4004)
                     /locus_tag="VGO02_00560"
     CDS             complement(3825..4004)
                     /locus_tag="VGO02_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476208.1"
                     /translation="MRPLPILLLLALAGCTSQQLYDNAQGWREAECNKMIDTPRRERC
                     MKEADRSYDEYRKRP"
     gene            complement(4014..>5118)
                     /locus_tag="VGO02_00565"
     CDS             complement(4014..>5118)
                     /locus_tag="VGO02_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEV7476209.1"
                     /translation="RIWLRAQKLDEAAPFGSGPKATRAAVGHLGYVQIDTIHVIERCH
                     HQILYTRIPEYRRADLHRAQSVDKSVFEYWTHALSYVPTRDYRYFIRDMNQHWRSRSA
                     WFGQVTSGDLRKVLMRIRRNGALTIRDIDDDVLVDKDHEWASRKPSKRALQLAFYRGI
                     VTVSARSGMLKTYELASRHFGWERPPKAAPESEILEYLLERALRSQGIVSVDSACHLD
                     APRKPAMRRLIAARVRRKELVPVELEGAGKLEHWARSEALEAPAGPAGDLVHILSPFD
                     PLIHQRKRLHLFFGYEHRFEAYVPPPKRVFGYFAQPVLVGEEIAAVIDLKTDRERRKL
                     LVRKWTWIGKGARLRRKRQIEEALHRFERFQLA"
BASE COUNT          814 a         1718 c         1680 g          906 t
ORIGIN      
        1 gggagcggcg caaggtaggt cgcgagggcg gctttcattt cacccgcggt tttcgcgtcg
       61 atcgtcccga gcaccgcggc ggcggcggcg gcggcggccg cttccttcaa ggtcgccggg
      121 tccgcaggca gttgcaccag atagggccgg tagcgcggct cgatcgaatt gaccgcgtcg
      181 aacatcgcgg cgtgcagcat cgtcatcgtc cgctgggcca tgtagggcga ggtgccgccg
      241 aggctggaca tcgccgtgac ggcagcgatg cctttctcgt cccaatcggt gatgacgttg
      301 gcgaaagccg atggcgccgc gcagatcatg ctggcggcga gcagcatctt gcgaagattg
      361 gcatgcatgt tgcactccct ggttgtcggc aagccgcttc tgcggatcgc agctgcaagc
      421 ttgtcggcgc acaggtccgg cgtaaatgag accaacggat cgccttccag cctcgctata
      481 atcgcgccgg atagaccaac ccgctgtggt gatgcacttt tttggtttgg ttaggtcctg
      541 ccagacgatc catcggtgct acgactgaag tgccggtact ctagaaacat ataggaggag
      601 caaatgagac ttgccttgat tcgcttggcg gcggctgcgt tggctcttgg cgccacaggc
      661 acattcgcgc aggagtaccc cacccgcaac atcaacctgc tggtgccgtt cgccgccggc
      721 gggccgaccg acacggtggc gcgcgtggtg gcgcaggcca tgggcaagcc cctcggccac
      781 accatcatcg tggagaaccg gccgagcgcc ggcggcatcc tcgcccccga ggcggtgaaa
      841 aacgccaagc ccgacggcta caccatcctg atccaccaca tcggcatggc gaccatcccg
      901 acgctgtacc gccagcttcg cttcaacccg ctcaccgact tcgagtacat cggcctgatc
      961 aacgaagtgc cgatggtgat cgtcgccaag gccgccatgc cggcgaagga cctcaaggaa
     1021 ttcctcgcct acatcaaggc gaacaaggac aaggtgtcct acgccaacgc cggcgtcggc
     1081 gccgcctcgc atctttgcgg catgctcttc atgagcgcga ttcagacgga cttcctcacc
     1141 gtgccgtaca agggcaccgg cccggcgatg aacgacctgc tcggcggcca ggtcgacttc
     1201 atgtgcgacc agaccaccag cacctcctcg cagatcaaga gcggcaagat caaggtctac
     1261 ggcatcactt cggcgcagcg cgtcagttca ctgcccgacg tgcccacgct gcaggagcag
     1321 ggcctcaagg gcgccgacgt cggcatctgg cacggcctgt atgcgccgaa gggcacgccc
     1381 aagccggtga tcgacaagct ggtctccgcg atgcaagcgg cgctgaagga gcccgaggtg
     1441 cgccagcgct tcaccgacct tggcgcggtc acctacgggc cggagaagca gacgccggcc
     1501 gcgctcgagg cgcacctcaa ggcagagatc gcgaagtggg cgccgttaat taaaaaagcc
     1561 ggtcagtacg cagactgacc ggctgtttgc ctgtaaagat cttttctacg cgacgccgtt
     1621 cttcttgaac caggcgagca tccgtttcca gccgtcgtcg gccgcttcct tgcggtagct
     1681 cgggcggtag tcggcgttga aggcgtgcgg catgccttcg tagacgtgga tctgcgattt
     1741 cttgtcgttg ccgaacgcct tgaggccggc gcgcagcatc tcgacgcgct ccagcggaat
     1801 gccctggtcg gcgccgccgt agaggcccag gaccggacgg tcgatcttgt cggcgacatc
     1861 catcggcgtg agcggctgcg ccggcggcgt cgggctcagg ccgccgtacc aggccacgcc
     1921 ggcgtcgacc ttcttgttgt gcgcggtgta catccacacc atgcgcccgc cgcggcagaa
     1981 gccggtgatg ccgagcttct tggtgttggc gcgcggattc ttgtcggccc aggccaccac
     2041 cgcgtcgagg tcggcgaacg cctgctcgtc ggtgagcttg ttcgcgccgg ccagcacctc
     2101 cttgatctcg gtcagcttcc acagctcgcc gatgcggaag taaggctcgt tggccaccgc
     2161 gaagtagccg gccttggcga cgcggcgcac cacgtccttg atgtgctcgt ggttgcccca
     2221 gatctcgccc accaccagga ccaccgggcg cttgttcttg cccttgggca ccgcgtagta
     2281 gatggggatg tcgccgatct tgctgtcgcc gccgtccagg ccgtccatcg gcgtcttgat
     2341 cgcctgcgcg agcagcggct cggcggtgac cgcgaagccg gcggccaggc acgcggcgac
     2401 gaagccgcgg cggtccacct gcacgtcggg caccaggctg ttcagattgc tgtcctgcaa
     2461 atccctgcgc gagtcttcca tctgccttct cctccttgaa ttggtactgg gattacgctt
     2521 tcggcggcat gattattcca ttgctcaggc gcgtttacga gccacgccgc ggatgctggg
     2581 ccttacgatc ggcagcgcct tgcggatgct ttcgcgctcg atgcgctcga tccgcagccc
     2641 cgccgccacg attgccgcct cggtgcgccg cgtcaaatgg cagtttccca tcaagtgctt
     2701 ccatacgggc tcgatgcgcc cctgccactt cagccggtcc ggcttgccgt cggctgcgac
     2761 gtgctcgaga aaaaccagcc tgccgccggg cctgagcacg cgagcgatct ccgccagcgc
     2821 cgccttctgg tcgcgcaccg agcagagcac cagagagcag accacggcgt cgaaggaccg
     2881 gtcgtcgaac ggcagcttct ccagggatgc gtccgacacc tcgacccccg cgggccgggg
     2941 cttctcccgc agcctgcgcc gcatgtgcgg gtcgggctcc gccatcacca ggcgcgtcac
     3001 cgctttcgga tagagagaca aggtcgagcc ggtgccggcg ccgacctcca gcacttcgcc
     3061 ggagagatcg gcgagaagct cggcgcgcca cttgccgagg caagcctcct cgctcgtttt
     3121 catgaagcgg tcgtagaccg cggccatcag ccaggacatc tagcgcagca ggtcgtactt
     3181 gccgccgcgc tcgatcgccc tgttgtacgc ggggcgctcg tgaatccgct ccagccaggc
     3241 catcagccgc ggccggctct cgttcagtcc gccgcgcatg cgcgccgcct cgagcgggaa
     3301 gctcatctgg atatcggcgg cgctgaatgc gttgccggcg aaccactcgg ctttgcgcag
     3361 ctcgctttcc atgaagtcca ggttgcgctc gatattcggc agcacgaacg tgctgagcgc
     3421 cttgtcggcg atggcgcgcg ccaccggccg cacgaggagc ggtgacttgc tcttgatgcg
     3481 gtcgaagagc agcttgagca gcagcggcgg ctgcgccgaa ccttccgcga aatgcagcca
     3541 gtacagatag cgtacgcgct ccacggagcc gaaggccggg gcgagcttgc ccgcgccata
     3601 gcggtcggca aggtgctcga tgatcgcgcc cgactcggcg agcgtctggt cgccgtcgct
     3661 caccaccggc gacttgccca gcgggtgcac cgcgcgcagc tccggcggcg caagcatcgt
     3721 cgccttgtcg cgctggtagg gcttgatctc gtattcgagg ccgagctctt cgagcagcca
     3781 caggatgcgc tgcgagcgcg agttgttcag atggtggacg gtgatcacgg gcgcttcctg
     3841 tattcgtcgt aggagcggtc ggcttccttc atgcagcgct cgcggcgcgg cgtgtcgatc
     3901 atcttgttgc actcggcctc gcgccagccc tgtgcgttgt cgtaaagctg ctgcgacgtg
     3961 cagcccgcaa gcgcgagcag cagcagtatc gggagcgggc gcatcggcgc agtttacgcg
     4021 agctggaagc gctcgaagcg gtgcagcgcc tcctcgatct gccgcttgcg ccggagccgc
     4081 gcgcctttgc cgatccaggt ccacttgcgc accagcagct tgcgccgctc gcggtcggtc
     4141 ttcagatcga tcacggcggc gatctcctcg ccgaccagca ccggctgggc gaagtagccg
     4201 aagacccgct tcggcggcgg aacgtaggcc tcgaagcggt gctcgtagcc gaagaagagg
     4261 tgcaaccgct tgcgctggtg gatgagcggg tcgaagggcg agaggatgtg caccaggtcg
     4321 ccggccggac cggccggcgc ctcgagcgcc tcggagcgcg cccaatgctc gagcttgccg
     4381 gcgccctcga gctcgaccgg caccagctcc ttgcggcgca cccttgccgc gatcaggcgc
     4441 cgcatggccg gcttgcgcgg cgcgtcgaga tggcacgccg aatcgacgct cacgatgcct
     4501 tgcgagcgca gagcgcgctc gagcaggtat tccaggatct cgctctccgg cgcggccttc
     4561 ggcggccgct cccagccgaa atgccggctc gccagctcgt aggtcttcag catgccgctg
     4621 cgcgcgctca ctgtcacgat gccgcgataa aaggcgagct gcagggcgcg cttggacggc
     4681 ttgcggctgg cccactcgtg gtccttgtcg accaggacgt catcgtcgat gtcgcggatg
     4741 gtaagcgcgc cgttgcggcg gatgcgcatc agcaccttgc gcaggtcgcc gctcgtcacc
     4801 tggccgaacc atgcgctgcg gctgcgccag tgctggttca tgtcccggat gaaatagcga
     4861 tagtcgcgcg tcggcacgta cgacagcgca tgggtccagt actcgaagac cgatttgtcg
     4921 acgctctgcg ccctgtgcag gtccgcgcgg cggtattcag ggatgcgcgt gtacaggatc
     4981 tggtggtggc agcgctcgat cacgtggatg gtgtcgatct gcacgtagcc gaggtgcccc
     5041 accgcggcgc gcgtcgcctt cggcccgctg ccgaacggcg cagcctcgtc gagtttttgt
     5101 gcgcgcagcc agattcgg
//
LOCUS       DASYJA010000027         5005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2456612, whole genome shotgun sequence.
ACCESSION   DASYJA010000027 DASYJA010000000
VERSION     DASYJA010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2456612"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VGO02_00570"
     CDS             complement(<1..564)
                     /locus_tag="VGO02_00570"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV7476210.1"
                     /translation="MSPDAIFAMGAAALEAGDFRAAEQLFHQIVNANPRAHPAWNALS
                     VVAVRAGLPEVAAEHVKRALELDRRNPIYLNNLGVVLGELGEFAQAEQAFRRALKAKP
                     AYAEGHFNLGKALHKLGRLDEALRAFERAYAIDADFPGVYASLCQMYRKHGRAERAMT
                     VLRAMSGGLEHSDELSPLVAETLEEIEG"
     gene            complement(575..1774)
                     /gene="tyrS"
                     /locus_tag="VGO02_00575"
     CDS             complement(575..1774)
                     /gene="tyrS"
                     /locus_tag="VGO02_00575"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEV7476211.1"
                     /translation="MTLVQQAIETIRRGCDELIVEAELAKKLATGRKLRIKLGLDPTA
                     PDLHLGHTVVLNKLRDFQQLGHQVQFLIGDFTGMIGDPSGKNQTRPPLTREEILANAQ
                     TYRQQAFKVLDPDQTQVLFNSEWSNKLGAEGMVKLAARYTVARLLERDDFSKRMKNNQ
                     PIAVHELLYPLMQGYDSVAMKADVELGGTDQKFNLLVGRELQKDFGQEPQCILTMPLL
                     EGLDGRDKMSKSLGNYVGIAEPPQEIFGKLMSISDELMWRYIELLSFESQSTIKSWQD
                     EIAKGGNPRDVKVRFAKEIVERFHSADAARQAEESFEKRHRHGEVPEDLSEIAVAVPK
                     EGMLLPQLLKAANLAPSASEASRAIEQGGVRIDGERVAERSLRLLPGRTLVIQVGKRK
                     FARVKLT"
     gene            1875..3104
                     /locus_tag="VGO02_00580"
     CDS             1875..3104
                     /locus_tag="VGO02_00580"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEV7476212.1"
                     /translation="MRHRTAARVVIAVLAATGVVAAFATMAPSPAPFAPNSPLVEALS
                     IRTDALMPAPLTYRREERFQRGDTLAGFLQRLGIAEEDVERLKRVRALQQLRPGMMVT
                     AEVGADGTPHYLNFLNGRDTLVRIGGPLEGYRASEERAQLEARTVMKASVIRSSLFAA
                     SDAAGIPDSIAMQLADVFSGDVDFHRDLRQGDRFSVVYELHHFAGRPVRAGRVLAAEF
                     TNQRKTFRAVYFKGGYYAADGKNLRKAFLRSPLEFSRVTSGFGTRRHPIHKDWRAHKG
                     IDYGAPIGTRVRSVGNGFVDFAGPKGGYGNVVIVRHQGQYSTVYAHLSRIAVRRGARV
                     EQNDTVGYVGQTGWATGPHLHYEFRIAGEARNPFSIAMPAASPLAAQDLPAFEAHAAP
                     LAAQLDLLANGNLAQLE"
     gene            3124..4200
                     /locus_tag="VGO02_00585"
     CDS             3124..4200
                     /locus_tag="VGO02_00585"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEV7476213.1"
                     /translation="MNESLYVGVMSGTSLDGVDAVLLDAAAAPRLLASVHRAFDEPLR
                     RELLALNSPGDNEVDRCARLGNELAKRYAAAVIELLRGCGKDAAQVRAIGCHGQTVRH
                     RPDRGYTLQIGNAALLAELTGVTVVADFRARDIAAGGQGAPLAPAFHATLFRHRSENR
                     VIVNLGGIANVTRLPIEGTVLGYDCGPGNCLLDLWAAKHLGKPYDDNGEWAAGGSVLP
                     ALLARLRDEPFLDAPPPKSTGRDLFNEAWLSRRLRGEEPRAVQATLLEFTARCVADAC
                     QGAARVIVCGGGTSNGALMRRLAALLAPAPVESSAAHGLDPSLVEAAAFAWLAERTLS
                     SAPGNLAEVTGARGPRVLGAIYPK"
     gene            complement(4208..4570)
                     /gene="erpA"
                     /locus_tag="VGO02_00590"
     CDS             complement(4208..4570)
                     /gene="erpA"
                     /locus_tag="VGO02_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003265486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEV7476214.1"
                     /translation="MNAVTEAVTEMPAPLVFSDGAANKVKQLIEEEGNPELKLRVFVT
                     GGGCSGFQYGFTFDEVKNEDDAVMEKNGVQLLIDPMSYQYLVGAEIDYQEGLEGAQFV
                     IKNPNAQSTCGCGSSFST"
     gene            complement(4583..4981)
                     /locus_tag="VGO02_00595"
     CDS             complement(4583..4981)
                     /locus_tag="VGO02_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEV7476215.1"
                     /translation="MFNKASKPQNRIDSLIGATTRIEGNVIFSGGLRVDGMVRGNVAA
                     LPDQPGTLVVSSDARIDGEVQAAHIVVNGTINGPVHATETLELQVGSRVKGDVYYKSI
                     EIHQGAVVEGRLVHHASEVKGVELKLASGG"
BASE COUNT          758 a         1739 c         1628 g          880 t
ORIGIN      
        1 gccctcgatt tcctccagcg tctccgccac caacggggag agctcatcgc tatgctccag
       61 gccacccgac atcgcgcgca acaccgtcat cgcgcgctcc gcgcggccgt gcttgcggta
      121 catctggcac aggcttgcgt acacgcccgg gaaatcggcg tcgatcgcgt aggcgcgctc
      181 gaatgcgcgc agcgcttcat cgaggcggcc gagcttgtgg agggccttgc cgaggttgaa
      241 gtggccttca gcgtacgccg gcttcgcctt gagtgcgcgg cgaaacgcct gctctgcctg
      301 tgcaaactcg ccaagctcgc cgagcaccac gccaagattg ttgaggtaga tgggattgcg
      361 ccggtcgagc tcgagcgcgc gcttcacgtg ctcggcggca acttcgggca gtccggcgcg
      421 caccgccact acggaaaggg cattccacgc cggatgcgca cgcgggttcg cgttcacgat
      481 ctggtggaac agctgctccg ccgcccggaa gtcgcctgcc tcgagcgcgg cggcgcccat
      541 ggcgaagatc gcgtccgggc tcatcgccgc gccgtcaggt cagctttacc cgcgcgaact
      601 tgcgcttacc tacctggatc acgagcgtgc ggcccggcag cagccgcagc gagcgctcgg
      661 cgacccgctc gccgtcgatg cgcacgccgc cctgctcgat ggcacgcgac gcttccgagg
      721 cgctcggcgc gaggttcgcc gccttgagga gctgcggaag cagcatgcct tccttcggca
      781 cggccacggc aatctcggac aggtcctcgg ggacctcgcc gtgacggtgc cgtttctcga
      841 agctttcttc cgcctggcgc gcagcgtccg cggagtggaa gcgctcgacg atctccttgg
      901 caaagcgcac cttgacgtca cgcgggttgc cgcctttggc gatctcgtct tgccagctct
      961 ttatcgtcga ttgcgattcg aacgacagca gctcgatgta gcgccacatc aattcgtcgg
     1021 agatcgacat cagcttgccg aagatctcct gcggcggctc ggcgatgccg acgtagttgc
     1081 cgagcgactt ggacatcttg tcgcggccgt ccagtccctc gagcagcggc atggtgagga
     1141 tgcactgcgg ctcctggccg aagtccttct gcagctcgcg gccgaccagc aggttgaatt
     1201 tctggtcggt gccgccgagc tcgacgtcgg ccttcatcgc caccgagtcg tagccctgca
     1261 tcagcgggta gagcagctca tgcaccgcga tcggctgatt gtttttcatt cttttcgaaa
     1321 aatcatcccg ctcgagcagg cgggcgacgg tatagcgcgc ggccagcttg accatgccct
     1381 cggcgcccag cttgttcgac cactccgagt tgaacaggac ctgggtctgg tccgggtcga
     1441 gcaccttgaa cgcctgctgc cggtaggtct gggcattggc gaggatttcc tcccgggtga
     1501 gaggcggccg cgtctggttc ttgccggagg ggtcgccgat catgccggtg aagtcgccga
     1561 tcaggaactg cacctgatgg ccgagctgct ggaagtcgcg cagcttgttg agcaccacgg
     1621 tgtggccgag gtgcaggtcg ggggccgtgg ggtccagccc gagcttgatg cgcagcttgc
     1681 gccccgtggc gagcttcttg gcgagctccg cctcgacgat cagctcgtcg cacccccggc
     1741 ggatggtctc aatggcctgc tggactaagg tcattcccga ttttcgcccg cggcgatcta
     1801 tgctagcctc cgcaggcttc tgaataatca agagattcta acggaatgtt cttctaaggg
     1861 aatgttctag cgtaatgcgc caccggaccg cagcccgcgt cgtgatcgcc gtcctcgccg
     1921 caaccggcgt ggtcgcggcg ttcgccacca tggcaccctc gcccgcgccg ttcgcaccca
     1981 attccccgct ggtggaggcg ctgtcaatcc ggaccgatgc gctgatgccg gcgccgctca
     2041 cttatcggcg ggaagaacgc ttccagcgcg gcgacaccct ggcgggcttc ctgcagcgcc
     2101 tgggcatcgc cgaagaggac gtcgagcggc tcaagcgggt gcgagcgctg cagcaactgc
     2161 gcccgggcat gatggtgact gccgaagttg gcgccgacgg cacgccgcac tatctgaatt
     2221 tcctcaacgg gcgcgacacg ctggtgcgca tcggcgggcc gctcgaaggc tatcgcgcca
     2281 gcgaagagcg cgcgcagctt gaggcgcgca cggtgatgaa ggcgagcgtg atccgcagct
     2341 cgctgttcgc ggcaagcgat gccgccggca ttcccgacag catcgccatg cagctggccg
     2401 acgtgttcag cggcgacgtc gacttccatc gcgacctgcg ccagggcgac cgcttctcgg
     2461 tggtgtacga gctgcaccat ttcgccggcc ggccggtgcg cgccggacgc gtgctcgccg
     2521 ccgaattcac caaccagcgc aaaaccttcc gcgccgttta cttcaagggt ggctactacg
     2581 cagccgacgg caagaacttg cgcaaggcgt tcctgcgctc gccgctcgag ttctcgcgcg
     2641 tcacctcggg tttcggcacc cgccgccatc cgatccacaa ggactggcgc gcgcacaagg
     2701 gcatcgacta tggcgctccg atcggcacgc gcgtgcgcag cgtcggcaac ggcttcgtcg
     2761 acttcgccgg cccgaagggc ggctacggca acgtcgtcat cgtgcgccac cagggccaat
     2821 actcgacggt ctatgcgcac ctgagccgta tcgcggtgcg ccgcggcgcg cgcgtcgagc
     2881 agaacgacac cgtcggctac gtcggccaga ccggctgggc caccggaccg cacctgcact
     2941 acgaattccg tatcgccggg gaggcgcgca acccattctc catcgcgatg ccggcggcct
     3001 cgccgctcgc cgcgcaggac ctgcccgcct tcgaggcgca cgccgccccg ctcgcggcgc
     3061 agctcgacct gctagccaac ggcaacctcg cgcagctgga gtgagcgact cgccgccagc
     3121 agagtgaacg agtcactcta cgtcggcgtc atgtcgggca ccagcctgga cggggtcgac
     3181 gcggtgctcc tcgacgcggc cgcggcgcca cgcctgctgg caagtgtcca ccgcgccttc
     3241 gacgagccgc tgcgccgcga gctgctcgcg ctcaattctc caggggacaa cgaggtcgac
     3301 cgctgcgcgc gcctgggtaa cgagctggcg aagcgctacg ccgccgccgt catcgagctg
     3361 ctgcgcggtt gcggcaagga tgcggcacag gtgcgcgcca tcggctgcca cgggcagacc
     3421 gtgcgccacc gccccgaccg cggctacacc ctgcagatcg gcaacgccgc gctgctggcc
     3481 gagctgaccg gggtcactgt ggtggcggat ttccgcgccc gcgacattgc cgccggcggc
     3541 cagggcgcgc cgcttgcccc ggcgttccat gcgacgctct tccgccaccg cagcgagaac
     3601 cgcgtcatcg tcaatctcgg cggcatcgcc aacgttaccc ggctgccgat cgaggggacc
     3661 gtcctcggct acgactgcgg tccgggcaac tgcctgctcg atctctgggc ggcgaagcac
     3721 ctcggcaagc cgtacgacga caacggcgag tgggcggccg gcggcagcgt gctgccagcg
     3781 ctgctcgccc ggctgcgcga cgagcctttc ctcgacgccc cgccgccgaa gagcaccggc
     3841 cgcgatctgt tcaacgaagc ctggctgagc cgccggctgc gcggcgagga gccgcgcgcc
     3901 gtgcaggcga cgctgctgga attcaccgcg cgctgcgtcg ccgatgcgtg ccagggtgct
     3961 gcgcgggtca tcgtgtgcgg cggcggcacc agcaacggcg cgctgatgcg acgccttgcc
     4021 gcgctgctgg ctcccgcgcc ggtggaatcg agcgcggcgc acggcctgga cccttcattg
     4081 gtcgaggccg ccgcgttcgc ctggctcgcc gagcgcacgc tgagcagcgc gccgggcaat
     4141 ctcgccgagg tcaccggcgc gcgcgggccg cgcgtgctgg gcgcgatcta tccgaagtaa
     4201 aagggaatta agttgagaaa gaagacccgc aaccgcaggt gctctgcgcg ttggggttct
     4261 tgatcacgaa ctgcgcgcct tcgaggcctt cctggtagtc gatctcggcg cccaccaggt
     4321 actggtagct catcggatcg atcagcagct ggacgccgtt tttctccatc actgcgtcgt
     4381 cctcgttctt cacctcgtcg aaggtgaagc cgtactggaa gcccgagcag ccgccgccgg
     4441 tgacgaacac gcgcagcttg agctccgggt tgccctcttc ctcgatcagt tgcttgacct
     4501 tgttggccgc tccgtcgctg aagaccagcg gcgccggcat ctcggtgact gcttcggtaa
     4561 ctgcgttcat ccgtgttttc ctttacccgc ccgatgcgag cttcagttcc acgcccttca
     4621 cttcggaggc gtggtgcaca aggcgccctt caaccacggc gccctgatgg atttcgatgc
     4681 ttttgtaata cacatcccct ttgaccctgg atccgacctg gagttcgagg gtctcggtgg
     4741 catgcaccgg cccattgatg gtgccgttca ccacgatgtg cgcggcctgc acttcgccgt
     4801 cgatgcgcgc gtcggaggac accaccaggg tgcccggctg gtcgggcagc gccgcgacgt
     4861 tgccgcgcac catgccgtcg acccggagcc cgccgctgaa aatgacgttg ccctcgatgc
     4921 gcgtcgtggc accgatcagg ctatcgatcc ggttctgcgg tttgcttgcc ttgttaaaca
     4981 tggtcatcgc cccttatccg agggt
//
LOCUS       DASYJA010000028         3253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2729101, whole genome shotgun sequence.
ACCESSION   DASYJA010000028 DASYJA010000000
VERSION     DASYJA010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2729101"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /gene="dctP"
                     /locus_tag="VGO02_00600"
     CDS             <1..498
                     /gene="dctP"
                     /locus_tag="VGO02_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEV7476216.1"
                     /translation="DLTGMKIRITPVYREFFQSMNAQVMTTAPGEVYTALERGVIDGY
                     GWPIHALFDLNWHEKTKFRVDPGFYNAEVGLIMNLDKYKSLTPAQRDYLERKAQGYEA
                     QNDFWKSYNQNEAKRQAEAGIQVISFDAATSKAYVEKSKEIGWANAIKASPQYGEQLK
                     KVLAK"
     gene            514..1041
                     /locus_tag="VGO02_00605"
     CDS             514..1041
                     /locus_tag="VGO02_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEV7476217.1"
                     /translation="MEQLEAAYGKLLESLALVACALILGMTLMICADVLLRNVRIIPG
                     VAGLEWSNEISEGMLYVITMLTAPWLLRRGQHIRVDIVLRAVPKHVGWVFEWIVDGLG
                     LACCLAIAYYGARAALASFQAGSISIKTLVTPEWWLLGVLPVAFLALSFEMLFRMRRL
                     ALGERAPRDDAVSTG"
     gene            1073..2359
                     /locus_tag="VGO02_00610"
     CDS             1073..2359
                     /locus_tag="VGO02_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEV7476218.1"
                     /translation="MLGGSTVLLFLGLPVAFSFLVINLVGAWLFLGGEAGLTQFARNS
                     VGSVASFSLTPIPLFILMGEVLFHTGLAVKVIDGVERLIREVPGRLAVVAVVAGTVFS
                     AISGSTIATTAMLGSLMLPVMLSRGYHPTMATGPLMAIGAVDMLIPPSALTVLLGSLS
                     GISISKLLIGGVVPGLLLSLAFVVYIVTRVKLNPALAPASSFVEYHGWQRLRPFVQYV
                     IPLVSIFAIVVMSMSAGWATPTESAAIGAFATMVLAVMYGSLTAKNLLAALRGTAAIS
                     GMILFIIVGATTFSQVLSFSGASNGLVELIAGLGLSPLAVIAAMMLLLIFLGLFVEQV
                     SMMLITLPIYMPLVQKYGVDPVWFGVMFLICMQLGLLLPPHGLLLMTMKGVAPPQVRM
                     AHIFQAVVPYIAMSLLLLALVFFVPAVAVWLPKVLL"
     gene            2362..>3253
                     /locus_tag="VGO02_00615"
     CDS             2362..>3253
                     /locus_tag="VGO02_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase"
                     /protein_id="HEV7476219.1"
                     /translation="MERSFKEEVAKLTLGDGDTFHGEAILAVTKALLQSGVAYVGGYQ
                     GAPVSHVMDVLNDARDILDGLGVHVETNANEAGAAAMLGASINYPMRGAVAFKSTVGT
                     NVASDALSNLASAGVRGGTLLVLGEDYGEGASIIQERTHAFAMKLQMWLLDPRPNLPK
                     IVEMVEQGFELSEASSTPVMLELRIRACHVHGAFEAKDNLAPPFSRRNTMEDPEFDFA
                     RISLPPATYAQEKHKVEVRWPAAVKFIRENRLNEVFEGAVADLGIVCQGGMYNAIVRT
                     LQQLGLADTFGNSRIPIYVLN"
BASE COUNT          517 a         1084 c         1056 g          596 t
ORIGIN      
        1 gacctgaccg gcatgaagat ccgcatcacg ccggtgtacc gcgaattctt ccagtcgatg
       61 aacgcgcagg tgatgaccac cgcccccggc gaggtttaca ccgcgctcga gcgcggcgtg
      121 atcgacggct acggctggcc gatccatgcg ctgttcgacc tcaactggca cgagaagacc
      181 aagttccgcg tcgaccccgg cttctacaac gcggaggtcg ggctcatcat gaacctcgac
      241 aagtacaagt cgctgacgcc cgcgcagcgc gactacctcg agcgcaaggc gcagggctac
      301 gaagcgcaga acgacttctg gaagtcctac aaccagaacg aggccaagcg ccaggccgag
      361 gccggcatcc aggtgatcag cttcgacgcc gcgacgagca aggcgtacgt cgagaagtcg
      421 aaggagatcg gctgggcgaa cgcgatcaag gcgagccccc agtacggcga gcaactgaag
      481 aaagtcctgg ccaaatagga aaacgaagcg cgcatggaac aactcgaggc ggcatacggc
      541 aaattgctgg agtcactcgc cctcgtcgcc tgcgcgctga tcctgggcat gacgctgatg
      601 atctgcgccg acgtgctgct gcgcaacgtg cgcatcatcc cgggggttgc cgggctggag
      661 tggagcaacg agatctccga agggatgctc tacgtcatca ccatgctcac cgcgccctgg
      721 ctgctgcgcc gcgggcagca catccgggtg gacatcgtcc tgcgcgccgt gccgaagcac
      781 gtcggctggg tcttcgagtg gatcgtcgac ggcctcggcc tcgcctgctg cctcgccatc
      841 gcctactacg gcgcccgcgc cgcgctcgcc agcttccagg cgggctcgat ctcgatcaag
      901 acgctggtga cgccggagtg gtggctgctc ggcgtgctgc cggtcgcctt cctcgcgctg
      961 tccttcgaga tgctgttccg catgcgccgc ctggctctgg gcgagcgcgc accgcgcgac
     1021 gacgcggtgt cgaccggcta gagccatgtc atgggacgcg gctgcatggc tgatgctcgg
     1081 cggatccacg gtcctgctgt tcctcggcct gccggtggcg ttctcgttcc tggtcatcaa
     1141 cctggtggga gcgtggctgt tcctcggcgg cgaagccggg ctgacgcagt tcgcgcgcaa
     1201 cagcgtcggc tcggtggcga gcttctcgct cacgccgatc ccgctgttca tcctgatggg
     1261 cgaggtgctg ttccataccg gcctggcggt caaggtgatc gacggcgtcg agcggctgat
     1321 ccgcgaggtg cccgggcgcc tcgcggtggt ggcggtcgtg gcgggcactg tgttctcggc
     1381 gatttccggc tcgaccatcg ccaccaccgc gatgctcggc agcctgatgc tgccggtgat
     1441 gctgtcgcgc ggctaccatc cgaccatggc caccggcccc ctcatggcga tcggcgcggt
     1501 cgacatgctc attccaccct cggccctgac cgtgctgctc ggcagcctgt cgggcatctc
     1561 gatctcgaag ctcctcatcg gcggcgtggt gccggggctg ctgctcagcc tcgcgttcgt
     1621 ggtctacatc gtgacgcgcg tcaagctcaa cccggcgctc gcgccggcgt cgagcttcgt
     1681 cgaataccac ggctggcagc ggctgcgccc gtttgtacag tacgtgatac cgctggtgtc
     1741 gatcttcgcc atcgtggtga tgtcgatgtc cgcgggctgg gcaacgccga ccgagtcggc
     1801 cgcgatcggc gccttcgcga ccatggttct tgctgtgatg tatggttctt taactgcaaa
     1861 aaacctgctt gcagcgcttc gcggcaccgc cgcgatctcg gggatgatcc tgttcatcat
     1921 cgtcggcgcc accaccttct cgcaggtgct gtcgttctcg ggggcgagca acggcctggt
     1981 cgagctgatc gccggcctgg ggctgtcgcc gctcgcggtc atcgcggcga tgatgctgct
     2041 gctgatcttc ctcggcctgt tcgtcgagca ggtgagcatg atgctcatca ccctgccgat
     2101 ctacatgccg ctggtgcaga agtacggcgt cgacccggtg tggttcggcg tcatgttcct
     2161 catctgcatg cagctcggcc tgctgctgcc gccgcacggc ctgctgctga tgaccatgaa
     2221 gggcgtggcg ccgccgcagg tgcgcatggc gcacatcttc caggcggtgg tgccgtatat
     2281 cgcgatgagc ctgctgctcc tcgcgctggt gttcttcgtt ccggcggtcg ccgtgtggct
     2341 gcctaaggtc ctcctgtagc catggaaaga tccttcaagg aagaagtcgc aaaattgacg
     2401 ctgggcgacg gcgacacctt ccacggcgag gcgatcctcg ccgtcaccaa ggcgctgctg
     2461 cagtcggggg tcgcctacgt aggcggctat cagggcgcgc cggtatcgca cgtcatggac
     2521 gtcctcaatg acgcgcgcga catcctcgac ggcctcgggg tgcacgtcga gaccaacgcc
     2581 aacgaggccg gcgccgcagc catgctcggc gcctcgatca actaccccat gcgcggcgcg
     2641 gtcgcgttca agtccaccgt cggcaccaac gtcgcctccg acgcgctttc caacctcgcc
     2701 tcggccggcg tgcgcggcgg cacgctgctc gtgctcggcg aggactacgg cgagggcgcc
     2761 tcgatcatcc aggagcgcac ccacgcgttc gccatgaagt tgcagatgtg gctcctcgat
     2821 ccacggccca acctgccgaa gatcgtcgag atggtcgagc agggcttcga gctgtcggaa
     2881 gcctccagca cgccggtgat gctcgagctg cgcatccgcg cctgccacgt gcacggcgcc
     2941 ttcgaggcga aggacaacct tgcgccgccg ttctcgcgca ggaacacgat ggaagatccc
     3001 gaattcgatt tcgcccgcat ctcgcttccg ccagcgactt acgcgcagga gaagcacaag
     3061 gtcgaggtgc gctggccggc ggcggtgaaa ttcatccgcg agaaccggct gaacgaggtc
     3121 ttcgagggag cggtcgcaga cttgggcatc gtctgccagg gcggcatgta caacgccatt
     3181 gtgcgcacgc tgcagcagct cggccttgcg gataccttcg gaaattccag aatcccgatc
     3241 tacgtcctca acg
//
LOCUS       DASYJA010000029         2104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3092184, whole genome shotgun sequence.
ACCESSION   DASYJA010000029 DASYJA010000000
VERSION     DASYJA010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3092184"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VGO02_00620"
     CDS             complement(<1..370)
                     /locus_tag="VGO02_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEV7476220.1"
                     /translation="MKRRAFIIATAAALVAPALAQNKPMKIGVIGSGRIGSTIGGLWV
                     KAGHEVMFSDRDPEQVKRATDGLGPRARAGSVVEAVAFGDAILIAVPYMALPAIQQQV
                     GEQLKGKVVIDPNNPVASRDG"
     gene            404..1261
                     /locus_tag="VGO02_00625"
     CDS             404..1261
                     /locus_tag="VGO02_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV7476221.1"
                     /translation="MDSRRRTILAAGLGLLMKDSFSKEISLPRIGLGTWQTFDVGTDS
                     AARAPLRELLKLLDGNVIDSSPMYGSSETVAGDLIAELGLRRKLFIATKVWTSGRDEG
                     LRQIETSFKRLRVEVMDLMQIHTLVDAATHTKTLLELKNQKRIRYMGITHYTSSAYLD
                     VERWLGTGQYDFLQINYSLAEREAENRILGLAKDKGVSVLINRPFAEGALFRRTKGKP
                     LPAWAGELGIASWAQYFLKWIVSHPAVTCAIPGTARPEHMKDNLGAGQPPFPSQEQRR
                     KMAAYFDSL"
     gene            1310..>2104
                     /locus_tag="VGO02_00630"
     CDS             1310..>2104
                     /locus_tag="VGO02_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HEV7476222.1"
                     /translation="MRKALFGLLAVAPFALAQVPEIQFDSTPDPLKLPDGMYFGELTG
                     VAVNSKGHVFALSRGNTSGPAYAAAATQLLEFDQTGKFIREIGKNLYAWSYGHTVRVD
                     PQDNIWVTDKGSDMVVKFNPQGRVLMVFGRKQEASDRETGPLERHKPPLPPDVGRFRQ
                     VTDVAWDPQGNAYISDGYINSRVAKVDKLGSWIKSFGEPGSGPGQLNTPHSIATDAKG
                     NVYVADRGNRRIQVFDGEGNVLREIRIDVPFPADAKPALGATPNVEK"
BASE COUNT          396 a          705 c          663 g          340 t
ORIGIN      
        1 cgccgtcgcg cgaagccacg ggattgttgg gatcgatgac gaccttgccc ttcagctgct
       61 cgccgacctg ctgctggatg gcgggcagcg ccatgtaggg caccgcgatc aggatcgcgt
      121 cgccgaaagc caccgcctcg acgacactgc cggcgcgtgc gcgcgggccg aggccgtcgg
      181 tggcgcgctt cacctgctcg ggatcgcggt cggagaacat gacctcgtgg cccgccttca
      241 cccacaggcc gccgatggtc gagccgatcc ggcccgagcc gatgacgccg atcttcatcg
      301 gcttgttctg cgccaaggcc ggcgcgacca gcgccgccgc cgttgcgatt atgaatgcgc
      361 gtcttttcat ggtgggtcag gctatcacag ctaaaatcgc ttcatggact cgcgcagaag
      421 aaccattctt gccgcgggtc tggggctcct catgaaagac tccttttcaa aagaaatttc
      481 cctgccacgc atcggcctgg gcacctggca gaccttcgac gtcggcaccg acagcgccgc
      541 gcgcgcgccg ctgcgcgagc tgctgaagct gctcgacggc aacgtcatcg actcctcgcc
      601 gatgtacggc agctccgaga cggtggcggg cgacctgatc gccgagctcg gcctgcgcag
      661 gaagctgttc atcgccacca aggtctggac cagcggccgc gacgaaggcc tgcggcagat
      721 cgagacctcg ttcaagcgcc tgcgcgtcga ggtgatggac ctgatgcaga tccacaccct
      781 ggtcgatgcg gcaacgcata ccaagacgct tctcgaattg aagaatcaaa aaagaatccg
      841 ttacatgggc attacccact acacctcttc ggcctacctc gacgtcgagc gctggctcgg
      901 caccgggcag tacgacttcc tgcagatcaa ctactcgctc gccgaacggg aagcagaaaa
      961 cagaatttta ggtttggcta aggacaaagg cgtgtcggtc ctcatcaacc ggcctttcgc
     1021 cgagggcgcg ctcttccggc gcacgaaagg caagccgctc cccgcctggg cgggcgagct
     1081 cggcatcgcg agctgggcgc agtacttcct gaaatggatc gtctcgcacc cggcggtgac
     1141 ctgcgcgatc cccggcaccg cgcggcccga gcacatgaag gacaacctcg gcgccggtca
     1201 gccgcctttc ccgagccagg agcaaagaag aaaaatggcg gcctacttcg actcgctcta
     1261 agccagtcgc cctgatggcg cgtaggatgg agatagagga ggttttcgga tgagaaaagc
     1321 cctttttggt ctgctcgctg tcgcaccctt cgctctcgcc caagtcccgg aaatccagtt
     1381 cgattcgacc cccgatccgc tgaagctgcc ggatggcatg tacttcggcg agctgaccgg
     1441 ggtcgcggtc aactcgaaag gccacgtgtt cgcgctgtcg cgcggcaaca cgagcgggcc
     1501 ggcgtacgcc gcggcggcga cgcagctcct cgagttcgac cagaccggca agttcatccg
     1561 cgagatcggc aagaacctgt acgcctggtc ctacggccac acggtgcgcg tcgatcccca
     1621 ggacaacatc tgggtgaccg acaagggctc ggatatggtg gtgaagttca acccgcaggg
     1681 ccgcgtgctg atggtgttcg gccgcaagca ggaggcctcg gacagggaga ccggcccgct
     1741 cgagcgccac aagccgccct tgccgcccga cgtcggccgc ttccgccagg tcaccgacgt
     1801 ggcgtgggat ccgcagggca acgcctacat cagcgacggc tacatcaact cgcgcgtcgc
     1861 caaggtcgac aagctcggca gctggatcaa gtccttcggg gagccgggct cgggtcccgg
     1921 ccagctcaac acgccgcaca gcatcgccac tgacgcgaaa ggcaacgtct acgtcgccga
     1981 ccgcggcaac cgccgcatcc aggtgttcga cggcgagggc aacgtgctgc gcgagatccg
     2041 catcgacgtg ccgttcccgg cggatgcgaa gccggcgctc ggcgcgacgc cgaacgtgga
     2101 gaag
//
LOCUS       DASYJA010000030         6036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_823167, whole genome shotgun sequence.
ACCESSION   DASYJA010000030 DASYJA010000000
VERSION     DASYJA010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823167"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3009)
                     /locus_tag="VGO02_00635"
     CDS             complement(<1..3009)
                     /locus_tag="VGO02_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7476223.1"
                     /translation="MNNPRRMRFWKDWGLVSRLMLAVGIAIITGGGVQTALLVAEGAG
                     EHSARLLREQKETLAFLAPLVADQALVGEYEAISQLLKNQVKKGEVDRFAWTDRDGKT
                     LVAQDAPDKLDAPTWFARLVAIEHQDQSIDVTAGGVGYGTLSASMTPVRAQNRLWAQF
                     VKQLQIVAVTLFLMLQFIWLIFRGNLGTLRMLAEGANRFSQGDHAVRIEPEGAPEVSV
                     AAEAFNNMANSIEGLIASLGKSESKNKLLATIVEQSSEAIWTKDLAGNITSWNSGAAA
                     MFGYTPTEAIGRALNVAESTPQEEAARMQRLMTGDKFSYDAKATTRSGTPIDIQVAVA
                     PLLDDANQCIGSIAVARDVTQHKRSEEALRMAREAAEAANHAKSSFLARMSHEIRTPM
                     NGVLGMTELLLETGLTSNQRKYAETVQRSGQNLLGIINDLLDFSKIEAGKLELENVDM
                     DLRRTIEDVVDLLAERAHIKGLELACSIAAELTTQVKGDPLRLGQVLTNLVGNAIKFT
                     EHGAVVIRLVCTEETAQKVSMRFEVSDTGVGISQEAQSRIFDEFSQADGSTTRKHGGS
                     GLGLAISKQLVEMMGGSIQVDSTPGAGSTFSFTSSFEKQEAPSQEHVPAAPMGMLTGV
                     RALIVESGTVNRGILHAQMSNWGMTNRTAATPEQAIELLAEAATRGAPYDIAIIDLGL
                     PGMDAPELARAIRARAGIARVRLIMLTRRQGDIRNARDAGFDACLAKPVRQTVLYECL
                     VNVMAGQPQEAVAAPALPVPVSTPAAGARGSVLLVEDNLINQQVALGILQIQGYNVTV
                     VNNGREALEAHAQGAFDLILMDCHMPEMDGFEATREIRARERPSGERVAIVALTANAM
                     AQDREACLNAGMDDHLAKPFSMLTLQNMLDRWMPRSSAQPAAEPPAARAAVRGSAVLD
                     HEVLDELGKVRINGKPELLARVISLYIAESPKLMHKLKQAAGADDAPEIALCAHSLKS
                     SSANVGATALSRCYAEIEAAARRADTAAAGK"
     gene            complement(3033..3878)
                     /locus_tag="VGO02_00640"
     CDS             complement(3033..3878)
                     /locus_tag="VGO02_00640"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV7476224.1"
                     /translation="MIRRAIARAAAILGAAFALHSPGAAAQQAYAFNVLNQRTIALTA
                     QYWNPILTHVSKASGVPLELKLAKTAQEGNAVAEKGGYAFLYTNHFFTPERDRLGYKV
                     IARPAGPGIRSQIVVPQDSPIQTLQGLNGKDVAFVSRDGFTGYWLPLDALLRAKVNVN
                     VVFTGNQEASSAQLRINKVAAAGVNSSVMARYGRRESFEYRALWTSEIYQDLCIMANP
                     KVPADKVAAVRAAFINMAKDAEGRQVLEAGAELLKSNGELGFVAAENRDYDNYRKFYK
                     TTQVK"
     gene            complement(3898..4593)
                     /locus_tag="VGO02_00645"
     CDS             complement(3898..4593)
                     /locus_tag="VGO02_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV7476225.1"
                     /translation="MSAYFIRGITGFGSALISVPLLALSQPLQFAVPLVLALDFTASL
                     VLGGINRKQANWSEIRLLLPFGMIGACIGAYALVTLPTTPVLLTLGAFTMFFGFRNIF
                     GLQPVGQLSRAWAAPAGLAGGATGALFGAGSPPYIMYLTRRLLDKSAVRATFSWLIAI
                     DGGFRLTLFLLAGLLLDTKFQTAYALGLIPMGIGLYSGNKVHLDMTSEGMLRVVGALL
                     VLSGAMLFLKVAI"
     gene            complement(4776..5132)
                     /locus_tag="VGO02_00650"
     CDS             complement(4776..5132)
                     /locus_tag="VGO02_00650"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEV7476226.1"
                     /translation="MSERTPLLDPELIDEIRRVERATGRNDVFSGFVHKLEGFLAGFA
                     ADFAACVARGDAAAAVRAAHTLKGTCRQLGAPALGDLFADIERSAKAGDYVEAKRKFD
                     GAADLIAQSLEALKRA"
     gene            5202..>6036
                     /locus_tag="VGO02_00655"
     CDS             5202..>6036
                     /locus_tag="VGO02_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HEV7476227.1"
                     /translation="MTHRALLEQSFRAAVMAADPLKILAPHLPQPPRGRTFVAAAGKA
                     AASMAVAVENHWAANAPLDGIAVTRYQHGLPTRRIRVVEAGHPVPDEAGERSASEILR
                     RTRELGADDLLLALLSGGGSSLLALPAPGVSMAELKSLTGQLLRSGADIQEINTVRKH
                     LSAIQGGRLAAACRAPVLALVISDVTGDDPTHIASGPCAPDPTSCADAAAVLASYRID
                     FKGKFSETPKPGDPVFARVENRVVATAYRSLEAAAEVFRKQGITPVILGDTITGEARE
                     VA"
BASE COUNT          894 a         2052 c         2031 g         1059 t
ORIGIN      
        1 cttgccggct gcggcggtgt cggcgcggcg cgccgcggct tcgatctccg cgtagcagcg
       61 gctcagcgcc gtggcgccga cgtttgcgct gctcgatttg agggagtggg cgcacagcgc
      121 gatctcgggc gcatcgtccg cgcccgccgc ctgcttgagc ttgtgcatga gcttgggcga
      181 ctcggcgatg tacaggctga tgacgcgggc gagcaattcc ggcttgccgt tgatgcgcac
      241 cttgcccagc tcatcgagca cctcgtgatc gagcaccgcg gagcccctta ccgccgcccg
      301 cgctgcgggc ggctcggccg ccggctgggc ggacgagcgc ggcatccagc gatccagcat
      361 gttctgcagc gtgagcatgc tgaagggctt ggccaggtgg tcgtccatgc cggcgttgag
      421 gcatgcctcg cggtcctgcg ccatcgcatt ggcggtcagc gcgacgatcg cgacgcgctc
      481 gcccgagggc ctctcgcgtg cgcggatttc ccgcgtcgcc tcgaacccgt ccatctcggg
      541 catgtggcaa tccatcagga tcaggtcgaa ggcgccctgc gcgtgcgcct ccagcgcttc
      601 gcggccgttg ttgaccacgg tcacgttgta gccctggatc tgcaggatgc cgagcgccac
      661 ctgctggttg atgaggttgt cctccaccag caggacgctg ccgcgggccc cggccgcagg
      721 ggtgctcacg ggaaccggca gggcgggggc tgcgaccgcc tcctgcggct ggcctgccat
      781 gacgttgacc aggcattcgt acagcacggt ctggcgcacc ggcttggcga ggcaggcgtc
      841 gaagcccgcg tcgcgtgcgt tgcggatgtc cccctggcgg cgggtcagca tgatcagccg
      901 caccctggcg attccggcgc gcgcccggat ggcgcgcgcg agctccggcg cgtccatgcc
      961 cggcagcccc aggtcgatga tcgcaatgtc gtagggagcg ccgcgtgtcg ccgcctcggc
     1021 cagcagctcg atcgcctgct ccggcgtggc ggcggtccgg ttggtcattc cccagttgct
     1081 catctgcgcg tggaggatgc cgcggttgac ggtgcccgac tcgacgatca gcgcgcgcac
     1141 gccggtgagc atgcccatcg gcgccgcagg cacgtgttcc tgcgacggcg cctcctgctt
     1201 ctcgaagctg ctggtgaacg agaaggtgga gccggcgcca ggcgtggaat cgacctggat
     1261 gctgcccccc atcatctcga ccagctgctt gctgatggcg aggcccaggc ccgagccgcc
     1321 gtgcttgcgg gtagtcgatc cgtcggcctg cgagaactcg tcgaagatgc gcgactgcgc
     1381 ctcctggctg atgccgacgc cggtgtcgct gacctcgaag cgcatgctga ccttctgcgc
     1441 ggtctcctcg gtgcagacga ggcggatcac gaccgcgccg tgctcggtga acttgatggc
     1501 gttgcccacc aggttggtca gcacctggcc caggcgcagg ggatctccct tcacctgggt
     1561 ggtgagctcg gcggcgatgc tgcaggcgag ctccagcccc ttgatgtgcg cgcgctcggc
     1621 gagcagatcg acgacgtcct cgatggtgcg gcgcagatcc atgtcgacat tctcgagctc
     1681 gagcttgccg gcctcgatct tggagaagtc cagcagatcg ttgatgatgc cgagcagatt
     1741 ctggcccgag cgctgcaccg tctcggcgta cttgcgctgg ttgctggtca gcccggtttc
     1801 cagcagcagc tcggtcatgc ccagaacgcc gttcatcggc gtgcggatct catggctcat
     1861 cctggcgagg aacgagcttt tcgcgtggtt cgccgcttcc gccgcctcgc gcgccatgcg
     1921 cagcgcttcc tcgctgcgct tgtgctgcgt gacgtcgcgc gccaccgcga tgctgccgat
     1981 gcactggttg gcatcgtcca gcaggggcgc gaccgcgacc tggatgtcga tgggcgtgcc
     2041 cgagcgcgtc gtggccttgg cgtcgtagga aaatttgtcg ccggtcatca gccgctgcat
     2101 gcgcgccgct tcctcctgcg gcgtggactc ggccacgttc agcgcacgcc cgatcgcctc
     2161 ggtcggcgta tagccgaaca tggcggcggc gccggagttc cagctggtga tgttgccggc
     2221 gaggtccttg gtccagatcg cttcgctcga ctgctcgacg atggtcgcca gcagcttgtt
     2281 cttcgattcg ctcttgccga gcgaggcaat caggccctcg atgctgttcg ccatgttgtt
     2341 gaatgcttcg gcagccacgc ttacctcggg cgcgccttca ggctcgatgc gcacggcgtg
     2401 gtcgccctgg ctgaaacggt tggcgccctc ggccagcatg cgcagcgtgc cgaggttgcc
     2461 gcggaagatg agccagatga actgcagcat gagaaacagg gtcacggcga cgatctgcag
     2521 ctgcttgacg aactgcgccc agagccggtt ctgcgccctg accggggtca tgctggcgct
     2581 gagcgtgccg tatcccaccc cgccggccgt gacgtcgatg ctctggtcct ggtgctcgat
     2641 cgcgacaagg cgggcgaacc aggtcggcgc gtccagcttg tcgggcgcgt cctgggcgac
     2701 cagcgtcttg ccgtcgcggt cggtccaggc gaagcggtcg acctcgccct tcttcacctg
     2761 gttcttcagc agctggctga tcgcctcgta ctcgccgacc agcgcctggt cggcaaccag
     2821 cggcgcgagg aaggccagcg tttccttctg ctcgcgcagc agccgggcgg agtgctcgcc
     2881 cgcgccctcg gccacgagca gggcggtctg cacgccgcct ccggtgatga tcgcgattcc
     2941 cacggcaagc atgaggcgcg aaaccagccc ccagtctttc cagaatcgca tccggcgcgg
     3001 gttgttcatc gtcgctcctt gtgcctgacg acctatttga cctgcgtggt cttgtagaac
     3061 ttgcggtagt tgtcgtagtc gcggttctcg gccgcgacga agcccagctc gccgttgctt
     3121 ttcagcagct cggcgccggc ctcgagcacc tggcggccct ccgcgtcctt ggccatgttg
     3181 atgaaggcgg ccctgaccgc ggccaccttg tcggcgggca ccttggggtt ggccatgatg
     3241 cacaggtcct ggtagatctc ggaagtccac agcgcgcggt actcgaacga ttcgcggcgg
     3301 ccgtagcgcg ccatgaccga gctgttgacg ccggcggccg ccaccttgtt gatgcgcagc
     3361 tgggcggacg aggcttcctg gtttcccgtg aacaccacgt tgacgttcac cttggccctc
     3421 agcagcgcgt ccagcggcag ccagtagccg gtgaagccgt cccgcgacac gaaagcgacg
     3481 tccttgccgt tcaggccctg cagcgtctgg atcggcgaat cctgcggcac cacgatctgc
     3541 gagcggatac cgggcccggc cgggcgcgca atcaccttgt agccgaggcg gtcgcgctcc
     3601 ggggtgaaaa agtggttggt gtagagaaag gcatagccgc ccttctcggc gacggcgttg
     3661 ccttcctgcg ccgtcttggc cagcttcagc tcgagcggca ccccgcttgc cttgctgacg
     3721 tgcgtaagga tcggattcca gtactgcgcg gtcagcgcga tggtgcgctg gttgaggacg
     3781 ttgaaggcgt acgcctgctg cgcggcggcg cctggcgagt gcaaggcgaa cgctgcgccc
     3841 aggatcgctg cggcacgcgc gattgctctt cggatcatga ccgtctcccc cggcatttca
     3901 aatggccacc ttcaggaaca gcattgcgcc gctgagcacc agcagcgcgc cgaccaccct
     3961 cagcatgcct tcgctggtca tgtccaggtg gaccttgttg ccgctgtaca gcccgatccc
     4021 catggggatg agacccagcg cataggcggt ctggaacttc gtatcgagca gcaggccggc
     4081 cagcaggaac agcgtgagcc ggaagccgcc gtcgatggcg atcagccacg agaacgtggc
     4141 gcgcaccgcg cttttgtcga gcaggcggcg cgtcaggtac atgatgtagg gtggcgagcc
     4201 cgcgccgaac agcgcgccgg tcgcgccgcc ggccagcccc gccggcgcgg cccaggcccg
     4261 cgacagctgc cccaccggct gcaggccgaa gatgttgcga aagccgaaga acatcgtgaa
     4321 ggcgcccagc gtcagcagca ccggcgtggt gggcagcgtg accagggcgt aggcgccgat
     4381 gcacgcgccg atcatgccga acggcagcaa tagcctgatc tcgctccagt tcgcctgctt
     4441 gcggttgatg ccgccgagaa ccagcgaggc ggtgaagtcg agcgcgagga ccagcggtac
     4501 tgcaaactgc agcggctgag acagcgcgag cagcggcacg gagatcagcg cggagccgaa
     4561 cccggttatg ccgcgtatga aatacgcgct catcaggatg cacaacacca ataccatgtc
     4621 agccactgcc ggtttccctt gttcctttcc cttgtcctcc ttgaattaag aggatttcgc
     4681 tattgttacc cggggggcgg gcggcattgg ccgtgtttac cttgttttgc aatcaggccc
     4741 gctatgtatc tgtgttttat cgttaattcg ccctctcagg cgcgcttcag cgcctccagc
     4801 gactgggcga tcaggtctgc cgcgccatcg aacttgcgct tcgcttccac gtagtcgccg
     4861 gccttcgcgc tgcgctcgat atcggcgaac agatcgccca gcgccggcgc gccgagctgg
     4921 cggcaggtcc ccttcagcgt atgcgcggcg cgcacggcgg cggcggcgtc gccgcgcgcc
     4981 acgcaagccg cgaaatccgc cgcgaagccc gccagaaaac cctcgagctt gtggacaaac
     5041 ccggaaaaca cgtcgttcct tccggtggcg cgctcgacgc gcctgatctc atcgatcaac
     5101 tccggatcga gcaacggggt gcgctcgctc attgttcctg tcccaaaggt gatgcacgat
     5161 aacctagata attgcatccc gcttccgacc gtgcacagcg catgacccac agagcactgc
     5221 tcgagcagag cttccgcgcc gcggtcatgg ccgccgatcc gctgaagatt ctcgcgcccc
     5281 atctgccgca gccgccgcgc gggcgcacgt tcgtcgcggc cgccggcaag gccgcggcct
     5341 ccatggccgt ggcggtggag aaccactggg ccgcgaacgc gccgctcgat ggaattgcgg
     5401 tcacgcgcta ccagcacggc ctgcccaccc gacgcatccg ggttgtcgag gcggggcacc
     5461 cggtcccgga cgaagccggg gagcgctcgg caagcgagat cctgcgcagg acgcgcgagc
     5521 tgggtgcgga cgatctgctg ctggcgctgc tctcgggcgg cggctcgagc ctgctggccc
     5581 tgccggcgcc cggcgtatcg atggccgagc tcaagtccct gaccgggcag ctcctgagga
     5641 gcggcgccga catccaggaa atcaacacgg tgcgcaagca cctttccgcg atccagggcg
     5701 gaaggctggc ggcggcgtgc cgcgcgccgg tgctggcgct tgtcatctcg gacgtcacgg
     5761 gagacgaccc gacgcacatc gcctcggggc cctgcgcccc ggacccgacc agctgcgccg
     5821 acgcggcggc cgtgctcgcg agctaccgca tcgacttcaa ggggaaattc tcggagacgc
     5881 ccaagccggg cgacccggtc tttgcccgcg tcgagaaccg cgtcgtcgcc accgcatacc
     5941 gctcgctcga ggccgccgcg gaggtcttcc gcaagcaagg catcacgccg gtgatcctcg
     6001 gcgacacgat caccggtgag gcgcgggaag tggcgc
//
LOCUS       DASYJA010000031         4402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_459828, whole genome shotgun sequence.
ACCESSION   DASYJA010000031 DASYJA010000000
VERSION     DASYJA010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459828"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VGO02_00660"
     CDS             <1..536
                     /locus_tag="VGO02_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7476228.1"
                     /translation="NHGGRVEARSAGPGRGAEFIIHLPLAASSAAAPAPPVPLPANGA
                     RRRVLVVDDNADAAESIARLLRLEGHRVETALNGVAALRAAEALQPDIAFIDLNMPGM
                     DGYELTRRLRALPCGRNARLVALTGMGKASDVDHTRAAGFDLHLTKPADPAQVSQLAA
                     GAAPDDNVVAFRDSEAG"
     gene            complement(538..1737)
                     /locus_tag="VGO02_00665"
     CDS             complement(538..1737)
                     /locus_tag="VGO02_00665"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7476229.1"
                     /translation="MGRSLPVWLLIALSGTIISLCMGMRQSLGLFMRPMGIDLGITAA
                     AFGFSIALQNIVWGLAQPFSGAAADRYGPRPVLIVTSLVFAAGLLLFMVSNSFPGALV
                     LAGLLLGFGTTGFSNLYGTISRATPLEKRSQTVGLVAATGSVGTFVLAPLAGSLIDGP
                     GWRTAMVVFVALAASMALLSLPIREIAEKNTVERKMGLGEALREATGHRGYLFMTLAF
                     FACGFQLVFLTTHLPAYLALCGVAPGVGATALGLIGLFNAIGTYIFGQLGARYSQKHL
                     LALIYLLRTLFIAAFLLVPISAASTLVFASAMGFLWLGVAPLVTGIIARVFGLTHFNT
                     LYGVVFLSHQVGSFFGAWMGGLVFDWSGNYNFAWGALIVIGLAAFTLQWLMDESPPRE
                     RAAMMPA"
     gene            complement(1787..1969)
                     /locus_tag="VGO02_00670"
     CDS             complement(1787..1969)
                     /locus_tag="VGO02_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476230.1"
                     /translation="MVSKATKLAAKKRLAQTKVQLAKFKVRARKEMARMSVVLKRHQR
                     ALKAAAKRYKSSLKQA"
     gene            complement(1987..3093)
                     /locus_tag="VGO02_00675"
     CDS             complement(1987..3093)
                     /locus_tag="VGO02_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEV7476231.1"
                     /translation="MDVRAAVAHKAGAPLVIETVRLDGPKAGEVLVEIKATGLCHTDE
                     FTRSGADPEGLFPAILGHEGAGIVVDVGPGVTTLRKGDHVIPLYTPECRQCKSCLSRK
                     TNLCTAIRATQGKGLMPDGTSRFSLNGKPVHHYMGCSTFANFTVLPEIALAKIREDAP
                     FDKVCYIGCGVTTGIGAVINTAKVEPGASVVVFGLGGIGLNVVQGARMAGANMIVGVD
                     LNPKREALARKFGMTHFVNPKDVKDLVPYLVDLTGGGADYSFECIGNVDVMRQALECC
                     HRGWGVSVIIGVAGAGQEIRTRPFQLVTGRVWKGTAFGGARGRSDVPRIVDWYMDGRI
                     NIDDLITHELPLERINDGFDLMHNGESIRSVVVY"
     gene            3116..3889
                     /locus_tag="VGO02_00680"
     CDS             3116..3889
                     /locus_tag="VGO02_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476232.1"
                     /translation="MLFFIAALLAACGAHAQPDGVNVTALRDPVDKSYRRMVAGMDYF
                     EKRHALAPAAALRFKLLPRNRGTRMEDVEVAIVADSFEVPVAVAPDLTFTLERNAQAL
                     KENASVRPNRKKQTLTWRAEIRTPGLAPGTRRLGDLRLECEVGLVARLISNYRPSMIG
                     YLSSLFAEGPAYCERPEPRYLFFADRPIWAVTLVSGARRETLPVDRLYAGASRDPDWK
                     ADLKYCDCEVLADRTYFMPLDDRSWPDDTRVEFEYMDDR"
     gene            3886..4185
                     /locus_tag="VGO02_00685"
     CDS             3886..4185
                     /locus_tag="VGO02_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476233.1"
                     /translation="MKLVFLLLILAGCATPTRTPPPGPGEVASAQAARDAVILGKSTR
                     ADVRAILGKGTEIPFDTGYEVWVYRQQLKEKEKPPRSELVLLFDRAGILSKARLR"
     gene            4221..>4402
                     /locus_tag="VGO02_00690"
     CDS             4221..>4402
                     /locus_tag="VGO02_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEV7476234.1"
                     /translation="MPKAYWVVQYKSIKNPEKLAAYAKIAGPAIQAAGGKFLVRSPSP
                     AKFYELGMNQRTVVTE"
BASE COUNT          753 a         1566 c         1412 g          671 t
ORIGIN      
        1 agaaccacgg cgggcgcgtc gaagcgcgca gcgccggacc cggccgcggc gccgagttca
       61 tcatccacct gccgctcgcg gcctcgtccg ccgccgcgcc cgcgccgccc gtcccgctgc
      121 ccgcgaacgg cgcgcgccgg cgcgtgctgg tggtcgacga caacgccgac gcggcggaga
      181 gcatcgccag gctgctgcgc ctcgagggcc accgcgtcga gacggcgctc aacggtgtcg
      241 cggcgctgcg cgccgccgag gccctgcagc ccgatatcgc tttcatcgac ctcaacatgc
      301 ccggcatgga cggctatgag ctcacgcggc gcctgcgcgc gctgccctgc gggcgcaacg
      361 cacgcctcgt cgccctgacc ggcatgggca aggcgagcga cgtggatcac acccgcgcgg
      421 cgggcttcga cctgcacctc accaagcccg ccgaccccgc gcaggtctcg cagctcgccg
      481 ccggcgcggc cccggacgac aacgtcgtcg cgttccgcga cagcgaagcc ggatagatca
      541 ggcgggcatc atcgccgcac gctcgcgtgg cgggctctcg tccatcagcc actgcagcgt
      601 gaaggcggcg aggccgatga cgatgagcgc gccccaggcg aagttgtagt tgcccgacca
      661 gtcgaagacc aggccgccca tccaggcgcc gaagaacgag ccgacctggt ggctgaggaa
      721 gaccacgccg tagagcgtgt tgaagtgcgt caggccgaag acgcgggcga tgatgccggt
      781 gaccagcggc gccacgccca gccagaggaa gcccatcgcg gaggcgaaca ccagcgtcga
      841 ggcggcgctg atcggcacca gcaggaaagc cgcgatgaaa agcgtgcgca gcaggtagat
      901 cagcgcgagc aggtgcttct ggctgtagcg cgcgccgagc tggccgaaga tgtaggtgcc
      961 gatggcgttg aacaggccga tcaggccgag cgcggtggcg ccgacgccgg gcgccacgcc
     1021 gcacagcgcg aggtacgccg gcaaatgtgt cgtcaggaac accaactgga agccgcaggc
     1081 gaagaaggcg agcgtcatga agaggtagcc gcggtgcccg gtggcctcgc gcaaggcctc
     1141 gcccagcccc atttttcttt caaccgtatt cttttccgct atttccctga tcggcagcga
     1201 cagcaacgcc atcgacgccg ccagcgcaac gaacaccacc atcgccgtgc gccagcccgg
     1261 accgtcgatc agcgagcctg cgagcggcgc gagcacgaag gtgccgaccg agcccgtggc
     1321 ggccaccagg cccaccgtct ggctgcgctt ctcgagcggc gtggcgcgcg agatggtgcc
     1381 gtagaggttg ctgaagccgg tggtgccgaa gcccagcagc aagccggcga gcaccagggc
     1441 gcccgggaag ctgttcgaca ccatgaaaag cagcagaccg gctgcgaaga cgagcgaggt
     1501 gacgatcagc accgggcgcg ggccgtagcg gtcggcggcc gcgcccgaga agggctgcgc
     1561 cagtccccag acgatgttct gcagcgcgat cgagaaaccg aaggccgccg ccgtgatgcc
     1621 gaggtcgatg cccatcggcc gcatgaacaa gcccaggctc tgccgcatgc ccatgcacag
     1681 cgagatgatc gtgccgctga gcgcgatcag cagccagacc ggcaggcttc ggcccatcat
     1741 ccggcgacgc taccccgccg gcgggccgat tctgtcattt gtaatactag gcctgcttga
     1801 gcgagctctt gtagcgcttc gcggccgcct tcagcgcgcg ctggtgtctt ttcaggacca
     1861 ccgacatcct cgccatctcc ttcctcgccc tgaccttgaa cttggcgagc tggaccttcg
     1921 tctgggcgag cctcttcttc gcggcgagct tcgttgcttt gctgaccatc ggcagtctcc
     1981 cttccttcag tagacgacga cactccggat cgactcgcca ttgtgcatca ggtcgaagcc
     2041 gtcgttgatg cgttccagag gcagctcgtg ggtgatgaga tcgtcgatgt tgatcctgcc
     2101 gtccatgtac cagtcgacga tcctcggcac gtcgctgcgc ccccgcgcgc cgccgaaggc
     2161 ggtgcctttc cacacgcggc cggtgaccag ctggaacggg cgcgtcctga tctcctgccc
     2221 cgcgccggcc acgccgatga ttactgacac gccccagccg cggtggcagc attccagggc
     2281 ctggcgcatc acatcgacat tgccgatgca ctcaaaactg taatcagctc cgccaccggt
     2341 caggtcgacc aggtatggaa caaggtcctt gacgtctttt gggttgacga aatgcgtcat
     2401 gccgaattta cgcgccaagg cctcgcgttt gggattcaaa tcgacaccga cgatcatgtt
     2461 cgcgcccgcc atgcgcgcgc cctgcaccac gttgaggccg atgccgccga ggccgaagac
     2521 caccacgctg gcgcccggct cgaccttcgc cgtgttgatc accgcgccga tgccggtggt
     2581 cacgccgcag ccgatgtagc agaccttgtc gaacggcgcg tcctcgcgga tcttcgccag
     2641 cgcgatctcg ggcagcaccg tgaagttggc gaaggtggag cagcccatat agtgatggac
     2701 gggttttcca ttcaatgaaa atctggacgt gccatcgggc atcaggccct tgccctgcgt
     2761 tgcgcgaatc gccgtgcaca ggttggtctt gcgcgacagg cacgacttgc actgccggca
     2821 ctcgggcgta tagagcggaa tcacgtggtc gcccttcctg agcgtcgtca cacccgggcc
     2881 gacgtcgacc acgatgccgg cgccctcgtg gccgaggatc gccgggaaca ggccttccgg
     2941 gtcggcgccc gagcgcgtga attcatccgt atgacagagg ccggtcgcct tgatctcgac
     3001 cagcacctcg cccgccttcg gcccgtcgag ccgcaccgtc tcgatcacga ggggcgcacc
     3061 cgccttgtgc gccacggcag cacggacgtc catgccttaa tatagccgac acaaggtgct
     3121 tttcttcatt gcggccctcc tcgcggcctg cggcgctcat gcgcagccgg acggcgtgaa
     3181 tgtcaccgcc ctgcgcgatc cggtcgacaa gtcctaccgc cggatggtcg ccggcatgga
     3241 ttacttcgag aagaggcacg ccctcgcgcc cgcggccgcg ttgcgcttca agctcctgcc
     3301 gcgcaaccgc ggcacccgca tggaagacgt cgaggtcgcg atcgtcgcgg acagcttcga
     3361 ggtcccggtc gcggtggcgc cggacctcac cttcaccctc gagcgcaacg cgcaggcgct
     3421 gaaggaaaac gcctcggtgc ggccgaaccg caagaagcag acgctgacct ggcgcgccga
     3481 gatccgcacc cccggtcttg cgccgggcac gcggcgcctc ggcgacctgc gcctggaatg
     3541 cgaggtcggg ctggtggcgc gcctgatttc gaactaccgg ccctccatga tcggctacct
     3601 ctcgagcctg ttcgccgaag gcccggcgta ctgcgagcgc cccgagccgc gctacctgtt
     3661 cttcgccgac cggccgatct gggccgtcac cctggtctcc ggcgcgcgcc gggaaacgct
     3721 ccccgtcgac cgcctgtacg ccggcgcgag ccgcgacccg gactggaaag cggacctcaa
     3781 gtactgcgac tgcgaggttc tcgccgaccg cacctacttc atgccgctcg acgaccgcag
     3841 ctggcccgac gacacccgcg tcgagttcga gtacatggac gaccggtgaa gctcgtcttc
     3901 ctcctgctga tcctcgccgg gtgcgccacg ccgacccgca cgccgccgcc gggtccgggc
     3961 gaggtcgcct ccgcccaggc cgcgcgcgac gcagtcatcc tcggcaagag caccagggcg
     4021 gacgtgcgcg ccatcctcgg caagggcacc gagatcccct tcgacaccgg ctacgaagtc
     4081 tgggtgtacc gccagcagct gaaggagaag gagaagcctc cccgcagcga gctggtgctg
     4141 ctcttcgacc gtgccggtat cctctccaag gcgcgcctgc gctagcattc cttccacctt
     4201 cacaatcagg agacaacacc gtgccgaaag cctattgggt cgtgcaatac aagtcgatca
     4261 agaatcccga gaagctcgct gcctacgcaa aaatcgccgg ccccgccatc caggcggcag
     4321 gcggcaagtt cctggtgcgc tcgccctcgc ccgcgaagtt ctacgagctg ggcatgaacc
     4381 agcgcaccgt ggtcacggag tt
//
LOCUS       DASYJA010000032         2317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_641543, whole genome shotgun sequence.
ACCESSION   DASYJA010000032 DASYJA010000000
VERSION     DASYJA010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641543"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VGO02_00695"
     CDS             <1..693
                     /locus_tag="VGO02_00695"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7476235.1"
                     /translation="LATSYDRLRMRAGRRAALSDSQQARLRRATISRRVTARAGANGS
                     AFHPRREELLAAAAKVFSARGYGGASLREICAAAGILAGSMYHHFRSKEDLFVSVHAE
                     GFRHLNEVVDRALEGKADPWGRLEAAIGAHLGEVVERTDLVAVTSASLFHMEGSRLQR
                     RLNRERTAYEDRFRKLILVLPLPADVDPSLLRLTLLGAINWTRVWYRPGKRSPQQIAH
                     HLVHKVLRKSLG"
     gene            705..1676
                     /locus_tag="VGO02_00700"
     CDS             705..1676
                     /locus_tag="VGO02_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HEV7476236.1"
                     /translation="MQTRRKFLGAAALGLFALPIATRAQGFETAKILVGFPPGGTTDV
                     MARKVADKLRGPYARGVIVENRPGAGGQLGVVALKDAPADGSTMLLTPSSMLTIYPNT
                     YPKLQYRLEDVAPVSLAMYSHHVLSVGPAVPDSVKTLREFLAWTKVNPDKAVYGSPGA
                     GSMPHLIGVLLAKSVGADLRHVAYRGSAPGIQDLLGGQIASFLGPTGDVLAHSQAGKV
                     RVLGTSGKERSAFLPAVPTMGEQGFKIAVREWYAFFMPARASADTVQRAAAALQGVLG
                     QAEVVEFAKQFGLEVQGSSPRQLADVLKVDTAEWGGLVKETGFTAES"
     gene            1673..>2317
                     /locus_tag="VGO02_00705"
     CDS             1673..>2317
                     /locus_tag="VGO02_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7476237.1"
                     /translation="MSKPLDKRRTRSVRLASQAVVAEHRAGNVYLRSPHPLAEYPRRI
                     TERLDHWAEHAPERVFLAQRGPDGAWLHLTYAQARSRARRIAQALIERRLSAERPVSV
                     LSGNDIEHALIQLGALYAGVPYAPVSPAYSLLSKDFAKLKHVMRVLTPGLVFASQREP
                     FKSAIAAAVPGSIEIIHGDEFHMLEAKAPTSAVDEAHARVGPDTIAKFLFTSGST"
BASE COUNT          357 a          783 c          807 g          370 t
ORIGIN      
        1 ctggcgacca gctatgaccg tctcaggatg cgcgccggcc gccgcgcggc gctctccgat
       61 tcccagcagg cgcggctgcg ccgcgcgacc atcagccggc gggtaaccgc gcgtgccgga
      121 gcgaacggct ccgcgttcca cccgcggcgc gaggagctgc tcgccgccgc cgccaaggtt
      181 ttcagcgcaa gggggtacgg aggggcatcg ctgcgcgaga tctgcgctgc cgccggcatc
      241 ctcgcggggt cgatgtacca ccacttccgc tcgaaggagg atctgttcgt cagcgtgcac
      301 gcggaaggct ttcgccacct gaacgaagtc gtggatcggg cgctcgaggg caaggccgat
      361 ccttggggac ggctcgaggc ggccattggt gcgcacctgg gcgaggtggt agagcggacc
      421 gatctggtcg cggttacctc cgccagcctg ttccatatgg aaggcagccg gctgcagcgc
      481 cgcctcaacc gcgagcgcac ggcgtatgaa gaccgcttcc gcaagctgat cctggtgctg
      541 ccgctacctg cggacgtcga ccccagcctg ctgcgcctga cgctcctggg tgcgatcaac
      601 tggacgcgcg tctggtaccg gccgggcaag cgcagcccgc agcagatcgc gcatcatctg
      661 gtgcacaagg tgttgcgcaa gagcctcggc tgacaggagg tagcatgcag acacgtcgta
      721 aattcctcgg cgctgcggcg cttggtctgt tcgcgttgcc gattgccact cgcgcgcagg
      781 gcttcgagac ggcgaagatc ctcgtcggtt ttccgccggg cggcaccacc gacgtcatgg
      841 cgcgcaaggt cgccgacaag ctgcgcggcc cctacgcgcg cggcgtgatc gtggagaacc
      901 gtccgggggc cggcggacag ctcggcgttg tagcgctgaa ggacgcaccg gcggatggca
      961 gcacgatgct gcttacgccg tcgtcgatgc tcacgatcta cccgaatacc tatccgaagc
     1021 tgcagtacag gctcgaggac gtggcgccgg tgtcgcttgc catgtacagc caccacgtgc
     1081 tttccgtcgg tcctgcggtg ccggacagcg tgaagacgct gcgggagttc ctcgcctgga
     1141 ccaaggtcaa tccggacaag gcggtctacg gcagcccggg cgcaggctcg atgccgcacc
     1201 tcatcggcgt gctgctcgcc aagtcggtcg gcgcggatct ccggcacgtc gcgtaccgcg
     1261 gaagcgcgcc ggggatccag gacctcctcg gcgggcagat cgcgtctttc ctcgggccga
     1321 ctggcgacgt gctcgcgcat tcgcaggccg ggaaggtgcg cgtgctcggc acctccggca
     1381 aggagcgcag cgccttcctg cctgccgtgc cgaccatggg cgagcagggc ttcaagatcg
     1441 cggtgcgcga gtggtacgcg ttcttcatgc cggcacgcgc gagcgcggac accgtgcagc
     1501 gcgccgccgc ggcgctgcag ggggtgctcg gccaggccga ggtggtggag ttcgcgaagc
     1561 agttcggcct ggaggtgcag ggctcgtccc cgcgccagct cgccgacgtg ctcaaggtgg
     1621 acacggccga gtggggcggc ctggtcaagg agaccggctt caccgccgaa tcttgagcaa
     1681 gccgctggac aagcgcagga cgcgaagcgt tcgactggcc agccaggcgg tcgtagccga
     1741 gcatcgcgcc ggcaacgttt atctgcgctc accgcacccg ctcgccgagt atccccgcag
     1801 gatcaccgag cggctcgacc attgggccga gcacgcgccg gagcgcgtgt tcctcgcgca
     1861 acgcgggccg gacggcgcgt ggctgcacct cacctatgcg caggcgcgca gccgcgcgcg
     1921 gcgcatcgcg caggcgctga tcgagcggcg gctttccgcc gagcgaccgg tgtcggtcct
     1981 ctccggcaac gatatcgagc acgcgctgat ccagcttggc gcgctttacg caggcgtgcc
     2041 gtatgcgccg gtatcgccgg cgtattccct gctttcgaag gatttcgcca agctgaagca
     2101 cgtcatgcgc gtgctgaccc cggggctcgt gtttgcttcg caaagagagc ctttcaagtc
     2161 ggccattgca gccgccgtgc ccggcagcat cgagatcatc catggagatg agttccacat
     2221 gctcgaggcg aaggcgccca ccagcgcggt ggacgaggcg catgccaggg tgggcccgga
     2281 cacgatcgcg aaattcctct ttacctccgg ctctacc
//
LOCUS       DASYJA010000033         5695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2366272, whole genome shotgun sequence.
ACCESSION   DASYJA010000033 DASYJA010000000
VERSION     DASYJA010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2366272"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VGO02_00710"
     CDS             complement(<1..502)
                     /locus_tag="VGO02_00710"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEV7476238.1"
                     /translation="MGESYGAWKMGADAEVMPFITSANIACGFHGGDPATIRKTVRLA
                     VDHGVAIGAHPSLPDIQGFGRRVMKISPQDMYDLVVYQVGAVEAFARAAGGKLHHIKC
                     HGALYNMAATDEGLSDAMARAAKDLGNIMVYALSNSTMMKVVKQHNLPVAGEVFADRG
                     YSDDGTL"
     gene            complement(526..1494)
                     /locus_tag="VGO02_00715"
     CDS             complement(526..1494)
                     /locus_tag="VGO02_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEV7476239.1"
                     /translation="MSVRVLRPGLLTTVQDRGRHGHQHLGLSPGGAMDAVALALANAL
                     VGNPPGVAALEITVIGPELLFEEDTLVALCGAEFLGGFPHNRPVLARAGSKLSVGRAV
                     RGARAYLAVAGGFWIEPVLGSCSTYLPGAFGGLDGRALRHGDVLPLAENCSKERFATL
                     KRKSGATVRWSAPPLTLPDREPIVVHAIEGQHFASFDATAQRAFFDTVWRVAPESNRM
                     GFRLDGAPLVRADSEILSGPTCLGSVQVPPSGAPIALMADHQTTGGYARIAEIVSADV
                     PRVAQLAPGATLHFALCSLQTARELRLDAKLRLDAALRSIAWEYGA"
     gene            complement(1491..2195)
                     /gene="pxpB"
                     /locus_tag="VGO02_00720"
     CDS             complement(1491..2195)
                     /gene="pxpB"
                     /locus_tag="VGO02_00720"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEV7476240.1"
                     /translation="MARLPKPLGVVPLGDLAAYVEFSRTLDLEVNSVAQRLAAAIVAR
                     ADPRIRDVVPALGGVALHFDPICEDDVLKAATELVEQCMKKGLPDADDLGRGVEVPIC
                     YDAEFALDLADLAQRLKISPEEIIKRHADAEYRVLMVGFAPGHPYIGGLDPKLAVPRR
                     SEPRVVVPAGSVAIANQQTVVYPYSMPGGWSVIGRTPLTSFDAGRPQPSLFSAGDRVR
                     FRPIDKRDFLSLRERQ"
     gene            complement(2196..2786)
                     /gene="nthA"
                     /locus_tag="VGO02_00725"
     CDS             complement(2196..2786)
                     /gene="nthA"
                     /locus_tag="VGO02_00725"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEV7476241.1"
                     /translation="MKDDQGKYQPLSYFQLMEVTLRELLVEKGIITEQQVDREVEEMR
                     ERTPERGAQVVARAWVDAAFKERLLLNGTKACEELGLEIPALKLVVVENTPQVHNAIV
                     CTLCSCYPRVLLGIPPDWYKSRNYRSRMVCEPRIVLEEFGLKIPEHVTIRVHDSTADM
                     RYLVLPMRPASTEGWSEERLAALVNRDSMIGVALPK"
     gene            complement(2783..3070)
                     /locus_tag="VGO02_00730"
     CDS             complement(2783..3070)
                     /locus_tag="VGO02_00730"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HEV7476242.1"
                     /translation="MASARFKPGEKVRVMKAYPLGHVRTPYYIRGCVGEVERICGAFA
                     NPEELAQMRSGLPPLPLYRVRFRQKDVWPDYRGGDADVVEVEIFEHWLDRA"
     gene            complement(3057..3380)
                     /locus_tag="VGO02_00735"
     CDS             complement(3057..3380)
                     /locus_tag="VGO02_00735"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476243.1"
                     /translation="MIERSHHDMGGLPAGKVEPTEHDYELWERRVDAMSVLLQGIKPG
                     TKRLITVDERRRAIEMLPPKAYDDMTYYERWVIALAETLIQRGILTTEEMARKLVEVE
                     SRGER"
     gene            complement(3377..4321)
                     /locus_tag="VGO02_00740"
     CDS             complement(3377..4321)
                     /locus_tag="VGO02_00740"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEV7476244.1"
                     /translation="MGRLKTHRLDPTPETVHWGYFDARLPARIEVESGDTVVVDTVSG
                     WRDVAPDESLYRPAHREIITKLKPELGAHILTGPVAVRGAQPGDTLEVRIKAIELTVD
                     WGWNVIRPLRGTLPEDFPAFERRTLPIDRKNMTATLPWGPTIPLQPFFGILATAPRPE
                     YGRISSVEPREFGGNVDCKEYVAGTSLFLPVFVPGANFSCGDGHAVQGDGEVCLSALE
                     TCLKGTFELVVHKKTNLAMPRAITPTHYITLGLDTDLDDAAKQALRDMIVWLVEMKGW
                     TRSEAYVFCSLACDLHVTQLVDGNKGIHAMVRRDSIRA"
     gene            complement(4309..5616)
                     /locus_tag="VGO02_00745"
     CDS             complement(4309..5616)
                     /locus_tag="VGO02_00745"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEV7476245.1"
                     /translation="MRPLTKWNAVLDRSADIPRVFRKAFEEMTTGRPGAAHIALPFDV
                     QNGPVDRSEVWADPTLGSYPSRRTTCDPLFVELAAKLLRTAKNPIFICGGGVVLSGAE
                     AELAELAEKICAPVATTISGKGSIDERCALSIGVVGSNGGTPETRAVVDAADLVIFVG
                     CRAGSVTTERWRHPAPGRTKIIHIDVDPAVPGTNYKVDVPLIGDAKLVLAALNEAVSA
                     APRELSFVNQKKDEKFRKFETLAASEDSPIKPERVVAELQKVLGKDSIIVADPGTPCP
                     YLSAYYLVRGTGRRYFSNRAHGALGYALAASIGAHFARPKVKTVAVMGDGSFGMCVGE
                     LETAVRLKLPITFVVISNAVYGWIKAGQKTGYGERYFSVDFGVTDHAKVAEAFGVKAF
                     RVFQPENLKQVLKEALSHGGPSLVDIVCQPLQEARAPVSEWVA"
BASE COUNT          918 a         1959 c         1876 g          942 t
ORIGIN      
        1 cgagcgtacc gtcgtcggag tagccgcggt cggcgaacac ttcaccggcg acgggaaggt
       61 tgtgctgctt cacgactttc atcatggtgg aattcgaaag cgcatacacc atgatgtttc
      121 ccagatcctt cgccgcgcgg gccatcgcgt cggacagccc ctcgtcggtg gcggccatgt
      181 tgtacagcgc gccgtggcat ttgatgtgat gcagcttgcc gccggcggcg cgcgcgaacg
      241 cttccaccgc gccgacctgg tagaccacca ggtcgtacat gtcctgcggc gagatcttca
      301 tgacgcgccg gccgaagccc tggatgtcgg gcagcgaagg atgcgcgccg atggccacgc
      361 cgtggtcgac ggcgaggcgc accgtcttgc ggatggtggc cgggtcgcca ccgtggaagc
      421 cgcaggcgat gttggcagag gtgatgaacg gcatgacttc ggcgtcggcg cccatcttcc
      481 aggcgccgta gctctcgccc atgtcgcagt tgaggtcgat tctcttcaag ctccgtactc
      541 ccatgcgatc gagcgcagcg ccgcatccag ccgcagcttg gcgtcgagcc gcagctcgcg
      601 cgccgtctgc aggctgcaca gcgcgaaatg cagcgtcgct cccggcgcga gctgcgcgac
      661 gcgcggcacg tcggcgctca cgatctcggc gatgcgcgca tagccgccgg tggtctggtg
      721 atcggccatc agcgcgatcg gcgcgccgct cggcggcacc tgcaccgagc cgaggcaggt
      781 cggcccggat agtatctcac tgtccgcgcg caccagcggc gccccgtcga ggcggaatcc
      841 catccggttc gactcgggcg ccacgcgcca gacggtgtcg aaaaaggcgc gctgcgccgt
      901 ggcgtcgaag ctcgcgaagt gctggccttc gatggcgtgc accacgatcg gctcgcggtc
      961 gggcagcgtc agcggcggcg ccgaccagcg cacggtcgcg ccgctcttgc gcttgagggt
     1021 ggcgaacctc tccttcgagc agttctccgc cagcggcagc acatcgccgt ggcgcagggc
     1081 gcggccgtcg agtccgccga aggcgccggg caggtaggtg ctgcagctgc cgagcaccgg
     1141 ctcgatccag aacccgcccg ccaccgcgag gtaggcgcgc gccccgcgca ccgccctgcc
     1201 gacggagagc ttgctgccgg cgcgcgccag caccggccgg ttgtgcggga agccgcccag
     1261 gaactcggcg ccgcacagcg cgaccagcgt gtcctcctcg aacagcagct ccggtccgat
     1321 caccgtgatc tcgagcgcgg ccacccccgg cgggttgccg accagcgcgt tggccagcgc
     1381 gagcgcgacc gcgtccatcg cgccgccggg actcagcccg aggtgttggt ggccgtgccg
     1441 gccccgatcc tgcaccgtgg tgagcaggcc cgggcgcagg acgcgcacgc tcattggcgt
     1501 tctcgcaggg aaaggaagtc gcgcttgtcg atcggccgga agcgcacgcg gtcgccggca
     1561 gagaacagac tcggctgcgg ccgtccggcg tcgaacgagg tcagcggcgt tcggccgatg
     1621 acgctccagc cgccgggcat ggagtatgga tagaccaccg tctgctggtt ggcgatcgcc
     1681 accgagcccg ccggcacgac cacgcgcggc tcggagcgcc gcggcaccgc aagcttcggg
     1741 tcgagcccgc cgatgtacgg atggcccggc gcgaagccga ccatcagcac gcggtactct
     1801 gcatccgcat gccgtttgat gatctcttca ggagagatct tcaatcgctg cgcaaggtcg
     1861 gccagatcca gcgcaaactc ggcgtcgtag cagatcggca cctcgactcc gcggcccaga
     1921 tcgtcggcgt cgggcaggcc cttcttcatg cattgctcga ccagctcggt ggccgctttc
     1981 aggacgtcgt cctcgcagat cgggtcgaaa tgcagcgcca cgccgccgag cgccggcacc
     2041 acgtcgcgga tccgcgggtc ggcgcgcgcg acgatcgccg ccgcgagccg ctgcgccacc
     2101 gagttcactt cgagatcgag tgtgcgggag aactcgacat atgccgcgag atcaccgagc
     2161 ggaaccacac ccaggggttt aggcagtcgg gccatttact tgggtaatgc gacgccgatc
     2221 attgaatccc ggttgacgag cgctgcgagg cgctcctcgc tccagccttc cgtactcgcc
     2281 ggacgcatag gcagcaccaa gtaacgcata tcggcggtgg agtcgtgcac gcggatggtg
     2341 acgtgctcgg gaatcttcag gccgaattcc tcgagcacga tgcggggctc gcacaccatg
     2401 cgcgagcggt agttgcgcga cttgtaccag tcaggcggga tgccgagcag cacgcgcggg
     2461 taacacgagc acagtgtaca tacgatcgcg ttgtgcacct gcggcgtgtt ctccaccacc
     2521 accagcttga gcgcggggat ctccaggccg agctcttcgc acgccttggt tccattcagc
     2581 agcaatcttt ccttgaaggc ggcatcgacc caggcgcgcg ccacgacctg cgcgccgcgc
     2641 tcgggcgtgc gctcacgcat ctcctcgacc tcgcggtcga cctgctgctc ggtgatgatc
     2701 cccttctcga ccagcagttc gcgcaaggtc acttccatca gctggaagta ggaaagcggc
     2761 tggtacttgc cctggtcgtc tttcacgcgc gatccagcca atgctcgaag atctcgacct
     2821 ccaccacgtc ggcatcgccg ccgcgatagt cgggccagac gtccttctgc ctgaagcgta
     2881 cgcggtacag cggaagcggc ggcaggccgc tgcgcatctg cgccagctcc tccgggttgg
     2941 cgaaggcgcc gcagatgcgc tcgacctcgc cgacgcagcc gcggatgtag tacggcgtgc
     3001 ggacgtggcc cagcggatac gccttcatca cgcgcacctt ctcccccggc ttgaacctag
     3061 cgctcgccac ggctctcgac ttccaccagc ttgcgcgcca tctcctcggt ggtgaggatg
     3121 ccgcgctgga tcagcgtctc ggcgagcgcg atgacccagc gctcgtagta ggtcatgtcg
     3181 tcataggcct tcggcggcag catctcgatc gcgcggcgcc gctcgtcgac cgtgatcagc
     3241 cgtttcgtgc ccggcttgat gccctgcagc agcaccgaca tcgcgtcgac gcgccgttcc
     3301 cacagctcgt agtcgtgctc cgtcggttcg acctttcccg ccggcaaccc gcccatgtcg
     3361 tggtggctgc gctcgatcat gcccggatgc tatcgcggcg caccatggcg tggatgccct
     3421 tgttgccgtc gaccagttgc gtgacgtgca ggtcgcaggc caggctgcag aacacgtagg
     3481 cctcggagcg cgtccagccc ttcatctcga ccagccagac gatcatgtcg cgcagcgcct
     3541 gcttggcggc gtcgtcgaga tccgtatcca ggccgagcgt gatgtagtgg gtcggcgtga
     3601 tggcgcgcgg catggcgagg ttcgtcttct tgtgcaccac cagctcgaag gtgcccttga
     3661 ggcaggtctc gagcgccgac aggcagacct cgccgtcgcc ctgcaccgcg tggccgtcgc
     3721 cgcacgagaa gttggcgccc ggcacgaaca ccggcaggaa cagcgacgtg ccggcgacgt
     3781 actccttgca gtcgacgttg ccgccgaact cgcgcggctc gaccgaggag atgcggccgt
     3841 actccgggcg cggcgcggta gcgaggatgc cgaagaacgg ctgcagcggg atggtcgggc
     3901 cccacggcag cgtcgcggtc atgttcttgc ggtcgatcgg cagcgtgcgc cgctcgaagg
     3961 ccgggaaatc ctcgggcagc gtgccgcgca gcggccggat cacgttccag ccccagtcta
     4021 ccgtgagctc gatcgccttg atccgcactt ccagcgtatc gccgggctgc gcgccgcgca
     4081 ccgcgaccgg cccggtgagg atgtgcgcgc ccagctcggg cttcagcttg gtgatgatct
     4141 cgcggtgcgc cggccggtag agactttcat cgggcgcgac gtcgcgccag ccggagacgg
     4201 tgtccaccac caccgtgtcg ccggactcca cctcgatgcg cgccggcagt cttgcgtcga
     4261 agtagcccca gtgcaccgtc tccggcgtcg gatcgaggcg atgcgtcttc aggcgaccca
     4321 ctccgacacc ggcgccctcg cctcctgcag cggctggcag acgatatcca ccagggacgg
     4381 cccgccatgc gaaagggctt ctttcaagac ctgtttgaga ttctcgggtt gaaaaacccg
     4441 aaatgccttg accccaaaag cctccgccac cttggcgtga tccgtcacgc cgaagtcgac
     4501 cgagaaatag cgctcgccgt agccggtctt ctgcccggcc ttgatccagc cgtagaccgc
     4561 gttcgagatg acgacgaagg tgatcggcag cttgaggcgc accgccgtct cgagctcgcc
     4621 gacgcacatg ccgaagctgc cgtcccccat caccgcgacg gtcttcacct tgggtcgcgc
     4681 gaagtgcgcg ccgatcgagg cggcaagcgc gtagccgagc gcgccatggg cgcggttcga
     4741 gaagtagcgc cgcccggtgc cgcgcaccag atagtaggcc gagaggtacg ggcagggtgt
     4801 cccagggtcg gccacgatga ttgaatcctt gcccaggact ttttgcagct cggcgacgac
     4861 gcgctcgggc ttgatcggcg aatcctccga cgccgccagg gtttcgaatt tcctgaactt
     4921 ctcgtctttc ttctggttta cgaacgaaag ctccctgggg gccgcgctca ccgcctcgtt
     4981 caacgcggcg aggaccagct tcgcgtctcc gatgagcggt acgtcgacct tgtagttcgt
     5041 gcccggcacc gccgggtcga cgtcgatgtg gatgatcttc gtcctgcccg gcgcgggatg
     5101 gcgccagcgc tcggtggtga cggagccggc acggcagccg acgaagatca ccaggtccgc
     5161 ggcgtccacc acggcgcgcg tctcgggcgt gccgccgttc gagccgacca cgccgatcga
     5221 cagcgcgcag cgctcgtcga tcgagccctt gccgctgatg gtggtcgcca ccggtgcgca
     5281 gatcttctcg gccagctcgg cgagctcggc ctccgcgccg gagagcacca cccctccacc
     5341 gcaaatgaaa atcggattct tcgcagttcg cagcagcttc gccgcaagct ccacaaacaa
     5401 cggatcgcaa gtggtgcgtc tggaagggta agaacctaga gtaggatcgg cccagacttc
     5461 agagcggtcc acgggaccat tctgaacatc gaaaggcaga gcgatgtgcg ccgcacccgg
     5521 gcggccggtg gtcatctcct cgaacgcctt cctgaagacg cgggggatgt cggcggagcg
     5581 gtcgagcacc gcgttccact tcgtcagcgg gcgcatcagg ttcttttgat ccagctcagt
     5641 caaagtaaaa cgaccacgcg acgaaaccga aatatccgtg ttgatggcga gaacg
//
LOCUS       DASYJA010000034         3043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1186949, whole genome shotgun sequence.
ACCESSION   DASYJA010000034 DASYJA010000000
VERSION     DASYJA010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186949"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..1468
                     /gene="soxC"
                     /locus_tag="VGO02_00750"
     CDS             212..1468
                     /gene="soxC"
                     /locus_tag="VGO02_00750"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HEV7476246.1"
                     /translation="MSVKRVAVGRRQFLKQGSAMLTGAAASAALAPGASAQNLAGEPW
                     ERVYGAPFTGYGERSRFEQPVMRHVLRPYGDLAPGSGAALSPIESLEGIITPSSLHNI
                     RSHSGTPDIDPKRHQLIVHGLVARPIKFSVEALSRYPMVTRTHFLECAGNSNRALTPQ
                     ALQVPAGALHGNMSCSEWTGIPLRVVLEEAGLLPQAKWLLAEGADSSHMSRSIPVEKA
                     LDDALIALYQNGERLRPEQGYPARLLLPGWEGNMSVKWLRRLKATDGPTYTKDETSKY
                     TDLLPDGRALQFTFEMGVKSVITKPSSTMTLPKGAISEISGLAWSGAGRIRRVEVSTD
                     GGASWADAALTGEERPRSFVRFRLPWEWKGGDAMLQSRATDEKGQVQPARRDWLAQYS
                     PGNPYHNNSIVTWSVGSDGGVKHVYA"
     gene            1455..2000
                     /locus_tag="VGO02_00755"
     CDS             1455..2000
                     /locus_tag="VGO02_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEV7476247.1"
                     /translation="MSTLKRSAAVVLLGVAACATPPQTAQRSPGLGVPVSAEELVAWD
                     ISIPPSGAGLPAGGGNARLGGPIFEQKCAVCHGAKGVGKPADLLVGGRGTLASKAPVR
                     TVGSYWQHATTLFDYVRRSMPITNPLSLTNDEVYAVSAYVLFLNGIVGEDEQMNAQSL
                     PRVAMPNRDGFIDDWRPRAVK"
     gene            complement(2015..>3043)
                     /locus_tag="VGO02_00760"
     CDS             complement(2015..>3043)
                     /locus_tag="VGO02_00760"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7476248.1"
                     /translation="MKLPAAAVLLAFAPVALGQTTRDVPAKNLPVPETVSPQIQKQIA
                     APLTPAWNVIPNTAAGWKEQVDAGYQATMKGLPALREALKVKVEPVTLGGVKAYMVTP
                     ESIAPQNRNRLLVHVHGGCFVSFPGESGTAEAIYMAGFGGFRVISVDYRMPPEHPYPA
                     GLDDAVSAWKEAVKMAPPKNMAIFGSSAGGNLTLAMVLRAKQEKLPLPAAIAPGTPMS
                     DLTGAGDSFQTNAMVDNVLVAYGANCDKRAALYAAGRDLRDPLLSPVYADVSGFPPAI
                     LTSGTRDLLLSNTVRMHRRLRQAGVEAVLQVFEGQSHAQYYRDPAAPETKEAFEEIAR
                     FFDKHLGR"
BASE COUNT          486 a         1009 c         1085 g          463 t
ORIGIN      
        1 agccgatcaa gggcacgccc gaggacttcg gcaaggtgat caaggccgag accgagaaat
       61 gggcgaaggt ggtgatcgcc tcgggcgcca aggtagacaa ctagacaggg cgtcgcggag
      121 tagcggtctt ttgcttgtaa acgccccttt ccagcgcgat tgcgcctagg ctgcgctctg
      181 actgcccgcg ctcgcgggcg aaggagcgca catgtcggtc aagagggtcg cggtcggtcg
      241 tcggcaattc ctgaagcagg gctctgcaat gttgacgggt gcagccgcct cggcggcgct
      301 cgcgcctgga gcaagcgcgc agaatctcgc cggggagccg tgggagcgtg tctatggcgc
      361 gccctttacc gggtatgggg agcgctcgcg cttcgagcag ccggtcatgc gccacgtctt
      421 gcggccgtac ggcgacctgg cccccgggtc gggcgcggcg ctctcgccga tcgagtcgct
      481 cgagggcatc atcaccccga gctcgctgca caacatccgc agccacagcg gcacgccgga
      541 catcgatccg aagcgccacc agctgatcgt gcacgggctg gtcgcgcgcc caatcaagtt
      601 ctccgtcgaa gccctgtcac gctacccgat ggtgacgcgg acgcacttcc tcgaatgcgc
      661 gggcaacagc aaccgggcgc tcacacccca ggcgctccag gttccggccg gcgccctgca
      721 cggaaacatg tcgtgcagcg aatggaccgg aatcccgctg cgcgtggtgc tggaagaggc
      781 cgggctgctg ccgcaggcaa agtggctgct tgccgaaggt gcagattctt cgcacatgag
      841 ccgcagcatc ccggtcgaga aggcactaga cgacgcgctg atcgcgctct accagaacgg
      901 cgagcggctg cggccggagc agggctaccc ggcgcgcctg ctgctgccgg gctgggaagg
      961 caacatgagc gtgaagtggc tgcgccggct caaggcgacc gatgggccga cgtacaccaa
     1021 ggacgagact tccaagtaca ccgacctgct gcccgacggc cgcgccctgc agttcacgtt
     1081 cgagatgggc gtgaaatcgg tgatcacgaa gccttcgtcc acgatgacgc tgccgaaagg
     1141 cgcgatcagt gagatttccg ggctcgcctg gagcggagcg gggcggatcc gccgcgtcga
     1201 agtgtcgacg gacggcggcg cgagctgggc ggacgcggcg ctcaccggcg aggagcgccc
     1261 aaggagtttc gtgcgcttcc gcctgccgtg ggagtggaaa ggcggcgacg ccatgctgca
     1321 aagccgcgct accgacgaga agggccaggt gcagccggcg cggcgcgact ggctggcgca
     1381 gtattcgccg ggcaacccgt accacaacaa ttcgatcgtg acctggtcgg tcggctccga
     1441 tgggggcgtc aaacatgtct acgcttaagc gcagcgcggc ggtggtgctg ctaggcgtcg
     1501 cggcgtgcgc cacgccgccg caaacggcgc agcgctctcc cggtctcggc gtgccggtca
     1561 gcgccgagga gctggttgcc tgggacatca gcatcccgcc gagcggagcc ggactcccgg
     1621 cgggcggcgg caacgcgcgc ctgggcggac ccatcttcga gcagaagtgc gccgtctgcc
     1681 acggagcgaa gggcgtgggc aagcccgccg acctgctggt cggcggccgc ggcacgctcg
     1741 cgtccaaggc gccggtgcgc acggtcggca gctattggca gcacgcgacg acgctcttcg
     1801 actacgtccg gcgctcgatg ccgatcacca atccgctctc gctcacgaac gacgaggtct
     1861 atgcggtgag cgcctatgtg ctcttcctga acggcatcgt cggcgaggac gagcagatga
     1921 acgcgcagag cctgccgcgc gtggcgatgc cgaaccggga cggcttcatc gacgactggc
     1981 gcccgcgcgc ggtgaagtag tcggcgagta ccggctaccg gcccaggtgc ttgtcgaaga
     2041 agcgggcgat ctcctcgaac gcttccttcg tctccggcgc ggctgggtcg cggtagtact
     2101 gcgcgtgcga ctggccttcg aagacctgca gcacggcttc gacgccggcc tggcgcagcc
     2161 tgcggtgcat ccgcacggta ttgctcagca gcaggtcgcg cgtgccgctg gtgaggatcg
     2221 ccggcggaaa gccgctcacg tcggcgtaca ccggcgagag cagcgggtcc ctgaggtcgc
     2281 ggcccgcggc gtagagcgcg gcgcgcttgt cgcagttggc gccgtaggcg acgagcacgt
     2341 tgtcgaccat cgcgttggtc tggaacgagt cgccggcgcc ggtgaggtcc gacatcggcg
     2401 tgcccggagc gatcgccgcg ggtagcggca gcttctcctg cttggcgcgc agcaccatcg
     2461 ccagcgtcag gttgccgccg gccgaggagc cgaagatcgc catgtttttc ggcggggcca
     2521 tcttcaccgc ctccttccag gccgagacgg cgtcgtcgag gccggccgga tagggatgct
     2581 cgggcggcat gcggtagtcg accgagatca ccctgaagcc gccgaagccc gccatgtaga
     2641 tggcctcggc ggtgcccgac tcgccgggga agctgacgaa gcagccgccg tgcacgtgca
     2701 ccagcaggcg gttgcggttc tgcggcgcaa tgctctccgg cgtcaccatg taggccttca
     2761 cgccgccgag cgtcaccggc tcgaccttca ccttgagcgc ctcgcgcagc gccggcagcc
     2821 ccttcatcgt cgcctggtag ccggcgtcga cctgctcctt ccagcccgcc gcggtattcg
     2881 ggatcacgtt ccaggccggc gtcagcggcg ccgcgatctg cttctggatc tgcgggctca
     2941 ccgtttccgg gaccggcagg ttcttggcgg ggacgtcgcg cgtggtctgg ccgagggcaa
     3001 cgggcgcgaa agcgagcaac acggcggcgg caggaagttt cat
//
LOCUS       DASYJA010000035         3009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_97160, whole genome shotgun sequence.
ACCESSION   DASYJA010000035 DASYJA010000000
VERSION     DASYJA010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97160"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VGO02_00765"
     CDS             complement(<1..448)
                     /locus_tag="VGO02_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV7476249.1"
                     /translation="MATRLFALLVLFGWGLAALAADPALLEELRKGGYVLYIRHTSTD
                     FSKDDTRMASFEDCANQRPLTDKGRDEARAIGSHLKRLGIPIDKVYASPFCRTVETAM
                     LAFGKAEKTQEVRGGPVRTDDPKRYDALKKLLSAPPASGQNNVVSSH"
     gene            complement(456..>3009)
                     /gene="acnA"
                     /locus_tag="VGO02_00770"
     CDS             complement(456..>3009)
                     /gene="acnA"
                     /locus_tag="VGO02_00770"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEV7476250.1"
                     /translation="VLRNCDGKKVSEQHIRELANWKAVAPRSDEIPFVVARIVLQDFT
                     GVPLLCDLAAMRGVAAKMGNDPKVIEPLVPVDLVVDHSVQIDHYGNKNALDLNMKLEF
                     KRNEERYQFMKWGMQAFDTFKVVPPGIGIVHQVNLEYLARGVHKSKEGVCYPDTLVGT
                     DSHTTMINGIGVVGWGCGGIEAEAGMLGQPVYFLTPDVVGVNLKGKLHPTVTATDLVL
                     TVTELLRKAKVVGKFVEFFGEGAASLTVTDRATISNMAPEYGATMGFFPVDDNTIEYF
                     RGTGRTDQEIEAFAAYYKAQGMYGIPKKGDIDYSQTLELDLSTIYPSLAGPKRPQDRI
                     DLDKVKSTFTGLFSGEIKNNGFGKKPEDLQRPYKTFDGIEIKNGDVLIAAITSCTNTS
                     NPGVLLAAGLLAKKAVELGLSVKPHIKTSLAPGSRVVTEYLTKAGLLPYLEKLGFNVA
                     AYGCTTCIGNAGPLAQPIEEAIVKNDMVCAAVLSGNRNFEARIHPNIRANFLASPPLV
                     VAYAIAGSVLRDFKTEPVGFTRDGKPVFIGHIWPGADEIKALMKLAMDAPTFRRLYSD
                     LANTNPMWKKVSAPAGQVYSWPKSTYIAEPPFFDGFSMTPRAPGDIRGAKILGVFGDS
                     VTTDHISPAGSIKPTSPAGIFLQEHDVAVADFNSYGARRGNHEVMMRGTFANVRIKNL
                     LLPGTEGGIALYKNEKMPIYDAAMHYIADGTPTVVFGGEEYGTGSSRDWAAKGTQLLG
                     VKAVIARSFERIHRSNLVGMGVLPLQFTGADSAASLGLKGDETIDVMLPADIQPQQDL
                     TLLIRNASGERRISVKSRIDTAIEVDYYKHGGILPFVLRELLGSAGKKARAA"
BASE COUNT          483 a          948 c         1004 g          574 t
ORIGIN      
        1 cgtggctcga gacgacgttg ttctgccccg acgcgggcgg cgccgacagc agcttcttca
       61 gcgcatcgta gcgcttcgga tcgtcggtcc gcaccggacc gccgcgcacc tcctgcgttt
      121 tctcggcctt gccgaaagcg agcatcgcgg tctcgaccgt ccgacagaac gggctcgcat
      181 acaccttgtc gatgggaatg cccaggcgct tgaggtggct gccgatggcg cgcgcctcgt
      241 cgcggccctt gtcggtgagc gggcgctggt tggcgcagtc ctcgaaggag gccatgcgcg
      301 tgtcgtcctt gctgaagtcc gtcgaggtgt ggcggatgta gagaacatag ccgcccttgc
      361 gcagctcctc gagcagcgcg gggtcggccg cgagcgcggc aagaccccag ccgaaaagga
      421 ccagcagcgc gaaaagcctg gtcgccacgg gcgcgctagg cggcgcgcgc cttcttcccg
      481 gcgctgccca gcaactcgcg cagcacgaaa ggcagaatgc caccgtgctt gtagtagtcg
      541 acctcgattg ccgtatcgat gcgcgatttg acgctgatcc gcctttcacc cgaggcgttc
      601 ctgatcagca gcgtcagatc ctgttgcggc tggatgtcgg cgggaagcat gacgtcgatc
      661 gtttcgtcgc ccttcaggcc gagcgaggcg gccgagtccg cgccggtgaa ctgcagcggc
      721 agcacgccca tgccgaccag gttggagcgg tggatgcgct cgaagctgcg cgcgatcact
      781 gccttcacgc cgaggagctg ggtccccttg gccgcccagt cgcgcgacga gccggtgccg
      841 tactcctcgc cgccgaacac caccgtcggc gtgccgtcgg cgatgtagtg catggcggcg
      901 tcgtagatcg gcatcttttc attcttgtac agggcgatgc cgccttcggt gccgggcagc
      961 agcaggttct tgatccgcac gttggcgaaa gtgccgcgca tcatcacctc gtggttgccg
     1021 cgccgcgcgc cgtagctgtt gaaatcggcc accgcgacgt cgtgctcctg caggaagatg
     1081 ccggcggggg aggtcggctt gatcgagccg gcgggcgaga tgtggtcggt ggtcaccgag
     1141 tcgccgaaca cgccgaggat cttcgcgccg cggatgtcgc ccggggcgcg gggtgtcatc
     1201 gagaaaccgt cgaagaacgg cggctcggcg atgtaggtcg acttcggcca gctgtagacc
     1261 tgcccggcgg gggccgacac cttcttccac atcgggttgg tgttcgccag gtcggagtag
     1321 aggcggcgga aggtgggggc atccatcgcc agcttcatca gcgccttgat ctcgtcggcg
     1381 cccggccaga tgtggccgat gaaaaccggc ttgccgtcac gggtaaaacc aaccggctcc
     1441 gtcttgaaat ccctcagcac cgaacctgca atcgcgtacg ccacgaccag cggcggcgac
     1501 gccaggaagt tcgcgcggat attaggatgg atgcgcgcct cgaaattgcg atttccggag
     1561 aggacggccg cgcacaccat gtcgttcttg acgattgcct cctcgatcgg ctgcgcgagc
     1621 gggccggcgt tgccgatgca ggtggtgcag ccgtaggcgg cgacgttgaa gccgagcttc
     1681 tcgaggtagg gcagcaggcc cgccttcgtc aggtactccg tcaccacgcg cgagcccggc
     1741 gcgagcgagg tcttgatgtg cggcttcacc gacagcccga gctcgaccgc cttcttggcg
     1801 agcaggcccg cggcgagcag cacccccggg ttcgaggtat tggtgcacga ggtgatcgcg
     1861 gcgatcagca cgtcgccgtt cttgatttcg atgccgtcga aggtcttgta ggggcgctgc
     1921 aggtcttcgg gttttttccc gaatccatta tttttgattt ccccggaaaa aagacctgta
     1981 aaggttgact tcaccttgtc cagatcgatg cggtcctgcg gccgcttggg gcccgcgagc
     2041 gacggataga tcgtcgacag atcgagctcg agggtctgcg agtaatcgat gtcgcccttc
     2101 ttcgggatgc cgtacatgcc ctgcgccttg tagtaggcgg cgaaggcctc gatctcctgg
     2161 tcggtgcggc cggtgccgcg gaaatactcg atggtgttgt cgtcgaccgg gaagaaaccc
     2221 atggtggcgc cgtactcggg cgccatgttg gagatggtgg cgcggtcggt gacggtgagc
     2281 gacgcggcgc cttcgccgaa gaactcgacg aacttgccca cgactttcgc ttttctcaag
     2341 agttctgtca cggtcaaaac caggtctgta gcagtgaccg tgggatgcag cttgcccttc
     2401 aggttgacgc cgacgacgtc cggcgtgagg aaatacaccg gctggccgag catgccggcc
     2461 tcggcctcga tgccgccgca gccccagccg actacgccga tgccgttgat catggtggtg
     2521 tggctgtcgg tgccgaccag ggtgtcggga tagcagacgc cttccttcga cttgtgcacg
     2581 ccgcgcgcca gatactcgag gttgacctgg tgcacgatgc cgattcccgg cggcaccacc
     2641 ttgaaggtgt cgaaggcctg catgccccac ttcatgaact ggtagcgctc ctcgttcctc
     2701 ttgaattcca gcttcatgtt gaggtcgagc gcgttcttgt tgccgtagtg gtcgatctgc
     2761 accgaatggt cgaccaccag gtcgaccgga accagcggct cgatcacctt cgggtcgttg
     2821 cccatctttg ctgccacgcc gcgcatcgcc gcgaggtcgc agagcagggg cacgccagtg
     2881 aagtcctgca gcacaatgcg cgcgacgacg aacgggatct cgtcgctgcg cggcgcgacc
     2941 gccttccagt tggcgagctc gcggatgtgc tgctcgctca ccttcttgcc gtcgcaattg
     3001 cgcagcacc
//
LOCUS       DASYJA010000036         2192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3093272, whole genome shotgun sequence.
ACCESSION   DASYJA010000036 DASYJA010000000
VERSION     DASYJA010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3093272"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..176
                     /locus_tag="VGO02_00775"
     CDS             18..176
                     /locus_tag="VGO02_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEV7476251.1"
                     /translation="MPLAMLLGAAFLLAVDTLCRTIATIEVPPGVLTALIGTPFFLWL
                     FAMSRRSW"
     gene            176..949
                     /locus_tag="VGO02_00780"
     CDS             176..949
                     /locus_tag="VGO02_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7476252.1"
                     /translation="MLQVEALAFGFPGRTIGRDVGFSLGAGEVMCVLGPNGGGKTTLF
                     RTLLGLLASHGGQILLEERPIETLSRSEIARLVGYVPQGHSGYFAYTVRDFVLMGRTA
                     HLGIFSSPSKKDFDVADRALASLGMAHLAGRPITEVSGGERQLALVARALAQEPRLLV
                     LDEPTANLDFGNQVRVLERIAALAQTGISILFSSHDPDHAFLCAQRALLLAEGRALEI
                     GAPRDVIRADTLQRLYGVSVEVLPLAGGGHTCLPAITPR"
     gene            922..>2192
                     /gene="chrA"
                     /locus_tag="VGO02_00785"
     CDS             922..>2192
                     /gene="chrA"
                     /locus_tag="VGO02_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEV7476253.1"
                     /translation="MSAGNYTPVAFAEAFRVWLKIGCLGFGGPAGQIALMHRILVDEK
                     KWVEESRYLHALNFCMLLPGPEAQKLATYVGWLLHGLRGGLAAGILFVLPGALVMLVM
                     SLLYVLGKGVPAVDGALFGIKAAVLVIVVEALLRIGRRALKSRLLVGLAAAAFIGIFF
                     LDLPFPLIVAASAAIGYFTTQDRGAASGAPPGRWRAFALTVLFGLALWIAPTALVLLA
                     LGKGHVLVDIGAFFSKLAAVSFGGAYALLAYMAQEAVQTYHWMSAPEMVDGLGLAETL
                     PGPLIKVTQFVGFLAAYRDPLPFSPVVAGVLGSALTTWVTFAFPMLLIFALAPFVEAL
                     RANQRLSGALAAVTAAVVGVILNLTVWFALHVLFARVEEIRAGPLRAYVPELATLDWQ
                     AAALALVAAALMLRMHRGLVETVAVMATLGIL"
BASE COUNT          273 a          792 c          718 g          409 t
ORIGIN      
        1 cgaacatcgg ccgcctgctg ccgctagcca tgctgctcgg cgcggcgttc ctgctcgccg
       61 tggacacgct gtgccgcacc attgccacca tcgaggtgcc gcccggcgtg ctgacggcgc
      121 tgatcggcac gccgttcttc ctctggctgt tcgccatgtc gcggcggagc tggtagtgct
      181 ccaggtcgaa gcgctcgcct tcggcttccc cggccgcacc atcgggcgcg acgtcggctt
      241 ctcgctcggc gcaggcgagg tgatgtgcgt gctcgggccg aacggcggcg gcaagaccac
      301 gctatttcga acgttactcg gccttctcgc ttcccacggt ggacaaatcc tcctggaaga
      361 aagacctatc gaaacccttt caaggagcga aatcgccagg ctggtcggct acgtgccgca
      421 ggggcacagc ggctatttcg cctacacggt gcgcgacttc gtgctgatgg ggcgcaccgc
      481 gcacctcggc atcttttcct cgccttcgaa aaaagacttc gacgtcgccg atcgggccct
      541 ggcctcgctc ggcatggcgc acctcgccgg gcggccgatc accgaggtct ccggcggcga
      601 gcgccagctc gcgctggttg cgcgcgccct cgcccaggag ccgcgcctgc tggtgctcga
      661 cgagccgacc gcgaacctcg acttcggcaa ccaggtgcgc gtgctggaga ggatcgccgc
      721 gctggcgcag accggcatct cgatcctgtt ctccagccac gacccggacc acgctttcct
      781 ctgcgcacag cgcgcgctgc tcctcgccga gggccgcgcc ctcgagatcg gcgcgccgcg
      841 cgacgttatc cgcgccgaca cgctgcagcg cctctatggc gtttcggtgg aagtcctgcc
      901 gctcgccggc ggaggccata cgtgtctgcc ggcaattaca ccccggtagc cttcgcggaa
      961 gcattccggg tctggctgaa gatcggctgc ctgggcttcg gcgggccggc cggccagatc
     1021 gcgctgatgc accgcatcct ggtcgacgag aagaaatggg tcgaagagag ccgctacctg
     1081 cacgcgctca atttctgcat gctcctgccc ggaccggagg cgcagaagct cgccacctac
     1141 gtcggctggc tcctgcatgg cctgcgcggc ggccttgccg ccgggatcct gttcgttctg
     1201 cccggcgcgc tggtcatgct cgtcatgagc ctgctgtacg tgctgggcaa aggcgtcccc
     1261 gcggtcgacg gcgcgctgtt cggcatcaag gccgcggttc tcgtcatcgt agtcgaggcg
     1321 ctgctgcgca tcggccgccg cgcgctgaag tcgcggctcc tggtcggcct ggccgccgcc
     1381 gcgttcatcg gcatcttctt cctcgacctg ccgtttccgc tgatcgtcgc cgccagcgcc
     1441 gcaatcggat acttcaccac gcaggatcgc ggcgccgcct ctggcgcgcc gccgggacgc
     1501 tggcgtgcct tcgccctcac cgtgcttttc ggcctcgcgc tctggatcgc cccaaccgcg
     1561 ctggtgctgc tcgcgctcgg caaggggcat gtgctggtcg acatcggcgc gttcttctcc
     1621 aagctcgccg ccgtcagctt cggcggcgcc tacgcgctgc ttgcctacat ggcgcaggag
     1681 gccgtgcaga cctaccactg gatgagcgca ccggagatgg tggacgggct gggtctcgcc
     1741 gaaacgctac ccggcccgct gatcaaggtc acccagttcg tcggctttct cgcagcctat
     1801 cgcgatccgc tgccgttctc gccggtcgtg gcgggcgtgc ttggctctgc cctgaccacc
     1861 tgggtgacat tcgccttccc catgctgctg atcttcgccc tggccccgtt cgtcgaggcg
     1921 ttgcgcgcca accagcggct gtccggggcc ctcgccgcgg tcaccgcggc ggtggtcggg
     1981 gtcatcctca acctgactgt ctggttcgcg ctgcacgtgc tgttcgcccg cgtcgaggaa
     2041 atccgcgccg ggccgctgcg cgcctacgtg ccggaactgg cgacgctcga ctggcaggcg
     2101 gcggcgctgg ccctggtggc ggccgcgctc atgctgagga tgcatcgcgg cctggtcgaa
     2161 acggttgccg tgatggcgac tctgggtatc ct
//
LOCUS       DASYJA010000037         2867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2912359, whole genome shotgun sequence.
ACCESSION   DASYJA010000037 DASYJA010000000
VERSION     DASYJA010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2912359"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="VGO02_00790"
     CDS             <1..1052
                     /locus_tag="VGO02_00790"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEV7476254.1"
                     /translation="MCIGGTSQWPATIFRGIDQWGERYGYILVDPIGGAIGAFSHADG
                     ISTGGQARTPICKLPNVEHTEQSFPVLFLYRKEITDSGGAGKYRGGLSAESCFIPHGT
                     DSITHDTLSSGNAIPTSTGMMGGYPGAVNRYRFLKESNVLEKMKQSELVNDIDELKGK
                     KEELQLRQQDFTQNPSDVYAVLWSAAGGFGDPLDRDPAKVEADVANGDVSETAARKIY
                     GVILDNKEQTEKERAARRKQRLTKATAPFAPAKTLEGRERLRATESLAVRGANWCCAK
                     CAADLGPMKGNYKERCARSDQPIQASNPLVGDPRRFIDPLPQFRQFCCPGCGALIENE
                     IALAEDPVLRDVEIR"
     gene            1036..2211
                     /locus_tag="VGO02_00795"
     CDS             1036..2211
                     /locus_tag="VGO02_00795"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7476255.1"
                     /translation="MSRFAEVGYDEAMRRAREIVPVLMERAQKCEDARVLLPENEKLL
                     HETGLFRYHQPRRFGGMELPFIAVVDIVAELARGCPSTAWNVGNLGCHHWILAYYEPA
                     TQHEVWDANPDVLIASSIALAAGRGRKTKDGFVVSGKWPFSSGVDNSDWNMLAVTIYE
                     DEKPVDWRLCLVPKPDYKVIDTWYAMGMVGTGSKDIEVKEVFVPERRALALARCRGGL
                     EHPGAALNKGELFRVPIVASAGHPLAAAALGAAEGAYDHVVEKFKSRVGTYTGAKVSD
                     FQAVQIRIARARALIDSARYLMRESALVFQNNEKLEIEKKLRLRTQNAFAVHQCHEAV
                     EALWQCYGANAIYTRDPLQRFQRDLQAMKQHFSFNFDIAGAAFGTVALGGKYTNPTL"
     gene            complement(2201..2545)
                     /locus_tag="VGO02_00800"
     CDS             complement(2201..2545)
                     /locus_tag="VGO02_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEV7476256.1"
                     /translation="MLCHCRTCRRVAGAPVVGWVTFPLASFRFIGGEPDEFQSSPPVL
                     RTFCRQCGTPLTYRNESESETLDVTTATLDDPNAYPPTHHSWLSHDLAWVKFGDGLPT
                     FPESRPKQEPTT"
     gene            complement(2620..>2867)
                     /locus_tag="VGO02_00805"
     CDS             complement(2620..>2867)
                     /locus_tag="VGO02_00805"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV7476257.1"
                     /translation="PGVTTRWHRVKDTAERYVILQGSGRVEVGDLPPQEVGPGDVVLI
                     PPSVRQRIANVGDVDLILLAICTPRFRDAAYEDLEKR"
BASE COUNT          535 a          951 c          940 g          441 t
ORIGIN      
        1 tcatgtgcat cggcggcacc agccagtggc cggcgaccat cttccgcggc atcgaccagt
       61 ggggcgagcg ctacggctac atcctggtcg acccgatcgg cggcgccatc ggcgccttct
      121 cgcacgccga cggcatctcg accggcggcc aggcgcgcac gccgatctgc aagctgccca
      181 acgtggagca cacggagcag agctttccgg tcctgtttct gtatagaaaa gagatcacgg
      241 attccggcgg cgccgggaag taccgcggcg gcctgtcggc cgagtcctgc ttcatcccgc
      301 acggcaccga cagcatcacg cacgacacgc tgtcctccgg caacgccatc ccgacctcca
      361 ccggcatgat gggcggctat cccggcgccg ttaaccgcta ccgctttctg aaagaaagca
      421 atgttttaga aaagatgaag caaagcgagc tggtcaacga catcgatgag ctgaagggaa
      481 agaaagagga gcttcagctc cgccagcagg atttcacgca gaacccgagc gacgtctacg
      541 ccgtgctgtg gtcggcggcc ggcggcttcg gcgatccgct ggaccgcgat ccggccaagg
      601 tcgaggccga cgtggcgaat ggcgacgtgt cggagactgc ggcgcgcaaa atctatggcg
      661 tcattctcga caataaggag caaacggaaa aggaaagagc cgccaggcgc aagcagcgcc
      721 tgacaaaggc caccgcgccg ttcgccccgg cgaagacgct cgagggcagg gagcgcctgc
      781 gcgccaccga aagcctcgcg gtgcgcggcg ccaactggtg ctgcgcgaaa tgcgccgccg
      841 acctcggccc gatgaagggc aactacaagg agcgctgcgc gcgcagcgac cagccgatcc
      901 aggcctcgaa cccgctggtc ggcgatccac ggcgcttcat cgacccgctg ccgcagtttc
      961 gccagttctg ctgccccggc tgcggcgcgc tgatcgagaa cgagatcgcc ctcgccgagg
     1021 acccggtgct gcgggatgtc gagattcgct gaggtcggct acgacgaggc gatgcgccgg
     1081 gcgcgcgaaa tcgtacccgt gctcatggag cgcgcgcaga agtgcgaaga cgcgcgcgtg
     1141 ctgctgcccg aaaacgagaa gctgctgcac gagacgggcc tgttccgcta ccaccagccc
     1201 aggcgcttcg gcggcatgga gctgcctttc atcgcggtcg tggacatcgt cgcggagctg
     1261 gcgcgcggct gcccctcgac ggcctggaac gtcggcaacc tcggctgcca ccactggatc
     1321 ctcgcctact acgagcccgc gacgcagcac gaggtgtggg acgcgaatcc cgacgtcctg
     1381 atcgcctcct cgatcgcgct ggcggcggga cgggggcgca agaccaagga cggcttcgta
     1441 gtcagcggca agtggccgtt ctcctcgggc gtggacaatt ccgactggaa catgctggcc
     1501 gtcaccatct atgaagatga gaagccggtc gactggcggc tgtgcctcgt gcccaagccg
     1561 gactacaagg tgatcgacac ctggtacgcg atggggatgg tcggcaccgg cagcaaggac
     1621 atcgaagtga aggaggtctt cgtccccgag cgccgcgcgc tggccctggc ccgctgccgc
     1681 ggcgggctcg agcatcccgg cgcggcgctg aacaagggcg agctcttcag ggtgccgatc
     1741 gtcgcctccg cgggacatcc cctcgccgca gccgccctcg gcgcagccga gggcgcgtat
     1801 gaccacgtcg tcgaaaaatt caagagtcgt gtggggactt ataccggcgc aaaggtatcg
     1861 gacttccagg cggtgcagat ccgtatcgcg cgggcgcggg ccttgatcga ttcggcacgc
     1921 tatctcatgc gcgaatccgc cctggtgttt cagaacaacg aaaagctgga gatcgagaaa
     1981 aagctgcgcc tgcgcacgca gaacgccttt gccgtgcacc aatgccacga agctgtggaa
     2041 gcactatggc agtgctacgg cgcgaacgcg atctacaccc gcgacccgct gcagcgcttc
     2101 cagcgcgacc tgcaggcgat gaagcagcat ttctcgttca acttcgacat cgccggcgcc
     2161 gctttcggca cggtcgccct cggcggcaag tacaccaacc ctacgttgta ggttcctgtt
     2221 tagggcgcga ctcgggaaag gtcggcagac cgtcgccgaa tttcacccat gccaggtcgt
     2281 ggctgagcca ggagtgatgc gtcggcggat aggcattcgg atcgtcgagc gtcgcggtag
     2341 taacatcgag cgtctccgac tcgctctcgt tgcggtaggt gagcggcgtg ccgcactgcc
     2401 ggcaaaaggt gcgcagcacc ggcggcgagg actggaattc gtcgggctcg ccgccgatga
     2461 agcggaagct cgcgagcggg aacgtgaccc agccgacgac cggcgcgccc gccacgcggc
     2521 ggcaggtgcg gcaatggcaa agcatgctcg ccgtaggcgc aacttctgcc cggtagcgga
     2581 tcgctccgca aaaacagccg ccttcgatgc tcgattcgct catcgcttct ccagatcctc
     2641 gtaggctgcg tcgcgaaagc gcggcgtaca gattgccagc aagatcaggt ccacgtcgcc
     2701 cacgttcgca atccgctgcc tgaccgacgg cgggatcagc accacgtcgc ccggcccgac
     2761 ttcctgcggc ggcaggtcgc ccacttcgac gcggccgctc ccctgcagga tgacgtagcg
     2821 ctccgcggtg tcctttacgc gatgccaacg cgtggtgacg cccggcg
//
LOCUS       DASYJA010000038         2576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_733464, whole genome shotgun sequence.
ACCESSION   DASYJA010000038 DASYJA010000000
VERSION     DASYJA010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733464"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VGO02_00810"
     CDS             complement(<1..576)
                     /locus_tag="VGO02_00810"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7476258.1"
                     /translation="MSQQKYTRASPSPRYRRLIEQYQQMHLHGDQHTGVPPERTFPGE
                     SLPRQAPHIKRLIRLTGASTLLDYGCGKGQQYLPRRTADPDEGVEYPDMQSYWGVQAI
                     QRYDPAYQPFTQLPSGTFDGVICTDVLEHCPEEDIPWILGELFGYATRFVFANVACFP
                     AKKRLPSGGNAHCTIKPVRWWEEHVERAARAK"
     gene            complement(573..989)
                     /locus_tag="VGO02_00815"
     CDS             complement(573..989)
                     /locus_tag="VGO02_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEV7476259.1"
                     /translation="MECTIRWTGGMTFLAETGSNHIVAMDGAPEGGGRNLAPRPMEMV
                     LVGTGGCAAYDVVVILKKSGQEVTGCEVKLSSERAPNDPKVFTKIHMHFEVSGRGLKR
                     NLVEHAIRLSHEKYCSASIMLGKTAEITKDFAIIEE"
     gene            1228..1656
                     /gene="rplM"
                     /locus_tag="VGO02_00820"
     CDS             1228..1656
                     /gene="rplM"
                     /locus_tag="VGO02_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEV7476260.1"
                     /translation="MKTYTAKTGEVQHGWYVVDAQNKVLGKLASQIASRLRGKHKPEY
                     TPHVDTGDFIVVVNVSKLRVTGRKDERKIYYRHSGYPGGIKQDTFAKLHATKPERVLK
                     KAVKGMLPKGPLGYAMLSKLKLYAGGTHPHSAQQPKALEI"
     gene            1658..2050
                     /gene="rpsI"
                     /locus_tag="VGO02_00825"
     CDS             1658..2050
                     /gene="rpsI"
                     /locus_tag="VGO02_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEV7476261.1"
                     /translation="MVGEYYYGTGRRKSAVARVFLKPGKGDFVVNGKPVDDFFGRETG
                     RMVVRQPLELIKQLANFDILVNVYGGGESGQAGAVRHGITRALIEYDGALKPQFSAAG
                     LVTRDAREVERKKVGLHKARRRKQYSKR"
     gene            2122..>2576
                     /locus_tag="VGO02_00830"
     CDS             2122..>2576
                     /locus_tag="VGO02_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEV7476262.1"
                     /translation="MIKAGIVGGTGYTGVELLRLLAQHPQVELKAITSRKEAGTPVAA
                     MFPSLRGRVELAFIEPSREALRGCDVVFFATPNGVAMGDARAMLDAGARVMDLSADFR
                     IQDLAEWERFYKTKHAAPELVRDAVYGLCEVNRERIRGAWLVANPGCYPT"
BASE COUNT          444 a          792 c          871 g          469 t
ORIGIN      
        1 cttggcgcgc gcggcgcgct cgacatgctc ttcccaccag cgcaccggct tgatggtgca
       61 gtgcgcgttg ccgccgctcg gcaagcgctt ctttgccgga aagcaggcaa cgttggcgaa
      121 tacgaatctg gtcgcgtagc cgaaaagctc gccgaggatc cagggaatgt cctcctcggg
      181 acagtgctcg aggacgtcgg tgcagatcac gccgtcgaaa gtgccgctcg gcagctgcgt
      241 gaacggctgg taggccgggt cgtagcgctg gatcgcctgc acgccccagt aggactgcat
      301 gtccgggtac tcgacgccct cgtccgggtc ggcggtgcgc cgcggcaggt actgctggcc
      361 tttgccgcag ccgtagtcga gcagagtcga ggcgccggtg agcctaatga gccgcttgat
      421 gtgtggcgcc tggcgcggca ggctctcgcc cgggaaggtg cgctcgggcg gcacgcccgt
      481 gtgctggtcg ccgtgcaggt gcatctgctg gtactgctcg atgaggcggc ggtagcgcgg
      541 gctgggcgag gcgcgggtgt atttctgctg actcattctt cgatgatagc gaagtccttg
      601 gtgatctccg ccgttttgcc gagcatgatg gacgccgagc agtacttctc gtgcgacagg
      661 cggatcgcat gctccaccag gttgcgcttg aggccgcggc cgctcacctc gaagtgcatg
      721 tggatcttgg tgaagacctt cggatcgttc ggcgcgcgct cggagctgag cttcacctcg
      781 cagccggtca cctcctggcc gcttttcttc aggatcacca ccacgtcgta ggcggcgcag
      841 ccgccggtgc ccaccagcac catctccatg gggcgcggcg cgaggttgcg gccgccgccc
      901 tcgggcgcgc cgtccatcgc gacaatgtga ttgctgccgg tttcggccag aaaagtcatg
      961 ccgccggtcc agcgaatggt gcattccatg gcagtttcct tgccgatcaa tgggttgtgc
     1021 tatgcagcat ggacgcccgg ctcccagaat gagaaaccgg gtattgcatc gcagcaatcg
     1081 ggttctgcat aatgccgatt gtagtcgtct cctcgagcct cctccttttt ggtggattcg
     1141 gcgcaactcg cgagagctgc gccctttttt tgggtacaat ccacggccct tatttatgcc
     1201 tttttggcaa ttccggcacg gacttacatg aaaacttata cggcgaagac aggcgaggtc
     1261 cagcacggct ggtatgtcgt cgatgcccag aacaaggtcc tcggcaagct ggcgagccag
     1321 atcgcttcgc gcctgcgcgg caagcacaag cccgaataca ccccgcacgt cgacaccggc
     1381 gacttcatcg tcgtggtgaa cgtgtccaag ctgcgcgtta ccggccgcaa ggacgagcgc
     1441 aagatctact atcggcacag cggctatccg ggcggcatca agcaggacac ctttgccaag
     1501 ctgcatgcga cgaagcccga gcgcgtgctc aagaaggcgg tgaaaggcat gctgcccaag
     1561 ggtcctctcg gctacgcaat gctgagcaag ctgaagctct acgccggcgg cacgcatccc
     1621 cattcggcgc aacaaccgaa agcattggag atctagcatg gtcggtgagt actactacgg
     1681 cacgggaagg cgcaagagcg cggtggcgcg cgtgttcctc aagccgggca agggcgattt
     1741 cgtcgtcaac ggcaagccgg tggacgattt cttcggccgc gagaccggcc gcatggtggt
     1801 gcgccagccc ctcgagctga tcaagcagct ggcgaacttc gacatcctgg tcaacgtcta
     1861 cggcggcggc gagagcggcc aggccggcgc ggtgcgccac ggcattacgc gggcgctgat
     1921 cgagtatgat ggggcgctga agccgcagtt cagcgccgcc ggactggtga cgcgcgatgc
     1981 gcgagaggtg gagcgaaaga aggtcggact gcacaaggcg aggcggcgca agcagtactc
     2041 gaaacgctaa tcggagaatc tggcttcacc gatcgaaagc cgccttaggg cggctttctt
     2101 gtttctggag ttgattgact catgatcaag gcgggaatcg tcggcggtac cgggtacacg
     2161 ggcgtggagc tgctgcggct gcttgcgcag catccgcagg tcgagctgaa ggccatcact
     2221 tcgcgcaagg aggccgggac accggtcgcg gcgatgttcc ccagcctgcg cgggcgcgtc
     2281 gagctggcgt tcatcgagcc aagccgcgaa gcgctgcgcg gctgcgacgt cgtgttcttc
     2341 gccacgccga acggcgtcgc catgggcgat gcgcgcgcga tgctcgacgc cggcgcgcgc
     2401 gtgatggacc tgtcggccga tttccgcatc caggacctcg ccgagtggga gcgcttctac
     2461 aagacgaagc acgccgcgcc ggagctggtg cgcgacgcgg tgtacggcct gtgcgaggtg
     2521 aaccgcgagc gcatccgcgg cgcgtggctg gtcgcgaacc ccggctgcta tccgac
//
LOCUS       DASYJA010000039         2143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1278238, whole genome shotgun sequence.
ACCESSION   DASYJA010000039 DASYJA010000000
VERSION     DASYJA010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278238"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="VGO02_00835"
     CDS             <1..871
                     /locus_tag="VGO02_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV7476263.1"
                     /translation="AGGELLGALSFGGPSAAFPKEQLAIVREVAAQLAIALAQARLLA
                     RVKAHAAELESRVQERTAELEATNKELESFSYSVSHDLRAPLRAVDGYARMLEEDHGA
                     RLDEEGRRLLGVVRAASVRMGKLIDDLLAFSRLGRQAPQRRLLDMTALAREVLAELNG
                     AGLARIELAALPAASADPALLRQVWSNLIGNALKYSSKRSDARVQIGGDQDPEESVYW
                     VRDNGVGFDPRYAAKLFGVFQRLHRAEEFPGTGVGLAIVQRVMARHGGRVWAESTLGE
                     GACFYFSLPREAA"
     gene            868..>2143
                     /locus_tag="VGO02_00840"
     CDS             868..>2143
                     /locus_tag="VGO02_00840"
                     /inference="COORDINATES: protein motif:HMM:NF028467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HEV7476264.1"
                     /translation="MSELALASPGLYPGRTLLSTLPAASAEQRRALAVVLISTAIFAA
                     VAPFAKLQLAPVPAFLPAYQAALIVNDLITAVLLLGQFGILRWRALLFLAGAYLFSAF
                     MAVAHALSFPGLFLPSGLLGVGPQTTAWIYFLWHAGFPLLVIAYALSPHDGKAARVPG
                     DARLAILGCIGMVLALTCGLTLATTAGHDLLPTIMAGNRDAPAKVVVATAAWGFSVAA
                     LPLLWRRRPHSVLDLWLMVVMCVWVFDIALASVLNAGRFDLGWYAGRIYGLLAASFVL
                     IVLLLENGVLYARLSQAYAAEQRERARAEQRSAELDAANKELDAFSHSVSHDLRAPLR
                     AVDGFAAMLEEDYAERLDDEGRRRLKALRLGCTQMGRLIDDLLELSHAGRTEVRKWRI
                     DMAALAREVAAQVPDASRARIDIPDLPQANGDR"
BASE COUNT          281 a          741 c          766 g          355 t
ORIGIN      
        1 cgccggcggc gagctgctcg gcgcgctgag cttcggcggg ccgagcgccg cgtttccgaa
       61 ggagcagttg gcgatcgtgc gcgaggtggc ggcgcagctc gcgatcgcgc ttgcgcaggc
      121 gcgcctgctg gcgcgcgtga aggcgcacgc ggccgagctc gaatcgcgcg tgcaggagcg
      181 caccgccgag ctcgaggcca ccaacaagga gctcgagagc ttcagctact cggtatcgca
      241 tgacctgcgc gcgccgctgc gcgcggtgga cggctacgcg cgcatgctcg aggaggacca
      301 cggcgcgcgc ctggacgaag agggccggcg cctgctcggc gtggtgcgcg cggcgagcgt
      361 gcggatgggc aagctgatag acgatcttct cgccttctcg cggctcggcc gccaggcgcc
      421 gcaacgccgg ctgctcgaca tgaccgcgct cgcgcgcgag gtgctagccg agctcaacgg
      481 ggcaggactt gctcggatcg agcttgctgc gctgcctgcg gcgagcgccg acccggcgtt
      541 gctcaggcag gtgtggagca acctgatcgg caacgcgctc aaatactcga gcaagcgctc
      601 cgatgcgcgc gtccagatcg gcggcgacca ggacccggag gagagcgtgt actgggtgcg
      661 cgacaacggc gtcggcttcg acccgcggta tgcggcgaag ctcttcggcg tgttccagcg
      721 tctgcaccgg gccgaagagt ttccgggcac cggcgtcggc ctggcgatcg tgcagcgcgt
      781 gatggcgcgc cacggcgggc gcgtctgggc cgagagcacc ctcggcgagg gcgcgtgctt
      841 ctacttttcg ctgccccggg aggcggcatg agcgagttgg cgctcgcaag tcccggcctg
      901 tacccaggcc gcacgctgct gtcgaccttg cctgcggcat ccgcggagca gcgcagggcg
      961 ctcgcggtcg tgctgatctc cacggcgatc ttcgccgcgg tggcgccctt cgccaagctg
     1021 cagcttgcgc cggtgccggc cttcctgccc gcctatcagg ccgcgcttat cgtcaacgat
     1081 ctcatcacgg cggtgcttct cctcggccag ttcggcatcc tgcgctggcg cgcgctgctc
     1141 tttctggcag gcgcgtacct cttcagtgcc ttcatggccg tagcccatgc gctcagcttc
     1201 ccgggcctgt tcctgccctc cggcctgctc ggcgtcgggc cgcagacgac ggcgtggatc
     1261 tacttcttgt ggcatgccgg gtttccgctc ctggtcatcg cgtatgccct ctcgccgcac
     1321 gacggcaagg cggcgcgggt ccccggcgat gcgcgcctcg cgatcctggg ctgcatcggc
     1381 atggtcctcg cgctcacttg cggactgacg ctcgccacta ccgcgggtca cgacctcctg
     1441 ccaacgatca tggcgggcaa ccgcgacgcg ccggcgaaag tcgtcgtcgc caccgccgcc
     1501 tggggattca gcgtggccgc gctgccgctc ctgtggcgcc gccgcccgca ctcggtgctg
     1561 gacctctggc tgatggtggt gatgtgcgtc tgggtcttcg acatcgctct cgcctccgtg
     1621 ctcaatgccg ggcgcttcga tctcggctgg tatgcgggcc gcatctacgg gcttctcgcc
     1681 gcgagcttcg tgcttatcgt tctgcttctg gaaaacggcg tgctatacgc ccgcctttcc
     1741 caggcgtacg cggcggagca gcgcgagcgc gcccgcgcgg agcaaaggag cgcggagctg
     1801 gacgcggcga acaaggagct cgacgcgttc tcgcattccg tgtcgcacga cctgcgcgcc
     1861 ccgttgcggg cagtggacgg cttcgccgcc atgctggagg aagactacgc cgagcggctc
     1921 gacgacgagg ggcggcgcag actgaaagcg ctgcgcctgg gctgcacgca gatggggcgg
     1981 ctgatcgacg acttgctcga actgtcgcat gccgggcgca ccgaggtgcg caaatggcgg
     2041 atcgacatgg cggcccttgc gcgcgaggtg gctgcgcagg tgcccgacgc cagccgcgcc
     2101 cggatcgaca taccggatct gccgcaggca aacggtgatc gcg
//
LOCUS       DASYJA010000040         4509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_188395, whole genome shotgun sequence.
ACCESSION   DASYJA010000040 DASYJA010000000
VERSION     DASYJA010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188395"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VGO02_00845"
     CDS             complement(<1..626)
                     /locus_tag="VGO02_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEV7476265.1"
                     /translation="MSDEFRPPVRFPHLLAKVNAFLLVALLLVATFVGIVAHKQGWFV
                     HQSTIRVITPDALGITKGMPVKLYGFTIGSVKEMQLVTGGVDVQLEITSEHMARIPKG
                     SRARFARESGVIGASVIEIIPGKPIAGPLTEGEQIEFDRSRGISEIVEEFRRQAAPAF
                     AELKQALSQVNRSGEDVAVILAALRAEAEQLPATHKAMRRMIENADRAT"
     gene            complement(623..1234)
                     /locus_tag="VGO02_00850"
     CDS             complement(623..1234)
                     /locus_tag="VGO02_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476266.1"
                     /translation="MSALALRYDLRRDGRPPVFVEVPGGATQRVRLPDSEAKARLVGA
                     VLSTRCSAGEELELFGETVRALRRTERARLHDRIGVLTQAMGLISNLNAWENISLQAA
                     YHGTHPVARIAKTARDALAALGTDPQRFLARLPDEMDAFERKLVAFVRLLAKEPALAV
                     IDGLGEGLNREQSARAARFEAEYRARQPEGTLLFVDLAEEGIQ"
     gene            complement(1231..2061)
                     /locus_tag="VGO02_00855"
     CDS             complement(1231..2061)
                     /locus_tag="VGO02_00855"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7476267.1"
                     /translation="MERRVRGLVQNSGERENRVIRALREVEGGLRLLVNVWLRMPALR
                     RAPVRESFLRQVYFTGVTASTGVVLRASGLGALIIAFTMQVLDADTSLAVKILLWVVL
                     REVGPLAAAMVVILRTGTAMSAELAMMRISGQTEALRRMGIEAYDYLVVPRVAGVMIA
                     TAALTCYIQFLAVAGGMILSPFVIQASFTELYTQFFDLVAPVDFAYSILKSLMFGFAI
                     ALTCCYHGLNPPAFTQNGVPQAVTRAVTQSAVAVLTINALFAYLVFGVLFFGLVRAVA
                     "
     gene            2118..2369
                     /gene="rpsP"
                     /locus_tag="VGO02_00860"
     CDS             2118..2369
                     /gene="rpsP"
                     /locus_tag="VGO02_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEV7476268.1"
                     /translation="MVVIRLARGGANKRPFYHVVVADSRRAVQGKFLERLGFYDPKAP
                     AGRESLRVNLERVKHWTGKGAQLSPTAKRLVKLFAKKAA"
     gene            2383..2841
                     /gene="rimM"
                     /locus_tag="VGO02_00865"
     CDS             2383..2841
                     /gene="rimM"
                     /locus_tag="VGO02_00865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEV7476269.1"
                     /translation="MGRVAGHYGVRGWIRVQRPEAALADCGRWWIGGQEYPVEETKQH
                     SGALLAKLTGIDSREAALKLKGSEVAVRRESLPKPDEGKYYLTDLVGLEVVNEQGQAL
                     GVVRRIFTNGAQEVMEVVGDRSRLLPWVPAIVKGVDLEKKQIDVEWGADW"
     gene            2841..3581
                     /gene="trmD"
                     /locus_tag="VGO02_00870"
     CDS             2841..3581
                     /gene="trmD"
                     /locus_tag="VGO02_00870"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEV7476270.1"
                     /translation="MRFDVVTLFPEMFSAVTHSGITSRALDAGLWALTTWNPRDFTTD
                     NYRTVDDRPYGGGPGMVMLAEPIERALDAVRSSGGGRVVYLTPQGRKLDHRKVMEFSK
                     ESCLTLLCGRYEGIDERLLARRVEEEVSVGDFVLSGGELAAMALMDAVVRQLPGALGD
                     ENSALEESFADGLLDCPQYTRPEVYAGDGTPQVLMSGHHENIRRWRLKQALGRTWLRR
                     PDLLAVKTLNEEQKKLLAEFQQEHRSRA"
     gene            3578..3964
                     /gene="rplS"
                     /locus_tag="VGO02_00875"
     CDS             3578..3964
                     /gene="rplS"
                     /locus_tag="VGO02_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEV7476271.1"
                     /translation="MNLIQKLEQEEIQRLKKNVPDFGPGDTVVVNVNVVEGERKRSQA
                     FEGMVIAKKNRGLNSSFIVRKISSGEGVERTFQTFSPLIASIEVKRKGDVRRAKLYYL
                     RARSGKSARIRERTGAAEDGAGAAEE"
     gene            3974..>4509
                     /gene="ribB"
                     /locus_tag="VGO02_00880"
     CDS             3974..>4509
                     /gene="ribB"
                     /locus_tag="VGO02_00880"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEV7476272.1"
                     /translation="MRRETAAISPVTEIIAAIRGGEIVVLVDDEDRENEGDLVFAAEF
                     VTAEKINFLAKHGRGLICMPITEAHAERLGLKPMVEQNRSRHGTNFTVSIEAAEGIAT
                     GISAHDRALTVKIASSVNAKPEDIVQPGHVFPLIAQQGGVLVRAGHTEACCDLARLAG
                     LAPAAVLCEIMRDDGTMAR"
BASE COUNT          785 a         1437 c         1603 g          684 t
ORIGIN      
        1 gtggcgcggt cggcgttttc gatcatccgg cgcatcgcct tgtgggtcgc cggcagctgc
       61 tcggcctcgg cgcgcagcgc ggcgaggatc accgccacgt cctcgcccga gcggttgacc
      121 tgggacaggg cctgcttcag ctcggcgaag gcgggcgccg cctggcggcg gaactcctcg
      181 acgatctcgg agatgccgcg gctgcggtcg aactcgatct gctcgccctc ggtgagcggg
      241 ccggcgatcg gcttgccggg aatgatctcg atcacgctcg cgccgatcac gcccgattcg
      301 cgggcaaatc gggcgcgaga acccttgggg atgcgcgcca tatgctcgct cgtgatttcg
      361 agctgcacgt cgacgccgcc ggtgaccagc tgcatctcct tcaccgagcc gatggtgaag
      421 ccgtacagct tgaccggcat gcccttggtg atgccgagcg cgtccggggt gatgacacgg
      481 atggtgctct ggtgcacgaa ccagccctgc ttgtgcgcga cgatgccgac gaaggtcgcc
      541 accagcagca gcgccaccag caggaacgcg ttcactttgg cgagcaggtg cggaaacctg
      601 accggcggcc ggaattcgtc gctcattgga tcccctcctc cgccaggtcc acgaacagca
      661 gcgtgccctc aggctggcgg gcacgatatt ctgcctcgaa gcgcgccgcg cgggcgctct
      721 gctcgcggtt caggccctcg cccaggccgt cgatcaccgc cagcgccggc tccttggcga
      781 gcaggcgcac gaaggccacc agctttcgct cgaaggcgtc catctcgtcg ggcaggcggg
      841 cgaggaagcg ctgcgggtcg gtgccgagcg cggcgagcgc gtcgcgcgcg gtcttggcga
      901 tgcgtgccac cggatgagtg ccgtgatagg cggcctgcag cgaaatgttc tcccacgcgt
      961 tcagattgga gatcagcccc attgcctggg tcagcacccc gatccggtcg tgcagccgcg
     1021 cccgctcggt gcgcctgagg gccctcaccg tctcgccgaa cagctcgagc tcctcgcccg
     1081 cgctgcagcg cgtgctcagc accgcgccga cgagccgcgc cttggcttcg ctgtccggca
     1141 ggcgcacgcg ctgcgtggct ccgccgggca cttcgacgaa taccggcggg cgaccgtcgc
     1201 gcctgaggtc gtagcgcagg gcgagcgcgc tcacgcgacc gccctgacca gcccgaagaa
     1261 cagaacgccg aacaccaggt aggcgaacag ggcattgatg gtgagcaccg cgaccgcgct
     1321 ctgcgtgacc gcgcgggtga ccgcctgcgg caccccgttt tgggtgaagg ccggcgggtt
     1381 gaggccgtgg tagcagcagg tcagtgcgat ggcgaagccg aacatcaggc tcttcaggat
     1441 cgagtaggcg aagtcgaccg gcgccaccag gtcaaagaac tgcgtgtaga gctcggtgaa
     1501 cgaggcctgg atcacgaacg ggctgaggat catgccgccc gccacggcca ggaactggat
     1561 gtagcaggtg agggctgcgg tggcgatcat caccccggcg acgcgcggca cgaccaggta
     1621 gtcgtaggcc tcgatgccca tgcggcgcag cgcctcggtc tgcccgctga tgcgcatcat
     1681 cgcgagctcg gccgacatcg cggtgccggt gcgcaggatc acgaccatcg ctgcggcgag
     1741 cggaccgact tcgcgcagca ccacccacag caggatcttg accgcgaggc tagtgtccgc
     1801 gtcgagcacc tgcatggtga acgcgatgat cagggcgccc agccccgagg cgcgcagcac
     1861 caccccggtc gaggccgtga ccccggtgaa gtagacctgg cgcaagaagg actcgcgcac
     1921 cggggcgcgg cgcagcgccg gcatgcgcag ccagacgttc accaacagcc gcagcccgcc
     1981 ttcgacctcg cgcagcgccc ggatcacgcg gttctccctc tcgccgctat tctgcaccaa
     2041 gccgcgcaca cgccgttcca aaagaccgat ttttccctta taatccgcgc cttttcgcgg
     2101 ttgacgggga ttcccaaatg gtagtgattc gactggcgcg cggcggcgcc aacaagcggc
     2161 ccttttatca cgtggtggtg gccgattcgc gccgtgcagt gcagggcaag ttcctcgagc
     2221 ggctgggctt ctatgatccg aaggcgcccg cggggcgcga gtctctgcgc gtcaacctgg
     2281 agcgcgtcaa gcactggacg ggcaagggcg cgcagctctc gcccacggcg aagcggcttg
     2341 tgaagctgtt tgccaagaaa gccgcctgag agcctggtag agatgggccg cgtcgccggt
     2401 cactacggcg tgcgcggctg gatccgggta cagcggcccg aggcggcgct ggcggactgc
     2461 ggcaggtggt ggatcggcgg gcaggagtat cccgtcgaag agacgaaaca gcattcgggc
     2521 gcgctgctcg cgaagctgac cgggatcgac agccgagagg cggcgctgaa gctgaaggga
     2581 tcggaagtgg cggtgcggcg cgagtcgctg cccaagcccg atgagggaaa gtactacctc
     2641 accgacctgg tgggcctgga ggtggtgaac gagcagggac aggcgcttgg cgtggtgcga
     2701 cggatcttca cgaacggcgc gcaggaagtg atggaggtgg tcggggaccg gtcgagattg
     2761 ctgccctggg tcccggcgat cgtaaaagga gtcgatctgg agaagaaaca gatcgacgtc
     2821 gagtggggag cggactggtg atccgcttcg acgtggtgac gctgtttccg gagatgtttt
     2881 cggcagtcac gcacagcggc atcaccagcc gcgcgctgga cgcgggactg tgggcgttga
     2941 cgacgtggaa tccgcgcgat ttcacgaccg acaactaccg gacggtggac gaccgtccct
     3001 acggcggcgg gccgggcatg gtgatgctcg ccgagccgat cgagagggcg ctggacgcgg
     3061 tcaggtccag cggcggcggc cgggtggtgt acctgacgcc gcagggcagg aagctggacc
     3121 accgcaaggt gatggagttt tcgaaggaaa gctgcctgac cttgctgtgc ggcaggtatg
     3181 aagggataga cgagcgcctt ctggcgcggc gcgtggagga agaggtctcg gtcggagact
     3241 tcgtcctgtc gggcggcgag ctggcggcga tggcgctgat ggacgcggtg gtgcggcagc
     3301 tgccgggagc gctcggcgac gagaattcgg cgctggaaga gtcgtttgcc gacggcttgc
     3361 tggactgccc tcagtacacg cggcctgagg tgtacgcggg agatggaacg ccacaggtgc
     3421 tgatgtccgg gcaccacgag aacatccggc gctggcggtt gaagcaggcg ctggggcgga
     3481 cctggctgcg caggcccgac ctgctggcgg tgaagacgct gaacgaagag caaaagaagc
     3541 tgctggcgga attccagcag gagcacagga gccgagcatg aatctgatcc agaaactcga
     3601 gcaggaagag atccagcgcc tgaagaagaa cgtgcccgac ttcgggccgg gcgacacggt
     3661 ggtggtcaac gtcaacgtgg tcgagggcga gcgcaagcgc tcgcaggcct tcgagggcat
     3721 ggtgatcgcg aagaagaacc gcggcctcaa ttcctccttc atcgtgcgca agatctcctc
     3781 gggcgagggt gtcgagcgta cgttccagac cttctcgccg ctcatcgcct cgatcgaggt
     3841 gaagaggaag ggcgacgtgc gccgcgccaa gctgtactac ctgcgcgcgc gctcgggcaa
     3901 gtcggcgcgc atccgcgagc gcaccggcgc ggccgaagac ggagcaggcg ccgccgagga
     3961 gtaaaatcgg ctgatgcgcc gcgaaaccgc cgccatcagc ccggtcaccg agatcatcgc
     4021 cgccatccgc ggcggcgaga tcgtcgtcct ggtggacgac gaggaccgcg agaacgaagg
     4081 cgacctggta ttcgccgccg agttcgtcac cgccgagaag atcaatttcc tcgccaagca
     4141 cgggcgcggt ctgatctgca tgccgatcac cgaggcgcac gccgagcgcc tcggcctcaa
     4201 gccgatggtc gagcagaacc gctcgcgcca cggcaccaat ttcaccgtct cgatcgaggc
     4261 cgccgagggc atcgccaccg gcatttcggc ccatgaccgc gccctgaccg taaaaatagc
     4321 ttcttctgtg aacgcaaaac cggaagatat tgtgcagccc ggacatgtct ttccgctgat
     4381 cgcgcagcag ggcggcgtgc tggtgcgcgc cgggcatacc gaggcgtgct gcgacctcgc
     4441 ccggctcgcc gggctcgcgc cggccgcggt gctatgcgag atcatgcgcg acgacggcac
     4501 catggcgcg
//
LOCUS       DASYJA010000041         1597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1369431, whole genome shotgun sequence.
ACCESSION   DASYJA010000041 DASYJA010000000
VERSION     DASYJA010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369431"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VGO02_00885"
     CDS             complement(<1..536)
                     /locus_tag="VGO02_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEV7476273.1"
                     /translation="MDHKSIDRTSAELSGFLQPLARLKQALAKDEFSLYCQPIRSLQD
                     GRYAMGEVLVRMREEEAALLPPGDFFPVFEHYRMMPQLDRWVLRHTIKRLKAGSRIPQ
                     FTVNLAAQTIEDLEFPKFVAAELMGSGIAASGVAFEIDENDALSRPEACARFASAVKP
                     IGCGVLLDGFGRKSVTFEP"
     gene            553..1332
                     /locus_tag="VGO02_00890"
     CDS             553..1332
                     /locus_tag="VGO02_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7476274.1"
                     /translation="MPQPFFRVDRLTKRFGGLTAVNNVSFELRRDEIVGLIGPNGAGK
                     TTLLRLITKILRPDAGSIVFNGEDITALRPWDVVERGIAGTFQNTRPFRHLPIVANVM
                     VPLVAPRARRNGEWVNSVEAKAMDALEFVCISDMALEPASALSQGDLKRLEVARAIAT
                     EPELLLLDEPLGGLNPAESELLAKSVRRLHKGGRFGRLHSEGPAVLMIEHKLKELMAI
                     VDRVIVVDYGEIIADGPPAEVVKNPKVIEAYLGSGSEYAAA"
     gene            1319..>1597
                     /locus_tag="VGO02_00895"
     CDS             1319..>1597
                     /locus_tag="VGO02_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7476275.1"
                     /translation="MPLLEVKNLSVRYDKAQILNGVSLAVEAGEFVGLVGPNGAGKST
                     LLRAATGMLATTRGRIQLGDRDLTGASIESIARTGVAHVPEGRRLFAGL"
BASE COUNT          262 a          542 c          547 g          246 t
ORIGIN      
        1 ggctcgaagg tcaccgactt gcggccgaag ccatccagca gcacgccgca gccgatcggc
       61 ttcaccgcgc tggcgaagcg agcgcaggct tccggccgtg agagcgcgtc gttctcgtcg
      121 atctcgaagg caacgcccga cgccgctatg cccgagccca tcagctccgc ggcgacgaac
      181 ttcgggaact caaggtcctc gatcgtctga gccgccaggt tgaccgtgaa ctgcgggatg
      241 cgcgagcctg ccttcaggcg cttgatggtg tgccgcagca cccagcggtc gagctgcggc
      301 atcatccggt agtgctcgaa caccgggaag aaatcccccg gcggcaggag cgcagcttct
      361 tcctcgcgca tgcgcaccag cacctcgccc atcgcgtagc gcccgtcctg gagcgaccgg
      421 atcggctggc aatacaagct gaactcgtcc tttgccagcg cctgcttcag gcgagcgagc
      481 ggttgcagaa agccgctcag ttccgcgctg gtccggtcga tcgacttatg gtccacaggg
      541 tattctagcg gcatgccgca accgttcttc cgggtggacc gcctcaccaa gcgcttcggc
      601 gggctgaccg cggtcaacaa cgtgagcttc gagctgcggc gcgacgagat cgtcggcctg
      661 atcgggccga acggcgccgg caagaccacg ctgctgcgcc tgatcaccaa gatcctgcgt
      721 cccgacgccg gcagcatcgt cttcaacggc gaggacatca ccgcgctgcg tccctgggac
      781 gtcgtcgagc gcggcatcgc cggcacgttc cagaacacgc ggccgttccg ccacctgccg
      841 atcgtcgcca acgtcatggt gccgctggtg gcgccgcgcg cgcggcgcaa cggcgagtgg
      901 gtaaatagcg tcgaggcgaa ggcgatggac gcgctcgagt tcgtctgcat ctccgacatg
      961 gcgctcgagc cggcctcggc gctgtcgcag ggcgacctga agcgcctcga ggtggcgcgc
     1021 gccatcgcca ccgagccgga gctgctgctg ctcgacgagc cgctcggcgg cctgaacccg
     1081 gcggagtccg agctgctggc gaaatcggtg cggcgcctgc acaagggcgg ccgcttcggc
     1141 cgcctgcact cggagggccc ggcggtgctg atgatcgagc acaagctgaa ggagctgatg
     1201 gcgatcgtcg accgcgtgat cgtcgtcgac tacggcgaga tcatcgccga cgggccgccg
     1261 gcggaggtgg tcaagaatcc gaaggtgatc gaggcgtacc tcggcagcgg gagcgaatat
     1321 gccgctgctt gaggtgaaga acctcagcgt gcgctacgac aaggcgcaga tcctgaacgg
     1381 cgtttcgctc gcggtcgaag cgggcgaatt cgtcggcctg gtgggcccga acggcgccgg
     1441 caagtcgacg ctgctgcgcg cggcgaccgg catgctcgcg acgactcgtg gccgcattca
     1501 gctcggcgac cgcgacttga ccggcgcctc gatcgaaagc atcgcgcgca ccggcgtcgc
     1561 gcacgttccc gaagggcggc gcttgttcgc cggcttg
//
LOCUS       DASYJA010000042         5677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3185132, whole genome shotgun sequence.
ACCESSION   DASYJA010000042 DASYJA010000000
VERSION     DASYJA010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5677
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3185132"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VGO02_00900"
     CDS             <1..583
                     /locus_tag="VGO02_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV7476276.1"
                     /translation="GVSALITGLQPVFTAVLGGWLLHERTTRRQWVGLALGLLGVVLV
                     VGDRIAVQHLTVLAVLLSALALASITLGTVWQKRHGGAIDLRTGAAIQFAAAALVLAP
                     FAFLLETRQVRWSGEFVFALAWLVLVLSFGAIFLLLYLIRHGAATRVASLFYLVPPTT
                     ALMAWPLFGESYSALAAAGMGLGMLAVWLVTKP"
     gene            562..1791
                     /locus_tag="VGO02_00905"
     CDS             562..1791
                     /locus_tag="VGO02_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV7476277.1"
                     /translation="MAGDQTVTLQYRPNDPAVLADPFALYRRLRDEDPAHWSPLLKAW
                     VLTRYEEVRRVCLDTRMSSDRLRPFFATLPSAEAARIGELIRYLTLWMVFRDPPEHTR
                     LRRLASKVFNVRSMHALRPNVEALTAWLLDQIGGREEFDFIAEFAGPLPALVIMDMLG
                     APRGELARLKRLSDEMALFIGSARDSPEKYARAESATHEMAGLFRELIAARRAAPQRD
                     LLSELVALEDAGDRLSEDELVATCILLLFAGHETTTHHLANGLATLLRFPRELERLRG
                     EPSLAPAAVEELLRYDGPIGAQVRIVQEAQDFHGRTFRAGDRVFLLMNAANRDERAYP
                     DPDRLDLGRSGVPHLTFGFGMHICLGFPLARLEVQIALPAVLKRWRRIEPAGGKPEWI
                     DSMVIRGMQAMPLRVRG"
     gene            1788..2666
                     /locus_tag="VGO02_00910"
     CDS             1788..2666
                     /locus_tag="VGO02_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV7476278.1"
                     /translation="MSAEPDHLPQRLWWLLAALTLAWGFNWTAMKVALAGVPPWTFRT
                     LCLGLGSAVLFLVLRAGGHALTVPPGQRVRLAVLAFFNITCWNLLVAFGMTMIPSGRA
                     AILAYTMPVWAVPLSIWVLGERLTSRKLLGLVLGMAGLALLVGDVFASPGTLLVLGAA
                     ISWAIGTVLQKRYPMAMPNGAYTAWIMLLGGVPIFAGALLFEDLGALREIGLWPALAV
                     AYNVIVAFAFAHWAWIKIATSVPVSVFSISMLIIPVVGVLSGMLFLGERPSWSEYAAL
                     LLILCALATVIRPQAR"
     gene            complement(2654..3718)
                     /locus_tag="VGO02_00915"
     CDS             complement(2654..3718)
                     /locus_tag="VGO02_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEV7476279.1"
                     /translation="MKITQVAAVPLSIPFQHGLGSGRRLDFCLVRVETDGGIVGWGDA
                     FAYNCRTTVAAAVNDMIAPLALGHDAADIAGLHHSIQKRLHIFGRFGVQAYALSGLDI
                     ALWDIAGKAAGEPVHRLLGGTKRASVPCYASLLRYGDAAMVAKHCERALGEGYTSIKL
                     HEVTDAAVAAGRAAVPRQVPLTLDVNCEWPPQDAIEAALRFKPHGLQWLEEPAFPPED
                     FAALRAVGEASGIPIAAGENLCFATQFKAAFDAGALAYAQPSVTKVGGITEFVKVVQL
                     ATDRGVQVAPHSPYFGPGALATLHLLAALVPEARFEYYYLWPDASLYPGLFGGSSVEV
                     PRAAGLGLDPDPGVMQRYRA"
     gene            complement(3715..4560)
                     /gene="gluQRS"
                     /locus_tag="VGO02_00920"
     CDS             complement(3715..4560)
                     /gene="gluQRS"
                     /locus_tag="VGO02_00920"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEV7476280.1"
                     /translation="MYVGRFAPSPTGPLHFGSLVAALASYMEARHAKGKWLVRMEDLD
                     KPREQRGAADAILRTLDGFGLHWDGAVEYQSRRVALYRDALDRLKPHTYPCGCTRSEI
                     ADSALALHGAPVYGGTCRNGLPPGKTARSVRLKVEGEIRFVDRAQGPQVQRLERDVGD
                     FVLRRADGQFAYQLAVVVDDARQGITDVVRGADLLDSTARQIYLQQRLGHSVPRYLHV
                     PVAVNAAGEKLSKQTGAEPVDALGRGEALRRAFAFLGQAPTDDLDEAVRRWDASRIPS
                     RRIAS"
     gene            4605..4979
                     /gene="trxA"
                     /locus_tag="VGO02_00925"
     CDS             4605..4979
                     /gene="trxA"
                     /locus_tag="VGO02_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV7476281.1"
                     /translation="MATVELTQDNFEKTVNDNPMVIVDFWAPWCGPCKGFAPVYEKAA
                     EGHPDIVFAKVNTDEQQELAGAFGIRSIPTLMVFREKVILFQQAGALPGNALEQVITQ
                     AKSLDMAKVRQEVNAKNGTDPA"
     gene            4960..5658
                     /locus_tag="VGO02_00930"
     CDS             4960..5658
                     /locus_tag="VGO02_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEV7476282.1"
                     /translation="MEQIRRSGERGHANHGWLDSYHSFSFADYHDPQHMGYGPLRVIN
                     EDRVQPGQGFGTHGHRDMEIISYVLEGALAHKDSMGTGSTIVPGDVQRMSAGNGVRHS
                     EFNQEKAKVTHFLQIWIEPSVSGIAPSYEQKHFSAAEKRGRLRLIASPDGADGSVSIH
                     QDARVHAGLFDGAERASYSLPQGRLGYVHVARGSVQANGKKLAAGDALKTDAAEIRIE
                     GGEQAEILLFDLPQ"
BASE COUNT          862 a         1953 c         1941 g          921 t
ORIGIN      
        1 gggggtgtcg gccctgatca caggcctgca gccggtgttc acggcagtcc ttggaggctg
       61 gctgctacat gaacggacta cccggcggca atgggtcggc ctggcgctcg ggctgctagg
      121 cgtcgtcctc gtcgtaggcg accggatcgc cgtgcagcac ctgaccgtgc tcgcggttct
      181 cctctcagcc ctggcgctcg ccagcatcac gctgggcacg gtctggcaga agcggcacgg
      241 cggcgcgatc gacctgcgca ccggtgccgc catccagttc gccgcggcgg cgctcgtgct
      301 ggcgccgttc gccttcctgc tggagacgcg gcaagtgcgc tggagcggag agttcgtttt
      361 tgccctcgcg tggctggtgc tggtgctttc gtttggcgct atcttcctgc tcctctacct
      421 gatccggcac ggcgccgcga cgcgcgtggc gagcctgttc tacctggtgc cgccgacgac
      481 cgcgctgatg gcctggccgc tgttcggcga gtcctacagc gcgctcgccg cggccgggat
      541 gggactcggt atgctcgcgg tgtggctggt gaccaaaccg tgacgctgca gtaccgcccg
      601 aacgacccgg cagtcctggc cgaccccttt gcgctgtatc ggcggctgcg cgacgaggac
      661 ccggcgcact ggagcccgct gctcaaggca tgggtgctga cgcgctacga ggaggtgagg
      721 cgggtatgcc tggatacgcg catgtcgtcc gaccggctgc gcccgttctt cgccacgctg
      781 ccgtcggcgg aagcggcgcg gatcggcgag ctgatccgct acctcacgct ctggatggtg
      841 ttccgcgacc cgcccgagca tacgcggctg cggcgcctcg cttccaaggt gttcaacgtg
      901 cgctcgatgc acgcgctgcg cccgaacgtg gaggcgctca ccgcctggct gctcgaccag
      961 atcggcggcc gcgaggaatt cgacttcatc gccgagttcg caggtcctct gccggcgctg
     1021 gtcatcatgg acatgctcgg cgcgccgcgc ggcgagctcg cgcggctcaa gcgcctgtcg
     1081 gacgagatgg cgctgttcat cggcagcgcg cgcgactcgc cggagaaata cgcccgcgcc
     1141 gagtcggcga cgcatgagat ggccgggctg ttccgtgaat tgatcgcggc gcgccgcgcc
     1201 gcgccccagc gcgacctcct gagcgagctg gtcgcgctcg aggacgcggg cgaccggctg
     1261 tccgaggacg agctggtggc gacctgcatc ctgctgctct ttgccgggca cgaaaccacc
     1321 acgcaccacc tcgccaacgg tctcgccacg ctcctgcgct tcccgcgcga gctcgagcgg
     1381 ctgcgcggcg agccgtcgct cgcgccggcc gcggtcgagg agctgctgcg ctacgacggc
     1441 cccatcggcg cccaagtgcg catcgtgcag gaagcgcagg acttccacgg caggacattc
     1501 cgggcgggcg accgggtatt cctgctgatg aacgcggcca accgcgacga gcgcgcctac
     1561 cccgaccccg accggctcga cttgggccgc agcggcgtac cgcatctgac gttcggcttc
     1621 ggcatgcata tctgcctcgg cttcccgctc gcgcggctcg aggtccagat cgccctgccg
     1681 gcggtgctca agcgctggcg gcggatcgag ccggcaggcg gcaagcccga gtggatcgac
     1741 tccatggtga tccgcggcat gcaggcgatg ccgctacgcg tgcgcggttg agcgccgagc
     1801 ccgatcacct gccgcagcgc ctgtggtggc tgctggcggc cctcacgctc gcctggggct
     1861 ttaactggac ggccatgaag gtggcgcttg ccggcgtgcc gccctggacg ttccgcacgc
     1921 tatgcctcgg actgggttcc gcggtgctgt tcctggtgct cagggcaggc ggacacgcgc
     1981 ttaccgtgcc gccgggccag cgcgtacgcc tggcggtgct tgccttcttc aacatcactt
     2041 gctggaacct gctcgtcgcc ttcggcatga cgatgatccc ttccggccgc gcggcgatcc
     2101 ttgcctacac catgccggtg tgggcggtgc cgctctccat ctgggtgctc ggtgagcggc
     2161 tgacgagccg caagctcctc ggactcgtgc tcggcatggc cggcctcgcg cttctggtgg
     2221 gcgacgtgtt cgcgagcccg ggcacgctgc tggtcctggg cgccgcgatt tcctgggcga
     2281 tcggcaccgt gctgcaaaag cgctacccga tggcgatgcc gaacggggcc tacacggcct
     2341 ggatcatgct gctcggcggc gtgccgattt tcgccggcgc gctgctgttc gaggatctgg
     2401 gcgcgctgcg cgagatcggc ctgtggccgg cgctcgccgt cgcctacaac gtgatcgtcg
     2461 ccttcgcctt cgcgcactgg gcctggatca agatcgccac ctcggtgccg gtgagcgtgt
     2521 tctcgatcag catgctgatc atcccggtgg tcggcgtgct gagcggcatg ctgttcctcg
     2581 gcgagcggcc gagctggagc gagtacgccg ctctgctgct cattctctgc gccctggcga
     2641 cggtgatccg tcctcaggcg cgatagcgct gcatgacccc cggatcggga tccagtccga
     2701 gcccggcggc tcgcggcacc tccaccgagc tgccgccgaa cagtcccgga tagagcgatg
     2761 cgtcgggcca caggtagtag tactcgaagc gtgcttccgg gaccagcgct gcgagcagat
     2821 ggagcgtggc gagcgcgcca gggccgaagt agggcgaatg cggcgcgacc tggacgccgc
     2881 gatcggtagc gagctgcacc actttcacga actcggtgat gccgccgact ttggtcaccg
     2941 agggctgcgc gtaggcgagg gccccggcgt cgaaggcggc cttgaactgg gtcgcgaagc
     3001 acaggttctc gccggcggcg atcggaatgc cgctcgcctc gcccacggcg cgcagggccg
     3061 cgaagtcctc cggcggaaac gccggttcct cgagccattg caatccgtgc ggcttgaagc
     3121 gcagcgcggc ctcgatagcg tcctgcggcg gccattcgca gttcacgtcg agggtcagcg
     3181 ggacctgcct gggcacggcg gcgcgccccg cggcgacggc cgcatcggtc acctcgtgaa
     3241 gcttgatcga ggtgtagcct tcgcctagcg cgcgctcgca gtgtttggcc accattgccg
     3301 catcgccgta acgcagcagg ctcgcataac acgggaccga cgcgcgcttc gtcccgccga
     3361 gaaggcggtg gaccggctcg ccggctgcct tgccggcgat gtcccacaac gcgatgtcga
     3421 gccctgacaa cgcataagcc tggacgccga aacgcccgaa gatatgcagc ctcttctgga
     3481 tcgagtggtg caagcccgca atatcggcgg catcgtgtcc gagcgcgagg ggcgcaatca
     3541 tgtcgttcac ggcggcggct accgtggtgc ggcagttgta cgcgaacgca tccccccagc
     3601 cgacgatgcc gccgtccgtc tcgacgcgta ccaggcagaa atcgagcctg cgcccgctgc
     3661 ccaggccgtg ctggaaggga atcgacaacg gcaccgcagc cacctgcgtg atcttcatga
     3721 cgcgatcctg cgcgacggga tgcgcgaggc gtcccaccgt cgcactgcct cgtcgagatc
     3781 gtcggtcggg gcttggccga gaaaggcgaa ggcccggcgc agcgcttcgc cgcggccgag
     3841 ggcgtcgacc ggttcggcgc ccgtctgctt ggaaagcttc tcgcccgcgg cgttcacggc
     3901 caccggcaca tgcaggtagc gcggcacgga atggccgagc cgctgctgca ggtagatctg
     3961 ccgcgcggtc gagtcgagca ggtcggcgcc gcggaccacg tcggtgatgc cctgccgggc
     4021 atcgtcgact acaacggcga gctggtaggc gaactgcccg tcggcacgcc gcagcacgaa
     4081 gtcgccgacg tcgcgctcga gccgctgcac ctgcggaccc tgggcccggt cgacgaagcg
     4141 gatttcgcct tcgaccttga gcctgactga gcgtgcggtc tttcccggcg gcaggccgtt
     4201 gcgacacgtg ccgccgtaca cgggggcgcc gtgcagcgcc agcgccgagt cggctatctc
     4261 gctgcgcgtg cagccgcagg gataggtatg cggcttcagg cggtcgagcg cgtcgcgata
     4321 aagcgcaacg cgcctgctct ggtactcgac ggcaccatcc cagtgcaaac cgaacccgtc
     4381 gagcgtacgc aggattgcgt ctgccgcgcc gcgctgctcg cgcggcttgt cgaggtcttc
     4441 catgcgcacc agccacttgc cttttgcatg cctcgcctcc atgtaactcg ccagcgcagc
     4501 gaccagcgag ccgaagtgca gcggccccgt cggcgacggg gcgaaccggc ctacgtacat
     4561 ggttgtctag agtacactgc gcagaaattc acgaaaggaa cctcgtggca accgtagagc
     4621 tcacccaaga caatttcgag aagaccgtca acgacaaccc gatggtcatc gtggacttct
     4681 gggcgccgtg gtgcggcccc tgcaagggtt ttgccccggt ctacgagaag gccgctgaag
     4741 ggcatcccga catcgtcttc gccaaggtca acaccgacga gcagcaggag ctggccggcg
     4801 ccttcggcat ccgctcgatc ccgaccctga tggtgttccg cgagaaagtg atcctgttcc
     4861 agcaggccgg cgcgctgccc ggcaatgccc tcgagcaggt catcacgcag gcgaagagcc
     4921 tcgacatggc caaggtccgc caggaagtga acgccaagaa tggaacagat ccggcgtagc
     4981 ggcgagcgcg gccacgccaa ccacggctgg ctcgactcgt accacagctt ctcgttcgcg
     5041 gactaccacg acccgcagca catgggctac ggccccctgc gtgtcatcaa cgaggaccgc
     5101 gtgcagcccg ggcagggttt cggaacccat ggtcaccgcg acatggagat catcagctat
     5161 gtgctggagg gcgcgctcgc gcataaggac tcgatgggca ccggttcgac catcgtcccc
     5221 ggcgacgtgc agcgcatgag cgcgggtaac ggcgtgcggc acagcgagtt caaccaagaa
     5281 aaagcgaagg tgacgcactt cctgcagatc tggatcgagc cgtcggtaag cggcattgcg
     5341 ccgtcgtacg agcagaagca tttcagcgcc gccgagaaac gcggccggct gcgcctcatc
     5401 gcctcgcccg acggcgcgga cggctcggtc agcatccacc aggacgcgcg cgtccacgcc
     5461 ggcctgttcg acggcgccga gcgcgctagc tactcgctgc cgcaaggccg cctcggctac
     5521 gtgcacgtgg cgcgcggcag cgtgcaggcg aacggcaaga agctcgcagc aggcgacgcg
     5581 ctgaagaccg atgcggccga gatccgcatc gaaggcggcg agcaggctga aatcctgctc
     5641 ttcgacttgc cgcaatagca cgccgcgatg agcggca
//
LOCUS       DASYJA010000043         2270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2095599, whole genome shotgun sequence.
ACCESSION   DASYJA010000043 DASYJA010000000
VERSION     DASYJA010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2095599"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VGO02_00935"
     CDS             <1..220
                     /locus_tag="VGO02_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEV7476283.1"
                     /translation="HLAKEKETGRFCHGDAPTLADICLAGQVIGAVGYFKCDVSGVPS
                     VMRIYEECMKIDAFSAAHPLKQPGAQAH"
     gene            224..955
                     /locus_tag="VGO02_00940"
     CDS             224..955
                     /locus_tag="VGO02_00940"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV7476284.1"
                     /translation="MKSVEIVDRSSLLAGLYKELNLERKKTAIVAIDMHRGHLDMEVA
                     TMPAKPEDARRVIANAKAVLDFSRAAGIPVIHVVLVYRRLKNIGSEGMTSPFWKAMHA
                     ALGETDRLTPGRKSTVREHNIEGSPGTQIIPELYQPSDYVINNKKRLDCFYGTDLRSL
                     LDTLQITNVVLMGINTNTCVLNTSFTAFNFDYRVVVLSDCVASMYGDDLHALGLQNVA
                     RCLGWVITNEQFFEKLKEERHELQP"
     gene            939..2180
                     /locus_tag="VGO02_00945"
     CDS             939..2180
                     /locus_tag="VGO02_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7476285.1"
                     /translation="MSFNRRRFVQAAGAVTLGTAFPVKAQTGPIRLGLMTVKTGPLAS
                     GGIDMERALVQYLKEKNNTLAGRKVELFVGDSAGVPAQARTKIQELVERDRIHIMIGP
                     LAAAEALAADDYIRQAQLLTLSVAAAEDLTQRKPNPWFTRGTATSAQSAHPMADYAFK
                     EMKMRRMVTIADDFAYGHEMSAGFQRVFEELGGKIVQKMFPPLTVPDYGTYLAQLKTN
                     VDGIFLGFAGANGFRYLRQFNEYGLMKKVTPVGGMTALDEAVLRNMGDEALGIVTSCW
                     YSAEIENAVNKRFVANFRVDWKYDPGFYAAATYTEAAVLEAALNQIKGNIEDKPAFMK
                     AVRGLKNVETCRGPVSFDQYGNVVGNNYIRKVERKDGRLVNRVIKTYPNVSQFWTYKP
                     EDFLKNPVYSRDYPPAKNLES"
BASE COUNT          467 a          701 c          732 g          370 t
ORIGIN      
        1 ccacctggcg aaggagaagg agacgggccg cttctgccac ggcgatgcgc cgacgctcgc
       61 cgatatctgc ctcgccgggc aggtgatcgg cgccgtcggc tacttcaaat gcgacgtctc
      121 gggcgtgccg agcgtgatgc gcatctacga ggagtgcatg aagatcgacg ctttctccgc
      181 cgcgcacccg ctcaagcagc cgggcgcgca ggcgcattga gtcatgaaga gcgtcgagat
      241 cgtcgatcgg tccagcctgc tcgctgggtt gtacaaagaa ttgaatctgg agagaaaaaa
      301 aacagcgatc gtcgccatcg acatgcaccg cggccacctc gacatggagg tggcgaccat
      361 gccggcgaag ccggaggacg ccaggcgggt gatcgccaat gcgaaagcgg tcctggattt
      421 ttcgcgggca gccgggattc cggtcattca cgtagtgctg gtgtacagaa ggctgaaaaa
      481 catcggcagc gaggggatga cttcgccgtt ctggaaggcg atgcatgcgg cgctgggcga
      541 gaccgaccgc ctgactcccg gtcgcaagag cacggtgcgc gagcacaaca tcgaaggctc
      601 gcccgggacg cagatcatcc ccgagctgta ccagccgtcc gactacgtga tcaacaacaa
      661 gaagaggctg gactgcttct acggcacgga cctgagaagc ctcctcgata cgctgcagat
      721 cacgaacgtg gtgctgatgg gcatcaacac caatacttgc gtcctcaaca cgtcgttcac
      781 cgcattcaac ttcgactacc gcgtcgtggt gctgtcagac tgcgtcgcga gcatgtatgg
      841 ggacgacctg cacgcgcttg gcctgcagaa tgtcgcgcgc tgcctcggct gggtgatcac
      901 caatgagcag ttcttcgaga agctaaagga ggagagacat gagcttcaac cgtagacggt
      961 ttgtgcaggc ggccggtgca gtcacgctgg gaactgcttt tccagtaaaa gcgcaaactg
     1021 ggccgatccg gctgggcctg atgaccgtga agaccggccc gctcgcctcg ggcggcatcg
     1081 acatggagcg cgcgctggtg cagtacctga aggagaagaa caacacgctc gccgggcgca
     1141 aggtcgagct gttcgtcggc gacagcgccg gcgtgccggc gcaggcgcgc accaagatcc
     1201 aggagctggt cgagcgcgac aggatccaca tcatgatcgg gccgctcgcc gcggccgaag
     1261 cgctcgccgc cgacgactac atccgccagg cgcagctgct cacgctgtcg gtggcggccg
     1321 ccgaggacct gacgcagagg aagcccaacc cctggttcac gcgcggcacc gccacgtccg
     1381 cgcaatccgc gcatccgatg gccgactacg cgttcaagga aatgaagatg cggcgcatgg
     1441 tgaccatcgc cgacgacttc gcctacggcc acgagatgag cgccggcttc cagcgcgtgt
     1501 tcgaggagct gggcggcaag atcgtgcaga agatgttccc gccgctgacg gtgcccgact
     1561 acggcaccta tctcgcccag ctcaagacca acgtcgacgg catcttcctc ggcttcgccg
     1621 gcgccaacgg cttccgctac ctgcggcagt tcaacgagta cggcctgatg aagaaggtga
     1681 cgccggtcgg cggcatgacc gcgctcgacg aggcggtgct gcgcaacatg ggcgacgagg
     1741 cgctgggcat cgtcacctcg tgctggtatt cggcggagat cgagaacgcg gtcaacaagc
     1801 gcttcgtcgc caacttccgc gtcgactgga agtacgaccc gggcttctac gccgcggcta
     1861 cctataccga ggcggcggtg ctcgaggcgg cgctcaacca gatcaagggc aacatcgagg
     1921 acaagccggc gttcatgaaa gccgtgcgcg gcctgaagaa cgtggaaacc tgccgcggcc
     1981 cggtgtcgtt cgaccagtac ggcaacgtgg tcggcaacaa ctacatccgc aaggtcgagc
     2041 gcaaggacgg caggctggtg aaccgggtga tcaagaccta cccgaacgtc agccagttct
     2101 ggacctacaa gcctgaagac tttctgaaga acccggtgta ctcgcgggac tatcctccgg
     2161 ccaagaacct ggagtcataa gctgcaatat tgggcgatcc agacgctcaa tagcctggcg
     2221 ctgggaggat tgctgttcct cctggcggcc ggcttttcgc tgatcttcgg
//
LOCUS       DASYJA010000044         3394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1641352, whole genome shotgun sequence.
ACCESSION   DASYJA010000044 DASYJA010000000
VERSION     DASYJA010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641352"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VGO02_00950"
     CDS             complement(<1..621)
                     /locus_tag="VGO02_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476286.1"
                     /translation="MSELRNFVADLLERKGAAVDKAVDPDGLEVLAPAPVQKATGWPE
                     LTRLSFGRERSHGAIPIGLEGDWLDRFGALLEDEGRWSERAVSPAGAVPAPSDPGRLL
                     DRALDLPNAVWRFQGMTATWTRCLMLAFRYTAVSDEKREGLIWLGLNLGTGAVVSDIL
                     VRLRPALAQVLDWQAPDQATRLAAGSGWSAATLEARLRPLLDRQVRD"
     gene            complement(631..2937)
                     /locus_tag="VGO02_00955"
     CDS             complement(631..2937)
                     /locus_tag="VGO02_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEV7476287.1"
                     /translation="MPRASAAQTMLPLGDQPEEARGWDQILRAAFADPDRASGFLSQA
                     EEAVRAQPGDGLILLLAATAALLDRNPERAQVFLKRFSKRYVAIAPYHLLRALVLAEQ
                     SKLGSARSVLEAHGLTNPFNALQVFPGGWMRRAWLLRQHDRIFGHDKPVRGRGASDAG
                     RRPAQDQAKIKPNKSRDKSPPAPAAEAPPGLPLIDVDIPFSAELDLAPLVTAMRSAPE
                     SDGGWHGLRERFAHLGLAQGFDELLCLPHLRDIETLWYQVETVRKVLKQFRGRVLLAD
                     EVGLGKTIEAGMVLKEYLLRGMVDSVLVLAPASLVGQWREELETKFDIPCATTHDALL
                     RSDPDRFWNQRCLIASLALARRSEHAAPLIGRSFDLVIVDEAHHLRDRTSQSYKLVDA
                     LNKRFLLLLSATPVQNDLTELYNVLTLLKPGIFKTLKEFRGAYMTPGKPRQPANPERL
                     RALMRDAMVRNTRAVVALKLPQRHAVTIRVDGAEGERAAYQELAAAARRLGAEASGRN
                     RLALHHLLGAAGSSPAAAAAAVRRFAARHGDEPGWHVLAQRWASAGNGGKEAALLELL
                     RRNRDEKKLVFVHYRETLDHLAELLARAGFGFARFDGGLSGPDKDAAIAAFSESVPVL
                     LCTESGGEGRNIQFCNTLINFDVPWNPMAIEQRIGRIDRIGQQREVFVFNLVTRGTLE
                     EQILHLLDEKISMFELVVGEVGAILGGLEEDRDFADLMLDAWLNTTEASRNEAFDALG
                     HRLHAARQHHESAKALDESLFGEDFEAA"
BASE COUNT          564 a         1139 c         1119 g          572 t
ORIGIN      
        1 gtcgcgcacc tgccgatcga gtagcggacg caaccgcgct tcgagggtcg ccgcgctcca
       61 tcccgacccg gccgcgagcc tggtcgcctg atcgggcgcc tgccaatcca gtacttgcgc
      121 cagcgccggc cgcagacgca ccaggatgtc gctgacgacc gcgccggtgc cgaggttcaa
      181 gcccagccag atcagaccct cacgcttctc gtcggacacc gcggtgtagc ggaaggccag
      241 catcaggcag cgtgtccaag tcgcggtcat gccctggaaa cgccaaaccg cgttcggcag
      301 gtcgagcgca cggtcgagca gccgtccggg gtcgctcggc gccggaacgg caccagccgg
      361 actcaccgcc cgctcgctcc agcgtccctc gtcttcgagg agcgcgccga agcggtcgag
      421 ccagtcgccc tcgagcccga ttgggatcgc gccgtggctg cgctcccgcc cgaaactgag
      481 gcgcgtcagc tcgggccagc cggtcgcctt ctgcaccggt gccggcgcga gcacctcgag
      541 tccatccgga tccacagctt tgtcgactgc cgcgcctttg cgctccagca gatcggcgac
      601 gaaattccgt agctcgctca tcggcccgtc tcacgccgct tcgaagtctt cgccgaacag
      661 cgactcgtcc agggccttgg cgctctcgtg gtgctgcctc gccgcgtgca ggcgatggcc
      721 gagggcatcg aacgcctcgt tgcggctcgc ttcggtggtg ttgagccagg catcgagcat
      781 gaggtcggca aaatcgcgat cctcctcgag tccccccagg atcgccccca cttcgccgac
      841 cacgagctcg aacatggaga ttttctcgtc cagcagatgc agaatctgct cctctagcgt
      901 gccgcgggta acgagattga acacgaagac ctcgcgctgc tggcctatgc gatcgatgcg
      961 tccgatgcgc tgctcgatcg ccattggatt ccagggcacg tcgaaattga tcagcgtatt
     1021 gcagaactgg atattgcggc cttcgccgcc cgattcggtg caaagcagca ccggcacgct
     1081 ctcgctaaag gcggcgatgg cggcgtcctt gtcggggccg ctcaggccac catcgaagcg
     1141 cgcgaagccg aagcccgccc gtgccaacag ctcggcgagg tgatcgagcg tctcgcggta
     1201 gtggacgaag accagcttct tttcgtcccg attgcgccgc agcagctcga gcagggccgc
     1261 ctccttgccg ccgtttccgg cggacgccca gcgctgcgcc agcacgtgcc aaccgggctc
     1321 gtcgccatgg cgagcggcga agcgcctgac ggcagcggct gccgcggccg gagacgatcc
     1381 cgcggcgccg agcagatggt gcagcgcaag ccggtttctg ccgcttgctt cggcccccag
     1441 ccggcgcgcc gctgcggcca gctcctggta ggccgcccgt tcgccctcag cgccgtccac
     1501 gcggatcgtc acggcgtgac gttgcggcag cttcaaggcc acgacggcgc gcgtgttgcg
     1561 taccatggcg tcgcgcatca aggcccgcag gcgctcggga ttggccggct ggcgcggctt
     1621 gccgggggtc atataggcgc cacgaaattc cttcagcgtc ttgaagatgc ccggcttgag
     1681 cagcgtgagc acgttgtaga gctcggtgag atcgttctgc accggtgtcg ccgacagcag
     1741 aagcaggaag cgcttgttga gtgcgtccac caatttgtag ctctggctcg tgcggtcgcg
     1801 cagatgatgg gcctcgtcta cgatgactag gtcgaagctg cggccgatca ggggagcggc
     1861 atgttcgctc cgacgcgcca gcgcaagcga agcgatcagg catctctgat tccagaaccg
     1921 gtccgggtcg ctgcgcagca acgcgtcgtg ggtggtcgcg cagggaatgt cgaacttggt
     1981 ctcgagctcc tcccgccact gcccgacgag cgacgccggg gcgagcacga gcacgctgtc
     2041 gaccatgccg cgcagcagat actccttgag caccatgccg gcctcaatgg tcttgccgag
     2101 cccgacctcg tcggcgagca gcacccggcc gcgaaactgt ttgagcacct tgcgcacggt
     2161 ctcgacctgg taccacaagg tttcgatgtc gcgcagatgc ggcaggcaga gcagctcgtc
     2221 gaagccttgc gccaagccga gatgggcgaa gcgctcgcgc agcccgtgcc agccgccgtc
     2281 gctttccggc gcgctccgca tggcggtcac gagcggcgcg agatcgagtt cggcactgaa
     2341 cgggatatct acgtcgatca acggcagccc gggcggcgcc tcggcagctg gggccggcgg
     2401 gctcttgtcg cgagatttgt tcggcttgat cttggcctga tcttgcgccg gtctccgccc
     2461 ggcgtcggag gcaccgcgtc cgcggacagg cttgtcatgt ccgaagatgc ggtcgtgctg
     2521 gcgaaggagc catgcccgcc gcatccaacc gccgggaaag acctgcaggg cgttgaatgg
     2581 attggtgagc ccatgggctt ccagcacgga ccgcgccgat ccaagcttgc tttgttccgc
     2641 gagcaccaga gcgcgcaaca ggtggtaggg cgctatcgca acgtaccgct tggagaaccg
     2701 cttcaggaaa acctgcgccc gctcggggtt ccgatcgagg agcgcagccg tcgccgcgag
     2761 cagcagaatc aggccatcgc ccggctgcgc gcgcacggcc tcttcggcct gcgatagaaa
     2821 ccccgaggcg cggtccggat cggcgaaggc ggcgcgtagg atttgatccc aaccgcgggc
     2881 ctcctccggc tgatcgccga gaggtagcat cgtctgcgcc gcggaagcac gaggcatgct
     2941 ggactaaatc gtattgtctt cgccttcgaa caaagcggac gaatcgacga ctcgttagac
     3001 caaggttacc gggatacgac gtgcaatcaa gcgagccata gcggctcctg tggatcgccg
     3061 atcccggatt tggcgaatcg cgacgcgccc agaggctatc tgggtcaaat ctatggttcc
     3121 gtcgcctgcg agcgtgtggt gccggacgtg gaaggctttc acgcgctggc gctccagaga
     3181 cctatcaaac caccactcac cgagaaagag attggcgcca aaagagccac cggccctgcc
     3241 ggtggctctt ggtcttaggc gcatcgatgt tcgccgatga gcacggttgt gcgccgatgc
     3301 gtgcgcgccg ttaggacgaa gcgaaggctc gcgaacctct ggcgtctccg gtgcctttgc
     3361 ctccgcggcg gcggggcgta ggctgggcgg caat
//
LOCUS       DASYJA010000045         5813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1460056, whole genome shotgun sequence.
ACCESSION   DASYJA010000045 DASYJA010000000
VERSION     DASYJA010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5813
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460056"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VGO02_00960"
     CDS             complement(<1..378)
                     /locus_tag="VGO02_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476288.1"
                     /translation="MKLNKLIFAATLLAAASAQAQSTRGGKGELYLSPVFTDGKKYSF
                     EGGSTATTDTGYGLTLGYAFNFDQHRALGIEVSWADQDYRAIVQPGPGNIANSATDVR
                     GTLETWTVRFLGNYYFMEGNLTPF"
     gene            complement(422..1321)
                     /locus_tag="VGO02_00965"
     CDS             complement(422..1321)
                     /locus_tag="VGO02_00965"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEV7476289.1"
                     /translation="MRALALALLAPAACALAQSMEPRAYSAGPVGLNFAIAGYAYSTG
                     AVGFDATAPLQDGHIKVHSLPLAYVRTLDVLGNSGSIALVLPFASLSGSATLNGSAEV
                     TRDISGMADPAVRLAVNFYGAPAMSAAQFAGYRQDLIAGASLSISAPFGQYDPQRLVN
                     LGTNRWSIKPELGVSQALGPWTVELAAGATWFSRNDEFFQGNTREQEPIYSAQLHLTR
                     QFGRGLWGAVSATYYEGGRTTLNGVAQDDRQKGTRGGLTLALPLTRQQSIKLFYQSGL
                     YARAGTDFDTFGLAWQYRWGEGL"
     gene            complement(1324..1581)
                     /locus_tag="VGO02_00970"
     CDS             complement(1324..1581)
                     /locus_tag="VGO02_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476290.1"
                     /translation="MSDKRGIIIHFMDGSKKLLEFPQQVADADVAAAAKLKEALETRH
                     LVIQAEGALIVIPFDNVKYLQIYPAPKKLPGYAIRGASFKD"
     gene            complement(1607..2014)
                     /locus_tag="VGO02_00975"
     CDS             complement(1607..2014)
                     /locus_tag="VGO02_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476291.1"
                     /translation="MTRAVLACLASAALDAQAQTCTVCGEVRAIREVSAARPSSVAAP
                     RPPAAVGTTSALDTAPVVGTVARFEFDRDRPDSWYLGAAGTPEMRQRLGETYYELTVA
                     MDGGERRTVQRRDGNRFYVGQRVALRSGELEPM"
     gene            complement(2027..2878)
                     /locus_tag="VGO02_00980"
     CDS             complement(2027..2878)
                     /locus_tag="VGO02_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV7476292.1"
                     /translation="MRFLPLPLIVLALLAAAPASGQTLDKIRKQGTIKLGYIESAAPF
                     SYTESGSESQGYSIELCRAVADGLAAQLKRESLKIRWVKLTLQNRIEAVRRKEVDIEC
                     GTATWTLSRQQLVDFSLITFVDGASLLLRAQSDIGRMADLDGKRIAVIAGTTTEPALR
                     AALARQSVKAELVSIKTRDEGLDLLGRNEVEALASDRTALIGLVVMGGAGRGSFKLLD
                     QDFSIEQYALMLPRGDHDFRLAVNRVLARVYRSGEIEKIYARWLGPLGPPSMLLSATY
                     YIQSLSE"
     gene            complement(2887..3264)
                     /locus_tag="VGO02_00985"
     CDS             complement(2887..3264)
                     /locus_tag="VGO02_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7476293.1"
                     /translation="MPYVAIVDDEEPVRKALRRLMRAAGLQAEGFAGGREFLEAAADR
                     PPDCVVLDLHMAGMSGLQVLRELQATPQRPPVVIITAHDEPETREQCRAAGAAAYLRK
                     PLDDRLLLNAISAALSAAAQLRR"
     gene            complement(3328..5037)
                     /locus_tag="VGO02_00990"
     CDS             complement(3328..5037)
                     /locus_tag="VGO02_00990"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEV7476294.1"
                     /translation="MDSHSAYGRSIRALSAAGVQIPWLGRLEASWAAPAVMLALGYYL
                     AARVGFAFTLQPHPISTLWPPNALLMAALLLAPTRAWWLLLAAALPAHLLAELQSGVP
                     TAMVLGWYASNCSEALIGASLVRALIPGALRLDSFRNAGAFLLCGGLVAPLLSSFLDA
                     ALVRLIGWGSGDYLELVRMRVFSNVLAELTVAPLILTWAAFGMAGLREAPLHRYLEAA
                     VLFLGLTAVSLVAFDLPLDNASTAPALFYAPLPFLLWAAVRLGPAGTASALALVVVLT
                     IWGAVHGLGPFTGGTPQQTARDMQLFLTAVSVPMLLLAVVLEERSRVAHVAREQRLQL
                     THLSRVAMLGQLAGGIAHELNQPLTVILSNAQAAQRFIASRNVDPEELADILRDIISA
                     DQRAGEVIRRLRPLFRRDEAQFEPLDANEVVQEVLGIVHGDLVMRGVEVVTQLAAALP
                     PLEGDRVELQQVMLNLVLNACEAMSAGAREARRLTVRTRADEGGVQVSFADRGPGFAA
                     EQYDRLFQPFFTTKAQGLGLGLSISRSIVIAHGGRLWGSSIQGEGASFHIFLPAGRAG
                     AVG"
     gene            complement(4995..5615)
                     /locus_tag="VGO02_00995"
     CDS             complement(4995..5615)
                     /locus_tag="VGO02_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7476295.1"
                     /translation="MQACISVVDDDALVLRSLDRLLRSAGFAVRTFPSSQAFLADPEA
                     DPGCVVIDLSLPGLGGLELQQALARSADPRPVVFITGRGDIASSVAAMKAGAIDFLVK
                     PFDEQALLTAVRSAVEKDRAARGVREELSLIVGRLAALTPREREVLAGVVEGKLNKQI
                     AAELGTAEKTVKVHRARMMRKMGVASLAGLVRAWTAIRHTDAASAP"
BASE COUNT          895 a         1955 c         2098 g          865 t
ORIGIN      
        1 gaacggcgtg aggttgccct ccatgaagta gtagttgccg aggaagcgca cggtccaggt
       61 ttccagagtg ccgcgaacgt cggtggcgct gttggcaatg ttcccaggtc cgggttggac
      121 gatcgcgcgg taatcctggt cggcccatga cacctcgatg cccagggccc ggtgctggtc
      181 gaagttgaac gcgtagccaa gcgtcaggcc gtagcccgtg tcggtagttg cggtggagcc
      241 accttcgaac gaatacttct tgccgtcggt gaacacgggc gacaggtaga gctcgccctt
      301 gccgccgcgc gtcgactgcg cctgggcgga tgccgcggca agcagcgtag cggcaaagat
      361 cagcttgttc agcttcattg gtcagtctcc ctttgaaatg gtcggacttc tggaatcggt
      421 ctcacaggcc ctctccccag cggtattgcc aggcgaggcc gaaggtgtcg aaatcggtgc
      481 cggcgcgcgc gtaaaggccc gactggtaga acagcttgat cgattgctgc cgggtgagcg
      541 gcagcgccaa tgtcaggccg ccacgcgttc ccttctgccg gtcgtcctgg gcgacgccat
      601 tgagggtggt gcgtccaccc tcgtagtagg tggcgctcac cgcgccccag aggccgcggc
      661 cgaactgccg cgtgaggtgc aattgcgcag agtagatcgg ctcctgctcg cgcgtgtttc
      721 cctggaagaa ttcgtcgttc cggctgaacc aggtcgcgcc cgccgccagc tcgacggtcc
      781 acggccccag ggcctgcgac acgccgagct cgggcttgat cgaccagcgg ttggtgccca
      841 ggttgaccag gcgctgtggg tcgtactggc cgaagggagc gctgatcgac aggctcgcac
      901 cggcgatcag gtcctgccgg tagccggcaa attgcgccgc gctcatcgct ggggcgccgt
      961 agaagttcac tgcgaggcgt actgcgggat cggccatgcc ggagatatcg cgcgtcacct
     1021 cggcgctgcc gttgagggtc gcgctgccgg agaggctggc gaagggcagg accagcgcga
     1081 tgctgccgga gttgcccagg acatcgagcg tgcgcacgta ggcgagcggc agcgaatgca
     1141 cctttatgtg gccgtcttgc aagggcgcgg tagcgtcgaa gccgacggcg ccggtcgagt
     1201 aggcgtagcc ggcgatcgcg aaattcagcc cgactggccc ggccgagtag gcgcgcggct
     1261 ccatcgactg cgcgagcgcg caggccgccg gcgccagcag ggcgagcgca agggcgcgca
     1321 tgcttagtcc ttgaaacttg cccctcggat ggcgtagccg ggcagcttct tcggcgccgg
     1381 gtagatctgc aggtacttga cgttgtcgaa cggaatcacg atcagcgcgc cctcggcctg
     1441 aatgaccagg tggcgcgtct cgagcgcctc cttcagcttg gcggcggcgg cgacgtcggc
     1501 gtcggcgacc tgctgcggga actcgagcag ctttttcgac ccgtccatga agtggatgat
     1561 gatgccgcgc ttgtcgctca tgtggttctc tccgggatga tgggattcac atcggctcga
     1621 gctcgcccga gcgcagcgcg acgcgctggc cgacgtagaa gcggttgccg tcgcggcgct
     1681 gcacggtgcg gcgctcgccg ccgtccatcg ccacggtcag ctcgtagtag gtctcgccca
     1741 ggcgctggcg catctcgggc gtgccggcag cgccgagata ccagctgtcc gggcggtcgc
     1801 ggtcgaattc gaagcgcgcc acggtgccca ccaccggggc ggtatcgagc gcggaagtgg
     1861 tgccgaccgc cgcgggcggg cgcggcgcgg cgacactgga gggccgcgct gcgctgacct
     1921 cgcggatggc gcgcacttcg ccgcagacgg tgcaggtctg cgcctgcgcg tcgagcgccg
     1981 cgctggcgag gcaggcgagg acggcgcggg tcaaggcctg catcgcttat tcggacagcg
     2041 actggatgta gtaggtggcc gagagcagca tgctcggcgg gccgagcggc ccgagccagc
     2101 gggcgtagat cttctcgatc tcgccgctgc ggtagacgcg cgccagcacc cggttcaccg
     2161 ccaggcggaa gtcgtggtcg ccgcgcggca gcatgagcgc gtactgctcg atggagaagt
     2221 cctggtcgag cagcttgaag ctgccgcgtc ctgcgccgcc catcaccacc agcccgatga
     2281 gcgcggtgcg gtcggaggcg agcgcctcga cctcgttgcg gccgagcagg tcgaggccct
     2341 cgtcgcgcgt cttgatgctc accagctcgg ccttgaccga ttggcgcgcc agggccgcgc
     2401 gcagcgccgg ctcggtagtg gtgccggcga tcaccgcgat gcgtttgccg tcgaggtcgg
     2461 ccatgcggcc gatgtcggac tgcgcgcgca gcagcaggct ggcgccgtcg acgaaggtga
     2521 tcaggctgaa gtcgacgagc tgctggcgcg acagggtcca ggtcgcggtg ccgcattcaa
     2581 tgtccacttc cttgcggcgc acggcctcga tgcggttctg cagcgtcagc ttgacccagc
     2641 ggatcttgag gctctcccgc ttgagctgcg cggcgaggcc gtcggcgacc gcgcggcaca
     2701 gctcgatcga gtagccctgc gactcgctgc cgctctcggt gtaggagaaa ggggcggcgc
     2761 tctcgatgta gccgagcttg atcgtgccct gcttgcggat cttgtcgagc gtctggccgc
     2821 tggcgggggc ggcggcaagc agcgccagga caatgagcgg caggggcagg aatcgcatgc
     2881 ggcgggtcat ctccggagtt gggccgcggc gctcagcgcc gcggaaatgg cattgagcag
     2941 cagccggtcg tcgagcggct tgcgcaggta ggcggcggcg cccgcggcgc ggcactgctc
     3001 gcgcgtctcg ggctcgtcgt gcgcggtgat gatcacgacc ggcggacgct gcggcgtggc
     3061 ctgcagctcg cgcagcacct gcaggccgct catgcctgcc atgtgcaggt cgagcaccac
     3121 gcagtcgggc ggccggtcag cggcggcctc aaggaactcc cggccgccgg caaagccctc
     3181 ggcctgcagc ccggcggcgc gcatcagccg ccgcagggcc ttgcggaccg gctcctcgtc
     3241 gtcgacgatc gccacgtacg gcatgcagcg attggagcag gtcgcgggcc cctcagtaat
     3301 tggaccaagg tcccataccg gtgcggctca gccgacggcg ccggcgcggc ccgctggcag
     3361 gaagatatgg aagctcgctc cctcgccctg gatagagctg ccccacagcc tgccgccatg
     3421 ggcgatgacg atcgagcgcg agatcgacag ccccaggccg aggccctgcg ccttggtggt
     3481 gaagaagggc tggaacagcc tgtcgtactg ctcggcggcg aagccggggc cgcggtcggc
     3541 gaagctgacc tgcacgccgc cctcgtcggc gcgcgtccgg acggtgagcc ggcgcgcctc
     3601 gcgcgcgccg gcgctcatcg cctcgcaggc gttgagcacc aggttgagca tcacctgctg
     3661 cagctcgacg cggtcgcctt cgagcggcgg tagcgccgcc gcgagctgcg tcacgacctc
     3721 gacgccgcgc atcaccaggt cgccgtgcac gatgccgagc acctcctgca ccacctcgtt
     3781 ggcgtcgagc ggctcgaatt gcgcctcgtc gcgcctgaag aggggccgca gccgccggat
     3841 cacctctccg gcgcgctggt cggcggaaat gatgtcgcgc aggatgtcgg ccagctcctc
     3901 ggggtcgaca ttccggctgg cgatgaagcg ctgcgcagcc tgcgcgttgc tgaggattac
     3961 cgtgagcggc tggttgagct cgtgggcaat gccgcccgcg agctggccga gcatcgccac
     4021 gcggctgagg tgggtgagct gcaggcgctg ctcgcgggcg acgtgcgcga cgcggctgcg
     4081 ctcctcgagg actaccgcga gcagcagcat cggcaccgac accgcggtca ggaatagctg
     4141 catgtcgcgc gccgtctgct gcggcgtgcc gccggtgaac gggcccaggc cgtggaccgc
     4201 gccccagatg gtcagcacca cgaccagtgc gagggcactc gccgtccccg ccgggccgag
     4261 gcgcaccgcg gcccatagca ggaacggcag cggcgcgtag aacagcgccg gcgcggtgct
     4321 cgcattgtcc aggggcaggt cgaaagccac caggctcacg gcggtgaggc cgaggaacag
     4381 caccgccgcc tcgaggtagc ggtgcaaggg cgcttcgcgc agccccgcca tgccgaaggc
     4441 cgcccaggtc aggatgagcg gcgcgacggt gagctcggcc agcacgttgg agaagacgcg
     4501 catgcgcacc agttccaggt agtcgccgct gccccagccg atcagcctga cgagcgcggc
     4561 gtccaggaaa gacgagagca gcggcgccac cagcccgccg cacagcagga aggcgccggc
     4621 gttgcggaac gagtcgaggc gcagcgcgcc gggaatcagg gcgcgcacca gggacgcgcc
     4681 gatcagcgcc tcgctgcagt tgctggcata ccagccaagg accatcgccg tcggcacgcc
     4741 gctttgcagc tcggccagca ggtgcgccgg cagcgctgcg gcgagcagca gccaccaggc
     4801 gcgcgtcggc gcgagcagca gcgccgccat cagcagggca ttcggcggcc acagggtcga
     4861 gatcgggtgc ggctgcagcg taaaggcgaa gccgacgcgc gcggcgaggt agtagccgag
     4921 cgcgagcatg accgccggcg ccgcccagct cgcttccagg cggccgagcc acgggatctg
     4981 tactcctgcc gcgctcaggg cgcggatgct gcgtccgtat gccgaatggc tgtccatgcc
     5041 cgaaccagtc cggcgagcga ggcgacgccc atcttgcgca tcatgcgggc gcggtgaacc
     5101 ttgacggtct tctcggcggt gccgagctcg gcggcgatct gcttgttcag cttgccctcc
     5161 accacgccgg cgagcacttc gcgctcgcgc ggggtcagcg cggcgaggcg gcccacgatc
     5221 aacgagagct cctcgcgcac gccgcgcgcc gcgcgatctt tctccaccgc cgagcgcacc
     5281 gccgtcagca gcgcctgctc gtcgaacggc ttcaccagga aatcgatcgc gcccgctttc
     5341 atcgccgcca cgctgctcgc gatatcgccg cgtcctgtga tgaatacgac cgggcgcggg
     5401 tcggccgagc gcgcgagcgc ctgctgcaac tcgaggccgc ctaggccggg cagcgacagg
     5461 tcgatcacca cgcagcccgg atcggcctcg gggtcggcga gaaacgcctg cgaagaaggg
     5521 aaagtgcgca cggcgaagcc cgccgagcgc agcagccggt cgagcgacct gagcaccaag
     5581 gcatcgtcgt cgacgacgct gatacaggct tgcactgcaa cctccctttt ccccgagtcg
     5641 aggctacacc caaagcgtta cgggaggaag accggaattt tttaccgatc cttcgggcgg
     5701 cgatactgca gcaggccgcc gacgcggcgc tcgaacgggt cgcgccgcgt caccgcactc
     5761 atatggtgcc cgttcaccag cgggaagtgg cgcagcgcgt cgagcgcctc gca
//
LOCUS       DASYJA010000046         4735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2095887, whole genome shotgun sequence.
ACCESSION   DASYJA010000046 DASYJA010000000
VERSION     DASYJA010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2095887"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1551)
                     /locus_tag="VGO02_01000"
     CDS             complement(<1..1551)
                     /locus_tag="VGO02_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEV7476296.1"
                     /translation="MNAPTDLARFSLDDKYTVERGRVFLTGTQALIRLLMLQRTRDER
                     AGLNTAGFVSGYRGSPLGGLDQALWRAQKHLESHHVKFQPGVNEDLAAAAIWGTQQVT
                     MFPKAKYDGVFGMWYGKGPGVDRCGDVFKHANAAGTSKHGGVLVLAGDDHAAKSSTLP
                     HQSEHIFKACLIPHLNPASVQDYLDLGLHGYAMSRYSGCWVAFKCVTDVVESGASVLV
                     DPDRINVRIPTDFVAPADGLNIRWPDGILEQEARILDWKVYAALAYVRANGLDKVIFD
                     SPKARLGIVTAGKSYGDVMQALADLGIDAQTARDIGLRVYKVAMTWPLEPQGARRFAE
                     GLEEVLVVEEKRQLIEYQIKEELYNWKEGVRAPRVVGKFDDSGEWSRSGGKPAGTWLL
                     PAQYEHSPAMVARAIAQRLEKLGMTGPLGSQFRERLAFLDFKEKLLAKPRVTAVRQPY
                     FCSGCPHNTSTRVPEGSRATAGIGCHFMAVWMDRSTATFTHMGAEGAPWIGQAPFTDE
                     KHIFANLGD"
     gene            complement(1565..2515)
                     /locus_tag="VGO02_01005"
     CDS             complement(1565..2515)
                     /locus_tag="VGO02_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV7476297.1"
                     /translation="MADSFSEMAVFAKVVTSGSLSAAARELSLSPAMVSRRLAALEAR
                     LGVRLVNRTTRSLHLTDEGAGYYESCARVLAEIEQANASVSAGRREPQGTLRVALPAA
                     FGNQYVAPLVPQFAALYPAVRLALSLSDRSINLVEDGFDLAIRIAALADSSLAARKLA
                     PNRRVVCASPEYLRRHGTPSAPVELAQHNCLLASDFGATWDYKNASGKLGSVRVSGRY
                     VCDNWEVLREWALAGLGIALKSTWDVRRHLSSGSLVSLLPGYSFDSDVAIYAVYPHRR
                     HLPAKTRAFIDFLASSFGPEPFWDRVVVKPRIKARSGSAP"
     gene            complement(2672..3361)
                     /locus_tag="VGO02_01010"
     CDS             complement(2672..3361)
                     /locus_tag="VGO02_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7476298.1"
                     /translation="MRIAILEDDPDQLALLRRWITDDGHDVHAYVSGREAMKMAGRES
                     FDLFMLDWQVPDVTGAEVLMWVRANVSKSVPVLFVTVRDSEQDIVFALEHGADDYMVK
                     PVRRQELLARVHALLRRAYPSEEKKLLSFPPFEIDTQRNEVRKEGAKLDLTPKEFELT
                     VTLFRNIGRLMSRGHLQETVWGRSGDLATRTVDTHVSQVRKKLDLRPESGYRVVPIYN
                     YGYRLEKITPP"
     gene            3416..4234
                     /locus_tag="VGO02_01015"
     CDS             3416..4234
                     /locus_tag="VGO02_01015"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF009828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HEV7476299.1"
                     /translation="MRIALIGGGTIARLVLEHARRASLGRFEIVALMGRPGGAARARE
                     LAREFSVKYVENAAALASERPQAVIEAASHAAVKEHLVGLLDNGIAVVVLSAGALIDD
                     ALREAAEAAARRSGALLFVPSGGIGGLDALKTACLAGVDEASIEVAKPPAAWKGIAYV
                     ESRGFALDDLRSPLTLFEGTAREGVPHFPQNVNIAAILALAGIGPDRTRLKVVADPAL
                     ALNTHTIRVSGRSGRFTLRLENVPAPENPKTSWLACYSALAALQALGSPVRYGT"
     gene            4294..4380
                     /locus_tag="VGO02_01020"
     tRNA            4294..4380
                     /locus_tag="VGO02_01020"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4328..4330,aa:Tyr,seq:gta)
     gene            4440..4513
                     /locus_tag="VGO02_01025"
     tRNA            4440..4513
                     /locus_tag="VGO02_01025"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4472..4474,aa:Gly,seq:tcc)
     gene            4603..4677
                     /locus_tag="VGO02_01030"
     tRNA            4603..4677
                     /locus_tag="VGO02_01030"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4635..4637,aa:Thr,seq:ggt)
BASE COUNT          790 a         1494 c         1585 g          866 t
ORIGIN      
        1 gtcgccgagg ttggcgaaga tgtgcttttc gtcggtgaac ggcgcctgcc cgatccacgg
       61 cgcgccttcg gcgcccatgt gggtgaaggt cgcggtgctg cggtccatcc ataccgccat
      121 aaagtggcag ccgatgccgg cggtggcgcg gctaccttcg ggcacccggg tggacgtgtt
      181 gtgcgggcag cccgagcaga aatacggttg cctgaccgcc gtcactctcg gtttggcgag
      241 cagcttctcc ttgaaatcga ggaacgcaag ccgctcgcgg aactgcgacc cgagcggacc
      301 tgtcatgccc agcttttcca gtctctgggc aatggcgcgg gccaccatgg ccggagaatg
      361 ctcgtactgc gcgggaagca gccacgtccc ggccggcttg ccgccgctgc ggctccattc
      421 gccgctgtcg tcgaacttgc ccacgacgcg cggcgcgcgc acgccttcct tccagttgta
      481 gagctcctcc ttgatctggt actcaatcag ctggcgcttc tcttccacca ccaggacttc
      541 ttcgagcccc tcggcgaagc ggcgcgcgcc ctgcggctcg agcggccacg tcatcgccac
      601 cttatagacg cgcagcccga tgtctctcgc ggtttgcgcg tcgattccca ggtccgcgag
      661 cgcctgcatc acgtcgccgt aggacttgcc ggcggtgacg atgcccaggc gcgccttcgg
      721 cgaatcgaag atcaccttgt ccaggccgtt ggcgcgcacg tacgcgagcg ctgcgtacac
      781 cttccagtcg aggatgcgcg cttcctgctc gaggatgccg tcgggccagc ggatgttgag
      841 cccgtcggcg ggcgcaacga aatcggttgg gattcttacg ttgattctgt ccggatcaac
      901 cagcaccgaa gcgccggatt cgaccacgtc ggtgacgcat ttgaaagcga cccagcagcc
      961 ggaatagcgg ctcatcgcgt agccgtgcag tccgaggtcg agataatcct gcacgctcgc
     1021 cgggttgagg tgcgggatga ggcaggcctt gaagatgtgc tccgactggt gcggcagcgt
     1081 ggaggacttc gcggcgtggt cgtcgcccgc gagcaccagc acgccgccat gcttcgaagt
     1141 gcccgcggca ttggcgtgct tgaagacgtc gccgcagcgg tccactccgg ggcccttgcc
     1201 gtaccacatg ccaaagacgc cgtcgtattt cgccttcggg aacatcgtca cctgctgcgt
     1261 gccccagatc gcggccgcgg cgaggtcctc gttgaccccg ggctggaatt tgacgtggtg
     1321 gctctcgagg tgtttctgcg cgcgccacag cgcctggtcg agcccgccga gcggcgagcc
     1381 gcggtagccc gacacgaaac ccgcggtgtt gaggcccgcc ctctcatcgc gcgtgcgctg
     1441 cagcatgagc aggcggatga gcgcctgcgt tccggtgagg aacacgcggc cgcgttcgac
     1501 ggtgtacttg tcgtcgaggc tgaaccgcgc gagatcggtg ggggcgttca tgcagcaggg
     1561 tccattatgg cgctgaaccg gatcgagcct tgatgcgcgg cttgacgacc acgcggtccc
     1621 agaaaggttc ggggccgaac gagctcgcga gaaaatcgat gaaggcgcgc gtcttggccg
     1681 gaaggtgacg ccgatgagga tagaccgcat aaattgccac atcgctgtcg aagctatagc
     1741 ccggaagtag cgacaccagc gagccgctcg agagatggcg gcgcacgtcc caggtcgact
     1801 tgagcgcgat gccgagcccg gcgagcgccc attcgcgcag gacctcccag ttgtcgcaga
     1861 cataacggcc gctcacgcgc accgagccga gcttgccgct ggcattcttg tagtcccagg
     1921 tcgcgccgaa gtccgatgcg aggaggcagt tgtgctgcgc gagctcgacc ggagcgctcg
     1981 gcgttccgtg gcggcgcagg tactccggac tcgcgcagac cacgcgccga ttgggggcga
     2041 gcttgcgcgc cgcgagggag gagtcggcca gcgctgcgat ccggatcgcg agatcgaagc
     2101 catcctccac caggttgatg ctgcggtcgg agaggctgag ggccagccgt accgccggat
     2161 agagcgcggc gaactgcggc acgagcggcg cgacatactg gttgccgaac gccgccggca
     2221 gcgcgacccg cagcgtgcct tggggttccc ggcgccccgc agagaccgag gcattcgcct
     2281 gctcgatctc ggcaaggaca cgcgcgcagc tctcgtagta gcctgcccct tcgtcggtga
     2341 ggtgaaggct gcgcgtggtg cggttcacca gccgcacgcc aaggcgtgct tcgagggcgg
     2401 cgagcctgcg gctcaccatg gctggggaca ggctgagttc tcgcgcagcg gcagacagac
     2461 ttccgctagt gactaccttg gcgaacaccg ccatctcgct gaaggaatcg gccacctttg
     2521 cattctatgc aaaagtgttt tgcggagaat tcccggtgtt gctattccag ttaaaggctg
     2581 ctactgttgc attgcagcac cggtcgcctc ctcctcctcc tctggcaccg gagctctaag
     2641 ggcggcccct tcggggtcgc ccttattttt tctacggggg ggtgatcttc tcgaggcggt
     2701 agccgtagtt gtagatgggg actacgcggt accccgactc cgggcgcaga tcgagcttct
     2761 tgcggacctg gctgacgtgc gtgtccacgg tgcgcgtggc gaggtcgccg cttcttcccc
     2821 aaaccgtttc ctggagatgg ccgcgcgaca tcagccggcc gatgttgcga aacagcgtca
     2881 ctgtgagctc gaactccttg ggcgtcagat ccagcttggc gccctccttg cggacttcgt
     2941 tgcgctgggt gtcgatctcg aacggcggga aagacaggag cttcttctcc tcgctcggat
     3001 aggcgcggcg cagcaaggcg tgcacacggg cgagcagctc ctggcgccgc accggcttga
     3061 ccatgtagtc gtcggcccca tgctccaggg caaagacgat atcctgctcg gaatcgcgca
     3121 cggtgacgaa aaggaccggc acgctcttgg agacgttggc gcgcacccac atcagcacct
     3181 cggcgccggt gacgtccggg acctgccagt cgagcatgaa aaggtcgaag ctctcgcggc
     3241 cggccatctt catggcttcc cgcccgctca cgtaggcatg cacgtcgtgg ccgtcgtcgg
     3301 tgatccagcg gcgcaggagc gccagctggt cgggatcgtc ttctagaatc gcgatgcgca
     3361 ttgttctctc ccagccggca atgatactgc tatacggaaa tacgataagg aaatcatgcg
     3421 cattgccctg atcggaggag ggactatcgc gcggctggtg ctggaacacg cgcgccgcgc
     3481 cagcctggga cgcttcgaga tcgtggcgtt gatgggccgt cccggcggtg ccgcgcgcgc
     3541 acgcgagctc gcgcgcgagt tttccgtgaa atatgtcgag aatgcggcgg cgctggcgtc
     3601 agaaaggccg caggccgtga ttgaagcagc ttcgcacgcg gcggtgaagg aacatctcgt
     3661 cgggctgctg gacaacggca tcgccgtagt cgtgctctct gccggtgcgc tgatcgacga
     3721 cgcgctgcgc gaggccgcgg aagcggcggc gcgccgaagc ggggcgctgc tgttcgtgcc
     3781 gtcggggggc atcggcgggc tcgatgcgct caagaccgcc tgtctcgccg gcgttgacga
     3841 agcctcgatc gaggtcgcca agcctccggc ggcttggaag ggaatcgcct atgtcgagag
     3901 ccgcggcttc gcgctcgacg atctgaggag cccgctcacg ttgttcgaag gcactgcgcg
     3961 cgagggcgtg ccgcacttcc cgcagaacgt gaacatcgcg gcgatcctag cgctggccgg
     4021 cataggcccc gaccgcacgc ggctcaaggt ggtggccgac ccggcgctcg cgctcaacac
     4081 ccacaccatc cgtgtttcgg gacgctccgg gcgcttcacg ctgcggctgg agaacgttcc
     4141 cgcgcccgag aacccgaaga cctcctggct cgcgtgctac agcgcgctcg cggcgctgca
     4201 ggcgctcggc tcgccggtgc gctatgggac ataggaaatt ccgccggttt cttgaccaac
     4261 cgtcggataa ttcgcataat ttcgggttcc gccggagggg tgcccgagcg gctaaagggg
     4321 gcagactgta aatctgttgg ccatgcgcct acgtaggttc gaatcctacc ccctccacca
     4381 ggatttgcgg gatgcaaggg cgccaggatt tcgaagcgga aactcgatgc gaggcgcggg
     4441 cgggtgtagc tcaatggtag agcagaagcc ttccaagctt acgacgaggg ttcgattccc
     4501 ttcacccgct ccagtacact gttgtcggcg acagtgcgaa agtgttgttg gttggccgag
     4561 attgcaggat tttgttgtca aagcattatt gagagagccg cagcccatgt agctcagtgg
     4621 cagagcactc ccttggtaag ggagaggtcg tgcgttcaat ccgcgccatg ggcaccagaa
     4681 ttttgtcggc actgcatgtg gcatcggaat ttgcagtcga aggtccagat tttgc
//
LOCUS       DASYJA010000047         2192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1188221, whole genome shotgun sequence.
ACCESSION   DASYJA010000047 DASYJA010000000
VERSION     DASYJA010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188221"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VGO02_01035"
     CDS             complement(<1..249)
                     /locus_tag="VGO02_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476300.1"
                     /translation="MAARAPRRRRRRVPARDGLLGRVTMAKVAVLGAGAWGTAMAAVL
                     SGRLEVSLWARDRAQAEAIGRTRRNERYLPGIEVPRAVD"
     gene            complement(180..623)
                     /locus_tag="VGO02_01040"
     CDS             complement(180..623)
                     /locus_tag="VGO02_01040"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV7476301.1"
                     /translation="MRLLAAALCLLALPAAAGQFRAVGENAAVLYDAPSRQATPLFVA
                     TRYYPLEIVVELEAWAKVRDHTGAISWVEKRLLTDKRMVVVTAASAEAHQRPDDAAPV
                     SFVAAQNVALELLEPAQEGAPPAWLHVRHADGADGFLRATACWGE"
     gene            complement(631..1128)
                     /gene="secB"
                     /locus_tag="VGO02_01045"
     CDS             complement(631..1128)
                     /gene="secB"
                     /locus_tag="VGO02_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEV7476302.1"
                     /translation="MTDPAQAVPPQGSQPSLQIEKLYVKDISLEVPHAPEVFTQQAQP
                     EVEVQISTSAKQFGEGYFEVSIGATVTARAGERTLFLVEAAQAGIFSLRNVPAEQLDA
                     LLGIACPTILFPYLREAISDLVVRAGFPPVLLSPVSFEALYMQRMQQQAGQQRGAGPQ
                     IEIAR"
     gene            complement(1125..1385)
                     /gene="grxC"
                     /locus_tag="VGO02_01050"
     CDS             complement(1125..1385)
                     /gene="grxC"
                     /locus_tag="VGO02_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEV7476303.1"
                     /translation="MARVLMYCTAACPFCQSAERLLVEKGARIEKVRVDLEPGRRGEM
                     TQKSGRHTVPQIWIGERHIGGCDDLHALDRKGGLDSLLEATS"
     gene            complement(1389..1808)
                     /locus_tag="VGO02_01055"
     CDS             complement(1389..1808)
                     /locus_tag="VGO02_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV7476304.1"
                     /translation="MEYILEFIKNNLLLFAVAFASGAMLLWPLVRRSSGGPWVSPAQA
                     THLINREDALVLDVRDPGEYGASHALGAKNLPLSRLESGAGDVAKKKDATLILYCERG
                     DLSGKAAAALKKQGYTKVVNLSGGFGAWKQAGLPVER"
     gene            1836..>2192
                     /locus_tag="VGO02_01060"
     CDS             1836..>2192
                     /locus_tag="VGO02_01060"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEV7476305.1"
                     /translation="MPKLVLLRHGESHWNRENRFTGWVDVDLSAAGIEQARTAGRTLK
                     AAGYRFDLAFTSVLKRAIRTLWIGLEELDQMWLPVEKSWRLNERHYGNLQGLNKAEMA
                     AKFGEDKVLVWRRSYDV"
BASE COUNT          336 a          786 c          748 g          322 t
ORIGIN      
        1 gtcgaccgcg cgcggcacct cgatgccggg caggtagcgc tcgttgcggc gcgtgcgccc
       61 gatcgcctcg gcttgcgcgc ggtcgcgcgc ccagagcgac acttcgagcc gcccggaaag
      121 cactgcggcc atcgccgtgc cccaggcccc ggcgccaagc accgccacct tcgccatcgt
      181 cactcgcccc agcaggccgt cgcgcgcagg aacccgtcgg cgccgtcggc gtggcgcacg
      241 tgcagccatg ccggcggcgc gccctcctgc gccggctcga gcagctcgag ggccacgttc
      301 tgcgcggcga cgaacgacac cggcgcggcg tcgtcggggc gctggtgcgc ctcggccgag
      361 gccgcggtca ccaccaccat gcgcttgtcg gtcagcagcc gcttctccac ccaggagatg
      421 gcgccggtgt ggtcgcgcac cttggcccag gcctcgagct cgaccacgat ctcgagcggg
      481 tagtagcgcg tcgcgacgaa cagcggcgtc gcctgcctgg aaggcgcgtc gtacagcacc
      541 gccgcgttct ccccgacggc gcgaaactgg ccggccgcgg ccggcagcgc gagcaggcac
      601 aacgcggcgg cgagcaatct caccctgcgc tcaccgcgcg atttcgatct gcggccccgc
      661 gccccgctgc tggccggcct gctgctgcat gcgctgcatg tacagcgcct cgaaagacac
      721 cgggctgagc agcaccggcg ggaagccggc gcgaaccacg agatccgaga tcgcctcgcg
      781 caggtacggg aagaggatcg tcgggcaggc gatgccgagc agcgcatcga gctgctcggc
      841 gggcacgttg cgcagcgaga agatgccggc ctgcgcggcc tcgaccagga acagcgtgcg
      901 ctcgcccgcg cgcgcggtca ccgtcgcgcc gatgctgact tcgaagtagc cctcgccgaa
      961 ctgcttggcc gacgtgctga tctgcacctc cacttccggc tgcgcctgct gcgtgaagac
     1021 ttccggcgcg tgcggcacct cgagcgagat gtccttcacg tacagcttct cgatctgcag
     1081 gctcggctgc gagccctgcg gcgggacggc ttgcgctggg tcggtcatga ggtggcctcg
     1141 agcagggagt cgagcccgcc cttgcggtcg agcgcgtgaa gatcgtcgca gccgccgata
     1201 tggcgctcgc cgatccagat ctgcggcacg gtatggcggc cgctcttttg cgtcatttcg
     1261 ccgcgccgcc cgggctcgag atcgacgcgc accttctcga tgcgcgcgcc tttctccacc
     1321 agcaggcgct cggcggactg gcagaacgga caggcggcgg tgcagtacat cagcacgcgg
     1381 gccatgcgct agcgctccac cggcaggccg gcctgcttcc aggcgccgaa accgccggac
     1441 aggttcacca ccttggtgta gccctgcttc ttcagcgctg cggcggcctt gccggagaga
     1501 tcgccgcgct cgcagtacag gatcagcgtg gcgtccttct tcttcgccac gtcgcccgcg
     1561 ccagactcga ggcgcgagag cggcaggttc ttcgcgccga gggcgtggct ggcgccgtac
     1621 tcgcccgggt cgcgcacgtc gagcaccagc gcatcctcgc gattgatgag atgggtggcc
     1681 tgcgcggggc tcacccacgg cccgccggag ctgcggcgca cgagcggcca cagcagcatc
     1741 gcgcccgagg cgaaggcgac ggcgaacaga agaagattgt tcttaatgaa ttccaagatg
     1801 tattccacgg gaagccggtc attataattc acgcgatgcc caagctcgtc cttttgcgtc
     1861 acggcgaatc gcactggaac cgcgagaacc gcttcaccgg ctgggtcgac gtcgacctgt
     1921 ccgcggcggg catcgagcag gcgcgcaccg ccggccgcac cctcaaggcc gccggctacc
     1981 gcttcgacct cgccttcacc tcggtgctca agcgcgcgat ccgcacgctg tggatcggcc
     2041 ttgaggagct cgaccagatg tggctgccgg tcgagaagag ctggcggctg aacgaacgcc
     2101 actacggcaa cctgcagggc ctcaacaagg ccgagatggc ggcgaaattc ggcgaggaca
     2161 aagtgctggt ctggcggcgc agctacgacg tg
//
LOCUS       DASYJA010000048         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1006833, whole genome shotgun sequence.
ACCESSION   DASYJA010000048 DASYJA010000000
VERSION     DASYJA010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006833"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..546)
                     /locus_tag="VGO02_01065"
     CDS             complement(73..546)
                     /locus_tag="VGO02_01065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HEV7476306.1"
                     /translation="MARKTPRSAARKLYRVGRSQTGLGLFATAAIKNGAFIIAYRGRL
                     LTHAQAAKLEARGNRYMYEINSRWTVDGSSRRNIARYANHSCRPNAESDVVKRNVILR
                     AIRNIKPGDEITYDYGSEYFEIFLKPVGCKCVKCAEKRRRQRRERRLKSKRKTKR"
     gene            complement(660..1223)
                     /locus_tag="VGO02_01070"
     CDS             complement(660..1223)
                     /locus_tag="VGO02_01070"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEV7476307.1"
                     /translation="MVKVCDCFPELKDITDARERRYRSHPDKPAPEGPRSFVEVKVTS
                     IGQLNELVDCKKSGYKFIISEPVHVGGQNCAPTPLEFLLSGAVGCYAAVFAFYAAKLG
                     VSYDSFEAVARTDFDVRGHMMADAPTSAFQKVTISISVVSDAPEDKLKEVERLSFEGC
                     PGINTLREPVPVETRLTVTRTPSEQAA"
     gene            complement(1375..>2009)
                     /locus_tag="VGO02_01075"
     CDS             complement(1375..>2009)
                     /locus_tag="VGO02_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="epoxyalkane--coenzyme M transferase"
                     /protein_id="HEV7476308.1"
                     /translation="PSDEAYLYALADVMAREYRAIVDAGFILQLDCPDLALSRHMVFA
                     HLSLDDFRKVITTHVEALNHAVRDIPPERMRMHICWASTQGPHHKDVPLKDIVDIVLK
                     GRPQAVSFPGANPRHGHEWKVWKDVKLPDGKIIIPGVIDSTSNFVEHPELVADRLVQY
                     ARVVGPENVIGGVDCGFGTFAGRLQVDSKIVWMKLASLAEGAQLASRELW"
BASE COUNT          312 a          658 c          644 g          395 t
ORIGIN      
        1 tcttgcaacc atgtgcgcct ccgcgcatcc gcggcacgcc cgccaacggc gcttgctccg
       61 gcggctatcg cttcaccgct tggtcttgcg cttggacttg agccgccgct ccctccgctg
      121 cctgcgccgc ttctccgcgc atttgacgca tttgcatccg acgggcttga ggaagatctc
      181 gaaatattcc gacccgtagt cgtaggtgat ctcgtcgccc ggcttgatgt tccggatcgc
      241 ccgcaggatc acgttgcgtt tgaccacgtc ggattcggcg ttcggacgac aggagtggtt
      301 ggcatagcgg gcgatattgc ggcggctcga gccgtccacg gtccagcggc tgttgatctc
      361 gtacatgtag cggttgccgc gcgcctcgag cttggcggcc tgcgcatggg tgaggaggcg
      421 gccgcggtag gcgatgatga acgcgccgtt cttgatggcg gcggtggcga acaacccgag
      481 cccggtctgc gaacggccga cgcggtaaag cttgcgcgct gcgctgcgcg gggtcttgcg
      541 tgccatgacc actgacgctc agagcgggat gatttttgtt cgacgcatcg tcccgctcca
      601 gctttttgtt cgtgcgtgat cttttccgaa aaccggcgtc cacttttcgg gatcacgcac
      661 tacgccgcct gctccgatgg cgtgcgcgtc accgtcagcc gggtctccac cggcaccggc
      721 tcgcgcaggg tgttgatgcc ggggcagcct tcgaacgaca ggcgttcgac ctccttgagc
      781 ttgtcctcgg gcgcatcgct gaccacgctg atgctgatcg tcaccttctg gaacgccgac
      841 gtcggcgcat ccgccatcat gtggccgcgc acgtcgaagt ccgtgcgcgc caccgcctcg
      901 aagctgtcgt agctcacccc gagcttggcc gcgtagaagg cgaacaccgc cgcatagcag
      961 ccgaccgcgc cggagagcag gaactcgagc ggcgtcggcg cgcaattctg cccgcccaca
     1021 tgcacgggtt cgctgatgat gaacttgtag ccgctcttct tgcagtcgac gagctcgttg
     1081 agctggccga tcgaggtcac cttcacttcc acgaagctgc gcggcccttc gggcgccggc
     1141 ttgtcgggat gcgaacggta ccgacgctcg cgcgcgtcgg tgatatcctt caactcgggg
     1201 aagcaatcgc agaccttgac cattcaatct ctcccgggcg cgcttgcggc atgtcctgcg
     1261 cgctgtcctg cgcgcgaact tggcgagtga atgcccgcgc cgtcaagcct cgggttgctg
     1321 atcccggtgt cccggacgag cgagcgtgcg atcactcgct cctcataacc cgcctcacca
     1381 caactcgcgc gacgccagct gcgctccttc ggcgagcgac gcgagcttca tccatacgat
     1441 cttggaatcg acctgcaatc ggccggcgaa ggtgccgaag ccgcaatcga cgccgccgat
     1501 gacgttctcg ggtccgacca cgcgcgcata ttgcaccagc cggtccgcca ccagctccgg
     1561 gtgctccacg aagttcgagg tggaatcgat cacgccgggg atgatgatct tgccgtcggg
     1621 cagcttgacg tctttccaga ctttccactc gtgcccgtga cgcgggttgg cgccgggaaa
     1681 cgagaccgcc tggggccggc ccttgagcac gatatcgacg atgtccttga gcgggacgtc
     1741 cttgtggtgg gggccctggg tagatgccca gcagatgtgc atgcgcatgc gctcgggcgg
     1801 gatgtccctg accgcatggt tgagcgcctc gacgtgggtg gtaatcacct tgcggaaatc
     1861 gtccaggctc aaatgcgcga acaccatgtg gcggctcaac gccaggtcgg ggcagtcgag
     1921 ctgcaggatg aagccggcgt ccacgatcgc gcggtattcg cgcgccatga cgtcggcgag
     1981 cgcatagaga taggcttcgt cgctcgggt
//
LOCUS       DASYJA010000049         1835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2096146, whole genome shotgun sequence.
ACCESSION   DASYJA010000049 DASYJA010000000
VERSION     DASYJA010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2096146"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..426)
                     /locus_tag="VGO02_01080"
     CDS             complement(163..426)
                     /locus_tag="VGO02_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEV7476309.1"
                     /translation="MWCFLSAASAQDAQRLAAPCAICHGTDGRAATKEVLPLAGLPRD
                     YIVSQMRALRDGQRQATVMHQIAKGYTDAQIDALAAWFSTQKR"
     gene            complement(560..>1835)
                     /locus_tag="VGO02_01085"
     CDS             complement(560..>1835)
                     /locus_tag="VGO02_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7476310.1"
                     /translation="EKDHALSRRFQKIDVVEPSIDETVEILKGLKTRFEAHHSVKYTA
                     NALTTAAELSARYINDRHLPDKAIDVIDEAGAAQRIAPKAKQKKIIGKTEIEEIIAKI
                     ARIPPRSVSTDDRSALANLDRDLKAVVFGQDQAIDALAAAIKMARSGLGNPQKPIGNF
                     LFSGPTGVGKTEVARQLAYCMGIELIRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLL
                     TEAITKKPYSVLLLDEIEKAHPDIFNILLQVMDHGTLTDNNGRKADFRNVCIVMTTNA
                     GAESLAKNSMGFTLNKQMGDEMEAIKRMFTPEFRNRLDAIISFRALDTNTIMRVVDKF
                     LIQLEEQLAEKKVEALFTQALKDHLAKKGFDPLMGARPMARLIQDTIRRALADELLFG
                     KLQHGGKVTIDVDEAEKIVLKFEEQPAAVTTQ"
BASE COUNT          272 a          598 c          608 g          357 t
ORIGIN      
        1 cttggggtcc cacatgcgga tgtacttcgc cgcggtcgcc ccgccgaagc cgccgcccac
       61 caccaccacg cgcgctttgg acggtgcgtt ggccgcgcac ccggccaggc ccgccagaga
      121 gccggcggcc gcgaacccga ggaaatcgcg tcttttcatg ccttacctct tctgcgtgga
      181 gaaccacgcc gcgagcgcgt cgatctgcgc gtcggtgtac cccttggcga tctggtgcat
      241 caccgtcgcc tggcgctggc cgtcgcgcag cgcgcgcatc tgggagacga tgtagtcgcg
      301 cggcagcccg gcaagcggca acacctcctt ggtcgcggcg cgtccatcgg tgccatggca
      361 gatggcgcac ggcgccgcga ggcgctgcgc gtcctgtgcc gacgcggccg aaaggaaaca
      421 ccacatcagg acagcaaccg ccgttactcg tgtcatcggt cctcccgggg ggtgggagaa
      481 agtaacgccg ggtcccgaaa aaagcaaagg ccgccgaatg ttacattcgg gcggccttgc
      541 gcactgatgc ggctggcggc tactgggtgg tgacggccgc gggctgttcc tcgaacttga
      601 gcacgatctt ctcggcttcg tcgacgtcga tggtcacctt gccgccgtgc tgcagcttgc
      661 cgaacagcag ctcgtcggca agggcgcggc ggatcgtgtc ctggatcagg cgcgccatcg
      721 ggcgcgcgcc catcagcggg tcgaagccct tcttcgccag gtggtccttg agcgcctgcg
      781 tgaagagcgc ctcgaccttc ttctcggcga gctgctcctc gagctggatc aggaacttgt
      841 ccaccacgcg catgatggtg ttggtgtcga gggcgcggaa cgaaatgatc gcgtccagcc
      901 ggttgcggaa ctccggcgtg aacatgcgct tgatcgcctc catctcatcg cccatttgct
      961 tgttgagggt gaagcccatg ctgttcttgg cgagcgattc ggccccggcg ttggtcgtca
     1021 tgacgatgca gacgttgcgg aagtccgcct tcctgccgtt gttgtcggtc agggtgccgt
     1081 ggtccatcac ctgcagcagg atgttgaaga tgtccgggtg cgccttctcg atctcgtcca
     1141 gcagcaagac tgaatagggc ttcttggtga tcgcctcggt gagcaggccg ccctgctcga
     1201 agccgacata ccccggcggc gcgccgatca agcgcgacac ggcgtgccgc tccatgtact
     1261 cggacatgtc gaagcggatc agctcgatgc ccatgcagta ggcgagctgg cgcgccacct
     1321 cggtcttgcc gacgccggtg ggcccgctga acaggaagtt gccgatcggc ttctgcgggt
     1381 tgcccaggcc cgagcgcgcc atcttgatgg cggcggcgag cgcgtcgatc gcctggtcct
     1441 ggccgaaaac aaccgccttc agatccctat ccagatttgc cagggcgcta cggtcatccg
     1501 tagacacgct gcgcggcggg atgcgcgcga tcttcgcgat gatctcctcg atctcggtct
     1561 tgccgatgat cttcttctgc ttggccttgg gcgcgatgcg ctgcgcggcg ccggcttcgt
     1621 cgatgacgtc gatggccttg tccggcaggt ggcggtcgtt gatgtagcgc gccgagagct
     1681 ccgccgcggt ggtgagcgcg ttggcggtgt acttgacgct gtggtgcgcc tcgaagcgcg
     1741 tcttcaggcc cttcaggatc tccaccgtct cgtcgatcga gggctcgacc acgtcgatct
     1801 tctggaagcg ccgcgacaac gcatggtcct tctcg
//
LOCUS       DASYJA010000050         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_98618, whole genome shotgun sequence.
ACCESSION   DASYJA010000050 DASYJA010000000
VERSION     DASYJA010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98618"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2131)
                     /locus_tag="VGO02_01090"
     CDS             complement(<1..>2131)
                     /locus_tag="VGO02_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEV7476311.1"
                     /translation="KYFGGTTSNVGGMDVFDVAEEALRIHTAAFSGDPVLANSLDAGG
                     EYAWRVRGEEHMWTPDTIAKLQHATRKESYSTYKEYAQLINDQSARHMTFRGLFEFRR
                     ENVKPVPLEEVEPAAEIVKRFATGAMSLGSISTEAHTSLAVAMNRIGGKSNTGEGGED
                     RRRYAPVKAGETLQSRLTKSRIEVDFPIREGDSLKSKIKQVASGRFGVTAEYLASAEQ
                     IQIKMAQGAKPGEGGQLPGKKVSEYIAELRYSTPGVELISPPPHHDIYSIEDLAQLIH
                     DLKNANPKASISVKLVSEVGVGTVAAGVSKAKSDHVTISGHDGGTGASPWSSIKHAGT
                     PWELGLAETQQTLVLNRLRGRIAVQVDGQLKTGRDVVVGAILGADEFGFATAPLVVQG
                     CIMMRKCHLNTCPVGVATQDPVLRRKFSGKPEYVVNFFFFVAEEVRELMASLGVRKLS
                     ELIGRTDLLDTKKGIEHWKAKGLDFSRIFYMPKMPAEVALYNSEKQNHGLEKALDNRL
                     VELAQPALERGEKVSIDMPIRNINRTVGTMLSHEIAKRYGHEGLAHNTIHVRFAGSAG
                     QSFGAFLAKGVTLDLIGDTNDYCGKGLSGGRISVQPSPKFRGEPTENIITGNVVLYGA
                     IAGEAYFRGVAGERFAVRNSGAHAVVEGVGDLGCEYMTGGMVVVLGMTGRNFAAGMSG
                     GIAYVYDPGARFKDLCNTAMVELEPIGA"
BASE COUNT          315 a          713 c          701 g          402 t
ORIGIN      
        1 tgcgccgatc ggctcgagct cgaccatcgc ggtattgcac aggtccttga agcgcgcccc
       61 ggggtcgtag acgtaggcga tgccgcccga catgccggcg gcgaagttgc gcccggtcat
      121 gccgagcacc accaccatgc cgcccgtcat gtattcgcag ccgagatcgc cgacgccttc
      181 cacgaccgca tgcgcgccgg agttgcgcac cgcgaagcgc tccccggcga cgccgcggaa
      241 ataggcttcg ccggcgatcg cgccgtagag cacgacgttg ccggtgatga tgttctcggt
      301 cggctcgccg cggaacttgg gcgagggctg cactgagatc ctgccgccgg acaggccctt
      361 gccgcaatag tcgttggtgt cgccgatgag atcgagcgtc acgcccttcg ccaggaaggc
      421 gccgaagctc tgtcccgccg agccggcgaa gcgcacgtga atcgtgttgt gggcaagccc
      481 ttcgtggccg tagcgcttgg cgatctcgtg agacagcatc gtgccgaccg tgcggttgat
      541 gttgcggatc ggcatgtcga tcgacacctt ctcgccccgc tcgagcgccg gctgcgccag
      601 ctcgaccagc cggttgtcga gcgccttctc caggccgtgg ttctgcttct cgctgttgta
      661 gagcgcgacc tcggcgggca tcttcggcat gtagaagatg cgcgagaagt ccaggccctt
      721 cgccttccag tgctcgatgc ccttcttcgt gtcgagcagg tcggtgcggc cgatcagctc
      781 ggagagcttg cgcacaccga ggctcgccat cagctcgcgc acttcctcgg cgacgaagaa
      841 gaagaagttc accacgtatt cgggcttgcc cgagaacttc cggcgcagca ccggatcctg
      901 cgtcgccaca cccaccgggc aggtgttgag gtggcacttg cgcatcatga tgcagccctg
      961 caccaccagc ggcgcggtgg cgaagccgaa ctcgtcggcg ccgaggatcg cgccgaccac
     1021 cacgtcgcgc ccggtcttca gctggccgtc gacctgcacg gcaatcctgc ctctcaatct
     1081 gttcaaaacc agtgtttgct gcgtctccgc gagaccgagc tcccaggggg tgccggcgtg
     1141 cttgatcgag ctccacggcg aggcgccggt gccgccgtcg tgtccggaga tggtgacgtg
     1201 gtcggacttg gccttggaga cgcccgccgc gaccgtgccg acgccgactt cggacaccag
     1261 cttcaccgag atggaggcct tcgggttggc gtttttcagg tcgtggatca gctgcgccag
     1321 atcctcgatc gagtagatgt cgtggtgcgg cggcgggctg atcaactcga cgccgggcgt
     1381 cgagtagcgc agctcggcga tgtactcgga caccttcttg ccgggcagct gcccgccctc
     1441 gccgggcttg gcgccctgcg ccatcttgat ctggatctgc tccgcgctcg ccaggtattc
     1501 cgcggtgacg ccgaagcgac ccgaggcgac ctgcttgatc ttcgacttca gtgaatcacc
     1561 ctcgcgtatc gggaaatcca cttcaattct tgattttgtt aaacgggact gaagcgtttc
     1621 ccccgccttg acgggcgcgt agcggcggcg gtcctcgccg ccctcgccgg tgttcgactt
     1681 gccgccgatg cggttcatcg ccacggcgag cgaggtgtgc gcctcggtcg agatcgagcc
     1741 gagcgacatg gcgccggtgg cgaagcgctt gacgatctcg gcggcgggct cgacttcctc
     1801 cagcggcacc ggcttcacat tctcgcggcg gaactcgaac aggccgcgga aggtcatgtg
     1861 gcgcgccgac tggtcgttga tgagctgcgc gtactccttg taggtcgagt aggactcctt
     1921 gcgcgtcgcg tgctgcagct tggcgatcgt gtcgggcgtc cacatgtgtt cctcgccacg
     1981 cacgcgccag gcgtattcgc cgcccgcgtc caggctgttg gccagcaccg ggtcgccgct
     2041 gaaggcggcg gtgtggatgc gcagcgcttc ctcggccacg tcgaacacgt ccatgccgcc
     2101 cacgttcgag gtggtgccgc cgaagtactt g
//
LOCUS       DASYJA010000051         5841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1732979, whole genome shotgun sequence.
ACCESSION   DASYJA010000051 DASYJA010000000
VERSION     DASYJA010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732979"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VGO02_01095"
     CDS             <1..876
                     /locus_tag="VGO02_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEV7476312.1"
                     /translation="RVITDLFEPGSTLKPFTIALALETGKVTPATMVHTAPGRLTIAN
                     YTIHDVHPAAALSLSQVVQKSSNVGAARLALAMPSEAMWDMFQRVGFGAPPQLGFPGA
                     AAGRLRPYKTWRPIEQATMAYGHGISVSLAQLARAYTVFARDGELVPLTLVKTNAEAV
                     GEKVLSAASAKAVRAMLELAVQPGGTGPRARIMGWRVAGKTGTAHKQENGGYAVDKYL
                     ASFAGFAPVSAPRLVVAVMIDEPSAGQHYGGSVAAPVFAHVMQGALRLLGVPHDAPLE
                     PLELPRAGDEAGEST"
     gene            887..2296
                     /locus_tag="VGO02_01100"
     CDS             887..2296
                     /locus_tag="VGO02_01100"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEV7476313.1"
                     /translation="MSTSASGFLRQLAAQGAMIERLASDSRRCAPGVAFLAYPGEAAD
                     GRAHIDDAIRRGAAAVLWEHTGFTWREEWRVPNAAVPGLKQHAGELAAEFYGRPSESL
                     WMCGVTGTNGKTSCSQWIAAALNRKGIYSNVIGTLGYGTPGALQPLPNTTPDALEVQA
                     LLKKFVDEKSEAVAMEVSSHGLAQGRVNGVAFDCALFTNLSHDHLDYHGTMQAYAAAK
                     EKLFDTPGLCAAVLNFDDAMGVRLARRLRGKVRVIGYGFADTAAAAADEYLPAKLLPP
                     EHTRQLGRFNLANALGVLGCLLAYGLERKEAAVLLADLPPVPGRMQRIAERPLVVVDY
                     AHTPDALEKVLQALRPVTQERRGKLAVVFGAGGDRDPTKRPLMGAVATRLADKVLVTS
                     DNPRSEDPLEIIRQIGAAASEPDRARAIEKIIAEAASSDVVLIAGKGHEDYQEIAGRL
                     LPFSDAAVARAAISKRSSA"
     gene            2293..3642
                     /gene="murF"
                     /locus_tag="VGO02_01105"
     CDS             2293..3642
                     /gene="murF"
                     /locus_tag="VGO02_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEV7476314.1"
                     /translation="MMSLADAAAMTAGRTSGGNPVFTGVSTDTRTLRSGDLFVALRGE
                     RFDGHGFLAQARSGGASAAMVDSRHQGEFPLPVVVVEDTKRSLGDLARLWRARFSPAL
                     IAIAGSNGKTTVKEMLASILRSHAGEDAVLATAGNLNNDIGLPLTLLRLRAAHRCCAI
                     ELGMNHKGEIAYLAGIAAPTVALVNNAQREHLEFMNSVEEVAAENAGVYAALPAAGVA
                     VINADDAHADLFRSRAGERRVVDFGLEAAAVVTGRHVLRPLTSEISIRTPAATAQATL
                     AIPGLHNVRNALAAAACAFAVGIKEKTIGEGLSAFRPYTGRLQVKLARGGATVIDDSY
                     NANPDSVRAAIDVLASCPGPTALVLGDMGEVGSQGAEFHREVGSYASSRNISRLYAIG
                     DATRYAVEAFGAGGRHFDSVDELVSSIEGRSILVKGSRFMRMERVVAALTGSATGGH"
     gene            3642..4733
                     /gene="mraY"
                     /locus_tag="VGO02_01110"
     CDS             3642..4733
                     /gene="mraY"
                     /locus_tag="VGO02_01110"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV7476315.1"
                     /translation="MLLEIAQWLAKDVRLFNVFNYITLRAVLACLTALAVSLILGPYV
                     IRKLTAYKIGQAVRDDGPRSHLTKAGTPTMGGALILVSIVVTTLLWGDLTNRLVWVVL
                     LVTLAFGTVGWIDDYRKVVHRNPKGLPAKAKFFWQALIGLAAACYLAYVATSMPALNQ
                     LIVPFFKFVAYPLGTFGFIVLTFFVIVGTSNAVNLTDGLDGLAIMPTVMVGSALGIFA
                     YVAGNAVFSKYLAVPYVPGAGELAVICGALAGAGLGFLWFNAYPAEVFMGDVGALALG
                     AALGTIAVIVRQEIVLFIMGGVFVAETLSVMLQVLYFKWTGGKRIFRMAPLHHHYELE
                     GWKESQVVVRFWIITIMLVLFGLSTLKLR"
     gene            4733..>5841
                     /gene="murD"
                     /locus_tag="VGO02_01115"
     CDS             4733..>5841
                     /gene="murD"
                     /locus_tag="VGO02_01115"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV7476316.1"
                     /translation="MREPAFSLLPRPAPMPLKGKRVLVLGLGDTGLSVARWVESEGGK
                     ARVADTREAPPRRKDFSGELHTGPFSDALLDGADLLCISPGLPLHDPLVQIAIERKIP
                     VVGDIELFAWRNTSKVLAITGTNGKTTVTSLTGHLLKSAGFDCEVAGNIAPPVLQAAL
                     QRKTPPAAWVLELSSYQLETTWSLKPHAAAMLNLTEDHLDRYAGIAEYGAAKARIFAG
                     SGVQVLNRDDPRSLAMLLPGRKVITFGLDGDGDYGVRGDTLICRGVPMLRLDELQIHG
                     GHNVANALAACALAGSLGASTSSLAVGLRSFKGLPHRLERIASLRGVEWYDDSKGTNV
                     GATVAALRGLGKRVVLIAGGEGKGQDFTPLAEPAR"
BASE COUNT          914 a         1981 c         2020 g          926 t
ORIGIN      
        1 cgggtgatca cggacctctt cgagccgggc tcgaccctca agcctttcac gatcgcgctc
       61 gcgctggaga ccggcaaggt gacgccggcg accatggttc acaccgcacc cggtcgtctc
      121 acgatcgcga actacacgat ccacgacgtg catccggcgg cggcgctcag cctgtcgcag
      181 gtggtccaga agtcgtccaa cgtcggcgcg gcgaggctcg ccctcgccat gccgagcgag
      241 gcgatgtggg acatgttcca gcgcgtcggt ttcggcgccc cgccgcagct cggctttccg
      301 ggggccgctg cgggccggct gcgcccctac aagacctggc ggccgatcga gcaggcgacc
      361 atggcctacg gccacggcat ctccgtcagc ctggcgcagc tcgcgcgcgc ctacaccgtg
      421 ttcgcgcgcg atggggaatt ggtgccgctc acgctggtca aaacgaatgc cgaggcggtc
      481 ggcgagaagg tgctgtccgc cgcatcggcc aaggcggtgc gcgccatgct cgagctcgcc
      541 gtgcagcccg gcggcacggg gccgcgcgcg cggatcatgg gttggcgcgt ggcgggcaag
      601 accggcaccg cgcacaagca ggaaaacggc ggctatgccg tggacaagta ccttgcctcc
      661 ttcgccggct tcgcgccggt ttcggcgccg cgcctggtgg tggcggtgat gatcgacgag
      721 ccctcggccg ggcagcacta cggcggctcg gtcgcggcgc cggtattcgc gcacgtgatg
      781 cagggcgcgc tgcgcctgct cggcgtgccg cacgacgcgc cgctcgagcc gctcgagctg
      841 ccgcgcgccg gcgacgaggc cggggagagc acttgacggg cgagccatgt cgacgagcgc
      901 atccggattc ctgcggcagc tggccgcgca aggagcgatg atcgagcggc ttgcgtcgga
      961 cagccgccgc tgcgcgccgg gcgtggcctt tctcgcctat ccgggtgaag cggcggacgg
     1021 ccgcgcgcat atcgacgatg cgatccgccg cggcgccgcg gccgtgctgt gggaacacac
     1081 cggcttcaca tggcgcgagg agtggcgcgt gccgaacgcc gcagtgcccg gcctcaagca
     1141 gcacgccggc gagctggcgg ccgagttcta cggccggcct tccgaatcgc tctggatgtg
     1201 cggcgtgacc gggaccaacg gcaagacttc gtgcagccag tggatcgccg ctgctctcaa
     1261 caggaaaggc atttacagca acgtgatcgg tactctcggc tacggtaccc ccggtgccct
     1321 ccagcccttg ccgaacacga cgccggatgc gcttgaggtt caagcgctgc tcaagaaatt
     1381 cgtggatgaa aagagcgagg cggttgcaat ggaggtgtcg tcgcacggtc tcgctcaggg
     1441 ccgggtcaac ggcgtggcgt tcgattgcgc gctattcacc aacctctcgc acgaccacct
     1501 cgactatcac ggcacgatgc aggcctacgc ggcggcgaag gagaagctgt tcgacacgcc
     1561 ggggctgtgc gctgcagtgc tcaatttcga cgacgcgatg ggagtacgcc tcgcgcggcg
     1621 cttgcggggg aaggtgcgcg tgatcggcta tggattcgcc gataccgccg ccgccgcggc
     1681 cgacgaatac cttccggcca agctgctgcc gccggaacac acgcggcagc tggggcgctt
     1741 caatctcgcc aatgcgcttg gcgtgctcgg ctgcttgctg gcgtacgggc tggagcggaa
     1801 ggaggcggcg gtgctgctcg ccgatctgcc gccggtgcca ggccgcatgc agagaatcgc
     1861 cgagcggccg ctggtcgttg tggactatgc gcacacgccg gacgcgctgg agaaagtgct
     1921 gcaagctctg cgtccggtga cgcaggagcg ccgcggcaag ctcgccgtgg tgttcggcgc
     1981 gggcggcgac cgcgatccga ccaagcggcc gctgatggga gcggtcgcga cacggctcgc
     2041 cgacaaggtc ctggtcacct cggacaaccc gcgcagcgag gaccccctgg aaatcatccg
     2101 ccagatcgga gccgccgcca gcgaaccgga ccgcgcccgt gccatcgaga aaatcatcgc
     2161 ggaagcggcg agcagcgacg tagtcctgat cgccggcaag ggccatgagg actaccagga
     2221 gatcgccggc cgcctcctgc cgttctccga cgcagccgtg gcgcgcgcag ccatcagcaa
     2281 gcggagcagc gcatgatgag cctcgccgac gccgcggcaa tgactgcagg acgcaccagc
     2341 ggcggcaacc cggtgtttac cggcgtgtcg accgacacgc gcacgctgcg ctcgggcgac
     2401 ctgttcgttg cgctgcgcgg cgagcgtttc gacggccacg gctttctcgc ccaggcgagg
     2461 agcggcgggg cgagcgcggc gatggtcgac agccgccacc agggcgaatt tccgctgccg
     2521 gtagtcgtgg tggaggatac caagcgctcg ctcggcgatc tggcgcgcct gtggcgcgcg
     2581 cgcttttcgc cggccctgat cgcgatcgcc ggctcgaacg gcaagaccac ggtgaaggaa
     2641 atgctggcct cgatcctgcg cagccacgcg ggcgaggacg cggtgctggc cactgccggc
     2701 aacctgaaca acgacatcgg gctgccgctc acgctgctgc gcctgcgcgc ggcgcaccgc
     2761 tgctgcgcga tcgagctcgg catgaaccac aagggcgaga tcgcatacct cgccgggatt
     2821 gccgcgccga cggtggcgct ggtgaacaac gcccagcgcg agcatctcga gttcatgaac
     2881 tcggtcgagg aggtggcggc ggagaacgca ggcgtctacg cggcgctgcc cgccgcgggc
     2941 gtcgcggtta tcaacgccga cgacgcgcac gccgatctct tccgcagccg tgccggcgag
     3001 cgccgcgtcg tcgacttcgg cctggaagcg gccgccgtcg tgaccggccg ccacgtgctc
     3061 aggccgctga ccagcgaaat ctccatccgt acgcccgccg caaccgcgca ggcgacgctg
     3121 gcgattcccg gcctgcacaa cgtgcgcaat gccctggctg cggccgcgtg tgcttttgca
     3181 gttggaatca aagaaaaaac aatcggtgaa gggctgagcg ctttccgccc ctacaccggg
     3241 cgcctgcagg tcaagctggc gaggggcggg gcgacggtga tcgacgatag ctacaacgcc
     3301 aatccggatt cggtgcgcgc ggcgatcgat gtgctcgcct cctgcccggg tccgaccgcc
     3361 ctggtgctcg gcgacatggg cgaagtcggc tcgcagggcg ccgaattcca ccgcgaagtg
     3421 ggttcttatg cttcatcaag aaacatttcc aggctgtacg cgatcggcga tgcgacgagg
     3481 tatgccgtcg aagcgtttgg cgcgggaggc cgccacttcg attccgtcga tgagctggtt
     3541 tcgagcatcg agggccgcag catcctcgta aaaggctcgc gcttcatgcg catggagcgc
     3601 gtcgtagcgg cgctcaccgg cagcgccacg ggaggtcact gatgctgctc gagatcgcgc
     3661 aatggctggc caaggacgtg cggctgttca acgtcttcaa ctacatcacg ctgcgcgcgg
     3721 tgctcgcctg cctgaccgcg ctcgcggtgt cgctgatcct cgggccctac gtcatccgca
     3781 agctcaccgc ctacaagatc ggccaggcgg tgcgcgacga cggcccgcgc tcgcacctca
     3841 ccaaggccgg cacgccgacg atgggcggcg cgctgatcct ggtgtcgatc gtggtcacca
     3901 cgctgctgtg gggcgacctg acgaaccgcc tggtgtgggt ggtgctgctc gtgacgctcg
     3961 ccttcggcac cgtgggctgg atcgacgact accgcaaggt ggtgcaccgc aatccgaagg
     4021 ggctcccggc caaggccaag ttcttctggc aggcgctgat cggcctcgcc gccgcctgct
     4081 atctcgccta tgtcgcgacc agcatgcccg cgctcaacca gctgatcgtg ccgttcttca
     4141 agttcgtcgc ctatccgctg ggcacgttcg gcttcatcgt gctcaccttc ttcgtcatcg
     4201 tcggcacctc caacgcggtc aacctgaccg acggactgga cggcctcgcc atcatgccga
     4261 cggtcatggt gggcagcgca ctgggcatct tcgcctacgt cgcgggcaac gcggtgttct
     4321 cgaaatacct cgccgtgccc tacgtgccgg gtgcgggtga gctcgcggtc atctgcggcg
     4381 cgctcgccgg ggccggcctc ggcttcctct ggttcaacgc ctatccggcc gaggtgttca
     4441 tgggcgacgt cggtgcgctc gcgctcggcg cggcgctcgg caccatcgcc gtgatcgtgc
     4501 gccaggagat cgtgctgttc atcatgggcg gcgtattcgt cgccgagacg ctgtcggtga
     4561 tgctgcaggt gctgtacttc aagtggaccg gcggcaagcg catcttccgc atggcgccgc
     4621 tgcaccacca ctacgagctc gagggctgga aggagagcca ggtggtggtg cgcttctgga
     4681 tcatcaccat catgctggtg ctgttcggcc tgtcgacgct caagctcaga tgatgcgcga
     4741 gcctgccttc tcgctcctgc cgagaccggc gcccatgccg ctgaagggca agcgggtcct
     4801 cgtgctcggc ctcggcgaca cgggcctctc ggtcgcgcgc tgggtcgagt ccgagggcgg
     4861 caaggcgcgc gttgccgata cgcgcgaggc gccgccgcgg cgcaaggatt tctccggcga
     4921 gctgcatacc ggacctttct cggacgccct gctggatggc gcggacctgc tgtgcatcag
     4981 ccccggcctg ccgcttcacg atccgctggt tcaaattgca atcgaaagaa aaattccggt
     5041 cgtcggcgac atcgagctgt tcgcctggcg caacacgagc aaggtgctcg cgataaccgg
     5101 caccaacggc aagacgacgg tcacctcgct caccggccac ctgctgaaga gcgccgggtt
     5161 cgattgcgaa gtggcgggca acatcgcccc gcccgtgctg caagccgccc tgcagcggaa
     5221 aactccgccc gcggcgtggg tgctcgagct atccagctac cagctggaaa cgacctggtc
     5281 cttgaagccg cacgcggcgg cgatgctgaa cctgaccgag gaccatctcg accgctacgc
     5341 cgggatcgcc gagtacggcg cggcaaaggc gcgcatcttc gccggttccg gcgtccaggt
     5401 gctgaaccgg gacgacccgc gctcgctcgc catgctgctc ccgggaagga aggtgatcac
     5461 cttcgggctc gacggagacg gggactatgg cgtacggggg gatactctca tctgcagggg
     5521 cgttcccatg ctgcgcctcg acgagttgca gatccacggc ggccacaatg tcgccaacgc
     5581 gctggctgct tgcgccctcg ccggcagcct cggagcttcg accagcagcc tggcggtcgg
     5641 cctgcgcagc ttcaagggcc tgccgcaccg gctggagcgc atcgcatcgt tacgcggcgt
     5701 cgagtggtac gacgactcga aggggacgaa cgtcggcgcg accgtcgccg cgctgcgcgg
     5761 cttgggcaag cgggtcgtgc tgatcgccgg cggcgagggc aaggggcagg acttcacgcc
     5821 gctcgccgag ccggcgcgcg a
//
LOCUS       DASYJA010000052         1561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_825982, whole genome shotgun sequence.
ACCESSION   DASYJA010000052 DASYJA010000000
VERSION     DASYJA010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825982"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1062)
                     /locus_tag="VGO02_01120"
     CDS             complement(106..1062)
                     /locus_tag="VGO02_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV7476317.1"
                     /translation="MPRHRDYVPQGVIPAVLLPFHDDLSIDEAAYRAHLRDVASVGGL
                     EAITTNAHASEVASCTFEEQRRILEITQEEIGARLPIVNGIYAEGSLEAQRLAGMAAA
                     GGASALLVFPPGVYTFGQRPEMALEHFRRIADVTDLPLIVFQYPLAGGQGYPMSTLER
                     ICDELPTVRAIKDWCASPLLHERHIRALQSRARPVNVLTTHSSWLLSSLVLGCKGLLS
                     GSGSVIADLQAQLWRAVQANDLATAKAIHERIYPTAEAFYAEPFVDMHNRMKEALVLL
                     GRMPRAVVRPPLVKLPADEIERIREALVAAGLLDREGRRKAA"
     gene            1360..>1561
                     /locus_tag="VGO02_01125"
     CDS             1360..>1561
                     /locus_tag="VGO02_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476318.1"
                     /translation="MRFASFLLLFGLSLAARAQGDEFGVRYWYSQATTTRSHNAQTVF
                     PSLGNPTSTLTYENLTAHALEMH"
BASE COUNT          269 a          513 c          526 g          253 t
ORIGIN      
        1 gaccgcccgg cgggaacggc acaatgagcc gcaccgggcg ggcaggaaaa tctgctgccc
       61 aaaccggggt tatgcaaaca acaacaatga aagcgaaaag ccggatcatg cggccttccg
      121 tcttccctcc cgatcgagca aacccgcagc gacgagcgct tcgcggatgc gctcgatttc
      181 gtccgccggc agcttgacga gcggcggacg cactacggcc cgcggcatcc ggccgagcag
      241 cacgagcgct tccttcatgc ggttgtgcat gtcgacgaag ggctcggcgt agaacgcttc
      301 ggccgtcgga tagatgcgct cgtggatcgc tttcgcggtg gccagatcgt tcgcctggac
      361 cgcgcgccag agctgcgcct gcaggtcggc gatcaccgag ccgctgccgg aaagaagccc
      421 cttgcagccg agcacgagcg agctgaggag ccaggagcta tgcgtggtga gcacgttcac
      481 cggccgcgcg cgcgattgca gcgcgcggat gtgccgctcg tgcagcagcg ggctcgcgca
      541 ccagtccttg atcgcgcgca ccgtgggcag ttcgtcgcag atgcgctcga gggtgctcat
      601 gggatagccc tggccgcccg cgagcggata ctgaaacacg atgagcggca ggtcggtgac
      661 gtcggcgatg cgccgaaagt gctcgagcgc catctcgggg cgctgcccaa acgtgtagac
      721 gcccggcggg aagacgagca gcgccgaagc gcctcccgcg gcggccatgc ctgcgagacg
      781 ctgcgcttcg agcgagccct cggcgtagat gccgttcacg atcggcaggc gcgcgccgat
      841 ctcctcctgc gtgatctcga ggatccggcg ctgctcctcg aaggtgcacg aggccacctc
      901 ggaggcgtgc gcgttcgtgg tgatcgcctc gagccccccg acggaggcca cgtcgcgcag
      961 gtgcgcgcgg taagccgctt cgtcgatcga caggtcgtcg tggaagggca acagcaccgc
     1021 ggggatgacc ccctgcggaa cgtagtcgcg atggcgtggc atgggcgtcc tcctcggggc
     1081 gcgagggcta ttctagccgc ctgaaaaaac aaacgccccg aggcttgcgc ccggggcgtc
     1141 tgctaggcag ccggcctaga tgcgggccgg actcctgttc ttaggattgc ccccaaactg
     1201 cacgggtcga cgatccacca ttagagtcct gccggccggt cgtgccttgg tcgtctctca
     1261 cgggatcatc tccttttcaa agttgccagc cttcagttta gcgtggaacg cacgcggcgc
     1321 aaaacggcgg caatggccgg ctgggctaga ctgccacgca tgcgttttgc ttcgttcctg
     1381 ctgcttttcg gcctgagcct cgcggcgcgg gctcagggcg acgagttcgg cgtgcgctat
     1441 tggtacagcc aggcgacgac gacccgaagc cacaatgcgc agacggtgtt tccgtcgctc
     1501 ggcaacccga cttcgaccct gacctacgaa aaccttacgg cgcacgccct ggagatgcac
     1561 g
//
LOCUS       DASYJA010000053         3184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2731692, whole genome shotgun sequence.
ACCESSION   DASYJA010000053 DASYJA010000000
VERSION     DASYJA010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2731692"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..422)
                     /locus_tag="VGO02_01130"
     CDS             complement(135..422)
                     /locus_tag="VGO02_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476319.1"
                     /translation="MKTLILAAALLQPAVAPNAPPLYSFADVYRLTVNGAAMADFPAA
                     AGALRVAVTPQPQPEAGFSVRAMPAPEGWLLVFSGLAAAFWVARRRLGYSY"
     gene            complement(570..1424)
                     /gene="epsA"
                     /locus_tag="VGO02_01135"
     CDS             complement(570..1424)
                     /gene="epsA"
                     /locus_tag="VGO02_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtB/PEP-CTERM-associated transcriptional
                     regulator EpsA"
                     /protein_id="HEV7476320.1"
                     /translation="MQPTTSSDISEPSILVPQPASQYGGDGEAHTSAADFLQIESIVL
                     NLDASLRVHARAHFFSWTQGLLQSLIRHELLICTLCLGKPPAFRADGFSMTVAEPAAL
                     SDVFMRDTAVAPALLKAWEERRFQPVTLDVSNSSFGNGAFGRELERLSATQLLVHGVH
                     DADGRALSLFTFACRPGSAGPRQSYLVQLLAPSMHAAWVRTQLQRRAEGNGAGERPGG
                     GSILTVRELDILKWIYLGKSNYEIGAILKISPLTVKNHVQKILRKLNVVNRTQAIGKS
                     LELRLLNP"
     gene            1561..2961
                     /locus_tag="VGO02_01140"
     CDS             1561..2961
                     /locus_tag="VGO02_01140"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEV7476321.1"
                     /translation="MSSLPETRARFTRGQPGAAALLQAAVDPLVAIAMLAAAVAAFGD
                     SFDGGCLILSLLVFAMTFPGSLARASAPSAVELALDIAAGWFAVVGLLLLLGWASHTL
                     GAFDERAMLAWALATPAALFAAHRALPLWLPRILSAEGLHKTAVIAGANDLGRRLAAR
                     LRGDPMLGVRFAGYFDDRGAVRLRDLAAAENLGTLERLADYVRANPVDVIYIALPMAS
                     QPRILRLLEDLRDTTISIYFVPDIFVSDLIQARVDFIGELPVVAVCETPFYGFNGLVK
                     RVSDFVLAGTILLGISPLMAAIAIGVKLSSPGPVLFKQRRYGVDGRKIVVYKFRSMTV
                     AEDGDVVRQATRNDSRITKFGAFLRRTSLDELPQFINVLQGRMSVVGPRPHAVAHNEL
                     YRKLIRGYMIRHKVRPGITGLAQVNGMRGETDTVDKMKLRIEYDLAYLRNWSLALDLK
                     IVLKTVAVVLKRQNAY"
     gene            2951..>3184
                     /locus_tag="VGO02_01145"
     CDS             2951..>3184
                     /locus_tag="VGO02_01145"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV7476322.1"
                     /translation="MRTEQRFSLLCALVLVAQLFTLSSIPFEIHEPWDMVWHFLAYSA
                     LTLLLWIGTDGRRPVAVVALVMVLGAMDELRQSF"
BASE COUNT          473 a         1099 c         1086 g          526 t
ORIGIN      
        1 tgctcgagcg cctgccggct ggacgcctcg ccggccagga tctcggcgcc gttgaccttg
       61 gccagcaccg gatgcgtctc cagccgctgg caggcggtca acgacactat tagtagaagg
      121 aaaacgcagc gcatctagta ggagtacccg aggcgccgcc gcgccaccca gaaggcggcc
      181 gccaggccgg agaagaccag cagccagccc tcgggggccg gcatggcgcg caccgagaac
      241 ccggcctcgg gctgcggctg cggcgtcacc gctacgcgca gcgcgccggc ggcggccggg
      301 aagtcggcca tcgcggcgcc gttgacggtg aggcgataga cgtcggcgaa cgaatagagc
      361 ggcggagcgt tgggcgcgac cgccggctga agcagcgcag ccgcgaggat gagagtcttc
      421 atggaggaga cagatgctag aaggggtctg ttacgacgct cgggcgcgac gcgccggagt
      481 tcatgcaccg tacggactat cacgtcactg cccgcaccaa aaaaaatcgg ggccgggccc
      541 cgatttttcc gacggcgtga aattacttcc tagggattga gcaggcgcag ctcgagcgac
      601 ttgccgatgg cctgcgtgcg gttcaccacg ttcagcttgc gcaggatctt ctgcacgtgg
      661 ttcttcaccg tgagcgggct gatcttgagg atggcgccga tctcgtagtt gctcttgccg
      721 aggtagatcc acttcaggat gtcgagctcg cgcaccgtga ggatgctgcc gccgccgggc
      781 cgctcgccgg cgccgttgcc ctcggcgcgg cgctgcagct gcgtgcgcac ccacgccgcg
      841 tgcatcgacg gggcgagcag ctgcactaag tagctctggc gcggcccggc cgagcccggc
      901 cggcaggcga aggtgaacag cgacagcgcg cggccgtcgg cgtcgtgcac gccgtgcacc
      961 aggagctgtg tcgcgctcag gcgctccagt tcgcggccaa acgctccatt tccaaaactt
     1021 gaatttgaaa catccagcgt caccggctgg aagcgccttt cctcccacgc cttgagcagc
     1081 gccggcgcga cggcggtgtc gcgcatgaag acgtcgctca gcgcggccgg ctcggccacc
     1141 gtcatcgaga agccgtcggc gcggaacgcc ggcggcttgc ccaggcacag cgtgcagatc
     1201 agcagctcgt ggcggatcag gctttgcagc agcccttgcg tccagctgaa gaagtgcgcg
     1261 cgcgcgtgca cgcgcagcga ggcgtcgagg ttgagcacga tcgactcgat ctgcaggaag
     1321 tcggccgcgg aggtatgggc ctcgccatcg ccaccgtact gcgacgccgg ctgcgggacg
     1381 aggatggacg gttcgctgat gtcgcttgag gtggttggtt gcatggctcg ctcaatataa
     1441 gtgggggcaa taggcaatag ttcgctcggc gcaggggccc aaagctagtc cgatgggctt
     1501 atcgcggcgc tccgcacgca acgtcgtggc ggaaccgcgt gcgcctaatc tctttcgtcc
     1561 ttgtcttctc tccccgaaac tcgtgcgcgc ttcacccgcg gccagcccgg cgcggcggcg
     1621 ctgctgcagg cggcggtcga tccgctggtg gcgatcgcca tgctcgccgc ggcggtggcc
     1681 gccttcggcg acagcttcga cggcggctgc ctgatccttt cgctgctggt cttcgccatg
     1741 accttccccg gcagcctggc gcgcgccagc gcgccgagcg ccgtcgagct cgcgctcgac
     1801 atcgccgccg gctggttcgc ggtggtcggc ctgctgctgc tgctcggctg ggcctcgcat
     1861 accctgggcg ccttcgacga gcgcgccatg ctcgcctggg cgctcgccac gcccgcggcc
     1921 ctgttcgccg cgcaccgcgc gctgccgctg tggctgccgc gcatcctgtc ggccgagggc
     1981 ctgcacaaga ccgcggtgat cgccggcgcc aacgacctgg gccggcgcct cgccgcgcgc
     2041 ctgcgcggcg acccgatgct cggcgtgcgc ttcgccgggt acttcgacga ccgcggcgcg
     2101 gtgcgcctgc gcgacctggc ggccgccgag aacctcggca cgctcgagcg cctcgccgac
     2161 tacgtgcgcg ccaacccggt cgacgtcatc tacatcgccc tgccgatggc ctcgcagccg
     2221 cgcatcctgc gcctgctcga ggacctgcgc gacaccacca tctcgatcta cttcgtgccc
     2281 gacatcttcg tctccgacct gatccaggcg cgcgtcgact tcatcggcga gctgccggtg
     2341 gtggcggtgt gcgagacgcc gttctacggc ttcaacggcc tggtgaagcg ggtcagcgat
     2401 tttgtcctcg ccggcaccat cctgctgggc atttccccgc tcatggcggc gatcgcgatc
     2461 ggcgtcaagc tgtcgtcgcc cgggccggtg ctgttcaagc agcgccgcta cggcgtcgac
     2521 ggccgcaaga tcgtggtcta caagttccgc tcgatgacgg tggccgagga cggcgacgtg
     2581 gtgcgccagg cgacgcgcaa cgactcgcgc atcaccaagt tcggcgcatt tctgcgccgc
     2641 acctccctgg acgagctgcc gcagttcatc aacgtgctgc aggggcgcat gagcgtggtc
     2701 gggccgcggc cgcacgccgt ggcgcacaac gagctgtacc gcaagctgat ccgcggctac
     2761 atgatccgcc acaaggtgcg cccgggaatt accggcctgg cccaggtgaa cggcatgcgc
     2821 ggcgagaccg acaccgtcga caagatgaag ctgcgcatcg agtacgacct cgcctacctg
     2881 cgcaactggt cgctcgcgct cgacctgaag atcgtcctca agaccgtcgc cgtcgtgctg
     2941 aagaggcaga atgcgtactg aacagaggtt ctccctgctc tgcgcgctgg tgctggtggc
     3001 gcagctcttc accctcagct cgatcccgtt cgagatccac gagccgtggg acatggtgtg
     3061 gcacttcctc gcgtactcgg cgctgaccct gctgctgtgg atcggcaccg acggccgccg
     3121 gccggtcgcg gtggtggcgc tggtcatggt gctcggcgcc atggacgagc tgcgccagtc
     3181 gttc
//
LOCUS       DASYJA010000054         2348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1733258, whole genome shotgun sequence.
ACCESSION   DASYJA010000054 DASYJA010000000
VERSION     DASYJA010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733258"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VGO02_01150"
     CDS             <1..689
                     /locus_tag="VGO02_01150"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEV7476323.1"
                     /translation="DKMWDQPTGAFTSSYLLTEGGKHYSHAKRHGTGLSAVSFRIDNY
                     SDVEGKVGKAPAEQLLARIAKLVQETLRAEDSVGRMGPALFSAILPSTAAEQAVVFAR
                     RLRERLDSARITHGTEAIRIRTSFGVAAMGHDNPGSIEDLLKIAAERLEKAAAKSEEQ
                     RAAAPNDFGATLQRGIGNDVELAIKMLDKAAVERASEVLEKLGPLVKTTCARVGIDLQ
                     EFLFLLQSKP"
     gene            complement(696..1265)
                     /locus_tag="VGO02_01155"
     CDS             complement(696..1265)
                     /locus_tag="VGO02_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEV7476324.1"
                     /translation="MRAALHWVLLLALAGCASAPTSDEYAETIALFRGAGQSAKFFGD
                     SYGYAVFPTIGKGGLVVGGAHGLGRVYAKGAHVGDATVTQLTVGLQLGGQAYSQIIFF
                     QDKRAFDEFTTGEFEFGAQATAVAITAGASAQATTGGTSAGASSAGAPGHASTAGSYQ
                     KGMAVFTVAKGGLMYEASLGGQKFGYKPK"
     gene            complement(1268..1891)
                     /locus_tag="VGO02_01160"
     CDS             complement(1268..1891)
                     /locus_tag="VGO02_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476325.1"
                     /translation="MNYGRSVLVAALAAAAGSAQAAGAGLRIGTTGLGGDLGWDIAPT
                     LGGRVGLSAGSYSTDVKTADVNYDAKLKLGNLNAFLDWSPFGPFRISAGVIANNNKVD
                     VNGQANLDSRVAVNGTVKPGHSVAPYLGIGYGNVWTKGVNFYFDLGVMFQGSPNASLS
                     VNCGSATGTPECTRAQAQTAAEEQRLEDKLKNFKYFPVANIGITIGF"
     gene            complement(1925..>2348)
                     /locus_tag="VGO02_01165"
     CDS             complement(1925..>2348)
                     /locus_tag="VGO02_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476326.1"
                     /translation="PSLPGSHIATFRRWLLRIHGTVLALVALGSAVATTAGWKTGSGL
                     FGFMQQNPMVWVGLIQAYLLMAIIAVMLVLGSGQANARKWNVVGALAHCPALIAALSS
                     LEVFESMGAIGIVRAAIAFHVAWLCLETFTALYPDPKI"
BASE COUNT          414 a          823 c          740 g          371 t
ORIGIN      
        1 acgacaagat gtgggaccag cccaccggcg cgttcacctc gagctacctg ctcaccgagg
       61 gcggcaagca ctactcccat gccaagcgcc atggcacggg actgtcggcg gtcagcttcc
      121 gcatcgacaa ctacagcgac gtcgaaggca aggtcggcaa ggcgccggcg gagcagctcc
      181 tcgcgcgcat cgccaagctg gtgcaggaaa cgctgcgcgc cgaggactcg gtggggcgca
      241 tgggtcccgc actgttcagc gcaatcctgc cgagcaccgc ggccgagcag gcggtggtgt
      301 tcgcgcgccg cctgcgcgag cggctcgaca gcgcgcgcat cacgcatggc acggaggcga
      361 tccgcatccg caccagcttc ggcgtcgccg ccatggggca cgacaatccc ggctccatcg
      421 aggacctgct gaagatcgcc gccgagcgcc tggagaaggc ggcggcgaag agcgaggagc
      481 agcgcgccgc cgcgccgaac gacttcggcg ccaccctgca gcgcggcatc ggcaacgacg
      541 tcgagctcgc gatcaagatg ctcgacaagg cggccgtcga gcgcgcctcc gaggtgctgg
      601 agaagctcgg gccgctggtc aagaccacct gcgcccgcgt cggcatcgac ctgcaggagt
      661 tcctcttcct cctgcagtcc aagccctagc cgcggctact tcggcttgta gccgaacttc
      721 tggccgccga ggctcgcctc gtacatcagg ccgcccttgg cgacggtgaa caccgccatg
      781 cccttctggt agctgccggc cgtcgaggcg tggccgggcg cgccggcgct gctggcgccc
      841 gcgctggtcc cgccggtggt ggcctgcgcc gaggcgcccg ccgtgatcgc cacggcggtg
      901 gcctgcgcgc cgaactcgaa ctcgccggtg gtgaattcat cgaacgcgcg cttgtcctgg
      961 aagaagatga tctggctgta agcctggccg ccgagctgca ggcccacggt gagctgggtc
     1021 accgtcgcat cgccgacgtg cgcgcctttc gcgtagacgc ggcccaggcc gtgcgcgccg
     1081 ccgacgacca gcccgccctt gccgatcgtc ggaaacaccg catagccgta gctgtcgccg
     1141 aagaacttcg cgctctggcc cgcgccgcgg aatagcgcga tcgtctcggc gtactcgtcc
     1201 gacgtcggcg cagacgcgca tccggcgagg gccagcagca gaacccagtg cagcgcggcg
     1261 cgcatcctca gaagccgatc gtgatgccga tgttcgccac cgggaagtat ttgaagttct
     1321 tcagcttgtc ttcgagcctc tgctcctcgg ccgcggtctg ggcctgcgcc cgcgtgcact
     1381 ccggggtacc cgtcgccgac ccgcaattca ccgacaggct ggcgttgggc gagccctgga
     1441 acatcacccc gaggtcgaag tagaagttca ctcccttggt ccagacgttg ccgtagccga
     1501 ttccgaggta gggagcgacg ctgtggcccg gtttcaccgt gccgttgacc gcgacacgag
     1561 agtcgaggtt cgcctgcccg ttgacgtcca ccttgttgtt gttggcgatc acgcccgccg
     1621 agatacggaa cggcccgaac gggctccagt cgaggaacgc gttcaggttg ccgagcttca
     1681 gcttggcgtc gtagttcacg tcggcggttt tcacgtcggt gctatagctt ccggccgaca
     1741 ggccgacgcg tccgcccagc gtgggcgcga tgtcccagcc gaggtcaccg ccgaggccgg
     1801 tcgtgccgat gcgcagcccc gcccccgccg cctgcgccga gcccgcagcc gccgccagcg
     1861 ccgccacaag aaccgatctg ccgtagttca tcgccgactc cttagtgaat gaaagacatg
     1921 aatatcagat tttcgggtcc ggataaagcg ctgtaaatgt ttcaaggcaa agccaggcga
     1981 catggaatgc aatagccgct cgcacaatgc cgatcgcccc catcgactcg aatacttcca
     2041 gtgaggacag agccgcgatc agagcggggc agtgcgccag cgctccgacc acgttccatt
     2101 tcctcgcatt cgcttgaccc gagccaagta ccagcatcac cgcaataatc gccatcagca
     2161 gatacgcctg gatcagccca acccagacca tcgggttctg ctgcatgaaa ccaaacaggc
     2221 cgcttccagt cttccagcct gccgtcgtag cgacagcgct ccctaacgca accagcgcta
     2281 aaaccgttcc atggattcgc agcagccacc gccggaacgt ggctatgtga ctacctggca
     2341 aagaaggc
//
LOCUS       DASYJA010000055         7037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2005728, whole genome shotgun sequence.
ACCESSION   DASYJA010000055 DASYJA010000000
VERSION     DASYJA010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7037
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2005728"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1782
                     /gene="treZ"
                     /locus_tag="VGO02_01170"
     CDS             94..1782
                     /gene="treZ"
                     /locus_tag="VGO02_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEV7476327.1"
                     /translation="MAETQVVRQRSSSPFGAQITPEGVRFRLWAPAAKSVDVVFIETT
                     KQMHPAGDGWFEHLEPEAGAGTLYQYQIDGELLVPDPASRFQPKDADGPSEVIDPAFA
                     WEEWKGRPWEEAVIYELHVGTFTPAGTFLSVIEKIPHLKELGVTAIELMPLADFAGKR
                     NWGYDGVLPYAPDSAYGRPEDLKALVQAAHRAGLMVFLDVVYNHFGPKGNYLHRYAPQ
                     FFTERHQTPWGAAIDFSRREVRDFFIGNARYWLDEYRFDGLRFDAVHAIVDDSAKHIL
                     YEIRDAVPPEKHLVLENDANQARFLSKYAAQWNDDSHHAFHVLATGESQGYYASYIDG
                     PAKHLARCLAEGFAYQGEVSPFSGKPRGEPSRQLAPTSFVDFLQNHDQIGNRAFGERL
                     IALADEEQLRCLTAILLLAPSIPLLFMGEEWGCRQPFVFFCDFPGELGEAVRAGRRRE
                     FPQIKDMPDPRADATFQQCVLSWKEARSAAGKAWLAFYRELLQIRQERIVPLKARNGT
                     CRMLGRRAFEVRWDNLVLLANCGDEPVRVEAPKGKLVWSSGGPGDPWSVGWWLA"
     gene            1779..5870
                     /gene="treY"
                     /locus_tag="VGO02_01175"
     CDS             1779..5870
                     /gene="treY"
                     /locus_tag="VGO02_01175"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEV7476328.1"
                     /translation="MTDEVLGSYLDVFGNEKTIDPDTRQALERALGKPRSARRRAHPA
                     PGRCYEPEILARGGRLWGFGVQLYGVRSARNWGIGDFGDLRALLALSARHGASLVGVN
                     PLHAGDGASPYSPSSRHALNWRYLDLEAIPEYAASGKARALVASSAFQRRLKALREAE
                     LVDHEGVRRAKAQVLELLFEESSFNSASLPKGARTYALFEALREKFGAPWQSWPQPYR
                     DPGSKEISSFRQSHSKRIRFHEYVQFQARRQLDAVQAHAKELGMPIGLYVDLALGADA
                     GGAEVWADQQAYALDVSCGAPPDEFNPRGQDWGLPPFSPRALRASGCAPFAEMLRANM
                     PEGGALRLDHVMALMRLYWIPRGAKPERGGYVSYPFRDLIAVLARESRRRRCLVVGED
                     LGTVPRALRSALNEAGVLSYRPLLFEKGADGEYCPPAAYPREALVCAGTHDLPTWRGF
                     WAGTDIELRAALGLAVDPGREKERREADKAALARALAREGLDRTATAAHAFISRTPCK
                     IALVQPEDVFELLEQANLPGSVEQHPNWRRKQPIELEGWDADRRAATLYETMQERSPK
                     RALLAPAHAPVATYRLQLHRGFRFADAEALVPYLARLGVSHVYASPFLKARPGSMHGY
                     DVVDARSINPEIGTEAGLERLLRKLKAHGMGMVLDVVPNHMGVLHADNPWWQDVLEKG
                     RASRYAKFFDIDWTPGKVLLPVLGSHYGEALEKGEIRLVKEGGVWRVASDEINYRRFF
                     EIAELAAVRVEDPEVFEEINVLVKKIVRHPAVHGVRVDHPDGLADPARYLERLNALFE
                     RPPWVLVEKILADHEKFPEDWAAHGTTGYRFANLLTGLFVDASAEARFDRVYQRFTGE
                     RRTFEEIGHASRLLIMSTTLAAELDLLAGRLSGIADGNRGTRDYTRSGLRKALAEIAA
                     RFPVYRSYVGPRGASDTDRRYIGWAVSAARRSSRIADPGVFDFVESVLTLDAAPAAGP
                     RRAEMLAFAMRFQQFTAPVVAKGDEDTAFYRYHRLIALNEVGGNPARFGLSLKAFHAA
                     SEDRARRWPYTMLGTSTHDTKRSEDSRARLAVLSELPGQWRLALRRWSLLNRSRRSEI
                     GGALAPSRGDEYHFYQALIAVWPGGPSAELRERLKAYMLKAVREAKQHSSWINPDLEY
                     EAALERFVVESLQNELFLKDLKEVMPRLVHLGMLAGLSQALLKVASPGVPDYYQGSEV
                     WDLALVDPDNRRPVDFDSREKLLRGKGNLLRNLADGRAKLHVIQRGLEVRKNFPHLFH
                     GARYAPLHADGGMEEKVCAFLLRAHNDIVVAIAPRLFASLMSESDLAPAGEKIWRESR
                     LVLPQGDYTDVLTGARIAGGERPMKEILADFPVALLVSRLLLS"
     gene            complement(5867..6829)
                     /locus_tag="VGO02_01180"
     CDS             complement(5867..6829)
                     /locus_tag="VGO02_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7476329.1"
                     /translation="MKRLLFYVVLGMMSFGTAAQPLDSYPAKPLRFILPFPPGGGTDI
                     LGRLIAERLAAALGQPVVIENRGGAGGNVGTEAAARSAPDGYTIVLVAPSLAISPTLY
                     SKLNYDAVKDFAPVSLVATVPNVVITQPSLPGTLQEFAALARSRPGGLNFGSGGSGTS
                     NHLAGELFNIVAGVQLVHIPYKGVNLAMQDVLGGNVHFVVIGIPAAAPHIKAGKLRAL
                     AVLTPARSPALPDVPTAAESGMPAFEVTTWYGVLAPAGTPRPIVTRLNQELLKIMHSA
                     ELKAPLAASGTDPLTSTPEEFAAYIRQEIAKWGDVVRKANLKAD"
BASE COUNT         1115 a         2391 c         2455 g         1076 t
ORIGIN      
        1 cttcctcatc gtgctggccg gcgtggtatt cgagctggcg ctcgccttcg ggctgggcgg
       61 caaggactgg gcggcggaga tgctggagcg ctggtggccg aaacgcaagt agtcaggcag
      121 cgatcttcct cccccttcgg cgcacagatc acgcccgaag gcgtgcgctt tcgcctgtgg
      181 gcgcccgccg cgaaatccgt agatgttgtt ttcatcgaaa caacaaaaca aatgcatccg
      241 gccggcgacg ggtggttcga gcatctcgag ccggaggccg gagcaggcac gctgtaccaa
      301 taccagatcg acggcgagct gctggtgccc gatcccgctt cgcgcttcca gccgaaagac
      361 gccgatggcc cgagcgaggt gatcgatccg gcctttgcat gggaggagtg gaaaggccgg
      421 ccgtgggaag aagctgtcat ctacgagctg catgtaggta ctttcacgcc cgcaggaacc
      481 tttctttctg taatcgaaaa aattcctcac ctgaaagaac ttggcgtcac cgccatcgag
      541 ctgatgccgc tcgcggattt cgccgggaag cgtaactggg gctatgacgg cgtgctgccg
      601 tacgcacccg acagcgccta tggccggccc gaggacctga aggcgctggt gcaggcggcg
      661 caccgcgccg ggctgatggt gttcctcgac gtggtctaca accacttcgg cccgaagggc
      721 aactacctgc accgctacgc gccgcagttc ttcaccgaac ggcaccagac gccgtggggc
      781 gcggcgatcg acttcagccg gcgagaggtg cgcgacttct tcatcggcaa cgcgcgctac
      841 tggctggacg agtaccgctt cgacggcctg cgctttgacg ccgtgcatgc catcgtcgac
      901 gactcggcga agcacatcct ctacgagatc agggacgcgg taccgcctga aaagcacctg
      961 gtgctggaga acgacgccaa ccaggcgcgg ttcctgagca agtacgccgc gcagtggaac
     1021 gacgattccc accacgcctt ccacgtgctc gccaccgggg agtcgcaggg ctactacgcg
     1081 tcgtacatcg acggcccggc gaagcacctg gcgcgctgcc tcgccgaagg cttcgcctac
     1141 cagggcgagg tgtcgccctt cagcggcaag ccgcgcggcg agcccagccg ccagctggcc
     1201 cccacctcgt tcgtcgactt cctgcagaac cacgaccaga tcggcaaccg cgccttcggc
     1261 gaaaggctga tcgccctggc cgacgaagag caactgaggt gcctcacggc aatacttctg
     1321 ctggcgccct cgataccgct cctgttcatg ggcgaggagt ggggctgccg gcagccgttt
     1381 gtcttcttct gcgacttccc cggcgagctg ggcgaggcgg tgcgcgcggg gcggcggcgc
     1441 gagtttccgc agatcaagga catgcccgac ccgcgggcgg acgccacctt ccagcaatgc
     1501 gtgctgtcgt ggaaggaggc gaggagcgcg gccggcaagg cctggctggc gttctatcgc
     1561 gagctgctgc agatccgcca ggaaaggatc gtcccgctca aagcgcgcaa cggcacctgc
     1621 cggatgctcg gtcgcagggc gttcgaggtc cgctgggaca atctggtcct gctcgccaac
     1681 tgcggcgacg agccggtgag ggtcgaggcg ccaaaaggaa agctcgtgtg gtcgagcggc
     1741 gggccgggcg acccctggtc ggtcggctgg tggctcgcat gaccgacgaa gtccttggca
     1801 gctatctcga cgtcttcggc aacgagaaaa cgatcgatcc ggacacgcgg caggcgctcg
     1861 agcgggcctt gggtaagccg cgctccgcgc ggcggcgcgc acacccggcg cccgggcgct
     1921 gctacgagcc ggagatcctg gcgcgcggcg gccgcctctg gggtttcggc gtgcagctct
     1981 acggcgtgcg ctcggcgcgc aactggggca tcggcgactt cggcgacctg cgcgcgctgc
     2041 tcgcgctttc cgcgcggcat ggcgcgtcgc tggtcggcgt gaatccgctg cacgcgggcg
     2101 acggcgccag cccgtacagc ccgtcgagcc ggcatgcgct caactggcgc tacctcgacc
     2161 tcgaggcgat tcccgagtac gcggcatccg gcaaggcgcg agcgctggtt gcctcgagcg
     2221 ccttccagcg gcgcctcaag gccctgcgcg aggccgagct ggtcgaccat gaaggcgtgc
     2281 ggcgcgcgaa ggcgcaagtg ctcgagctgc tgttcgagga aagctccttc aatagcgctt
     2341 cactgccgaa aggcgcacgc acctatgcgc tgttcgaggc gctgcgcgag aagttcggcg
     2401 cgccctggca gtcgtggccg cagccgtacc gggatccggg ttcaaaggaa atttcttcat
     2461 tcaggcaaag ccactcgaaa agaatacgtt ttcacgagta cgttcaattc caggcgcgca
     2521 ggcagctcga cgccgtgcag gcgcacgcca aggagctcgg catgccgatc ggcctgtacg
     2581 tcgacctcgc gctgggcgcc gacgccggcg gcgccgaggt ctgggccgac cagcaggcct
     2641 acgcgctcga cgtttcctgc ggcgcgccgc ccgacgagtt caacccgcgc ggccaggact
     2701 gggggctgcc gcccttctcg ccgcgggcgc tgcgcgcgag cggctgcgcg ccgttcgccg
     2761 agatgctgcg cgccaacatg ccggaaggcg gcgcgctgcg cctggatcac gtgatggcgc
     2821 tgatgcgcct gtactggatc ccgcgcggcg cgaagccgga gcgcggcggc tacgtcagct
     2881 acccgtttcg cgacctgatc gccgtgctgg cgcgcgaaag ccgccgccgc cgctgcctgg
     2941 tggtgggcga ggacctcggc acggtgccgc gagccctgcg cagcgcgctg aacgaggcgg
     3001 gcgtgctctc ctaccggccg ctgctgtttg agaagggcgc cgacggggag tactgcccgc
     3061 cggccgccta tccgcgcgag gcgctggtct gcgccggcac gcacgacctg ccgacgtggc
     3121 gcggcttctg ggcgggaacc gacatcgagc tgcgcgccgc gctcggcctc gccgtcgacc
     3181 ccgggcgcga gaaggagcgg cgcgaggcgg acaaggcggc gctggcgcgc gccctggcgc
     3241 gcgagggcct ggaccgcacc gccaccgcgg cgcacgcttt catctcgcgc acgccgtgca
     3301 agatcgcgct ggtccagccc gaggacgtct tcgagctgct cgagcaggcg aacctgccgg
     3361 gcagcgtcga gcagcatccg aactggcggc gcaagcagcc gatcgagctc gagggctggg
     3421 acgccgatcg cagggccgcg acgctgtacg agacgatgca ggagcgcagc cccaagcgcg
     3481 cgctgctcgc gccggcgcat gcgccggtcg cgacctaccg gctgcaattg caccgcggct
     3541 tccgcttcgc cgatgccgag gcgctcgtgc cctacctcgc gcgcctgggt gtgagccatg
     3601 tctacgcctc gcccttcctc aaggcgcgcc cgggcagcat gcacggctac gacgtggtgg
     3661 acgcgcgcag catcaacccg gagatcggca ccgaggccgg gctggaacgg ctgctgagga
     3721 agctgaaggc ccacggcatg gggatggtgc tcgacgtggt gcccaatcac atgggcgtgc
     3781 tgcacgccga caacccctgg tggcaggacg tgctggagaa gggccgcgcc tcgcgctacg
     3841 ccaagttctt cgacatcgac tggacgcccg gcaaggtgct gctgccggtg ctcggcagcc
     3901 actatggcga ggcactagaa aagggggaga tcaggctggt gaaggaaggc ggcgtgtggc
     3961 gtgtcgcatc ggacgagatc aactaccggc gcttcttcga gatcgccgag ctggccgccg
     4021 tgcgcgtcga ggacccggag gttttcgaag aaatcaacgt attggtaaaa aaaatcgtgc
     4081 ggcatcccgc ggtccacggc gtgcgcgtcg accaccccga cggcctggcc gacccggcgc
     4141 gctatctcga gcggctgaac gcgctgttcg agcggccgcc ctgggtgctg gtggagaaga
     4201 tcctcgccga ccatgagaag tttcccgagg actgggccgc acacggcacc accggctacc
     4261 gcttcgccaa cctgctgacc ggcctgttcg tcgatgcgtc cgccgaggcg cgcttcgacc
     4321 gcgtctacca gcgcttcacc ggagagcgtc gcaccttcga ggagatcggg cacgcgagcc
     4381 gcctcctcat catgagcacg acgctcgccg ccgagctcga cctgctggcg ggacgcctgt
     4441 cggggatcgc cgacggcaat cgcggcaccc gcgactacac gcgaagcggc ctgcgcaagg
     4501 cgctcgccga gatcgccgcg cgcttcccgg tctaccgcag ctacgtcggg ccgcgcggcg
     4561 cgtcggacac cgaccgccgc tacatcggct gggcggtgag cgcggcgcgg cgctcgagcc
     4621 gcatcgccga tcccggcgtg ttcgacttcg tcgagagcgt gctgacgctg gatgccgcgc
     4681 ctgccgccgg gccgcggcgc gccgagatgc tcgctttcgc catgcgcttc cagcagttca
     4741 ccgcgccggt ggtggcgaag ggcgacgagg acaccgcgtt ctaccgctac caccgcctga
     4801 tcgcgctgaa cgaggtgggc ggcaacccgg cccgcttcgg cctgtcgctc aaggcgttcc
     4861 acgcggcgag cgaggaccgc gcgcggcgct ggccgtacac catgctcggc acttccaccc
     4921 acgacaccaa gcgctcggag gacagccgcg cccggctggc ggtgctctcg gagctgccgg
     4981 gccagtggcg gctcgccctg cgccgctgga gcctgctcaa ccgcagccgg cgctccgaga
     5041 tcgggggcgc gctggcgccc tcgcgcggcg acgagtatca cttctaccag gcgttgatcg
     5101 ccgtctggcc gggcggcccg agcgccgagc tgcgcgagcg cctgaaggcc tacatgctga
     5161 aagcggtgcg cgaggcgaag cagcactcga gctggatcaa cccggacctc gagtacgagg
     5221 cggcgctcga gcgcttcgtg gtcgagtcgc tgcagaacga gctgttcctg aaggacctga
     5281 aggaggtcat gccgcgcctc gtgcacctcg gcatgctggc cggcctgtcg caggcgctgc
     5341 tcaaggtcgc ctcgcccggc gtgcccgact actaccaggg tagcgaggtg tgggacttgg
     5401 ctctggtcga ccccgacaac cgccggccgg tcgatttcga ttcacgggaa aagcttcttc
     5461 ggggaaaagg caatctgctc agaaacctcg ccgacggccg ggccaagctg cacgtcatcc
     5521 agcgagggct cgaagtgcgg aagaacttcc cgcacctctt ccatggcgcg cgctacgcgc
     5581 cgctccacgc cgacggcggc atggaagaga aggtgtgcgc attccttctc agggcgcaca
     5641 acgacatcgt cgtcgcgatc gcgccccgcc ttttcgcctc actgatgagt gaaagcgacc
     5701 tcgctcctgc gggcgaaaag atctggcggg aatcccgtct cgtccttccg cagggcgact
     5761 acacggacgt gctgacgggc gcgaggatcg cgggaggcga gcgtcccatg aaggaaatac
     5821 tggcggactt tcccgtcgcg ctgctggtca gtcgcctgct tcttagctag tcagccttta
     5881 agttggcctt cctgaccacg tcgccccact tggcgatctc ctggcggata taggcggcga
     5941 attcctcggg cgtgctggtg agcgggtcgg tgccgcttgc cgcgagcggc gccttgagct
     6001 cggccgagtg catgattttc agcagctcct ggttgagccg cgtcacgatg ggtcgtggcg
     6061 tgccggccgg ggcgagcacg ccgtaccagg tcgtgacctc gaaggccggc atgccggact
     6121 cggcggcagt cggcacgtcg ggtagcgcgg gggaacgggc gggggtgagc acggcgagcg
     6181 cgcgcagctt ccccgccttg atgtgcggcg ccgccgcggg gatgccgatc accacgaaat
     6241 gcacgttgcc gccgagtacg tcctgcatcg cgaggttcac tcccttgtag gggatgtgca
     6301 ccagctgcac gccggcgacg atgttgaaca gctccccggc gaggtggttc gaggtgccgc
     6361 tgccgccgga gccgaagttc aggccgccgg gcctggagcg cgcgagcgcg gcgaactcct
     6421 gcagggtgcc ggggagcgac ggctgtgtga tcaccacatt cggcacggtc gccaccaagc
     6481 taactggcgc gaagtccttc accgcgtcgt agttgagctt cgagtagagc gtcgggctga
     6541 tggcgagcga gggcgcgacc agcacgatgg tgtagccgtc cggcgcggag cgcgccgcgg
     6601 cttcggtgcc gacattgccg ccggcgccgc cccggttctc gatcaccacc ggctggccca
     6661 gcgccgccgc cagccgctcg gcgatcaggc gtcccaggat gtcggtgccc ccgcccggcg
     6721 ggaacggcag gatgaagcgc agcggcttgg cggggtagga atcgagcggc tgcgcagccg
     6781 tcccaaagga catcattccc agaacaacat agaaaagcag ccgcttcatg ccgcgtattg
     6841 tgaactacca tcgccgtcgt gagcagcccg ccgtttcgcg ccgagcacat cggcagcttc
     6901 ctgcgacccg gggcgctgct gcgcggcgac caggccgccg accaggcgat caccgaggtc
     6961 atccgctggc aggagtcgct cggcctgcgc gtggtgaccg acggcgaatt ccggcgcgag
     7021 tcctggcggc tgggctt
//
LOCUS       DASYJA010000056         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_644531, whole genome shotgun sequence.
ACCESSION   DASYJA010000056 DASYJA010000000
VERSION     DASYJA010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644531"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VGO02_01185"
     CDS             complement(<1..246)
                     /locus_tag="VGO02_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV7476330.1"
                     /translation="MTKVKRIEGVLSPVVTPFRKDYSPDAERFVRHCKWLLKSGCAGL
                     AVFGTNSEANSMSVEEKLELLEALVKGGVPAASLMPGT"
     gene            complement(260..1216)
                     /locus_tag="VGO02_01190"
     CDS             complement(260..1216)
                     /locus_tag="VGO02_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7476331.1"
                     /translation="MSRLFALAALLLSLPAAAQPFPSKALRIVVPFTPAGSVDIATRA
                     TAHEMSKVLGQPVTVENKPGAGGNLGALDVARSAPDGYSMVMSTSGIQAINPALYSKM
                     ALDPNKELTSVAPIVSLSNVLVVHPSVPARSVKEVIALAKKDPGKWTYASSGNGTSIH
                     MSGAMFTQLTGTDILHIPYKGSAPAITDLLAGQTNMMFDNIPSALPHIRSGKLVALAT
                     TGAKRDPALPEQPTVAEAGVPGYESGVWFGLMVPAATPKEVIARLNSAAVQATKSPEF
                     IKRMTDLGYNIIPGSPEDMAAMLKAELARWAPIVKASGAKVD"
     gene            1252..2412
                     /locus_tag="VGO02_01195"
     CDS             1252..2412
                     /locus_tag="VGO02_01195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEV7476332.1"
                     /translation="MHLGDAVNIEDLRLMAKRRLPRAIFDFFDGGAEDETTLRDNRAA
                     FERIRLLPKVLVDVSKIDTTVSLFGSEAKMPLAIAPTGGISAGRPEAELILARAAKAW
                     GVPFTMATPSAFSIERVAQEVGGRLWFQLYAVRDKEFRANLVERAKRAGFEAILVTVD
                     LPVSGKRERDPRNGFVTPYKPSWRNSRDVLFKPAWLLGVMRHGLPGMANLEGYRFTTP
                     AGTDIVTAVGREMDAGLDWEYIKRLREQWPGKLLLKGVERPDDAERAVSVGCDGIVVS
                     NHGGRQLDGAAATLDALPAIASAVGNKITVLLDGGVRRGVDILKARALGAQAVLTGRA
                     TLFGAMAGAEPGARRALEILQTELVRAMQLCGVRSAAEIRPDLLASSERLSS"
BASE COUNT          396 a          833 c          905 g          432 t
ORIGIN      
        1 ggtgccgggc atcagggatg cggctggcac cccgcctttc accagcgctt ccagcagctc
       61 gagcttttcc tcgaccgaca tcgaattggc ttcgctgttg gtgccgaaca ccgcgaggcc
      121 ggcgcagccg ctcttcagca accacttgca gtgccgcacg aagcgctctg cgtcgggcga
      181 gtagtccttg cggaacggcg tgaccacggg cgagagcacg ccctcgatac gttttacctt
      241 tgtcatggcg ggcttcatat caatccacct tcgctccgga ggctttgacg atcggcgccc
      301 agcgcgcgag ctccgccttg agcatcgccg ccatgtcctc gggcgagccg gggatgatgt
      361 tgtagccgag gtcggtcatg cgcttgatga actcgggcga cttcgtcgcc tgcaccgccg
      421 ccgagttgag cctggcaatg acctccttcg gggttgcggc gggcaccatc aggccgaacc
      481 acacgcccga ctcgtagccg ggcacgccgg cctcggcgac ggtcggctgc tcgggcagcg
      541 ccgggtcgcg cttcgccccg gtggtggcga gggcgaccag cttgcccgac ctgatgtgcg
      601 gcagcgccga ggggatgttg tcgaacatca tgttggtctg cccggccagc aggtcggtga
      661 tcgccggtgc gctgcccttg tacgggatat gaagaatgtc ggtgccggtg agctgggtga
      721 acatggctcc cgacatatgg atgctggtgc cgttgccgct ggaggcgtag gtccacttgc
      781 cggggtcttt cttggccagc gcgatcacct ccttcacgct gcgcgcgggc accgacgggt
      841 gaaccaccag cacattgctg agcgagacaa tcggcgcgac cgaggtcagc tccttgttcg
      901 ggtcgagcgc catcttgctg tagagggcgg ggttgatcgc ctggatgccg ctggtgctca
      961 tcaccatcga gtagccgtcg ggcgccgagc gcgccacgtc cagggcgccg aggttgccgc
     1021 cggcgccggg cttgttctcg accgtcaccg gctggccgag gaccttgctc atctcgtggg
     1081 cggtggcgcg ggtagcgata tcgaccgatc cggcgggggt gaacggcacc acgatgcgca
     1141 gcgccttgga cgggaagggc tgggccgcgg cgggcaggga aaggagcagg gcggcgagag
     1201 cgaacaggcg cgacacgggc tggcctttcg aaggtagagt gacccgcaat tatgcacttg
     1261 ggggacgcgg tgaatatcga ggacctgcgc ctgatggcga aacgccgcct gccgcgcgcc
     1321 atcttcgact tcttcgacgg cggcgccgag gacgagacca ccctgcgcga caaccgcgcc
     1381 gccttcgagc ggatacggct gctgccgaag gttctggtgg atgtctcgaa aattgacacc
     1441 acggtaagtc tttttggcag tgaagcaaaa atgcctctcg ccatcgcccc caccggcggc
     1501 atcagcgccg ggcgccccga agcggagctg atcctggcgc gcgcggcgaa ggcatggggc
     1561 gtgcccttca ccatggcgac gccgtcggcg ttcagcatcg agcgggtagc gcaggaagtc
     1621 ggcggacggc tgtggttcca gctctacgcg gtgcgcgaca aggaattcag agcgaatctg
     1681 gtcgagcggg caaaaagggc gggcttcgaa gccatcctgg tgacggtgga cctgcccgtg
     1741 tccggcaagc gcgagcgcga tccgcgcaac ggcttcgtca ccccgtacaa gccgagctgg
     1801 cgcaactcgc gcgacgtgct gttcaagccg gcgtggctgc tcggcgtcat gcgccacgga
     1861 ctgccgggga tggcgaacct cgagggctac cgcttcacca ccccggccgg caccgacatc
     1921 gtgaccgctg tgggccgcga gatggacgcc ggcctcgact gggaatacat caagcgcctg
     1981 cgcgagcagt ggcccggcaa gctgctcctc aagggcgtcg agcggcccga cgatgccgag
     2041 cgcgcggtct cggtcggctg cgacgggatc gtcgtgtcga accatggcgg acggcagctc
     2101 gacggcgcgg cggccacgct ggatgctttg cccgctatcg cgtccgcggt aggaaacaag
     2161 atcaccgtcc tgctggacgg cggcgtgcgc cgcggcgtcg acatcctcaa ggcccgcgcc
     2221 ctgggcgcgc aggcggtgct taccgggcgc gcgacgctct ttggggcgat ggccggcgcc
     2281 gagccgggcg cgcggcgcgc gctcgagatc ctgcagaccg agctggtgcg cgcgatgcag
     2341 ctttgcgggg tgcgctcggc cgccgagatc cgccccgatt tgctcgcgtc gtcagagcgc
     2401 ctttcttcct gaaattacgc acgtagtccg gcgcgctgaa gacccactcc agcgcgtcgt
     2461 agttctgctc cgctgtacgg ccgccgtaga tgtcgttgcg caccaggcgc ttcaggccgt
     2521 cggggacgtg gaagatcatg cccaccagct cgttcgcgga gatctg
//
LOCUS       DASYJA010000057         2452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1371045, whole genome shotgun sequence.
ACCESSION   DASYJA010000057 DASYJA010000000
VERSION     DASYJA010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371045"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VGO02_01200"
     CDS             <1..490
                     /locus_tag="VGO02_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476333.1"
                     /translation="AKTGKPEMAKEEIDRLQALSKSAENSYWAEQIQVLVLAASAWRA
                     SAEGRKDDALKLMRGAADLEDSTEKHVAMENRLYPQREQLGDMLREYGQPAEALKAYK
                     VSMKNAPNRLRGYYGAAKAAQDSGNAKVAADYFNRLARLTRGADSDRAELAELKQVLA
                     RK"
     gene            487..936
                     /locus_tag="VGO02_01205"
     CDS             487..936
                     /locus_tag="VGO02_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV7476334.1"
                     /translation="MRRWVLALALALLPAAAPAEPGDEPDDQAKVDPDYAGGKKAVEA
                     KDWQTGIRLLSSAALRDTRNAEIQNLLGYAYRNSGELSLAFKHYNRALQLNPRHRGAH
                     EYLGEAYLMIGNLPKAEEHLAELRKICLIPCDEYAHLQSRIREYRHK"
     gene            933..1889
                     /locus_tag="VGO02_01210"
     CDS             933..1889
                     /locus_tag="VGO02_01210"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7476335.1"
                     /translation="MKVLLAVAVAFWSALAAAQGYPSKPIRFIAPFPPGGSSDVLCRL
                     LGQKLAEFTGQSVLVENRAGASGNIGHEFAAKQPADGYALVLTNNSALAINPHLFKRL
                     GFDPQDDFAPVSMVASAGQVLVVHPSVPATSVGELIALAKAKPGQLHFGSGGKGIPSH
                     IAGETFKVAAGVDIVHVPYKGTVQAVGDLVGGQVQLVFSDMVPAVPHIRAGKLRPLAV
                     TVGQRSAALPEVPTMDEAGLPGFRSVVWWAVLVPKGTPQDIVSRLNGHFGRIVAAPDV
                     QERYASLGVFTEHSTPQQVTERAKTDSREFARILKAAGVEAE"
     gene            1886..>2452
                     /locus_tag="VGO02_01215"
     CDS             1886..>2452
                     /locus_tag="VGO02_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7476336.1"
                     /translation="MIDLLLRGASVVDGTGAPAQVMDVGVKNGRIVSPDKATTTIDAG
                     GLCLMPGIVDVHTHYDAQVTWDPTLSPSVSLGVTTAVMGNCGFGIAPCPAPLRETMLR
                     NLSVVEGMDIDALFAGTRWDFETFPGYLDALERLRPWANVGVLAGHSTIRTAVMGEAA
                     SLNKNPTVDEMEAMKNHVRAAMDAGAAGF"
BASE COUNT          415 a          831 c          841 g          365 t
ORIGIN      
        1 cgcaaagacc gggaagcccg agatggctaa ggaggaaatc gaccggcttc aggccctgag
       61 caagtcggcg gagaacagct actgggcgga gcagatccag gtgctcgtcc tcgctgcgag
      121 cgcctggcgc gcaagcgcgg aagggcgcaa ggacgatgcg ctcaagctta tgcgcggggc
      181 agcggatctg gaggattcga ccgagaagca cgtggcgatg gaaaaccgcc tctatccgca
      241 gcgcgagcag ctcggcgaca tgctgcgcga gtacggtcag cctgccgagg cgctgaaggc
      301 ctacaaggtt tcgatgaaga atgcgcccaa ccgcctgcgc ggctactacg gcgccgccaa
      361 ggcggcgcag gactcgggca acgccaaggt ggcggcggac tacttcaacc gcctcgcgcg
      421 cctgacgcgc ggcgctgaca gcgaccgggc ggagctcgcc gagctcaagc aggtcctcgc
      481 ccggaagtga ggcgctgggt actcgcgctg gcgctcgccc tgcttcccgc ggcggcgccg
      541 gccgaacctg gcgacgagcc cgacgatcag gcgaaggtcg acccggacta cgccggcggc
      601 aagaaggccg tcgaggccaa ggactggcag accggcatcc gcctgctttc ctccgcggcg
      661 ctgcgcgaca cgcgcaacgc cgagatccag aacctgctcg gctacgccta ccgcaatagc
      721 ggcgagctga gcctcgcctt caagcactac aaccgcgcgc tgcagctcaa cccgcgccac
      781 cgcggtgcgc acgagtacct tggcgaggcc tacctcatga tcggcaacct gccgaaggcc
      841 gaggaacacc tcgccgagtt gcggaagatc tgcctcatcc cctgcgacga gtacgcgcac
      901 ctgcagagcc gcatccgcga gtaccggcac aagtgaaggt gctcctcgcc gttgcagtag
      961 ctttctggtc tgccctggca gcggcgcagg ggtacccgtc gaagccgatc cgcttcatcg
     1021 cgccgttccc gccgggcggg tcgagcgatg tgctctgccg gctgctcggg cagaagctcg
     1081 ccgagtttac cgggcaatcc gtgctggtcg agaaccgcgc cggcgcgagc ggcaacatcg
     1141 gccacgaatt cgcggcgaag cagccggccg acggctacgc gctggtgctg accaacaact
     1201 ccgcgctggc gatcaacccg catctgttca agcgcctggg cttcgacccg caggacgact
     1261 tcgcgccggt gtcgatggtg gcgagcgcgg gccaggtgct ggtcgtgcat ccgtcggtcc
     1321 cggcaaccag cgtcggcgag ctgatcgcgc tggcgaaagc aaagcccgga cagctgcatt
     1381 tcggctcggg cggcaagggt attccctcgc atatcgccgg tgagaccttc aaggtcgcgg
     1441 cgggcgtcga tatcgtgcac gtgccgtaca agggcaccgt gcaggcggtg ggcgacctgg
     1501 tgggcggcca ggtgcagctc gtcttctcgg acatggtccc cgccgtgccg cacatccgcg
     1561 ccggcaagct gcgtccgctc gccgtgaccg tcggccagcg ttcggcggcg ctgccggagg
     1621 tgccgaccat ggacgaggcc gggctgccgg gctttcgctc ggtggtgtgg tgggcggtgc
     1681 tggtgccgaa gggcacgccg caggacatcg tctcgcgcct caacgggcat tttggccgga
     1741 tcgtcgcggc gcccgatgtg caggagcgct atgcctcgct cggcgtattc accgagcaca
     1801 gcacgccaca gcaggttacc gagcgggcga aaaccgactc gcgtgagttc gctcgcatcc
     1861 tcaaggcggc gggcgtcgag gccgaatgat cgacctgctg ttgcgtggcg catcggtcgt
     1921 cgacggcact ggcgcgccgg cgcaggtcat ggatgtcggg gtcaagaacg gaagaatcgt
     1981 ttctccagac aaggcaacca ccacaatcga tgcaggcggc ctgtgtctca tgcccggcat
     2041 cgtcgacgtc cacacccact acgacgcgca agtcacctgg gacccgacgc tctcgccgtc
     2101 ggtgtcgctc ggcgttacca ccgccgtcat gggcaactgc ggcttcggca tcgccccgtg
     2161 cccggcgccg ctgcgcgaga cgatgctgcg caacctgtcg gtggtggagg gcatggacat
     2221 cgatgcgctc ttcgccggca cgcgctggga tttcgagacc tttccgggct acctcgacgc
     2281 gctcgagcgc ctgcggccct gggccaacgt cggcgtgctc gccggccact ccaccatccg
     2341 caccgcggtc atgggagagg ccgcctccct gaacaagaat ccgaccgtgg acgagatgga
     2401 ggccatgaaa aaccatgtcc gcgcggcgat ggatgccggc gcggccggct tt
//
LOCUS       DASYJA010000058         1525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2824156, whole genome shotgun sequence.
ACCESSION   DASYJA010000058 DASYJA010000000
VERSION     DASYJA010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2824156"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1127)
                     /gene="pyrC"
                     /locus_tag="VGO02_01220"
     CDS             complement(93..1127)
                     /gene="pyrC"
                     /locus_tag="VGO02_01220"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV7476337.1"
                     /translation="MDRLTLKRPDDWHLHLRDGASMASVLGDSARRFARAIVMPNLKP
                     AVRTTQQALHYRERILAALPDGAAFEPLMTLYLTDDTPPEEIARAKLSGRVFGVKLYP
                     AGATTHSAEGVTRISRCFHALEKMEQMGLPLLVHGESTDPAIDVFDREKAFLEDVLGP
                     LLERFPQLKVVLEHITTRDAAQFVEVTGPNVAATITAHHLLMNRNALFMGGMRPHHYC
                     LPVLKREEHREALVEAATSGNPKFFLGTDSAPHSRQAKETDCGCAGIYTAHAAVELYA
                     IAFEEAGALERLEGFAAEFGAQFYGLPQNQGTITLVREDWKVPETLPFGVERLVPLRA
                     GETLPWKLST"
     gene            complement(1139..>1525)
                     /locus_tag="VGO02_01225"
     CDS             complement(1139..>1525)
                     /locus_tag="VGO02_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV7476338.1"
                     /translation="LQALELPWPVILYEAPHRIAATLEDLRARFGEQREVLIARELTK
                     KFEEVARLALGQAAGWLAAQPHRQQGEFVLVLAPGGEAPPAIDADRVLAVLLEAVSPS
                     EAAKLAARITGAPRNELYRKALARAK"
BASE COUNT          225 a          504 c          564 g          232 t
ORIGIN      
        1 ggcctcttcg gcgagcgcgt tcaggccgtc cagcgacacc cgcccggcga gccgcgcatg
       61 ccatggccgc acggcagaga aaaccggatg ggctaggtcg agagcttcca ggggagggtc
      121 tcgcccgcgc gcagcggcac cagacgctcg acgccgaagg gcagggtctc cggcaccttc
      181 cagtcctcgc gcaccagggt gatcgtcccc tggttctgcg gcagcccgta gaactgcgcg
      241 ccgaactcgg cggcgaagcc ttccagccgt tcgagcgcgc cggcttcctc gaaggcgatc
      301 gcgtagagct cgacggcggc gtgcgcggtg tagatgccgg cgcagccgca atcggtctcc
      361 ttcgcctggc gcgaatgcgg cgcgctgtcg gtgccgagga aaaatttcgg gttgccggag
      421 gtcgccgcct cgaccagcgc ctcgcgatgc tcttcgcgct tgagcaccgg caggcaatag
      481 tggtgcggtc gcatgccgcc catgaacagc gcattgcggt tcatgagcag gtggtgcgcg
      541 gtgatggtag ccgcgacgtt gggcccggtg acttccacga actgggcggc gtcgcgcgtg
      601 gtgatgtgct ccagcacgac cttgagctgc gggaagcgct cgagcagcgg gccgaggacg
      661 tcctcgagga aggctttctc gcggtcgaaa acgtcgatcg ccgggtcggt cgattcgcca
      721 tgcaccagca gcggcaggcc catctgctcc atcttctcga gggcgtggaa gcagcgcgag
      781 atgcgcgtca cgccctcggc ggaatgggtg gtggcgcccg ccgggtagag cttgacgccg
      841 aacacccggc cggaaagctt ggcgcgcgcg atctcctccg gcggcgtgtc gtcggtcagg
      901 tacagcgtca tcagcggctc gaacgccgcg ccgtcgggca gggcggcgag gatgcgctcg
      961 cgatagtgca gcgcctgctg ggtcgtgcgc acggcgggct tgaggttcgg catcacgatg
     1021 gcgcgcgcga agcggcgtgc gctgtcgccc agcaccgagg ccatcgacgc gccgtcgcgc
     1081 aagtgcaggt gccagtcgtc gggacgcttg agagtgagcc ggtccatccg ctccgatttt
     1141 acttggcgcg cgcgagcgcc ttgcgataca gctcgttgcg gggggcgccg gtgatgcggg
     1201 cggcaagttt tgcagcctcg ctgggcgaga cggcttcgag caggaccgcg agcacgcgat
     1261 cggcgtcgat ggcgggaggc gcctcgccgc cgggagcaag caccagtacg aactcgccct
     1321 gctggcggtg cggctgggcg gcgagccagc cggccgcctg gccgagcgca agccgcgcca
     1381 cctcctcgaa tttcttggtg agctcgcgcg cgatcagcac ctcgcgctgc tcgccgaaac
     1441 gcgcgcgcag atcctccagc gtcgcggcga tgcggtgcgg cgcctcgtac agaatgacgg
     1501 gccagggcag ctcgagcgcc tgcag
//
LOCUS       DASYJA010000059         2900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_463372, whole genome shotgun sequence.
ACCESSION   DASYJA010000059 DASYJA010000000
VERSION     DASYJA010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463372"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VGO02_01230"
     CDS             complement(<1..419)
                     /locus_tag="VGO02_01230"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEV7476339.1"
                     /translation="MTREMQAKPAAHGQEISAREAWKLFGIMAEFFQATERLIGLPPA
                     VSVFGSARVKPGTPYYRLAEETARLLSDAGFAVISGGGPGLMEATNKGAFEGGGNAVG
                     LNIDLPREQPSSRYQNVSLRFKHFFARKVMFVRHATA"
     gene            418..>2900
                     /gene="polA"
                     /locus_tag="VGO02_01235"
     CDS             418..>2900
                     /gene="polA"
                     /locus_tag="VGO02_01235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003272571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEV7476340.1"
                     /translation="MSEENPEKTLLLVDGSSFLYRAFHALPELKSPKGEPTGAIYGVL
                     NMLRKLAADYKAQARACVFDARGKTFRDELYSDYKATRTAMPDDLSSQVQPLLEAVAA
                     IGWPVLVIDGVEADDVIATLAEQATRAGWRTVISTGDKDLTQLVDGHVTWVNTMSNEV
                     LDAEGVRKKFGVPPERIVDYLTLVGDAIDNIPGVDGVGPGFASKWLNQYGSLDNLIAN
                     ASQISGSRGENLRRKLDWLPKAKELVTVKRDVPLPVSFDQLIGKDDPERLRTIFQRFG
                     FKGNFFREETPGAAAAASTVAGPARRSQRTILEEHDLLDLVRRMQAAPLVAFDAACVG
                     EEPMSARLAGLSFAFGDEALYLPLAHEYPGARKQIDLSKAIDILQPWLERADYGKVSE
                     DAKFASHVLANHGVRLAGCVHDTLLESYVFEVHERHDLAALAQRHCGWALLTYDQVTG
                     KGAGRIPFSSVEIARATEYAAGKADCVLALHELLFQKIEKDEKLKFVYGRIEMPVLPV
                     LFRIERNGVLLDRGKLETQSHELGKEILQIEQQAYAAAGQPFNLGSPKQIQEILFERQ
                     KLPVKKKTPSGQPSTDEDSLAELALDHPLPRLILEHRTLSKLKSTYTDKLPRSINPAT
                     GRVHTTYSQTTAVTGRLSSNEPNLQNIPIRTAAGRRIRECFIASPGSKIVSADYSQIE
                     LRIMAHLSGDENLRRAFHEGEDVHRATAAEVFGVSIQNVEPDQRRVAKVINFGLIYGM
                     SSFGVAQNLGIDRGTAQTYVDRYFARFPGAKRYMDETRQRARELGYVETVYGRRLWLP
                     ELKSGAPVRRQAAERAAINAPMQGTAADL"
BASE COUNT          529 a          977 c          935 g          459 t
ORIGIN      
        1 aaggcggtgg cgtggcgcac gaacatcacc ttgcgtgcga agaaatgctt gaagcgcagg
       61 ctcacgttct ggtagcggct cgaaggctgc tcgcgcggca ggtcgatgtt gaggcccacc
      121 gcgttgccgc cgccctcgaa cgcgcccttg ttggtcgcct ccatcaggcc gggcccgccg
      181 cccgagatca ccgcgaagcc ggcgtcggag agcagccgcg ccgtttcctc cgcgagccgg
      241 tagtacggcg tacccggctt gacgcgcgcg ctgccgaaca ccgagaccgc cggcgggagg
      301 ccgatcaggc gctcggtcgc ctggaagaac tctgccataa tgccgaacag cttccacgcc
      361 tcgcgcgcgg agatctcttg gccgtgtgcc gccggcttcg cttgcatttc tcgggtcatg
      421 tcggaagaaa atcctgaaaa gacgctgttg ctggtcgacg gctcgtcctt cctgtaccgc
      481 gcattccacg cgctacccga gctgaagagc ccgaagggcg agccgaccgg cgccatctat
      541 ggcgtgctca acatgctacg gaagcttgcc gcggactaca aggcgcaggc gcgcgcctgc
      601 gtgttcgacg cccggggcaa gaccttccgc gacgagctct attccgacta caaggcgacc
      661 cggactgcga tgcccgacga cttgtcgtcg caggtgcagc cgctgctcga ggcggtggcg
      721 gcgatcggct ggccggtgct ggtgatcgac ggcgtcgagg ccgacgacgt catcgccacc
      781 ctcgccgagc aggcaacgcg cgccggctgg cgcacggtca tctccaccgg cgacaaggac
      841 cttacgcagc tcgtcgacgg gcacgtcacc tgggtaaaca cgatgtcgaa cgaggtgctc
      901 gacgccgaag gcgtaaggaa gaaattcggc gtgccgccgg agcgcatcgt cgactatctg
      961 acgctggtcg gcgatgccat cgacaacatt cccggcgtcg acggggtcgg accgggtttc
     1021 gcctcgaaat ggctgaacca gtatggttcc ctcgacaacc tgatcgcgaa tgcgagccag
     1081 atctcgggca gccgcgggga aaacctgcgc aggaagctcg actggctgcc gaaggcgaag
     1141 gaactggtga cggtgaagcg cgacgtgccg cttcccgtct ccttcgacca gctgatcggc
     1201 aaggatgatc ccgagcggct gcgcacgatc ttccagcgct tcggcttcaa ggggaacttc
     1261 ttcagagaag aaacccccgg cgctgccgcc gcagcatcca ctgtcgccgg cccggcgaga
     1321 cgcagccagc gcacgatcct ggaggaacac gacctgctcg atctcgtgcg ccggatgcaa
     1381 gcggcgccgc tggtcgcctt cgacgcggcc tgcgtcggcg aggagccgat gagcgcgcgc
     1441 ctggccggcc tgtcgttcgc cttcggcgac gaggcgctgt acctgcccct ggcgcacgaa
     1501 tatccaggcg ctcgaaagca gatcgacctg tcgaaagcga tcgacatcct gcagccctgg
     1561 ctcgagcgcg ccgattacgg gaaggtcagc gaagacgcga agtttgcttc gcacgtgctc
     1621 gccaatcatg gcgtgcgcct cgcgggctgc gtgcacgaca cccttctcga atcctacgtg
     1681 ttcgaggtgc acgagcgcca cgacctggct gcgctggcgc agcgccattg cggctgggcg
     1741 ctgcttacct acgaccaggt caccggcaag ggcgccgggc gcatcccgtt ctcgtcggtg
     1801 gagatcgcgc gggccaccga gtacgccgcc ggcaaggccg attgcgtcct cgccttgcac
     1861 gaattgcttt tccagaagat cgagaaagac gagaagctca agttcgtcta cggccggatc
     1921 gagatgccgg tgctgcccgt gcttttccgc atcgagcgca acggggtgct gctcgacagg
     1981 ggcaagctcg agacgcaaag ccacgagctg ggaaaagaaa ttctgcaaat cgagcagcaa
     2041 gcttatgccg ccgccggcca gccattcaac ctcggctcgc ccaagcagat ccaggaaatc
     2101 ctgttcgagc ggcagaagct gccggtgaag aagaagacgc cctcgggcca gccctcgacc
     2161 gacgaggatt cgcttgccga gctcgccctc gaccacccgc tgccgcgcct gatcctcgag
     2221 caccgcacgc tctccaagct caagtcgacc tacaccgaca agctgccgcg cagcatcaac
     2281 ccggcgaccg ggcgcgtgca cacgacgtat tcccagacca ccgcggtcac cggcaggctg
     2341 tcctcgaacg agcccaacct gcagaacatt ccgatccgca ccgcggcggg ccgccgcatc
     2401 cgcgaatgct tcatcgcctc gccggggtcg aagatcgtct cggccgacta ttcgcagatc
     2461 gagctcagga tcatggccca cctgtcgggc gacgagaacc tgcggcgcgc tttccacgag
     2521 ggcgaagacg tgcatcgcgc gactgccgcc gaggtttttg gggtttcaat tcagaatgta
     2581 gaacccgatc agcgccgcgt ggccaaggtg atcaacttcg gcttgatcta cggcatgtcg
     2641 tcgttcggcg tggcgcagaa cctgggcatc gaccgcggca cggcgcaaac ctacgtcgac
     2701 cgctacttcg cgcgcttccc cggcgcgaag cgctacatgg acgagacgcg ccagcgagcg
     2761 cgcgagctcg gctatgtcga gaccgtgtac ggccggcggc tgtggctgcc ggagctgaag
     2821 agtggcgcac ccgttcggcg gcaagccgcc gaacgggccg ctatcaatgc gccgatgcag
     2881 gggaccgcgg cggatctcat
//
LOCUS       DASYJA010000060         2335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1734046, whole genome shotgun sequence.
ACCESSION   DASYJA010000060 DASYJA010000000
VERSION     DASYJA010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1734046"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VGO02_01240"
     CDS             <1..928
                     /locus_tag="VGO02_01240"
                     /inference="COORDINATES: protein motif:HMM:NF037928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HEV7476341.1"
                     /translation="TDFSKDDTRMASFEDCANQRPLTDKGRDEARAVGGHVRRLGIPV
                     DRVYASPFCRTVETAMLAFGKAEKTQEVRGGPVRTDDPKRYDALKKLLSAPPASGQNN
                     VVSSHGNPFFALFGPPYLAEGEMAIVRPRARPASRSLAASGSRTGRRSSNRTLAEPAG
                     RNKMKTIWVVAADASRARVLQVASRKKLAEVTHLDNPEGRMHDRDLISDAHPRMRGTD
                     GPASDRQETSAVEHATEMFAKRIGQYLEKARTDHRYDELVVIAPPQLLGALRKEYGKE
                     VGRLVADEVAKDLSWLSKHELEGYFSKDGRAL"
     gene            complement(936..1466)
                     /locus_tag="VGO02_01245"
     CDS             complement(936..1466)
                     /locus_tag="VGO02_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEV7476342.1"
                     /translation="MVVAGAAEGEKKAAACAACHGEAGNSSNPQWPSLAQQPAQFIST
                     SLFMFREGNRKSDLMTPMAKPLSNADMNDLAAYFAAQKAAPPKHKSKPESVAAGPGLA
                     KKFNCSQCHGPQLLGQQHIPRLAGQQYDYLLEQLKGFKAQTRADIDGNMTSAAQALTP
                     QDIELLVDYLAGLGAQ"
     gene            complement(1540..>2335)
                     /locus_tag="VGO02_01250"
     CDS             complement(1540..>2335)
                     /locus_tag="VGO02_01250"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEV7476343.1"
                     /translation="KITRYDGIEANLDSPQGKVMVDNLDPSRAPGHFWKYLAMGPDGK
                     LYFNIGAPGNIVLPNYTQATIMRVDPDKGILETVAVGVRNSVGMDFNPKTKELWFTNH
                     ARDWLGDDSPNDTLHRVAGKSVPHFGYPFCHQGDTLDPEYGKNRSCAEFAKPSALLGT
                     HIAPLGMKFYNGKMFPAEYQNNIFIAMHGSWNRTVKQGYNVMRVKVDENGKAGKPTPF
                     LTGFMTDEKADPPMWGRPVDLLVMKDGSMLVSDDYNGIIYRVSYGK"
BASE COUNT          388 a          778 c          777 g          392 t
ORIGIN      
        1 gacggacttc agcaaggacg acacgcgcat ggcctccttc gaggactgcg ccaaccagcg
       61 cccgctcacc gacaagggcc gcgacgaggc gcgcgccgtc ggcggccatg tcaggcgcct
      121 cggcatcccg gtcgaccgcg tctatgcgag cccgttctgc cgcacggtcg agaccgcgat
      181 gctcgctttc ggcaaggccg agaaaacgca ggaagtgcgc ggcggtccgg tgcggaccga
      241 cgatccgaag cgctacgatg cgctgaagaa gctgctgtcg gcgccgcccg cgtcggggca
      301 gaacaacgtc gtctcgagcc acggcaatcc gttcttcgcc ctgttcggcc cgccctacct
      361 cgccgagggt gaaatggcga tcgtgcgccc gagggcaaga ccggcttcac gctcgctggc
      421 cgcatcaggc tcgaggactg gccgacgctc aagtaaccga accctcgcag aacccgcggg
      481 tcgaaacaag atgaaaacga tatgggtggt ggcggccgac gcgagccgcg cgcgcgtact
      541 gcaagttgca agccgcaaga agctggccga ggtcacgcac ctcgacaacc ccgagggccg
      601 catgcacgac cgcgatctca tcagcgacgc ccatccgcgg atgcgcggaa ccgacggccc
      661 cgccagcgac cgccaggaaa cctccgccgt cgagcacgca acggaaatgt tcgcgaagcg
      721 catcggtcag tacctggaga aggcccgcac cgaccatcgc tacgacgagc tggtggtcat
      781 cgcgccgccg cagctcctcg gcgcgctgcg caaggagtac ggaaaggaag tcggcaggct
      841 cgtcgccgac gaggtggcga aggacctttc ctggctcagc aagcacgagc tggaaggcta
      901 cttctcgaag gacggccggg cgctctgacc cgcctctact gggcgccgag ccccgctaga
      961 tagtcgacga ggagctcgat gtcctgcggg gtgagcgcct gcgccgccga ggtcatgttg
     1021 ccgtcgatgt cggcccgcgt ctgcgccttg aagcccttga gctgctccag cagatagtcg
     1081 tactgctgcc ccgcgagccg cgggatgtgc tgctggccga ggagctgcgg gccgtggcac
     1141 tggctgcagt tgaacttctt ggcgaggccc ggcccggcgg ccacgctctc cggcttgctc
     1201 ttgtgcttgg gcggcgccgc cttctgcgcc gcgaagtacg ccgccaggtc gttcatgtcc
     1261 gcgttcgaca gcggcttggc catcggcgtc atcaggtcgc tcttgcggtt gccctcgcgg
     1321 aacatgaaca gcgaggtcga gatgaactgc gccggctgct gggcaagcga aggccactgc
     1381 ggattgctcg aattgcccgc ctcgccgtgg caggcggcgc aggccgccgc cttcttctcg
     1441 ccctcggcgg cgcccgcgac gaccattgcc ggcaggccca gaaataaaaa cgcgacggtg
     1501 caagccgtcg cgtttccagc acccaacttc aaaaaaaact cacttgccgt aggagacgcg
     1561 gtagatgatg ccgttgtagt cgtccgacac cagcatcgag ccgtccttca tcaccagcag
     1621 gtcgaccggc cggccccaca tcggcggatc ggccttctcg tcggtcatga atccggtgag
     1681 gaacggcgtc ggcttgccgg ccttgccgtt ctcgtcgacc ttgacgcgca tgacgttgta
     1741 gccctgcttg acggtgcggt tccacgagcc gtgcatggcg atgaagatgt tgttctggta
     1801 ctcggccggg aacatcttgc cgttgtagaa cttcatcccc agcggcgcga tgtgcgtgcc
     1861 gagcagcgcc gagggcttgg cgaactcggc gcacgagcgg ttcttgccgt actccgggtc
     1921 gagcgtgtcg ccctggtggc agaacggata gccgaagtgc ggcaccgact tgcccgccac
     1981 gcggtgcagc gtgtcgttcg gcgagtcgtc gcccagccag tcgcgcgcgt ggttggtgaa
     2041 ccacagctcc ttggtcttcg ggttgaagtc catgccgacg gagttgcgca cgcccaccgc
     2101 gacggtctcg aggatgccct tgtccgggtc gacccgcatg atcgtagcct gcgtgtagtt
     2161 cggcagcacg atgttgcccg gcgcgccgat gttgaagtac agcttgccgt cggggcccat
     2221 cgccaggtac ttccagaaat gccccggcgc gcggctgggg tcgaggttgt cgaccatcac
     2281 cttgccctgc gggctgtcga ggttggcctc gatgccgtcg tagcgggtga tcttg
//
LOCUS       DASYJA010000061         3424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1371609, whole genome shotgun sequence.
ACCESSION   DASYJA010000061 DASYJA010000000
VERSION     DASYJA010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371609"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VGO02_01255"
     CDS             <1..317
                     /locus_tag="VGO02_01255"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476344.1"
                     /translation="GSTGSAGAGASGSAAKSDGGAEAMFKALDKNKDGFISKEEAKGT
                     PHDKDFTTLDKNGDGKLSREEHAAAPEHSGSSASGSSSAPSAPSSSGASSGSSESKGS
                     KY"
     gene            complement(381..653)
                     /locus_tag="VGO02_01260"
     CDS             complement(381..653)
                     /locus_tag="VGO02_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476345.1"
                     /translation="MARPPKVMVLQIAAEMLGGRRKLRTFLRASSADLASWLAGTKQP
                     PERAFLKALELILDDLDAGGRRLSKISRARRESAVVLKARREKSPR"
     gene            885..1175
                     /locus_tag="VGO02_01265"
     CDS             885..1175
                     /locus_tag="VGO02_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476346.1"
                     /translation="MTRERRTIPLMREHSAAARDCPLAPPAHASVYSRTLHRACLILG
                     GVGQLAVHLEVTEIELRHWMGGVGGAPQPPEAVFLRAVEVLLLHVASKGSAN"
     gene            1242..1508
                     /locus_tag="VGO02_01270"
     CDS             1242..1508
                     /locus_tag="VGO02_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476347.1"
                     /translation="MAASVYSRTLQKAAELIGGRARLCRHLHVPAAELQKWIGDKAVP
                     PMGVFLRAVDLIIDESPLPGNSSDPADPDAPRDCSSGDGEPTRY"
     gene            1781..2689
                     /locus_tag="VGO02_01275"
     CDS             1781..2689
                     /locus_tag="VGO02_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476348.1"
                     /translation="MAILVAGGARAQDPPQATAGFEVGVRYWLSSGMTKRAHDASSFS
                     PILANPTSVLEYDDMWANSVELHARKAFGEKWWVKGYLGLGRINTGTFTDQDFIILNG
                     QKFFSETVSGMNGRLSYGTIDLGHEVWRRENTAFSLFAGYSQWNEHVTSYGLSSTTGG
                     GSLGDSQPAVGNDLTWKAARLGGAMRAERGRTRYTAEVVLVPYASYRNEDSHFLRSAA
                     VGSSSGALGPVPNVIGEGRGTGAQFEIELRRSYPQYLGLEFGLGLRYWTLSSTHGTQE
                     QAGLNFPLVSLKSERLGGLFSITKAW"
     gene            complement(3024..>3424)
                     /locus_tag="VGO02_01280"
     CDS             complement(3024..>3424)
                     /locus_tag="VGO02_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006397467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEV7476349.1"
                     /translation="SAQSQQSQSSQQSEKGSAGSGATRDAPIAGAVPLGITVEATAAV
                     VKGWRASKLVGASVYNDQNQRIGKIDDMIVSPDGKVSVAVIDVGGFLGLGKHRVAISV
                     DQFSDINAKKLVLPNATKEALKALPEFTYA"
BASE COUNT          577 a         1118 c         1143 g          586 t
ORIGIN      
        1 cgggcagcac cggctcggcc ggcgccggcg cgtcgggttc cgcagccaag tccgatggtg
       61 gcgcggaggc gatgttcaaa gccctggaca agaacaagga cggcttcatc agcaaagagg
      121 aagcgaaggg cacgccgcac gacaaggatt tcaccaccct cgacaagaac ggagacggca
      181 agctgtcaag ggaagagcac gccgccgcac ccgagcattc cggcagcagc gcgtcgggca
      241 gcagttccgc gccgagcgcg ccgagcagca gcggggcctc gagcggcagt tccgagagca
      301 agggctccaa gtactgagcg ccgtttgccc gagacgaaag ccccgctggt gcggggcttt
      361 tttgttttcc gtcgctctgg ctaccgcggc gatttttccc gccgcgcctt gagcaccacg
      421 gcgctttccc ggcgcgcgcg agagatcttc gacaggcgcc gcccgccggc gtcgagatcg
      481 tcgaggatca gctccagcgc cttcaggaag gctcgctccg gcggctgctt ggtgccggcg
      541 agccagctcg ccaggtcggc ggaggaggcg cgcaggaagg tgcgcagctt gcgccgtccg
      601 ccgagcattt ccgcagcaat ctgcagcacc atcactttcg gcggccgcgc cacgctccta
      661 ggattagccg ggcgtccgac cgcgcacaag tagcggagcc gtgatttcct tgtaaagggt
      721 tcaccgtcgg gacgcacccg acgcatcgga ccccggcttg caaaggacgt acccgcgcgg
      781 tatatgtcgt tgtgtctcct ctgcttcgca gccgcgctcg cttccattcg acgcccgggc
      841 gcgctgaaga acgacaacac gttttgagag atggcgactt gtttatgacc cgcgaacgtc
      901 gcacgatacc gctcatgcga gaacactccg ccgccgcgcg ggactgcccg ctggcgccgc
      961 ccgcgcacgc cagcgtgtat tcgcgcacgc tccaccgggc ctgcctgatc ctgggcgggg
     1021 ttgggcagct ggccgtgcac ctcgaagtga cggagatcga gctgcggcac tggatgggcg
     1081 gtgtgggcgg tgccccgcag ccccccgagg cggtgtttct ccgcgcggtc gaggtcctgc
     1141 tgctgcatgt ggcgagcaag ggttcggcga actgacgctg cgattgtgcg gtttccccga
     1201 ttgggacatg atttcggggc gcaacaaccg aaagaacgat agtggcagct agcgtctaca
     1261 gcagaaccct gcagaaagcg gccgagctga tcggtgggcg ggccaggctt tgccgccacc
     1321 tgcacgtgcc ggcggccgag ctgcagaaat ggatcggcga caaggccgtg ccgccgatgg
     1381 gcgtcttcct gcgggcggtc gatctcatca tcgacgaaag cccgctgccc ggaaactcgt
     1441 ccgatccggc tgaccccgac gcgccgcgcg actgctcatc gggcgacggc gagccgacgc
     1501 ggtactaaat ccgcccgatc caaaaaaacg ccccggtggc tcgcgccagc ggggcgttct
     1561 gcttctgtac tgctaaaggc agccggccta gttggcctga ctcctcaagt tgcgggtaga
     1621 tccgccagag tcctgccggc cggtgttgcc ttggtcatct ctcacaggat cacctccttt
     1681 cgtggttgcc agactaaagt gtagcaaacc gcgaatcggc aatcggttgt agagtagggc
     1741 gcgacaaggg ggatccatgc ggagggcttg gtttcttgtt gtggcgatcc tcgttgccgg
     1801 cggcgcgcgg gcgcaggatc cgccgcaggc aacggccggg ttcgaagtcg gcgtgcgcta
     1861 ctggctgagc agcggaatga ccaagcgtgc gcacgacgca tcctcgttct cgccgattct
     1921 cgccaatccg acctcggtgc tggagtacga cgacatgtgg gcgaactccg tcgagctgca
     1981 tgcgcgcaag gcgttcggcg agaagtggtg ggtcaagggc tacctcggcc tgggccgcat
     2041 caacaccggg actttcaccg accaggactt catcatcctt aacgggcaga agttcttttc
     2101 cgagacggtc tccggaatga acggcaggct gagctatgga acgatcgacc tcggccacga
     2161 agtctggcgg cgggagaaca cggcgttcag cctgttcgcc ggctacagcc agtggaacga
     2221 gcatgtcacg tcctatggcc tgtcgagcac gaccggcggc ggctcgctcg gcgactccca
     2281 gccggcggtc ggcaacgatc tcacctggaa ggccgcgcgg ctcggcggcg cgatgcgcgc
     2341 ggagcgcggc cgcacccgct atacggccga ggtagtgctg gtgccctatg cgagctaccg
     2401 caacgaggac agccacttcc tgcgctcggc ggcggtaggg agcagctcgg gcgcccttgg
     2461 cccggtgccc aacgtgattg gcgaagggcg cggcaccggt gcgcagttcg agatcgagtt
     2521 gcggcgcagc tacccgcagt acctcggtct ggagttcggc ttgggcctgc gctactggac
     2581 gctttcctcg acccacggca cccaagagca ggccggcctc aatttcccgc tggtgtcgct
     2641 gaagtcggag cgcttaggcg gcctgttcag cattaccaag gcctggtagg cggcctactc
     2701 tcgcacgcgg gagatcttat tcacgtcggg caggcggcgc agctcatgca ccccgcgcga
     2761 tccagcgcag aaaacgcgaa gcgccgattc atgtacgtct tgctacctga tcgcagcatc
     2821 ggcctttgca cagagtccgc agcagaggtc cgacgggctc gagatcggca tcggcgtacg
     2881 ctactggaag ctgactgcgt ccaacggtca cgaaaccgcg ggcggcgtga gcttgccgat
     2941 cgtcaacctg gaatcggaac gccagggctt caccttcacc gtggcgaaga cctggtaggc
     3001 actacccccg cacgcggcgg ccgtcaggcg taggtgaatt cgggcagcgc cttgagcgct
     3061 tccttcgtcg cgttcggcag caccagcttc ttcgcgttga tgtcgctgaa ctggtcgacc
     3121 gagatcgcca cgcggtgctt gcccaagccg aggaagccgc cgacgtcgat caccgccacg
     3181 gacaccttgc cgtcggggct cacgatcatg tcgtcgatct tgccgatgcg ctggttctgg
     3241 tcgttgtaca cgctcgcacc gaccaatttg gatgcgcgcc agcccttgac gacggcggcg
     3301 gtcgcctcga cggtgatccc gaggggcacc gcgccggcga tcggcgcatc gcgcgtcgcc
     3361 ccgctgcctg cgctgccttt ctcggactgc tggctgctct gggactgctg actttgcgcg
     3421 ctgg
//
LOCUS       DASYJA010000062         6526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_645688, whole genome shotgun sequence.
ACCESSION   DASYJA010000062 DASYJA010000000
VERSION     DASYJA010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6526
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645688"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="VGO02_01285"
     CDS             <1..146
                     /locus_tag="VGO02_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013428648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEV7476350.1"
                     /translation="IAIDSYREIIAYLGDADPTSRRLMEEILAKEEEHADDLSSLLED
                     IAR"
     gene            153..971
                     /locus_tag="VGO02_01290"
     CDS             153..971
                     /locus_tag="VGO02_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEV7476351.1"
                     /translation="MPRGLWKGAISFGLVNVPVELIPGEKRSGELHFTMLDKRDMAPV
                     GYQRVNKSSGKTVPWKEIVKGYEYEDERYVVLSDEDFRRANVQASRTVEILAFVERAD
                     IQPQYFSTPYYLLPGKRGEKAYALLRDTLAKTGKVGIASFVIRSKQHLAALLPEGDAL
                     LLITLRYVDELREPKELDVPAAKVTARERSLAIKLVEDMSDKWQPGKFHDTYRKDILA
                     RVKQKVKAGQTEEITAPDKDEAKPKAEVIDLMAALKKSVSARSTRAASKKRRAA"
     gene            971..2062
                     /locus_tag="VGO02_01295"
     CDS             971..2062
                     /locus_tag="VGO02_01295"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV7476352.1"
                     /translation="MPRRQIRYAVVGLGHIAQVAMLPAFANARRNSRLAALVSSDPSK
                     RRLLAEKYGVEQTYSYEQYDECLRSGIEAVYIALPNNLHCEYAVRAARAGVHVLCEKP
                     LALTEEECERMAAAARESGVKLMTAYRLHFERANLEAGEIARSGRIGEPKLFTSTFCT
                     PVAPGDIRARRETGGGAFWDIGIYCINAARSLFRGEPIEVLAGTAGKLGEVEETASAV
                     LRFPGERSAAFTCSFGTQKVSEYRLAGDKGDLAVEPAYEYAVALRHRLTIDGETRERR
                     FARRDQFAPELLYFSDCVLQNHEPEPGADEGLADVRVIRALYRSAETGRPVELAPFEK
                     RERPSLEQEIRRPPVEKPEVIKATPPAGH"
     gene            2076..2438
                     /locus_tag="VGO02_01300"
     CDS             2076..2438
                     /locus_tag="VGO02_01300"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEV7476353.1"
                     /translation="MKTKALAVLIPAIFVATAAIAQTKEPAASDSKPAVPSAQDRANN
                     RENVSDATITTKVKAALAADVGLKTVTGINVDTEKGGVVTLKGKVNSADIKKHAGEVA
                     KKVNGVKSVKNQLQVNKG"
     gene            2453..2836
                     /locus_tag="VGO02_01305"
     CDS             2453..2836
                     /locus_tag="VGO02_01305"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEV7476354.1"
                     /translation="MKAKALAVLIPALFLATAALAQSTTTPREPATREPSKEAAPNDA
                     KGSGSVSREDMSDATITAKVKSALAADVGLKTVTGINVDTDNGGVVTLKGKVDSADMK
                     QRAEQVAKKVNGVKSVKNELQVTKG"
     gene            2841..3083
                     /locus_tag="VGO02_01310"
     CDS             2841..3083
                     /locus_tag="VGO02_01310"
                     /inference="COORDINATES: protein motif:HMM:NF021471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEV7476355.1"
                     /translation="MAAGVPLLSVARSRDGVWSVNERGPDRVAIAYFPSQWGAMKHAV
                     RVARAKRRCRVAVHAADGGVRLSRDYSSAPGAVETP"
     gene            3080..3559
                     /locus_tag="VGO02_01315"
     CDS             3080..3559
                     /locus_tag="VGO02_01315"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEV7476356.1"
                     /translation="MSSGAFALIAGVVYTLLGIAGLFPVAGGYLGLFPMNVPLGALHL
                     AVGVCGLAAASVQRYGRLYARCVAAILGVLGAMGLVAGLDGAFGLMPLHGHNVWLHLG
                     TAAIAAYFGWRPQIPVLREELEAQDRRDGFGDRRETIATVSRERRHGAYDRRTPLGA"
     gene            3563..4870
                     /locus_tag="VGO02_01320"
     CDS             3563..4870
                     /locus_tag="VGO02_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEV7476357.1"
                     /translation="MRTLAAALSILLLAACGETSRIPEAAQTGPNPQLPPPQSSLVPT
                     VKIAPAKGWPAGATPKAAPGFAVTAFADTLDHPRWVYVLPNGDVLVAETNAPPKPEDG
                     KGVKGWAMKKFMARAGATAPSANRITLLRDTDGDGKAETQTVFLEGLKSPFGMALVGN
                     DLYVANTDAVMKFKYKEGQTRIDAPGEKVADLPGGPLNHHWTKNIIASPDGKRLYATV
                     GSNSNAAENGIDKEERRAAILEIDPASGATRLFASGLRNPNGMGWQPHDGRLWTVVNE
                     RDELGSDLVPDYLTSVKDGAFYGWPYSYYGRNVDKRVKPQRPDLVDKAIVPDYALGSH
                     VAPLGLVFYDKTLFPEHFAGGAFIGQHGSWNRKPLSGYRVVFVPFKDGRPSSPAPVDI
                     LTGFVDADGKAMGRPVGVAVDGKGALLVADDVGNMVWRVTPAP"
     gene            4923..5351
                     /locus_tag="VGO02_01325"
     CDS             4923..5351
                     /locus_tag="VGO02_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV7476358.1"
                     /translation="MLVNEAMSRDVRVANPQQRISEAAKTMAEIDVGVLPVGENDRLV
                     GMITDRDIAVRAVAAGKGPDTLVREVMTVDVKYCFEDEDLVHVSKNMAEQQVRRLPVV
                     NREKRLVGILALSDIAAKEGSRVTGRAIEGISRPGGQHTS"
     gene            5399..6508
                     /locus_tag="VGO02_01330"
     CDS             5399..6508
                     /locus_tag="VGO02_01330"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEV7476359.1"
                     /translation="MASERPPFRADHVGSLLRPPELKQARADLKAGKISRQSLTEIEN
                     QSIRSAVALQEAAGLQAVTDGEFRRAFWHVDFLTGFEGIAATQGQYALKFHGEGGAES
                     ETKSMMVVTGKVKRAKPIMVSHFEFLRKATSKTAKLCIPAPTYLHMRGGRKVVDAKAY
                     PDIEEFWSDIAGAYREEIADLARAGCKYLQIDDVSFATLCDESIRAQVRRDGEDPAKL
                     PAKYASVISSLLKGKPKDMSVTMHTCRGNHASMWMAEGAYDAVAEGIFQTDVDGFFLE
                     YDTERAGGFEPLRFVPKGKKVVLGLISSKNPRLESRDALKRRVEQAAKYVPLENLCLS
                     PQCGFASSEVGNKLTTDDQRRKLELAVETAREIWG"
BASE COUNT         1240 a         2178 c         2180 g          928 t
ORIGIN      
        1 gcatcgccat cgacagctac cgcgagatca tcgcctacct cggcgacgcc gacccgacct
       61 cgcgccgcct gatggaggag atcctggcga aggaggagga gcacgccgac gacctctcca
      121 gcctgctcga ggacatcgcg aggtagcccg gcatgccgcg cggactctgg aaaggcgcca
      181 tcagcttcgg gctggtgaac gtgccggtgg aactcatccc gggcgaaaaa cgctcgggtg
      241 agctccattt caccatgctc gacaagcgcg acatggcgcc ggtgggctac cagcgcgtca
      301 acaagtcgag cggcaagacc gtgccgtgga aggagatcgt caagggctac gagtacgagg
      361 acgagcgcta cgtggtgctg tccgacgagg acttccgccg cgccaacgtg caggccagca
      421 ggacggtcga gatcctcgcc ttcgtcgagc gcgccgacat ccagccgcag tatttctcga
      481 cgccctacta cctgctcccc ggcaagcgcg gcgagaaggc ctacgcgctt ctgcgcgaca
      541 cgctggcgaa gaccggcaag gtcggcatag cgagcttcgt catccgcagc aagcagcacc
      601 tcgccgccct gctgcccgag ggcgacgcgc tgctcctgat cacgctgcgc tacgtggacg
      661 agctgcgcga gccgaaggag ctggatgtgc cggcggcgaa ggtcaccgcc agggaacgca
      721 gcctcgccat caagctggtc gaggacatga gcgacaagtg gcagccgggg aaatttcacg
      781 acacctaccg caaggacatc ctggcgcggg tgaagcaaaa ggtgaaggct ggccagaccg
      841 aggagatcac cgcgccggac aaggatgagg cgaagcccaa ggccgaggtg atcgacctga
      901 tggccgcgct gaaaaagagc gtttcggcca ggtcgaccag agcagcgagc aaaaagcgca
      961 gagccgcctg atgccgcgca gacagatccg ctacgccgtc gtcggcctcg gccacatcgc
     1021 ccaggtcgcg atgctgcccg ccttcgccaa cgcgcggcgc aactcgcgcc tggccgctct
     1081 tgtttcttcg gatccttcga aaaggaggct tcttgccgag aaatacggag tcgagcagac
     1141 ctactcctac gagcagtacg acgagtgcct gaggagcggc atcgaagcgg tgtacatcgc
     1201 gctgcccaac aacctgcact gcgaatacgc ggtgcgcgct gcgcgcgccg gcgtgcacgt
     1261 gctgtgcgag aagcccctcg cgctcaccga ggaggaatgc gagcgcatgg cggccgcggc
     1321 gcgcgagtcc ggcgtcaagc tgatgaccgc ctaccgcctg cacttcgagc gcgccaacct
     1381 cgaggcgggc gagatcgcgc gctccggccg catcggcgag ccgaagcttt tcacctcgac
     1441 gttctgcacg ccggtcgccc ccggcgacat ccgcgcgcgg cgcgagaccg gcggcggcgc
     1501 cttctgggac atcggcatct actgcatcaa cgcggcgcgc agcctgttcc gcggcgagcc
     1561 gatcgaggtg ctggcgggca ccgccggcaa gctcggcgag gtggaggaga cggcgagcgc
     1621 ggtgctgcgc tttcccggcg agcgcagcgc cgccttcacc tgcagcttcg gcacgcagaa
     1681 ggtttccgaa taccgcctcg cgggcgacaa gggcgatctc gcggtcgagc ccgcctacga
     1741 gtacgccgtg gccttgcgcc accgcctgac catcgacggc gagacgcgcg agcggcgctt
     1801 cgcgcgccgc gaccagttcg cccccgagct gctgtacttc tcggactgcg tgctgcagaa
     1861 ccatgagccc gagcccggcg ccgacgaagg gctcgccgac gtgcgcgtga tccgcgcgct
     1921 ctaccgatcc gccgagaccg gccgtcccgt cgagctggcg ccgttcgaga aacgtgaacg
     1981 gcccagcctc gagcaggaga tccgccggcc tcccgtggaa aaacccgaag ttatcaaggc
     2041 aaccccaccc gctggacatt gacaggagac gttccatgaa aaccaaagca ctagccgttc
     2101 tgattcccgc aatttttgtg gccactgccg ccatcgcgca gaccaaggag ccggcggcct
     2161 ccgacagcaa gccggccgtg ccgagcgcgc aggaccgcgc caacaaccgc gaaaacgtgt
     2221 ccgacgcgac catcaccacc aaggtgaagg ccgcgctcgc cgccgacgtc ggcctcaaaa
     2281 ccgtcaccgg catcaacgtc gacaccgaaa agggcggcgt ggtgacgctg aagggcaaag
     2341 tgaatagcgc cgacatcaag aagcacgccg gagaggtggc gaagaaggtg aacggcgtca
     2401 agtcggtgaa gaaccagctg caggtcaaca agggataaat aggagacgtt ccatgaaagc
     2461 caaagcacta gccgttctga ttcccgcgct gttccttgcc accgccgcgc tcgcgcagag
     2521 caccaccacg ccgcgcgagc cggccacccg cgagccgtcg aaagaggcgg cgccgaatga
     2581 cgccaagggt agcggcagcg taagccgcga agacatgtcc gacgcgacca tcactgccaa
     2641 ggtgaagagc gcgctcgccg cagacgtggg cctgaagacc gtgaccggca tcaacgtcga
     2701 caccgacaac ggcggcgtgg ttacgctcaa gggcaaggtc gactccgccg acatgaagca
     2761 gcgcgccgag caggtggcga agaaggtgaa cggcgtgaag tcggtgaaga acgagctgca
     2821 ggtcacgaaa ggctgaagca atggccgcgg gcgtgccgct gctgtcggtc gcccggagcc
     2881 gcgacggcgt ctggagcgtg aacgagcgcg gaccggaccg cgtggccatc gcgtacttcc
     2941 ccagccagtg gggcgcgatg aaacacgcgg tgcgggtcgc gcgcgccaag cgccgctgcc
     3001 gcgttgcggt ccacgccgcc gacggcgggg tgcggctcag ccgcgactat tcctccgcgc
     3061 ccggcgcggt ggaaacgcca tgagcagcgg cgcattcgcg ctgatcgccg gggtggttta
     3121 cacgctgctc ggcatcgccg ggctgtttcc tgtcgcgggt ggctatctgg gattgttccc
     3181 gatgaacgtc ccgctcggcg ccctgcatct cgcggtcggc gtctgcggac tggccgccgc
     3241 cagcgtgcag cgctatgggc gcctgtatgc gcgttgcgtc gccgcgattc tcggcgtgct
     3301 cggcgcaatg ggcctggtcg cggggctgga cggcgccttc ggtctcatgc cgctgcacgg
     3361 ccacaacgtc tggctgcacc tgggcaccgc cgctatcgca gcctacttcg gctggcgccc
     3421 gcagattccg gtcctgcgcg aggagctcga ggcgcaggat cgccgcgatg ggttcggcga
     3481 ccggcgtgaa accattgcca cggtgtcgcg cgagcgccgc cacggcgcat acgaccggcg
     3541 cacgccgctc ggagcctgag acatgagaac gcttgcagcc gccctgtcga ttctgcttct
     3601 cgccgcctgc ggcgagacct cgcgcattcc cgaagccgcc cagaccgggc cgaatccgca
     3661 gctgccgccg ccgcagtcgt cgctcgtccc cacggtcaag atcgccccgg ccaagggctg
     3721 gccggcgggc gccacgccaa aggctgcgcc ggggttcgcg gtgacggcct ttgccgatac
     3781 cctggaccac ccgcgctggg tgtacgtcct gcccaacggc gacgtgctgg tggcggagac
     3841 caatgccccg cccaagcccg aggacggcaa gggcgtgaag ggctgggcga tgaagaaatt
     3901 catggcgcgc gccggcgcga cggcgcccag cgccaaccgc atcacgctgc tgcgcgacac
     3961 cgacggcgac ggcaaggccg agacgcagac tgtgtttctc gagggcctga aatcgccctt
     4021 cggcatggcg ctggtgggaa acgatctgta tgtggccaat accgacgcag tgatgaagtt
     4081 caaatacaag gaaggacaga cccgaatcga cgcgccgggc gagaaggtcg ccgacctgcc
     4141 cggcggcccg ctcaaccacc actggacgaa gaacatcatc gccagccccg acggcaagcg
     4201 cctctatgcg accgtgggct ccaacagcaa tgcagccgag aacggcatcg acaaggaaga
     4261 gcgccgcgcc gcgatcctcg agatcgaccc ggcgagcggc gcgacgcggc tgttcgcctc
     4321 cggcctgcgc aacccgaacg gcatgggctg gcagccgcac gacggcaggc tgtggacggt
     4381 ggtgaacgag cgcgacgagc tgggcagcga cctggtgccc gactatctca cctcggtcaa
     4441 ggacggcgcc ttctatggct ggccctacag ctactacggc cgcaacgtcg acaagcgcgt
     4501 gaagccgcag cgccccgacc tggtggacaa ggccatcgtc cccgactacg cgctgggctc
     4561 gcatgtggca ccgttggggt tggtctttta tgacaaaacc ctctttccgg aacactttgc
     4621 aggcggcgcc ttcatcggcc agcacggctc ctggaaccgc aagccgctga gcggctaccg
     4681 cgtcgtgttc gtgccattca aggacggcag gccctcttcg cccgcgccgg tggacatcct
     4741 caccgggttc gtcgacgccg acggcaaggc gatgggccgc ccggtcggcg tcgcggtcga
     4801 cggcaaaggc gccctgttgg tggccgacga cgtcggcaac atggtctggc gggtcacgcc
     4861 ggcgccctag ccggaatagt tcttgcggca gggagggcgt ggccaaacag gagggtttat
     4921 ccatgctagt caacgaagcg atgagccgcg acgtccgcgt cgccaatccc cagcagcgca
     4981 tcagcgaggc cgccaagacg atggccgaga tcgacgtcgg cgtgctgccg gtgggcgaga
     5041 acgaccgcct ggtcggcatg atcaccgacc gcgacatcgc ggtgcgcgcc gtcgccgcgg
     5101 gcaagggccc cgacaccctg gtgcgcgaag tgatgaccgt cgacgtgaag tactgcttcg
     5161 aggacgagga cctggtgcac gtctcgaaga acatggccga gcagcaggtg cgccggctgc
     5221 cggtggtcaa ccgcgaaaag cgactggtcg ggatcctcgc gctctccgac atcgcggcga
     5281 aggaaggctc gcgcgtcacc gggcgtgcga tcgagggcat ctcgcggccc ggcgggcagc
     5341 acacctccta gcgccgggga agcttcccac tgtaggctag ccgtcgagga ggagttcgat
     5401 ggcaagcgag cggccgccgt tccgcgccga ccatgtcggc agtctcctgc gtccgccgga
     5461 gctgaagcag gctcgcgcgg atctcaaggc aggaaagatt tctcgtcaat ccctgacgga
     5521 aatcgaaaac caatccatca gaagcgctgt tgcgctgcag gaagcggccg gcctgcaggc
     5581 ggtcaccgac ggcgagttcc gccgcgcctt ctggcacgtc gacttcctta ccggcttcga
     5641 gggcatcgcc gccacgcagg ggcagtacgc gctcaagttc cacggcgaag gcggcgccga
     5701 gtccgagacc aagtcgatga tggtggtcac cggcaaggtt aagcgcgcca agcccatcat
     5761 ggtttcgcac tttgaatttt tgcggaaagc aacttcgaag accgcgaagc tctgcatacc
     5821 ggcccctacg tatctccata tgcgcggcgg gcgcaaggtg gtcgacgcca aggcctatcc
     5881 cgacatcgag gagttctgga gcgacatcgc tggcgcttac cgcgaagaga tcgccgacct
     5941 ggcgagggcg ggctgcaagt acctgcagat agacgacgtg tccttcgcca ccctgtgcga
     6001 cgagtcgatc cgcgcccagg tgcggcgcga cggcgaggac ccggcgaagc tgccggccaa
     6061 atatgcctcg gtcatcagtt ccttgctgaa aggcaaaccg aaggacatga gcgtgaccat
     6121 gcatacctgc cgcggcaacc acgccagcat gtggatggcc gaaggcgcct acgacgcggt
     6181 cgccgaggga atctttcaga ccgacgtcga cggcttcttc ctcgaatatg acaccgagcg
     6241 cgccggcggc tttgaaccgc ttcgctttgt ccccaaggga aaaaaggtgg tcctgggact
     6301 gatttcctcg aaaaatccgc gcctggaaag cagggacgcg ctaaagaggc gcgttgagca
     6361 ggccgccaaa tacgtgccgc tcgagaatct gtgcctgtcg ccgcagtgcg gcttcgcctc
     6421 gagcgaggtg ggcaacaagc tgaccaccga cgaccagcgc cgcaagctcg agctggcggt
     6481 ggaaaccgcc agggaaatct ggggttagaa tcgaaactct ctagga
//
LOCUS       DASYJA010000063         3426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_827604, whole genome shotgun sequence.
ACCESSION   DASYJA010000063 DASYJA010000000
VERSION     DASYJA010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827604"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="VGO02_01335"
     CDS             <1..1212
                     /locus_tag="VGO02_01335"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEV7476360.1"
                     /translation="QSIRRRLALVLAALFLGFAGVGAAQAQVLSTRIWPARDYTRLTI
                     ESKDEIQYSIFSIKDPARLVLDLEVAELPPALAELHTKVAENDPYIQGLRTARNRPGV
                     VRLVLDLKTDVKPQAFTLRPIAEYGYRLVLDIYPLVPVDPLLALIEETEKRPQALPNR
                     ETQKITRLATVVIDPGHGGEDPGAIGRRGSREKDITLMIGRRLKALIDAEPNMRALLT
                     REGDYALQLGTRVEKARQVKADLFVSIHADSFVRPHARGSSVFALSDRRATSEMARAL
                     AKKQNEADLIAGVNLKGKDDYLKRTLLDLSQTASIDHSLRLGNFVLARLGGVNTLHKP
                     TVEQASFAVLTAHDMPSILVETAFISNPEEEKRLNDIAYQDKLARAILDGIRDYFSKN
                     PPRPASPLALR"
     gene            complement(1192..1827)
                     /locus_tag="VGO02_01340"
     CDS             complement(1192..1827)
                     /locus_tag="VGO02_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEV7476361.1"
                     /translation="MELLAWAWDLAVHLDVRLAEFVQLHGAWVYALLFLIVFCETGLV
                     VTPFLPGDSLLFVVGALAAVGDMNLFAVMGVLVAAALCGDNVNYWVGRWFGKKLLVKW
                     INPAHLQRTHDFYTRHGGKTIIIARFMPIVRTYVPFVAGLGAMPYGRYLAFCVAGALL
                     WVGLICTVGYFFGNIPAVKSNLTAVILVIVLLSITPGIIAWLKARSAQREG"
     gene            1869..2363
                     /locus_tag="VGO02_01345"
     CDS             1869..2363
                     /locus_tag="VGO02_01345"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEV7476362.1"
                     /translation="MSQDSPVLTNPSVERVRGALVRAGLTPQIVELPGAARTAQAAAA
                     FLGCEVAQIANSLVFRAQASDSAVLVMSSGAKRVDLAKLAEALQEPVGKADADFVRSR
                     TGFAIGGVAPVGHADVRKIFVEKSLAAQRELWAAAGHPHTVFRLTYDELMRITGGTQA
                     ELAA"
     gene            2360..>3426
                     /gene="mutL"
                     /locus_tag="VGO02_01350"
     CDS             2360..>3426
                     /gene="mutL"
                     /locus_tag="VGO02_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEV7476363.1"
                     /translation="MSPIRVLPELLVSQIAAGEVVERPASVLKELLENSVDAGARSVS
                     VSLDQGGIKRIQVEDDGEGIAAADLPLALARHATSKIASLEDLEGVATMGFRGEALAS
                     IASVSRLSIATRRQGAAHGSAVHAEGDAAGPVEPAARAPGTTVSVADLYFNTPARRKF
                     LRTEATEFGHCDEVFCRIALARPDVAFTLKHNGRVSRLLRAQPAAERVGALLGEAFCA
                     ASVPLDAPAAGLRLSGLAGTPQAATARADSQYFFVNGRFVRDRLLAHAARQAYAELLH
                     GERAPAYVLFLELDPRGVDVNVHPAKTEVRFRDSRAVHQFVRHALERALSPSAAAAPV
                     SYASISAPVFAGGQQQSFGLA"
BASE COUNT          570 a         1250 c         1128 g          478 t
ORIGIN      
        1 cagtccatcc ggcgccgcct ggctctcgtc cttgccgccc tctttctagg ctttgcaggt
       61 gtaggggccg cccaggccca ggtcctctcc acccgcatct ggccggcccg cgactacacg
      121 cgccttacga tcgagtccaa ggacgagatc cagtacagca tcttcagcat caaggacccc
      181 gcgcggttgg tgctcgacct cgaagtggcc gagctgccgc cggccctggc cgagttgcac
      241 accaaggtcg cggagaacga tccgtatatc caggggctgc gcacggcgcg caaccggccc
      301 ggcgtagtgc ggctggtgct cgacctgaag accgacgtga aaccgcaggc cttcaccctc
      361 aggccgatcg ccgaatacgg ctatcgcctg gtgctggaca tctacccgct ggtcccggtc
      421 gatcccctgc ttgctctgat cgaagagacc gagaagaggc cgcaggccct gcccaaccgc
      481 gagacgcaga agatcacccg cctcgcgacg gtggtgatcg accccgggca cggcggcgag
      541 gaccccggcg ccatcggccg gcgcggctcg cgcgagaagg acatcacgct gatgatcggc
      601 cggcggctca aggcgctgat cgacgccgag ccgaacatgc gcgccctcct cacgcgcgaa
      661 ggcgactacg cgctgcagct cggcacgcgg gtcgagaagg cgcggcaagt gaaggccgac
      721 ctcttcgtct cgatccacgc cgactcgttc gtccggccgc atgcgcgcgg ctcgtcggta
      781 ttcgcgcttt ccgaccggcg cgccacctcc gagatggcgc gcgcgctggc gaagaagcag
      841 aacgaagccg acctgatcgc cggggtcaac ctgaaaggca aggacgacta cctgaagcgc
      901 accctgctcg acctgtcgca gaccgcgtcg atcgaccaca gcctgcgcct gggcaacttc
      961 gtgctcgcgc gcctgggcgg cgtcaacact ctgcacaagc cgaccgtcga gcaggcgagc
     1021 ttcgccgtgc tcacggcgca cgacatgcct tcgatcctgg tcgagacggc gttcatctcc
     1081 aacccggaag aggagaagcg gctgaacgac atcgcctacc aggacaagct cgccagggcg
     1141 atcctcgacg gcatccgcga ctacttcagc aagaacccgc cgcgcccggc ttcacccctc
     1201 gcgctgcgct gagcgcgctt tcagccaggc gatgatgcct ggtgtgatcg acagcaggac
     1261 gatcaccagg atcaccgcag tcaggttgct cttcaccgcg gggatgttgc cgaagaagta
     1321 gcccactgtg cagatcaatc ccacccagag gagcgctccc gccacgcaga aggcgaggta
     1381 gcgaccgtac ggcatcgcgc cgaggcccgc gacgaagggc acgtaggtgc gcacgatcgg
     1441 catgaagcgg gcaatgatga tggtcttgcc gccgtgccgc gtatagaagt cgtgcgtgcg
     1501 ctgcagatgc gcgggattga tccacttgac cagcagcttc ttgccgaacc agcgcccgac
     1561 ccagtagttg acgttgtcgc cgcagagcgc ggcggcgacc aggacgccca tcaccgcgaa
     1621 gaggttcatg tcgcccacgg cggcgagcgc gccgacgacg aacagcagcg agtcccccgg
     1681 caggaaaggc gtcaccacca gcccggtttc gcagaacacg atcaggaaga gcagcgcata
     1741 gacccacgcg ccgtgcaact gcacgaactc ggcgaggcgc acgtcgagat gcaccgcgag
     1801 gtcccaggcc caggcgagga gctccatgcg ccgattgtat aatcgcccgc tcccgcgatg
     1861 tcccaacgat gtcccaagat tccccggtcc tgaccaatcc gagcgtcgag cgcgtgcgcg
     1921 gcgcgctcgt gcgcgccggc ctgacgccgc agatcgtgga gctgcccggc gccgcgcgca
     1981 cggcgcaggc cgccgcggcg ttcctaggct gcgaggtggc gcagatcgcc aattccctgg
     2041 tgttccgcgc acaggcgagc gacagcgcgg tgctcgtcat gtcctcgggc gcgaagcggg
     2101 tcgacctggc gaagcttgcc gaagccttgc aggagccggt cggcaaggcc gacgccgatt
     2161 tcgtgcgcag ccgcaccggc ttcgccatcg gcggcgtcgc gccggtcggg cacgcggacg
     2221 taagaaagat cttcgtcgag aaaagcctcg cggcgcagcg cgagctttgg gcggcggccg
     2281 gacatccgca taccgtcttt cgcctgacct acgacgagct catgcgcatt accggcggca
     2341 cgcaggccga gctcgccgca tgagcccgat ccgcgtcctg cccgagctcc tggtgagcca
     2401 gatcgccgcc ggcgaggtgg tcgagcggcc agcctcggtc ctcaaggaac tgctggagaa
     2461 cagcgtggac gccggcgccc gctcggtgag cgtcagcctc gaccagggcg gcatcaagcg
     2521 catccaggtc gaggacgacg gcgaaggcat cgccgccgcg gacctgccgc tcgcgctcgc
     2581 gcgccacgcc accagcaaga tcgccagcct ggaggacctc gagggcgtcg ccaccatggg
     2641 attccgcggc gaggcgctcg cctcgatcgc ctcggtttcg cgcctgtcga tcgccacgcg
     2701 gcgccagggc gcggcgcacg gcagcgccgt gcacgccgag ggcgatgccg ccgggccggt
     2761 cgagccggcg gcgcgcgcgc cgggcaccac ggtgagcgtc gccgacctct acttcaacac
     2821 cccggcgcgc cgcaagttcc tgcgcaccga ggcgaccgag ttcggccact gcgacgaggt
     2881 cttctgccgc atcgcgctcg cgcgcccgga tgtcgcgttc acgctcaagc acaacggccg
     2941 cgtcagccgg ctgctgcgcg cgcaaccggc ggcagagcgc gtcggcgcgc tcctgggcga
     3001 ggcgttctgc gccgccagcg tgccgctcga cgcgccggcc gccggcctca ggctctccgg
     3061 cctcgccggc acgccgcagg cggccaccgc gcgcgccgac tcgcagtatt tcttcgtcaa
     3121 cgggcgcttc gtgcgcgacc gcctgctcgc ccacgccgcg cgccaggcgt acgccgagct
     3181 gctgcacggc gagcgcgcgc cggcctacgt cctgttcctc gagctcgatc cgcgcggcgt
     3241 ggacgtcaac gtgcacccgg ccaagaccga agtacgcttc cgcgactcgc gcgccgtgca
     3301 ccagttcgtg cgccatgcgc tcgagcgcgc gctctcgccg agcgcggccg cggcaccggt
     3361 cagttatgca agcatttctg cccctgtatt cgcaggggga cagcaacaat cctttggact
     3421 cgccca
//
LOCUS       DASYJA010000064         7498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1281682, whole genome shotgun sequence.
ACCESSION   DASYJA010000064 DASYJA010000000
VERSION     DASYJA010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7498
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281682"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..727)
                     /locus_tag="VGO02_01355"
                     /pseudo
     CDS             complement(17..727)
                     /locus_tag="VGO02_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806774.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            629..1318
                     /locus_tag="VGO02_01360"
                     /pseudo
     CDS             629..1318
                     /locus_tag="VGO02_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586837.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(1450..1623)
                     /locus_tag="VGO02_01365"
     CDS             complement(1450..1623)
                     /locus_tag="VGO02_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEV7476364.1"
                     /translation="MLNCKEATRLVSQGLDRRLGLAERLALRLHLLICDGCTNFSRQA
                     AFLRRALSRLADR"
     gene            1750..2199
                     /locus_tag="VGO02_01370"
     CDS             1750..2199
                     /locus_tag="VGO02_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004529498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEV7476365.1"
                     /translation="MANVTRFDPRSDYFGELVDDLFKGFLVRPLGFESRGEVGAQLPR
                     AKVDVSEKNGAYLVTAELPGVKKEDIHVAIDGPQVTLEAEVKREKEASKDERTLHSER
                     VYGKVVRSFTLPQEVDESKVEAKFKDGVLELTLPKKAAAQRKQITIQ"
     gene            2286..3176
                     /gene="argB"
                     /locus_tag="VGO02_01375"
     CDS             2286..3176
                     /gene="argB"
                     /locus_tag="VGO02_01375"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEV7476366.1"
                     /translation="MNAPFNHSPQAAEKARILHEALPYIQRFHGKTIVVKFGGNAMTD
                     EALKAGFARDVVLLKLVGMNPVVVHGGGPQIEDMLTRLGKKGEFVQGMRVTDRETMDV
                     VEMVLGGQVNKEVVELINQAGGKAVGLTGQDGALIRARKMLLPSKENGKVDIGQVGEI
                     ESIDAAIIGTLEQGGFIPVVAPIATGADGTTYNINADLVAGKLAEVLRAEKLVVLTNS
                     PGVLDKDGKLLTGLTPRRIDDLVAKGVISGGMLPKIGSAVDAARNGVKAVHIIDGRVP
                     NALLLEVLTDQGVGTLIKSK"
     gene            3176..3832
                     /locus_tag="VGO02_01380"
     CDS             3176..3832
                     /locus_tag="VGO02_01380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEV7476367.1"
                     /translation="MRIARLAPSRRVWIFDLDNTLHDATARIFPAMHDQINAFLRREF
                     DVDEAAANAMREHFWRTYGTTLEGLSRHHGVHPRRFLAETHVFPELADLVVRENALKH
                     ALERLGGTKLVFSNAPRRYVGQVLKAIGLGRYFDAVYAIEDARYRGKPALHGFHFLLR
                     KHNLDPHRCCFVDDMLENLRTAHRLGMSTVWVSPIRRRVPFVDICIASVTELPSRINP
                     "
     gene            3832..4386
                     /gene="slmA"
                     /locus_tag="VGO02_01385"
     CDS             3832..4386
                     /gene="slmA"
                     /locus_tag="VGO02_01385"
                     /inference="COORDINATES: protein motif:HMM:NF007015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HEV7476368.1"
                     /translation="MPRAKGERRLEILKALAQMLEAPKWERITTAALAEKLDVSEAAL
                     YRHFASKAQMYEGLIEFIESSVFTLANKIAEDEADGRRQAEKLVEMLLAFAEKNPGMV
                     RVMTGDALVGEHERLQTRMNQFFDRFEATLKQSLRGASDGDAAGRAAALIRYAIGSLH
                     AFAKSGFSRKPAESFSAQRRYLLA"
     gene            complement(4393..5490)
                     /gene="ugpC"
                     /locus_tag="VGO02_01390"
     CDS             complement(4393..5490)
                     /gene="ugpC"
                     /locus_tag="VGO02_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEV7476369.1"
                     /translation="MAQVTLRKLEKKYDDTPAVRGIDLDIADKEFVVLVGPSGCGKST
                     TLRMIAGLEDVTSGAVMIGGEEVNDVPPKDRDIAMVFQNYALYPHMTVYENMSFGLRL
                     KRFPREEIARRVQRAAQILDITELLGRKPRQLSGGQRQRVAMGRAIVRDPKVFLFDEP
                     LSNLDAKLRVQMRTEIKKVHQKVRTTTVYVTHDQVEAMTLADRVVVMNGGLIEQVGTP
                     AELYHAPATKFVAGFIGSPAMNLVPCALQETAGALQVKIGEVSLPVPASRTSRYQTHA
                     GKPGLLLGLRPEHIVDARGPLEPGKHAFEVVVEVAEPMGMETLVYFAAQGIDVCARVS
                     PTAGAAAGARLKLAADLNHMHLVDERTGKVL"
     gene            complement(5492..6391)
                     /locus_tag="VGO02_01395"
     CDS             complement(5492..6391)
                     /locus_tag="VGO02_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV7476370.1"
                     /translation="MAAVPMDQPAARRASMKGDRRRALVWSYVFLVIFAVFFLLPPIY
                     MLITSLKTNAEISAAGSPWWIFDPTLANYFELLTSPTYLRFFRNSAVVSVLVVLITMA
                     VSIPAAFALSRMKFWGSATLATGVFLTYLVPETLLFIPLFKMFVLVNDLTGIEMINQW
                     WVLVVLYPTLTVPFCTWIMIGYFASIPKELDEAALIDGAGYLQILLKIFIPVALPGII
                     AATIFAFTVSWAQFLYPLAFTTSADQLVLPVGIITTLIRGDVFTWGQIMTGALLGAAP
                     PLVIYAFLMDYYIAGLTAGATKG"
     gene            complement(6392..7270)
                     /locus_tag="VGO02_01400"
     CDS             complement(6392..7270)
                     /locus_tag="VGO02_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV7476371.1"
                     /translation="MTLRKLVRRKAVIAFCMALPLIVIIAGLVLYPALYSIHLAMLNK
                     GMERFVGLDNFTFLFTRDTFWMVVRQSVVFAVSAVVFKALIGFCVAHFVHNVPAKGQR
                     KWRGMLLVPWVIPPAMSTLAWLWLFDPSYSAFNWLFEQVGIERVNWLGEANWARFAVI
                     LVNVWFGAPFFMIMYLAALKSVPEQLYEAASIDGANWWQKLWYITLPMMRNIIAITVL
                     FSLIVTFANFDIVRVLTAGGPLDQTHVFATWAFKLGIEGNDLPLGAAVSLFMFPILAI
                     AATFILRDISRRGNEA"
     gene            complement(7277..>7498)
                     /locus_tag="VGO02_01405"
     CDS             complement(7277..>7498)
                     /locus_tag="VGO02_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476372.1"
                     /translation="TWAEEGPPKGTLSHYPPKPGQILSVSAFPAPPKIAAQIYVQAVM
                     TKMVGKAVQESVDKSITWAASELEGFMRT"
BASE COUNT         1348 a         2448 c         2515 g         1187 t
ORIGIN      
        1 aagcgaaaaa cccgcctcaa agaacgtagc gcgcgaggtc ctcgtccttc gccagctcgg
       61 cgagccggcc gtctacatac gccgcatcga tcgtcacctc gccgggcccg cgcgtgccgg
      121 cgtcgaagga aatctcctcg agcagcttct ccagcaccgt gtacaggcgc cgcgcgccga
      181 tgttctcggt cttctcgttc acctggaacg cgatctcggc gatgcgccgg atcgcctcgg
      241 ggcggaactc gacgaccgtg ccctcggtgc caagtagcgc ctggtactgc ttcacctggt
      301 tcgcgtcggt cgagaccagg atgcgctcga agtcctccac cgacagcgag tcgagctcga
      361 cgcggatcgg gaagcgcccc tgcagctcgg ggatgagatc ggagggcttg gcgaggtgga
      421 aggcgcccga ggcgatgaac aggatatggt cggtcttcac catgccgtag cgcgtcgaga
      481 ccgtggttcc ctccaccagc ggcagcaggt cgcgctgcac gccctggcgc gagacgtcgg
      541 cgccgcccat ctcgccgcgg ctcgtgatct tgtcgatctc gtcgaggaac acgatgccgt
      601 tctgctcggc gttggcgagc gcgcgcgtct tcaggtcgtc gtcgttcacc aggcgggcgg
      661 cctcctcctc gatgaggatc tgcgtagcct cgcggatctt catgcggcgc aggcgctcgc
      721 caacgcggag cagaacggga tcgtgttcct cgacgagatc gacaagatca cgagccgctc
      781 cgaaatggcc ggcgccgacg tctcgcgcca gggcgtgcag cgcgacctgc tgccgctcgt
      841 ggagggcacc accgtgtcga cgcgctacgg catggtgaag accgaccacg tcctgttcat
      901 cgcatcgggg gccttccacc tcgccaagcc ctctgacctg atccccgagc tgcaggggcg
      961 ctttccgatc cgggtcgagc tcgattcgct ctcgatcgac gatttcgagc gcattctggt
     1021 ctccaccgac gcgagccagg tgaagcagta ccaggcgctg ctcggcacgg aaggagtgac
     1081 gctcgagttc cgcccggggg ccatccggcg catcgccgag atcgccttcc aggtgaacga
     1141 atccaccgag aacatcggcg cccggcgcct ctataccgtg ctggagaagc tgctcgagga
     1201 gatctccttc gacgcgggca agcgcgggcc cggcacggtc gagatcgacg cggcctacgt
     1261 cgacgcccgg ctggccgagc tttctaggaa cgaggatctg gcgcgctttg ttctttgagg
     1321 gcttcctcca ggcatcgcct gcagaggcag cccagcccgg gcaccggagc gagcgcggga
     1381 aggtcctcgc accagcagcc gggtgcgtcg gcgccgcagc tcaaggctgc gccgcaccgc
     1441 gcgcagttct tatcgatcgg caaggcgcga aagggccctg cggaggaagg ccgcctgcct
     1501 gctgaagttg gtgcagccgt cgcaaataag aagatgcagc cgcagcgcca ggcgctcggc
     1561 gaggccgagg cggcggtcga ggccctggga cacgaggcgg gtcgcctcct tgcaattgag
     1621 cagcatctga ccacctgtgg gtcgaacggg ctccgatcac cttacaaccg gctttcggga
     1681 gtttaccggg gttgaaaacc ctgcgggcgg ccctatctcc agggcacaga ctcaggaaag
     1741 gagacgcaaa tggcaaacgt tacccggttc gacccgcgca gtgattactt cggtgagctg
     1801 gtcgacgatc tgttcaaggg atttctggta cggccgctcg gcttcgaaag ccgtggcgaa
     1861 gtcggtgcgc agctgccccg cgcgaaggtg gacgtgtccg agaagaacgg cgcgtatctc
     1921 gtcaccgcgg agctgccagg ggtgaagaag gaagacatcc acgtcgccat cgatgggccg
     1981 caagtcaccc tcgaggccga ggtcaagcgc gagaaggaag cgtcgaagga cgagcgcacc
     2041 ctgcacagcg agcgcgtcta cggcaaggtc gtgcgcagct tcacgctgcc gcaggaagtc
     2101 gacgagtcca aggtggaagc caaattcaag gacggcgtgc tcgagctgac gctgcccaag
     2161 aaggcggccg cgcagcgcaa gcagatcacg atccagtaag tcgcaagtca gattcggtcg
     2221 taagcagcct ggagggctgg cgcgcaatgc gccggccctt ttgtttgtag aatcggtgct
     2281 gaacagtgaa cgcgcccttc aaccacagcc cgcaggccgc cgaaaaggcg cgcattctcc
     2341 acgaggcgct gccctacatc cagcgcttcc acggcaagac catcgtcgtg aagttcggcg
     2401 gcaatgccat gaccgacgag gcgctcaagg ccggctttgc gcgcgacgtg gtgctgctca
     2461 agctggtggg gatgaacccg gtcgtggtgc acggcggcgg gccgcagatc gaggacatgc
     2521 tcacgcgcct gggcaagaag ggcgaattcg tccagggcat gcgcgtgacc gaccgcgaga
     2581 ccatggacgt ggtcgagatg gtgctcggcg ggcaggtgaa caaggaagtg gtcgagctca
     2641 tcaaccaggc cggcggcaag gcggtcggcc tcaccgggca ggacggcgcg ctgatccgtg
     2701 cgcgcaagat gctcctgccg tcgaaggaga acggcaaggt cgacatcggc caggtgggcg
     2761 agatcgagtc gatcgacgcc gcgatcatcg gcacgctgga gcagggcgga ttcatcccgg
     2821 tggtggcgcc gatcgccacc ggtgccgacg gcaccaccta caacatcaac gccgacctcg
     2881 tggccgggaa gctcgccgag gtgctgcgcg ccgaaaagct ggtggtgctc accaattcgc
     2941 cgggggtcct cgacaaggac ggcaagctcc tcaccgggct cacgccgcgg cgcatcgacg
     3001 acctcgtggc gaagggcgtc atctcgggcg gcatgctgcc caagatcggc tcggcggtcg
     3061 acgccgcgcg caacggcgtg aaggccgtgc acatcatcga cgggcgcgtg cccaacgcgc
     3121 tgcttctcga ggtgctcacc gatcaaggtg ttggcacgct gatcaaaagc aagtagtgcg
     3181 cattgcacgc ctggcgccgt cgcggcgcgt ctggattttc gacctcgaca acacgctgca
     3241 cgacgcgacc gcgcgcatct tcccggcgat gcacgaccag atcaacgcgt tcctgcggcg
     3301 cgagttcgac gtcgacgagg ccgcagcgaa cgccatgcgc gagcatttct ggcgcaccta
     3361 cggcaccacg ctcgagggcc tctcgcgcca ccacggcgtg catccgcggc gcttcctcgc
     3421 cgagacccat gtcttccccg agctcgccga cctggtggtg cgcgagaacg ccctgaagca
     3481 cgcgctcgag cggctgggcg gcaccaagct cgtgttctcg aacgcgccgc gccgctacgt
     3541 cgggcaggtg ctgaaggcga tcgggctggg ccgctatttc gacgccgtct acgccatcga
     3601 ggacgcgcgc tatcgcggca agccggcgct gcacggcttc catttcctgc tgcgcaagca
     3661 caatctcgac ccgcaccgct gctgcttcgt cgatgacatg ctcgaaaacc tgcgcaccgc
     3721 gcatcgcctc ggcatgtcca ccgtctgggt cagtccgatt cggcggcgcg tgcctttcgt
     3781 cgatatctgt attgcctcgg tcacggaact gccaagtaga attaaccctt aatgccacgc
     3841 gccaaaggcg aacgcaggct ggagatactg aaggccctcg cgcagatgct cgaggcgcct
     3901 aaatgggagc gcattaccac cgccgcgctc gccgagaagc tcgacgtgtc ggaggccgcg
     3961 ctctaccgcc acttcgcctc caaggcgcag atgtacgaag gcctgatcga gttcatcgag
     4021 agctcggtct tcaccctcgc caacaagatc gccgaggacg aggccgacgg ccgccgccag
     4081 gccgagaagc tggtcgagat gctgctcgcc ttcgccgaga agaaccccgg catggtgcgc
     4141 gtcatgaccg gcgacgcgct ggtcggcgag cacgagcgcc tgcagacgcg catgaaccag
     4201 ttcttcgacc gcttcgaagc caccctcaag caatcgctgc ggggcgcatc cgacggcgac
     4261 gccgccggcc gagccgccgc gctcatccgc tatgccatcg gctcgctgca cgctttcgcc
     4321 aagtcggggt tctccaggaa gcccgccgag agcttttccg cgcagcggcg ctatctgctc
     4381 gcctagcagc gttcagagca ccttgccggt gcgctcgtcg accaggtgca tgtggttgag
     4441 gtccgcagcc agcttcaggc gcgcgccggc ggccgcgccc gccgtcgggc tgacgcgcgc
     4501 gcagacgtcg atgccctgcg ccgcgaaata gaccagcgtt tccattccca tcggctcggc
     4561 cacttcgacg accacttcga acgcatgctt gcccggctcg agcgggccgc gcgcatcgac
     4621 gatgtgctcg gggcgcaggc cgagcagaag ccccggcttc ccggcatgag tctggtagcg
     4681 cgaagtgcgg ctggccggga cgggcagcga gacttcccca atcttgactt ggagtgcgcc
     4741 ggcggtttcc tgcagcgcgc agggcaccag gttcatcgcc ggcgagccga tgaagccggc
     4801 gacgaacttc gtcgccggcg cgtggtacag ctcggccggc gtgccgacct gctcgatcag
     4861 gccgccgttc atcaccacca cgcggtcggc cagcgtcatc gcctcgacct ggtcgtgggt
     4921 gacgtacacc gtcgtggtgc gcaccttctg gtgcaccttc ttgatctcgg tgcgcatctg
     4981 cacgcgcagc ttggcgtcga gattggagag cggctcgtcg aacaggaaca ccttggggtc
     5041 gcgcacgatg gcgcggccca tcgccacgcg ctggcgctgg ccgccggaga gctggcgcgg
     5101 cttgcgcccg agcagctcgg tgatgtcgag gatctgcgcc gccctctgca cgcggcgcgc
     5161 gatctcctcg cgcgggaagc gcttgaggcg caggccgaac gacatgttct cgtacaccgt
     5221 catgtgcgga tacagcgcgt agttctggaa caccatcgcg atgtcgcggt ccttcggcgg
     5281 cacgtcgttc acctcttcgc cgccgatcat cacggcgccg ctggtgacgt cctcgaggcc
     5341 ggcgatcatg cgcagcgtgg tgctcttgcc gcagccggag ggcccgacca gcacaacgaa
     5401 ctccttgtct gcgatatcga ggtcgatgcc gcgcaccgcg ggagtgtcgt cgtacttttt
     5461 ctcgagcttg cgcagcgtga cctgggccat ctcatccctt ggtggcgccg gcggtcaggc
     5521 cggcgatgta gtagtccatg aggaaggcat agatgacgag cggcggcgcc gcgccgagca
     5581 gcgcgccggt catgatctgg ccccaggtga agacgtcgcc gcggatcagg gtggtgatga
     5641 tgccaaccgg cagcaccagc tggtcggccg aggtggtgaa ggcgagcggg tagaggaact
     5701 gcgcccagga cacggtgaag gcgaagatgg tcgcggcgat gatgccgggc agcgcgaccg
     5761 ggatgaagat cttcagcagg atctgcaggt agccggcgcc gtcgatcagc gccgcctcgt
     5821 cgagctcctt cgggatgctg gcgaagtagc cgatcatgat ccaggtgcag aacggcacgg
     5881 tgagcgtcgg gtagagcacc accagcaccc accactggtt gatcatctcg atgccggtga
     5941 ggtcgttcac cagcacgaac atcttgaaca gcgggatgaa gagcagcgtc tcgggcacca
     6001 gataggtgag gaacacgccg gtcgccagcg tcgccgagcc ccagaacttc atgcgcgaca
     6061 gcgcgaacgc cgccgggatg ctgaccgcca tggtgatcag caccaccagc accgagacca
     6121 ccgccgagtt gcggaagaac ctgaggtagg tcggcgaagt cagcagctcg aagtagttcg
     6181 ccagcgtcgg gtcgaagatc caccagggcg agcccgctgc cgagatctcg gcgttggtct
     6241 tgagcgaggt gatcagcatg tatatcggcg gcaggaggaa gaagaccgcg aatatcacca
     6301 ggaacacgta cgaccagacc aacgcgcggc gtcggtcgcc cttcatgctg gcgcgccgcg
     6361 cggcgggctg gtccatgggc acggccgcca ttcaggcctc gttcccgcgg cggctgatgt
     6421 cgcgcaggat gaaggtggcg gcgatcgcca ggatcgggaa catgaacagc gacaccgcgg
     6481 cgccgagcgg caggtcgttg ccctcgatgc ccagcttgaa cgcccaggtg gcgaagacgt
     6541 gcgtctggtc gagcggcccg ccggcggtga gcacgcgcac gatgtcgaag ttggcgaagg
     6601 tgacgatgag cgagaacagc accgtgatgg cgatgatgtt gcgcatcatc ggcagcgtga
     6661 tgtaccagag tttctgccac cagttggcac cgtcgatcga cgccgcctcg tagagctgct
     6721 cgggcacgga tttgagcgcg gcgaggtaca tgatcatgaa gaacggcgcg ccgaaccaga
     6781 cgttcaccag gatcacggcg aagcgcgccc agttcgcctc gcccagccag ttgacgcgct
     6841 cgatgccgac ctgctcgaac agccagttga aggcgctgta ggaaggatcg aacagccaca
     6901 gccaggcgag cgtgctcatg gccggcggga tcacccaggg cacgagcagc atgccgcgcc
     6961 acttgcgctg ccccttggcc ggcacgttgt gcacgaagtg cgcgacgcag aagccgatca
     7021 gcgccttgaa caccaccgcg ctcaccgcga aaaccacgga ttgcctgacc accatccaga
     7081 aggtgtcgcg ggtgaacagg aaggtgaagt tatccagccc gacgaagcgc tccatcccct
     7141 tgttcagcat cgcgaggtgg atggagtaga gcgccgggta gagcaccagg ccggcgatga
     7201 tgacgatcag cggcagcgcc atgcagaagg cgatgaccgc tttgcgccgg acgagcttgc
     7261 gcagcgtcac gctacgctag gtacgcatga agccttcgag ctcggaggcg gcccaggtga
     7321 tcgacttgtc gaccgattcc tgcaccgcct tgccgaccat cttggtcatc accgcctgca
     7381 cgtagatctg cgcggcgatc ttgggcggcg ccgggaacgc cgacaccgac aggatttggc
     7441 cgggcttggg cgggtagtgc gacagcgtgc ccttgggcgg gccttcctcg gcccaggt
//
LOCUS       DASYJA010000065         5516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_918220, whole genome shotgun sequence.
ACCESSION   DASYJA010000065 DASYJA010000000
VERSION     DASYJA010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918220"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /gene="ubiA"
                     /locus_tag="VGO02_01410"
     CDS             complement(<1..744)
                     /gene="ubiA"
                     /locus_tag="VGO02_01410"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEV7476373.1"
                     /translation="MNLAHVARRLDAYERLIRLDKPIGAMLLLWPTLWAVWLASAGRV
                     RADILIIFVIGTLLMRSAGCALNDFADRAIDPHVERTRARPLAAGEIQPREALAVAAA
                     LAAAAFGLVLFLNGLAILLSVAALAIAAGYPYIKRVFPLPQLWLGVAFGWGVPMAYAA
                     IQNRLPLDCWLLLAANVFYSFAYDTEYAMVDRDDDARLGVRSSALTLGRWDVAAVMAA
                     YGAMLAILLAVGALLQLSWWYYAGLAAAAA"
     gene            complement(783..2780)
                     /gene="recG"
                     /locus_tag="VGO02_01415"
     CDS             complement(783..2780)
                     /gene="recG"
                     /locus_tag="VGO02_01415"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEV7476374.1"
                     /translation="MKLEKLGLRSRFDFVLHLPLRYEDETSLSDPQQAPPGKPVLVEA
                     RVQRAEIAYRPRRQLIVHAEGVVLRFFNFYGSQLKQFERAAAAGERVRAFGEVRGGWF
                     GAEMAHPRYRIVRAGDELSATLTPIYPTAAGVSQKRVRQAVLEALDAADLADSLPQRV
                     RSRYALGDFGDSVRLLHRPPPGTDTRALTERTHPAWRRMKFDELLAQQLSMRFAYHRR
                     RARSAPTLPAGGPLMKGFLNNLPFKLTAAQTRVMNEVLRDLREPHPMQRLLQGDVGSG
                     KTIVAGLACLAAADAGWQAAVMAPTEILTEQHWRKFNDWFAPLGVPLAWLHGGLALQE
                     KRAALRRLKSGECSIAVGTHALLQEGVEFKRLGLIVVDEQHRFGVQQRVMLRRKAEAV
                     VPHQLMMSATPIPRTLSMAHYADLDVSVIDEMPPGRLPVATKLISAARRGEVLERIRE
                     ACAEGQQAYWVCPVIEESKDADVQTAIDTHARLRKDLKRLRVELLHGRMKSAEKAAVI
                     EDFRAGRTHLLVCTTVIEVGVDVPNASLMVIESAERFGLAQLHQLRGRIGRGARESVC
                     VLLFGEPLQGNARDRLRIIFATSDGFQIAQRDLELRGPGEFIGERQSGDPLFRFADLA
                     RDADLVEAARQSAEDLIEHDPDAARVHVERWLGSRQELTHT"
     gene            complement(2777..3154)
                     /locus_tag="VGO02_01420"
     CDS             complement(2777..3154)
                     /locus_tag="VGO02_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV7476375.1"
                     /translation="MKTAIQTREAPSAIGTYSQAVRAGDALYMSGQIGLDPATMQMAE
                     GVEAQTHRVFRNLAAVADAAGMSLDQAVRMTVYLTDLAHFARVNEIMAQYVHEPYPAR
                     AAIGVAALPRGALVEIDAILHAG"
     gene            3173..3493
                     /locus_tag="VGO02_01425"
     CDS             3173..3493
                     /locus_tag="VGO02_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV7476376.1"
                     /translation="MISSLEKLIGTPRDGALLRYSLGLEYEKAGRAADAATQLREAVA
                     RDPLYSAAWKALGRVLAETAPQAALDAFRHGIEAAKQKGDRQAEKEMSVFARRIEKKL
                     GGAA"
     gene            complement(3476..>5516)
                     /locus_tag="VGO02_01430"
     CDS             complement(3476..>5516)
                     /locus_tag="VGO02_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEV7476377.1"
                     /translation="YENDPRTLGRRLLKRGYEVGAAAVYPLRVEGRAVGVFGVQHADK
                     NVFTGELAALLERLADNISFALEGFEHLGAQRVAERKVRLNALRNEGLAHLGQFALDA
                     HDLDAVFAEAVRLLRLGCDVAAVVERVGPFEFLMRAATGEGAEAAVGRAASVYPDSKW
                     PAAIERGATLVSDRAHYQSRSSDRPWSFWRRRMGSGVYVPLHGERDPYGVLCMNSLRD
                     DAFDEDDMRFAEAVASVLSGAVRRLQTQTRLAYMAEFDALTGLPNRNLLQDRLAQTVA
                     QVHRRGEQGAVLFIDLDRFKLINDTLGHHLGDALIAQVGERLKHCVRSGDTVGRVSGD
                     EFGVVLSELSRADDAAIVAQKILNALARPFDLEGNEAYVTASIGISVFPSDGEEAEVL
                     LKNADLAMYRSKEMTRNAYCFFTAEMNRRSVAKLQLYTDLRRAIERREFVLHYQPKVD
                     LREGRLLGVESLLRWNHPQRGMVMPGEFVPALEDSGLILPVGDWVLQEACAQLRRWQA
                     QRLDVVPVAVNLSAKQFLRPDLDAQVRRVLAAEGVPPRLIELEITESCLMSDPEEALR
                     LLASLRAAGLKISVDDFGTGYSSLSYLTRLPLSALKIDRSFVRDAASSREAASIVRAV
                     IDMAHNLHFTVIAEGVETDEQVAFLRRHGCDLGQGHLFGAPMPAAQLLARLSRAA"
BASE COUNT          844 a         1962 c         1925 g          785 t
ORIGIN      
        1 cgccgcagcg gccgccagcc cggcgtagta ccaccaggaa agctgcagca acgcgccgac
       61 ggccagcagg atcgccagca tcgcgccgta cgcagccatc accgccgcga catcccagcg
      121 gcccagggtg agcgccgagc tgcgcacgcc gagccgcgcg tcgtcgtcgc ggtcgaccat
      181 cgcgtactcg gtgtcgtagg cgaacgagta gaagacgttg gcggcgagca gcagccagca
      241 gtcgagcggc aggcggttct ggatcgcggc ataggccatc ggcacgcccc agccgaaagc
      301 gacccccagc cacagctgcg gcagcggaaa gacgcgcttg atgtagggat agcccgcggc
      361 gatggcgagc gcggcgaccg acagcaggat cgccaggcca ttgaggaaca gcaccaggcc
      421 gaaagcggcg gcggcgagcg ccgcggccac ggcgagcgcc tcgcgcggct ggatctcgcc
      481 ggcggcgagc gggcgggcgc gcgtgcgctc gacgtgcggg tcgatggcgc gatcggcgaa
      541 gtcgttgagc gcgcagcccg cagagcgcat gagcagcgtg ccgatgacga agatgatcag
      601 gatgtcggcg cgcacccggc cggccgaggc gagccatacc gcccatagcg tcggccacag
      661 cagcagcatc gcgccaatcg gcttgtcgag ccggatcagg cgctcgtagg cgtccagccg
      721 cctggcgacg tgagccagat tcatcgccat aggataacgc cccgggtaac cggtcgcacg
      781 cctcatgtat gagtgagctc ctgccgcgag ccgagccagc gctcgacgtg cacgcgcgcc
      841 gcgtcggggt cgtgctcgat caggtcctcg gccgactggc gcgccgcctc caccaggtcc
      901 gcatcgcgtg ccaggtcggc aaagcgaaac agcggatccc cgctttgccg ctcgccgatg
      961 aattcgccgg ggccgcgcag ctccaggtcg cgctgcgcga tctggaagcc gtcgctggtt
     1021 gcaaaaatga ttcgcagcct gtcgcgggca ttgccctgca ggggctcgcc gaacagcagg
     1081 acgcagacgc tttcgcgcgc accgcgcccg atgcggccgc gcagctggtg cagctgggca
     1141 agaccgaagc gttccgcgct ctcgatcacc atcaaggacg cgttcggcac gtcgacgccg
     1201 acctcgatca ccgtggtgca gaccaggaga tgcgtcctgc cggcgcggaa atcctcgatg
     1261 accgccgcct tctcggccga cttcatgcgc ccgtgcagca gctcgacgcg caagcgcttc
     1321 aggtccttgc gcagcctggc gtgcgtgtcg atcgcggtct gcacgtctgc gtccttcgat
     1381 tcctcgatca ccgggcagac ccagtacgcc tgctggcctt cggcgcaggc ctcgcggatg
     1441 cgttccagca cctcgccgcg ccgcgccgcc gagatgagct tggtggcgac cggcagcctg
     1501 cccggcggca tctcgtcgat caccgagacg tccaggtcgg cgtagtgggc catcgagagc
     1561 gtgcgcggga tcggcgtcgc gctcatcatc agctggtgcg gcactaccgc ctcggccttg
     1621 cgcctgagca tcacgcgctg ctgcacgccg aagcggtgct gctcgtcgac cacgatcagg
     1681 ccaagccgct tgaactcgac gccctcctgc agcagcgcat gggtgccgac cgcgatcgag
     1741 cactcgccgc tcttcagcct gcgcagcgcg gccctcttct cctgcagcgc aaggccgccg
     1801 tgcagccacg cgagcggcac gccgagcggc gcgaaccagt cgttgaactt gcgccagtgc
     1861 tgctcggtga ggatctcggt gggggccatc accgccgcct gccagccggc gtcggccgcg
     1921 gccaggcacg ccagccctgc gacgatcgtc ttgccgctgc cgacgtcgcc ctgcagcagg
     1981 cgctgcatgg ggtgcggctc gcgcagatcg cgcaggactt cattcatgac gcgcgtctgc
     2041 gccgcggtga gcttgaacgg caggttgttc aggaaccctt tcatcagcgg cccgcccgcg
     2101 ggcagcgtcg gcgcgctgcg cgcgcggcgc cggtgataag cgaagcgcat ggatagctgc
     2161 tgcgcgagca gctcgtcgaa cttcatgcgc cgccatgcgg ggtgcgtgcg ctcggtgagc
     2221 gcgcgcgtgt cggttcccgg cggcggccgg tgcagcagcc gcacgctgtc gccgaaatcg
     2281 ccgagcgcgt agcggctgcg cacccgctgc ggcaggctgt cggcgaggtc ggcggcgtcg
     2341 agcgcctcga gcaccgcctg ccgcacccgc ttctgcgaca cgccggcggc agtcggatag
     2401 atcggcgtca gggtcgccga gagctcgtcg cccgcgcgca cgatgcgata gcgcggatgc
     2461 gccatctccg cgccgaacca gccgccgcgc acctcgccga aggcgcgcac ccgctcgccg
     2521 gccgcggcgg cgcgctcgaa ctgcttcagc tggctgccgt agaaattgaa gaagcgcagc
     2581 accacgcctt cggcgtgcac gatgagctga cggcgcgggc ggtaggcgat ctcggcgcgc
     2641 tgcacgcgcg cttcgaccag caccggcttt cccggcggcg cctgctgcgg gtcgctgagc
     2701 gaagtctcgt cctcgtagcg cagcggcagg tgaagcacga agtcgaaccg cgagcgcagc
     2761 ccgagctttt cgagcttcac cccgcgtgca ggatcgcgtc gatctcgacc agcgcgccgc
     2821 gcggcagcgc cgccaccccg atcgcagcgc gcgccgggta gggctcatgc acatactgcg
     2881 ccatgatctc gttcacccgg gcgaagtgcg ccaggtcggt caggtagacg gtcatgcgca
     2941 cggcctggtc gaggctcatt cccgcggcgt cggcgactgc ggcgaggttg cggaacacgc
     3001 ggtgcgtctg tgcctccacg ccctcggcca tctgcatggt cgccgggtcc aagccgatct
     3061 gccccgacat atacaaagcg tcgcccgcgc gcaccgcctg ggagtaggtg ccaatcgccg
     3121 agggcgcctc gcgcgtctgg atcgctgtct tcatgccgcg tatcttatcg tagtgatttc
     3181 cagcctggag aagttgatcg gcacgccgcg cgacggcgcg ctgctgcgct actccctcgg
     3241 cctcgagtac gagaaggcgg gtcgcgccgc cgacgccgcg acgcagctgc gggaggctgt
     3301 tgcccgcgac ccgctctatt cggccgcctg gaaagccctc ggcagggtcc tggccgagac
     3361 cgcgccgcag gcggcgctcg atgccttccg ccacggcatc gaggcggcaa agcagaaagg
     3421 cgaccgtcag gccgagaagg agatgagcgt cttcgccagg cggatcgaga agaagctagg
     3481 cggcgcggct tagccgcgcc agcagctgcg cggccggcat cggcgcgccg aagagatggc
     3541 cctggccgag gtcgcaacca tggcggcgca ggaaggctac ctgctcgtcg gtctcgacgc
     3601 cctccgcgat cacggtgaaa tgcagattgt gcgccatgtc gatgaccgcg cgcacgatcg
     3661 atgccgcctc gcggctcgac gcggcatcgc gcacgaagct gcggtcgatc ttgagggcgc
     3721 tgagcggcag ccgcgtcagg tacgagaggc tggagtagcc ggtgccgaag tcgtcgaccg
     3781 agatcttcag gccggcggcg cgcaggctgg cgagcaggcg cagcgcttcc tccggatccg
     3841 acatcaggca gctctcggtg atctcgagct cgatcaggcg gggcggcacg ccctcggcgg
     3901 cgagcacgcg ccgcacctgc gcatccagat cggggcgcag gaactgcttg gccgaaaggt
     3961 tgaccgccac cggcaccacg tccaggcgct gcgcctgcca gcggcgcagc tgcgcgcacg
     4021 cttcctgcag cacccagtcg cccaccggca ggatcagccc ggagtcctcg agcgcgggga
     4081 cgaattcgcc cggcatcacc atgccgcgct gcggatggtt ccagcgcagc aggctctcga
     4141 cgccgagcag gcggccctcg cgcaggtcga ccttcggctg gtagtgcagc acgaactcgc
     4201 gccgctcgat ggcgcggcgc aggtcggtgt agagctgcag cttcgccacc gagcggcggt
     4261 tcatctcggc ggtgaagaag cagtaggcgt tgcgcgtcat ctccttggag cggtacatcg
     4321 cgaggtcggc gttcttgagc agcacctcgg cctcctcgcc gtcggacggg aacaccgaga
     4381 tgccgatgct cgccgtgacg taggcctcgt tgccttccag gtcgaacggc cgggcgagcg
     4441 cgttgaggat cttctgcgcc acgatcgcgg cgtcgtcggc gcgcgaaagc tcggacagca
     4501 ccacaccgaa ctcgtcgccg ctcacccgcc cgacggtatc gccgctgcgc acgcagtgct
     4561 tgaggcgctc gccgacctgc gcgatcagcg cgtcgccgag gtggtggccg agcgtgtcgt
     4621 tgatcagctt gaagcggtcg aggtcgatga acagcaccgc gccctgctcg ccgcgccggt
     4681 gcacctgcgc cacggtctgc gccagccggt cctgcagcag gttgcggttc ggcaggccgg
     4741 tgagcgcgtc gaattccgcc atgtaggcga ggcgcgtctg cgtctgcagc cggcgcaccg
     4801 cgccagagag cacgctcgca acggcctcgg cgaagcgcat gtcgtcctcg tcgaaagcgt
     4861 cgtcgcgcag ggagttcatg cacagcacgc cgtatgggtc gcgctcgccg tgcagcggga
     4921 cgtagacgcc gctgcccatc cgtcggcgcc agaacgacca cgggcggtcg gagctgcggg
     4981 actggtagtg cgcgcggtcg ctcaccagcg tcgcgccgcg ctcgatggca gccggccact
     5041 tcgaatccgg gtagacgctg gccgcacggc cgactgcggc ttccgcgccc tcgcccgtgg
     5101 ccgcgcgcat caggaactcg aacggcccta cgcgctccac cacggccgcc acgtcgcagc
     5161 ccaggcgcag cagtcgcacc gcctcggcga acaccgcatc caggtcgtgg gcgtccagcg
     5221 cgaactggcc cagatgcgcg aggccttcgt tgcgcagcgc attgagccgg accttgcgct
     5281 cagcgacgcg ctgcgcgccg aggtgctcga agccctcgag cgcgaaggag atgttgtcgg
     5341 cgaggcgctc cagtagcgcc gccagttcgc cggtgaacac gttcttgtcg gcgtgctgca
     5401 cgccgaacac gccgaccgcc ctgccttcga cgcgcagcgg atagaccgcg gccgcgccca
     5461 cctcgtagcc gcgcttgagc aggcggcggc ccagcgtgcg cgggtcgttc tcatag
//
LOCUS       DASYJA010000066         2112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_101240, whole genome shotgun sequence.
ACCESSION   DASYJA010000066 DASYJA010000000
VERSION     DASYJA010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101240"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /gene="mnmH"
                     /locus_tag="VGO02_01435"
     CDS             <1..702
                     /gene="mnmH"
                     /locus_tag="VGO02_01435"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008752.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HEV7476378.1"
                     /translation="QGGYKAYRRWVVAQLEEIPSRIRFTVVHGPTGSGKSRLLAALAR
                     SGGQVLDLEALAAHRGSVLGGLPGQPQPSQKMFESRLLAALESFDAARPVFVEGESKK
                     IGELQVPDALMAAMRASPCVRLEASVETRVALLLDEYRHFILDRSALDAQLDCLAALH
                     GRERIAEWKALAAGGAWREFVARLLVEHYDPAYQRSSHRNFAQLSEARQVRIASAEEA
                     SFMQATEALLEVVAA"
     gene            699..1133
                     /locus_tag="VGO02_01440"
     CDS             699..1133
                     /locus_tag="VGO02_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476379.1"
                     /translation="MNPLAAAVLAAGELICEFNDGYRKSLIAEIAGDTPRVELVLVYE
                     ALRADSAEVLSTRRPGRRAVKVRASGEQLHLIEQDGPSVRVTTLTACTRTKLRPEGEV
                     CTRFDARHAWHFDSTAALDPQAAAARLPSGAATGSCEPWKIE"
     gene            1153..>2112
                     /locus_tag="VGO02_01445"
     CDS             1153..>2112
                     /locus_tag="VGO02_01445"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEV7476380.1"
                     /translation="MQIPRGDNLRNDIIGGLVSAGVAIPLAMGYGMFAFIALGNEYFP
                     DGALAGLVTAVIVGIVCVALGDKSSNLYAPRVTTTFFIGILLYNLVHADVPIIKSGGL
                     GVIIPVIFSIILLAGVFQALFGLARLGTLIKHTPQPVMAGFQNAAALLLILVQMANIF
                     GFDKSTSFIHALKDAPHAKPLSVAIAIAAVVAMWNSKRFLPKIPPLAVGLAVGTVLYY
                     AFGLAGFGLQLGPTIGSGPFNSYKLPNFPQFGDLARTPGIVSLLPTIVGGALALAIIA
                     SIDALLCAKILARPGEPKANSDRLLMRLGAANALAAGAGGITGG"
BASE COUNT          313 a          778 c          680 g          341 t
ORIGIN      
        1 cagggcggct acaaggccta ccgcaggtgg gtggtcgccc agctcgaaga aataccttcg
       61 agaataagat ttacggtcgt gcacggaccg accggcagcg gcaagagccg cctgctggcg
      121 gccctcgccc gctcgggcgg ccaggttctg gatctcgagg cgctcgccgc gcaccgcggc
      181 tcggtgctcg gcgggctgcc cggccagccg cagccttcgc agaagatgtt cgagagccgg
      241 ctgctcgccg cgctcgagtc cttcgacgcc gcgcgcccgg tgttcgtcga gggcgagtcg
      301 aagaagatcg gcgagctgca ggtgcccgat gcgctgatgg cggcgatgcg cgcctcgccc
      361 tgcgtgcgcc tagaagcgag cgtcgagacc cgcgtcgctc tcctgctcga cgaataccgc
      421 cacttcatcc tcgaccggtc cgcgctcgac gcccagctcg actgcctggc ggcgctgcac
      481 ggccgcgagc gcatcgccga atggaaggcg ctcgcggcag gcggcgcgtg gcgcgagttc
      541 gtcgcgcgtc tcctcgtcga gcactacgac cccgcctacc agcgctcctc gcaccgcaac
      601 ttcgcgcagt tgtccgaagc gcggcaggtg cgcatcgcat cggccgagga agcctccttc
      661 atgcaggcca ccgaagccct gctcgaggtg gttgccgcgt gaacccgctg gccgccgccg
      721 tgctcgccgc gggcgagctg atctgcgagt tcaacgacgg ctaccgcaag agcctgatcg
      781 ccgagatcgc cggcgacacg ccgcgcgtcg agctggtgct ggtgtacgaa gcgctgcgcg
      841 cggattccgc cgaagtgctc tccactcgcc gccccggccg ccgcgcggtg aaggtacgcg
      901 ccagcggcga gcagcttcat ctcatcgagc aggacggccc cagcgtgcgc gtcaccacgc
      961 tcaccgcctg cacgcgcacc aagctgcgcc cagagggcga ggtctgcacc aggttcgacg
     1021 cgcgccacgc ctggcacttc gacagcaccg cggccctgga tccgcaggcc gccgccgctc
     1081 gcctgccctc cggcgccgcc accggcagct gcgagccgtg gaagatcgaa taatcatgta
     1141 acctcgtcgc gaatgcagat cccaaggggg gacaacctgc gcaacgacat catcggcgga
     1201 ctcgtctccg ccggcgtcgc cattcccctg gcgatgggct acggcatgtt cgccttcatc
     1261 gcgttgggga acgagtactt ccccgacggg gcgctggccg gactggtcac cgccgtgatc
     1321 gtcggaatcg tctgcgtcgc gctgggcgac aagtcgtcga atctgtacgc gccgcgggtc
     1381 accaccacgt tcttcatcgg catcctgctc tacaacctgg tgcatgccga tgtgccgatc
     1441 atcaagtccg gcggactggg ggtgatcatt ccggtcatct tctcgatcat tctcctggcc
     1501 ggcgtgttcc aggcgctgtt cggcctggcg cggctcggca cgctgatcaa gcacaccccg
     1561 cagccggtga tggcgggctt ccagaacgcc gccgcgctcc tcctgatcct cgtgcagatg
     1621 gcaaatatct tcggcttcga caagagcacg tcgttcatcc atgcgctgaa ggacgcgccc
     1681 cacgccaagc cgctcagcgt cgccatcgcg atcgcggccg tggtggccat gtggaactcg
     1741 aaacggtttc tgccgaaaat cccgccgctg gccgtgggcc tggccgtcgg cactgtcctc
     1801 tactacgcct tcgggctggc cggcttcggc ctgcagctcg gcccgacgat cggcagcggg
     1861 cctttcaatt cgtacaagct gcccaatttc ccgcagttcg gcgacctggc gcgcaccccc
     1921 gggatcgtct cgctcctgcc cacgatcgtc ggcggcgcgc tcgcgctcgc catcatcgcc
     1981 tcgatcgacg ccctgctttg cgccaagatc ctggcccggc ccggcgagcc caaggccaac
     2041 agcgatcgcc tgctcatgcg cctgggcgcc gccaacgcgc tggccgccgg cgccggaggc
     2101 atcacgggcg gc
//
LOCUS       DASYJA010000067         2529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1645021, whole genome shotgun sequence.
ACCESSION   DASYJA010000067 DASYJA010000000
VERSION     DASYJA010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645021"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VGO02_01450"
     CDS             <1..993
                     /locus_tag="VGO02_01450"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEV7476381.1"
                     /translation="HFFFKLLGIVMFVLELFWFIFRPIWKEAGYLWQARKAVRMPWRP
                     AVAVLAVAGVAVWVIPVSHEVTAPAILRAQHEHAVYAPFPARITEVRVAELQNVAAGT
                     ELLRLEAAELGVREKKADIGIASARAELARMPANIQLQENFQVLQQRLAQAVAERQAV
                     TEEFNRQQLRSAQEGTIRDVAPDLVVGRWVSPNQLLMRVVSQSEPVIEAFVSEQQVFA
                     LAPGQTVRFFPRLADRPVLEGRVVAVDRAPQKELQRPLLASIYGGDVLVKQGQRGALI
                     AQDAVYRVMIKPIGNIPKADAVIAGRVRIETSFRFLAENFAYRIISLFIRESGI"
     gene            1015..>2529
                     /locus_tag="VGO02_01455"
     CDS             1015..>2529
                     /locus_tag="VGO02_01455"
                     /inference="COORDINATES: protein motif:HMM:NF025611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4347 domain-containing protein"
                     /protein_id="HEV7476382.1"
                     /translation="MAKNSKTPTSSAKKPKTRAALRRRTRMIALEPRMLFDGALGIDL
                     GAKGTAALRGDTSFDAGQAPTPAAPEAQRTDAVGEKPAEKATERAAEKTIESLDSRPD
                     AQPKELVFVDARAEGAQALLKNVDPNARVVILDPARNGIQQIVDALAGESDVSAIHIV
                     AMGTSERLLLGGSSLDASSMQGLNAQRLSSISEHLTSDASILIHGADFGKGDAGSAVA
                     NRLALLTSADVADVSDDGTVERVGTPARQEIVFVDPNVRDFQALVDGIDNPNARVVLL
                     DGTRDGVQQIADVVKQYQRVDAIHIISHGSEGQIDLAGSALNAATMSGQYAAQLAEIG
                     KHLGAEADVLIYGCNFGKGADGAAAANELAQLTGADVAASIDDTGDAALGGNWQLEYR
                     TGAVDSDIALDRLAQDSWHDVMALHTLDFDTAPAWTAGASNTIYTIDGYPVTISVSPA
                     TNGDPVQSNSYTGGVTPAQNALQFTLGATASNTMTIDFSGQPGGAVGNVGFALWD"
BASE COUNT          448 a          854 c          852 g          375 t
ORIGIN      
        1 cacttcttct tcaagctgct cggcatcgtg atgttcgtgc tcgagctgtt ctggttcatc
       61 ttccggccga tctggaaaga agctggctac ctgtggcagg cgcgcaaggc cgtgcgcatg
      121 ccttggcgcc cggcggtcgc ggtgctggcg gtggcgggcg tagcggtgtg ggtgatcccg
      181 gtttcccacg aagtgaccgc gcccgccatc ctgcgcgcgc agcacgagca cgccgtctat
      241 gcgcctttcc cggcgaggat caccgaggta cgcgtcgccg agctgcagaa cgtggcggcg
      301 ggcacggagc ttctgcgcct ggaagccgcc gaactgggcg tgcgcgagaa gaaggccgat
      361 atcgggatcg cgtcggcgcg cgccgagctg gcgcgcatgc cggccaacat ccagctgcag
      421 gagaactttc aagtgctgca gcagcggctg gcgcaagccg tagccgagag gcaggcggtc
      481 accgaggagt tcaatcgcca gcagctgcgc tcggcgcagg agggaaccat ccgggacgtc
      541 gcgcccgacc tggtggtggg ccgctgggtc agcccgaacc agctgctgat gcgcgtggtc
      601 tcgcaatccg agccggtgat cgaagctttt gtgagcgagc aacaggtctt cgccctggct
      661 ccggggcaga cggtgcgctt cttcccgcgc ctggcggatc ggccggtgct ggaaggccgc
      721 gtggttgcag tcgacagggc gccgcaaaag gagctgcagc ggccgctgct cgcttcgatc
      781 tacggcggcg atgtgctcgt caagcagggc cagcgcggcg cgctcatcgc gcaggatgcg
      841 gtctaccgcg tcatgatcaa gccgatcggc aatattccga aagccgatgc ggtgatcgcc
      901 ggccgcgtca ggatcgaaac cagtttccgc ttcctggcgg aaaacttcgc ctaccgcatc
      961 atctcgctgt tcatacggga aagcggcatc tgattccgcc gcgcgacaaa aaccatggcc
     1021 aagaactcca agacacccac tagctccgcg aagaagccca agacccgggc cgcattgcga
     1081 cggcgcacgc gcatgatcgc gctcgagccg cgcatgctgt tcgacggcgc gctcggcatc
     1141 gacctcggcg ccaagggcac ggccgcgctg cgcggcgaca ccagcttcga cgccggccag
     1201 gcgccgacgc cggcggctcc ggaggcccag cgcaccgatg cggtcggcga gaagccggcc
     1261 gagaaagcga ccgagcgggc cgccgagaag accatcgagt cgctcgatag ccggcccgac
     1321 gcgcagccca aggagctggt cttcgtcgat gcccgcgccg aaggcgccca ggcgctgctc
     1381 aagaacgtcg acccgaacgc cagggtcgtg atcctcgatc cggcgcgcaa cggcatccag
     1441 cagatcgtcg acgcgctcgc cggcgagagc gacgtgagcg cgatccacat cgtcgcgatg
     1501 ggcactagcg agcggctcct gctcggcggc agctcgctcg acgcgagctc gatgcaaggg
     1561 ctgaacgcgc agcgcctgtc ctcgatcagc gagcacctca cctccgacgc ttcgatcctg
     1621 attcacggtg ccgatttcgg caaaggcgat gccggcagcg cagtggccaa ccgcctggcg
     1681 ctgctgacca gcgcggacgt cgccgacgta agcgatgacg gcacggtcga gcgggttggc
     1741 actcccgcgc ggcaggaaat cgtcttcgtc gaccccaacg tgcgcgactt ccaggccctc
     1801 gtcgacggca tcgacaatcc gaatgccagg gtcgtgctgc tcgacggcac gcgcgacggc
     1861 gtccagcaga tagcggacgt ggtcaagcaa taccagcgcg tcgacgcgat ccatatcatc
     1921 tcgcacggca gcgaagggca gatcgacctc gccggcagcg cattgaacgc cgccaccatg
     1981 tccgggcagt acgcggcgca gctcgccgag atcggcaagc acctcggtgc cgaggcggac
     2041 gtgctgatct acggctgcaa tttcggcaag ggcgcagacg gcgccgcggc agctaacgag
     2101 ctggcgcaac taaccggcgc ggacgttgcc gccagcatcg acgacaccgg cgacgctgcg
     2161 cttggcggca actggcaact cgagtaccgc accggcgcgg tcgactccga catcgccctc
     2221 gatcgcctgg cgcaggattc gtggcacgac gtcatggcgc tgcacacgct tgacttcgac
     2281 actgcaccgg cctggacggc gggcgcgtct aacaccatct acacgataga cggctacccg
     2341 gtgaccatca gcgtcagccc ggcgaccaat ggcgatcctg tccagtcgaa cagttatacg
     2401 ggcggcgtta ccccggcgca gaacgcattg cagttcacgc tcggtgccac cgcgtcgaac
     2461 acgatgacga tcgatttctc ggggcagccc ggcggcgcgg tgggcaacgt gggcttcgcg
     2521 ctgtgggac
//
LOCUS       DASYJA010000068         3373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1191537, whole genome shotgun sequence.
ACCESSION   DASYJA010000068 DASYJA010000000
VERSION     DASYJA010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191537"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VGO02_01460"
     CDS             complement(<1..515)
                     /locus_tag="VGO02_01460"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEV7476383.1"
                     /translation="MCALALGKQGIPVIVLESEGGLTRDLRAGSFHPATLDMMEPFGI
                     TARMDQEGIRVRYWQIRDRSGELVAQFDLGLLEGETRHPYRLHLEQHRLTPIQLELIQ
                     QATKAEVHFGYSVTGLEARDDRVLVHISGHDGPAMIEASWVIGADGGRSTVRKSLGIE
                     FEGFTWPEQFLV"
     gene            complement(583..1542)
                     /locus_tag="VGO02_01465"
     CDS             complement(583..1542)
                     /locus_tag="VGO02_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7476384.1"
                     /translation="MRFSFVLIAAALFSGSAAAQGYPSQPIKFVVPFTPGTGMDIIAR
                     QVGPRLTERLGQPVVVENRPGASGNIGAEIVARAAPDGYTIMVGANTLVIAAHLYQQV
                     PFDPLTDFAPVALAGSTTLVLVANPASGIDSVPELIARAKAQPGKLTYGSPGIATPHH
                     MAMELFKDLTGTDLLHIPYKGSAGAVTDLVSGQINLMFLPIHQVLPYRQAGRLKLLAA
                     GGATRHPSAPELPTLVELGIRGAEAADIWYAFYAPKGVPAPVIAKLNAELRSILALAE
                     VRAAVENVGVDVHTSTPDELFALMKRDYARWGEIVRKNHITAQ"
     gene            complement(1682..2479)
                     /locus_tag="VGO02_01470"
     CDS             complement(1682..2479)
                     /locus_tag="VGO02_01470"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV7476385.1"
                     /translation="MREVEKLLHLPRSTVRALVQAGFVAPNRGPRGAWQFSFQDLIVL
                     RTAQELCEAKVPRRRIARSMKELRRHLPDAMPLSGLRICAVADRVVVREGASHWQADS
                     GQYLLAFEGDPASGSLSVVEQAKTQPADAQQWLDKAAALELEDVGAALLAYERAISLD
                     PGLLEAQINRACLLHEAGRLAEAERAYRGALDTCQNAPALLYNLATVLEDLGRKEHAV
                     EAYRNALKGDPRLADCHYNLGLLYEELGRSKEAIRHMAQYRKLVKTK"
     gene            complement(2509..>3373)
                     /locus_tag="VGO02_01475"
     CDS             complement(2509..>3373)
                     /locus_tag="VGO02_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEV7476386.1"
                     /translation="SIASLTISFGMVSIPVKLYSATEASRAISFNLLHKACGSRLKQQ
                     YFCVKEEVPVAREDMAKGYEFAKDQYVMFTPEELKALEEAGTHSAEITEFVPLKTVDP
                     VYFDKAYYLGPDKGGAKPYALLARALRETGRCALGRWAARGKQYIVMIRPVEDLVEGL
                     VMQQLLYAGEVRSMRDVDIPKTEVKPAELKLAQQLIEQQSSEKFDPAAYTDEVRARIE
                     VAVQKKVEGQEITMAEAPETGAQVIDLMEALRASLGKKAAPAAKDAQPEARKPPKRAQ
                     AEPAQRKAAKK"
BASE COUNT          536 a         1143 c         1096 g          598 t
ORIGIN      
        1 accaggaact gctcgggcca ggtgaatccc tcgaattcga tgccgagcga cttgcgcacg
       61 gtgctgcgtc cgccgtcggc gccgatcacc caggaagcct cgatcatcgc cgggccgtcg
      121 tggccgctga tatgcacgag cacgcggtcg tcgcgcgctt cgagcccggt caccgaatag
      181 ccgaaatgca cctcggcttt ggttgcctgc tggatcagct cgagctggat cggcgtcagg
      241 cgatgctgct cgagatgcag acggtacgga tgccgcgtct cgccttcgag caggccgagg
      301 tcgaactgcg ccaccagctc gccgctgcgg tcgcggatct gccagtagcg aacgcgtatg
      361 ccttcctggt ccatccgcgc ggtgatgccg aaaggctcca tcatgtccag cgtggccggg
      421 tggaacgatc cggcgcgcag gtcgcgcgtc agcccgccct cggattccag gacgatgacc
      481 gggatgccct gcttgccaag cgcgagcgcg cacatcagcc cggtggggcc ggcgcccgct
      541 atgacgactg gcagttgatc gcggcttatg tgctgtctcc ggctattggg cagtgatgtg
      601 gttctttctg acgatctcgc cccagcgcgc gtaatcgcgc ttcatcagag cgaacagctc
      661 atccggcgtt gaggtgtgca cgtcgacgcc gacgttctcc accgcagccc tgacttcggc
      721 aagcgcgagg atcgagcgca actcggcgtt gagcttcgcg atcaccggcg cgggcacgcc
      781 cttgggtgca tagaacgcat accagatgtc ggcggcttcg gcgccgcgaa tgccgagctc
      841 gaccagcgtg ggcagctcgg gggcggaggg atggcgcgtg gcgccgcccg cagccagcag
      901 cttgagcctg cccgcctgcc ggtaaggcag gacctggtgg atcggcagga acatcaggtt
      961 gatctggccg gaaaccaggt cggtgacagc gccggcgctg cccttgtacg gaatgtgcaa
     1021 caggtcggtt ccggtcagat ccttgaacag ctccatcgcc atgtgatgcg gcgtggcgat
     1081 gcccggcgag ccgtaggtga gcttgcccgg ctgcgccttc gcccgagcga tgagctccgg
     1141 cacgctgtcg atgccggacg ccgggttggc caccaggacc agcgtagtgg aacccgccag
     1201 cgccacgggg gcgaagtcgg taagcggatc gaaaggcacc tgctggtaca gatgggccgc
     1261 gatcaccagg gtgttcgcgc ccaccatgat cgtgtacccg tcgggcgcgg ctctggcgac
     1321 gatctcggcg ccgatattgc cgctcgcccc tggacggttc tccaccacca cgggctgccc
     1381 cagccgctcg gtgagccgcg ggcccacctg gcgcgcgatg atgtccatgc ccgttcccgg
     1441 cgtaaacgga acgacgaact tgatgggttg cgaaggatag ccctgggctg ccgccgaccc
     1501 gctgaaaagg gcagccgcaa tcagcacgaa cgaaaaacgc atcccgccat cctcccgtga
     1561 gtctttatgc tccggggcgt gtcgagccct cgtcaaaaaa gacgaaaact cgtcaggttt
     1621 taggccttaa caggccccac gccccccaac gacgggtaaa cagcaagacg cgggccaccg
     1681 attacttggt tttgaccagc ttgcggtatt gggccatatg gcgaatggcc tccttggatc
     1741 ggcccagctc ctcgtacagg aggccgaggt tgtagtggca atccgccagg cgcggatcgc
     1801 ccttcagcgc gttccggtag gcttccaccg cgtgctcctt gcgcccgagg tcctccagta
     1861 ccgtggccag gttgtagagg agcgccgggg cattttggca agtatccagc gcgccgcggt
     1921 aggcgcgctc cgcttcggcg agccgccccg cttcatgcag caggcaagcg cgattgattt
     1981 gcgcttcgag caggcccggg tcgagcgata tagcccgctc gtacgcaagc agggccgccc
     2041 cgacatcctc cagctcgagc gcggcggcct tgtcgagcca ctgctgcgcg tctgccggct
     2101 gcgttttcgc ttgctcgacc accgaaagcg agccactcgc cggatcgcct tcgaaagcga
     2161 gcaggtattg accggagtcg gcctgccagt ggctggcgcc ctccctgacc accacgcgat
     2221 cggccactgc gcagatgcgc agccccgaca gcggcatggc atcaggcagg tgccggcgca
     2281 gctctttcat ggatcgcgcg attcgccggc gcggcacttt cgcctcgcac agctcctgcg
     2341 ccgtgcgcag cacgatcagg tcctggaagg agaactgcca ggcgccgcgc gggccccggt
     2401 tgggggccac gaatccggcc tggaccagcg cgcggaccgt gctgcgcggc aggtgcagca
     2461 gcttctccac ctcgcgcacg ccgtaggagt ccatggcgcg cggcgccgct atttcttcgc
     2521 agcctttctt tgcgccggct cagcctgggc gcgcttgggc ggcttgcgcg cttccggctg
     2581 cgcatccttc gctgccggcg ccgccttctt gccaaggctt gcgcgcagcg cctccataag
     2641 gtcgatgacc tgggcgccgg tctccggcgc ctccgccatg gtgatctcct ggccttccac
     2701 cttcttctgc accgcgacct cgatgcgcgc gcgcacctcg tcggtatagg cggccggatc
     2761 gaatttctcg gacgactgct gctcgatcag ctgttgcgcg agcttcagct cggcgggctt
     2821 gacctcggtc ttcggaatgt cgacgtcccg catcgagcga acttcgccgg cgtagagcag
     2881 ctgctgcatc accaggccct cgaccaggtc ctccaccggg cggatcatca cgatgtactg
     2941 cttgccgcgc gcggcccagc ggccgagcgc gcagcgaccg gtctcgcgca gcgcccgcgc
     3001 gagcagcgca tagggcttcg cgccgccctt gtcgggtccg aggtagtacg ccttgtcgaa
     3061 atacaccggg tccaccgttt tgagcggcac gaactcggtg atctcggcgc tgtgcgtgcc
     3121 cgcctcctcg agcgccttca gctcctcggg cgtgaacatc acgtactgat ccttggcgaa
     3181 ctcgtacccc ttggccatgt cttcgcgcgc aaccggcact tcctccttga cgcagaaata
     3241 ctgttgcttg aggcgggagc cgcaggcctt gtgcagcagg ttgaaggaga tcgcccggct
     3301 cgcctcggtg gccgaataaa gcttcaccgg tatggagacc attccgaacg agatagtcag
     3361 ggacgcaatc gat
//
LOCUS       DASYJA010000069         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1282915, whole genome shotgun sequence.
ACCESSION   DASYJA010000069 DASYJA010000000
VERSION     DASYJA010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282915"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1349)
                     /locus_tag="VGO02_01480"
     CDS             complement(57..1349)
                     /locus_tag="VGO02_01480"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7476387.1"
                     /translation="MTTPWLRGLRPYLEPAPLAALFLGVSSGFAFAMIGATLTTRLAQ
                     HGFSRGAVTAFTLTFLAYNLKFLWAPIIDQVRLPLIGRFGQRRSWLWLTGALVMVAVA
                     CLGIADPRESLAAFAFMAVAMAFAGATFDIIIDAYRIELLEPRQLGAGSGMSQYGWRI
                     GATAAGALALVLAQRLGWTGAYVACAVFALPAMVVGVFMGEPARHREPAKSHGVAQAL
                     VAYFSPLLEFLARQGALVVLLFVLIHKIGDTLANLTLRLLFQELGFTNDEVAFYDVGV
                     GFVALLLGILVGGILYSRLGMKRSVMWSLVLMAVSNLSFAVLAAAGHTNIGMAAAVGF
                     ENFASGIGGVTVVAYLSALCNLRFTATQYALLSAFASIVGRVVSGTAAGRMIDAMGYV
                     NYYLLTTLIALPGVFLFWYMIRSGLADLSIGSAGREEK"
     gene            complement(1330..2106)
                     /locus_tag="VGO02_01485"
     CDS             complement(1330..2106)
                     /locus_tag="VGO02_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEV7476388.1"
                     /translation="MRVITLNVNGLRSAASKGLFRWLAAQRADAVCLQEIKCQELDLD
                     AKLHGLKSFSSCYAFAEKKGYSGVALMSRRQPDEVRRGFASKEFDAEGRYVEARFGKL
                     WVISVYMPSGSAGPHRQASKFRFLRQFLVHLRKLQERGHQFILCGDWNIAHREIDLKN
                     WRSNQKNSGFLPEERAWLSQVFDELGYVDVFRRLNVKPEQYTWWSNRGQAWKKNVGWR
                     IDYQIATRGVAAKAVKESIYKTKRFSDHAPLTIDYDHTLA"
     gene            complement(2124..>2566)
                     /locus_tag="VGO02_01490"
     CDS             complement(2124..>2566)
                     /locus_tag="VGO02_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEV7476389.1"
                     /translation="AGIPYAAWIADRLGLPMQYVRKKPKGFGRNAQIEGDIRDGARTL
                     LVEDLTTDGRSKVNFCKALRDAGAIVDYVLVMFYYDIFPESKQIIADIKVKLLHLATW
                     WDVLRVVKQGGYFENRVIEEVERFLNAPAKWSAEHGGVSAFPES"
BASE COUNT          464 a          836 c          866 g          400 t
ORIGIN      
        1 gatgaggata tcggcagggc cgtcgacgag ccggcccccg cggatcagcg tgtggctcat
       61 ttctcctccc tgcccgcgga gccgatcgag aggtcggcga ggcccgagcg gatcatgtac
      121 cagaacagga acacgcccgg cagggcgatc agcgtggtga gcagatagta gttgacgtac
      181 cccatggcat cgatcatgcg gcccgccgcg gttccgctca ccacgcggcc gacgatgctg
      241 gcgaacgccg agagcagcgc gtactgcgtc gcggtgaacc gcaggttgca cagcgccgag
      301 aggtacgcga ccaccgtcac gccgccgatg ccgctcgcga agttctcgaa tcccacggcc
      361 gccgccatgc cgatgttggt atggccggcc gcggccagca cggcgaagct taaattcgat
      421 accgccatca ggacgaggct ccacatgacc gagcgcttca tgcccaggcg cgagtagagg
      481 atgccgccga ccaggatgcc gaggagcagg gccacgaatc ccacaccgac gtcatagaag
      541 gcgacctcgt cgttcgtgaa gccgagttcc tgaaagagca gcctcagggt gaggttagcc
      601 agcgtgtcgc cgatcttgtg aatgagcacg aacagcagga cgaccagggc gccctggcgc
      661 gccaggaact ccagcagcgg actgaagtag gcgacgagcg cctgcgcgac gccatgggac
      721 ttggcgggct cgcggtgccg ggccggctcg cccatgaaca cgccgaccac catggccggc
      781 agtgcgaaaa cggcgcaggc gacataggca ccggtccagc cgaggcgctg ggcgagcacc
      841 agcgcgagcg cgccggccgc ggtcgcgccg atgcgccagc cgtactggga catgccggat
      901 ccggcgccga gctggcgcgg ctcgagcagc tcgatgcgat aggcgtcgat gatgatgtcg
      961 aacgtcgcgc cggcgaacgc catggcgacc gccatgaacg cgaacgcggc gagagactct
     1021 cgcgggtcgg cgatgcccag gcaggccact gccaccatca ccagggcgcc ggtcagccac
     1081 agccagctgc gccgctggcc gaagcgtccg atgagcggca ggcgcacctg gtcgatgatc
     1141 ggcgcccaca ggaacttgag gttgtaggcg aggaacgtca gcgtaaacgc cgtgaccgcg
     1201 ccgcggctga acccgtgctg ggcaaggcgc gtggtcagcg tcgcaccgat catcgcaaag
     1261 gcgaaacccg aggagacgcc gaggaacagc gccgcgagcg gcgccggctc gaggtagggc
     1321 cgcaatcccc taagccaggg tgtggtcata gtcgatcgtc agcggcgcat ggtcggaaaa
     1381 gcgcttcgtc ttgtagatcg actccttgac cgccttcgcg gcgacgccgc gcgtggcgat
     1441 ctggtagtcg atgcgccagc ccacgttctt cttccaggcc tggccgcggt tcgaccacca
     1501 ggtgtactgc tcgggcttga cgttcagccg gcggaacacg tcgacgtagc caagctcgtc
     1561 gaagacctgc gagagccagg cgcgctcctc ggggaggaag cccgagttct tctggttgga
     1621 tcgccagttc ttgaggtcga tctcccggtg cgcgatgttc cagtcgccgc acaggatgaa
     1681 ttgatggccg cgctcttgga gcttcctcag gtgaaccaag aattgcctca aaaaacgaaa
     1741 cttcgaagcc tgccggtggg ggccggccga gcccgacggc atgtacaccg agatcaccca
     1801 gagttttccg aagcgcgcct cgacgtagcg gccttcggcg tcgaactcct tgcttgcgaa
     1861 gccgcgcctc acctcgtcgg gttgcctgcg gctcatcagc gctacgccgc tgtagccttt
     1921 cttctcggca aaggcgtagc aggaggagaa gcttttcagg ccgtgcagct tcgcgtcgag
     1981 gtccagctcc tggcacttga tctcctgcag gcagaccgcg tctgccctct gcgcggcgag
     2041 ccagcggaac agtcccttgc tggcggccga gcgcaggccg ttgacgttca gcgtgatgac
     2101 gcgcatggag gcgcggtgtt gcttcaggac tcggggaagg cggacacgcc gccgtgctcg
     2161 gccgaccact tggcgggagc attcaggaag cgctcgacct cctcgatcac gcggttctcg
     2221 aaatagccgc cctgcttcac caccctcagc acgtcccacc aggtggcgag gtgcaggagc
     2281 ttcaccttga tgtcggcgat gatctgcttc gactccggga agatgtcgta gtagaacatc
     2341 acgagcacgt agtccacgat cgccccggcg tcgcgcagcg ccttgcagaa gttgaccttg
     2401 ctgcgcccgt cggtggtgag gtcctcgacc agcagcgtgc gcgcgccgtc gcggatatcg
     2461 ccctcgatct gggcgttgcg gccgaagccc ttgggcttct tgcgcacgta ctgcatcggc
     2521 aggccgagac ggtcggcgat ccaggcggcg tacgggatac cggcgg
//
LOCUS       DASYJA010000070         4474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1282928, whole genome shotgun sequence.
ACCESSION   DASYJA010000070 DASYJA010000000
VERSION     DASYJA010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282928"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VGO02_01495"
     CDS             complement(<1..528)
                     /locus_tag="VGO02_01495"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEV7476390.1"
                     /translation="MRRFAVLCAALLAACATGNSDQKPPPLELPAAWKESAPRFTEDG
                     RWWRIYDDAQLDSTVEEALQNNADLVIAAARVDEARALVGEAQSAFFPTIDARGAASR
                     QQLSQRTAQAFPGIPTRFSDYRATLNVSYEVDIFGRLRAGARAARAELQASVAARETV
                     RLALAAQVAKSYFTLR"
     gene            complement(525..3728)
                     /locus_tag="VGO02_01500"
     CDS             complement(525..3728)
                     /locus_tag="VGO02_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEV7476391.1"
                     /translation="MFSRFFIDRPIFAAVLSIFVVIAGLASMRVLPVAQYPEIAPPVV
                     TVQAVYPGASAQVLEQTVAAPLENQINGVEHMIYMSSTSTSQGVVQIQVTFDIGTDVD
                     KAALNVNNRVKQAEPRLPLEVRRQGVTVEKGSSAFLQVLAFYSPDGRQDDVFISNYVT
                     MNVLDLVKRVPGTTNVQIFGAKDYAMRIWVRPDKLAQHRLTTADLIRALNEQNAQFAA
                     GKVGQSPSPKEQELVFTITTKGRLSDPKEFEQILLRSNPDGSALRLKDVARIELASKD
                     YDFIGRINGKTSTLMGVFLQPGANALAVAEDVKKTVGEISKRFPEGLAYSVPYDTTRF
                     VEVSIKEVVETLLIAMTLVFLVVYLFLQSWRATLIPFAAVPVSLIGTFAGLLMLGYSI
                     NTLTLFGMVLAIGIVVDDAIVVLENVERIMHEEGIPPRDAALKAMREVTSPIIAIVLV
                     LCAVFVPIMFLGGLAGELYRQFAVTISIAVVISGIVALTLTPALCVIVLRAEKRPQHG
                     FFGWFNRGFGRMTEHYESGVAWVIRRGAVGLALFAVMVAAAIFLWRITPGSLVPDEDQ
                     GFYIAAVVLPDGATLQRTDKVVAEVLQAIQSNPNNLDVVAFTGFDFLGGGFRNNAATI
                     FVTQKPWSERKVTTPMVVGDFFMKTGHIKEGLALAFGPPPIFGLGTAGGFEFYIQNRG
                     DGGPPQLAQALGAFLGAAHQDHRLGFVQTLWRPAVPQLSVDVDREKAKALGVPLEDVY
                     GTLAATLGSYYVNDFNKFGRTWQVLMSAEASYRDRPDDIGKVFVRSEKNEMIPLAALA
                     SVRYSAGPDALDRFNNLPAVKIFGQAAPGISSGQAIQTVEEIATKVLPSNISFDWGGA
                     SYQEKKSAGTSGLALALAAIMVFLILAAQYEKWSLPLSVLLALPFGTFGALAVIWVNN
                     LVFTPLLGRTPLTNDVYFQIGLVTLLGLAAKNAILIVEYAVMKHEQGLSASAAAVEAA
                     RLRFRPILMTSMAFILGVMPLVVSTGAGAGARHSAGTGVMGGMLAATFLAIFFVPMFF
                     KLLATRRLREQRSTAQIREEIERARAAPAAPERKEGQ"
     gene            complement(3731..>4474)
                     /locus_tag="VGO02_01505"
     CDS             complement(3731..>4474)
                     /locus_tag="VGO02_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV7476392.1"
                     /translation="EAIAAADLKAARARLVEARLNLGYTKVEAPAAGVASRSNRSEGS
                     LVSGPDVLLTTVMQVDPIWVNFGIPDNDAGRLQREVEAKRLVLPKGGFEVLLRLADGS
                     MYGKSGRLNFSDVRVNPSTGTREARAELPNPNGGALQPGQFVRVILKGATIPDAVAVP
                     QRAVLENPQGGKIVFTVDEKGAAQPRPVEVGEWAGENWIITKGLNQGDKVITDGVMKL
                     GPGAPVRIAEKKEAAEKKEAEQQKPPAKK"
BASE COUNT          720 a         1496 c         1540 g          718 t
ORIGIN      
        1 acgcagggtg aagtaggact tcgccacctg cgccgcgagc gcgaggcgca cggtctcgcg
       61 cgccgccacg ctcgcctgga gctcggcgcg cgcggcgcgg gcgcccgcgc gcagccggcc
      121 gaagatgtcc acttcatagg aaacgttgag ggtcgcgcga tagtcgctga acctcgtcgg
      181 aattcccgga aacgcctgcg ccgtgcgctg cgaaagctgc tggcgcgagg cggcgccgcg
      241 cgcgtcgatc gtcggaaaga aagccgattg cgcctcgccg accagcgccc gcgcttcgtc
      301 gacgcgggcc gcggcgatca cgaggtcggc gttgttctgc aacgcctctt ccaccgtgga
      361 atcgagctgt gcatcgtcgt agatgcgcca ccagcgcccg tcctccgtga agcgcggcgc
      421 gctttccttc cacgccgcag gcagctccaa cggtggcggc ttctggtcgg aatttccggt
      481 cgcgcaagcc gcgagcagcg cggcgcagag aacggcaaag cgcctcattg cccttccttc
      541 ctttcgggcg cggcgggcgc cgcgcgcgcg cgctcgatct cctcgcggat ctgggcggtg
      601 ctgcgctgct cgcgcaggcg ccgggtagcg agcagcttga agaacatcgg cacgaagaag
      661 atggcgagga aggtggcggc cagcatgccg cccatcacgc cggtgcccgc ggaatggcgc
      721 gcgccggcgc cggcgccggt cgacaccacc agcggcatca cgccgaggat gaacgccatc
      781 gaggtcatca ggatcgggcg gaagcgcagc cgcgccgcct ccaccgcggc ggcggacgcc
      841 gataggccct gctcgtgctt catcaccgcg tactcgacga tcaggatcgc gttcttcgcc
      901 gccaggccga gcagcgtgac caggccgatc tggaaataca cgtcgttggt cagcggcgtg
      961 cggccgagca gcggcgtgaa gaccaggttg ttgacccaga tcaccgccag cgcgccgaag
     1021 gtgccgaacg gcagcgcgag cagcaccgac agcggcagcg accacttctc gtactgcgcg
     1081 gcgaggatca ggaacaccat gatcgcggcg agcgcgagcg caagacccga ggtgccggcc
     1141 gatttcttct cctggtagga ggcaccgccc cagtcgaagc tgatgttgga gggcagcacc
     1201 ttggtggcga tctcctccac ggtctggatc gcctggcccg aggagatgcc gggggcggcc
     1261 tggccgaaga tcttcactgc cggcaggttg ttgaagcggt cgagcgcgtc gggcccggcg
     1321 ctgtaacgca cgctggccag cgcggcgagg gggatcattt cattcttttc gcttcttaca
     1381 aaaacctttc ctatgtcgtc gggccggtcg cgatacgaag cctcggccga catcagcacc
     1441 tgccaggtgc ggccgaactt gttgaagtcg ttgacgtagt agctgccgag cgtggcggcg
     1501 agcgtgccgt agacgtcctc tagcggcacg ccgagcgcct tcgccttctc ccggtcgacg
     1561 tcgaccgaga gctgcggcac cgccgggcgc cacagggtct gcacgaagcc gagccggtgg
     1621 tcctgatgcg ccgcgccgag gaaggcgccg agcgcctgcg ccagctgcgg cggaccgccg
     1681 tcgccgcggt tctggatata gaactcgaag ccgcctgcgg tgcccaggcc gaagatcggc
     1741 ggcgggccga aggcgagcgc gagcccttcc ttgatgtggc cggtcttcat gaagaaatcg
     1801 ccgaccacca tcggcgtggt gaccttgcgc tcgctccacg gcttctgcgt gacgaagatg
     1861 gtggcggcgt tgttgcggaa gccgccgccg aggaagtcga agccggtgaa ggccaccacg
     1921 tccaggttgt tggggttgga ctggatcgcc tgcagcacct cggccaccac cttgtcggtg
     1981 cgctgcaggg tcgcgccgtc gggcagcacc accgcggcga tgtagaaacc ctggtcttcg
     2041 tcgggcacca ggctgccggg cgtgatgcgc cagaggaaga tggcggcggc caccatcacg
     2101 gcgaacagcg cgaggccgac ggcgccgcgg cggatcaccc acgccacgcc cgattcgtag
     2161 tgctcggtca tgcgcccgaa gccgcggttg aaccagccga agaagccgtg ctgcggccgc
     2221 ttctccgccc gcaagacgat gacgcacagc gcgggagtga gcgtaagcgc cacgatcccc
     2281 gagatcacca cggcgatcga tatcgtcacc gcgaactggc ggtagagctc gccggccagg
     2341 ccgccgagga acatgatcgg cacgaacacc gcgcacagca ccaggacgat ggcgatgatc
     2401 gggctggtga cctcgcgcat cgccttgagc gcggcgtcgc gcggcggaat gccctcctcg
     2461 tgcatgatcc gctcgacgtt ctccaagacc acgatggcgt cgtcgacgac gatgccgatg
     2521 gccagcacca tgccgaagag cgtcagcgtg ttgatcgagt agccgagcat cagcaggccg
     2581 gcgaaggtgc cgatcagcga aaccgggacc gcggcgaagg ggatgagcgt cgcgcgccag
     2641 ctctgcagga acaggtacac caccaggaac accagcgtca tcgcgatgag cagcgtctca
     2701 accacctcct tgatcgacac ctcgacgaag cgcgtggtgt cgtagggcac cgagtaggcg
     2761 agcccctccg ggaagcgctt ggagatctcg ccgacggtct tcttgacgtc ttcggccacc
     2821 gcgagcgcgt tggcgccggg ctgcaggaac acgcccatca gcgtcgaggt cttgccgttg
     2881 atgcggccga tgaagtcgta gtccttggag gcgagctcga tccgcgccac atccttcagg
     2941 cgcagcgcgg agccgtcggg gttggagcgc agcaggatct gctcgaactc cttcggatcg
     3001 gacaggcgcc ccttggtagt gatggtgaag accagctcct gctccttcgg cgagggcgac
     3061 tgccccacct tgccggcggc gaactgcgcg ttctgctcgt tcagcgcgcg gatcaggtcg
     3121 gcggtggtca ggcggtgctg cgcgagcttg tccgggcgca cccagatgcg catcgcgtag
     3181 tccttggcgc cgaagatctg cacgttggtg gtgccgggga cgcgcttcac caggtcgagc
     3241 acgttcatcg tcacgtagtt cgagatgaag acgtcgtcct ggcggccgtc gggcgagtag
     3301 aaggcgagca cctgcaggaa ggcggaggag cccttctcca ccgtcacgcc ctggcggcgc
     3361 acctccagcg gcagcctggg ctcggcctgc ttgacgcggt tgttgacgtt gagcgccgcc
     3421 ttgtcgacgt cggtgccgat gtcgaaggtg acctggatct gcaccacgcc ctgcgaggtc
     3481 gaggtcgagc tcatgtagat catgtgctcg acgccgttga tctggttctc gagcggcgcg
     3541 gcgacggtct gctccagcac ctgcgccgag gcgcccgggt agaccgcctg cacggtgacc
     3601 accggcggcg cgatctcggg gtactgcgcg accggcagca cgcgcatcga ggcgaggccg
     3661 gcgatgacca cgaaaatcga cagaacggcg gcgaagatcg gacgatcgat gaagaatctg
     3721 gagaacatga ttacttcttg gccggcggtt tttgctgttc ggcttctttt ttctctgctg
     3781 cttctttttt ctctgcaatg cgaaccggtg cgccgggacc gagcttcatc acgccgtcgg
     3841 tgatcacctt gtcgccctgg ttgagcccct tggtgatgat ccagttctcg cccgcccact
     3901 cgccgacctc caccgggcgc ggctgcgcgg ctcccttctc gtccaccgtg aacacgatct
     3961 tgccgccctg cgggttctcg agcaccgcgc gttgcggcac cgcgaccgcg tcggggatcg
     4021 tcgcgccctt caggatcacg cgcacgaact ggcccggctg cagcgcgccg ccgttgggat
     4081 tgggcagctc ggcgcgcgcc tcgcgcgtgc cggtgctcgg attgacgcgc acgtcggaga
     4141 aattcaggcg gccggacttg ccgtacatcg agccgtcggc caggcgcagg agcacctcga
     4201 agccgccttt cggcagcacc aggcgcttgg cttcgacctc gcgctgcagg cggcccgcat
     4261 cgttgtcggg gatgccgaag ttgacccaga tcgggtcgac ctgcatcacc gtcgtcagca
     4321 gcacgtcggg gcccgagacg agcgagccct cggagcggtt cgagcggctc gccacgccgg
     4381 ccgcgggcgc ttcgaccttg gtgtagccga ggttgagcct cgcctccacc agccgcgcgc
     4441 gcgccgcctt caggtcagcg gcggcgatcg cctc
//
LOCUS       DASYJA010000071         4196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_11801, whole genome shotgun sequence.
ACCESSION   DASYJA010000071 DASYJA010000000
VERSION     DASYJA010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11801"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VGO02_01510"
     CDS             <1..224
                     /locus_tag="VGO02_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HEV7476393.1"
                     /translation="LRAEDTSWTSGRSPEDVTHTAKTRYRQADAACTVTATDTGIKVD
                     FAAPQWAVTPGQSVVLYDGEACLGGGVIT"
     gene            complement(217..828)
                     /locus_tag="VGO02_01515"
     CDS             complement(217..828)
                     /locus_tag="VGO02_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEV7476394.1"
                     /translation="MKLLAALISPYTRKVRVVLAEKKIDCEMQIVDVNPADNPVNPHN
                     PLGKIPTLLLDDGTALYDSRVIVEFLDSASPISRLIPEATRDRVAVRRWEALADGVLD
                     AGLLVRYESLRDKKEQSKAWTDKQVARMKRGMDQIAKEIGAHCHADRYSLADIAVACC
                     TGWLDFRKPGGVDWRGEYAPVARHYDKMMERPAIADTVPQQPK"
     gene            complement(841..1401)
                     /locus_tag="VGO02_01520"
     CDS             complement(841..1401)
                     /locus_tag="VGO02_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEV7476395.1"
                     /translation="MRVLLSEAKACRLCEKHLEPRPVFRVGAKARLLIVGQAPGRRVH
                     ETGLPWNDPSGDVLREWLGLEREAFYDTSRIAIVPIGLCYPGTVDGADLPPRRECAPL
                     WQPRFRAALPNVELTLLVGSYAQNFYLSATKKSSLAQTVRAYRDYLPEFFPLPHPSWR
                     NIGWLKSNPWFAKDVLPALRRRVRLV"
     gene            1420..2784
                     /gene="purB"
                     /locus_tag="VGO02_01525"
     CDS             1420..2784
                     /gene="purB"
                     /locus_tag="VGO02_01525"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEV7476396.1"
                     /translation="MPSPLAALSPLDGRYARTADPLRAFFSEQALIGYRVKVELAWLE
                     ALATERSIKEIKPFSSAARKAFASLEKNFSERDAEHIKNIEAETNHDVKAIEYWLRSK
                     LAKNAEASKALEFIHFACTSEDINNLSYALMMAESRERVMLPRLDELVEVLRALAKRH
                     AGAAMLSRTHGQPASPTTLGKEMANFAHRLERARGRIARVAVLGKANGVVGNYNAHVI
                     AYPGVDWQRFGKRFVERLGLEANAYTTQIDPHDWLAELLDAYAHANSVLLDLDRDLWG
                     YISLGYFRQRPKKGEVGSSTMPHKVNPIDFENSEGNVGIANALLRHLADKLPVSRWQR
                     DLSDSTAMRNIGAALGHTLLAYASCLRGLGKLEVDPRRLAADLDANWEVLSEAVQQVM
                     RRHGVPDAYDKLKAVSHGKRLDRRQLAAFVKSLPIPAEAKKRLLALTPGKYTGLAEAL
                     AKKV"
     gene            2781..3557
                     /locus_tag="VGO02_01530"
     CDS             2781..3557
                     /locus_tag="VGO02_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7476397.1"
                     /translation="MRFCVVVPLLFCLTTEAQEIVTVATRPGVTQSFFVAGMGERKAE
                     AVALLYVGGGGIIRLRMEGGQPKFGANNFLPRSRGEFIRDGVLPVVMDAPSDQPGGLT
                     DYYRSSGQQAADARGVLAELKKRFPQLPVFLVGTSRGTISAAFLGRALGNEVAGVVLT
                     STLFVSPRGASLAGFDYASIKAPLLFVHHREDGCEQTPYTEAHRLAARYPLVSVKGGK
                     PPESGPCDPFAPHGFWGKEPQTVDAIAGWMLNKPYPREID"
     gene            3564..>4196
                     /locus_tag="VGO02_01535"
     CDS             3564..>4196
                     /locus_tag="VGO02_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476398.1"
                     /translation="MLHAATIFLSSFLLFLVQPLIARLILPWFGGSAAVWTTCMLFFQ
                     TVLLAGYAYAHFLHARVPGRAQAIVHTLLLAAAVALLPISPGEGWKPAGGEEPISRIL
                     LLLCASVGLPYLILASTSPLLQAWYVRAHPQGNPYRLFAISNLASLGALVGYPFLVEP
                     IFTAREQVIGWSLLFAGFAVLCSAVAWLTPPAATREESSVSENSKAPYLLW"
BASE COUNT          661 a         1461 c         1385 g          689 t
ORIGIN      
        1 ccctccgggc ggaagacacg agctggacct ccggacgctc gcctgaggac gtcactcata
       61 ccgcaaaaac ccggtaccgc caggcggatg cggcttgcac cgtcacggca accgataccg
      121 gcatcaaagt cgatttcgcc gccccgcaat gggcggtcac gccgggccag tcggtggtgc
      181 tctacgacgg cgaagcctgc ctgggcggcg gggtgattac ttaggctgct gcggaacggt
      241 gtcggcgatc gccggccgct ccatcatctt gtcgtagtgg cgggcgacgg gggcgtactc
      301 cccgcgccaa tcgacgccgc cgggcttcct gaagtcgagc cagcccgtgc agcaggccac
      361 cgcgatgtcg gcgagcgaat agcggtcggc atggcagtgg gcgccgatct ccttcgcgat
      421 ctggtccatg ccgcgcttca tgcgcgcgac ctgcttgtcg gtccaggcct tgctctgctc
      481 cttcttgtcc ctcagggatt cgtagcgcac cagcagcccg gcgtcgagca cgccgtcggc
      541 gagcgcttcc cagcggcgca ccgcgacgcg gtcgcgcgtg gcctcgggga tgaggcggct
      601 gatcggcgag gcgctgtcga ggaactcgac gatgacgcgc gagtcgtaga gcgcggtgcc
      661 gtcgtcgagc agcagcgtcg ggatcttgcc gagcggattg tgcgggttga ccggattgtc
      721 ggccggattg acgtcgacga tctgcatctc gcagtcgatc ttcttctcgg cgagcaccac
      781 gcgcaccttg cgcgtgtacg ggctgatcag tgcggcgagt agcttcatga gggcgccgga
      841 ttatacaagc cgcacgcgcc ggcgcagcgc cggcagaaca tcttttgcaa accatggatt
      901 gctcttcagc cagccgatgt tgcgccagct cgggtgcggc agcgggaaga actcgggcag
      961 gtagtcgcgg tacgcgcgga cggtctgcgc aagcgagctt tttttggtgg cggaaagata
     1021 gaagttctga gcataacttc cgactagaag cgtgagctct acgttcggca aggccgcgcg
     1081 aaagcgcggc tgccacagcg gcgcgcattc gcgccgcggc ggcagatcgg cgccgtccac
     1141 cgtgcccggg tagcacaggc cgatcggcac gatggcgatg cgcgaagtgt cgtagaacgc
     1201 ctcgcgctcg aggccgagcc attcgcgcag cacgtcgccc gagggatcgt tccagggcag
     1261 gccggtctcg tgcacgcggc gccccggcgc ctggccgacg atcagcagcc tggccttcgc
     1321 gccgacgcgg aataccggcc gcggctcgag gtgcttctcg catagccggc aggccttcgc
     1381 ctcggagagc aaaacgcgca tcccctctat aatagggcca tgccctcgcc gctcgccgcg
     1441 ctctcgccgc tcgacgggcg atacgccagg accgccgacc cgctgcgcgc gttcttcagc
     1501 gagcaggccc tcatcggcta ccgcgtcaag gtcgagctcg cctggctgga ggccctggcc
     1561 acggagcgct ccatcaagga aatcaagcct ttttcgtccg ccgcgagaaa ggcctttgct
     1621 tccctggaga aaaacttttc cgagcgcgat gcggagcaca tcaagaacat cgaggccgag
     1681 accaaccacg acgtcaaggc gatcgagtac tggctgcgct cgaagctggc gaagaacgcc
     1741 gaggcgagca aggcgctgga attcatccac ttcgcctgca cctcggaaga catcaacaac
     1801 ctgtcgtacg cgctgatgat ggccgagtcg cgcgagcggg tgatgctgcc gcgcctggac
     1861 gagctggtcg aggtgctgcg cgcactggcg aagcgccatg ccggcgccgc gatgctctcg
     1921 cgcacccatg gccagccggc ctcgccgacc acgctcggca aggagatggc gaacttcgcg
     1981 caccgcctgg agcgcgcgcg cgggcgcatc gccagggtcg cggtgctcgg caaggcgaac
     2041 ggcgtggtcg gcaactacaa cgcgcacgtc atcgcctatc ccggcgtcga ctggcagcgc
     2101 ttcggcaagc gcttcgtcga gcgcctgggc ctcgaggcca acgcctacac cacgcagatc
     2161 gatccgcacg actggctggc ggagctgctg gacgcctatg cgcacgccaa cagcgtgctg
     2221 ctcgacctcg accgcgacct gtggggctac atctcgctcg gctacttccg ccagcggccg
     2281 aagaagggcg aggtcggctc ctcgaccatg ccgcacaagg tcaacccgat cgacttcgag
     2341 aattccgagg gcaacgtcgg catcgccaat gccctgctgc gccacctcgc cgacaagctg
     2401 ccggtgtcgc gctggcagcg cgacctgtcg gactccaccg caatgcgcaa catcggcgcc
     2461 gcgctcggcc acaccctgct cgcctacgcc tcgtgcctgc gcggcctggg caagctcgag
     2521 gtcgacccgc ggcgcctcgc cgccgacctg gacgcgaact gggaagtgct cagcgaggcg
     2581 gtgcagcagg tgatgcgccg ccacggcgtg cccgacgcct acgacaagct caaggccgta
     2641 agccacggca agcgtctcga ccgccgccag ctcgccgcgt tcgtcaaatc gctgccgatc
     2701 ccggccgagg ccaagaaacg cctgctcgcg ctcacccccg ggaaatacac cggtctggcg
     2761 gaggcgctgg cgaagaaggt atgaggtttt gcgttgtcgt accgcttctc ttctgtctga
     2821 caactgaagc acaggagatc gtcaccgtcg cgacccggcc cggcgtcacg cagtccttct
     2881 tcgtcgccgg catgggcgag cgcaaggccg aagcggtggc gctgctctac gtcggcggcg
     2941 gcggcatcat ccgcctgcgg atggagggcg gacagccgaa gttcggcgcc aacaacttcc
     3001 tgccgcgctc gcgcggcgag ttcatccgcg acggcgtgct gccggtggtg atggacgcgc
     3061 cttccgacca gccgggcggc ctgaccgact actaccgctc gagcggccag caggccgccg
     3121 acgcgcgcgg ggtgctcgcc gagctgaaga agcgcttccc gcagctcccg gtgttcctgg
     3181 tcggcacgag ccgcggcacg atcagcgccg cgttcctcgg ccgcgccctg ggcaacgagg
     3241 tcgccggcgt ggtgctcacc tcgaccctgt tcgtgtcgcc gcgcggggcg agcctcgcgg
     3301 gcttcgacta cgcctcgatc aaggcgccgc tgctcttcgt ccaccatcgg gaggacggct
     3361 gcgagcagac gccgtatacc gaggcgcacc gccttgcggc gcgctatccg ctggtatcgg
     3421 tgaagggcgg caagccgccg gagtcggggc cgtgcgaccc gttcgcgccg cacggcttct
     3481 ggggcaagga gccgcaaacc gtcgacgcca tcgccggctg gatgctgaac aagccctacc
     3541 cgcgcgagat cgactgacgc gcgatgctgc acgcagccac catattcctg tcgtcgttcc
     3601 tgctgttcct cgtgcagccg ctgatcgcgc gcctgatcct gccgtggttc ggcggcagcg
     3661 cggcggtgtg gaccacctgc atgctgttct tccagacggt cctgctcgcc ggctacgcct
     3721 acgcgcattt cctccacgcc agggtccccg gcagggcgca ggcgattgtg catacgctcc
     3781 tgcttgcagc ggcagttgcg ctcctgccga tctcgccggg cgaggggtgg aagccggccg
     3841 gcggggagga gccgatctcg cgcatcctcc tgctgctgtg cgcatccgtg ggactgccgt
     3901 acctgatcct cgcctcgacc agcccgctgc tccaggcgtg gtacgtgcgc gcgcacccgc
     3961 agggcaatcc ctaccgcctg ttcgccatct cgaacctcgc ttccctcggc gcgctcgtcg
     4021 gctacccgtt cctggtcgag ccgatcttca ccgcccgcga gcaggtcatc ggctggtcgc
     4081 ttcttttcgc aggattcgcg gttctctgtt cggctgtcgc ctggcttact cctccggccg
     4141 ctacaaggga agaatcttca gtttcagaaa attcaaaagc gccttacctg ctatgg
//
LOCUS       DASYJA010000072         2818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1101457, whole genome shotgun sequence.
ACCESSION   DASYJA010000072 DASYJA010000000
VERSION     DASYJA010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101457"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VGO02_01540"
     CDS             complement(<1..483)
                     /locus_tag="VGO02_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEV7476399.1"
                     /translation="MLKRGSSLLAVTLVVVLAGCGKSGPDSSGTANAATSPVAKELKL
                     TEASRKFLVIEPVGGVEPAGGRTYFGRVAFRPKALSAVTAPFAGRIASVLVEPGQRVS
                     AGTVLFTIDSADVLSVRAALDQARIKLRTAEEVFARQSEMVKRGVGIEAERFDAEMKL
                     R"
     gene            505..1197
                     /locus_tag="VGO02_01545"
     CDS             505..1197
                     /locus_tag="VGO02_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476400.1"
                     /translation="MKIARISNKGRQRLTLAFHLTAFFLSFTGHPAHAARPFNTDDAR
                     VVDPGGCQVESFYKKQETYSGSEFWFMPACNPGSFELTAGANRIEGHDSVILQAKVLM
                     KTLQTNGVGFAGSMGVFGGDPYVNGIMSVSFLDDRAVVHLNLGMIQDNEANITRGTWG
                     VGLEALLFAPRIYGIFESYAQKGDKPTLHYGVRFWVVPNRFQIDATHGQQNASDTAPE
                     RRFNSVGLRILW"
     gene            complement(1166..2458)
                     /locus_tag="VGO02_01550"
     CDS             complement(1166..2458)
                     /locus_tag="VGO02_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEV7476401.1"
                     /translation="MLSKEQNELLCRVEGAAPMGQMMRRHWLPLCLSEEVAERDGAPV
                     RARLLGEDLVVFKDTNGNIGVLDERCPHRGASLVFGRNEECGLRCLYHGWKFDVDGKC
                     LDMSSEPGGGASVRGAMRTKAYPAREGGGFVWIWMGPAQHLSEFEPPAWAPHPHCRTA
                     IVKMQAECNWAQVLEGSIDSAHSSSLHSTEMPAAQVDSAKATGKVWPRPSDDKAPKLQ
                     FEPTSYGFRYAALRRPVQNAESHQYVRTTLFIAPFTVLIPPNDQYNLAQMLVPIDDVN
                     TMFYWVAWHPDQTKGISQEEWRAFCAATVGVDLDSDFRKKRTLKNHYLQDRAAMKQGD
                     FTGIKGIPTQDMAMWESMGPIADRSRDHPGSSDVAVVQFRRMMVAAAKKFQEGAPAIG
                     TAEPRVAHVELASFEGILPKSADWRAAGATRGSAVPQS"
     gene            2542..>2818
                     /locus_tag="VGO02_01555"
     CDS             2542..>2818
                     /locus_tag="VGO02_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV7476402.1"
                     /translation="MLPALVRDPRVALVGAADPRAEARAQFEADFQCKVYREVADLCA
                     DPNVEAVYVATPHQFHAAHAVAALAAGKHVLVEKPMALSLDECRAMIA"
BASE COUNT          467 a          961 c          907 g          483 t
ORIGIN      
        1 gcgcagcttc atctcggcgt cgaagcgctc ggcctcgatg ccgacgccgc gcttcaccat
       61 ctcgctctgg cgcgcgaaga cttcctcggc cgtgcgcagc ttgatgcgcg cctggtcgag
      121 cgcggcgcgc accgacagca cgtcggcgct gtcgatggtg aacaggaccg tgccggcgct
      181 cacgcgctgc ccgggctcga ccagcaccga ggcaatgcgg ccggcgaacg gcgcggtcac
      241 cgcggacagc gccttcgggc ggaacgcaac gcggccgaaa taggtgcgac cgcccgccgg
      301 ctcgacgccg ccgaccggct cgatcaccag gaacttgcgc gatgcttcgg tgagtttcaa
      361 ctccttcgcg accgggctgg tcgcggcgtt tgcggtgccg ctagaatccg ggccggactt
      421 gccgcaaccg gcgagcacaa caacaagcgt cacagccagc agggacgatc cccgcttgag
      481 catccgaatc cccctaggac tcaaatgaag atcgcgagga tatccaacaa agggcggcag
      541 aggctgacgc tagcctttca tttaacagcg ttttttcttt cttttacagg ccacccggcg
      601 catgcggcgc ggcccttcaa caccgacgac gcgcgcgtgg tggacccggg cggctgccag
      661 gtggagagct tctacaagaa gcaggagacc tactccggct cggagttctg gttcatgccg
      721 gcgtgcaacc cgggcagctt cgagctcacc gccggcgcca accgcatcga gggccatgat
      781 tcagtcatcc ttcaggccaa ggtgctgatg aagacgttgc agaccaatgg cgtaggcttc
      841 gccggctcga tgggcgtgtt cggcggcgat ccgtacgtga acggcatcat gagcgtctcg
      901 ttcctcgacg accgcgccgt ggtgcacctc aacctcggca tgatccagga caacgaagcg
      961 aacatcacgc gcggcacctg gggcgtcggc ctggaggcgc tgctattcgc gccgcggatc
     1021 tacggcattt tcgaaagcta cgcgcagaaa ggcgacaagc cgacgctgca ctacggcgtg
     1081 cgcttctggg tcgtgccgaa ccggttccag atcgacgcca cccacgggca gcagaacgcc
     1141 tcggacacgg cgcccgagcg gcgcttcaac tctgtgggac tgcggatcct ctggtagcgc
     1201 cggcggcgcg ccagtcggcg ctcttcggca ggatgccctc gaacgaggcg agctcgacgt
     1261 gcgcgacgcg cggctcggcg gtgccgatgg ccggcgcgcc ctcctggaac ttcttcgccg
     1321 ccgccaccat catgcggcgg aactgcacca ccgcgacgtc gctcgagccc ggatggtccc
     1381 ggctgcggtc ggcgatcggc cccatcgact cccacatcgc catgtcctgc gtcgggatgc
     1441 ccttgatgcc ggtgaagtct ccctgcttca tcgctgcgcg gtcctgcagg taatggtttt
     1501 tcaaagttct tttttttctg aagtcggaat cgagatcgac tcccaccgtt gcagcacaga
     1561 aggcccgcca ttcctcctgc gagataccct tcgtctgatc gggatgccag gccacccagt
     1621 agaacatcgt gttgacgtcg tcgatcggca ccagcatctg cgccaggttg tactggtcgt
     1681 tgggcgggat cagcacggta aaaggcgcga tgaacagagt ggttctgacg tattgatggg
     1741 actctgcgtt ctggacaggc cggcgcagcg ccgcgtagcg gaacccatac gacgtcggct
     1801 caaactggag cttcggcgcc ttgtcgtcgg acggccgcgg ccagaccttg ccggtggcct
     1861 tggcgctgtc gacctgcgcc gccggcatct cggtggagtg caggctcgag ctgtgcgccg
     1921 agtcgatcga gccctccagc acctgcgccc agttgcactc ggcctgcatc ttgacgatcg
     1981 cggtgcggca gtgcgggtgc ggcgcccagg ccggcggctc gaactcgctc aggtgctgcg
     2041 cagggcccat ccagatccag acgaagccgc cgccttcgcg cgccgggtag gctttggtgc
     2101 gcatcgcgcc gcggaccgac gcgccgccgc cgggctcgga agacatgtcg aggcacttgc
     2161 cgtctacgtc gaacttccag ccgtggtaga ggcagcgcaa cccgcactcc tcgttgcgcc
     2221 cgaacaccag cgacgcgccg cggtgcgggc aacgttcgtc gagaacgccg atgttgccgt
     2281 ttgtatcctt gaagactact aaatcttccc caagcaagcg ggcgcggact ggcgcgccgt
     2341 cgcgctcggc gacctcttcc gacaggcaca gcggcagcca gtggcggcgc atcatctgcc
     2401 ccatcggggc ggcgccttcc acccggcaca gcagctcgtt ctgctctttc gacaacatcg
     2461 gtatacttag cctcctaagt atccacgatg ttaccaaaac tcaaaatcgg tgtcgcgggt
     2521 ctcgggcgcg cattcgcagt catgctccct gccctggtgc gcgacccgcg ggtggcgctg
     2581 gtgggcgccg cggacccgcg ggccgaggcg cgcgcgcagt tcgaggccga cttccagtgc
     2641 aaggtctacc gcgaggtcgc cgacctgtgc gccgacccga acgtcgaagc ggtctatgtc
     2701 gcgacgccgc atcagttcca tgccgcgcac gccgtggccg cgctcgccgc cggcaagcac
     2761 gtcctggtgg aaaagccgat ggccctctcg ctcgacgaat gccgggcgat gatcgctg
//
LOCUS       DASYJA010000073         4730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1010864, whole genome shotgun sequence.
ACCESSION   DASYJA010000073 DASYJA010000000
VERSION     DASYJA010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010864"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..975)
                     /locus_tag="VGO02_01560"
     CDS             complement(139..975)
                     /locus_tag="VGO02_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV7476403.1"
                     /translation="MNKPKGLGRGLDALLGRDESPREALLNLPVGRIRPGKYQPRTKM
                     DQQALAELAGSIRSQGLMQPLLVRPVDRDRYELIAGERRWRAAQMAGLEEVPVLVREV
                     PDDAALAMSLIENIQRENLNPMEEAAGVQRLVDEFKMTHEQAADSVGRSRSATTNLLR
                     LLKLARPVQEMLMEGALEMGHARALLSLDPARQIEAGNRVAAKGLSVRETEALVQTWL
                     RGPTPRRKKRGDRDLARLEEEASERLGTTVEIRAGRKGAGKVVVHYSGLDHLDQLLKK
                     LR"
     gene            complement(972..1742)
                     /locus_tag="VGO02_01565"
     CDS             complement(972..1742)
                     /locus_tag="VGO02_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7476404.1"
                     /translation="MARVLAVVNQKGGVGKTTTSVNLAAALAQAGRRVLLVDLDPQGN
                     ATMGSGIDKRVVGRTVYHVLLGLGDLTTIRTRAERGGYDLVPANRELAGAEIELVELP
                     NRETRLKDALERVAQDYDFILIDCPPSLSLLTVNALTAAQRVLIPMQCEYYALEGLSD
                     LVGTIKRVRSNLNPAIEIAGLLRTMYDPRNMLSQQVSQQLEAHFGDKVYRTLVPRNVR
                     LAEAPSYGVPAVVWDAASKGAQAYIALADEILAQGQPA"
     gene            complement(1743..2357)
                     /gene="rsmG"
                     /locus_tag="VGO02_01570"
     CDS             complement(1743..2357)
                     /gene="rsmG"
                     /locus_tag="VGO02_01570"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011402670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEV7476405.1"
                     /translation="MTPRAALERGLDELAVALPAGVSEQLLAYVDLLMKWNRTYNLTA
                     IRDPLEMVSRHLLDSLAVLTHLPMTDGAALADVGSGAGLPGIPLAIARGAWRVTVNDS
                     RQKKAAFLRQAAIELGLRNVEVHEGRVESWRPAARFAVVISRAFADLGEFISKCRHLV
                     ARGGCLAAMKGTAPGAIDARCSVVALRVPLLDAQRHVVLCRLED"
     gene            complement(2354..4186)
                     /gene="mnmG"
                     /locus_tag="VGO02_01575"
     CDS             complement(2354..4186)
                     /gene="mnmG"
                     /locus_tag="VGO02_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEV7476406.1"
                     /translation="MLFPTEFDVIVIGGGHAGTEAALAAARMGCATLLLTHSIETLGQ
                     MSCNPSIGGIGKGHLVKEIDALGGAMALATDEAGIQFRILNSSKGAAVRATRAQADRV
                     LYRGAIRRRLENQPNLAIFQQAVDDISVEGDHAVGVVTQTGLRFRSRTIVLTTGTFLS
                     GLIHVGLQSYQAGRAGDPAAVTLGARLREMKLPVGRLKTGTPPRLDARSIDFSAMERQ
                     PGDRPEPVFSFLGRRDMHPEQLPCWITHTNAGTHDIIRAGLDRSPMFTGVIQGVGPRY
                     CPSIEDKIHRFAGKASHQIFLEPEGLTTNEIYPNGISTSLPLDVQLDLVHSIRGLEKA
                     HIVRPGYAIEYDYFDPRNLRPSLETKSIHGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RKVRGMDPWWPKRDQGYLGVLVDDLTTSGVSEPYRMFTSRAEYRLTLREDNADLRLTE
                     IGRELGLVDDARWEAFSRKRDAIDGETERLKSTYVSAGLQQKSSIYELLRRPEVTYRG
                     IVETPVSDPAVAEQVEIQAKYEGYISRQRAEVERRKGAESTRLPNDLDYRGVRGLSAE
                     VQQKLNRHRPETIGQASRISGVTPAAISLLLVHLKRGFPQKKTA"
     gene            complement(4312..>4730)
                     /locus_tag="VGO02_01580"
     CDS             complement(4312..>4730)
                     /locus_tag="VGO02_01580"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009911895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HEV7476407.1"
                     /translation="FCGYNMADYFAHWLKMGRTVQQPPRIFRVNWFRRDANGKFMWPG
                     FGENMRVLQWVVERCQGHGHGVETPLGTMPRYEDLTWTGLDKLSPAQFAELERVDNGA
                     WREELSAHDELFGKFGARLPEALEMRRGRMHQKLAA"
BASE COUNT          785 a         1579 c         1527 g          839 t
ORIGIN      
        1 aaacggtgcg gatcggcctg cgcagcatgc caaaacccgc gttcatcctc cccgccccac
       61 gcaaagccgc gcgattttag cgtttcgcga caaggccggt caacgcgttg cgccgcaaca
      121 tcgactcggg ttcgctgctc accgtagctt cttcagcaac tggtcgaggt gatcgaggcc
      181 cgagtagtgc accaccacct taccggcccc cttgcgtccg gcgcggatct cgacagtggt
      241 gcccagcctc tccgaggcct cctcttccag ccgcgccagg tcgcggtcgc cgcgcttctt
      301 gcgtcgcggc gtgggaccgc gcagccaggt ctgcaccagc gcttcggtct cgcgcaccga
      361 caggcctttt gccgcaacgc ggttgccagc ctcgatctgc cgcgcggggt cgagggagag
      421 cagcgcgcgc gcgtgcccca tctccagggc gccctccatc agcatctcct ggaccggcct
      481 tgccagcttg agcagccgca gcaggttggt ggtggcggag cgcgaacgtc caaccgaatc
      541 ggccgcctgc tcgtgcgtca tcttgaattc gtcgaccagg cgctgcacgc cagcggcttc
      601 ctccatcggg ttgaggttct cgcgctggat gttctcgatc aaggacatcg ccaacgccgc
      661 gtcgtcaggc acctcgcgca ccagcacggg aacctcctcg agtccggcca tctgcgcggc
      721 gcgccagcgc cgctcgcccg cgatcagctc gtagcggtcg cgatccaccg gtcgcacgag
      781 aagcggctgc atgaggcctt gcgagcggat cgagccggcc agctcggcga gcgcctgctg
      841 gtccatcttc gtgcgcggct gatacttgcc gggccggatc cgcccgacgg gtaggttgag
      901 gagcgcctcg cgcggcgact cgtcgcggcc gagcagggcg tccaggccgc ggcccagtcc
      961 cttgggtttg ttcatgcggg ttgtccctga gcgaggattt catcggcgag cgcgatataa
     1021 gcctgcgccc ccttggaagc cgcgtcccat accactgccg gaacgccata gctcggcgcc
     1081 tcggcaagcc gtacgttgcg cggcacgagc gtgcggtaga ccttgtcgcc gaagtgcgct
     1141 tcgagctgct gcgaaacctg ctgcgacagc atgttgcggg ggtcgtacat cgtgcgcagt
     1201 aacccggcga tctcgatcgc aggattcagg ttggagcgca cccgcttgat ggtgccgacc
     1261 aggtcggaga ggccctcgag cgcgtagtac tcgcactgca tcggaatcag cacgcgttgc
     1321 gcggccgtca gtgcattgac cgtgagcagg ctcagcgagg gcgggcaatc gatcaggatg
     1381 aagtcgtagt cctgcgccac gcgctcgagc gcgtccttca gccgggtctc gcggttcggc
     1441 agctcgacca gctcgatttc cgcgccggcg agctcgcggt ttgccggcac gaggtcgtag
     1501 ccgccgcgct cggcccgtgt gcggatggtc gtgagatcgc cgaggccgag caggacgtgg
     1561 tagacggtgc gtccgacaac gcgcttgtcg atgccgcttc ccatggtggc gttgccctgg
     1621 gggtcgaggt ccaccagcag gacgcgccgg ccggcctgcg cgagcgcagc ggcgaggttg
     1681 acgctcgtcg tggtcttgcc gacgccgcct ttctggttga ccaccgcgag gacgcgcgcc
     1741 actcagtcct ccagccggca aagcacgacg tggcgttgcg catcgagcaa tggaaccctg
     1801 agcgcaacga cgctgcaacg cgcgtcgatc gcgcccggag cagttccctt catggcggcg
     1861 aggcagccgc cgcgagcgac cagatggcga cacttcgaga tgaattcgcc gaggtcggca
     1921 aaggcgcgcg agatgacgac cgcaaagcgc gccgcaggac gccagctctc gacgcgtccc
     1981 tcgtgcacct cgacgttgcg caggcccagt tcgatcgccg cctggcgcag gaacgccgcc
     2041 ttcttctgcc tggagtcgtt caccgtcacg cgccatgcgc cgcgcgcgat cgccagcggg
     2101 atacccggca gacctgcgcc cgagccgaca tcagccagcg cggcgccgtc cgtcatcggc
     2161 aaatgcgtca gcactgcgag tgaatcgagc agatggcggc tcaccatttc cagcggatcg
     2221 cgaatcgcgg tcaggttgta ggtccgattc cacttcatga gcagatccac gtacgccagg
     2281 agttgctcgc tgacgccggc aggcagcgcc accgccagct catccaggcc gcgctcgagc
     2341 gcggcgcgcg gcgtcatgcc gtcttcttct gcggaaaacc tcgcttcaga tgaaccagca
     2401 agagcgaaat cgccgccggc gtcaccccag aaatgcgcga cgcctgaccg atcgtttccg
     2461 gccgatggcg gttcagcttt tgctgcacct cggcagacag cccgcgtacc ccgcgatagt
     2521 ccagatcgtt cggcagccgc gtggactcgg cccctttgcg ccgctccacc tcggcgcgct
     2581 ggcgtgagat atagccttcg tacttggctt ggatttcaac ctgctccgcg accgcaggat
     2641 cgctgacggg cgtttccaca atgccgcgat aggtcacctc ggggcgtcga agaagctcgt
     2701 aaatactgct cttttgctga aggccagcgc tcacatatgt ggacttcagc cgttccgtct
     2761 cgccgtcgat cgcatcgcgc ttgcggctaa acgcctccca gcgcgcgtca tcgacgaggc
     2821 caagctcgcg gccgatttcg gtgagacgta gatcggcgtt gtcctcgcgc agggtcagcc
     2881 ggtattcggc ccgactcgtg aacatccgat agggctccga gacaccgctg gtcgtcaagt
     2941 catccacgag gacgcccaga tacccttgat cgcgcttcgg ccaccaaggg tccatgccac
     3001 gaaccttgcg agcggcgttc aacccggcaa gcagaccttg ggccgcggct tcttcgtaac
     3061 ccgtcgtgcc gttgatctga ccggcgaaga acagcccatg gatgctcttg gtttccaggg
     3121 acggccgcag gttacgggga tcgaagtagt cgtattcgat cgcatagcca ggccgaacaa
     3181 tgtgcgcctt ctcgaggccg cgaatggagt gcaccagatc gagctggacg tcgaggggca
     3241 ggctggtcga gatgccgttc gggtagatct cgttggtggt cagcccctcg ggttcgagga
     3301 agatttggtg acttgccttg ccagcaaaac ggtgaatctt gtcctcgatg gaggggcagt
     3361 agcggggacc cactccctga atcacgccag tgaacatcgg cgagcgatcc agccccgcgc
     3421 gaatgatgtc gtgggtgccg gcgttggtat gcgtgatcca gcagggtagc tgctccggat
     3481 gcatgtcccg ccggcctagg aaggagaaca ccggctccgg ccggtcccca ggctggcgct
     3541 ccatcgccga aaaatcgatg cttcgcgcgt cgagccgcgg gggcgtgccg gttttcagac
     3601 ggccgactgg caacttcatc tcacgcaagc gtgcccccaa agtgactgcc gcgggatcgc
     3661 ctgcgcggcc cgcctgatag ctctgcagcc ccacatggat cagccccgac aggaacgtcc
     3721 cggtggtcag gacgatcgtc cggctgcgaa agcgcaggcc ggtttgcgta accacgccaa
     3781 cggcgtgatc cccctcgacc gaaatatcgt caaccgcttg ctggaagatc gcgaggttcg
     3841 gctgattctc gagccgccgt cgaatcgcgc cacggtagag gacgcgatcg gcttgcgcgc
     3901 gggttgcgcg gactgcggcg cctttcgacg agttcaaaat cctgaactga attcccgctt
     3961 cgtctgttgc gagagccatt gccccgccca gcgcgtcgat ttccttgacg agatggcctt
     4021 tgccgatgcc gccgatcgaa gggttgcagg acatctggcc cagggtctct atgctgtggg
     4081 tcagaagcag ggtggcgcac cccatgcgcg ccgcggcgag cgcagcctcc gtgccggcat
     4141 ggccgccgcc gatcactatg acgtcgaatt ctgtgggaaa caacatggag aggcggcgga
     4201 ttttacgcga tggttccacg tggaactagt aacgccacgg ttccacgtgg aacaagttgg
     4261 atgctgcggc gcaacatacg gcggcttggc gccgccggcc aggcggtaac gctaggcggc
     4321 gagcttctgg tgcatcctgc cccggcgcat ctcgagcgcc tcgggcaggc gcgcgccgaa
     4381 ctttccgaac aactcgtcgt gcgctgaaag ttcttcgcgc cacgcaccgt tgtcgactcg
     4441 ctcgagttcg gcgaactggg cgggcgacag cttgtccagc ccggtccagg tcaggtcttc
     4501 atagcgcggc atggtgccca gcggcgtttc tacgccgtgg ccgtggccct ggcagcgctc
     4561 gaccacccac tgcagcactc gcatgttttc gccgaacccc ggccacatga atttgccgtt
     4621 cgcgtcgcgg cggaaccagt tgacgcgaaa gatccggggt ggctgctgca cggtgcgccc
     4681 catcttcagc cagtgagcga agtagtcagc catgttgtag ccgcagaaag
//
LOCUS       DASYJA010000074         3814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3190036, whole genome shotgun sequence.
ACCESSION   DASYJA010000074 DASYJA010000000
VERSION     DASYJA010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3190036"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1066
                     /locus_tag="VGO02_01585"
     CDS             155..1066
                     /locus_tag="VGO02_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEV7476408.1"
                     /translation="MPYSVEGKLVVAISSRALFDFEEENRIFERDGEAAYIALQFARL
                     DVPARHGVAFPLVKKLLAFNAPGQSRVEVVVLSKNDPVSGLRVFKSAQQAGLAIERGV
                     FTRGRNPYEYLEPLKANLFLSANEADVMGALSAAVPAARVYPESAQAASRHAGEVRIA
                     FDGDAVLFSDEAERVFQKDGLDAFTRHETAHALKPLPPGPFKPLLEALHRLQAEKNLP
                     MKIRTALVTARSAPAHERAINTLMNWNIAVDEAMFLGGLEKGAFLKAFEPDFYFDDQR
                     GHIDSARLHVAAGHVPFGIANTAKKQA"
     gene            complement(1079..2275)
                     /locus_tag="VGO02_01590"
     CDS             complement(1079..2275)
                     /locus_tag="VGO02_01590"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEV7476409.1"
                     /translation="MQRKQVTVGELQFGMFVAELDRPWTETPFMFQGFHLRTDQQLAA
                     LRKHCKHVFIDIERTEKNDPRPPAVEFKIRGQTKYVEQVKVEVEYHQATQVYARSIAR
                     IDELLKPISQPGGVLEAKDVKESVTQLTDSVVRNPDAMLLVTRLREKSVAAHARALQV
                     SIYMIVFGRFLELAREELELLGLVGLLQDVGKTRLPASIMEKNTTLTPEEAEIFRRHV
                     LLGAEILKATPGIPPRLLELAILHHERQDGTGYPRGLKGEQIGLYGSIAAIADAFDSL
                     TAQRAKGEPLSPSAALSYLYKERGTGYHPDLVEQFIQCVGVFPVGSVVELNSGEVGLV
                     ITQNLVRRLKPRVMVVLDTKGNPMRPHRILDLDRDPKMSPDEPYRIKRTLEQSKLQVN
                     PKELFI"
     gene            complement(2289..3224)
                     /locus_tag="VGO02_01595"
     CDS             complement(2289..3224)
                     /locus_tag="VGO02_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEV7476410.1"
                     /translation="MSLLELQGVSRDFDVSRPWLNRVIEGEPRRSLRAVDGVSFAVDP
                     GETLALVGESGCGKSTVARLIVGLYAPSEGRIAFRGRRMQMIFQDPYASLNPRWRVQK
                     IVAEPITYLKLEKSPSRVGEKVASLLTQVGLSADDGEKYPHEFSGGQRQRISIARALA
                     GEPDLLVCDEPTSALDVSVQAQILNLMADLQQRIGLTYLFISHNLAVVSQVADRVGVM
                     YLGRIVEIASAVELFAHARHPYTRMLLDAVPDLGMTGKARVPVAGEVPNPLAPPPGCA
                     FHPRCPHANARCRAERPQLIDDVACHAVQEGRITP"
     gene            complement(3211..>3814)
                     /locus_tag="VGO02_01600"
     CDS             complement(3211..>3814)
                     /locus_tag="VGO02_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7476411.1"
                     /translation="KLDRAAARRRALGLLAEVGIPAPEGRFHHYPHQFSGGMRQRVVI
                     ALALAAEPALVIADEPTTALDVSIQAQIIALLKRLAAERGAAVMLITHDMGVIAETAH
                     RVAVMYAGRIVEIGPVREVIHSPQHPYTAGLMGSIPKISLSKAKLVQIDGSMPRLNAI
                     PPGCAFNPRCPKRFARCTLQRPALMAAGTSEAACWLHEPA"
BASE COUNT          608 a         1234 c         1349 g          623 t
ORIGIN      
        1 gggagcggaa gacttctggg tcgagaagtc gcagccggcg agcgcgagcc cggcggcgag
       61 caggagggcg agcatggttt tcattggcgt ctccacagac ggcaagaggc aagcaatccg
      121 ggtgcctgcc ggcaaaagag cgcaaaattg cgctatgccg tattcggtcg aaggcaagct
      181 ggtggtggcg atctcgtcgc gcgcgctgtt cgacttcgag gaggagaacc gcatcttcga
      241 gcgcgacggc gaggcggcct acattgcctt gcagttcgcc cgcctcgacg tgcccgcgcg
      301 gcacggcgtc gccttcccgc tggtgaagaa gctgcttgcc ttcaacgcgc cggggcagag
      361 ccgcgtcgag gtggtggtcc tgtcgaagaa cgacccggtg tcggggctgc gtgttttcaa
      421 atcagcccag caggccgggc tcgccatcga gcgcggcgtg ttcacgcgcg gccgcaaccc
      481 ctacgaatac ctcgagcccc tgaaggcgaa tcttttcctg tcggcgaacg aggccgacgt
      541 gatgggcgcg ctcagcgcgg cggtgccggc ggcgcgcgtc tacccggagt ccgcgcaggc
      601 ggccagccgc cacgccgggg aagtgcgcat cgccttcgac ggcgacgcgg tactgttttc
      661 cgacgaggcg gagcgcgtgt tccagaagga cggcctcgac gccttcacgc gccacgaaac
      721 cgcgcacgcg ctgaagccgc tgccgccggg gccgttcaag ccgctgctcg aggcgctgca
      781 ccgcctgcag gcggaaaaga acctgccgat gaaaatcagg acggccctgg tcacggcgcg
      841 cagcgccccg gcgcacgagc gcgcgatcaa tacgctcatg aactggaaca tcgcggtcga
      901 cgaggcgatg ttcctcggcg ggctggagaa gggcgccttc ctcaaggcct tcgagcccga
      961 cttctacttc gacgaccagc gcggccacat cgactcggcg cgcctgcacg tcgctgcagg
     1021 ccacgtgcca ttcggtatcg caaataccgc taagaagcag gcgtagccaa cctgcgctct
     1081 aaatgaagag ctctttcggg ttgacctgca gcttgctctg ctcgagggtg cgcttgatgc
     1141 ggtagggctc gtcgggcgac atcttggggt cgcggtcgag gtcgagaatc ctgtgcgggc
     1201 gcatcgggtt gcccttggtg tcgagcacca ccatcacccg cggcttgagc cgccgcacca
     1261 ggttctgcgt gatcaccagc ccgacctcgc cggaattgag ctcgaccacg gagccgaccg
     1321 ggaacacgcc gacgcactgg atgaactgct cgaccaggtc ggggtgatag ccggtgccgc
     1381 gctccttgta gaggtagctg agcgcggcgg aaggcgacag cggctcgccc ttggcgcgct
     1441 gcgcggtgag gctgtcgaag gcgtcggcga tggcggcgat cgagccgtac agcccgatct
     1501 gctcgccttt caggccgcgc gggtagccgg tgccgtcctg gcgctcgtgg tgcaggatgg
     1561 cgagctccag cagccgcggc gggatgccgg gggtggcctt gaggatctcc gcgccgagca
     1621 ggacgtgccg cctgaagatc tcggcctcct cgggtgtgag cgtggtgttc ttctccatga
     1681 tcgaggcggg cagcctggtc ttgccgacgt cctgcagcag gccgaccagg ccgaggagct
     1741 ccagctcctc gcgcgccagc tcgaggaagc ggccgaatac gatcatgtag atcgagacct
     1801 gcagcgcgcg cgcgtgcgcg gccacgctct tctcgcgcag gcgcgtcacc agcagcatgg
     1861 cgtcggggtt gcgcaccacg ctgtcggtaa gctgcgtcac cgactccttc acgtccttcg
     1921 cctcgagcac gccgccgggc tgggagatcg gcttgagcag ctcgtcgatg cgggcgatgc
     1981 tcctggcata gacctgggtc gcctggtggt actcgacctc gaccttgacc tgctcgacgt
     2041 acttggtctg gccgcggatc ttgaactcga ccgccggcgg ccgcggatcg ttcttctcgg
     2101 tgcgctcgat gtcgatgaag acgtgcttgc agtgcttcct cagcgccgcg agctgctggt
     2161 cggtgcgcag gtggaagccc tggaacatga acggcgtctc ggtccacggc cggtcgagct
     2221 ccgcgacgaa catgccgaac tgcagctccc ccaccgtgac ctgttttcgc tgcatgtaac
     2281 gcaggatttt aaggcgttat gcgtccttct tggacggcat ggcaagcgac gtcgtcgatg
     2341 agttgcgggc gctcggcgcg gcagcgcgcg ttggcgtggg ggcagcgagg gtggaaggcg
     2401 cagcccggcg ggggcgccag ggggttcggc acctcgccgg ccaccggcac gcgcgccttg
     2461 cccgtcatgc cgaggtcggg caccgcgtcg agcagcatgc gggtatacgg atggcgtgcg
     2521 tgcgcgaaca attccacggc agaggcgatc tcgacgatgc ggccgaggta catgacgccg
     2581 acccggtcgg cgacctggga caccaccgcg aggttgtgcg agatgaaaag gtaggtgagc
     2641 ccgatgcgct gctgcaggtc ggccatcagg ttgaggatct gcgcctgcac cgagacgtcg
     2701 agcgccgagg tcggctcgtc gcacaccagc aggtcgggct cgccggcgag ggcgcgcgcg
     2761 atcgagatgc gctggcgctg cccgccggag aattcgtgcg ggtacttttc gccgtcgtcc
     2821 gccgacaggc cgacctgcgt gagcaggctt gcgacctttt ctcccacgcg ggaaggtgat
     2881 ttttccaact tcaaatacgt gatgggctcg gcgacgatct tttgcacgcg ccagcgcgga
     2941 ttgaggctgg cgtagggatc ctggaagatc atctgcatgc gccggccgcg gaaggcgatg
     3001 cgcccctcgc tcggcgcgta cagaccgacg atgaggcgcg ccacggtgga cttgccgcag
     3061 cccgactcgc ccaccagcgc gagggtctcg ccggggtcga cggcgaagct cacgccgtcg
     3121 accgcgcgca ggctgcggcg cggctcgccc tcgatcaccc ggttgagcca gggccgcgac
     3181 acgtcgaagt cgcgacttac cccttgcagt tcaagcaggc tcatgtaacc agcatgcagc
     3241 ctcgctcgtt cccgccgcca tcagcgcggg acgttgcagc gtgcagcgcg cgaagcgttt
     3301 cgggcagcgc gggttgaagg cgcagcccgg cggaatggcg ttgagccgcg gcatcgagcc
     3361 gtcaatctga acaagttttg cttttgaaag agaaatcttg ggtatggagc ccatgaggcc
     3421 ggccgtgtac gggtgctgcg gcgaatggat cacctcgcgc accgggccga tctcgacgat
     3481 gcgcccggca tacatgaccg ccacgcggtg cgcggtctcg gcgatgacgc ccatgtcatg
     3541 cgtgatcagc atcaccgcgg cgccgcgttc cgccgccagg cgcttgagca gcgcgatgat
     3601 ctgcgcctgg atcgacacgt cgagcgcggt ggtcggctca tcggcgatca ccagcgccgg
     3661 ctcggcggcg agcgcgagcg cgatgaccac acgctggcgc atgccgcccg agaactggtg
     3721 ggggtagtgg tggaagcggc cctccggggc gggaatgccg acctccgcga gcagcccgag
     3781 ggcgcgccgg cgcgctgcgg cgcgatccaa cttc
//
LOCUS       DASYJA010000075         4624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1555781, whole genome shotgun sequence.
ACCESSION   DASYJA010000075 DASYJA010000000
VERSION     DASYJA010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555781"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..2517)
                     /gene="lon"
                     /locus_tag="VGO02_01605"
                     /pseudo
     CDS             complement(169..2517)
                     /gene="lon"
                     /locus_tag="VGO02_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
     gene            complement(2645..3916)
                     /gene="clpX"
                     /locus_tag="VGO02_01610"
     CDS             complement(2645..3916)
                     /gene="clpX"
                     /locus_tag="VGO02_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEV7476412.1"
                     /translation="MSEKSSSGEKLLYCSFCGKSQHEVRKLIAGPSVFICDECIELCN
                     DIIREETASDKGGKGAKSDLPTPREIRNILDSYVIGQDTAKKILSVAVYNHYKRLKHL
                     SKNDEVELAKSNILLIGPTGSGKTLLAQTLARLLNVPFVIADATTLTEAGYVGEDVEN
                     IIQKLLQNCDYDVDKAKRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLVEGT
                     IASVPPQGGRKHPNQDFVQVDTTNILFICGGAFDGLEKIVRNRTEKTGIGFGAEVKSP
                     KNSKATYDLLKSVEPEDLIKYGLIPEFVGRLPVLATLEELDEHALIQILTEPKNALIK
                     QYQRLFLMEGVELEVRVPALGAVAKRALARKTGARGLRSILEQILLDTMYELPTLENV
                     SKVVVDESMVSADGKPLLIYSDQHKVAGSAG"
     gene            complement(3921..4589)
                     /gene="clpP"
                     /locus_tag="VGO02_01615"
     CDS             complement(3921..4589)
                     /gene="clpP"
                     /locus_tag="VGO02_01615"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEV7476413.1"
                     /translation="MPSHIEYQRYREFLHDTGRGALDTQSLGLIPMVIEQSGRGERAY
                     DIYSRLLKERVVFLVGPVTEVTANLIVAQLLFLESENPDKDIFFYINSPGGSVSAGLA
                     IYDTMQFIKPDVSTLCVGQAASMGATLLAAGDKDKRFCLPNSRVMIHQPSGGFQGQVS
                     DIEIHAKEILYLKSRLNEILARHTGKTIEAIAHDSDRDFFMGAEDAVKYGIVDKVLTA
                     RENT"
BASE COUNT          716 a         1459 c         1467 g          982 t
ORIGIN      
        1 ccgcgatgta gcggtgtcaa gcaacctccc cgcactgcgc actcgcaaaa aaaatcgggc
       61 ggcgcccaag cgccgcccga gtcctggagc gtttctaaat cagtgcgtaa cgacctgggg
      121 cgatgacttg tcgtcaccca ccggcggcag cgcggccacc ggatcggcga gcggctcggg
      181 catgcgctcg agcgccttct ccagcacctg gtcgatccac ttctccggca ggatctcgag
      241 ctggttcttg atgttgtccg ggatgtcgac caggtctttc acgttctctt ccgggatgat
      301 caccgtcttg atgccgccgc ggtgcgccgc cagcagcttc tccttcaggc cgccgatctg
      361 cagcacctcg ccgcgcagcg tgatctcgcc ggtcatggcg acgtccgcgc gcaccggaat
      421 gccggtcagc acggagacca tcgccgtggt gatggcgccg cccgcgctcg ggccgtcctt
      481 gggcgtcgcg ccctcgggca ggtgaatgtg gatgtcgttc ttctggtaga agtcttcctt
      541 cacgccgagc ttgcgcgagc gcgcgcgcac cactgaaagc gcggcctgga tcgactcctg
      601 catcacgtcg ccgagcttgc cggtggtgat ggtcttgccc ttgccgggca tggtcaccgt
      661 ctcgatggtc agcagctcgc cgccgacttc ggtccaggcc aggccggtaa cctgcccgac
      721 ctggttctgc ttctccgcca tgccgaagct gtacttgcgc accccgaggt acttgtccag
      781 gttcttcggc gtcaccgtga tgcggtgcgc cttctcgtcg ggcttcttgt cggtcagcag
      841 ctgtttcacc gccttgcgac agatcttgga aacttcgcgt tcgagagcac gcacgcccgc
      901 ttcccgcgtg tagtaccgaa cgatgtcgcg gacggccggc tccgacaccg agatctcgcc
      961 ttccttgagc ccgttcgcct tgagctgctt gggcagcagg tagcgaatgg cgatgttcac
     1021 cttctcgtct tccgtgtagc cggagaggcg gatgacctcc atgcggtcga gcagcggcgc
     1081 cgggatgttc agcgtgttcg cggtagcgac gaacatcact tccgacaggt cgtattcgac
     1141 ctcgatgtag tggtccgtga aggtgttgtt ctgctccggg tccagtactt cgagcaaggc
     1201 gctggacggg tcgccgcgcc agtcggcgcc catcttgtcg acctcgtcga gcaggaacag
     1261 cgggttcttc accgccacct tggtcatgtt ctgcaggatc ttgccgggca tcgagccgat
     1321 gtaggtgcgc cggtggccgc ggatctcggc ctcgtcgcgc accccgccga gcgacatgcg
     1381 cacgaacttg cggttggtgg cgcgggcgat cgactggccg agcgaggtct tgccgacgcc
     1441 gggcgcgccc accaggcaga ggatcggcgc cttcatcttg tcgacgcgcg tctgcaccgc
     1501 caggtactcg acgatgcgct ccttcacctt ctccaggccg tagtggtcct tgtcgaggat
     1561 ctcctgcgcg gccttgatgt ccttcgagat cttcgacttc ttgcgccacg gcaggttgac
     1621 cagggtctcg atgtagttgc gcacgacggt ggcttccgcc gacatcggcg acatcagctt
     1681 cagcttcttc agctcggact ccgccttctt gcgcgcgtcc ttgtgcatgt gcgccttgcg
     1741 gatcttcttc tccatctcct cgaggtcggc gccctcctcg ccctcgccca gctccttctg
     1801 gatcgccttc acctgctcgt tgaggtagta ctcgcgctgg ctcttctcca tctgccgctt
     1861 gacgcggccg cggatgcgct tctccacctg caggatgtca atctcggtct cgagctgcga
     1921 cagcaggtgc tccaggcgct gcttgacgtc gaacatctcg agcacctgct gcttctgctc
     1981 gagcttgagc ggcaggtgcg cggcgacggt gtcggccagg cggccggcct cgtcgatgcc
     2041 ggagagcgag gtcaggatct cgggcggaat cttcttgttc agcttgacgt actggtcgaa
     2101 cgcggaaagc agcgcgcggc gcatcgcctc gacttccggc gccggggtcg ggtcgagctg
     2161 caccagcgag gcgtcggcca cccagtgcgt gcgcacatcg gtgatctcgt ggatgcgcgc
     2221 gcgcgtgccg ccctcgacca gcaccttcac cgtgccgtcg ggcagcttca gcatctgcag
     2281 gatgttggag acgcagccga tcgcgtacat gtcgcccgga cccggatcgt ccttggcggc
     2341 ggatttctgc gccaccagca ggatcgactt gccggcttcc atcgccgttt ccatcgcctt
     2401 gatcgacttc ggccgcccga cgaacagcgg gatcaccatg tgggggaata ccaccacgtc
     2461 ccgtaggggc agaagcgggt actggaggct gtcggaaggg gcaggcgttt ctgacattta
     2521 gcttagcctt tcaagcagtt ggactgcccg ccttaagtgc gggttcctgc cggaaaatca
     2581 atgcacaaag cgtacacgga aacccgtgca cgctgcaatg cagaaaatat taaagattaa
     2641 gaatctatcc ggcggagccg gcgactttgt gctggtcgga atagatcagc agcggcttgc
     2701 cgtcggcgct gaccatcgac tcgtcgacca cgaccttgct gacgttctca agggtgggca
     2761 gctcgtacat cgtgtcgagc aggatctgct ccaggatcga gcgcaggccg cgcgcgccgg
     2821 tcttgcgcgc gagcgcgcgc ttggcgacgg cgccgagcgc cgggacgcgc acctcgagct
     2881 ccacgccctc catcaggaac aggcgctggt actgcttgat gagcgcgttc ttcggctcgg
     2941 tgaggatctg gatcagcgcg tgctcgtcta gctcctcgag cgtcgccaga acgggcaggc
     3001 gtccgacgaa ctccggaatc agcccgtact tgatcaggtc ttcgggctcc acgctcttca
     3061 ggagatcgta cgtggccttc gaattcttcg ggctcttcac ctcggcgccg aagccgatgc
     3121 cggtcttctc ggtgcggttg cgcacgattt tctcgaggcc gtcgaaggcg ccgccgcaga
     3181 tgaacaggat gttggtggtg tcgacctgca cgaagtcctg gttcgggtgc ttgcggccgc
     3241 cctgcggcgg aacggaggca atcgtcccct ctaccaattt caaaagggct tgctgaacac
     3301 cttcacccga gacgtcgcgg gtgatcgagg ggttgtccga cttgcgcgag atcttgtcga
     3361 tctcgtcgat gtagacgatg ccgcgcttcg ccttgtcgac gtcgtagtcg cagttctgca
     3421 gcagcttctg gatgatgttc tcgacgtcct cgccgacgta gcccgcctcg gtgagcgtgg
     3481 tggcgtcggc gatcacaaaa ggaacattca gcaatcttgc caaggtctgg gcaagcagcg
     3541 ttttgccgga gccggtcggc ccgatcagca ggatgttgga cttggcgagc tcgacctcgt
     3601 cgttcttgga caggtgcttg agccgcttgt agtggttgta gaccgcgacc gagaggatct
     3661 tcttggcggt gtcctgcccg atcacgtacg agtcgaggat gttgcggatc tcgcgcggcg
     3721 tcggcaggtc ggacttggcg cccttgccgc ccttatcgga ggcggtctcc tcccggatga
     3781 tgtcgttgca cagctcgatg cattcgtcgc agatgaacac cgacgggccg gcaatcagct
     3841 tcctgacctc gtgctggctc ttgccgcaga acgagcaata cagcagcttc tcgccggaag
     3901 aagacttttc cgacataggg ttaggtgttc tcccgggcgg tcaggacctt gtccactatg
     3961 ccgtacttta ccgcgtcttc ggctcccatg aaaaagtcgc ggtcgctgtc gtgggcgatg
     4021 gcctcgatgg tcttgccggt gtgcctggcg aggatctcgt tcagccggga cttgagatag
     4081 aggatttcct tggcgtggat ctcgatgtcc gacacctggc cctggaagcc gcccgagggc
     4141 tggtggatca tgacgcgcga gttgggcagg cagaagcgct tgtccttgtc gccggcggcg
     4201 agcagcgtcg cccccatgct ggccgcctgc ccgacgcaca gcgtcgagac gtccggcttg
     4261 atgaactgca tggtgtcgta gatcgccagg ccggccgata ccgagccgcc gggcgagttg
     4321 atgtagaaga agatgtcctt gtcgggattc tccgactcga ggaacaggag ctgggcgacg
     4381 atcaggttgg cggtgacttc ggtcaccggg ccgaccagga atacgactct ttctttcaac
     4441 agcctggaat aaatgtcgta cgcgcgctct ccgcgccccg actgctcgat caccatcggg
     4501 atcaggccga gcgactgggt atcgagcgcg ccgcgcccgg tatcgtggag gaattcgcgg
     4561 taacgctggt attcgatgtg cgacggcatg ctttgagttc ctgtttcctt gcctgtttag
     4621 ctgt
//
LOCUS       DASYJA010000076         5783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1374456, whole genome shotgun sequence.
ACCESSION   DASYJA010000076 DASYJA010000000
VERSION     DASYJA010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374456"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..761
                     /locus_tag="VGO02_01620"
     CDS             138..761
                     /locus_tag="VGO02_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV7476414.1"
                     /translation="MSRLFAAMAALALASCAQAPQQAPTQDAITVVKSLQAGGYVIVF
                     RHGATNRDMADTDPLNLDNVAQQRQLSERGRQVARDVGAAFKTLQIPIGQVYTSRFYR
                     AVETGRLVGGKEVIATADVSEGGQVVTPIENDRRAAAFRKLAATPPAPGTNTLIVSHK
                     PNILDAFGKDWFDVREGEASVFRPEAGKYVLVAKLQAAEWLDLAATR"
     gene            complement(758..1579)
                     /locus_tag="VGO02_01625"
     CDS             complement(758..1579)
                     /locus_tag="VGO02_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV7476415.1"
                     /translation="MKQVLLPAGERMPAFGMGTWYMGEKRSERPAEVAALRLGLDLGI
                     RLIDTAEMYGEGGAEEVVGEAIAGRRDEVFLVSKVYPHNASRQGAVAACERSLKRLKT
                     DRLDLYLLHWRGNIPFAETVAAFEALRAAGKIRHWGVSNLDLADMQELGSAPVQTDQL
                     LYNLTRRGIEWDLLPWLRERRIPVMAYSPIEQARLLKSRALVDFSKKHGMTPAQAALA
                     WLLAKDDVIVIPKASNPEHLKENLGALKIQLTAAQLAELDRAFPPPKGPSPLEML"
     gene            complement(1576..4452)
                     /locus_tag="VGO02_01630"
     CDS             complement(1576..4452)
                     /locus_tag="VGO02_01630"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEV7476416.1"
                     /translation="MREPHLSELRRDHRIAEAEAVARLRALQPDAGLSRRIHEKALRL
                     AQAVRSTRPATLSAESFLRHYGLSTPEGVALMCVAEALLRIPDAATADALLREKLSSG
                     NWSTASAADWALMLTGTLVSWHDAPGEIRRVVARLGEPVVRAAVKQAMRILAEQFVLA
                     ETIEAAVARGRQRRPYRFSFDMLGEAARTAEDAARYLELYARAIGAVAAPDGVSIKLS
                     ALHPRFEEAKRARVFSELVPRLENLAALAVARGVDLYIDAEESERLELTLDLHESLSK
                     KFPMGLAVQAYQKRAFAVCDWLGSLGRKIPVRLVKGAYWDSEIKRAQQLGMPDYPVFT
                     RKSATDVSYVACATRLLSSPTIFPAFATHNCRTVATVLELAGDRQFEFQKLHGMGDAL
                     YQSLMKEHRVNVRVYAPVGSFTDLLPYLVRRLLENGANTSFVHQIADPDVPVEELIAD
                     PVAALPEPYVPDPRIPLPRDLYPDRKNSLGLDLSRRDVLDSLHATINADKLEPIATAP
                     SLDESVRRATGAFDSWSRVPVAERAATLERAADALEAGMIELVSLIVREGGRTYADAV
                     SEVREAADFCRYYALQARRVFTETVLAGPAGERNQLSLHGRGVFACIAPWNFPLAIFT
                     GQVAAALAAGNSVIAKPAEQTPRVGLRAIEILRASGIPENAVHCAPGDGESVGAPLVR
                     DPRIAGVAFTGSDETARRIHTALAARPGPIVPLIAETGGVNAMLADSSALPEQVVDDV
                     VVSAFQSAGQRCSAQRILFVDEGAAPRVLRLLAGALAELRIGDPSEPDTDIGPVIDAA
                     ARDALLRHIGNLRKKAVLIGESRVPGSGNYVAPIAFEMKLRDLPTVEVFGPVLHVCTY
                     ARQDLAEVLRWLRDTGYGLTLGIHSRLNSFVDEVVREARVGNIYVNRSMIGAVVGVQP
                     FGGEGLSGTGPKAGGPHYLPRFATERTLTVNTAAIGGVTELFSK"
     gene            4593..>5783
                     /locus_tag="VGO02_01635"
     CDS             4593..>5783
                     /locus_tag="VGO02_01635"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HEV7476417.1"
                     /translation="MAATALTALPGCAAQGTRPQLSFTPIAPSSDDLVRVPPGYEAKV
                     LYRWGDPVGAAAGMPAFRMDGSNSAADQALQAGMHHDGMHYFPLDGSRRGLLAMNHEY
                     LDEGLLFTDGMKTWSAEKVLKAQHAVGVSVIEVVQEKNQWEVVRPSKYARRITARTPC
                     RVGGPAAGHSLMRTANDPGGTIIHGTYAGCAHGWTPWGTYLTCEENWHIYFVNGGAIS
                     ADQQRYSITPKGRNTRWQEHDERFDAARHPNELNRFGWVVEIDPFDPDSIPVKRTALG
                     RIKHEGAACSVGADRRLAFYMGDDEDGEYIYKFVTERPFDPQNRKANADLLDDGKLYA
                     ARFNPDGSGDWLELSGPDLGAVRVRTRQAADAAGATGVNRAEWVVPHPVTREVFASLT
                     GGKGN"
BASE COUNT          942 a         1949 c         2010 g          882 t
ORIGIN      
        1 gcggctgccc ggcgagggcg cgaagttcgc gcccaacacg cccgagcagt tctcgaaatt
       61 cgtggtcgac gagatcgcga agtgggcgcc ggtagtcagg gcatccggtg cgagggtgga
      121 ctaacaggga gaaaccgatg agcaggctct tcgcagcaat ggcggcgttg gcgctggcga
      181 gctgcgcgca ggcaccgcag caggcgccga cgcaggacgc gattacagtc gtgaagtcgc
      241 ttcaggcggg cggttacgtg atcgtgttcc ggcacggcgc gaccaatcgc gacatggcgg
      301 acaccgatcc gctcaacctc gacaacgtcg cccagcagcg ccagctgagc gagcggggcc
      361 gacaggtggc gcgcgatgtc ggagcggcgt tcaagaccct gcagattccg atcgggcagg
      421 tctatacgag ccggttttac cgcgccgtcg aaaccggccg cctggtgggc ggcaaggaag
      481 tgattgccac cgcggacgtc tcggaaggcg gccaggtggt gacgccgatc gagaacgacc
      541 gccgcgcggc ggccttccgc aagctggcgg cgacgccgcc ggcgccgggc acgaacacgc
      601 tgatcgtctc gcacaagccg aacatcctgg acgcgttcgg caaggactgg ttcgacgtgc
      661 gcgagggaga ggcgtcggtc ttcaggccgg aagccggcaa atacgttctg gtcgccaagc
      721 tgcaggcggc cgagtggctg gacctggcgg cgacgcgcta gagcatttcc agcgggctcg
      781 ggcctttcgg cggcgggaac gcccggtcga gctcggcgag ctgcgccgcc gtgagctgga
      841 tcttcagtgc gcccagattt tccttcaggt gctccggatt ggaggccttc ggaatgacga
      901 tgacgtcatc cttcgccagc agccaggcga gcgcggcctg ggccggcgtc attccatgct
      961 tttttgagaa gtcgacaagc gcgcggctct tcagcagccg cgcctgctcg atcggcgagt
     1021 aggccatcac cgggatgcgc cgctcgcgca gccagggcag cagatcccac tcgattcccc
     1081 ttcgcgtcag gttgtagaga agctggtcgg tctgcaccgg cgccgaaccg agctcctgca
     1141 tgtcggcgag gtcgaggttg ctcacgcccc agtgccggat cttgccggcg gcgcgcagcg
     1201 cctcgaaggc cgccacggtt tcggcgaacg gaatgttgcc gcgccaatgc agcaggtaga
     1261 ggtcgaggcg atcggtcttc agccgcttca ggctgcgctc gcatgcggcg accgcgcctt
     1321 ggcgcgaggc gttatgcgga taaaccttgc tgaccaggaa tacctcatcg cgccgcccgg
     1381 cgatcgcttc gcccaccact tcctcggcgc cgccttcgcc gtacatctcg gcggtgtcga
     1441 tcaggcggat gcccagatcg agccccagcc tgagcgccgc gacctcggcg gggcgctcgc
     1501 tgcgcttctc tcccatgtac caggtgccca tgccgaaggc gggcatgcgc tcgccggccg
     1561 gcaggaggac ttgcttcatt tcgaaaagag ctccgtcacg ccgccgatgg ccgccgtgtt
     1621 caccgtgagc gtgcgctcgg tggcgaagcg cggcaggtag tgcggcccgc cggccttcgg
     1681 cccggtgccc gacaggcctt cgccgccgaa gggctgcacg cccaccaccg cgccgatcat
     1741 gctgcggttc acgtagatat taccgacgcg cgcctcgcgc accacctcgt cgacgaagct
     1801 gttcagccgg ctgtggatgc cgagggtcag cccgtagccc gtgtcgcgca gccaccgcag
     1861 gacttctgcc aggtcctgcc gggcataggt gcacacatgc aggaccggcc cgaacacttc
     1921 caccgttggc aggtcgcgca acttcatttc aaaggcgatc ggcgcgacgt agttgccgct
     1981 cccaggtacc ctggattcgc cgatcagcac cgcctttttc ctcaggttcc cgatatggcg
     2041 cagcagcgca tcgcgcgccg ccgcatcgat gaccgggccg atgtcggtgt cgggctcgga
     2101 cgggtcgccg atcctgagct cggctagcgc gccggcgagc aggcgcagca cgcgcggcgc
     2161 ggcgccctcg tcgacgaaca ggatgcgctg cgccgagcag cgctggccgg cgctctggaa
     2221 cgcggacacg acgacgtcgt ccaccacctg ctcgggcagc gccgaggagt cggccagcat
     2281 cgcattgacg ccgccggtct cggcgatgag cggcacgatg ggtcccgggc gcgcggcgag
     2341 cgccgtgtgg atgcgccgcg cagtctcgtc ggacccggtg aaggccacac cggcgatgcg
     2401 cgggtcgcgc accagcggtg cgccgacgct ttcaccgtcg cccggcgcgc aatgcaccgc
     2461 gttttctgga atgccgctcg ccctgaggat ctcgatcgct ctcaggccga cgcgcggcgt
     2521 ctgctcggcg ggcttcgcaa tgacgctgtt gcctgcggcc agcgcggccg cgacctgccc
     2581 ggtgaagatg gcgagcggga agttccatgg cgcgatgcag gcgaacacgc cgcggccatg
     2641 aagggaaagc tggtttcgct cgccggcggg tcccgcaagg acagtttccg taaagaccct
     2701 ccgcgcctgc aaggcgtagt agcggcagaa atccgctgcc tcgcgcacct cggataccgc
     2761 gtcggcatag gtgcggccgc cttcgcgcac gatgagcgag acgagctcga tcatgcccgc
     2821 ctcgagggcg tcggccgcgc gctccagcgt cgcggcgcgc tcggccactg ggacgcgcga
     2881 ccacgagtcg aacgcacctg ttgccctccg gacgctttca tccagggatg gagcggttgc
     2941 aatcggctcc agcttgtcag cgttgatcgt tgcgtgaagg gaatcgagaa cgtcacggcg
     3001 agacaggtcc aggccgaggg agttcttccg gtcgggatac aggtcgcgcg gcagcgggat
     3061 gcgcggatcg gggacatagg gctcgggcag ggcggcgacc gggtcggcga tcagctcctc
     3121 gacgggcacg tccggatcgg cgatctggtg cacgaacgag gtgttggcgc cgttctcgag
     3181 cagccggcgc acgaggtagg gcagcaggtc ggtgaagctg ccgaccgggg cgtagacgcg
     3241 cacgttgacc cggtgctcct tcatcagcga ttgatagagc gcgtcgccca tgccgtgcag
     3301 cttctggaac tcgaactgcc ggtcgcccgc cagctcgaga accgtcgcca ccgtgcggca
     3361 gttgtgcgtg gcgaacgccg gaaagatcgt cggcgaggaa aggagccgcg tggcgcacgc
     3421 gacataagac acgtcggtcg cgctcttgcg cgtgaacacc gggtagtcgg gcatgccgag
     3481 ctgctgcgcg cgtttgattt cgctgtccca gtacgcgccc tttacgagcc gcaccggaat
     3541 cttgcgcccc aggctgccga gccagtcgca gaccgcgaag gcgcgtttct gatacgcctg
     3601 caccgcgagt cccatcggga atttcttcga aagggattca tgcaggtcca gggtcagctc
     3661 gagccgctcc gattcctcgg cgtcgatgta gaggtcgacg ccgcgcgcca cggccagggc
     3721 cgcgaggttt tcaagtcttg gaacaagttc ggagaagacg cgcgcgcgct tcgcttcctc
     3781 gaagcggggg tgcagcgcgg agagcttgat cgagacgccg tcgggcgcgg cgaccgcgcc
     3841 gatggcgcgc gcgtagagct cgaggtagcg cgccgcgtct tccgcggtgc gcgccgcctc
     3901 gccgagcatg tcgaaggaga agcggtaggg ccggcgctgc ctgccgcgcg ccacggcggc
     3961 ctcgatcgtt tcggcgagca cgaactgctc ggcgaggatg cgcatcgcct gcttgaccgc
     4021 ggcgcgcacc accggctcgc cgaggcgggc gacgacgcgc ctgatctcgc cgggcgcatc
     4081 gtgccagctg accagggtgc cggtgagcat gagcgcccag tccgcggcgc tggcggtgct
     4141 ccagttcccc gacgacagct tctctctcaa cagcgcgtcg gccgttgccg cgtccgggat
     4201 gcgcagcagc gcctccgcca cgcacatcag cgcaaccccc tcgggagtgg acaggccgta
     4261 atggcgcagg aacgactccg cgctcagcgt cgccggccgg gtgctgcgca ccgcctgcgc
     4321 gagccgcagc gctttctcgt ggatccgccg cgacagtccg gcgtcaggtt gcagcgcgcg
     4381 caggcgcgcc acggcctcgg cctccgcgat gcgatggtcg cgcctgagtt cagagagatg
     4441 cggttcccgc acgggcccta taatgctgcg cgaaaaggcg gagaggcacc accatgagtc
     4501 gcaatttgca tttgccgggc cgttgcaatc cgagtgccaa cccgtcgttc gaagaggttc
     4561 tcctttcgag aaggaccctg ctcaaggcag gcgtggccgc cacggcgctc accgcgctgc
     4621 cggggtgcgc cgcgcaaggc acccgcccgc agctcagctt cacgcccatc gcgccctcca
     4681 gcgacgacct ggtgcgcgtg ccgccgggat acgaggcgaa agtgctctac cgctggggcg
     4741 acccggtcgg cgccgcggcc ggcatgccgg cattccgcat ggacggctcg aacagcgccg
     4801 ccgaccaggc gctgcaggcg ggcatgcacc acgacggcat gcactatttc ccgctcgacg
     4861 gctcgcggcg cggactgctc gccatgaacc acgagtacct ggacgagggg ctgcttttta
     4921 ccgatggcat gaagacctgg agcgccgaga aggtgctgaa agcgcagcac gccgtcggcg
     4981 tttcggtcat agaagtggtg caggaaaaga accagtggga agtcgtcagg ccgtcgaaat
     5041 acgcccgccg catcaccgcg cgcacgcctt gccgggtcgg cgggccggcc gccggccatt
     5101 cactgatgcg cacggcgaac gaccctggcg gcacgatcat ccacggcact tacgccggct
     5161 gcgcgcacgg ctggacgccg tggggcacct accttacctg cgaagagaac tggcacatct
     5221 acttcgtgaa tggcggcgcg atttccgccg accagcaacg ctatagcatc accccgaagg
     5281 gcaggaatac ccgctggcag gagcacgacg agcgcttcga tgcagcaagg catcccaacg
     5341 agctgaaccg cttcggttgg gtggtggaga tcgacccttt cgatcccgac tcgattccgg
     5401 tcaagcgcac cgcgctcggc cgcatcaagc acgagggcgc ggcctgctcg gtcggcgccg
     5461 accggcggct cgccttctac atgggcgacg acgaggacgg cgagtacatc tacaagttcg
     5521 tcaccgagcg gcccttcgac ccgcagaacc gcaaggccaa cgccgacctg ctcgacgacg
     5581 gaaagctgta cgcggcgcgt ttcaatccgg acggcagcgg cgactggctc gagctgtccg
     5641 gccccgatct cggtgcggtg cgcgtgcgca cccggcaggc ggcggacgcg gcgggcgcga
     5701 ccggcgtcaa ccgggccgaa tgggtagtgc cgcatccggt gacgcgcgag gtattcgcat
     5761 cgcttaccgg cggcaaggga aat
//
LOCUS       DASYJA010000077         2436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1918914, whole genome shotgun sequence.
ACCESSION   DASYJA010000077 DASYJA010000000
VERSION     DASYJA010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918914"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2424)
                     /locus_tag="VGO02_01640"
     CDS             complement(<1..2424)
                     /locus_tag="VGO02_01640"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV7476418.1"
                     /translation="MQKRSFDILLVVSLVSPGNARDTLFLSNYASINLVDELKRLPGI
                     ADVTIFGARDYSMRVWLNPEKMARLGITTADVANALRAQNAQYAAGRIGTEPAPAGQS
                     IVYTVTATGRLVEPEEFGEIVVRASGQPGGILRLKDIARLELGALNYDTSNTLDGQPT
                     IGMATYLQPGSNALEVAALVRKRMTELRPAFPPGVDYAIPFDTTRFVDASIKEVNLTI
                     FEAALLVLAVVFLFLQTWRATLIPMIAVPVSLIGAFAGLWLVGFSINTLTLFALVLAI
                     GIVVDDAIVVLENVERLMRTRGMPPFEAALEAMREVSGAVIAIVLVLCAVFIPVAFLG
                     GIAGALYRQFAVTLSFAVVISGFIALTLTPALCAIMMRAGEHESRVFRPFNRGFDWVT
                     QRFLGAVQVLLRHPAISLVGFVLLLATCVFLFMRVPSSFVPQEDQGYIFGNIQLPDGA
                     TLERTRNMAADLGKIIQANPGVQNTMVINGFDLLGGGNKTNTSTMFIPLKHWDQRKNA
                     PAAAVARDIAAKGSQLREGITIAFNPAAIRGLGTAGGFEMYLQATADADPARLYEATQ
                     TFLAALREHPQLTAINSFFRPIVPQLRVEVDREKAMSLGVPVQDVFDALQSTMGALYV
                     NDFNKFGRTYRVQMMADAPYRSQPEDLGAVYVRSGMSREMIPLKALIRVNNTVGAEQL
                     ERFNGFLAARVLGSGRPGVSSGEAIRAAEETAARVLPEGYQIAWSGQAFQEKRTGRQS
                     VIAFTLAIVMVFLILAALYERWLLPFAVVLAVPFAVFGALSFVWARGLENDIYFQIGL
                     VVLIGLAAKN"
BASE COUNT          421 a          795 c          859 g          361 t
ORIGIN      
        1 gttcttcgcg gccaggccga tcagcaccac caggccgatc tggaagtaga tgtcgttctc
       61 caggccgcgc gcccagacga aggagagcgc gccgaacacc gcgaagggca ccgccagcac
      121 caccgcgaag ggcagcagcc agcgctcgta gagcgcggcg aggatcagga acaccatgac
      181 gatggcgagc gtgaaggcga tcaccgactg gcgcccggtg cgcttctcct ggaacgcctg
      241 gcccgaccag gcgatctggt agccctctgg cagcacgcgc gccgcggtct cttccgcggc
      301 gcggatcgcc tcgccggagg agacgccggg cctgccgctg cccagcacgc gcgcggcgag
      361 gaagccgttg aagcgctcga gctgctccgc gccgacggtg ttgttgacgc ggatcagcgc
      421 cttgagcggg atcatctcgc gcgacatgcc cgagcgcaca tagaccgcgc cgaggtcctc
      481 gggctgcgag cggtagggcg catcggccat catctgcacg cggtaggtgc ggccgaactt
      541 gttgaagtcg ttgacgtaca gcgcgcccat cgtgctctgc agcgcgtcga acacgtcctg
      601 caccggcacg ccgagcgaca tggctttctc gcggtcgacc tccacgcgca gctgcggcac
      661 gatgggacgg aagaacgagt tgatcgccgt gagctgcgga tgctcgcgca gcgccgcgag
      721 gaaggtctgc gttgcctcgt acaaccgcgc cgggtcggcg tcggcggtgg cctgcaggta
      781 catctcgaag ccgccggcgg tgcccaggcc gcggattgcc gcggggttga aggcgatggt
      841 gatgccctcg cgcagctgcg agcccttggc ggcgatgtcg cgcgccaccg ccgcggccgg
      901 cgcgttcttg cgctggtccc agtgcttgag cgggatgaac atggtcgagg tgttggtctt
      961 gttgccgccc cccagcaggt cgaagccgtt gatcaccatc gtgttctgca cgccgggatt
     1021 ggcctggatg atcttgccca gatcggccgc catgttgcgc gtgcgctcga gcgtagcgcc
     1081 gtcgggcagt tgtatgttgc cgaagatgta gccctggtct tcctgcggca cgaagctcga
     1141 cggcacgcgc atgaacagaa aaacgcaggt ggccaggagc agtacaaacc ccaccagcga
     1201 aatcgccggg tgccgcagga ggacttgcac cgcaccgaga aagcgctgcg tgacccagtc
     1261 gaagccgcgg ttgaacggac ggaagacgcg cgactcgtgc tcgccggcgc gcatcatgat
     1321 cgcgcacagg gcgggggtga gggtgagggc gatgaagccc gagatcacca ccgcgaagct
     1381 gagcgtcacc gcgaactgcc ggtagagcgc cccggcgatg ccgccgagga acgcgaccgg
     1441 gatgaacacc gcgcacagca ccagcacgat ggcgatcacc gcgcccgaca cctcgcgcat
     1501 cgcctcgagc gccgcctcga acggcggcat gccgcgcgtg cgcatcaggc gctcgacgtt
     1561 ctccagaact acgatggcat cgtcgactac gatgccgatg gccagcacca gcgcaaaaag
     1621 cgtcagcgtg ttgatggaga agcccaccag ccacagcccg gcgaacgccc cgatcagcga
     1681 aaccggaacg gcgatcatgg ggatcagggt ggcgcgccag gtctgcagga acaggaacac
     1741 caccgcgagc accagtagcg ccgcctcgaa gatcgtcagg ttgacctcct tgatggaggc
     1801 gtcgacgaag cgcgtggtgt cgaacgggat ggcgtagtcg acacccggcg ggaacgccgg
     1861 gcgcagctcg gtcatgcgct ttctcaccag ggcggccacc tcgagcgcgt tggagcccgg
     1921 ctgcaggtag gtcgccatgc cgatggtcgg ctggccgtcg agcgtgttgg aggtgtcgta
     1981 gttcagcgcg ccgagctcca ggcgcgcgat gtctttcagg cgcaggatgc cgccgggctg
     2041 gccgctcgcg cgcaccacga tctcgccgaa ttcctcgggc tcgaccaggc gcccggtggc
     2101 cgtgacggtg tagacgatgc tttgcccggc cggcgcgggc tccgtaccga tacgcccggc
     2161 ggcgtattgc gcattctgcg cgcgcagcgc gttggcgacg tcggccgtgg tgatacccag
     2221 gcgcgccatc ttctcggggt tgagccacac gcgcatcgag tagtcgcgcg cgccgaagat
     2281 ggtgacgtcg gcgatcccgg gcaggcgctt gagttcgtcg accaggttga tcgacgcgta
     2341 gttggagagg aacagggtgt cgcgcgcgtt gccgggcgag accagcgaca ccaccagcag
     2401 gatgtcgaag gagcgcttct gcacgatgac gccgtt
//
LOCUS       DASYJA010000078         6217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3190548, whole genome shotgun sequence.
ACCESSION   DASYJA010000078 DASYJA010000000
VERSION     DASYJA010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6217
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3190548"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VGO02_01645"
     CDS             complement(<1..256)
                     /locus_tag="VGO02_01645"
                     /inference="COORDINATES: protein motif:HMM:NF018530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-alanine antiporter"
                     /protein_id="HEV7476419.1"
                     /translation="MAWLAGVFAKYPELAVFLVVGVGYWVGGFKVRGFGLGPVTGSLM
                     VGVLLGYLVEVPVSGTAKSLLFLLFLFSIGYSVGPNFFQAM"
     gene            complement(269..1969)
                     /locus_tag="VGO02_01650"
     CDS             complement(269..1969)
                     /locus_tag="VGO02_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEV7476420.1"
                     /translation="MKAAFDWFAANPYILLFFVVGAAVAVGKVTIKGYGLGMVAAAIV
                     VGACMSAWASTYGVKMELNNFAKSLFYYLFMYGVGLRVGPSFINSLKGDGLKFTVLAV
                     FCSCLGLFGAVFFAKWWDLPAGAAGGILAGSMTMSAAIGSAEEAVRQGAFKLEAGQTF
                     EQVSGMIALSYGLTYIWGTVGIILICKYLPRWWGVDAKAAAKKYEQESGVPNVDDAGL
                     TGYRPLSLRAYRLDNTSTAGMTVRQFLDKHPEYKIVNIRRGDQNLGAGDDIVLQKGDL
                     IALGGRLEAMTSNLGLIGPEVSDAKVLNVPIDQAEVLVTNKEMVGKELKDLANTPLAG
                     TIALHHIERGGQTIPMGLHTKLERFDVLFIGGVKASIDRAGAMLGKIARPSTSTDLLT
                     LALGMILGFLIGAITFPLAGSKVGLGNAGGLLVSGVIVSSVASRLRFFGSTPNAARNI
                     LEDLGLIVFIAIVGVNAGAGLISQLSGAVAVKIFVAGFLVCSIPPVLVWAIGYHWMKI
                     NPAILMGGVAGARSHSGPAREAAKEIDSSVPWIGFPVGYAISGVLLTVFGYLAMVMSQ
                     "
     gene            complement(1966..4665)
                     /locus_tag="VGO02_01655"
     CDS             complement(1966..4665)
                     /locus_tag="VGO02_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HEV7476421.1"
                     /translation="MSKQAAAVGVGSFFSASEAREERWRELHRVAKALAAGGGQADKP
                     RQEAQALLGELAPLEDLCGYPGPRLMSLLGERLKAGDAASFARLTQRISISLLSNSYR
                     DDPEAWKSDEDDDDPHLPKILPPSIGRGQARKPYCEALIVVPGERSTWEPMREGLRKL
                     RRETDQFIIEPVVVGSFEDAVLAVLVNYNIQAVVMFDGFGYESRTPLADLRELIAAHV
                     PQAAAARDGDLAMLLSRAARALRPELDVFLSTDRDVAKMAGADEAAGIRRVFFALEEM
                     LELHLAILDGLMDRYETPYFDNLKKYAQRPIGTFHALPVARGKSIFKSNWIRDMGEFY
                     GANLFLAESSATTGGLDSLLEPTGNIKVAQDKAARAFGGDYAFFGTNGTSTSNKIVHQ
                     SLCRPGDIVLIDRDCHKSHHYGLVLAGAQPYYIDAFPLTQYSMYGSLAIKPITDALLA
                     LKAEGKLDRVRMLVLTNCTFDGHVANVERTMLECLAIKPDLVFLWDEAWFGFARFSPF
                     LRRRTAMGAAAKLRATFKDPAYKKRFQESKGALPDPDKVRVRVWETDSVHKSMSALRQ
                     GSIIVVADQDFSSVEAAFKEAYFTHTSTSPNLQIIASLDVARRQMEFEGFELVQRAIQ
                     LAIEIRREINAHPLISKYFKAATPAEMIPAEYRKSGFADYAQPGWNLAKTVAALDSDE
                     FFLDPTRITVLCGNAGYDGSQFKALLASEHDIQINKTSRNSVLVQININNTRSDLAHL
                     IKALADMSRAIDKRLAEGGAAAQAAFKERVKSLVEDVPDLPNFSRFHDAFRENPKSKT
                     NEGDMRTPYYLAYDAAAREYVKLNGKEVEDRLKNGPEMVSAKFVIPYPPGFPIMVPGQ
                     VITQETITFMRKLDVKEIHGYNASLGLELLKPQALAALKGKKS"
     gene            complement(4771..5220)
                     /locus_tag="VGO02_01660"
     CDS             complement(4771..5220)
                     /locus_tag="VGO02_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEV7476422.1"
                     /translation="MTLQVVGRGFSFEDLKVGFRFRTHRRTIAEADVAAFVNLTWLTE
                     ELFTVEDDAARAIKGRAVPGALVYSFAEGLLLPTMQDTGLAFLNATLDVKGPTLVGDT
                     IHVEAEVTEHRLTSKGDRGLVRFANKVVNQRGEVVLEYNPLRLLKRK"
     gene            complement(5217..5495)
                     /locus_tag="VGO02_01665"
     CDS             complement(5217..5495)
                     /locus_tag="VGO02_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476423.1"
                     /translation="MTRDEYVQKLKAQVDQWNAQMATWEKASAEVKNKYLQQLDEAKV
                     RRDEAIEEIKRLQSASADAWMQMMKGAETAFTAMQAAFERASKGFQKK"
     gene            complement(5538..6065)
                     /locus_tag="VGO02_01670"
     CDS             complement(5538..6065)
                     /locus_tag="VGO02_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476424.1"
                     /translation="MELQRHYPLATIQEAANLYNRYQAGTGFRAYVRVRTNLVMPMLA
                     LIVLGAVACTAGTVVFLAGARAYLMLFVLLLAPFVLIGSLLVQTYLFFGWLENRALAE
                     GLKHALEPEGPVKRWLRKNLSAKMGKFPAVPWIFAAVFLLLPLLMTASVVPKIAAVLV
                     AVYVAGIILFARFDD"
BASE COUNT         1024 a         2058 c         2074 g         1061 t
ORIGIN      
        1 tcatcgcctg gaagaagttc ggccccaccg aatagccgat gctgaatagg aacagcagga
       61 acagcagcga cttggcggtg ccggacaccg gcacctcgac gaggtagccg agcagcacgc
      121 cgaccatcag cgagccggtg accggtccga ggccgaaacc ccggaccttg aagccgccga
      181 cccagtaccc cacccccacg acgagaaata ccgccagctc cgggtatttc gcgaacactc
      241 ccgccagcca ggccacgctg gcgtgttcct attgcgacat caccatggcc aggtagccga
      301 atacggtgag cagcacgccc gagatcgcgt agccgaccgg gaagccgatc cagggcaccg
      361 agctgtcgat ctccttcgcc gcttcgcgcg caggcccgga gtgcgagcgc gcgcctgcca
      421 cgccgcccat caggatcgcc gggttgatct tcatccagtg gtagccgatc gcccagacga
      481 ggaccggcgg gatgctgcac accaggaagc cggcgacgaa gatcttcacc gccaccgcgc
      541 cgctcagctg cgagatcagc ccggcgccgg cgttgacgcc gacgattgcg atgaacacga
      601 tcagtccgag gtcctccagg atgttgcgcg cggcgttcgg cgtgctgccg aagaagcgca
      661 ggcgcgaggc gaccgacgac acgatcaccc cggataccag caggccgccg gcgttgccca
      721 ggcccacttt cgatccggcg agcgggaagg tgatggcgcc gatgaggaag ccgaggatca
      781 tgccaagcgc cagcgtcagc aggtcggtgg aggtgctcgg ccgcgcgatc ttgcccagca
      841 tcgcgcccgc gcggtcgatc gaggccttaa cgccgccgat gaacagcacg tcgaagcgct
      901 cgagcttggt gtggaggccc atcgggatgg tctggccgcc gcgctcgatg tggtgcagcg
      961 cgatggtgcc ggcgaggggc gtgttcgcca gatccttcag ctccttgccc accatctcct
     1021 tgttggtcac caggacctcc gcctggtcga tcggcacgtt gagcaccttg gcgtccgaca
     1081 cctcggggcc gatcaggccg aggttggagg tcatcgcctc gaggcggccg ccgagcgcga
     1141 tcaggtcgcc cttctgcagg acgatgtcgt cgccggcgcc gaggttctgg tcgccgcgcc
     1201 ggatgttgac gatcttgtat tccggatgct tgtcgaggaa ctgccgcacc gtcatgccgg
     1261 cagtggaagt gttgtccagc cggtaggcac gcagcgaaag cggccggtag ccggtgaggc
     1321 cggcgtcgtc gacgttcggc acgccgctct cctgctcgta cttcttcgcc gccgccttgg
     1381 cgtcgacgcc ccaccagcgc ggcaggtatt tgcagatcag gatgattccg accgtgcccc
     1441 agatgtaggt aaggccgtac gagagggcga tcatgccgct cacctgctcg aaggtctgcc
     1501 cggcttcgag cttgaacgcg ccctggcgca ccgcttcctc ggcggagccg atcgctgccg
     1561 acatggtcat ggagccggcg aggatgccgc cggccgcgcc ggcgggaaga tcccaccact
     1621 tggcgaagaa caccgcgccg aacagcccga ggcaggagca gaacaccgcc agcacggtga
     1681 acttcagccc atcgcctttc aggctgttga tgaacgaggg gccgacgcgc aggcccacgc
     1741 cgtacatgaa caggtagtag aacagcgact tggcgaagtt gttgagctcc atcttgacgc
     1801 cataggttga agcccacgcc gacatgcagg cgccgaccac gatcgccgcg gccaccatgc
     1861 ccaggccgta gcccttgatg gtgaccttgc cgacggctac cgcggcgccg acgacgaaga
     1921 acagcaggat gtacggattg gcggcgaacc agtcgaaagc ggctttcatg attttttccc
     1981 cttgagtgcg gcgagcgcct gcggcttgag cagctccagg ccgagcgacg cgttgtagcc
     2041 gtggatctcc ttcacgtcga gcttgcgcat gaaggtgatg gtttcctgcg tgatcacctg
     2101 ccccggcacc atgatcggga agcccggcgg atacgggatg acgaacttgg ccgacaccat
     2161 ctccggcccg ttcttcaggc ggtcctcgac ctccttgccg ttcagcttca cgtactcgcg
     2221 cgccgccgcg tcgtaggcaa ggtagtaagg cgtgcgcatg tcgccctcgt tggtcttgct
     2281 cttcgggttc tcgcggaagg cgtcgtggaa gcggctgaag ttgggcaggt cgggcacgtc
     2341 ctcgaccagc gacttgacgc gctccttgaa cgccgcctgc gccgccgcgc cgccctcggc
     2401 caggcgcttg tcgatggcgc gcgacatgtc ggccagggcc ttgatcaggt gcgcgaggtc
     2461 gctcctcgtg ttgttgatgt tgatctgcac cagcacgctg ttgcgcgagg tcttgttgat
     2521 ctggatgtcg tgctcgctgg cgagcagcgc cttgaactgg ctgccgtcgt agccggcgtt
     2581 gccgcaaagc accgtgatgc gcgtcgggtc gaggaagaac tcgtccgagt cgagcgcggc
     2641 gacggtcttc gccaggttcc agcccggctg ggcgtagtcg gcgaagcccg acttgcggta
     2701 ttccgccggg atcatctctg ccggtgtcgc cgccttgaag tatttcgaga tcagcggatg
     2761 cgcgttgatc tcgcggcgga tctcgatggc gagctggatc gcgcgctgca ccagctcgaa
     2821 gccctcgaat tccatctgcc ggcgcgcgac gtcgagcgag gcgatgatct gcaggttggg
     2881 cgaggtcgag gtgtgggtga agtacgcctc cttgaacgcc gcctccacgc tgctgaagtc
     2941 ctggtcggcg accacgatga tcgagccctg gcgcagcgcg gacatcgact tgtgcaccga
     3001 gtcggtttcc cagacgcgca cgcgcacctt gtcggggtcg ggcagcgcgc ctttcgattc
     3061 ctggaagcgc ttcttgtacg cgggatcctt gaacgtcgcg cgcagcttcg ccgccgcgcc
     3121 catcgccgtg cgccggcgca ggaacggcga gaagcgcgcg aagccgaacc aggcctcgtc
     3181 ccacaggaac accagatcgg gcttgatcgc caggcactcg agcatggtgc gctcgacgtt
     3241 ggcgacgtgg ccgtcgaagg tgcagttggt gagcaccagc atgcgcacgc ggtcgagctt
     3301 gccttcggcc ttgagcgcga gcagcgcgtc ggtgatcggc ttgatcgcca ggctgccgta
     3361 catcgagtac tgggtgagcg ggaaggcatc gatgtaatag ggctgcgcgc cggcgagcac
     3421 caggccgtag tggtgcgact tgtggcagtc gcggtcgatc agcacgatgt cgcccggacg
     3481 gcacagcgac tggtgcacga tcttgttcga ggtcgaggtg ccgttggtac cgaagaatgc
     3541 gtagtcgccg ccgaaggcgc gcgccgcctt gtcctgcgcc accttgatgt tgccggtagg
     3601 ctcgagcagg ctgtccaggc ctcccgtcgt ggccgaggac tcggcgagga acaggttggc
     3661 gccgtagaac tcgcccatgt cgcggatcca gttcgacttg aagatcgact tgccgcgcgc
     3721 caccggcagg gcgtggaagg tgccgatcgg gcgctgcgcg tacttcttca ggttgtcgaa
     3781 gtagggcgtc tcgtagcggt ccatcaggcc gtcgaggatg gcgaggtgca gctcgagcat
     3841 ctcctccagc gcaaagaaca cgcgccggat gcccgccgcc tcgtcggcgc cggccatctt
     3901 ggcgacgtcg cggtcggtcg acagaaagac gtcgagctcg ggccgcaggg cgcgcgccgc
     3961 gcgcgacagc agcatggcga gatcgccgtc gcgcgccgcc gcggcctgcg gcacgtgcgc
     4021 cgcgatcagc tcgcgcaggt cggccagcgg ggtgcgcgac tcgtagccga agccgtcgaa
     4081 catcaccacc gcctggatgt tgtagttgac cagcaccgcg agcaccgcgt cctcgaagct
     4141 gccgaccacc accggctcga tgatgaactg gtcggtttcg cggcgcagct tgcgcagccc
     4201 ctcgcgcatc ggctcccagg tcgagcgctc gcccggcacc acgatcagcg cctcgcagta
     4261 gggcttgcgc gcctggccgc ggccgatcga cggcggcagg atcttgggca ggtgcgggtc
     4321 gtcgtcgtcc tcgtcggact tccacgcttc cgggtcgtcg cggtagctgt tggaaagcag
     4381 cgaaatgctg atgcgctgcg tgagccgcgc gaagctcgcc gcgtcgccgg ccttcaggcg
     4441 ctcgccgagc agcgacatca gccgcgggcc ggggtagccg cacaggtcct cgaggggtgc
     4501 gagctcgccg agcagcgcct gcgcctcctg ccgcggctta tccgcctgtc cgcccccggc
     4561 agccagcgcc ttggcgacgc ggtgcagctc gcgccagcgc tcctcgcgcg cctcggaggc
     4621 cgaaaagaaa ctgcctaccc cgacggctgc ggcttgcttg ctcatgctgg atcctttgag
     4681 tggtgcgaga tacgccgacc gtcaagctag ccgcaagcag cagccgcgac tattggacct
     4741 tggtcccata gtcgcccgtc cagggtccag ctacttgcgc ttgagcagcc tcagcgggtt
     4801 gtactcgagg acaacctcgc cgcgctggtt caccaccttg ttggcgaaac gcaccaggcc
     4861 gcggtcgccc ttcgaggtga ggcgatgctc ggtcacctcg gcctcgacgt ggatcgtgtc
     4921 gccgaccagc gtcgggcctt tcacgtcgag ggttgcattg aggaaggcga gaccggtgtc
     4981 ctgcatcgtc ggcagcagca atccctcggc gaaggagtag accagcgccc ccggcacggc
     5041 gcggcccttg atggcgcgcg ctgcgtcgtc ttcgacggta aacagctcct cggtcagcca
     5101 ggtcaggttg acgaaggcgg cgacgtcggc ttccgcgatc gtgcgccggt gcgtgcgaaa
     5161 gcggaagccg accttgagat cttcgaaaga gaaaccccgg ccaacgactt gcagggtcac
     5221 ttcttttgga agcccttgct ggcgcgctcg aaggcggcct gcatcgccgt gaaggcagtc
     5281 tctgccccct tcatcatctg catccaggcg tcggccgagg cgctctgcag ccgctttatc
     5341 tcctcgatcg cctcgtcgcg gcgcaccttc gcttcgtcga gctgctgcag gtacttgttc
     5401 ttgacctccg ccgaggcctt ctcccacgtt gccatttgcg cgttccactg gtcgacctgt
     5461 gccttcagct tctgcacgta ctcatcgcgg gtcatggtgc cctcctctgg aaaggacggg
     5521 cccattctag gccaaggtca gtcgtcgaag cgcgcgaaca ggatgatgcc ggccacgtac
     5581 accgcgacca gcacggcggc gatcttcggc accacgctcg ccgtcatgag caggggcagc
     5641 agcaggaaca ccgccgcaaa gatccacggc acggccggga acttgcccat cttcgcgctg
     5701 aggttcttgc gcagccagcg cttcaccggg ccctcgggct ccagggcatg cttgagccct
     5761 tccgcgagcg cgcggttttc cagccagccg aagaacaggt aggtctgcac cagcaggctg
     5821 ccgataagga cgaaaggcgc gagcagcagc acgaagagca tcaggtaggc gcgcgcgccg
     5881 gccaggaaca ccaccgttcc cgcggtgcac gccacggcgc cgagcacgat gagcgccagc
     5941 atcggcatga ccaggttggt gcggacccgc acgtacgcgc ggaagccggt tcccgcctgg
     6001 tagcggttat agaggttcgc ggcttcctgg atcgtagcca gcggatagtg gcgctgcagt
     6061 tccatgcgcc aagcataccg aaggcccggc aggggaggct gcgactaatt ccgcactatt
     6121 ttctaccccg gggcagcggc tcgccgtctt tccggtaggc cgggggcacg tagaaattca
     6181 gtgtgcgcag cagcacgcgg ccggtgttcc tgatttc
//
LOCUS       DASYJA010000079         2423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_921346, whole genome shotgun sequence.
ACCESSION   DASYJA010000079 DASYJA010000000
VERSION     DASYJA010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921346"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VGO02_01675"
     CDS             complement(<1..637)
                     /locus_tag="VGO02_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7476425.1"
                     /translation="MFRSLIFVAVAAFALGAQAQTIKIAILGPMSFVQGENHWAGAEM
                     ARDEINKAGGINVGGKRMQVELIRADTNEIQSVPDATNAVERVITRDKVDFLIGGFRS
                     EAVLAMQEVAMDAKKIFLGVGAAHSKLGLNVEQDYNRYKYWFRVAPTKDVDLARTLFA
                     VMGSIGQQIRTDLKTDTPKVAILAEKAVWTEALVAAAQKNLPAMKMEVVGTW"
     gene            714..1157
                     /locus_tag="VGO02_01680"
     CDS             714..1157
                     /locus_tag="VGO02_01680"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEV7476426.1"
                     /translation="MPRADFVCAPRLRVRWAEVDMQKIVFNGHYLTYIDTAIAEYWRA
                     IGLPYPQGYVERYANDVYLRKATVEYLGSAHYDDELEVCCRVAKLGRTSMTFQFEIYR
                     QAQDLPLVTADLVYVNADPSTMKAAPLPDDLRARVLRYERVSPQA"
     gene            1154..1600
                     /locus_tag="VGO02_01685"
     CDS             1154..1600
                     /locus_tag="VGO02_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEV7476427.1"
                     /translation="MIWWEDFAVGERSEMGRHTFERDEIVAFARQFDPQPFHVDPAAA
                     KASPFGGLIASGWHTCAVGMRLMVDQYIGRTVSLGSPGIEDIRWLKPVRAGDTLTYSR
                     TVIESRASTSREGVGLVKHRWEAVNQSGETVLTMEGWGMYGRKPGK"
     gene            1597..2355
                     /locus_tag="VGO02_01690"
     CDS             1597..2355
                     /locus_tag="VGO02_01690"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEV7476428.1"
                     /translation="MSGRAGARIELNHKVHVLTKKEELDSVRVPGKVVIVLDIIFATT
                     TMVTALAHGAAQVLPVLDEAAARARAKDLPGGSYVLSGELYAETLAGFVHPAPLALVE
                     HGVKGKSVIYSTTNGTVAMTLAAGAARVYCGALLNAKRLVEHVAERHPRETVLIVCSG
                     SGNNFNFEDFYGAGYFVECFAARLGPAADFSDAAKAARALYRQVKVPEALLDCRIGRL
                     MASRGLAHEVEFACRLDAFPVVPALEGGSLRLVG"
BASE COUNT          381 a          822 c          791 g          429 t
ORIGIN      
        1 gccaggtgcc gacgacttcc atcttcatcg ccggcaggtt cttttgcgcc gcggctacca
       61 gggcctcggt ccataccgcc ttctcggcga ggatcgccac cttcggcgtg tcggtcttga
      121 ggtcggtgcg gatctgctgg ccgatcgacc ccattaccgc gaacagcgtg cgcgccagat
      181 ccacgtcctt tgtgggcgct acccggaacc agtacttgta gcggttgtag tcctgctcga
      241 cgttcaggcc cagcttggaa tgcgccgcgc cgacgccgag gaagatcttc ttcgcgtcca
      301 ttgccacctc ctgcattgcg agcaccgctt cggagcggaa gccgcctatc aggaagtcga
      361 ccttgtcgcg cgtgatcact cgctccacgg cgttggtcgc gtcgggcacc gactggatct
      421 cgttagtgtc ggcgcggatc agctcgacct gcatgcgctt gccgccgacg ttgatgccgc
      481 cggccttgtt gatctcgtcg cgcgccatct cggcgccggc ccagtggttc tcgccctgga
      541 cgaacgacat cgggccgaga atcgcgatct tgatggtctg cgcctgcgcg ccgagcgcga
      601 aagccgccac cgcgacaaat atcagggagc ggaacatgaa acctcctcct cgttgaatta
      661 atgtatcttg ttgtacgaat tgatgttgcc agcaaatttc ccttccgtct aggatgccga
      721 gagccgactt cgtttgtgcc ccccgcttgc gggtgcgctg ggccgaggtg gacatgcaga
      781 agatcgtgtt caacggccac tatctgacct acatcgacac ggcgatcgcc gagtactggc
      841 gcgccatcgg cctgccctat ccgcagggct acgtcgagcg ctacgcgaac gacgtctatc
      901 tgcgcaaggc gaccgtcgag tacctcggct cggcgcacta tgacgacgag ctcgaggtgt
      961 gctgccgggt cgccaagctc gggcgcacca gcatgacgtt ccagttcgag atctatcgcc
     1021 aagcgcagga cctgccgctg gtcacggcgg atctggtcta tgtgaacgcc gacccctcga
     1081 ccatgaaggc ggcacctctg cccgacgact tgcgcgcccg cgtgctgcgc tacgaacgcg
     1141 tgtcaccgca agcatgatct ggtgggagga cttcgcggtc ggcgagcgct ccgagatggg
     1201 ccgtcatacc ttcgaacgcg atgagatcgt cgcgttcgcg cggcaattcg atccgcagcc
     1261 gttccacgtc gatccggcgg cggccaaagc atcgcccttc ggcggcctga tcgcgagcgg
     1321 ctggcacacc tgcgcggtcg gcatgcgcct gatggtcgac cagtacatcg gccgcaccgt
     1381 cagcctcggc tcgccgggca tcgaggacat ccgctggctc aagccggtgc gcgccggcga
     1441 cacgctgacc tattcgcgta ccgtcatcga gtcgcgcgcc tccaccagcc gcgagggcgt
     1501 cgggctggtc aagcaccgct gggaggcggt caaccagtcg ggcgagacgg tgctcaccat
     1561 ggagggctgg ggcatgtacg gccggaagcc aggaaaatga gcggccgcgc tggcgcgcgt
     1621 atagagctca accacaaggt ccacgtcctc accaagaagg aggagctcga cagcgtgcgc
     1681 gtgccgggca aggtggtgat cgtgctcgac atcatcttcg ccaccaccac catggtgacg
     1741 gcgctcgccc acggcgcggc ccaggtgctg ccggtgctcg acgaggcggc cgcgcgcgcg
     1801 cgcgcgaagg acctgccggg cggctcgtac gtgctttccg gcgagctata tgccgagacc
     1861 ctcgccggct tcgtgcaccc ggcaccgctt gcgctcgtcg agcacggcgt gaagggcaag
     1921 agcgtgatct actcgaccac caacggtacc gtcgccatga cgctcgccgc cggcgcggcg
     1981 cgcgtctact gcggcgcgct gctcaacgcc aaacgcctgg tcgagcacgt tgccgagcgc
     2041 cacccgcggg agaccgtgct catcgtctgc tccgggtcgg gcaacaactt caacttcgag
     2101 gatttctacg gcgccggcta tttcgtcgag tgcttcgccg ctcggctggg cccggcggcg
     2161 gatttctccg acgcagccaa ggcggcgcgc gcgctctatc gccaggtgaa ggtgcccgaa
     2221 gcgctgctcg actgccgcat cggccggctg atggcctcgc gcggcctcgc tcacgaggtg
     2281 gaattcgcct gccggctcga cgccttcccg gtggtgccgg cgctggaagg cggcagcctg
     2341 cggctggtcg gctgacggcg cgctaggcgc gctcgagctc ggcgctgacc gcgaggtggt
     2401 ccgagagcat cgaccaaggc ttg
//
LOCUS       DASYJA010000080         1770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_831233, whole genome shotgun sequence.
ACCESSION   DASYJA010000080 DASYJA010000000
VERSION     DASYJA010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831233"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VGO02_01695"
     CDS             <1..813
                     /locus_tag="VGO02_01695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEV7476429.1"
                     /translation="QFLAAGVERWRDDILDLLIEVSGCEAVFERSDAEVRKLEGLEPR
                     TGFVRGNKNAARCPIIEHGLNFRVDVEQGQKTGFFLDQRENRQRVRALAAGREVLDGF
                     SYTGGFAIAALAGGATRVTGLESSADAIGIAKENLQANPLDAAKVEFRKADVFAELRK
                     LRDGGARYGLIVLDPPKFAPTAAQARNAARAYKDINLLAFKLLAPGGLLATFSCSGGV
                     PADLFQSIVAGAALDAGAEAKIIERFGAAADHPVALEFPEGEYLKGLLLLKG"
     gene            840..>1770
                     /locus_tag="VGO02_01700"
     CDS             840..>1770
                     /locus_tag="VGO02_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEV7476430.1"
                     /translation="MAEQNMVPVTILTGFLGAGKTTLLNRILKEDHGHRIAVIENEFG
                     ESGVDGEILERTEEQIVEMNNGCICCTVRGDLIRILGELKEKRDGGKLKFDRVVIETT
                     GMADPGPVAQTFFTDEEIGNYYLLDSILTVVDAKHAPKQLDEFHEAQEQVGFADRILM
                     SKTDLVSEGDVENLSKRLRRMNPRAPVKKVHFGEAKIEEILDIRGFNLNAILELDPEF
                     LTDASHEHHDEVESFVFKSDKPFDGDKLEQFLSGMIQVYGPDLLRYKGILWMKGNPRR
                     VVFQGVHMMMGGDLGKPWAKGEKKGSLLVFIGKK"
BASE COUNT          337 a          565 c          590 g          278 t
ORIGIN      
        1 cagttcctcg ccgccggggt ggagcgctgg cgtgacgaca tcctggactt gctgatcgaa
       61 gtctcgggct gcgaagctgt cttcgaacgc tccgacgccg aagtacgcaa gctcgagggc
      121 ctggagccca gaaccgggtt cgtcagggga aacaagaacg ccgcgcgctg cccgatcatc
      181 gagcatgggc tcaacttccg cgtcgacgtg gagcagggcc agaagaccgg cttcttcctc
      241 gaccagcgcg agaaccgcca gcgcgtgcgg gccctcgccg ccgggcgcga ggtgctcgac
      301 ggcttttcct acaccggcgg cttcgccatc gccgccctgg cgggaggcgc gacgcgcgtg
      361 acgggcctgg agagctcggc ggatgcgatc ggcatcgcaa aggagaacct gcaggcgaat
      421 ccgctcgatg cggcgaaggt cgagtttcgc aaggccgacg tcttcgccga gctgaggaag
      481 ctgcgcgacg gcggcgcgcg ctacggcctg atcgtgctcg acccgcccaa gttcgcgccc
      541 accgcggcgc aggcgcgcaa cgcggcgcgc gcctacaagg acatcaacct gctcgcgttc
      601 aagctgctcg cccccggcgg cctgctcgcc accttctcgt gctcgggcgg ggtgccggcg
      661 gacttgttcc agtcgatcgt cgccggcgcc gcgctcgatg cgggcgcgga agcgaaaatc
      721 atcgagcgct tcggcgcggc cgcggaccac ccggtggcgc tcgagtttcc ggagggcgag
      781 tatctgaagg gactgctgct gctgaagggg taaaatcccg cccttcgcaa ggagcatcca
      841 tggcagagca gaacatggtg ccggtgacca tcctcaccgg gtttctgggc gccggcaaga
      901 cgacgctgct caaccgcatc ctgaaggaag accacggcca ccgcatcgcc gtgatcgaga
      961 acgagttcgg cgagagcggc gtcgacggcg agatcctcga gcgcaccgag gagcagatcg
     1021 tcgagatgaa caacggctgc atctgctgca ccgtgcgcgg cgacctgatc cgcatcctcg
     1081 gcgagctgaa ggagaagcgc gacggcggca agctcaagtt cgaccgcgtg gtgatcgaga
     1141 ccaccggcat ggccgatccc ggcccggtgg cgcagacctt cttcaccgac gaggagatcg
     1201 gcaactacta cctgctcgac tcgatcctca cggtggtcga cgccaagcac gcgccgaagc
     1261 agctcgacga gttccacgag gcgcaggagc aggtcggctt cgccgaccgc attctgatgt
     1321 ccaagacgga tctggtctcc gagggtgatg ttgagaacct gtcgaaacgc ctgaggcgaa
     1381 tgaatccccg tgccccggtc aagaaggttc atttcggcga agcaaaaata gaagagatcc
     1441 tggatatccg cggcttcaac ctgaacgcga tcctcgagct cgacccggaa ttcctcactg
     1501 acgcgagcca cgagcaccac gacgaggtcg agtcgttcgt cttcaagtcc gacaagccct
     1561 tcgacggcga caagctggag caattcctct ccggcatgat ccaggtctac ggcccggact
     1621 tgctgcgcta caagggcatc ctctggatga agggcaatcc ccggcgggtc gtcttccagg
     1681 gcgtgcacat gatgatgggc ggcgatttgg gcaagccgtg ggcgaagggc gagaagaagg
     1741 gctcgctgtt ggtgttcatt ggcaagaagc
//
LOCUS       DASYJA010000081         9131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1012595, whole genome shotgun sequence.
ACCESSION   DASYJA010000081 DASYJA010000000
VERSION     DASYJA010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9131
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012595"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2423
                     /gene="glgP"
                     /locus_tag="VGO02_01705"
     CDS             <1..2423
                     /gene="glgP"
                     /locus_tag="VGO02_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEV7476431.1"
                     /translation="ALFARLHPALWDAVGHSPKAMLKRIDEQRLLDAAADPAFLDSMT
                     RVLSAFDAYHAEPPFKQNSGGFRGGDLVAYFCAEFGVHESLPIYSGGLGILAGDHCKA
                     ASDFQLPFVAMGLLYRQGYFVQTIDGEGRQRAEYSDSDFDDMPIFPVRRPDGAELRIE
                     IEFPGRTVLAKVWQARIGHVTLYLLDTGLPENSEHDRAITHRLYGGDRTTRLEQEIVL
                     GVGGVRALEAMGLKPTVWHINEGHAAFLILERLHALAAQGLGADAALEAVAASTVFTT
                     HTPVPAGHDHFADAVVLPYLKVFGDSGAAALGRPPAGGEFNMTALAIRGSRYHNGVSR
                     IHGGVSQQMLKDFWPQVAPEENPVSYVTNGVHVPTFLAPEWDAVLERYLGVGWMHRLG
                     HPGIWERIREIPDQIFWAVRQDIKARMLHMVRHRFGTQHRRSHGSESHLERLLRFADP
                     AKPNVLTIGFGRRFATYKRATLLFNDLDSLRRLLADRERPVLFLFTGKAHPADEPGQD
                     LIRTLVHMSRQEEFEGRILFLEGYDLHLARRLVSGVDVWLNNPVYPLEASGTSGMKAG
                     MNGVLNLSVLDGWWDEGYDGENGWAIKPASPELDPHRRDREEARSLYELLQDKVIPLY
                     YQRANGGAPQEWTRMAKRSMATLLPGFNASRMLGEYVSKFYLPASRQGRRYAEGDLAA
                     AKSVAAWKARVRAAWPGVSARRLDTPKGRIQFGDSMPVEVALKLNGLTPADVRVELLL
                     SRSLRGTPPIEHSHELAPAGEAHGGEQRYAIDLKPGLAGRLDYRIRIYPRNELLTHPF
                     ETGLCCWV"
     gene            complement(2383..4440)
                     /gene="glgX"
                     /locus_tag="VGO02_01710"
     CDS             complement(2383..4440)
                     /gene="glgX"
                     /locus_tag="VGO02_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEV7476432.1"
                     /translation="MEAPARPVVVPSTVWRGRPYPLGATWDGAGVNFALFSKHAERVE
                     LCLFEPKGRRELERIELRERSDFIWHCYLPEARPGQLYAYRVHGPHEPARGHRFDPAR
                     LLLDPYARLIRGKIAAGLGRCEVIDSAFSWGDDRPPRTPMRDTVLYELHVKGFTQQHP
                     EVPAQLRGTYAGLATLPVIEHLKRLGVTAVELLPVHAMADEKRLLQHGLRNYWGYNSI
                     GFFAPELRYAASGAVGEFKTMVKTLHAAGIEVILDVVFNHTAEADHHGPTLSFRGIDN
                     LIYYRVDPHDPARYLNFTGTGNSLNTAHRVVLALVMDSLRFWVEEMRVDGFRFDLAAT
                     LARGAHGGFDRNSAFLAAVRQDPLLSRVKLFAEPWDVGEGGYQLGNFPPGWSEWNDKY
                     RDAVRSYWKGDGGRVGELASRLSGSSDVFQRSGRGPTASVNFVTTHDGFTLHDLVSYE
                     HKHNEANLEENRDGSPDNRSANYGVEGPTQDAVVVSLREKQKRNLLATLLFSQGVPLL
                     LAGDEIGRTQQGNNNAYCHDSELSWLDWQLDGNERALLEFTRQLIALRNAHALFRRRT
                     WFRGRTERDPDVVWLNYAGVEMTDEEWNRSSARCLGVLLSGRGLSERDEHGVPVVDAD
                     LLLLLNSNEGDVPFPLPSGRWELLLDTGLDSPSFEKAYPLRGRSLVLLAKPSSTDPSR
                     TGE"
     gene            complement(4452..4898)
                     /locus_tag="VGO02_01715"
     CDS             complement(4452..4898)
                     /locus_tag="VGO02_01715"
                     /inference="COORDINATES: protein motif:HMM:NF027482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4864 domain-containing protein"
                     /protein_id="HEV7476433.1"
                     /translation="MKLVRIAMLALAAVVSLSALANGRVTGSDLLEIRAVINRQLDAF
                     QRDDASGAFALASPGVQQAFGTPQRFLEVVRMAYRAVYRPARVAFLEPMVLSGDVVQQ
                     VQITDRAGGVWLAYYSMQRQDDGSWRTNGCHLVQPARTIFASDEAE"
     gene            complement(4900..5526)
                     /locus_tag="VGO02_01720"
     CDS             complement(4900..5526)
                     /locus_tag="VGO02_01720"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEV7476434.1"
                     /translation="MPARNYKYKHLLAAAGLIALLGLVSEMARAPDSETDRTCVPEEV
                     EAVADPLDPEQRTLAVHLSRRFQVAAEGTGAAVFAAYRAAHEVGLDPLLVLAMISIES
                     GFNPLAESSMGAKGLMQIIPRFHLAKLEARGGEESVLDPESNVAVGARILQEYVYRTG
                     SLEAGLQQYNGASRDESAQYTQKVMAERARLERVLRRTQVTAVYAQGG"
     gene            5738..6760
                     /locus_tag="VGO02_01725"
     CDS             5738..6760
                     /locus_tag="VGO02_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEV7476435.1"
                     /translation="MKTVALVLAGGEGTRLHPLTAEHAKPALPFANGYRIVDFVLSNL
                     VNSGISTIYVLAQYKPESLMRHIAAAWSPWLDEAEGLIKVLLPRSNTLAGSFKGTADA
                     AYQYFDLLQAHKADAVAVFAADHVYRMDVRQMVAFHRERAADATVAAVAVPIAQSASF
                     GIMRTDADGRVREFCEKPRHAVSIPGNPALAYASMGNYLFRPELLGTVLAEARRRGDT
                     DFGRDVLPRLCADAGVYAYDFGHNVVPGVQDYEEHAYWRDVGTLSALAAAQQDAMGHR
                     PRFNLWNRRWPIRGEYDAALLAKVRDWRNAEPQEAEVSTAELRPAAAWQAASRDRRSD
                     ERVQPS"
     gene            complement(6690..8129)
                     /gene="glgA"
                     /locus_tag="VGO02_01730"
     CDS             complement(6690..8129)
                     /gene="glgA"
                     /locus_tag="VGO02_01730"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEV7476436.1"
                     /translation="MDPDKDFTGTRVLFAAPECAPLTKTGGLGDVAGALPSALRSLGV
                     DARVLLPRYENLNLNDKEIARATLLGRKVRILESKLPSGVPLYLLDCPDLYIRNGGPY
                     QTDEGEDWPDNALRFGVLSRAAALLGGAASPLSWRPDVVHCNDWPSALAPLYLEREAD
                     AAASLLTVHNLAFQGLFEPAVLAGLELPPSDFSLQKLEFYGRLSFLKGGLACADAVTT
                     VSPTYAAQIQTEAQGCGLDGLLRERRADLSGILNGIDTAIWDPAGDPLIAANYDATAL
                     DAKRANKAALQRRMELREDAHVPLLGMVGRLTHQKGIDLVLAAAQDLAGLPAQLVILG
                     KGERAHEHALAALAARHPGKVAVATGFNEELAHLIEAGADLFLMPSRFEPCGLNQMYS
                     QRYGTPPVAHATGGLADTIADGETGFLFDRPERGALIEAVRRALAVRREPRRWRAVQR
                     AGMARDFSWAEPARRYADLYLRLARRQPA"
     gene            complement(8214..8486)
                     /locus_tag="VGO02_01735"
     CDS             complement(8214..8486)
                     /locus_tag="VGO02_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476437.1"
                     /translation="MKPEIVERFSGVRDFYSLTKNILSICEPYGPVHSFRFVHNRGAA
                     RVACVIELESSKQTPALARALGVSGPNGAVCLEIPVDKDFPNELPA"
     gene            complement(8559..>9131)
                     /locus_tag="VGO02_01740"
     CDS             complement(8559..>9131)
                     /locus_tag="VGO02_01740"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEV7476438.1"
                     /translation="LSAPAALAVSFIMVPPGKATAGQEVKIESEASGSMDALTQGTLQ
                     GAQLLVNIVAMLVVFVALVSLANFIIAPYSLQGLLGWALAPLAWLCGVPWSEARAGGA
                     LLGTKTVLNELVAYLDLSRMSDLSERSRTLMTYALCGFANLGSLGIMIGGLGTMCPGR
                     RAEIVALGIKSIAAGTLATCLTAASVALML"
BASE COUNT         1446 a         3175 c         3112 g         1398 t
ORIGIN      
        1 gcgcgctgtt cgcccggctg catccggcgc tgtgggacgc ggtcggccac agcccgaagg
       61 cgatgctcaa gcgcatcgac gagcagcgcc tgctcgatgc ggcggccgac ccggcgttcc
      121 tcgacagcat gacgcgcgtg ctgtcggcgt tcgacgcgta ccacgccgag ccgccgttca
      181 agcagaactc gggcggcttc cgcggcggcg acctggtcgc ctacttctgc gccgaattcg
      241 gcgtgcacga gagcctgccg atctactccg gcggcctggg catcctcgcc ggcgaccact
      301 gcaaggcggc gagcgacttc cagctgccgt tcgtcgccat gggcctgctc taccgccagg
      361 gctatttcgt gcagaccatc gatggcgagg gccgccagcg cgccgagtac tcggactccg
      421 atttcgacga catgccgatc ttcccggtgc gccgcccgga cggcgccgag ctgcgcatcg
      481 agatcgagtt ccccggccgc acggtgctcg ccaaagtctg gcaggcccgc atcggccacg
      541 tcacgctgta ccttctggac accggcctgc cggagaacag cgagcacgac cgcgccatca
      601 cgcaccgcct ctacggcggc gaccgcacga cgcgcctcga gcaggagatc gtgctcggcg
      661 tcggcggcgt gcgcgcgctg gaagccatgg gcctcaagcc gacggtctgg cacatcaacg
      721 aagggcacgc cgcgttcctc atattggagc ggttgcacgc gctcgcggca caaggcctcg
      781 gcgccgacgc ggcgctcgag gcggtcgcgg cgagcactgt gttcaccacg catactccgg
      841 tacccgccgg ccacgaccat tttgccgacg ccgtggttct gccgtacctg aaagtctttg
      901 gcgactccgg cgccgccgcg ctcggccggc cgccggcggg cggcgagttc aacatgaccg
      961 cgctcgccat ccgcggctcg cgctaccaca acggcgtctc gcgcatccac ggcggcgtct
     1021 cgcagcagat gctgaaggac ttctggccgc aggtcgcgcc ggaggagaac ccggtcagct
     1081 acgtcaccaa cggcgtgcac gtgccgacct tcctcgcgcc ggaatgggac gcggtgctcg
     1141 agcgctacct cggcgtgggc tggatgcacc gcctcggcca ccccggcatc tgggagcgca
     1201 tccgcgagat ccccgaccag atcttctggg ccgtgcgcca ggacatcaag gcgcgcatgc
     1261 tgcacatggt gcgccaccgc ttcggcacgc agcaccggcg cagccacggc agcgaatcgc
     1321 acctcgagcg gctgctgcgc ttcgccgatc cggccaagcc caatgtcctg accatcggct
     1381 tcggccgccg cttcgccacc tacaagcgcg ccaccctgct gttcaacgac ctggacagcc
     1441 tgcggcgcct gctcgccgac cgcgagcgcc cggtgctgtt cctgttcacc ggcaaggcgc
     1501 acccggccga cgagcccggc caggacctga tccgcacgct ggtccacatg tcgcggcagg
     1561 aggagttcga gggccgcatc ctgttcctcg agggctacga cctgcacctc gcgcggcgcc
     1621 tggtgtccgg cgtcgacgtc tggctcaaca acccggtcta cccgctggag gcctccggca
     1681 cctccggcat gaaggcgggc atgaacgggg tcctcaacct gtcggtgctg gacggctggt
     1741 gggacgaggg ctacgacggc gagaacggct gggcgatcaa gcccgcctcg cccgagctcg
     1801 acccgcaccg ccgcgaccgc gaggaggcgc gctcgctcta cgagctgctg caggacaagg
     1861 tgatcccgct ctactatcag cgcgccaacg gcggggcgcc gcaggaatgg acgcgcatgg
     1921 cgaagcgctc gatggcaacc ctgctgccgg gcttcaacgc ctcacgcatg ctcggcgaat
     1981 acgtctcgaa gttctatctg cccgcatcgc gccaggggcg gcgctacgcc gagggcgatc
     2041 tggccgcggc gaagtcggtc gcggcctgga aggcgcgcgt gcgcgccgcc tggcccgggg
     2101 tctccgcgcg ccgcctcgat acgccgaaag gccgcatcca gttcggcgac agcatgccgg
     2161 tggaagtggc gctgaagctg aacgggctca ctcccgccga cgtgcgcgtc gagctgctgc
     2221 tgtcgcgctc gctgcgcggc acgccgccga tcgagcacag ccacgagctg gcgccggcgg
     2281 gcgaggcgca cggcggcgag cagcgctatg cgatcgatct caagcccggc ctcgccggcc
     2341 ggctcgacta ccgcatccgc atctacccgc gcaacgagtt gctcactcac ccgttcgaga
     2401 cgggtctgtg ctgctgggtt tagcgagcag caccagtgaa cgcccccgca gggggtaagc
     2461 tttttcgaat gatgggctgt cgaggccggt atccagcagc aattcccagc gaccggaggg
     2521 cagcgggaac ggcacgtctc cttcgtttga attgagcagt aggagaagat cggcgtccac
     2581 gaccggcacg ccgtgctcgt cgcgctcgct cagcccgcgc ccggagagca gcacgccgag
     2641 gcagcgcgcg ctcgagcggt tccattcctc gtcggtcatt tccacgcccg cgtagttcag
     2701 ccacaccaca tcggggtcgc gctcggtgcg accgcgaaac caggtgcggc ggcggaacag
     2761 cgcatgcgca ttgcgcagcg cgatcagctg ccgcgtgaac tcgagcagcg cgcgctcgtt
     2821 gccgtcgagc tgccagtcga gccacgagag ctcactgtca tggcagtagg cgttgttgtt
     2881 gccctgctgc gtgcggccga tctcgtcgcc ggccagcagc agcggcacgc cctgcgagaa
     2941 aagcagggtg gccagaagat ttctcttttg tttttctctc agggaaacca caacggcgtc
     3001 ttgcgtgggc ccttccaccc cgtagttggc gctgcggttg tcgggcgagc cgtcgcggtt
     3061 ttcctcgagg ttggcctcgt tgtgcttgtg ctcgtagctc accaggtcat gcagcgtgaa
     3121 accgtcgtgc gtggtgacga agttgacgct cgccgtcggg ccgcggccgc tgcgctggaa
     3181 gacatcgctc gagccggaca ggcgcgaggc gagctcgccg acgcggccgc catcgccctt
     3241 ccagtaggag cgcaccgcgt cgcggtactt gtcgttccac tccgaccagc cgggtgggaa
     3301 gttgcccagc tggtagccgc cctcgcccac gtcccagggc tcggcgaaca gcttgacgcg
     3361 cgacagcagc ggatcctggc gcaccgccgc gagaaaggcg ctgttgcggt cgaaaccgcc
     3421 gtgcgcgccg cgcgccagcg tggcggcgag gtcgaagcgg aagccgtcga cgcgcatctc
     3481 ctcgacccag aagcgcaggc tgtccatgac cagcgcgagc accacgcggt gcgccgtgtt
     3541 gaggctgttg ccggtgccgg tgaagttgag gtagcgcgcc gggtcgtgcg ggtcgacgcg
     3601 gtagtagatg agattgtcga tgccgcggaa gctgagcgtc ggcccgtgat gatcggcctc
     3661 ggccgtgtgg ttgaagacca cgtcgaggat cacctcgatg cccgccgcgt gcagcgtctt
     3721 taccatggtc ttgaactccc ccacggcgcc gcttgccgcg tagcgcagct ccggcgcgaa
     3781 aaagccgatg ctgttgtagc cccagtagtt gcgcagcccg tgctgcagga ggcgcttctc
     3841 gtcggccatg gcgtgcaccg gcagcagctc gaccgcggtg acgccgaggc gcttgaggtg
     3901 ctcgatcacc ggcagggttg cgagcccggc ataagtgccg cgcagctgcg cgggcacttc
     3961 cgggtgctgc tgcgtaaagc ccttcacgtg cagctcgtag agcacggtat cgcgcatcgg
     4021 cgtgcgcggc ggccggtcgt cgccccagct gaaagcgctg tcgatcacct cgcagcggcc
     4081 cagtccggcg gcgattttgc cgcggatcag gcgcgcgtaa gggtcgagca gcagccgcgc
     4141 gggatcgaag cggtggccgc gcgccggctc gtgcgggccg tgcacgcggt aggcatagag
     4201 ctgcccgggg cgagcctcgg gcaggtagca gtgccagatg aaatcgctgc gctcgcgcag
     4261 ctcgatacgc tcaagctcgc gccggccctt cggctcgaac aggcacagct cgacgcgctc
     4321 ggcgtgcttc gagaacaggg cgaaattgac cccggcgccg tcccaggtcg cccccagcgg
     4381 ataggggcgt ccgcgccaga cggtgctcgg tacgacgaca ggccgggccg gtgcctccac
     4441 tcccgccgag cttactcggc ctcgtcggaa gcgaaaatgg ttctcgccgg ctgcaccagg
     4501 tggcagccgt tggtgcgcca gctgccgtcg tcctggcgct gcatggagta gtacgcgagc
     4561 cagacgccgc cggcgcggtc ggtgatctgc acctgctgca cgacgtcccc cgagagcacc
     4621 atcggctcga ggaacgctac ccgcgccggc cggtatacgg cgcggtatgc catgcgcacc
     4681 acctcgagga agcgctgcgg cgtgccgaag gcctgctgca cgccgggcga ggcaagcgcg
     4741 aaggcgccgg acgcgtcgtc gcgctggaac gcgtcgagct gccggttgat cacggcgcgg
     4801 atctcgagca ggtcggagcc ggtgacccgg ccgttggcga gcgccgacag gctcaccact
     4861 gccgccagcg cgagcattgc gatccggacc agcttcatct cagcctccct gagcgtagac
     4921 cgcggtcacc tgtgtgcgtc gcagcacccg ctccaggcgc gctctttcgg ccatcacctt
     4981 ctgcgtgtat tgcgccgatt cgtcgcgcga cgcgccgttg tactgctgca acccggcctc
     5041 caggctgccg gtgcggtaga cgtattcctg caggatgcgg gcaccgaccg cgacgttgct
     5101 ttccgggtcg agcaccgatt cttccccgcc gcgcgcctcg agcttggcca agtggaaccg
     5161 ggggatgatc tgcatcaacc ccttggcgcc catcgagctc tcggcgagcg ggttgaagcc
     5221 ggattcgatc gagatcatcg ccagcaccag cagcgggtcg agcccgactt cgtgcgccgc
     5281 ccggtaggcg gcgaagaccg ccgccccggt cccctcggcg gcgacctgga agcggcgcga
     5341 cagatgcacc gcgagtgttc tctgttccgg atccagcggg tcggcaaccg cctcgacttc
     5401 ctccggcacg caggtgcggt ctgtttccga gtcgggggcc cgcgccattt ccgagacgag
     5461 gccgaggagc gcgatcagcc cggcggcggc cagcaggtgc ttgtacttgt agttgcgcgc
     5521 gggcatgacc ggaaaagatc gtagtggcag cccggcgcgg gcggtatcgc cgccctatga
     5581 atttgctgtc gcgcatcggc tacgcgcggt cggcccgatc gcgacacgcg aaacccatcc
     5641 cgggcgatag tcgctgagac aacgactacc tatcatccaa cgcgtcactc gacgaactag
     5701 aaacctagaa accacttcaa gaaaggcgcc ccggaccatg aaaaccgttg ctctcgttct
     5761 cgccggcggt gaaggcaccc gactgcaccc cctgaccgcc gagcacgcga agccggccct
     5821 gccgttcgcc aacggctaca ggatcgtcga cttcgtgctg agcaacctgg tgaattcggg
     5881 aatctccacc atctacgtgc tggcgcagta caagcccgaa tcgctcatgc gccacatcgc
     5941 cgccgcctgg tcgccgtggc tcgacgaggc cgaagggttg atcaaggtgc tgctgccgcg
     6001 ctccaacacg ctcgccggca gcttcaaggg tacggccgac gccgcctacc agtacttcga
     6061 cctgctgcag gcgcacaagg ccgatgccgt ggcggtcttc gccgccgacc atgtgtaccg
     6121 catggatgtc aggcagatgg tggcgttcca ccgcgagcgc gcggccgacg ccacggttgc
     6181 ggccgtcgcg gtgccgatcg cgcagtccgc ctccttcggc atcatgcgca ccgacgccga
     6241 tggccgcgtg cgtgagttct gcgagaagcc gcgccatgcc gtctcgatcc ccggcaatcc
     6301 ggcgctggcc tacgcctcga tgggcaacta cctgttccgg cccgagctcc tgggcacggt
     6361 gctggccgag gcgcgccgcc gcggcgatac cgacttcggt cgcgacgtgc tgccgcgcct
     6421 gtgcgcggac gcgggcgtct acgcctacga tttcggccac aacgtggtgc ccggcgtgca
     6481 ggactacgaa gagcatgcct actggcgcga cgtcggcacg ttgagcgcgc tcgccgccgc
     6541 gcagcaggac gcgatgggcc accgcccgcg cttcaacctg tggaaccggc gctggccgat
     6601 ccgcggcgag tacgacgcgg cgctgctcgc caaggtgcgc gactggcgca atgccgagcc
     6661 gcaggaggcc gaggtctcga ccgccgagct caggccggct gccgcctggc aagccgcaag
     6721 tagagatcgg cgtagcgacg agcgggttca gcccagctga agtcgcgcgc catgccggcg
     6781 cgctgaacgg cgcgccagcg ccgcggctcg cggcgcaccg ccagcgcccg gcgcaccgcc
     6841 tcgatcaggg cgccgcgctc cgggcgatcg aacaggaacc cggtttcgcc gtcggcgatg
     6901 gtatcggcca ggccccccgt ggcgtgcgcc acgggcgggg tgccgtacct ctggctgtac
     6961 atctggttca gcccgcaggg ctcgaagcgc gagggcatga ggaacaggtc cgctcccgcc
     7021 tcgatcaggt gcgcgagctc ctcgttgaag cccgtggcga cggccacctt cccgggatgg
     7081 cgcgcggcga gcgcggcgag cgcatgctcg tgggcgcgct cgcccttgcc gaggatgaca
     7141 agctgcgccg gcaggccggc gaggtcctgg gcggcggcca gcacgaggtc gattcccttc
     7201 tggtgcgtga gccggcccac catgccgagg agcggcacgt gcgcgtcctc gcgcagctcc
     7261 atgcgccgct gcagcgccgc cttgttggcg cgcttggcgt cgagcgcggt cgcatcataa
     7321 ttcgccgcga tcagcggatc acccgccggg tcccagatcg ccgtgtcgat gccgttcagg
     7381 attccgctca ggtcggcgcg ccgctcgcgc agcaggccgt ccagcccgca gccctgcgcc
     7441 tcggtctgga tctgagcggc gtaggtcggg ctgacggtgg tcacggcgtc ggcgcaggcc
     7501 aggccgccct tgaggaagga cagccggccg tagaactcga gcttctgcaa tgagaaatcg
     7561 ctcggcggca gctcgagccc ggcgagcacc gccggctcga acaggccctg gaacgcgagg
     7621 ttgtgcaccg tgagcaggct cgccgcggcg tccgcctcgc gctcgaggta gagcggcgcg
     7681 agggcgctcg gccagtcgtt gcagtgcacg acgtccgggc gccaggacaa gggggaagcg
     7741 gccccgccaa gcagcgcagc ggcgcgcgac agcactccaa agcgcagggc gttatcgggc
     7801 cagtcctcgc cctcgtccgt ctggtagggc ccgccgttcc ttatatagag gtccgggcag
     7861 tccagcagat agagcggcac cccggacggc agcttcgact ccaggatgcg gactttcctt
     7921 cccagcaggg tcgctctggc aatttctttg tcattcagat tcaaattctc atatcgcggc
     7981 agcagtaccc gcgcatccac gcccagcgag cgcagcgcag agggcagcgc gcccgcgacg
     8041 tcgcccaagc cgccggtctt ggtgagcggc gcgcattcgg gcgcggcgaa cagcacccgg
     8101 gtgccggtga aatccttatc ggggtccaca aaaaaaagac cccggtaggg ggaccggggt
     8161 cggattacat acgcacgaag ggagggatgt gcgcatgcaa gggggaaaaa ttcttaagcg
     8221 ggcagctcgt tcgggaagtc cttgtcgacg gggatctcca ggcataccgc gccgttcggc
     8281 ccgctcacgc cgagcgcgcg ggcgagcgcc ggggtctgct tggacgactc gagctcgatg
     8341 acgcatgcga cgcgcgcggc gccgcggttg tggacgaagc ggaacgagtg caccggcccg
     8401 tagggctcgc aaatcgacag gatgttcttg gtcagggaat agaagtcgcg cactccgctg
     8461 aaccgctcga cgatttccgg cttcatttgc aaggcatgct agggcccgga cacggcgccg
     8521 ggtatcgcaa gactcgtgat actgctgtag tacggcgact acagcatcag cgcgacactc
     8581 gcggcggtca ggcaggtggc cagcgtgccg gcggctatcg acttgattcc caaggccacg
     8641 atctccgcgc gccggcccgg gcacatggtc ccgaggccgc cgatcatgat gcccaggctg
     8701 cccaggtttg cgaatccgca cagcgcgtag gtcatgagcg tgcggctgcg ctccgataaa
     8761 tcggacattc gggaaagatc aaggtaggcg acgagctcat tgagcaccgt tttcgtgcca
     8821 agcagcgcgc cgccggcgcg cgcttcgctc cacggcacgc cgcacagcca ggcgagcggc
     8881 gcgagcgccc agccaagcag gccctgcagg gaatacgggg cgatgatgaa gttggcgagc
     8941 gagaccagcg cgacgaacac caccagcatg gcgacgatat tgaccagcag ctgcgcgccc
     9001 tgcagcgtgc cctgggtcag ggcatccatg ctgcccgatg cctccgattc aatctttacc
     9061 tcttgccctg cggtcgcttt tcctggcggg accatgatga acgagacagc aagcgccgcg
     9121 ggcgcagaaa g
//
LOCUS       DASYJA010000082         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2101412, whole genome shotgun sequence.
ACCESSION   DASYJA010000082 DASYJA010000000
VERSION     DASYJA010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2101412"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VGO02_01745"
     CDS             complement(<1..512)
                     /locus_tag="VGO02_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEV7476439.1"
                     /translation="MPDIVIHVIACAAYVGLAWHFWNTRWRRQASGGLRGWERAAILA
                     PLALHGWLLYDGIFAAPELRFGFAQALSVMTWLAVLAYWSESLLHNLDGMEPLVLLAA
                     AGSVTLPAFFPGLASSSVHSQSGEFKLHLALAMAAYGLFVIALLHAVLMLVVERRLHA
                     KKAGFAGNLPP"
     gene            532..>1556
                     /locus_tag="VGO02_01750"
     CDS             532..>1556
                     /locus_tag="VGO02_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048500 - signal recognition particle
                     [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEV7476440.1"
                     /translation="MLDTLTGRLAKIVKTIRGEARLTEANIQDALREVRVALLEADVA
                     LPVVKHFTEAVRAKALGEEVAGSLTPGQALVGVVHRELAALMGEANAGLELAVQPPAV
                     ILLAGLQGSGKTTTSGKLARFLKESKKKVALVSADVYRPAAIEQLKTVAAQVGADFIS
                     SSPSEKPLEIAKRAVEYARTHYMDVLIVDTAGRLAIDEQMMQEIAALHAELKPAETLF
                     VVDAMQGQDAVNVARVFSERLPLTGIILTKLDGDSRGGAALSVRHVTGKPIKFAGTGE
                     KLDGLEPFHPERMAARVLGMGDIVSLVEEAHARIDLEEAKRVAEKVKKGKGFDLEDFK
                     QQIAQMK"
BASE COUNT          289 a          506 c          555 g          206 t
ORIGIN      
        1 ggcggcaggt tgcccgcaaa gccggctttc ttcgcgtgca ggcgccgctc gacgaccagc
       61 atgagcacgg cgtgcagcag ggcgatgacg aacaggccgt aggcggccat cgccagcgcg
      121 aggtgcagct tgaattcacc cgactgcgaa tgcacgctgc tcgaggcaag gcccgggaag
      181 aaggccggca gcgtgacgct gcccgcggcg gcgagcagca ccaggggctc catgccgtcg
      241 aggttgtgga gcaggctctc gctccagtag gcgagcaccg cgagccaggt catgaccgag
      301 agcgcctggg cgaagccgaa gcgcagctcg ggcgcggcga agatgccgtc atagaggagc
      361 cacccgtgca gcgcgagcgg cgcgaggatc gcggcccgct cccacccgcg cagcccgccg
      421 ctcgcctggc ggcgccaacg cgtgttccag aagtgccacg ccagccccac gtacgcggcg
      481 cacgcgatta cgtgaattac aatgtcgggc acctcttcag tttatcaccc catgctggat
      541 accctgaccg gacgcctcgc caagatcgtc aagacgatcc gcggcgaagc gcgcctgacc
      601 gaagcgaaca tccaggacgc gttgcgcgaa gtgcgcgtgg cgctgctcga ggcagacgtc
      661 gcgctgccgg tggtgaagca cttcaccgag gcggtgcgcg ccaaggcgct cggcgaggag
      721 gtggccggca gcctgacgcc ggggcaggcg ctggtcggcg tggtgcaccg cgagctcgcc
      781 gcgctgatgg gcgaggcgaa cgccggcctc gagctcgccg tgcagccgcc ggcggtgatc
      841 ctgctggccg gcctgcaggg ctcgggcaag accaccacca gcggcaagct ggcccgcttc
      901 ctgaaggaga gcaagaaaaa agtggcgctg gtgtcggccg acgtctaccg gccggcggcg
      961 atcgagcaac tgaagacggt ggctgcccag gttggagcgg actttatttc ttcgtctcct
     1021 tctgaaaaac ccctggaaat cgcgaaaagg gcggtcgagt acgcgcgcac gcattacatg
     1081 gacgtgctga tcgtcgacac cgcagggcgc ctggcgatcg acgagcagat gatgcaggag
     1141 atcgcggcgc tgcacgcgga gctgaagccc gccgaaacgc tgttcgtggt cgacgccatg
     1201 cagggccagg acgcagtcaa cgtggcgcgc gtcttcagcg agcggctgcc gctcaccggc
     1261 atcatcctca ccaagctgga cggcgactcg cgtggcggcg ctgcgctctc cgtgcgccac
     1321 gtcaccggca agccgatcaa gttcgccggc accggcgaga agctcgacgg cctggagccg
     1381 ttccacccgg agcgcatggc ggcccgcgtg ctcggcatgg gcgacatcgt gtcgctggtc
     1441 gaggaagcgc acgcccgcat cgacctggag gaggcgaagc gcgtcgcgga gaaggtgaag
     1501 aagggcaagg gcttcgacct agaggacttc aagcagcaga tcgcgcagat gaagaa
//
LOCUS       DASYJA010000083         4918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2283436, whole genome shotgun sequence.
ACCESSION   DASYJA010000083 DASYJA010000000
VERSION     DASYJA010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2283436"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="VGO02_01755"
     CDS             <1..1179
                     /locus_tag="VGO02_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV7476441.1"
                     /translation="RWIVEEVQSLAREGVPLSHIALLYRSNAQSRVVEHALFSRGVAY
                     RVYGGLRFFERAEIKHALAYLRLVAMPDDDNAFLRVVNFPARGIGARTVEQIQDAAKA
                     GGVSLMKSVDGNSKAAAFKKLIEDLKTETQNLPLPEMVEHVVARSGLIEHYKTEREGA
                     DRIENLAELTNAAAAFTDEERQAESGEAVDPLTAFLTHAALEAGEHQAGEGQEALQMM
                     TVHSAKGLEFDVVFLSGLEEGLFPHEQSVSGTNATHDGLEEERRLAYVAITRARNRLY
                     LSHAQTRMLHGQTRYNIPSRFLEEIPEALLKWLTPRFSKQKAFTPDFSRTKETFPLKK
                     PSRDVGGFRIGQNVTHPKFGSGVIIDAEGQGTDARVQVNFGGQGVKWLAVAVAKLQAA
                     "
     gene            complement(1182..1526)
                     /locus_tag="VGO02_01760"
     CDS             complement(1182..1526)
                     /locus_tag="VGO02_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476442.1"
                     /translation="MDFCLFARRAALPLCAALLAAACGEKSARISVACQVTPNKGLNC
                     KMNNLGPDLGSVCFDAVVKCSMGDKVAKICSAPIPPKMAETRAVAALEPPIGLRETCY
                     HAEIQNTKVRPE"
     gene            complement(1547..2638)
                     /locus_tag="VGO02_01765"
     CDS             complement(1547..2638)
                     /locus_tag="VGO02_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionic transporter y4hA"
                     /protein_id="HEV7476443.1"
                     /translation="MHSLWRREWSLLLSYGTAAVFYVFHQQMLGRMESPLWAALAFAW
                     LFAVILQSAFAVVRHSESLASLVGEPFGTLILTFAITAIEVMMIAAVMLTGPPVSFLA
                     RDTMFAVVMIVFNGMAGVALLLGGLRYHEQTYNLQGANAFFAVIVPLAVIGLVLPNFT
                     VSGQGPTLSTMHEIFLIFMSLALYGVFLAIQTLSHRKYFLGPDESTGHAEHEVRSAQW
                     HGTLLVANIVPIILLSKQIALPIEFGVNLFNAPEAVVGFLVAVLVLSPESLAAVRAAL
                     ANQIQRSINLLLGSVLASISLTIPAVLAIGLLTERTVVLGLAPVDTIMLLLTLGLSTL
                     TFASGRTNVLLGAVHLLLFLAYLMLIFER"
     gene            2719..3690
                     /gene="dctP"
                     /locus_tag="VGO02_01770"
     CDS             2719..3690
                     /gene="dctP"
                     /locus_tag="VGO02_01770"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEV7476444.1"
                     /translation="MKRVLLGLICLLVAAPALAQKVVLRFSTAAPPADFLSRSMQQFK
                     ADLEKAAPEFDVQLHYASSLFRQGTEVPAMQRGNLEMSTMTTFEVAQQVPELGFFNRA
                     YLFRDYEQAYKALSGPVGKRYGELVAAKMDLLILAPTYLGTRQVALRAVREVKGPADL
                     AGLKLRMPGGPDWLLLAQSIGATPTPMGMPEVYISLQTGTIDGQENPPTIFNAAKFHE
                     VSKQMVLTSHLVQPIFYAIAKPYFDKLSPEQQRKVREAAVRGAKWNDDNRLTDEKTVI
                     DGLAAKGLTVSRPDLAPFRANADKVYAGAEAAKAWDRKLMEEAMAVR"
     gene            3690..4124
                     /locus_tag="VGO02_01775"
     CDS             3690..4124
                     /locus_tag="VGO02_01775"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEV7476445.1"
                     /translation="MRRAAAAVSALLVAAVFVAILLGVARRYLLGTPLVWIDELSTVL
                     FVWAIFWTGAFVLGIREHVSFDLVHEALPPRVAQILWRVVCALMAVALIAAVPKLWNF
                     IAFLWRERTPALQWRLDFVYACFALFIAAGALRFGWEAVRRR"
     gene            4121..>4918
                     /locus_tag="VGO02_01780"
     CDS             4121..>4918
                     /locus_tag="VGO02_01780"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEV7476446.1"
                     /translation="MSGPFLLLVAVFFVLTVARVPIAFAMLVAALAYLAAAGRDLGLV
                     ADQVMNTLMNNYLLVAIPMFLLAANLMNAGSISDRLFAACNVLVGRIRGGLAQVDVLV
                     SVLFASMSGSAVADAAGPGLVTIRAMEKAGYPRGFAAAIVAASSVLGPIIPPSIPMVL
                     YALIANVSVAALFLGGVIPGLLIAAAMMVVVAITAKRRNFPVEPPVPAAERPRVLARA
                     LLPLGMPAVLLGGIYGGVFTPTEAAAVAALYALVLAGVVYRELSAGSV"
BASE COUNT          798 a         1668 c         1656 g          796 t
ORIGIN      
        1 cgctggatcg tcgaggaggt gcagtcgctg gcgcgcgagg gcgtgccgct gtcgcacatc
       61 gcgctgctct accgctcgaa cgcgcagtcg cgcgtcgtcg agcacgccct cttctcgcgc
      121 ggcgtcgcct accgcgtcta cggcggcctg cgcttcttcg agcgcgccga gatcaagcac
      181 gcgctggcct acctgcgcct ggtcgccatg cccgacgacg acaacgcctt cctgcgcgtg
      241 gtgaacttcc cggcgcgcgg catcggcgcc cgcacggtcg agcagatcca ggacgccgcg
      301 aaagcgggcg gggtttctct catgaaatcg gtggacggga attccaaggc cgccgctttc
      361 aagaaattga ttgaagatct aaagacggaa acgcaaaacc tgccgctgcc cgagatggtc
      421 gagcatgtcg tcgcgcgcag cggcctcatc gagcactaca agaccgagcg cgaaggcgcc
      481 gaccgcatcg agaacctggc cgagctgacc aacgccgccg ccgcgtttac cgacgaggag
      541 cgccaggccg agtcgggcga ggcggtcgac ccgctcaccg ccttccttac ccacgccgcg
      601 ctggaggccg gcgagcacca ggcgggcgaa ggccaggagg cgctgcagat gatgacggtg
      661 cactcggcga agggcctgga gttcgacgtc gtgttcctca gcggcctcga ggagggattg
      721 tttccgcacg agcagagcgt aagcgggaca aacgccacac acgacggcct ggaagaggaa
      781 cgccgtcttg cctacgtcgc cattacccgt gcgcgcaacc gcctgtacct gtcccacgcg
      841 cagacgcgca tgctgcacgg ccagacgcgc tacaacatcc cctcgcgctt cctcgaggag
      901 attcccgagg cgctgctcaa gtggctgacg ccgcgcttct cgaagcagaa ggctttcacc
      961 cctgatttct cacgcaccaa ggaaaccttt ccattgaaaa aaccctcgcg ggacgtcggc
     1021 ggctttcgca tcggacaaaa cgtgacgcat ccgaagttcg gctcaggcgt gatcatcgac
     1081 gccgaaggcc agggcacgga cgcgcgcgtg caggtcaatt tcggcggcca gggcgtgaag
     1141 tggctcgccg tcgcggtggc caagctgcag gcggcgtagg gctactccgg ccggaccttc
     1201 gtattctgga tctcggcgtg gtagcaggtt tcccgcagcc cgatcggcgg ctcgagcgcc
     1261 gccaccgccc tggtctcggc cattttcggc gggatgggcg cggagcagat cttcgccacc
     1321 ttgtcgccca tcgagcattt caccaccgcg tcgaagcaga ccgatcccag gtccgggccc
     1381 aggttgttca ttttgcagtt gagccccttg ttcggcgtga cctggcaggc cacgctgatc
     1441 ctggcgctct tctcgccgca ggcggccgcc agcagcgccg cgcagagcgg cagcgccgcg
     1501 cgccgcgcga acaggcaaaa gtccattggc gttctcccct tccccgtcaa cgctcgaaga
     1561 ttagcatcag gtaggcgagg aagagcagca ggtgcacggc gccgagcagc acgttggtgc
     1621 ggccgctcgc aaaagtgagc gtgcttagcc cgagcgtcag caggagcatg atcgtgtcca
     1681 ctggcgccag gccgaggacc accgtccgct cggtgagcag gccgatcgcc agcaccgccg
     1741 gaatggtcag gctgatgctc gccagcacgg agccgagcag caggttgatc gagcgctgaa
     1801 tctggttggc gagcgcggcg cgcacggcgg cgagcgattc cggcgagagg accagcaccg
     1861 ccaccaggaa gccgaccacc gcctcgggcg cattgaacag gttgacgccg aactcgatcg
     1921 gcagcgcgat ctgcttcgag agcaggatga ttggcacgat gttggcgacc agcagcgtgc
     1981 cgtgccactg cgccgagcgg acctcgtgct ccgcgtgccc ggtggattcg tcggggccga
     2041 ggaagtattt gcggtggctc agtgtctgga tggcgaggaa cacgccatac agggcgagcg
     2101 acatgaagat caggaagatc tcatgcatcg tcgagagcgt cggcccttgc ccggagacgg
     2161 tgaaattggg cagcaccaga ccgatgacgg cgagcggcac gattaccgcg aagaacgcgt
     2221 tcgcgccctg caggttatag gtctgctcgt gatagcgcag cccgccgagc agcagcgcga
     2281 cgcccgccat gccgttgaac acgatcatca ccaccgcgaa catggtgtcg cgcgcgagga
     2341 acgaaaccgg cgggccggtg agcatcacgg ctgcgatcat catcacctcg atggcggtga
     2401 tggcgaaggt caggatcagc gtgccgaacg gctcgccgac caggctcgcc aggctctccg
     2461 agtgccgcac gacggcaaac gccgactgca ggatcaccgc gaacagccag gcgaaggcga
     2521 gcgccgccca cagcgggctc tccatccgcc cgagcatctg ctgatggaag acgtagaaga
     2581 ccgctgcggt gccgtagctg aggagcaggg accattcgcg gcgccagaga ctgtgcacgc
     2641 gccgattgtc gcctagtttt tgactccccc aatttatcta agtagcctga gcctgtccgt
     2701 ggaaacaggg gagcccgcat gaagcgcgtc ttgttgggat tgatctgtct gctggtggcc
     2761 gcaccggcgc tcgcgcagaa ggtggtgctg cgcttctcca ccgccgcgcc gccggccgac
     2821 ttcctgtcgc gctcgatgca gcagttcaaa gccgacctgg aaaaggcggc gccggagttc
     2881 gacgtgcagc tgcactacgc gagctcgctg ttccgccagg gcaccgaggt gccggcgatg
     2941 cagcgcggca acctcgagat gagcaccatg accaccttcg aggtggcgca gcaggtgccc
     3001 gagctcggct tcttcaaccg cgcctacctg ttccgcgact acgagcaggc ctacaaggcg
     3061 ctgagcgggc cggtcggcaa gcgctacggg gagctggtgg cggcgaagat ggacctgctg
     3121 atcctggcgc cgacttacct gggaacgcgc caagtggcgc tgcgcgcggt gcgcgaggtg
     3181 aaagggccgg ccgacctcgc cgggctcaag ctgcgcatgc cgggcggtcc cgactggctg
     3241 ctgctagccc agtcgatcgg cgccacgccg acccccatgg gcatgcccga ggtgtacatc
     3301 tcgctgcaga ccggcaccat cgacggccag gagaacccgc cgaccatctt caacgccgcc
     3361 aagttccacg aggtttcgaa gcagatggtg ctcacctcgc acctggtgca gccgatcttc
     3421 tacgccatcg ccaagcccta cttcgacaag ctctcgcccg agcagcagcg caaagtgcgc
     3481 gaggctgcgg tgcgcggcgc gaagtggaac gacgacaacc gcctgaccga cgaaaagacg
     3541 gtgatcgacg gcctcgccgc gaagggcctc accgtttcgc ggcccgatct cgcgccgttt
     3601 cgcgccaacg cggacaaggt ctacgccggc gccgaagcgg ccaaggcctg ggaccgcaag
     3661 ctgatggaag aagcgatggc ggtgcgctga tgcgccgggc cgcggcggcg gtctcggccc
     3721 tgctggtcgc cgcggtcttc gtcgccatcc tcctcggggt cgcgcgccgc tacttgttgg
     3781 gcacgccgct cgtctggatc gacgagctgt ccaccgtgct gttcgtgtgg gccatcttct
     3841 ggaccggtgc tttcgtcctc ggcatccgcg agcacgtcag cttcgacctg gtgcacgagg
     3901 cgctgccgcc gcgcgtggcg cagatcctgt ggcgcgtggt atgcgcgctg atggccgtgg
     3961 cgctcatcgc tgcggtgccc aagctgtgga acttcatcgc cttcctctgg cgcgagcgca
     4021 cgcccgcgct gcaatggcgc ctcgacttcg tctacgcctg cttcgcgctg ttcatcgcgg
     4081 ccggcgcgct gcgcttcggc tgggaggcgg tgcggcgcag gtgagcggcc cctttctcct
     4141 gctggtggcg gtttttttcg tcctcaccgt ggcgcgggtg ccgatcgcct tcgccatgct
     4201 ggtcgccgcg ctcgcctatc tcgcggcggc cggccgcgac ctcggcctgg tcgccgacca
     4261 ggtgatgaac acgctgatga acaactacct gctggtggcg atcccgatgt tcctgctcgc
     4321 cgccaacctg atgaacgcgg gctcgatcag cgaccgcctg ttcgccgcct gcaatgtcct
     4381 ggtcggccgc atccgcggcg gcctggcgca ggtcgacgtg ctggtgagcg tcctcttcgc
     4441 cagcatgagc ggcagcgcgg tggcggacgc cgccgggccg ggcctggtca ccatccgcgc
     4501 gatggagaag gccggctacc cgcgcggctt cgccgcggcg atcgtcgccg cctcgtcggt
     4561 gctcggcccg atcatcccgc cgtcgatccc gatggtgctg tacgcgctca tcgccaacgt
     4621 ctccgtggcg gccctgttcc tcggcggcgt gatccccgga ttgctgatcg ccgccgccat
     4681 gatggtcgtg gtggcgatca cggcgaaacg gcgcaatttc ccggtggagc cgccggtccc
     4741 cgcggccgag cggccgcgcg tcctggcgcg cgccctgctg cccctcggca tgccggcggt
     4801 gctgctgggc ggcatctacg gcggggtgtt cacgccgacc gaagccgcgg cagtggccgc
     4861 gctctatgcg ctggtgctcg ccggcgtggt gtaccgagag ctctcggccg gcagcgtg
//
LOCUS       DASYJA010000084         3807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3100960, whole genome shotgun sequence.
ACCESSION   DASYJA010000084 DASYJA010000000
VERSION     DASYJA010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3100960"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VGO02_01785"
     CDS             <1..415
                     /locus_tag="VGO02_01785"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEV7476447.1"
                     /translation="ENEVPFKPKPVPKLVEKKAEAKKAEAAPTEARIEKKTVEKTVPA
                     KPAAPKPPPAERARAEAALAGVEYVVQVGVFADPAAAIDKLKAANLPYYTETLKDNLT
                     RVRAGPFLTKEAAEKTRERLKGLGLDPGTVTTRAG"
     gene            412..900
                     /locus_tag="VGO02_01790"
     CDS             412..900
                     /locus_tag="VGO02_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEV7476448.1"
                     /translation="MTWVDLAFLGVLAISIGWGVWRGLVHEMLSLGGWILAFLAANLF
                     AGPLSEAITAAMQPELRVLLAWLGIFVVVLLIASLFGMLLRRFIKTVGLASTDRVLGG
                     VFGLARGLLIGLVFALLAGLTRFPSHPAWRDSFFGPPLANTVIQLKPWLPAGLAQRLR
                     YH"
     gene            931..2445
                     /gene="purF"
                     /locus_tag="VGO02_01795"
     CDS             931..2445
                     /gene="purF"
                     /locus_tag="VGO02_01795"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEV7476449.1"
                     /translation="MCGILGIVAHSPVNQLLYDELLLLQHRGQDAAGIVTSEHNSFHM
                     HKGGGMVRDVFRTRNMRSLPGNLGIAHCRYPTAGSAFKVAEAQPFYVNSPFGIVLGHN
                     GNLINSEQLKEEMFRTDRRHVNTGSDSEVLVNVLAHELEQASVKLRLDPAAIFAAVAN
                     VHRRVRGAYAVVAMIAGYGVLAFRDPFGIRPLVIGYNETPAGTEYMVASESVAVDALG
                     FRVLRDVAPGEAVFIAEDGRFDARQCAPHASLNPCIFEYVYLARPDSLIDGASVYETR
                     LHMGEKLAEKIRRQYRHLQVDVVIPIPDSSRPSALQLAAGLGVPYREGFVKNRYIGRT
                     FIMPGQAVRSRSVRQKLNPMSMEFSGKNVLLVDDSIVRGTTSREIVQMARESGARKVF
                     FASAAPPVRFPNVYGIDMPTRAELIAAHRSEDEVTRELGADALIYQDLAALKDAVRQA
                     NPRLANFETSCFDGVYVTGDVTSDYLRAIEIHRDAARDSAEDAETAQLDLNLAA"
     gene            2561..3730
                     /locus_tag="VGO02_01800"
     CDS             2561..3730
                     /locus_tag="VGO02_01800"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEV7476450.1"
                     /translation="MDKYQIATQSVRAGQVRTQFNEHAEALFLTSSFVFDNAAQAAAR
                     FSGGEEGMVYSRFTNPTVQMFQERLAALEGAETCVATASGMSAILTAAMSHLKSGDHV
                     VCSKAVFGATIQLFSTILARFGIETTYVSPTRVEEWQKAVKRNTRLFFLETPSNPLTE
                     VSDIGALSAVAKRAGALLAVDNVFCTPALQRPLELGADMVIHSATKYLDGQGRVIGGA
                     IAGPKAVVADAMIPFLRTAGPSMSPFNAWVLLKGLETLELRMNAQSASALALARWLES
                     HKSVARVFYPGLVSHPQHELAKRQQRAAGAVLSFEVKGGRAAAWRVIDSAQLMSITAN
                     LGDVKTTLIHPATTTHGRISQEARDAAGISDGLLRISVGLEALSDLQADLTRGLA"
BASE COUNT          625 a         1263 c         1269 g          650 t
ORIGIN      
        1 cgagaacgag gtgcctttca agcccaagcc ggtgccgaaa ctggtcgaga agaaggccga
       61 ggcaaagaag gccgaagcgg ccccaaccga agcaagaatc gagaaaaaaa cagtagaaaa
      121 gaccgtcccg gccaagcccg cggcgcccaa gccgccaccc gccgagcgcg cgcgcgccga
      181 agccgcgctg gccggcgtcg aatatgtggt gcaggtgggt gttttcgccg atccggcggc
      241 agccatcgac aagctgaagg cggcaaacct gccgtactac accgagacgc tgaaggacaa
      301 cctgacacgg gtgcgcgccg gtccgtttct gaccaaggaa gcagctgaaa agacccgcga
      361 gcggctgaag ggtctcggcc tcgatccggg aaccgtgacg acccgcgccg gatgacctgg
      421 gtcgacttgg cctttctcgg cgtcctcgcg atctcgatcg gctggggcgt gtggcgcggc
      481 ctggtgcacg agatgctctc gctgggcggc tggattctcg ccttcctcgc ggcgaacctc
      541 tttgccgggc cgctgagcga ggcgatcacc gccgccatgc agccggagct gcgggtgctg
      601 cttgcctggc tgggcatctt cgtggtggtg ctgctgatcg ccagcctgtt cgggatgctg
      661 ctgcgcagat tcatcaagac tgtcggcctc gcctccaccg accgcgtgct cggcggggtg
      721 ttcggcctgg cgcgcggcct gctgatcggc ctggttttcg cgctgctcgc cgggctcacg
      781 cgctttcctt cccatcctgc ctggagggat tccttcttcg gcccgccgct cgccaacacg
      841 gtcatccagc tcaagccctg gctgcccgcc gggctcgccc agcgcttgcg atatcattga
      901 cctttaacgg caccggggag tcttagtttc atgtgcggga ttctcggcat cgtcgctcat
      961 tcgcccgtaa accagctgct ttacgacgag ctgctgctgc tccagcaccg cggccaggac
     1021 gctgcgggga tcgtcaccag cgagcacaac tccttccaca tgcacaaggg cggcggcatg
     1081 gtgcgcgacg tgttccgcac gcgcaacatg cgctcgctgc cgggcaatct tggcatcgcg
     1141 cactgccgct acccgaccgc gggctcggcg ttcaaggtgg cggaagcgca gccgttctac
     1201 gtcaactcgc ccttcggcat cgtcctgggc cacaacggca acctgatcaa ttccgagcag
     1261 ctcaaggaag agatgttccg caccgaccgg cggcacgtca acaccggctc ggattccgag
     1321 gtgctggtca acgtcctggc gcacgagctc gagcaggcct cggtcaagct gcgcctggat
     1381 ccggcggcca ttttcgcggc agtggcgaat gtgcaccggc gagtgcgtgg cgcctacgcg
     1441 gtggtggcga tgatcgcggg ctacggcgtg ctcgcgttcc gcgacccttt cggtatccgc
     1501 cccctggtga tcggctataa cgagaccccg gcgggcaccg agtacatggt ggcgtcggag
     1561 tccgtcgcgg tggatgcgct cggcttccgc gtgctgcgcg acgtcgcccc cggagaagcg
     1621 gtgttcatcg ccgaggacgg caggttcgac gctcggcagt gcgcgccgca cgccagcctc
     1681 aatccctgca tcttcgagta cgtctacctg gcgcgccccg attcgctgat cgacggcgcc
     1741 tcggtctacg agacgcggct tcacatgggc gagaagctgg ccgagaagat ccgccgccag
     1801 taccgccacc tgcaggtcga cgtggtgatc ccgattcccg attccagccg gccctccgcg
     1861 ttgcagctcg ccgccggcct gggcgtgccg taccgcgaag ggttcgtgaa gaaccgctac
     1921 atcggccgca ccttcatcat gccgggccag gcggtgcgct cgcgttcggt gcggcagaag
     1981 ctgaacccga tgtcgatgga gttcagcggc aagaacgtgc tgctggtgga cgactcgata
     2041 gtcaggggca cgaccagccg cgagatcgtg cagatggcgc gcgagtcggg ggcgcgcaag
     2101 gtgttcttcg cctcggcggc gccgccggtg cgctttccca acgtctacgg catcgacatg
     2161 ccgacgcgcg ccgagctgat cgccgcccac cgcagcgagg acgaggtgac gcgggagctc
     2221 ggggcggatg ccctgatcta ccaggacctc gcggccctca aggacgccgt gcggcaggcg
     2281 aacccccgcc tggcgaactt cgagacctcg tgcttcgacg gtgtttatgt cacgggcgac
     2341 gtcaccagcg actacctgcg cgccatcgag atccatcgcg atgccgccag ggattcggct
     2401 gaggatgcgg aaacagcaca gctcgatctg aatctggcag cctgatatac tttcgtctgg
     2461 tcgcgccgat ttgagccaca gcttgcgggc gcgtaataaa tgccgctaaa gcgtgccgag
     2521 agaacccggt ccgcttacac gagagaggcc gggttttttt atggataagt atcagatcgc
     2581 aacccagagc gtgcgcgccg gtcaggtgcg cacgcagttc aacgagcacg ccgaggcgct
     2641 gttcctcacc tcgagctttg tcttcgacaa cgccgcgcag gcggcggcgc gcttcagcgg
     2701 cggcgaagag gggatggtct attcccgctt caccaacccc accgtgcaga tgttccagga
     2761 gcgcctggcg gcgctggagg gcgccgagac ctgcgtcgcc accgcctctg gaatgtcggc
     2821 gatccttacc gcggcgatgt cgcacctgaa aagcggcgac catgtcgtct gctcgaaggc
     2881 ggtgttcggc gccaccattc agctcttcag caccatcctt gcgcgcttcg gcatcgagac
     2941 gacctatgtc tcgccgacgc gggtggagga gtggcagaag gcggtcaagc gcaatacgcg
     3001 gctttttttc ctcgagacgc cttccaaccc gctgaccgag gtttccgaca tcggcgcgct
     3061 gtccgcggtg gcaaagcgcg ccggagccct gctggcggtc gacaacgtgt tttgcacgcc
     3121 cgcgctgcag cggccgctcg agctgggcgc cgatatggtg atccactccg ccaccaaata
     3181 cctcgacggc cagggccggg tgatcggagg ggcgatcgcc ggcccgaagg cagtagtggc
     3241 cgacgcgatg atcccgtttc tgcgcaccgc cgggccgtcg atgtcgccgt tcaacgcctg
     3301 ggtgctgctg aaaggcctgg agacgctcga attgcgcatg aacgcacagt cggcatcggc
     3361 tctcgcgctg gcgcgctggc tcgagtcgca caagtcggtg gcgcgcgtgt tctaccccgg
     3421 gctggtgtcg catccgcagc acgagttggc gaagcgccag cagcgcgcgg cgggtgccgt
     3481 cctgtcgttc gaggtgaagg gagggcgtgc cgcagcgtgg cgggtgatcg attcggcgca
     3541 gctgatgtcg atcaccgcca acctcggcga cgtgaagacc accctgatcc atccggccac
     3601 caccacgcac gggcggatct cccaggaagc acgcgacgcc gcgggcatca gcgacggcct
     3661 gctgcgcatc tcggtcgggc tggaagcgct ctccgacctg caggcagacc tgacacgggg
     3721 cctggcgtag gataggcctc atgccgagcg ggccgcgcaa gtcggttcag gcggcccgga
     3781 tcggcctgct tgccgtagcg tattggg
//
LOCUS       DASYJA010000085         6569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1194156, whole genome shotgun sequence.
ACCESSION   DASYJA010000085 DASYJA010000000
VERSION     DASYJA010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194156"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..124)
                     /locus_tag="VGO02_01805"
     CDS             complement(<1..124)
                     /locus_tag="VGO02_01805"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV7476451.1"
                     /translation="MGVVNVTPDSFSDGGKFLDPRVALGHAFKLAEEGADILDIG"
     gene            complement(205..2091)
                     /gene="ftsH"
                     /locus_tag="VGO02_01810"
     CDS             complement(205..2091)
                     /gene="ftsH"
                     /locus_tag="VGO02_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV7476452.1"
                     /translation="MLKNLVIWLVIGLVLMTVFNQFSTRQDSRAVPMEYSEFMEAAKQ
                     GQISKVEIQGRTIRATTQEKKQIVVNSPGLYDIWMVGDLMKYGVKVSTKPEEEQSMLM
                     QIFISWFPMLLLIGVWIFFMRQMQGGGRGGAFSFGKSRARMLDESTNTVTFADVAGCE
                     EAKEEVSELVEFLRDPSKFQKLGGRIPRGVLMVGSPGTGKTLLARAIAGEAKVPFFSI
                     SGSDFVEMFVGVGAARVRDMFEQAKKHSPCIVFIDEIDAVGRQRGAGLGGGNDEREQT
                     LNQLLVEMDGFEATMGVIVIAATNRPDVLDPALMRPGRFDRQVVVPLPDIRGRTEILK
                     VHMRKVPLAPDVVPDIIARGTPGFSGADLANIVNEAALFAARGNKRLVDMEDFEHAKD
                     KIMMGAERRSMVMSEEERKTTAYHESGHAVVGRLLPRADPIHKVTIIPRGRALGVTFF
                     LPEKDRYGLDRDQMLAQITVSFGGRVAEELFLNQKTTGARQDFESATAIARDMVTRYG
                     MSDELGPMVYGENDGEIFLGRQVTTHKNVSEATLQKVDAEIRRIIDEQYMLARKLIDG
                     NREKVEVMAKALLEWETLDAEQVNDIMQGRPPRPPKPSAGTSSTPSTPPGGEQGVVAP
                     HPAA"
     gene            complement(2142..2747)
                     /locus_tag="VGO02_01815"
     CDS             complement(2142..2747)
                     /locus_tag="VGO02_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEV7476453.1"
                     /translation="MAKSNKAWLQRHVNDHYVKKAKAQGYRSRAAFKLLELDEKERLL
                     RPGMTVVDLGAAPGGWSQVAARKVAPGGKVIAIDLLEIAPISGVTVLKADFREKVLLD
                     SLAGRQADAVLSDVSPNLSGIGNVDQARILELTGAAIDFCRQGLKPEGWFLVKAFHGE
                     AFGEITARLKKTFARVKVIKPSASRGESAETYVLARGLTGL"
     gene            complement(2917..3141)
                     /locus_tag="VGO02_01820"
     CDS             complement(2917..3141)
                     /locus_tag="VGO02_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476454.1"
                     /translation="MKRTLIAALLAMSAATVFAANSQYSNEAGEQDLHTPIAKSDDYR
                     NDAGEQDQHQPQVADAGDKGAQDQNFPQVG"
     gene            complement(3236..3733)
                     /locus_tag="VGO02_01825"
     CDS             complement(3236..3733)
                     /locus_tag="VGO02_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476455.1"
                     /translation="MDQRTLQDSAAAAERYGEVTLGVECAWPQVSGAPTAVQGFSLSP
                     SSSPKRDSGVGVAFSLVAALLLGTALAAGAAPYAAEEYDFSELSSMGPAALGIVESVR
                     EVPLRTAPAELANVFEHSLNAETGEEVVVRLDDGSTVTVQLVGIQHFRPGERVRVLGG
                     RVLRT"
     gene            complement(3801..4199)
                     /locus_tag="VGO02_01830"
     CDS             complement(3801..4199)
                     /locus_tag="VGO02_01830"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEV7476456.1"
                     /translation="MLRLTYRRALPEREDWWMPTHLQLGASAWRVPDIGGSTQRFDLN
                     ATPIWRAERPWGYVEAGIGVYLLSKTINNDANHLPSSFQFGSHLGAGVHFGERGQGRA
                     GIALQHLSNAGLKQPNGGINLVLATLSFDF"
     gene            complement(4256..5641)
                     /locus_tag="VGO02_01835"
     CDS             complement(4256..5641)
                     /locus_tag="VGO02_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEV7476457.1"
                     /translation="MKKQLLVLFLLLAGCATSLPPIDPAQVPTAPEAFKEGDGRWTVA
                     PPAEAQPRGEWWRAFNDPVLDDLVERAAAANTSIQVAASRLGQARAFLRATDAERSPQ
                     VNVGASAARAQGIVGGNAGPARSLFLAGADFSYEVDLFGRLSHATNAARLDAQSREAL
                     LQSTRLLVQSDVAQTYLALRALDEERSLVRTTVAAYRETLSLTERRWRAGDVAELDVA
                     RANTEVAATESEALALDRRRSELEHALAVLVGEVPSRFSVAADEWRSALPVIPAGVPS
                     TVLTRRPDVSAAQQAMLAAQSRVGVAKAAYFPRVALTGFAGYGSTDLSDLFEWSSRAW
                     LLGAVAALPIIDGGRRKAGIEASGAELEGEVARYREQILVAFKDVEDQLAALRLLAEQ
                     AEAQGRAVTSASRSTALSDVRYRNGYVSQLDLLDAQRSELRNRRQALQVRSAQYQSTV
                     ALVRALGGSWN"
     gene            complement(5638..5802)
                     /locus_tag="VGO02_01840"
     CDS             complement(5638..5802)
                     /locus_tag="VGO02_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476458.1"
                     /translation="MAVAASALIATTASAWALDGRSRLVVALRAPPAEVHITPTVTNA
                     NPGDPKVLFP"
     gene            complement(5820..>6569)
                     /locus_tag="VGO02_01845"
     CDS             complement(5820..>6569)
                     /locus_tag="VGO02_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV7476459.1"
                     /translation="NGFLSADINGRNAPGFSSGQAQDAVERIAAETLPPGISYEWTEI
                     TYQEILAGNSAVWVYPLAILLVFLVLAALYESLALPLAIILIVPMGLLAALTGVWLTG
                     GDNNIFTQIGLVVLVGLSAKNAILIVEFARELEFAGRTPLQAAIEASRLRLRPILMTS
                     IAFVMGVLPLAYATGAGAEMRQAMGIAVFAGMIGVTAFGIFLTPVFYVLLRALSGNRP
                     LKRHGEVPHDAEAFAGGGEVRPLPAAPKEDA"
BASE COUNT         1027 a         2228 c         2211 g         1103 t
ORIGIN      
        1 cgccgatatc gagaatatcg gcaccctcct ccgccagctt gaacgcgtgc ccgagggcga
       61 cccgcgggtc gaggaacttg ccgccgtcgg aaaaggaatc gggcgtgaca ttcaccacgc
      121 ccattaccag aggcctgctc aggtcgagcc ggaaccggcc cgcggccaag aggcccgcta
      181 cgccgcgcaa tgcggcgttc ccactcacgc cgctggatgc ggtgccacta ctccttgctc
      241 tccgcccgga ggcgtcgacg gcgtgctcga ggtgccggcg ctgggcttgg gcggccgcgg
      301 cggacggccc tgcatgatgt cgttcacctg ctcggcgtcg agcgtctccc actcgagcag
      361 cgccttggcc atgacctcga ccttctcgcg gttcccgtcg atcagcttgc gcgccagcat
      421 gtactgctcg tcgatgatgc ggcggatctc ggcatcgacc ttctgcagcg tggcttccga
      481 cacgttcttg tgcgtggtca cctggcggcc gaggaagatc tcgccgtcgt tctcgccgta
      541 caccatcggc ccgagctcgt cggacatgcc gtagcgcgtc accatgtcgc gcgcgatcgc
      601 ggtcgcgctc tcgaagtcct ggcgcgcgcc ggtggtcttc tggttgagga acagctcctc
      661 cgcgacgcgg ccgccgaagg acaccgtgat ctgcgcgagc atctggtcgc ggtccaggcc
      721 gtagcggtcc ttctccggca ggaagaaggt gacgccgagc gcgcggccgc gcgggatgat
      781 ggtgaccttg tggatcgggt cggccctggg cagcagcctg ccgaccacgg cgtggccgga
      841 ctcgtggtac gcggtggtct tgcgctcttc ttccgacatc accatcgagc gccgctcggc
      901 gcccatcatg atcttgtcct tggcgtgctc gaagtcttcc atgtcgacca ggcgcttgtt
      961 gccgcgggcg gcgaacaggg ccgcctcgtt gacgatgttg gcgaggtccg cgcccgagaa
     1021 acccggcgtg ccgcgcgcga tgatgtcggg caccacgtcg ggcgcgagcg gcaccttgcg
     1081 catgtgcacc ttgagaatct ccgtgcggcc gcggatgtcg ggcagcggca ccaccacctg
     1141 gcggtcgaag cgacccgggc gcatcagcgc cggatcgagc acgtcgggcc ggttggtcgc
     1201 ggcgatcacg atcacgccca tcgtcgcctc gaagccgtcc atctcgacca gcagctggtt
     1261 caatgtctgc tcgcgctcgt cgttgccgcc gcccaggccg gcgccgcgct ggcggccgac
     1321 ggcgtcgatc tcgtcgatga acacgatgca gggcgaatgc ttcttcgcct gctcgaacat
     1381 gtcgcgcacc cgcgccgcgc ccacgccgac gaacatctcg acgaagtcgg agcccgagat
     1441 ggagaagaac ggcactttgg cttcgcccgc gatggccctg gcaagcaggg tcttgcccgt
     1501 gccgggtgag ccgaccatca gcacgccgcg cgggatgcgg ccgccgagct tctggaactt
     1561 ggacggatcg cgcaggaact cgaccagctc ggagacttcc tccttcgcct cctcgcagcc
     1621 ggcgacgtcg gcgaaggtga cggtgttggt cgactcgtcg agcatgcgcg cgcgcgactt
     1681 gccgaagctg aacgcgccgc cgcgcccgcc gccctgcatc tggcgcatga agaagatcca
     1741 cacgcctatg aggagcagca tcgggaacca cgagatgaag atctgcatca gcatcgactg
     1801 ctcttcctcg ggcttggtcg acaccttgac gccgtacttc atcaggtcgc cgaccatcca
     1861 gatgtcgtac aggccgggcg aattgacgac gatctgcttc ttttcctgcg tggtggcgcg
     1921 gatggtgcgc ccctggatct cgaccttgct gatctgtccc tgcttcgccg cctccatgaa
     1981 ctcggagtac tccatgggaa cggcgcgcga gtcctggcgc gtgctgaact ggttgaacac
     2041 ggtcatcagc accagcccga tgacgagcca gatgaccagg ttcttaagca tattgttcaa
     2101 tgcgttactc ctctttctgc cgcgctgcgt cgagacccat cttacaggcc ggtcagaccc
     2161 cgcgccagca cgtaggtctc cgccgattca ccccgtgatg cggagggttt tatgactttg
     2221 acccgggcga atgttttctt caatcgcgcc gtaatttcgc cgaaagcctc gccgtggaac
     2281 gccttcacga ggaaccaccc ttccggcttg agcccctggc ggcagaaatc aatcgccgca
     2341 ccagtcagtt cgagtatgcg ggcctggtcg acgttcccga ttccagacaa attcggcgaa
     2401 acgtcggaaa gaaccgcgtc ggcctggcgg ccggcgagac tgtcgaggag cactttttca
     2461 cggaaatcgg ccttgagcac cgtcaccccg gagattgggg cgatttcgag cagatcaatc
     2521 gcgatcacct tgccgccggg cgcgaccttc ctggccgcca cctgggacca gccgccgggc
     2581 gcggcgccca ggtcgacgac cgtcatgccg gggcgcagca gcctttcctt ctcatccagc
     2641 tcgaggagct tgaacgccgc gcgcgagcgg tagccctgcg ccttggcttt cttgacgtag
     2701 tgatcgttga cgtggcgttg cagccaggcc ttgttcgact tggccatgcg tcaatggtac
     2761 tgcggggaga actgccgccg ccctgtgcct caagagccag agcagaggga ccaggatgaa
     2821 tccgccgggc agcagaagag ccgccagcag gtgaggactg aagcggcgcg cggagtggag
     2881 gaagtctttc acgcgcgccg cttcctgggt attgcgttag ccgacttggg gaaagttctg
     2941 gtcctgcgcg cccttgtcgc ctgcgtcggc gacctggggc tggtgctggt cctgctcgcc
     3001 cgcgtcgttg cggtagtcgt cgcttttggc gatcggcgtg tgcaggtcct gctcgccggc
     3061 ttcgttgctg tactggctgt tggcggcgaa gaccgtggcc gcggacatcg cgaggagcgc
     3121 tgcaatcaga gtgcgtttca tgttcattcc tttcagggtt tggagattgt ctttgctgct
     3181 ggaaccactt tgcaggaccc gcgtatcgca ggcattgcga aatcctcggg cggcgttacg
     3241 tccgcaacac gcgcccgccc agcacgcgca cgcgctcgcc gggccggaag tgctgtatgc
     3301 cgaccagttg cactgtcacc gtgctgccgt cgtcgagccg cactaccacc tcctcgccgg
     3361 tttcggcgtt gaggctgtgc tcgaatacgt tggcgagctc cgcgggagca gtgcgcagcg
     3421 gcacctcgcg cacgctctcc acgatgccca aagcagccgg gcccatgctc gacagctcgc
     3481 tgaagtcgta ctcctccgcc gcgtagggcg ccgcgccggc ggccagcgcc gttccaagga
     3541 gcagcgctgc gactagagaa aaggcgaccc cgaccccaga gtctcttttg ggggaggagg
     3601 agggagagag tgagaaaccc tgaactgcgg tcggggcgcc ggaaacctgg ggccacgcgc
     3661 actccacgcc gagtgtcacc tcgccgtagc gctccgcggc tgccgcggag tcctgaagag
     3721 tgcgctgatc catgcctgca tgttgggcca tcgctgtatc cggcatgtgt cgcgctgttg
     3781 cgcctgcgtt acagatgcaa ctagaaatcg aaactgagcg tcgccagcac caggttgatg
     3841 ccgccgttcg gctgcttgag gccggcgttc gacaggtgct ggagcgcgat cccggcgcgg
     3901 ccctgcccgc gctcgccgaa gtgcacgccg gcccccaggt gcgagccgaa ctggaacgaa
     3961 gacggcaggt ggttcgcgtc gttgttgatg gtcttcgaca gcaggtagac gccgatcccg
     4021 gcctcgacgt atccccacgg acgctcggcg cgccagatcg gggtcgcatt caggtcgaag
     4081 cgctgcgtgc tgccgccgat atcgggcacg cgccaggcgc tcgcgcccag ctgcaggtgc
     4141 gtgggcatcc accagtcttc gcgctccggc agcgcgcgcc ggtacgtcag cctgaggatg
     4201 tccgtctccg tttcatcccc gcccctcccg cgctacacct gacaaacggc tgtaactagt
     4261 tccagctgcc gccgagcgcc ctcaccagtg cgacggtgct ctggtactgc gccgagcgga
     4321 cctgcagggc ctggcggcgg ttgcgcagct cgctgcgctg cgcgtcgagc aggtcgagct
     4381 ggctgacgta gccgttgcgg tagcgaacgt cggacagcgc cgtcgagcgg ctcgcagagg
     4441 tgaccgccct gccctgtgcc tccgcctgct cggcgagcag gcggagcgcc gcgagctggt
     4501 cctcgacgtc cttgaaggcg accagtatct gctcgcggta acgggccacc tcgccttcca
     4561 gctcggcgcc cgaggcttcg atgccggcct tgcggcggcc gccatcgatg atcggcaggg
     4621 ccgccacggc acccagcagc caggcgcgcg aagaccattc gaaaaggtcg ctcaggtcgg
     4681 tggacccata gccggcaaag ccggtcagag ccaccctcgg aaagtaggcc gccttcgcca
     4741 cgccgacgcg cgactgtgcc gcgagcatcg cctgctgcgc cgccgagacg tcgggacgac
     4801 gcgtaagcac tgtgctcggc acgccggccg gaatgaccgg cagcgcgctg cgccattcgt
     4861 ccgccgcgac tgagaagcgc gacggaacct cgcctacgag cacggcgagg gcatgctcga
     4921 gctcggagcg acggcggtcg agcgccagcg cctccgactc ggtcgccgcc acctcggtgt
     4981 tcgcgcgcgc gacgtccagc tcggccacgt ctcccgcccg ccagcgccgc tcggtgagcg
     5041 acagcgtttc gcgataggcg gctacagtag tcctgaccag agacctctct tcatccaggg
     5101 cacgcagcgc aagataggtc tgcgctacgt ccgactggac aagaaggcgg gtgctctgga
     5161 gcagcgcctc gcgcgactgg gcgtcgagac gcgcggcatt cgtcgcgtgc gacagccgtc
     5221 cgaagagatc cacttcgtag gagaagtcgg cgcccgcgag aaacaggctg cgcgccggac
     5281 ccgcgttgcc gccaactatg ccctgcgcac gcgcggcgct ggcgccgacg ttgacttgcg
     5341 gcgagcgctc ggcgtcggtg gcgcgcagga acgcgcgcgc ctgccccagc cgcgatgcgg
     5401 cgacctggat gctcgtattg gcggccgccg cgcgctcgac caggtcgtcg agcaccgggt
     5461 cgttgaacgc ccgccaccac tcgccgcgcg gctgcgcctc ggccggcggc gccacggtcc
     5521 agcggccgtc accttccttg aaggcctcgg gcgcggtagg tacctgcgcg gggtcaatcg
     5581 gcggtagcga ggtggcgcag ccggcgagca gcaggaacag aaccagtagt tgctttttca
     5641 tgggaagagg accttcgggt cgccggggtt cgcgttggtg acggtggggg tgatgtggac
     5701 ctctgccggc ggggcgcgca gcgcaaccac cagcctggag cggccatcga gggcccacgc
     5761 cgacgccgtt gtcgcgatga gcgcgctagc ggccacggcc attgcgcgat cgagtctcat
     5821 cacgcgtcct ccttcggcgc tgcaggcagc ggcctgactt cgccgccgcc ggcgaaggcc
     5881 tcggcgtcgt gcggaacttc gccgtggcgc ttgaggggac ggttgccgct cagcgcgcgc
     5941 agcagcacgt agaacaccgg cgtgagaaag atgccgaagg cagtcacgcc gatcatgccg
     6001 gcgaacaccg cgatgcccat cgcctggcgc atttccgcgc cggcgccggt ggcgtaggcg
     6061 agcggcagca cgcccatgac gaaggcgatc gaggtcatca ggatcgggcg caggcgcagc
     6121 cggctcgcct cgatcgccgc ctgcagcggg gtgcggccgg cgaactccag ctcgcgcgcg
     6181 aactccacga tcaggatcgc gttcttcgcc gacaggccga ccagcacgac cagcccgatc
     6241 tgggtgaaga tgttgttgtc gccgccggtg agccacacgc cggtgagcgc ggcgagcagg
     6301 cccatcggca cgatgaggat gatggcgagc ggcagcgcca ggctctcgta gagcgcggcg
     6361 agcaccagga acacgagcag gatggcgagc gggtacaccc acaccgcgga gttgccggcg
     6421 aggatctcct ggtaggtgat ctcggtccat tcgtagctga tgcccggcgg cagcgtctcg
     6481 gcggcgatgc gctccaccgc gtcctgcgcc tggcccgacg agaagcccgg agcgttgcgc
     6541 ccgttgatat cggccgagag gaagccgtt
//
LOCUS       DASYJA010000086         2180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2373664, whole genome shotgun sequence.
ACCESSION   DASYJA010000086 DASYJA010000000
VERSION     DASYJA010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2373664"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VGO02_01850"
     CDS             complement(<1..872)
                     /locus_tag="VGO02_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEV7476460.1"
                     /translation="MSAPQLPRATPEQLSTLTPLAGMSKERLAELAEVAVVERAARGA
                     DPLKGRLEAAQSIFLLSGELLLAFEGGGTLVVVGGASEETFQALNRQKAKLSRTKAVT
                     DVDLLAIDDEVLDILATWDQVAASGAGDGGSTMARAVRSDARLITGAFSLANLRAGAF
                     AQLPVAHIDELLKRFERVRTARGDMVIKEGDEGDFYYVIESGRFQVERLVGGAKVVLA
                     ELKGGDSFGEEALVSEAKRNASVIGLGEGELLRLRREDFNELLREPLLKRSGFEDAAE
                     RVRRGALWLDVRYPS"
     gene            complement(886..1653)
                     /locus_tag="VGO02_01855"
     CDS             complement(886..1653)
                     /locus_tag="VGO02_01855"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HEV7476461.1"
                     /translation="MRLRVLGCSGGIGGRHLRTTSFLVDGDILIDCGTGVGDLTLAEL
                     SQIDHIFITHSHLDHVASIPFLVDTVGGMRSKPLTVYATRATIEILKNQLFNWAIWPD
                     FTEIPNAEAPFLRYQEIEVGNTLSLSGREITPLPARHTVPAVGYHLDSGQASLVFTGD
                     TGQNDALWRVVNRIRNLKILIIETAFSNKERQLAEISRHLCPETLAEELAKLERTAEI
                     YITHLKPGEIEPTMLEIEECAGTFRPRMLQNNLVLEF"
     gene            complement(1661..>2180)
                     /locus_tag="VGO02_01860"
     CDS             complement(1661..>2180)
                     /locus_tag="VGO02_01860"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7476462.1"
                     /translation="EKFRARGWPEFNIGVGLNSGPVRVGDMGSKVRKAYTAMGDAVNL
                     ASRLEGRTKTYGVGILVGEATRELVKDVVYKELDLIRVKGKDTAVRVFEPIGLESEVD
                     KKTQDELKLWHQTLRAYRAQQWDQIEVNLINLQRMNPPCEVYKLFAERVNGFRRTPPP
                     ANWDGAWTFEEK"
BASE COUNT          314 a          749 c          740 g          377 t
ORIGIN      
        1 gaggggtagc gcacgtcgag ccagagggcg ccgcgccgca cccgctcggc ggcgtcctcg
       61 aagccgctgc gcttgagcag cggctcgcgc agcagctcgt tgaagtcctc ccgccgcagg
      121 cgcagcagct cgccctcgcc caggccgatc acgctcgcgt tcctcttggc ctcggacacc
      181 agcgcctcct cgccgaagga gtcgccgccc ttcagctcgg cgagcaccac cttggcgccg
      241 ccgaccaggc gctcgacctg gaagcgcccc gattcgatga cgtagtagaa gtcgccctcg
      301 tcgccttcct tgatcaccat gtcgccgcgt gcagttctta cacgctcgaa gcgcttgagc
      361 agctcgtcga tgtgcgccac cggcagctgg gcgaaggcgc cggcgcgcag gttggcgagc
      421 gagaaggcgc cggtgatgag ccgcgcgtcg ctgcgcaccg cgcgcgccat ggtcgagccg
      481 ccgtcgccgg cgccgctcgc cgccacctgg tcccaggtgg cgaggatgtc gagcacctca
      541 tcgtcgatgg cgagcaggtc gacgtcggta accgccttgg tgcgcgacaa cttcgctttt
      601 tgcctgttca acgcctggaa ggtctcctcg ctggcaccgc ccaccaccac cagcgtgccg
      661 ccgccctcga aggcgagcag cagctccccc gagagcagga agatcgactg cgccgcttcc
      721 agccggccct tcagcggatc ggcgccgcgc gccgcgcgct cgaccaccgc cacctcggcc
      781 agctcggcga ggcgctcctt cgacatcccg gcgagcgggg tcagcgtcga gagctgctcc
      841 ggagtggcgc gcggcagctg cggggcgctc atgtcatttc ccggcctaga actccagcac
      901 caggttgttc tgcagcatgc gcggcctgaa ggtgcccgcg cactcctcga tctcgagcat
      961 ggtgggctcg atctcgcccg gcttgaggtg ggtgatgtag atctcggcgg tgcgctcgag
     1021 cttggcgagc tcctcggcca gggtctcggg gcacaggtgg cgcgagatct cggcgagctg
     1081 gcgctccttg ttggagaacg cggtctcgat gatcaggatc ttcaggttgc ggatgcggtt
     1141 gaccacgcgc cagagcgcgt cgttctggcc ggtatcgccg gtgaacacca ggctcgcctg
     1201 gccgctgtcg aggtggtagc cgaccgccgg cacggtgtgg cgcgcgggca gcggcgtgat
     1261 ctcgcggccc gagagcgaca gcgtgttgcc gacctcgatc tcctggtagc gcaggaacgg
     1321 cgcctcggcg ttcgggatct cggtgaagtc cggccagatc gcccagttga acagctggtt
     1381 cttcaggatc tcgatagtgg cgcgcgtggc gtagacggtg agcggcttgc tgcgcatgcc
     1441 gccgacggta tcgacgagga acgggatcga ggcgacgtgg tcgaggtgcg agtgggtgat
     1501 gaagatgtgg tcgatctgcg acagctcggc cagcgtcagg tcgccgacgc cggtgccgca
     1561 gtcgatcagg atgtcgccgt cgaccaggaa cgacgtggtg cgcaggtggc gcccgccgat
     1621 tcctcctgag caaccgagca cacgcaggcg cattaccggc ctatttctct tcgaacgtcc
     1681 acgcgccgtc ccagttggca ggcggcggcg tgcggcggaa cccgttcacg cgctcggcga
     1741 acagcttgta cacctcgcac ggggggttca tgcgctgcag attgatgagg ttcacttcga
     1801 tctggtccca ctgctgggcg cggtaggcgc gcagcgtctg atgccacagc ttcagctcgt
     1861 cctgcgtctt cttgtccact tcgctttcaa gcccgatcgg ctcgaagacg cgcaccgcgg
     1921 tgtccttgcc cttgaccctg atcaggtcga gctccttgta gacgacatcc ttgaccagtt
     1981 cccgggtcgc ctcgcccacc aggatgccga cgccgtaggt cttggtgcgg ccttccagcc
     2041 gcgaggcgag gttcaccgcg tcgcccatcg cggtgtaggc cttgcgcacc ttcgagccca
     2101 tgtcgccgac gcgcaccggg ccggagttga ggccgacgcc gatgttgaac tccggccagc
     2161 cgcgcgcgcg gaacttctcg
//
LOCUS       DASYJA010000087         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2101898, whole genome shotgun sequence.
ACCESSION   DASYJA010000087 DASYJA010000000
VERSION     DASYJA010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2101898"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VGO02_01865"
     CDS             <1..393
                     /locus_tag="VGO02_01865"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476463.1"
                     /translation="EVADLIISKAAWKSLAPDVQEIFKMAATETYLRWWLSYSRENSK
                     VLAEMRTKHGVNVRRTPNEVLIKQLQTWDAIRDKFAKEDPFFAKVVESQRQYAANVVP
                     GRMAMYPAYSFSADYYWKQKPGAKPNGK"
     gene            394..>1516
                     /locus_tag="VGO02_01870"
     CDS             394..>1516
                     /locus_tag="VGO02_01870"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7476464.1"
                     /translation="MQRALDSVVDVLRVPASGDPSLAVLPVEARAGAQPEACEILIAG
                     GGTGGVAAALAAARSGRRVVLLEETDWLGGQMTAQGVSALDEHEHIESFGGTRSYYEL
                     RTRLREHYGVDNPGACWVTRLAFEPSVAVGVIQEMLRPHVDSGRLRIHLRKKVVAATT
                     HGDRITEVVAMGLDDSRLLRFHPELVIDATELGDLLPLCGAEHVIGAETAAQTGEPQA
                     QPVEAKPHCVQSFTYTFACERRAQGESHVISRPEKYEHYKAAQPYSLRIEVHGGEIYG
                     ETSGWLDYRLYDTMPGTKGGLWTYRRLIEGTRFSGSDLTMFNWPGNDYRDHSILECPP
                     LDAARALQDAKRVSLGFLYWLQTEAPATAARLGAPEIRLR"
BASE COUNT          279 a          488 c          509 g          240 t
ORIGIN      
        1 gaggtcgcgg atctcatcat ctcgaaggcg gcctggaagt cgcttgcgcc ggacgtccag
       61 gaaatcttca agatggccgc tacggaaacc tatctgcgct ggtggctcag ctacagccgg
      121 gagaattcca aagtgctcgc cgagatgcgc accaagcacg gcgtcaacgt cagacgcacg
      181 ccgaacgagg tgctgatcaa gcaattgcag acctgggatg cgatccgcga caagtttgcc
      241 aaagaggacc ccttcttcgc gaaggtggtg gagtcccagc ggcagtacgc cgccaacgtc
      301 gtgccgggcc gcatggccat gtacccggcc tatagctttt ccgctgatta ctactggaag
      361 cagaagccgg gggccaagcc gaacggcaag tgagtgcagc gcgcactcga ctcggtcgtc
      421 gacgtgttgc gagtgccggc gagcggcgat ccgtcgctcg ccgtgctgcc ggtcgaagcg
      481 cgggctggcg cgcaacctga ggcgtgcgag atcctgatcg ccggcggcgg caccggtggc
      541 gtcgccgctg cgcttgccgc ggccaggagc ggtcgccgcg tagtgctgct cgaggaaacc
      601 gattggctcg gcggccagat gactgcgcag ggcgtttccg cgctcgacga gcacgagcac
      661 atcgagtcgt tcggtggaac gcgcagctat tacgagctcc gcacgcgctt gcgcgagcac
      721 tacggcgtag acaatcccgg cgcctgctgg gttacgcggc tggcgttcga gccaagcgtt
      781 gccgtcggcg tgatccagga gatgctgcgg ccgcacgtcg actcgggtcg cttgagaatt
      841 cacctgagaa aaaaagtcgt ggccgcgacg acccatggcg atcgcatcac cgaggtggtg
      901 gcgatgggcc tcgacgacag ccgcctgctg cgcttccacc ccgaactggt catcgatgca
      961 accgagctcg gcgacctgct gccgttgtgc ggcgcggagc acgtgatcgg cgcggagacc
     1021 gcggcgcaga ccggcgagcc gcaggcgcag cccgtggaag caaagccgca ctgcgtgcag
     1081 agcttcactt acacgtttgc ctgcgaacgc cgtgcccagg gcgagagcca cgtcatttcc
     1141 cgcccagaga agtacgagca ctacaaggcc gcacagccgt acagcctgcg catcgaggtc
     1201 cacggcggcg agatctacgg tgagacgagc ggctggctgg actaccggct ttacgacacg
     1261 atgccgggga ccaagggcgg cctttggacc taccgccgcc tcatcgaggg aacgcgcttt
     1321 tcaggcagcg acctgacgat gttcaactgg ccgggcaacg actatcgtga ccacagcatc
     1381 ctcgagtgcc cgcctctcga tgccgcgcgc gccttgcagg acgcgaagcg ggtaagcctc
     1441 ggcttcctgt attggctgca gactgaagcc ccggcgacgg cagcgcgcct cggcgcgccc
     1501 gagatacgtc tgcgtc
//
LOCUS       DASYJA010000088         2403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1013491, whole genome shotgun sequence.
ACCESSION   DASYJA010000088 DASYJA010000000
VERSION     DASYJA010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013491"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VGO02_01875"
     CDS             <1..278
                     /locus_tag="VGO02_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEV7476465.1"
                     /translation="IVDAAKPVFTRKKPESGTDVQQQIAELQAAVSQNAEYVKELAEQ
                     LQSTVSALEKSAEVADLRLRRATVFSAISVSISIAALLLAAFTLLRL"
     gene            275..1006
                     /locus_tag="VGO02_01880"
     CDS             275..1006
                     /locus_tag="VGO02_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7476466.1"
                     /translation="MTSAVSPDTQRDLAPTGKLRVGLNYGNFLLVLRDKPDGTPQGIA
                     PDLARELAKRLEMGVEFIKFEAAGKLADGVKTGAWDVAFLGNEPQRAAEIAFSPAYLE
                     IPVTYLVPAGSPIQALADVDKKGVRIAVADKSAYHLFMMRELKSAELVSADGIDGSYK
                     LFIDSKLDALAGLKPRLLTDQQKNPGSRVLDGQLTTVQQSIGVPKSHEAAARYLKEFA
                     EEMKKSGFVAEAIARHKVPGVAVAP"
     gene            1003..1368
                     /locus_tag="VGO02_01885"
     CDS             1003..1368
                     /locus_tag="VGO02_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEV7476467.1"
                     /translation="MILRLLAVWLINAVALMTVAYLMPSIQVSSFGAALAAALVLGLI
                     NAVIRPVLILLTLPVTILTLGLFIFVLNGLLFWFVGSFIQGFFVAGFWAGVFGAIVFS
                     LISWLLSALLLRSGEPRAR"
     gene            complement(1304..>2403)
                     /locus_tag="VGO02_01890"
     CDS             complement(1304..>2403)
                     /locus_tag="VGO02_01890"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEV7476468.1"
                     /translation="DEFVLLWNGLKSSEDAAVLAQRVHSILARPFTVEGRTLNVGVTI
                     GVAVYPGDGRDFAELLRNADAAMYDAKESGRGTFRFFSPALHERSATRLRLENELRSA
                     LARSELVLNWHPVIRGRARQGQVLHGRGQVVGAEALVRWRHPERGLLMPDEFVPLAEE
                     CGLIRSVGEWTLDRALSQIGAWQDLLPGKPWFALNVSAAELAQGDAYFERLRGALREN
                     RVDGSRIELEITERVIMSHLAGNFETLRRIGGLGVRFAVDDFGTGYSSLAYLRLLPVD
                     KLKIDKSFLRAIDSQPADEAIVRAIAALAKTLGIGIAGEGIESEAQLERLLALGCEEW
                     QGHFFSAPLEASELEALLISGAGRTGARKSS"
BASE COUNT          367 a          857 c          803 g          376 t
ORIGIN      
        1 ccatcgtcga cgcggcgaag ccggtattca cgcgcaagaa gcccgagtcc ggcaccgacg
       61 tgcagcagca gatcgccgag ctgcaggcgg cggtgtcgca gaacgcggag tacgtgaagg
      121 agctcgccga gcagctgcag agcacggtga gcgcgctgga gaagtcagcc gaggtggccg
      181 acctgcgcct gcgccgcgcc acggtgttct ccgcgatctc ggtttcgatc tcgatcgccg
      241 cgctcctgct ggccgcgttt acactgctga gactatgacc agcgccgttt ctcccgacac
      301 gcaaagagac ctcgccccga cgggcaagct gcgcgtcggc ctcaattacg gcaacttcct
      361 cctggtgctg cgcgacaagc ccgacggcac gccgcagggc atcgctccgg acctggcgcg
      421 cgagctggcg aagcgcctcg agatgggggt ggaattcatc aagttcgagg ccgccggcaa
      481 gctcgccgac ggcgtgaaga ccggagcgtg ggacgtggcc ttcctcggca acgagccgca
      541 gcgcgccgcc gagatcgcct tcagccccgc ctacctcgag atcccggtga cctacctggt
      601 gccggcgggc tcgccgatcc aggcgctcgc cgacgtcgac aagaaggggg tgcgcatcgc
      661 cgtggcggac aagagcgcct accacctgtt catgatgcgc gagctgaaga gcgcggagct
      721 ggtgagcgcc gacggcatcg acggctccta caagctgttc atcgactcca agctcgacgc
      781 gctcgccggc ctgaagccgc gcctgctcac cgaccagcag aagaacccgg gctcgcgcgt
      841 gctcgacggc cagctcacca ccgtgcagca gtcgatcggc gtgccgaaga gccacgaagc
      901 cgcggcgcgc tacctgaagg aattcgccga ggagatgaag aagtcgggat tcgtcgccga
      961 agccatcgcc cgccacaagg tgcccggggt ggcggtcgcg ccatgattct ccgcctgctc
     1021 gccgtatggc tgatcaacgc ggtcgcgctg atgacggtcg cctacctgat gccctcgatc
     1081 caggtctcga gcttcggcgc cgcgctggcc gcggcgctgg tgctgggcct gatcaacgcg
     1141 gtgatccgcc cggtgctgat cctgctcacg ctgccggtga ccatcctcac gctgggcctg
     1201 ttcatcttcg tgctgaacgg gctgctcttc tggttcgtcg gctcgttcat ccagggcttc
     1261 ttcgtcgccg gcttctgggc cggcgtgttc ggcgccatcg tcttcagctt gatttcctgg
     1321 ctcctgtccg ccctgctcct gagatcagga gagcctcgag ctcgctagct tccagcgggg
     1381 cgctgaagaa gtgcccctgc cactcctcgc agcccagggc gagcagccgc tcgagctgcg
     1441 cctcgctctc gatgccctcg ccggcgatgc cgatgcccag cgtcttggcg agcgcggcga
     1501 tggcgcgcac gatcgcctcg tcggccggct gcgagtcgat ggcgcgcagg aacgacttgt
     1561 ctatcttcag cttgtcgacc ggcagcaggc gcagataggc gaggctcgag tagccggtgc
     1621 cgaagtcgtc caccgcgaag cgcacgccca ggccgccgat gcgccgcagg gtctcgaagt
     1681 tgccggcgag gtgcgacatg atcacgcgct cggtgatctc cagctcgatg cgcgagccgt
     1741 ccacccggtt ctcccgcaag gcgccgcgca gcctttcgaa atacgcgtcg ccctgcgcca
     1801 gctcggccgc cgagacgttg agcgcgaacc acggcttgcc cggcagcagg tcctgccagg
     1861 cgccgatctg cgacagcgcc cggtccaggg tccactcgcc cacgctgcgg atcaggccgc
     1921 actcctcggc cagcgggacg aactcgtcgg gcatcagcag gccgcgctcg ggatggcgcc
     1981 agcgcaccag cgcctcggcg cccaccacct ggccgcggcc gtgcagcacc tgcccctgcc
     2041 tcgcccgccc ccttatcacc ggatgccagt tgaggaccag ctcgctcctc gccagcgcgc
     2101 tgcgcagctc gttttccagg cgcaggcgcg tcgcggagcg ctcgtgcagc gcgggcgaga
     2161 agaagcggaa ggtgccgcgt ccgctctcct tcgcgtcgta catggcggcg tcggcgttgc
     2221 gcaggagctc ggcgaagtcg cggccgtcgc ccgggtagac ggcgacgccg atggtgacgc
     2281 cgacgttgag ggtgcggcct tcgacggtga acggccgcgc caggatcgaa tgcacgcgct
     2341 gcgcgagcac cgcggcgtcc tccgacgact tgaggccgtt ccacagcagc acgaactcgt
     2401 cgc
//
LOCUS       DASYJA010000089         3045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1738591, whole genome shotgun sequence.
ACCESSION   DASYJA010000089 DASYJA010000000
VERSION     DASYJA010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738591"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VGO02_01895"
     CDS             complement(<1..399)
                     /locus_tag="VGO02_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476469.1"
                     /translation="MLQAGVLAAAAGLIVLVFQLGGADRFLADPFYDPARGGFWLKDT
                     WLLAVPAHTGLKWTMLGVWVLCLALGGRLRRGGLYMGAIAVVVLLIRELSPFSCPWDL
                     PEYGGGKPELGRCLPAAHPLTGFALFGLYLA"
     gene            complement(414..2351)
                     /locus_tag="VGO02_01900"
     CDS             complement(414..2351)
                     /locus_tag="VGO02_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEV7476470.1"
                     /translation="MKRIPPRFVPLFLAVAILLLVSAVTRLALSLRPEVAVGGFADWI
                     RVFGVGLTFDLIAACYMLAPLALWLALVPDRVARSWPHRALMVLLLAAVAFGAFLLAV
                     SEWLFWDEFGSRFNFIAVDYLLYTHEVLGNIWQSYPVGRVLLALAALAVVLAIALRRR
                     IWSYAGAPLSWRIALVAVGAWAAVFTGTLRWVDSDTKNFSARDNPNDLAGNGVYEFFA
                     ANYRNELDYDRHYATMPIEEALPLVRTALGAQEKGVERLVAPRGQERRMNVVLVSIES
                     LGAEFLGSYGNAKGITPNLDRLAKESLWFSRVYATGNRTVRGMEALSLALPPTPGQSI
                     VRRPGNDKLFSLGSVLEDKDYSVIFAYGGYGYFDNMNAFFEANDYRSIDRRAIPSGRV
                     KHETIWGVADEHLFDYVLDELDREKKVDARKPLFVHVMTTSNHRPYTYPADRIDIPSG
                     TSREGAVKYTDYAVGKFLQDARSRPWFDDTLFVITADHGANARGGSRIPVDKYLIPLF
                     VYAPKHVQPRRVDRLMSQIDIAPTVLGLLDLRYYTKFFGRDILHSPPASDRAFIANYQ
                     TLGYVRAGRMVLLQPKRKVEVFRVDDALTILGPVDDPQMLREAIAFYSVASYVFRSGL
                     YRDEEQIPPDKRAALLSRSGR"
     gene            2391..>3045
                     /locus_tag="VGO02_01905"
     CDS             2391..>3045
                     /locus_tag="VGO02_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476471.1"
                     /translation="MRRARFAICWLVVGYLVATPLRAADYDPNANPFGPLPDDSLPLP
                     RAEDMPPLLTRASEDSASAPSFAELEAAGAIIGRVLIQPHDIFDLDNPKENNALFRLA
                     NRLHVTTRPSVIERDVLFKPGDRVSVRIIDETERLLRTNRYLHEVLIRPVNYRDGVVD
                     IEVITRDTWSLDPGLSASRAGGANSNRLSLREHNLLGTGISAGISYSSNVDRAGTEVG
                     "
BASE COUNT          541 a         1028 c         1013 g          463 t
ORIGIN      
        1 ggcgagataa aggccgaaca aggcgaatcc cgtaagcggg tgcgcagccg gcaggcagcg
       61 gcccagctcg ggcttgccgc cgccgtactc tggcaggtcc catgggcacg aaaacgggct
      121 gagctccctg atcaacagga ccaccactgc aatagcgccc atgtagagcc caccgcggcg
      181 caggcggccg ccgagcgcga ggcacagcac ccagacaccg agcatggtcc acttgaggcc
      241 ggtatgggcc gggaccgcga gcagccaggt gtctttcagc cagaacccgc cgcgcgccgg
      301 gtcgtagaag ggatcggcga ggaaccggtc ggcgccgccg agctggaaaa ccagcacgat
      361 caggccggcc gccgccgcca gaacgccggc ttgcagcact gcccttcgca aagctagcgg
      421 ccgctgcgcg acagcagcgc tgcgcgcttg tccggcggga tctgctcctc gtcccggtac
      481 aggccgctgc ggaacacgta cgacgcgacc gagtagaagg cgatggcctc gcgcagcatc
      541 tgcggatcgt ccaccggccc gaggatggtc agggcgtcgt cgacgcgaaa cacctcgact
      601 ttgcgcttgg gttgcagcag caccatgcgg cccgccctaa catagccgag cgtctggtag
      661 ttggcgatga aggcgcggtc cgaggccggc ggcgagtgca ggatgtcgcg gccgaagaac
      721 ttggtgtagt agcgcaggtc gagcagcccg agcaccgtcg gcgcaatgtc gatctgcgac
      781 atgagccgat cgacgcgccg cggctgaacg tgcttcggcg cgtagacgaa cagcgggatc
      841 aggtacttgt cgaccgggat gcgcgagccg ccgcgtgcgt tcgcgccgtg gtcggcggtg
      901 atgacgaaca gcgtgtcatc gaaccagggg cggctgcgcg cgtcctgcag gaacttgccg
      961 acggcatagt cggtgtactt gaccgcccct tcgcggctgg tgcccgacgg aatatcgatg
     1021 cggtccgccg ggtaggtata gggacggtgg ttgctggtgg tcatcacgtg cacgaacaac
     1081 ggcttccttg cgtccacctt cttctcccgg tccagctcat cgagcacgta atcgaacagg
     1141 tgctcgtcgg cgactcccca tatcgtctcg tgcttcacgc gccccgaggg gatggcgcgg
     1201 cggtcgatcg agcggtagtc gttggcttcg aagaacgcgt tcatgttgtc gaaatacccg
     1261 tagccgccat aggcgaagat caccgagtag tccttgtcct cgagtacgct gccgagcgag
     1321 aacagcttgt cgttgcccgg ccggcgcacg atcgactggc cgggcgtcgg cggcaacgcg
     1381 agcgagagcg cttccatgcc gcgcaccgtg cggttgccgg tggcgtacac cctgctgaac
     1441 cagaggctct ctttcgccag gcggtcgaga ttgggcgtga tgcccttcgc attgccgtag
     1501 ctgccgagaa actcggcgcc gaggctctcg atgctcacca gcaccacgtt catgcgccgc
     1561 tcctgcccgc gcggcgccac caggcgctcg acgcccttct cctgtgcgcc cagcgccgtg
     1621 cgtaccaggg gcagcgcttc ctcgatcggc atggtggcgt agtgccggtc gtaatcgagc
     1681 tcgttgcgat agttggcggc gaagaactcg tacacgccgt tgccggccag gtcgttcggg
     1741 ttgtcgcgcg ccgagaaatt tttcgtgtcg ctgtcgaccc agcgcagcgt gccggtgaac
     1801 accgcggccc aggcgccgac ggcgaccagc gcgatgcgcc atgacaacgg cgcgccggcg
     1861 tacgaccaga tccgccggcg cagcgcgatg gcgagcacca cggcgagcgc cgccagggcg
     1921 agcagaacgc gcccgaccgg gtagctctgc cagatgttgc cgaggacttc gtgcgtatag
     1981 agcaggtagt cgacggcgat gaagttgaag cgcgagccga actcgtccca gaacagccac
     2041 tccgacaccg ccagcaggaa cgcgccgaag gccacggcgg caagcaagag aaccatgagc
     2101 gcgcgatgcg gccagctgcg cgccacccgg tccggcacca gcgccagcca aagcgccagc
     2161 ggcgctagca tgtagcaagc cgcgatcagg tcgaatgtca ggccgacgcc gaacacgcgg
     2221 atccagtcgg cgaagccgcc gacggccact tccggccgca gcgacagggc aagccgcgtc
     2281 acggcggata cgagaagcag aatcgccacc gccaggaaaa gcggcacgaa cctcggggga
     2341 attcgcttca tggaatggac tctagcacct taccctctat gcttgaggcc atgcgtcgcg
     2401 ctcgattcgc gatctgctgg ctcgtcgtcg gctatctggt tgcgacgccg ctgcgcgcgg
     2461 cggactacga ccccaacgcg aatccttttg gaccgttgcc cgatgacagc cttccgctgc
     2521 ctcgtgcgga ggacatgcct ccgctgctga cgcgcgcgtc cgaggacagc gcctctgcgc
     2581 catccttcgc cgagctggaa gcggcgggcg cgatcatcgg ccgggtcctc atccagccgc
     2641 acgacatctt cgatctcgac aacccgaagg agaacaacgc cctgttccgc ctcgccaacc
     2701 ggctgcacgt caccacccgg ccgtcggtca tcgagcgcga cgtgctgttc aagccgggcg
     2761 accgcgtctc ggtgcgcatc atcgacgaga cggagcggct gctgcgcacc aaccgctacc
     2821 tgcacgaggt gctgatccgg ccggtcaact accgcgacgg cgtggtcgac atcgaggtga
     2881 tcacgcgcga cacctggtcg ctcgaccccg gcctgagcgc ttcgcgcgcc ggcggggcga
     2941 actcgaaccg cctctctttg cgcgagcaca acttgctggg aacgggaatc tccgccggca
     3001 tctcgtactc ctcgaacgtc gatcgcgccg gaaccgaggt gggct
//
LOCUS       DASYJA010000090        11394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2465828, whole genome shotgun sequence.
ACCESSION   DASYJA010000090 DASYJA010000000
VERSION     DASYJA010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11394
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2465828"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /locus_tag="VGO02_01910"
     CDS             complement(<1..1237)
                     /locus_tag="VGO02_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEV7476472.1"
                     /translation="MKNDAKPRVYDILARAFMQEGVRTCFALLGDANMNWAARLSEQG
                     CRMVYVRHEHCALAAAMAYARKDRDVGVASVTCGPGVTQLITALPAAVRAHLPLVVFA
                     GEAPLKSGWYNQEIDQAPLITATGAAYHSLHAPERMPVATRDAFLQARRERRPVVIGV
                     PFDLQNRPWDGPADLPKTSRELLPRPSPMPPHPDDVARAALLLAGAERVVVLAGLGAV
                     EAGASAACRALAARTGGLLSTTLPARGLFHDDPFCIGISGSFTPEVGLEYLAQADLVV
                     AVGCSLAYHAGGGGQLWPKAKMLQIDIDPVAVSQGQEVARHHLRADARLGVEALTAAL
                     PARNRNWRSDEMAARIRNSRPDSHAFEVEPGLLDPRRVVEALEEALPHEWEMVNSGGH
                     CSWFFAQMPSRPQEKFLTIR"
     gene            complement(1269..1706)
                     /locus_tag="VGO02_01915"
     CDS             complement(1269..1706)
                     /locus_tag="VGO02_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV7476473.1"
                     /translation="MDPIRKAAAERAKKMGLPYAGAVLPAEAGELMKTGVKLVDVRTK
                     PELQYVGKIPGSLAVEWQTYPGGKQNPEFIGELAAAVGKDEPVMFLCRSGARSHGAAE
                     AAMRAGWKETYNVLEGFEGDKDAEGHRNTVGGWRKAGLPWVQS"
     gene            1897..3312
                     /gene="glnA"
                     /locus_tag="VGO02_01920"
     CDS             1897..3312
                     /gene="glnA"
                     /locus_tag="VGO02_01920"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEV7476474.1"
                     /translation="MAMTADEVLKMAKDKEVKFVDFRFTDTRGKEQHVQVPIKYFTKE
                     KFEAGHAFDGSSIAGWKGIQASDMLLMPDPSSARMDPFADEPMLNISCDVFEPTDGKP
                     YDRCPRGIAKRAEAFLKSSGLGDVVYFGPEPEFFIFDGVTWDVGMSGCFVKVKSEEAP
                     WSTGIDYESGNMAHRAPVKGGYFPVPPQDSLMDIRNAMCLALEAQGVDVEVHHHEVGA
                     MGQCEIGTRFAPLVQRADWMQILKYTVWNVAASYGKTATFMPKPIVGDNGSGMHCHQS
                     VWKGGKNLFAGNGYAGLSEFALYYIGGIIKHAKALNAITNPGTNSYKRLVPGFEAPIN
                     LAYSAKNRSASCRIPYVNSPNARRVEVRFPDPTANAYLAFAAMLMAGLDGVTNKIHPG
                     DPVDKNLYDLEPEEAKKVPNVCASLEEALMNLDKDHAFLTKGGVFSEDFLESYVALKH
                     EELTRFRMTTHPVEFDMYYSS"
     gene            3416..3868
                     /locus_tag="VGO02_01925"
     CDS             3416..3868
                     /locus_tag="VGO02_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEV7476475.1"
                     /translation="MTFSTLAAAASAQVTEIYKCVEKNGRPLYTSDKRETAGKKCELV
                     SREVNVVPAQKQPPPPAGAASSFPRETPEARATAKGRQRDILEKELASEQQQLDLARK
                     ELAEQESVRAGDEKNYARVLDRLQPYKDNVDLHQKNIEALKRELSNIR"
     gene            3869..4894
                     /gene="glnL"
                     /locus_tag="VGO02_01930"
     CDS             3869..4894
                     /gene="glnL"
                     /locus_tag="VGO02_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HEV7476476.1"
                     /translation="MLSFAGLDLLATAVVVLDDDFVVRYANPAAENLLTAGAKSLIGQ
                     AFLGFFAERTVLERALEEARITHWDYSAQNVTYIRGGRVEVPLSCTVTRIDAFGLGLL
                     AELRPIEQQLRQLREERLASEQQTSRELIRNLAHEIKNPLGGLRGSAQLLERELKDRE
                     LREYTQVIIKEADRLQALMDRMLTPHRTPRVEPLGIHEVLERVRALVQAEFAGSRIER
                     DYDPSLPQMSGDREQLIQAVLNIVRNAAQAGAETILLRSRALRQVTILRQRHKLALEL
                     QVVDNGPGVPDEIQDRIFNPLVSGREGGTGLGLSLAQTFVQYHQGVIEFESRPGRTTF
                     RILLPLT"
     gene            4891..6291
                     /gene="ntrC"
                     /locus_tag="VGO02_01935"
     CDS             4891..6291
                     /gene="ntrC"
                     /locus_tag="VGO02_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEV7476477.1"
                     /translation="MKPVWIVDDDRSIRWVIEKALSREGIAFSSFSSAQEALDALAGS
                     APEVLVSDVRMPGMSGLELLQTVKQRHPAVPVIVMTAYSDLDSAVAAFQGGAYEYLPK
                     PFDVDQAVELIRRALDESRREAQASEPLPQSPEMLGHAPAMQEVFRAIGRLSQSSVTV
                     LITGESGTGKELVARALHRHSARASKPFIAINTAAMPKDLLESELFGHERGSFTGAQQ
                     QRRGRFEQAEGGTLFLDEIGDMPAELQTRLLRVLSDGTFYRVGGHQQMKVNVRVIAAT
                     HQNLEQRVREGTFREDLYHRLNVIRLRLPSLRERSEDIPLLAKHFLAASARQLGVEPK
                     RLSDAALAALARLEFPGNVRQIENLCHWLTVMAAGQVIEADDLPPELREQPAGTASDW
                     VGALELEAERRLARGDIGILDALGRQFERTLIAKALARTGGRRIEAANLLGMGRNTIT
                     RKIQELGIEEDSEPET"
     gene            6372..7967
                     /gene="aceB"
                     /locus_tag="VGO02_01940"
     CDS             6372..7967
                     /gene="aceB"
                     /locus_tag="VGO02_01940"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEV7476478.1"
                     /translation="MSGPLPEGVAISGPVTRDFAQILSAEALTFLAKLHRRFEPRRQE
                     LLALRAQRQTEFDGGAFPDFLNQTKTIREADWVVAPQPKDMLDRRVEITGPTDRKMVI
                     NALNCGASTFMADFEDANCPTWHNMIDGQINLRDAVRRTISFESTGPDSTQKFYRLND
                     RSAVLIPRPRGWHLDEKHVTIDAKPVSGGIFDFALLFFHNAKELLARGSGPYFYLPKM
                     ESHLEARLWNDIFVFSQDELRIPQGSIKATALIETVVAAFEMDEILYELRSHSAGLNI
                     GRWDYIFSCIKKFRSNRDFCLADRAQVTMTAPFMRAYALLLVKTCHRRGAPAMGGMAA
                     QIPIKNDPAANQAAIEKVRQDKLREVTDGCDGTWVAHPGLVPVAKEVFDKHMPQPNQY
                     GRQRPDVKVSAKELLDFQPEKPITEAGVRNNISVGIQYLGSWLAGNGCVPVYNLMEDA
                     ATAEISRSQIWQWIRSPKGVLDDGRKVTVDLFRALLAEELAKVKAAFSDGKYDAAAQL
                     FEKLTADDRYVEFLTLPAYDLID"
     gene            complement(7964..8728)
                     /gene="xth"
                     /locus_tag="VGO02_01945"
     CDS             complement(7964..8728)
                     /gene="xth"
                     /locus_tag="VGO02_01945"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEV7476479.1"
                     /translation="MKLATWNVNSLKVRLPHLLDWLAAVQPDAICLQETKTEDANFPL
                     AAIEAAGYRAVFCGQKSYNGVAILARSQPLDVQHGIPNFPDDPKRLIAATVGDTRLVC
                     AYAPNGQSLESEKYVYKLRWYEALAAWMKDELAKRPRLALLGDLNVAPEDRDVHNPKR
                     WEGGIHVSPPERAAFRKLLEVGLADAFRLFAQPEKEFSWWDYRLNAFQRGWGLRIDFV
                     LLSAELAKGSKSALIDKGPRALERPSDHAPVIVELA"
     gene            complement(8738..9376)
                     /locus_tag="VGO02_01950"
     CDS             complement(8738..9376)
                     /locus_tag="VGO02_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEV7476480.1"
                     /translation="MIGARPFAAMLATVFAAAFTLCAASVQAQQMYRWTDEQGRTHIT
                     DTPPPASAKGVQKRGAASAKATEPQTPFGLAEAQRDFPVVLYTSPPCKQPCDDARAAL
                     NQRGVPFREVQVWDEASQEQLKKVAGNAEEVPVLTVGRSVQKGFSQDAYDALLDSARY
                     PRAGTLKPRAQAAPKVPEDYVAPENRAAVPAAEPVKPAEEPKLGPYAPRFSK"
     gene            complement(9373..>11394)
                     /locus_tag="VGO02_01955"
     CDS             complement(9373..>11394)
                     /locus_tag="VGO02_01955"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEV7476481.1"
                     /translation="LPRFSEIRPEHVTPAIDELLAAGRATVAAAERAAPTWEAFVAPQ
                     EDAAERIGRAWGQVSHLHAVLDSPALREAYNANLPKLTQYWTELGQNTALFENYKALA
                     ASREFKSLSKARQRIVENALRDFRLSGAELPADRKKRYAEISDELSKLGAKFSENLLD
                     ATNAFSLVVQEQARVAGIPPDVLQAAREAAEKEGKPGWKFTLHAPSYMPLMQYADDRA
                     LRETMYRESVTRASEFGRKEWDNTPLIARIVELRREMALLLGYPNYAEVSLEPKMAES
                     PRQVLAFLDDLAQRARPFGEHDAAELRAFARDELGIGELQAWDVAYASEKLRARRYAF
                     SDQEVKQHFPEDAVLSGLFRLVETLYGATIVPASAPVWHEDVRFFDVRDSAGGLVGQF
                     YIDLYARDTKRGGAWMDDARGRRRKGDAVQTPVAYLNCNFSRPLMSPAGGRPALFTHD
                     EVLTLFHEAGHGLHHLLTRVEDLGVSGINGVEWDAVELPSQFMENFCWEWDVLRHMTR
                     HVDTGQPLPRALFDKMIAAKNFQSGLGFLRQIEFAQFDMHLHSDFDPAAGASALELLN
                     EVRSRVAVLTQPAYNRFPNNFSHIFGGGYAAGYYSYKWAEVLSADAYGAFEEMRESRG
                     VLDAETGKRFRDEILAVGGSRPAAESFRAFRGREPSVEALLRHNGMIAA"
BASE COUNT         1932 a         3866 c         3765 g         1831 t
ORIGIN      
        1 cgcggatggt gaggaacttc tcctgcggac gcgacggcat ctgcgcgaaa aaccacgagc
       61 agtgtcctcc cgaattcacc atctcccatt catgcggcaa ggcctcttcg agcgcttcga
      121 ccacgcggcg gggatcgagc aaccccggtt cgacctcgaa ggcatggctg tcgggcctgg
      181 aattgcggat gcgcgcggcc atctcgtcac tgcgccagtt tcggttgcgg gccggcaacg
      241 ccgccgtcaa cgcctcgacg cccagccgcg cgtcggcccg caagtgatga cgcgcaactt
      301 cctggccttg actgactgcg accgggtcga tatcgatctg cagcattttc gccttcggcc
      361 agagctggcc gccgccgccc gcgtggtagg cgagcgaaca gccgaccgcg acgacgaggt
      421 cggcctgcgc caggtactcg aggccgacct cgggcgtgaa gctgcccgag atgccgatgc
      481 aaaaaggatc gtcgtggaac agccctctcg cggggagcgt cgtcgacagc agcccaccgg
      541 tcctggcggc tagcgcccgg caggccgcgc tcgcgccggc ttctaccgca cccagaccgg
      601 caagcacgac gacccgttcg gcgccggcaa gcagcagcgc ggccctggct acgtcgtcgg
      661 gatgcggcgg catcggcgaa gggcgcggca acagctcgcg cgaggtcttg ggcagatccg
      721 ccggtccgtc ccagggccgg ttctgcagat cgaacggaac accgatcacc acgggccgac
      781 gctcgcggcg cgcctgcagg aatgcgtcgc gtgtcgccac aggcatgcgt tcgggcgcgt
      841 gcaggctgtg ataggcggca cccgtcgcgg tgatgagcgg cgcctgatcg atctcctggt
      901 tgtaccaacc gcttttcagc ggcgcttcgc cggcgaacac caccagcggc agatgggcgc
      961 gcaccgccgc cggcagcgcc gtgatcaatt gcgtcacgcc gggcccgcac gtgacgctcg
     1021 ccacgcccac gtcgcggtcc tttcgcgcgt acgccatggc ggccgcgagc gcgcagtgct
     1081 cgtgcctgac atagaccatg cggcatccct gctcggacaa gcgcgcggcc cagttcatgt
     1141 tcgcatcccc gaggagcgcg aagcaggtcc gcacgccctc ctgcatgaag gccctggcaa
     1201 ggatgtcgta cacgcggggc ttggcgtcat tcttcatcgt cgcaaccccg cattgaactt
     1261 ctcaatgact agctttgcac ccagggcagg ccggccttgc gccagccgcc gaccgtgttg
     1321 cggtgcccct cggcgtcctt gtcgccctcg aagccttcga gcacgttgta agtctccttc
     1381 cagccggctc gcatcgctgc ttccgccgcg ccgtgcgagc gcgcacctga gcggcacagg
     1441 aacatcaccg gctcgtcctt gccgactgcc gccgccagct cgccgatgaa ctccggattc
     1501 tgcttgccgc ccgggtaggt ctgccattcc accgcgaggc tgccggggat cttgccgacg
     1561 tactggagct cgggcttggt ccgcacgtcc accagtttga ccccggtctt catgagctcg
     1621 cccgcttccg ccgggagcac ggcgccggcg tagggcagcc ccatcttctt cgcccgctcg
     1681 gccgccgcct tccggatcgg atccatggcg ccgcattata ggcgtgcggg gtagtgcacc
     1741 cgcttcgtgc gcagcagccc aaaacgcccc tatgcagtgc agttctgtgg gtagaatgcc
     1801 gccgaagcgc cggcagaaat tggcacggaa cgtgctgatt ggcccagcta ccactagtca
     1861 ctaccactac tacacaaacg ctcaggagac cggcagatgg caatgacggc tgatgaagta
     1921 ttgaagatgg ccaaggacaa ggaggtcaag ttcgtcgact tccgctttac cgacacccgc
     1981 ggcaaggagc agcacgtcca ggtgccgatc aaatacttca ccaaggaaaa attcgaggcc
     2041 ggccatgcct tcgacggctc gtcgatcgcc ggctggaagg gcatccaggc ctccgacatg
     2101 ctgctgatgc ccgacccgtc cagcgcgcgc atggacccgt tcgccgacga gccgatgctg
     2161 aacatcagct gcgacgtgtt cgagccgacc gacggcaagc cttatgaccg ctgcccccgc
     2221 ggcatcgcca agcgcgccga ggcgttcctc aagtcgagcg gcctgggcga cgttgtctac
     2281 ttcgggcccg agcccgagtt cttcatcttc gacggtgtca cctgggacgt gggcatgtcc
     2341 gggtgtttcg tgaaggtgaa gtcggaagaa gcgccgtggt ccaccggcat cgactatgag
     2401 tcgggcaaca tggcgcaccg cgcgccggtg aagggcggct acttcccggt gccgccgcag
     2461 gactcgctga tggacatccg caacgccatg tgcctcgcgc tcgaggcgca gggcgtcgac
     2521 gtcgaggtgc accaccacga agtcggcgca atgggccagt gcgagatcgg cacgcgcttc
     2581 gcgccgctcg tgcagcgcgc cgactggatg cagatcctca agtacacggt gtggaacgtg
     2641 gccgcgtcct acggcaagac ggcgaccttc atgcccaagc cgatcgtcgg cgacaacggc
     2701 tcgggcatgc actgccacca gtcggtgtgg aagggcggca agaacctctt cgccggcaac
     2761 ggctacgccg gcctgtccga gttcgcgctg tactacatcg gcggcatcat caagcacgcc
     2821 aaggcgctta acgcgatcac caacccgggc accaacagtt acaagcgcct ggtgccgggc
     2881 ttcgaggcgc cgatcaacct cgcgtactcg gcgaagaatc gctcggcttc ctgccgcatc
     2941 ccgtacgtca acagcccgaa cgcccggcgc gtggaagtgc gcttcccgga tccgacggcg
     3001 aacgcgtacc tcgcgttcgc ggcgatgctg atggcgggac tcgacggcgt gaccaacaag
     3061 atccatcccg gcgatccggt cgacaagaac ctgtacgacc tggagcccga agaggcgaag
     3121 aaggtgccga acgtatgcgc ctccctcgaa gaggcgctga tgaacctcga caaggaccac
     3181 gccttcctca ccaagggcgg ggtgttctcg gaggatttcc tcgagtcgta cgtggccctc
     3241 aagcacgagg agctgacacg cttccggatg accacccacc cggtcgaatt cgacatgtac
     3301 tactcgtcgt gagccggtca cgtcagactg cgttaagagt gggggcgggc gcgagcctgc
     3361 cccttctttt ttcggtagac tggacgctat gggggttctt agaccattga ttctcgtgac
     3421 atttagcacg cttgcggctg ccgccagcgc gcaggtcacg gagatctaca aatgcgtcga
     3481 gaagaacggc cgccccctgt acacgagcga caagcgcgag acggccggca agaaatgcga
     3541 gctggtgtcg cgcgaggtga acgtagtgcc cgcccagaag cagccgccac cgccggcggg
     3601 ggccgcttcc tcgttcccgc gcgagacgcc ggaggcgcgc gccaccgcca agggccgcca
     3661 gcgcgacatc ctcgagaaag agctcgcctc cgagcagcag cagctcgacc tggcgcgcaa
     3721 ggagctcgcc gagcaggagt cggtgcgcgc cggcgacgag aagaactacg cccgggtgct
     3781 ggaccggttg cagccctata aggacaacgt cgacctccac cagaagaaca tcgaggcgct
     3841 gaagcgcgag ctcagtaaca tccgctaagt gctttccttc gctggcctgg acctgctagc
     3901 cacggcggtg gtggtgctcg acgacgactt cgtcgtgcgc tacgcgaacc ccgccgcgga
     3961 aaacctgctg accgcgggcg cgaagagtct catcggccag gccttcctcg gtttcttcgc
     4021 cgagcgcacg gtcctcgagc gcgcgctcga ggaggcgcgc atcacgcact gggactattc
     4081 cgcgcagaac gtcacctaca tccgcggcgg ccgcgtcgag gtgccgctct cctgcacggt
     4141 gacgcgcatc gacgccttcg gcctgggcct gctcgccgag ctgcggccga tcgagcagca
     4201 gctgcgccag ctgcgggaag agcgcctcgc ctcggagcag cagacgagcc gcgagctgat
     4261 ccgcaacctg gcgcacgaga tcaagaaccc gctcggcggc ctgcgcggct cggcgcagct
     4321 cctcgagcgc gagctgaagg accgcgagct gcgcgaatac acccaggtga tcatcaagga
     4381 agcggaccgc ctgcaggcgc tgatggaccg catgctgacc ccgcaccgca cgccgcgcgt
     4441 ggagcccctc ggcattcacg aggtgctgga gcgggtgcgc gccctggtgc aggcggaatt
     4501 cgccggctcg cgcatcgagc gtgactacga cccgagcctg ccgcagatgt cgggcgatcg
     4561 cgagcagctc atccaggcgg tgctcaacat cgtgcgcaac gccgcccagg cgggagccga
     4621 gaccatcctc ctgcgcagcc gtgcgctgcg ccaggtcacc attctgcggc agcgccacaa
     4681 gctggcacta gaattgcagg tagtggacaa cggcccgggg gtaccggatg agatccagga
     4741 caggatcttc aatccgctgg tctccgggag ggaaggcggc accggtttgg gactatcgct
     4801 cgcacagacg ttcgtccagt accaccaggg cgtcatcgag ttcgaatccc ggccgggccg
     4861 gacaacattc aggattctgc tgccgctgac atgaaaccag tctggatcgt cgacgacgat
     4921 cgctccatcc gctgggtgat cgaaaaagcg ctcagccggg agggcatcgc gttcagctcg
     4981 ttctcgtcgg cgcaggaagc gctggacgcg cttgccggca gcgcgcccga ggtgctggtc
     5041 tccgacgtgc gcatgcccgg gatgtccggg ctcgagctgc tgcagacggt caagcagcgc
     5101 caccccgccg tgccggtgat cgtgatgacc gcgtactccg acctggatag cgccgttgcc
     5161 gctttccagg gcggggcgta cgaatatctg cccaagccct tcgatgtcga ccaggcggtc
     5221 gagttgatcc gccgcgcgct cgatgagagc cggcgcgagg cgcaagcgtc ggagccgctg
     5281 ccgcagtcgc ccgaaatgct cggccacgcg cccgccatgc aggaggtttt ccgcgctatt
     5341 ggccgtctct cgcagtcgag cgtcaccgtg ctcatcaccg gcgaatcggg cactggcaaa
     5401 gagttggttg cgcgggcgct gcaccgccac agtgcgcgcg cctcaaaacc gttcatcgcc
     5461 atcaacaccg cggcgatgcc aaaagatctg ctggagtcgg agctgttcgg ccacgagcgg
     5521 ggctcgttca ccggcgcgca gcagcagcgt cgcggccgct tcgagcaggc cgagggcggc
     5581 acgctgttcc tcgacgaaat cggcgacatg ccggcggagc tgcagacgcg cctgctgcgc
     5641 gtgctttccg acggcacttt ttaccgcgtt ggtggacacc agcagatgaa ggtcaacgtc
     5701 cgggtgatcg ccgccactca ccagaatctc gagcagcgcg tgcgcgaagg caccttccgc
     5761 gaggacctgt atcaccgcct caacgtcatc cggctgcgcc tgcccagcct gcgcgagcgc
     5821 tccgaggaca tccctctgct cgccaagcac ttcctcgccg caagcgcgcg ccagctgggc
     5881 gtcgagccca agcgtctctc cgacgcggcg ctcgccgcac tggcgcgcct cgagtttccg
     5941 ggcaacgtgc gccagatcga gaacctctgc cactggctca cggtgatggc ggccgggcag
     6001 gtaatcgaag ccgacgacct gccgccggag ctgcgcgagc agcctgcagg gacggcctcg
     6061 gactgggtcg gcgcgctgga gctggaggcc gaacggcggc tcgcccgcgg cgacatcggg
     6121 atcctggatg ccctcggccg ccagttcgag cggactctga tcgccaaggc gctggcgcgc
     6181 accgggggcc gccgcatcga ggcagccaac ctgctgggta tgggcaggaa cacaattacc
     6241 cgaaagattc aagagcttgg catcgaggaa gactcggagc cggaaaccta gcctcacccc
     6301 gcagtaagcc gatcgggccg cgtttttatg ttgcagtgcc acattggcgt acataaaatg
     6361 ctgctatgca catgtccggc ccgcttcccg aaggcgtcgc catttcggga ccggtcacgc
     6421 gcgatttcgc gcagatcctc agtgccgaag cgctgacctt cctcgccaag cttcaccggc
     6481 gcttcgagcc gcgccgccag gagctgctgg cactgcgcgc gcaacggcaa acggagttcg
     6541 acggcggcgc atttcctgat tttttaaatc aaactaaaac cataagagaa gccgactggg
     6601 tcgtcgcgcc gcagccaaag gacatgctcg accgccgcgt cgagatcacc ggcccgaccg
     6661 accgcaagat ggtgatcaac gcgctcaact gcggcgcgtc caccttcatg gcggacttcg
     6721 aggacgccaa ctgcccgacc tggcacaaca tgatcgacgg gcagatcaac ctgcgcgacg
     6781 ccgtgcgccg caccatcagc ttcgagagca ccggacccga cagcacgcag aagttttacc
     6841 gcctgaacga caggagcgcg gtgctcatcc cgcggccgcg cggctggcac ctcgacgaga
     6901 agcacgtcac catcgacgcc aagccggtgt ccggcggcat cttcgatttc gcgctgctct
     6961 ttttccacaa cgcgaaggag ctgctggcgc gcggctcggg gccgtacttc tacctgccga
     7021 agatggagtc ccatctcgag gcgcggctgt ggaacgacat tttcgtcttc tcgcaggacg
     7081 agctgaggat cccgcagggc tcgatcaagg ccaccgcgct gatcgagacg gtggtggccg
     7141 cgttcgagat ggacgagatc ctgtacgagc tgcgcagcca ttcggccggc ctcaacatcg
     7201 gccgctggga ctacatcttt tcctgcatca agaagttccg ctccaaccgc gacttctgcc
     7261 tcgccgaccg cgcgcaggtg acgatgaccg cacccttcat gcgcgcctac gccctgctgc
     7321 tggtcaagac ctgccaccgg cgcggcgcgc cggcgatggg cgggatggcg gcgcagatcc
     7381 cgatcaagaa cgacccggcg gcgaaccagg cggcgatcga gaaggtgcgc caggacaagc
     7441 tgcgcgaggt caccgacggc tgcgacggca cctgggtggc gcacccgggc ctcgtgccgg
     7501 tggcgaagga agtgttcgac aagcacatgc cgcagccgaa ccagtacggc cggcagcgcc
     7561 ccgacgtgaa ggtgagcgcg aaggagctgc tcgacttcca gcccgagaag ccgatcaccg
     7621 aagccggcgt gcgcaacaac atctccgtcg gcatccagta cctcggctcc tggctcgccg
     7681 gcaacggctg cgtgccggtg tacaacctga tggaggatgc cgccaccgcc gagatctcgc
     7741 gctcgcagat ctggcagtgg atccgctcgc cgaaaggcgt gctggatgat ggccgcaaag
     7801 taaccgtgga tctattcaga gcattacttg ctgaagagct cgccaaggtg aaggccgcct
     7861 tcagcgacgg caagtacgac gcagcggcgc agctcttcga gaagctgacc gccgacgatc
     7921 gttacgtcga gttcctcacg ctcccggcgt acgacctgat cgactaggcg agctcgacga
     7981 tcaccggggc gtgatcggag ggccgctcca gggcgcgcgg ccccttgtcg atgagcgccg
     8041 acttgcttcc cttggcgagc tccgccgaca gcagcacgaa atcgatgcgc agcccccagc
     8101 cgcgctggaa tgcattcagg cggtagtccc accacgagaa ttccttctcc ggctgcgcaa
     8161 acaggcggaa cgcatcggcg agaccgacct ccagcagctt acggaacgcg gcgcgctccg
     8221 gcggcgagac gtggatgcca ccttcccagc gcttcgggtt gtgcacgtcg cggtcctcgg
     8281 gagcgacgtt gaggtcgccg agcagcgcca gccgcggccg cttcgccagc tcgtccttca
     8341 tccatgcggc cagcgcctca taccagcgca gcttgtagac gtacttttcc gattcgagcg
     8401 attgtccgtt cggcgcgtag gcgcagacca ggcgcgtgtc gccgaccgtg gcggcaatca
     8461 ggcgcttcgg gtcgtcgggg aagttcggga tgccgtgctg cacgtcgagc ggttgcgagc
     8521 gcgcgaggat cgcgacgccg ttgtacgact tctgcccgca aaacaccgcg cggtagccgg
     8581 ccgcctcgat cgcggcgagc gggaagttcg cgtcctcggt cttggtttcc tgcaggcaga
     8641 tcgcgtcggg ctgcacggcg gccagccagt cgagcaggtg cggcagccgc accttgagcg
     8701 agttgacgtt ccaggtggcg agcttcaccc cggacgatta cttcgagaag cgcggggcgt
     8761 acgggcctag cttgggttcc tcggccggct tcaccggctc ggcggccggg accgcagcgc
     8821 ggttctcggg cgccacgtag tcttccggca ccttgggtgc cgcctgcgcg cgcggcttga
     8881 gcgtgcccgc acgcggatag cgggcggagt cgagcagcgc atcgtacgcg tcctgcgaga
     8941 agcccttctg cacgctgcgg cccacggtga gcaccggcac ttcttcggca ttgccggcga
     9001 ccttcttcag ctgctcctgg ctcgcctcgt cccagacctg cacttcgcgg aacggcaccc
     9061 cgcgctggtt gagcgcggcg cgcgcatcgt cgcacggctg cttgcacggc ggggaggtgt
     9121 agaggacgac cggaaaatcg cgttgcgcct ccgccagccc gaagggcgtc tgcggctccg
     9181 tggccttcgc cgaagcggca cccctcttct gcacgccttt ggcggaggcg ggcggggggg
     9241 tgtcggtgat atgggtgcgg ccctgctcgt cggtccagcg gtacatctgc tgcgcctgga
     9301 ccgaggcagc gcacagtgtg aaagctgcgg cgaatactgt ggcgagcatc gcggcgaacg
     9361 ggcgtgcgcc gatcatgcgg cgatcatacc attgtgccgc agcagcgctt cgacgctcgg
     9421 ctcgcggccg cggaaggcgc ggaacgattc cgctgccgga cggctgccgc cgacggcgag
     9481 gatttcgtca cggaagcgct tgccggtctc ggcatcgagc acgcccctcg actcgcgcat
     9541 ctcttcgaac gcgccgtagg cgtcggccga gagcacctcg gcccatttgt agctgtagta
     9601 gccggcggca tagccgccgc cgaagatgtg cgagaaattg ttcgggaagc ggttgtaggc
     9661 gggctgcgtc agcaccgcga cgcggctgcg cacttcgttc agcagctcga gcgcgctcgc
     9721 gcccgccgcg gggtcgaagt cggagtgcag gtgcatgtcg aactgcgcga actcgatctg
     9781 ccgcaggaag cccaggccgc tctggaagtt cttcgccgcg atcatcttgt cgaacagggc
     9841 gcgcggcagg ggctgccccg tgtccacgtg ccgcgtcatg tggcgcagca cgtcccattc
     9901 ccagcagaaa ttctccatga actggctggg cagctcgacc gcatcccact cgacgccgtt
     9961 gatgccggag acgccgaggt cctcgacgcg cgtcagcagg tggtgcaggc cgtgcccggc
    10021 ctcgtggaac agcgtgagca cctcgtcgtg cgtgaacagg gccggcctgc cgcccgcggg
    10081 tgacatgagg ggccgggaga aattgcagtt caggtaggcg accggtgtct gcaccgcatc
    10141 gcccttgcgg cgcctgccgc gcgcatcgtc catccacgcg ccgccgcgct tggtgtcgcg
    10201 cgcgtagaga tcgatataga actggcccac caggccgccg gcggagtcgc gcacgtcgaa
    10261 gaagcgcacg tcctcgtgcc acaccggcgc gctggcgggt acgatcgtcg cgccgtagag
    10321 cgtttcgacc aggcggaaca ggccggacag caccgcgtcc tcggggaagt gctgcttcac
    10381 ctcctggtcg gaaaaggcgt agcgcctggc gcgcagcttc tccgatgcat aggcgacgtc
    10441 ccacgcctgc agctcgccga tgccgagctc gtcgcgcgcg aaggcccgca gctcggccgc
    10501 gtcgtgctcg ccgaagggcc gcgcgcgctg cgccaggtcg tcgaggaagg cgagcacctg
    10561 gcgcggcgac tcggccatct tgggctcgag cgaaacctcg gcgtagttcg ggtagccgag
    10621 cagcagcgcc atctcgcggc gcagctcgac gatgcgcgcg atgagcggcg tgttgtccca
    10681 ttccttgcgg ccgaactcgg aggcgcgcgt gacgctctcc cggtacatgg tctcgcgcag
    10741 cgcgcgatcg tcggcgtact gcatcagcgg catgtaggac ggcgcatgca gcgtgaattt
    10801 ccagcctggc ttgccttcct tctcggcggc ttcgcgcgcc gcctgcagga cgtccggcgg
    10861 gattccggcg actcttgcct gctcctgcac gacaagggaa aaagcgttcg tggcgtcgag
    10921 caggttctcg gagaacttgg cgccgagctt ggacagctca tcggagatct cggcgtagcg
    10981 ctttttcctg tcggcgggca gctcggcgcc gctcaggcgg aagtcgcgca aggcattctc
    11041 gacgatgcgc tgccttgctt ttgaaagaga tttgaattcc cgggatgcgg caagcgcctt
    11101 gtagttctcg aacagcgccg tgttctgccc gagctcggtc cagtactggg tgagcttcgg
    11161 caggttggcg ttgtaggcct cgcgcagcgc agggctgtcc agcacggcgt gcaggtgcga
    11221 cacctggccc caggcacggc cgatgcgctc ggctgcgtcc tcctgcggcg cgacgaacgc
    11281 ctcccaggtc ggcgcggccc gttcggcggc ggcgacggtg gcgcgccccg cggcgagcag
    11341 ctcgtcgatc gcgggggtga cgtgctccgg cctgatctcg ctgaaacggg gcag
//
LOCUS       DASYJA010000091         3310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1105375, whole genome shotgun sequence.
ACCESSION   DASYJA010000091 DASYJA010000000
VERSION     DASYJA010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105375"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..380)
                     /locus_tag="VGO02_01960"
     CDS             complement(180..380)
                     /locus_tag="VGO02_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476482.1"
                     /translation="MNVVRLDEYRKSRLPARPQAASAPDAEHYFCMRCDSDQFRLYAG
                     GEVHCAKCGSLMQNLSIGETGR"
     gene            complement(407..811)
                     /locus_tag="VGO02_01965"
     CDS             complement(407..811)
                     /locus_tag="VGO02_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476483.1"
                     /translation="MTRFALAVFLGIFLLTGIAQEKYRVVFQVSDNDPAKWNLALNNA
                     RNVQADLGKANVQVEIVAYGPGLAMLKAESPVSARLAEALDGNVGLLACVNTMENTKT
                     TKSDMYGGIGYVQAGVTHIMKRQKEGWAYIRP"
     gene            complement(814..1821)
                     /locus_tag="VGO02_01970"
     CDS             complement(814..1821)
                     /locus_tag="VGO02_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEV7476484.1"
                     /translation="MQELGRVGGHRRVDFRGDDEFPPAQLSQQVAHRKIISSGFIPLP
                     AFRLPELPDIAVYLDSLERRILGARLLRVRLLSPFLLRTAVPPIASAEGRKLLALRRL
                     GKRIVLALEGDQFLVIHLMIAGRLQWDSKRRGLAVFEFDTGNLTLTEAGTKRRASLHL
                     VQGEAALAAMDPGGIDVFSADFQTFRSRLQSGNHTVKRALSDPHMFSGIGNAYSDEIL
                     HRAKMSPFAMTSQLSEDDIRRLHDATKETLNLWTERLRRQTGEGFPEKVTAFRDEMAV
                     HGRYGKPCPVCGAPVQRIVYAENETNYCARCQTGGKILADRALSRLLKSSWPRNIDEL
                     E"
     gene            1792..2655
                     /locus_tag="VGO02_01975"
     CDS             1792..2655
                     /locus_tag="VGO02_01975"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEV7476485.1"
                     /translation="MSTDPAEFLQRAMPLKGLATAVNVTDGAGAKVHLSSLAAAHFLV
                     QAGIEPIFQMTCRDRNRLALQGDLIGAAALGIHNILVLAGDDPKLGDQPEAKAVFDLN
                     SRELLAMAARMRAEHKLPTGVEIKGKLDLVLGAADVPVDPPADWNPKGLLGKAEAGAD
                     FVQTQFCMDAGVVRRYAARLLDLGFRLPILIGVAPIPSARSARWMREKLFGSLIPDAT
                     VERLEKAADPKEEGARICIELLQQLVEIPGVAGAHVMAPQNFSQIPRVIADSGVTRRK
                     KTWNSPKALSS"
     gene            2625..>3310
                     /locus_tag="VGO02_01980"
     CDS             2625..>3310
                     /locus_tag="VGO02_01980"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7476486.1"
                     /translation="MEFAKSFVELSGCRTHVRRAGKGQTLLFLHGASGMPAILPCLQK
                     LAERFDVVVPEHPGYGQSDEPEWLENIHDMAYFYLDFLEKLDLKDVLLVGNSMGGWIA
                     LEIAVRSTARIRSMVVVSPAGVKAPGVLPADTFLMSPEEQVRALVFDQKLADARLAEP
                     MTPEALDTALKNRHTTARLAWEPRFHDPHLPKWLHRIKVPVTIVWGEQDKILPVGFAY
                     ELKKLIKNSK"
BASE COUNT          560 a         1074 c         1118 g          558 t
ORIGIN      
        1 gcaggcccag cggatgttgg actactggag cggcgtgtgg cgcgctgccg gcgacaacca
       61 gatggccgcg gcacggctcg cggcgtcgca agtgcaggcg gtgagcggcg gcctgcgcga
      121 cacggcgatg aatcaggaaa agcggccgga gccgcagcgc aagagcgcct agcggccgct
      181 catctgcccg tttcgccgat gctgaggttc tgcatcagcg agccgcactt ggcgcagtgc
      241 acctcgccgc cggcgtacag gcggaactgg tcgctgtcgc agcgcatgca gaagtagtgc
      301 tcggcgtcgg gcgccgaggc ggcctgcggc cgcgccggca ggcgggactt gcggtactca
      361 tccaggcgca cgacgttcat cgccgtccac cctacggcat cccgggttac ggccgtatat
      421 aggcccaacc ctccttctgg cgcttcatga tgtgcgtcac cccggcctgc acgtagccga
      481 ttccgccgta catgtcggat ttcgtcgtct tggtgttctc catggtgttc acgcaggcga
      541 gcaggccgac attgccgtcg agcgcctcgg cgaggcgcgc cgaaaccggc gactcggcct
      601 tgagcatggc gagccccggg ccataggcga cgatctcgac ctgcacgttc gccttgccga
      661 ggtcggcctg cacgttgcgg gcgttgttca gcgccaggtt ccatttcgcc gggtcgttgt
      721 cgctcacctg gaagaccacc ctgtatttct cctgcgcgat tcccgtcagc aggaagatgc
      781 cgaggaaaac agccaaagca aacctggtca tgttcattcc aattcgtcga tgttgcgggg
      841 ccagctgctt ttgagcagcc gcgacagcgc gcggtcggcg aggatcttac cgccggtctg
      901 gcagcgcgcg cagtagttcg tttcattctc ggcatagacg atgcgctgca ccggcgcgcc
      961 gcacaccggg cagggcttgc cgtaccgccc gtgcaccgcc atctcgtcgc ggaacgccgt
     1021 caccttttcc ggaaagcctt cccctgtttg gcgccgcagc ctctcggtcc acaggttcag
     1081 ggtttccttg gtcgcatcgt gcagcctgcg gatatcgtcc tcggacagct gggaggtcat
     1141 ggcgaagggg gacatctttg cgcggtggag tatctcgtcc gagtaggcgt ttccgatacc
     1201 cgaaaacata tgaggatcgc tcaatgcccg cttcaccgtg tggttcccgg attgaagccg
     1261 cgatctgaac gtctggaagt ccgcagaaaa aacgtcgatt cctccgggat ccatagcggc
     1321 gagggctgcc tcaccttgca caagatgcag cgaagcgcgg cgcttggtac cggcctcggt
     1381 caaggtgagg ttgccggtgt cgaactcgaa taccgcgagg ccccgccgct tgctgtccca
     1441 ctgcagtcgg ccggcgatca tgaggtggat caccagaaac tggtcgccct cgagcgcgag
     1501 cacgatgcgc ttgccgaggc ggcgcagagc gaggagcttc ctgccctcgg cgctggcgat
     1561 cggtggcacg gcggtgcgca gcaggaacgg gctcagcagc cgcacgcgca gcagccgggc
     1621 gccgaggatg cggcgctcga ggctgtcgag gtagacggcg atgtcgggaa gctccggcaa
     1681 gcgaaacgcg gggagtggga tgaatcccga tgatatgatt tttcggtgag cgacttgctg
     1741 agaaagctgc gcggggggga attcgtcgtc accgcggaaa tcaacccgcc ggtgtccacc
     1801 gacccggccg agttcctgca acgcgcgatg ccgctcaagg ggctggcgac cgcggtcaac
     1861 gtcaccgacg gcgccggcgc gaaggtgcat ctctccagcc tcgccgccgc gcatttcctg
     1921 gtccaggccg ggatcgagcc gatcttccag atgacctgcc gcgaccgcaa ccggctcgcg
     1981 ttgcagggcg acctcatcgg cgccgccgcg ctgggcatcc acaacatcct ggtgctcgcc
     2041 ggcgatgatc ccaagctggg cgaccagccc gaggcgaaag cagtgttcga cctcaacagc
     2101 cgcgagctgc tggcaatggc ggcgcgcatg cgcgccgagc acaagcttcc caccggcgtc
     2161 gagatcaagg gaaagctcga cctggtcctc ggcgcggccg acgtgccggt cgacccgccg
     2221 gccgactgga acccgaaggg cctgctcggc aaggccgagg cgggtgcgga tttcgtgcag
     2281 acgcagttct gcatggacgc gggcgtggtg cggcgctacg cggcgcggct gctcgatctg
     2341 ggcttccggc tgccgatcct gatcggcgtg gcgccgatcc cgtcggcgcg ctcggcgcgc
     2401 tggatgcgcg agaagctgtt cggcagcctg atccccgacg ccaccgtcga gcgcctggag
     2461 aaggcggccg acccgaagga ggaaggcgcg aggatctgca tcgagctgct gcagcagctg
     2521 gtcgagatcc ccggcgtcgc cggcgcgcac gtgatggcgc cgcagaactt ttcccagatt
     2581 ccgcgggtca tcgccgattc gggcgtcacc cgcaggaaga agacgtggaa ttcgccaaaa
     2641 gctttgtcga gctgagcggc tgccgcacgc acgtgcgccg cgcgggaaag gggcagaccc
     2701 tgctcttcct gcacggcgcc agcggcatgc cggcgatcct gccttgcctg caaaagctcg
     2761 cggagcgctt cgacgtcgtg gtgccggagc atccaggcta cggccagtcg gacgagccgg
     2821 aatggctcga gaacatccac gacatggcct acttctacct ggacttcctc gagaagctcg
     2881 acctcaagga cgtgctgctc gtcggcaact ccatgggcgg ctggatcgcg ctcgagatcg
     2941 ccgtgcgcag caccgcgcgc atcaggtcga tggtggtggt cagcccggcg ggagtgaagg
     3001 cgccgggcgt gctgccggcc gacaccttcc tcatgtctcc tgaggagcag gtgagagccc
     3061 tggtgttcga ccagaagctg gcggacgcgc gccttgccga gccgatgacg cccgaggcgc
     3121 tggataccgc gctcaagaac cggcacacca ccgcgcgcct cgcctgggag ccgcgcttcc
     3181 acgatccgca cctgccgaag tggctgcatc gcatcaaggt gccggtgacc atcgtgtggg
     3241 gcgagcagga caagattctt cctgttggtt ttgcatacga attgaaaaag ctgatcaaga
     3301 attcaaaatt
//
LOCUS       DASYJA010000092         4378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_742310, whole genome shotgun sequence.
ACCESSION   DASYJA010000092 DASYJA010000000
VERSION     DASYJA010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742310"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..651
                     /locus_tag="VGO02_01985"
     CDS             1..651
                     /locus_tag="VGO02_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV7476487.1"
                     /translation="MLPFLEPVTLPLGTAVYESGAAQGYVYFPTSSIVSLLYVLADGA
                     SAEIAITGSEGLVGIALFMGGETTPSRAVVQSAGHGYRLRASVLKTEFERGGALQHLL
                     LRFTQALITQMTQTAVCNRHHAVHQQLCRWLLLSLDRLESNELVMTQELIANMLGVRR
                     EGVTEAAGRLQADGLIEYSRGRITILDRARLEAQVCECYSVVRKEYDRLLPATLTS"
     gene            719..1417
                     /locus_tag="VGO02_01990"
     CDS             719..1417
                     /locus_tag="VGO02_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV7476488.1"
                     /translation="MKDIPNSLLAALPRKSYLKLLPALAPVDLIFGEVLYEAGAPIQD
                     VYFPGRSLVSLLTVVEGHLALEVGLVGREGMVGFPLALGVGVSPVRALVQGGGPAMKM
                     SAARFRTELRASPPLQRELNRYVYTMMAQISQTAACNRFHMVEARLARWLLMTRDRVR
                     SSDFRMTHEFLSHMLGVRRVGVTEAASALQRRKLIEYRRGNIRILDDRGLEASCCSCY
                     KVVRDMHDGKPGLA"
     gene            complement(1443..1814)
                     /locus_tag="VGO02_01995"
     CDS             complement(1443..1814)
                     /locus_tag="VGO02_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476489.1"
                     /translation="MTRYAIPFALTALALVAACAHRADPAPAPVVVVPQQPPATVVAA
                     PSVPMVAVQPTALRAGYGRIESISAVPTAAGAGSTSPGSVRRFGIKMEDGTVQYVDSD
                     APSSIGERIQLTGDGYIRPAP"
     gene            complement(1870..2748)
                     /locus_tag="VGO02_02000"
     CDS             complement(1870..2748)
                     /locus_tag="VGO02_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEV7476490.1"
                     /translation="MRIVLPFGPGGVADITTRTLAPRMSDSLGQQVIVENAPSAGGIR
                     ASETVARADPDGHTLLLLTNGNAVSQALFKSLPYDPMNDFSMISTVGFFSMVFVTRGD
                     SPFKSMKDVLATAKAKPGTLNIGTISPGGTQHLAGELFRSTAGIDAVVIPHKTTGEVV
                     VGVRNGSLDMAVDFIAPLISGIQSGALRPLAVSSGKRFPGLADVPTAIEAGVAGYDVA
                     SWNALAAPAKTPRGAIDRVREALIKALANPDVQKRFAELGVEGRSSTPEQLRDFFVSE
                     SARWTRVVEAAKIPKQ"
     gene            complement(2817..3806)
                     /locus_tag="VGO02_02005"
     CDS             complement(2817..3806)
                     /locus_tag="VGO02_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7476491.1"
                     /translation="MIVDCHGHYTTAPQPLWDWRKDQVGNKKPDAKGPKISDDQIRES
                     LEGAQLKLQRERGSDITFFSPRAGSMEHHVGDAAISAQWSRVQNDLIHRVCTLYPKNF
                     APVCMLPQSPGVSPANCVEELERCVEEMGFIGCNLNPDPSGGWWKEVPLTDKWWYPVY
                     EKMSELDVPAMVHVSSSCNPAFHFTGAHYINADTTAFMQLIQGDLFKDFPTLKFVIPH
                     GGGAVPYHWGRYRGLAQDMKKPLLKEHLLKNVYFDTCVYHQPGIDLLLKVIPVENILF
                     ASEMVGAVRGIDPETGQFFDDTKRYIDAGKLSAGDKQRIFEGNAKKVYSRWRI"
     gene            complement(3803..>4378)
                     /locus_tag="VGO02_02010"
     CDS             complement(3803..>4378)
                     /locus_tag="VGO02_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV7476492.1"
                     /translation="AYNPESGPLRPHDLNGKRVGMRAYSVTTATWVRGILADDYGVDL
                     DKVNWITFEDPHVAEFRDPPNATRMPAGKDPTAMLLAGEIDAAILAEPPKDGPLKTLI
                     PDPAAAAAAWKAKNRAIQINHMVTLKNTVSKEEEKKIYELLVESRKAAGNPEMNPFGL
                     EENRRNLEVAMDCMVRQRMLPRRFTVEELLR"
BASE COUNT          677 a         1440 c         1477 g          784 t
ORIGIN      
        1 ctgctgccct ttctcgagcc cgtgactttg ccgctcggca cggcggtcta tgaatccggg
       61 gccgcccagg gctacgtcta cttcccgacc agcagcatcg tgtcgctcct ttacgtactg
      121 gcggacggtg cttcggcgga gatcgccatc accggatccg agggcctggt gggcattgcg
      181 ctattcatgg gcggcgagac cacgccgagc cgcgcggtcg tgcaaagcgc cggccacggc
      241 taccggctca gggcctccgt gctgaagacc gagttcgagc gcggcggcgc gctgcagcac
      301 ctgctgctgc gcttcacgca ggcgctgatc acgcagatga cgcagaccgc ggtctgcaac
      361 cgccaccacg cggtccacca gcagctgtgc cgctggctgc tcttgagcct ggaccggctc
      421 gagtcgaacg agctggtgat gacccaggag ctgatagcca acatgctcgg cgtgcgccgc
      481 gaaggcgtta ccgaggccgc cggcaggctg caggccgatg gcttgatcga gtacagccgg
      541 ggccgcatca ccatcctcga ccgggcgcgg ctcgaagcgc aggtgtgcga gtgctactcg
      601 gtggtcagga aggaatacga ccggctgctc ccggcgacct tgacgtcgta gaccttcctg
      661 tcgcgctcag tgcgcgagcg cacatacggc gcgctgccag cgcggacaat tgagcgggat
      721 gaaggatatc ccgaacagct tgcttgccgc gctgccgcgc aagtcctacc tgaagctgct
      781 ccccgccctc gcgcccgtcg atctgatctt cggcgaggtg ctctacgagg ccggcgcacc
      841 gatccaggac gtctactttc ccggccgctc gctggtttcg ctgctcacgg tagtggaagg
      901 ccacctcgcg ctcgaggtcg ggctggtggg ccgcgagggc atggtcggtt tcccgctcgc
      961 gctcggcgtc ggcgtctcgc cggtgcgcgc cctcgtgcag ggcggcgggc cggcgatgaa
     1021 aatgagtgcc gcccgcttcc gcaccgagct gcgcgccagc ccgccgctac agcgcgagct
     1081 gaaccgctat gtgtacacga tgatggcgca gatctcgcaa accgccgcct gcaaccgctt
     1141 ccatatggtg gaggcgcggc tcgcccgctg gctgctgatg acccgcgatc gcgtgcgttc
     1201 cagcgatttc cgcatgacgc acgagtttct ctcgcacatg ctcggcgtgc gccgggtcgg
     1261 cgtcaccgaa gcagcttcgg ccctgcagcg gaggaagctg atcgaatacc gccgcggcaa
     1321 catcaggatc ctggatgacc gcggtctcga agcgagctgc tgcagctgct acaaggtggt
     1381 cagggacatg cacgacggca agcccggcct ggcatagcca tgccggacct gctgcagtgc
     1441 ggttacggcg cggggcggat gtagccgtcg ccggtcagct ggatgcgctc gccgatggag
     1501 ctcggcgcat cgctgtcaac gtactgcacc gtgccgtcct ccatcttgat accgaagcgc
     1561 cgcacggagc cgggcgaggt cgagccggcg cctgctgcgg tcggcaccgc gctgatcgac
     1621 tcgatacgac cgtagcctgc gcgcagcgca gtgggctgca ccgcgaccat cggcaccgag
     1681 ggcgccgcga cgacggtggc cgggggttgt tgcggaacga cgacgaccgg cgcgggcgcc
     1741 ggatcggcgc ggtgcgcgca ggcggcgacc agcgctaagg ccgttagcgc aaacgggatg
     1801 gcatatctgg tcatgagaaa tctcctgtag gtagacaccc acagagagca atccgcgttc
     1861 ccgcaagggt tactgcttcg ggatcttcgc cgcctccacc acccgcgtcc agcgggccga
     1921 ctcgctgacg aagaagtcgc gcagctgctc cggggtactc gagcggcctt cgacgccgag
     1981 ctccgcaaag cgtttctgca catccgggtt ggccagcgcc ttgatcagcg cctcgcgcac
     2041 gcggtcgatg gcgccgcgcg gcgtcttcgc cggcgcggcg agcgcgttcc acgaggcgac
     2101 gtcgtagccc gcaacgccgg cctcgatcgc ggtcggcacg tcggccaggc cggggaagcg
     2161 cttgcccgag ctcaccgcga gggggcgcag cgcgccggac tggatgcccg agatgagcgg
     2221 cgcgatgaag tccaccgcca tgtcgaggct gccgttgcgc acgccgacga ccacctcgcc
     2281 ggtagtcttg tgcgggatca ccaccgcgtc gatgccggcg gtcgagcgga acagctcgcc
     2341 ggcgaggtgc tgcgtgccgc ccgggctgat ggtgccgatg ttgagcgtgc cgggcttcgc
     2401 cttggccgtc gcgagcacgt ccttcatcga cttgaacggc gagtcgccgc gcgtcacgaa
     2461 caccatgctg aagaagccga ccgtggagat catggaaaag tcgttcatcg ggtcgtaggg
     2521 cagcgacttg aacagcgcct ggctgaccgc gttgccgttg gtcaacagga gcagcgtgtg
     2581 gccgtccgga tcggcgcgcg ccaccgtctc ggaggcgcgg atgccgccgg cgctcggcgc
     2641 gttttcgacg atcacctgtt gcccgaggct atcgctcatg cgcggcgcga gggtgcgcgt
     2701 ggtgatgtcg gcgaccccgc ccggcccgaa ggggagcacg atgcgcacag gttttgacgg
     2761 gtagctctgc gaaccaaccc accccggaaa aagcaaaacc agaatcagca gcgttctcat
     2821 atcctccacc ttgaatagac ttttttggcg ttgccttcga atatcctctg cttgtcgccg
     2881 gcgctgagct tgcccgcgtc gatgtagcgt ttcgtatcgt cgaaaaactg tcccgtctcc
     2941 gggtcgatgc cgcgcaccgc gcccaccatc tcggaggcga acaggatgtt ctccaccggg
     3001 atcaccttca gcagcaggtc gatgcccggc tggtggtaga cgcaggtgtc gaagtacacg
     3061 ttcttcagca ggtgctcctt gagcagcggc ttcttcatgt cctgcgccag cccccggtag
     3121 cgcccccagt ggtacggcac ggcgccgccg ccatgcggga tgacgaactt cagcgtcggg
     3181 aagtccttga acagatcgcc ctggatgagc tgcatgaacg cggtggtgtc ggcgttgatg
     3241 tagtgcgcgc cggtgaagtg gaacgccggg ttgcacgaag agctgacgtg caccatggcc
     3301 ggcacgtcga gctcggacat tttttcgtac accgggtacc accacttgtc ggtgagcggc
     3361 acttccttcc accagccgcc ggaggggtcg gggttcaggt tgcagccgat gaagcccatc
     3421 tcctcgacgc agcgctcgag ctcctcgaca caattggcgg gcgagacgcc cggcgactga
     3481 ggcagcatgc agaccggggc gaagttcttc gggtagagcg tgcagacgcg gtggatcagg
     3541 tcgttctgca cccgcgacca ctgcgcgctg atggcggcgt cgccgacgtg gtgctccatc
     3601 gagccggcgc gcggcgagaa gaaggtgatg tcgctgcccc gctcccgctg cagcttcaat
     3661 tgggcgcctt ccaggctttc gcggatctgg tcgtccgaga tcttcggacc ttttgcatcg
     3721 ggcttcttgt tgcctacctg gtccttgcgc cagtcccaaa ggggctgcgg cgcggtggtg
     3781 tagtggccgt ggcagtcgac gatcatcgga gcagctcctc caccgtgaag cgccgcggca
     3841 gcatccgctg gcggaccatg caatccatcg cgacttccag gttgcggcga ttctcttcca
     3901 ggccgaacgg attcatctcg ggattgcccg ccgcttttct gctctctaca agcagctcgt
     3961 agatcttctt ttcttcttct tttgaaactg tgtttttcaa ggtcaccatg tggttgatct
     4021 ggatggcgcg gttcttcgcc ttccacgccg cggccgccgc cgccgggtcc gggatcagag
     4081 ttttcagcgg accgtccttc ggcggctcgg cgaggatcgc ggcgtcgatc tcgccggcga
     4141 ggagcatcgc cgtcggatcc ttgcccgccg gcatgcgcgt ggcgttgggc gggtcgcgga
     4201 attccgcgac gtgcgggtcc tcgaaggtga tccagttcac cttgtcgagg tcgacgccgt
     4261 agtcgtcggc caggatgccg cgcacccagg tggcggtggt gaccgaatag gcgcgcatgc
     4321 cgacgcgctt gccattgagg tcgtgcggcc gcagcggccc gctctccgga ttgtaggc
//
LOCUS       DASYJA010000093         1994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2012505, whole genome shotgun sequence.
ACCESSION   DASYJA010000093 DASYJA010000000
VERSION     DASYJA010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2012505"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..722)
                     /locus_tag="VGO02_02015"
     CDS             complement(84..722)
                     /locus_tag="VGO02_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEV7476493.1"
                     /translation="MKLRLRISMSLDGFAAGPQQSVDNPLGVGGMRLHEWVFPLKAWR
                     EMHGMEGGEVNESSAVVQESLANIGATIMGRNMFGGHPGGWDAKQPWNGWWGDNPPFH
                     HPVFVLTHHARQPLRMQGGTAFHFVTQGIEVALDQAKRAAGGKDISLAGGARAAQQYL
                     KAGLVDEMEINLAPTLLGSGERLFDGVTDLNGLELVRTVAAPKVTHLKFARR"
     gene            complement(724..1176)
                     /locus_tag="VGO02_02020"
     CDS             complement(724..1176)
                     /locus_tag="VGO02_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7476494.1"
                     /translation="MEVTVRSAAARDEKRWRELFDGYCIFYKRTPSEALTRHTWARIL
                     DPASPVHAIVADHEKHGVIGIANYVVHEHTLGLKPVCYLGDLFVDPARRAGGIGEQLI
                     AWLRDEMKKEGWPRLYWHTRLNNYRARGLYDKFTPHSDFVRYTIYNEG"
     gene            complement(1176..>1994)
                     /locus_tag="VGO02_02025"
     CDS             complement(1176..>1994)
                     /locus_tag="VGO02_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV7476495.1"
                     /translation="LFACAFNLLVGFGGLLSFGHAMFLGTAGYAAAHSARMWGFPPEA
                     AILFGTFCAALLGFVTGLLAIRRQGIYFAMITLALAQMIYFFYLQAPFTHGEDGIQSV
                     PRGRLFGFIDLTSIWAMYGTVLVIFAAGFLVVFRTIHSPFGQVLKAIRENEPRAISLG
                     YDTTRYKFLAFLLSATLAGLAGATKAIVFQLASLTDAHWSMSGEVVLITLLGGMGTVF
                     GPVAGALVVISMENYLAQFGAWVTIAQGVIFVVCVLTFRRGIVGEIAHFLKKPL"
BASE COUNT          349 a          642 c          689 g          314 t
ORIGIN      
        1 ggatgacaac cggagggaac ttacccgtcg atagtcaacc gccttgcgag acgccacact
       61 agctgtatcg cctacctgcc cgctcagcgc cttgcgaact tgaggtgcgt gaccttgggg
      121 gcggcgaccg tgcgcaccag ctcgagaccg ttcaggtccg tgacgccgtc gaacaggcgc
      181 tcgccgctgc cgagcagcgt cggcgcgagg ttgatctcca tctcgtcgac cagcccggcc
      241 ttcaggtact gctgcgctgc cctcgctccg ccggcgagcg agatgtcctt gccgcccgcg
      301 gcccgcttcg cctggtcgag ggcgacttcg atgccctgcg tgacgaaatg gaaggccgtg
      361 ccgccttgca tcctgagggg ctggcgcgcg tgatgcgtga gcacgaacac cgggtgatgg
      421 aacggcgggt tgtcgcccca ccagccgttc cacggctgct tcgcgtccca gcctccggga
      481 tggccgccga acatgttgcg ccccatgatc gtcgcgccga tgttggcgag ggactcctgc
      541 accacggcgc tgctctcgtt cacttcgccg ccttccatgc cgtgcatctc gcgccaggcc
      601 ttcagcggaa acacccactc gtgcagccgc atgccgccga cgccgagcgg gttgtccacg
      661 ctctgctgcg gcccggcggc gaagccgtcg agcgacatcg agatcctcaa gcgcagcttc
      721 atgctagccc tcgttgtaga tcgtatagcg gacgaagtcg ctgtgcggag tgaatttgtc
      781 atagaggccg cgcgcgcggt agttgttcag ccgcgtgtgc cagtagagcc gcggccagcc
      841 ctccttcttc atctcgtccc tcagccacgc gatcagctgc tcgccgatgc cgccggcgcg
      901 ccgcgcgggg tcgacgaaca ggtcgccgag ataacacacc ggcttcaggc ccagcgtgtg
      961 ctcgtggacc acatagttcg cgatgccgat gacaccgtgc ttctcatggt cggcgacgat
     1021 cgcatgcacc ggcgatgccg ggtccaggat gcgtgcccac gtgtggcggg tgagcgcttc
     1081 ggaaggcgtg cgcttgtaga agatgcagta gccgtcgaac agctcgcgcc agcgcttttc
     1141 gtcccgggcg gcggcgctgc gaacggttac ttccactaga ggggcttttt caggaagtga
     1201 gcgatctcgc cgacgatgcc gcggcggaag gtgagcacgc agacgacgaa gatgacgccc
     1261 tgggcgatgg tgacccaggc gccgaactgg gcgaggtagt tctccatgct gatcaccacc
     1321 agcgcgccgg cgaccgggcc gaagacggtg cccatgccac cgagcagcgt gatcagcacc
     1381 acttcgccgg acatcgacca gtgcgcgtcg gtgagcgagg cgagctggaa gacgatcgcc
     1441 ttggtggcgc cggcgagccc ggcgagggtg gcggagagga ggaaggcgag gaacttgtag
     1501 cgcgtggtgt cgtagccgag cgagatggcg cgcggctcgt tctcgcggat cgccttgagc
     1561 acctggccga agggcgaatg gatggtgcgg aagacgacca ggaagccggc ggcaaagatc
     1621 accagcacgg tgccgtacat cgcccagatg ctggtcaggt cgatgaagcc gaacaggcgg
     1681 ccgcgcggca ccgactggat gccgtcctcg ccgtgggtga acggcgcctg cagatagaag
     1741 aagtagatca tctgcgcgag cgccagggtg atcatcgcga aatagatgcc ctggcggcgg
     1801 atggcgagca gtcccgtcac gaacccgaga agtgcggcgc agaacgtccc gaagaggatc
     1861 gccgcctcgg gagggaagcc ccacatcctg gccgagtgcg cggcggcgta gcccgccgtg
     1921 ccgaggaaca tggcgtggcc gaaggacagc agcccgccga agccgaccag caggttgaag
     1981 gcgcaggcga agag
//
LOCUS       DASYJA010000094         2134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1468473, whole genome shotgun sequence.
ACCESSION   DASYJA010000094 DASYJA010000000
VERSION     DASYJA010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468473"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VGO02_02030"
     CDS             complement(<1..419)
                     /locus_tag="VGO02_02030"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEV7476496.1"
                     /translation="MRCLAFFLQVFFVLTATAQDWPKQKAIHIVVGFAPASTTDLVAR
                     LVAPKLSEALGQSVIIENKPGAGGNLAAQQAKRAAPDGYTLFATSVAYAVNPSLYADA
                     GYDPITDFIPVILAPSTPNIITVNPAVPAKNLQELIEL"
     gene            446..1354
                     /locus_tag="VGO02_02035"
     CDS             446..1354
                     /locus_tag="VGO02_02035"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HEV7476497.1"
                     /translation="MNILFSSEMEKLQSWLPLLSRALPDDRITTSLDEKIDVALVATP
                     PAGVLKKLSGARLIQSLWMGVEGLVGDPDYPKGVPLARLVDPGMVAAMSETVLAHVLD
                     WHRRHNQYREQQRARQWLRHTQYMASDRTVGLLGLGELGGDVARKLRALGFNVCGWSR
                     RARQIDGVRGFTDLSAMLPECDALVCLLPLTARTRGILNSENLGKMKRKGILINVARG
                     AHIVKQDLVAALDSGQLAHAYLDVFDPEPLPKDDPLWAHPKVSITPHTAALTEPRTAV
                     PKIVENVERARRGEAPLNRVDFEAGY"
     gene            complement(1322..1675)
                     /locus_tag="VGO02_02040"
     CDS             complement(1322..1675)
                     /locus_tag="VGO02_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476498.1"
                     /translation="MSVVDDLDLARQQLESLKELFARHSGEADVRALRWLVALCRSAG
                     IDDEYCRSKLATVEELGAEMFTHGGHRRFSTDLLRQRILDALELFESRLYSLALLRSP
                     RQPASSIARFEVHPV"
     gene            1674..2123
                     /locus_tag="VGO02_02045"
     CDS             1674..2123
                     /locus_tag="VGO02_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476499.1"
                     /translation="MEPIVRAARRSDSRCGTILAYAARDSVSLRIDAWYNARMVRRAR
                     RIAAWGALLAMALHALSPLAAPAPRAGAPGLHDICTSAPAAPQPAGDPSSSHCALCVA
                     SASIASPSVVLGVFVAEFENHFFFNERPSYTRPPHPAARPRGPPAGA"
BASE COUNT          353 a          730 c          716 g          335 t
ORIGIN      
        1 agctcgatca gctcctgcag gttcttcgcc ggcaccgcgg gattcaccgt gatgatgttg
       61 ggcgtgctgg gcgcgaggat caccgggatg aaatcggtga tcgggtcgta gccggcatcc
      121 gcgtacaggc tcgggtttac cgcgtaggcg acgctggtgg cgaacagcgt gtagccgtcg
      181 ggcgcggcgc gctttgcctg ctgcgcggcc aggttgccgc cggcgccggg cttgttctcg
      241 atgatcaccg actggccgag cgcctcgctc agcttgggcg ccaccagccg cgcgaccaga
      301 tcggtggtgc ttgccggcgc gaagccgacc acgatgtgga tggctttctg cttcggccag
      361 tcctgggcgg ttgccgtcaa aacaaaaaaa acctgcagga aaaaggcgag gcacctcatc
      421 gctcaaatgc tagcatcctg ccgccgtgaa catcttgttt tccagcgaaa tggagaagct
      481 gcagtcgtgg ctgccgctgc tcagccgcgc gctgccggat gaccgcatca ccacttcatt
      541 ggacgagaag atcgacgtcg cgctggtggc gacgccgccg gccggcgtgc tgaagaagct
      601 cagcggcgca agactgatcc agtcgctatg gatgggcgtc gaggggctgg tcggcgaccc
      661 cgactatccg aagggcgtgc cgctcgcgcg cctggtcgat cccggaatgg ttgccgcgat
      721 gtccgagaca gtgctcgcgc atgttctcga ctggcaccgc cgccacaacc agtaccgcga
      781 gcagcagcgc gcccggcagt ggctgcggca tacgcaatac atggcctcgg accgcaccgt
      841 cggcctgctc ggcctgggcg agctcggcgg cgacgtcgcg cgcaagctgc gcgcgctggg
      901 cttcaacgtg tgcggctgga gccgccgcgc aagacagatc gacggggtga ggggcttcac
      961 cgacctgtcg gcgatgctcc ccgaatgcga cgcgctcgta tgcctgcttc ctcttaccgc
     1021 ccggacaaga gggatcctga attcagagaa cctcggaaaa atgaaacgca aaggcatcct
     1081 gatcaacgtc gcgcgcggcg cgcacattgt gaagcaggac cttgtcgccg ccctcgactc
     1141 ggggcagctg gcgcacgcct acctcgacgt gttcgacccc gagccgctgc caaaagacga
     1201 tccgctgtgg gcccacccga aagtcagcat cacgccgcac accgccgcgc tgaccgagcc
     1261 gcgcaccgcg gtgccgaaga tcgtggagaa cgtcgagcgc gcccgccggg gcgaggcgcc
     1321 gctaaaccgg gtggacttcg aagcgggcta ttgacgacgc cggctggcgc gggctgcgca
     1381 gcaaggcgag ggagtagagg cggctctcga aaagctcgag cgcgtccaga atgcgttgac
     1441 gcagcaaatc ggtgctgaaa cggcggtggc cgccgtgcgt gaacatctcg gcgcccagct
     1501 cctccaccgt cgcaagcttg ctgcggcagt actcgtcgtc gatccccgcg ctccggcaca
     1561 aggccaccag ccagcgcagc gcgcggacat cggcctcgcc ggaatggcgc gcgaaaagct
     1621 ccttcaggga ctcgagctgc tgtcgggcga gatcgagatc gtcgacaacg gacatggagc
     1681 cgattgtccg cgctgcgcgc cggagcgata gccgttgcgg cacgatcctg gcgtacgccg
     1741 cgcgcgacag cgtgtcattg cgaatcgacg cgtggtataa cgcgcgcatg gttcgccgcg
     1801 cgcgacgcat tgcagcatgg ggggcgctcc tcgcgatggc gctgcacgcg ctctcgcccc
     1861 tggccgcccc tgcgccgcgc gccggggccc ccgggctgca cgacatctgc acctccgccc
     1921 cggccgcgcc gcagcccgcc ggcgacccga gcagcagcca ttgcgccctg tgcgtggcgt
     1981 cggcttcgat cgcgagcccg agcgtcgttc tgggcgtctt cgtcgcagaa ttcgagaatc
     2041 atttcttctt caatgagcgg ccgtcctata cccggccgcc tcaccccgca gcccgtcccc
     2101 gcggaccccc tgccggcgcc tgagcgccgg actg
//
LOCUS       DASYJA010000095         8210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1014861, whole genome shotgun sequence.
ACCESSION   DASYJA010000095 DASYJA010000000
VERSION     DASYJA010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8210
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014861"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VGO02_02050"
     CDS             <1..379
                     /locus_tag="VGO02_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV7476500.1"
                     /translation="EWYDDSKGTNVGATVAALRGLGKRVVLIAGGEGKGQDFTPLAEP
                     AREIVSCALLIGRDAPLIEKALGGVRVERCASLEEAVKRAAAIAKAGEAVLLSPACAS
                     FDMFRDYRQRGEVFAAAVKGLPS"
     gene            376..1539
                     /gene="ftsW"
                     /locus_tag="VGO02_02055"
     CDS             376..1539
                     /gene="ftsW"
                     /locus_tag="VGO02_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEV7476501.1"
                     /translation="MSATFVFAKSAQSTEYDRPLAWAALLLAATGLVMVYSASIATAE
                     SARYTGHNAAFYLARHGLVLGAALTAAIMVFLVPAKWWQHAAPWLFMFGVALLVLVLI
                     PGFGREVNGARRWLDLPAFSLQPSELVKLAAVLYAADYTARKHAVLKSFRRGLLPMLA
                     VMLLVSWLLLREPDFGALVVIAVTTFGILFLGGMNGRHFAALVGMLAAGFALLVIVSP
                     YRMQRIFGFMDPWADPLGRGYQLSHALIAFGRGEWLGVGLGASVEKLHYLPEAHTDFL
                     LAVIAEELGFAGVLLVIALFVWIVVRAFAIGRQASLRDRHFAALTALGIGIWIGFQAL
                     INMGVNMGLLPTKGLTLPLMSYGGSGLLVNFVALAILLRIDWENRQLARGLHA"
     gene            1536..2603
                     /gene="murG"
                     /locus_tag="VGO02_02060"
     CDS             1536..2603
                     /gene="murG"
                     /locus_tag="VGO02_02060"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEV7476502.1"
                     /translation="MSRVALIMAGGTGGHIFPGLAVAAQLRVAGWDVVWMGARGGMEE
                     RLVPPHGYRTAWIRARAARGKGIVQKLLLPANLLLSFWESLRHLRRIRPDVVLGLGGY
                     VAFPGGMMASLLGRPLALHEQNAIAGLANRVLAGVSDKVMVAFPGALKDAEWTGNPVR
                     AEIAAIEPPQARFGARSGALRILVVGGSLGAQALNEALPRAISLLKDRPIVVHQSGEK
                     HLDALRSNYSAAGAAGELVAFIDDMARRYAEADLVICRAGAVTVAELSAGGMASILVP
                     FPHAVDDHQTANARFLADQGAAILIQQRDLTPEGLAALIGSLDRSKLLEMARKARALG
                     KPDAAQLVARRCMQLAEAGVA"
     gene            2600..3973
                     /gene="murC"
                     /locus_tag="VGO02_02065"
     CDS             2600..3973
                     /gene="murC"
                     /locus_tag="VGO02_02065"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEV7476503.1"
                     /translation="MKHKVRRIHFVGIGGSGMSGIAEVLVNLGYQVSGSDLAENAATR
                     RLAKLGAKLSLGHKPEHIEGADAVVVSTAVRADNPEVMAARAKRVPLVPRALMLAELM
                     RLKQGIAIAGTHGKTTTTSLVASVLAEGGLDPTFVIGGRLTAAGSNARLGAGDFIVVE
                     ADESDASFLHLQPVIAVVTNIDADHMDTYQHDFARLRQAFVQFLQNLPFYGAAVLCAD
                     DVHVREILPFVSKPVLTYGTAADATVRAENITHDAGRMRFRARLGKTAFDVTLNLPGH
                     HNVLNALAAIAVATELGVAEKSIVKALAEFHGVGRRFQRYGDMPLEGGGAFTLIDDYG
                     HHPAEMAATLEAARGAFPGRRLVLAFQPHRYTRTRDLFEDFVRVLSTVDALVLAEVYA
                     AGEPPIVAADGRSLARAVRVAGQVEPVFVEDIAEMPAAIRRIAADGDVVVTMGAGSIG
                     NVPAQMT"
     gene            3976..4860
                     /gene="murB"
                     /locus_tag="VGO02_02070"
     CDS             3976..4860
                     /gene="murB"
                     /locus_tag="VGO02_02070"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV7476504.1"
                     /translation="MAEPLQFTALRDEPMARHVSWRAGGKADRFYAPLHLADLSNFLK
                     QVAPNEPLLFVGLGSNLLVRDGGFRGTVILMHSAELHAEMRDGRVYAQAGVAAPKVAR
                     FAAMQQLAGAEFLAGIPGTVGGALAMNAGCYGSETWDVVERVQTIDRNGTIFLRSRNE
                     FEIGYRHCHFPKEEWFIAAFFKLPSGDGEASRVRIKELLAKRIATQPLSLPNAGSVFR
                     NPPGDHAARLIESCGLKGLARGGASVSEKHANFIVNPKGVAKAADIEWLILEVKRRVR
                     LENGVDLVPEVRIVGEAQ"
     gene            4857..5768
                     /locus_tag="VGO02_02075"
     CDS             4857..5768
                     /locus_tag="VGO02_02075"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEV7476505.1"
                     /translation="MNFGKVAVLMGGTSAEREISLMSGNGVLAALRSKGVDAHSFDPK
                     ERDLFDLKKEGFARCFIALHGRGGEDGTIQGALEMLGIPYTGSGVMGSAIGMDKWRTK
                     MVWIANKLPTPRYRILTGKEDWRAVARELKLPLIVKPANEGSTLGLTKVTSVKQLPEA
                     YELAAKKYRDIALAEQFIDGPEYTASVLGEEALPLIRIEAPKGNYDYQNKYFTDDTKY
                     HCPSGLPAKKEKDLQALTLRAFRTVGCRGWGRVDIMLDKRSKPWLLEVNTSPGMTGHS
                     LVPMAAKAVGVPYEDLCVRILEMARVG"
     gene            5758..6468
                     /locus_tag="VGO02_02080"
     CDS             5758..6468
                     /locus_tag="VGO02_02080"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEV7476506.1"
                     /translation="MWDNPRLLNLAAGILVGVAALIFGVVLLTMLLRSPLAPLREVRV
                     ASALEHTSKQDIEQALRGRIAGNFFAVQPAEIRAGLQQLPWVRRVTVRRVWPDSIEVG
                     IEEHVAFARWGNDALVNTHGERFAAKTDQVLPLFLAPAGHEAEVTLRYGSFTALLAPL
                     GVVPERVVLSQRLAWQLRLSNGLHVMLGRDGDQAEARLRRFVGAYEATLKGMQRKHEY
                     VDLRYPNGFALRIPELKG"
     gene            6473..7702
                     /gene="ftsA"
                     /locus_tag="VGO02_02085"
     CDS             6473..7702
                     /gene="ftsA"
                     /locus_tag="VGO02_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEV7476507.1"
                     /translation="MPKENKNLIVALDIGTSKVACLVAELRPDGTLAILGMGGHESKG
                     LKKGVVVNIEATVGAVQRALEEAELMADTKITSAFVGIAGSHIRSFNSTGMVAVKDRE
                     VNALDVERAVDTARAVNIPTDQQILHVLRQEFIIDGQEDVREPIGMSGVRLEVKVHIV
                     TGAVSAAQNIIKCVRRCGLEVHDLILQPLASARAVLSEDEKVLGVCLLDIGGGTTDIA
                     IFTHGAIRHTAVIPIAGDQITNDIAMALRTPTADAETIKTRHGVALRQLADANDMIEV
                     PGIGERAPRSMSRQTLAEVIEPRVEELFTLVQQTLRESGFEELLSSGVVLTGGSSVMQ
                     GMVELGEEIFHMPVRIGLPKYQGGLAEVVRTPRYATAVGLLLDGVSQVQHGQLSRQGG
                     SVRAVLSRMREWFQRNF"
     gene            7728..>8210
                     /locus_tag="VGO02_02090"
     CDS             7728..>8210
                     /locus_tag="VGO02_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV7476508.1"
                     /translation="MFEIVDSQATGPIIRVIGVGGAGGNAVNHMIEQGVKGVEFIAVN
                     TDAQVLARNKARNQIQLGSSGLGAGAKPEEARAVAIADRERIEEAVAGSHMVFITAGM
                     GGGTGTGAAPVIAEIARGMGILTVAVVTKPFTFEGSKRMKIAEQGLEELGPHVDSLIV
                     I"
BASE COUNT         1300 a         2746 c         2874 g         1290 t
ORIGIN      
        1 cgagtggtac gacgactcga aggggacgaa cgtcggcgcg accgtcgccg cgctgcgcgg
       61 cttgggcaag cgggtcgtgc tgatcgccgg cggcgagggc aaggggcagg acttcacgcc
      121 gctcgccgag ccggcgcgcg agatcgtctc gtgcgcgctg ctgatcgggc gcgacgcgcc
      181 gctgatcgag aaagcgctcg gcggcgtgcg cgtcgagcgc tgcgcctcgc ttgaggaggc
      241 ggtgaagcgg gccgctgcga tcgccaaggc gggcgaagca gtattgctgt cgccggcctg
      301 cgccagcttc gacatgtttc gcgactaccg ccaacgcggc gaggtatttg ccgctgcggt
      361 gaaggggctg ccgtcttgag cgcgaccttc gtcttcgcga aaagcgcgca gagcaccgaa
      421 tacgaccggc cgctcgcctg ggcggcgctg ctgctggccg cgaccggcct ggtcatggtg
      481 tactcggcct ccatcgccac cgccgagtcg gcgcgctata ccggccacaa cgcggccttt
      541 tacctggcgc ggcacggact ggtgctcggc gccgcgctca ccgcggcgat catggtgttc
      601 ctggtgccgg cgaaatggtg gcagcacgca gcgccgtggc ttttcatgtt cggcgtcgcg
      661 ctgctggtgc tggtcctgat cccgggattc ggccgcgagg tgaacggggc gcggcgctgg
      721 ctcgacctgc cggcattcag cctgcagccc tccgagctgg ttaagctcgc tgccgtgctg
      781 tatgcggccg actacaccgc gcgcaagcac gcggtgctga agagcttccg caggggcctg
      841 ctgccgatgc tcgcggtcat gctgctggtg tcgtggctgc tgctgcgcga gcccgacttc
      901 ggcgcgctgg tggtaatcgc cgtcaccacc ttcggcatcc tgtttctcgg cggcatgaat
      961 ggcaggcact tcgccgcgct ggtcggcatg ctggcggcgg gcttcgccct gctcgtcata
     1021 gtgtcgccgt accgcatgca gcgcatcttc ggcttcatgg atccctgggc cgatccgctc
     1081 ggcaggggct accagctgtc gcacgcgctg atcgccttcg ggcgcggcga gtggctggga
     1141 gtgggcctcg gcgcgagcgt cgagaagctg cactacctgc ccgaagcgca caccgacttc
     1201 ctgctggcgg tgatcgccga agagctcggc ttcgccggcg tactgctggt gatcgcgctc
     1261 tttgtctgga tcgtggtgcg cgcctttgcc atcggccgcc aggcgagcct gcgcgatcgc
     1321 cacttcgccg cgctgacggc tctgggcatc ggcatctgga tcggcttcca ggcgctcatc
     1381 aacatgggcg tcaacatggg cctgctgccc accaagggcc tgacgctgcc gctcatgagc
     1441 tacggcgggt cggggctgct ggtgaacttc gtcgcgctgg ccatcctcct gcgcatcgac
     1501 tgggagaacc gccagctcgc cagaggattg catgcatgag ccgcgtcgcg ctcatcatgg
     1561 cgggcggcac cggcgggcac atttttcccg ggctggccgt cgccgcgcag ctgcgcgtcg
     1621 ccggctggga cgtggtgtgg atgggagccc gcggcgggat ggaggagcgc ctggtgccgc
     1681 cgcacggcta ccgcactgcg tggatccgcg cgcgcgcggc gcgcggcaag ggcatcgtgc
     1741 agaagctgct gctgccggca aacctgctgc tttcgttctg ggagagcctg cggcacctgc
     1801 gccgcatccg gcccgacgtc gtgctcggcc tgggcggcta cgtcgccttc ccgggcggca
     1861 tgatggcctc gctgctcggc cggccgctcg cgctgcacga gcaaaacgcg atcgccggtc
     1921 tcgccaaccg cgtgctggcc ggcgtttcgg acaaagtgat ggtggctttc cccggcgcgc
     1981 tgaaggacgc cgagtggacc ggcaacccgg tgcgcgcgga aattgcggcg atcgagccgc
     2041 cgcaggcccg cttcggcgca cgcagcggcg cgctgcgaat tctggtggta ggcggcagcc
     2101 tcggcgcgca ggcgttgaac gaggcgcttc cccgggcgat cagcctgctg aaagaccgtc
     2161 cgatcgtggt gcaccagtcg ggcgagaagc acctcgacgc cctgagaagc aactactcag
     2221 ccgccggggc ggccggagag ctcgtcgcgt tcatcgacga catggcgcgg cgctatgccg
     2281 aagccgacct tgtcatctgc cgggcgggag ccgttaccgt ggcggaactc tcggcgggtg
     2341 gcatggcgag catcctggtg ccgttcccgc acgcggtgga cgaccaccag acggcgaacg
     2401 cgcgcttcct ggccgaccag ggcgcggcga tcctcatcca gcagcgcgac ctcacgccgg
     2461 aggggctggc ggcgctcatc ggctcgctcg accgctcgaa gctgctcgag atggcgcgca
     2521 aagcgcgcgc gctcggcaag cccgatgcgg cgcagctcgt ggcgcggcgc tgcatgcagc
     2581 ttgccgaagc gggggttgca tgaagcacaa ggtgcggcgc atccatttcg tcggcatcgg
     2641 cggctccggc atgtcgggca tcgcagaagt gctggtgaac ctcggctacc aggtgagcgg
     2701 ctcggacctc gcagagaacg ccgcgacgcg ccgcctggcg aagctcggcg caaagctgtc
     2761 tctcggccac aaacccgagc acatcgaggg tgccgacgca gtggtggtct cgactgccgt
     2821 gcgcgccgac aaccccgagg tgatggcggc gcgcgccaag cgcgtcccgc tggtgccgcg
     2881 cgcgctgatg ctcgccgagc tgatgcggct caagcagggc atcgccatcg ccggcaccca
     2941 cggcaaaacg accaccacca gcctggtcgc gagcgtgctc gccgaaggcg gcctcgatcc
     3001 gactttcgtc atcggcgggc gcctcaccgc ggcgggctcc aatgcgcgct tgggcgccgg
     3061 cgacttcatc gtggtcgagg ctgacgagtc ggacgcctcg ttcctgcacc tgcagccggt
     3121 gatcgcagtg gtaaccaaca tcgacgccga tcacatggac acctaccagc acgacttcgc
     3181 ccgcctgcgg caggcgttcg tccagttcct gcagaacctg cctttctacg gcgccgcggt
     3241 gctgtgcgcc gacgacgtgc acgtgcgcga aatcctgccc ttcgtctcca agccggtcct
     3301 cacctacggc accgcggcgg atgcaacggt gcgcgccgag aacatcaccc acgacgccgg
     3361 gcgcatgcgc ttccgcgcgc ggctcggcaa gacggctttc gacgtcacgc tcaatctgcc
     3421 gggtcaccac aacgtgctca acgcgctcgc cgccatcgcc gtcgccaccg agctcggcgt
     3481 ggcggagaaa tccatcgtca aggcgctggc ggagttccat ggcgtcggcc gccgcttcca
     3541 gcgctacggc gacatgcctc tcgaaggcgg cggcgcattc accctcatcg acgactacgg
     3601 ccaccacccg gcggagatgg cggccacgct cgaggcggcg cgcggcgcct ttcccggccg
     3661 gcgcctggtg ctcgcgttcc agccgcatcg ctatacgcgc acgcgcgacc tgttcgagga
     3721 tttcgtcagg gtcctgtcca ccgtcgacgc gctggtcctc gccgaggtct atgccgcggg
     3781 cgagccgccg atcgtcgccg ccgacgggcg ctcgctcgct cgcgcagtgc gcgtcgcggg
     3841 ccaggtcgag ccggtgttcg tcgaggacat cgccgagatg ccggcggcga tccgccgcat
     3901 cgccgcagac ggcgacgtgg tcgtcaccat gggcgcgggc tcgatcggca acgtccctgc
     3961 gcaaatgaca tgaacatggc cgagcccctc caattcactg cactgcgcga cgagccgatg
     4021 gcgcggcacg tcagctggcg cgccggcggg aaggcggacc ggttttacgc acctttgcac
     4081 cttgcggatt tgagcaattt tctcaagcaa gttgcgccga acgaaccgct gctgttcgtc
     4141 ggcctgggct cgaacctgct ggtgcgcgac ggcggctttc gcggcacggt gatcctcatg
     4201 cactcggctg agctgcatgc cgaaatgcgc gacgggcgcg tctacgcgca agccggcgtc
     4261 gccgcgccga aggtcgcgcg cttcgccgct atgcagcagc tcgccggcgc ggagtttctc
     4321 gccgggattc cgggaaccgt cggcggcgcg ctcgccatga atgccggctg ctatggcagc
     4381 gagacctggg acgtcgtgga acgcgtgcag acgatcgacc gcaatggaac cattttcctg
     4441 agaagcagga acgagttcga aatcggctac cggcattgcc atttcccgaa agaggaatgg
     4501 ttcatcgctg cattcttcaa gctgccttcc ggcgacggcg aggcatcccg cgtgcgcatc
     4561 aaggagctgc tcgccaagcg catcgcgacg cagccgctgt cgctgcccaa cgcgggcagc
     4621 gtgttccgca atccgccggg cgaccacgcg gcgcgcctga tcgagtcatg cggcctgaaa
     4681 ggcctggcgc gcggcggcgc cagcgtttcc gagaagcacg ccaacttcat cgtcaatccg
     4741 aagggcgtgg cgaaggccgc ggacatcgag tggctgattc tcgaggtcaa gcgcagggtt
     4801 cggcttgaga acggtgtgga ccttgtcccc gaagtgcgca tcgtcgggga ggcgcaatga
     4861 acttcggcaa ggttgccgtg ctgatgggcg gcacttccgc cgagcgagag atctcgctga
     4921 tgagcggcaa cggggtgctc gcagcgctgc gctcgaaggg cgtggacgcg cattcgttcg
     4981 acccgaagga gcgcgacctt ttcgacctga agaaggaagg cttcgcgcgc tgcttcatcg
     5041 cgctgcacgg ccgcggcggc gaggacggca ccatccaggg ggcgctcgag atgctcggca
     5101 tcccttacac gggcagcggg gtgatgggct cggcgatcgg catggacaag tggcgcacca
     5161 agatggtctg gatcgccaac aagctgccga cgccgcgcta ccggattctg acgggcaaag
     5221 aggattggag ggcggtcgcg cgcgagctca agctgccgct catcgtcaag ccggcgaacg
     5281 agggctcgac gctcggcctg accaaggtca cctccgtaaa gcagctaccg gaggcctacg
     5341 agctggcggc gaagaaatac cgcgacatcg cgctcgccga gcagttcatc gacggtcccg
     5401 agtacaccgc gagcgtgctt ggcgaggagg cgctgccgct gatccgcatc gaggcgccaa
     5461 agggcaacta cgactaccag aacaaatatt tcaccgacga caccaagtac cactgcccga
     5521 gcgggctgcc ggcgaagaag gaaaaggatc tgcaggcgct gacgctgcgt gcatttcgca
     5581 cggtcggatg ccgtggctgg ggccgcgtcg acatcatgct ggacaagcga agcaagccct
     5641 ggctcctcga ggtgaatacc tcgcctggca tgaccggcca cagtctggtg ccgatggcgg
     5701 cgaaagccgt cggcgtcccc tacgaggatc tgtgcgtgcg catcctggag atggcccgtg
     5761 tgggataacc cgcgcctgct gaatctcgcc gccgggatcc tggtcggcgt cgcggctctc
     5821 atcttcggcg tcgtgctgct gacgatgctg ctgcgctcgc cgctcgcgcc gctgcgcgag
     5881 gtgcgcgtcg ccagcgcgct cgagcacacc tcgaagcagg acatcgagca ggcgctgcgc
     5941 ggccgtatcg ccggcaactt cttcgccgtc cagccggccg agatccgcgc cggcctgcag
     6001 cagctgccct gggtgcgccg cgtgacggtg cgccgcgtct ggcccgacag catcgaggtc
     6061 ggcatcgaag agcacgtcgc gttcgcgcgc tggggcaatg atgcgctggt caatacgcat
     6121 ggcgagcggt tcgccgcgaa gaccgaccag gtcctgccgc tgttcctcgc gccggcgggc
     6181 cacgaagcgg aagtgacgct gcgctacggc agcttcacgg cgctgctggc gccgctcggc
     6241 gtggtgcccg agcgcgtggt gctcagccag cgcctcgcct ggcagctgcg cctgagcaac
     6301 gggctgcacg tcatgctcgg acgcgacggc gaccaagcgg aggcgcgcct gcgccgcttc
     6361 gtcggcgcct acgaagccac cttgaagggc atgcagcgca agcacgagta cgtcgacctg
     6421 cgctatccca acggctttgc gctgcgcata ccggagctaa aaggctgagc ggatgccaaa
     6481 agagaacaag aacctgatcg tcgctctgga catcggcacc tcgaaggtcg cctgcctagt
     6541 ggccgagctg cgccccgacg gcacgctcgc catcctcggc atgggcgggc acgagtcgaa
     6601 gggcctgaag aagggcgtgg tggtgaacat cgaggccacg gtcggcgcgg tgcagcgcgc
     6661 gcttgaggaa gccgagctga tggccgacac caagatcacc tcggccttcg tcggcatcgc
     6721 cggcagccac atccgcagct tcaactcgac cggcatggtg gcggtcaagg accgcgaggt
     6781 gaacgcgctc gacgtcgagc gcgccgtcga cacggcgcgc gcggtgaaca tccccaccga
     6841 ccagcagatc ctgcacgtgc tgcgccagga attcatcatc gacggccagg aggacgtgcg
     6901 cgagccgatc ggcatgtcgg gcgtgcgcct ggaggtgaag gtgcacatcg tcaccggtgc
     6961 ggtgtcggcg gcgcagaaca tcatcaagtg cgtgcgccgc tgcggcctcg aggtgcacga
     7021 tttgatcctg cagccgctcg cctcggcgcg cgccgtgctc tccgaggacg agaaggtcct
     7081 cggcgtgtgc ctgctcgaca tcggcggcgg caccaccgac atcgccatct tcacgcacgg
     7141 cgccatccgc cacacggcgg tgatcccgat cgcaggcgac cagatcacca acgacatcgc
     7201 gatggccctg cgcacgccca ccgccgacgc cgagacgatc aagacgcgcc acggcgtcgc
     7261 gctgcggcag ctggccgacg cgaacgacat gatcgaggtg cccggcatcg gcgagcgcgc
     7321 gccgcgctcg atgtcgcgcc agacgctggc ggaggtaatc gagccgcgcg tcgaggagct
     7381 cttcacgctg gtgcagcaga cgctgcgcga gtcgggcttc gaggagctcc tgtcgagcgg
     7441 cgtggtgctg acgggagggt catcggtgat gcagggcatg gtcgagctcg gcgaggagat
     7501 cttccacatg ccggtgcgta tcggcctgcc gaagtaccag ggcgggctgg cggaggtcgt
     7561 gcgcacgccg cgctacgcca cggcggtggg actgctgctc gacggcgtct cgcaggtgca
     7621 gcacggacag ctttcacggc agggcggctc ggtcagggcc gtcctgtcgc ggatgaggga
     7681 gtggtttcag cgcaatttct aattcttaac ggcaaggagg caaggacatg ttcgaaatag
     7741 tcgatagtca ggcaaccgga ccgatcataa gagtgatcgg agtgggcggc gcgggcggca
     7801 acgccgtcaa ccacatgatc gagcagggcg tcaagggcgt ggaattcatc gccgtcaaca
     7861 ccgacgcgca ggtgctcgcc cgcaacaagg cgcgcaacca gatccagctc ggatcttcag
     7921 gactgggcgc tggcgccaag cccgaggaag cgcgcgcggt ggcgatcgcc gaccgcgagc
     7981 gcatcgagga ggctgtggcg ggctcgcaca tggtgtttat caccgcaggc atgggcgggg
     8041 gcaccggtac cggtgccgcc cccgtgatcg ctgagatcgc gcgcggcatg ggcatcctga
     8101 cggtggcggt ggtcaccaag ccgttcacct tcgagggctc caagcgcatg aagatcgccg
     8161 agcagggcct cgaggagctg gggccgcacg tcgactcgct gatcgtgatc
//
LOCUS       DASYJA010000096         4919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1831663, whole genome shotgun sequence.
ACCESSION   DASYJA010000096 DASYJA010000000
VERSION     DASYJA010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1831663"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /gene="argJ"
                     /locus_tag="VGO02_02095"
     CDS             complement(<1..1096)
                     /gene="argJ"
                     /locus_tag="VGO02_02095"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEV7476509.1"
                     /translation="MAVNLSAPDAKSLHAVRGVELGVAMAGIKKPDRKDLLVLRLPPG
                     AAVAGVFTQNRFCAAPVILARKHLKNPVRALVVNTGNANAGTGEDGLRRALQVCEALA
                     SHLGCDASQVLPFSTGVIMEPLPAERITAALPKALLGTADWLSAAQAIMTTDTVPKAF
                     SRKIRLSGGEAVVTGIAKGAGMIHPGMATMLGFVATDAALSRKRLDVLVRKVAERSFN
                     RITVDGDTSTNDAFMLMATGRGPAAASRRDLLKLEAALTDVARQLAQAIVRDGEGATK
                     FVTVRVEAGRGEEECRRVAYAIAHSPLVKTAFFASDPNLGRILAAIGNSGVPLDTAKV
                     DLYIDDVLVAKGGERNPAYREEQGAAAMKKA"
     gene            complement(1101..3896)
                     /gene="secA"
                     /locus_tag="VGO02_02100"
     CDS             complement(1101..3896)
                     /gene="secA"
                     /locus_tag="VGO02_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEV7476510.1"
                     /translation="MITKILFFLRTVLQTLLSIPTRLFGSRNERLLKQYSQAVQDINA
                     LEPVLAKLSDAQLRGKTDELKKKYADGATLDDLLPEAFAVVREASKRALRMRHFDVQL
                     LGGMVLHNGKIAEMKTGEGKTLVATLPAYLNALTGKGVHIVTVNDYLASRDAAWMGKI
                     YSFLGLSVGVNLPYHELELEERNSTKRAAYAADITYDTNNEFGFDYLRDNMAMHVEER
                     FQRGLPYAIVDEVDSILIDEARTPLIISGQAEDRTELYHRMDQVPPLLTPQKEEKKQD
                     QPEPPGDFFVDEKNHQIVLSEAGHQKAEEILARVGLLPQGASLYEPAYINLMHHLYAA
                     LRAHHLFHRDQHYVVQDDEVIIVDEFTGRLMQGRRWSDGLHQAVEAKEKVAIQNENQT
                     LASITFQNYFRMYGKLAGMTGTADTEAYEFQQIYGLEVVVIPTNVPMIRDDRLDQVYR
                     TAKEKYQAMINDIQACQARGQPVLVGTTSIENSELISGLLTKDKLAHQVLNAKQHARE
                     AEIVAQAGRPKMITIATNMAGRGTDIVLGGNVEKQVDFTVADEKIAENDKQQRIDKLR
                     SEWQSLHDAVVKSGGLHIIGTERHESRRVDNQLRGRSGRQGDPGSSRFYMSLEDSLLR
                     IFAGERINRIMVMLKMPEGEAIEHRMVTRSIESAQRKVEARNFDIRKQLLEYDDVAND
                     QRKTIYSLRNEVLESHDVSPRVADLRSGVLADMFRVHIPEDSVEEQWDVAGLENALKA
                     ELNLELPLRKWLEDEPELDDATMLARIAERAAAAYAAKVPSGAEASFRQYERYVMLQI
                     LDGHWREHLAALDHLRQGIHLRGYAQKNPKQEYKREAFELFGAMVEAVKLEVVKTLTA
                     VEIRAKEDVPQADDKLHVQNVQLQHGGEAEGGEGDAPAEQKKAQPTVRHAQKVGRNDP
                     CPCGSGKKYKHCHGKLT"
     gene            complement(3970..4902)
                     /locus_tag="VGO02_02105"
     CDS             complement(3970..4902)
                     /locus_tag="VGO02_02105"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEV7476511.1"
                     /translation="MQIILISDRLAKARSVSISKRHLLALGLFTISLVCAGSIGLYWL
                     TLRYASEVPLPGLQRLLLSAQEAEAERSRSFVQQNLNAMAVKLGEMQAQLTRLDALGE
                     RLSSISGVRDIRFTEAPGLGGASPTLLPPQNLSLAEFSEKLALLSRQVESRNDMLGVL
                     DAQLFEQAVKKRLLPTMMPVAASYNASSFGRRIDPFTGQWAMHEGIDFLADNGSPIHA
                     AAAGVVIFAGFHPQYGYVIDIDHGNDLVTRYAHCSKLYVREGDVLQRGRRIAESGSTG
                     RSTGPHLHFEVRYRGTAQNPAKFLEATQAVARTK"
BASE COUNT          722 a         1600 c         1666 g          931 t
ORIGIN      
        1 cggctttctt catcgcggcc gcgccctgct cctcgcggta ggcgggattg cgctcaccgc
       61 ccttcgccac gaggacatcg tcgatgtaga ggtcgacctt cgccgtgtcg agcggcacgc
      121 ccgaattgcc gatcgcggcg aggatccttc ccaggttcgg atctgaggcg aagaacgcgg
      181 tcttgacgag cggcgaatgc gcgatggcat aggcgacgcg ccggcactcc tcctcgccgc
      241 gccccgcctc gacccgcacg gtgacgaact tcgtcgcgcc ttcgccgtcg cgcacgatcg
      301 cctgggcaag ctggcgcgcg acgtcggtga gcgcagcctc gagcttgagc aggtcgcggc
      361 gcgacgccgc ggcgggcccg cggccggtcg ccatcagcat gaaggcgtcg ttggtcgagg
      421 tgtcgccatc caccgtgatg cggttgaacg agcgctcggc gaccttgcgc accaggacat
      481 cgagcctctt gcgcgagagc gcggcgtcgg tggcgacgaa gccgagcatg gtggccatgc
      541 cggggtggat catgccggcg cccttggcga tgccggtcac caccgcttcc ccgccggaca
      601 gccggatttt tctcgaaaag gccttcggca ccgtatcggt ggtcatgatg gcctgcgcgg
      661 cgctcagcca gtcggccgtg ccgagcagtg ccttcggcag cgccgcggtg atgcgctcgg
      721 cgggcagcgg ctccatgatg acgccggtcg agaagggcag cacctgcgag gcgtcgcagc
      781 cgaggtggct ggcgagcgct tcgcaaacct gcagcgcgcg cctcagcccg tcctcgccgg
      841 taccggcgtt cgcgttccct gtgttgacca ccagggcgcg gaccggattc ttcagatgct
      901 ttctggcgag gataaccggc gcggcgcaga acctgttctg cgtgaatacg ccggcgaccg
      961 cggcgcccgg cggcaagcgc agcaccagca ggtctttccg atcgggcttc ttgatgccgg
     1021 ccatcgccac gccgagctct acgccgcgca cggcgtgcag cgacttcgcg tccggggcgg
     1081 agaggttgac cgccatgcgg ctaggtcagc ttgccgtggc agtgcttgta cttcttgccc
     1141 gagccgcacg ggcagggatc gttgcgcccg accttctgcg cgtggcgcac cgtcggctgg
     1201 gctttcttct gctcggcggg tgcgtcgcct tccccgcctt cggcctcgcc gccgtgctgc
     1261 agctgcacgt tctgcacgtg cagcttgtcg tcggcctgcg gcacatcctc cttggcgcgg
     1321 atctcgaccg cggtaagggt cttcacgact tcgagcttga ccgcctccac catggcgccg
     1381 aacagctcga acgcctcgcg cttgtattcc tgcttcgggt tcttctgtgc gtagcctcgc
     1441 agatggatgc cctggcgcag atggtcgagg gctgcgaggt gctcgcgcca gtggccgtcg
     1501 aggatctgca gcatgacgta gcgctcgtac tggcggaacg acgcctcggc gccggacggg
     1561 acttttgccg cgtaggcggc cgcggcacgc tcggcgatgc gcgcgagcat cgtcgcgtcg
     1621 tcgagctcgg gctcgtcttc cagccatttg cgcagcggca gctcgaggtt gagctcggcc
     1681 ttgagcgcgt tctccagccc tgccacgtcc cactgctcct cgacgctgtc ctcggggatg
     1741 tgcacgcgga acatgtcggc gagcacgccc gagcgcaggt cggccaccct gggcgagacg
     1801 tcgtgcgact ccagcacctc gttgcgcagc gagtagatgg tttttctctg gtcgttggcg
     1861 acgtcgtcgt actcgagcag ctgcttgcgg atgtcgaagt tgcgcgcctc gaccttcctc
     1921 tgcgcgctct cgatggagcg cgtcaccatg cggtgctcga tcgcctcgcc ctcgggcatc
     1981 ttgagcatca ccatgatgcg gttgatgcgc tcgccggcga agatcctgag cagcgaatcc
     2041 tcgagcgaca tgtagaagcg cgagctgccc gggtcgccct ggcggccgga gcggccgcgc
     2101 agctggttgt cgacgcgccg cgactcgtgg cgctcggtgc cgatgatatg caggccaccc
     2161 gacttcacca ccgcgtcgtg cagcgactgc cactccgagc gcagcttgtc gatgcgctgc
     2221 tgcttgtcgt tctcggcaat cttctcgtcc gcgacggtga agtcgacctg cttctcgacg
     2281 ttgccgccga gcacgatgtc ggtgccgcgg cccgccatgt tggtggcgat ggtgatcatt
     2341 ttcggccggc cggcctgcgc cacgatctcg gcctcgcgcg cgtgctgctt ggcgttgagc
     2401 acctggtgcg cgagcttgtc cttcgtcagc agccccgaaa tcagctccga attctcgatc
     2461 gaggtggtgc ccaccagcac cggctggccg cgtgcctggc aggcctggat atcattgatc
     2521 atcgcctggt acttctcctt ggcggtgcgg tacacctggt cgaggcggtc gtcgcggatc
     2581 atcggcacgt tggtcgggat cacgaccacc tcgagtccgt agatctgctg gaactcgtag
     2641 gcctcggtgt cggccgtgcc ggtcatgccg gcaagcttgc cgtacatgcg gaagtagttc
     2701 tggaaggtga tcgaggcgag cgtctggttc tcgttctgga tcgccacctt ctccttcgcc
     2761 tccactgctt gatgcaaacc atccgaccag cgccggcctt gcatcaagcg accggtgaat
     2821 tcatcgacga tgatcacctc gtcgtcctgc acgacgtagt gctggtcgcg gtggaacagg
     2881 tggtgtgcgc gcagcgccgc gtagagatgg tgcatcaggt tgatgtacgc cggctcgtac
     2941 aggctcgcgc cctgcggcag caggccgacg cgcgccagga tctcctcggc tttctggtgg
     3001 ccggcctcgg acagcacgat ctggtggttc ttctcgtcga cgaagaagtc gcccggcggc
     3061 tcgggctggt cctgcttctt ctcttccttc tggggggtca gcagcggcgg cacctggtcc
     3121 atgcggtggt agagctcggt gcggtcctcg gcctggccgg agatgatgag cggggtgcgc
     3181 gcctcgtcga tcaggatcga gtccacctcg tcgacgatgg cgtagggcag gccgcgctgg
     3241 aagcgctcct cgacgtgcat cgccatgttg tcgcgcagat agtcgaagcc gaattcgttg
     3301 ttggtgtcgt aggtgatgtc ggcggcatag gccgcccgct tggtggagtt gcgctcctcc
     3361 aactcgagtt cgtggtaggg caggttcacc ccgacgctca ggcccaggaa gctgtagatc
     3421 ttccccatcc acgcggcatc gcggctggcg aggtagtcgt tgaccgtaac gatgtgcacg
     3481 cccttgccgg tgagggcgtt caggtacgcg ggcagtgtgg caaccagcgt cttgccctcg
     3541 cctgtcttca tctcggcgat cttgccgttg tgcagcacca tgccgccaag cagctgcacg
     3601 tcgaagtgcc gcatgcgcag cgcgcgcttc gacgcttcac gcacgactgc aaacgcttcc
     3661 ggcagaagat cgtcgagcgt ggcgccgtcg gcgtacttct ttttcagctc atccgttttt
     3721 ccgcgcagct gcgcatcgga gagcttggcc agcaccggct cgagcgcgtt gatgtcttga
     3781 acggcttgcg agtactgctt gagcagccgc tcgttgcggc tgccgaacag ccgcgttgga
     3841 atgcttagaa gagtttgcag caccgttcgg aggaaaaaga gaatcttggt gatcaattgg
     3901 cgaagcccgc gcggacgctg tccacccgga cccctgcaaa acagctgatt ttagcggaaa
     3961 agcggagtac tacttggtgc gggctaccgc ttgggtcgcc tcgaggaact tggcggggtt
     4021 ctgcgccgtg ccgcggtagc gcacctcgaa gtgcaggtgc gggccggtgg agcggccggt
     4081 cgagcccgac tcggcgatcc tgcggccgcg ctgcaggacg tcgccctcgc gcacgtagag
     4141 cttcgagcag tgcgcatagc gcgtgaccag gtcgttgcca tggtcgatgt caatgacata
     4201 gccgtactgg ggatggaagc cggcgaagat cacgacgccg gccgcggcgg cgtggatcgg
     4261 cgagccattg tcggcgagga aatcgatgcc ttcgtgcatc gcccactgac cggtgaaggg
     4321 gtcgatgcgc cggccgaagc tcgatgcgtt gtaggacgcc gcgaccggca tcatcgtcgg
     4381 cagcaggcgc ttcttgaccg cctgctcgaa gagctgcgcg tcgagcacgc cgagcatgtc
     4441 gttgcggctt tccacctggc gcgaaagcag tgccagcttc tcgctgaact ctgcgagcga
     4501 caggttctgc ggcggcagca gcgtcggcga ggcgccgccc aggccggggg cctcggtaaa
     4561 gcggatgtcg cgcacgccgg agatcgagga caggcgctcg ccgagcgcgt cgaggcgcgt
     4621 cagctgcgcc tgcatctcac ccagcttgac cgccatcgcg ttgaggttct gctgcacgaa
     4681 ggagcgcgag cgctcggcct cggcctcctg cgccgagagg agaagcctct gcaggccggg
     4741 cagcggcact tcggaggcgt agcgcagggt gagccagtac agcccgatac tgccggcgca
     4801 gaccagggag atcgtgaaca gcccgagcgc cagcaggtgg cgcttcgaaa tgctcaccga
     4861 cctcgccttg gccaggcggt cggagatcag gataatctgc acttacaccc tcccaagac
//
LOCUS       DASYJA010000097         3543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2738818, whole genome shotgun sequence.
ACCESSION   DASYJA010000097 DASYJA010000000
VERSION     DASYJA010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2738818"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VGO02_02110"
     CDS             <1..356
                     /locus_tag="VGO02_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476512.1"
                     /translation="QGEGIIDIVNTYLGIALASGFVGLSLFCGFFLVVLVELLAAVRR
                     AGGRDGEPYALGQALFCALLGILVTICTTSSIGLIPVIYWSIAGLAQAYARVAAPARA
                     AETEPDGDPRLIVAS"
     gene            373..1728
                     /locus_tag="VGO02_02115"
     CDS             373..1728
                     /locus_tag="VGO02_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEV7476513.1"
                     /translation="MSHQAAGLRARVLRAGGWTLAGFALSQAIRFGANLVMTRLLLPE
                     MFGLMAIATMIMYGLALFSDLGLRQSIVQSGRGREAAFLNTAWAVQIARGFVIWAAAL
                     AVALGLAFANAIGSVPLGSVYAHPSLPPVVAMFSLCAAIAGFESTKLCEASRELSLGR
                     ITRIDLVSQSVGVLCMIAWAVLERSIWALVAGGLCATAARMLLSHGWLPGTANRWQWD
                     SAAFREIIGLGKWIFAASILGFFVNSADRLVLGALTSTAVLGVYVIAFLIFSSVEQLL
                     ARIVGEVAYPALSEVARERPAALRSAYYRFHLAVGLPAFFAAGLLVVCAEPLVALLYD
                     ARYAEAGWMLRILAVALLTLPFRVAMQCFVVLGEPAQMSVIGAIRLACLCAAVPLGWH
                     FFGLAGALWGIVLSYFSTLPTTLAFVVRHRLFDAARELALIPALLAGLAAGQAVSYVL
                     R"
     gene            complement(1709..2806)
                     /locus_tag="VGO02_02120"
     CDS             complement(1709..2806)
                     /locus_tag="VGO02_02120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV7476514.1"
                     /translation="MRITFVLPHAGMSGGNRVLAIYASRLQRRGHAVQVVSIPQHVPS
                     FARKLKSLLRGQGWPPDPEPEHSWFDGLEVPHRVLERVRAVSDADVPDADVVLATFWR
                     TGPWVAALSPSKGAKAILLQGYETSPGHEDPAIDAVWRLPLRKIVISRWLVELARERF
                     GDGDVHHVPNSVETAQFHASPRGRQARPTVGMLYGRFHAKGVDVAVSALQRVRERVPD
                     LKVLAFGAERVAPNVPLPPWVEFHYRPPQEALRHLYAQCDAWLCASRREGFHLPPLEA
                     MACRCPVVSTRVGGPMDLVEEGVNGFLVEVEDAAALAERLQQVLAFDAARWKRMSDAA
                     LATAQRYTWDDATALLEQALAEIAGATVVHS"
     gene            complement(2816..>3543)
                     /locus_tag="VGO02_02125"
     CDS             complement(2816..>3543)
                     /locus_tag="VGO02_02125"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEV7476515.1"
                     /translation="FPHVVFDDFFDPALLDKVLGEFPRPGAIKWQRFDNQQEIKLASA
                     VESNFGPVTRLLLYHLNSYTFLDFLSRVTGIENLISDPCFDGGGLHQIVRGGKLAVHA
                     DFNKHPRYGLDRRLNVLVYLNRDWREEYGGALELWDRNMTRSEAKIFPLFNRLVIFGT
                     TDFTYHGHPDPLQCPAELTRKSLALYYFTNGRPAEEISGEHSTLFRARANEELRPDLR
                     QRLRGAARELLPPILARQLRKYL"
BASE COUNT          492 a         1226 c         1221 g          604 t
ORIGIN      
        1 agcagggcga gggcatcatc gacatcgtca acacctatct cggcatcgcg ctggcgagcg
       61 gcttcgtcgg cctgtcgctc ttctgcggct ttttcctggt ggtgctggtc gagcttctcg
      121 ccgccgtgcg ccgcgccggg ggccgggacg gcgagcccta cgcgctcggc caggcgctct
      181 tctgcgccct gctcggcatc ctggtgacga tatgcaccac gagcagcatc ggtctgatcc
      241 cggtgatcta ctggtcgatc gccggcctgg cgcaggccta tgcgcgcgtt gcggcgccgg
      301 cgcgcgccgc tgagactgag ccagatggcg acccccgcct tatcgttgcg agctaggatt
      361 tcccaagccc ggatgtcgca ccaggcggcc ggcctgcgcg cgcgcgtgct gcgcgccggc
      421 ggctggaccc tcgccggctt cgcgctgagc caggcgatcc gcttcggcgc caacctggtg
      481 atgacgcggc tgctgctgcc cgagatgttc ggcctgatgg cgatcgccac gatgatcatg
      541 tacgggctcg cgctgttctc ggacctcggg ctgcgccaga gcatcgtgca gagcgggcgc
      601 ggccgcgaag cggccttcct gaataccgcc tgggcggtgc agatcgcgcg cggtttcgtg
      661 atctgggcag ccgcgctcgc cgtcgccctg ggtcttgcat tcgcgaacgc aatcggcagc
      721 gtgccgctcg gcagcgtgta cgcgcatccc agcctgccac cggtcgtggc catgttctcg
      781 ctttgcgccg cgatcgcggg cttcgagtcg accaagctgt gcgaagcgag ccgcgagctt
      841 tcgctcggca ggatcacccg gatcgacctc gtcagccaga gcgtgggcgt gctgtgcatg
      901 atcgcctggg cggtgctcga gcgctcgatc tgggcgctgg tcgcgggcgg cctgtgcgcc
      961 acggcggcaa gaatgctgct cagccacggc tggctgccgg ggaccgccaa ccgctggcag
     1021 tgggacagcg cggctttccg ggagatcatc ggcctcggca agtggatctt cgccgcctcg
     1081 atcctcggct ttttcgtcaa cagcgccgat cgcctggtcc tcggcgccct caccagcaca
     1141 gccgtgctcg gcgtctacgt catcgccttc ctgatcttca gctcggtcga gcagctgctc
     1201 gcccggatcg tcggcgaagt cgcgtatccc gcgctgagcg aagtcgcgcg cgagcggccg
     1261 gcggcgctgc gctcggcgta ctaccgcttt cacctcgcgg tcgggcttcc ggccttcttc
     1321 gccgcggggc tgctcgtcgt ttgcgccgag ccgctggtcg cgctgcttta cgatgcgcgc
     1381 tacgccgagg cgggctggat gctgcgcatc ctggcggtcg ctctgctcac gctgcccttc
     1441 cgcgtcgcca tgcagtgctt cgtggtgctc ggcgagcccg cgcagatgag cgtgatcggc
     1501 gccatccgtc tcgcctgcct ttgcgcggcg gtgccgctcg gctggcattt cttcggcctc
     1561 gccggcgcgc tctgggggat cgtgctcagc tacttctcca cgctgcccac cacgctcgcc
     1621 ttcgtcgtac ggcatcggct gttcgatgcg gcgagggaac tcgcgctcat tcccgccctc
     1681 ctagcgggcc tcgctgcagg ccaggcggtc agctatgtac tacggtagct cccgctatct
     1741 cggccagcgc ctgctcgagc agcgccgtcg cgtcgtccca ggtgtagcgc tgcgcggtgg
     1801 cgagcgcggc gtcggacatg cgcttccagc gcgcggcgtc aaaggccagg acctgctgca
     1861 gccgctcggc gagcgccgcc gcgtcctcca cttcgacgag aaagccgttg accccttcct
     1921 cgacgaggtc catcgggccg ccgacgcgcg tcgagaccac ggggcagcgg cacgccatcg
     1981 cctcgagcgg cggcaggtgg aacccctcgc gccggctcgc gcacagccat gcgtcgcact
     2041 gcgcatagag gtgccggagc gcctcctgcg gcgggcggta gtgaaactca acccatggcg
     2101 gcaacggcac gttgggcgca acgcgctcgg cgccgaacgc gagcaccttg agatcgggaa
     2161 ctcgctcgcg cacgcgctgc agggcgctga ccgcgacgtc cacccccttg gcgtgaaacc
     2221 ggccgtagag cattcccacc gtcgggcgtg cctggcggcc tctcggcgag gcgtggaact
     2281 gcgcggtctc gacgctgttc ggcacgtgat gcacgtcgcc gtcgccgaag cgctcgcgcg
     2341 ccagctcgac gagccacctg gagatcacga tcttgcgcaa tggcagccgc catacggcat
     2401 cgatcgccgg gtcttcatgg ccgggagaag tctcgtagcc ctgcagcagg atcgccttcg
     2461 cgcccttgct gggcgagagc gccgcgaccc acgggccggt gcgccagaag gtggcgagca
     2521 ccacgtcggc gtcgggaacg tcggcatcgc tgaccgcgcg tacgcgctcc agcacgcgat
     2581 gcggcacttc gaggccgtcg aaccaggagt gctcgggctc gggatccggc ggccagccct
     2641 gtccgcgcag cagcgacttg agcttgcgcg cgaagctcgg cacgtgctgc ggaatcgaca
     2701 ccacctgcac cgcgtggccg cgtcgctgca gccggctggc gtagattgcg agcacgcgat
     2761 tgccgcccga catgccggca tgcgggagca cgaacgtgat gcgcatgcgg cggcgctaca
     2821 ggtacttgcg cagctggcgc gccaggatcg gcggcaacag ctcgcgcgcg gcgccgcgca
     2881 gccgctgacg caggtcgggc ctgagctcct cgttggcgcg cgccctgaaa agcgtcgagt
     2941 gctcgcccga aatctcctcg gccggacggc cgttggtgaa gtagtagagc gcaagcgact
     3001 tgcgcgtcag ctcggccggg cactgcagcg ggtccggatg gccgtgatag gtgaagtcgg
     3061 tggtgccgaa gatcaccaga cgattgaaca gcgggaagat cttcgcctcg ctgcgcgtca
     3121 tgttgcggtc ccacagctcg agcgcgccgc catattcctc gcgccagtcc cggttcagat
     3181 agaccagcac gttgagccgc cggtcgagcc cgtagcgcgg atgcttgttg aagtcggcgt
     3241 gcaccgcgag cttgccgccg cgcacgatct ggtgcagacc gccgccgtcg aagcagggat
     3301 cggagatgag attctcgatc cccgtcacgc gcgacaggaa atcgaggaag gtgtaggaat
     3361 tcaggtggta cagcagcagc cgcgtgaccg gtccgaaatt cgattcgacg gccgacgcga
     3421 gcttgatctc ctgctggttg tcgaagcgtt gccacttgat cgcgccgggc cgcgggaatt
     3481 cgccgagcac cttgtcgagc agcgccgggt cgaagaagtc gtcgaacacg acgtgcggaa
     3541 atg
//
LOCUS       DASYJA010000098         1531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2375496, whole genome shotgun sequence.
ACCESSION   DASYJA010000098 DASYJA010000000
VERSION     DASYJA010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2375496"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VGO02_02130"
     CDS             complement(<1..222)
                     /locus_tag="VGO02_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEV7476516.1"
                     /translation="MKPDAALKAESAAKPSGHPDVAYGRIGVLLLNLGTPDATDYWSM
                     RRYLKEFLSDRRVIEVPRWKWWPILNLVIL"
     gene            complement(332..1312)
                     /locus_tag="VGO02_02135"
     CDS             complement(332..1312)
                     /locus_tag="VGO02_02135"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEV7476517.1"
                     /translation="MPFRFKLAEPFEEACRRIGREQIERAQIQLKSAGDPISAVHETR
                     KSLKRLRALLHLVRPALGDEAYRAENARLREVGHMLSNARDRHVLVETITKLEAVQTI
                     GRKSFAHAVRDALDSTNGGHSKTVEAATIKQAQGLLGEASRRFARLRLDGKGFEVVGA
                     GLEASYRRGRRAFRHAYVELTDEAFHEWRKDTQRHWRHMMLLSRAWSECLTARASAAR
                     SLSQVLGDDHDLALLIAFLHSDRASGLGDEAATGIEKLALHRQRELRALAQPMGQRLF
                     AEGARSLRRRMSAYWDAAVALKEHEPEEAPDGKKLTRTGARRRRTAVVRT"
BASE COUNT          254 a          503 c          505 g          269 t
ORIGIN      
        1 cagaatgacg agattgagga tcggccacca cttccagcgc ggcacctcga tgacgcgccg
       61 gtcggatagg aactccttga ggtagcggcg catcgaccaa tagtcggtgg cgtcgggggt
      121 gccgaggttc agcagcagca ccccgatgcg cccgtaggca acgtcggggt ggccactagg
      181 cttggccgcg ctttcagctt tgagcgcggc gtctggcttc atctcgttca cgcaggtcct
      241 cggggggcag gctcgcttgc gcggcagact agcgaatttg cgcgtgcgcc gcgacggtac
      301 aatgggcgcg gctgggcgcg gacgcggcag gctaggtccg aacgaccgcc gtgcgccggc
      361 gccgggcgcc ggtgcgggtg agcttcttac cgtccggagc ctcctctggc tcgtgctcct
      421 tcagcgcgac ggcggcatcc cagtatgcgg acatgcgccg gcgcaggctt cgcgcccctt
      481 ctgcaaacag ccgctgcccc atgggctgcg ccagcgcgcg cagctcccgc tggcggtgaa
      541 gcgcgagctt ctcgatgccg gtggcggcct cgtcgccaag tcccgatgcg cggtcggaat
      601 gaaggaacgc gatgagcagc gccagatcgt ggtcgtcgcc cagcacctgg gatagactgc
      661 gcgccgcgga agcacgcgcc gtgaggcatt cgctccaggc gcgcgagagc agcatcatat
      721 ggcgccagtg ccgctgcgtg tccttgcgcc actcgtggaa ggcttcatcg gtaagctcca
      781 catacgcgtg gcggaacgcg cggcgtcccc tgcgatagct tgcctctagg ccggctccga
      841 cgacctcgaa gccttttccg tcgagacgca gccgtgcaaa ccgccggctc gcttcgccga
      901 gcaagccctg ggcctgcttg atggttgccg cctcgacggt cttgctgtgt ccgccgttgg
      961 tactatcgag cgcatcacgc acagcgtgag cgaagctctt gcgaccaatc gtctgcacgg
     1021 cttcgagttt ggtgatagtc tcgaccaaaa cgtgtcggtc gcgcgcgttc gaaagcatgt
     1081 gcccaacttc gcgcagacgc gcgttctccg cgcgataggc ttcatcgccg agcgcgggac
     1141 gcacgaggtg cagcagagcg cgcaggcgct tcaacgattt gcgggtctca tgcacggcgc
     1201 tgatgggatc gcccgcgctc ttgagctgaa tctgcgctcg ctcgatctgc tcgcggccga
     1261 tgcgcctgca cgcctcctcg aacggctccg caagcttgaa gcgaaagggc atgcgcacga
     1321 ctcgtaaacg gtccgccgag aaccgagagg gccccgtgca atgtgacagc tatttgaagc
     1381 cagcaactgc gcgagcgcaa gcgccgaggc tgcggcactg gctacctttt ttcaacttgc
     1441 aaccgatcta tgacaaaacg tgcgcgtctc actggcccaa tcgagccaca cgcgtattcg
     1501 cgcctcagga tcgggcgcgg tcatgaaatc g
//
LOCUS       DASYJA010000099         2250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1106178, whole genome shotgun sequence.
ACCESSION   DASYJA010000099 DASYJA010000000
VERSION     DASYJA010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106178"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="VGO02_02140"
     CDS             complement(<1..1079)
                     /locus_tag="VGO02_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV7476518.1"
                     /translation="MKDQPSGPRLQQNIFELLVDRVSEYAIFVLDPEGRVKSWNTGAR
                     LLKGYFPEEIIGRHFSVFYPAEAVASGWPAQELKMATTQGRFEDEGWRLRKDGSRFWA
                     NVVITALRDPDGQLIGFSKITRDLTGRRQAEEDMRLSEERFRLLIEGVQDYALFMLDP
                     QGIITSWNTGAQRIKGYSREEVLGKHFSLFYGAEDVAAGKPWEELATARKTGRAEDEG
                     WRVRKDGERFWARAVLTSLYDGEGRLQGFAKVTQDLSQREYARDLENTAQRVNEFIAL
                     MAHELRNPLAPIRTAVHLMERLPAADPVQEEMRKTVERQSAHLMRIVDDMLDIARVTR
                     GTLSIQHARVDLAEVVARAVDLYRDV"
     gene            1224..1691
                     /locus_tag="VGO02_02145"
     CDS             1224..1691
                     /locus_tag="VGO02_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7476519.1"
                     /translation="MTIRARLYRDSHRLAVATAGLSSARNRARVIVIDDDPTIRELLT
                     IHLTNSGYQVLVAEDAVLGGRMIMQARPDLVICDVNMPYLDGFEFVEAMKGDPATRDI
                     PVVMLSSEDDPTGRASRLGVAAYLKKPVQAGRLLEVVALFADLAQEPTEARRS"
     gene            complement(1703..2002)
                     /locus_tag="VGO02_02150"
     CDS             complement(1703..2002)
                     /locus_tag="VGO02_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476520.1"
                     /translation="MVFKKWTFNRAAVEHAPDNRGILILWDAEEMIYVGHATGGETIK
                     TVLLRHLDGHCGECTKRATHYSWEITIWAAARETELLNEFAKKHQRDPRCQQTVA"
BASE COUNT          368 a          737 c          708 g          437 t
ORIGIN      
        1 acgtcgcgat agaggtccac cgcgcgcgcc acgacctcgg ccagatcgac cctcgcatgc
       61 tggatcgaga gcgtgccgcg ggtgaccctg gcgatgtcga gcatgtcgtc gacgatgcgc
      121 atcaggtgcg cgctctggcg ctcgaccgtc ttgcgcatct cttcctgcac cggatcggcg
      181 gccggcaggc gctccatcag gtgcaccgcg gtgcggatcg gcgcgagcgg gttgcgcagc
      241 tcgtgcgcca tcagggcgat gaattcgttg acgcgctgcg cggtgttctc caggtccctg
      301 gcgtactcgc gctggctcag gtcctgcgtc accttggcga agccttgcag ccggccctcg
      361 ccgtcgtaga gcgaggtcag caccgcgcgc gcccagaacc gctcgccgtc cttgcgcacg
      421 cgccagcctt catcctcggc ccggccggtc ttgcgcgccg tcgccagctc ctcccacggc
      481 ttgccggcgg cgacgtcctc ggcgccgtag aagagggaaa agtgcttgcc gagcacctcc
      541 tcgcggctat agcccttgat gcgctgcgcg ccggtgttcc agctggtaat gatgccctgc
      601 ggatcgagca tgaagagcgc gtagtcctgc accccttcga tcagcaggcg gaagcgctcc
      661 tcgctcaggc gcatatcctc ctccgcctgg cggcggccgg tgaggtcgcg cgtgatcttg
      721 gaaaagccga tcagctggcc gtccgggtcg cgcaacgcgg tgatgaccac gttggcccag
      781 aagcgcgagc cgtccttgcg caggcgccag ccttcgtcct cgaagcggcc ctgcgtcgtc
      841 gccatcttga gctcctgcgc cggccagccg ctcgccaccg cctcggccgg gtagaagacc
      901 gagaagtggc ggccgatgat ctcttcgggg aaataccctt tgagcaggcg cgcgccggtg
      961 ttccagctct tgacccgtcc ctccgggtcg agcacgaaaa tcgcgtactc gctcacccgg
     1021 tcgaccagca gctcgaagat attttgctgc agccgcgggc cggaaggctg gtctttcatg
     1081 gggcggtcga ttctaactgg ctcgatgggc ggccatggtc tcgcttctcc tgtagtcaac
     1141 caacgacagc gcaccgggag gttttgcgac atctgtcaag gcgagcgctt gatatattgg
     1201 aacgcgctct ccagaaaaat cgaatgacga ttcgcgctcg cctgtacagg gattctcacc
     1261 gcctcgccgt ggcgaccgcc gggctgtcgt cggcgcgcaa tcgagccagg gtcatcgtca
     1321 tcgacgatga ccccaccatc cgcgagcttc tcacgatcca tttgaccaat tcgggctatc
     1381 aggttctggt ggccgaggat gccgtgctcg gcggccgaat gatcatgcag gcgcgtccgg
     1441 acctcgttat ctgcgacgtc aacatgccgt acctcgacgg cttcgagttc gtggaagcga
     1501 tgaagggtga ccctgccaca cgagacattc ccgttgtcat gttgtcctcg gaggacgacc
     1561 cgacagggcg cgcgtccaga ctcggcgtcg ccgcctacct gaaaaagccg gtgcaggcag
     1621 gccgccttct ggaggtcgtg gcgctattcg cggatctggc tcaagagccg accgaagctc
     1681 gcagaagctg atcccgctct agtcaggcga ccgtctgctg gcagcgtgga tcgcgctgat
     1741 gcttcttggc gaactcgttg agaagctcag tctcgcgcgc cgccgcccag atcgtaattt
     1801 cccacgagta gtgcgttgca cgcttggtgc actccccgca atgtccgtcg aggtggcgaa
     1861 gcagcacggt cttgatcgtc tccccgccgg tggcgtgccc gacgtaaatc atttcctctg
     1921 cgtcccacaa gatgaggatg ccgcgattgt cgggcgcgtg ctcgaccgcc gcccggttga
     1981 atgtccattt cttgaaaacc aaggctcttc tccctgcccc ccgaaaagca gacccaatgg
     2041 taaatgcttg tcgcgcaaaa gcaggactac aggaagctcg ctgtagtgcg cccctatgag
     2101 gctttgtagc gcgagccggt gcatgtattc ctgcgcgcac tcgatctgga tcgttctcgc
     2161 cgcgcgtaag gtttgataga gctcaattca gggcgttttc cgagccgcgg cggggtggtg
     2221 gagcgggtga agggaatcga accctcgtat
//
LOCUS       DASYJA010000100         2640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_560833, whole genome shotgun sequence.
ACCESSION   DASYJA010000100 DASYJA010000000
VERSION     DASYJA010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560833"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VGO02_02155"
     CDS             complement(<1..304)
                     /locus_tag="VGO02_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEV7476521.1"
                     /translation="MNKGFVVHRSPEVARLERRLFLRRGLSLGALALLSGCNLTDQEG
                     VQRVLSAMARWNDGVQDWLFDPRRLAPEFPESRITRPFPFNAYYSEEEVIDVDGAGY"
     gene            complement(301..915)
                     /locus_tag="VGO02_02160"
     CDS             complement(301..915)
                     /locus_tag="VGO02_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEV7476522.1"
                     /translation="MEQSGWIKVHPVVVRITHWVNVVAVLLMLTSGWQIYNASPLFDF
                     SFPPALTLGGWLAGALQWHFAAMWLLVLNGLVYVVYGMVSGHFRRKLLPLSPLAVLHD
                     VREAFRGRLAHDDLSKYNAAQRAAYLALIAALAVLVASGLAIWKPVQLYWLASLMGDY
                     EGARLLHFFAMAAVVLIVVVHVVMVILVPRTFPTMFTGRARRVS"
     gene            complement(964..>2640)
                     /locus_tag="VGO02_02165"
     CDS             complement(964..>2640)
                     /locus_tag="VGO02_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEV7476523.1"
                     /translation="SRTLGSAQGRYPGWFAALFPLQAPTAESLVSGGWRQLGKVQVTV
                     HPRFAYEQLWSTARDTLIYLLFIYAAALIALRMFLRDVLRPLAAVESAAQAIAARNFV
                     TLRLRPSTRELARVVEAMNTLSRKVNEAIEAETRRAERLQDAAYRDQVTGLLNARGFA
                     ARFESSYEGEHEPFAGVFAFVELAELALINRRLGTERCDALLREVCSAMHEAAATSGG
                     FAGRWSGGLTVLALPRLGSDAAREGLAALRKEARAALKRLGLARNDKVFCGGAEVSGG
                     PAMLRHLAAAADAALMRARDSAEGIVVLRAAPPGVQAAKPASEMLALVAEALAAQRVN
                     LAGQVAYRMSDHRVLHTEIFARLRDASGREITAAEFLPAVAAQGLGEQLDRVVIGQVL
                     EAARGRADAVSVNVSIRSLEQESFMQWLLPLLRRERALAARLAFEMAENAVVQNEAAA
                     SAFARALAGTGAGFALDNFGIRRDSLALAQRLRPSYIKLAGAQTATMLANSGARFYAE
                     SLIAAARQLDIPVIAQNVEDEALFQAIAGLGFAGYQGNLGGRPSPWPPKV"
BASE COUNT          432 a          971 c          892 g          345 t
ORIGIN      
        1 ggtagccggc gccgtcgacg tcgatcacct cttcctcgct gtagtacgcg ttgaagggaa
       61 acggccgggt gatccggctt tccggaaatt cgggcgccag ccggcgcgga tcgaacagcc
      121 agtcctgcac gccgtcgttc cagcgcgcca tcgcggaaag cacgcgctgc acgccttcct
      181 gatcggtgag gttgcacccc gagagcagcg ccagcgcgcc gagcgacagc ccgcgcctga
      241 ggaacaggcg ccgctcgagg cgtgccacct ccggcgagcg atgcactacg aaacccttgt
      301 tcatgaaacc ctcctggccc ggccggtgaa catcgtcggg aacgtgcgcg gcacgaggat
      361 caccatgacc acgtgcacca cgacgatcag tacgacagcg gccatggcga aaaagtgcag
      421 caggcgcgcg ccttcgtagt cgcccatcag agatgccagc cagtacagct gcaccggctt
      481 ccagatggcc aatcccgagg cgaccagcac ggccagcgcc gcgatcagcg cgagataggc
      541 cgcgcgctgc gccgcgttgt acttcgacag atcgtcgtgc gcgagccgcc cgcggaaggc
      601 ctcgcgcacg tcgtgcagca ccgcgagcgg cgaaagcggc agcagcttgc ggcggaaatg
      661 ccccgaaacc atgccgtaga cgacgtagac caggccgttc aggaccagca gccacatcgc
      721 ggcgaaatgc cactgcagcg ccccggcgag ccagccgccg agggtcagcg cgggtggaaa
      781 ggagaagtcg aaaagcggcg acgcgttgta gatctgccag ccgctggtca gcatcaggag
      841 cacggcaacc acgttcaccc agtgcgtgat gcgcactacc acggggtgca ccttgatcca
      901 gccagactgt tccacggagc cgatttgctt cacgtcaggt aggtcgcccg ccacccgcta
      961 accttacacc ttcggcggcc acggcgacgg ccgcccgccg agattcccct ggtagccggc
     1021 aaagcccagg ccggcgatgg cctggaacag cgcctcgtct tccacgttct gcgcaatcac
     1081 cgggatgtcg agctgccgcg ccgcggcgat cagcgactcg gcatagaagc gcgccccgct
     1141 gttggccagc atggtcgcgg tctgcgcgcc cgcaagcttg atgtacgaag gcctcaggcg
     1201 ctgcgccaaa gccaggctgt cgcgccggat gccgaagttg tcgagcgcga agccggcgcc
     1261 agtgccggcg agcgcgcgcg cgaacgcgct ggcggcggcc tcgttctgca ccaccgcgtt
     1321 ctccgccatc tcgaatgcca gtcgcgcggc cagcgcccgc tcgcgccgca ggagaggcag
     1381 cagccactgc atgaagctct cctgctcgag cgagcggatc gaaacgttga cgctgaccgc
     1441 gtccgcgcgc ccccgggccg cctccagcac ctgcccgatc accacgcgat cgagttgctc
     1501 gccgaggcct tgcgcggcca cggccggcag gaactcggcg gcggtgatct cgcgacccga
     1561 ggcatcgcgc aggcgcgcga agatctccgt gtgcaggacg cggtggtcgc tcatgcggta
     1621 ggcgacctgt ccggcgaggt tgacgcgctg cgcggcgagc gcctcggcca ccagggcgag
     1681 catctcggaa gccggcttgg ccgcctgcac cccaggcggt gccgcgcgca gcacgacgat
     1741 gccctcggcg ctgtcgcgcg cccgcatcag cgcggcgtcc gccgccgccg caagatggcg
     1801 cagcatcgca ggaccgccgc tcacctccgc gccgccgcag aaaaccttgt cgttgcgcgc
     1861 cagcccgagg cgcttgagcg cggcgcgcgc ctccttgcgc agcgcagcga gcccttcgcg
     1921 cgcggcgtcg gagcccagcc gcggcaacgc gagcacggtg aggccgcccg accagcgccc
     1981 ggcgaagccg cccgaggtcg ccgcggcctc gtgcatcgcg ctgcacacct cgcgcagcag
     2041 cgcgtcgcag cgctcggttc ccagcctgcg gttgatgagc gcgagctccg ccagctcgac
     2101 gaaggcgaag acgcccgcga aaggctcgtg ctccccttcg tagctgctct cgaagcgcgc
     2161 ggcgaagccg cgcgcgttga gcaggccggt cacctggtcg cggtaggccg cgtcctgcag
     2221 cctctcggcg cgccgcgtct cggcttcgat cgcctcgttc accttgcgcg acagcgtgtt
     2281 catcgcctcg actacccgcg ccagctcgcg cgtcgacggg cgcagccgca gcgtgacgaa
     2341 gttgcgcgcc gcgatcgcct gcgccgcgct ttccaccgcg gccagcggcc gcagcacgtc
     2401 gcgcaggaac atgcgcagcg cgatgagcgc ggcggcatag atgaagagga gatagatgag
     2461 cgtgtcgcgc gccgtgctcc acagctgctc gtaggcgaag cgcgggtgca ccgtgacctg
     2521 caccttgccg agctggcgcc agccgccgct caccagcgac tccgcggtcg gcgcctgcag
     2581 cgggaacagc gccgcgaacc agcccgggta gcggccctgc gccgagccca gggtgcggct
//
LOCUS       DASYJA010000101         4374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_924664, whole genome shotgun sequence.
ACCESSION   DASYJA010000101 DASYJA010000000
VERSION     DASYJA010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924664"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VGO02_02170"
     CDS             <1..748
                     /locus_tag="VGO02_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476524.1"
                     /translation="SDETNAAQLHPLTPQSAAMMLGKAGLGRPWPANAGPHSLAAGQR
                     LFHLGIGTRPLTVPGSGRRPRLRRRLHIKVTLDRPQDHVRVCVFVSEVKAQKLAQRLR
                     DASALGSVSTAFQRWMGRRLERIFLHAAHGRLRVVDAGLRPGASTDAALANLPLAVRQ
                     AFMAKLHEWLVRGFADFAKTQAQRFLAATADAADGVTLRFVVEHPQGLKDFVRGLVER
                     GAPASAVAATISGAAPPTVRVDVFPGHRCA"
     gene            741..2009
                     /locus_tag="VGO02_02175"
     CDS             741..2009
                     /locus_tag="VGO02_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476525.1"
                     /translation="MPDLLAAVDIQTERQVAHWTLAAARLDLDELASPEAWARLERYL
                     GIALRRHLQGVIDRLASDARVLTTLQREARSLTAHAEVRRRLLAFRAQYLRAETTLDF
                     FADAINVRTNVRTGALLRACDTLAHRSMSQLLEQIGKTAPVSLTYVDKGLGASILKAG
                     LRLWDGGDACPVAVIKITRHNLLRPTALIHEAGHQVAHICGWTGELATAFARGLPLEA
                     GASSELANIWAAWTSEIAADAFAFVHTGFASVAALHDVLAGDAAGAFRHIAGDPHPIS
                     HLRVLLAVQMCRHCYGSGPWDGLALAWNHLHPLERANAGVRAIVQDSMPLLDAVVRLT
                     LDAPMQAFGGRALRKLIAPERVSPSALAELEGRIGPALFTSAHWLWAEPLRILGLTGL
                     QLAQSPDRLASILDLQQQSMLRLGGALQAA"
     gene            2043..2339
                     /locus_tag="VGO02_02180"
     CDS             2043..2339
                     /locus_tag="VGO02_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476526.1"
                     /translation="MASNPNPSSSPSSSATRDPYPAPGPAPSPTDVTALLLEVLRNVN
                     REVKTTLDLSGRAQAAVDHFRAISVSLRTLADGSAFFNASTGTAAGTTPTPRPR"
     gene            2403..3521
                     /locus_tag="VGO02_02185"
     CDS             2403..3521
                     /locus_tag="VGO02_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476527.1"
                     /translation="MFRQFATLVSPDALFDAHPFELAWLLEEVWRNPITTAGKVLGDP
                     DRRSNLQATPPYTDEWPPVSAALEPILAPVQTDPTVLRTYRWDHLIYAYMIENSRVYE
                     IFRRVMHEFLHGEKLGAPTEESQRWLRNTEELFYNDGAPFFIGSVTSRIRSDLRATRR
                     NPYQRMFAMELNHGGEDGKPYPYVHAEAANNEFVSTFEEFLREVWVAMTFVTATSSSN
                     PTDPSKIATLATKLKNMLMSRRQSGNLAREEFAAVSAMAWFHLTLEYKNNGEHAPIVR
                     ALRAEGDGTEQRLFKIAERVGLPAHGLSKHFFDIADAISRILIQIESGDYDFADAVTA
                     LYTPASGGNTPETAMRNIITNWTAITGRDVKARKVTPS"
     gene            3528..>4374
                     /locus_tag="VGO02_02190"
     CDS             3528..>4374
                     /locus_tag="VGO02_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV7476528.1"
                     /translation="MNTALESPFLDAESEVGVAEAPWQALRLDSPYARETPGREALLE
                     EEKGIIDGDDRQAVADPLAVPFRWICSIAVVREITHASGRTSRTGLAPAGTGVLISPR
                     HVLTAAHVVKSIRRDSSNSVSERHEARTVRVKFGRDASGGALVEVEAERWDWPRAQWK
                     PELDNSSFDFALLTLKTAVGTQVFKALGNQALGHWGVDAKHSLDNLPSALAARMLGAR
                     VVTAGYPESGKGQMMCASGLLSTGQPANDRALGTPALTEQWIRGGALFSMTADATEGQ
                     SGSPVW"
BASE COUNT          665 a         1599 c         1476 g          634 t
ORIGIN      
        1 ctccgacgag accaatgccg cgcagctcca tccgctgacg ccgcagtcgg cggcgatgat
       61 gctcggcaag gccggcctgg gccggccatg gccggcgaac gcggggccgc acagcctggc
      121 cgccggacag cgtctctttc atctcggcat cggcacgcgc ccgctcaccg tgccgggctc
      181 cggtcggcga ccacgcttgc gccgtcgcct gcacatcaag gtcacgctgg accggccgca
      241 ggaccacgtg cgcgtctgcg tcttcgtgag cgaggtcaag gcgcagaagc tcgcccagcg
      301 cctgcgcgac gcttccgccc tcgggtccgt ctcgaccgcg ttccagcgct ggatggggcg
      361 acggctcgag cgcatcttcc tccacgccgc gcacgggcgt ctgcgcgtcg tcgatgccgg
      421 gctgcgcccc ggcgcgagca ccgacgcggc gctcgccaac ctgccgctgg cggtgcgcca
      481 ggcgttcatg gcgaagctgc acgagtggct tgtgcgcggc ttcgccgact tcgcgaagac
      541 ccaggcgcag cgcttcctcg ctgcgaccgc agatgccgcc gacggcgtga cgctgcgctt
      601 cgtcgtcgag catccgcagg gcctgaagga cttcgtgcgg gggctcgtcg agcgcggcgc
      661 gccggccagt gcggtggcgg ccacgatctc cggcgcggcg ccgccgacgg tgcgtgtcga
      721 cgtcttcccg gggcaccgct gtgcctgacc tgctcgccgc cgtcgacatc cagaccgagc
      781 gccaggtcgc gcactggacg ctcgcggccg cgcgcctcga tctagacgag ctcgcctcgc
      841 ccgaggcctg ggcccggctg gagcgctatc tcggcattgc gctgcggcgc catctgcagg
      901 gagtcatcga ccgccttgcg agcgacgccc gggttctgac gacgttgcag cgcgaagcgc
      961 gcagcctgac ggcgcacgcc gaggtgcggc ggcgcctgct tgcgttccgc gcccagtacc
     1021 tgcgcgccga gacgacgctc gacttcttcg ccgatgcgat caacgtccgc accaacgtgc
     1081 ggaccggcgc gttgctgcgc gcctgcgaca cgctggccca ccgcagcatg tcgcagctgc
     1141 tcgagcagat cggcaagacc gcgccggtct cgctgaccta tgtcgacaag ggcctcggcg
     1201 cgtccatcct gaaggccggg ctgcggctgt gggacggcgg cgatgcctgt ccggtcgcgg
     1261 tcatcaagat cacgcgccac aacctgctcc ggccgaccgc gttgatccac gaggccgggc
     1321 atcaggtcgc gcacatctgc ggctggaccg gcgagctcgc caccgcgttc gcgcgcggac
     1381 tgccgctcga ggccggcgcg tcgagcgagc tcgcgaacat ttgggctgcc tggacctcgg
     1441 agatcgcggc cgatgcgttc gccttcgtcc acaccggctt cgcgagcgtg gccgcactgc
     1501 acgacgtgct ggccggcgac gccgccggcg cgttccgcca catcgcgggc gaccctcatc
     1561 cgatcagcca cttgcgcgtg ctgctggcgg tccagatgtg ccgccactgc tacggaagcg
     1621 ggccgtggga cgggctcgcg ctggcctgga accacctgca tccgctggag cgcgcgaacg
     1681 ccggggtgcg cgcgatcgtg caggactcga tgcccttgct cgacgctgtc gtgcggctca
     1741 ccctcgatgc gccgatgcag gcgttcggcg gtcgcgcgct gcgcaagttg atcgcgccgg
     1801 agcgcgtgag cccgagcgcg ctcgccgagc tcgaaggtcg cattggcccg gcgctcttca
     1861 cgtcggcgca ttggctctgg gccgagccgt tgcgcatcct cggcctcacc gggctgcagc
     1921 tcgcgcagtc cccggaccgt ctcgcgtcca tcctcgatct gcagcagcag tcgatgctgc
     1981 gcctcggcgg cgcgctgcaa gcagcctgat cacgccgctt caacgtcaac taggagggcc
     2041 ccatggccag caacccgaac ccgtcttcgt caccgtcatc gtccgcaacg cgcgatccgt
     2101 acccggcacc gggtcccgcg ccgagtccga ccgacgtcac cgcgctcctg ctcgaggtgc
     2161 tgcgcaacgt gaaccgcgag gtcaagacga cgctcgatct ttcgggccgg gcgcaggccg
     2221 ccgtcgacca cttccgcgcg atctcggtca gtctgcgcac gctggccgac gggagtgcct
     2281 tcttcaacgc cagcaccggc actgctgcgg gcacgacgcc caccccgcgg ccgcgctgag
     2341 cggccgaccg ccgagccggc ccatccatcg tcccgcaagc gtccgcgcaa ggagaagctt
     2401 ccatgttccg ccaattcgca accctggtct cacccgatgc gctgttcgac gcgcacccgt
     2461 tcgagctggc gtggctgctc gaggaggtct ggcgcaatcc gatcaccacc gccggcaaag
     2521 tgctcggtga cccggaccgg cgcagcaacc tgcaggcgac gccgccctac accgacgagt
     2581 ggccgccggt cagcgccgcg ctggagccga tcctcgcgcc ggtgcagacc gatccgacgg
     2641 tgctgcggac ctaccgctgg gaccacctga tctacgccta catgatcgag aactcgcgcg
     2701 tctacgagat cttccgccgc gtgatgcacg agttccttca cggcgagaag ctcggcgcac
     2761 ccacagaaga aagccagcgc tggctgcgca acacggagga gctgttctac aacgacggcg
     2821 cgccgttctt catcggctcg gtcaccagcc gcatccgcag cgatctgcgc gccacgcgcc
     2881 gcaatccgta ccagcgcatg ttcgcgatgg agctcaacca cggcggcgag gacggcaagc
     2941 cgtaccccta cgtacatgcc gaggcagcca acaacgagtt cgtctcgacc ttcgaggagt
     3001 tcctgcgcga ggtctgggtc gccatgacgt tcgtgaccgc gacgtcgagc agcaacccga
     3061 ccgatccgtc gaagatcgcg accctcgcga ccaagctgaa gaacatgctg atgtcgcggc
     3121 gccagagcgg caacctcgcg cgcgaggagt tcgcggcggt gagcgcgatg gcctggttcc
     3181 acctcaccct cgagtacaag aacaacggcg agcacgcgcc gatcgtgcgg gcgctgcgcg
     3241 ccgaaggcga cggcaccgaa cagcgcctgt tcaagatcgc cgaacgggtc ggcctgccgg
     3301 cgcacggcct gtcgaagcac ttctttgaca tcgccgatgc gatctcgcgc atcctgatcc
     3361 agatcgagag cggcgactac gacttcgcgg acgcggtgac cgccctgtac acgcccgcgt
     3421 ccggcggcaa tacgccggag acggcgatgc gcaacatcat caccaactgg accgcgatca
     3481 ccggccgcga cgtgaaggcg cgcaaggtca cgccgagctg agcgagcatg aacaccgcgc
     3541 tcgaatcgcc gttcctcgac gccgagtccg aggtcggcgt ggcggaggcg ccctggcagg
     3601 cgctccgcct cgacagcccg tatgcgcgcg agacgccggg ccgcgaggcg ctgctcgagg
     3661 aagagaaggg gatcatcgac ggcgacgatc gccaggccgt cgccgacccg ctggccgtgc
     3721 cgttccgttg gatctgcagc atcgccgtcg ttcgcgagat cacccacgcc tccggccgta
     3781 ccagccgcac cggccttgcg ccggcgggca ccggcgtgct gatctcgccg cgccacgtcc
     3841 tgactgcggc gcacgtggtg aagagcatca ggcgcgacag cagcaacagc gtcagcgagc
     3901 ggcacgaggc gcggacggtg cgcgtcaagt tcggccgcga cgcgagcggc ggagcgctcg
     3961 tcgaagtgga agccgagcgt tgggattggc cgcgtgcgca gtggaaaccg gagctggaca
     4021 actcctcatt cgacttcgcg ctcctcacgc tgaagactgc ggtcggtacg caggtcttca
     4081 aggcgctcgg caaccaggcg ctcggccact ggggcgtcga tgcgaagcac tcgctcgaca
     4141 acctgccgtc ggcgctggcg gcacggatgc tcggtgcacg cgtcgtcacc gcggggtatc
     4201 cagaaagcgg caagggccag atgatgtgcg cctcgggcct gctctcgacc ggccagccgg
     4261 cgaacgatcg ggcgctcggc acgccggcgc tgaccgagca atggatccga ggcggcgcgc
     4321 tcttttcgat gactgccgac gccaccgagg gccagagcgg ctcgccggtg tggg
//
LOCUS       DASYJA010000102         6955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1560322, whole genome shotgun sequence.
ACCESSION   DASYJA010000102 DASYJA010000000
VERSION     DASYJA010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6955
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560322"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VGO02_02195"
     CDS             <1..187
                     /locus_tag="VGO02_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEV7476529.1"
                     /translation="DAACQIQIDALAGGLHNVQIMSEQAAETAYGQFHRANRPGGYKD
                     WPALLRMLERKGIKYAE"
     gene            complement(171..1358)
                     /locus_tag="VGO02_02200"
     CDS             complement(171..1358)
                     /locus_tag="VGO02_02200"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7476530.1"
                     /translation="MPRTGDALVIGLVALAHGLSHFFQLIIAPLFPLIKEELGVSYAA
                     LGFVVMLFYTLSALFQPVAGFVVDRFGGRAVLLGGVGCLTLGTLIAGAAPSYGVLVAG
                     AALAGMGNSVFHPADFSILNARVGVQRLGYAYSAHGIAGSLGYASAPIFSGGLGTLIG
                     WHNALFAATGVGAFAFLLLLTNWNRLHMAEAPGKRPEGKTEFRILFAAPVVMCFLYFA
                     IYAAGLAGLQSFSVSAMTVQYGVAAALASSALTAYMIGSAAGIFAGGFIATRTTRHDL
                     VAAAGLALSASVVSIIAISAVPGTALPAMLALAGFCVGATAPSRDMIVRASTPAGATG
                     RVYGFVYSGLDVGSLSTPVFYGWLMDHNLPQGVFYTVAGLTLLAMFTVLQLPGTKRAI
                     QRI"
     gene            complement(1342..2100)
                     /locus_tag="VGO02_02205"
     CDS             complement(1342..2100)
                     /locus_tag="VGO02_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7476531.1"
                     /translation="MPSVQAAGRSLNYEWVGSAGPHTLVFLHEGLGSIRQWRDFPAKV
                     SAATGCRALVYDRYGYGASDVLSESRRNVHFMHDEALRALPEMLKELKIEKPILVGHS
                     DGASIALIHAGGGFAVKAVVAMAPHVFIEPLCLKSIDRAAESFEKGDLAKGLGKYHRD
                     ARKTFYGWADVWLGPDFTSWDIRDDYLPRIACPVLAIQGEDDEYGTMAQLEDMKRRVK
                     RCELLKLPDCGHSPFKDQPEKVLSAIQSFIAKNR"
     gene            2136..3029
                     /locus_tag="VGO02_02210"
     CDS             2136..3029
                     /locus_tag="VGO02_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV7476532.1"
                     /translation="MRFTAWLVLFATLLLWSGNWIVARAVRDEVAPGVATVGRLVVVL
                     AVLLPFVVFSLKQKLKTLSRRGWTILAAMGFAGGGLHLALQWLGLHFTTATSGILYLS
                     TAPIFVLLMAVPLLGERIGRRQWAGVAISFTGVATIAAQGEMAHLAALSFNKGDMMAL
                     ASMAMWAAYTVLLRLRRDPLDVAELIVMVCAFGVLFMLPWLAVERAPLRLSETGLLAI
                     LYSAIASLLLAYAGWSYAVTRLGAVRAGVTMHLMPAMGVVLAAIFLAEYPRWYHFAGI
                     ALVLAGVGLSSFRASSASSTR"
     gene            complement(2998..3984)
                     /locus_tag="VGO02_02215"
     CDS             complement(2998..3984)
                     /locus_tag="VGO02_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid 1,2-hydratase"
                     /protein_id="HEV7476533.1"
                     /translation="MNRRRFIFSPLLIYPGIAFAEKATYPTVRPNPKFQFPRDHGAHP
                     EYRIEWWYVTGWLDGPLGFQITFFRARPEEQSANPSSFNPRQVLFAHATLSDPKRGRL
                     LHDQRAARAGFSLAHAEIDRTGVWIDDWKLELEANRYHARIAARDFDFDFTLFSPQVV
                     LQGEQGFSRKGRRPGEASYYYSRPQINVVGSVNGRQVTGTAWLDHEWSSAYLAPEAVG
                     WDWCGINMLDGSSLMAFQMRDKKGGIHYASPGATMRPLRIWKSPRTGTRYPVSMQVND
                     LRLEPLMDDQELDSRPTAGTIYWEGAVTAYKDDAAVGRGYLELTGYWKPMKL"
     gene            complement(3981..6401)
                     /locus_tag="VGO02_02220"
     CDS             complement(3981..6401)
                     /locus_tag="VGO02_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7476534.1"
                     /translation="MLELLRTLGARRARGRAILSILGIALGVALGYGVSLVNRAAVED
                     LEAAVRTLSGEADLQVRGGRSGFAESLYPRIARLAGVASVSPGLELEAGIAGSERTIR
                     VVGVDAVRQGRIELLKPDRAILSPAAAALVKDRLPLVVGDKTVTLTVADVDASLKGMV
                     ALTDVATAQWRLARLGELNRLDVFLKNGFQKEGVLAQIEALLPPGVHASPIESAEQAS
                     AYPSRAYRVNLNVLAMVALFTGGFLVFSAQALEVTRRRGEHALLRVLGLRRGGLARLV
                     LLEAAALGAAGGLLGVALGHALAIVMLRVWGADFGAGAFRTFPAAHFSVLPALLFLLA
                     GIGIALAGALLPALDAARAAPARALKAGDEQAMLGRLFPLWPGVALLAAGAGLAQLGP
                     VQGLPLFGYAAIGCLLMGSILLVPFLFSLLLDRITFLKTPALVLAVAQLRGAPGQAAV
                     SLAAIVASFALMAAMAIMVASFRESLERWLEAVLPAELYFRTTHAGDTGYLEPQFEAR
                     VRALPQVARAEFLRSARLLLAPNRPAVVLLARDRAAPALPVGRQYERRAGDPPPAWIS
                     EAVFDLYGFSPGSRIQLPMSNKSLQFVVAGVFRDYARQHGAIQIDRADYVALTGDRRV
                     NDAALWLAPGATPAAAMEAIRALPGASELDLALPGEIREVSLRIFDRSFAATYAMEAV
                     AVLVGLFGLSSSLGAIVLARRREFGVLRHLGLTRAQIGAMLAAEGGLLALLGAAAGLA
                     AGGAISLVLVHVVNRQSFNWSMELHLPWGLLGLLVFILVALAVLTAILSGKEAMGMGP
                     VRAVREDW"
     gene            6419..>6955
                     /locus_tag="VGO02_02225"
     CDS             6419..>6955
                     /locus_tag="VGO02_02225"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV7476535.1"
                     /translation="MRWLLALTLICSEALAQQPPAWFTESLFFVREDAAEAAKEGKRV
                     MLYFGQDGCPYCKQLMEVNFRQARIVEKMNRSFVAIALNIWGDREITWSDGRVMSEKQ
                     LAVALKVQFTPTLLFLDEKGEIALRVNGYLPPERFEAALDYVTGKMEGKMAFADFRSS
                     AQPASAKLNEQPFFMKPPY"
BASE COUNT         1194 a         2356 c         2363 g         1042 t
ORIGIN      
        1 agacgcggcc tgccagatcc agatcgacgc gctggccggc ggcctgcaca acgtgcagat
       61 catgtcggag caggccgcgg agactgccta cggccagttc catcgggcca accgcccggg
      121 cggctacaag gactggccgg cgcttcttag gatgctggaa cgcaagggaa tcaaatacgc
      181 tgaatagcgc gtttcgttcc aggtagttgt aatacggtaa acatcgcgag gagcgtcagc
      241 cccgctaccg tgtagaacac tccctgcggc aggttgtggt ccatcagcca gccatagaac
      301 accggcgtcg acagcgagcc gacgtcgagc cccgagtaaa cgaacccata gaccctgccc
      361 gtcgccccgg ccggcgtcga ggcgcgcacg atcatgtcgc gcgacggcgc ggtggcgccg
      421 acgcagaagc cggcgagcgc cagcatcgcc ggcagcgcgg tgccgggcac cgcgctgatc
      481 gcgatgatcg acaccacgct ggcgctgagc gccaggccgg cggccgccac caggtcgtga
      541 cgcgtcgtgc gcgtggcgat gaagccgccg gcgaagatgc cggccgccga gccgatcatg
      601 tacgcggtga gcgcgctcga ggcgagcgcc gccgcgacgc cgtactgcac cgtcatcgcc
      661 gagacgctga agctctgcag cccggccagg ccggcggcgt agatggcgaa gtagaggaag
      721 cacatcacta ccggggctgc aaaaagaatt cggaactccg tcttgccctc cggacgcttt
      781 cccggggctt ccgccatgtg caggcgattc cagttcgtca gcagcaggag gaaagcgaag
      841 gcgcctaccc ctgtcgcggc gaaaagcgcg ttatgccagc cgatcagcgt accgagaccg
      901 ccgctgaaga tcggcgccga ggcgtagccg agcgagccgg cgataccgtg cgcgctgtac
      961 gcgtagccga ggcgctggac gccgacgcgc gcattgagga tcgagaagtc cgccgggtga
     1021 aacacgctgt tacccatgcc ggcgagcgcc gcccccgcca ccagcacgcc gtagctcggc
     1081 gcggcgccgg cgataagcgt gccgagcgtg aggcagccga cgccgccgag cagcaccgcg
     1141 cgcccgccga agcggtcgac gacgaagccg gcgaccggct ggaacagcgc cgacagcgtg
     1201 tagaacagca tcacgacgaa gccgagcgca gcgtacgaca cgccgagctc ctccttgatc
     1261 agcggaaaca gcggcgcgat gatcagctgg aagaagtggc tcaggccgtg cgccagcgcc
     1321 accaggccga tgactagcgc gtcaccggtt cttggcaatg aaggattgga ttgcagacag
     1381 cactttctcg ggttggtcct tgaacgggct gtgcccgcaa tcgggcagct tcagcagctc
     1441 gcagcgcttg acccttcgct tcatatcctc gagctgcgcc atggtgccgt actcgtcgtc
     1501 ctcgccctgg atcgccagga ccgggcaggc gatccgcggc aggtagtcgt cgcggatgtc
     1561 ccagctcgtg aaatcggggc cgagccagac gtcggcccag ccgtaaaagg tcttgcgcgc
     1621 atcgcgatga tacttgccga gccctttcgc caggtcgccc ttctcgaaac tctccgccgc
     1681 ccggtcgatc gatttcaggc acagcggctc gatgaacaca tgcggcgcca tcgccaccac
     1741 cgccttcacg gcgaaccctc cgccggcgtg gatcagcgcg atggacgcac catcggagtg
     1801 tcctaccagg atcggtttct cgatcttcaa ctccttgagc atctccggaa gggcccgcag
     1861 ggcttcatcg tgcatgaaat gcacgttacg cctggattca ctgaggacat ccgaagcgcc
     1921 gtatccatag cggtcataca ccaaggccct gcagccggtg gccgctgaaa ccttcgccgg
     1981 gaagtcgcgc cactggcgga tcgagccgag gccttcgtgc aggaacacca gcgtgtgcgg
     2041 ccctgcgctg cccacccatt cgtagttgag cgagcggccg gctgcttgca ccgagggcat
     2101 gaggcgaatc tagcacgcgc gcgataatgc gctcgatgcg tttcaccgcc tggctggtgc
     2161 tgttcgcgac gttgctcctg tggtcgggca actggattgt cgcgcgcgcc gtgcgggacg
     2221 aggtcgcgcc gggcgtcgcc actgtaggca ggctcgtcgt agtgctcgcg gtcctgttgc
     2281 cgttcgtcgt tttttccttg aaacagaaac taaaaacatt gagccgcagg ggctggacaa
     2341 tcctcgccgc catgggcttc gctggcggcg gcctgcacct cgcgctgcag tggctcggcc
     2401 tgcatttcac gaccgccacc agcggcatcc tgtacctgtc gacggcgccg atcttcgtgc
     2461 tgctgatggc ggtaccgctg ctcggcgaac gcatcggccg gcggcagtgg gcgggcgtgg
     2521 cgatctcgtt cacgggcgtc gccaccatcg ctgcgcaggg cgagatggcg cacctcgcag
     2581 cgctgtcctt caacaagggc gacatgatgg cgctcgcctc gatggcgatg tgggccgcct
     2641 acaccgtgct gctgcgcctg cggcgcgacc cgctcgacgt agccgagctg atcgtgatgg
     2701 tgtgcgcctt cggcgttctc ttcatgctgc cctggctcgc cgtcgagcgc gcgccgctgc
     2761 ggctgagcga gaccggcctg ctcgccatcc tgtattcggc gatcgcctcg ctgcttctcg
     2821 cgtatgccgg ctggagctac gcagtcacgc gccttggcgc cgtgcgcgcc ggcgtgacca
     2881 tgcacctgat gccggcgatg ggcgtagtgc tggcggcgat cttccttgcc gagtatccgc
     2941 gctggtacca cttcgccggc atcgcgctgg tcctcgccgg cgtcgggctg tcctcgttca
     3001 gagcttcatc ggcttccagt acccggtgag ctcgaggtaa ccccggccga ctgccgcatc
     3061 gtctttgtat gcggtgaccg cgccttccca atagatcgtc cctgcggtgg ggcgcgaatc
     3121 gagctcctgg tcgtccatca acggctcaag tcttaggtcg ttcacctgca tggacacggg
     3181 ataccgggtg cccgtacggg gagacttcca gatgcgaagc ggccgcatcg tagcgccggg
     3241 agatgcatag tggatgccgc cctttttgtc gcgcatctga aaagccatca atgaagagcc
     3301 gtcgagcatg ttgatgccgc accagtccca gccgacggcc tcgggcgcga ggtaggcgct
     3361 ggaccattcg tggtcgagcc aggcggtgcc ggtgacctgc cggccgttga ccgagccgac
     3421 gacgttgatc tgcgggcggc tgtagtagta gctcgcttcc ccggggcggc ggcccttgcg
     3481 gctgaatccc tgctcgccct gcagcaccac ctggggcgag aacagggtga agtcgaagtc
     3541 gaagtcgcgc gccgcgatgc gcgcgtggta gcggttcgcc tcgagctcga gcttccagtc
     3601 gtcgatccag accccggtgc ggtcgatctc ggcgtgcgcg agggagaatc cggcgcgcgc
     3661 cgcgcgctgg tcgtgcagca gccggccgcg cttcgggtcg gacagcgtgg cgtgcgcaaa
     3721 gaggacctgg cgcgggttga agctgctcgg attggcgctc tgctcctccg gccgcgcgcg
     3781 gaagaacgtg atctggaagc cgagcgggcc gtcgagccag ccggtgacgt accaccactc
     3841 gatgcggtac tcgggatggg cgccgtggtc gcggggaaac tggaacttcg ggttcggcct
     3901 taccgtcggg taggtcgcct tctccgcaaa agcgattccc ggatagatca ggagaggtga
     3961 aaaaataaac cgccttctgt tcaccaatcc tcccgtaccg cgcgcaccgg acccatcccc
     4021 atcgcttcct tgcccgagag gatggcggtc aggaccgcca gcgcgaccag gatgaagacg
     4081 agaagtccca gcagacccca gggcaggtgc agctccatgc tccagttgaa cgactggcgg
     4141 ttgacgacgt gcaccaggac caggctgatc gcgccgcccg cggcgaggcc ggcggccgcg
     4201 cccagcagcg cgagcaggcc gccctcggcg gcgagcatgg cgccgatctg cgcgcgcgtc
     4261 aggccgaggt ggcgcagcac gccgaactcc cggcgccgtg cgagcacgat ggcgcccagg
     4321 ctggaggaga ggccgaacag gccgaccagc accgcgacgg cctccatcgc gtaggtggcg
     4381 gcgaagctgc ggtcgaagat gcgcagcgag acctcgcgga tctccccggg cagcgcgagg
     4441 tcgagctcgc tggcgccggg cagcgcacgg atcgcctcca tggccgccgc cggcgtcgcg
     4501 cccggcgcca gccatagcgc ggcgtcgttc acgcgccggt cgccggtcag cgcgacgtag
     4561 tcggcgcggt cgatctggat ggcgccgtgc tggcgcgcgt agtcgcggaa cacgccggct
     4621 accacgaatt gcaaactctt gttgctcatc ggcagctgaa ttcgtgagcc cggagagaag
     4681 ccatagagat caaagacggc ttcgctgatc cacgccggcg gcggatcgcc cgcgcgccgc
     4741 tcgtattgcc ggccgaccgg caaggccggc gcggcgcggt cgcgcgcgag gagcaccacc
     4801 gcaggccggt tcggcgcgag gagcagtcgc gcgctgcgca ggaattcagc gcgcgccacc
     4861 tgcggcagcg cgcgcacccg cgcctcgaac tgcggctcga ggtagccggt gtcgccggca
     4921 tgcgtggtgc ggaagtacag ctcggccggc agcaccgcct cgagccagcg ctcgagcgac
     4981 tcgcggaacg aggccaccat gatggccatc gccgccatca gcgcgaagct cgccacaatc
     5041 gccgcgaggc tcaccgccgc ctgcccgggc gcgcctcgca gctgagcgac cgcgaggacc
     5101 agcgccgggg ttttcaggaa agtgatcctg tccagcagaa gcgaaaacag aaacggcacg
     5161 agaagaatgc ttcccatcag caggcagccg atggcggcat agccgaagag cggcaggccc
     5221 tgcaccggcc cgagctgcgc caggccggcg ccggcggcga gcagcgctac gcccggccac
     5281 aacgggaata ggcgcccgag catcgcctgc tcgtcgccgg ccttgagggc gcgcgccggc
     5341 gcggcgcggg cggcgtcgag cgcgggaagc agcgcgccgg cgagcgcgat gccgatgccg
     5401 gcgaggagga agagcagcgc gggaagcacg gagaaatgcg ctgcaggaaa cgtgcggaat
     5461 gcgccggcgc caaagtccgc gccccatacg cgcagcatca caatggcgag cgcgtggccg
     5521 agcgcgacac cgagcagccc gcccgccgcg ccgagcgccg ccgcctcgag cagcaccagc
     5581 cgcgcgaggc cgccgcgccg caggcccagc acgcgcagca acgcatgctc gccgcgccgc
     5641 cgcgtgacct ccagcgcctg cgcggagaac accaggaagc cgccggtaaa gagcgccacc
     5701 atcgcgagca cgttgagatt gacgcggtag gcgcgcgacg gataggcgct cgcctgctcg
     5761 gcgctctcga tgggcgaggc gtggacgccc ggcggcagga gggcttcgat ttgcgcaaga
     5821 accccttcct tctgaaaacc attcttgagg aaaacgtcca ggcgatttag ctcgccaagc
     5881 cgcgcgaggc gccactgcgc ggtggcgacg tcggtcagcg cgaccatccc cttcagcgaa
     5941 gcgtcgacgt ccgcaacggt cagcgtcacg gttttgtcgc cgaccaccag cgggagccgg
     6001 tccttcacca gggcggctgc agccggggaa aggatcgcgc gatcgggctt gagcagctcg
     6061 atgcgcccct ggcgcacggc gtcgacgccg acgacgcgga tcgtgcgctc gctgccggcg
     6121 atgccggcct cgagctccag ccccgggctg accgatgcca cgccagccag gcgcgcgata
     6181 cgcgggtaga gcgactcggc aaagccgctt cgtccgccgc gcacctgcag gtccgcctcg
     6241 ccggaaagcg tgcgcaccgc cgcctcaaga tcctccaccg cggcgcggtt caccagcgac
     6301 acgccatagc cgagcgccac gccgagcgcg atgcccagta tcgagagtat tgcccttccc
     6361 cgggcccgtc tcgcgcctag cgtgcggagc agttccagca tggggctatt attgccccat
     6421 gcgatggctt ttagcgctga ctctgatttg cagtgaagcg ttggcgcagc agccgccggc
     6481 ctggttcacc gagtcgcttt tcttcgtgcg cgaggacgcg gccgaggcgg cgaaggaagg
     6541 caagcgcgtc atgctgtact tcggccagga cggctgcccc tactgcaagc agctgatgga
     6601 ggtgaatttc cgccaggcgc gcatcgtcga gaagatgaac aggagctttg tggcgatcgc
     6661 gctcaatatc tggggcgacc gggaaatcac ctggagcgat ggccgtgtga tgagcgaaaa
     6721 gcaactcgcc gttgcgctca aggtgcagtt cacccccacc ctcctcttcc tcgatgagaa
     6781 aggcgagatc gcgctgcgcg tcaacggcta cctgccgccc gagcgcttcg aggccgcgct
     6841 cgactacgtg acggggaaga tggaagggaa gatggcgttc gcggatttcc gcagcagcgc
     6901 gcagcccgca agcgcgaaat tgaacgaaca gccattcttc atgaagcccc cttac
//
LOCUS       DASYJA010000103         4278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1288026, whole genome shotgun sequence.
ACCESSION   DASYJA010000103 DASYJA010000000
VERSION     DASYJA010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288026"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1390
                     /locus_tag="VGO02_02230"
     CDS             <1..1390
                     /locus_tag="VGO02_02230"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEV7476536.1"
                     /translation="ARSHVLLWLALATTGSVLLLAVTNHLTQNVASVPLLWLAPLTIY
                     LLSFILTFEGQGWYRPRWMWPVVLAALGVMVWMLVDERYHYELALQLGVFLPGLLIGC
                     FFCHGELYRLRPGAERLTTFYLTVAAGGAIGGLLVAVAAPLAFNGYYELGAALIALAT
                     LAVVRFSAINLAARIASLALLVGAWAGAVYDGFTDKKDVLAASRSFYGVLRVKEYGEP
                     GQESHLRRLVHGTIMHGEQHMYGELRRRPTTYYEETSGVGAAIESRQDKPMRVGVIGL
                     GTGTIAAYGRAGDFYKFYEIDPDVVRVASSDFTYLSDSPAKVEVALGDARLTLEREAP
                     QRFDVLAIDAFSSDAIPVHLITREALAVYLKHAGADGIVAFHVSNRFLDLIPVVARIA
                     RENGAHAVLVRDDPDEDEDHRSRTDWVLVSRDPKALERAAIVKASAEPAKDRPEWRTW
                     TDDYSNLVQILR"
     gene            complement(1419..2363)
                     /gene="secF"
                     /locus_tag="VGO02_02235"
     CDS             complement(1419..2363)
                     /gene="secF"
                     /locus_tag="VGO02_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEV7476537.1"
                     /translation="MEFFKIRGTIPFMRHALVFNVISLITFLAAVFFLATRGLHFSVE
                     FTGGTLMEVSYQHPAEVERVRDSLDKAGFKAEVQNFGSSRDVLIRLPVIKDVPGAEVS
                     QKITALLQADDKSAEVRRVEFVGPQVGGELAEMGAVALLLVAVAIVAYLWLRFVNWRF
                     GLAAIVANLHDVVIILGFFAFFQWEFSLAVLAGVLAVLGYSVNESVVVFDRVRENFRK
                     MRRASVPEVIDAAITGVISRTIITHLCTQAMATTMFFFGGAALHYFALALTIGICFGI
                     YSSVLVASPLLVWLRVKREWFAPPLKRKGKDEHEGAVV"
     gene            complement(2372..4207)
                     /gene="secD"
                     /locus_tag="VGO02_02240"
     CDS             complement(2372..4207)
                     /gene="secD"
                     /locus_tag="VGO02_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEV7476538.1"
                     /translation="MNRYPTWGYVLIGVSILLALLYTVPNFYGESPAVQVAAAKATVK
                     VDTALLARVEETLKKGNIAYTRLAADPTGIKARFADGDTQLRAYDLLNQTLNPEKDNP
                     SYAVALNLLPASPNWLAAIKATPMTLGLDLRGGVHFLLQVDMKEAVKKRMEAFTADIR
                     QQLRDRNIRHTGVTREGDALRVRFADPAMRAKAYEAISASIPDLVLAERDDGQGGTIT
                     ATIRADALKRIQESALKQNISTLNNRVNGLGVSEPVIQQQGTDRIAVQLPGVQDPVRA
                     REILGRTATLEVRLVDEEAMRSGNHAGADVFPENRRDGTKIMVPVKRQVILTGDQFVG
                     AGATFDNDHRPAVSVDLDPAGGRVMRQVTRENLKHQMAIILFEKGKGEAISVATIQGE
                     FGSRFQITGNFSPVETNNLSLLIRSGALAAPMEIIEERTVGPSLGKENIERGFHSTLW
                     GFVAIVIFMSIYYSLFGVISSAALGLNLLFLVGLLSMLPTTLTLPGIAAIALTLGMAI
                     DANVLINERIREELRGGATPQAAIHAGYERAWATIFDSNVTTFIVGASLLLVPGAVRG
                     FAVVHCLGILTSIFSSVVVSRAMINLIYGHRRKVEKLAIGNTSWK"
BASE COUNT          662 a         1450 c         1489 g          677 t
ORIGIN      
        1 agcacgcagc cacgtgctcc tgtggctggc gctcgcgacc accggctccg tcctgctgct
       61 cgccgtcacc aaccacctga cgcagaacgt cgcctcggtg ccgctgctgt ggctcgcgcc
      121 gctcaccatc tatctgctga gcttcatcct gaccttcgag gggcagggct ggtaccggcc
      181 gcgctggatg tggcccgtgg tgctcgccgc gctcggcgtc atggtgtgga tgctggtcga
      241 cgagcgctac cactacgagc tcgcgctgca gctcggcgtg ttcctgccgg gcctgctgat
      301 cggctgcttc ttctgccatg gcgagctcta ccggctgcgc ccgggcgccg agcgcctgac
      361 cacgttctac ctcacggtcg ccgccggcgg ggcgatcggc ggcttgctgg tggccgtggc
      421 ggcgccgctc gccttcaacg gctactacga gctcggcgcg gcgctcatcg ccctcgccac
      481 gctcgccgtc gtgcgcttca gcgccatcaa cctcgccgcg cgcatcgcca gcctggcgct
      541 gctcgtcggc gcgtgggcgg gcgccgtcta cgacggcttc accgacaaga aggacgtgct
      601 cgccgcctcg cgcagcttct acggcgtgct gcgcgtaaag gagtacggcg agccgggcca
      661 ggagtcgcac ctgcggcgcc tggtgcacgg caccatcatg cacggcgagc agcacatgta
      721 cggcgagctg cgccgccggc caacgaccta ctacgaggag acctcgggag tgggcgccgc
      781 catcgagtcg cggcaggaca agccgatgcg ggtgggcgtc atcggcctgg gcaccggcac
      841 catcgccgcg tacggccgcg ccggcgactt ctacaagttc tacgagatcg accccgacgt
      901 ggtgcgcgtg gcgagcagcg acttcaccta cctttccgac agcccggcca aggtggaggt
      961 cgccctcggg gacgcgcggc tcaccctcga gcgcgaggcg ccgcagcgct tcgacgtgct
     1021 cgcgatcgac gccttctcga gcgacgccat cccggtgcac ctcatcacgc gcgaggcgct
     1081 ggcggtctac ctgaagcacg ccggcgccga cggcatcgtc gcgttccacg tctcgaaccg
     1141 cttcctcgac ctgatcccgg tggtggcgcg catcgccagg gagaacggcg cccacgcggt
     1201 gctggtgcgc gacgacccgg acgaggacga agaccaccgc tcgcgtaccg actgggtgct
     1261 ggtgtcgcgc gacccgaagg cgctcgagcg cgcggcgatc gtcaaggcca gcgccgagcc
     1321 ggctaaggac cgcccggaat ggcgcacctg gacggacgac tactcgaacc tggtacagat
     1381 tctcagataa aaatggggtc tgaccccatt taaacgattt aaacgacggc gccttcgtgc
     1441 tcgtccttgc ccttgcgctt cagcggcggg gcgaaccact cgcgcttgac gcgcagccag
     1501 accaggagcg ggctcgccac cagcaccgag gagtagatgc cgaagcagat gccgatggtg
     1561 agggcgagcg cgaagtagtg cagggcggcg ccgccgaaga agaacatggt ggtcgccatc
     1621 gcctgggtgc acaggtgggt gatgatggtg cgcgagatga ccccggtgat cgcggcgtcg
     1681 atgacttccg gcaccgaggc gcggcgcatc ttcctgaagt tctcgcgcac ccggtcgaac
     1741 accaccaccg actcgttcac cgagtagccc agcaccgcca gcacgccggc cagcactgcg
     1801 agcgagaatt cccactggaa gaaggcgaag aaccccagga tgatcaccac gtcgtgcagg
     1861 ttggcgacga tggcggcgag gccgaagcgc cagttgacga agcgcagcca caggtaggcg
     1921 acgatcgcca ccgcgaccag gagcagcgcc accgcgccca tctcggcgag ctcgccgccg
     1981 acctgcggcc cgacgaactc gacgcggcgc acttccgcgc tcttgtcgtc ggcctgcaag
     2041 agcgcggtga tcttctgcga cacctcggcg cccgggacgt ccttgatgac cggcaggcgg
     2101 atcagcacgt cgcgcgagct gccgaagttc tgcacctcgg ccttgaagcc ggccttgtcg
     2161 aggctgtcgc gcacccgctc gacctcggcg gggtgctggt agctcacctc catcagcgtg
     2221 ccgccggtga actccaccga gaagtgcagc ccgcgcgtgg cgaggaagaa caccgcggcg
     2281 aggaaggtga tcagcgagat gacgttgaag acgagcgcat gccgcatgaa cgggatggtg
     2341 ccgcggatct tgaagaactc catgcgctag ctcacttcca tgaggtgttg ccgatcgcca
     2401 gcttctccac cttgcggcgg tggccgtaga tcaggttgat catggcgcgc gacaccacca
     2461 cggaggagaa gatcgaggtc aggatgccca ggcagtgcac caccgcgaag ccgcgcaccg
     2521 ccccgggcac cagcagcagc gaggcgccga cgatgaaggt ggtgacgttc gagtcgaaga
     2581 tcgtcgccca ggcgcgctcg tagccggcgt ggatcgcggc ctgcggcgtg gcgccgccgc
     2641 gcagctcttc gcggatgcgc tcgttgatca gcacgttggc gtcgatcgcc atgcccagcg
     2701 tgagcgcgat ggcggcgatg cccggcagcg tgagggtggt cggcagcatc gacagcagcc
     2761 ccaccaggaa gagcaggttc aggcccagcg cggccgacga gatgacgccg aacagggagt
     2821 agtagatcga catgaagatc acgatggcga cgaagcccca cagcgtcgag tggaagccgc
     2881 gctcgatgtt ctccttgccc aggctcggcc cgacggtgcg ctcctcgatg atctccatcg
     2941 gcgcggcgag cgcgcccgag cggatcagca gcgacaggtt gttggtctcc accgggctga
     3001 agttgccggt gatctggaag cggctgccga actcgccctg gatggtggcc accgagatcg
     3061 cctcgccctt gcccttctcg aacaggatga tggccatctg gtgcttgagg ttctcgcgcg
     3121 tcacctggcg catgacgcgg ccgccggccg ggtcgaggtc gacgctcacc gccgggcggt
     3181 ggtcgttgtc gaacgtcgcc ccggcgccga cgaactggtc gccggtgagg atcacctggc
     3241 gcttcaccgg caccatgatc ttcgtgccgt cgcgccggtt ctcggggaag acatcggcgc
     3301 cggcgtggtt gccgctgcgc atcgcctcct cgtcgaccag gcgcacctcc agcgtggcgg
     3361 tgcggccgag gatctcgcgc gcgcgcaccg ggtcctgcac cccgggcagc tgcaccgcga
     3421 tgcggtcggt gccctgctgc tggatcaccg gctccgacac gcccagcccg ttgacgcggt
     3481 tgttgagcgt gctgatgttc tgcttgagcg ccgactcctg gatgcgcttg agcgcgtcgg
     3541 cgcggatggt ggcggtaatc gtgccgccct ggccgtcgtc gcgctcggcg agtaccaggt
     3601 cggggatgct ggcggagatc gcctcgtagg ccttggcgcg catggcgggg tcggcgaagc
     3661 gcacgcgcag cgcgtcgccc tcgcgcgtca cgccggtgtg gcggatgttg cggtcgcgca
     3721 gctgctggcg gatgtcggcg gtgaaggcct ccatgcgctt cttgaccgcc tccttcatgt
     3781 cgacctgcag gaggaagtgc acgccgccgc gcaggtccag gccgagggtc atcggcgtcg
     3841 ccttgatcgc cgccagccag ttgggcgagg cgggcagcag gttgagcgcc accgcgtagg
     3901 aggggttgtc cttctccggg ttgagcgtct ggttgagcag gtcgtaggcg cgcagctgcg
     3961 tgtcgccgtc ggcgaagcgc gccttgatcc cggtcgggtc ggcggcgagg cgggtgtagg
     4021 cgatgttgcc cttcttcagc gtctcctcga cgcgagccag gagcgccgtg tcgaccttta
     4081 ccgtggcttt ggccgccgcc acctgcacgg ccggcgactc gccgtagaag ttcggcacgg
     4141 tgtagagcag cgcgagcagg atcgaaaccc cgatcagcac gtagccccag gtgggatagc
     4201 ggttcacgcg tctactgcag cgacttgatg gtgcccttgg gcaggggcgc ctggaccgcg
     4261 gagcgctgca ccacgatc
//
LOCUS       DASYJA010000104         4506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1832886, whole genome shotgun sequence.
ACCESSION   DASYJA010000104 DASYJA010000000
VERSION     DASYJA010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832886"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VGO02_02245"
     CDS             complement(<1..352)
                     /locus_tag="VGO02_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV7476539.1"
                     /translation="MQPHGFLEQALIYLAAGVIAVPLFARLGLGSVLGYLAAGVAIGP
                     WGLALIAQPQTVLEFAEFGVVMLLFLIGLELDPERLWALRRSIFGMGALQVLATSAAV
                     TGVALLLGQSLPVAL"
     gene            368..904
                     /locus_tag="VGO02_02250"
     CDS             368..904
                     /locus_tag="VGO02_02250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEV7476540.1"
                     /translation="MEQPVSLEQFKLGMRILAGAVNIVTSAHGGRRYGMTATAVCSAT
                     AEPPTVLACVNKLATTHGALAKSKVFCVNVLRAEDWELSTTFSGAQSGEGRFKSRDWT
                     RLATGAPVLIDALVSFDCRVVKKVTHGSHTIFLGQVEQVLIGKKGKPLLYSEGQYAKL
                     ASLTLGEPLPEGLDYWGF"
     gene            1009..1335
                     /locus_tag="VGO02_02255"
     CDS             1009..1335
                     /locus_tag="VGO02_02255"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HEV7476541.1"
                     /translation="MATKPAEEAKGKAAVAQEGARIKWDDANMRSSYANVCNVTSTRE
                     EVVMLFGINQAWNRGQKEVTIQLTDRIIISPYAAKRLVMLLDGVVKEYEKRFGALNVE
                     GPAPKQ"
     gene            1343..3199
                     /locus_tag="VGO02_02260"
     CDS             1343..3199
                     /locus_tag="VGO02_02260"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEV7476542.1"
                     /translation="MRSEAAETQDSATALAGTRTELDSAWRQFGSARSPEEFCRHWLA
                     VQCHAIAEVHQAVVVLQKPGAEAFAPIAAWPEGARERSVGEIAERALREGRGLVEGRD
                     NEAYQIAYPVRLDGAVRGVVALEIAWREAAELQAAMRQLQWGAGWLEVLLRRQADPGE
                     TARLRIKLIMQLVSSFLERPTLKEAATDLAAEVASRLGCDRVVLGVWQGRALRVEAVS
                     HSVQFDPHSNLLGAIVAAMVEAIDQRETIVFPQDGEARPVVTFAHAELAQLSGAGGIL
                     SVPLLHNGAVVGALSLERAAGLPFDPPAIELAGGLASMLGPLVHLHLQQQKTLPSNVA
                     ATTRNFWSRLVGPRNAGLKFAAGAAVLLLAFLVLASGAYRISATARVEGEIQRALTAP
                     FQGYVREAPRRAGDVVKKGELLARLDDRDLRLERTRLASQAEQYGRQYREAMAKRDRA
                     SARIVSAQTDQVGAQLALIDEQLSRTDIAAPFDGVLVSGDLSQALGAPIERGQMLFEI
                     APLDAYRVVLQVDEHRVADLKAGQRGELVLSSMPGERYPMTVSKITPVSTAREGRNFF
                     RVEAQLQPDDAARLRPGMEGVAKVTVDDRLLAWIWTRELANWVRLKAWTWTP"
     gene            3210..>4506
                     /locus_tag="VGO02_02265"
     CDS             3210..>4506
                     /locus_tag="VGO02_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476543.1"
                     /translation="MAQQGLSPAWYRVAALQPRLRSHARVHRHEYRGERWYVVTDALS
                     RRAHRFGAEAYYVLGLMNGRRSLQEIWEAATARFGEEAPTQDEIIQLLGQLHTAEVLQ
                     CEVTPDVDELLRRSQLVARRSRLAKLMSPLAIKFPLFDPDRLLQRLLPWYRPLFGPFG
                     AVLWLLVVGWGALTAASHWDALTQDLGHRALAPENLLLIALVFPLIKALHEFGHACAV
                     RAWGGEVHEMGVMLLVLMPVPYVDASAASAFPEKWRRVVVGAGGMLVELVAASIALFF
                     WLEMEPGVPRAALFNVMLIAGVSTVLFNANPLLRFDGYYILADLLEIPNLRTRAQQQL
                     SSYFQRRLFGLDLPLSDTSRRERVWLTLFAVCSFFYRISVTLAIAIFVASQYFFVGVL
                     LGLWALVGGFVMPLVSLLNYLANNPRLGRNRGRAAAASAA"
BASE COUNT          658 a         1576 c         1608 g          664 t
ORIGIN      
        1 ccagcgccac gggcaagctc tgaccgagga gcagcgcgac gcccgtcact gcggcgctgg
       61 tggcgagcac ctgcagcgcg cccatgccga agatcgagcg gcgcagcgcc cacaggcgct
      121 cgggatcgag ttccaggccg atcaggaaga gcagcatcac cacgccgaac tcggcgaact
      181 cgagcaccgt ctgcggctgc gcgatcagcg cgaggcccca tggcccgatc gcgacgccgg
      241 cggcgagata gccgagcacc gagcccaggc cgaggcgcgc gaacagcggc accgcgatca
      301 cgccggcggc gaggtagatc agcgcctgct cgaggaagcc gtggggctgc attgttagaa
      361 tggtcgcatg gaacagccgg tctctctcga gcagttcaag ctcggcatgc gcatcctcgc
      421 cggcgcggtg aacatcgtca ccagcgcgca cggcggccgg cgctacggca tgaccgcgac
      481 cgcggtgtgc tcggccaccg ccgagccgcc gacggtgctc gcctgcgtca acaaattggc
      541 gaccacgcac ggcgcgctgg ccaagagcaa ggtgttctgc gtcaacgtgc tgcgcgccga
      601 ggactgggag ctgtccacca ccttcagcgg cgcgcagagc ggcgaggggc gcttcaagtc
      661 gcgcgactgg acgcgcctcg ccaccggcgc gccggtgctg atcgatgccc tggtgtcctt
      721 cgactgccgc gtggtaaaga aagtgacgca cggcagccat accatcttcc tcggccaggt
      781 cgagcaggtg ctgatcggca agaagggcaa gccgctgctc tactcggagg gccagtacgc
      841 caagctcgcc tcgctcaccc tcggcgagcc gcttcccgaa gggctcgatt actggggctt
      901 ctgaagtccc atttttacaa gctttttcaa gtctctttcg gctcctcgtg ctacccggct
      961 gtgatctaat tgcgccttcg caaacgcagc atccaaggga gaaggaacat ggcaaccaaa
     1021 cccgccgaag aggcgaaggg gaaagcggcg gtcgcgcaag aaggcgctcg catcaagtgg
     1081 gacgacgcca acatgcgcag ctcctacgcc aacgtgtgca acgtcaccag cacgcgcgag
     1141 gaggtggtga tgctgttcgg catcaaccag gcgtggaacc gcggccagaa ggaagtcacc
     1201 atccagctga ccgaccgcat catcatcagc ccgtacgcgg ccaagcgcct ggtgatgctg
     1261 ctcgacggcg tggtcaagga gtacgagaag cgcttcggcg ctctcaacgt cgaagggccg
     1321 gcgcccaagc agtgacgcac tgatgcgcag cgaggctgca gaaacgcagg acagcgccac
     1381 cgcgctggcc ggcacgcgca ccgagctgga cagcgcgtgg cgccagttcg gctcggcgcg
     1441 cagccccgag gaattctgcc ggcactggct ggcggtgcag tgccacgcca tcgccgaagt
     1501 gcaccaggcg gtggtggtgc tgcaaaagcc cggcgccgag gccttcgcgc cgatcgccgc
     1561 ctggccggag ggcgcgcgcg agcgctcggt aggcgagatc gcggagcgcg cgctgcgcga
     1621 ggggcgcggg ctggtggagg ggcgcgacaa cgaggcctac cagatcgcct atcccgtgcg
     1681 cctcgacggc gcggtgcgcg gcgtggtcgc gctcgagatc gcatggcgcg aggcggccga
     1741 gctgcaggcc gcgatgcgcc agctgcagtg gggcgccggc tggctggagg tgctgctgcg
     1801 ccgccaggcc gacccgggcg agacggcgcg cctgcgcatc aagctcatca tgcagctggt
     1861 ctcctcgttc ctcgagcgcc cgacgctcaa ggaagcggcg accgatctcg cggcggaggt
     1921 ggcctcgcgg ctgggctgcg accgcgtcgt gctcggcgtc tggcaggggc gcgcgctgcg
     1981 cgtcgaggcg gtgtcgcaca gcgtgcagtt cgacccgcat tccaacctgc tgggcgccat
     2041 cgtcgccgcc atggtcgagg ccatcgacca gcgcgagacc atcgtctttc cgcaggacgg
     2101 cgaggcgcgc ccggtggtga ctttcgcgca cgccgagctg gcgcagctct cgggcgcggg
     2161 cggcatcctg tccgtgccgc tgctgcacaa cggcgcggtg gtgggcgcgc tcagcctcga
     2221 gcgggcggcc ggcctgccct tcgacccgcc ggcgatcgag ctggccggcg gcctcgcctc
     2281 gatgctcggc ccgctggtgc acctgcacct gcagcagcag aagacgctgc cttcgaacgt
     2341 cgccgcgacg acgcgcaatt tctggtcacg cctggtcggc ccgcgcaacg ccggcctgaa
     2401 attcgccgcc ggcgccgcgg tgctgctgct ggctttcctg gtgctcgcct cgggcgccta
     2461 ccgcatctcg gccaccgcac gggtcgaggg cgagatccag cgcgcgctga ccgcgccttt
     2521 ccagggctac gtgcgcgagg cgccgcgccg cgcgggcgac gtggtgaaga agggcgagct
     2581 gctggcgcgc ctggacgatc gcgacctgcg cctggagcgc acccggctcg cctcgcaggc
     2641 cgagcagtac ggccgccagt accgcgaggc gatggccaag cgcgaccggg cgagcgcgcg
     2701 catcgtcagc gcgcagaccg accaggtggg cgcgcagctc gcgctgatcg atgagcagct
     2761 gtcgcgcacc gacatcgccg cgccgttcga cggcgtgctg gtgagcggcg acctgtcgca
     2821 ggcgctgggc gcgccgatcg agcgcggcca gatgctgttc gagattgcgc cgctcgacgc
     2881 ctaccgcgtg gtgctgcagg tcgacgagca ccgcgtcgcc gacctgaagg ccgggcagcg
     2941 cggcgagctg gtgctgtcct cgatgcccgg cgagcgctac ccgatgaccg tgtccaagat
     3001 cacgccggtg agcaccgcgc gcgaagggcg caacttcttc cgcgtcgagg cgcagctgca
     3061 gcccgacgac gcggcgcggc tgcgccccgg catggagggc gtggcgaagg tgacggtcga
     3121 cgatcgcctg ctggcctgga tctggacccg cgagctggcc aactgggtgc ggctcaaggc
     3181 ctggacgtgg acaccctgaa ggagcggcga tggctcagca ggggctgagc ccggcgtggt
     3241 atcgcgtcgc cgcgctgcag ccgcggctgc gctcgcacgc gcgcgtgcat cgccacgaat
     3301 accgcggcga gcgctggtac gtggtgaccg acgccctgtc gcgccgcgcg caccgcttcg
     3361 gcgccgaggc gtactacgtg ctcgggctga tgaacgggcg gcgcagcctg caggagatct
     3421 gggaagcggc gacggcgcgc ttcggcgagg aagcgccgac gcaggacgag atcatccagc
     3481 tgctcggcca gctgcatacc gccgaggtgc tgcagtgcga ggtgaccccc gacgtcgacg
     3541 agctgctgcg ccgctcccag ctcgtcgccc ggcgctcgcg cctcgccaag ctgatgtcgc
     3601 ccctcgccat caagttcccg ctgttcgacc ccgaccgcct cctgcagcgg ctgctgccct
     3661 ggtaccggcc gctgttcggc ccgttcggcg cggtgctctg gctgctcgtc gtcggctggg
     3721 gggcgctgac ggcggccagc cactgggatg cgctcaccca ggacctcggc catcgcgcgc
     3781 tcgcgccgga gaacctgctg ctgatcgccc tggtgttccc gctcatcaag gcgctgcacg
     3841 agttcggcca cgcctgcgcg gtgcgcgcct ggggcggcga ggtgcacgag atgggggtca
     3901 tgctgctggt gctgatgccg gtgccgtacg tcgacgcctc ggccgccagc gccttccccg
     3961 agaagtggcg ccgcgtggtg gtcggcgccg gcggcatgct ggtggagctc gtcgccgcat
     4021 cgatcgcgct gttcttctgg ctggagatgg agccgggcgt gccgcgcgcg gcgctgttca
     4081 acgtgatgct gatcgccggc gtgtcgacgg tgctgttcaa cgccaacccg ctgctgcgct
     4141 tcgacggcta ctacatcctc gccgacctgc tggaaatccc caacctgcgc acgcgcgcgc
     4201 agcagcagct ctcgtcgtat ttccagcggc gcctgttcgg cctggacctg ccgctgtcgg
     4261 atacctcgcg ccgggagcgc gtgtggctga ccctgttcgc cgtctgctcg tttttctacc
     4321 gcatctcggt caccctggcg atcgccatct ttgtcgccag ccagtacttc ttcgtcggcg
     4381 tgctgctcgg cctgtgggcg ctggtcggcg ggttcgtgat gccgctggtc agcctgctca
     4441 actatctcgc caacaacccg cgcctgggca ggaaccgcgg gcgcgccgcc gccgcgagcg
     4501 cggcgc
//
LOCUS       DASYJA010000105         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1016223, whole genome shotgun sequence.
ACCESSION   DASYJA010000105 DASYJA010000000
VERSION     DASYJA010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016223"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VGO02_02270"
     CDS             complement(<1..294)
                     /locus_tag="VGO02_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7476544.1"
                     /translation="MPALNAEVRFREVWAWSMYDFANSGYTTVVITAVFSAYFVSVVS
                     NNAPWATFAWTAALSVSYALILLTGPLLGAWADAHAAKKKLLFLSTVGCVGFTA"
     gene            complement(295..1410)
                     /locus_tag="VGO02_02275"
     CDS             complement(295..1410)
                     /locus_tag="VGO02_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7476545.1"
                     /translation="MDTPLIRDAIRQIQDLVAEATGQMLQGDAERRLSAVLEGFRSGP
                     DGKGEAAERNFSDREVTILIADLRGFAAISATYPASTVLAVLNRCFSRMSEIIVRHYG
                     SIDKFMGDAIMVVFSGDLSEPRSHVRRALLCALEMQIAMNELRTRHRSESVPELYMGI
                     GINTGWVVAGLIGSEVYRAYTVIGEEVNLTSRIEAFSLRGQVLISDSTYGHAADFAQV
                     GEPLEVYVKGKPDKVRIREVIGIPSLGKEVPRQEIRRSPRVEVRLPFRYQRLRGKEVI
                     AEVQRGVIRDIGYHGLLAEFEQPLELYAELKLSVELLQVSHEAKDVYARVVKIVPQQG
                     AHRAAHLAGLEFTSLSAVDDGKIQMFVQMALQGEERS"
     gene            1463..>2432
                     /locus_tag="VGO02_02280"
     CDS             1463..>2432
                     /locus_tag="VGO02_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEV7476546.1"
                     /translation="MQEVVKPVAQSPQRLALPEVLDWLVEDKLVGAEPAAQLKKERRY
                     YRGSLHPLAIVAEQKWKNGAAALTLDYLSEWLAKRFGLPYFHIDPLKIDFTAVTEVMS
                     AAYATRFRILPVGVTAKEAVIAISEPNVREWEPEIERMSKRSIKRVISNPADIERYQV
                     EFYNLAKSMKGAKASHAGAGGLSNFEQLVELGKGDKQFDANDAPIVRIVDWLWQYAFD
                     QRASDIHVEPRREQGIVRFRIDGVLHQVYQIPPTVLNAMTSRIKILARMDVVEKRRPQ
                     DGRIKTRTLDGDEVELRLSTLPTAFGVKLVMRIFDPEVLVRDFKELG"
BASE COUNT          430 a          811 c          798 g          393 t
ORIGIN      
        1 cgcggtgaac ccgacgcagc cgacggtcga gaggaacagc agcttcttct ttgcggcatg
       61 ggcgtcggcc caggcgccga gcagcggccc ggtgaggagg atcagcgcat acgagaccga
      121 aagggccgcc gtccaggcga acgtggccca gggggcatta ttagaaacaa cacttacaaa
      181 ataggcgctg aacaccgccg tgatcactac cgtggtgtag cccgagttgg cgaagtcgta
      241 catgctccac gcccagacct cgcggaaccg gacctctgcg tttagcgccg gcacctagct
      301 gcgctcttcg ccctgcagcg ccatctgcac gaacatctgg atcttgccgt cgtcgacggc
      361 gctgagcgag gtgaactcga gtccggcaag atgcgctgct cggtgcgcgc cttgctgcgg
      421 cacgatcttc accacccgcg cgtagacgtc ctttgcttcg tgcgacacct gcagcagctc
      481 gaccgagagc ttgagctcgg cgtacagctc gaggggctgc tcgaactccg ccagcaggcc
      541 gtggtagccg atgtcgcgga tcacgccgcg ctgtacctcg gcgatgactt ccttgccgcg
      601 cagccgctgg tagcgaaagg gcagcctcac ctcgacgcgc gggctgcgcc ggatctcctg
      661 gcgcggcacc tccttgccga gcgacgggat gccgatcacc tcgcggatgc gcaccttgtc
      721 cggcttgccc ttcacgtaca cctcgagcgg ctcgccgacc tgggcgaagt cggcggcatg
      781 gccgtaggtc gagtcgctga tcagcacctg gccgcgcagg ctgaaggcct cgatgcgcga
      841 ggtgaggttc acctcctcgc cgatcacggt gtaggcgcgg tacacctcgg agccgatcag
      901 cccggccacc acccagccgg tgttgatgcc gatgcccatg tagagctccg gcacgctctc
      961 gctgcggtgc cgggtgcgca gctcgttcat cgcgatctgc atctcgagcg cgcacagcag
     1021 cgcgcggcgc acatggctgc gcggctcgga caggtcgccg gagaacacca ccatgatggc
     1081 gtcgcccatg aacttgtcga tgctgccgta gtggcgcacg atgatctcgc tcatgcgcga
     1141 gaagcagcgg ttgagcaccg cgagcaccgt gctcgccggg taggtggccg agatggcggc
     1201 gaaaccgcgc aggtcggcga tcaggatggt gacctcgcgg tcggagaagt tgcgttccgc
     1261 cgcttcgcct ttgccgtccg ggccggagcg aaaaccctcg agaacggcgg acaggcgccg
     1321 ctcggcgtcg ccttgcaaca tttgaccggt cgcctccgcc accaggtcct gaatttggcg
     1381 gattgcgtca cggatcagcg gcgtatccat gccgcattct aggctttccc ggtatagtct
     1441 tgattcctat gcaagaagcg gaatgcaaga agtagtcaag ccggtcgctc agagcccgca
     1501 gcgtctcgcc ctgcccgagg tgctcgactg gctggtcgag gacaagctgg tcggcgccga
     1561 gccggcggcg cagctcaaga aggagcgccg ctactaccgc ggctcgctgc acccgctcgc
     1621 catcgtcgcc gagcagaagt ggaagaacgg cgccgccgcg ctgaccctcg actacctctc
     1681 ggaatggctc gccaagcgct tcggcttgcc ctatttccac atcgacccgc tgaagatcga
     1741 cttcaccgcg gtgaccgagg tgatgtccgc ggcgtacgcc acgcgctttc gcatcctgcc
     1801 ggtgggggtg acggcgaagg aagccgtgat cgccatctcc gagccgaacg tgcgcgagtg
     1861 ggagcccgag atcgagcgca tgagcaagcg cagcatcaag cgcgtgatct ccaacccggc
     1921 cgacatcgag cgctaccagg tcgagttcta caacctcgcc aagtcgatga agggggcgaa
     1981 ggcgagccac gccggcgccg gcggcctgtc caacttcgag cagctggtcg agctcggcaa
     2041 gggcgacaag cagttcgacg ccaacgacgc gcccatcgtg cgcatcgtcg actggctgtg
     2101 gcagtacgcc ttcgaccagc gcgcgagcga catccacgtc gagccgcggc gcgagcaggg
     2161 catcgtgcgc tttcgcatcg acggcgtgct gcaccaagtc taccagatcc cgccgacggt
     2221 gctgaacgcc atgaccagcc gcatcaagat cctggcgcgc atggacgtgg tggagaagcg
     2281 ccggccgcag gacggccgca tcaagacgcg cacgctcgac ggcgatgaag tcgagctgcg
     2341 cctgtcgacc ctgccgaccg cattcggcgt gaagctggtg atgcggatct tcgacccgga
     2401 agtgctggtg cgcgacttca aggagctcgg ct
//
LOCUS       DASYJA010000106         1661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_561550, whole genome shotgun sequence.
ACCESSION   DASYJA010000106 DASYJA010000000
VERSION     DASYJA010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561550"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VGO02_02285"
     CDS             <1..189
                     /locus_tag="VGO02_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476547.1"
                     /translation="HARTAYPQPGVIAGEVVERRRNPDGREVVRINTEQPYGVSSVDG
                     TAQFDVFAESLEEIGGQT"
     gene            complement(295..369)
                     /locus_tag="VGO02_02290"
     tRNA            complement(295..369)
                     /locus_tag="VGO02_02290"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(335..337),aa:Val,seq:cac)
     gene            complement(459..>1661)
                     /locus_tag="VGO02_02295"
     CDS             complement(459..>1661)
                     /locus_tag="VGO02_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476548.1"
                     /translation="GVIPLVHARAVRLTRKRIEAMTPMSLAEIQAEKDQLRAEFAMTT
                     RRLEMSVEQMKTKSNNQLAELGKKSEAIGRLRLELNEKTETMFALEARERQLIEEVAS
                     ARAELATRTEALAATEQWLAGARTQMGEVTGRLDDSSRAADSQRVEVVALRAQVEALH
                     GQIEAYEMEAKDLIARLAAKSTEAEASSRVLAEERTRTEVLGNRVNEIERHLLAQTTE
                     AEILERRVQELNMRSDEQGRLLGERDYAASQLRSETEAAARNEADMRAEFTEAENINK
                     AATERLRAEKIMLESQLKRGEEERAKLLRDIDGIRREAETASATERMENAMMRDRIAE
                     LAAEMARITSLIEGPGNPIETILAESARAPSGNGAGNGASGGDNGSLTDRIRALQTRP
                     RVAASSQV"
BASE COUNT          219 a          549 c          558 g          335 t
ORIGIN      
        1 cacgcacgga ccgcttaccc gcagccaggt gtgatcgcag gcgaggtggt cgagcgtcgc
       61 cgtaatcctg atgggcggga ggtggtccgg atcaacaccg agcagcccta tggcgtgagc
      121 tcggtggatg gtacggctca gtttgatgtg ttcgccgaat ctctggagga gataggcggc
      181 caaacctagg ggcaacaaga gaagagcaga gttgccagcg tgtgatgtcc gcgttttcgt
      241 ggcgtcttga agacgtcggc agattgccgg aaaggccgac aactggggct tctatggtgg
      301 gcgctgtagg gattgaacct acgacctctc ccgtgtgaag ggaacgctct cccgctgagc
      361 taagcgcccg accagatggc acgatttaag gcaggaccgt cgccagcgca actgccttgc
      421 cgtggctaaa ccttagcagg gatttgcgcg cccgggccct acacctggct cgacgccgcc
      481 acccgcgggc gggtctgcag ggcgcggatg cggtcggtca gcgagccgtt gtcgccgccg
      541 ctggcgccgt tgcccgcacc gtttcctgag ggcgcgcggg cgctctccgc caggattgtt
      601 tcgatcggat tgcccggccc ttcgatcagg ctggtgatgc gcgccatctc ggcggcgagc
      661 tcggcgatgc gatcgcgcat catcgcgttc tccatgcgct cggtcgcgga tgccgtctcg
      721 gcctcgcgcc ttatgccatc gatgtcgcgc aggagcttgg cgcgctcttc ctcgccgcgc
      781 ttgagctggc tttcgagcat gatcttctcg gcgcgcaggc gttcggtcgc agccttgttg
      841 atgttctccg cttcggtgaa ctcagcgcgc atgtcggcct cgttcctggc cgcggcctcc
      901 gtctcggagc gcagctggga tgccgcatag tcgcgctcgc ccaggaggcg gccctgctcg
      961 tcggaccgca tgttgagctc ctgcacgcgg cgctcaagga tctcggcttc ggtggtctgc
     1021 gccagcagat ggcgctcgat ctcgttgacg cggttcccga gcacctcggt gcgagtgcgc
     1081 tcctcggcga gcacgcggct ggaggcttcg gcctcggtgg acttggcggc gagccgcgcg
     1141 atgaggtcct tggcctccat ctcataggct tcgatctggc catgcagcgc ctcgacctgg
     1201 gcgcgcagcg cgaccacctc gacccgctgg ctgtcggcgg cccgtgagct gtcgtcgagc
     1261 ctgccggtga cctcgcccat ctgcgttctc gcgccggcga gccactgctc ggtggcggcc
     1321 agcgcctcgg tgcgcgtggc aagctccgcg cgcgcgctcg ccacctcctc gatgagctgt
     1381 ctctctctgg cctcgagcgc aaacatggtc tcggtctttt cattgagctc gagccgcagc
     1441 cgcccaatcg cctcgctctt cttgccgagc tcggcaagct ggttgttgct cttggtcttc
     1501 atctgctcga cgctcatttc cagccggcgg gtcgtcatcg cgaactcggc gcgcagctgg
     1561 tccttttcgg cctggatctc ggcgagcgac atcggggtca tggcttcgat gcgcttgcgg
     1621 gtcagccgca ccgcgcgggc atgcaccagc gggatgacgc c
//
LOCUS       DASYJA010000107         1758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1651251, whole genome shotgun sequence.
ACCESSION   DASYJA010000107 DASYJA010000000
VERSION     DASYJA010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651251"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1627)
                     /locus_tag="VGO02_02300"
     CDS             complement(173..1627)
                     /locus_tag="VGO02_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEV7476549.1"
                     /translation="MTLDRQFFGALADVVFGNPFTAKRDALIVRLAPGAPKGDLISDR
                     EALARVVAPKLEPLFRDGGAALRRLSAEDRQVAEPALLYVGYHRSVPHLDALIDRQVD
                     KSEPLPVPFGDEVIGGLVRSGFDEERAARYFALYYQLRRGFYFIQRSLTGRSESMRRL
                     REGLWNNIFTHDMRGFEAALWNRMEDFSTLLLGETGTGKGQAARAIGRSAFIPYLAAE
                     RRFAASFKDTFLAINLSQFPQGLIESELFGHRKGAFSGAIDHHQGVFERCSKHGALFL
                     DEIGEVDVPVQIKLLQVLQERTFTPVGGRDQQRFSGRVIAATNRSLGALRGDGRFRDD
                     FFYRLCSDVIEVPPLRQRLAESPGELEELVRLLVERTAASQEAALVATVLEALRRDVP
                     KGYPWPGNVRELEQAVRRVLLTGRYAPELTAAKDPEEALVEKLRAGELTAAQLLAQYS
                     AMLYRRLGSYAAVAKRTGIDPRTSRKHVGGARSG"
BASE COUNT          283 a          609 c          597 g          269 t
ORIGIN      
        1 agaccccgaa agtctccttg aagcgccgct gcaggtggaa cgggctcgcg cccacggcgc
       61 cggccagctc atcgagggtg acgcgctcgc cgcggcgctc gtccaggtag ctcttggctt
      121 tgtcgaggat cgattccatg gacggtattt tgaacgcaaa ccccgcgccc atctatccgc
      181 ttcttgcgcc gcccacatgc ttgcgcgagg tacgcgggtc gatgcccgtg cgcttggcta
      241 ccgcggcgta gcttcccagg cggcggtaaa gcatggcgct gtactgcgcg agcagctgcg
      301 ccgcggtgag ctcgccggcg cgcagcttct ccaccagcgc ctcctcggga tccttggctg
      361 cggtcagctc cggggcatag cggccggtga gcagcacccg ccgcaccgcc tgctcgagct
      421 cgcggacgtt gcccggccac gggtagccct tgggaacgtc gcgccgcagg gcttcgagca
      481 cggtggccac gagcgccgct tcctgcgagg cggcggtacg ctccaccagc aggcgcacca
      541 gctcctccag ctcgccgggc gattcggcga ggcgctggcg cagcggcggc acctcgatca
      601 cgtcggagca caggcggtag aagaagtcgt cgcggaagcg gccgtcgccg cgcagcgcgc
      661 cgaggctgcg gttggtggcg gcgatgacgc gcccggagaa gcgctgctgg tcgcgcccgc
      721 ctaccggcgt gaaggtgcgc tcctgcagca cctgcagcag cttgatctgc accggcacgt
      781 ccacctcgcc gatctcgtcg aggaacagcg cgccgtgctt gctgcagcgc tcgaacacgc
      841 cctggtggtg gtcgatggcg ccggagaagg cgcccttgcg gtggccgaac agctccgact
      901 cgatcaggcc ctgcgggaac tgcgacaggt tgatggcgag gaaggtgtcc ttgaagctgg
      961 cggcgaagcg ccgctcggcc gccaggtagg gaatgaacgc cgagcgtccg atggccctcg
     1021 ccgcctggcc cttgccggtg ccggtctcgc ccagcagcag cgtggagaaa tcctccatgc
     1081 ggttccacag cgccgcctcg aagccgcgca tgtcgtgcgt gaagatgttg ttccacagcc
     1141 cctcgcgcag gcggcgcatg gattcgctgc gcccggtgag cgagcgctgg atgaaataga
     1201 agccgcggcg cagctggtag tacagggcga agtagcgcgc ggcgcgctcc tcgtcgaagc
     1261 cgctgcgcac caggccgccg atcacctcgt cgccgaaggg caccggcagc ggctcgctct
     1321 tgtcgacctg gcggtcgatc agcgcatcga ggtgcggcac cgagcggtgg tagcccacat
     1381 agagcagcgc cggctcggcg acctgccggt cctcggcgct cagccggcgc agcgccgcgc
     1441 cgccgtcgcg gaacagcggc tcgagctttg gcgccaccac ccgcgccagc gcctcccggt
     1501 cgctgatcag atcgcccttc ggcgcgccgg gagccagccg cacgatcagc gcgtcgcgct
     1561 tcgcggtgaa ggggttgccg aacaccacgt cggccaaagc cccgaaaaac tgccgatcca
     1621 atgtcataca agaaatgtat gcctataccg cattaagtgc tagtcagtta gctttgcctg
     1681 gtgacctaca ttcttacgaa acaacgacat agcgcctggc acagccggtg cgtaagggga
     1741 gtcgcagcct aaccaaca
//
LOCUS       DASYJA010000108         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1651576, whole genome shotgun sequence.
ACCESSION   DASYJA010000108 DASYJA010000000
VERSION     DASYJA010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651576"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VGO02_02305"
     CDS             <1..436
                     /locus_tag="VGO02_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV7476550.1"
                     /translation="NKTRVAAERFDWVHTVASEKIARRLSEQRPAGLAPLNVLIQVNA
                     SGETSKAGVAPAAAAALASTMAPLKNIRLRGLMAIPEPGAPRARFHEVNALYERLKNE
                     FALDTLSMGMSDDMDTAIAEGATMVRIGTAIFGQRQKHQSAA"
     gene            456..1271
                     /gene="proC"
                     /locus_tag="VGO02_02310"
     CDS             456..1271
                     /gene="proC"
                     /locus_tag="VGO02_02310"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEV7476551.1"
                     /translation="MKIAFLGGGNMASALIGGLIAKGHDAARISVIEMSPAAREKLSA
                     RYPVRVTTAPDDAMRGADTLVLAVKPQDMKRALASLGSEVRGALVISVAAGITLETLS
                     RWLGGHRRIVRCMPNTPGLIGAGITGLFASSDVSSSEKQKAEGILRAVGEVVWLSEEK
                     LIDPVTAVSASGPAYVFWFIEQLAAVAVKLGIPQDDALKLAKQTVLGAARLAVSSEKD
                     PSQLRKDVTSKGGTTEAALNVFEQEELAERFMRAVEAASKRGEEMGREWGAED"
     gene            1273..1824
                     /locus_tag="VGO02_02315"
     CDS             1273..1824
                     /locus_tag="VGO02_02315"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEV7476552.1"
                     /translation="MFVQIATFLVDNLVAFFVVLLLVRFHFQWLRVGFRNPVGEFILA
                     TTSWIVLPLRRVIPGLAGLDLATLLAAWLLQALGLWLHALLVGAEPGVGLLLGAAAVE
                     LLRYSVYILIFAVFIQALLSWVNPEAPVMPVFNAVTRPFLRPLRRFIPPVGRVDLTPL
                     VLLLILWVVLILIDGAVRAILSA"
     gene            complement(1814..>2267)
                     /locus_tag="VGO02_02320"
     CDS             complement(1814..>2267)
                     /locus_tag="VGO02_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate dehydrogenase
                     kinase/phosphatase-domain containing protein"
                     /protein_id="HEV7476553.1"
                     /translation="EACRRDGDEVALRTALREYGQAIKELAGAGIFPGDMLLKNFGVT
                     RHDRVVFYDYDEIQPMEDVVFRRIPPAASYEDEIALEPYWPVGANDVFPEQFERFLVS
                     DPRSKEMFYADHRDLLDAAFWAGKQERVRAGVQEDVFPYPEEIRFPRR"
BASE COUNT          360 a          732 c          777 g          398 t
ORIGIN      
        1 caacaagacc cgcgttgcag ccgagcgctt cgactgggtg cacacggtgg cgagtgaaaa
       61 aattgcacgc cggctctccg agcagcggcc cgcgggtctg gcacccctta acgtgctgat
      121 ccaggtgaac gcctctggag agacttccaa ggccggcgtt gcgcctgcgg cggctgctgc
      181 gcttgcctcg acgatggctc ctttgaagaa catacgcttg cgcggattga tggcgatccc
      241 ggagccgggc gcgccacgtg cgcgctttca tgaagtaaat gccttgtatg aaaggctgaa
      301 gaacgagttc gcgctcgaca ccctgtcgat ggggatgagc gacgatatgg acacggcgat
      361 cgccgaaggc gccacgatgg tgcgcatcgg cacggccatc ttcggccaga gacagaaaca
      421 ccagagcgct gcatgaccga gcgcttgggc gagccatgaa aattgcgttc ctgggtggcg
      481 gcaacatggc aagcgcgctg atcggcggcc tgatcgccaa gggccacgac gccgcccgca
      541 tttcggtgat cgagatgagc ccggcggcgc gcgagaagct cagcgcgcgc tacccggtgc
      601 gcgtcacgac cgcgcccgac gacgccatga ggggcgccga cacgctcgtg ctggcggtga
      661 agccgcagga catgaagcgc gcgctcgcct cgctcggcag cgaggtgcgc ggggcgctgg
      721 tgatcagcgt cgccgccggc atcacgctcg agacgctgtc gcgctggctg ggcgggcacc
      781 gcaggatcgt gcgctgcatg ccgaacacac cgggactgat cggagcaggg atcaccggcc
      841 tgttcgcttc ttcagacgta agttcttctg aaaaacagaa agcggaaggc attctgcgcg
      901 ccgtgggcga ggtggtctgg ctgtccgagg aaaagctcat cgacccggtg acggcggtgt
      961 cggccagcgg gcccgcctac gtcttctggt tcatcgagca gcttgccgcc gtcgcggtga
     1021 agctgggtat cccgcaggac gatgctctga agctggccaa gcaaacggtg cttggcgccg
     1081 cgcgtctggc tgtttcttct gaaaaagatc cttcccagct aaggaaggac gtcacctcca
     1141 aaggtggcac gaccgaggcc gcgctgaacg tcttcgagca ggaggagctc gccgagcgct
     1201 tcatgcgcgc ggtcgaggcg gcgagcaagc gcggcgagga gatgggccgc gaatggggag
     1261 ccgaggactg acgtgttcgt tcagatagcg accttcctgg tcgacaacct cgtcgcgttc
     1321 ttcgtcgtgc tgctcctggt gcgcttccac ttccagtggc tgcgcgtcgg ctttcgcaac
     1381 ccggtgggcg agttcattct cgcgacgacc agctggatcg tgctgccgct gcggcgggtg
     1441 attcccggcc tggccggcct cgacctcgcc acgctgctcg cggcctggct gctgcaggcc
     1501 ctagggctgt ggttgcacgc gcttctggtc ggcgccgagc cgggagtggg cctgctcctc
     1561 ggcgcggccg cggtcgagct cctgcgctac agcgtctata tcctgatctt cgcggtgttc
     1621 atccaggcgc tgctctcgtg ggtgaacccc gaggcgccgg tcatgccggt attcaacgcc
     1681 gtgacgcggc cgttcctcag gccgctgcgg cgcttcatcc cgccggtcgg ccgggtggac
     1741 cttacgccgc tggtcctgct gctgatcctc tgggtggtgc tgatcctgat cgacggcgcg
     1801 gtgcgcgcga tcctcagcgc ctagggaagc ggatttcctc ggggtagggg aaaacgtcct
     1861 cctgcacccc ggcgcgcacg cgttcctgct tgccggccca gaacgcggcg tcgagcaggt
     1921 cgcggtggtc cgcgtagaac atctccttcg agcgcgggtc gctcaccagg aagcgctcga
     1981 actgctccgg gaagacgtcg ttcgcgccca ccggccagta aggctccagc gcaatctcgt
     2041 cctcgtagct cgccgcgggc gggatgcgcc ggaataccac gtcctccatc ggctggatct
     2101 cgtcgtagtc gtagaagacg acgcggtcgt ggcgcgtgac gccgaagttc ttgagcagca
     2161 tgtcgcccgg aaagatgccg gcgccggcca gctccttgat ggcctggccg tattcgcgca
     2221 gcgccgtccg cagcgcgacc tcatcgccgt cgcggcgaca agcctcg
//
LOCUS       DASYJA010000109         3718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1197909, whole genome shotgun sequence.
ACCESSION   DASYJA010000109 DASYJA010000000
VERSION     DASYJA010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197909"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..959)
                     /locus_tag="VGO02_02325"
     CDS             complement(18..959)
                     /locus_tag="VGO02_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7476554.1"
                     /translation="MRLLVFLLLFPIAAWAQPYPSKPVRIICSFPVGGIADIYGRIIA
                     AKLAESWGQPIVIDTRAGAGGVIAAEAGAKAAADGYTLVIGSVGTHAVNVSLFSKLPY
                     DAVKDFAAIVLLLEAEGLLVVHPSLPAQNVAELISLAKAKPLSFASAGMGSASHLAGE
                     LFKSMAHVDMTHVPYKGNVPAITDLLAGQTSLLFATMPTVLPHAKAGKLRALATMGNA
                     RAAVTPELPTIGESLKGFEVNNWIGLFAPAGTPPEIVRRWNTAVSNIMLSPEIQARLP
                     GEGAKFAPNTPEQFSKFVVDEIAKWAPVVRASGARVD"
     gene            complement(956..2386)
                     /gene="aspA"
                     /locus_tag="VGO02_02330"
     CDS             complement(956..2386)
                     /gene="aspA"
                     /locus_tag="VGO02_02330"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007739970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEV7476555.1"
                     /translation="MTQPVRVEHDLIGDRAVPADAYYGVHTLRALENFPITGTSISIY
                     PDLVVALACVKQAAAIANSELGLLDDKRAMAIRLACEEVREGKLLEHFVVDVIQGGAG
                     TSTNMNANEVIANRALELLGRAKGEYQHLHPLDHVNLSQSTNDVYPTAVKLALTFGIR
                     RLVLELMHLRKTFEDKAVEFNSVLKVGRTQLQDAVPMTLGQEFSAYAVMLGEDELRLA
                     EAASLIREINLGATAIGTGINAHPDYAALVTTRLSEVSGVAFVVSPNLVEATQDAGSF
                     VQLSGVLKRVAVKLSKTCNDLRLLSSGPRAGIGEIALPAVQAGSSIMPGKVNPVIPEV
                     VNQIAFEVIGNDITVTMAAEAGQLELNAFEPVIAHSLFKSLAHLAAGCRTLAERCVKG
                     ITANVERARRLLDESTALVTALNPFIGYARASEIAQEALRTNARVYDLVLAKGYMTRE
                     QLDEILKPEVLTRPHYSTVHKPKSEL"
     gene            2427..3329
                     /locus_tag="VGO02_02335"
     CDS             2427..3329
                     /locus_tag="VGO02_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEV7476556.1"
                     /translation="MDDLDFSQPAKKPAQPAKPAAPAQPATPAPVYNPAIARDFFATA
                     GKEERIPAGTKLFTENEKASRILLKRDKMYLLLEGQVGLTAKGKPVGSVKVGEIFGEM
                     AAISESPRSATAVAHTDCRVLAVDDKQLNAGLQSKPEFALMLMSIMINRLRGMISRLL
                     GTPTAAADAAKETRVFDKAMLAPLARGLGEQGTVRYDRGKVIMVQGQAGAFMYVVQEG
                     RVAISIRGTIVERVGPGGVFGEMALVDQGARTANANAETDCVLLAINRQIFLNLVKAD
                     PTFGVSLLTAIAERVRNTAASLNN"
     gene            3375..>3718
                     /locus_tag="VGO02_02340"
     CDS             3375..>3718
                     /locus_tag="VGO02_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV7476557.1"
                     /translation="MKRILFFAAALSLGFQAAAQDTLKKVKDSGSITIGHRDASIPFS
                     YYDDKQQVIGYAVELCLRIVDAVKKEVGNPNLKVNYQLVTSANRIPLMANGTIDLECG
                     STTNNLERQKQVA"
BASE COUNT          632 a         1228 c         1246 g          612 t
ORIGIN      
        1 tcatcggttt ctccctgtta gtccaccctc gcaccggatg ccctgactac cggcgcccac
       61 ttcgcgatct cgtcgaccac gaatttcgag aactgctcgg gcgtgttggg cgcgaacttc
      121 gcgccctcgc cgggcagccg cgcctggatc tccggcgaca gcatgatatt ggacaccgcg
      181 gtgttccagc gccgcacgat ctcgggcggc gtgccggccg gtgcgaacag gccgatccag
      241 ttgttcacct cgaagccttt cagcgactcg ccgatggtcg gcagctcggg cgtcaccgcg
      301 gcgcgtgcat tgcccatcgt cgcgagcgcg cgcagcttgc ccgccttggc gtgcggcagg
      361 acggtgggca tggtggcgaa aagaagcgac gtctgcccgg caagcaggtc ggtgatcgcg
      421 gggacgttgc ccttgtaagg cacgtgggtc atgtcgacgt gcgccatcga cttgaacagc
      481 tcgcccgcca ggtggctcgc gctgcccatg ccggcggagg cgaacgacag cggttttgcc
      541 ttggcaagag aaatgagctc ggcaacattc tgcgccggca gcgagggatg gaccacgagc
      601 aggccctcgg cctcgagcag cagcacgatg gcggcgaagt ccttcaccgc gtcgtagggc
      661 agtttcgaga acaggctgac attcacggcg tgagtgccga cgctacctat gaccagcgtg
      721 tagccgtcgg ctgccgcctt cgcgcccgcc tcggcagcga tcacgccgcc ggcgccggcg
      781 cgggtgtcga tcacgatcgg ctggccccag ctctccgcaa gcttcgcggc aatgatccgt
      841 ccgtagatat ccgcgatgcc gcccaccggg aaggagcaga tgatccggac aggcttgctc
      901 ggatagggct gggcccaggc tgcgatggga aacagcagca ggaacaccag cagtctcata
      961 gctcgctctt cggcttgtgc accgtcgaat agtgcggcct ggtcagcacc tcgggcttga
     1021 ggatctcatc gagctgctcg cgcgtcatgt agcccttcgc cagcaccagg tcgtagacgc
     1081 gcgcgttggt gcgcagcgcc tcctgcgcga tctcgctcgc gcgcgcgtag ccgatgaacg
     1141 ggttgagcgc ggtgaccagc gcggtcgact cgtcgagcag gcgccgggcg cgctcgacat
     1201 tcgccgttat tccttttacg cagcgctcgg cgagcgtgcg gcagccggcg gcgaggtgcg
     1261 cgaggctctt gaacaggctg tgggcgatca ccggctcgaa ggcgttgagc tcgagctgcc
     1321 cggcctccgc tgccatggtc accgtaatat cgttgccgat cacctcgaag gcgatctggt
     1381 tcaccacttc cgggatcacc gggttcacct tgcccggcat gatcgaggag ccggcctgca
     1441 ctgccggcag cgcgatctcg ccgatgccgg cgcgcggccc cgaggagagc aggcgcaggt
     1501 cgttgcaggt cttggacagc ttgaccgcga cgcgcttcag cacgccggag agctgcacga
     1561 acgagcccgc gtcctgcgtt gcctcgacca gattggggga gacgacgaag gcgacgccgg
     1621 agacttccga caggcgcgtg gtcaccagcg ccgcgtagtc gggatgggcg ttgatgccgg
     1681 tgccgatggc ggtggcgccg aggttgatct cgcggatcag gctggccgcc tcggcgaggc
     1741 gcagctcgtc ctcgccgagc atcacggcgt aggcggagaa ctcctggccg agcgtcatcg
     1801 gcacggcgtc ctgcagctgg gtgcgcccga ccttgaggac gctgttgaat tcgacggctt
     1861 tgtcttcgaa agtttttcgc aaatgcatca actcgagaac caggcgccgg atgccgaacg
     1921 tcaaagccag cttgaccgcg gtcgggtaga cgtcgttggt gctctgcgac aggttgacat
     1981 ggtcgagcgg gtggagatgc tggtactcgc ccttggcgcg gccgaggagc tcgagcgcgc
     2041 ggttggcgat cacctcgttg gcgttcatgt tggtggaagt gccggccccg ccctggatga
     2101 cgtcgaccac gaagtgctcg aggagcttgc cttcgcgcac ttcctcgcag gccaggcgga
     2161 tcgccatggc acgcttgtcg tcgagcaggc cgagctcgct gttggcgatc gccgccgcct
     2221 gcttcacgca ggccagggcc acgaccaggt ccgggtagat cgagatcgac gtgccggtga
     2281 tcgggaagtt ttccagcgcc cgcagcgtgt gaacgccgta gtaggcgtcg gccggcacgg
     2341 cgcggtcgcc gatcagatcg tgctctactc gcacgggctg tgtcatcggc ccccattctg
     2401 gcactatgat gaggttcgga ggagtgatgg acgatctgga tttcagccag ccggcgaaaa
     2461 agcccgcgca gccggccaag cccgccgcgc ccgcgcagcc ggcaaccccg gcgccggtct
     2521 ataacccggc gattgcgcgc gacttcttcg ccaccgccgg caaggaagag cggatcccgg
     2581 ccggcaccaa gctgttcacc gagaacgaga aggcgagccg catcctgctc aagcgcgaca
     2641 agatgtacct gctgctcgag ggccaggtcg gcctcaccgc aaagggcaag ccggtcggct
     2701 cggtgaaggt cggcgagatc ttcggcgaga tggcggcgat cagcgagtcg ccgcgcagcg
     2761 ccaccgcggt cgcgcacacc gactgccgcg tgctcgcggt ggacgacaag cagctcaacg
     2821 ccgggctgca gagcaagccc gagttcgccc tgatgctgat gagcatcatg atcaaccgtc
     2881 tgcgcggcat gatcagccgg ctgctcggca cgcccacggc agcggccgat gccgcgaagg
     2941 agacccgcgt gttcgacaaa gccatgctcg ccccgctcgc caggggcctc ggcgagcagg
     3001 gcacggtgcg ctacgaccgc ggcaaggtga tcatggtgca gggccaggcc ggcgccttca
     3061 tgtacgtggt gcaggagggc cgggtggcga tctccatccg cggcaccatc gtcgagcggg
     3121 tcggccccgg cggcgtattc ggcgagatgg ccctcgtcga ccagggggcg cgcaccgcca
     3181 acgccaacgc cgagaccgac tgcgtgctgc tcgccatcaa ccggcagatc ttcctcaacc
     3241 tggtgaaggc cgacccgacc ttcggcgtgt cgctgctcac cgccatcgcc gagcgcgtgc
     3301 gcaacacggc ggccagtctc aacaactagg gtagaatttt tccgcaacga catttcccaa
     3361 gggagttttc gaacatgaaa cgcattctgt ttttcgccgc cgcgctctcg cttggcttcc
     3421 aggccgcggc gcaggacacg ctgaagaagg tcaaggacag cggctcgatc accatcggac
     3481 accgcgacgc ctcgatcccc ttctcctact acgacgacaa gcagcaggtg atcggctacg
     3541 ccgtcgagct gtgcctgcgc atcgtcgacg cggtgaagaa ggaggtcggc aaccccaacc
     3601 tcaaggtgaa ctaccagctg gtcacctcgg ccaaccgcat cccgctgatg gcgaacggca
     3661 ccatcgatct cgagtgcggc tcgaccacca acaacctcga gcggcagaag caggtcgc
//
LOCUS       DASYJA010000110         3487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1380005, whole genome shotgun sequence.
ACCESSION   DASYJA010000110 DASYJA010000000
VERSION     DASYJA010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380005"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VGO02_02345"
     CDS             complement(<1..554)
                     /locus_tag="VGO02_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7476558.1"
                     /translation="MRVVITGASSGIGEALARHYAGEGNVLGLISRRPPNVQGLTGTI
                     VPYPLDVTDVAALERAAADFVLRFGAPDLVIANAGIGKGTSGEDLADMELLRRMLDVN
                     VVGLAATLAAFAPAMRGAGRGTLAGICSVAGFRGLAGNGAYSGSKAAAITWLESLRTE
                     MHGSGVAVVCICPGYIDTPLTQVNR"
     gene            complement(554..1201)
                     /locus_tag="VGO02_02350"
     CDS             complement(554..1201)
                     /locus_tag="VGO02_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEV7476559.1"
                     /translation="MGSPAKILLGLALAAVCLTAGAQSISPKDFQVLDPPHPVSTGER
                     IEVLEFFYYGCPVCYEAQPHIARWLLKAGPGVAILRVPAAFTESSESFARTFYTLGAM
                     NQIARLHWPLYDNHHFDGKELNEEKNVVAWVAANGVDRARFVELWHSDQVKAQVAAAK
                     KALDSYGIKGVPTFIVDGKYATSARLAGSVPHMMETVAYLVERAVGERKAGERKK"
     gene            complement(1217..1837)
                     /locus_tag="VGO02_02355"
     CDS             complement(1217..1837)
                     /locus_tag="VGO02_02355"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEV7476560.1"
                     /translation="MKRILSLLLAACALAPLAAAAQPQFAYSEVKPAVPVETDGKIEV
                     VEFFWYGCIHCYNLEPAVEKWEKTLPKDVQFRRVPAVFNDRWAIDASIFYTLEAMGLL
                     DRLHRPLFDAIHRERLRTDNQKEVMDWLQKQGVDQKKFTETMKSFGVQSKMRRAAQLT
                     ALYKIDGTPAMAVNGRYTVSTEQGRSQNGMFETVNYLVDTIRKGGK"
     gene            complement(1843..2448)
                     /locus_tag="VGO02_02360"
     CDS             complement(1843..2448)
                     /locus_tag="VGO02_02360"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEV7476561.1"
                     /translation="MARPRYMTATRKSSGGGFLLGIFVGLLLGLAISLGVAFYLNRTP
                     VPFTVAKPKDEKAPGKAPAIAGLPQGTPAAAAPGAADKPKFDFYKILPGAEETVSEKE
                     LRERLRAAKSQPPGQQEASKDVYFIQAGSFQNPADADNQKARLAILGFDSSVEPANLP
                     DKGTWYRVRLGPYKKVEEINKVRQQLAQNGIDASLVKMKEQ"
     gene            complement(2448..>3487)
                     /gene="argS"
                     /locus_tag="VGO02_02365"
     CDS             complement(2448..>3487)
                     /gene="argS"
                     /locus_tag="VGO02_02365"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004522947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV7476562.1"
                     /translation="PEEGYAGEYIEEIARETKDVKDLDAIRRHAVAYLRKEQDADLLA
                     FGVKFDVYYLESSLYTEGKVEKTVKALTASGKTYEKDGALWLRTTDYGDDKDRVVRKS
                     DGSYTYFVPDVAYHVTKWERGFRKVIDVQGTDHHSTVTRVRAGLQALGLGIPKDYPDY
                     VLHSLVKVVRGGEEVKISKRAGSYVTLRDLIDWVGRDAVRFFLVSRKADSEFVFDVDL
                     AREKSLENPVYYVQYAHARVCSVFAQGFTPPDAFPAERLTSEKELQLARRLEEYPDML
                     AVAAKELAPHSVAFYLRELAADFHSYYNAERILVEDEALRAARLALCAAVRQTLANGL
                     ALLGVSAPEKM"
BASE COUNT          535 a         1148 c         1159 g          645 t
ORIGIN      
        1 cggttgactt gcgtgagcgg cgtatcgatg tagccgggac agatgcacac cacggcaacc
       61 ccgctgccat gcatctcggt gcgcaggctc tccagccagg taatggccgc cgctttcgag
      121 ccgctgtagg cgccgttgcc ggcgaggccg cggaagccgg cgacgctgca gatgccggcc
      181 agggtgccgc ggccggcccc gcgcatcgcg ggcgcaaagg cggcgagggt ggccgccaga
      241 cccaccacgt tcacgtcgag catacggcgg agcagttcca tgtcagccaa gtcctcgccg
      301 ctggtgccct tgccgatgcc ggcgttggcg atcaccaggt ccggggcgcc gaagcgcaag
      361 acgaaatcgg cggcggcgcg ctcgagcgcg gcaacatcgg tgacatcgag cgggtagggc
      421 acgatggttc cggtgagccc ctgcacattg ggtgggcggc gcgagatcaa gccgaggacg
      481 tttccctcgc ctgcgtagtg tcgcgccagg gcttcgccga ttcccgagga agcgccggtg
      541 atgacgacgc gcatcacttc ttccgctcgc ctgctttgcg ctcccctacg gcccgctcga
      601 cgagatatgc caccgtttcc atcatgtgcg gcacgctccc cgccaggcgc gccgaggtcg
      661 cgtacttgcc gtcgacgatg aaagtcggca cgcccttgat gccgtagctg tcgagcgcct
      721 tcttcgccgc cgccacctgg gccttgacct ggtcggagtg ccacagctcg acgaagcgcg
      781 cgcggtcgac gccgttcgcc gccacccagg cgacgacgtt cttctcctcg ttcagctcct
      841 tgccgtcgaa atggtggttg tcgtagagcg gccagtgcag gcgcgcgatc tggttcatcg
      901 cgcccagcgt gtagaaggtg cgggcgaacg actcggagct ctcggtgaac gcggcaggca
      961 cgcgcaggat ggctacgccc ggcccggcct tcagcagcca gcgcgcgatg tgcggctgcg
     1021 cctcgtagca aacagggcag ccgtagtaga agaactccag cacctcgatg cgctcaccgg
     1081 tggacaccgg gtgcggcggg tcgagcacct ggaagtcctt gggcgagatc gactgcgcgc
     1141 cggcggtgag gcagaccgcg gcgagagcca ggccaagcag tatcttcgcc gggctcccca
     1201 tggaaggaac cttagcctac ttgccgccct tgcggatggt gtcgacgagg tagttgacgg
     1261 tctcgaacat gccgttctgc gaacggccct gctcggtgct caccgtgtag cggccgttta
     1321 ccgccatcgc cggggtgccg tcgatcttgt agagcgcggt gagctgcgcg gcgcggcgca
     1381 tcttgctctg cacgccgaag gatttcatcg tctcggtgaa tttcttctgg tcgacgccct
     1441 gcttctgcag ccagtccatc acttccttct ggttgtcggt gcgcaggcgc tcgcggtgga
     1501 tggcgtcgaa caggggccgg tgcagccggt cgagcaggcc catggcctcc agggtataga
     1561 agatcgaggc gtcgatcgcc cagcggtcgt tgaacaccgc cggcacgcgg cggaactgca
     1621 cgtccttggg cagggtcttc tcccactttt cgaccgccgg ctcgaggttg tagcagtgga
     1681 tgcagccgta ccagaagaac tcgacgactt cgatcttgcc gtcggtctcg accgggacgg
     1741 ccggcttgac ctcgctatag gcaaactggg gctgggcggc ggcggcgagc ggcgccaggg
     1801 cgcaggcggc gagcaggagc gaaagaattc ttttcatggt ccttactgtt ccttcatttt
     1861 gaccaggctg gcgtcgattc cgttctgcgc cagctgctgg cgtaccttgt tgatctcctc
     1921 gaccttcttg tacggcccga ggcgcacgcg gtaccaggtg cccttgtcgg gcaggttcgc
     1981 cggctcgacg ctcgagtcga agccgaggat cgcgaggcgc gccttctggt tgtccgcgtc
     2041 ggcggggttc tggaacgagc ccgcctggat gaaataaacg tccttcgagg cttcctgttg
     2101 accgggcggc tggctcttgg cggcgcgcag gcgctcgcgc agctccttct ccgacaccgt
     2161 ttcctcggcg ccgggcagga tcttgtagaa atcgaatttc ggtttgtccg cggcgccggg
     2221 cgccgccgcc gcgggggtgc cctgcggcag cccggcgatg gcgggcgcct tgccgggggc
     2281 cttctcgtcc ttgggcttcg ccacggtgaa aggcaccggc gtgcggttga ggtagaacgc
     2341 caccccgagc gagatcgcca gcccgagcag cagcccgacg aagatgccga gcagaaagcc
     2401 gccccccgag ctcttgcgcg tcgcggtcat gtaccggggt ctggccatca catcttctcc
     2461 ggggcgctga cgccgagtag cgcgagcccg ttggcgagcg tttggcgcac cgccgcgcac
     2521 agcgccagtc gcgccgcgcg cagcgcctcg tcctcgacga ggatgcgctc ggcattatag
     2581 tagctgtgga aatcggcggc gagttcgcgc aggtagaaag ccaccgagtg cggcgccagc
     2641 tccttcgcgg ctacagcgag catatcggga tattcttcga gtcgcctcgc cagctgtagt
     2701 tccttctcgc tggtcagtcg ctccgcgggg aaagcgtccg gaggagtgaa cccctgggca
     2761 aaaacactgc agacccgggc gtgcgcatac tgcacgtagt acaccgggtt ttccaggctc
     2821 ttctcgcggg cgaggtcgac gtcgaagacg aactccgagt cggccttgcg cgagacgagg
     2881 aagaagcgca ccgcgtcgcg gcccacccag tcgatgaggt cgcgcagcgt tacgtaggag
     2941 ccggcgcgct tggagatctt cacttcctcg ccgccgcgca ccaccttcac caggctgtgc
     3001 agcacatagt cgggatagtc cttgggaata ccgaggccga gcgcctgcag cccggcgcgc
     3061 acgcgcgtca ccgtgctgtg gtggtcggtg ccctgcacgt cgatcacctt gcggaagccg
     3121 cgctcccact tggtgacgtg ataggccacg tccggcacga aataggtata gctgccgtcc
     3181 gacttgcgca ccacgcggtc cttgtcgtcg ccgtagtccg tggtacgcag ccacagcgcg
     3241 ccgtccttct cgtaggtctt gccgcttgcc gtcagcgcct tgaccgtctt ctcgaccttg
     3301 ccctcggtgt agaggctcga ctcgaggtag tacacgtcga atttcacccc gaaggccaga
     3361 aggtcggcgt cttgttcctt tcgcagatag gccactgcgt gccggcggat ggcatccaaa
     3421 tccttcacat cctttgtttc tcgtgcaatc tcttcgatgt actcgcccgc gtagccttct
     3481 tccggcc
//
LOCUS       DASYJA010000111         2626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2015182, whole genome shotgun sequence.
ACCESSION   DASYJA010000111 DASYJA010000000
VERSION     DASYJA010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2015182"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VGO02_02370"
     CDS             complement(<1..821)
                     /locus_tag="VGO02_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7476563.1"
                     /translation="MDLNYSHDEAAFRDEVRSWLEANLPQDLRQKVADYAHLSKDDLV
                     RWHKILAKQGWVAPSWPKEWGGTGWNVVQRYIFEEELGYAGAPPLIPFGLTMCAAVLL
                     RFGSEAQKRRFLPRIYNGDDFWCQGYSEPGSGSDLASLKTKAIKDQDSYVVNGQKIWT
                     TLAHYADWIFCLVRTDPSVERRQEGISFLLIDMKTPGITVRPLILMDGAHEVNEVFFD
                     DVSVPAENLVHEEGKGWTVAKYLLGYERMNTGRIGESRRQLAVLKQFSSSLLNSNA"
     gene            complement(821..1588)
                     /locus_tag="VGO02_02375"
     CDS             complement(821..1588)
                     /locus_tag="VGO02_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7476564.1"
                     /translation="MKSDLFDLSGRVAVVTGSTRGIGKSIAEELARAGAKVAVSSRKA
                     EACLQVKEEFEKQGFEVLAQPCNISRKEELQALVEATTARWGRVDIVVANAASNPYYG
                     PLTGISDDAFDKIVTNNLKSVLWLAGMTLPGMAERGGGSFIVVGSIGGILANTVIGAY
                     GVSKAADHHLVRNLAAEWGPKNVRVNAIAPGLVKTEFARALWEDEKRAAERVSNTPLR
                     RLGEPRDIGGIAVFLASEAAAFITGQVIVADGGVTIL"
     gene            1701..2528
                     /locus_tag="VGO02_02380"
     CDS             1701..2528
                     /locus_tag="VGO02_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEV7476565.1"
                     /translation="MGAPEPTAPLSAPPATFSYEHEGDRQFATTLARGLEVLRCFTPL
                     EPLLGNKEISVRTGLPKPTVSRLTYTLTKLGYLRHNMRLGKYQLGSAVLSIGYPLLAS
                     MNVRQVARPLLKELADYCNGSVSMGVRDRLSMVYVESSRSGNGITTLPDIGTSVPIAQ
                     SVIGRAFLAACTPPEREAVLNQMKVKEPEAYRKYLASVNKALEDIRARGFCASLGELK
                     REVYAVGVPMRRTVDGEIVAFNCAVPAFMMKKGQLEDDIGPRLVAMVRNIEASLGLH"
BASE COUNT          460 a          901 c          808 g          457 t
ORIGIN      
        1 gcgttgctat tcaacaatga ggaactgaac tgcttgagca cggcgagctg ccgcctcgat
       61 tcgccgatgc gcccggtgtt catgcgctcg tagccgagga ggtacttcgc caccgtccag
      121 cccttcccct cctcgtgcac caggttctcc gccgggacgc tcacgtcgtc gaagaacacc
      181 tcgttcacct cgtgcgcgcc gtccatcagg atgagtgggc gcacggtgat cccgggcgtc
      241 ttcatgtcga tgaggagaaa cgagattccc tcctggcggc gctcgacgct cggatcggtg
      301 cgcaccaggc agaagatcca gtcggcgtag tgggccagcg tagtccagat cttctggcca
      361 ttgactacat aagaatcttg atctttgatt gctttcgtct tgagcgacgc cagatcgctc
      421 cccgagccgg gctcggaata gccctggcac cagaaatcgt cgccgttgta gatgcgcggc
      481 agaaaacgcc ttttctgagc ctcgctgccg aagcgcagca ggacggccgc gcacatggtg
      541 aggccgaagg gaataagcgg cggcgctccg gcgtagccga gctcctcctc gaagatatag
      601 cgctgcacca cgttccagcc ggtgccgccc cactccttcg gccaggacgg cgccacccag
      661 ccctgcttgg cgaggatctt gtgccagcgc accaggtcgt ccttcgacag atgcgcgtag
      721 tcggcgacct tctggcgcag gtcctgcggc agattggctt ccagccagga gcgcacttcg
      781 tcccggaagg cggcctcgtc gtgggagtag ttgaggtcca ttaaagaatg gtaactccgc
      841 catcggccac gatcacctgt ccggtgatga aagctgcggc ctcggaggcc agaaacaccg
      901 cgatgccgcc gatatcccgc ggctcgccga ggcggcgcag cggcgtgttt gaaacgcgct
      961 cggcggcgcg cttctcgtcc tcccagagcg cgcgggcgaa ctcggtcttc accagcccgg
     1021 gcgcgatcgc gttgacgcgc acgttcttcg gcccccactc cgccgccagg ttgcgcacca
     1081 agtggtggtc ggcggccttg gagacaccgt aggcaccaat caccgtgttg gcgaggatgc
     1141 cgccgatcga gccaaccacg atgaagctgc cgccgcctct ctcagccatg ccgggcaggg
     1201 tcatgccggc gagccacagc acgctcttca ggttgttggt gacgatcttg tcgaaggcgt
     1261 cgtcggagat gccggtgagc ggcccgtaat acgggttgct cgcggcgttg gcgacgacga
     1321 tgtcgacccg cccccacctt gcggtggtgg cctcgaccag ggcctgcagc tcctccttgc
     1381 gcgagatatt gcatggctgg gcaaggacct cgaaaccctg cttttcgaac tcctccttca
     1441 cctggaggca ggcttcggcc ttgcggctcg aaacggccac tttcgccccg gcacgcgcca
     1501 gctcctcggc gatcgatttg ccgatgccgc gcgtagagcc ggtcaccaca gcgacccggc
     1561 cgctcaaatc gaagagatcg gatttcacta tgcgaaatct agtatgacta agttgacaga
     1621 caagcccggc cgacagaata tcgaaacgcg aaacactccg cagcccggca caccagaact
     1681 agacaaaagg aaaagactac atgggagctc ccgaacccac ggcgccgctt tctgcgcccc
     1741 ccgccacctt cagctacgag cacgagggcg accgccagtt tgccaccacc ctcgcccgcg
     1801 gcctggaagt gctgcgctgc ttcacccccc tcgagccgct gctcggcaac aaggaaatct
     1861 cggtgcgcac cgggctgccc aagccgaccg tctcgcgcct cacctacacg ctgaccaagc
     1921 tcggctacct caggcacaac atgcgcctgg gcaagtacca gctcggctcg gcggtgctgt
     1981 cgatcggcta cccgctgctc gcctcgatga acgtgcgcca ggtggcgcgg ccgctgctga
     2041 aggagcttgc cgactactgc aacggctcgg tgtcgatggg ggtgcgcgac cgcctgagca
     2101 tggtctacgt cgaatccagc cgcagcggca acggcatcac cacgctgccc gacatcggca
     2161 cctcggtgcc gatcgcgcag tcggtgatcg gccgcgcctt cctcgccgcc tgcaccccgc
     2221 ctgagcgcga ggcggtgctc aaccagatga aggtcaagga gcccgaggcg taccgcaagt
     2281 acctcgcttc ggtgaacaag gcgctcgagg acatccgcgc gcgcggcttc tgcgcctcgc
     2341 tcggcgagct caagcgcgag gtgtacgccg tcggcgtgcc gatgcgccgc acggtcgacg
     2401 gcgagatcgt cgccttcaac tgcgcggtgc cggcgttcat gatgaagaag gggcagctcg
     2461 aggacgacat cgggccgcgc ctggtcgcca tggtccgcaa catcgaagct tcactgggcc
     2521 tgcactaagc tgctggacag cctcctctcg ttctttcgcg acctggatga cgaggcgcgc
     2581 ctgctgctgg tgcgctcgac gcgcatcgcc gtcatcctgc tcctcg
//
LOCUS       DASYJA010000112         1677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_199538, whole genome shotgun sequence.
ACCESSION   DASYJA010000112 DASYJA010000000
VERSION     DASYJA010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199538"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VGO02_02385"
     CDS             complement(<1..586)
                     /locus_tag="VGO02_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476566.1"
                     /translation="MRTISAAILAGILALAEPFAAQAAGLGRLTVLSPLGSPLNAEIE
                     IVSLQSGEEDGLSARLASADAYRLAGIEPSAALRTVRFAIVKRDGRPVLRVTSTSPVN
                     EPFMDLLVDLEWAGGRLTREYTVLLDPPEFKGPQAIAAAPQKPAAPSAQPLPPLEAKP
                     QAAAPAQDSGAPVQDSAAPVQDVAAPVQGQGAPVQ"
     gene            complement(677..>1677)
                     /gene="asd"
                     /locus_tag="VGO02_02390"
     CDS             complement(677..>1677)
                     /gene="asd"
                     /locus_tag="VGO02_02390"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEV7476567.1"
                     /translation="DIGRPVEPVKNAMDVAELKKLPVLISCQGGDYTGEIYPKLRREG
                     WKGYWIDAASALRMNDDAVIILDPVNMPLIKDALSKETKNFIGGNCTVSLMLMAIAGL
                     FEHDEIEWLTSMTYQAASGQGAAAMRELVEQMAAIGESAQPLLHDPASAILDIDRSVS
                     GSIRSSSLPKESIGHPLAGSLLPWIDKDLGNGQSREEWKAQAEGNKILGRQGNGRAPV
                     PMDGICVRVGAMRCHSQALTIKLRRPLPLDEVEGMLAEGNEWVRVVPNRKEESLAELT
                     PAAVTGKLSIPVGRLRKLPMGQDFLSAFTVGDQLLWGAAEPLRRMLRILLDSGRWH"
BASE COUNT          265 a          572 c          550 g          290 t
ORIGIN      
        1 cctgcaccgg cgcgccttgg ccttgtaccg gtgctgcgac atcctgcacg ggcgctgcgg
       61 agtcctgcac gggtgctccg gagtcctgcg cgggcgccgc ggcctgcggc ttcgcctcga
      121 gcggaggaag cggctgcgcg ctcggcgcgg ccggcttctg gggcgcggca gcgatcgcct
      181 gcggaccctt gaactcgggt ggatcgagca gcacggtgta ttcgcgcgtg aggcgcccgc
      241 cggcccactc gagatccacc aggagatcca tgaacggctc gttcaccggc gaggtcgacg
      301 tgacgcgcag caccggcctg ccgtcgcgct tgacgatcgc aaagcgaacg gtgcgcagcg
      361 cggcggaggg ctcgatgccg gccaggcggt aagcgtcagc ggaagcgagg cgcgcgctca
      421 gcccgtcttc ttcgccggat tgaagcgaga cgatctcgat ctccgcgttg agcggcgagc
      481 cgagcggcga caggacggta aggcggccga ggcccgcggc ctgcgcggca aacggctccg
      541 ccagggccaa gatgccggcc aggatcgctg cgctgattgt cctcacggtt cccccgcctg
      601 tatcggtctt ctaggtggac caactacctt aacatcatgg ccttacggcc gcaacttaaa
      661 gaagaactct gggggcttaa tgccaccggc ctgagtccag aagaattctt aacatccgcc
      721 gcagcggctc ggccgcgccc cacaggagct ggtcgcccac cgtgaaagcc gaaagaaaat
      781 cctgtcccat cggcagctta cgcaaccggc cgaccggaat ggacagcttg cccgtcaccg
      841 ccgccggggt gagctcggcg agactttctt ccttgcggtt gggcaccacc cgcacccatt
      901 cgttgccttc cgcgagcatg ccctcgacct cgtcgagcgg cagcggccgg cgcagcttga
      961 tggtgagcgc ctggctgtgg cagcgcatcg cccccacgcg cacgcaaatg ccgtccatcg
     1021 gaacgggggc gcggccgttg ccctggcgcc cgaggatctt gttgccctcg gcctgggcct
     1081 tccactcctc gcggctctgg ccgttgccga gatccttgtc gatccagggc agcaggctac
     1141 ccgcgagcgg atggccgatg ctctccttcg gcagcgagct cgagcggatc gagccgctga
     1201 ccgagcggtc gatgtcgagg atcgccgaag cgggatcgtg aagcagaggc tgagcgcttt
     1261 cgccgatggc cgccatctgc tccaccagct cgcgcatcgc cgccgctccc tgccccgagg
     1321 ccgcctggta ggtcatgctg gtcagccatt cgatctcgtc gtgctcgaac aggcccgcga
     1381 tggccatcag catgaggctg acggtgcaat tgccaccgat aaaatttttg gtttctttag
     1441 aaagagcatc cttgatcagc ggcatgttga ccgggtcgag gatgatcacc gcgtcgtcgt
     1501 tcatgcgcag cgcgctcgcg gcgtcgatcc aatagccctt ccagccttcg cgccgcagct
     1561 tcgggtagat ctcgccggtg tagtcgccgc cctggcagga aatgagcacc ggaagcttct
     1621 tcagctcggc gacgtccatc gcgttcttga ccggctcgac cggcctgccg atgtccg
//
LOCUS       DASYJA010000113         3789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2106202, whole genome shotgun sequence.
ACCESSION   DASYJA010000113 DASYJA010000000
VERSION     DASYJA010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2106202"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VGO02_02395"
     CDS             <1..230
                     /locus_tag="VGO02_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7476568.1"
                     /translation="WGYLWFWGPGGMTPETVDGVYRHLGRAIAHPEVRELMEKGGAEV
                     SGLAPPETLRAARDLNERWGAIIRTLGVKLD"
     gene            230..1375
                     /locus_tag="VGO02_02400"
                     /pseudo
     CDS             230..1375
                     /locus_tag="VGO02_02400"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
     gene            1380..2006
                     /locus_tag="VGO02_02405"
     CDS             1380..2006
                     /locus_tag="VGO02_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HEV7476569.1"
                     /translation="MLGRLMPREGKFFDLFNAHAERIVEGSRELAAMIGTFTELEAHA
                     QRIDAVERAADKITHETIALLHKTFITPFDRDQIHQLITTMDDILDLTQDVAESIALY
                     DLRRATPEARQLAEINQMCCERVKTAVGLLTNIKNPEAILKCCEEIDRLESDADRVLR
                     GAMSKLFRDEPDVKNLIKLRTIYELLETISDRCEDVANIIEGIVLENS"
     gene            2010..3023
                     /locus_tag="VGO02_02410"
     CDS             2010..3023
                     /locus_tag="VGO02_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEV7476570.1"
                     /translation="METLSISLWVVIALVIVALAFDFMNGFHDAANSIATVVSTRVLK
                     PYQAVLMAAFFNFVAVFVFHLSVAATVGKGIIDQGIIDHYVVFGALTGAIAWNLLTWY
                     YGIPSSSSHALIGGLCGAGVAKAGTWALIPAGILKTVVFIFISPFIGFLLGALLLLAI
                     SWIFRRSTPSRVDRLFRRLQLVSSSLYSLGHGGNDAQKTIGIIWMLLIAAGYTGTQDP
                     VPIWVIVACYVTIGFGTLFGGWRIVKTMGQRITKIRPVGGFCAETSGSIALFLATALG
                     IPVSTTHTITGSIVGVGTAQSVSAVRWGVAGDLLWAWILTIPCSAFVAGVAWWLGRHL
                     FGG"
     gene            complement(3020..3454)
                     /locus_tag="VGO02_02415"
     CDS             complement(3020..3454)
                     /locus_tag="VGO02_02415"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEV7476571.1"
                     /translation="MRFLFFIMCLFPAAAFAQVEIENAWSRATPPGAKVAAGYLTIRN
                     KSTAADRLVGASSPAAARVETHVNEKQGEVMRMREVKGYEVPAKGSFELKPGGAHLML
                     VDVRRPFKEGDRIPLVLRFQNAGEIKTELEVKGLGAQKGHAH"
     gene            complement(3451..>3789)
                     /locus_tag="VGO02_02420"
     CDS             complement(3451..>3789)
                     /locus_tag="VGO02_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451156.1"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEV7476572.1"
                     /translation="FFITDRLKRMINCSGFKVWPAEVEAMLYAHPAIQEACVIGAPDE
                     YRGETVKALVVLKIEFLKKIEPKEIIDWAREKMAAFKVPRVVEFVDALPKTATGKILW
                     RELQERENRK"
BASE COUNT          600 a         1308 c         1220 g          661 t
ORIGIN      
        1 tgtggggcta cctgtggttc tgggggccgg gcggcatgac gccggagacc gtggacgggg
       61 tctaccgcca cctcgggcgc gccatcgccc accccgaggt gcgcgagctg atggagaaag
      121 gcggcgccga ggtgtcgggc ctcgcgccgc ccgagaccct gcgcgcggcg cgcgatctca
      181 acgagcgctg gggcgcgatc atccgcaccc tcggcgtgaa gctggactga tggcaggccc
      241 tctcaccggt atccgcatcc tcgagttcga ggcgatcggc ccggcgccct tcgccggcat
      301 gctgctcgcc gacatgggcg ccgacgtgct ggtggtcgac cggccggcct cgaccgatct
      361 cggcctcaag cgcgagcgct ggtacgacgt catgatgcgc ggcaagcgct cggtgacgct
      421 cgacctgaag cagcccgctg cagttgaagt cgcactttca ttgattgaaa aaacgcatgc
      481 cttgatcgaa ggctttcgcc ccggcgtcat ggaacgcctg ggcgttggcc cggatgccgc
      541 gctcgcgcgc aacccccgcc tggtctacgg ccgcatgacg ggctggggcc aggacgggcc
      601 gctcgcgccg cgcgccggcc acgacatcaa ctacatcgcg ctcgccgggg tgctgcacgc
      661 gttcgggcgc aaaggcgagg cacccgtgcc gccgctcaac ctggtgggcg acttcggcgg
      721 cggcggcatg ctcctcggct tcggcgtcgc ctgcgcgctc ctcgaagcgc agcgctccgg
      781 caagggacag gtggtcgacg ccgcgatggt cgaaggcgcc tccctgctgg ccgccatgtt
      841 ttccggcttc ctgaaagccg gtcagttcag cgagacgcgc ggcgacaacg tgctggacag
      901 cggagccccc tggtacaacg tctatgagac gaaggatggc cgatacgtgt cgatcggctc
      961 gatcgagacc cggttcttcg aggaattgat cgaaagattg aagctcgaaa aggtcccgcc
     1021 ccagcacgac cgctcccgct ggccggagat gaaggcgctg ttcgaaagga ccttccgctc
     1081 gaagacgcgc gacgagtgag tcaagctctt cgagggctcc gacgcctgct tcgccccggt
     1141 gctctcctgg tccgaggcgc ggcgcgaccc gcacaactcc tcccgaaaaa gctttttcga
     1201 gctcgccgga gtggagcagc ccgcgcccgc gccgcgcttt tcccgcaccg cggcaagcgt
     1261 gcggcgtgcg ccgcccgagc ggggcgaggg cggcgcgcaa gcgctcgcgg aatggggctt
     1321 taacaaaacc gaaatagaag gcctaatagc gcgcggcctc ggaaccaagg agtgactcga
     1381 tgctcggacg cctgatgccc cgcgagggca agttcttcga cctgttcaac gcccacgccg
     1441 agcgcatcgt cgagggcagc cgcgagctgg cggcgatgat cggcaccttc accgagctcg
     1501 aggcgcacgc gcagcgcatc gacgccgtcg agcgggccgc cgacaagatc acgcacgaga
     1561 ccatcgcgct cctgcacaag accttcatca cgccgttcga ccgcgaccag atccaccagc
     1621 tgatcaccac catggacgac atcctcgacc tgacccagga tgtggccgag tcgatcgcgc
     1681 tgtacgacct gcgccgcgcc acgcccgagg caagacagct cgccgagatc aaccagatgt
     1741 gctgcgagcg ggtgaagacc gcggtcggcc tgctcaccaa catcaagaat cccgaggcga
     1801 tcctcaagtg ctgcgaggag atcgaccgcc tcgagtccga cgccgaccgc gtgctgcgcg
     1861 gcgcgatgtc gaagctgttc cgcgacgagc ccgacgtgaa gaacctgatc aagctgcgca
     1921 ccatctacga gctgctcgag accatcagcg accgctgcga ggacgtggcc aacatcatcg
     1981 aaggcatcgt cctggaaaac tcgtagcgcg tggagaccct ctcgatcagc ctgtgggtgg
     2041 tgattgcgct ggtgatcgtg gcgctcgcct tcgacttcat gaatggcttc cacgacgcgg
     2101 ccaactccat cgccacggtg gtctcgacgc gggtgctgaa gccctaccag gccgtgctga
     2161 tggcggcgtt tttcaacttc gtggcggtgt tcgtgttcca cctgagcgtc gcggccaccg
     2221 tgggcaaggg catcatcgac cagggcatca tcgaccacta cgtcgtgttc ggcgccctga
     2281 ccggcgccat cgcctggaac ctgctcacct ggtactacgg tattccttcc tcgtcctcgc
     2341 acgcgctgat cggcgggctg tgcggcgcgg gcgtcgcaaa ggccggcacc tgggccctga
     2401 tacccgccgg catcctgaag acggtggtat tcatcttcat ctcgcctttc atcggcttcc
     2461 tgctcggcgc gctgctgctg ctcgcgatct cctggatctt ccggcgctcg acgccgagcc
     2521 gcgtcgatcg cctgttccgc aggctgcagc tcgtctcctc gtcgctctac agcctcggcc
     2581 acggcggcaa cgacgcgcag aagaccatcg gcatcatctg gatgctgctg atcgccgccg
     2641 gctacaccgg cacgcaggac ccggtgccga tctgggtgat cgtcgcctgc tacgtgacga
     2701 tcggcttcgg cacgctgttc ggcggctggc gcatcgtcaa gaccatgggc cagcgcatca
     2761 ccaagatccg gccggtcggc ggcttctgcg cggagacttc gggctcgatc gcgctgtttc
     2821 tcgccacggc cctcggcatc ccggtgtcca cgacgcacac catcaccggc tcgatcgtcg
     2881 gcgtgggcac ggcgcagtcg gtgtccgcgg tgcgctgggg cgtggcgggc gacctgctgt
     2941 gggcatggat cctcaccatc ccctgctcgg ccttcgtcgc cggcgtcgcc tggtggctcg
     3001 gccggcacct cttcggcggt tagtgcgcgt gccccttctg cgcgcctagc cccttcactt
     3061 ccagctcggt cttgatctcg ccggcgttct ggaagcgcag gaccagcggg atcctgtcgc
     3121 cctccttgaa gggccgccgc acgtcgacca gcatcaggtg cgcgccgccg ggcttcagct
     3181 cgaagctgcc tttcgccggc acctcatagc ctttcacctc gcgcatgcgc atcacctcgc
     3241 cctgcttttc gttgacgtgc gtctcgacgc gcgcggcggc gggcgaggag gcgccgacca
     3301 ggcggtcggc ggccgtcgac ttgttgcgga tcgtcaggta gccggccgcc accttcgcgc
     3361 cgggcggcgt ggcgcgcgac caggcgttct cgatctcgac ttgggcgaac gcggccgccg
     3421 ggaagaggca catgatgaaa aacagaaatc tcatttgcgg ttttcccttt cctgcagctc
     3481 gcgccacagg atctttccgg tggcggtttt cggcagcgcg tcgacgaact cgaccacgcg
     3541 cggcaccttg aacgcggcca tcttctcgcg cgcccaatcg attatctcct taggttcgat
     3601 tttttttaaa aattcaattt ttaaaaccac cagcgccttg acggtctcgc cgcggtactc
     3661 gtcgggcgcg ccgatgacgc aggcctcctg gatcgccgga tgcgcgtaga gcatcgcctc
     3721 gacctcggcc ggccagacct tgaagcccga gcagttgatc atgcgcttga ggcggtcggt
     3781 gatgaagaa
//
LOCUS       DASYJA010000114         3848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_927229, whole genome shotgun sequence.
ACCESSION   DASYJA010000114 DASYJA010000000
VERSION     DASYJA010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927229"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VGO02_02425"
     CDS             complement(<1..253)
                     /locus_tag="VGO02_02425"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEV7476573.1"
                     /translation="MRELAKDPHVVAIGECGLDFNRNYSPHPDQEKWFVAQIELALSL
                     SKPLFLHSRDAHPRFSQIISSYKPEKAVAHCFTGERDELH"
     gene            complement(459..1289)
                     /locus_tag="VGO02_02430"
     CDS             complement(459..1289)
                     /locus_tag="VGO02_02430"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7476574.1"
                     /translation="MSAISKVGIVGLGKMGAPMARHLAEKGFTVAGCDPAAQARSTAK
                     ELGIPVLASPREVAQASELVIIVVGFDHEVETAVFGPGGIVEAARRGLIVAVGSTVAP
                     AYARRLAERLAAQGIVLLDAPLARGEAAAIAGKLLVFGAGDEKAFDACRPAFSAFASD
                     LFHLGAAGAGQVGKMVNNLILWACTSANDEGLRLGEALGVDPEKMRAALHLGSAQNWS
                     MDNRAELSGMPWAEKDMAIVLHEADVARLSLPLCGTVKETIKGLKIRMGLGLPRGSEP
                     "
     gene            1330..2289
                     /locus_tag="VGO02_02435"
     CDS             1330..2289
                     /locus_tag="VGO02_02435"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7476575.1"
                     /translation="MKKLSLAFAVLLCTLPLASLAQSWPQRPVKFILSLGPGSGADLG
                     ARLYADRLTRMWGQPVVVENRPGGDGVIAINAVIQAKDDHTLLWGPTANFVGHPYSLD
                     QMPYDPKELQPVARVSGTVVTLSVPAAMNVASLKDLVAAAKQKPGSLNWTTAVTMTDI
                     ILEGFFKTQGIDAAHIRYKQPAEAVNDLINDRIQLYSSAYAIVRAQVQGGKVKLLAVQ
                     ARNRVPGLDLPTVAEVGFPGLDFEGLVGIIAARSSNLPAAARDRIGADIKTVSKDPLV
                     AERLASTAQLNIPGDAAEFAQSIEEQVQQLAASAKLLGMKPKQ"
     gene            complement(2290..>3848)
                     /gene="mutS"
                     /locus_tag="VGO02_02440"
     CDS             complement(2290..>3848)
                     /gene="mutS"
                     /locus_tag="VGO02_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV7476576.1"
                     /translation="EKSASLHKVLRGFSDVERITSRVALRNARPRELAGLRDSLGLLP
                     TLQQAVPDGSALLDELRKDLAVPRQCLELLQKAVHPEPAARVIDGGVIADGYKAELDE
                     LRGLQVNAGEFLINLEAKERQRTGIPNLRVAYNSVHGYYIEITNSHAAKVPDDYRRRQ
                     TLKNAERYITPELKAFEDKALSARDRGLALEKLLYEELLSDLNIYLKELQTISRALAQ
                     VDVLCSFAGTSRKRNYIRPQFVDDIRLEIESGRHPVVEAQIENFIANDSRLSPTRRLL
                     LITGPNMGGKSTYMRQVALIALMAHVGSFVPARAARLGPLDQIFTRIGAADDLAGGRS
                     TFMVEMTESAAILHNATAKSLVLMDEVGRGTSTFDGLALAWAIAGYLIKNNASLTLFA
                     THYFELTRLALEHKDVANVHLDAVEHKDTVVFLHAVEEGPASQSYGLQVAALAGVPKA
                     VIRQASRYLQLLEESSVTRGNQDDLFSKTLKTEPEPQVDPLREELLKTNPDELTPREA
                     LELLYRLKKL"
BASE COUNT          619 a         1256 c         1274 g          699 t
ORIGIN      
        1 cgtgcagctc gtcgcgctcg ccggtgaagc agtgcgcgac tgccttttcc ggcttatagg
       61 aagaaattat ttgtgaaaag cggggatgtg catcccggga gtggagaaac aaaggctttg
      121 aaagagacaa agcaagttct atttgcgcaa cgaaccattt ctcctgatcg gggtgcggcg
      181 agtagttgcg gttgaagtcc aggccgcact cgccgatcgc gacgacgtgc gggtcctttg
      241 ccagctcgcg caacgcagga atcgtttccg ggccgcaatc gcgtgcgtgg tgggatgcac
      301 ccccgcggtg caggggagac cgagcgaaag ggccttgcgg ctctcttcga cgctcgcgcc
      361 ggtcacgatc accgatacgc cggccgcgcg cgcgcgcgcc aggacttccg gcaggtcgcc
      421 gtcgaagacc gagtgcgtga ggttggcgcc gatgtcgatc acggctcgct gccgcggggc
      481 aggccgaggc ccatgcggat tttcaggccc ttgatggtct ccttcaccgt gccgcacagg
      541 ggcaggctga ggcgcgccac gtccgcctcg tgcagcacga tggccatgtc cttctccgcc
      601 cacggcattc ccgagagctc ggcgcggttg tccatcgacc agttctgcgc gctgccgagg
      661 tgcagggcgg cacgcatctt ctcggggtcc acgccgagcg cctcgcccag gcgcagtcct
      721 tcgtcgttcg ccgaggtgca ggcccagagg atgaggttgt tcaccatctt gccgacctgc
      781 ccggcgccgg cggcgccgag gtggaacaga tcggaggcga aggcgctgaa cgcagggcgg
      841 caggcgtcga acgccttctc gtcgccggcg ccgaagacga ggagcttgcc ggcgatggcg
      901 gccgcctcgc cgcgcgcaag cggcgcatcg agcagcacga tgccctgcgc ggcgagccgc
      961 tccgccaggc gccgggcgta ggccggggcg acggtggagc cgacggcgac gatcagcccg
     1021 cgccgcgctg cctcgacgat gccgcccggc ccgaataccg cggtttcgac ctcgtggtcg
     1081 aagccgacca cgatgatgac cagctcgctt gcctgcgcca cctcgcgcgg cgaggcgagg
     1141 accgggatcc ccagctcctt cgcggtggag cgcgcctgcg ccgccggatc gcagccggcc
     1201 acagtgaaac ccttttcggc caggtgccgc gccattgggg cccccatctt gccgaggccg
     1261 acgattccga ctttgcttat tgcgctcatg gtgccggcga gtatagcttt gcgttccagg
     1321 aggatttaca tgaaaaagct gtcgcttgcc tttgctgtcc tgctgtgcac cctgccgctt
     1381 gcctcgctcg cccagagctg gccgcagcgg ccggtgaagt tcatcctgtc cctcggaccc
     1441 ggctccggtg ccgacctcgg tgcgcgcctc tacgccgacc ggctgaccag gatgtggggg
     1501 cagccggtgg tggtcgagaa ccgccccggc ggcgacggcg tcattgccat caacgcggtg
     1561 atccaggcga aggacgacca caccctgcta tggggtccga cggcgaattt cgtcggccat
     1621 ccgtacagcc tcgaccagat gccttacgac ccgaaggagc tccagccggt ggcgcgcgtg
     1681 tcgggcaccg tggttacgct gagcgtgccg gcggccatga acgtcgcgtc gctgaaagac
     1741 ctggttgctg ctgcgaagca gaaacccggg tcgctcaact ggacgaccgc ggtgacgatg
     1801 accgacatca tcctcgaggg tttcttcaag acgcagggca tcgatgcagc gcacatccgc
     1861 tacaagcagc ctgcggaagc ggtgaacgat ctgatcaacg atcgcatcca gctctatagc
     1921 tccgcctacg ccatcgtgcg ggcgcaggtg cagggaggca aggtaaagct cctcgccgtg
     1981 caggcacgca accgcgtgcc gggcctcgac ctgccgacgg tcgcggaggt cggcttcccc
     2041 ggactcgact tcgagggtct cgtcggcatc atcgctgccc gctcgagcaa cctgccggcc
     2101 gcggcgcgcg atcgcatcgg cgcagacatc aagacggttt ccaaggaccc gctggtcgcc
     2161 gagcgactcg cgtcgaccgc gcagctcaac ataccgggcg atgcggccga gttcgcgcaa
     2221 tcgatcgaag agcaggtcca gcagcttgcc gcctcggcga agctgctcgg aatgaagccg
     2281 aagcagtagc tacagcttct tcaggcggta gagcaactca agcgcttcgc gcggcgtgag
     2341 ctcgtcggga ttcgtcttta gcaactcttc ccggagggga tcgacttgcg gctccggttc
     2401 tgttttcaac gtttttgaaa acaaatcatc ctggttgccg cgggtcacgc tcgactcctc
     2461 cagcagctgc aggtagcggc tcgcttgccg gatcaccgct ttgggcacgc cggcgagcgc
     2521 ggcgacctgc aggccgtagc tctggctcgc cggtccttcc tcgaccgcgt gcaggaacac
     2581 taccgtgtcc ttgtgctcga ccgcgtcgag gtgcacgttg gcgacgtcct tgtgctcgag
     2641 ggcgaggcgc gtcaattcga agtagtgcgt cgcgaatagg gtgagggacg cgttgttctt
     2701 gatcaggtag ccggcgatcg cccatgcaag cgcaagtccg tcgaacgtcg aggtgccgcg
     2761 gccgacctcg tccatcagga ccaggctctt cgccgtcgcg ttgtgcagga tcgcggcgct
     2821 ctcggtcatc tcgaccatga aggtcgagcg gccgccggcg aggtcgtcgg cggcgccgat
     2881 gcgcgtgaag atctggtcga gcgggccgag ccgcgccgct cttgccggca cgaaagagcc
     2941 gacgtgcgcc atcagcgcga tcagcgccac ctgccgcatg taggtcgatt tgccgcccat
     3001 gttcgggccg gtgatcagca gcaggcgccg cgtcggcgaa agccggctgt cgttggcgat
     3061 gaagttctcg atctgcgcct cgactaccgg atggcgtcca ctctcgattt caaggcggat
     3121 gtcgtcgacg aattgcggtc gtatgtagtt tcgctttctt gaggttcctg caaaagagca
     3181 caagacgtcc acttgtgcga gcgcgcgcga aatcgtctgc agttccttca ggtagatgtt
     3241 caaatccgac agcagctcct cgtacagcaa cttctccagc gccaggccgc ggtcgcgcgc
     3301 cgagagcgcc ttgtcctcga acgctttgag ctccggcgtg atgtagcgct cggcgttctt
     3361 cagcgtctgc cgccgccggt agtcgtccgg caccttggcg gcatgggagt tggtgatctc
     3421 gatgtagtag ccgtgcaccg agttgtaggc tacgcgcagg ttcgggatgc cggttcgctg
     3481 ccgttctttt gcttctaaat ttattaaaaa ctctcctgcg ttcacctgca gcccgcgcag
     3541 ctcatccagc tcggccttgt agccgtcggc gatgacgccg ccgtcgatca cgcgcgccgc
     3601 gggctcaggg tggacggctt tctgcagcag ttcgagacat tgccgcggaa ccgcaaggtc
     3661 cttgcgcagc tcgtcgagca gcgcgcttcc atccgggaca gcctgctgca gcgttggcag
     3721 cagcccgagg ctgtcccgca ggccggcgag ctcgcgtggg cgcgcgttgc gcagcgcgac
     3781 gcgcgaggtg atccgctcca cgtcggagaa gccgcgcaga accttgtgca ggcttgccga
     3841 tttctcct
//
LOCUS       DASYJA010000115         2795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1743223, whole genome shotgun sequence.
ACCESSION   DASYJA010000115 DASYJA010000000
VERSION     DASYJA010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1743223"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /gene="ipk"
                     /locus_tag="VGO02_02445"
     CDS             <1..528
                     /gene="ipk"
                     /locus_tag="VGO02_02445"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="An essential enzyme in the nonmevalonate pathway of
                     isopentenyl diphosphate and dimethylallyl diphosphate
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEV7476577.1"
                     /translation="SDAATVLLVLNRLWGLNLRKEILLRIGEKLGADVPVFVLGRNAI
                     GEGIGERLTPIDLPSAWYLILTPQVSVSTKETFENALTHAGKGIKIPPFFPGVGRNDL
                     EPIVTARHPEVAAHLGWLKSRCPQARMTGSGASVFAEFAAEGEARALQSELPDTMRGL
                     VARGIEQHPLHDWAN"
     gene            559..635
                     /locus_tag="VGO02_02450"
     tRNA            559..635
                     /locus_tag="VGO02_02450"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:593..595,aa:Gln,seq:ttg)
     gene            686..1618
                     /locus_tag="VGO02_02455"
     CDS             686..1618
                     /locus_tag="VGO02_02455"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEV7476578.1"
                     /translation="MVFTGNANPRLAHEVAKHLNLSIGRAIVGKFSDGEVMIELLENV
                     RGKDVFVLQSTCAPTNDNLMEMLVMIDALKRSSAARVTAAIPYFGYARQDRRPRSARV
                     AISAKVVANMLTSVGVARVLTMDLHADQIQGFFDLPVDNIYAAPVLLGDVWKQRYENL
                     IVVSPDVGGVVRARALAKRLESDLAIIDKRRPKANVSEVMNVIGEVKDRTCVIMDDMV
                     DTAGTLVKAAQVLKEEGATKVVAYCTHAVLSGEAVKRIASSDIDELVVTDTIPLRDDA
                     RACKKIRQLSVGGLIAETILRIYTEESVSSLFIE"
     gene            1677..2459
                     /locus_tag="VGO02_02460"
     CDS             1677..2459
                     /locus_tag="VGO02_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEV7476579.1"
                     /translation="MAIEISARKREAQGTGASRRLRHMGMVPGILYGGDKEPVNLELD
                     HQALILNLRNERFHASILTLDLAGSKEQVLLRAVNMHPFKLQVQHIDFQRVSKDKKIH
                     MKVPLHFVNAEKSPGVKEQGGVVSHILNELDIVCFPDDLPEFIEVDLSGLAVGNSVHA
                     RQLSLPKGVELSLHKNEDPVVATVVVPQLITEDEEKVAEAAVISAADVPTTEQAAPAK
                     EGEAGAEGAAKPGDKAGAKPAAGADKGAAKPAADKGDKKDKK"
     gene            2463..>2795
                     /gene="pth"
                     /locus_tag="VGO02_02465"
     CDS             2463..>2795
                     /gene="pth"
                     /locus_tag="VGO02_02465"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEV7476580.1"
                     /translation="MIRLVVGLGNPGKEYERTRHNAGFWLVERFAAASGVALRKDPKY
                     QALVGRIEGGAAGSSTFLLLPQSFMNSSGRAVQMLAGFFKIKPEEILVVHDELDFPPG
                     TARIKQGGG"
BASE COUNT          547 a          863 c          934 g          451 t
ORIGIN      
        1 tccgacgccg cgaccgtcct gctggttctt aaccggttgt ggggattgaa tttaaggaag
       61 gaaatcctct tgcgcatcgg cgagaagctg ggcgcggatg tgccggtatt cgtgctgggc
      121 cgcaacgcga tcggcgaggg catcggcgag cggctcacgc caatcgatct accgagcgcc
      181 tggtatctga tactcacgcc gcaagtctcg gtttccacga aggaaacctt cgaaaacgcg
      241 ttgacacatg cggggaaagg aatcaaaata ccgccctttt ttccgggggt tggacgtaac
      301 gatctggagc cgatcgtgac ggcccgccat ccggaagtgg cggcgcacct ggggtggctg
      361 aaaagccgtt gtccgcaggc gcgcatgacg ggttccgggg caagcgtgtt cgccgagttc
      421 gcagccgaag gcgaggcgcg tgccctacag tcggagttgc cggatacgat gcgcggcttg
      481 gtcgccaggg gaatcgagca gcatccgctc cacgactggg cgaattgaag agttttcctg
      541 aagttgcacg tttacagatg gggagtcgcc aagctggtta aggcaccgga ttttgattcc
      601 ggcattcgag ggttcgaatc cttcctcccc agccaacaat atgaagcaga gtgaggcaat
      661 gaccaacgcg ccgctcgatc ggctgatggt gttcacgggc aacgccaacc cgcgccttgc
      721 gcacgaggtg gcgaagcacc tgaacctgtc gatcggacgc gccatcgtcg gcaagttctc
      781 ggacggcgag gtgatgatcg agctgctcga gaacgtgcgc ggcaaggacg tattcgtatt
      841 gcaatcgacg tgcgcgccga ccaacgacaa cctgatggaa atgctggtga tgatcgacgc
      901 cttgaagcgc tcctccgccg cgcgcgtcac cgccgccatc ccctacttcg gctacgcgcg
      961 ccaggaccgc aggccgcgct cggcgcgggt ggcgatcagc gccaaggtgg ttgccaacat
     1021 gctcacctcg gtgggcgtgg cgcgtgtgct gacgatggac ctgcacgccg accagatcca
     1081 gggcttcttc gacctgccgg tggacaacat ctatgccgcg ccggtgctgc tcggcgacgt
     1141 gtggaagcag cgctacgaga acctgatcgt ggtgtcaccc gacgtcggcg gcgtggtgcg
     1201 ggcgcgggcg ctggccaagc gcctcgaatc ggacctggcg atcatcgaca agcgccggcc
     1261 gaaggccaat gtctccgagg tcatgaacgt gatcggcgag gtaaaggacc gcacctgcgt
     1321 gatcatggac gacatggtcg acaccgccgg caccctggta aaagccgcgc aggtactgaa
     1381 ggaagagggc gccaccaagg tggtcgcgta ctgtacccac gcggtgctct ccggcgaggc
     1441 ggtgaagcgc atcgcctcct cggacatcga cgagctggtg gtgaccgaca cgattccgct
     1501 gcgcgacgac gcgcgcgcct gcaagaagat caggcagctg tcggtcggcg ggctgatcgc
     1561 cgaaacaatc ttgcgtatct acaccgaaga gtcggtttct tcgctgttta tcgagtagtt
     1621 tctcaaaacc gccggtctct ggtcgcgggg ccggtgataa cccaaggagt cacgaaatgg
     1681 ctatcgaaat cagcgcgcgc aagcgcgaag cgcaaggtac gggtgcgagc cgccgcctgc
     1741 gccacatggg catggtgccc ggcatcctct acggcggcga caaggagccg gtgaacctcg
     1801 agctcgacca ccaggcgctg atcctcaacc tgaggaacga gcgcttccac gcctcgatcc
     1861 tgacgctcga cctcgccggc tcgaaggagc aggtgctgct gcgcgcggtg aacatgcacc
     1921 cgttcaagct gcaggtgcag cacatcgact tccagcgcgt ctccaaggac aagaagatcc
     1981 acatgaaggt gccgctgcac ttcgtcaacg ccgagaagtc gccgggcgtg aaggagcaag
     2041 gcggcgtcgt cagccacatc ctgaacgagc tcgacatcgt ctgcttcccg gacgacctgc
     2101 cggagttcat cgaggtcgac ctgagcggcc tggcggtcgg caactcggtg cacgcgcgcc
     2161 agctgagcct gcccaagggc gtcgagctgt cgctgcacaa gaacgaggac ccggtggtgg
     2221 ccaccgtcgt cgtgccgcag ctcattaccg aggacgagga gaaggtcgcc gaagccgccg
     2281 tgatctcggc cgccgacgtg ccgaccaccg agcaggccgc gccggcgaag gaaggcgagg
     2341 cgggggccga gggcgccgcc aagcccggcg acaaggcggg cgccaaacct gccgctggtg
     2401 ccgacaaggg cgccgccaag cctgccgccg acaagggcga caagaaagac aagaagtaat
     2461 tcgtgatccg tctcgtagta ggcctgggca acccgggcaa ggagtacgag cgcacccggc
     2521 acaacgccgg gttctggctg gtcgagcgct tcgccgccgc gagcggcgtt gcgctgcgca
     2581 aggacccgaa gtaccaggcg ctagtcggcc ggatagaagg cggcgccgcc ggcagttcaa
     2641 ccttcttgct tctgccgcag agcttcatga actcgagcgg ccgcgcggtg cagatgctcg
     2701 ccgggttctt caagatcaag cccgaggaaa tcctggtggt gcatgacgag ctcgacttcc
     2761 ctcccgggac ggcgcgcatc aagcagggcg gcggt
//
LOCUS       DASYJA010000116         5951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1835120, whole genome shotgun sequence.
ACCESSION   DASYJA010000116 DASYJA010000000
VERSION     DASYJA010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5951
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1835120"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1171)
                     /locus_tag="VGO02_02470"
     CDS             complement(86..1171)
                     /locus_tag="VGO02_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7476581.1"
                     /translation="MAERILVVDDQRTNAEMVAGLLRNLGYEVQISLDGATALERVRA
                     ASPDLLLSDIMMPKMDGYELCRRLRADPATALLPVILITSADAHTERIKGIEAGADDF
                     LTKPVNWPELFGRVKRLLRVKALQDEIKDLNARLEERVRDQVSQLQRLGRLKRFFSRQ
                     VAEAIVAGGEDSLKPHRREITAVFLDLRGFTAFTDRADPDEVLELLRSYHAALGRTVD
                     EYGGTLEHFAGDGMMIFFNDPFPVDRPAERAVRMAIALQRDFLPIAEAWERLGHQVGL
                     GIGISQGEATLGVIGFEQRWEYAAIGNVPNLAARLCGQAGAGEIVLDAQTEQDVAHLA
                     DTESVGPLSLRGFQHPVAAFRLRSLKV"
     gene            complement(1174..2274)
                     /locus_tag="VGO02_02475"
     CDS             complement(1174..2274)
                     /locus_tag="VGO02_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEV7476582.1"
                     /translation="MARKAKAVVCRELNQPVVVETITVDSPQRGEVTIRIAACGVCHS
                     DLSATNGTIPLPPPLVLGHEAAGEVVEVGEGVASLKVGDHVVSSFIYMCGECRFCAAG
                     RPVLCLEQGKALTTLPDGSVRTRDAGGKSLGVFSGCGVMAEYATLHVDNLVKIDPAVP
                     LDRAALVGCAVTTGVGAVFNTAKVKPGSSVAVFGCGGVGLNVIQGARIAGAGRIVAID
                     TMQAKLDMARQFGATDVLLGKEDPVKALKKLTGGGPDYAFECVGNGELAATAYRSIGR
                     GGMAVVIGVAKPGDSTALRTMTLPFEEKTLTGSYFGSCVPRVDFPRMLALYMGGQLKL
                     DELITRRYRVEEAATAFADLESGKNARGVIVF"
     gene            2325..3752
                     /locus_tag="VGO02_02480"
     CDS             2325..3752
                     /locus_tag="VGO02_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7476583.1"
                     /translation="MLNRDKFFIDGRWTAPSGKETIDVHNAGSGEVMGRVPAGGAKDI
                     DAAVAAARAALEGWRDTPAGKRAEYLEKISAGLKAHADELAKLIAQEVGMPLKMAGRI
                     QAGLPIANFSNYARLVKEFSFEQRIGNSLVLREPVGVVGAITPWNYPLHQITLKVAPA
                     LAAGCTVVLKPSEVAPFNAFVLAEVAEAAGLPKGVLNVVTGTGAAAGEALVKHPGVDM
                     ISFTGSTRAGKRISELAAQTVKRVALELGGKSASLILDDADLAAAVKGTVNGCYLNSG
                     QTCTALTRMLVPETLYPEAARLAAEAAKGFVVGDPMSETTRLGPLTSQAQLERVRSYI
                     KQGIAEGAEVLAGGADAPEGVPAGGYYVKPTVFGKVSNNMTIAQEEIFGPVLSIIAYR
                     DEEDGIRIANDSPYGLAGAVWSKDEARAQRVARRIRAGQVDVNGGAFNMNAPFGGFKQ
                     SGHGREAGVYGLEEFLEYKSLQLKG"
     gene            3749..4525
                     /locus_tag="VGO02_02485"
     CDS             3749..4525
                     /locus_tag="VGO02_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7476584.1"
                     /translation="MKVQELLSLKGKTALVTGGSRGLGLQMAEALGEMGAKVAITARK
                     KDELDEAVSHLSSLGITCKSYVCDIGKREQIAPLADAVLKDLGKVDILINNAGATWGA
                     PAEDHPLEAWDKLVNVNLTGAFLLAQRIAKRSMIPHKWGRIINTASVAGITASDPRVV
                     RTVAYNATKHGLVGLTRQLAAEWGAHGITVNAICPGFFPSKMTKATLGKDLQLILDTT
                     PTGRVGNDEDLKGLVVLLASEASRHITGQAIAVDGGATII"
     gene            4522..4941
                     /locus_tag="VGO02_02490"
     CDS             4522..4941
                     /locus_tag="VGO02_02490"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV7476585.1"
                     /translation="MSDALQYTREYQKKIPFLSHLKILTEELGQGTAKCSLPVEKHLT
                     NSMGTVHGGVIMSLLDIALCTAARTLHPGSVGAITIDMSTSFLDGGSGARLLAEARVL
                     KDGRTMSFVEAEARNEDGTLVAKAIATVRVRQREKTT"
     gene            4938..5705
                     /locus_tag="VGO02_02495"
     CDS             4938..5705
                     /locus_tag="VGO02_02495"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HEV7476586.1"
                     /translation="MSSNPYAAPKAAVADAVTAPQGNFVPGGRGVAAGRGWDWIVSGW
                     ELFKKQPGIWIGLVVVALIIFVVLAIIPFIGSLAMAVLGPVFAAGVMLGCRRLSEGGE
                     LEIGHLFAGFKDKFGTLAAVGALNLAAQVVIMIVVMLITGASLFTLSGGGAGAGAAMG
                     IVLVLLIMLGLMVSVAMAVWFAFALVVLQGRGAVEALKESFSGCLKNIVPFLLYGIVL
                     MIAGFIAAIPLGLGYLVFLPVVAGSLYASYQDIYFTS"
     gene            complement(5695..>5951)
                     /locus_tag="VGO02_02500"
     CDS             complement(5695..>5951)
                     /locus_tag="VGO02_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476587.1"
                     /translation="SILARRYPKEMAGRVNTAVNVVGFVGMFAGQWAIGAVLSLWPPH
                     ASGGAAGYAAQGYPWALGMVWAVQLAGLAWLWSGRRVLTT"
BASE COUNT          993 a         2068 c         1975 g          915 t
ORIGIN      
        1 ctgggacgaa accgaagcgc agaccctggc gcgcgagatc agcaccagcc gttaggtttc
       61 aagccagctt caaggcctcc ctcggtcaaa ccttcagcga cctcaggcgg aacgctgcca
      121 ccgggtgctg aaagccgcgc aggctgagcg gccccaccga ctcggtgtcc gccaggtgcg
      181 cgacgtcctg ctcggtctgg gcgtcgagca cgatctcccc cgcccccgcc tgcccgcata
      241 gccgcgccgc gaggttcggc acgttgccga tcgcggcgta ttcccagcgc tgctcgaagc
      301 cgatcacgcc gagcgtcgct tcgccctgag agatgccgat ccccaggccc acctggtgcc
      361 ccagcctctc ccacgcctcg gcgatcggca ggaaatcgcg ctgcagcgcg atcgccatgc
      421 gcaccgcgcg ctcggcgggc cggtcgaccg ggaacgggtc gttgaagaag atcatcatgc
      481 cgtcgcccgc gaagtgctcg agcgtgccgc cgtactcgtc gaccgtgcgg ccgagcgcgg
      541 cgtggtagct gcgcagcagc tccagcactt catccgggtc ggcgcggtcg gtgaacgccg
      601 tgaagccgcg caggtcgagg aacaccgcgg tgatctcgcg ccggtgcggc ttgagcgagt
      661 cctcgccgcc agcaacgatg gcctcggcca cctggcgcga gaagaagcgc ttcaggcgcc
      721 ccaggcgctg cagctgcgac acctggtcgc gcacgcgctc ctccagcctg gcgttgaggt
      781 ccttgatctc gtcctgcagc gccttcaccc gcagcagccg cttgacccgg ccgaacagct
      841 ccggccagtt gaccggcttg gtcaggaagt cgtcggcgcc cgcctcgatg cccttgatgc
      901 gctcggtatg ggcgtcggcc gaggtgatga ggatgaccgg cagcagcgcg gtggccgggt
      961 cggcgcgcag cctgcggcac agctcgtagc cgtccatctt cggcatcatg atgtcggaga
     1021 gcagcaagtc cggggaggcc gcgcggaccc gttccagcgc ggtcgcccca tcgagcgaga
     1081 tctgcacctc gtagccgagg ttgcgcagca ggccggccac catctcggca ttggtgcgct
     1141 ggtcgtcgac gacgaggatg cgctcggcca tcgtcagaag acgattacgc cgcgggcgtt
     1201 cttccctgac tccaggtcgg caaaagccgt cgccgcctcc tcgacccggt agcggcgggt
     1261 gatcagctcg tccagcttga gctggccgcc catgtagagc gccagcatgc gcgggaagtc
     1321 gacgcgcggc acgcaggagc cgaaatagct gccggtgagc gtcttttcct cgaagggcag
     1381 cgtcatggtg cgcagcgcgg tggagtcgcc cggcttcgcc acgccgataa ccaccgccat
     1441 gccgccgcgc cctatcgagc ggtaggcggt cgcggcgagc tcgccgttgc cgacgcattc
     1501 gaaggcgtag tcggggccgc cgccggtcag cttcttcagc gccttcaccg ggtcttcctt
     1561 gccgagcagg acgtcggtgg cgccgaactg cctcgccatg tcgagcttcg cctgcatcgt
     1621 gtcgatggcg acgatgcgcc cggcgccggc gatgcgcgcg ccctggatca cgttcagccc
     1681 gacgccgccg cagccgaaca ccgccaccga cgagcccggc ttcaccttcg cggtattgaa
     1741 caccgcgccc acgcccgtgg tgacggcgca gccgacgagc gcggcgcggt cgaggggcac
     1801 ggcgggatcg atcttcacca ggttgtcgac gtgcagcgtg gcgtactccg ccatcacgcc
     1861 gcaacccgaa aacacgccaa gtgatttccc accggcgtcg cgagtgcgca cgctgccatc
     1921 aggcagcgtg gtgagcgctt tcccctgctc caggcagagc accggccggc cggcagcaca
     1981 gaagcggcat tcgccgcaca tatagatgaa agaggacacc acgtggtcgc cgaccttgag
     2041 cgaagcgacg ccttcgccca cttccaccac ctcgccggct gcctcgtggc ccagcaccaa
     2101 cggcggcggc agcgggatgg tgccgttggt cgccgacagg tcggaatggc acacgccgca
     2161 ggcggcgatc cggatggtga cttcgccgcg ctgcggcgaa tccaccgtaa tcgtctcgac
     2221 caccaccggc tggttcagct cgcggcagac gactgctttc gctttgcgcg ccatgcgcgt
     2281 cagtataatc ggcctcctcc cccgggaccc ccgcggattc caccatgctg aaccgagaca
     2341 aattcttcat cgacgggcgc tggaccgcgc cgtcgggcaa ggaaaccatc gacgtgcaca
     2401 acgccggcag cggcgaagtg atgggacgcg tgcccgccgg tggagcgaag gatatcgacg
     2461 ccgccgtcgc cgccgcgcgc gccgcgctgg agggctggcg cgatacgccg gccgggaagc
     2521 gcgccgagta cctcgagaaa atttccgccg gcctgaaggc gcacgccgac gagctggcga
     2581 agctcatcgc gcaggaagtc ggcatgccgc tcaagatggc cgggcgtatt caggccgggc
     2641 tgccgatcgc gaatttttcg aattacgccc ggctggtcaa agagttttcg tttgaacaaa
     2701 ggataggaaa ttccctggtg ttgcgcgagc cggtcggcgt ggtgggcgcc atcacgccgt
     2761 ggaactaccc gctgcaccag atcaccctca aggtcgcgcc ggcgctcgcc gccggctgca
     2821 cggtggtgct caagccttcc gaggtggcgc ccttcaacgc tttcgtgctc gccgaggtcg
     2881 ccgaggcggc cggcctgccg aaaggcgtgc tcaacgtcgt caccggcacc ggcgccgccg
     2941 cgggcgaggc gctggtgaag catccgggcg tcgacatgat ctcgttcacc ggctcgacgc
     3001 gcgccggcaa gcggatttcg gagctggcgg cccagaccgt caagcgcgtg gcgctcgagc
     3061 tgggcggcaa gagcgcctcg ctgatcctcg acgacgccga cctcgcggcg gcggtgaagg
     3121 gcacggtcaa cggctgctac ctcaactcgg gccagacctg caccgcgctc acgcgcatgc
     3181 tggtgccgga gactctttat ccggaagctg cccggctggc ggcggaagcg gcgaaaggct
     3241 tcgtggtggg agatcccatg tcggaaacca cgcggctggg accgctcact tcgcaggcgc
     3301 agctcgagcg ggtgcgcagc tatatcaagc agggcatcgc cgagggcgcc gaagtcctcg
     3361 ccggcggcgc ggacgcgccg gaaggcgtgc cggccggcgg ctactacgtg aagcccacgg
     3421 tgttcggcaa ggtgagcaac aacatgacca tcgcgcagga ggagatcttc ggcccggtcc
     3481 tgtcgatcat cgcctaccgc gacgaggagg acggcatccg catcgccaac gacagcccgt
     3541 acggcctggc cggcgcagtg tggtcgaagg acgaggcgcg cgcgcagcgc gtcgcgcggc
     3601 gcatccgcgc cgggcaggtc gacgtcaacg gcggcgcctt caacatgaac gcgcccttcg
     3661 gcggcttcaa gcagtccggc cacggccgcg aggccggcgt ctatggcctg gaggaattcc
     3721 tcgagtacaa gtcgctgcag ctcaaaggat gaaagttcaa gagctgctca gtctcaaagg
     3781 gaaaaccgct ctggtcaccg gcggctccag aggcctgggc ctgcagatgg ccgaggcgct
     3841 gggcgagatg ggcgcgaagg tcgcgatcac ggcgcgcaag aaggacgagc tcgacgaggc
     3901 tgtgtctcat ctttcttccc tgggaattac ctgcaagtcc tatgtctgcg acatcggcaa
     3961 gcgcgagcag atcgcgcccc tggccgacgc cgtgctgaag gacctcggca aagtcgacat
     4021 cctcatcaac aacgccggcg ccacctgggg cgcgccggcc gaggaccatc cgctcgaggc
     4081 ctgggacaag ctggtcaacg tcaacctgac cggcgccttt ttgctcgcgc agcggatcgc
     4141 caagcgctcg atgatcccgc acaagtgggg ccgcatcatc aacaccgcct cggtcgccgg
     4201 catcacggcg agcgacccgc gcgtggtgcg cacagtcgcc tacaacgcga ccaagcacgg
     4261 cctggtcggc ctgacgcgcc agctcgccgc cgagtggggc gcgcacggca tcacggtgaa
     4321 cgcgatctgc cccggcttct ttccctccaa gatgaccaag gccacgctcg gcaaggacct
     4381 gcagctgatc ctcgacacca ccccgaccgg gcgggtcggc aacgacgagg acctgaaggg
     4441 cctggtggtg ctgctcgcct ccgaggcctc gcgccacatc accggccagg cgatcgcggt
     4501 ggacggcggc gccaccatca tatgagcgac gcgctgcagt acacccgcga gtaccagaag
     4561 aagatcccct tcctttccca cctcaagatt cttaccgaag agctcggcca ggggaccgcc
     4621 aagtgctccc tgccggtgga aaagcacctc accaacagca tgggcacggt gcacggcggc
     4681 gtgatcatgt ccctcctcga catcgcgttg tgcaccgcgg cgcgcaccct gcatcccggc
     4741 tcggtcgggg cgatcaccat cgacatgagc acctccttcc tcgacggcgg aagcggcgcc
     4801 aggctgctgg ccgaagcgcg cgtcctcaag gatgggcgta ccatgagctt cgtcgaagcc
     4861 gaggcacgga atgaagatgg cacgctcgtc gccaaggcga tcgccactgt ccgagtcaga
     4921 caaagggaga aaacaacatg agctcgaacc cgtacgccgc ccccaaagcc gcggttgccg
     4981 acgcggtcac cgccccgcaa ggcaacttcg ttcccggagg gcgcggtgtc gctgccgggc
     5041 gcggctggga ttggatcgtg tccggctggg agctcttcaa gaagcagcca ggcatttgga
     5101 tcggcctggt ggtggtggcg ctgatcatct tcgtcgtgct cgccatcatc ccgttcatcg
     5161 gctcgctcgc gatggcggtc ctcggcccgg tattcgccgc cggcgtgatg ctcggctgcc
     5221 gcaggctcag cgagggcggc gagctcgaga tcgggcacct cttcgccggc ttcaaggaca
     5281 aattcggcac ccttgctgcc gtcggcgcgc tcaacctggc cgctcaggta gtcatcatga
     5341 tcgtggtgat gctgatcacc ggcgcgagct tgttcaccct gtccggggga ggagcgggag
     5401 ccggggcagc gatgggcatc gtattggtcc tcctgatcat gctagggctg atggtctccg
     5461 tggcgatggc ggtatggttc gctttcgccc tggtggtact gcagggccgc ggcgcagtcg
     5521 aagcgctcaa ggagagcttc tccggctgcc tgaagaacat cgtgccgttc ctgctctacg
     5581 gcatcgtcct gatgatcgcc ggattcatcg cagcgatccc gctcggtctg ggctacctcg
     5641 tgttccttcc ggtggtcgcc ggctcgctct acgcctccta ccaggacatc tacttcacgt
     5701 cgtaagcacc cgccggcccg accacagcca ggccaggccg gcgagctgca ccgcccacac
     5761 catgccgagc gcccagggat agccctgcgc ggcatagccc gccgcgccgc ccgaagcatg
     5821 cggcggccag aggctgagca cggcgccgat ggcccattgg ccggcgaaca tgccgacgaa
     5881 cccgaccacg ttgaccgcgg tgttgacccg ccccgccatc tccttcggat agcggcgcgc
     5941 gaggatcgag t
//
LOCUS       DASYJA010000117         5204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2198013, whole genome shotgun sequence.
ACCESSION   DASYJA010000117 DASYJA010000000
VERSION     DASYJA010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2198013"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..926
                     /locus_tag="VGO02_02505"
     CDS             120..926
                     /locus_tag="VGO02_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HEV7476588.1"
                     /translation="MFEALTLEVIDPAMLGGGVAAVIFFGVIVFLVLGRRLGNRVIAR
                     HGATAMPNVGSLETAVFALLGLLIAFSFSGALSRFDVRRAQAVQEANAIGTAYLRIGL
                     LPDSAQPRLRDSLRAYADSRIATYRKLPDVAAARLEWARSRELQGEIWAQAVAAARLP
                     GVRPGTEVLLFPALNDMFDITIVRVVAMQLHPPTIIYWMLGALALAAALLAGYQSAGE
                     PGYDWLHKICFAGIVAFTVYVILDIEHPRLGLVRIDSIDKVLIDVRAGMK"
     gene            926..1237
                     /locus_tag="VGO02_02510"
     CDS             926..1237
                     /locus_tag="VGO02_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV7476589.1"
                     /translation="MAEPERQALEAMAGPVLLEFGTSWCGHCKRAQPLIAEALAAHPG
                     VRHIKVADSSGKRLGRSFGVKLWPTLVFLKDGKEAARLVRPPGVKEIENALQRLAHPA
                     P"
     gene            complement(1208..2530)
                     /locus_tag="VGO02_02515"
     CDS             complement(1208..2530)
                     /locus_tag="VGO02_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEV7476590.1"
                     /translation="MTPATTSTTGAERSDLTRIARAGFAVMALGVLVMGGWLIAAPLS
                     GAVIAQGFVKVDMNRKVVQHQEGGIVKQLLVRDGERVRAGQVLLVIEDVKLDATFDLL
                     RTQHDGERAKAARLEAERAILPEVKFPAELVARKGEAKVADLLQRETTLFRARREALD
                     TQIALLRKQIQQTTVEARALSDQIAAEERALKLQKDELKVNEALLQQGFVQKTRLMQL
                     ERSVAEYEARWGEHRAELSKTQQRASELELRVLAQRNTYVQSAADELKELSTRLFDLE
                     ERLRPSKDAAERQRISSPIAGEVVGLRVFSHGGVVGPREVLMEIVPEDKTLIVEARIR
                     PEDINHVRAGSEAEIRLTAYKQRTTPLVAGKVNYVAADRMVDPQNNAPYYTAHIDVTA
                     QALADAGNLRMQAGMPAEVYIRTDSRTALDYLLAPVTAYLRRGMREPL"
     gene            complement(2527..4206)
                     /locus_tag="VGO02_02520"
     CDS             complement(2527..4206)
                     /locus_tag="VGO02_02520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEV7476591.1"
                     /translation="MRNLVGSLLRYFAYAGAFSLAINVLLLVPALYMLQVFDRVLASR
                     SEETLVMLSFGAVLALAMMSLLDVLRAQLLAACGLALDRSLGPDVLRSLLALTARTGG
                     AEHLNGLRDVATLRGFLVGAPVIALFDTPWLPLFLALIWLFHPLLGILAIGGALLMLG
                     LAWANERFTRQPLERVQAESRRSGRFIDAAVRSAESVDALGMREAVTRRWSALNETAL
                     REQREASRLGALFSGLTKFARQAIQMTMLGAGAWLVISQNLSSGVMIACTIILGRALA
                     PVEMLVGSWRSLVDVRGAWLRLTKLFESRPAAGAPATELPAPSGAVAAEGVMHMFPRM
                     ERPVLRGVAFALPAGESLGVIGPTAAGKSTLAKLLVGIWKPTSGAVRLDGVDVAAWDR
                     AQLGPHVGYVPQTVELFGGTIADNIARLGEPDSAAVVRAAQRAYAHDMILRLPKGYDT
                     ETGNAGEALSPGQRQRVALARALYGEPRLVVLDEPNANLDADGDDGLLRALQGMKQAG
                     VTVIIVAHRPSLLAGVDKLLVLKDGVMELYGPRAEIMARVTRAATQVRGVA"
     gene            4292..4444
                     /locus_tag="VGO02_02525"
     CDS             4292..4444
                     /locus_tag="VGO02_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476592.1"
                     /translation="MKPAQIEALAGTSLLVFSAFRRGPLGLLAFLAATGMIVHAATQR
                     AVEGFK"
     gene            4480..>5204
                     /locus_tag="VGO02_02530"
     CDS             4480..>5204
                     /locus_tag="VGO02_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEV7476593.1"
                     /translation="MKSLTAIVLLVAGEAFAQAYPAKAVTFIVPFAPGGGTDITARTV
                     SAKLAQIWARSGPVSTIVDNRGGAGGILGADAVAKAKPDGYTLLIANVGTQSINPSLY
                     PKLPYNPDTAFAPISLVCELPFVLMASPKFAPSSVKELVAYARANPGKVTYASSGNGG
                     SPHLTAELFQMLTGTQMTHVPYKGGGPAMIDLMAGHVDLLFASVLEGSGHIKAGRLKG
                     LAVTHAKRNAALPDVPTIAEAGV"
BASE COUNT          734 a         1882 c         1821 g          767 t
ORIGIN      
        1 gtctgtcacc gaaactgcta aatggcgaac ctggctgcgc cgggcgcgac gccctgcaag
       61 ggccgcgccc ggcatggctc cttagagcgc cgctcccggc gggaaggtaa gctgcggcca
      121 tgttcgaggc gctgacgctc gaggtgatcg accccgccat gctcggcggc ggagtcgccg
      181 ccgtcatctt cttcggcgtg atcgtgttcc tggtgctggg gcgccggctc ggcaaccggg
      241 tgatcgcgcg ccacggcgcg accgccatgc ccaacgtcgg ctcgctcgag acggcggtgt
      301 tcgcgctgct cggcctgctc atcgccttca gcttctccgg cgcgctgtcg cggttcgacg
      361 tgcggcgcgc ccaggcggtg caggaagcga acgccatcgg caccgcctac ctgcgcatcg
      421 gcctgttgcc cgactcggcg cagccacgcc tgcgcgacag cctgcgcgcc tacgccgatt
      481 cgcgcatcgc gacctacagg aagctgcccg acgtggcggc cgcccgcctc gagtgggcgc
      541 gctcgcgcga gctgcagggc gagatctggg cccaggcggt ggcggccgcc cggctaccgg
      601 gcgtgcgccc cgggacggag gtcttgttgt tccccgccct gaacgacatg ttcgacatca
      661 cgatcgtgcg ggtcgtggcc atgcagctgc acccgcccac gatcatctac tggatgctgg
      721 gggcgctcgc gctggccgcg gcgctgctcg ccggctacca gtcggccggc gagccgggct
      781 acgactggct gcacaagatc tgcttcgccg gcatcgtcgc cttcaccgtg tacgtgatcc
      841 tcgacatcga gcacccgcgg ctcggcctgg tgcgcatcga ctcgatcgac aaggtcctga
      901 tcgacgtgcg cgccggcatg aagtaatggc ggagccggaa aggcaggcgc tcgaggcgat
      961 ggccgggccg gtgctgctcg agttcggcac ctcctggtgc ggccactgca agcgcgcgca
     1021 acccctgatc gccgaggcgc tcgccgcgca tcccggcgtg cgccacatca aggtcgcgga
     1081 cagcagcggc aagcggctcg gccgctcgtt cggcgtaaag ctctggccga cgctggtttt
     1141 cctgaaggac gggaaggaag cggcgcggct ggtgcggccg ccgggcgtga aggaaatcga
     1201 aaatgcgcta cagcggctcg cgcatcccgc gccttagata ggcggtgacc ggggcgagca
     1261 ggtagtcgag cgcggtgcgg ctgtcggtgc ggatgtagac ctcggccggc atgccggcct
     1321 gcatgcgcag gttgcccgca tcggccagcg cctgcgcggt gacgtcgatg tgcgccgtgt
     1381 agtacggggc gttgttctgc gggtccacca tgcggtcggc cgcgacgtag ttcaccttgc
     1441 cggcgaccag cggcgtggtg cgctgcttgt aggcggtgag gcgtatctcg gcttccgagc
     1501 cggcgcgcac atggttgatg tcctcggggc ggatgcgcgc ctcgacgatc agcgtcttgt
     1561 cttccggcac gatctccatc agcacctcgc gcggcccgac cacgccgccg tggctgaaga
     1621 cgcgcagccc gaccacctcg cccgcgatcg gcgacgagat gcgctggcgc tcggccgcgt
     1681 ccttcgacgg ccgcaggcgc tcctccaggt cgaacaggcg ggtggagagc tccttcagct
     1741 cgtcggccgc gctctgcacg taggtgttgc gctgcgccag cacgcgcagc tcgagctcgg
     1801 aagcgcgctg ctgcgtcttg gagagctcgg cgcggtgctc gccccagcgc gcctcgtact
     1861 ccgccacgct gcgctcgagc tgcatcagcc gggtcttctg cacgaagccc tgctgcagca
     1921 gcgcctcgtt caccttgagc tcgtccttct gcagcttgag ggcgcgctcc tcggcggcga
     1981 tctggtcgct gagcgcccgc gcctccaccg tggtctgctg gatctgcttt ctcagcagcg
     2041 cgatctgcgt gtcgagcgcc tcgcgccgcg cgcggaacag cgtcgtctcg cgctgcagga
     2101 gatcggccac cttggcctcg cccttgcgcg cgaccagctc ggccgggaac ttcacctccg
     2161 gcaggatggc gcgctcggct tccagccggg cggccttggc gcgctcgccg tcgtgctgcg
     2221 tgcgcagcag gtcgaacgtc gcgtcgagct tgacgtcctc gatcaccagc aggacctggc
     2281 cggcgcgcac ccgctcgccg tcgcgcacca ggagctgctt gacgatgccg ccctcctggt
     2341 gctgcaccac cttgcggttc atgtcgacct tgacgaagcc ctgcgcgatg acggcgcccg
     2401 aaagcggcgc cgcgatcagc cagccgccca tgacgagcac gccgagcgcc atcaccgcga
     2461 acccggcacg cgcgatgcgc gtcaggtcgc tgcgctcggc gcccgttgtg ctcgttgtgg
     2521 caggggtcat gccaccccgc gcacctgggt ggcggcgcgc gtgacgcgcg ccatgatctc
     2581 ggcgcgcggc ccgtagagct ccatcacgcc gtccttcagc accagcagct tgtcgacccc
     2641 ggcgagcagc gagggccggt gcgcgacgat gatcacggtg acgccggcct gcttcatgcc
     2701 ctgcagcgcg cgcagcaggc cgtcgtcgcc gtcggcgtcg aggttggcgt tgggctcgtc
     2761 gagcaccacc aggcgcggct cgccgtagag cgcgcgcgcc agcgccacgc gctggcgctg
     2821 gcccggcgag agcgcctcgc cggcgttgcc ggtctcggtg tcgtagccct tgggcaggcg
     2881 caggatcatg tcgtgcgcgt acgcgcgctg cgcggcgcgc accaccgcgg ccgagtcggg
     2941 ctcgcccagg cgcgcgatgt tgtcggcgat ggtgccgccg aacagctcca ccgtctgcgg
     3001 cacgtagccg acgtgcggcc cgagctgcgc gcggtcccag gcggcgacgt cgacgccgtc
     3061 caggcgcacc gcgcccgagg tcggcttcca gatgcccacc agcagcttgg ccagggtgga
     3121 cttgcccgcc gcggtcgggc cgatcacgcc gagcgactcg ccggccggca gcgcgaacgc
     3181 cacgccgcgc agcaccgggc gctccatgcg cgggaacatg tgcatcacgc cctcggccgc
     3241 gaccgcgccg ctcggcgccg gcagctcggt ggccggcgcg ccggccgccg gccgcgactc
     3301 gaagagcttc gtcaggcgca gccacgcgcc gcgcacgtcg accagactgc gccagctgcc
     3361 gaccagcatc tcgaccggcg ccagcgcgcg gccgaggatg atggtgcagg cgatcatcac
     3421 gcccgaggac aggttctgcg agatcaccag ccaggcgccg gcgccgagca tggtcatctg
     3481 gatcgcctgg cgcgcgaact tggtcagccc ggagaacagc gcgccgagcc ggctcgcctc
     3541 gcgctgctcg cgcagcgcgg tctcgttcag cgcgctccag cgccgcgtca ccgcctcgcg
     3601 catgccgagc gcgtcgacgc tttccgccga gcgcaccgcg gcgtcgatga agcggccgct
     3661 gcgccggctt tcggcctgca cgcgctccag cggctggcgc gtgaagcgct cgttcgccca
     3721 ggcgaggccg agcatcagga gcgcgccgcc gatcgcgagg atgccgagca gcgggtggaa
     3781 cagccagatc agcgcgagga acaggggcag ccagggcgtg tcgaacagcg cgatcaccgg
     3841 cgcgccgacc aggaagccgc gcagcgtcgc cacgtcgcgc aggccgttca ggtgctcggc
     3901 gccgccggtg cgcgcggtga gcgcaagcag gctgcgcagc acgtccgggc cgagcgagcg
     3961 gtcgagcgcc aggccgcagg cggcgagcag ctgcgcgcgc agcacgtcga gcagcgacat
     4021 catggctagc gcaagcaccg cgccgaagga cagcatcacc agcgtctcct cgctgcggct
     4081 ggcgagcacg cggtcgaaga cctgcagcat gtacagcgcc ggcacgagca acaggacgtt
     4141 gatggcgagg ctgaaagcac ccgcgtaggc gaaataccgc agcaggctgc cgaccaggtt
     4201 gcgcatgaaa gattatagtc gcatcaatga gttgggcttt ccatggcccg atcaggggag
     4261 tgcgggcgca ttagttgcat cggcgccgtg catgaaacca gcccagatcg aagcgctcgc
     4321 cggcaccagc ctgctcgtgt tctccgcctt ccggcgcgga ccgctcggcc tgctcgcgtt
     4381 cctcgcggcg accggcatga tcgtccacgc cgcgacgcag cgcgccgtcg aagggttcaa
     4441 gtagcccggc gcggattcgt catcgggtaa gctccgatga tgaaaagcct gaccgcaatc
     4501 gttctgctgg tcgcgggcga ggccttcgcg caggcctatc cggcgaaggc ggtgaccttc
     4561 atcgtcccct tcgccccggg cggcggcacc gacatcacgg cgcgcaccgt ttccgccaag
     4621 ctcgcgcaga tctgggcgcg ctcgggcccg gtttcgacga tcgtcgacaa ccgcggcggt
     4681 gccggcggca tcctcggcgc cgacgcggtg gcgaaggcga agcccgacgg ctacaccctg
     4741 ctcatcgcca acgtcggcac gcagtccatc aacccatcgc tctatcccaa gctgccgtac
     4801 aaccccgaca ccgccttcgc gccgatctcg ctggtctgcg agctgccctt cgtgctgatg
     4861 gcgagcccga agttcgcgcc gagctcggtg aaggagctgg tcgcctacgc cagggccaat
     4921 ccgggcaagg tcacctatgc gagctcgggc aacggcggct cgccgcacct caccgcggag
     4981 ctgttccaga tgctcaccgg gacgcagatg acgcacgtgc cctacaaggg cggcggcccg
     5041 gcgatgatcg acctcatggc gggccacgtc gacctgctgt tcgcttcggt gctcgagggc
     5101 tccgggcaca tcaaggcggg ccggctcaag ggcctggccg tcacgcacgc caagcgcaat
     5161 gcggcgctgc ccgatgtgcc caccatcgcg gaggccggcg tgaa
//
LOCUS       DASYJA010000118         1512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1109133, whole genome shotgun sequence.
ACCESSION   DASYJA010000118 DASYJA010000000
VERSION     DASYJA010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109133"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1512
                     /locus_tag="VGO02_02535"
     CDS             <1..>1512
                     /locus_tag="VGO02_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEV7476594.1"
                     /translation="AVDWKHVHSYMHCWRHKTPVILRATTQWFASMDKPVAGRSLRQS
                     ALAGIEKTEFFPSWGQARLHGMIEHRPDWTLSRQRQWGVPMPFFLHKETGALHPRTQA
                     LLEEVAKRVEQGGIEAWQSVTAEELLGSESSSYEKSRDTLDVWFDSGSTHFTVLRRSH
                     REESTYPADMYLEGSDQHRGWFHSSLLTACMLDGHPPYRSLLTHGFVVAGDGRKMSKS
                     LGNVMAPQKISETLGAEILRLWVASTDYSGELSISDEILKRVVESYRRIRNTLRFLLA
                     NTSDFDPAKHAVPAEQMVEVDRYALALARNMQDAAAADYERYQFHLVMQRIQAFCSED
                     LGAFYLDISKDRLYTCGADSHARRSAQTAIHHITHSLIRLMAPILSFTAEESWQVFGR
                     EGSVFEKTWYALPDAKLDAAQFDSWGAVRRLREVVTKGIEEKRVLKQVGSGLAAELDI
                     RASGPEYDALARLGEELRYVFITSRATVHRAEGPVAVDVIPSANQKCERCWHYR"
BASE COUNT          274 a          495 c          491 g          252 t
ORIGIN      
        1 cgcggtcgac tggaagcacg tgcacagcta catgcattgc tggcggcaca agacgccggt
       61 gatcctgcgc gccaccacgc agtggttcgc gtcgatggac aagcccgtcg ccggccgatc
      121 ccttcgccag agcgccttgg cgggaatcga gaagactgaa ttcttccctt cctggggcca
      181 ggcgcgcctg cacggcatga tcgagcaccg cccggactgg acgctgtcgc gccagcgcca
      241 gtggggcgtg ccgatgccgt tcttcctgca caaggaaacc ggggcgctgc atccgcgcac
      301 gcaggcgctg ctcgaggagg tcgcgaaacg cgtggagcag ggcggaatcg aggcttggca
      361 gagcgtcacg gccgaggaac tgctgggttc tgaaagctct tcctatgaaa aaagccgcga
      421 taccctggac gtctggttcg actccggcag cacgcacttc accgtgctgc ggcgctcgca
      481 cagggaagag tcgacgtatc cggccgacat gtacctggag ggctccgacc agcatcgcgg
      541 ctggttccac tcgtcgctgc tcactgcctg catgctcgac gggcacccgc cgtaccgcag
      601 cctgctcacg cacggcttcg tggtcgccgg cgacggccgc aagatgtcca agtcgctcgg
      661 caatgtgatg gcgccacaga agatttcgga gactcttggc gccgagatcc tgcgcctctg
      721 ggtggcgagc accgactact cgggagagct ctccatctcc gacgagattc tgaagcgggt
      781 ggtcgagagc tatcggcgca tccgcaacac actgcgcttc ctgctcgcca atacctcgga
      841 tttcgacccg gccaaacacg ccgtgccggc cgagcagatg gtggaggtcg accgctatgc
      901 gctcgcgctc gccagaaaca tgcaggatgc ggcggcggcc gactacgagc gctaccagtt
      961 ccacctggtc atgcagcgca tccaggcttt ctgctccgag gacctgggcg ccttctatct
     1021 cgatatctca aaggaccgtt tgtatacctg cggcgccgac tcgcacgcgc ggcgttccgc
     1081 ccagaccgcg atccatcaca tcacccacag cctgatccgc ctgatggcgc cgatcctgag
     1141 ctttaccgcg gaggaatcct ggcaggtgtt cggccgcgag ggcagcgtgt tcgagaagac
     1201 ctggtacgcg ctaccggacg cgaagctcga tgcagcgcag ttcgacagct ggggagcggt
     1261 gcggcgcctg cgcgaggtgg tgaccaaggg catcgaggag aaacgcgtcc tcaagcaggt
     1321 gggttccggt ctcgcggcgg agctcgacat ccgggcctcg ggccccgaat acgacgcgct
     1381 cgcaaggctg ggcgaggaac tgaggtacgt attcatcacc tcacgcgcga ccgtgcatcg
     1441 cgccgaaggt ccggttgcgg tcgatgtgat accgagcgcc aaccagaagt gcgagcgctg
     1501 ctggcactac cg
//
LOCUS       DASYJA010000119         2812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2742121, whole genome shotgun sequence.
ACCESSION   DASYJA010000119 DASYJA010000000
VERSION     DASYJA010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2742121"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1089)
                     /locus_tag="VGO02_02540"
     CDS             complement(94..1089)
                     /locus_tag="VGO02_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEV7476595.1"
                     /translation="MSGEPLLRVEGLRKHFALTKGILFARTLGYIKAVDDVSFEIRAG
                     QTLGLVGESGCGKTTTSRMILNLEAPTEGRILLEGEPIHALQGDALRAYRAKVQAVFQ
                     DPWSSLNPRMKVGRTIAESLIVNASRDRGRGDQARIDGRVRELLLQVGLRPEQAQQYP
                     HEFSGGQRQRVALAAALASRPKLIVLDEPVSALDVSIRAQMMNLLKDIQAQDDVAYLL
                     VAHDLATVRHMADHTVVMYLGKIVEQAPTAALFADVRHPYTRALFSAVLVAGAGGQEE
                     EIVLKGEVPSPLNPPSGCHFHPRCPWVMPRCSQQEPALREVSPGHAVACHLFDNA"
     gene            complement(1086..2075)
                     /locus_tag="VGO02_02545"
     CDS             complement(1086..2075)
                     /locus_tag="VGO02_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7476596.1"
                     /translation="MTPDKRGETVLEVRDLHTHFFLRRGVVKAVDGVSFSLRRGEVLG
                     LVGESGCGKSLTALSLMRLLPKGGARTIKGEVLLDGQNILECTPAEMREIRGRRISMV
                     LQDPQTSLNPVFTIGDQLREAILRRKRSTRAEVMKEAVAALRRVEISAPEQRIGQYPH
                     QMSGGMKQRVVGAIAISGEPGVLIADEPTTALDVTIQLQYLKLLKRLQTETGMAILFI
                     THDFGVVGRMCDRVAVMYAGRIVECGPVRQIFEAPQHPYTRALIASVPRMSGPLGRLT
                     TIEGQPPSLMDLPVGCRFASRCSLVEQRCLDAYPGTVRVGEEHTADCWKVAPA"
     gene            complement(2072..>2812)
                     /locus_tag="VGO02_02550"
     CDS             complement(2072..>2812)
                     /locus_tag="VGO02_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7476597.1"
                     /translation="SPIDQTLRDKLLPPAWLAGGSDKYFLGTDAFGRDILSRLIYGAR
                     VSLLVAVLALTAGGGVGLAVGIVAGYLGGAVDSVLMRLVDAAFTFPAILFALLLAVTM
                     GQGLGTLVIAITLLLWASFARVIRGEVLSLRHRDFVALARVRGCSAPRIMLTHILPNV
                     LNTFMVLVTLNIGVVIIAEATLSFLGAGIPPPTPTWGLMISEGRGRIADAWWVALIPG
                     IAITLMVLSVNLFGDWLRDRLDPRLRQL"
BASE COUNT          461 a          962 c          983 g          406 t
ORIGIN      
        1 ggcgctgtaa acagcgccga cgtgggccac ctcccccgtc gcgcctttga aggcgctgag
       61 acggggaggc gaatgaacga tgtgatcggc gggctaggca ttgtcgaaca ggtggcaggc
      121 cacggcatga ccgggcgata cttcgcgcag cgccggttcc tgctgcgagc agcgcggcat
      181 gacccaaggg cagcgtggat ggaagtggca gccggacggc ggattgagtg gcgagggcac
      241 ctcgcccttg agcacgatct cctcctcctg cccgcccgct cccgccacca gcacggccga
      301 gaacagggcc ctggtgtagg gatggcgaac gtccgcgaac agggcggcgg tcggcgcctg
      361 ctcgacgatc ttgccgagat acatcaccac cgtgtggtcg gccatgtggc gcacggtggc
      421 gaggtcgtgc gccaccagca ggtaggccac gtcgtcctgc gcctggatgt ccttcaggag
      481 gttcatcatc tgggcgcgga tcgacacgtc gagcgccgag accggctcgt cgagcacgat
      541 cagcttgggt cgcgaagcca gcgcggcggc aagcgccacg cgctggcgct gcccgccgct
      601 gaactcgtgc ggatattgct gggcctgctc gggccgcaag ccgacctgga gcaggagctc
      661 gcgcacccgc ccgtcgatcc gcgcctggtc gcccctcccc cgatctctgg aggcgttgac
      721 gatcagcgat tcagcgatgg tgcgccccac cttcatgcgc gggttgagcg acgaccaggg
      781 atcctggaac accgcctgca ccttggcgcg gtaggctcgc aacgcgtcgc cctgcagcgc
      841 gtggatcggc tcgccctcga gcaggatgcg gccctcggtc ggcgcctcga gattgaggat
      901 catgcgcgac gtcgtggtct tgccgcagcc cgactcgccc accaggccca gtgtctggcc
      961 ggcgcggatc tcgaaggaga cgtcgtcgac cgccttgatg tagccgagcg tgcgcgcgaa
     1021 caggatgccc ttggtgaggg caaaatgctt gcgcagcccc tcgacgcgca gcagtggctc
     1081 gccgctcatg ctggtgccac cttccagcaa tccgccgtat gctcctcgcc gacgcggacc
     1141 gtgccgggat aggcgtccag gcaacgctgc tcgacgaggg agcagcgcga ggcgaagcgg
     1201 cagcccaccg gcaggtccat cagcgacggc ggctggccct cgatggtggt gagccggccg
     1261 agcggaccgc tcatcctggg caccgaggcg atcagcgcgc gggtgtaggg atgctgcggc
     1321 gcctcgaaga tctgccgcac cgggccgcac tcgacgatgc ggccggcata catcaccgcc
     1381 acgcggtcgc acatgcggcc gacgacgccg aaatcgtggg tgatgaacag gatcgccatg
     1441 ccggtctcgg tctgcaggcg cttgagcagc ttgagatact ggagctggat cgtgacgtcg
     1501 agcgcggtgg tcggctcgtc ggcgatcagc acccccggct cgccgctgat ggcgatcgcg
     1561 cccaccacgc gctgcttcat gccaccgctc atctggtgcg gatactggcc gatgcgctgt
     1621 tcgggcgccg aaatctcgac tctgcgcaag gccgccaccg cctctttcat cacctcggcg
     1681 cgggtgctgc gcttgcggcg caggatcgcc tcgcgcaact ggtcgccgat cgtgaagacc
     1741 ggattgagcg aggtctgcgg gtcctgcagc accatcgaga ttcggcggcc gcggatctcg
     1801 cgcatctccg cgggcgtgca ctccaggatg ttctggccgt cgagcagcac ctcgcccttg
     1861 atggtgcggg cgccgccctt gggcagcagg cgcatcagcg acaatgccgt caggctcttg
     1921 ccgcagcccg attcgcccac caggcccagc acttcgccgc gccggagcga gaagctcacg
     1981 ccatcgaccg ccttcacgac gccgcggcgc aggaagaaat gggtgtgcag gtcgcggacc
     2041 tcgaggacgg tctcgcccct cttatctggc gtcatagctg gcgcagcctc gggtcgagcc
     2101 ggtcgcgcag ccagtcgccg aacaggttga ccgagagcac catcagcgtg atggcgatgc
     2161 ccgggatcag cgccacccac caggcatcgg cgatgcgccc gcgcccctcg gagatcatca
     2221 ggccccaggt cggcgtcggc ggcgggatac cggcgccgag gaagctcagc gtcgcctcgg
     2281 cgatgatgac gacgccgatg ttgagcgtga ccaggaccat gaaggtgttg agcacgttgg
     2341 gcaggatgtg ggtcagcatg atgcgcgggg ccgagcagcc gcgcacccgg gcgagcgcca
     2401 cgaagtcgcg atgccggagc gacagcacct cgccccggat gacgcgcgcg aagctcgccc
     2461 acaagagcag ggtgatggcg atcaccagcg tgcccaggcc ctggcccatg gtgacggcaa
     2521 gcagcagcgc gaacaggatg gccgggaagg tgaaggcggc gtcgaccagg cgcatcagca
     2581 cgctgtcgac cgcgccgccc aggtagcccg cgacgatgcc gacggccagg ccgacgccgc
     2641 cgcccgcagt cagcgccagc accgccacca gcaggctgac ccgcgcgccg tagatcaggc
     2701 ggctcaggat gtcgcgaccg aaggcgtcgg tgccaaggaa gtacttgtca ctaccgcccg
     2761 ccagccaggc cggcggcagc agcttgtcgc gcagggtctg gtcgatcggc ga
//
LOCUS       DASYJA010000120         3856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_109766, whole genome shotgun sequence.
ACCESSION   DASYJA010000120 DASYJA010000000
VERSION     DASYJA010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109766"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VGO02_02555"
     CDS             <1..712
                     /locus_tag="VGO02_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7476598.1"
                     /translation="LKIILAGCAIAAVTYFPLFAGLTHYINPGLEEFSAKTPISVTAD
                     PASCQFHIFVGPWSKFSDCDRTKDYLTKQGLSFKSLNGAPGSAVVTTIGDGASAVKIE
                     GPDQKVIGAALAKAGYAPAPDKSRINWVMAEVILVIMVIYVTMVYGPIAAFLVELFPV
                     RIRYTSMSLPYHIGNGWFGGMLPLTATAMVAATGDIYFGLWYPIVVALMTLVIGTLFL
                     KEVKDRDIHHNESVLDAR"
     gene            complement(770..1444)
                     /locus_tag="VGO02_02560"
     CDS             complement(770..1444)
                     /locus_tag="VGO02_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7476599.1"
                     /translation="MRILVAEDDPSVAEGLMRSLRQAGYAVDCAKTGDEADTALEADE
                     FDLLILDIGLPKKSGLEVLKRLRSRGSKVPVLILTARDSLNDRVTGLDAGADDYLAKP
                     FELAELAARVRALARRGMAGGLALISHGALTYDQIGRVARLNDDPIELSARETGLLEV
                     FLHRAGRLVSKDQLVNHLCEWGEEVSGNAIEVYVHRLRKKLEPGGVRIVTVRGLGYSL
                     EKPDTR"
     gene            1502..1592
                     /locus_tag="VGO02_02565"
     tRNA            1502..1592
                     /locus_tag="VGO02_02565"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1536..1538,aa:Ser,seq:gga)
     gene            1796..1894
                     /gene="ffs"
                     /locus_tag="VGO02_02570"
     ncRNA           1796..1894
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VGO02_02570"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1900..3435
                     /gene="dnaX"
                     /locus_tag="VGO02_02575"
     CDS             1900..3435
                     /gene="dnaX"
                     /locus_tag="VGO02_02575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEV7476600.1"
                     /translation="MSYQVLARKWRPRDFGSLVGQEHVVRALRHALEQKRLHHAYLFT
                     GTRGVGKTTLARILAKCLNCETGITPEPCGKCSACVEIDAGRFVDLLEVDAATNTRVE
                     EMRQLLETAQYTPTRGRFKVYVIDEVHQLSGHAFNSMLKTLEEPPEHVKFILATTDPQ
                     KIPVTVLSRCLQFNLKQMPPAAIVAHLAKVLTAEKIEHEADALPVIARAASGSMRDAL
                     SLLDQAIAHGGGRVAAAGVSEMLGTIDQGYLVRLLECVAGGDAVGAAGIADDMQARSL
                     SFDGALSDLAGLLLRVALVQSAPGALDDAAERARIEDLARRLDRESVQLYYQIALQGR
                     EDLPLAPDEHAGFLMTVLRMLAFRPEGKGEKLPAAGKVPAAVSKPSPKAADAGGWPEL
                     ASQLQLAGAARELARNAELRRREGNLFDLVVPKSKAFLADRVHQDKLKAALEQHLGAA
                     VTVKLSVGEVSGASAAAIEAGDRDAKRAAATQAVQSDGFVQDLVNMFDGKVVDSTIRS
                     PEK"
     gene            3454..3777
                     /locus_tag="VGO02_02580"
     CDS             3454..3777
                     /locus_tag="VGO02_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004550340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV7476601.1"
                     /translation="MKGNIGQLMKQAQMMQENMRRMQESLATLEVEGQSGAGMVKVQM
                     TCKYEVKRVSIDPSLVGDDREMLEDLVAAAFNDASHRVEATIAEKMSGMTAGLGLPAG
                     FKLPF"
BASE COUNT          690 a         1235 c         1282 g          649 t
ORIGIN      
        1 gctgaagatc atcctggcgg gctgcgctat cgcggcggtc acctacttcc cgctgttcgc
       61 cgggctgacg cactacatca acccggggct ggaggagttt tcggcgaaga cgccaatctc
      121 ggtgacggcc gatccggcga gctgccagtt ccacatcttc gtcggcccgt ggagcaagtt
      181 ctccgattgc gaccgcacca aggactacct gaccaagcag ggcctgtcct tcaagtcgct
      241 caacggtgca cccggctcgg cggtggtcac caccatcggc gacggcgcca gcgcggtgaa
      301 gatcgagggc ccggaccaga aggtgattgg cgcggcgctg gccaaggccg gctatgcgcc
      361 cgcgccggac aagagccgca tcaactgggt gatggccgag gtgatcctcg tcatcatggt
      421 gatctacgtg acgatggtgt acggaccgat cgccgcgttc ctggtggagc tgttcccagt
      481 aaggatccgg tacacgtcga tgtctcttcc ctaccatatc ggcaatggtt ggttcggtgg
      541 catgctgccg ctgacggcca cggcgatggt cgccgcgaca ggggacattt acttcggcct
      601 ctggtacccg atcgtggtag cgctgatgac gctggtcatc ggcacgctgt tcctgaaaga
      661 ggtcaaggac cgcgacatcc atcacaacga gtcggtgctc gacgcgcgct gacgtttttc
      721 aaggcaagaa aaaaaggccc gcttcgcgcg ggcttttttt tgccttgggc taccgcgtgt
      781 ccggtttttc caggctgtaa cccaggcccc ggacggtgac gatgcgcacg ccgcccggtt
      841 ctagcttctt ccgcaggcgg tggacgtaca cctcgatcgc gttccccgat acttcctcgc
      901 cccattcgca aaggtgattg accagctggt ccttcgatac caggcgcccc gcgcgatgca
      961 ggaatacctc gagcagaccc gtttcccgcg cggagagctc gataggatcg tcgttcaaac
     1021 gtgcgacacg gccgatctgg tcgtaggtga gcgcgccatg gctgatgagc gccaggccgc
     1081 ccgccatgcc gcggcgcgcg agcgcgcgca cccgcgcggc caactcggcg agttcgaaag
     1141 gcttggccag atagtcgtcg gcgcccgcgt ccaggccggt gacgcggtcg ttcaggctgt
     1201 cgcgcgcggt gaggatcagt accggtacct tggagccgcg cgagcgcagg cgcttcagca
     1261 cctccagccc ggatttcttc ggcaggccga tgtcgaggat cagcagatcg aactcgtcgg
     1321 cctcaagagc ggtgtccgct tcgtcgccgg ttttagcgca gtccaccgcg tagcccgcct
     1381 ggcgcagcga gcgcatcaag ccctcggcca cgcttgggtc gtcctcggca accaggatgc
     1441 gcacgctctc tcctcctggg cagtgtagca aagggaggct ggggtaaaat ccgccgctcg
     1501 cggagagatg gccgagcggc ttaaggcgca cgcttggaaa gcgtgtgtac gttaatagcg
     1561 taccgtgggt tcgaatccca ctctctccgc cacttcggat ttccgaaccc cgcaaaagaa
     1621 aacgcccggc agcttgcgct ggcagggcgt ttaaaccacc gcacggttaa gcgcgatgga
     1681 ggaggatacg ttcggaaaaa agaatagcgc tcgccggctc taacgccctg actggaagct
     1741 tacggtcggc ttactttgcg agttgtttcg ctctcgcatg acttacaatg gggtcggcgg
     1801 gcctccccgc attgcaggcc ggtgaatctg gtcaggtccg gaaggaagca gccacagctg
     1861 gaccctgcaa gtgccggggg tcaggctcgc cactaataca tgagctatca ggtacttgca
     1921 cgcaaatggc ggcctaggga tttcggcagc ctggtaggtc aggagcacgt cgtccgggcg
     1981 ctgcggcatg cgctggagca aaagcggctg catcacgcgt atctctttac cgggacccgc
     2041 ggcgtcggca agaccacgct cgcccgcatt ctcgcgaaat gcctcaattg cgagaccggt
     2101 attacccccg agccctgcgg caagtgctca gcctgcgtcg agatcgacgc aggccgcttc
     2161 gtcgacctgc tcgaggtcga cgccgcgacg aacacccggg tggaagagat gcgccagctg
     2221 ctggaaaccg cgcagtacac gccgacccgc ggccgtttca aggtgtacgt gatcgacgaa
     2281 gtccaccagc tctcgggcca tgccttcaac tcgatgctga agacgctcga ggagccgccc
     2341 gagcacgtca agttcatcct ggcgacgacc gacccgcaga agatcccggt gacggtgctc
     2401 agccgctgcc tgcaattcaa cctgaagcaa atgccgcctg cggccatcgt ggcgcacctc
     2461 gccaaggtgc tcaccgccga gaagatcgag cacgaggccg atgcgctgcc ggtgatcgcg
     2521 cgcgcggctt cgggcagcat gcgcgacgcg ctttcacttc tcgatcaggc gatcgcgcac
     2581 ggcggcggcc gggtggccgc tgccggggtg agcgagatgc tcggcaccat cgaccagggc
     2641 tacctcgtgc gcttgcttga gtgtgtagct ggcggcgatg cggtgggcgc ggccgggatc
     2701 gccgacgaca tgcaggcgcg cagcctgtcg ttcgatgggg cgctttccga cttggcgggc
     2761 ctgctgctgc gcgtcgcgct cgtccagtcg gcgccagggg ccctagacga cgctgccgag
     2821 cgcgcccgca tcgaggacct ggcacggcgg ctcgaccgcg agtcggtgca gctttactac
     2881 cagatcgccc tgcagggaag ggaagacctg ccactcgcgc ccgacgagca cgccggcttt
     2941 ctcatgacgg tgctgcgcat gctcgccttc cgcccggagg gcaagggcga aaagctgccg
     3001 gctgccggca aggttccggc cgcggtttcg aagccctcgc cgaaggcggc cgacgccggc
     3061 ggctggccgg agctcgccag tcagctgcag ctcgccggcg ctgcgcgcga gctcgcgcgc
     3121 aacgccgagc tgcgtcgccg cgaaggcaac ctcttcgacc tggtggtgcc gaagtcgaag
     3181 gcgtttctcg ccgaccgggt ccaccaggac aagctcaagg cggcgctcga gcagcacctc
     3241 ggtgccgcgg tgacggtgaa gctctcggtc ggagaagtga gcggcgcttc ggcggccgcg
     3301 atcgaggcgg gcgaccgcga cgcgaagcgg gctgcggcaa cgcaggccgt gcagtccgac
     3361 ggcttcgtgc aggacctggt caacatgttc gacggcaagg tcgtcgactc aaccatccgg
     3421 tcgcctgaaa aatgagggag aaatgagcca gccatgaaag gcaacatcgg acagctgatg
     3481 aagcaagcgc agatgatgca ggagaacatg cgccgcatgc aggaaagcct cgccaccctc
     3541 gaggtcgaag gccagtcggg cgccggcatg gtgaaagtgc agatgacctg caagtacgag
     3601 gtgaagcgcg tgtcgatcga tccttcgctg gtcggcgacg accgcgagat gctcgaggac
     3661 ctggtcgccg ccgcgttcaa cgacgcctcg caccgcgtcg aggcgaccat cgccgagaaa
     3721 atgagcggca tgacggccgg cctcggattg ccggcgggat tcaagctgcc gttttgaaaa
     3781 gcaaagacgc cgtcccgacc gcgaaggggc tcgagctcct ggtcgaggcg ctgcgcgtgc
     3841 tgccgggagt gggacc
//
LOCUS       DASYJA010000121         2490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_474286, whole genome shotgun sequence.
ACCESSION   DASYJA010000121 DASYJA010000000
VERSION     DASYJA010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474286"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VGO02_02585"
     CDS             complement(<1..420)
                     /locus_tag="VGO02_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEV7476602.1"
                     /translation="MNKPVEAAAATVTGAEAAVEMLKAHRVEIIFGLCGDTSLPLYDA
                     LARLPHGMKHVLTRDERHAAYMADGYARVSGKVGVCEGPSGGGATYILPGLVEANESS
                     VPVLAINTDVSVSSRGRFTLTELDQKTMMRPLTKWNAV"
     gene            complement(430..810)
                     /locus_tag="VGO02_02590"
     CDS             complement(430..810)
                     /locus_tag="VGO02_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476603.1"
                     /translation="MLTLVFAEPRPGPVVTRGPFPEVRADAICLRAGNGGEVIARHEN
                     HCWRIGAREFFRIDCEGPVRVYFEDPGGQHSEVLGPFFHFSSADGLAYGDGEICGHVD
                     VDAGTWFSHREQAHWKEMVVVPAG"
     gene            825..1679
                     /locus_tag="VGO02_02595"
     CDS             825..1679
                     /locus_tag="VGO02_02595"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7476604.1"
                     /translation="MQVAERQKPPPGELYLDHVAHFVPDLGAAARLLESLGFTPTPES
                     AHRAQGAPAGTSNRCLMFEEGYVEILAPTLKTPNAERVRAHMARYDGVHLACFGTPDA
                     AFEHQRLAGQGFEPEPLVDLQRTVDGGETVRFSVVYVPPGRMPEGRVQYVQQLTPEVI
                     WKDRFVEHANGVTGLSAVYVAADEPAEAGARWARFSGLLPRREGDLVCLQAARGKVLI
                     GKRTNLFANAPAAPALAGYALSCRHPEAFVARCSKLGLKVQGLSASGMAAGHAITLPP
                     SLGGTWLV"
     gene            1708..>2490
                     /locus_tag="VGO02_02600"
     CDS             1708..>2490
                     /locus_tag="VGO02_02600"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7476605.1"
                     /translation="MKVFKTTALIALAFAASAASAQTVKIGFITSYSGLNGNLGPYME
                     RAVRLYLKQHEKELGGVKVELITRDDTGPNPDKARQLAQELVVRDKVDLLAGVIFTPN
                     AMAMAPIATEAKVPFVIMNAGTAVITTRSPYMVRTSFTLWQSSYPLGQWAAKKYKTAY
                     SLVSDFGPGHDCEEAFSKGFEEGGGKILGKVRVPLQNPDWAAYMQRVKDAKPDVLNVF
                     IPAGKTATAVMKNFSDLGLDKAGIKLIGPGDITTDEELPNMGD"
BASE COUNT          431 a          847 c          826 g          386 t
ORIGIN      
        1 caccgcgttc cacttcgtca gcggccgcat catggtcttt tgatccagct cggtcaaagt
       61 aaaacgacca cgggacgaaa cagagacatc cgtgttgatg gcgagcacgg gaacggaaga
      121 ctcgtttgcc tcgaccaggc cgggcaggat gtaggtggcg ccgccgcccg agggcccttc
      181 gcagacgccg accttgccgc tgacgcgcgc gtagccgtct gccatgtagg cggcgtggcg
      241 ctcgtcgcgc gtcagcacgt gcttcatgcc gtgcggcagg cgcgcgagcg cgtcgtagag
      301 aggcaggctg gtgtcgccgc acagcccgaa gatgatctcg acgcggtgcg ccttgagcat
      361 ctccaccgcc gcttcggcgc ccgtgaccgt cgccgccgcg gcctcgacgg gcttgttcat
      421 gcgggtatct taacccgcgg gcaccaccac catttccttc cagtgcgcct gctcgcggtg
      481 gctgaaccag gtgccggcgt cgacgtcgac gtgcccgcag atctcgccgt cgccgtaggc
      541 caagccgtcg gccgaagaga aatggaagaa cggccccagc acctcggaat gctgcccgcc
      601 cgggtcctcg aagtacaccc gcacggggcc ttcgcagtcg atgcggaaga attcgcgcgc
      661 gccgatgcgc cagcaatgat tctcgtgccg ggcgatcacc tcgccgccat ttcccgcgcg
      721 cagacatatt gcgtcggcgc ggacctccgg gaagggcccc cgcgtaacca ccggcccagg
      781 acgcggctcg gcgaagacga gcgtcagcat gggacaattt ccatatgcag gtagccgagc
      841 gccaaaaacc gcctccgggg gagctatacc tcgaccatgt cgcacatttc gtgcctgacc
      901 tgggcgcggc ggcgaggctg ctcgaatctc tcggcttcac gccgacgccc gagtcggcgc
      961 accgcgcaca aggcgcgccg gcgggcacct cgaaccgctg cctgatgttc gaggagggct
     1021 atgtcgagat cctcgcgccg acgctcaaga cgccgaacgc ggagcgcgtg cgcgcgcaca
     1081 tggcgcgcta cgacggcgtg cacctcgcct gcttcggcac gcccgacgcc gccttcgagc
     1141 accagcgcct cgccggccag ggcttcgagc ccgagccgct ggtcgacctg caacggaccg
     1201 tcgacggcgg cgagaccgtt cgcttcagcg tggtctacgt gccgcccggg cgcatgccgg
     1261 aagggcgcgt ccagtacgtc cagcagctca cgcccgaggt catctggaag gatcggttcg
     1321 tcgagcatgc caacggcgtc accggccttt cggcggtcta cgtcgccgcc gacgaaccag
     1381 ccgaggccgg ggcgcgctgg gcgcgctttt ccggcctgct gccccgcagg gagggcgatc
     1441 tcgtctgcct gcaggcggcg cgcggaaagg tgttgatcgg aaagaggacg aacttgttcg
     1501 cgaatgcccc ggcggcgccc gctctggccg gttatgcgct gtcctgccgt catccggaag
     1561 ctttcgtggc gcgttgctcc aagctggggc tgaaagtgca ggggctctcg gcgagcggta
     1621 tggccgccgg ccatgccatt accctgccgc cctcgctcgg cggcacatgg ctcgtttaga
     1681 atccacgctt ccttaggagg ggacaaaatg aaggtattca aaactacggc gctcatcgcg
     1741 ctggcgttcg cggcgtccgc tgcgtccgcg cagaccgtca agatcggctt catcaccagc
     1801 tactccggcc tcaacggcaa cctcgggccg tacatggagc gcgcggtgcg cctgtacctg
     1861 aagcagcacg agaaggagct cggcggcgtc aaggtcgagc tcatcacgcg cgacgacacc
     1921 ggcccgaacc cggacaaggc gcgccagctc gcgcaggagc tggtggtgcg cgacaaggtc
     1981 gacctgctcg caggcgtgat cttcacgccc aacgccatgg cgatggcgcc gatcgccacc
     2041 gaagccaagg tgccgttcgt gatcatgaac gccggcacgg cggtcatcac cacgcgctcg
     2101 ccgtacatgg tgcgcacctc gttcacgctg tggcagtcga gctacccgct cggccagtgg
     2161 gcggcgaaga aatacaagac cgcctacagc ctggtgagcg acttcggccc gggtcacgat
     2221 tgcgaagagg cgttcagcaa gggcttcgag gaaggcggcg gcaagattct cggcaaggtg
     2281 cgcgtgccgc tgcagaaccc tgactgggct gcgtacatgc agcgcgtcaa ggacgcgaag
     2341 cctgatgtac tgaacgtgtt catcccggcg ggcaagaccg ccaccgcggt gatgaagaac
     2401 ttttcggacc tgggcctgga caaggccggc atcaagctga tcggcccggg cgacatcacc
     2461 accgacgagg agctgccgaa catgggcgac
//
LOCUS       DASYJA010000122         3726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2652371, whole genome shotgun sequence.
ACCESSION   DASYJA010000122 DASYJA010000000
VERSION     DASYJA010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2652371"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..570
                     /locus_tag="VGO02_02605"
     CDS             151..570
                     /locus_tag="VGO02_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEV7476606.1"
                     /translation="MKKSGASQGQSAAELISKRIAELGDWRGETLGRMRKLIKQADPD
                     VVEEWKWMGTPVWSHDGIICTGESYKSVVKLTFAKGASLKDPARLFNSSLDGNVRRAI
                     DIHEGEEVDGSAFKALVRQAVALNSSGKSKSSKKAKS"
     gene            585..1199
                     /locus_tag="VGO02_02610"
     CDS             585..1199
                     /locus_tag="VGO02_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV7476607.1"
                     /translation="MQTVSLKVLAAVFVFASSTFALGQARFPDIPRDQMTEAQKHVYD
                     AIASGPRAGVRGPFGPLLRSPELTDRVQKLGEYLRFNSSLPPRLNEMAILINARFWES
                     KYEWFAHKPLAVKGGLAEAITADLALNKRPGNMKPDEEVVYDLCTALHKTHFVDDALF
                     KRAVGALGERGVVDLVAVSGYYTLVSMVLNVAEIPLPAGASSPW"
     gene            1258..2043
                     /locus_tag="VGO02_02615"
     CDS             1258..2043
                     /locus_tag="VGO02_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEV7476608.1"
                     /translation="MAAAWLVCFVATLLAALPAAAQQRPASAGPADPKLIEDLITANR
                     ILYQQGVVDAMGHVSVRHNKDPNRYLISLARAPELVTAADIMEYDLDSNPVRDPGRRQ
                     YSERYIHGEIYKLRPDVNAVVHNHSPGVTPFSVSSVQLRAVAHSGGFMADGVPVWEIR
                     EAGGMTDMLVSNAKFGHSLAEKLGERAAILMRGHGAAVTGKSLQYVVGRSVYLEVGAR
                     LQAQAMALGGTVNYLDPEEGRKIEARRDYIRSWEMWKRQAMGK"
     gene            complement(2051..2578)
                     /locus_tag="VGO02_02620"
     CDS             complement(2051..2578)
                     /locus_tag="VGO02_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012406068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEV7476609.1"
                     /translation="MSDLVVVTYKGEDTADQVLNKLRELQKEYLVDLEDAVVAVRDSK
                     GKVRIKQAVPLVRISAVSGLAWGGLFGLLVGLLFLNPLLGWLSGAAMGAGAGALSGAL
                     ADYGINDDFIKDIGKSLEPGTSAIFMLVRRMTYDKVLPELKRFGGTILKTSLSNEQEE
                     RLRRALEDASEAVAA"
     gene            2717..3337
                     /locus_tag="VGO02_02625"
     CDS             2717..3337
                     /locus_tag="VGO02_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEV7476610.1"
                     /translation="MDAAISILSIAGAVTLGAMSPGPSFVMVARTAVASSRADGLAAA
                     LGMGVGGVLFACAALLGLHMILTAVPWLYLGLKLVGGAYLIYLGCMIWRGARRPLQIG
                     EGVPSTSHRSFLLGLLTQVSNPKTAVVYASIFVALLPQEVPLAAMLVLPVAIFVIEAG
                     WYSIVALALSAPSPRAAYLRWKVWVDRAAGSAMGLLGIKLIATARS"
     gene            complement(3338..>3726)
                     /locus_tag="VGO02_02630"
     CDS             complement(3338..>3726)
                     /locus_tag="VGO02_02630"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEV7476611.1"
                     /translation="GGQIPLVFTAIASAQQYVRTSRLVGVGVPGAKRSSALPEVPTFQ
                     ESGLAGFDVSAWTGIFAPAATPRAVIERLQKELAVVLHSPFVKERYAVLGIEPVGNSP
                     EAFGAQVREDLARWEKVVRTANVRVE"
BASE COUNT          650 a         1225 c         1212 g          639 t
ORIGIN      
        1 cccggccggg aacactggcg gcagtgacgt tagtatgttc aagcacatgc ccattgagcc
       61 cgagttgcag aacatcatta atggtggcgc gccacctgac gcgcgggcgt agcaggcaat
      121 tccaggttgg ggcaaatcat aggcacatcc atgaaaaagt cgggcgcgag ccagggccag
      181 tcggcagcgg agctcatctc gaaaagaatc gccgagctcg gcgactggcg cggggaaacc
      241 ctcggcagaa tgcgcaagct cattaagcaa gcggacccgg acgtcgtcga ggagtggaag
      301 tggatgggca ctccggtctg gtcgcacgac ggcatcatct gcactggcga atcctacaaa
      361 agtgtcgtca agcttacctt cgccaagggc gcgtctctga aggatccggc ccgtctgttc
      421 aactcgagtc tcgacggaaa cgtgcgccgc gcgatcgaca tccacgaagg agaggaagtt
      481 gacgggtccg ccttcaaggc gctggttcgc caggcggtcg ccctcaacag ttccggcaag
      541 tcgaaatctt cgaagaaggc gaagtcctga gagagagggt gatcgtgcaa acagtgtccc
      601 ttaaggtgct cgcggctgtt ttcgttttcg catcttcgac ctttgctcta ggacaggcgc
      661 gcttcccgga catcccgcgc gaccagatga ctgaagcgca gaagcatgtg tacgacgcca
      721 tcgcgagcgg gccgcgtgcc ggcgtacgcg gtccgttcgg gccgcttctg cgcagccctg
      781 aactgaccga tcgcgtacag aagctcggcg agtacctgcg cttcaacagc tccctgccgc
      841 cgcggctgaa cgaaatggcc atcctgatca atgcccgatt ctgggaatca aagtacgagt
      901 ggtttgcgca caagccgctt gccgtcaaag gaggccttgc cgaggctatt acggccgacc
      961 ttgcgctgaa caagcgcccg ggcaacatga agccggatga ggaagtcgtc tacgatctat
     1021 gcacggcact gcacaagact catttcgtgg atgacgcgct cttcaagcgg gcagtcggcg
     1081 cattgggaga gcgcggcgtg gtcgatcttg tcgccgtcag cggctactac accctggtat
     1141 cgatggtact caacgtcgcg gagattccgc tgccggcagg ggcaagctcg ccgtggtagc
     1201 tgcatgacgc ccaacagcgc agaatcacgc ttccacaaaa tcagggagca tcggatcatg
     1261 gcagcagcct ggctggtctg tttcgtcgca actcttctcg ccgcgctgcc ggcggctgcg
     1321 cagcagcggc ccgcgtccgc cgggccggcg gacccgaagc tgatcgagga cctgatcacg
     1381 gcgaaccgca tcctctacca gcagggcgtg gtcgacgcca tgggccacgt cagcgtgcgc
     1441 cacaacaagg acccgaaccg ctacctcatc tcgctcgcgc gcgcgccgga gctggtgacg
     1501 gcggccgaca tcatggagta cgacctcgac agcaacccgg tgcgcgaccc gggccggcgg
     1561 cagtactcgg agcgctacat ccatggcgag atctacaagc tgcggccgga cgtgaacgcg
     1621 gtggtgcaca accactcgcc cggcgtgact ccattctcgg tgagcagcgt tcagttgcgc
     1681 gccgtcgcgc actcgggcgg cttcatggcc gacggcgtgc cggtgtggga gatccgcgaa
     1741 gcgggcggca tgaccgacat gctggtgagc aacgcgaagt tcggccactc gctcgccgag
     1801 aagctcggcg agcgggcagc gatcctcatg cgcggccacg gcgcggcggt gaccggcaag
     1861 tcgctccagt acgtcgtcgg ccgcagcgtc tacctcgagg tcggcgcgcg gctgcaggcg
     1921 caggcgatgg cgctgggcgg cacggtcaac tacctcgacc cggaggaagg ccgcaagatc
     1981 gaggcgcgcc gcgactacat ccgctcctgg gagatgtgga agcgccaggc gatgggcaaa
     2041 tagcgccggg ctacgccgcg acggcctcgc tcgcgtcctc cagcgctctg cgcagccgct
     2101 cttcctgttc gttcgagagc gaggtcttga gaatcgtgcc accgaaacgt ttcagctcgg
     2161 gcagcacctt gtcgtacgtc atccggcgca ccagcatgaa gatcgcggaa gtgcccggct
     2221 cgagggactt gccgatatcc ttgatgaagt cgtcgttgat gccgtaatcg gcgagcgcgc
     2281 cggacagggc ccctgcgccg gcgcccatcg cggcgcccga cagccagccc agcagtgggt
     2341 tcaggaacag cagcccgacc agcaggccga acagcccgcc ccacgcgagg ccgctgacgg
     2401 ccgatatgcg gaccagcgga accgcctgct tgatccgcac cttgcccttc gagtcgcgca
     2461 ccgccacgac ggcgtcctcc aggtcgacga ggtattcctt ctgcaattcc cgcaacttgt
     2521 tcagcacctg gtccgcagta tcttcgccct tgtaggtaac cacgactaga tcgctcatcc
     2581 gcttctcctt ggttgggcgc ggagccgcaa cgagggcctc gctcggtgga agctacgacg
     2641 cccggcgcgc ggtttatttc gccggcttgc tgttgtctta ccggatcgtg ctattgccct
     2701 gctaggcttg ccgggcatgg acgccgcaat ttcgattctg agcatcgccg gtgccgtcac
     2761 gctgggcgcg atgagcccgg gacccagctt cgtcatggtg gcgcgcaccg cggtggcgag
     2821 ctcgcgtgcc gacgggctcg ccgcggcgct ggggatgggc gtcggcggcg tgctcttcgc
     2881 ctgcgccgcc ttgctcggct tgcacatgat cctgacggcg gtaccgtggc tctatctggg
     2941 cctgaagctc gttggcggcg cctacctgat ctatctcggc tgcatgatct ggcgcggagc
     3001 gcgcaggccg ctgcagatcg gcgaaggcgt tccatcgacc tcgcaccgct cgttccttct
     3061 aggactgctc acgcaggtaa gcaaccccaa gaccgcagtc gtctatgcga gcatcttcgt
     3121 cgccctgctg ccgcaggaag tcccgctcgc cgcgatgctg gtcctgcccg ttgcaatctt
     3181 cgtcatcgag gcagggtggt attcgatcgt ggcgcttgcc ctgtccgcgc cctcgccgcg
     3241 cgctgcctac ctgcgctgga aggtctgggt cgatcgcgct gccggcagtg cgatggggct
     3301 gctcggcatc aagctcatcg cgactgcgag gtcgtagcta ctcgacgcgg acgttcgcgg
     3361 tcctgacgac cttctcccag cgggcgagat cctcgcgcac ctgcgcgccg aaagcctccg
     3421 gcgagttgcc caccggctcg atgccgagca cggcgtagcg ctccttcacg aacggcgagt
     3481 gcagcacgac ggcaagctcc ttctgcagcc gctcgatcac cgcgcgcggc gtggcggccg
     3541 gagcgaagat accggtccag gcggaaacat cgaacccggc aaggccgctt tcctggaagg
     3601 tcggcacctc gggcaacgcg ctcgagcgct tcgcgccggg cacgccgacg ccgaccagcc
     3661 ggctggtgcg cacgtactgc tgcgcgctcg cgatggcggt aaagacgagc gggatctgcc
     3721 cgccta
//
LOCUS       DASYJA010000123         1994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3197974, whole genome shotgun sequence.
ACCESSION   DASYJA010000123 DASYJA010000000
VERSION     DASYJA010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3197974"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1772
                     /locus_tag="VGO02_02635"
     CDS             75..1772
                     /locus_tag="VGO02_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEV7476612.1"
                     /translation="MENKGFLKAFWSLVRPYWTSEQRWRALAMLAAVVGLALMLVWLE
                     VQFNLWNNDFYNTFQDKDQAEFFRQFGKFTLLAVIYIITGVYRFYYQQMLMIEWRTWL
                     TERLLGEWTRDEVYYRMALLDRGTDNPDQRIAEDLKYFVDYTLSLALGLMSAVVTLVA
                     FIGILWMLSGPFEIAGTPIPGYLVWAALIYAVFGTWLTHLIGRPLIALNFNQQRVEAD
                     FRFALVRLRENSEGVALYRGEREEMANFRGRFGAVIANWWSLMLKQKRLGWFTTFYGQ
                     VAIIFPYVMVAPRFFSGAIQLGGIFQAASAFGQVQGSLSWFIGAYTQFATWKATVDRL
                     TGFRASIERVRSEGRTAAGERVENSQPTLALEDLEVDLPQGKPLLAATSLELKKGQDV
                     LVTGPSGAGKSTLLRVLAGIWPYWKGRIRLPHSARLLFLPQKPYLPIGTLKHAVSYPE
                     DAKRFSNEEIAEALQAVGLPQLTVDLERSENWGQVLSGGEQQRLAFARALLARPDWLF
                     LDEATAALPEDAQDALYRLLKERLPQTTLISIGHRESLAAHHPRRLTWSGQSLVPAG"
     gene            complement(1769..>1994)
                     /locus_tag="VGO02_02640"
     CDS             complement(1769..>1994)
                     /locus_tag="VGO02_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476613.1"
                     /translation="ASRSLDAIDYFLYRHTALIAQDLVRHRYDVEGLPYFAEMKRSGP
                     PEFVAEYRRNLGAMLERGNLYWALPPGQRH"
BASE COUNT          332 a          642 c          643 g          377 t
ORIGIN      
        1 gaggacatgc cccccgattg ttaacccgtg actacgcttc tgtaaactga tttgcatccc
       61 atctcaggtg cgccatggaa aacaaaggat ttctaaaggc tttttggtcc ctcgtccgac
      121 cctactggac ctccgagcag cgctggaggg cgctcgccat gctggccgcg gtggtcggcc
      181 ttgcactgat gctggtctgg ctcgaggtgc agttcaacct ctggaacaac gatttctaca
      241 acaccttcca ggacaaggac caggcagagt tctttcgcca gtttggcaag ttcaccctgc
      301 tcgcggtgat ctacatcatt accggcgtct accgttttta ctaccagcag atgctgatga
      361 tcgaatggcg cacctggctc accgagcgcc ttctcggaga atggacgcgc gacgaggtgt
      421 actaccgcat ggccctgctc gaccgtggca ccgacaaccc cgaccagcgc atcgccgagg
      481 atctcaagta tttcgtcgat tacacgctga gccttgcctt gggcctcatg tcggccgtgg
      541 tgacgctggt cgccttcatc ggcatcctgt ggatgctgtc gggcccgttc gaaatcgccg
      601 gcacgccgat ccccggctac ctggtctggg cggcgctgat ctatgcggtg ttcggcacct
      661 ggctcaccca cctgatcggg cggccgctca ttgcgctcaa tttcaaccag cagcgcgtgg
      721 aggcggattt ccgcttcgcg ctggtgcggc tgcgggagaa cagcgagggc gttgcgctgt
      781 accgcggcga gcgcgaggaa atggcgaatt ttcgcggccg gttcggcgcc gtgatcgcca
      841 actggtggtc gctcatgctg aagcaaaagc ggctcggctg gttcaccact ttctacggcc
      901 aggtcgccat catcttccct tacgtcatgg tagcgccgcg cttcttctcg ggcgccattc
      961 agctcggcgg catcttccag gccgcctcgg cgttcggcca ggtgcagggc tccctctcgt
     1021 ggttcatcgg cgcgtacacg cagttcgcca cctggaaggc gacggtcgat cgtctcaccg
     1081 gctttcgcgc ttcgatcgag cgcgtgcgtt cggaagggcg cacagccgcc ggcgagcgcg
     1141 tcgagaactc gcaaccgacg cttgcgctcg aggacctgga ggtcgacctc ccgcagggca
     1201 agccgctgct tgccgcgacc tcgctcgagc tgaagaaggg acaggacgtg ctggtaaccg
     1261 ggccctcggg tgccggcaag agcacgctgc tccgcgtgct cgcaggtatc tggccatact
     1321 ggaaggggcg tatccgcctg ccgcacagcg cgcggctgct cttcctgccg cagaagccct
     1381 acctgccgat cggcacgctg aagcacgcgg tgtcctatcc ggaggacgcg aagcgctttt
     1441 caaacgagga aatcgccgag gcgctgcagg cggtcggcct gccgcagctt acggtcgacc
     1501 tggagcgcag cgagaactgg gggcaggtcc tttcgggcgg cgagcagcag cggctcgcat
     1561 ttgcgcgcgc cctcctggcg aggcccgact ggctattcct cgacgaggcg accgccgcgc
     1621 tgcctgagga tgcgcaggat gcgctctatc ggttgctcaa ggagcggctc ccgcagacga
     1681 cgctgatctc gatcggtcac cgcgaaagtc tcgcggcgca tcatccaaga cggcttacct
     1741 ggagcgggca gtcgctggtg ccggcgggct agtggcgctg gccgggcggc agcgcccagt
     1801 agagattgcc gcgctcgagc atggcgccaa ggttgcgacg gtactcggcg acgaactcgg
     1861 gcgggccgga gcgcttcatc tcggcgaagt acggcaaccc ctcgacatcg tagcgatgcc
     1921 gcaccaggtc ctgcgcgatc agcgcggtat ggcggtacag aaagtaatcg atggcgtcca
     1981 gcgagcgcga tgcg
//
LOCUS       DASYJA010000124         3176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_201359, whole genome shotgun sequence.
ACCESSION   DASYJA010000124 DASYJA010000000
VERSION     DASYJA010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201359"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1265)
                     /gene="dxs"
                     /locus_tag="VGO02_02645"
     CDS             complement(<1..1265)
                     /gene="dxs"
                     /locus_tag="VGO02_02645"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012338492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEV7476614.1"
                     /translation="MYELLKTIDEPAQLRALERRQLGQLADELRAYVLESVSKTGGHL
                     SSNLGTVELTIALHYVFDTPEDRIVWDVGHQTYAHKILTGRREQMSRLRMLDGISGFP
                     RRAESKYDTFGTAHSSTSISAALGMAVSAKIKNEKRRCIAVIGDGAMSAGMAFEAMNN
                     AGAMDVDLLVILNDNEMSISRPVGALTNYLARLLSGRLYSTARRASEHLLKRVPGVWE
                     LARRAEEHVKGMVTPSTLFEEFGFNYIGPIDGHDLDTLIPTLDNIKKLKGPQFLHIVT
                     RKGQGYKLAEDDPIAYHGPGKFNPAEGLKSVAGGKPTYSQIFGQWLCDMAEREPRLVA
                     ITPAMREGSGMVKFSERFPARYFDVGIAEQHAVTFAAGVACEGMRPVVAIYSTFLQRG
                     YDQLIHDVAIQNLPVLFALDRGGLVGGDGA"
     gene            complement(1328..2236)
                     /locus_tag="VGO02_02650"
     CDS             complement(1328..2236)
                     /locus_tag="VGO02_02650"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEV7476615.1"
                     /translation="MNLRAAETDFQAWMGDVQARMEAALARLLPPAHAAPARLHEAMR
                     YATLEGGKRVRPLLAFAAGEVSGAPSERLEIAAAAVEMIHAYSLVHDDLPCMDDDVLR
                     RGKPTVHVEYDEATALLVGDALQSLAFQLLAESQIADDPQRQLDMVRILGAAAGSRGM
                     AGGQQIDLEATGKALSLPELEFMHIHKTGALIRAAVLLGFSCGEASAEAEKHRLDKYA
                     KAVGLAFQVVDDVLDYDASTATLGKTAGKDSKQGKPTYVSLMGLPRARELAQELRAEA
                     HAALEGLGGRGRRLGELADFIVLRKF"
     gene            complement(2217..2471)
                     /locus_tag="VGO02_02655"
     CDS             complement(2217..2471)
                     /locus_tag="VGO02_02655"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEV7476616.1"
                     /translation="MSKPSGEAKLSFEQALAELEKIVSRMESGDLSLEQALAAHKRGL
                     ELARFCQQRLESAQQQVKVLEGDVLKPLTAAVERDEPEGG"
     gene            2512..>3176
                     /locus_tag="VGO02_02660"
     CDS             2512..>3176
                     /locus_tag="VGO02_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEV7476617.1"
                     /translation="MAELGTLRQIKPTTCHLPVSWYFDPKIFELEQKHLFDAGANYVG
                     HELMVPKVGDYHTLSWTGDAKVLVRNAAGKEQGVELLSNVCRHRQSIMLQGRGNVQNI
                     VCPLHRWTYDLKGELLGAPFFEETPCVKLHSTPLVNWNGLLFAGPRDPRKDLARITTM
                     ADWDFSGYVLESVRVDEYDINWKTFVETYLEVYHVNFYHPGLGNFTDCDNFTIDCGEE
                     HSI"
BASE COUNT          505 a         1060 c         1051 g          560 t
ORIGIN      
        1 gcgccgtcgc cgccgaccag gccgccgcgg tcgagcgcga acagcaccgg caggttctgg
       61 atcgccacgt cgtggatcag ctggtcgtag ccgcgctgca ggaaggtcga gtagatcgcc
      121 accaccggcc gcatgccctc gcaggcgacg ccggcggcga aggtcactgc gtgctgctcg
      181 gcgatgccga catcgaaata gcgtgcaggg aatcgctcgg agaacttgac catgcccgag
      241 ccttcgcgca tcgccggcgt gatcgccacc aggcgcggct cgcgctcggc catgtcgcac
      301 agccactggc cgaagatctg cgagtacgtc ggcttgccgc cggccacgct cttcaggccc
      361 tccgcggggt tgaacttgcc gggcccgtgg taggcgatgg ggtcgtcctc ggcgagcttg
      421 tagccctgcc ccttgcgcgt aacgatgtgc aggaactgcg ggcccttcag cttcttgatg
      481 ttgtcgaggg tcgggatcag cgtgtcgagg tcgtggccgt cgatcggccc gatgtagttg
      541 aagccgaact cctcgaacag cgtcgagggc gtgaccatgc ccttgacgtg ctcctcggcg
      601 cggcgcgcga gctcccagac gcccggcacg cgcttgagca ggtgctcgct cgcgcggcgc
      661 gccgtcgagt acagccgccc ggacagcagc ctggcgagat agttggtgag cgcgccgacc
      721 ggccgcgaga tcgacatctc gttgtcgttc aggatcacca gcaggtcgac gtccatcgcg
      781 ccggcgttgt tcatcgcctc gaaggccatg ccggcggaca tcgcgccgtc gccgatcacc
      841 gcgatacagc gccttttttc attctttatt tttgcggaaa cggccattcc cagcgccgcc
      901 gagatggagg tgctcgaatg cgcagtgccg aaggtgtcgt acttcgactc ggcgcgccgc
      961 ggaaagcccg agatgccgtc gagcatgcgc aggcgcgaca tctgctcacg acggccagtc
     1021 aggatcttgt gcgcgtaggt ctggtggccg acgtcccaga cgatgcggtc ctcgggggtg
     1081 tcgaagacgt agtgcagcgc gatggtgagc tccaccgtgc cgaggttcga ggaaaggtgg
     1141 ccgccggtct tggacaccga ctcgagcacg tacgcgcgca gctcgtcggc gagctggccg
     1201 agctggcggc gctccagcgc gcgcagctgc gccggctcgt cgatcgtctt cagcagctcg
     1261 tacatcgacg gattgtagcc gtccgcattt tcctcagtgg gaagctctcc ggtgagggtg
     1321 ctcataccta gaattttctc agaacgatga aatccgccag ctcgcccagg cgccggccgc
     1381 ggccgccgag accctcgagc gcggcgtgcg cctccgcgcg cagctcctgc gccagctcgc
     1441 gcgcgcgcgg caggcccatc agggaaacgt aggtcggctt gccctgcttc gagtccttgc
     1501 ccgcggtctt gccgagcgtg gcggtgctgg cgtcgtagtc gagcacatcg tcgaccacct
     1561 ggaacgcgag gccgaccgcc ttggcgtatt tgtccagtct gtgcttttcg gcttcagccg
     1621 aagcctcgcc gcatgagaag cccagcagca ccgccgcgcg aatcagggcg ccggtcttgt
     1681 ggatgtgcat gaactcgagc tcgggcaggc tgagcgcctt gccggtcgcc tccaggtcga
     1741 tctgctggcc gccggccatg ccgcgcgagc ccgccgcggc gccgaggatg cgcaccatgt
     1801 cgagctggcg ctgcgggtcg tcggcgatct ggctctcggc aagcagctgg aacgccaggg
     1861 actgcagggc atcgccgacc aggagcgcgg tcgcctcgtc gtactcgacg tgcacggtgg
     1921 gcttgccgcg gcgcaggacg tcgtcgtcca tgcagggcag gtcgtcgtgc accagggaat
     1981 aggcgtggat catctcgacc gcggccgcgg cgatctccag ccgctcgctc ggcgcgccgc
     2041 tcacttcgcc cgccgcgaaa gcgagcagcg gccgcacgcg cttgccgcct tccagcgtgg
     2101 cgtagcgcat cgcctcgtgc aggcgcgcgg gcgcggcgtg cgccggcggc agcagcctgg
     2161 cgagcgccgc ctccatgcgg gcctgcacgt cgcccatcca ggcctggaaa tcggtctcag
     2221 ccgccctcag gttcatcgcg ctcgacggct gcggtgaggg gtttgagaac gtctccttcc
     2281 agaaccttca cctgctgctg cgcggactcg aggcgctgct ggcagaaacg ggcgagctcg
     2341 agtccgcgct tgtgcgcggc cagcgcctgt tcgagcgaca gatcgccgct ttccatgcgc
     2401 gaaacgattt tttccagctc ggccaaggct tgttcgaaac tgagcttggc ttcgcctgaa
     2461 ggcttgctca tcgtttggta agatactgtt tttctaggag cttcacaacg catggcggaa
     2521 ctcgggaccc tgcggcagat caagccgacc acgtgtcacc tcccggtgtc ctggtacttc
     2581 gatcccaaga tcttcgagct ggaacagaag cacctgttcg acgccggcgc gaattacgtc
     2641 ggccacgagc tgatggtgcc gaaggttggc gactatcaca cgctttcctg gacgggcgac
     2701 gcgaaggtgc tggtgcgcaa cgctgcaggt aaagagcagg gagtcgagct gctctccaac
     2761 gtctgccgcc accgccagtc gatcatgctg caaggccgcg gcaacgtgca gaacatcgtt
     2821 tgcccgctgc accgctggac ctacgatttg aagggcgagc tgctcggcgc gccgttcttc
     2881 gaggagacgc cgtgcgtgaa gctgcactcg acgccgctcg tcaactggaa cggcctgctc
     2941 ttcgccgggc cgcgcgaccc gcgcaaagac ctggcgcgca tcaccaccat ggccgactgg
     3001 gacttcagcg gctacgtcct cgagtcggtg cgcgtcgacg agtacgacat caactggaag
     3061 accttcgtcg agacctacct cgaggtctac cacgtcaatt tctaccatcc ggggctgggc
     3121 aactttaccg actgcgacaa cttcacgatc gactgcggcg aggagcactc cataca
//
LOCUS       DASYJA010000125         2254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1836373, whole genome shotgun sequence.
ACCESSION   DASYJA010000125 DASYJA010000000
VERSION     DASYJA010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1836373"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1118)
                     /locus_tag="VGO02_02665"
     CDS             complement(9..1118)
                     /locus_tag="VGO02_02665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV7476618.1"
                     /translation="MSVCFVAPLAWPVFSGDPAIKVVGGAEVQQAILARLLAANGFRV
                     SLVTLDYGQPDGVLIDGVTVHKAFTPDGGLPVLRFLHPRMSSMLRALRAADADVYYYR
                     STAMWAGVMAEFARRHGRCSIYAGASDRDFAPDTGGQVRYARDRWLFRHAMRTVDAIV
                     VQNLQQQEACRATYGRNSVYIPSCYQLPAASRPFQDDRVLWVGTIQPHKRPELLLELA
                     RRLPQRRFVMIGGPGAHPELYESIRRQAAALPNLEFKGFLPLAAVEPWFDRARVHVLT
                     SRFEGMPNVCLQAWARGVPTVASIDVGARLDGEPVYPHFEDLDKFASHIEGLFQEENS
                     WKEASGRCREYFERNHSPAEVLARYSSLLHGLVAA"
     gene            complement(1181..>2254)
                     /locus_tag="VGO02_02670"
     CDS             complement(1181..>2254)
                     /locus_tag="VGO02_02670"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV7476619.1"
                     /translation="WLYLVPIIIGGAIGAFHVDEIVPYFVEQLVINFHNAPGYLRDMV
                     MKPLFIPLFALLLGVAVARSEKAERYIVPIALSVWLIALLEIGFVAYSGVKLGVLASP
                     SMRGFFLAIGLHANDLGRLFAIAYALLLFTWWESKDPLLKTFLFVTMGVLAFALLLTF
                     SRGAFVGFLLINALFLAWKFNFKSASLAVGAFAICALLMPGYVYNRVLLGVGESADAV
                     SAGRIDGIWLPLLPETLKSPLWGNGLGSIMWSYPMQIDAMLTVGHPHNAFLEAVLDMG
                     LIGLGLLLAYYWHVWRGFRALGSNAFLSPELRGFFQGATAGLLALLITGMAGSSLRPE
                     SEAVYLWMAIGMMYGLQARRPAG"
BASE COUNT          403 a          754 c          808 g          289 t
ORIGIN      
        1 ggcggcggtc atgcggcgac cagcccgtgc agcaggctgg agtagcgcgc cagcacctcg
       61 gccggggagt ggttgcgctc gaagtattcg cggcaacgac cggaggcttc tttccaggaa
      121 ttttcctcct ggaagagacc ctcgatatga gaggcaaact tatccaggtc ttcgaaatgc
      181 ggatacaccg gctcgccgtc caggcgcgcg ccgacgtcga tgctcgccac cgtgggcacg
      241 ccgcgcgccc aggcctgcag gcacacgttg ggcatgccct cgaagcgcga ggtcaggacg
      301 tgcacgcggg cgcggtcgaa ccagggctcg accgcggcga gcggcaggaa gcccttgaac
      361 tccaggttcg gcagggcggc cgcctggcgg cggatcgact cgtagagctc ggggtgcgcg
      421 cccgggccgc cgatcatgac gaagcgccgc tgcggcagcc ggcgcgccag ctcgagcagc
      481 agctcggggc gcttgtgcgg ctggatcgtg ccgacccaca gcacccggtc gtcctggaaa
      541 ggacgcgacg cggcgggcag ctggtagcag ctcgggatgt agaccgagtt gcgcccgtag
      601 gtggcgcggc aggcttcctg ctgctgcagg ttctgcacga cgatggcgtc gacggtgcgc
      661 atcgcatggc ggaacagcca gcggtcgcgc gcgtagcgga cctgcccgcc ggtgtccggc
      721 gcgaagtcgc ggtccgaggc gccggcgtag atcgagcatc ggccgtggcg gcgggcgaat
      781 tcggccatca ccccggccca catcgcagtc gagcggtagt agtagacatc ggcgtccgcg
      841 gcgcgcagcg cgcgcagcat cgagctcatg cgcggatgca ggaagcgcag caccggcagg
      901 ccgccgtctg gcgtgaaggc cttgtgcacg gtgacgccgt cgatcagcac gccgtcgggc
      961 tggccgtagt cgagcgtcac cagggagacg cgaaagccgt tcgccgccag caggcgcgcg
     1021 aggatcgcct gctgcacctc ggcgccgccg accaccttga tggccgggtc gcccgagaac
     1081 accggccagg cgagcggcgc cacgaagcag accgacaacg ctctctccgg gcggttcgcg
     1141 ggggccgcgg cgccctgacg cacactctct tcgatgaaca tcagcccgcc ggccgccgcg
     1201 cctgcaggcc gtacatcatg ccgatcgcca tccacaggta gaccgcctcg gattccggcc
     1261 gcaggctgct gcccgccatg ccggtgatca ggagcgccag cagtcccgcg gtggcaccct
     1321 ggaagaagcc gcgcagctcg gggctgagga aggcgttgct gcccagcgcg cggaagccgc
     1381 gccagacgtg ccagtagtag gcgagcagca gcccgagccc gatcaggccc atgtcgagca
     1441 ccgcctcgag gaaggcgttg tgcggatggc cgaccgtcag catggcgtcg atctgcatcg
     1501 ggtaggacca catgatcgag cccaggccgt tgccccacag cgggctcttc agcgtctccg
     1561 gcaggagcgg cagccagatg ccgtcgatgc gcccggcgct caccgcgtcg gcgctctcgc
     1621 cgacgccgag cagcacccgg ttgtagacgt agcccggcat gagcagcgcg cagatcgcga
     1681 acgccccgac ggcgagcgac gccgacttga agttgaactt ccaggcgagg aacagcgcgt
     1741 tgatcagcag gaagccgacg aaggcgccgc gcgagaaagt gagcagcagg gcgaaggcga
     1801 gcacccccat ggtgacgaac aggaaggtct tcagcagcgg gtccttcgac tcccaccagg
     1861 tgaacagcag cagggcgtag gcgatcgcga acagccggcc gaggtcgttg gcgtgcaggc
     1921 cgatggcgag gaagaagccg cgcatcgacg ggctggccag cacgccgagc ttgacccccg
     1981 agtaggcgac gaagccgatc tcgagcagcg cgatgagcca caccgacagc gcgatcggca
     2041 cgatgtagcg ctcggccttc tccgagcggg caaccgccac gccgagcagc agcgcgaaga
     2101 gggggatgaa gagcggcttc atcaccatgt cgcgcaggta gccgggcgcg ttgtggaagt
     2161 tgatcaccag ctgttccacg aagtacggca cgatctcgtc gacgtggaag gcgccgatcg
     2221 ccccgccgat gatgatcggc accaggtaca gcca
//
LOCUS       DASYJA010000126         2871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_747295, whole genome shotgun sequence.
ACCESSION   DASYJA010000126 DASYJA010000000
VERSION     DASYJA010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747295"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VGO02_02675"
     CDS             <1..195
                     /locus_tag="VGO02_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476620.1"
                     /translation="DPLEGQTFRVGKLSLGYIHDLPTDGHFKVGFGGLVSRYSLPGDL
                     EPAYGGSPTSFMLFARIKIQ"
     gene            232..1356
                     /locus_tag="VGO02_02680"
     CDS             232..1356
                     /locus_tag="VGO02_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEV7476621.1"
                     /translation="MASAHPHPVRSAHAAGLRYVSDNAPGIARRRTASGFRYLNGGGR
                     AVRDAATLARIRRLAIPPAWERVWICPREDGHLQATGRDARGRKQYRYHPQWREIRDE
                     TKYGRMAAFGRALPRIRRRVRKDLALAGLPREKILATLVRLLETTFIRVGNDEYAREN
                     SSFGLTTLRNRQVQVDGAKIRFQFRGKSGVEHAIELHDRRMAGIVRRVRDLPGQELFQ
                     YLDENGERRAIESADVNAYIREAARGEFTSKDFRTWAGTLLTARALREAGAFESKAHA
                     MRNVATAIESVARQLGNTKAVCRKCYVHPAVVDAYLEGRLQDGAAPGEAAVIALLRRK
                     KPRRDSLAPLLRRSISRSRLSRRGRGLAVHPALVPGQGQP"
     gene            complement(1312..1794)
                     /locus_tag="VGO02_02685"
     CDS             complement(1312..1794)
                     /locus_tag="VGO02_02685"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HEV7476622.1"
                     /translation="MDIAKPDGAGRTEQIKLDSAAGHLLEECRMVLPGIQALFGFQLI
                     AVFNQGFGEKLSGPQQQLHFAALFLVAVAAGLVMAPAAIHRQTQQREVSERFIWLSSW
                     LLMLSMFPLAAGLSLDLYLIGMIVFEAPGISIAFAIILFAVLMALWLALPRHERRMHS
                     "
     gene            complement(1798..2205)
                     /locus_tag="VGO02_02690"
     CDS             complement(1798..2205)
                     /locus_tag="VGO02_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HEV7476623.1"
                     /translation="MKKVLFICIKNTSRSPMAEALAAMLAPGWEVYSAGMHPGKEPNP
                     EAVRAMKELGCDISKHRPKHVSDFQKITFDLVAKMDAPDVSDFVTAKWIENWDVPDPA
                     NGGIEEFRKVRDMLAERVRKLAGGEQKGLSKAA"
     gene            complement(2210..2803)
                     /locus_tag="VGO02_02695"
     CDS             complement(2210..2803)
                     /locus_tag="VGO02_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HEV7476624.1"
                     /translation="METKQLKELLLQSLEHERGGVKVYEAALKCVQNEDLQEEFEKYH
                     QETEHHVEVLQDVFAQMQLDPEEESPGRKIIRDMGQALVAAMEAALGAGDPAAAEIVA
                     CECVTIAELKDHLDWELIGEAAKKMSGAEGKALKEAYAEIEDQEDEHFYHSRGWTREL
                     WMDALGLKAVLPPPEERKHVKTAIGAARAEQERKNMR"
BASE COUNT          440 a         1010 c          931 g          490 t
ORIGIN      
        1 gacccgctcg agggccagac tttccgcgtc ggcaagctca gcctcggcta catccacgac
       61 ctgccgaccg acggccactt caaggtcggc ttcggcggcc tcgtcagcag gtattccctg
      121 cccggcgacc tcgagcccgc ctatggcggc agcccgacct ccttcatgct ctttgcgcgc
      181 atcaagatcc aatgagactc gtgcggcacg ccatttgcac cggtgcgtgc catggccagc
      241 gcccaccccc acccggtccg ttcggcccac gccgccggcc tgcgctacgt ctcggacaac
      301 gctccgggaa tcgctcgccg caggacggcg agcggctttc ggtacctgaa cggcggcggc
      361 cgggcggtgc gcgatgccgc aacgctggcg cgcattcggc gcctggcgat tccgccggcg
      421 tgggaaaggg tctggatctg cccgcgcgag gacgggcacc tgcaggcgac cgggcgcgat
      481 gcgcgcgggc gcaaacaata ccgctaccac ccgcagtggc gcgagatccg cgacgagacc
      541 aagtacggcc ggatggcggc cttcggccgc gcgctgccgc gcatccgccg ccgcgtgcgc
      601 aaggacctcg ccctggcggg attgccgcgc gagaagatcc tggcgacgct ggtgcgcctg
      661 ctcgagacaa cgttcatccg cgtcggcaac gacgaatatg cgcgcgagaa ttcatccttc
      721 ggactgacca cgctgcgcaa ccgccaggtg caggtcgacg gcgcgaagat ccgcttccag
      781 ttccgcggca agagcggcgt cgagcacgcc atcgagctgc acgaccggcg catggccggc
      841 atcgtgcggc gcgtgcgcga cctgccgggc caggagctgt tccagtacct cgacgagaat
      901 ggcgagaggc gcgcgatcga gtcggcggac gtcaacgcct acatccgcga ggcggccagg
      961 ggcgagttca ccagcaagga tttccgcacc tgggccggca ccctgctgac cgccagggcg
     1021 ctgcgcgagg ccggcgcctt cgaatcgaag gcgcacgcca tgcgcaatgt cgccaccgcc
     1081 atcgaatcgg tggcgcgcca gctcggcaac accaaggcgg tgtgccgcaa atgctacgtc
     1141 cacccggcgg tcgtcgacgc ctacctcgag ggccggctgc aggacggcgc agcgcccggc
     1201 gaggccgcgg tcatcgcgct actgcgcagg aagaagccgc ggcgcgattc gctcgcgccg
     1261 ctcttgcgcc gctccatttc gaggtcgcgc ctttcgcgcc gaggtcgggg gctagctgtg
     1321 catcctgcgc tcgtgcctgg gcagggccag ccatagcgcc atcagcacgg cgaacaggat
     1381 gatggcaaat gcgatgctga tgcccggggc ctcgaacacg atcatgccta tcaggtagag
     1441 gtccaggctc agaccggcgg caagcgggaa catgctgagc atcagcagcc aggacgaaag
     1501 ccagatgaac cgctcgctga cctcacgctg ctgcgtctgg cggtggatcg ctgccggcgc
     1561 catgaccagc ccggccgcga cggcgacgag gaacagcgcc gcgaagtgga gctgctgctg
     1621 cgggccggac agcttctcgc cgaagccctg gttgaacacg gcaatcagct ggaagccgaa
     1681 cagcgcctgg atcccgggca gcaccatgcg gcattcctcg agcaggtggc ccgcggccga
     1741 atccagcttg atctgctcgg tccgcccggc gccgtcgggc ttggcgatgt ccatcgctca
     1801 ggccgccttc gacagcccct tctgttcgcc gcccgcgagc ttgcgcacgc gctcggcgag
     1861 catgtcgcgc accttgcgga actcctcgat gccgccgttc gccgggtctg gaacgtccca
     1921 gttctcgatc catttcgcgg tgacgaaatc gctcacgtcg ggcgcgtcca tcttggcgac
     1981 gaggtcgaag gttattttct ggaagtcgga gacgtgcttc ggccggtgct tgctgatgtc
     2041 gcagccgagc tccttcatcg cgcggaccgc ctccgggttg ggctcctttc ccggatgcat
     2101 gcccgcgctg tagacctccc agccgggcgc cagcatggcg gcgagcgcct ccgccatggg
     2161 gctgcggctg gtgttcttga tgcagatgaa cagtactttt ttcatccgat cagcgcatgt
     2221 tcttgcgctc ctgctcggcg cgcgcggcac cgattgcggt cttgacgtgc ttgcgctcct
     2281 cgggcggcgg cagcacagcc ttcaggccaa gtgcgtccat ccaaagctcg cgggtccagc
     2341 cgcgcgaatg gtagaagtgc tcgtcttcct ggtcctcgat ctcggcatac gcctccttca
     2401 gcgccttgcc ttcggcgccc gacatcttct tcgccgcctc gccgatcagc tcccagtcga
     2461 ggtggtcctt cagctcggcg atggtcacgc actcgcaggc gacgatctcg gccgccgcgg
     2521 ggtcgccggc gccgagcgcc gcttccatcg cggcgacgag ggcctggccc atgtcgcgga
     2581 tgatcttgcg gcccgggctt tcctcctccg ggtcgagctg catctgcgcg aacacgtcct
     2641 gcagcacctc gacgtggtgc tcggtctcct gatgatattt ctcgaattct tcctgcaggt
     2701 cctcgttctg cacgcatttc agcgccgcct cgtagacctt cacgccgccg cgctcgtgct
     2761 cgagcgactg cagcagcaac tccttcaatt gtttggtttc catcgatcct ccgggttgga
     2821 acgccggaag agcagcaata ttcgttccga gcggcttcag gctagcgtca g
//
LOCUS       DASYJA010000127         3282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3017129, whole genome shotgun sequence.
ACCESSION   DASYJA010000127 DASYJA010000000
VERSION     DASYJA010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3017129"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1847)
                     /locus_tag="VGO02_02700"
     CDS             complement(<1..1847)
                     /locus_tag="VGO02_02700"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEV7476625.1"
                     /translation="MDAPQPGNLAPDLRPGLKRKLAWRWNRLRCMSPLEVAHRVLRAA
                     QARAERSGWLRTPEAPRADLAAPAQPWVHADANVDAARYRAAAERIADGWLDVFALRN
                     VDIGSPPRWNRDPKTGIEAPLEFGKLLDYRDSDLVGDIKYLWEPNRHLHLVTLAQAFS
                     LTGERKYLDALAEHLDSWFLACPYGCGPNWSSALEVAIRLANWSAAWQLIGFSSLDTE
                     LRDRWLKSIYEHAEFVSGWLSRHSSANNHLIGEATGLYLAALTWPYWPRARAWRAQAT
                     AILEREALAQHTPDGVNREQAVAYQQFVLEMLLACLLAGRANGQRFSSAYEARIEAMM
                     DFLASIMDAGGNLPMFGDADDGVLLGLEPGGFAACRPLLAAGAILFRRGDFKLKARTL
                     DDRTRWLLGRDAERLFEALDGEKTRLPLRQTFPEGGYFILGCEFDGASEIRLVADAGP
                     LGYRSIAAHGHADALSFTLSAGGRELLVDPGTYAYHTHPAWRHYFRGTGAHNTVRIDR
                     FDQSVPGGNFMWLAKARAGCSLWLSSAEKDSFEGWHDGYMRLDDPVKHRRLIVLDKRS
                     RRVVLEDTLEMEDEHDVELLFHCSEGCAVDAVPGGYLLSQKSASVKMLLP"
     gene            complement(1796..>3282)
                     /gene="asnB"
                     /locus_tag="VGO02_02705"
     CDS             complement(1796..>3282)
                     /gene="asnB"
                     /locus_tag="VGO02_02705"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEV7476626.1"
                     /translation="LAFDGSIDNRDELRKALQVRSYEFRTGSDDELVLRAYQHWDKDV
                     VTRLRGRFALALWDARKERLLLARDRFGEKPLYLADKDGVLYFASEPKALRAILPLVV
                     DLEAVRDCLVHSYVPGPRTMFAGVRKLAPASLALWQFGKLRETRYWSLPDAQTIPGKA
                     PADAVQGFLVRLDEAVALQAGEGCGVLLSGGLDSAVLLALASHRHPKIATFSLGCAGD
                     KRSELPQAAQVAKHFATRHHELDAQPKDLVDGLPRLIALRDAPLALPSDLALHALLHE
                     VAKSCKAVLGGDGSDEMLGGYRRYVAAKFWPNAALRSREENAILLAEDFPRAKRPAEK
                     RPQGSTDLRRLLHTDQSAWLSDNLLERDERIAAAASLEVRLPFLDHRLAEYVSSLPDE
                     ERVHGLSTKWILRRAARRVLPPRLPQRRKLGWPGRVKEWMRGELRESLGDHLQGTNAM
                     TRRYYRGETLDRAIDEHLNGKKDHGTLLWTLLNLEIWHRTYARG"
BASE COUNT          484 a         1133 c         1170 g          495 t
ORIGIN      
        1 ggaagaagca ttttcactga agcggatttt tgcgaaagca aatagccccc cggaaccgca
       61 tccaccgcgc aaccctcgct gcagtggaag agcagctcca cgtcgtgctc gtcctccatc
      121 tccagcgtgt cctcgagcac cacgcggcgg ctgcgcttgt cgagcacgat caggcggcgg
      181 tgcttcaccg ggtcgtccag gcgcatgtag ccgtcgtgcc agccctcgaa gctgtccttt
      241 tcggccgagg acagccacag gctgcagccg gcgcgcgcct tggcgagcca catgaagttg
      301 ccgccgggca ccgactggtc gaaccggtcg atgcgcacgg tgttgtgcgc gccggtgccg
      361 cggaagtagt gccgccacgc cggatgggtg tgataggcgt aggtgcccgg atcgaccagc
      421 agctcgcgcc cgccggccga gagcgtgaac gacagcgcgt cggcatggcc gtgcgcggcg
      481 atcgagcggt agccgagcgg gccggcgtcg gccaccaggc ggatctcgct ggcgccatcg
      541 aattcgcagc cgaggatgaa gtagccgcct tcggggaagg tctggcgcag cggcagccgg
      601 gtcttttcgc cgtcgagcgc ctcgaacagg cgctcggcat cgcggccaag cagccagcgc
      661 gtgcggtcgt cgagcgtgcg cgccttcagc ttgaagtcgc cgcgccggaa caggatcgcg
      721 ccggcggcga gcagcgggcg gcaggcggcg aagccgcccg gctccaggcc gagcagcacg
      781 ccgtcgtcgg cgtcgccgaa catcggcagg ttgccgcccg cgtccatgat cgaggcgagg
      841 aagtccatca tcgcttcgat ccgcgcctcg taggcgctcg agaagcgctg gccgttcgcc
      901 cggccggcga gcaggcaggc gagcagcatt tcgagcacga actgctggta ggccaccgcc
      961 tgctcgcggt tgacgccgtc gggcgtgtgc tgcgccagcg cctcgcgctc gaggatcgcc
     1021 gttgcctgcg cccgccaggc gcgcgcgcgc ggccagtacg gccaggtgag cgcggcgagg
     1081 tacaggccgg ttgcttcgcc gatgaggtgg ttgttggccg aggaatgccg cgacagccag
     1141 cccgagacga attccgcgtg ctcgtagatg gatttgagcc atcggtcgcg caactcggtg
     1201 tcgaggctcg aaaaaccgat caactgccat gcagccgacc agttcgcgag gcggattgcc
     1261 acctccagcg cgctcgacca gttcggcccg cagccatacg ggcaggcgag gaaccagctg
     1321 tcgaggtgct cggcgagcgc gtccaggtac ttgcgctccc cggtgagcga gaaggcctgc
     1381 gccagcgtga ccaggtgcag gtgccgattg ggctcccaca ggtacttgat gtcgccgacc
     1441 aggtcggaat cgcggtagtc gagcagcttg ccgaattcca gcggcgcctc gatgccggtc
     1501 ttcgggtcgc ggttccagcg cggcggcgag ccgatgtcga cgttgcgcag ggcaaagacg
     1561 tcgagccagc cgtcggcgat gcgctcggcg gccgcgcggt agcgcgccgc gtcgacgttg
     1621 gcgtcggcgt gcacccaggg ctgcgccggc gcggcgaggt ccgcccgcgg cgcctcgggc
     1681 gtgcgcagcc agcccgagcg ctcggcgcga gcctgcgccg ctcgcaggac gcggtgcgcc
     1741 acctccagcg gcgacatgca gcgcaggcgg ttccagcgcc aggcgagctt gcgcttcagc
     1801 cccgggcgta ggtccggtgc cagatttcca ggttgaggag cgtccacagc agcgttccgt
     1861 gatctttctt tccgttgagg tgctcgtcta tcgctcggtc cagcgtctcg ccgcggtaat
     1921 agcggcgcgt catggcattc gttccctgca ggtggtcgcc gagcgactcg cgcagctcgc
     1981 cgcgcatcca ttccttgacg cgccccggcc agccgagctt gcgccgctgc ggcaggcgcg
     2041 gcggcagcac gcggcgcgcc gcgcggcgca ggatccattt cgtcgacagg ccgtgcacgc
     2101 gctcctcgtc gggcagcgac gagacgtatt cggcgaggcg gtggtcgagg aacggcaggc
     2161 gcacctcgag cgacgccgca gcggcgatgc gctcgtcgcg ctcgagcagg ttgtccgaga
     2221 gccaggcgct ctggtcggtg tgcagcaggc gcctcagatc agtgctgccc tgcggccgct
     2281 tctcggcggg tctttttgct cttggaaaat cttccgccaa caagatcgcg ttctcttcac
     2341 gggagcgcag ggccgcattg ggccagaact tcgccgccac gtaccggcgg taccctccca
     2401 gcatctcgtc gctgccgtca ccgccgagga ctgctttgca ggacttggct acctcgtgca
     2461 acagcgcatg cagcgccagg tcggagggca gcgcgagggg cgcgtcgcgc agcgcgatca
     2521 ggcgcggcag cccatcgacc aggtccttgg gctgcgcgtc gagctcgtgg tgccgggtgg
     2581 cgaagtgctt cgccacctgc gccgcctgcg gcagctcgct gcgcttgtcg cccgcgcagc
     2641 cgagcgagaa ggtggcgatc ttcggatggc ggtggctggc cagcgcgagc agcacggccg
     2701 agtcgagacc gcccgagagc agcacgccgc agccctcgcc cgcctgcagc gcgacggcct
     2761 cgtcgagccg cacgaggaat ccctgcaccg cgtccgccgg cgccttgccg gggatggtct
     2821 gcgcgtcggg caggctccag tagcgcgtct cgcgcagctt gccgaactgc cacagcgcga
     2881 ggctcgccgg ggcgagcttg cgcacgccgg cgaacatggt gcgcgggccg ggcacgtagc
     2941 tgtgcaccag gcagtcgcgc accgcctcga ggtccacgac gagcggcagg atcgcgcgca
     3001 gcgccttggg ctccgaggcg aagtacagca ccccgtcctt gtccgcgagg tagagcggct
     3061 tctcgccgaa gcggtcgcgc gcgagcagca gccgctcctt gcgcgcatcc caaagcgcca
     3121 gcgcgaagcg cccgcgcagg cgcgtcacca cgtccttgtc ccagtgctgg taagcccgca
     3181 gcaccagctc atcgtcgctg ccagttctga attcatagga acgcacctgc agcgccttgc
     3241 gcagctcgtc gcggttgtcg atcgagccgt cgaaggcgag cg
//
LOCUS       DASYJA010000128         4725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3108008, whole genome shotgun sequence.
ACCESSION   DASYJA010000128 DASYJA010000000
VERSION     DASYJA010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3108008"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VGO02_02710"
     CDS             <1..311
                     /locus_tag="VGO02_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476627.1"
                     /translation="TAYEVRRFPKPLNPGHRPFDGGKDQTMTNATVGTVAQSSDGREL
                     TLTYDGGSQKISVPQDTSVSDLVPGKPAQLVPGARVNLTHGPDKVALRIQVSAPKPPQ
                     "
     gene            complement(295..1032)
                     /locus_tag="VGO02_02715"
     CDS             complement(295..1032)
                     /locus_tag="VGO02_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7476628.1"
                     /translation="MATDILTLEGVVAGYGNLTVLNETTLGAPRGAITTIIGPNGAGK
                     STVFKTVFGLLPVRAGRILFEGREVTNLSPRKLLELGISYVPQGRNIFPELSVRHNLE
                     LGATAAAAGVDVAARIESVMDRFPMLRQRAAAQASTLSGGQQKLLEIARSLLLDPRLM
                     LIDEPSIGLSPRLVQETFAILQDLRAKGMSILMVEQNAKRALEISDFGVVIELGRTRM
                     TGEARAILADPKVGQLFLGGGLSTEAA"
     gene            complement(1022..1765)
                     /locus_tag="VGO02_02720"
     CDS             complement(1022..1765)
                     /locus_tag="VGO02_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7476629.1"
                     /translation="MNAVLEVHDLAKSFGGIRAVDGVSFDVREGEILGLIGPNGSGKS
                     TLFNCILGQLPPSAGEVRIDGRAITGMRPCELNRLGVGRTFQLLQVFPQLTVRENLIL
                     AGQEHQSSMWSRLVGRRDAGLGPAADRMIEFFRLAALAAENAGALSYGQQKLLDVAMA
                     FMAGPRLVLLDEPAGGVNPTMLAGLRERLREINAAQRATFVVIEHNMEFVMSLCTRIL
                     VLDQGRIIAEGAPAAVRSDPKVIEAYLGH"
     gene            complement(1762..2685)
                     /locus_tag="VGO02_02725"
     CDS             complement(1762..2685)
                     /locus_tag="VGO02_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV7476630.1"
                     /translation="MKKLFVLLLLVVLIAAPFFLKPYGIYLLSLWVVMTIAAIGLNLT
                     LGYAGQISLAQGSFVGIGAYAAALLTTAGWPLWSAFLLAIALSLAIGWVLGYPALRVQ
                     HHYLAFVTLAFATLVFLMLRNEEWLTKGVYGIPGIPRPVLFGWPTQAAIRYYFFCLGV
                     LGLVSLATWWLVRSPWGRAFTALRENAIRADSLGVDTRRYTLMAFAMGSALGGIAGVL
                     YAPLVQFIEPNAFNLNLSLNLLLMVMVGGSGYFLGPFLGAAVSVLLPEWMRFGSEGLY
                     LIFYAIAVMVLMAFCPSGLLGLVERARRAIR"
     gene            complement(2682..3560)
                     /locus_tag="VGO02_02730"
     CDS             complement(2682..3560)
                     /locus_tag="VGO02_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV7476631.1"
                     /translation="MADFLQLAVAGIATGGIYALVAVGFVLLWQTSQTINFAQGEFVM
                     LPAFFVLAAMKFGGLSFPAALLVGLLAALLVLGLLFKRLVVDPILRHGVLPLVISTLG
                     LAILFKEGVKELYSAEAQGFPGVWPTTNLSIGGAVISAQSLVIIGVALAAVALLQYFL
                     GATRTGRQMQATAQNPQLARVLGIPVERMILYTFLINAALVALASFLVSPIYLAKFTN
                     GELLGLAAFVAAIVGGFNQVRGAIVGGLLLGVIDNFVAAYWSSQYRAAIPLFLLIAVI
                     LWRPQGLLGRAEERTV"
     gene            complement(3563..4678)
                     /locus_tag="VGO02_02735"
     CDS             complement(3563..4678)
                     /locus_tag="VGO02_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7476632.1"
                     /translation="MGALAASIAFCAQAQQTVFMPIVIEVSGNGAVSGTNFRDGALMA
                     IDEINAKGGILKRRINAPVSDTQSNAGISRAQVQKAIDGNPYVILGPVFSGSVKVNMI
                     LAQQAEIPQMTGAEAAEITQMGNDYIFRTSFGQQMSMPKMAIFVTQSLKAKTVAFLWV
                     NNDYGKGGRDVFFKEMAARSIKIVADVSTESGQVDFAADIVKLKAANADAIFIYTNEE
                     ECARVLREARKQGVKTPIIGETNVVSQKVIELAGDTANGVKGHVGLTADAPIPAVLEF
                     RDKFQKRFKYLPDHNGIKGYTAVYAVKHATEKLGKFDRKALAKLMHGLTITPQQEPGI
                     LIETTWDAKGDIDRISFLVEVVDGKQKVVEIMPKLGK"
BASE COUNT          827 a         1590 c         1582 g          726 t
ORIGIN      
        1 tgacggccta cgaagtccgg cgcttcccca agccgctcaa tccggggcac cggccgttcg
       61 acggtggcaa ggatcagacg atgaccaacg ccaccgtggg cacggtggcg cagtcatccg
      121 acggccgcga gctgaccctc acctacgacg gcggctcgca gaagatcagc gtgccgcagg
      181 acacgtcggt atcggacctg gtgccgggca agccggcgca gctggtcccc ggcgcgcggg
      241 tcaatctcac gcacggcccc gacaaggtgg ccttgcgtat ccaggtttca gcgcctaagc
      301 cgcctcagta gacaacccgc cgccgaggaa cagctggccg accttgggat cggcgaggat
      361 ggcgcgcgcc tcgcccgtca tgcgcgtgcg cccgagctcg atgaccacgc cgaaatccga
      421 gatctcgagc gcgcgcttgg cgttctgctc gaccatcagg atcgacatgc ccttcgcgcg
      481 caggtcctgc aggatcgcga aggtctcctg caccagccgc ggcgaaagcc cgatcgaggg
      541 ctcgtcgatc agcatcagcc tggggtcgag cagcaggctg cgcgcgatct cgagcagctt
      601 ctgctgcccg ccggagagcg tcgacgcctg cgcggcggcg cgctgccgca gcatcggaaa
      661 gcggtccatc accgactcga tgcgcgccgc gacgtcgacg cccgccgccg ctgcggtcgc
      721 gccgagctcg aggttgtggc gcacgctcag ctcggggaat atgttgcgac cctgcggcac
      781 gtaggaaatg cccagctcga gcagcttgcg cgggctcagg ttcgttacct cgcgcccctc
      841 gaacaggatg cgtcccgccc gcaccggcag gagcccgaac acggtcttga acacggtcga
      901 cttgcccgcg ccgttgggcc cgatgatggt cgtgatcgcg ccgcgcggcg cgcccaaggt
      961 cgtctcgttc agcaccgtta ggttgccgta gccggcgacg acgccctcca gggtcagaat
     1021 atcagtggcc aagatacgct tcgatcacct tgggatcgct gcgcaccgcc gccggggcgc
     1081 cctcggcgat gatgcggccc tggtccagca cgaggatccg cgtgcacagg gacatgacga
     1141 attccatgtt gtgctcgatg acgacgaagg tcgcgcgctg cgcggcgttg atctcgcgca
     1201 ggcgctcgcg cagcccggcg agcatggtcg ggttgacgcc gccggcgggc tcgtcgagca
     1261 gcaccagccg cggcccggcc atgaacgcca tcgccacgtc gagcagcttc tgctggccgt
     1321 agctcagcgc gccggcgttc tccgcggcga gcgcggccag gcggaagaac tcgatcatgc
     1381 ggtcggccgc cgggccgagg ccggcgtcgc gccggccgac cagccgcgac cacatcgagc
     1441 tctggtgctc ctgcccggcg agaatcaggt tctcgcgcac ggtcaactgg gggaagacct
     1501 gcagcagctg gaaggtgcgg ccgacgccga gccggttgag ctcgcacggc cgcatgccgg
     1561 tgatcgcgcg cccgtcgatg cgcacctccc cggccgaggg cggcagctgg ccgagaatgc
     1621 agttgaacag cgtcgacttg cccgagccgt tcggaccgat caagccgagg atctcgcctt
     1681 cgcgcacgtc gaaggagacg ccgtccacgg cgcggatgcc gccgaacgac ttcgccaggt
     1741 cgtgtacctc gaggaccgcg ttcaccggat ggcccttctt gcgcgttcta cgagtccaag
     1801 cagccctgag gggcagaaag ccatgagcac catcaccgcg atggcgtaaa agatgaggta
     1861 caggccctcg ctgccgaagc gcatccattc gggcagcagc accgagaccg cggcgccgag
     1921 gaacgggccg aggaagtagc ccgagccgcc gaccatcacc atcagcagaa ggttcaggga
     1981 caggttgagg ttgaacgcgt tcggttcaat gaactggacc agcggcgcgt acagcacgcc
     2041 cgcgattccg cccagcgcgg atcccatggc gaacgccatc agcgtgtagc gccgggtgtc
     2101 gacgccgagc gagtcggcgc ggatcgcgtt ctcgcgcagc gccgtgaacg cgcgtcccca
     2161 cggcgagcgc accagccacc acgtcgccag tgaaaccagc ccgagcacgc ccaggcagaa
     2221 gaagtaatac ctaatagccg cctgggtcgg ccagccgaac agcaccggcc gcggaatgcc
     2281 ggggatgccg tagacgcctt tggtgagcca ttcttcgttg cgcagcatca ggaagacgag
     2341 ggtcgcgaac gcaagggtga cgaaggcgag gtagtggtgc tgcacgcgca acgccgggta
     2401 accgagcacc cagccgatcg ccaggctgag cgcgatcgcc agcaggaaag ccgaccacaa
     2461 cggccagccc gccgtggtga gcagcgccgc ggcgtaggcg ccgatgccga cgaacgagcc
     2521 ttgcgcgagc gagatctggc ccgcgtagcc gagggtgagg ttcaggccga tcgcggcgat
     2581 ggtcatcacg acccagaggc tgagcaggta gatcccgtac ggcttcagga aaaacggcgc
     2641 cgcgatcagc acgaccagaa gcagcagcac aaagagcttc ttcataccgt ccgctcttct
     2701 gcgcggccga gcagcccctg cggccgccac aggatcaccg cgatcagcag gaacagcggg
     2761 atcgcggcgc ggtactgcga cgaccagtag gcggcgacga agttgtcgat gacgccgagc
     2821 agcaggccgc cgacgatcgc gccgcgcacc tggttgaagc cgccgactat agcggcgaca
     2881 aacgcagcga gccccagtag ttcaccattt gtgaacttgg cgaggtagat cgggctgacc
     2941 aggaaggagg cgagcgccac gagcgcggcg ttgatcagga acgtatacaa aatcatgcgc
     3001 tcgaccggga tgccgagcac gcgggcgagc tgcgggttct gcgcggtggc ctgcatctgc
     3061 cggccggtgc gcgtggcgcc gaggaaatac tgcagcaggg cgacggcggc gagcgccacc
     3121 ccgatgatca ccaggctctg cgcggagatg accgcgccgc cgatcgagag gttggtggtc
     3181 ggccatacgc ccgggaaccc ctgcgcctcg gcggagtaga gctccttgac cccctccttg
     3241 aagaggatgg cgaggcccag ggtcgagatg acgagcggca ggacgccgtg gcgcaggatc
     3301 gggtcgacca ccaggcgctt gaaaagcaac ccgagcacga ggagggcggc aagcaggccg
     3361 accagcagcg ctgccgggaa ggagaggccg ccgaacttca tcgccgccag cacgaagaag
     3421 gccggcagca tgacgaactc gccctgcgcg aagttgatgg tctgcgaggt ctgccacagc
     3481 agcacgaagc cgaccgcgac gagcgcgtag atgccgcccg tcgcgatgcc cgcgaccgcg
     3541 agctgcagga agtcggccag gactacttgc ccagcttggg catgatctcg acgaccttct
     3601 gcttgccgtc gactacctcg accaggaagc tgatgcggtc gatgtcgccc ttcgcgtccc
     3661 aggtggtctc gatcaggatg cccggctcct gctgcggcgt gatggtcagg ccgtgcatca
     3721 gcttggcgag cgccttgcga tcgaacttgc ccagcttctc ggtcgcgtgc ttgaccgcgt
     3781 agaccgcagt gtagcccttg atgccgttgt ggtcgggcag gtacttgaag cgcttctgga
     3841 acttgtcgcg gaactcgagc accgccggga tcggcgcgtc ggcggtcagg ccgacgtgcc
     3901 ctttcacgcc gttggccgtg tcgccggcga gctcgatcac cttctggctg acgacgttgg
     3961 tctcgccgat gatcggcgtc ttcacgccct gcttgcgcgc ctcgcgcagc acgcgcgcgc
     4021 actcctcctc gttggtgtag atgaagatgg cgtcggcgtt cgccgccttc agcttgacga
     4081 tgtcggcggc gaagtccacc tggccggact cggtcgacac gtcggcgacg atcttgatgc
     4141 tgcgcgccgc catctccttg aaaaacacgt cgcgtccgcc cttgccatag tcgttgttca
     4201 cccacaggaa ggccaccgtc ttcgccttga ggctctgggt gacgaagatc gccatcttcg
     4261 gcatgctcat ctgctgcccg aacgaggtgc ggaagatgta gtcgttgccc atctgcgtga
     4321 tctcggcggc ttccgcgccg gtcatctgcg ggatctcggc ctgctgggca aggatcatgt
     4381 tcaccttcac cgagccggag aacaccgggc cgaggatcac gtacgggttg ccgtcgatcg
     4441 ccttctgtac ctgggcgcgc gagatgccgg cgttgctctg cgtatcgctc accggcgcgt
     4501 tgatcctgcg cttgaggatg ccgcccttgg cgttgatctc gtcgatcgcc atcagcgcgc
     4561 cgtcgcggaa gttggtgccc gacaccgcgc cgttgcccga tacctcgatc acgatcggca
     4621 tgaacaccgt ctgctgcgcc tgcgcgcaga acgcgatgct tgctgcgagt gcccccatca
     4681 gtgctttcac gtcccccctc ctctctagag tccctcgtac tgcag
//
LOCUS       DASYJA010000129         6807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2836195, whole genome shotgun sequence.
ACCESSION   DASYJA010000129 DASYJA010000000
VERSION     DASYJA010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6807
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2836195"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VGO02_02740"
     CDS             complement(<1..726)
                     /locus_tag="VGO02_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV7476633.1"
                     /translation="MCGKHRTDTEHAAALAEERIVEEALLRALLPDSALRRRFLQAVG
                     AGTALAALSTVFPLGAAKEAFAQGAGKPEKTKLKVGFIPITCATPIIMAHPMGFYAKH
                     GLDVEVVKTAGWAVIRDKTLNKEYDAAHMLSPMPLAITLGAGATAVPYTMPAVENING
                     QAITLAVKHKDRRDPKTWKGFKFAVPFDYSMHNYLLRYYVAEHGLDPDTDIQIRAVPP
                     PEMVANLRADNIDGFLAPDPVNQR"
     gene            complement(905..1687)
                     /locus_tag="VGO02_02745"
     CDS             complement(905..1687)
                     /locus_tag="VGO02_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEV7476634.1"
                     /translation="MSDEPQVTNSELQAYADGRLNDERSRAVEAWLAARPDDAERIAA
                     YRRLAEELRRAYDPVLDEPLPDWLGGTRAPRPRWHALARVAGWMALGAALGAIAGWEV
                     RDARPVAVSRGEPGAIMAYRAAVAHATYSPEVRHPVEVGADQEAHLVAWLSKRLGTQL
                     RAPKLESVGYSLVGGRLLPGDTGPVAHFMYQSGQGSRITLYVRSDMTGNRETAFRYAR
                     EGSVGVFYWVDSKIGYAISSADLGKEQLFAIANAAYQQLNPP"
     gene            complement(1684..2181)
                     /locus_tag="VGO02_02750"
     CDS             complement(1684..2181)
                     /locus_tag="VGO02_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV7476635.1"
                     /translation="MSEAERLVELIPRLRRYARALVGDRAAADDLVQDTLERAWAKLH
                     LYRRGTDLRAWLFTVMHNVHVNKVRATRATDTLDDEMPELAQRAAQGDALMVRDLDRS
                     ISRLPAEQRSVLLLVTLEDMSYDEVARTLGIPIGTVMSRLSRAREKLRAMMLGQPSAK
                     LKVVK"
     gene            complement(2225..3064)
                     /locus_tag="VGO02_02755"
     CDS             complement(2225..3064)
                     /locus_tag="VGO02_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEV7476636.1"
                     /translation="MLFFCAVAAAQGDAQRGLYLSKAGGCIGCHTQATKDPKEAVPYA
                     GGRALKTPFGTFYGPNITPHPTAGIGKWSEADFIRAMREGRRPDGAHYFPAFPYPSFT
                     GITDADLRDLWAYLRGLPPSARASQEHELGILYRWRFLVGFWKWLYFTPGPLLQDPKR
                     PPAVNRGHYLVDALGHCGECHTPRNFMGGPKRDRYLAGTDKGPEGKAVANITPARLKS
                     WSDAELRDFLQTGMTADGDVTAETMGEVVTNTTSQLTPGDLAALIAYLRSVPPVESEK
                     KKN"
     gene            complement(3117..3569)
                     /locus_tag="VGO02_02760"
     CDS             complement(3117..3569)
                     /locus_tag="VGO02_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEV7476637.1"
                     /translation="MRKTFIAAGAAFALAIPLTVLAQAKPETLVKQRQAAMTLQGKYI
                     GPIGAMVNGKIPYNADVVARNATYLENLSQMPWDGFHESTKGEKSAARPEIWTEPAKF
                     QEAADRLQAETVKLATAARAKDEAGVKAQFPAVGKACGACHESFRVKQ"
     gene            complement(3634..4251)
                     /locus_tag="VGO02_02765"
     CDS             complement(3634..4251)
                     /locus_tag="VGO02_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEV7476638.1"
                     /translation="MIRVCALAVGLFFAAASAFAQDAGKAQSIAAQVCAACHAADGNS
                     IGPANPKIAGQHPEYLHKQLADFKGQSGKKPVRDNAVMTSMVANLSDADMKNLAAYFS
                     GQKLKPAAAADKELAALGQKVWRGGNAQTGVPACAGCHGPTGAGIPAQYPRVAGQYSE
                     YVATQLRAFKEGGRANDPNGMMRGVAARMSEREIKAVAEYAAGLR"
     gene            4333..4884
                     /gene="yihA"
                     /locus_tag="VGO02_02770"
     CDS             4333..4884
                     /gene="yihA"
                     /locus_tag="VGO02_02770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEV7476639.1"
                     /translation="MLSAGRASDLPPRGPPEVAFAGRSNVGKSSAINALLGRKGLART
                     SKTPGRTQTINFYELADGMRLVDLPGYGYARVPKLMREEWKALVGAYLESRTPAAVVV
                     IMDARHPATDLDRQLLAWLGERRVLVLLSKADKLSRAEQKKTLQHASSLAADVRLFSS
                     VTRQGVEECRDLLAGWLQADPLE"
     gene            5054..6130
                     /gene="hemB"
                     /locus_tag="VGO02_02775"
     CDS             5054..6130
                     /gene="hemB"
                     /locus_tag="VGO02_02775"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEV7476640.1"
                     /translation="MKVYGQFPSVRMRRMRRNEFSRRLMRETVLTADDLIYPVFVLDG
                     KSRTENVASMPGVERMTIDRLLPVAEQCVKLRIPVLALFPVIEQRLKSADGREAFNPR
                     GLVPRATAELKKRFPELGIMSDVALDPYTSHGQDGVIDRSGYILNDTTLEVLVRQALT
                     QAEAGVDIVAPSDMMDGRIGRIRAALEKAGHIHTRIMAYSAKYASGFYGPFRDAVGSA
                     KNLGKSDKKTYQMDPANSDEALWEVGLDLAEGADMVMVKPGLPYLDVLRRVKDEFRAP
                     TYVYQVSGEYAMLRAAIGNGWLPESCVMETLLAFKRAGADGILTYFALDAARELQLGV
                     NSPRRGKASSRTARRSGSRSRAAP"
     gene            complement(6054..>6807)
                     /locus_tag="VGO02_02780"
     CDS             complement(6054..>6807)
                     /locus_tag="VGO02_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HEV7476641.1"
                     /translation="ALSMFGLYELKLPGFLHQRLDEAQRRLPGGRIASVASMGALSAV
                     IVSPCVAAPLAGALLYIAQTRDVALGGAALFTMALGMGIPLIAVGVSEGALLPKSGPW
                     LNRVKQFFGVLLLAIAAWIVSPLFLSGKGETKFVRVDSLAELDARLAAPGKPVMLDFY
                     ADWCVSCKEMEAFTFSQPPVKKQLDGMLLLQVDVTANTAEHKALLKRFSLFGPPGIIF
                     FDAQGREIKGLRVIGYQNAERFAKTLSLVEGN"
BASE COUNT         1131 a         2234 c         2289 g         1153 t
ORIGIN      
        1 gcgctggttc accggatccg gcgcgaggaa gccgtcgatg ttgtcggcgc gcaggttggc
       61 caccatctcc ggcggcggca cggcgcggat ctggatgtcg gtgtcgggat ccaggccgtg
      121 ctcggcaacg tagtagcgca gaaggtagtt gtgcatcgaa tagtcgaagg gcaccgcgaa
      181 cttgaatccc ttccaggtct tcgggtcgcg cctgtccttg tgcttcacgg cgagcgtaat
      241 cgcctggccg ttgatgttct cgaccgccgg catggtgtag ggaaccgcgg tcgcgcccgc
      301 gccgagcgtg atggcgagcg gcatcggcga aagcatgtgc gcggcgtcgt attccttgtt
      361 gagcgtcttg tcgcggatca ccgcccagcc ggcggtcttc accacctcga cgtcgaggcc
      421 gtgcttggcg tagaagccca tcgggtgcgc catgatgatc ggcgtggcgc aggtgatcgg
      481 gatgaagccc actttgagct tggtcttttc gggcttgccc gcgccttgcg caaaagcctc
      541 ctttgccgcg ccgagcggaa acacggtgga caacgcggcg agcgcggtgc cggcaccgac
      601 cgcctgcagg aaccggcggc gcagcgctga atctggaagc agggcgcgca gcagcgcctc
      661 ctcgacgatg cgctcctcgg cgagcgcggc ggcgtgctcg gtgtcggtac ggtgcttacc
      721 gcacatgcag ctgaaatgac ggtgcatcgc acggcctcct ggctagtgga tgccgacgtt
      781 ttgcaccaac cgtgccaacg cgctaagcgc ttgtcttatc tgggttcaac gcggacacgc
      841 gccgcccgca tcacaaacac ggtgcacgct gccaggtcgg cgcaccgccc gggtgcgccg
      901 ggggctaggg gggattgagc tgctggtacg cggcgttcgc gatggcgaac agctgctcct
      961 tgccgaggtc ggcggaggag atggcgtagc ctatcttgct gtcgacccaa tagaacacgc
     1021 cgacactgcc ttcgcgcgcg tagcggaaag cggtctcgcg gttgccggtc atgtcggagc
     1081 gaacatacag cgtgatgcgc gacccctggc cgctctggta catgaaatgc gccaccggcc
     1141 cggtgtcgcc gggcagcaag cggccgccga ccaggctgta gcccactgat tcgagcttgg
     1201 gcgcgcgcag ctgcgtgccg agccgcttgg aaagccaggc caccagatgc gcttcctggt
     1261 ctgcgccgac ctcgaccgga tggcgcacct cgggcgaata ggtggcgtgc gccaccgccg
     1321 ctcggtaggc catgatcgcg ccgggctcgc cgcgcgaaac cgctaccgga cgcgcgtcgc
     1381 gcacctccca gccagcgatt gcgccgagcg ctgcgccgag cgccatccag cccgcgaccc
     1441 gcgccagggc atgccagcgc ggacgcggcg cgcgcgtgcc gccgagccag tccggcagag
     1501 gctcgtcgag caccggatcg taggcgcggc gcagctcctc agctagccgg cggtaggccg
     1561 cgatgcgctc cgcgtcatcg ggtcgggcgg caagccaggc ctcgacggcg cggctgcgct
     1621 cgtcgttcag cctgccgtcg gcgtaggcct gcagctcgga attggtgacc tgcggctcgt
     1681 cgctcatttc accaccttca gcttggccga cggttgcccg agcatcatgg cgcgcagttt
     1741 ctcgcgcgcg cgcgacaggc gcgacatgac cgtaccgatc gggataccga gggtacgcgc
     1801 cacctcgtcg tagctcatgt cctcgagcgt caccagcagc agcaccgagc gctgctcggc
     1861 tggcaggcgc gagatcgagc ggtcgaggtc gcgcaccatc agcgcgtcgc cctgcgccgc
     1921 gcgctgcgcg agctcgggca tctcgtcgtc gagcgtgtcg gtggcgcgcg ttgcgcgcac
     1981 tttgttgacg tgcacgttat gcatcacagt gaaaagccat gctcgcaggt cggtgccgcg
     2041 gcgataaaga tgcagcttcg cccaggcgcg ctcgagggtg tcctgcacca gatcgtcggc
     2101 ggcggcgcgg tcccccacca gcgcgcgggc atagcgccgc agccgcggaa tcagctcgac
     2161 caggcgctcg gcttcgctca tcgggataac aagtctaccg cgaccgctat tccgtaaaac
     2221 aacgtcagtt cttcttcttc tcgctctcga cggggggaac cgagcgcagg taggcgatca
     2281 gcgccgccag gtcgcccggg gtgagctggc tggtggtatt ggtcaccacc tcgcccatgg
     2341 tctccgcggt gacgtccccg tcggctgtca tgcccgtctg cagaaaatcg cgcagctcgg
     2401 catcgctcca gctcttcagc ctggcggggg tgatgttggc cacggccttg ccctcgggcc
     2461 ccttgtcggt tcccgcgagg taccggtcgc gcttgggccc gcccatgaaa ttgcgcggcg
     2521 tgtggcactc gccgcaatgc ccgagcgcgt cgaccagata gtgaccgcga ttcactgcgg
     2581 ggggccgctt cggatcctgc agcagcggcc cgggagtgaa gtagagccac ttccagaagc
     2641 cgacgaggaa acgccagcgg tagaggatgc cgagttcgtg ctcctgactg gcgcgcgcgc
     2701 tcggcggcag gccgcgcagg taggcccaga gatcgcgcag atcggcatcg gtgatcccgg
     2761 tgaaggacgg gtagggaaaa gccggaaagt aatgcgcgcc gtcgggacgc cggccctcgc
     2821 gcatcgcgcg aatgaaatcc gcttcgctcc acttgccgat gccggcggtg ggatgcggcg
     2881 tgatgttcgg gccgtagaaa gtgccgaagg gcgtcttcag cgcacgtcca ccggcatagg
     2941 ggacggcctc cttcggatcc ttcgtggcct gggtatggca gccgatgcag ccgcccgcct
     3001 tcgacagata caggccgcgc tgcgcatcgc cctgcgccgc cgcgaccgcg caaaaaaaga
     3061 gcaaccccag aagcaagagc cccgcagcag cggggctctt tacaggcagc aagcggttat
     3121 tgcttgacgc ggaaactctc gtggcaggct ccgcaggcct tgcccaccgc ggggaactgc
     3181 gccttgacgc ccgcctcgtc cttggcgcgc gccgcggtag cgagcttcac ggtctcagct
     3241 tgcaggcggt cggcggcttc ctggaacttc gccggctcgg tccagatctc gggacgcgcg
     3301 gccgatttct cgcccttggt gctctcatgg aacccgtccc acggcatttg cgacaggttc
     3361 tcgaggtagg tggcgttgcg cgccacgacg tcggcgttat acggaatctt gccgttgacc
     3421 atcgcgccga tcgggccgat gtacttgccc tgcagggtca tggctgcctg gcgttgcttg
     3481 accagggtct cgggcttcgc ctgggccagc acggtgagcg ggatggccag ggcgaaagca
     3541 gcaccggcgg cgatgaaagt ttttctcatt accaatctcc tcgggttggg tcgttcagct
     3601 aacaacgcct gcctgcactt tattcaacgt gatctatcga agtccggcgg catattccgc
     3661 cacagccttg atctcgcgct cgctcatgcg cgcggcaacg ccgcgcatca tgccgtttgg
     3721 atcgttggcg cggccgcctt ccttgaaggc gcgcaactgg gtggcaacat actctgagta
     3781 ctggcccgct acccggggat actgcgccgg gatcccggcc cccgtcgggc cgtggcagcc
     3841 cgcgcaggcc ggcacaccgg tctgggcgtt gccgccccgc cagaccttct ggccaagcgc
     3901 cgccagttcc ttgtcggccg ccgcggcggg cttgagcttc tgtcccgaga agtaggctgc
     3961 caggtttttc atgtcggcgt cggacaggtt ggcgaccatg ctcgtcatga ccgcgttgtc
     4021 gcgcaccggc ttcttcccgc tctggccttt gaagtccgcg agctgcttgt gcaggtattc
     4081 cgggtgctga ccggcgatct tcgggttcgc cggcccgatg ctgttgccgt cggcggcgtg
     4141 acacgccgcg cacacctgcg cggcgatgct ttgcgctttg cccgcatcct gggcgaaagc
     4201 cgacgcggcc gcaaagaaca agccgacggc aagcgcgcat actcggatca tcccttcgcc
     4261 ccctgcgttc gaaaggcgcg attttattag aatttccgcc tcccgatgaa gaccttttcc
     4321 caagcgcggt ttatgctctc cgcaggccgg gccagcgacc tgcccccgag gggccctccg
     4381 gaggtggcat ttgctggccg ttccaacgtc ggcaagtcga gcgccatcaa cgcactcctc
     4441 ggacgcaagg gcctggcgcg cacgagcaag acgccgggcc gcacgcaaac gatcaacttt
     4501 tacgagctgg cggacggcat gcgtctggtc gacctgcccg gttatggata tgcgcgcgtg
     4561 cccaagctga tgcgcgagga gtggaaagcg ctggttggcg cgtacctcga atcgcgcacc
     4621 ccggcggcgg tcgtggtgat catggatgcg cggcacccgg ccaccgatct cgaccggcag
     4681 ctcctcgcgt ggctcgggga acggcgggtg ctggtgctgc tgtcgaaggc ggacaagctg
     4741 tcgcgcgccg agcagaaaaa aaccctgcag cacgcaagct ctttggcggc ggacgtgcgc
     4801 ctgttttcga gcgttacgcg acagggtgta gaggaatgcc gggacctgct tgcagggtgg
     4861 ctgcaagcag atccgctgga ataaaaggcc cccggtaaag gggatataaa ccgggggcga
     4921 aagcgcctta acttgggatt aaggcaaccc gctcagggag gagaagcggg agacgatcaa
     4981 ctcgtctgaa ggtaagattc gcctccccgg ggaaaagttc caacccgtta aaaagctcaa
     5041 tatcgtcagc cccatgaagg tctacggtca gtttccatcg gtgcgcatgc gtcgcatgcg
     5101 gcgcaacgaa ttctcgcggc ggctgatgcg cgagaccgtg ctgacggcgg acgacctgat
     5161 ctacccggtg ttcgttctcg acgggaaatc ccgcaccgag aatgtggctt ccatgccggg
     5221 ggtcgagcgc atgacgatcg acaggctgct gccggtcgcc gagcagtgcg tgaagctgcg
     5281 cattcccgtg ctcgcgctgt tcccggtgat cgagcagcgg ctcaagagcg ccgatggacg
     5341 cgaggcgttc aatcccaggg gcctcgtgcc gcgcgccacc gccgagctga aaaagcgctt
     5401 ccccgagctc ggcatcatga gcgatgtcgc gctggatccg tacacctcgc acggccagga
     5461 cggcgtaatc gatcgcagcg gctatatcct caacgacacc actctcgagg ttctcgtccg
     5521 gcaggcgctg acgcaggccg aggccggcgt cgatatcgtc gcgccttcgg acatgatgga
     5581 cgggcgtatc ggccgcatcc gcgccgcgct cgagaaggcc ggccacatcc acaccaggat
     5641 catggcgtac tcggcgaaat acgcctcggg cttctacggc ccgttccgcg acgcggtcgg
     5701 ctcggcgaag aacctcggca agagcgacaa gaagacctac cagatggacc cggcgaattc
     5761 cgacgaggcg ctgtgggaag tcggcctcga cctcgccgag ggcgcggaca tggtgatggt
     5821 caagcccggc ctgccctacc tcgacgtcct gcggcgcgtg aaggatgaat tcagggcgcc
     5881 gacctacgtc taccaggtgt cgggcgagta cgcgatgctg cgcgcggcga tcggcaatgg
     5941 ctggctgccg gagtcctgcg tgatggaaac gctgctggcg ttcaagcgcg ccggcgccga
     6001 cggaatcctg acctacttcg cgctggacgc ggctagggaa ttgcagttag gggtcaattc
     6061 ccctcgacga gggaaagcgt cttcgcgaac cgctcggcgt tctggtagcc gatcacgcgc
     6121 agccccttga tctcgcggcc ctgcgcgtcg aagaagatga tgccgggcgg accgaagagc
     6181 gagaagcgct tgagtagcgc cttgtgctcg gccgtgttcg ccgtcacgtc aacctgcagg
     6241 agcagcatac cgtcgagctg tttcttcacc ggcggctggc tgaaggtgaa agcctccatc
     6301 tccttgcacg acacgcacca gtcggcatag aagtcgagca tcaccggctt gcccggcgcg
     6361 gcgagcctgg cgtcgagctc ggccaggcta tcgacgcgca cgaacttcgt ctcccccttg
     6421 ccgctcagga acaggggcga aacgatccat gccgcgatcg ccagcagcag cacgccgaag
     6481 aactgcttga cgcgattgag ccagggtcct gatttgggca gcaaggcacc ctcggagaca
     6541 ccgaccgcga tcagcggaat gcccatgccg agcgccatgg tgaagagcgc cgcgccgccg
     6601 agcgcgacgt cgcgcgtctg ggcgatgtag agcagagcgc cggcgagcgg cgccgcgacg
     6661 cacgggctga cgatcaccgc cgaaagcgcg cccatgctcg cgaccgaagc aatccgcccc
     6721 cccggcagcc gccgctgcgc ctcatcgagg cgctgatgca ggaagccggg cagcttgagc
     6781 tcgtacaggc cgaacatgga gagcgcg
//
LOCUS       DASYJA010000130         1517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_111700, whole genome shotgun sequence.
ACCESSION   DASYJA010000130 DASYJA010000000
VERSION     DASYJA010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111700"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VGO02_02785"
     CDS             <1..312
                     /locus_tag="VGO02_02785"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEV7476642.1"
                     /translation="LSITNHWWSGFSLNANAGKWNALPADIRAVITRHADAAALAQRQ
                     DVAKIDAGALEVLRAKGMTVNETDTSGFRRQLGAFYARWKAVYGDKAWALLEARVGKL
                     I"
     gene            331..1296
                     /locus_tag="VGO02_02790"
     CDS             331..1296
                     /locus_tag="VGO02_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7476643.1"
                     /translation="MATLILLAVFSAASYSQITLRVKMFPGAQALPVIAAASQGIFER
                     HGLKVEVLFTVNSQEQRDGLASGDFQVAQAAVDNAVAMVELAKQDVIIVTGGDSSMNE
                     FFVQPDVRAYTDLRGQTLLVDAPNTAYALQLKKILLMNGLKEGADYKIHPIGGTALRL
                     KGLRENKEYKAAMLNPPFSIDAKGAGLKSMGRAADLLGAYQATGTFVLRSWAKANGDT
                     LERYIAGFIEGVRWAVAPANKAAAVKLLSERLKVTPEVAAQTWDLLSDPKFGLATDGR
                     FDMQGFRNVLALRAEIEGSWGGKAPGPERYLDLSYYDRALKRVSP"
     gene            complement(1286..>1517)
                     /locus_tag="VGO02_02795"
     CDS             complement(1286..>1517)
                     /locus_tag="VGO02_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7476644.1"
                     /translation="KYDRPEAMKLEPFGEDSVQRLIVGPPGTVLEKLQEVIDATGMNY
                     LLCIFSFGNLPPQAALRSLGMFAGEVMPKLRD"
BASE COUNT          271 a          511 c          515 g          220 t
ORIGIN      
        1 ctcagcatca ccaaccactg gtggtccggc ttctcgctca acgccaacgc cgggaagtgg
       61 aacgcgctgc ccgcggacat ccgggccgtc atcacccgcc acgccgacgc cgccgcgctc
      121 gcgcagcggc aggacgtggc gaagatcgac gccggcgcgc tcgaggtgct gcgcgccaag
      181 ggaatgacgg tgaacgagac cgacaccagc ggcttccggc gccagctcgg cgccttctat
      241 gcgcgctgga aggcagtcta cggcgacaag gcgtgggccc tgctcgaggc acgggtaggc
      301 aaactgattt aaggagaaac catgattcga ttggcaaccc tcatccttct ggcggtgttt
      361 tcagccgcta gctattcgca aatcaccctg cgggtaaaga tgttccccgg cgcgcaggcg
      421 ctgccggtca tcgccgccgc ctcgcagggc atcttcgagc gccatggcct gaaggtcgag
      481 gtgctgttca ccgtgaactc gcaggagcag cgcgacggcc tcgcctcggg cgacttccag
      541 gtcgcgcagg ccgcggtcga caacgccgtc gcgatggtcg agctggcgaa gcaggacgtg
      601 atcatcgtga ccggcggcga cagcagcatg aacgagttct tcgtgcagcc cgacgtgcgc
      661 gcctacaccg acctgcgcgg ccagacgctg ctggtcgacg cgccgaacac cgcctatgcg
      721 ctgcagctga agaagatcct gctgatgaac gggttgaagg aaggcgccga ctacaagatc
      781 catccgatcg gcggcaccgc gctgcgcctc aaggggctgc gcgagaacaa ggagtacaag
      841 gcggcgatgc tcaacccgcc gttctcgatc gacgccaagg gcgcggggct gaagagcatg
      901 ggccgcgcgg cggacctcct tggcgcctac caggcgaccg gcacgttcgt cctgcgcagc
      961 tgggcgaagg ccaacggcga cacgctcgag cgctacatcg ccggcttcat cgagggcgtg
     1021 cgctgggcgg tcgcgccggc caacaaggcg gccgcagtca agctgctctc cgagcggctc
     1081 aaggtcacgc ccgaagtggc ggcgcagacc tgggatctgc tgagcgatcc gaagttcggc
     1141 ctggcgaccg acggccgctt cgacatgcag ggcttccgca acgtgctcgc gctgcgcgcc
     1201 gagatcgagg gcagctgggg cggcaaggcg cccgggcccg agcgctacct ggacctcagc
     1261 tactacgaca gggcgctgaa gcgcgtcagt ccctgagctt gggcatcacc tcgccagcga
     1321 acatcccgag cgagcgcagc gcggcctgcg gcggcaagtt gccgaaggag aagatgcaca
     1381 gcaggtagtt catgccggtc gcgtcgatca cctcctgcag cttctccagc acggtgcccg
     1441 gcgggccgac gatcaggcgc tgcaccgagt cctcgccgaa gggctcgagc ttcatcgcct
     1501 cggggcggtc gtacttg
//
LOCUS       DASYJA010000131         2406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2563766, whole genome shotgun sequence.
ACCESSION   DASYJA010000131 DASYJA010000000
VERSION     DASYJA010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2563766"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1590)
                     /locus_tag="VGO02_02800"
     CDS             complement(19..1590)
                     /locus_tag="VGO02_02800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV7476645.1"
                     /translation="MNKPRRSPDLSATAERGALTEALEALRASEARFRAVIDSANEGI
                     LVYDSALMVTAGNAAAERIIGLPLAEMVGKPGFTSLLPCVREDGTPLAPEDRPTRITV
                     RSGKPLTGQVIGIVRPDRSITWLDVNTGFLQRPGEREFYGIVSTISDVTARRKAELAL
                     RDSEERFRQTFQLAASGICEVVDGRFVRVNRSLCEILGYAEDHLLGMTVKEVSHPEDR
                     DVTDAERARIHAGEIDSARFEKRYVRADGRVVWCELAVALVRSVDGHPLYEIAVFDDV
                     TERKDGEAALRQAHEELKRSNAELEQFAYVASHDLQEPLRMVSSYTQLLMRRYGDRLD
                     GDAREFTAYIVDGATRMKQLIEDLLAYSRVGTKGKEFKPVPVEAALRRAIVNLRAAIE
                     ESGAAVTYDTLPVVQGDDVQIAQLFQNLIGNALKFRSASVPRIHVSCIQGQAEHEIVV
                     ADNGIGIEAQYFERIFMVFQRLHNKSEYPETGIGLAICKKVVERHNGRIWVESQPGGG
                     SSFHFTLPKMGEGEP"
     gene            complement(1690..>2406)
                     /locus_tag="VGO02_02805"
     CDS             complement(1690..>2406)
                     /locus_tag="VGO02_02805"
                     /inference="COORDINATES: protein motif:HMM:NF020135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEV7476646.1"
                     /translation="TAVAFVQNGIPWWYGHGVEGKVPARLPGFSRLDPGGELARAIAP
                     GRVIGAVVFSANEVDAPGVIRNLVPGRNMLVVGEPDDRESPRIQTLRKALDAADMHSP
                     PTRDIRQVIWSKLVLNLGTSMLCTLTGETVGGVRGDAELPAIANRLKAEGQAIAAAYG
                     IAAEGAPERPGGGQSSGQIAHKPSMMQDYERGRPMEIDAQIMMPLDFARAAGVAAPTL
                     EALAPLLAHKARAKGLYPRN"
BASE COUNT          339 a          831 c          826 g          410 t
ORIGIN      
        1 gcccgtttct ctcgaccatc atggctctcc ttctcccatc ttaggtagcg tgaaatggaa
       61 gctgctgccc ccgccgggct gcgactccac ccagatgcgg ccgttgtgcc gctcgacgac
      121 cttcttgcag atggccagcc cgatccccgt ctctgggtac tcgctcttgt tgtgcaggcg
      181 ctggaacacc atgaagatgc gctcgaagta ctgcgcctcg atgccgatgc cgttgtccgc
      241 tacgacgatt tcgtgctcgg cttgtccttg aatacaggaa acatggattc tgggaaccga
      301 cgccgagcgg aacttgagcg cgttgccgat caggttctgg aacagctgcg cgatctgcac
      361 gtcgtcgccc tggaccaccg gcagcgtgtc gtaggtgacc gccgcgcccg actcctcgat
      421 cgccgcgcgc aggttgacga tggcgcggcg cagcgccgcc tcgaccggca ccggcttgaa
      481 ctccttgccc ttggtgccga cgcgcgaata ggcgagcaga tcctcgatca gctgcttcat
      541 gcgcgtcgcg ccgtcgacga tgtaggcggt gaactcgcgc gcgtcgccgt cgaggcgatc
      601 gccgtagcgg cgcatcagca gctgggtgta gctcgacacc atgcgcagcg gctcctgcag
      661 gtcgtgcgag gcgacgtagg cgaactgctc gagctcggcg ttggagcgct tgagctcctc
      721 gtgcgcctgg cgcagcgccg cctcgccgtc cttgcgctcg gtcacgtcgt cgaacaccgc
      781 gatctcgtac agcgggtggc cgtcgacgct gcgcaccagc gctaccgcga gctcgcacca
      841 caccacgcgg ccgtcggcgc gcacgtagcg cttctcgaag cgcgccgagt cgatctcgcc
      901 ggcgtggatg cgcgcgcgct cggcgtcggt gacgtcgcgg tcctcgggat gcgagacttc
      961 cttcaccgtc atgccgagca ggtggtcctc ggcatagccg agaatctcgc acaggctccg
     1021 gttgacgcgc acgaagcgcc cgtcgaccac ctcgcagatg cccgaggcgg cgagctggaa
     1081 ggtctggcgg aagcgctcct cgctgtcgcg cagcgccagc tcggccttgc ggcgcgcggt
     1141 gacgtcgctg atggtggaga cgatgccgta gaactcgcgc tcgccggggc gctgcaggaa
     1201 gccggtgttg acgtccagcc acgtaatcga ccggtcgggg cgcacgatgc cgatcacctg
     1261 cccggtgagc ggcttgccgc tgcgcaccgt gatgcgcgtc ggccggtcct cgggagcgag
     1321 cggcgtgccg tcctcgcgca cgcacggcag cagcgaggta aagcccggct tgccgaccat
     1381 ctcggcgagc ggcaggccga tgatgcgctc ggccgcggcg ttgccggcgg tgaccatgag
     1441 ggcgctgtcg tacaccagga tgccctcgtt cgccgagtcg atcaccgcgc ggaagcgcgc
     1501 ctcgctcgcg cgcagcgcct cgagggcctc ggtgagcgcg ccgcgctcgg cggtcgcgct
     1561 caggtcgggg ctgcggcgcg gcttgttcat ctggctcaaa ttctaatctc ccccactgcg
     1621 cgccgagcac tttccgggcg cccgtgagca cttttacaag cgggacgccg ggataaggcc
     1681 cggcagggct tagttccttg ggtacaggcc cttggcgcgg gccttgtggg cgagcagcgg
     1741 cgccagcgct tcgagcgtcg gcgcggcgac gcctgcggcg cgcgcgaagt cgagcggcat
     1801 catgatctgc gcgtcgatct ccatcggccg gccgcgctcg tagtcctgca tcatggaagg
     1861 cttgtgcgcg atctggcccg agctctggcc gccgccgggg cgctcgggcg cgccctcggc
     1921 agcgatgccg taggcggcgg caatcgcctg gccttcggcc ttcagccggt tggcgatcgc
     1981 cggcagttcg gcatcgccgc gcacgccgcc caccgtttcc ccggtaagcg tgcacagcat
     2041 cgacgtgccc aggttgagca ccagcttcga ccagatcacc tggcggatat ccctcgtcgg
     2101 cggcgaatgc atgtccgcgg catcgagggc ttttcgcagg gtctgtatgc ggggtgattc
     2161 ccggtcatcc ggttccccga cgaccagcat gttgcgcccg ggcacgaggt tgcggatgac
     2221 gccgggcgcg tcgacctcgt tggctgaaaa cacgaccgcg ccgatcacgc gtccgggggc
     2281 gatggcgcgc gccagctcgc cgccgggatc gagacgcgag aagccgggca gccgagccgg
     2341 tactttccct tcgacgccgt ggccgtacca ccagggaatg ccgttctgca cgaacgcgac
     2401 ggcagt
//
LOCUS       DASYJA010000132         2237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3018480, whole genome shotgun sequence.
ACCESSION   DASYJA010000132 DASYJA010000000
VERSION     DASYJA010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3018480"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VGO02_02810"
     CDS             <1..198
                     /locus_tag="VGO02_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476647.1"
                     /translation="QALAIDHALADPRKIHADLVELARSSAAAGDRDAARGYLERAQT
                     VSHAARDPRGLEEMEVQLRLR"
     gene            complement(231..1166)
                     /locus_tag="VGO02_02815"
     CDS             complement(231..1166)
                     /locus_tag="VGO02_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476648.1"
                     /translation="MSEELNRTLVCSVLFIDIVDYSKHDVAEQVRLKHRFNSVLSSAL
                     GHVELDERVVVDTGDGAAIAFRGDPERALYVALEVFDANVEDLPVRMGINLGPVSLMR
                     DINGADNVIGDGINVAQRVMGFAARGELLASRSFYEVVSRLSDDYANMFKDEGVRLDK
                     HKRSHDVYSVSQAVRVARRVAEAQSRAKTQRPGAGYTVIGHTPAQVFDAGTHYMVSGY
                     SESSVREAVNKLAGQGGKLITDVSKVGAKWLASVENPHAAGASVREFGFKHVVSAPTR
                     EAVEVKVRELLEMGATLVQEAELADGVWTAVCEKT"
     gene            complement(1183..>2237)
                     /locus_tag="VGO02_02820"
     CDS             complement(1183..>2237)
                     /locus_tag="VGO02_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003271648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7476649.1"
                     /translation="PAVILLAGLQGSGKTTTSGKLARFLKESKKKVALVSCDVYRPAA
                     IEQLKAVAAQVGADFISSSPSEKPVEIAKRALEYARTHYMDVLIVDTAGRLAIDEQMM
                     QEIAALHAELKPAETLFVVDAMQGQDAVNVARAFSQRLPLTGVILTKLDGDSRGGAAL
                     SVRHVTGKPIKFAGTGEKLDGLEPFHPERMAARVLGMGDIVSLVEEAHARIDLEEAKR
                     VAEKVKKGKGFDLEDFKQQIAQMKKMGGVASLLDKLPAQLVGNINPAQLQDDKQLRRI
                     EGIINAMTPQERAKPELIKASRKRRIAAGAGVPVQAVNQLLNQFEQMQKMMKMMKGGN
                     MARMMRGMKGMLPGSR"
BASE COUNT          325 a          797 c          721 g          394 t
ORIGIN      
        1 caggccctcg ccatcgacca tgcgctcgcc gacccgcgca agatccacgc cgacctggtc
       61 gagctggcgc gctcgagcgc ggccgccggc gaccgcgatg cggcgcgcgg ctacctcgag
      121 cgcgcgcaga cggtgagcca cgccgcgcgc gacccgcgcg gcctggagga aatggaagtg
      181 cagctgcgcc tgcggtaagg agcgctagaa gccgcgctca aggcgccgcg ctaagtcttc
      241 tcgcagaccg cggtccacac gccgtcggcc agctccgcct cctgcaccag ggtggcgccc
      301 atctcgagca gctcgcgcac cttgacctcc accgcttcgc gcgtcggcgc cgagacgacg
      361 tgcttgaagc cgaactcccg cacgctcgcg cccgccgcgt gcggattttc gacgcttgcc
      421 agccacttcg cgccgacctt cgagacgtcg gtgatgagct tgccgccctg ccccgcgagc
      481 ttgttcaccg cctcgcgcac gctcgactcg ctgtagcccg agaccatgta gtgcgtgccg
      541 gcatcgaaca cctgcgccgg ggtatggccg atgaccgtgt acccggcccc ggggcgctgg
      601 gtcttcgccc gggactgcgc ctcggcgacg cgccgcgcga cgcgcacggc ctggctcacc
      661 gagtagacgt cgtgcgagcg cttgtgcttg tcgaggcgca cgccctcgtc cttgaacatg
      721 ttggcgtagt cgtcggagag ccgcgacacc acctcgtaga acgagcgcga cgccagcagc
      781 tcgccgcgcg cggcgaagcc catgacgcgc tgcgcgacat tgatgccgtc gccgatcacg
      841 ttgtccgcgc cgttgatgtc gcgcatcagc gagaccgggc cgaggttgat gcccatgcgc
      901 accggcaggt cctcgacgtt ggcgtcgaat acctcgagcg ccacgtagag ggcgcgctcc
      961 gggtcgccgc ggaaggcgat ggcggcgccg tcgccggtgt cgaccaccac gcgctcgtcc
     1021 agctcgacgt gcccgagcgc gctcgacagc acgctgttga agcggtgctt gaggcgcacc
     1081 tgctcagcca cgtcgtgctt ggagtagtcg acgatgtcga tgaagagcac gctgcagacg
     1141 agcgtgcggt tgagttcttc gctcacgggg tgcggggcgc cttcagcgcg aaccgggcag
     1201 catgcccttc atgccgcgca tcatgcgcgc catattcccg cctttcatca ttttcatcat
     1261 cttttgcatc tgctcgaact ggttgagcag ctggttcacc gcctgcaccg gcacgccggc
     1321 gccggcggcg atgcgccgct tgcgcgaggc cttgatcagc tcgggcttgg cgcgctcctg
     1381 cggcgtcatc gcgttgatga tgccttcgat gcgccgcagc tgcttgtcgt cctgcagctg
     1441 ggcggggtta atatttccga caagttgcgc aggcaacttg tcgagcaacg aggcgacgcc
     1501 gcccatcttc ttcatctgcg cgatctgctg cttgaagtcc tcgaggtcga agcccttgcc
     1561 cttcttcacc ttctcggcga cgcgcttcgc ctcctcgagg tcgatgcgag cgtgcgcttc
     1621 ctcgaccagc gacacgatgt cgcccatgcc gagcacgcgc gccgccatgc gctccggatg
     1681 gaacggctcc aggccatcga gcttctcgcc ggtgccggcg aacttgatcg gcttgccggt
     1741 gacatggcgc acggagagcg ccgcgccgcc gcgcgagtcg ccgtcgagct tggtgaggat
     1801 cacgccggtc agcggcagcc gctggctgaa ggcgcgcgcc acgttgaccg cgtcctggcc
     1861 ctgcatggcg tcgaccacga acagcgtttc ggcgggcttc agctccgcgt gcagcgccgc
     1921 gatctcctgc atcatctgct cgtcgatcgc caggcgcccc gcggtatcga cgatcagcac
     1981 gtccatgtag tgcgtgcgcg cgtactcgag cgcccttttt gcgatttcaa cgggtttttc
     2041 agaaggcgac gaagaaataa agtccgctcc aacctgagca gccaccgcct tcagctgctc
     2101 gatcgccgct ggccggtaga cgtcgcagga caccagcgcc acctttttct tgctctcttt
     2161 caggaagcgt gccagcttgc cgctggtggt ggtcttgccc gagccctgca ggccggccag
     2221 caggatcacc gccggcg
//
LOCUS       DASYJA010000133         2652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2200900, whole genome shotgun sequence.
ACCESSION   DASYJA010000133 DASYJA010000000
VERSION     DASYJA010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2200900"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /gene="dctP"
                     /locus_tag="VGO02_02825"
     CDS             complement(<1..979)
                     /gene="dctP"
                     /locus_tag="VGO02_02825"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEV7476650.1"
                     /translation="MLKTIAVLAALAGALPAAAQDTISATHVFPASLIYSRSFLEFVK
                     KANEAGKGVFTIQVRGGPEAIGMMEQPGAVRSGVVDMVYSACAFYAAVVPECDAVSAS
                     SIDGPTARKNGGTDLLNQIHQKRMGVQLLGWVDSGIRFSLWSTKEPKFDGTGHIDIKG
                     FKVRGNPIYNAFLTNYLGAQIINLNSPELYTALERGTVELTAWTQIGTMDLNWDRYLK
                     YRILPDFFSTDLVILVNQKKWESLSPKSREILGRVAIAHESESLQALQALWTKEQAEL
                     EKRGLKTVSQSADASKRFVAGARAASLQRMKERMEKSGGTENYDRVVKLF"
     gene            1037..2278
                     /locus_tag="VGO02_02830"
     CDS             1037..2278
                     /locus_tag="VGO02_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEV7476651.1"
                     /translation="MLQTLAQKLVARAAGRAAVSPGEIVTCKVDLAMMHDSGGPRRVQ
                     PMLERLGAKVWDPSKVVLVTDHYLPEHDDESRKIVRIARDWARDNKVGGFYDAQGICH
                     VVLPERGHLRPGMFAVGGDSHSPTGGAFAAYMFGIGATEMLGVLVTGEIWLKVPHTIA
                     LEFSGRLSAGVSAKDVMLFLIGRLGMEGGQYQAVEYRGAAIRALPMQERMTLCNMAAE
                     LGAQTGLIASDEVTGEFLKKDVEIWDSDAGASLLAHHEFDASALPPQVAAPHSPANSR
                     PVNDFSSTEINLAYIGACTGAKLEDLRMAAQVFKGRKSKIRLLVAPASLRDQETAQKE
                     GVLKILQDAGAEILPNSCGICAGYGGHLLQDDAVAISSTARNFKGRMGSAKAQVYLAS
                     PYTVAASAIAGRIADPRELLP"
     gene            2275..>2652
                     /locus_tag="VGO02_02835"
     CDS             2275..>2652
                     /locus_tag="VGO02_02835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HEV7476652.1"
                     /translation="MKAWVLGDNVDTDVMAPGAYMKFGIEEIAKHCLESLLPSFVSTV
                     QKGDVVVAGRNFGTGSSREQAPAALKHLGVTALVAESFAGLFYRNAINLGLPAVACAQ
                     AKRIRQGDVLEVDFEKGKIRNVTT"
BASE COUNT          452 a          884 c          860 g          456 t
ORIGIN      
        1 tgaagagctt gaccaccctg tcgtagttct cggtgccgcc ggacttctcc atgcgctcct
       61 tcatgcgctg caggctggcg gcgcgcgcgc cggcaacaaa gcgcttggag gcgtccgcgg
      121 actgcgagac cgtcttcagg ccgcgcttct cgagctcggc ctgctccttc gtccacagcg
      181 cctgcagggc ctgcaggctc tcgctctcgt gcgcgatcgc cacgcggccc aggatctcgc
      241 ggctcttggg cgacagcgac tcccatttct tctggttgac caggatcacc aggtcggtcg
      301 agaagaagtc cggcaggatg cggtacttga ggtagcggtc ccagttgagg tccatggtgc
      361 cgatctgggt ccaggcggtg agctccaccg tgccgcgctc gagcgcggtg tagagctccg
      421 gcgagttgag gttgatgatc tgcgcgccga gatagttggt gaggaaagcg ttgtagatcg
      481 ggttgccgcg caccttgaag cccttgatgt cgatgtgccc ggtgccgtcg aacttgggct
      541 ccttggtcga ccagagcgag aagcggatgc cgctgtcgac ccagccgagg agctgcacgc
      601 ccatgcgctt ctggtggatc tggttaagga gatcggtgcc gccgttcttg cgcgcggtcg
      661 ggccgtcgat cgacgaggcc gagacggcgt cgcactcggg caccacggcg gcgtagaagg
      721 cgcaggccga gtacaccatg tcgaccacgc cgctcctcac cgcgccgggc tgctccatca
      781 tgccgatcgc ctcggggccg ccgcgcacct ggatggtgaa cacgcccttg ccggcttcgt
      841 tcgccttctt cacgaactcg aggaagctgc gcgaatagat gagcgacgcg gggaacacgt
      901 gcgtcgcgga gatggtgtcc tgcgcggccg ccggcagcgc gccggcgagc gccgcgagca
      961 cggcgatggt cttcagcatg cttttcatct aagcgggccc cctggtttgt ccctatgtct
     1021 taccattcta gcaaccatgc tccagacact ggcgcaaaag ctggtcgcgc gggcagccgg
     1081 tcgcgccgcc gtctcccccg gcgagatcgt cacctgcaag gtcgatctgg cgatgatgca
     1141 cgactcgggc gggccgcggc gcgtgcagcc gatgctcgag cgcctgggcg cgaaagtctg
     1201 ggatccgtcc aaagtggtgc tggtcaccga ccactatctg cccgagcacg acgacgaaag
     1261 ccgcaaaata gtgcgcatcg cacgcgactg ggcgcgcgac aacaaggtcg gcggcttcta
     1321 cgacgcgcag ggcatctgcc acgtggtgct gcccgagcgc ggccacctgc gccccggcat
     1381 gttcgccgtc ggcggcgaca gccactcccc caccggcggc gcattcgccg cctacatgtt
     1441 cggcatcggc gccaccgaga tgctcggcgt gctggtcacc ggcgagatct ggctcaaggt
     1501 gccgcatacc atcgccctgg agttctccgg ccggctctcg gccggcgtct cggccaagga
     1561 cgtgatgctg ttcctcatcg gccgcctggg catggaaggc ggccagtatc aggcggtgga
     1621 gtaccgcggc gccgcgatcc gcgccctgcc gatgcaggag cgcatgaccc tgtgcaacat
     1681 ggccgccgag ctcggcgcgc agaccggctt gatcgcctcc gacgaagtca ccggggaatt
     1741 tctgaagaag gatgtcgaga tctgggatag cgatgccggc gcctccctcc tcgcccatca
     1801 cgaattcgac gcttcggccc ttccacctca ggtggccgcg ccccatagcc ccgcgaatag
     1861 ccgacccgta aatgactttt caagtacaga aataaacctc gcctacatcg gcgcctgcac
     1921 cggcgcgaag ctcgaggatc tccggatggc agcccaggtc ttcaaaggcc gcaagtcgaa
     1981 gatccggctc ttggtcgcgc ccgccagcct gagggatcag gaaacagcgc agaaggaagg
     2041 cgtgctgaaa atcctgcagg acgccggcgc ggagatcctg cccaactcct gcggcatctg
     2101 cgcaggctac ggcgggcacc ttctgcagga cgatgcggtc gccatctcga gcaccgcgcg
     2161 caacttcaag gggcgcatgg gctcggcgaa ggcgcaggtc tacctcgcct ccccttacac
     2221 ggtcgctgcc tcggcgatcg ccgggcgcat cgcggatccc agggaactgc tgccatgaaa
     2281 gcctgggtgc tgggcgacaa cgtagacacc gacgtgatgg cacccggggc ctacatgaaa
     2341 ttcggcatcg aggagatcgc gaagcattgc ctggaatcgc tactaccttc tttcgtatcg
     2401 actgttcaga aaggcgatgt ggttgtggcc ggccgcaact tcggcaccgg ttcctcccgc
     2461 gagcaagcgc ccgccgccct gaagcacctg ggcgtgaccg ccctcgtcgc cgagtccttc
     2521 gccggcctgt tctaccgcaa cgcgatcaac ctcggcctgc ccgcggtcgc ctgcgcgcaa
     2581 gcgaaacgca tccgccaggg cgacgttctg gaagtggatt ttgaaaaggg aaaaatcagg
     2641 aacgtgacga cc
//
LOCUS       DASYJA010000134         1538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_658170, whole genome shotgun sequence.
ACCESSION   DASYJA010000134 DASYJA010000000
VERSION     DASYJA010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658170"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VGO02_02840"
     CDS             <1..529
                     /locus_tag="VGO02_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476653.1"
                     /translation="FSTKRSTSRTTERELVLDDQTIPAGTQLDTSSKTQFFVVNYQYS
                     LLRDDRVELSAMAGIYGARFKFGFDSSNPPRSIQSNTTAPLPMIGIGLDTFITPRWTV
                     STFIEGLALKVGDVRGSVTYIGMSTDYMLTRHFGLGLGVSAVHVGADVDKDSGAQHSF
                     DWRSNNFFAYGQLRF"
     gene            complement(545..>1538)
                     /locus_tag="VGO02_02845"
     CDS             complement(545..>1538)
                     /locus_tag="VGO02_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEV7476654.1"
                     /translation="AKARTPRATIDLLVISGGGDWGAFGAGVLKGWGRVKGELARPQF
                     DAVTGVSTGALIAPFAFLGDDESIERIVQLYRNPATDIATSRGLLFFLPNNPSFYLLP
                     GLERELRTALDRGMLERIAAQEASGRLLLVNTTNIDLGDLHPWDVVAEAKAALAAGEP
                     ERVHRILLASAGIPAIFPAQQIGPHLYVDGAITGNILYGARVDQEQGVAAQWRAKHGR
                     APAPRVRYWVIFNNQFRFPPQVTQARWPDIMSRATIMATQSATVSAMRHLEANAELTR
                     IKYGIDVEVRIMAVPEAWAPQVAGTFKKEVMNELADLGEKMGADPASWTKGVPD"
BASE COUNT          232 a          533 c          518 g          255 t
ORIGIN      
        1 cttcagcacg aagcgcagca ccagcaggac taccgagcgg gagctggtgc tcgacgacca
       61 gacgatcccg gccggcaccc agctggatac cagctccaag acgcaattct ttgtcgtgaa
      121 ctaccagtac tcgctgctgc gcgatgaccg cgtagagctc tccgcgatgg ccggcatcta
      181 cggcgcgcgc ttcaagttcg ggttcgactc cagcaacccg ccgaggagca tccagtccaa
      241 taccactgcg ccgctgccga tgatcggcat cggcctcgac acgttcatca cgccgcgctg
      301 gaccgtgagc acgttcatcg agggcctggc gctgaaggtc ggcgacgtca gggggagcgt
      361 cacctacatc ggcatgtcga ccgactacat gctgacacgg cacttcggcc tcggcttggg
      421 tgtcagcgcg gtgcacgtcg gcgccgacgt ggacaaggac agcggcgccc agcacagctt
      481 cgactggcgt tcgaacaact tcttcgccta cggacagttg cgcttctagc gccgagcgcc
      541 gtcgtcagtc cggtactcct ttagtccagc tcgccgggtc ggcgcccatc ttctcgccga
      601 ggtcggccag ctcgttcatc acttccttct tgaaggtgcc ggccacctgc ggtgcccagg
      661 cttcgggcac ggccatgatg cgcacctcca cgtcgatgcc gtacttgatg cgcgtcagct
      721 cggcgttcgc ctcgaggtgg cgcatcgcgc tcaccgtcgc gctctgcgtc gccatgatgg
      781 tggcgcgcga catgatgtcg ggccagcgcg cctgcgtcac ctgcggcggg aagcggaact
      841 ggttgttgaa gatcacccag tagcgcacgc gtggcgccgg cgcgcggccg tgcttcgcgc
      901 gccactgcgc ggcgaccccc tgctcctggt cgacgcgcgc gccgtacaga atgttgccgg
      961 tgattgcgcc gtcgacgtag aggtgcgggc cgatctgctg ggcggggaag atcgccggga
     1021 tgccggcgct cgccagcagg atgcggtgca cgcgctcggg ctccccggcg gcgagcgccg
     1081 ccttcgcttc ggccaccacg tcccaggggt gcaggtcgcc gaggtcgatg ttggtggtgt
     1141 tgaccagcag caggcgcccg ctcgcctcct gcgcggcgat gcgctcgagc atgccgcggt
     1201 cgagtgcggt gcgtagctcg cgctcgaggc cgggcagcag atagaacgac gggttgttcg
     1261 gcaggaagaa cagcagcccg cgcgacgtcg cgatgtcggt cgccgggttg cggtacagct
     1321 gcacgatgcg ctcgatcgac tcgtcgtcgc cgaggaaggc gaacggcgcg atcagcgcgc
     1381 cggtgctgac cccggtgacc gcgtcgaact gcggccgcgc caactcgccc tttacgcggc
     1441 cccagccctt gagcacgccg gcgccgaacg caccccagtc accgccgccc gagatgacca
     1501 gcaggtcgat ggttgcgcgc ggcgtgcgcg ctttcgcc
//
LOCUS       DASYJA010000135         1807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2655175, whole genome shotgun sequence.
ACCESSION   DASYJA010000135 DASYJA010000000
VERSION     DASYJA010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2655175"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1750
                     /locus_tag="VGO02_02850"
     CDS             <1..1750
                     /locus_tag="VGO02_02850"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEV7476655.1"
                     /translation="LPMYGVVFSFKDPFDTKDMRSTGGGDAAYDIDFPARDHVLVEQL
                     RNKGAIIFAKAVNTEYNGRAGDPGGRHKPDKVLPSTLGYQRATWGGNPSNPYDTTRAA
                     SLGSSSGSALSVSTNMVMASLGEETRASCRGPSNHNAVALILPHKAMLGFDGGAIGAD
                     IYCDRSGIHARTIADCAKVLDALKDPVEGYYDPRDPYTTVPRSSVLSTSYAEHVNSSA
                     ASGALKGMRIGVIRESMVYPKGSRTEEPIVTAAAKEIKSVLAGTLGAALVESRDPLWR
                     RDPDLEQMKTDFRVALAKLVPVFMPDLLFRLGRDGQPVFKEFAAAIVPTEFLPGRVFG
                     AGAMQPIDYCVAMAEGRIEPPSNLDLATVQQQELAMAFRFHLSQYLMRRAADWKMKGF
                     DETLANWAGLNARSKFWGEDQRAAFKNWEEVADPRNPLDGRQGVNERIMLRELLRRVD
                     MMVILENKLDALVRLHTPWPPGLIGHPHQYDIPSNLAPESLSGPNAGLTEVLIPAGYV
                     TTVYDPVFSLSADGKRYVSTPSSKPTTIPEPGLPFSLVFRAEPGREDVLLNIASAYEA
                     ASMRRIPPPAFGPVSL"
BASE COUNT          307 a          614 c          584 g          302 t
ORIGIN      
        1 gttgccgatg tacggcgtcg tgttctcgtt caaggacccg ttcgacacca aggacatgcg
       61 ctccaccggc ggcggcgacg cggcctacga catcgatttc ccggcgcgcg accatgtcct
      121 ggtcgagcag ctgcgcaaca agggcgccat catcttcgcc aaggcagtga acaccgaata
      181 caacgggcgc gccggcgatc ccggcggccg ccacaagcct gacaaggtgc tgccgtccac
      241 cctcggctat cagcgcgcca cctggggagg caatccgtcc aatccctacg acaccacgcg
      301 cgctgcttcg ctcggctcga gctccgggtc ggcgctctcg gtcagcacca acatggtgat
      361 ggccagcctc ggcgaggaga ctcgcgcctc ctgtcgcggc ccgtccaacc acaatgcggt
      421 ggccctgatc ctgccgcaca aggcgatgct gggcttcgac ggcggcgcga tcggagccga
      481 catctattgt gaccgcagcg gcatccatgc gcgcacgatc gctgactgcg ccaaggtcct
      541 cgatgcgctc aaggatccgg tggaaggcta ctacgacccg cgcgacccct atacgacggt
      601 gccgcgttca tcggtcctga gcacgtccta cgccgagcat gtgaactcgt cggcagcgtc
      661 gggcgcactg aagggcatgc gcatcggcgt gatccgcgag tccatggtct atccgaaggg
      721 ctccaggacc gaggagccga tcgtcacggc ggccgcgaag gagatcaaat cggtgctggc
      781 aggcacgctc ggcgccgcgc tggtcgagtc gcgcgatcca ctgtggcgac gcgatcccga
      841 cctcgagcag atgaagacgg atttccgcgt cgcgctggca aaactggtgc cggtgttcat
      901 gcccgacctg ctgttccggc tcggccgtga cggtcagccc gtcttcaagg agttcgcagc
      961 cgcgatcgta ccgaccgagt tcctgcccgg aagggtgttc ggcgccggcg ccatgcagcc
     1021 gatcgactat tgcgtggcga tggcggaggg ccggatcgaa ccgccgtcca atctcgacct
     1081 ggccaccgtc cagcagcagg agctggcgat ggccttccgg ttccaccttt cgcaatacct
     1141 gatgcggcgc gccgcggact ggaagatgaa aggcttcgac gagacgctgg caaactgggc
     1201 ggggctgaac gcgcggtcga aattctgggg cgaagatcag cgcgcggcgt tcaagaactg
     1261 ggaggaggtc gccgacccgc gcaatccact cgacggccgc cagggcgtca acgagcggat
     1321 catgctgcgc gagctcctgc gccgggtcga catgatggtg atcctggaga acaagctgga
     1381 tgcgctggtg cgcctgcaca cgccgtggcc gccgggtttg atcggtcacc cgcatcagta
     1441 cgacatcccc agcaacctcg ctcccgagtc gctcagcggg ccgaacgcgg ggctgaccga
     1501 ggtgctgatc ccggccggct acgtgacgac cgtctacgat ccggtgttct cgctttcggc
     1561 cgatggcaag cgctacgtat cgaccccgtc ctccaagccg accaccatcc cggagcccgg
     1621 tcttccattc tcgctggtgt tccgcgccga gccgggccgc gaggatgtgc tcctcaacat
     1681 cgcctcggcc tatgaggcgg cctcgatgcg ccggattccg ccgccggcgt tcggtccggt
     1741 gtcgctctag aaaggcgtcg agctcctgga gatcctgctt agcccaaagc cggttcttga
     1801 ctcatgg
//
LOCUS       DASYJA010000136         3160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2927852, whole genome shotgun sequence.
ACCESSION   DASYJA010000136 DASYJA010000000
VERSION     DASYJA010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2927852"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VGO02_02855"
     CDS             complement(<1..222)
                     /locus_tag="VGO02_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476656.1"
                     /translation="MKTNWTELLRHPTGGDHIVQTWQDEAFLAETVAEYVGSGLRGGE
                     GALIVARPAHFELFKRNLAEARAAGRSGQL"
     gene            complement(240..1034)
                     /locus_tag="VGO02_02860"
     CDS             complement(240..1034)
                     /locus_tag="VGO02_02860"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV7476657.1"
                     /translation="MKPPGSLANWRALDAQLGPDCLRVASYNVHGCVGRNNLRDVGRV
                     AAVIAELGCDTVGLQEVFGARALAQETQMFLVEGEPHKWHDRHVGNALLTRRKVLAVR
                     HHDWSWPDHEPRAALDVDLEVGGETVRIIVTHLGLKPYERRFQVRKILSLLKDTPIAE
                     RLVVLGDINEWLPLGRPLRWLNGLLGHSPAERSFPARWPLFALDRVWVRPRHALLAFG
                     AHRSPLAALASDHLPVKAIIATEEARSAKHDRAVGRPPTVRAVGSG"
     gene            complement(1031..>3160)
                     /locus_tag="VGO02_02865"
     CDS             complement(1031..>3160)
                     /locus_tag="VGO02_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEV7476658.1"
                     /translation="SLLRPGYNCWAVARASRAALLVDAEAYFKAFHDAALRAQCSIFV
                     LAWDFNSRTRLHFDAVPKGGAPALLGDFLNFLVRRRRGLHINVLNWDYPMVFGADREF
                     PPLYGFGWTPARRVHYRYDDTHPVAGCQHQKIVLIDDALAFVGGIDLTVRRWDTPEHR
                     ADEPRRVAYDKPYPPFHDLMMAVDGEAARKLAAIARERWKAATGKSLQPVAADGDPWP
                     EKLAPDLRDVEVGIARTMPPRGEVPAIREVEKLYLDTIAAARRTLYIENQYFTSPRIA
                     AALEKRLAERDGPEVVLVLRLLSHGWLEEHTMHVLRTRLVQRLLAADRFGRFHVYYPH
                     VPGLAEGCCLDVHSKLMIADDSLLRVGSANLCNRSMALDSECDVVIESRGRAEVAETI
                     TGLRDRLLGEHLDAQPHAVGAAVARAGTLHGAIAALGRSERTLRPFEDLKEWPEAIVE
                     LAAVADPEEPIAPAILDLERKSEGEEDRGAPGAAWLKLGLIGALFLGLAALWRFTPLA
                     HVVTAENVIAWAHDFGSRWWAPLVVIAAYTPACFVMFPRPLITLAAVVAFGPWLGFLY
                     ALIGIVTSAAVTYHVGRRMRRDSVRRLAGPKLDRIVAVLKKHGLVAMTLLRLVPLAPF
                     AVEGMVAGAVRLKLWHLLVGTAIGMLPGTLAATLFGDQIETALSGGGINWWVIGAALA
                     LLGGGAIAVKRWFGRMSARISEQLPGK"
BASE COUNT          481 a         1075 c         1164 g          440 t
ORIGIN      
        1 cagctgcccg ctgcggcccg cggcgcgggc ctccgccagg ttgcgcttga acagctcgaa
       61 gtgtgcgggc cgcgcgacga tcagggcgcc ttcgccaccg cgcaggccgc tgccgacgta
      121 ttcggccacc gtttcagcga ggaacgcctc gtcctgccag gtctggacga tatggtcgcc
      181 gccggtgggg tgccgcagaa gctcggtcca gttcgttttc atttttcttg gcgccgattc
      241 tagcccgacc ccacggcgcg cacagtcggc gggcgcccga cagccctgtc gtgttttgca
      301 ctacgcgcct cctcggtcgc gatgatcgcc ttgaccggca gatggtccga ggcgagcgcc
      361 gcgagcggcg agcgatgggc gccgaaagcg agcagggcgt ggcgcggccg cacccagacg
      421 cggtcgagcg cgaacagcgg ccagcgcgcc gggaacgagc gctcggccgg cgaatggccg
      481 agcaggccgt tcaaccagcg cagcggccgg ccgagcggca gccactcgtt gatgtcgccg
      541 aggacgacca ggcgctccgc gatcggggtg tctttcaaca atgacaatat tttcctgacc
      601 tgaaagcgtc gctcgtaggg cttgaggccg agatgggtga cgatgatgcg caccgtctcg
      661 ccgccgactt cgaggtcgac gtcgagcgcc gcacgcggct cgtggtcggg ccagctccag
      721 tcgtggtggc gcacggcgag cacttttctg cgggtgagca gggcattccc cacgtgccgg
      781 tcgtgccact tatggggttc gccttccacc agaaacatct gcgtttcctg ggcgagggcc
      841 cgtgcgccaa aaacctcctg caatcccacg gtgtcgcagc ccagctcggc gatcaccgcc
      901 gccacgcgcc cgacgtcgcg caggttgttg cggccgacgc agccgtgcac gttgtaggag
      961 gcgacgcgca ggcagtccgg gccgagctgc gcgtcgaggg cacgccagtt ggcaaggctg
     1021 cccgggggct tcacttgccc ggaagctgct ccgagatgcg cgcggacatg cggccgaacc
     1081 agcgcttcac cgcgatggca ccgccgccga gcagcgcgag cgcggcgccg atgacccacc
     1141 agttgatgcc gccgccggac agcgcggtct cgatctggtc gccgaacagc gtcgccgcga
     1201 gcgtgccggg cagcatgccg atggccgtgc ccaccagcag gtgccacagc ttgaggcgca
     1261 ccgcgccggc gaccatgcct tcgaccgcga agggcgcgag gggcaccagg cgcagcagcg
     1321 tcatcgccac caggccgtgc ttcttcagca ccgcaacgat gcggtcgagc ttcgggccgg
     1381 cgaggcggcg cacgctatcg cggcgcatgc ggcggccgac gtggtaggtg actgcggcgg
     1441 aggtgacgat gccgatcagg gcatacagaa agccgagcca ggggccgaag gcgaccacag
     1501 ccgcgagggt gatcagcggg cgcgggaaca tgacgaagca ggccggcgtg tacgcggcga
     1561 tgaccaccag cggcgcccac cagcgcgagc cgaaatcgtg cgcccaggca atgacgttct
     1621 cggcggtgac gacgtgcgcg agcggcgtga agcgccacag cgcggcgagg cccaggaaca
     1681 gcgcgccgat caggccgagc ttgagccagg cggcgcccgg cgcgccgcgg tcttcctcgc
     1741 cttcggactt gcgctccagg tcgaggatcg ccggcgcgat cggctcctcg ggatcggcca
     1801 ccgcggcgag ctcgacgatg gcctcgggcc attccttgag gtcctcgaac gggcgcaggg
     1861 tacgctcgct ccggcccagc gcggcgatgg cgccgtgcag ggttccggcg cgcgccacgg
     1921 cggcgcccac ggcatgcggc tgcgcgtcga ggtgctcgcc gagcagccgg tcgcgcaggc
     1981 cggtgatggt ttccgccacc tcggcgcggc cgcgcgactc gatcaccacg tcgcactcgg
     2041 agtcgagcgc catcgagcgg ttgcacaggt tggccgagcc gacgcgcagc aagctgtcgt
     2101 cggcaatcat cagcttggag tgcacatcga ggcagcagcc ctctgccagg ccgggcacgt
     2161 gcgggtagta cacgtggaag cgaccgaacc ggtcggcggc gagcaggcgc tgcaccaggc
     2221 gcgtgcgcag cacgtgcatg gtgtgctcct cgagccagcc gtgagaaagc aggcgcagca
     2281 ccagcacgac ctccggaccg tcgcgctcgg cgaggcgctt ctcgagcgcc gcagcgatgc
     2341 gcggcgaggt gaaatactgg ttctcgatgt agagcgtgcg gcgcgccgcg gcgatggtgt
     2401 cgaggtacag cttctcgacc tcgcgtatcg ccggcacctc gccgcgcggc ggcatggtgc
     2461 gcgcgatgcc gacctcgacg tcgcgcaggt cgggcgcgag cttctcgggc caggggtcgc
     2521 cgtcggcggc gaccggctgc aggctcttgc cggtagcggc cttccagcgc tcgcgggcga
     2581 tggccgcgag cttgcgcgcc gcctcgccgt ccaccgccat catcaggtcg tggaacggcg
     2641 ggtagggctt gtcgtaggcg acgcggcgcg gctcgtctgc gcggtgctcg ggcgtgtccc
     2701 agcgccgcac ggtgaggtcg atgccgccga cgaaggcgag ggcgtcgtcg atgaggacga
     2761 tcttctggtg ctggcagccg gcgaccggat gcgtatcgtc gtagcgatag tgcacgcgcc
     2821 gcgccggcgt ccagccgaag ccgtatagcg gcgggaattc ccggtcggcg ccgaacacca
     2881 tcgggtagtc ccagttcagc acgttgatgt gcagcccgcg gcggcggcgc acgaggaaat
     2941 tgaggaagtc gccgagcagc gccggcgcgc cgcccttcgg cacggcgtcg aaatgcagcc
     3001 gcgtgcggct gttgaagtcc caggcgagga cgaagatcga gcactgcgcg cgcagcgctg
     3061 cgtcgtgaaa cgccttgaaa taggcctcgg cgtcgactag cagcgcggcg cggctggcgc
     3121 gcgccaccgc ccagcagttg tagccggggc ggaggaggct
//
LOCUS       DASYJA010000137         2962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2474074, whole genome shotgun sequence.
ACCESSION   DASYJA010000137 DASYJA010000000
VERSION     DASYJA010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2474074"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="VGO02_02870"
     CDS             complement(<1..1118)
                     /locus_tag="VGO02_02870"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7476659.1"
                     /translation="MHDLVIRNALLLDGMGSPPVVADLAATGGQIVEIGKVKEKGKQT
                     IDADGLALMPGIIDNHTHYDAQITWDPLVAPSPALGVTTAVIGNCGFTIAPCRPADRE
                     LVMRNLTQVEGMSLEVLRAGIRWDFESLPQYLDMLERRGAALNVAAFAGHSAIRTYVM
                     GEAATQRAATDHEIDLMKKHVLQGLQAGAVGFSTSTSPAHNGEGGLPMPSRLADEREM
                     RALAGCLKDAGRGVFMLTKGGHTPIAFLEQLAADTGRPVVIAALLHNSTNPESVFKDL
                     DAIEDANQRGHKLLGAVSCCPLSMDFTLHSPYVIEGLEAWQPALPLKGEAFRKIISSP
                     EFRKALRAELSRPAHFRLFNGEWDKVEVIESRNKSFEQ"
     gene            1141..1917
                     /locus_tag="VGO02_02875"
     CDS             1141..1917
                     /locus_tag="VGO02_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011295807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7476660.1"
                     /translation="MKLFELPGKAAIVTGAGRGIGKATAELFAAAGAAVAVIDVSEEH
                     AQRTAEAIGSAGGTAIPIVCDVTGEKAVEAAFAQAADELGGIDILVNNAGMAIRKPAT
                     ELPLADWQKVIDLNLTALFLCSRVAARHMRKARRDAPSGGAIVNLASIMGLSGGLFPN
                     SSYQASKGGVVNLTRALALEWAADRIRVNAVAPTFVNTDFTAQIFNNPDVLEKVEAHT
                     PLGVLPEVEDIAAAILFLASPAARCVTGIVLPVDSGYLAR"
     gene            complement(1930..2880)
                     /locus_tag="VGO02_02880"
     CDS             complement(1930..2880)
                     /locus_tag="VGO02_02880"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEV7476661.1"
                     /translation="MLRFIAALAACSLPLAAHASCGSAFCMVNTGWSAQGAWTEPGTR
                     FDLRYEYINQDQPLAGKDKVSVGQIPQHHDEVSTKNQNLVATLDQGFGANWGVNVSLP
                     VVSREHDHIHNHMGEQIPEHWEFTALGDMRVLGRYQFDPRQRGDMHDVWGLNFGLKLP
                     TGKFDERNADGDLAERTLQPGSGTTDAMLGAYFSRHLPLKDLSWFAQALYQAPLNERA
                     DFKPGARFGLDLGTRYDMGERVGLMLQLNYLQKARDKGAEAEPDDSGGTFLFVSPGAS
                     WNLTKDFQIYGFVQVPVYQKVNGVQLVADYALVVGLSTRF"
BASE COUNT          477 a          965 c         1023 g          497 t
ORIGIN      
        1 ttctgctcga agctcttgtt cctcgactcg atgacttcca ccttgtccca ttccccgttg
       61 aacaggcgga aatgcgcggg cctcgaaagc tcggcacgca gagcctttct gaattccggg
      121 gaagaaatga tttttcgaaa agcctccccc ttcaaaggca atgcgggctg ccaggcctcc
      181 aggccttcta ttacgtaagg cgaatgcagc gtgaaatcca tcgatagcgg acagcacgac
      241 acggcgccga gcagcttgtg cccgcgctgg ttggcatcct cgatggcgtc gaggtccttg
      301 aataccgact cggggttggt gctgttgtgg agcagggcgg cgatgaccac cggccggccg
      361 gtgtcggcgg cgagctgctc gaggaacgca atcggcgtgt ggccaccctt ggtcagcatg
      421 aagacgccgc gcccggcgtc cttcaggcag ccggcgagcg cgcgcatctc gcgctcgtcg
      481 gcgaggcgcg agggcatcgg caggccgccc tcgccgttgt gcgccggcga ggtgctggtc
      541 gagaagccga ccgcgccggc ctgcagcccc tggagaacgt gttttttcat cagatcgatc
      601 tcatgatccg tcgcagcgcg ctgggtggcc gcttcgccca tgacgtaggt gcggatcgcc
      661 gagtggccgg cgaaggcggc gacgttcagc gccgcgccgc ggcgctcgag catgtcgagg
      721 tattgcggca gcgactcgaa atcccagcgg atgccggcgc gcagcacctc gagcgacatt
      781 ccctcgacct gggtcaggtt gcgcatcacg agctcccggt ccgcggggcg gcagggcgcg
      841 atggtgaagc cgcagttgcc gattaccgca gtagtaacgc cgagcgcggg cgagggtgcg
      901 acgagcgggt cccaggtgat ctgggcgtcg tagtgggtat ggttgtcgat gatccccggc
      961 atcagcgcca gcccgtcggc atcgatggtt tgctttcctt tctccttcac cttcccgatc
     1021 tcgacgattt gccctccggt cgctgccagg tctgcgacca cgggagggga acccattccg
     1081 tcgagcagga gcgcgttgcg gatgacgagg tcgtgcatgg ccggtcagta tacttagcgc
     1141 atgaagctat tcgagctgcc gggcaaggcg gccattgtca cgggagcggg tcgggggatc
     1201 ggcaaggcaa ccgccgagct gttcgccgcc gccggcgcgg cggtggcggt gatcgacgtg
     1261 agcgaggagc acgcgcagcg caccgccgag gcgatcggca gcgccggcgg cacggcgatc
     1321 ccgatcgtct gcgatgtgac cggcgagaag gcggtcgagg cggcgttcgc gcaagccgcc
     1381 gacgagctcg gcggcatcga catcctggtc aataacgccg gcatggcgat caggaagccg
     1441 gccaccgagc tgccgctcgc cgactggcag aaggtgatcg acctcaacct gaccgcgctg
     1501 ttcctgtgct cgcgcgtcgc cgcgcgccac atgcgcaaag cgcggcgcga cgcgccctcc
     1561 ggcggcgcca tcgtcaacct ggcctcgatc atgggcctgt cgggcggcct gttcccgaac
     1621 tcgtcctacc aggcctcgaa gggcggcgtg gtcaacctga cgcgcgccct cgccctggag
     1681 tgggcagccg accgcatccg cgtcaacgcc gtggcgccga ccttcgtcaa caccgacttc
     1741 accgcgcaga tcttcaacaa ccccgacgtg ctggagaagg tcgaggcgca cacgccgctc
     1801 ggcgtgctgc ccgaggtcga ggacatcgcc gccgcgatcc tgttcctcgc cagcccggcg
     1861 gcgcgctgcg tcaccggcat cgtgctgccg gtggacagcg gctatttggc caggtagagg
     1921 caggccccgc tagaagcgcg tgctcaggcc gaccaccagc gcgtagtcgg cgaccagctg
     1981 cacgccgttc accttctggt agaccggcac ctgcacgaag ccgtagatct ggaagtcctt
     2041 ggtgagattc cagctggcgc ccgggctgac gaagaggaac gtgccgccgc tgtcgtcggg
     2101 ctccgcctcg gcgcctttgt cgcgcgcctt ctgcagatag ttgagctgca gcatcaagcc
     2161 gacgcgctca cccatgtcgt agcgcgtgcc caggtccagg ccgaaccggg cgcccggctt
     2221 gaagtccgct cgctcgttca acggcgcctg atagagggcc tgggcgaacc acgagaggtc
     2281 cttgagcggc agatggcggg agaagtacgc cccgagcatg gcatcggtcg tgccgctgcc
     2341 cggctgcagc gtgcgctcgg caaggtcgcc gtcggcgttg cgctcgtcga acttgccggt
     2401 cggcagcttg aggccgaaat tgaggcccca gacgtcgtgc atgtcgccgc gctggcgtgg
     2461 atcgaactgg tagcgcccga gcacccgcat gtctcccagc gcggtgaact cccagtgctc
     2521 agggatctgc tcgcccatat gattgtggat gtggtcgtgc tcgcggctta cgaccggcag
     2581 cgacacgttg accccccaat tggcgccgaa gccttggtcc agggtggcca ccaggttctg
     2641 gttcttggtg gatacctcgt cgtggtgctg cggaatctgg cccaccgaga ccttgtcctt
     2701 gccggcgagc ggctggtcct ggttgatgta ctcgtagcgc aggtcgaagc gcgtgcccgg
     2761 ctcggtccag gcgccctggg cgctccagcc ggtgttgacc atgcagaacg ccgagccgca
     2821 cgaagcgtgc gcggcaagcg gaagagaaca cgcggcaagc gccgcgatga aacgaagcat
     2881 aagaaccccc tgtttctggt tagaaaaaaa gcggtccggc gctcaggcgc cggcaggggg
     2941 gccgcgggga ggggctgcgg gg
//
LOCUS       DASYJA010000138         2714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1384700, whole genome shotgun sequence.
ACCESSION   DASYJA010000138 DASYJA010000000
VERSION     DASYJA010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384700"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VGO02_02885"
     CDS             complement(<1..713)
                     /locus_tag="VGO02_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEV7476662.1"
                     /translation="MKPDHPVTFKPETAADAWLGLLAARGVEYLFANGGTDFAPVVEA
                     YAKGQSLGWRLPEVVIVPHENMGVAMAHGYAMVTGRPQAMMVHVGVGTANALNGLINA
                     SRQNVPILFTAGRTPFTESGALPAARNNYIHWAQEHFDQGGMLREFMKWDYELKHAEQ
                     VETVIDRALAIAKSEPQGPVYVTLPREILASAFTSQFSANSTIAPASPPGADQEVLEE
                     VAKLLGRARMPLLITANGGR"
     gene            complement(710..1411)
                     /locus_tag="VGO02_02890"
     CDS             complement(710..1411)
                     /locus_tag="VGO02_02890"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEV7476663.1"
                     /translation="MYEELIREVADLHGDLVRNIQTIRVSQDLFDDLSHDAAAQAVAI
                     AAEAAGRIPSEAPLITRPFDYGTVITFPFANFNGHATRFSDGLRYGVWYGSLELETTV
                     HETVYHRHRFLQDAFAGEDRAIRSERRVFDVRCDAILLDLRGKEAAEPGLLDRRSYAF
                     TQPLGAYLQAQGSNGLLVRSARCQGMNAAIFRAEALSRVRDLCHLTYIMNPTQDTAAI
                     ERTPGETWLRINIPL"
     gene            complement(1412..1813)
                     /locus_tag="VGO02_02895"
     CDS             complement(1412..1813)
                     /locus_tag="VGO02_02895"
                     /inference="COORDINATES: protein motif:HMM:NF042861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbcA/ParS/Xre antitoxin family protein"
                     /protein_id="HEV7476664.1"
                     /translation="MPASTQPALPDLRARENRAQLARMIVQLLDHWQLGAADQAVLLG
                     LSGQSRSTLARYRRGEPLADSADLLARAGHLLGIHKALRILFPHDRDLAYRWVSAPNR
                     RFGERAPLELMKQGFEGLLAVRRYLDFERGR"
     gene            1899..>2714
                     /locus_tag="VGO02_02900"
     CDS             1899..>2714
                     /locus_tag="VGO02_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEV7476665.1"
                     /translation="MTCVAQLVEMFGSQADVARAFQLDRAVVHNWVKTGYVPARWAGE
                     VERVTQGRIHAVDVINEASVKNPVRVKSRPDDAPFGLSESDPMSQYAPAKRIQSFHPP
                     QRTLMGPGPTEIHPRVLTTMSQPAIGYLDPVFVEMMEELKSLLRYVYQTKNALTFPLS
                     GPGSVGMEYCFVNMVAPGDKVIVCQNGVFGGRMLENVIRMGGTPVLVEDKWGEPVDPQ
                     KVEDALKKNKDSKIVAFVHAETSTGCQSDAKTLTQLAHKYGAMVIVDAVTSLAG"
BASE COUNT          469 a          860 c          937 g          448 t
ORIGIN      
        1 cgaccgccgt ttgccgtgat cagcaatggc atccgggccc gacccagcag ctttgccact
       61 tcttccagga cctcctgatc ggcgcccgga ggggaggcgg gagcgatggt tgagttggca
      121 ctgaactgag aggtgaaagc ggaagccagg atttcgcgag gcaatgtcac gtatacggga
      181 ccctgcggct cgctcttcgc gatcgcgagg gcgcggtcga tcacggtctc gacctgctcg
      241 gcgtgcttca gctcgtagtc ccacttcatg aattcgcgca gcatgccgcc ctggtcgaaa
      301 tgctcctgcg cccagtggat gtagttgttg cgagcggcgg gtagcgcgcc cgactcggtg
      361 aacggcgtgc ggccggcggt gaagaggatc ggcacgttct ggcgcgatgc gttgatgagg
      421 ccgttcagcg cgttcgccgt gccgacgccg acgtgcacca tcatcgcctg cggccggccg
      481 gtgaccatgg cgtagccgtg cgccatcgcc acgcccatgt tctcgtgcgg cacgatcacc
      541 acctcgggca ggcgccagcc gaggctctgg cccttggcgt aggcctccac caccggcgcg
      601 aagtcggtgc cgccgttggc aaaaaggtat tcgacaccgc gcgccgcgag caggccgagc
      661 caggcgtcag ccgcggtttc cggtttgaag gtgacggggt gatcgggctt catagaggaa
      721 tattaatcct tagccaagtc tcgccagggg tgcgttcgat cgcagccgta tcctgcgtcg
      781 gattcatgat gtaggtcaga tggcaaaggt cgcgcacgcg ggaaagggct tcggcgcgga
      841 agatggcggc gttcatgcct tggcagcgcg ccgacctgac cagcaagccg ttgctgccct
      901 gggcctgcag gtacgcgccc agcggctgcg tgaaagcgta gctccggcgg tccagcaggc
      961 cgggttcggc tgcttccttg ccgcgcaggt cgagcaggat cgcgtcgcag cgcacgtcga
     1021 atacacgccg ctcgctgcgg atcgcccggt cctcgccggc gaaggcatcc tgcaggaagc
     1081 gatggcggtg atagaccgtc tcgtggacgg tggtctccag ctcgagggag ccgtaccaca
     1141 cgccatagcg cagcccgtct gagaagcgcg tggcgtggcc gttgaagttg gcgaacggga
     1201 aggtgatcac cgtgccgtag tcgaagggcc gcgtgatgag cggggcttcg ctcgggatgc
     1261 ggccggcggc ctcggcggcg atggccaccg cctgcgcagc ggcatcgtgc gaaagatcgt
     1321 cgaacaaatc ctgcgagacg cggatcgtct ggatgtttcg caccagatcg ccgtgaaggt
     1381 cggcgacctc gcggatcagc tcctcataca cctaccgacc ccgttcgaag tccaggtagc
     1441 ggcgcaccgc gagcaggccc tcgaagccct gcttcatgag ctcgagcggc gcgcgctcgc
     1501 cgaagcggcg gttcggcgcg ctcacccagc gatacgcgag gtcgcggtcg tgcggaaaca
     1561 ggatgcgcag cgccttatgg atgccgagca ggtgcccggc gcgcgccagc aggtccgcgc
     1621 tgtcggcgag cggctcgccg cggcggtagc gggcgagcgt cgagcgcgac tgcccggaca
     1681 atcccagaag gacggcctgg tcggctgcgc cgagttgcca gtggtcgagc agctgcacga
     1741 tcattcgcgc gagttgcgct cggttttccc gggctcgcag gtccgggagg gcaggctggg
     1801 ttgaggcagg catgtaccag atgatacgac atatgtagtg ataggtacat caagacggag
     1861 gtttgtcaag ccgagtttga cagcctaaaa tcggcgccgt gacctgcgtt gcgcaacttg
     1921 tcgagatgtt cggtagccag gcggacgtcg cgcgcgcctt ccagctcgac cgcgcggtgg
     1981 tgcacaactg ggtgaagacc ggctacgtgc cggcgcgctg ggccggcgag gtcgagcggg
     2041 tcactcaggg ccgcatccac gcggtcgacg taatcaacga agcaagcgta aaaaacccgg
     2101 tgcgcgtgaa gtcgcgcccc gatgacgccc cgttcggcct ttccgaaagc gatcccatga
     2161 gccagtacgc acctgcgaag cgcatccagt ccttccatcc accccagcgc accctgatgg
     2221 gtcccggccc gaccgagatc cacccgcgcg tgctcaccac catgagccag ccggcgatcg
     2281 gctacctcga cccggtgttc gtcgagatga tggaggagct caaatcgctt ctgcgctacg
     2341 tgtaccagac gaagaacgcg ctcaccttcc cgctctccgg gccgggctcg gtcggcatgg
     2401 agtactgctt cgtcaacatg gtggcgccgg gcgacaaggt catcgtctgc cagaacggcg
     2461 tgttcggcgg ccgcatgctg gagaacgtga tccgcatggg cggcacgccg gtgctggtcg
     2521 aggacaagtg gggcgagccg gtcgatccgc agaaggtcga ggacgcgctg aagaagaaca
     2581 aggactcgaa gatcgtcgcc ttcgtgcacg ccgagacctc gaccggctgc cagtcggacg
     2641 cgaagaccct gacgcagctc gcccacaagt acggcgcgat ggtgatcgtc gacgccgtca
     2701 cctcgctcgc cggc
//
LOCUS       DASYJA010000139         2839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_567687, whole genome shotgun sequence.
ACCESSION   DASYJA010000139 DASYJA010000000
VERSION     DASYJA010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567687"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VGO02_02905"
     CDS             complement(<1..344)
                     /locus_tag="VGO02_02905"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEV7476666.1"
                     /translation="MHTYKTLTLGEAHTILDAAQKKAEELGIPEVLCVADNAGYPIAL
                     RRLDGGKVTSVQIAMNKAFTAAGHRKPTHNYKNALPGEEAFGIFTQHEGRFTVFVGGF
                     PIFVDGQCVGSVA"
     gene            complement(360..1091)
                     /locus_tag="VGO02_02910"
     CDS             complement(360..1091)
                     /locus_tag="VGO02_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEV7476667.1"
                     /translation="MAKSAFRLALEEAIEQRHSAVHPWSEAWTSGKLDRRLLGEWVKQ
                     HFHYVSHFSEWLGAVYAACPHWDVQHFLLENITEEEGFVGSGDFAPVRHSELLLDFAE
                     TCGMKRDEILNAQVKGELLPETLGLQSWCAVQAQKPFVEALSGLLIGLESQVPRIYSK
                     TTPPLLEKYGFAEDEVTFFTLHIVADAEHGEKGFEIVEKYATTDEQRAACVRLVKEAT
                     MMRRLYLDGLYRTFVAQAGTAKLAA"
     gene            1200..2012
                     /locus_tag="VGO02_02915"
     CDS             1200..2012
                     /locus_tag="VGO02_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEV7476668.1"
                     /translation="MKSEDYAQGDAFQIEKRTLFSSEWLPLCADGQIAKAGDFLSATV
                     GGWGVFGARDKEGTVRVLRNACRHQNMQVVGTPSGNCESFRCRFHGWTYDLQGRFLGA
                     PPPVAPKDPQAPDLHLASLATSSASGLVFFSLAASAAPPDLGGPLPAYGGTLTTEIAC
                     NWKVCVEHLLAEHTPSPEFGWHWPLLAVRRAGPVTIMEQVVPHTFLRTRLLTHVFGGA
                     ADEHKQADATIKHVCERLQADRNEGKPAADGPLVATFHRQLADAYAQDRTTT"
     gene            2156..>2839
                     /locus_tag="VGO02_02920"
     CDS             2156..>2839
                     /locus_tag="VGO02_02920"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HEV7476669.1"
                     /translation="MSLQANVVARLPHVEADMNYLAPMADRPRNYTFEPPAGVPRSNA
                     NHEAHKVAIHDARPVASEVSLDANGFALVNHRSAVRDFFDDEEVRRVYYPEVERVLKQ
                     ATGAYRVHIFDHTVRRRVNGAQDRSDAPRQPVQRVHIDHTARSGPQRVRDLLPDEADE
                     LLKGRVQVINLWRPINGPLQDAPLAVCDATTIRADDLVPSDLVYQHRVGETYSVKYNP
                     AHQWFYVPRM"
BASE COUNT          479 a          968 c          906 g          486 t
ORIGIN      
        1 gcgaccgagc cgacgcactg gccgtcgacg aagatcggga agccgccgac gaacaccgtg
       61 aagcggccct cgtgctgggt gaagatgccg aaggcctcct cgccgggcag cgcgttcttg
      121 tagttgtggg tgggcttgcg atggccggcc gcggtgaagg ccttgttcat ggcgatctgc
      181 acgctggtca ccttgccgcc gtcgagccgg cgcagcgcga tcggatagcc ggcattgtcg
      241 gcgacgcaga ggacctcggg aatgccgagc tcctccgcct tcttttgcgc cgcgtcgagg
      301 atggtgtggg cctcgccgag cgtcagggtc ttgtaggtgt gcacagccca ggatcccgac
      361 tatgccgcga gcttcgcggt cccggcctgg gcgacgaagg tgcggtagag gccgtcgaga
      421 tagagccggc gcatcatcgt cgcttccttg accaggcgca cgcaggcggc gcgttgctcg
      481 tccgtggtgg cgtacttctc gacgatctcg aagcccttct cgccgtgctc ggcgtcggcc
      541 acgatgtgca gggtgaagaa ggtgacctcg tcctcggcga agccgtattt ctcgaggagc
      601 ggcggcgtgg tcttgctgta gatccgcggc acctggctct ccagcccgat cagcaggccc
      661 gacagcgcct cgacgaaggg cttctgcgcc tgcacggcgc accagctctg caggccgagc
      721 gtctcgggca gcagctcgcc cttcacctgg gcattgagga tctcgtcgcg cttcatgccg
      781 caggtctcgg cgaagtcgag cagcagctcg gagtggcgga ccggcgcgaa gtcgcccgaa
      841 ccgacgaagc cttcttcctc ggtgatgttc tcgagcagga agtgctgcac gtcccaatgc
      901 gggcaggcgg cgtagaccgc gcccagccat tccgagaagt ggctgacata gtggaaatgc
      961 tgcttcaccc actcgccgag caggcggcgg tcgagcttgc ccgacgtcca ggcctcgctc
     1021 cacggatgca cggccgaatg acgctgttcg atcgcttcct cgagagccag acggaacgca
     1081 cttttggcca tggcgagcct ccttggttgg gacgcgaaaa ttgtatacaa tttgagtcga
     1141 tttcgtcaat atagggcccc ttcctgggac gcataaattc ggatccgtat acaaatccca
     1201 tgaaatccga ggattacgct caaggcgacg ccttccagat cgaaaaacgc acgttgttct
     1261 cgagcgaatg gctgccgctc tgcgccgacg ggcagatcgc caaggccggc gacttcctgt
     1321 cggccaccgt cggcggctgg ggcgtcttcg gcgcccgcga caaggaaggg accgtgcggg
     1381 tcctgcgcaa tgcctgccgg caccagaaca tgcaggtcgt aggcacgccg tcaggcaatt
     1441 gcgagagctt ccgctgccgc ttccacggct ggacctacga cctgcagggc aggtttttgg
     1501 gcgcgccgcc gccggttgct cccaaggatc cgcaggcgcc cgatcttcac ctggcatcgc
     1561 tcgccacgag cagcgcctcg ggcttggtct tcttcagcct cgccgcctcc gccgcgccgc
     1621 ccgatctcgg cggcccgctg ccggcctatg gcggcacact cacgaccgag atcgcctgca
     1681 actggaaggt ctgcgtcgag catctgctgg ccgagcacac gccgtcgccg gagttcggct
     1741 ggcactggcc gctgctcgcc gtgcggcgtg ccggcccggt gacgatcatg gagcaggtcg
     1801 tgccgcacac cttcctgcgc acgcggcttc tcacccatgt gttcggcggt gccgccgacg
     1861 aacacaagca ggctgacgcc acgatcaagc atgtttgcga gcgtctccag gccgatcgca
     1921 atgaaggcaa gcccgcagcc gacgggccgc tggtggcgac gttccaccgc cagcttgccg
     1981 acgcttacgc gcaggaccgc acgacgacct gaatctgctg ccgttctcgc aagcggcaga
     2041 agatttttcg cttcgcatta cgtccactca tgaggcgcac atttcttctc catggcgcgc
     2101 ggtgccgcct gacccgacat cgaaaagaag caattccagt ccggaggtca tgtccatgag
     2161 cctgcaagcc aacgtcgtcg cccgcctgcc gcacgtcgaa gccgacatga actacctcgc
     2221 gccgatggcc gatcggccgc gcaactacac cttcgagccg ccggccggcg tgccgcgcag
     2281 caacgccaac cacgaggcgc acaaggtcgc gatccatgat gcgcgtccgg tggccagcga
     2341 ggtgtcgctc gacgccaacg gctttgccct ggtgaaccac aggagcgcgg tgcgtgattt
     2401 cttcgacgac gaggaagtac gccgcgtcta ctacccggaa gtcgagcgcg tcctgaagca
     2461 ggcgacgggc gcctaccgag tccacatctt cgatcacacc gtccgccgcc gcgtcaacgg
     2521 cgcgcaggat cgcagcgatg cgccgcgcca gccggtgcag cgcgtccata tcgatcacac
     2581 cgcccgctcg ggtccgcagc gcgtgcgcga cctgctgccc gacgaggccg acgagctgct
     2641 gaagggccgc gtccaggtca tcaacctgtg gcgtcccatc aacgggccac tgcaggacgc
     2701 gccgctcgcc gtgtgcgacg ccaccaccat ccgcgccgac gatctggtgc cctcggacct
     2761 cgtctatcag cacagggtcg gcgagaccta ttcggtgaag tacaatcccg cgcaccaatg
     2821 gttctacgtg ccgcgcatg
//
LOCUS       DASYJA010000140         1688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_567754, whole genome shotgun sequence.
ACCESSION   DASYJA010000140 DASYJA010000000
VERSION     DASYJA010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567754"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VGO02_02925"
     CDS             <1..352
                     /locus_tag="VGO02_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7476670.1"
                     /translation="GGKKGGVLTVDFTVLGIPCLGLNGGPVFKHNEAFSFQIATEDQA
                     ETDRYWNAIVGNGGQESQCGWCKDKWGISWQITPRVLTDALAAGGDEAKRAFAAMMEM
                     GKIDVAAIEAARRG"
     gene            complement(379..603)
                     /locus_tag="VGO02_02930"
     CDS             complement(379..603)
                     /locus_tag="VGO02_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476671.1"
                     /translation="MAVAALKQRPAPALKQRPAPAPRTFHATMLVTRAEEWCVEAETA
                     DEARELLAAGQGHRCHVGERVQFEVERVED"
     gene            777..1649
                     /locus_tag="VGO02_02935"
     CDS             777..1649
                     /locus_tag="VGO02_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEV7476672.1"
                     /translation="MAPRAYWKGYLKLSLVSCPIALFPATSEREKISFHQLNKSTGNR
                     IKYRKVDADTGDEVEAPDIIKGYEVGKGEYLELEPEELEAIAIESKRTIEIDEFVPRK
                     EIDELYLNSPYYIVPDGEVGQQAFAVIREAIRQEGMVAIGKVVFTSREHVIALEARDN
                     GMLGVTLRYPYEVRKEADYFDDIPDEKITKDMLDLAKHIVETKKAHFEPARFEDRYEE
                     ALKDLLKKKQSGQKIEAPREREPSKVVNLMDALRRSVETDRGGERRKPARAAGNPRAA
                     KKAGRSSARAKKAG"
BASE COUNT          348 a          509 c          562 g          269 t
ORIGIN      
        1 cggcggaaag aagggcggcg tgctgacggt ggacttcacg gtcctcggca taccctgtct
       61 cggcctcaac ggcggcccgg tgttcaagca caacgaagcc ttctcgttcc agatcgcgac
      121 cgaagaccag gcagagacgg accgctactg gaacgcaatc gtcggcaatg gcgggcagga
      181 gagccagtgc gggtggtgca aagacaagtg gggaatttcc tggcagatca cgccgcgcgt
      241 gctgaccgac gcgctcgcgg ctggcggcga tgaagccaag cgcgctttcg ctgcgatgat
      301 ggaaatgggg aaaatcgacg tcgccgcgat cgaggcggcg cggcgcggct gaaggtctgg
      361 attcgtgccc gactggaatc agtcctcgac gcgctccacc tcgaactgga cgcgctcgcc
      421 gacatggcag cggtggccct gcccggcggc cagcagctcg cgcgcctcgt ccgccgtttc
      481 cgcctcgacg caccattcct cggcgcgcgt caccagcatc gtggcgtgaa acgtcctcgg
      541 cgcgggggcg ggcctttgtt tgagcgcggg agcagggcgt tgtttcagtg cggcgacggc
      601 catcacgcgc ttccttggcg aatcacttcg ggaatgcatt ctagccgcga gcgcaagcga
      661 gaacaaatcg acaacgcaac catagattcc gcggtgaatc gtccgctacc ctggattcgg
      721 aacgtgtgtg ctttatatcc gttgagtcgc cggtatttga tgtcgaggag ttgcccgtgg
      781 ccccgcgtgc gtactggaaa ggctatttga agctctccct ggtctcgtgc ccgatcgcgc
      841 tctttcccgc gaccagcgag cgcgaaaaga tcagcttcca ccagctcaac aagagcaccg
      901 gaaatcgcat caagtaccgc aaggtcgatg ccgacaccgg cgacgaggtc gaggcgcccg
      961 acatcatcaa gggttacgag gtgggcaagg gtgaatacct cgagctcgag ccggaagaac
     1021 tcgaagcgat cgcgatcgag agcaagcgca cgatcgagat cgacgaattc gtgccgcgca
     1081 aggagatcga cgagctctat ctcaacagcc cgtattacat cgtgcctgat ggcgaggtcg
     1141 gacagcaggc gttcgcggtg atccgcgaag ccatccgcca ggagggaatg gttgcgatcg
     1201 gcaaggtggt gttcacctcg cgcgagcacg tgatcgcgct cgaagcgcgc gacaacggca
     1261 tgctgggcgt gaccttgcgc tatccctacg aggtgcggaa ggaagcagac tatttcgacg
     1321 atattcccga cgaaaagatc accaaggata tgctcgacct cgccaagcac atcgtcgaga
     1381 ccaagaaggc gcatttcgag cccgcccggt tcgaggaccg gtacgaggag gcgctcaagg
     1441 acctgctgaa gaagaagcag tccgggcaga agatcgaggc gccgcgcgag cgggaaccgt
     1501 cgaaggttgt caatctcatg gatgcgctgc gccgcagcgt cgagaccgac cgcggcggcg
     1561 agcggcgcaa gccggcgcgc gcggccggca accctcgcgc ggccaagaag gccgggcgct
     1621 cgagcgcgcg ggcaaagaag gcgggctaga gcgggatgat tcctggtcga atcatcatcc
     1681 cgctccag
//
LOCUS       DASYJA010000141         2836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1385100, whole genome shotgun sequence.
ACCESSION   DASYJA010000141 DASYJA010000000
VERSION     DASYJA010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385100"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="VGO02_02940"
     CDS             complement(<1..959)
                     /locus_tag="VGO02_02940"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEV7476673.1"
                     /translation="MTDSPTPAETTAESAPAQRAARPWRIVLLALALVAGLATLYWVD
                     VRQRLSGAQHELASRLRAAENSAREASTVARAAQDSLLASQARIAALEARLAESQSQQ
                     LALEALYSDLTRNRDEWQLAEIEQVLAIASQQLQLAGNVRAALLALQLAEARLARTNR
                     PQFVPIKQALARDIERLRALPAIDMPAMSMRLDSLVAAVDALPLAFEERSERPAAAKD
                     AGADRGFWSRLGAEIWGELRQLVVVRQMNAPEPPLLPPTQAYFLRENLRLRLLNARMS
                     LLTRDEAGYREDLRAAQAWIRRYFDPRSKQTADALAQLKGLAGA"
     gene            complement(956..1618)
                     /locus_tag="VGO02_02945"
     CDS             complement(956..1618)
                     /locus_tag="VGO02_02945"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEV7476674.1"
                     /translation="MIEAAGGRAFLFPAVEIEPLPVTAIAPGRIDMAVFISPTAVREG
                     LRQLGALPPNLRIVALSRGTRRELERHGVAGALAAEAGADSEALLALPQLQQLAGQRV
                     AIFRGEGGRGLLGDTLRERGATVEYVECYRRVRPRSDPAPLAAAWRSGELHAVTVSSA
                     QGLGNLFAMLEGGLLRGLPLFVPHERVADEARRRGAREAIAAGPGDDEMLASLVAYFE
                     AR"
     gene            complement(1690..2592)
                     /gene="hemC"
                     /locus_tag="VGO02_02950"
     CDS             complement(1690..2592)
                     /gene="hemC"
                     /locus_tag="VGO02_02950"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEV7476675.1"
                     /translation="MKLTIATRRSRLALWQAEHVKGLLQSRGHDVGLLPLSTRGDELL
                     DQRLDKVGGKGLFVKELESALADGRAALAVHSMKDLPAGLPPGFAIAAILEREDPRDA
                     FVSSRYDSLGNLPEKAVVGTSSLRRAAQILERHPRLEAKLLRGNVETRLAKLDRGEYD
                     AVILAVAGLVRLGLASRIRSRLDPDESLPAPGQGALGIECLASRQDIADLLAPLADAA
                     AATCVRAERAVSRAFGGSCTIPLGAFAELDGKQLRLRALVAAADGRRVARTDVTGDAG
                     NPEALGRLAAEQLTAKGAREILAS"
     gene            complement(2589..>2836)
                     /locus_tag="VGO02_02955"
     CDS             complement(2589..>2836)
                     /locus_tag="VGO02_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEV7476676.1"
                     /translation="VRTAAGEHLIEEPLVALEREFAARLVRIHRNCLVARAAIRGFER
                     NPGDDESQWLVVLEGLAERLPVSRRQWPAVRELVAEK"
BASE COUNT          372 a         1042 c         1029 g          393 t
ORIGIN      
        1 gcgccggcca ggcccttcag ctgcgccagc gcgtccgccg tctgcttcga gcgcggatcg
       61 aagtagcggc ggatccaggc ctgggcggcg cgcaggtcct cgcggtagcc cgcttcgtcg
      121 cgcgtgagca gcgacatgcg cgcgttgagc aggcgcaggc gcaggttctc gcgcaggaag
      181 tacgcctgcg tcggcggcag caggggcggc tcgggcgcgt tcatctgccg caccaccacc
      241 agctggcgca gctcgcccca gatctcagcg cccaggcgcg accagaagcc acggtctgcg
      301 cccgcatcct tggcggccgc cggccgttcg ctcctttcct caaacgcgag cggcagcgcg
      361 tccaccgcgg ccaccaggct gtccagccgc atgctcatgg cgggcatgtc gatcgccggc
      421 agcgcccgca gccgctcgat gtcgcgcgcc agcgcctgct tgatcggcac gaactgcggc
      481 cggttggtgc gcgcgaggcg cgcctcggcg agctgcagcg cgagcagcgc ggcgcgcacg
      541 ttgccggcga gctgcagctg ctgcgaggcg atggcgagca cctgctcgat ctcggcgagc
      601 tgccattcgt cgcggttgcg cgtcagatcc gagtagagcg cctcgagcgc gagctgctgg
      661 ctttgcgact cggcgagccg cgcctcgagc gccgcgatgc gcgcctgcga ggcgagcagc
      721 gaatcctgcg cggcgcgcgc caccgtgctc gcctcgcgcg cgctgttctc ggcggcgcgc
      781 agccggctcg caagctcgtg ctgggcgccg ctcaggcgct ggcgcacgtc gacccagtac
      841 agcgtggcga gccccgcaac cagcgcgagc gccagcagga cgatgcgcca gggacgcgcg
      901 gcgcgctgcg ccggcgcgct ttcggcagtc gtttcggcag gcgtggggct gtcggtcatc
      961 gcgcctcaaa gtatgccacc agggacgcga gcatttcatc gtcccccggc ccggcggcga
     1021 tcgcctcgcg cgcgccgcgg cggcgcgcct cgtcggcgac gcgctcatgg ggcacgaaca
     1081 gcggcaggcc gcgcagcagc ccgccctcca gcatggcgaa aaggttgccc aggccctgcg
     1141 ccgaggacac cgtgacggcg tgcagttccc ccgagcgcca cgccgcggcg agcggcgccg
     1201 ggtcgctgcg cgggcgcacg cggcggtagc actcgacgta ttcgaccgtc gcaccccgct
     1261 cgcgcagggt gtcgccgagc aggccgcgtc cgccttcgcc gcggaagatc gcaacgcgct
     1321 ggccggcgag ctgctgcagc tgcggcagcg cgagcagcgc ctcgctgtcg gcgcccgcct
     1381 cggcggcgag cgcgcccgcg acgccgtgcc gctcgagctc gcggcgcgtg ccgcggctga
     1441 gcgcgacgat cctcaggttc ggcggcaggg caccgagctg cctgagcccc tcgcgcaccg
     1501 ccgtcgggct gatgaatacc gccatgtcga tgcggccggg cgcgatcgcg gtcacgggca
     1561 gcggctcgat ctcgactgcg gggaacagga acgcccggcc acccgctgct tcgatcatct
     1621 ttgccaggcc ttgcgcgagt gatgcgggac gcgtgacgac gatcccgcgg ttttgcagag
     1681 aagcgttttt caagaagcga gaatctccct ggctcccttg gcggtgagct gctccgcagc
     1741 cagccgtccg agcgcctccg gattgcccgc gtcgcctgtg acgtctgtgc gcgccacccg
     1801 gcggccgtcg gccgcagcca ccagcgcgcg cagccgcagc tgcttgccgt ccagctccgc
     1861 gaacgccccc agggggatcg tgcagctgcc gccgaacgcc cggcttaccg cgcgctcggc
     1921 acgcacgcag gtagcggctg cggcgtcggc aagcggcgcg agcaggtccg cgatgtcctg
     1981 gcgcgaagcg aggcattcga tgccgagcgc cccctggccg ggcgccggca ggctctcgtc
     2041 gggatcgagg cgcgagcgga tgcggctcgc gagccccagc ctcaccagcc cggccacggc
     2101 gaggatcacc gcgtcgtact cgccgcggtc gagcttggcg agccgggtct ccacgttgcc
     2161 gcgcagcagc tttgcttcca gacgcggatg gcgctcgagt atctgggcgg cgcgccgcaa
     2221 gctggatgtg ccaacgaccg ctttttcagg caggtttccc aaagagtcgt acctgctgga
     2281 aacgaatgcg tcccgcggat cctcgcgttc gaggatcgcg gcgatggcga agcccggcgg
     2341 cagcccggca ggcaggtcct tcatcgaatg cactgcgagc gcggcgcgcc cgtcggcgag
     2401 cgcgctctcg agctccttca cgaacaggcc cttgccgccg accttgtcga ggcgctggtc
     2461 gagcagctcg tcgccgcgcg tcgacagcgg cagcaggccg acgtcgtggc cgcgactttg
     2521 gaggaggcct ttcacatgct ccgcctgcca caatgcgagg cggctgcggc gggttgcgat
     2581 ggtgagcttc atttctcggc tacgagctcg cgtaccgccg gccactggcg gcggctgacc
     2641 ggcaggcgct cggcgaggcc ctcgagcacg accagccact gcgactcgtc gtcgccggga
     2701 ttgcgctcga agccgcggat ggcggcgcgg gcgaccaggc agttgcggtg gatgcgtacc
     2761 aggcgggcgg cgaattcgcg ctcgagcgcc accagcggct cctcgatcag gtgctcgccc
     2821 gccgcggtgc gcaccg
//
LOCUS       DASYJA010000142         4812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2110727, whole genome shotgun sequence.
ACCESSION   DASYJA010000142 DASYJA010000000
VERSION     DASYJA010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2110727"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VGO02_02960"
     CDS             <1..184
                     /locus_tag="VGO02_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476677.1"
                     /translation="PLGRRSPGDRRSSVVNWWKRLKQRWARLTEDPRAAAIREAREKQ
                     LAEWAAGQHDADPIHK"
     gene            546..770
                     /locus_tag="VGO02_02965"
     CDS             546..770
                     /locus_tag="VGO02_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476678.1"
                     /translation="MGYQYKLGKNWRLGGALVGLKSPTYNHGSFFIAPLPILTYDFGP
                     VQMNAIYVPAYGDYNEFAVFGFYFSLPLSK"
     gene            complement(748..1143)
                     /locus_tag="VGO02_02970"
     CDS             complement(748..1143)
                     /locus_tag="VGO02_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV7476679.1"
                     /translation="MKPSSYPADFALSVHTDHAGYPSKVLLARADLHGTLELLSAAWE
                     TLLGYGHREFRGKTLCQLAGSDQPDAVAAILDRENMQPVDLTLHCRDGKPKRLRLHRR
                     FDGDERKMFIVAEETQEAESRAPILKAAS"
     gene            complement(1213..2406)
                     /gene="gspF"
                     /locus_tag="VGO02_02975"
     CDS             complement(1213..2406)
                     /gene="gspF"
                     /locus_tag="VGO02_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003271030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HEV7476680.1"
                     /translation="MEAFRYQALDTAGRSVSGVVQADTARDARTQLRERGLLPSDVEK
                     KNALHSWARGISAAQLSLLTRQLATLLDSGLTLEQALAALVEEAEAPPTRELLAGVKA
                     EVMAGASLATALGSYPRSFPEFYRALVHGGEDSGALPTVLAHLADYLDARQALRQKTS
                     LALLYPVLVGFVALAIVTGLLIYVVPQVVQVFQQSRQALPWLTRALIALSGFLQAAWP
                     YLLLVLVAITAAAYLALRRPAYRRRWHALLLGLPWLGPLARGLDTSRFASTLAILVAG
                     GVPLLGALASAARAMGNAVMREAVERAIGRVREGVSLSRALGETRAFPTLLVHLVASG
                     ELSGKLEQMLERAAHLETRALERRLAVFLTLLEPVMILVMGAVVLMIVLAILLPIIEI
                     NQLVH"
     gene            complement(2407..3828)
                     /gene="gspE"
                     /locus_tag="VGO02_02980"
     CDS             complement(2407..3828)
                     /gene="gspE"
                     /locus_tag="VGO02_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HEV7476681.1"
                     /translation="MIPYAFAKAHGVLVSSLKKELAEVAVRSDAQSGALAELRRHLGV
                     PLRARRVEAEEFDELIAAAYNGAGVGAAALSDNLSQDIDLSRLLQEIPKVADLLDSRD
                     DAPVIRLINALLTQALRDGASDIHFEAFESRSVVRLRIDGTLRDLIEPARALHGAIVS
                     RLKIMAQLDIAEKRLPQDGRIALRVAGKPVDVRVSTIPTGHGERVVLRLLDQQAGRLD
                     LAQLGMDPATLAHMDRLIKEPHGIVLVTGPTGSGKTTTLYAALSRLDTTALNIMTVED
                     PIEYDLDGISQTPINARIDMSFARALRTILRQDPDVVMIGEIRDLETAQIAVQASLTG
                     HLVFATLHTNDAVSAVTRLVDMGVEPFLLASSLIGVGAQRLVRRLCLECRKPFVQENI
                     SYYSAVGCAACNRSGYRGRTGIYELLTVDDDLRRLIHDRAAEQTLRAHVVERGMKSLR
                     DDAMRWAAAGVISLEEAVRVTRE"
     gene            complement(3825..>4812)
                     /locus_tag="VGO02_02985"
     CDS             complement(3825..>4812)
                     /locus_tag="VGO02_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HEV7476682.1"
                     /translation="AATNSILINAPDSIYNNLRAALDKLDVRRAQVYVEALIAEMTAD
                     KAAEFGVQWQSLTGASGASSSARAFGGTNFGTTGQNILGIAANPATAGRGLNVGVVKG
                     TVNIPGVGEILNLSLLIRALESDNNANILSTPTLLTLDNEEARIVIGQNVPFITGQYA
                     LSGAATTPTPFQTIERRDVGLTLRVRPQISEGGTVRLQIYQEVSRVEDTTNPAGVITN
                     KRAVESMVLVDDGQIVVIGGLIQDSLRDTVEKVPVLGDIPLLGQLFTYNSRQRVKTNL
                     MVFLRPTVLRDAPRAKSLTDERYDYIMGQEYKAKPAPHPVLPEMDSPALAPR"
BASE COUNT          789 a         1574 c         1655 g          794 t
ORIGIN      
        1 cccgcttggg cgaagatctc ctggtgatcg tcgatcttcc gtcgtgaact ggtggaaacg
       61 gctcaagcag cgatgggcca ggctgaccga agacccgcga gccgcagcga ttcgcgaggc
      121 cagagaaaag cagcttgccg aatgggcggc cgggcagcac gacgccgatc cgatccacaa
      181 gtagcgctat gtgcgccggc gcaccgacga gatgacaagt tgttgggttt gcgtgcaggg
      241 gacatactcg cgcgcccctg atcaggggga cgcgcatgag acattgcccc tactggctgc
      301 taccccttct tttctgcgcg tcggacgttg tgctggcgga cgacgcctga ctcaatctca
      361 acgttcacgt ctacggcttc tcgtatcaca ccgaccggca agggacaaag caaaatggcg
      421 tcaacaacga gctcaatcta gggctcggac tcaactacac ggtccacgag gacgaacgcg
      481 gcatcgggtt cgtcgaggcc gggttctacc aggattcggg aagcaatacg gcgaagcttg
      541 caggggtggg ctaccagtac aagctgggca agaactggag gctcggaggc gcattggtcg
      601 ggctcaaaag cccgacctac aaccacggca gcttcttcat cgccccgctg ccgatcctca
      661 cctacgactt cggtccggtg cagatgaacg cgatctacgt tcccgcctac ggggactaca
      721 acgagttcgc cgtcttcggc ttctatttca gcttgccgct ttcaaaatag gcgcgcggct
      781 ctccgcttcc tgagtttcct cggcgacgat gaacatcttg cgctcgtcgc catcgaaccg
      841 gcgatgcagg cgcaggcgtt tgggcttgcc gtcccggcag tgcaacgtca gatccaccgg
      901 ctgcatgttc tcgcggtcga gaatcgccgc gacggcgtcc ggctggtccg atccggcgag
      961 ctggcacagc gtcttgcccc tgaactcgcg gtgtccatag ccgagcagcg tttcccacgc
     1021 cgcgctcagc agctcgagcg tgccgtgcag gtcggctctc gccagcagga ccttcgaggg
     1081 atatccggca tgatcggtat gcacgctcag cgcgaaatct gcggggtagc tggagggttt
     1141 catgcgcaca agatatccgt tggataacgc gcgatctgtg cgaccgcgca ctgtcggtgt
     1201 ttcgagacag attcaatgca ccagctggtt gatttcgatg atcggcagga ggatggcgag
     1261 cacgatcatc agcaccaccg cgcccatcac caggatcatc accggctcga ggagcgtgag
     1321 gaacaccgcc aggcgccgct cgagcgcccg ggtctcgagg tgcgcggcgc gctccagcat
     1381 ctgctcaagc ttgccgctca gctcgccgct cgccacgagg tgaaccagaa gtgtcgggaa
     1441 ggcgcgcgtc tcgccaagcg cccgcgacag gctcacgcct tcgcgcacgc gcccgatcgc
     1501 gcgctcgacc gcctcgcgca tcaccgcgtt gcccatggcg cgcgccgcgg aggccagcgc
     1561 gccgagcagc ggcacgccgc cggcgaccag gatggcgagc gtgctggcga agcgcgaagt
     1621 gtcgaggccg cgcgcgagcg gcccgagcca gggcaggccc aggaggagcg cgtgccaccg
     1681 gcggcggtac gcagggcgac gcagcgccag atacgctgcg gcagttattg caacaagaac
     1741 caggagaagg tagggccagg cggcctgcag gaagccgctc agcgcgatca gcgcgcgggt
     1801 gagccagggc agggcctgcc gcgactgctg gaacacctgc acgacctgcg gcaccacgta
     1861 tatcagcagc ccggtgacga tggcgagcgc cacgaagccg accaggaccg ggtagagaag
     1921 cgcgaggctg gttttctgcc gcagcgcctg gcgcgcgtcc aggtagtcgg caagatgggc
     1981 gagcaccgtc ggcagggcgc ccgagtcttc gccgccgtgc accagggcgc ggtagaactc
     2041 tggaaagctg cgcggatagc tgccaagcgc cgttgcgagc gatgccccgg ccatcacctc
     2101 ggccttgacg ccggcgagca attcccgcgt cggcggcgcc tcggcctcct cgaccagcgc
     2161 ggcgagggcc tgctccaggg tcaggcccga gtcgagcagc gtcgccagct gccgcgtgag
     2221 caggctcagc tgggcggcgg agatgccgcg cgcccaggaa tgcagcgcat tctttttttc
     2281 cacgtccgag ggcagcagtc cgcgctcgcg aagctgcgtg cgcgcgtcgc gcgccgtatc
     2341 ggcctgcacc acccccgaga cggaccggcc ggccgtgtcg agcgcctggt agcggaaggc
     2401 ctccatttac tcgcgcgtga cgcgcacggc ttcctcgaga ctgatcacgc cggcggcggc
     2461 ccatcgcatg gcgtcgtcgc gcaacgattt catgccgcgc tcgacgacgt gcgcgcgcag
     2521 cgtttgctcg gcggcgcggt cgtggatcag gcgacgcagg tcgtcatcga cggtgagcag
     2581 ctcgtagatg ccggtgcgtc cgcggtagcc ggagcggttg cacgcggcgc agcccactgc
     2641 ggaatagtag gaaatgtttt cctgcacgaa gggcttgcgg cattccaggc aaaggcgccg
     2701 caccaggcgc tgcgcgccaa cgccgatcag gctggaggcg agcaggaagg gctcgacgcc
     2761 catatccacc agccgggtga ccgcgcttac cgcgtcgttg gtgtgcagag tggcgaacac
     2821 gagatgcccg gtaaggctcg cctgcacggc aatttgtgca gtctccaggt cgcgaatctc
     2881 gccgatcatg accacgtcgg ggtcctggcg caggatggtg cgcagcgcgc gcgcgaagct
     2941 catgtcgatg cgcgcgttga tcggcgtctg gctgatgccg tcgaggtcgt actcgatcgg
     3001 gtcctccacc gtcatgatgt tgagcgccgt tgtatcgagc cgcgacagcg ccgcatacag
     3061 cgtggtggtc ttgcccgagc cggtcgggcc ggtgaccagc acgatgccgt gcggctcctt
     3121 gatcagccgg tccatgtgcg cgagcgtggc cgggtccatg ccgagctgcg cgaggtcgag
     3181 gcgcccggcc tgctggtcga gcaggcgcag caccacgcgc tcgccgtgcc cggtcgggat
     3241 ggtggagacg cgcacgtcga ccggcttgcc ggcgacgcgc agcgcgatgc gcccgtcctg
     3301 cggcaggcgc ttctcggcga tgtcgagctg cgccatgatt ttgagacgcg agacgatcgc
     3361 gccgtgcagc gcgcgcgccg gctcgatcag gtcgcgcagc gtgccgtcga tgcgcaggcg
     3421 caccaccgag cggctctcga aggcctcgaa atggatgtcc gaggcgccgt cgcgcagggc
     3481 ctgggtgagc agcgcgttga tgaggcggat gaccggcgcg tcgtcgcggc tgtcgagcag
     3541 gtcggcgacc ttcgggatct cctgcagcag gcgcgacagg tcgatgtcct gcgacagatt
     3601 gtcggagagc gccgcagcgc ctacccctgc gccgttgtag gccgcggcga tcagctcgtc
     3661 gaattcttct gcttctacgc gacgcgcacg cagcggcacg ccaaggtggc ggcgcaattc
     3721 cgccagcgcg cccgattgcg cgtcgctgcg caccgccact tcggcaagtt cttttttaag
     3781 agaagaaacc aaaacgccat gcgccttggc gaatgcgtag ggaatcatcg cggcgccagc
     3841 gcgggcgaat ccatctcggg cagcaccggg tgcggtgcgg gcttcgcttt gtactcctgc
     3901 cccatgatgt agtcgtagcg ctcgtcggtg agcgatttcg cgcgcggcgc gtcacgcagc
     3961 acggtggggc gcaggaacac catcaggttg gtcttgaccc gctggcgcga gttgtacgtg
     4021 aagagctggc cgagcagcgg gatgtcgccc aggaccggca ccttctcgac ggtgtcgcgc
     4081 agcgaatcct ggatcaggcc gccgatcacc acgatctgcc cgtcgtccac cagcaccatc
     4141 gattcgaccg cgcgcttgtt ggtgatgacc ccggccggat tggtcgtgtc ctcgacgcgc
     4201 gacacctcct ggtagatctg cagccgcacc gtgccgccct ccgagatctg cggccgcacc
     4261 cgcagcgtca ggccgacgtc gcgccgctcg atggtctgga acggcgtcgg cgtggtcgcc
     4321 gcgccggaga gcgcgtactg cccggtgatg aagggcacgt tctggccgat gacgatcctc
     4381 gcttcctcgt tgtcgagtgt cagcagggtc ggcgtggaga ggatgttggc gttgttgtcg
     4441 ctctctagcg cccggatcag caggctgagg ttgaggatct cgccgacccc gggaatgttc
     4501 accgtgccct tgaccacgcc gacgttcagg ccgcggccgg ccgtggccgg gttggcagcg
     4561 atgcccagga tgttctggcc ggtggtgccg aagttggtgc cgccgaaggc gcgcgccgag
     4621 ctggaggcgc cgctcgcccc ggtcaggctc tgccactgca cgccgaactc ggcggccttg
     4681 tcggcggtca tctctgcgat cagcgcctcg acgtagacct gcgcccggcg cacgtcgagc
     4741 ttgtccagcg ccgcgcgcag gttgttgtag atcgaatcgg gcgcgttgat gaggatcgag
     4801 ttggtcgccg ca
//
LOCUS       DASYJA010000143         6045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2293452, whole genome shotgun sequence.
ACCESSION   DASYJA010000143 DASYJA010000000
VERSION     DASYJA010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6045
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2293452"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..419)
                     /locus_tag="VGO02_02990"
     CDS             complement(177..419)
                     /locus_tag="VGO02_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEV7476683.1"
                     /translation="MDFDKQPFDKELDTRGLNCPLPILRTKKALTDLQSGQVLKVVAT
                     DPGAVKDFQTFSKQTGHELLQHGEANREFTFFMRKK"
     gene            435..1403
                     /locus_tag="VGO02_02995"
     CDS             435..1403
                     /locus_tag="VGO02_02995"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEV7476684.1"
                     /translation="MDKPGSEAAANWLVTVTLLPTEDPASRMRVLRTLESLGAAVIRE
                     GVYLLPDTQANRTSLDALGQYIFKSAGVAHVLQVSAGSAAQQQSFQRMFDRTQRYEEL
                     IKNVESLRVGFGHSDPSAIASVLHKQRREFETIVALDFFPNETRSRAEKTLADTDAAV
                     RKLMFPRQTQTGVGPGERITRRIWATRKPPWADRLACAWLIRRFVDPEGTVTWLDKSQ
                     PPPASAIGFGFEGANFANSESRVTYEEMLVQLGLTKNPALTRIGSIVHFLEAGGTPVP
                     EAAGVQTLLQGAVRRSQNEDQLLGEAEKTFDLLYEAYFEAPKARGA"
     gene            complement(1400..2857)
                     /locus_tag="VGO02_03000"
     CDS             complement(1400..2857)
                     /locus_tag="VGO02_03000"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEV7476685.1"
                     /translation="MRAKALTTMTALALLAACLPPRALAQNTLPDLGGAGDTALSPQT
                     ERRIGESIMRDIRRDPSYIDDPEVAEYLGGVGARLTQETAGARHDFEFFALRDPAINA
                     FALPGGFVGVHSGLLVATDTESELASVLGHEIAHVTQRHIARMIGQQQQMQIPVLAAI
                     AAAILLGRTRPDLAAGATAAVQAGAVSSQLAFSRDYEREADRVGLQALAGAGFDPSAM
                     AAFFDKMQRVARISDDGTMPGYLRTHPLTGDRIADAQNRAASLPYKQHLDTPEFQLVR
                     AKLRADVGDARDAMTHFESALRDGRYASEAAARYGLANAELRARRAREADDEVARLRK
                     SGAAGPMIETLAARVKQALGDSEGAAALLAQARARYPYSRPILYAHVAALLDAGRNQD
                     AAGQLVEAVRNYPHDPRLLVLQSKTYAALGNRLLQHQAQAEVYVLQGSLPAAIEQLTL
                     ARAAGDGDFYQLSVIDARLKDLRSQHTEETRDNKR"
     gene            2892..3377
                     /gene="moaC"
                     /locus_tag="VGO02_03005"
     CDS             2892..3377
                     /gene="moaC"
                     /locus_tag="VGO02_03005"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEV7476686.1"
                     /translation="MKKLSHFDSGGAAHMVDVGEKTATHRVAVASGRIAMRPATLRQV
                     AAGRARKGDVLGIARIAAIQAAKRTPELIPLAHPIALTRVAVDFHLDRKKSAIGLTAR
                     VECRGQTGVEMEALTAVAVGLLTIYDMLKAVDRGMTLGEIRLEEKRGGKSGVFTAKIR
                     P"
     gene            3374..3811
                     /gene="soxY"
                     /locus_tag="VGO02_03010"
     CDS             3374..3811
                     /gene="soxY"
                     /locus_tag="VGO02_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HEV7476687.1"
                     /translation="MNILRRTLVGAALALLLKPFAAAAAWSKEAFGAKTAADALKSLG
                     VSSPAPSKDIAIEAPQIAENGAVVPIEIQSNILGTTALTVLIEKNPFPLASRFTFMDG
                     ALPFVKLNVKMGETSDVRVIAQAGARYYTASKEIKVTIGGCGG"
     gene            3821..4135
                     /gene="soxZ"
                     /locus_tag="VGO02_03015"
     CDS             3821..4135
                     /gene="soxZ"
                     /locus_tag="VGO02_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HEV7476688.1"
                     /translation="MAAGPMKMRATLGNGYTDVRVLMTHPMETGQRKDPDGKLIPMHF
                     IQNLTVKVNGKTVVEGETSQAVSRNPVFTFRIKGGARGDKVEVSWVDTEGETNRTEAA
                     VA"
     gene            4135..4923
                     /gene="soxA"
                     /locus_tag="VGO02_03020"
     CDS             4135..4923
                     /gene="soxA"
                     /locus_tag="VGO02_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HEV7476689.1"
                     /translation="MRFFVSLVVVAVLPAFAQDAKKEIQRYQQMIAEGSPVELFELEG
                     EALWKKPQGPNQVSLEKCDLGQGAGVLKGAYARLPRYFKDADRVMDLEARLLHCMTSL
                     QGRSREEATRRVFGNDDRPSEMESLSAYIAAQSRGVPMAAGSSHPKEKAAFELGRKLF
                     FHRAGPWDFSCASCHGEADKRIRMQDLPELSKPQFARPVVATWPAYRVSNSQLKTIQW
                     RMNDCYRQMRMPEPVFASDATVALITYLTVTGKGEPYRGPGTKR"
     gene            4920..5513
                     /gene="soxX"
                     /locus_tag="VGO02_03025"
     CDS             4920..5513
                     /gene="soxX"
                     /locus_tag="VGO02_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HEV7476690.1"
                     /translation="MKRLVFFLMIFMAGTALGGSGADEVEAVLKRDFKARGQATMDRV
                     VQDGVQRVCTESDDKPSAALAKQLEADQMKTIVFPEGGLLGDWKNGERIAQGGRGLTW
                     SDRPGTPSEGSCYNCHQVGAQESSYGTIGPSLLGFGKSRGSGPDMQRYVYGKIYNAKA
                     YNLCSQMPRLGHSGTLTAEQIKDLVALLLDPASPVNR"
     gene            5520..>6045
                     /locus_tag="VGO02_03030"
     CDS             5520..>6045
                     /locus_tag="VGO02_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HEV7476691.1"
                     /translation="MQRREFLRLLALAAAAGGGLRAADSDAQAADALYELKPFGNVSL
                     LHITDTHAQLLPTYFREPNGADFLKRYSLKSNTRSAYAHSRLDFEKLARRYGKTGGFA
                     HLAALLKRLRATRPEALLLDGGDSWQGSATALWTRGADMIGAQRLLGVHFMTGHWEFT
                     YGAARVQQAVERELL"
BASE COUNT         1002 a         2067 c         2065 g          911 t
ORIGIN      
        1 cacgcccatg cggtcgacca cggcaacggt ggcctggtag ccgctgtcgc ggcatttctt
       61 cagcgccgcc tgcgcggctt tcaatgcggt ttcgggggtg agaattttct gggtgaaggt
      121 ggcctgggaa tgcgcttcac acagtaaaaa cattccaaac aggcaccata ccgccctcac
      181 ttcttcctca tgaagaaggt gaattctcgg ttcgcttcgc cgtgctgcag caattcatgg
      241 ccggtctgct tggagaaggt ctgaaagtcc ttgaccgccc ccggatcggt cgcgaccacc
      301 ttcagcacct gtccggactg aagatcggtg agggctttct tggtgcgcag gatcggtagc
      361 gggcagttca gcccgcgcgt atcgagttcc ttgtcgaagg gctgtttgtc gaagtccata
      421 aagtattatg ccgcatggac aaaccgggca gcgaggcggc cgcgaactgg ctggtgacgg
      481 tgacgctgct gcccaccgaa gaccccgcct cgcgcatgcg cgtgctgcgt acgctggagt
      541 ccctgggcgc cgccgtgata cgcgagggcg tctacctcct gccggacacc caggccaacc
      601 gcacctcgct cgatgcgctc ggccagtaca tcttcaagag cgccggcgtc gcgcacgtgc
      661 tgcaggtctc ggcggggtcg gccgcgcagc agcagtcgtt ccagcgcatg ttcgaccgca
      721 cccagcgcta cgaggagctg atcaagaacg tcgagtcgct gcgcgtcggc ttcggtcact
      781 ccgacccgag cgccatcgcc agcgtgctgc acaagcagcg gcgcgagttc gagaccatcg
      841 tcgcgctcga cttctttccg aacgagacgc gcagccgcgc cgagaaaacg ctcgccgaca
      901 ccgacgccgc ggtgcgcaag ctgatgttcc cgcgccagac gcagaccggc gtcggcccgg
      961 gcgagcgcat cacgcggcgc atctgggcga ccaggaagcc gccgtgggcg gaccgcctcg
     1021 cctgcgcctg gctgatccgc cgcttcgtcg atcccgaggg cacggtgacc tggctcgaca
     1081 agtcgcagcc gccgccggcg agcgcgatcg gcttcggctt cgagggcgcg aacttcgcca
     1141 acagcgagag ccgcgtcacc tacgaggaga tgctggtgca gctcggcctg acgaagaacc
     1201 ccgcgctcac gcgcatcggc agcatcgtgc acttcctgga agcgggcgga acgccggtcc
     1261 ccgaggccgc cggcgtgcag accctgctgc agggcgcggt gcgccgctcg cagaacgagg
     1321 accagctcct cggtgaggcc gagaaaacct tcgacctcct gtacgaggct tatttcgagg
     1381 ctccgaaggc acgcggcgcc tagcgcttgt tgtcgcgcgt ttcctcggtg tgctgcgagc
     1441 gcaggtcctt gaggcgcgcg tcgatcaccg acagttggta gaagtcgccg tcgcccgcgg
     1501 cgcgcgcgag ggtcagctgc tcgatggccg cgggcagcga gccctgcagc acgtagacct
     1561 cggcctgcgc ctggtgctgc agcagccggt tgccgagcgc ggcatacgtc ttcgactgca
     1621 ggaccaggag ccgcgggtcg tgcggatagt tgcgcaccgc ttcgaccagc tgccccgccg
     1681 cgtcctggtt gcgcccggcg tcgagcaggg ccgcgacgtg cgcgtagagg atcgggcggg
     1741 aatacggata gcgcgcgcgc gcctgcgcga gcagcgccgc ggcgccctcg ctgtcgccca
     1801 gcgcctgctt cacgcgcgcg gccagggtct cgatcatcgg cccggcggca ccgctcttgc
     1861 gcagccgcgc cacctcgtcg tcggcctcgc gcgcgcgccg cgcgcgcagc tctgcattgg
     1921 ccaggccata gcgcgccgcc gcctcgctcg cgtagcgccc gtcgcgcagc gcgctttcga
     1981 agtgcgtcat ggcgtcgcgc gcgtcgccca catcggcacg cagcttggcg cgcaccagct
     2041 ggaactccgg ggtgtccagg tgttgcttgt agggcaggct cgccgcgcgg ttctgcgcat
     2101 cggcgatgcg atcgccggtc aacgggtggg tgcgcagata gcccggcatc gtgccgtcgt
     2161 cgctgatgcg cgcgacgcgc tgcatcttgt cgaagaacgc agccatcgcg ctcgggtcga
     2221 agccggcgcc ggcgagggcc tgcaggccga cgcggtccgc ctcgcgctcg tagtcgcgcg
     2281 agaaggcgag ctggctcgac accgcgccgg cctgtaccgc ggcggtggcg ccggccgcga
     2341 ggtcgggccg cgtgcggcca agcaggatcg cggcggcgat cgccgccagg accgggatct
     2401 gcatctgctg ctgctgcccg atcatgcgcg cgatgtgccg ctgcgtgacg tgcgcgatct
     2461 cgtggccgag caccgaggcg agctccgact cggtgtcggt ggcgaccagc aggccgctgt
     2521 gcacgccgac gaagccgccg ggcaacgcga aggcgttgat cgccgggtcg cgcaaggcga
     2581 agaactcgaa gtcgtgccgc gccccggcgg tctcctgggt gagccgcgcg ccgacgccgc
     2641 ccaggtactc ggcgacctcc gggtcgtcga tgtagctcgg gtcgcgccgg atgtcgcgca
     2701 tgatgctctc gccgatgcgc cgctccgtct gcggcgagag cgcggtgtcg cccgcgccgc
     2761 ccaggtcggg gagcgtgttc tgcgccagcg cccgcggcgg caggcaagcc gccagcagag
     2821 caagcgcagt catcgtcgtg agtgccttcg cgcgcatgtg tttatgatag cgcccggaca
     2881 atacaggttc catgaagaaa ctcagccact tcgatagcgg cggcgccgcg cacatggtag
     2941 acgtcggcga aaagactgcc acccaccgcg ttgcggtcgc ctcggggcgc atcgcgatgc
     3001 gcccggcgac gctgcgccag gtcgccgccg gccgcgccag gaaaggcgac gtgctgggca
     3061 tcgcgcgcat cgccgcgatc caggctgcga agcgcacgcc cgagctgatc ccgctggccc
     3121 atccgatcgc cctgacgcgc gttgcagtgg attttcatct cgaccggaaa aaatccgcca
     3181 tcgggttgac cgcccgggtg gaatgccgcg gccagaccgg ggtcgagatg gaagccctca
     3241 ccgccgtggc ggtcggcctg ctcaccatct acgacatgct gaaggcggtc gaccgcggca
     3301 tgacgctcgg cgagatccgc ctggaggaga aacgcggcgg gaaatcaggg gtattcaccg
     3361 ctaaaatacg gccatgaaca ttcttcgcag aacactcgtc ggcgcagcgc tcgccctgct
     3421 gctgaagcct ttcgccgcgg ctgcggcgtg gagcaaggag gcgttcggcg ccaagaccgc
     3481 ggccgacgcg ctgaagagcc tcggcgtgtc gagcccggcg ccgagcaagg acatcgcgat
     3541 cgaggcgccg cagatcgccg agaacggcgc cgtggtgccg atcgagatcc agagcaacat
     3601 cctgggtacc accgcgctca cggtgctgat cgagaaaaac ccgttccccc tcgccagccg
     3661 cttcaccttc atggacggcg ccctgccctt cgtcaagctc aacgtgaaga tgggcgagac
     3721 ctccgacgtg cgcgtgatcg cgcaggccgg cgctcgctat tacacggcca gcaaggaaat
     3781 caaggtcacc atcggtggct gcggaggata aggagacgct atggctgcag gaccgatgaa
     3841 gatgcgcgcc accctgggta acggctatac cgacgtgcgc gtgctgatga cccacccgat
     3901 ggagacaggc cagcgcaagg accccgacgg caagctgata ccgatgcact tcatccagaa
     3961 cctcaccgtc aaggtgaacg gcaagaccgt ggtcgaggga gagaccagcc aggcggtgtc
     4021 gcgcaacccg gtgttcacct tccgcatcaa gggcggcgcc aggggcgaca aggtcgaggt
     4081 gagctgggtc gacaccgagg gcgagacgaa caggacggaa gccgcggtcg cctgatgcgg
     4141 tttttcgttt ctctggttgt cgttgccgtt cttcctgctt tcgcgcagga cgcaaaaaaa
     4201 gagattcagc gttaccagca gatgatcgcc gagggcagcc cggtggagct gttcgagctc
     4261 gagggcgagg cgctgtggaa gaagccgcag gggcccaacc aggtttcgct ggagaagtgc
     4321 gacctcgggc agggcgcggg cgtgctcaag ggcgcctacg cgcggctgcc gcgctacttc
     4381 aaggacgccg accgcgtcat ggacctggaa gcacgcctgc tccactgcat gacgagcttg
     4441 caggggcgca gccgcgaaga ggcgacgcgc cgcgtcttcg gcaacgacga ccgcccctcc
     4501 gagatggagt cgctctcggc ctacatcgcc gcccagtcgc gcggcgtgcc gatggcggcc
     4561 ggcagctcgc atccgaagga gaaggcagcg ttcgagctcg gccgcaagct gttcttccac
     4621 cgcgccgggc cctgggactt ctcctgcgcg agctgtcatg gcgaggccga caagcgcatc
     4681 cgcatgcagg acctgccgga gctgtccaag ccgcagttcg cacggccggt ggtcgccacc
     4741 tggcccgcct accgcgtctc caacagccag ctcaagacca tccagtggcg catgaacgac
     4801 tgctaccggc agatgcgcat gcccgagccg gtgttcgcgt cggacgcgac cgtcgccctc
     4861 atcacctacc tgacggtcac cggcaagggc gagccctacc gcggcccggg gacgaagcga
     4921 tgaagcgcct ggtttttttc ctgatgatat tcatggccgg gacggcgctc ggcggatccg
     4981 gcgccgatga agtcgaggcc gttctcaagc gcgacttcaa ggcgcgcggc caggccacca
     5041 tggacagagt cgtgcaggac ggcgtgcagc gcgtctgcac tgagagcgac gacaagccct
     5101 cggctgcgct ggcgaagcag ctggaagccg accagatgaa gaccatcgtc ttccccgagg
     5161 gcggcctgct cggcgactgg aagaacggcg agcgcatcgc gcagggcggc cgcggcctca
     5221 cctggagcga ccggcccggc acgccgagcg agggcagctg ctacaactgc caccaggtcg
     5281 gcgcgcagga gagctcctac ggcaccatcg ggccgagcct gctcggcttc ggcaagagcc
     5341 gcggcagcgg gcccgacatg cagcgctacg tctacggcaa gatctacaac gccaaggcgt
     5401 acaacctgtg ctcgcagatg ccgcgcctcg ggcactccgg caccctcacc gccgagcaga
     5461 tcaaggacct ggttgcgctg ctgctcgatc cggcttcgcc ggtgaaccgc taagcgcggt
     5521 tgcagcggcg cgaattcctc cgcctcctcg cccttgccgc cgccgcgggc ggcgggctgc
     5581 gcgcggcgga ctccgacgcc caggcggccg acgcgctcta cgagctgaag ccgttcggca
     5641 acgtctcgct gctgcacatc accgacacgc acgcccagct cctgccgact tatttcaggg
     5701 agccgaacgg cgccgatttc ctcaaaagat attcgctgaa gagcaatacg agatcggcat
     5761 acgcccacag ccgcctcgat tttgaaaagc tggcaagacg ctatggcaag accggcggct
     5821 tcgcccacct tgccgcgctg ctgaagcgcc tgcgcgcgac gcgccccgag gcgctgctgc
     5881 tggacggcgg cgacagctgg cagggctcgg ccaccgccct gtggacgcgc ggcgcggaca
     5941 tgatcggcgc gcagcgcctg ctcggcgtgc atttcatgac cgggcactgg gagttcacct
     6001 acggcgcggc gcgcgtgcag caggcggtgg agcgcgaatt gctgc
//
LOCUS       DASYJA010000144         4493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1748062, whole genome shotgun sequence.
ACCESSION   DASYJA010000144 DASYJA010000000
VERSION     DASYJA010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748062"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..178
                     /locus_tag="VGO02_03035"
     CDS             <1..178
                     /locus_tag="VGO02_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEV7476692.1"
                     /translation="PEGIDYVIPGNDDSSRAIRLYARGMADAVLEGRSQALEDTVAAS
                     RDEFVEVEEETGDK"
     gene            252..1079
                     /gene="tsf"
                     /locus_tag="VGO02_03040"
     CDS             252..1079
                     /gene="tsf"
                     /locus_tag="VGO02_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEV7476693.1"
                     /translation="MAEITAALVKELRELTGLPMMDCKKALIETNGDIKKAEELLRIK
                     SGAKASKAASRIAAEGVIGAYVSPDGKVGALVELNCETDFVAKNEEFVDFARSLAQTA
                     ATQNPRDLAQLASGKVEERRQALIQKIGENITIRHFKRLQAKGKLANYLHGVKIGVLV
                     DFEGPDDTAKEVAMHIAFAKPQYMTKDQVPADVVQREREIQLARAKESGKPPEIAAKM
                     VEGSLNKFLGELTLLGQPFVKDDKQTVEKILTAKKAKVNAYAFLVVGEGIEKAQPTH"
     gene            1084..1797
                     /gene="pyrH"
                     /locus_tag="VGO02_03045"
     CDS             1084..1797
                     /gene="pyrH"
                     /locus_tag="VGO02_03045"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV7476694.1"
                     /translation="MTPKYKRILLKLSGEALMGDDPYGINRQTIEAIVEEVAQVSRLG
                     VEMAVVIGGGNIFRGVAPGAAGMDRATADYMGMLATVMNALALSDAMRQAGIASRVQS
                     ALNIEQVVEPYIRGKAIRYLEEGKVVIFAAGTGNPFFTTDTAAALRGSEISAEIVLKG
                     TKVDGIYTADPKRDPRAQRYSKISFDEAIARNLKVMDATALTLCRDQKLPINVFSIFK
                     KDALRRVVLGEDEGTLVHV"
     gene            1826..2386
                     /gene="frr"
                     /locus_tag="VGO02_03050"
     CDS             1826..2386
                     /gene="frr"
                     /locus_tag="VGO02_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV7476695.1"
                     /translation="MVIADLKKQTEQKMDKSIQAFKADLGKVRTGRAHTGILDHVMVD
                     YYGAPTPLAQVAKVILLDSRTIGVTPFEKKMVQAIDKAIRDSDLGLNPATQGDTVRVP
                     MPSLTEERRKELIKVVKHEAENARVAVRNQRRDAIQHLKDGLKKSEVSENDERRAQDE
                     VQKMTDKHVAEIDRLLQEKEKELLAV"
     gene            2383..3165
                     /gene="uppS"
                     /locus_tag="VGO02_03055"
     CDS             2383..3165
                     /gene="uppS"
                     /locus_tag="VGO02_03055"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEV7476696.1"
                     /translation="MSKLPPPHESSTRVVPEHGAVPRHVAIIMDGNGRWAKSRRLPRI
                     AGHRRGVESVRASVTACAERGVEYLTLFAFSSENWRRPPEEVALLMQLFQMALSSEVE
                     KLHRNGVRLKVVGDLSRFDPNIRRLIDQGERLTADNKRLTLTIAANYGGRWDIMQALT
                     RLLKEKELPQGEVREELLAPYLAMSYAPEPDLFIRTGGEQRISNFLLWQLAYSELYFT
                     ERLWPDFDAAALDEAIASYRARERRFGRTSEQLEQSRRAEVA"
     gene            3186..3947
                     /locus_tag="VGO02_03060"
     CDS             3186..3947
                     /locus_tag="VGO02_03060"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEV7476697.1"
                     /translation="MATAAVLILGLLAALLFLPREWLAGVVAAVVALAAFEWARLCRL
                     SEHAARLYAAAAVTAYLILFYESADRIVFAAAAAYWLLVAVPWLLRGVAPRHAPALAV
                     AGFLILVPAGLAMVSLRPTQVLLVIGLVSIADSAAYFAGRAWGRHKLAPAISPGKSWE
                     GAIAGVVAGLAYAIICAAVSDEAAWLPYMGGAALLVVMSIFGDLFESAAKRQAAVKDS
                     GSLLPGHGGVLDRIDSATATLPLAAFLWPLLERIK"
     gene            3944..>4493
                     /locus_tag="VGO02_03065"
     CDS             3944..>4493
                     /locus_tag="VGO02_03065"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626680.1"
                     /note="catalyzes the formation of 2-C-methyl-D-erythritol
                     4-phosphate from 1-deoxy-D-xylulose-5-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEV7476698.1"
                     /translation="MNLTLLGATGSIGASTLDVVSRHAERYRVFALSAHGSVDALLEL
                     CRVHKPRYAVLSGTAESRDLRKKFAEQKTELLFGAAALEQVAAHPDCDAVMAAIVGAA
                     GLASTLAAARAGKRVLLANKEALVMTGPLMMAAARESGATILPVDSEHNAVFQCFSDK
                     SAVRRIVLTASGGPFRTTPLERL"
BASE COUNT          814 a         1471 c         1535 g          673 t
ORIGIN      
        1 gcccgagggg atcgactacg tgatcccggg caacgacgat tcgagccgcg cgatcaggct
       61 gtacgcgcgc ggcatggccg acgcggtgct cgaaggccgc agccaggcgc tggaagacac
      121 ggtcgccgcc tcgcgcgacg agttcgtcga ggtggaagag gaaacaggcg acaagtagag
      181 tgcgcgccgc ctgagccacg aaaaaggggc ctcgtgcccc tttttttcaa cctagtaagg
      241 agtttgcaag gatggccgag attactgcag cgctggtgaa ggagcttcgc gagctcaccg
      301 gcctgcccat gatggactgc aagaaggcgc tcatcgagac caacggcgat atcaagaagg
      361 ccgaagagct gctgcgcatc aagagcggtg ccaaggccag caaggcggcc agccgcatcg
      421 ccgccgaggg cgtgatcggc gcgtacgttt cgccggacgg caaggtcggt gctctggtcg
      481 agctcaattg cgagaccgac ttcgtcgcca agaacgagga attcgtcgat ttcgcgcgct
      541 ccctcgcgca gactgccgca acgcagaacc cgagggacct cgcccagctc gcgtcgggca
      601 aggtggaaga acgccgccag gcgctgattc agaaaatcgg cgagaacata accatccgcc
      661 acttcaagcg tctgcaggcc aaaggcaagc tcgccaacta tctgcacggc gtcaagatcg
      721 gcgtgctggt ggacttcgag gggccggacg acaccgccaa ggaagtggcg atgcacatcg
      781 ctttcgccaa gccgcagtac atgaccaagg accaggttcc cgccgacgta gtgcagcgcg
      841 agcgcgagat ccagctcgca cgggcgaagg aatcgggcaa gccgcccgag atcgccgcca
      901 agatggtcga gggctcgctg aacaagtttc tcggcgagct gacgctgctt ggtcagccct
      961 tcgtcaagga cgacaagcag accgtcgaga agattctcac tgcgaagaag gcgaaggtga
     1021 acgcctatgc gttcctggtt gtcggagagg gaatcgagaa ggcgcagccc acgcactaag
     1081 aacgtgacgc cgaaatacaa aaggatcctg ctcaagctct caggtgaagc cctgatgggg
     1141 gacgatccgt acggcatcaa ccggcagacc atcgaggcga tcgtcgagga agtggcgcag
     1201 gtgtcgcggc ttggcgtgga gatggccgtg gtgatcggcg gcggcaacat cttccgcggc
     1261 gtggcgccgg gcgcggccgg gatggaccgc gccaccgcgg actacatggg aatgctcgcc
     1321 acggtgatga acgcgctcgc gctgtcggat gccatgcgcc aggccggcat tgcgagccgc
     1381 gtgcagtcgg ctctcaacat cgagcaggtg gtcgagccgt atataagggg gaaagcgatt
     1441 cgctacctgg aggagggcaa ggtggtgatt ttcgccgccg gcaccggcaa cccgttcttc
     1501 accaccgata ccgccgccgc cctgcgcggc agcgagatca gcgccgagat cgtccttaag
     1561 ggcaccaagg tcgacggcat ctacaccgcc gatcccaagc gcgacccgcg cgcgcagcgc
     1621 tactcgaaga tcagcttcga cgaggccatc gcgcgcaacc tcaaggtgat ggacgccacg
     1681 gcgctgacgc tgtgccgcga tcagaagctg cccatcaacg tgttcagcat cttcaagaag
     1741 gacgcgctgc ggcgcgtggt gctgggcgag gacgagggca cgctggttca cgtatgattg
     1801 cccgaatcgg gaggccgcaa tggcattggt gatcgcggat ctgaagaagc agaccgagca
     1861 gaagatggac aagagcatcc aggcgttcaa ggccgacctc ggcaaggtcc gcaccggccg
     1921 cgcccacacc ggcattctcg accacgtcat ggtcgactat tacggcgcgc cgaccccgct
     1981 cgcccaggtc gccaaggtga tcctgctcga ctcgcgcacc atcggcgtca cgccgttcga
     2041 gaagaagatg gtgcaggcca tcgacaaggc gatccgcgac tccgacctcg gcctcaatcc
     2101 agcgacgcag ggcgacaccg tgcgcgtgcc gatgccttcc ctcaccgagg agcggcgcaa
     2161 ggagctgatc aaggtggtca agcacgaggc cgagaacgcg cgcgtcgcgg tgcgcaacca
     2221 gcggcgcgac gcgatccagc acctcaagga cggcctgaag aagagcgagg tgtcggagaa
     2281 cgacgagcgc cgcgcgcagg acgaggtgca gaagatgacc gacaagcacg tcgccgagat
     2341 cgaccggctg ctgcaggaga aggaaaagga gctgctggcc gtatgagcaa actaccccct
     2401 cctcacgaga gctcgacgcg cgtcgtgccc gagcacggcg cggtgccgcg ccacgtcgcc
     2461 atcatcatgg acggcaacgg gcgctgggcg aagagccgcc gcctgccgcg catcgccggc
     2521 caccgccgcg gcgtcgagtc ggtgcgcgcc agcgtcacgg cctgcgccga gcgcggcgtc
     2581 gagtacctca ccctcttcgc cttcagctcg gagaactggc ggcgcccgcc tgaggaagtg
     2641 gcgctgctga tgcagctttt ccagatggcg ctgtcgagcg aggtcgagaa gctgcaccgc
     2701 aacggcgtgc gcctgaaggt ggtcggcgac ctcagccgct tcgacccgaa catccgccgg
     2761 ctgatcgacc agggcgagcg cctgactgcc gacaacaagc gcctgaccct caccatcgcg
     2821 gccaactacg gcggccgatg ggacatcatg caggcgctga cgcggctgct gaaggagaag
     2881 gagctgccgc aaggggaagt tcgcgaggag ctgctcgcgc cctatctcgc catgagctac
     2941 gcgcccgagc ccgacctgtt catccgcacc ggcggcgagc agcgcatcag caacttcctt
     3001 ctctggcagc tggcgtactc ggagctctac ttcaccgagc gcctgtggcc ggacttcgac
     3061 gccgccgcgc tggacgaggc gattgcgtcg taccgtgcgc gcgaacggcg cttcgggcgc
     3121 accagcgagc aactcgagca gtcgcgccgc gccgaggtag cctgagcgca gggctcgccg
     3181 cgcggctggc gacggcggcc gtcctgatcc tgggactgct cgccgcgctg ctgttcctgc
     3241 cgcgcgagtg gctggccggc gtggtcgccg cggtcgttgc cctcgccgcg ttcgagtggg
     3301 cccggctttg ccgcctcagc gagcatgcgg cgcgcctcta cgccgcggcg gcggtcacgg
     3361 catacctcat cctgttctac gaatccgccg atcgcatcgt attcgcggcc gcagccgcct
     3421 actggctgct ggtggctgtg ccctggctgc tgcgcggtgt ggccccgcgt catgcgccgg
     3481 ccctcgccgt ggcggggttt ctgattctcg tgcccgccgg cctcgccatg gtctcgctgc
     3541 ggccgacgca ggtgctgctg gtgatcgggc tggtgtcgat cgccgacagc gcagcctatt
     3601 tcgcgggacg cgcctggggc cggcacaagc tggcaccggc gatcagcccg ggaaagagct
     3661 gggaaggcgc tatcgcagga gtggtagccg ggctggccta cgctatcatc tgtgcagcgg
     3721 tatctgacga agccgcctgg ctcccctaca tgggcggcgc ggcccttctg gtggtcatga
     3781 gcatcttcgg agaccttttc gaatcggcgg ccaagcggca ggcggcggtg aaggacagcg
     3841 gctcgctgct gcccgggcac ggcggggtac tggatcgcat cgacagcgcg accgccacgc
     3901 tgccgctggc ggcgttcctg tggcccttgc tggaaagaat caagtgaacc tcacgctcct
     3961 cggcgccacc ggctccatcg gcgccagcac gctcgacgtc gtttcgcggc atgcggagcg
     4021 ctaccgggtg ttcgcgctca gcgcccacgg gtcggtggac gcgctgctcg agctgtgccg
     4081 ggtgcacaag ccgcgctacg cggttctttc cggcacggct gagagccgcg atctgcggaa
     4141 aaagttcgcc gagcagaaga ccgagctcct gttcggcgcg gcggcgctcg agcaggtggc
     4201 cgcgcatccg gactgcgacg cggtgatggc cgccatcgtc ggtgccgcgg gcctcgcctc
     4261 gaccctcgcc gcggcgcgcg ccggcaagcg cgtcctgctc gcgaacaagg aggcgctcgt
     4321 gatgaccgga ccgctgatga tggccgcggc gcgcgagagc ggggcgacga tcctgcccgt
     4381 ggatagcgag cacaatgccg tttttcaatg cttctccgat aaatccgcgg tcaggagaat
     4441 tgtgctgacc gcctccggcg ggccgttccg caccacgccg ctcgagcgcc tcg
//
LOCUS       DASYJA010000145         2648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2383761, whole genome shotgun sequence.
ACCESSION   DASYJA010000145 DASYJA010000000
VERSION     DASYJA010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2383761"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VGO02_03070"
     CDS             <1..308
                     /locus_tag="VGO02_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990531 - phospholipid-translocating
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005548 - phospholipid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEV7476699.1"
                     /translation="SAGVVVGRVASIRFDGESYEAFVTMNLEAKYQFPRDTTAKILTS
                     GILGEQYVGLEAGGDTKMLEAGGTLRLTQSAVVLENLISQFLFNKAAEGNEKGNEKK"
     gene            305..1033
                     /locus_tag="VGO02_03075"
     CDS             305..1033
                     /locus_tag="VGO02_03075"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEV7476700.1"
                     /translation="MSRLVLFLAILAASGCATSPDADPRDPLEPMNRAVYKFNDTFDE
                     AIAKPVATVYRDLLHVEIRSRVSNFFSNIQDIFIGVNNLLQGKLQDGVEDWARFAFNS
                     TIGLAGIHDVASDMGIEKHNEDFGQTFGRWGAGPGPYLILPFLGSSTLRDATGTAVDY
                     YFAPLAEARPIALRNSLYAVYFVNTRAELLEASDILAQAALDRYVFQRDAYLQRRRSL
                     IYDGNPPREREPEEVNDKPDSSVK"
     gene            1036..1656
                     /locus_tag="VGO02_03080"
     CDS             1036..1656
                     /locus_tag="VGO02_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7476701.1"
                     /translation="MKNISRLLLVVSSAFLVKGTAAQEFMPPDVLVKNVTTEVVEIIA
                     KDKEIRSGNRAKLIEVIDAKVLPHFNFGSMTALAMGQNWRTATPEQKKQLIDAFRTLL
                     VRTYASALAAYSEQKFDYRPLRAKPTDTDVTVQVRVIQPGAQPVPIDYSMEKTPSGWK
                     VYDVMVGGVSLVANYRTEFANVVRNSGIDGLIKDLQTKNRVLEQKK"
     gene            1656..1937
                     /locus_tag="VGO02_03085"
     CDS             1656..1937
                     /locus_tag="VGO02_03085"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEV7476702.1"
                     /translation="MRREDGRIVLSGPVTLANVAQVLEEGRRHLAEGAATVDLAEVSE
                     LDSSALALLLAWLREAKAAGRALAFTNLPESLQTIARLYGVQDLLPAAR"
     gene            1934..>2648
                     /locus_tag="VGO02_03090"
     CDS             1934..>2648
                     /locus_tag="VGO02_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7476703.1"
                     /translation="MNAIEVRGIEKQYGKGSGRLRALDGVTLDIAQGEFFGLLGPNGA
                     GKTTLINVIAGLARADAGSAAVMGADVIADYRRARRMLGVVPQELVFDPFFSVRETLR
                     IQSGYYGIRGNDAWIDEVMHHLDLTAKADANMRSLSGGMKRRVLVAQALVHKPPVIVL
                     DEPTAGVDVELRQGLWHFVRRLNRDGHTIVLTTHYLEEAEALCGRIAMLKAGRVVALD
                     TTRNLLSRFAGLTVRLVAAE"
BASE COUNT          471 a          925 c          862 g          390 t
ORIGIN      
        1 agagcgccgg cgtggtcgtc ggccgcgtcg ccagcatccg cttcgacggc gagtcctacg
       61 aagctttcgt caccatgaac ctcgaggcga agtaccagtt cccgcgcgac acgaccgcca
      121 agatcctcac ctccgggatc ctcggcgagc agtacgttgg cctggaggcg ggcggcgaca
      181 cgaagatgct ggaggccggc ggcacgctgc gcctgacgca gtcggcggtg gtgctggaga
      241 acctgatcag ccagttcctc ttcaacaagg ccgccgaagg caatgagaaa gggaatgaga
      301 agaagtgagc cggctcgtcc ttttcctcgc catcctggca gcgtcgggct gcgccacctc
      361 gccggacgcg gacccgcgcg acccgctcga gccgatgaac cgggcggtgt acaagttcaa
      421 cgacactttc gacgaagcga tcgccaagcc ggtggcgacc gtctaccgcg acctgctgca
      481 cgtcgagatc cgcagccgcg tcagcaactt cttctccaac atccaggaca tcttcatcgg
      541 cgtcaacaac ctgctgcagg gcaagctgca ggacggcgtc gaggactggg cgcgcttcgc
      601 cttcaacagc accatcggcc tcgccggcat ccacgacgtc gcctccgaca tgggcatcga
      661 gaagcacaac gaggatttcg gccagacttt cggccgctgg ggcgccggcc cgggccccta
      721 tctcatcctg ccgttcctcg gctcgagcac gctgcgcgac gccaccggta ccgcggtgga
      781 ctactacttc gcgccgctcg ccgaggcccg gccgatcgcc ctgcgcaact cgctgtatgc
      841 ggtctacttc gtcaacacgc gcgccgagct gctcgaggcg agcgacatcc tggcgcaggc
      901 ggcgctcgac cgctacgtct tccagcgcga cgcgtacctg cagcgccggc gcagcctgat
      961 ctacgacggc aacccgccgc gcgaacgcga gcccgaagag gtcaacgaca agcccgactc
     1021 atccgttaag tgaacatgaa aaatatcagc cgcctgctgc tcgtcgtgtc gagtgctttt
     1081 ctggtaaaag gcactgcggc gcaggagttc atgccgcccg acgtgctggt gaagaacgtc
     1141 accaccgaag tcgtcgagat catcgccaag gacaaggaga tccgctccgg caaccgcgcc
     1201 aagctgatcg aggtgatcga cgccaaggtg ctgccgcact tcaacttcgg ctcgatgacc
     1261 gcgctcgcca tggggcagaa ctggaggacg gcgacccccg agcagaagaa gcagctgatc
     1321 gacgccttcc gtacgctgct cgtgcgcacc tacgcgagcg cgctcgccgc ctacagcgag
     1381 cagaagttcg actaccgccc gctgcgcgcc aagccgaccg acaccgacgt cacggtgcag
     1441 gtgcgcgtga tccagcccgg cgcgcagccg gtgccgatcg actacagcat ggagaagacg
     1501 ccgagcggct ggaaggtgta cgacgtcatg gtgggcggcg tgagcctggt cgccaactac
     1561 cgcaccgagt tcgccaacgt ggtgcgcaac tccggcatcg acggcctgat caaggacctg
     1621 cagacgaaga accgcgtgct ggagcagaag aagtgatgcg ccgcgaagac ggccgcatcg
     1681 tgctgtccgg gccggtgacg ctcgccaacg tcgcgcaggt cctcgaggaa ggcaggcgcc
     1741 acctcgccga aggcgccgcc acggtcgacc tcgccgaggt gagcgagctc gactcctcgg
     1801 cgctcgcgct gctgctcgcc tggctgcgcg aggcgaaggc cgcgggccgg gcgctcgcgt
     1861 tcaccaacct gcccgaatcg ctgcaaacca tcgcgcgcct gtacggcgtg caagacctgc
     1921 tgcccgctgc ccgctgaacg ccattgaggt ccgcggtatc gagaagcagt acgggaaggg
     1981 aagcgggcgg ctgcgcgcgc tcgatggcgt cacgctcgac atcgcgcaag gcgagttctt
     2041 cggcctgctc gggcccaacg gcgccggcaa gaccacgctc atcaacgtga tcgccggcct
     2101 cgcgcgcgcc gacgcgggca gcgccgcggt gatgggcgcc gacgtcatcg ccgactaccg
     2161 ccgcgcccgc cgcatgctgg gcgtcgtgcc gcaggaactg gtgttcgacc cgttcttctc
     2221 ggtgcgcgag acgctgcgca tccagtcggg ctactacggc atccgcggca acgacgcctg
     2281 gatcgacgag gtgatgcacc acctcgacct cactgccaag gccgacgcca acatgcgctc
     2341 cctttccggc ggcatgaagc gccgcgtgct ggtggcgcag gcgctggtgc acaagccgcc
     2401 ggtgatcgtg ctcgacgagc cgaccgcggg cgtcgacgtc gagctgcgcc aggggctgtg
     2461 gcatttcgtg cgccgcctga accgcgacgg ccacaccatc gtgctcacca cgcactacct
     2521 cgaggaagcc gaggcgctgt gcgggcgcat cgcgatgctc aaggcgggcc gggtggtggc
     2581 gctcgacacg acgcgcaatc ttctctcacg cttcgcgggc ctcaccgtgc gactggttgc
     2641 cgcggagg
//
LOCUS       DASYJA010000146         3768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1023151, whole genome shotgun sequence.
ACCESSION   DASYJA010000146 DASYJA010000000
VERSION     DASYJA010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023151"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1615
                     /locus_tag="VGO02_03095"
     CDS             <1..1615
                     /locus_tag="VGO02_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV7476704.1"
                     /translation="FLIGANPTSNHPVAATWMKNAAQRGAKLIYADPRRGDIARHAWR
                     YLQFKPDTDVAMLNAMIHTIIEEGLVDQKFIDDRTSGFDALKENAKDYSPEKMASICG
                     IPAETIREVARAFATSKASMILWGMGISQHVHGTDNARCLIALCLMTGQVGKPGSGLH
                     PLRGQNNVQGASDSGLIPMMYPDYQRVATPEAQQRFEKLWGQPLDPKPGLTVVEIVNA
                     AYDGKIRGMYIMGENPAMSDPDVDHARAGMAKLEHMVVQDIFLTETCYLADVVLPATA
                     WPEKDGTVTNTDRMVQLGRKAVDAPGEARPDLWIIVELARRLGLDWSYQHPKQVFDEM
                     RQCMDSIKGITWERLERDSTVTYPCENEGDPGEPVVFVTHFPTPTGRAKFVPADLIRA
                     DERPDAEFPLVLITGRQLEHWHTGAMTRRAGVLDAIEPEPTASLHPSDLKNLKVKPGE
                     LVTVESRRGKISLYARADAGTPRGSVFVAFAFYEAAANLLTNPALDPFGKIPEFKYCA
                     VRVAKGGTRPADPGYGRGRSLEEIGKNQF"
     gene            complement(1612..2760)
                     /locus_tag="VGO02_03100"
     CDS             complement(1612..2760)
                     /locus_tag="VGO02_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7476705.1"
                     /translation="MTRVRLAALAAAALAIAAGHEPARAQVPGVSATTIVIGQSAPLS
                     GANAELGNDIRNGALAYFKKVNEAGGVHGRKIELASLDDANAVPRAEENTKKLVEEQG
                     VFALFGYASATLSRPALPFVDKHRVPFLSPFTGADPMRVFNKYVYNMRGSYAEELEKI
                     VDHFAPLGVKRFSIVYYDDVVGRENFTAVERALKKRNLAVVSVAAWKDRAKPDIDGGV
                     QSIARGEPDVVILTTLYKATADFVRAAHKAGLGFQMASNSFPGASPLAKELGKQGAGF
                     IVATVVPPPSKRSLAIVQEYQVAIEKQLGKKDLSFTSLESYIAAKATVEALRRAGPKL
                     TRESFMQALDGMKSFDAGGYVMSFSPSNHNGSSFVELTVLGRDLKFNY"
     gene            2920..>3768
                     /locus_tag="VGO02_03105"
     CDS             2920..>3768
                     /locus_tag="VGO02_03105"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEV7476706.1"
                     /translation="MPSDIEIAQAARMQRIAAVARERLGIGEEHLEPYGHYKAKVSLK
                     YLETLKGKKDGKLVLVTAISPTPAGEGKTTTTVGLGDALNHIGKKAIICLREPSLGPV
                     FGVKGGAAGGGYAQIVPMEDINLHFTGDFGAIQLANNLLAALIDNHISHGNELGIDIR
                     RIQWKRVLDMNDRALRDITVGLGGTANGYPRQDGFDIVVASEVMAIFCLSTSLDDLKQ
                     RLGNIVFGYTRDQKPVRAKDLQAHGAMTVLLKDALAPNLVQTLENNPAFVHGGPFANI
                     AHGCNSV"
BASE COUNT          690 a         1267 c         1227 g          584 t
ORIGIN      
        1 cttcctcatc ggcgccaacc cgacctcgaa ccacccggtg gcggccacct ggatgaagaa
       61 cgccgcccag cgcggcgcca agctgatcta cgccgacccg cggcgcggcg acatcgcgcg
      121 ccacgcctgg cgctacctgc aattcaagcc cgacaccgac gtcgccatgc tgaacgcgat
      181 gatccacacg atcatcgagg aaggtctggt tgatcagaag ttcatcgacg atcggacaag
      241 tggatttgat gcattgaaag aaaatgccaa ggactactcg ccggagaaga tggcgtcgat
      301 ctgcggcatc ccggccgaga ctatccgcga ggtagcgcgc gccttcgcca cctccaaggc
      361 gtcgatgatc ctgtggggca tgggaatctc gcagcacgtc cacggcaccg acaacgcgcg
      421 ctgcctgatc gcgctgtgcc tgatgaccgg ccaggtcggc aagcccggca gcggcctgca
      481 tccgctgcgc ggccagaaca acgtgcaggg cgcttccgac tcggggctga tcccgatgat
      541 gtatcccgac taccagcgcg tggcgacgcc ggaagcgcag cagcgcttcg agaagctctg
      601 gggccagccg ctcgacccga agcccggcct caccgtggtc gagatcgtca acgccgccta
      661 cgacggcaag atccgcggca tgtacatcat gggcgagaac ccggcgatgt ccgacccgga
      721 cgtcgaccac gcgcgcgccg gcatggcgaa gctcgagcac atggtggtgc aggacatctt
      781 cctcaccgag acctgctacc tcgccgacgt ggtgctgccg gccaccgcct ggccggagaa
      841 ggacggcacg gtcaccaaca ccgaccgcat ggtgcagctc ggccgcaagg cggtcgacgc
      901 gcccggcgag gcgcggcccg acctgtggat catcgtcgag ctggccaggc gcctggggct
      961 agattggtct taccaacacc ccaaacaggt ttttgatgaa atgcggcaat gcatggactc
     1021 catcaagggg atcacctggg agcgcctcga gcgcgactcg acggtgacct acccgtgcga
     1081 gaacgagggc gatccgggcg agccggtggt gttcgtcacg cacttcccga cgcccaccgg
     1141 gcgcgccaag ttcgtgccgg ccgacctgat ccgcgccgac gagcggcccg acgccgagtt
     1201 cccgctggtg ctgatcaccg gccgccagct cgagcactgg cacaccggcg ccatgacgcg
     1261 ccgcgccggc gtcctcgacg cgatcgagcc cgagccgacg gcgtcgcttc acccctccga
     1321 tctgaagaat ctgaaggtga agccaggcga gctggtgacg gtggaatccc ggcgcggcaa
     1381 gatttcgctg tatgcaaggg cggacgccgg cacgccgcgc ggctcggtct tcgtcgcgtt
     1441 cgccttctac gaggcggcgg cgaacctcct caccaacccg gcgctcgacc cgttcggcaa
     1501 gatcccggag ttcaagtact gcgcggtgcg ggtggcgaag ggcggcacgc gccctgctga
     1561 tccaggttac ggcaggggcc gcagcctgga agaaatcggc aagaaccagt tctagtagtt
     1621 gaatttcaaa tcgcgcccga gcaccgtcag ctcgacgaac gagctgccgt tgtggttgct
     1681 gggcgaaaag ctcatgacgt agccgccggc gtcgaagctc ttcatgccgt cgagcgcctg
     1741 catgaagctc tcgcgcgtca gcttcgggcc ggcgcggcgc agcgcctcga ccgtcgcctt
     1801 ggcggcgatg taggactcga gggaagtgaa ggagaggtct ttcttgccca gctgcttctc
     1861 gatcgcgacc tggtactcct gcacgatcgc cagcgagcgc ttggacggcg gcggcaccac
     1921 ggtggcgacg atgaagccgg cgccctgctt gccgagctcc ttggcgagcg ggctcgcgcc
     1981 gggaaaggag ttgctcgcca tctggaagcc gagccccgcc ttgtgcgccg cccggacgaa
     2041 gtcggcggtc gccttgtaga gggtggtgag gatcaccacg tcgggctcgc cccgggcgat
     2101 ggactgcacg ccgccgtcga tgtcgggctt ggcgcggtcc ttccacgccg ccaccgacac
     2161 caccgcgagg ttgcgcttct tcagcgcgcg ctccaccgcg gtgaagttct cgcggccgac
     2221 gacgtcgtcg taatagacga tcgagaagcg cttcacgccg agcggcgcga aatggtcgac
     2281 gatcttctcc agctcctcgg cgtagctgcc gcgcatgttg tagacgtact tgttgaacac
     2341 gcgcatcggg tcggcgccgg tgaacggcga gaggaacggc acgcggtgct tgtcgacgaa
     2401 cggcagggcg gggcgcgaca gcgtggcgct ggcgtagccg aagagcgcga acacgccctg
     2461 ctcctcgacc agcttcttgg tgttctcctc ggcgcgcggc accgcgttgg cgtcgtccag
     2521 ggaggcgagc tcgatcttgc ggccgtgcac gccgccggcc tcgttcacct tcttgaaata
     2581 ggcgagcgcg ccgttgcgga tgtcattgcc gagctcggcg ttggcgcccg agaggggtgc
     2641 cgactgaccg atgacgatgg tggtggcgct cacccccggc acctgcgcgc gcgccggctc
     2701 gtggccggct gcgatggcaa gcgccgcggc agccagcgcc gccaagcgaa ccctggtcat
     2761 acgagcctct tcccttgtcc ggattgtctt ggcgcgcttg atggccgtca agagaaacgc
     2821 ttgatattag tcaagcaggg gcggcaagaa cctttacaaa acaaaatttt gccaaccaga
     2881 atccgccctc gggccggaac cacccctcag gaggaacaaa tgccgtcgga tatcgaaatc
     2941 gcccaggccg ccaggatgca gcgcatcgcc gccgtggcgc gcgaaaggct gggcatcggc
     3001 gaggagcacc tcgagcctta tggccactac aaggccaagg tctcgctcaa gtacctcgag
     3061 accctgaagg gcaagaagga cggcaagctg gtcctggtga ccgcgatcag cccgaccccg
     3121 gcgggcgagg gaaagaccac caccaccgtc gggctgggcg acgcgctgaa ccacatcggc
     3181 aagaaggcca tcatctgcct gcgcgagccc tcgctcggcc cggtgttcgg cgtgaagggc
     3241 ggcgcggcgg gcggcggcta cgcgcagatc gtgccgatgg aggacatcaa cctgcacttc
     3301 accggcgatt tcggcgccat ccagctcgcc aacaacctgc tcgccgcgct gatcgacaac
     3361 cacatcagcc acggcaacga gctgggcatc gacatccgcc gcatccagtg gaagcgcgtg
     3421 ctcgacatga acgaccgcgc gctgcgcgac atcaccgtcg gcctgggcgg caccgccaac
     3481 ggctatccgc gccaggacgg cttcgacatc gtcgtcgcct ccgaggtgat ggcgatcttc
     3541 tgcctgtcca cgtctttgga tgatctaaaa caaagactgg gaaatattgt tttcgggtat
     3601 acaagggacc agaaacccgt cagagcaaag gatctgcagg ctcacggtgc gatgaccgtg
     3661 ctgctcaagg acgccctcgc gccgaacctc gtccagaccc tcgagaacaa ccccgccttc
     3721 gtgcacggcg ggccgttcgc caacatcgcg cacggctgca actcggtg
//
LOCUS       DASYJA010000147         3947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2566494, whole genome shotgun sequence.
ACCESSION   DASYJA010000147 DASYJA010000000
VERSION     DASYJA010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2566494"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..1233
                     /locus_tag="VGO02_03110"
     CDS             190..1233
                     /locus_tag="VGO02_03110"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEV7476707.1"
                     /translation="MRLRSLAVAGAMALALAAAGAALAQQKIDMKLAYFVGDQHAMSQ
                     WLIKWSEGLEKGSGGRIAVKRFPSAQMGPTPGHYDFARTGQADVAWFLHGGTPGRFPL
                     TEIINLPFMAGSAEIGTKVINDATLRAKYLDAEHKGIKVLMLFTHQPGGPHTTKKAIR
                     NLDDFKGLRLRFASPTVRDLVGALGATPVGVPPTEISEQLQKGTIDGAFMDYGGVGIA
                     FKLGGIVRYSTELYVYVTSFGLGMNEDYWAKLPPDLKKLVTDSVTGKEKEVGEAWDSL
                     DVPGKKAVMDGGGEAIRFSGPEMEKVRKIGAEVSEAKVKEYDGKGMPGRAVYDQMRGL
                     ADKHSKTSKNFWN"
     gene            1263..1691
                     /locus_tag="VGO02_03115"
     CDS             1263..1691
                     /locus_tag="VGO02_03115"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEV7476708.1"
                     /translation="MAGVFLAAMMLLTVVDVCLRAAFNLPLRGTYEIIELLLACTFFL
                     ALPAVFLRDENIVVDMVDGLAPRAVPLLKRLAEAIAVAVLAVMAWQGWQAAAETLVFN
                     DVTSDLSIHRIVYWIPVLIGMIGAAIAAAMVFFLPENKKP"
     gene            1688..2992
                     /locus_tag="VGO02_03120"
     CDS             1688..2992
                     /locus_tag="VGO02_03120"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEV7476709.1"
                     /translation="MSAGIVGILGVLGLLALIFARVPIAVALAASGLCGYAALDGWGT
                     ALKMFGRVPFTLASAYSLSVIPLFILMGAVASRGNMAKELFEACNGLFSGVRGALANA
                     TIGASALFGAICGSSIATAATFSRVSIPEMRRHGYEASFAAGAVASAGTLAVLIPPSV
                     LLAVYAIVAEQSLPKLYAAAFIPGFVLAGLYVLAVFAVAWWRPKWVPKVPAMRLAQRL
                     RAAAGMWKLGVLFFFSVFGIYLGWFSPTEAAALAAFVAILIGMLTGAMGWRGLWEALL
                     ETAYTTAAIFFIVVGAFIFSRFIVLTQFPNELAAWVKASGLSPAWVLLAVIALYFVLG
                     TFLDEVSTILVTVPVTLPLVLGLGYDGVWFGIFVTVMCTIGLISPPTGMTVFVIQAQH
                     PDIPVVRIYAGTLPYLVADFVLVGLLIAAPGLVSFLPNILRI"
     gene            complement(2989..3945)
                     /gene="galE"
                     /locus_tag="VGO02_03125"
     CDS             complement(2989..3945)
                     /gene="galE"
                     /locus_tag="VGO02_03125"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV7476710.1"
                     /translation="MGSHTCVLLAEAGHELVVVDNLSNAKPVVLERLRELTGRQIEFH
                     RADLRERDALDATFRKPVDAVIHFAGLKAVGESVEKPQLYHENNVGGSVNLLAAMAAH
                     GTRQIVFSSSATVYGDPQRLPLTEDHPLRPTNPYGENKAEIERLLVASSVKYAALRYF
                     NPIGAHASGRIGEDPRGIPNNLMPYVAQVAVGKLPALRVWGNDYPTADGTGVRDYIHV
                     TDLARGHLAALEFLGRKKRSITANLGTGSGYSVLEAVQAFERASGKTVPLEFHPRRPG
                     DVAASYADASLAARELGWKAALGLDDMCRDAWRWQSQNPDGY"
BASE COUNT          561 a         1256 c         1427 g          703 t
ORIGIN      
        1 ccagaagcat gcgacgcaac atctgaactc cctcccctag aggaagtggc aattttagcc
       61 ccgacttagg gtggaaagcc gccaaagagt gcgaggagtc ggggctaaac ccttggtaat
      121 cattaccgat ggcgggtcta gcccggtgtc cgggttatat ttgccccacg aattcctgag
      181 gaggggtgca tgaggctgag gagcttggcc gtggcaggcg cgatggcgct tgcgctggcg
      241 gccgcggggg ctgcgctggc gcagcagaag atcgacatga agctggcgta cttcgtgggc
      301 gaccagcacg ccatgtcgca gtggctgatc aagtggtcgg agggcctcga gaagggctcg
      361 ggcgggcgca tcgcggtgaa gcgctttccg tcggcgcaga tggggccgac gccggggcac
      421 tacgactttg cgcgcaccgg gcaggcggac gtcgcctggt tcctgcacgg cggcacgccg
      481 ggtcggtttc cgctcacgga gatcatcaat ctgccgttca tggccggcag cgcggagatc
      541 ggcaccaagg tgatcaacga cgccacgctg cgcgcgaagt atctcgacgc cgagcacaag
      601 gggatcaagg tgctgatgct gttcacgcac cagcccggcg ggccgcacac gacgaagaag
      661 gcgatccgca acctcgacga cttcaagggg ctgcgcctgc gcttcgcctc gccgacggtg
      721 cgcgacctgg tgggggcgct cggcgcgacg ccggtgggcg tgccgccgac ggagatctcg
      781 gagcagctgc agaagggcac catcgacggc gcgttcatgg actacggcgg cgtcggcatc
      841 gcgttcaagc tgggcgggat cgtcaggtac tcgaccgagc tctatgtcta cgtcacgagc
      901 ttcggcctgg ggatgaacga ggactactgg gcgaagctgc cgcccgacct gaagaagctg
      961 gtcaccgaca gcgtcaccgg caaggagaag gaagtgggcg aggcctggga ctcgctcgac
     1021 gtgccgggca agaaggcggt gatggacggc ggcggcgagg cgatccgctt ctcgggtccg
     1081 gagatggaga aggtgcgcaa gatcggcgcc gaggtgtcgg aggccaaggt gaaggagtac
     1141 gacggcaagg gcatgccggg gcgcgcggtg tacgaccaga tgcgcgggct cgccgacaag
     1201 cactcgaaga cgtcgaagaa cttctggaac tgaagaagag aattgccgcg gtctgcggcg
     1261 gggtggcggg ggtcttcctc gccgccatga tgctgctcac ggtggtggac gtctgcctgc
     1321 gcgcggcgtt caacctgccg ctgcgcggca cctacgagat catcgagctg ctgctcgcct
     1381 gcacgttctt cctcgcgctg ccggcggtgt tcctgcgcga cgagaacatc gtggtggaca
     1441 tggtggacgg cctggcgccg cgcgcggtgc cgctgctcaa gcgcctggcg gaggcgatcg
     1501 ccgtcgcggt gctggcggtg atggcgtggc agggctggca ggcggcggcc gagacgctgg
     1561 tcttcaatga cgttacctcc gacctgtcga tccaccgcat cgtgtactgg attccggtcc
     1621 tgatcgggat gatcggcgcc gcgatcgctg cggcaatggt ttttttcctt ccggaaaaca
     1681 aaaaaccttg agtgccggaa tagtcgggat cctcggcgtg ctcgggctgc tcgcgctgat
     1741 cttcgcgcgc gtgccgatcg cggtggcgct cgccgcgagc ggcctgtgcg gctacgccgc
     1801 gctcgacggc tggggcacgg cgctcaagat gttcggccgg gtgccgttca cgctggcgag
     1861 cgcgtactcg ctgtcggtga ttccgctgtt catcctgatg ggcgcggtgg cctcgcgcgg
     1921 caacatggcg aaggagctgt tcgaggcgtg caacggcttg ttctcgggcg tgcgcggcgc
     1981 gctcgccaac gcgaccatcg gcgcgagcgc gttgttcggc gcgatctgcg gctcctcgat
     2041 cgccaccgcg gcgaccttct cgcgggtctc gattcccgag atgcggcgcc acggctacga
     2101 agcgtcgttc gccgccggcg cggtggcgag cgccggcacg ctcgcggtgc tgatcccgcc
     2161 ctccgtgctg ctcgcggtct acgccatcgt cgccgagcag tcgctgccca agctctacgc
     2221 tgccgcgttc atccccggct tcgtgctcgc cggcctctat gtgctcgcgg tgttcgcggt
     2281 ggcgtggtgg cggccgaagt gggtgccgaa ggtgccggcg atgcgcctcg cgcagcgact
     2341 gcgcgccgcg gccggcatgt ggaagctggg cgtgctgttc ttcttctcgg tgttcggcat
     2401 ttacctcggc tggttctcgc cgaccgaggc ggcggcgctc gccgccttcg tcgcgatcct
     2461 gatcggcatg ctcaccggcg ccatgggctg gcgcgggctg tgggaggcgc tgctcgagac
     2521 cgcctacacc accgcggcga tcttcttcat cgtggtgggc gcgttcatct tctcgcgctt
     2581 catcgtgctg acgcagttcc cgaacgagct cgccgcctgg gtcaaggcct cgggcctctc
     2641 gccggcgtgg gtcctgctcg cggtgatcgc gctgtacttc gtgctcggca ccttcctgga
     2701 cgaggtcagc accatcctgg tcaccgtgcc ggtgacgctg ccgctggtcc tcggcctggg
     2761 ctacgacggc gtctggttcg gcatcttcgt caccgtgatg tgcaccatcg gcctgatcag
     2821 cccgccgacc ggcatgacgg tgttcgtcat ccaggcgcag caccccgaca tcccggtggt
     2881 gcgcatctat gcggggacgc tgccgtatct cgtggcggac ttcgtgctgg tggggctgct
     2941 cattgcggcg ccgggtctgg tttcgttttt accaaatatt ttaagaatct aatatccatc
     3001 cgggttttgc gactgccagc gccaggcatc gcggcacatg tcgtccaggc cgagcgcggc
     3061 tttccagccg agctcgcgcg cggcgagcga cgcgtcggcg taggaggcgg cgacgtcgcc
     3121 ggggcggcgc gggtggaact cgagcggcac cgtcttcccc gaggcgcgct cgaaggcctg
     3181 caccgcctcg agcaccgagt agccgctgcc ggtgccgagg ttggcggtga tcgagcgctt
     3241 cttcctgccg aggaactcca gcgcggcgag gtggccgcgg gcgaggtccg tgacgtggat
     3301 gtagtcgcgc acgccggtgc cgtccgccgt cggatagtcg ttgccccaga cgcgaagcgc
     3361 cggcagcttg ccgaccgcga cctgcgcgac gtagggcatg aggttgttcg ggatgccgcg
     3421 cgggtcctcg ccgatccggc cggaggcatg cgcgccgatc ggattgaaat agcgcagcgc
     3481 ggcgtacttc acgctgctcg ccaccagcag ccgctcgatc tcggccttgt tctcgccgta
     3541 gggattggtc gggcgcagcg ggtggtcctc ggtgagcggc aggcgctgcg ggtcgccgta
     3601 gaccgtggcc gaggagctga agacgatctg ccgcgtgccg tgcgccgcca tcgccgcgag
     3661 caggttcacg ctgccgccga cgttgttctc gtggtagagc tgcggcttct cgaccgactc
     3721 gccgaccgcc ttcaggccgg cgaagtggat caccgcgtcg accggcttcc tgaaggtggc
     3781 gtcgagcgcg tcgcgctcgc gcaggtcggc gcggtggaac tcgatctgcc ggccggtcag
     3841 ctcgcgcagg cgctcgagca ccaccggctt ggcgttcgac aggttgtcga ccacgaccag
     3901 ctcgtgtccg gcctcggcga gcagcacgca ggtatgcgac ccgatgt
//
LOCUS       DASYJA010000148         1606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2929624, whole genome shotgun sequence.
ACCESSION   DASYJA010000148 DASYJA010000000
VERSION     DASYJA010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2929624"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..1446
                     /gene="rho"
                     /locus_tag="VGO02_03130"
     CDS             235..1446
                     /gene="rho"
                     /locus_tag="VGO02_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEV7476711.1"
                     /translation="MAVQHEIEGANRLRKQELIFALLKNRAKKGESIFGDGTLEVLPD
                     GFGFLRSPETSYLASTDDIYVSPSQIRRFNLHTGDSIEGEIRTPKDGERYFALVKVDK
                     VNAEAPEAAKNKILFENLTPLHPDKAFKLEREIKAEENLTGRVLDIVAPIGKGQRGLI
                     VSPPKAGKTVMLQHIAHALVANHPEITLIVLLIDERPEEVTEMSRTVRGEVVASTFDE
                     PASRHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVVPASGKVLTGGVDAN
                     ALQKPKRFFGAARNIEEGGSLTIIATALIDTGSRMDDVIYEEFKGTGNLEVHLDRRMH
                     EKRIYPAINVNRSGTRREELLIEQPILQKVWVLRKLLFPMDELEAAEFLIDKLRATKS
                     NSEFFDSMRRG"
BASE COUNT          339 a          542 c          492 g          233 t
ORIGIN      
        1 ccccctccat cccggagggg ggtgaatcca acaaggtcgt caagcgccgc tccggcgcag
       61 aagttctacg tatctcccaa catgagcagg tcaccagcgg cacagcccga agcagtccgt
      121 caagcccaag gcgaggatgc ggcgcccgcg cccgcgccgg cagcagctag ccccgtcgcg
      181 atccccacgc atctctcgga gctgaagtcg catcacgttt cgcagctcgt cgacatggcc
      241 gtgcaacacg aaatcgaagg cgccaaccgc ctgcgcaagc aggagttgat cttcgcactg
      301 ctgaaaaacc gcgcgaaaaa aggcgaatcg attttcggcg acggcacgct cgaagtgctg
      361 cccgacggct tcggtttcct gcgctcgccg gagacctcct acctcgcgag caccgacgat
      421 atctacgtct cgccctcgca gatccggcgc ttcaacctgc acaccggcga ctcgatcgaa
      481 ggcgagattc gcacgcccaa ggacggcgag cgctacttcg cgctcgtcaa ggtcgacaaa
      541 gtcaacgccg aggccccgga ggcggccaag aacaagatcc tgttcgagaa cctcacgccg
      601 ctgcatccgg acaaggcgtt caaactcgag cgcgagatca aggcggagga gaacctcacc
      661 ggacgcgtgc tggacatcgt cgcgcccata ggcaagggcc agagagggct gatcgtcagc
      721 ccgcctaagg ccggcaagac ggtgatgctg cagcacatcg cgcacgccct ggtcgccaac
      781 cacccggaga tcacgctgat cgtgctgctg atcgacgagc gacccgagga agtgacagag
      841 atgtcgcgca ccgtgcgtgg cgaagtggtc gcctctacct tcgacgagcc cgcctcgcgc
      901 cacgtgcagg tcgccgagat ggtgatcgag aaggcgaagc gcctggtcga gcacaagaag
      961 gacgtggtga tcctgctcga ctcgatcacc cgcctcgcgc gggcctacaa caccgtcgtc
     1021 cccgcgtcgg ggaaggtcct gacaggcggc gtcgacgcaa atgcgctgca gaagcctaag
     1081 cgcttcttcg gtgcggcgcg caacatcgag gaaggcggct cgctcaccat catcgccacc
     1141 gccctcatcg acaccggcag ccgcatggac gacgtgatct acgaggagtt caagggcacc
     1201 ggcaacctgg aggtgcacct cgaccgccgc atgcacgaga agcgcattta cccggcgatc
     1261 aacgtcaacc gctcaggcac ccgccgcgag gagctgctga tcgagcagcc gatcctgcag
     1321 aaggtctggg tgctgcgcaa gctcctgttc cccatggacg agctcgaggc ggccgagttc
     1381 ctgatcgaca agctgcgagc cacgaagtcg aattccgagt tcttcgattc gatgcgccgg
     1441 ggctgaaagc acgaacctaa aaaagcagta aaaatcgggg gatgctcgac accacgcgca
     1501 aggtggcgac gcccgaaggc atcgagctca ccctgcgtct cgccggaccg gtgccgcgcg
     1561 cgatggcctg ggcggtcgac ctcgcgatcc gcgccgcaat cgtgtt
//
LOCUS       DASYJA010000149         3993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2839715, whole genome shotgun sequence.
ACCESSION   DASYJA010000149 DASYJA010000000
VERSION     DASYJA010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2839715"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VGO02_03135"
     CDS             complement(<1..184)
                     /locus_tag="VGO02_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEV7476712.1"
                     /translation="MLYELRVYRCIPGRLPALHKRFQEATLGIWERHGIRQVGFWTVV
                     IGDGNHDLYYLLEWKDM"
     gene            207..1364
                     /locus_tag="VGO02_03140"
     CDS             207..1364
                     /locus_tag="VGO02_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEV7476713.1"
                     /translation="MKRIPLERVEAGLPAHWYYDDAQYKRELEAFWYRGWIAACRDEE
                     LPSPGDWRVASIGSQKIILLRNEKGELRAFHNTCRHRGSILCEKESGRFARGRIVCPY
                     HSWTYDLDGRLAGTPRRMETPDFDAKDFPLFGVSVDTWGGFVFVNLAAKPPPLAKRTA
                     GLQEKLANYDFEKLRIGKRIVVEVKANWKLLVENFAECFHCPPVHPELCRVVTAYQEA
                     GAWGLAERFSSKPEFKPGATTLTMDGTSCLAAFSGLNGEEKRKIYVPEMLPPNLFLNI
                     QPDYVNSHLMFPTGPQSVRIVYDWLFEPRHLPLPAADLQHYVALWEITNAQDARNCEW
                     QQQGIHALPFEHGHYVPQEFDAHRFAQWIRKGLGVRGKAASRPRRARKTAR"
     gene            complement(1312..1464)
                     /locus_tag="VGO02_03145"
     CDS             complement(1312..1464)
                     /locus_tag="VGO02_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476714.1"
                     /translation="MPGRIGKSYPRMQSAQAQGAPAPRARGYGMLGGLIALFSALGVV
                     GWLLFL"
     gene            complement(1522..2331)
                     /gene="pssA"
                     /locus_tag="VGO02_03150"
     CDS             complement(1522..2331)
                     /gene="pssA"
                     /locus_tag="VGO02_03150"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEV7476715.1"
                     /translation="MAEDDDNNHDLDHDPEVIPERRSGLGGLRRRGIYLLPNLFTTGV
                     LFCGFFAVVQAINGRFDVAAIAVYVAMVLDGMDGRVARWTNTQSEFGAQYDSIADMVA
                     FGAAPALIAYEWALKDLGTAGWIGAFIYCAAAGIRLARFNANIGVVDKRYFQGMPSPS
                     GAAVLTGFIWAFTELGFVPDESYWLSSLAWVVAVFAGVTMVLNVPFWSFKEVNWRKRV
                     PLWVILACVIGFSVVSFKPPVILFLLFFGYSLSGYVMWAMGHRVRSVLPEE"
     gene            complement(2280..2963)
                     /locus_tag="VGO02_03155"
     CDS             complement(2280..2963)
                     /locus_tag="VGO02_03155"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEV7476716.1"
                     /translation="MRPPYPHPVIAREGWPYLAGAFLFSLLVTFFAGWWSLPFWLLTL
                     FILQFFRDPPREVPDDPRAVVSPADGRIVEVSRVQDPYLKRDALKLSVFMNVFNVHSN
                     RSPVDGVMKERWYFPGAFVNAALDKASLDNERNALWLRTDGGADVTCVQVAGLIARRI
                     LCYVSPGARLRRGERYGFIRFGSRVDVYLPPDAEPAAALGDKVYAAESVLAWLKTTTT
                     TTTSTTTRK"
     gene            complement(2963..3109)
                     /locus_tag="VGO02_03160"
     CDS             complement(2963..3109)
                     /locus_tag="VGO02_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476717.1"
                     /translation="MALVGCTPEQSKAIGNQPKKTVDKVTSDVTKAMQQGGQDSERLK
                     EDQK"
     gene            complement(3126..>3993)
                     /gene="ilvC"
                     /locus_tag="VGO02_03165"
     CDS             complement(3126..>3993)
                     /gene="ilvC"
                     /locus_tag="VGO02_03165"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV7476718.1"
                     /translation="ASWAKAEAAKLKVAEVGEAVKAADIVMMLLPDEQIAAVYKTDVE
                     PNIKKGASLAFAHGFNIHYGLIQERKDLDVWMVAPKAPGHTVRSTYAAGGGVPMLIAV
                     HRNSSKKARELALSYAAAIGGGKAGIIETDFKEETETDLFGEQSVLCGGCVELVKAGF
                     DTLVDAGYAPEMAYFECLHELKLIVDLMYEGGIGTMNYSISNNAEYGEYVSGPKVIDA
                     GTRARMKEILHRIQTGEYAKEFVLENRAGAPTLLSRRRMTAELPIEKVGARLREMMPW
                     IKRNRLVDTSKN"
BASE COUNT          726 a         1314 c         1286 g          667 t
ORIGIN      
        1 ccatgtcctt ccactccagc aggtagtaca ggtcgtggtt gccatcgccg attaccacgg
       61 tccagaagcc gacctggcgg atgccgtgcc gctcccagat gccgagcgtg gcctcctgga
      121 atcgtttgtg cagggcaggc aggcggcccg ggatgcagcg gtagacgcga agctcgtaaa
      181 gcatgctttc tccctaagat agcggcatga aacgcatacc gctcgagcgg gtcgaggcgg
      241 ggctgccagc gcactggtac tacgacgacg cgcagtataa gcgcgagctg gaggccttct
      301 ggtaccgcgg ctggatcgct gcctgccgcg acgaggagct tccctccccc ggtgactggc
      361 gggtggcatc gatcggttcc cagaaaatca ttcttctgag aaacgaaaaa ggcgagctgc
      421 gcgcctttca caacacctgc cgccaccgcg gctcgatcct ttgcgaaaag gaaagcggca
      481 ggttcgcccg cggccgcatc gtctgcccct accactcctg gacctacgac ctcgacggcc
      541 gcctggccgg cacgccgcgg cgcatggaaa caccggactt cgatgcgaag gactttccgc
      601 tgttcggggt ctcggtcgac acctggggcg gcttcgtttt cgtgaacctc gccgccaagc
      661 ccccgccgct cgcgaaacgg accgcagggc tgcaggagaa actggcgaac tacgactttg
      721 aaaagctgcg catcggcaag cgcatcgtgg tcgaggtgaa ggcgaactgg aagctgctgg
      781 tggagaactt cgccgaatgc ttccactgcc cgccggtgca cccggagctg tgccgggtcg
      841 tcaccgcgta tcaggaagcc ggcgcgtggg gcctggcgga gaggttttca tccaagcccg
      901 agttcaagcc aggcgcgacg acgttgacga tggacggcac ttcgtgcctt gcggcatttt
      961 ccggtctgaa tggagaagaa aaacgcaaga tctacgttcc cgagatgctg ccacccaacc
     1021 tgtttctcaa tatccagccc gactacgtca actcgcacct gatgttcccg accgggccgc
     1081 agagcgtgcg catcgtgtac gactggctgt tcgagccgcg gcacctgccg ctgcccgcag
     1141 cggatcttca gcactacgtc gcgttatggg aaatcaccaa cgcgcaggac gcgcgcaact
     1201 gcgagtggca gcagcagggc atccacgccc tgcccttcga gcatggccac tatgtgccgc
     1261 aggaattcga cgcccaccgc ttcgcgcagt ggatcaggaa agggcttggc gtcagaggaa
     1321 aagcagccag ccgaccacgc cgagcgcgga aaacagcgcg atgagcccgc ccagcatgcc
     1381 gtagccgcgc gcgcgcggcg ccggcgcgcc ttgcgcctgc gcggactgca ttcgcggata
     1441 gctttttccg atgcggccgg gcatacgggg atcatgagcc ggtccctcgc cgccgggaag
     1501 cggcttgaac aaatttgact cttactcttc cggaagcacc gagcgcacgc ggtggcccat
     1561 tgcccacatg acatatccgg acaacgagta gccgaagaag agcaggaaca aaatcacggg
     1621 gggcttgaac gagaccaccg aaaagccgat gacgcaggcg aggatcaccc aaaggggcac
     1681 gcgcttgcgc cagttcactt ccttgaagct ccaaaagggc acgttcagca ccatggtcac
     1741 cccggcgaac accgccacta cccaggcgag gctggagagc cagtaggact cgtcgggcac
     1801 gaagccgagc tcggtgaagg cccagatgaa gccggtcagc accgccgccc cggagggact
     1861 cggcataccc tggaagtagc gcttgtcgac cacgccgatg ttggcgttga agcgcgcgag
     1921 ccggatgccg gccgcggcgc agtagatgaa ggcgccgatc cagccggcgg tgccgaggtc
     1981 cttgagcgcc cactcgtagg cgatgagcgc cggcgccgcg ccgaaggcga ccatgtcggc
     2041 gatgctgtcg tactgcgcgc cgaactcgct ctgcgtgttg gtccagcgcg ccacgcgccc
     2101 gtccatgccg tcgagcacca tggcgacgta gacggcgatg gctgcgacgt cgaagcggcc
     2161 gttgatcgcc tgcaccacgg cgaagaagcc gcagaacagg accccggtgg tgaacagatt
     2221 cggcagcagg tagatgccgc gccggcgcag gccgcccaga ccgctgcgcc gctccggaat
     2281 tacttccggg tcgtggtcga ggtcgtggtt gttgtcgtcg tcttcagcca tgcgagcacg
     2341 ctttcggcag cgtacacctt gtcgccgagg gccgctgccg gctcggcgtc gggcggcagg
     2401 tagacgtcga cccgcgagcc gaagcggatg aagccgtagc gctcgccgcg gcgcagcctg
     2461 gcgccgggcg agacatagca aagaatgcgc cgcgcgatca ggccggcgac ctgcacgcag
     2521 gtgacgtcgg cgccgccgtc ggtacgcagc cacagcgcgt tgcgctcgtt gtcgagcgaa
     2581 gccttgtcga gcgcggcatt gacgaacgcc cccggaaagt accagcgctc cttcatcacg
     2641 ccgtcgaccg gcgagcggtt cgagtgcacg ttgaagacgt tcatgaacac gctcagcttg
     2701 agcgcgtcgc gcttgaggta ggggtcctgc acccggctca cctcgacgat gcggccgtcg
     2761 gccggtgaca caaccgcgcg cgggtcgtcg ggcacctcgc gcggcgggtc acggaagaac
     2821 tgcaggatga acagggtgag cagccagaat ggcagcgacc accatcctgc gaagaaggtt
     2881 accaggagag agaaaagaaa ggcgccggcg aggtacggcc acccctcccg cgcgatgacc
     2941 ggatgcggat acggcggccg cactacttct ggtcttcctt caggcgctcg gagtcctgcc
     3001 cgccctgctg catcgccttg gtgacgtcgc tagtgacctt gtcgaccgtc ttcttcggct
     3061 ggttgccgat cgccttggac tgttccgggg tacagccgac aagcgccatc gaaaaaatca
     3121 agatgctagt tttttgaagt atcgacaagg cggttccttt ttatccaggg catcatctcg
     3181 cgcagccgcg ccccgacctt ctcgatcggc agctcggcgg tcatgcgccg gcgcgaaagc
     3241 agcgtcggcg cgccggcacg gttctccagc acgaactcct tggcgtactc gcccgtctgg
     3301 atgcggtgca gaatctcctt catgcgcgct cgcgtgcccg cgtcgatcac cttgggcccg
     3361 gagacgtatt cgccgtattc cgcgttgttc gagatcgagt agttcatggt gccgatgccg
     3421 ccctcgtaca tcaggtcgac gatcagcttg agctcgtgca ggcactcgaa gtaggccatc
     3481 tcgggggcgt agccggcgtc gaccagcgtg tcgaagcccg ccttcaccag ctcgacgcag
     3541 ccgccgcaca gcacgctctg ctcgccgaac aggtcggtct cggtctcttc cttgaaatcg
     3601 gtttcgatga tgccggcctt gccgccgccg atcgccgccg cgtaggacag cgcgagctcg
     3661 cgcgccttct tcgaggaatt gcggtgcacc gcgatcagca tcggcacgcc gccgcccgcc
     3721 gcgtaggtcg aacggaccgt gtggccgggg gccttgggcg cgaccatcca cacgtcgagg
     3781 tccttgcgct cctggatcag gccgtagtgg atgttgaacc cgtgcgcgaa ggcgagcgac
     3841 gctcccttct tgatgttcgg ctcgacgtcg gtcttgtaga cggccgcaat ctgctcgtcg
     3901 ggcaacagca tcatcacgat gtcggccgcc ttcaccgcct cgccgacctc ggccaccttg
     3961 agcttggccg cctcggcctt ggcccacgag gct
//
LOCUS       DASYJA010000150         5479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_842407, whole genome shotgun sequence.
ACCESSION   DASYJA010000150 DASYJA010000000
VERSION     DASYJA010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842407"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VGO02_03170"
     CDS             complement(<1..463)
                     /locus_tag="VGO02_03170"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEV7476719.1"
                     /translation="MSRSLLSLVLLAAALPACSWVPRIPGVTPYRIEVQQGNFVSQEM
                     LSQLKPGMSKEQVRLTMGTPLLADAFHADRWDYIYTREAPNGKRESRKLVVFFADNKL
                     ARVDGDGVQLERPKDQPVPPPATQAPVAPATQTAAPAAAPPVGQDAAAPAVP"
     gene            483..917
                     /gene="fur"
                     /locus_tag="VGO02_03175"
     CDS             483..917
                     /gene="fur"
                     /locus_tag="VGO02_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006682173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEV7476720.1"
                     /translation="MSTVQSLKDIGLKATLPRRKILELFEASKVRHLSAEDVYRALIA
                     EGIDVGLATVYRVLTQFEQAGLLSRQNFEAGKAVFELNQGGHHDHLVCLQCGRVEEFY
                     DGEIEKRQNEVARQRGFALHGHSLALYADCVKKDCPHRKSTL"
     gene            complement(914..2554)
                     /gene="recN"
                     /locus_tag="VGO02_03180"
     CDS             complement(914..2554)
                     /gene="recN"
                     /locus_tag="VGO02_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEV7476721.1"
                     /translation="MLRAIDIRDFVIVEQAGLEPGAGFTVLTGETGAGKSILVDAIEL
                     LVGGRGDGGLVREGAERAELSAEFDVDSQKELAKWISEHDLAGDPDRLILRRTIDRTG
                     RSRCFVNGHAATLAQLREAGEFLVDIHGQHEHQSLLRPAAQRSLLDTHAAAQELARET
                     AEACRAWKRLQEVAAETQRNFAAREAERAELRERAGELKKLAPREGEWQQVSAEQTRL
                     SHGSALLAGAQSSLEALAEAEGACLAQLSAIAAQLRGLSAHDARLGAIVEMLESAEAQ
                     AGEAARELRHYASRIDLDPQALKQTETRIEALHAAGRKFRVRPEALHELLRELEKRLE
                     ELELAVDPAALEREVAAARARFEALLKKLTARRRSAADALSRAVSAAMQQLAMAGGRF
                     SVALKELKEPGTAGAEDIEFEVSSHPSLPLRPLAKVASGGELSRISLAIQLVAAKDSP
                     VGTLVFDEVDSGMGGAVAETIGKSLRRLGKARQVLCVTHLPQVAANGDAQWGVTRSGI
                     KVKVEKLDREARIAELARMLGGSEKTARKHAAELLDAG"
     gene            complement(2558..3394)
                     /locus_tag="VGO02_03185"
     CDS             complement(2558..3394)
                     /locus_tag="VGO02_03185"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEV7476722.1"
                     /translation="MKPSFRRIALIGKPSSPEIEGSLREMQRFLEQRGCEVLKPGDTK
                     ADLAIVIGGDGTMLAAARNLVRHGVALIGVNQGRVGFMTDIGRDDMQAGIGAVLDGKF
                     LIEERSLLDAEILREGKSVLRTLALNEAVVGKGAQGRLIEFELSIDGEFVYALRADGV
                     IVATPTGSTAYAMSAQGPILHPAVPALALVPLNPHTLSARPVSVSDTSRIEISLVRAV
                     DARAHFDGFALTDMQEGDRLTLKRSADTVRFVHPPGYRYFATLREKLGWSESIEKDRG
                     LG"
     gene            3448..4467
                     /gene="hrcA"
                     /locus_tag="VGO02_03190"
     CDS             3448..4467
                     /gene="hrcA"
                     /locus_tag="VGO02_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEV7476723.1"
                     /translation="MLDKRAQLLLKTLIERYIAEGQPIGSRTLSKYSGLDLSPATIRN
                     VMSDLEEMGLISSPHTSAGRIPTPLGYRFFVDTLLVIKQIESGELRELEGQLHPDNPQ
                     RVIQAASQILSQLTQFAGVVMTPKRRSLSFRHVEFLRLSDKRILLIVVTPEGDVQNRI
                     LYTERDYASGELVEAANFMNQNYSGQSFEDMRTLLQRELSELRQDMIQLMTAAVDAGS
                     EALAQQSEPYVITGERNLLGARDLSQDMSRLKQLLELFERKTSLVQILDVSLRGQGVQ
                     IFIGGESGFSAPDDVSVVTSPYKVDGEVVGTVGVIGPTRMAYDRVIPIVDVTAKLLSS
                     ALSQH"
     gene            4473..5357
                     /gene="hemH"
                     /locus_tag="VGO02_03195"
     CDS             4473..5357
                     /gene="hemH"
                     /locus_tag="VGO02_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEV7476724.1"
                     /translation="MLLVNLGTPAAPTEAAVRSYLAEFLSDPRVVRLPRFVWLPILHG
                     VVLRKRPALSAARYAAIWTPEGSPLAVHTRRQAAALQILLADTKVEYAMRYGEPSLAA
                     ARVRLPEARVVPLYPQYSDSTTGSIIDVMGPGQQIVMQYYDHPAYIDALAANVRRHWQ
                     AHGRGAKLLMSFHGLPRRGGALYAGQCYGTSKLLADKLQLGAGEWLTTFQSRFGFAEW
                     LQPYTAPTLVSLAQQKVERVDVICPGFVADCLETLEEIGIEARRAYITAGGREFHLIA
                     CLNESPEWIAALARIAAS"
BASE COUNT          816 a         1938 c         1852 g          873 t
ORIGIN      
        1 gcggtacggc gggcgctgcg gcatcttgcc ccacgggtgg cgccgccgcc ggcgctgcgg
       61 tctgcgtggc gggcgctacg ggagcttgcg tcgctggcgg cggcacgggc tggtccttag
      121 gccgctcaag ctgaaccccg tcgccgtcga cgcgcgccag cttgttgtcg gcgaagaaca
      181 ccaccagctt gcgcgactcg cgcttgccgt tcggcgcttc gcgcgtgtag atgtagtccc
      241 agcgatcggc gtggaaagcg tcggcgagca gcggcgtgcc catggtgagg cgcacctgct
      301 ccttgctcat gccgggcttg agctgcgaca gcatttcctg cgagacgaaa tttccctgct
      361 gcacttcgat cctgtagggc gtgacgccgg gaatgcgcgg cacccagctg cacgccggca
      421 gcgccgcggc gaggaggacc agagagagga gcgatcgtga catgggcata taatgacagc
      481 cgatgagcac agtgcaaagc ctgaaggaca tcggtctcaa ggcaacgctg ccgcggcgca
      541 agatcctgga gctgttcgag gcgagcaagg tgcgccattt gtccgccgag gacgtgtaca
      601 gggcgcttat cgccgaaggc atcgacgtcg gcctcgccac ggtctaccgc gtgctcaccc
      661 agttcgagca ggcgggcctg ctgtcgcggc agaacttcga ggccggcaag gcggtgttcg
      721 agctcaacca gggcggccac cacgaccacc tggtgtgcct gcagtgcggc cgcgtcgagg
      781 agttctacga cggcgagatc gagaagcggc agaacgaggt ggcgcgccag cgcggcttcg
      841 cgctgcacgg ccattctctc gcgctgtacg ccgactgcgt caagaaagac tgcccgcacc
      901 gcaagtcgac gctctagccc gcgtccagca gctccgcggc gtgcttgcgc gcggtcttct
      961 ccgagccccc gagcatgcgt gccagctcag cgatcctcgc ttcgcggtcg agtttttcga
     1021 ctttgacctt tatcccggaa cgcgtgacgc cccactgagc gtcgccgtta gcggcgacct
     1081 gcggcaggtg ggtcacgcac agcacctggc gcgccttgcc cagtcgccgc agggacttgc
     1141 cgatggtctc ggctaccgcc ccgcccatgc ccgaatccac ctcgtcgaac accagcgtgc
     1201 cgaccggcga gtccttcgcc gcgaccagct ggatggcaag gctgatgcgc gacagttcgc
     1261 cgccggaggc caccttggcg agcggccgca gcggcaggct gggatgggag gacacctcga
     1321 actcgatgtc ttcggcgccc gcggtgcccg gctccttcaa ttcctttagg gcaaccgaaa
     1381 accggccgcc agccatcgcg agctgctgca tcgccgcgct caccgcgcgc gacagcgcat
     1441 cggcggccga gcgtcgcctg gcggtaagct tcttcagcag ggcctcgaat cttgcgcgcg
     1501 ccgcggcgac ctcgcgctcg agcgccgcgg ggtcgaccgc cagctcgagc tcttcgagcc
     1561 tcttctccag ctcgcgcagc aattcgtgca gcgcttccgg cctgacgcgg aacttgcggc
     1621 cggcggcgtg cagcgcctcg atgcgcgttt cggtctgctt cagcgcctgc ggatcgaggt
     1681 cgatgcgcga agcgtaatgg cgcagctcgc gcgcggcctc gccggcctgc gcctcggccg
     1741 attcgagcat ctcgacgatc gcgccgaggc gcgcgtcgtg cgccgagagc ccgcgcagct
     1801 gcgccgcgat cgccgacagc tgcgcaaggc aggcgccctc ggcttccgcg agcgcttcca
     1861 gcgaggactg ggcgccggca agcagcgccg agccgtgcga caggcgcgtc tgctcggcgc
     1921 tcacctgctg ccattcgccc tcgcgcggcg cgagcttctt cagctcgccg gcgcgctcgc
     1981 gcagctcggc gcgctcggct tcgcgcgcgg cgaagttcct ctgcgtctca gccgcgacct
     2041 cctgcaggcg cttccaggcg cggcacgcct cggccgtctc gcgggccagc tcctgcgctg
     2101 cggcgtgcgt gtcgagcagg ctgcgctgcg ccgccgggcg cagcagtgac tggtgctcgt
     2161 gctggccgtg gatgtcgacc aggaactcgc ccgcctcgcg cagctgggcg agggtcgccg
     2221 cgtgcccgtt gacgaagcag cgcgagcgtc ccgtgcggtc gatggtcctt ctcaggatga
     2281 ggcgatccgg atcgccggcg aggtcatgct cgctgatcca ctttgccagc tccttttgag
     2341 aatccacgtc gaactccgcg gacagctcgg cccgctcggc gccctcgcgc accaggccgc
     2401 cgtcaccgcg gccgcccacc agcagctcga tggcgtccac caggatcgac ttgccggcgc
     2461 cggtctcgcc ggtgagcacg gtgaagccgg cgccgggctc caggcccgcc tgttcgacga
     2521 tgacgaaatc ccggatgtcg atcgcgcgca gcaccgccta tcccagtccg cggtccttct
     2581 cgatgctctc gctccagcct agcttctcgc gcagcgtggc gaagtagcgg tagccgggcg
     2641 ggtgcacgaa gcgcaccgtg tcggccgagc gcttgagcgt caggcggtcg ccttcctgca
     2701 tgtcggtcag cgcgaagccg tcgaagtgtg cgcgcgcgtc gaccgcgcgc accagggaaa
     2761 tctcgatgcg gctcgtgtcg ctgacgctga ccgggcgggc cgagagcgta tgcggattga
     2821 gcggcaccag cgcgagcgcc ggaacggcgg gatgcaggat cggcccctgc gccgacatgg
     2881 cgtaggcggt cgagccggtg ggcgtggcga cgatgacgcc gtcggcgcgc agcgcgtaga
     2941 cgaactcgcc gtcgatcgac agctcgaact cgatcaggcg gccctgcgcg cccttgccga
     3001 ccaccgcctc gttgagcgcc agcgtgcgca gcaccgactt gccctcgcgc aggatctcgg
     3061 cgtcgagcag cgagcgctcc tcgatcagga acttgccgtc gagcaccgcg ccgatgccgg
     3121 cctgcatgtc gtcgcggccg atgtcggtca tgaagccgac gcggccctgg ttgacgccga
     3181 tcagcgccac gccgtggcgc accaggttgc gcgccgcggc gagcatggtg ccgtcgccgc
     3241 cgatgacgat ggcgaggtcg gccttggtgt cgcccggctt gagaacctcg cagccgcgct
     3301 gctcgaggaa gcgctgcatc tcgcgcagcg agccttcgat ctcggggctg ctgggtttgc
     3361 cgatcagcgc gatgcggcgg aagctcggct tcactgagtc ggctccgctg cggcgacggg
     3421 catatcggcg agtgtacaat gaaatccatg ctcgacaagc gcgcccagct cctgctcaag
     3481 acgctgatcg agcgctatat cgccgagggc cagccgatcg gctcgcgcac gctgtcgaag
     3541 tactccggcc tcgacctgtc gccggcgacg atccgcaacg tgatgtccga cctcgaggag
     3601 atgggcctca tctccagccc gcacacctcg gccggccgca tcccgacgcc gctcggctac
     3661 cgcttcttcg tcgacacgct gctggtcatc aagcagatcg agtccggcga gctgcgcgag
     3721 ctcgaagggc agctgcatcc cgacaatccg cagcgcgtga tccaggccgc ctcgcagatt
     3781 ctctcccagc tcacgcagtt cgccggcgtg gtgatgacgc caaagcggcg cagcctgagc
     3841 ttccggcacg tcgagttcct caggctctcg gacaagcgca tcctgctgat cgtggtcacg
     3901 ccggagggcg acgtgcagaa ccgcatcctg tacaccgagc gcgactacgc ctccggcgag
     3961 ctggtcgagg cggccaactt catgaaccag aactactcgg gccagagctt cgaggacatg
     4021 cgcacgctgc tgcagcgcga gctctccgag ctgcgccagg acatgatcca gctgatgacc
     4081 gcggcggtcg acgccggcag cgaggcgctg gcgcagcaga gcgagcctta tgtgatcacc
     4141 ggcgagcgca acctgctcgg cgcgcgcgac ctgtcgcagg acatgagccg gctgaagcag
     4201 ctgctcgagc tgttcgagcg caagacctcg ctggtgcaga tcctcgacgt ctcgctgcgc
     4261 ggccagggcg tgcagatctt catcggcggc gagtccggct tcagcgcccc cgacgacgtc
     4321 agcgtggtca cgtcgcctta caaggtggac ggcgaggtgg tgggcaccgt cggcgtcatc
     4381 gggcccacgc gcatggccta cgaccgcgtc atcccgatcg tcgacgtcac ggcgaagctg
     4441 ctgtcgagcg cgctctcgca acactgaaag aaatcctcct cgtaaacctg ggaacgcccg
     4501 ccgcgccaac cgaagcggcg gtgcgtagct acttggcgga gttcctgtcc gatccgcgcg
     4561 tcgtgcggct gccgcgcttc gtctggctgc cgatcctgca cggcgtggtc ctgcgcaagc
     4621 ggccggccct gtcagcggcg aggtacgcgg cgatctggac tcccgagggc tcgccgctcg
     4681 cggtgcatac ccgcaggcag gccgccgcgc tgcagatcct gctggcggac acgaaggtcg
     4741 agtacgccat gcgctacggc gagccgtccc tcgccgcggc gcgcgtgcgg ctgcccgagg
     4801 cgagggtggt gccgctttac ccgcagtact ccgacagcac cacgggcagc atcatcgatg
     4861 tgatggggcc gggacagcag atcgtgatgc agtattacga ccaccccgcc tatatcgacg
     4921 cgctggccgc caacgtgcgg cggcactggc aggcgcacgg ccgcggcgca aagctgctga
     4981 tgagcttcca cggcctgccg cgccgcggcg gcgccctcta tgccgggcag tgctacggca
     5041 cttcgaagct gctcgccgac aagctgcagc tcggcgccgg cgagtggctg acgaccttcc
     5101 agtcgcgctt cggcttcgcc gaatggctgc agccctatac cgccccgacg ctggtctcgc
     5161 tggcgcagca gaaggtcgag cgcgtggacg tgatctgccc cggcttcgtc gccgactgcc
     5221 tggagacgct ggaggaaatc ggcatcgaag cgcgccgcgc ctacatcacc gccggcggca
     5281 gggaattcca cctcatcgcc tgcctcaacg aatcgcccga gtggatcgcg gcgctggcaa
     5341 gaatcgccgc gtcctgacta gcgcccgagc agccccttca gcttgccgcc gagctgtttc
     5401 tcgacctggt ccttgacctt ctcgcccgcc ttgctcttgg cgaggtcggt cgccaccgcg
     5461 ccgtagttca cgtcgtact
//
LOCUS       DASYJA010000151         3323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1385875, whole genome shotgun sequence.
ACCESSION   DASYJA010000151 DASYJA010000000
VERSION     DASYJA010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385875"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="VGO02_03200"
     CDS             <1..1051
                     /locus_tag="VGO02_03200"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEV7476725.1"
                     /translation="FPAPLWHELDGGRYIGTGCSVVTRDFDSEWINVGTYRVQVIDRN
                     HVALDMVPGKHGRIHYEKYKAAGKRFPVVIVCGADPLGYLISGIEIPFGMCEWNYVGS
                     ILKDPVSVLKGEVTGLPFPAASEIVLEGFVEPNDERTEGPFGEFHGYYPGKAGTAPVV
                     TLERIYFRDSPIIVGSPPAKPPNDYSYSKAVMRSALLADALAAAGVPDVRAVWAHEIG
                     GARMFNVVSIKQRYPGHARQAGHILSQCGVGAYMSRYSVVVDDDIDPSNLQEVMWAVA
                     TRTDPSVDIDIIQRGVGSKNDPMFVAYKYDAPLSSKAVIDACRPWEHLGEFPPVAEAS
                     KELQDRTRSKWKEIL"
     gene            1103..1738
                     /locus_tag="VGO02_03205"
     CDS             1103..1738
                     /locus_tag="VGO02_03205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7476726.1"
                     /translation="MHIETVKAAYRRYAGFYDALFGPVLQPGRRAVLEALQLRPGDRV
                     LEVGVGTGLSLPMYPQTVKVIGIDLSSEMLAKARLRVARRKLANVESLLEMDAESMSF
                     PDASFDKVVAMYVMPVVRDPARVLEELHRVCRPDGEIFIVNHVKSDNRIIRAFEMGLA
                     RFSNQIGFRPDFELRELVNGAGEIDEVARINVFWRVMRVRNGVAHRRLAAR"
     gene            complement(1743..2714)
                     /locus_tag="VGO02_03210"
     CDS             complement(1743..2714)
                     /locus_tag="VGO02_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEV7476727.1"
                     /translation="MRIYLLTGLLVLSNAIQAQGYPARAITLIIPFPPGGSTDIVGRI
                     AAEGLAKELGQPFVIDNRGGAGGAIGAKAIAEAAPDGSTLGLATVSTHVVNPVVVPLM
                     RGAALGYDALQDFTYLSQIAAVPNVVSVHPSVPAKNMNEFVVYLKSNPGKLNFGTPGN
                     GSLGHLTGETFKFAAGVDIVHVPYKGAAPALNDTLAGQVQVLFDNLPSSLPHIQAGRL
                     RALAVASEKRVASLPEIPTFAEAGLPLLNDPSWFGLIAPARLPADLAARVQAALGRAL
                     KRPETVQRLEAVAATPIGNTPAQFRAAVAAGIEASRRIAQQSNLKFD"
     gene            2749..>3323
                     /locus_tag="VGO02_03215"
     CDS             2749..>3323
                     /locus_tag="VGO02_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7476728.1"
                     /translation="MIRLSNVTLRRGVKVLLEGADVSLNPGEHAGLIGANGSGKSSLF
                     SVLRDELHADKGEVDFPRTWRVAHVAQETPALDRPAVEYAIDGDIALRTLQERLAGAE
                     KANDGNLIGELHAALGDADVYTVRSRAEALLVGLGFKHEELEQPVSSFSGGWRMRLNL
                     AQALMCRSELLLLDEPTNHLDLDAIVWLEDWL"
BASE COUNT          541 a         1092 c         1144 g          546 t
ORIGIN      
        1 atttcccgcg ccgctgtggc acgagctgga cggcgggcgc tacatcggaa cgggctgctc
       61 ggtggtgacg cgcgatttcg actccgagtg gatcaacgtc gggacctatc gggtgcaggt
      121 gatcgaccgc aaccacgtcg ccctcgacat ggtgcccggc aagcacggcc gcatccacta
      181 cgagaagtac aaggcggccg gcaagcgttt cccggtggtc atcgtctgcg gcgccgatcc
      241 gctcggctat ctgatctcgg ggatcgagat tccgttcggc atgtgcgaat ggaactatgt
      301 gggttccatt ttgaaagacc cggtatccgt tttgaaaggc gaagtgacgg gcctgccatt
      361 tccggccgcc tcggagatcg tgctggaagg cttcgtcgag ccgaacgacg agcgcaccga
      421 agggccgttc ggcgagttcc acggctacta ccccggcaag gccggtaccg cccccgtggt
      481 cacactggag cgaatctact tcagagattc cccaattatt gtcggcagcc cgccggccaa
      541 gccgccgaac gactactcgt attcaaaggc cgtgatgcgc tcggcgctgc tggccgacgc
      601 gctggccgcc gccggcgtgc ccgacgtgag ggcggtgtgg gcgcacgaga tcggcggtgc
      661 gcgcatgttc aacgtggtgt cgatcaagca gcgctacccc gggcacgcgc gccaggccgg
      721 ccacatcctc agccagtgcg gggtgggcgc ctacatgtcg aggtactcgg tggtggtcga
      781 cgacgacatc gacccgtcga acctgcagga agtgatgtgg gcggtggcga cgcgcaccga
      841 cccgtcggtg gacatcgaca tcatccagcg cggcgtcggc tcgaagaacg atccgatgtt
      901 cgtggcctac aagtacgacg cgccgctcag ctccaaggcc gtgatcgacg cctgccggcc
      961 ctgggagcac ctgggcgagt tcccaccggt cgccgaggcg agcaaggagc tgcaggacag
     1021 gacgcgctcg aagtggaagg aaattctcta ggcgccgccc gcgcatgtgc gggagcgcct
     1081 tttgctagta cattacggcc ccatgcacat cgaaacggtc aaggccgcct accgccgcta
     1141 cgccggcttc tacgacgcct tgttcgggcc ggtgctgcag cccggccggc gcgcggtcct
     1201 cgaggccctg cagctacgcc ccggggaccg ggtcctcgag gtgggcgtgg gcaccggcct
     1261 gtccctgccg atgtatccgc agaccgtcaa ggtgatcggg atcgacctgt cgagcgagat
     1321 gctggccaaa gcgcggctgc gcgtcgcgcg ccgcaagctt gccaacgtcg agtcgctgct
     1381 cgagatggac gccgagtcga tgagcttccc cgacgcgagc ttcgacaagg tggttgcgat
     1441 gtacgtgatg ccggtggtgc gcgacccggc gcgcgtgctc gaggagctgc accgcgtgtg
     1501 ccggcccgac ggcgagatct tcatcgtcaa ccacgtcaag tcggacaacc ggatcatccg
     1561 cgccttcgag atggggctgg cgcgcttttc caaccagatc ggcttccggc ccgatttcga
     1621 gctgcgcgag ctggtcaacg gcgccggcga gatcgacgaa gtggcgcgca tcaacgtctt
     1681 ctggcgcgtg atgcgcgtgc gcaacggcgt cgcccaccgc cgcctcgcgg cgcgctgacc
     1741 cctcagtcga acttcaggtt gctctgttgc gcgatgcgcc ggctcgcctc gatgccggcg
     1801 gccaccgctg cgcggaactg ggccggcgta ttgccgatcg gcgtcgccgc gacagcctcc
     1861 agccgctgca cggtctcggg ccgcttcagc gcgcggccga gcgcagcctg cacgcgcgcg
     1921 gccaggtccg ccggcaggcg cgccggcgcg atcaggccga accacgaggg atcgttgagc
     1981 agcggcaggc ccgcctcggc aaaggtcggg atctcgggca gcgaggcgac gcgcttctcc
     2041 gacgcgacgg cgagcgcccg cagccggccg gcctggatgt gcggcaggga cgagggcagg
     2101 ttgtcgaaca ggacctgcac ctggccggcg agcgtgtcgt tgagcgcggg cgccgccccc
     2161 ttgtagggca cgtgcacgat gtccaccccg gcggcgaact tgaaggtctc gccggtgagg
     2221 tggccgaggc tgccgttgcc cggtgtcccg aagttgagct tgccgggatt gcttttcaga
     2281 tagacaacaa actcgttcat gtttttcgcc ggcaccgacg ggtggaccga caccacgttc
     2341 ggcacggcgg cgatctggct caggtaggtg aagtcctgca gcgcgtcgta gcccagcgcg
     2401 gcgcctcgca tcaacgggac aactactggg ttgaccacgt gcgtcgagac ggtggcaaga
     2461 ccgagtgtgg agccgtcggg cgcggcctcc gcgatcgctt ttgcgccgat cgcgccgccg
     2521 gcgccgccgc ggttgtcgat gacgaagggc tggccgagct ccttcgccag gccctcggcg
     2581 gcgatgcgcc cgacgatgtc ggtcgagccg ccgggcggga aggggatgat caaggtaatc
     2641 gctcttgcgg gatagccttg cgcctgaatg gcgtttgaaa gaaccagcaa gcccgtcaag
     2701 aggtaaatcc gcatccgggc attctagctt cggtagaatc gcgcccccgt gatccgcctc
     2761 tccaacgtca ccctgcgccg cggcgtcaag gtcctcctcg agggcgccga cgtgtcgctc
     2821 aacccgggcg agcacgccgg cctgatcggc gccaacggct cgggcaagtc gagcctgttc
     2881 tcggtgctgc gcgacgagct gcacgccgat aagggcgaag tcgacttccc ccgcacctgg
     2941 cgcgtcgcgc atgtcgcgca ggaaaccccg gcgcttgacc ggccggcggt ggaatacgcg
     3001 attgacggtg atattgccct acggactctg caggaacgcc ttgccggtgc ggaaaaagca
     3061 aacgacggga acctgattgg agaactgcac gcggcgctcg gcgacgcgga tgtctacacg
     3121 gtgcgttccc gagccgaggc gctcctggtc ggccttggat tcaagcacga agagctggaa
     3181 cagccggttt ccagcttctc cggcggctgg cgcatgcgcc tgaacctggc gcaggcgctg
     3241 atgtgccgct ccgagctcct gctgctggat gagccgacca accacctgga cctcgatgcc
     3301 attgtctggc tggaagactg gct
//
LOCUS       DASYJA010000152         3411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_660645, whole genome shotgun sequence.
ACCESSION   DASYJA010000152 DASYJA010000000
VERSION     DASYJA010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660645"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VGO02_03220"
     CDS             complement(<1..226)
                     /locus_tag="VGO02_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476729.1"
                     /translation="MESIRIRGARTHNLKNLSLELPRNRLVVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLQLMEKPDVDL"
     gene            405..866
                     /gene="ssb"
                     /locus_tag="VGO02_03225"
     CDS             405..866
                     /gene="ssb"
                     /locus_tag="VGO02_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV7476730.1"
                     /translation="MASVNKVIVVGNLGADPETRFLPSGEAVTNIRVATTDKWKDKAS
                     GEMKEATEWHRISFFGRLAEVAGEYLKKGSQVYVEGSLRTRKWQDKDGQDRYSTEIRG
                     DVMQMLGRREGGGEPRAEVAERAEPKAAAAKPAAAKKPAGKFDDMEDDIPF"
     gene            887..1219
                     /locus_tag="VGO02_03230"
     CDS             887..1219
                     /locus_tag="VGO02_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV7476731.1"
                     /translation="MPIYEYRCDECGHQEDHLQKSSEKLLTKCPACGKKAYKKMLSAA
                     GFQLKGSGWYATDFKTAAKKPAEKKADLKSDAKGETKSDSKSESKTESKTESKPETKK
                     AATPAVTD"
     gene            1278..1883
                     /locus_tag="VGO02_03235"
     CDS             1278..1883
                     /locus_tag="VGO02_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEV7476732.1"
                     /translation="MKKYFITGLLIWIPLVATIWVLKFIFDVLDQSLLLLPIAFQTES
                     WLGVHIPGLGAILTLVIVLLTGVFATNFFGAQLVRLWHQVLNRIPVVNSIYSSVKQIS
                     DTLFSSSGQAFRKALLVQWPHPGMWTIAFLTGTPGGDVTRHIPEDCVSVYVPTTPNPT
                     GGYFVIVPRKDVVELDMTVDEALKYIISMGVAPPSPPAELR"
     gene            1883..>3411
                     /gene="aspS"
                     /locus_tag="VGO02_03240"
     CDS             1883..>3411
                     /gene="aspS"
                     /locus_tag="VGO02_03240"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEV7476733.1"
                     /translation="MRTHYCGEIDASLVERTVTLAGWAHRRRDHGGVIFVDLRDREGL
                     AQVVFNPERKDAFAIAERIRGEYVLRVTGKIRKRPQGSTNPELRSGEVELVAHEVEIL
                     NPSLTPPFQLDEDNLSETVRLENRVLDLRRAPMQRNLRLRHRAAMAARRFLDEHGFID
                     VETPFLYKSTPEGAREFLVPSRLHDAHFYALPQSPQLFKQMLMIAGFDRYYQIVKCFR
                     DEDLRADRQPEFTQIDVETSFLAEGEIRSLMEQLARHMFKEAAGIDLPDPFLVMRYDE
                     AMRDYGVDKPDLRVPLKLAELTDAMKDVDFKVFSGPASAPGGRVVALNVPGGGALTRG
                     EIDAYTEFVKALGAKGLAYIKVNEKAKGAEGLQSPIVKNLHAAAIKAILERSGAQDGD
                     LIFFGADREAACNAYMGALRQKVGHDRGLAQKGWRALWVVDFPMFEYDEETKEWRARH
                     HPFTSPKDGHEAYIGAAPEKAYDLVLNGWEIAGGSVRIHRQEVQQKVFEALKISPEQQ
                     RA"
BASE COUNT          662 a         1090 c         1123 g          536 t
ORIGIN      
        1 tcaggtcgac gtcgggcttc tccatcagct gcaggaactg gcgtgcgtag gccgagagcg
       61 actcgacgta gcggcgctgg ccctcggcgt acagcgtgtc gaaggcgagc gagctcttgc
      121 ccgagcccga cagcccggtg atcaccacca gccggttgcg cggcagctcg aggctgaggt
      181 tcttgaggtt gtgcgtccga gcaccgcgga tgcggatgct ttccattgat acgaaagccg
      241 aaaaggttaa cttggtacta taacgcgttt cgttttcccg ccgacagtcc tcctacggca
      301 ctactgtggt attcggcgca tgcgcctcac tggtcgtagc gggcagtcaa cgaggtttcg
      361 cgggcggcgt tgtgaggaga tcaattgatt gacgggggtg tgagatggca tcggtaaaca
      421 aagtaatcgt tgtaggcaac ctgggagcgg acccggagac gcggttcctg cccagcggcg
      481 aggcagtgac caacatccgc gtcgccacca ccgacaaatg gaaggacaag gcgagcggcg
      541 agatgaagga agccaccgag tggcaccgca tctcgttctt cgggcggctg gccgaggtcg
      601 ccggcgaata cctgaagaaa ggctcccagg tgtacgtcga aggcagcctg cgcacgcgca
      661 aatggcagga caaggacggc caggatcgct acagcaccga gatccgcggc gacgtcatgc
      721 agatgctcgg acggcgcgag ggcggcggcg agccgcgcgc cgaggtggcc gagcgcgctg
      781 agcccaaagc cgcggccgcg aagcccgctg cagcgaagaa gccggccggc aagttcgacg
      841 acatggaaga cgatattcct ttctgaaaca gggatataat cgcggcatgc cgatctacga
      901 ataccgctgc gacgagtgcg gccaccagga agaccatctg cagaagtcct ccgaaaagct
      961 gctgaccaaa tgtccggcct gcggcaagaa ggcctacaag aagatgctga gcgccgccgg
     1021 cttccagctg aaaggcagcg gctggtacgc caccgatttc aagaccgccg ccaagaagcc
     1081 ggcggagaag aaggcggatc tgaagtccga cgcgaagggc gagaccaagt cggactcgaa
     1141 gtctgaatcc aagaccgaat ccaagaccga atccaagccg gaaaccaaga aagcggccac
     1201 gccggccgtg accgactgat tcggagtact actgcgaacc ggcccggcgc gagccgggcc
     1261 ttttcatttc tggaacagtg aagaaatact tcatcaccgg cctgctgatc tggatcccgc
     1321 tggtcgcgac catctgggtg ctgaagttca tcttcgacgt cctcgaccag tcgctgctgc
     1381 tcctgccgat cgcgttccag accgagagct ggctcggcgt gcacatcccg ggactgggcg
     1441 cgatcctcac gctggtcatc gtgctgctca ccggtgtttt cgccaccaac ttcttcggcg
     1501 cgcagctggt gcgcctgtgg caccaggtgc tgaaccgcat cccggtggtg aactccatct
     1561 attccagcgt caagcagatc tcggacacgc tgttctcctc cagcggccag gcgtttcgca
     1621 aagcgctgct ggtgcagtgg ccgcacccgg gcatgtggac catcgccttt ctcaccggca
     1681 cccccggcgg cgacgtgacc cggcacatcc cggaggactg cgtcagcgtc tacgtgccga
     1741 ccaccccaaa cccgaccggc ggctacttcg tcatcgtgcc gcgcaaggac gtggtcgagc
     1801 tcgacatgac ggtggacgag gcgctgaagt acatcatctc catgggggta gcgccgccct
     1861 cgccgccggc ggagcttcgc tgatgcgcac gcactactgc ggcgagatcg acgcctcgct
     1921 ggtcgagcgc acggtgacgc tcgccggctg ggcgcaccgg cgccgcgacc acggcggcgt
     1981 catcttcgtt gacctgcgcg accgcgaggg gctcgcgcag gtggtcttca atcccgagcg
     2041 gaaggacgca ttcgccatcg cggagcgcat tcgcggcgaa tatgtgctca gagtcaccgg
     2101 caagatccgc aaacgcccgc agggcagcac caaccccgag ctgcgctccg gcgaagtgga
     2161 gctggtcgcg cacgaggtcg agatcctcaa cccttcgctg acgccgccgt tccagctcga
     2221 cgaggacaac ctgtcggaga cggtgcgact cgagaaccgc gttctcgatc tgcggcgcgc
     2281 gccgatgcag aggaacctgc gcctgcgcca ccgcgcggcg atggcggcgc gccgcttcct
     2341 cgacgagcac ggtttcatcg acgtggagac gcccttcctc tacaagtcga cgcccgaggg
     2401 cgcacgcgaa ttcctcgtgc cctcccggct gcacgacgct cacttctatg ccctgccgca
     2461 atcgccgcag ctcttcaagc agatgctgat gatcgcgggc ttcgatcgct actaccagat
     2521 cgtcaagtgc ttccgcgacg aggacctgcg cgccgaccgc cagcccgagt tcacgcagat
     2581 cgacgtcgag acctcgttcc ttgccgaggg tgaaatacgc tcgctgatgg agcagctcgc
     2641 gcgccacatg ttcaaggagg cggcgggcat cgatttgccc gatccgttcc tcgtgatgcg
     2701 ctacgacgag gcgatgcgcg actacggcgt cgacaagccg gacctgcgcg tgccgctcaa
     2761 gctcgccgag ctgaccgacg cgatgaagga cgtcgacttc aaggttttct ccggaccggc
     2821 gagcgcgccg ggcggccggg tggtggcgct gaacgtgccg ggcggaggcg cactgacgcg
     2881 cggcgagatc gatgcctaca ccgagttcgt caaggcgctc ggcgccaagg ggctcgccta
     2941 catcaaggtg aacgagaagg cgaagggcgc cgaaggcctg cagtcgccga tcgtgaagaa
     3001 cctgcatgca gctgcgatca aggcgattct cgagcgctcc ggcgcccagg acggcgacct
     3061 gatcttcttc ggcgcggacc gggaagccgc gtgcaacgcc tatatggggg cgctgcggca
     3121 gaaagtgggc catgaccgcg gactcgcgca gaagggctgg cgagcgctgt gggtggtcga
     3181 cttcccgatg ttcgagtacg acgaggaaac gaaggagtgg cgggcgcgcc accacccgtt
     3241 caccagcccg aaggacgggc acgaggcgta catcggagcc gcgccggaga aggcctacga
     3301 ccttgtgctg aacggctggg agatcgccgg cggctcggtg cgcatccacc gccaggaagt
     3361 gcagcagaag gtgttcgagg cgctcaagat cagccccgag cagcagcgcg c
//
LOCUS       DASYJA010000153         4276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3203032, whole genome shotgun sequence.
ACCESSION   DASYJA010000153 DASYJA010000000
VERSION     DASYJA010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3203032"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VGO02_03245"
     CDS             complement(<1..583)
                     /locus_tag="VGO02_03245"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7476734.1"
                     /translation="MRRLLLAGAGHAHLALLASLAKEPVRGLRITLVSPRERQIYSGM
                     LPGIIAGHYRAEEAEIDVARLAERAYVEFRAGAVARFDAQQRAVVLHDGTELAYDLAS
                     FSVGSRIDAAIPGAAYATAVKPFEPLLQRLAQGAFKRIAIVGGGVGGAEIAMALRFHG
                     AAVTLYSEHPMMPPALVARVERVLRGMGVDFRPG"
     gene            complement(591..2219)
                     /locus_tag="VGO02_03250"
     CDS             complement(591..2219)
                     /locus_tag="VGO02_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7476735.1"
                     /translation="MVNRYEELYRSFRWDVPARYNIAHACCGQWAGDGTRVALHWEDE
                     SGAAATYTFRDIQVAANRLSNALSALGVKRGDRVALLLPQRPETAIAYMAIFQMGAIA
                     LPLSHLFGPDALEYRLEHAGASVAIAEPTTLPNLLAIKDRLSQLKHVIGVDVAGVLDW
                     KKLLDKGSSDFHPVDTSAEDPALIVYTSGTTGPPKGALKAHRVMLGNVPGFVHSHDFF
                     PQKGDMFWSPADWAWTGGLMDALLPAWLFGVPILGYRGRFDAEKAYYLLEKYAIRNSF
                     LFPTALKLMMKAVPEPRAKYRLNLRSIMSAGESVGVTVIEWAREQLGVTINEMFGQTE
                     INYVVGNCHAAWPVKPGSIGRPYPGHKVAVIDDQGNEVPRGELGEIAVNRSCNGTRDP
                     VFFLEYWKNEKATRDKFIGEWGCTGDQGRMDEDGYLWYQGRSDDVIKSSGYRIGPAEI
                     ESCLVKHPAVANAAVIGKPDATRGAIVKAFIVLQPGNVPSDKLTEEIQNHVRGRLAPY
                     EYPREIEFIDALPMTTTGKVQRKELRKREESKLK"
     gene            2247..2888
                     /locus_tag="VGO02_03255"
     CDS             2247..2888
                     /locus_tag="VGO02_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEV7476736.1"
                     /translation="MAIDVLLYDAYGTLWDVHSVMQRCESCWPGKGRQLSQLWRAKQL
                     EATWQRSLMGRYVPFSTVTREALEYACEALGLALQKDHESELMGEYLKLATFPDVADA
                     LMRKVAAQTGILSNGSPDMLEPLVLHSGLKFDAVLSVDTAKVFKPAPAAYRIALERFG
                     VPKERIGFVSSNFWDAAGAKSFGFTVYWINRGGVAAERLGFQPDRVLKTLAAL"
     gene            2905..3435
                     /locus_tag="VGO02_03260"
     CDS             2905..3435
                     /locus_tag="VGO02_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV7476737.1"
                     /translation="MKFCCYCASPVVSRVPPGDSLPRWICDECGEIHYQNPKLVLGTI
                     PEYQGRILLCRRAIEPRYGYWTLPAGFMENSETTGQAAARETLEEAGARVDLGAPFSM
                     ISVPRVNQVHLFYQARLLDLDFKPGEETLEVAMFDEAGVPWNDIAFRTVGLTLKLWLS
                     DRKAGTFTFHAEDINS"
     gene            3506..3976
                     /locus_tag="VGO02_03265"
     CDS             3506..3976
                     /locus_tag="VGO02_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEV7476738.1"
                     /translation="MGARGLLLLTLVLAASGACGGTLRGQVVGISDGDTLTLLVDRKQ
                     IKVRLTEIDAPEMKQAFGSRSRQSLGELCGRHAATVRTSGKDRYGRVLGRVECGGVDA
                     NAEQVRRGMAWVYDRYVTDRSLYALQDEARAAHAGLWADAAPTAPWAWRAKNKR"
     gene            4027..>4276
                     /locus_tag="VGO02_03270"
     CDS             4027..>4276
                     /locus_tag="VGO02_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HEV7476739.1"
                     /translation="MKIDPANTAWMLTASVLVLFMTLPGLALFYAGLTRTKNVLSVLM
                     QCFAITCVVTLAWVVVGYSIAFGDGGAANAWWGGLGKSM"
BASE COUNT          705 a         1397 c         1435 g          739 t
ORIGIN      
        1 tgccgggacg gaagtcgacg cccatgccgc gcagcacgcg ctcgacgcgc gccacgagcg
       61 ccggcggcat catgggatgc tccgagtaca gcgtgaccgc ggcgccgtga aagcgcagcg
      121 ccatggcgat ctcggcgccg ccgacgccgc cgccgacgat ggcgatgcgc ttgaatgcgc
      181 cctgcgccag gcgctggaga agcggctcga aaggcttcac cgcggtggcg taggccgcgc
      241 ccgggatcgc cgcgtcgatg cgcgagccga cgctgaagct ggcgaggtca tacgcgagct
      301 cggtgccgtc gtgcaggacc accgcgcgct gctgcgcatc gaagcgcgcc accgcgccgg
      361 cgcggaactc gacataggcg cgctccgcca ggcgggcgac gtcgatctcg gcctcctcgg
      421 cgcggtagtg cccggcgatg atcccgggca gcatgcccga atagatctgc cgctcgcgcg
      481 gcgagaccag cgtgatgcgc aggccgcgca ccggctcttt ggcaagggac gcgagcagcg
      541 cgaggtgcgc gtgcccggcg ccggcgagga gaaggcgcct cacgaggcag ctacttcagc
      601 ttcgactctt cgcgcttgcg cagctccttg cgctgcacct tgccggtggt ggtcatcggc
      661 agcgcatcga tgaactcgat ctcgcgcggg tactcgtacg gagccagcct gccacgcacg
      721 tgattctgta tctcttcagt caatttatcc gacggcacat ttccaggttg aagaacaata
      781 aaggccttca cgatcgcgcc gcgggtcgca tcgggcttgc cgatcactgc cgcgttcgcc
      841 accgccgggt gcttcaccag gcagctttcg atctcggccg gaccgatgcg gtagcccgag
      901 cttttgatga cgtcgtcgga gcgtccctgg taccagaggt agccgtcctc gtccatgcgg
      961 ccctggtcgc cggtgcagcc ccattcgccg atgaatttgt cgcgcgtcgc cttctcgttc
     1021 ttccagtatt cgaggaaaaa aaccggatcc cgcgtgccgt tgcatgatct gttcaccgct
     1081 atctctccca gttcccccct gggtacttcg ttgccctggt cgtcgatcac cgcgaccttg
     1141 tggcccggat acggccggcc gatcgagccg ggcttcaccg gccaggcggc atggcagttg
     1201 cccactacgt agttgatctc ggtctggccg aacatctcgt tgatggtgac gccgagctgc
     1261 tcgcgcgccc actcgatgac cgtgacgccg accgattcgc cggcgctcat gatcgatcgc
     1321 agattcaacc ggtattttgc cctgggctcg ggcaccgcct tcatcatgag ctttagggcc
     1381 gtggggaaca ggaaagagtt ccggatggcg tacttctcca gcaggtagta ggccttctcg
     1441 gcgtcgaagc gcccgcggta gccgaggatc gggacgccga acagccacgc cggcaatagc
     1501 gcgtccatca ggccgccggt ccacgcccag tcggccggcg accagaacat gtcgcccttc
     1561 tgcgggaaga agtcgtgcga atgcacgaag cccggcacgt tgccgagcat cacgcggtgc
     1621 gccttcagcg cgcccttcgg cggcccggtg gtgccgctgg tgtagacgat caatgccggg
     1681 tcctcggcgg aggtatcgac cggatggaaa tcgctgctcc ccttgtcgag gagttttttc
     1741 caatcgagaa ccccggcaac atcgaccccg atcacgtgct tcagctgcga gagccggtcc
     1801 ttgatagcca gaaggttggg cagcgtggtc ggctcggcga tggcgacgct tgccccggcg
     1861 tgctccaggc ggtactcgag cgcgtccggg ccgaacaggt gcgaaagggg cagcgcgatc
     1921 gcgcccatct ggaagatcgc catgtaggcg atcgcggtct ccgggcgctg cggcagcagg
     1981 agcgccacgc gatcgccgcg cttgaccccg agcgccgaga gcgcgttcga caggcggttc
     2041 gcggcgacct ggatgtcgcg gaacgtgtac gtggcggcgg cccccgactc gtcttcccaa
     2101 tggagagcga cgcgcgtgcc atcgcccgcc cattgcccgc agcaggcgtg cgcgatgttg
     2161 tagcgcgcgg gaacgtccca ccggaagcta cggtagagct cttcgtagcg gttgaccatg
     2221 cgccggggca gtttaacatc ggggaaatgg cgatcgacgt cctcctctac gacgcgtacg
     2281 gcacgctgtg ggacgtgcac tcggtgatgc agcgctgcga gagctgctgg ccgggcaagg
     2341 gcaggcagct gtcgcagctg tggcgcgcga agcagctcga ggcgacctgg cagcgcagcc
     2401 tgatggggcg ctacgtgccg ttctcgaccg tcacgcgaga agcgctcgaa tacgcgtgtg
     2461 aagcgctcgg ccttgcgctg cagaaggatc acgagagcga gctgatgggc gagtatctga
     2521 agctcgcgac gttccccgac gtcgccgatg cactgatgcg caaggtcgca gcgcagaccg
     2581 gcatcctgtc gaacggctca cccgacatgc tggagccgct ggtgctgcac tcggggctga
     2641 agttcgacgc cgtcctcagc gtcgacacgg cgaaggtgtt caagccggcg cccgcggcct
     2701 accggatcgc gctcgagcgc ttcggcgtgc cgaaggagag gatcggattc gtctcctcca
     2761 atttctggga cgcggccggc gccaagtcct tcggcttcac ggtctactgg atcaatcgcg
     2821 gcggcgtggc ggcggagcgc ctcggcttcc agcccgatcg cgtcctgaag acgctggccg
     2881 cgctatgacg tcctgctgcg tgttatgaag ttctgttgtt actgtgcctc gccggtggtg
     2941 agccgcgtgc ccccaggcga cagcctgccg cgctggatct gcgacgagtg cggcgagatc
     3001 cattaccaga accccaagct ggtgctcggc accattcccg agtaccaggg caggatcctg
     3061 ctgtgccggc gcgcgatcga gccgcgctac ggctactgga cgctgcccgc cggcttcatg
     3121 gagaacagcg agaccaccgg ccaggcggcg gcgcgcgaaa cgctcgagga agccggcgcg
     3181 cgtgtcgacc tcggcgcgcc gttcagcatg atctcggtgc cgcgcgtgaa ccaggtgcat
     3241 ctgttttacc aggcgcggct tctcgacctc gacttcaagc cgggcgagga aaccctcgaa
     3301 gtagcgatgt tcgacgaggc cggggtgccc tggaacgaca tcgccttccg gaccgttgga
     3361 ctcacgctca aactctggtt gtccgaccgc aaggccggca cgttcacttt tcacgctgaa
     3421 gacattaatt cataaagact ttttgcagtc cgcgtgaaga agtgctttta tcagaaagtc
     3481 ttagaccggg tagtattcgc gcgccatggg agcgcgcggg ctactactac tcacccttgt
     3541 cctggcagcc tccggggcct gcggggggac tctgcggggg caagtcgtcg gcatcagtga
     3601 cggcgacacg ctcacgctcc tcgtcgaccg caagcagatc aaggtgcggc tgaccgagat
     3661 cgacgctccg gagatgaagc aggcgttcgg cagccgctcg aggcaatccc tgggcgagct
     3721 gtgcggccgg cacgccgcga cggtccggac ttccggcaag gaccgctacg ggcgggtgct
     3781 ggggcgcgtg gagtgcggcg gcgtcgacgc gaacgccgag caagtgcgcc gcggcatggc
     3841 ctgggtgtac gaccgctacg tcaccgaccg cagcctttac gcactgcaag acgaggcgcg
     3901 ggcggcgcat gccggcctgt gggcggatgc cgcgccgacg gcgccctggg cctggcgcgc
     3961 gaaaaataaa cgttaaggta tgcttcctcc cggtgcgccc catgcaccga aaacgggagg
     4021 ataaaaatga agatcgaccc cgccaacacg gcgtggatgc tcaccgcatc cgttcttgtg
     4081 ctgttcatga cgctccccgg cctggctttg ttctacgccg gtctgacacg cacgaaaaac
     4141 gtgctctctg tcctaatgca gtgcttcgcg atcacctgcg tggtcacgct cgcgtgggtg
     4201 gtggtcggct acagcatcgc cttcggcgac ggcggtgccg ccaacgcctg gtggggcggc
     4261 ctgggtaaat cgatgc
//
LOCUS       DASYJA010000154         2273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_24825, whole genome shotgun sequence.
ACCESSION   DASYJA010000154 DASYJA010000000
VERSION     DASYJA010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24825"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VGO02_03275"
     CDS             complement(<1..245)
                     /locus_tag="VGO02_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7476740.1"
                     /translation="MALPPRKAVVAADTDVLVVGGGPAGLGAAVGAADAGARVVLAER
                     YGFLGGNATAALVMPLMSFHTQHHQPERRGVTTLLPT"
     gene            complement(259..1410)
                     /locus_tag="VGO02_03280"
     CDS             complement(259..1410)
                     /locus_tag="VGO02_03280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEV7476741.1"
                     /translation="MANGKETYRIGITGSYGGLNLGDEAILQSIVAQLRRDLPAEITV
                     FSRDAEDTKRRHGVERAVPVRKLSRSEVTPEIERLDLLILGGGGILYDADARTYLREV
                     SIAREKGVPVMLYAVGAGPLVHAGAQAAVREELEHVEAITVRERSAQRVLEEAGVHRE
                     IIVTADPALLLKPEPLPRGLLKREGLEGRRRLIGMSVREPGVAAPDLDQKVYHALLAN
                     AADFMVDRYDADVMFVPMERSVLDTQHSHAVVASMLRAQRATVLKGKYSSGQLLTLMS
                     RFDFVVGMRLHFLIFAALAGTPFVALPYAGKVAGFLEDLGLPAPPLNLVNPGRLIAHI
                     DESWDRRRATRAKLEKAVPPLQERAQQTNRIVVELLKKGKLRLASPQAA"
     gene            1453..1827
                     /locus_tag="VGO02_03285"
     CDS             1453..1827
                     /locus_tag="VGO02_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7476742.1"
                     /translation="MGSGKRILVVDDNEDLRSTIKALLQADGFDVSVAADGEAALASH
                     RAHPADVVVTDLFMPDKDGIETIIELKKLYPTVKIVAMSGWTSTQGSDYLQVAREIGA
                     AVTLQKPFDPAELSRVVRDLTT"
     gene            complement(1796..>2273)
                     /locus_tag="VGO02_03290"
     CDS             complement(1796..>2273)
                     /locus_tag="VGO02_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7476743.1"
                     /translation="LTAVANRSEKIPSIQGRKTVMAGRRVLVVDDNVDAAETVAAYLR
                     LEGHEAKVVTEGAEVLGSARVFEPQVVVLDIGLPGVDGYQIARQLRSEPQTRDTLLIA
                     VTGYGRDEDRALAAAAGFDCHFVKPADPVEIQAVIERGRQAGAGVALRSSGRVPPG"
BASE COUNT          335 a          809 c          778 g          351 t
ORIGIN      
        1 tcggtcggca gcagcgtggt gacgccgcgc cgctcgggct ggtggtgctg cgtgtggaag
       61 gacatgagcg gcatcaccag cgccgcggtg gcgttgccgc cgagaaagcc gtagcgctcg
      121 gcgagcacga cgcgcgcgcc ggcgtcggcc gcgcccactg ccgcgccgag ccccgccggc
      181 ccgccgccga cgaccagcac gtcggtgtcc gccgcgacca cggccttgcg cggcggcagc
      241 gcgatgcgcg ccgcgcgctc aggccgcttg agggctggca aggcgcagct ttcctttctt
      301 cagcagctct acgacgatgc gattggtctg ctgcgcgcgc tcctgcagcg gcggcaccgc
      361 cttctccagc ttcgcccggg tggcgcgccg ccggtcccag gattcgtcga tgtgcgcgat
      421 cagccggccc gggttcacca ggttgagcgg cggcgccggc aggccgagat cctcgagaaa
      481 gccggccacc ttgccggcgt agggcagcgc cacgaacggc gtgccggcga gcgcggcgaa
      541 gatcaggaag tgcagccgca tgccgaccac gaagtcgaag cgcgacatga gcgtgagcag
      601 ctgaccggag ctgtacttgc ccttgagcac ggtcgcgcgc tgcgcgcgca gcatgctggc
      661 gacgactgcg tggctgtgtt gcgtgtcgag caccgagcgc tccatcggca cgaacatcac
      721 gtcggcgtcg tagcggtcga ccatgaagtc ggcggcgttg gcgagcagcg cgtgatacac
      781 cttctggtcg aggtcgggcg cggcgacgcc gggctcgcgc accgacatgc cgatcagccg
      841 ccgcctgccc tccagcccct cgcgcttgag caggccacgc ggcagcggct cgggcttgag
      901 cagcagcgcc gggtcggcgg tgacgatgat ctcgcggtgc actccggctt cctccagcac
      961 gcgctgcgcg gagcgctcgc gcaccgtgat cgcctcgacg tgctcgagct cttcgcgcac
     1021 tgccgcctgc gcgcccgcgt ggaccagcgg tccggcgccg acggcgtaga gcatcaccgg
     1081 cacgcctttc tcgcgcgcga tgctcacctc gcgcaggtag gtgcgcgcat cggcgtcgta
     1141 caggatgccg ccgccgccga ggatcagcag gtcgagccgc tcgatctcgg gcgtcacttc
     1201 cgagcgcgac agcttgcgca ccggcaccgc gcgctcgacg ccgtggcgtc gcttggtgtc
     1261 ctcggcgtcg cgcgagaaca cggtgatttc cgccggcaga tcgcggcgca gctgggccac
     1321 gatggactgc aggatggcct cgtcgcccag gttgagcccg ccgtacgagc cggtaattcc
     1381 gatcctgtag gtttcttttc cgtttgccat ccgcgaacac tatgcaagaa cgattccact
     1441 tagaatgtcg ccatggggtc cggcaagcgc atcctggtgg tcgacgacaa tgaggacctc
     1501 aggtccacca tcaaggcgtt gctgcaggcc gacggtttcg acgtgtcggt ggcggccgac
     1561 ggcgaagccg cccttgcctc gcaccgcgcc catccggccg acgtagtggt caccgacctc
     1621 ttcatgcccg acaaggacgg catcgagacc atcatcgagc tgaagaagct ctacccgacg
     1681 gtcaagatcg tggccatgtc gggctggacc tcgacgcagg gctccgacta cctccaggtg
     1741 gcgcgcgaaa tcggcgccgc ggtcacgctg cagaagccct tcgaccccgc cgagctcagc
     1801 cgggtggtac gcgacctgac gacctgagcg cgaccccggc gccggcctgg cgaccgcgct
     1861 cgatgacggc ctggatctcg accgggtcgg ccggcttcac gaagtggcag tcgaaccccg
     1921 cggcggcggc gagcgcccgg tcctcgtcgc gtccatagcc ggttaccgcg atgagcagcg
     1981 tgtcgcgcgt ctgcggctcg gagcgcagct gacgcgcgat ctggtagcca tccacccccg
     2041 gcaggccgat gtcgagcacc accacctgcg gctcgaacac gcgcgccgag ccgagcacct
     2101 cggcgccctc ggtgaccacc ttggcctcgt gcccttccag cctgagatag gcggcgacgg
     2161 tctcggcggc gtcgacgttg tcatcgacca ccagtacccg ccggcctgcc atgaccgttt
     2221 tcctgccttg aattgaagga atcttctcgg agcggtttgc aacggctgtc agg
//
LOCUS       DASYJA010000155         1976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_843288, whole genome shotgun sequence.
ACCESSION   DASYJA010000155 DASYJA010000000
VERSION     DASYJA010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843288"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VGO02_03295"
     CDS             <1..629
                     /locus_tag="VGO02_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV7476744.1"
                     /translation="AVAAVSQAQRAIGMPTSVTGSYSGDSAEFDKSLAGQPWLILAAA
                     ITIYIVLGVLYESYVHPLTILSTLPSAGVGAVLALMVCNTEFSIIALIGVILLIGIVK
                     KNAIMMIDFALVAERSRNLNAEQSIYEACLLRFRPIMMTTMAALLGALPLVLRHGDGA
                     ELRQPLGITIVGGLIFSQLLTLYTTPVIYLYLDRFRLWARQRLRLKAA"
     gene            626..>1976
                     /locus_tag="VGO02_03300"
     CDS             626..>1976
                     /locus_tag="VGO02_03300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEV7476745.1"
                     /translation="MKKALLITALLAGCAVGPDYKRPEADIPMQFKEMQGWRVATPAD
                     AAPRGSWWAVFGDAELDALMARVDVSNQTIRGAEARFRQARALSDQARSGLYPSVSAN
                     GTITRSKQPSLPNAPSVTGAVTNYNASFNASWEPDLWGSVRRAVEAGDASWQASAAEL
                     EAARLSARATLAQSYFALRVADSNRQLLEDTVAAYARTLEMTRNRYTAGVVARVDVVQ
                     AEVQWKSAQAQLIDLAVERGQLEHAIALLVGEAPANFAIERKPLALAMPQIPVGLPSE
                     LLERRPDVASAERNAAAANARIGVAKAAYFPAITLNGAVGYRTTNVADIFALPSRFWS
                     IGGSIAQAVFDAGLRKAQTAQAIAAYDEQVANYRQTVLTGFQEVEDNLVALRVLEEEA
                     RLQDEVVQAARQAVELTTNQYRAGVVSYLNVIQAQATQLANERTAASILGRRLTASVG
                     "
BASE COUNT          311 a          716 c          661 g          288 t
ORIGIN      
        1 acgcggtcgc cgcggtgtcc caggcgcagc gcgcgatcgg gatgccgacc tcggtgaccg
       61 ggtcctattc cggcgactcc gccgagttcg acaagtcgct ggccggccaa ccgtggctca
      121 tcctcgccgc cgcgatcacg atctacatcg tgctgggcgt gctgtatgag agctatgtcc
      181 atccgctgac catcctgtcc acgctgccct ccgccggggt cggcgcggtg ctggcgctga
      241 tggtgtgcaa caccgagttc agcatcatcg cgctgatcgg cgtcatcctg ctcatcggca
      301 tcgtcaagaa gaacgcgatc atgatgatcg acttcgcgct ggtcgccgag cgcagccgca
      361 atctcaacgc ggaacaatcg atctacgagg cctgcctgct gcgcttccgc ccgatcatga
      421 tgaccacgat ggcggcgctg ctcggcgcgc tgccgctcgt gctgcgccac ggcgacggcg
      481 ccgagctgcg ccagccgctc ggcatcacca tcgtcggcgg gctgatcttc agccagcttc
      541 tcacgctcta cacgacgccg gtgatctacc tctacctcga ccggttccgg ctgtgggcga
      601 ggcaaagatt gcggttgaaa gcagcatgaa aaaagccctt ctcatcacag ctcttctcgc
      661 cggatgcgcg gtgggcccgg actacaagcg tccggaggcc gacatcccaa tgcaattcaa
      721 ggaaatgcag ggctggcgcg tggcgacgcc ggccgacgcc gcgccgcgcg gctcctggtg
      781 ggcggtgttc ggcgatgccg agctcgacgc gctgatggcg cgggtcgacg tttccaacca
      841 aacgatccgc ggcgcggagg cgcgcttccg ccaggcgcgc gcgctctccg accaggcgcg
      901 ctccggcctg tacccgtcgg tgagcgcgaa cggcaccatc acccgcagca agcagccgtc
      961 gcttcccaac gcgccgtccg tgaccggcgc ggtcaccaac tacaacgctt cattcaacgc
     1021 gagctgggag cccgacctgt ggggcagcgt gcgccgcgcg gtcgaggcgg gcgacgcgag
     1081 ctggcaagcg agcgcagcgg agctcgaggc ggcgcgcctc tctgcccgcg cgaccctcgc
     1141 gcagagctac tttgcgctgc gcgtggccga ctccaaccgc cagctgctgg aagacacggt
     1201 ggcggcctac gcccgcacgc tcgagatgac gcgcaaccgc tacaccgccg gcgtggtggc
     1261 gcgcgtcgac gtggtgcagg ccgaggtgca atggaagagc gcgcaggccc agctcatcga
     1321 cctggcggtc gagcgcggcc agctcgagca cgccatcgcg ctgctggtgg gtgaagcgcc
     1381 ggcgaacttc gccatcgagc gcaagccgct cgcgctcgcc atgccgcaga tccccgtcgg
     1441 gctgccctcc gagctgctcg agcgccggcc cgacgtcgcc tccgccgagc gcaacgccgc
     1501 cgcggcgaac gcgcgcatcg gcgtggcgaa ggccgcctac ttcccggcca tcacgctgaa
     1561 cggcgccgtg gggtatcgca ccaccaacgt cgccgacatc ttcgcgctgc cgagccgctt
     1621 ctggtcgatc ggcggctcga tcgcgcaggc ggtgttcgac gccggcctgc gcaaggcgca
     1681 gaccgcgcag gcgatcgccg cctacgacga gcaggtcgcc aactaccgcc agaccgtgct
     1741 caccggcttc caggaggtcg aggacaacct ggtcgcgctg cgcgtgctcg aggaggaggc
     1801 gcgcctgcag gacgaggtgg tgcaggcggc gcgccaggct gtcgagctca ccaccaacca
     1861 gtaccgcgcc ggcgtggtga gctacctcaa cgtcatccag gcgcaggcga cgcagctcgc
     1921 caacgagcgc accgcggcga gcatcctcgg gcgtcgcctc accgccagcg tgggat
//
LOCUS       DASYJA010000156         3998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1931844, whole genome shotgun sequence.
ACCESSION   DASYJA010000156 DASYJA010000000
VERSION     DASYJA010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1931844"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1587
                     /locus_tag="VGO02_03305"
     CDS             <1..1587
                     /locus_tag="VGO02_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HEV7476746.1"
                     /translation="ELLVLASLIAFLTMEGTRRTSGYSLIVITFVLVVWVLLGHLVPG
                     QLRTHKVEAGNLAVYLNFDNNGLLGLVLEIACTIVIAFVLMGQLLARSGGSGFFNDFA
                     LGLMGRFRGGAAKIAVVASSLFGSISGIAAASALAVGVVTIPLMKKSGIPARLAAAIE
                     ACASNGAQLMPPVMGAVAFVMADFLQVPYREVALAALLPSLLYYSALFIQCDLETARH
                     GFGKVAPSEIPRMGPVLATGWIFLAPFVAIIGAMFWLNWEPEPAAALAAAIIIGLGLF
                     IGYRGARMKLADIWSAVIETGIGVCEIIVISAIGGYVLGLFQVGGLSFALTAYLVNLG
                     AENLLLLLVICAVTNIVLGLGLPTLAVYVMLAILVAPALIKVGVQPMAAHLFILYFGI
                     MSLITPPIATAAFVAATIAKTDPMAAGWTAMRFGWTSYIVPFLFVYSPALIMRSGPLE
                     TVAVMALSLAGIWFVCAAFTGYAFRVMGTAMRVGFGAAGFLLLLPFQAATAFGWLNAA
                     GAALGLTLLFLEKRGAYVRA"
     gene            1574..2608
                     /locus_tag="VGO02_03310"
     CDS             1574..2608
                     /locus_tag="VGO02_03310"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEV7476747.1"
                     /translation="MYVRNCWYVAAWDHEVTRTMKRRVLLDEPVVLFRKDDGTPVALE
                     DRCCHRQAPLSMGKLVGNIVECPYHGLQFDVAGKCVKVPSQDLVPSSAKVRSYPVFEK
                     NQWIWIWMGDPAKADPSLIEDFHWLDDPKWGAAGSYLHVEANYQLLVENLLDTTHLPF
                     LHPTSLGTDAFARSEFEVTREADRVKVTRWLMNELPAPFHKKMGGFPDGMKVDRWQIA
                     AFSPPCFVKLDVGSAPAGTGARQGDRKGHMNMWNLNAITPETEKTAHYFFAQAYNVRV
                     NEKWMQEVVRAQVVGAFLEDMAMIKAQQKNMDLGASAVVNLGQDKAWIVMRQIVERLV
                     KEEQQQVRAA"
     gene            2605..2892
                     /locus_tag="VGO02_03315"
     CDS             2605..2892
                     /locus_tag="VGO02_03315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7476748.1"
                     /translation="MSDVVRREDPAHKGKGAWVIERDGEILAELGFTTAGSRVILDHT
                     DVSDALRGTGSGKKLVEAAVLWARGANVKLIPLCPFAKSVFDKTPEIRDVL"
     gene            complement(2896..>3998)
                     /locus_tag="VGO02_03320"
     CDS             complement(2896..>3998)
                     /locus_tag="VGO02_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEV7476749.1"
                     /translation="SLPRPHDLLDLMKAKASGKPYDKTAYEGRVKTAVAECVRKQLEA
                     GIDIVTDGEQSKPGFFLYVNERLEGFTPRPGMKRKFFDAEVAAFPDYYAQYFKQAMIG
                     GAVAPVVPMVCTAPVKYRGEAAIARDIANLKAAAAGAKGVFMPAVAPSGVGTNEYYKT
                     EEEYFHAVGAALRAEYKAIVDAGFTLQIDDPFLSELFADQEYDMKGRRARAAMYVEAL
                     NESLRGIPQEKVRFHTCYGINEGPRIHEAALADVVDAMLAVNAGAYSFEAANPRHEHE
                     YHLWETVKLADGKKLIPGVITHASNIVEHPELIAERLLRYTNLVGADSVIAGADCGFS
                     SQATYTTEVHPSVVWAKFQAMREGARLASAKL"
BASE COUNT          604 a         1351 c         1302 g          741 t
ORIGIN      
        1 gaattattgg ttttggcaag ccttattgcc tttctcacca tggaaggaac gcgccgcact
       61 tcgggctact cgctcatcgt catcaccttc gtgctggtcg tctgggtgct gctcggccac
      121 ctcgtcccgg ggcagctgcg cacgcacaag gtcgaggcgg gaaacctcgc cgtctacctc
      181 aacttcgaca acaacggcct gctcggcctc gtgctcgaga tcgcctgcac catcgtcatc
      241 gccttcgtgc tgatgggcca gctcctcgcg cgctcgggcg gctcgggctt cttcaacgac
      301 tttgccctcg ggctgatggg ccgcttccgc ggcggcgcag ccaagatcgc ggtcgtcgct
      361 tcgagcctgt tcggcagcat ctcgggcatc gcggcggcga gcgccctcgc cgtcggcgtg
      421 gtgacgattc cgctgatgaa gaagtccggc atcccggcgc ggctcgcggc ggcgatcgag
      481 gcctgcgctt ccaacggcgc gcagctcatg ccgccggtga tgggcgcagt ggctttcgtc
      541 atggccgact tcctgcaggt gccctaccgc gaggtggcgc tcgccgccct gctgccttcg
      601 ctgctctact actcggcgct gttcatccag tgcgacctcg agaccgcgcg ccacggcttc
      661 ggcaaggtgg cgccgagcga gattccgcgc atgggaccgg tgctggcgac gggctggatc
      721 ttcctcgcgc ccttcgtcgc catcatcggc gccatgttct ggctgaactg ggagcccgag
      781 ccggcggcgg cgctcgccgc ggcgatcata atcggcctgg gcctgttcat cggctaccgc
      841 ggcgcgcgca tgaagctcgc ggacatctgg agcgcggtga tcgagaccgg catcggcgtg
      901 tgcgagatca tcgtcatcag cgcgatcggc ggctacgtcc tcggcctgtt ccaggtcggc
      961 gggctgtcgt tcgcgctcac cgcctacctc gtcaacctcg gcgccgagaa cctgctcctg
     1021 ctgctggtca tctgcgccgt gaccaacatc gtgctcggcc tcggtctgcc gacgctcgcg
     1081 gtctatgtga tgctcgcaat cctggtggcg ccggcgctga tcaaggtcgg cgtgcagccg
     1141 atggcggcgc acctcttcat cctgtacttc ggcatcatgt cgctcatcac gccgccgatc
     1201 gccaccgccg ccttcgtcgc cgccaccatc gccaagaccg acccgatggc cgccggctgg
     1261 accgcgatgc gcttcggctg gacctcctac atcgtgccgt tcctcttcgt ctactcgccg
     1321 gcgctgatca tgcgctccgg ccccctggaa accgtcgctg tgatggcgct gtcgctcgcc
     1381 ggcatctggt tcgtctgcgc cgccttcacc ggctatgcct tccgggtgat gggcacggcc
     1441 atgcgggtag gtttcggcgc ggccggtttt ctgctattgc tacccttcca ggccgcgacg
     1501 gccttcggct ggctgaacgc tgccggtgct gctctcgggc ttacattatt gtttcttgaa
     1561 aaaagaggcg cctatgtacg tgcgtaactg ctggtacgtc gcagcatggg accacgaggt
     1621 cacgcgcacg atgaagcggc gcgtcctgct cgacgagccg gtggtgctgt tccgcaagga
     1681 cgacggcacg ccggtcgcgc tggaagaccg ctgctgccac cggcaggcgc cgctgtcgat
     1741 gggcaagctg gtcggcaaca tcgtcgagtg cccctatcac ggcctgcagt tcgacgtggc
     1801 aggaaagtgc gtcaaggtgc ccagccagga cctggttccc tcgtctgcga aggtgcggag
     1861 ctatccggtt ttcgagaaga accagtggat ctggatctgg atgggcgacc cggccaaggc
     1921 cgatccctcg ctgatcgagg actttcactg gctcgacgac ccgaagtggg gcgccgcggg
     1981 cagctacctg cacgtcgagg cgaactacca gctgctggtc gagaacctgc tcgacaccac
     2041 ccacctgccc ttcctgcacc cgacctcgct cggcaccgac gccttcgcac gctccgagtt
     2101 cgaggtgacg cgcgaggccg accgcgtcaa ggtgacgcgc tggctgatga acgagctgcc
     2161 ggcgcccttc cacaagaaga tgggcggctt ccccgacggc atgaaggtcg accgctggca
     2221 gatcgccgcc ttctcgccgc cgtgcttcgt caagctcgac gtcggctcgg cccccgccgg
     2281 caccggcgcc cgccagggcg accgcaaggg ccacatgaac atgtggaacc tgaacgcgat
     2341 cacgcccgag acggaaaaga ccgcgcacta cttcttcgcc caggcctaca acgtgagagt
     2401 gaacgagaag tggatgcagg aggtagtgcg cgcccaggtg gtcggcgcct tcctcgagga
     2461 catggcgatg atcaaggcgc agcagaagaa catggacctc ggcgcgagcg cggtggtgaa
     2521 cctcggccag gacaaggcct ggatcgtcat gcgccagatc gtcgagcgcc tggtgaagga
     2581 agaacaacag caggtgcggg cggcatgagc gacgtcgtgc ggcgcgaaga tccggcgcac
     2641 aagggcaagg gcgcctgggt gatcgagcgc gacggggaga tactcgccga gcttggcttt
     2701 accaccgccg gcagccgggt gatcctcgac cacaccgacg tgagcgacgc gctgcgcggc
     2761 acgggctccg gcaagaagct ggtggaagcc gcggtgctct gggcgcgcgg cgcgaacgtc
     2821 aagctgatcc cgctctgccc gttcgccaaa tctgtgttcg acaagacccc ggaaatccgc
     2881 gacgtgctct agagtctaga gcttcgcgct cgccaggcgg gcgccctcgc gcatcgcctg
     2941 gaacttggcc cacaccacgc tcggatgcac ctcggtggtg taggtggcct gcgaggagaa
     3001 gccgcagtcg gcaccggcga tgacgctgtc ggcaccgacc agatttgtat agcgcagcag
     3061 ccgctcggcg atcagctctg gatgctcgac tatgttgctg gcgtgcgtga tcacgccggg
     3121 gatcagcttc ttgccgtcgg cgagcttgac cgtctcccac aggtggtatt cgtgctcgtg
     3181 gcgcgggttc gcggcctcga agctgtaggc gccggcgttc accgccagca tggcatcgac
     3241 cacgtcggcg agcgccgctt cgtggatgcg cggcccttcg ttgatgccgt agcaggtgtg
     3301 aaagcgcacc ttttcctgcg ggatgccccg caggctctcg ttcagcgcct cgacgtacat
     3361 cgccgcgcgg gcgcgccgcc ccttcatgtc gtactcctgg tcggcgaaca gctcggagag
     3421 gaacgggtcg tcgatctgca gcgtaaagcc ggcgtcgacg attgccttgt actcggcgcg
     3481 cagcgccgca ccgactgcgt ggaagtactc ctcctcggtc ttgtagtact cgttcgtgcc
     3541 gaccccgctc ggcgccacgg ccggcatgaa cacaccctta gcgcccgcgg ctgcggcctt
     3601 gaggttggcg atatcccggg cgatggcggc ctcgccgcgg tatttcaccg gcgccgtgca
     3661 caccatcggc actacgggcg ccacggcgcc gccgatcatc gcctgcttga agtactgcgc
     3721 gtagtagtcg gggaaggccg ccacctcggc gtcgaaaaac ttgcgtttca tgccggggcg
     3781 cggcgtgaag ccttccaggc gctcgttcac gtacaggaag aacccgggtt tcgactgctc
     3841 cccgtcggtg acgatatcga tgcccgcctc gagctgcttt cgcacgcatt ccgctaccgc
     3901 cgttttcacg cggccttcat aggcggtctt gtcgtagggc ttgccgcttg ccttcgcttt
     3961 catgaggtcg agtaggtcat gcggccgcgg caggctgc
//
LOCUS       DASYJA010000157         3046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_933564, whole genome shotgun sequence.
ACCESSION   DASYJA010000157 DASYJA010000000
VERSION     DASYJA010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933564"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..1788
                     /locus_tag="VGO02_03325"
     CDS             118..1788
                     /locus_tag="VGO02_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEV7476750.1"
                     /translation="MSNLLDSTRLRFAGAASSAAEDLGQRLEQLNAIGASLSAERDIN
                     RLLESILAAAKTITRADGGTLYRVTEERTLRFEIVRTSSLKYYLGGTTGNPVPFYPIQ
                     LYKDGKPNHSMVAAHAALTGKTVNIPDAYAAEGFDFSGTRAFDAKTGYRSKSFITVPM
                     RNHDHEIIGVLQLINAHDPTSGEVVAFSPSDQRLAESLASQAAVALTNRMLIMQLEQL
                     FESFINLMNSAIDEKSPYTGGHCQRVPVLTMLLADAVNDTREGPLRDFTMSEKDRYEL
                     KIAGLLHDCGKVTTPVHIVDKATKLETIFDRVHLIDTRFEVLKRDAEIDTLRKKMNLF
                     QEKKPMEAADEDKRLRDRLRALDDDRRFLHAANIGSERMQDDDIARVRRIAAYRWRDT
                     AGHEADFLTQDEVKNLTIRAGTLTDDERKIINHHIVATIKMLEALPWPKHLTKVPEYA
                     GGHHERMDGKGYPKGLTREQMSVQARCMGIADIFEALTAKDRPYKKGKTLSESLEILG
                     RMKQNGHVDPDLFDVFVGKQVYRRYAEMFLDPEQIDTVDESKIPGFRS"
     gene            1801..2124
                     /locus_tag="VGO02_03330"
     CDS             1801..2124
                     /locus_tag="VGO02_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476751.1"
                     /translation="MLGWFNTKELDEFADSLVAELLERVPPAGRQLSGAKALQRISRS
                     FGATFTRIDAFARSQRLNVYKKAHFANRIRWALKEAGYPEDFCATMTQELLMHLSPPP
                     REKGK"
     gene            2124..>3046
                     /locus_tag="VGO02_03335"
     CDS             2124..>3046
                     /locus_tag="VGO02_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV7476752.1"
                     /translation="MPQAGPLEAGWEALKRDDLKAAEQIARAALARSAHDGEALYLLG
                     SSLLFQNRFAEALAPLDAALRTAPRRGMGHRLGYCQLALGEFKRAEELLRREVEAYPD
                     LIDAWNALGVALINQSRLQDALEVFLEAARRDPRSAVAHTNVANVLGDLGRGAEALPH
                     LQQAVALDPALADARYNLGVLLHGQKRHEEAIDSLEQALRLAPAMTYALSHLVWNELA
                     ICRWEKLPARIEALRAQVRSAGNVVEPFTFLAVSHSAEEQRLCAERYVQDRLPARPAP
                     LWQGTRYRHDRIRLAYLSADYHEHATAYLAAG"
BASE COUNT          550 a         1088 c          985 g          423 t
ORIGIN      
        1 atgctggcgc agtgcctgga ggacggcatg cgcaccctga agcaggacgg catggagaaa
       61 tgcctgaccg gggtcacgca catcaaggaa gtccgctccg tctgcattaa atagcgaatg
      121 agcaacctcc tcgactccac ccgacttcgt ttcgccggtg cggcgtcgag cgccgccgag
      181 gatctcggcc agcgcctgga gcagctcaac gccatcggcg cctcgctgtc cgcggagcgc
      241 gacatcaacc ggctgctgga atcgatcctc gccgcggcca agaccatcac gcgcgccgac
      301 ggcggcacgc tctatcgcgt gacggaagag cgcacgctgc gctttgagat cgtgcgtacc
      361 agctcgctca agtactacct gggcggcacc accggcaacc cggtgccgtt ctacccgatc
      421 cagctctaca aggacggcaa gccgaaccac agcatggtgg cggcgcacgc cgcgctcacc
      481 ggcaagacgg tgaacatccc cgacgcctac gccgccgagg gcttcgactt ctcgggcacg
      541 cgcgccttcg acgccaagac cggctaccgc tccaagtcct tcatcaccgt gccgatgcgc
      601 aaccacgacc acgagatcat cggcgtgctg cagctgatca acgcgcacga cccgaccagc
      661 ggcgaggtgg tggccttctc cccctccgac cagcgcctgg ccgagtcgct cgcctcgcag
      721 gccgcggtgg cgctcaccaa ccgcatgctg atcatgcagc tcgagcagct gttcgagtcg
      781 ttcatcaacc tgatgaactc ggcgatcgac gagaagtcgc cctacaccgg cggccactgc
      841 cagcgcgtgc cggtgctcac catgctgctg gccgacgcgg tgaacgacac gcgcgagggc
      901 ccgctgcgcg acttcacgat gagcgagaag gaccgctacg agctgaagat cgccggcctg
      961 ctgcacgact gcggcaaggt caccaccccg gtgcacatcg tcgacaaggc gaccaagctg
     1021 gagaccatct tcgaccgcgt gcacctgatc gatacgcgct tcgaggtgct caagcgcgac
     1081 gccgagatcg atacgctgcg caagaagatg aatttgttcc aggagaaaaa gccgatggag
     1141 gccgccgacg aggacaagcg cctgcgcgac cggctgcgtg ccctcgacga cgaccgcaga
     1201 ttcctgcacg cagccaacat cggcagcgag cgcatgcagg acgacgacat cgcgcgcgtc
     1261 aggcgcatcg ccgcctaccg ctggcgcgac accgccggcc acgaggccga cttcctgacg
     1321 caggacgagg tgaagaacct caccatccgc gccggcacgc tgaccgacga cgagcgcaag
     1381 atcatcaacc accacatcgt cgccaccatc aagatgctcg aggcgctgcc ctggcccaag
     1441 cacctcacca aggtgccgga gtacgccggc ggccaccacg agcgcatgga cggcaagggc
     1501 tacccgaagg gcctgacgcg cgagcagatg tcggtgcagg cgcgctgcat gggcatcgcc
     1561 gacatcttcg aggcgctcac cgccaaggac cggccctaca agaagggcaa gacgctgtcc
     1621 gagtcgctcg agatcctcgg ccgcatgaag cagaacggcc acgtcgaccc ggacctgttc
     1681 gacgtcttcg tgggcaagca ggtgtaccgg cgctacgccg agatgttcct cgaccccgag
     1741 cagatcgaca cggtcgacga gagcaagatt cccggtttcc ggtcctagcg gccgcgcctg
     1801 atgctgggct ggttcaacac caaagagctg gacgagttcg ccgattcgct ggtcgccgag
     1861 ctgttggaac gcgtgccgcc cgcgggccgc cagctctccg gcgcgaaggc gttgcagcgc
     1921 atcagccgca gcttcggcgc gaccttcacg cgcatcgacg ccttcgcccg ctcgcagcgc
     1981 ctgaacgtct acaagaaggc gcatttcgcc aaccgcatcc gctgggcgct gaaggaggcc
     2041 ggctacccgg aggatttctg cgccacgatg acgcaggagc tgctcatgca cctgtcgccc
     2101 ccgccgcgcg agaagggcaa gtagtgcccc aggccgggcc gctggaggcg ggctgggaag
     2161 cgctcaagcg cgacgatctg aaggccgccg agcagatcgc gcgcgccgcg ctggcgcgct
     2221 cggcgcacga cggcgaggcg ctgtacctcc tcggctccag cctgctcttc cagaaccggt
     2281 tcgcggaggc gctggcaccg ctcgatgcgg cgttgcgcac ggcgccacgc cgcgggatgg
     2341 gccaccgcct gggctattgc cagctcgcgc tgggcgaatt caagcgggcc gaggagctgc
     2401 tgcggcgcga agtcgaggcc tatcccgacc tgatcgacgc gtggaatgcg ctcggcgtcg
     2461 cgctgatcaa ccagtccagg ctgcaggatg cgctcgaggt gttcctcgag gcggcgcggc
     2521 gcgatccgcg ctcggcggta gcgcatacga acgtggccaa cgtgctcggc gacctcgggc
     2581 gcggcgcgga agcgctgcct cacctgcaac aggccgtcgc gctcgacccc gcgctcgccg
     2641 acgcccgcta caacctcggc gtgctgctgc atggccagaa gcgccacgag gaggcgatcg
     2701 acagcctgga gcaggcgctg cgcctggcgc cggcgatgac ctacgccctc agccacctgg
     2761 tgtggaacga gctcgcgatc tgccgctggg agaagctgcc ggcgcgcatc gaggcgctgc
     2821 gcgcgcaggt gcgcagcgcc ggcaatgtgg tcgagccttt cactttcctc gcggtctcgc
     2881 actcggccga ggagcagagg ctgtgcgccg agcgctacgt ccaggacagg cttccggcgc
     2941 gccccgcgcc gctatggcag ggcacgcgct accgccacga ccgcatccgc ctcgcgtacc
     3001 tgtcggccga ctatcacgag cacgcgactg cctaccttgc cgccgg
//
LOCUS       DASYJA010000158         2126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_206520, whole genome shotgun sequence.
ACCESSION   DASYJA010000158 DASYJA010000000
VERSION     DASYJA010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206520"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VGO02_03340"
     CDS             <1..319
                     /locus_tag="VGO02_03340"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV7476753.1"
                     /translation="AKRLEDSGDGPFKSFPRPESAILCGDFNMKPDDPLHDKIGLVDA
                     WKALNPGKPHPATFRVHEAGETPYCCDYVFVTPDLAPRLRAIRVDGATQASDHQPVIV
                     ELA"
     gene            complement(316..813)
                     /locus_tag="VGO02_03345"
     CDS             complement(316..813)
                     /locus_tag="VGO02_03345"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEV7476754.1"
                     /translation="MLALKRLLLFVVLLAAGAAAGVALHTALTQGDTMFAGKRPQNLG
                     HGTGRLAPCKSTPNCVSSQADPKDEQHYIAPISLKSDPIAAVRKAVESLPRTSVVQVE
                     PDYLYAEFRSKLMGFVDDVEFLADRAKGVVHVRSAARLGRRDFGVNRERIEQLRSILE
                     KRIRS"
     gene            849..1157
                     /locus_tag="VGO02_03350"
     CDS             849..1157
                     /locus_tag="VGO02_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476755.1"
                     /translation="MQISAQQLADLLTGIARAQAAIIQGLENEMAGVRSGRIIPALHN
                     VAHLRDHPNPTLVDLPSRVLLGTLGRNVPDTAGLVRDLERLFGEAAAPAAALDFSKPA
                     "
     gene            complement(1154..>2126)
                     /locus_tag="VGO02_03355"
     CDS             complement(1154..>2126)
                     /locus_tag="VGO02_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEV7476756.1"
                     /translation="GLSERLLVSHYVNNYGGALRRLNAIRARLAALDWASAPGFEING
                     LKREELIASGSVILHEIYFESLGGHGDKPPTGLEEPPVDLAQALERDFGSVMAWRAEF
                     VAMAKALAGGSGWAILAWSDRLGRLVNHWAADHAHGLQGATPILAIDMYEHAYHLDFG
                     AKAAAYVEQVMLNLNWQRIAARYRLAIAESAGEDKLFVPYGAPEQEEARISTEELKAA
                     LEDEVDRRPVLLDLCLPKDLPRRSDMLAGATMHAPAALPRWIEELPRDRPIAVYCICG
                     FQVSGTAVTELRRRGYDARALIGGITAWHATGGATVPMDTSTYEKDV"
BASE COUNT          363 a          719 c          690 g          354 t
ORIGIN      
        1 ggcaaagcga ctggaagaca gcggggacgg tcccttcaaa tccttccccc ggcccgaaag
       61 cgcgatcctg tgcggcgact tcaacatgaa gccggacgac ccgttgcacg acaagattgg
      121 attggtcgat gcgtggaagg cgctgaaccc cggcaaaccg catccggcca cattccgcgt
      181 gcacgaagcg ggcgagacgc cgtattgctg cgactatgtt ttcgtcacgc ccgatctcgc
      241 gccgcggctg cgcgcgatcc gcgtcgacgg cgcgacgcag gcctccgacc accagccggt
      301 gatcgtcgag ctcgcctagc tcctgatgcg cttctcgagg atcgagcgca gctgctcgat
      361 gcgctcacgg ttgacgccga aatccctgcg gcccaggcgc gcggccgagc gcacgtgcac
      421 cacgcccttg gcgcggtcgg cgaggaactc gacgtcgtcg acgaagccca tcagcttgct
      481 gcggaactcg gcgtagagat agtcgggctc gacctgcacc acgctggtcc gcggcagcga
      541 ctccaccgcc tttctcacgg cggcgatggg atcgctcttc agcgagatcg gcgcgatgta
      601 gtgctgctcg tctttcggat cggcctgcga cgagacgcag ttgggggtgc tcttgcaggg
      661 cgcgaggcgc cccgtgccgt gacctagatt ctgcgggcgc ttgccggcga acatggtgtc
      721 tccctgggtg agcgcggtgt gcagcgcgac gcccgccgct gcgccggcgg cgagcaggac
      781 gacgaagaga agcagtcgct tgagtgctaa catcgacgca agcctatcgt acccggggag
      841 gaacctccat gcagatcagc gcccagcagc tcgccgacct tctcacgggc atcgcccgcg
      901 cgcaggcggc gatcatccag ggactcgaga acgagatggc cggcgtgcgc agcggcagga
      961 tcatcccggc gctgcacaac gtagcgcacc tgcgcgatca tccgaatccg acgctcgtcg
     1021 acctgccctc gcgcgtgctg ctcggcacgc tcgggcgcaa cgtcccggac accgccgggc
     1081 tggtgcgcga tctggagaga ctgttcggcg aggcggcggc gccggccgcg gccctcgact
     1141 tcagcaagcc ggcctagacg tccttctcat aggtagaggt atccatcggc actgtcgcgc
     1201 cgccagtggc atgccaagcg gtaatcccgc caataagcgc cctcgcgtca tagccccgcc
     1261 gccgcaattc ggtcacggcg gtgccgctga cctgaaatcc gcagatgcaa taaaccgcga
     1321 tcggccggtc tcggggcagt tcctcgatcc agcgcggcag cgcggcgggg gcatgcatcg
     1381 tggcgcccgc cagcatgtcg ctgcggcgcg gcaagtcctt gggcaggcac aggtccagaa
     1441 gcaccggccg acgatcaacc tcatcctcga gagccgcctt cagttcttcg gtcgaaatcc
     1501 gcgcttcttc ctgctctggt gcgccgtaag gcacgaagag cttgtcctct cccgcacttt
     1561 cggcgattgc aaggcgatac cgcgccgcga tccgctgcca attgaggttg agcatcacct
     1621 gctcgacata ggctgctgcc ttcgcgccga agtcgagatg gtaggcatgc tcgtacatgt
     1681 ctatcgcgag gatcggagta gcgccctgca ggccgtgcgc gtggtctgct gcccaatggt
     1741 tgaccagcct gcccaggcgg tctgaccagg cgaggatcgc ccagccggaa ccgcctgcca
     1801 gcgcctttgc catggcgacg aattcggcgc gccatgccat cacgctgcca aaatcgcgct
     1861 cgagcgcctg agcgaggtcg acgggaggtt cttcaagccc ggtgggcggc ttgtcgccat
     1921 gcccgccgag cgattcgaaa tagatctcgt gcaggatcac agagcccgag gcaatcagct
     1981 cttcgcgctt gaggccgttg atctcgaagc cgggcgcgct tgcccagtcg agagcggcaa
     2041 gacgcgctct gatggcgttc agccgccgca gcgcgccgcc gtagttgttt acgtaatggc
     2101 tcacgagcaa tcgttccgag agcccg
//
LOCUS       DASYJA010000159         1972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_752300, whole genome shotgun sequence.
ACCESSION   DASYJA010000159 DASYJA010000000
VERSION     DASYJA010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752300"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="VGO02_03360"
     CDS             <1..1004
                     /locus_tag="VGO02_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEV7476757.1"
                     /translation="RNPTRDALQDCLAALEGGAAGFAFSSGMAATATVLELLDAGSHV
                     VAMHDLYGGSYRIFERVRKRSAGLQTTFVDLSRPENLEKAIAPNTRMVWIETPTNPLL
                     HLVDLKAVAGIARRHKLLTVCDNTFASPWIQRPLELGCDIVVHSTTKYINGHSDVIGG
                     AAVVRSPELKEKLAFLQNAVGGVPSPFDAFLTLRGIKTLAVRMERHCANASRIASYLE
                     QHPKISRVLYPGLPSHPQHALAQRQMNGRYGGMVTAVIKGGLPASRRFLERCKLFTLA
                     ESLGGVESLIEHPAIMTHASLPEDVRAGLGIDDGLVRLSVGIENVDDLLAELEEALK"
     gene            1001..1819
                     /locus_tag="VGO02_03365"
     CDS             1001..1819
                     /locus_tag="VGO02_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV7476758.1"
                     /translation="MNLRLGDMAVHRLGYGAMRVLGPNVWGEPKDRPGALRLFRRAVE
                     LGVNFFDTAESYGPQTDEVLIAEALHPYPAGFVIGTKGGLLRPSPGRWDSDARPEKLK
                     KDLDGSLKRLRLERIDLYQLHAPDPKVPLEDSLGAIVDAQKAGKVRHIGVSNVNAKEL
                     ERARRVANIVSVQNRYSIGDRNSEDVLKACEKLGIAFLPWYPLGAGSALTNKKILSIS
                     KKINATPVQVAIAWLLAKSPVMLPIPGTSSIAHLEENMKAASLTLSAEDLALLG"
BASE COUNT          345 a          686 c          625 g          316 t
ORIGIN      
        1 cccgcaaccc gacgcgcgat gccctgcagg actgcctcgc cgcgctcgag ggcggcgcgg
       61 ccggctttgc gttctcctcc ggcatggcgg ctaccgctac ggtgctcgag ctgctcgacg
      121 ccggctcgca cgtcgtcgcg atgcacgacc tctacggcgg cagctaccgg attttcgagc
      181 gggtgcgcaa acgcagcgcc ggtctgcaga cgacttttgt ggatctttcc aggccggaga
      241 acctggaaaa agcaatcgcg ccgaataccc ggatggtgtg gatcgagacg cctaccaatc
      301 cgctgctgca cctcgtcgac ctgaaggcgg tcgccggcat cgcgcgacgc cacaagctgc
      361 tcacggtctg cgacaacacc ttcgcctcgc cgtggatcca gcgcccgctc gagctcggct
      421 gcgacatcgt cgtgcactcc accaccaagt acatcaacgg gcactccgac gtgatcggcg
      481 gcgccgccgt ggttcgcagc ccggaactga aggagaagct ggccttcctg cagaacgccg
      541 tgggcggcgt gccgagcccg ttcgacgcct tcctgacgct gcgcggcatc aagaccctcg
      601 ccgtgcgcat ggagcgccat tgcgcgaacg cttcgcggat tgcttcctat ctcgaacagc
      661 acccgaaaat ttcgcgcgtc ctctacccgg gcctccccag ccatccccag cacgcgcttg
      721 cgcagcggca gatgaacggc cgctacggcg gcatggtgac ggcggtgatc aagggcgggt
      781 tgccggcgtc caggcgcttc ctggagcgct gcaagctgtt cacgctggcc gagagcctcg
      841 gcggcgtcga gagcctgatc gagcacccgg ccatcatgac gcacgcatcg ctgccggagg
      901 acgtgcgcgc cggcctgggc atcgacgacg gcctggtcag gctgtcggtc ggcatcgaga
      961 acgtggatga cctgcttgcc gagctggaag aggcgctgaa atgaacctcc ggctcggcga
     1021 catggcggtc caccggctcg gctacggggc gatgcgcgtg ctcggcccga acgtctgggg
     1081 tgaaccgaaa gaccggcctg gcgcgctgcg actgttccgc cgcgccgtcg agctcggggt
     1141 gaacttcttc gacaccgcag agagttacgg cccgcagacg gacgaggtcc tgatcgccga
     1201 agcgctccat ccctaccccg cgggcttcgt catcggcacc aagggcggcc tgctgcggcc
     1261 gagccccggg cgctgggatt ccgatgcccg ccccgagaag ctgaaaaagg acctggatgg
     1321 cagcctgaag cggctgcgcc tggagcgcat cgacctctac cagctgcacg ccccggatcc
     1381 gaaggtgccg ctcgaggact ccctcggcgc gatcgtcgac gcgcagaaag ccggcaaggt
     1441 gcgccacatc ggggtttcaa acgtcaatgc aaaagagctc gaaagagcga gacgcgtggc
     1501 gaacatcgtc tcggtacaga accgctacag catcggcgac cgcaattcgg aagacgtgct
     1561 gaaggcgtgc gagaagctcg gcatcgcctt cctgccctgg tatccgctgg gcgcaggttc
     1621 tgctttgacg aacaaaaaaa ttctttccat ctcgaaaaaa atcaatgcaa ctccggtgca
     1681 agtggccatc gcctggctgc tggcgaagtc gccggtcatg ttgccgatcc ccggcacctc
     1741 ctcgatcgcg cacctcgagg agaacatgaa agccgcctcg ctcacgctga gtgccgagga
     1801 cctcgccctg ctaggctagc gccgtgcgcc tgttgttcct gctcctgcct ctgctcgcca
     1861 ccgccgccca cgcgcaggat ccgcgcgtca cgcagtgcat cgcccagttc ccgccgggcg
     1921 gcgcccgcgc gcaatgcgtg acgccctggc tcgacgacat cgtcatgcag aa
//
LOCUS       DASYJA010000160         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_933696, whole genome shotgun sequence.
ACCESSION   DASYJA010000160 DASYJA010000000
VERSION     DASYJA010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933696"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VGO02_03370"
     CDS             complement(<1..353)
                     /locus_tag="VGO02_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476759.1"
                     /translation="MTPERAPRPAGLGDLLGRLLGEARQLVADYSLLAVLDARRAALQ
                     LAWLLSSGLVVAVLLVTAWLALVVSAMVWLFQERLSWPVALAFGALLNIAGAGALLWW
                     MRHLLEEKPFTALLR"
     gene            complement(367..618)
                     /locus_tag="VGO02_03375"
     CDS             complement(367..618)
                     /locus_tag="VGO02_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476760.1"
                     /translation="MESTRTLDKNIDRMSQSAHRAVDTAASTASSMAERAGQKGEELM
                     EMKDNWVEGAADYVREHPIAALGIAAAAGYLFSVISRWR"
     gene            complement(660..>1556)
                     /locus_tag="VGO02_03380"
     CDS             complement(660..>1556)
                     /locus_tag="VGO02_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEV7476761.1"
                     /translation="VGESGTGKELAAQTIHDLSRRKKSPFLPLNCGAVSPQLIESELF
                     GHEKGSFTGADRQHKGYFERAHGGSLFLDEITEMPQELQVKLLRVLETGSFTRVGTTQ
                     PISADVRLIAATNRNPEAAVADGKLREDLYHRLNVFPLAMPPLRDRASDIELLAQHFL
                     EVLNKQEGTGKSFAPATVAALYAHSWPGNVRELKNYVQRASILADDVIGADLAPAAVA
                     APESAPILTVRVGSTLDEVSRRLIEATLAECRSKRKAADMLGISLKTLYNRLAIYKAE
                     PSAEDDEELQTANAAGERRQAA"
BASE COUNT          212 a          543 c          549 g          252 t
ORIGIN      
        1 tgccgcagca gcgcagtgaa cggtttctcc tccagcaggt ggcgcatcca ccagagcagt
       61 gcgccggcgc cggcgatgtt cagcagcgcg ccgaaggcga gcgcgaccgg ccaggacagg
      121 cgttcctgga acagccacac catggcggac accaccagcg ccagccacgc ggtcaccaag
      181 agcactgcca cgaccagccc cgaggacagc agccacgcca gctgcagcgc cgcgcggcgc
      241 gcatcgagca ccgcgagcag cgagtagtcg gcgaccagtt gccgcgcctc gcccagcagg
      301 cggccgagca ggtcgccgag ccccgcgggg cgcggcgcgc gttccggcgt catcctggcg
      361 cggcgcctaa cgccagcgcg agatcacgct gaacaggtag ccggcggcgg cggcgatgcc
      421 gagcgcggcg atcgggtgct cgcgcacgta gtcggcggcg ccttcgaccc agttgtcctt
      481 catctccatc agctcctcgc ctttctggcc ggcgcgctcg gccatcgagc tcgccgtcga
      541 ggcggcggta tcgaccgcgc ggtgagcgct ctggctcata cggtcgatgt tcttgtccag
      601 ggtacgggtg ctttccatga ctctctctcc tttgaggggt gacaaatcct tagcaactgc
      661 tatgccgcct gccggcgctc gcccgccgcg ttggcggtct gcagctcctc gtcgtcctcg
      721 gcggagggct cggccttgta gatcgcgagc cggttgtaga gcgtcttcag gctgatgccg
      781 agcatgtcgg cggccttgcg cttcgagcgg cactcggcga gggtcgcctc gatcaggcgg
      841 cggctcactt cgtcgagcgt gctgccgacc cgcacggtga ggatcggggc cgactcgggc
      901 gccgccaccg ccgccggggc gaggtcggcg ccgatcacgt cgtcggcgag gatcgaggcg
      961 cgctgcacgt agttcttcag ctcgcgcacg ttgcccggcc agctgtgcgc gtacagcgcg
     1021 gcgacggtcg ccggcgcgaa gctcttgccg gtgccctcct gcttgttcag cacctcgagg
     1081 aagtgctgcg ccaggagctc gatgtccgag gcgcggtcgc gcagcggcgg cattgccagg
     1141 gggaacacgt tgagccggtg gtacaggtcc tcgcgcagct tgccgtcggc gacggccgcc
     1201 tccgggttgc ggttggtggc ggcgatcagg cgcacgtcgg cgctgatcgg ctgcgtcgtg
     1261 ccgacgcgcg tgaagctgcc ggtctccagc acgcgcagca gcttcacctg cagctcctgc
     1321 ggcatctcgg tgatctcgtc gaggaacagc gagccgccgt gcgcgcgctc gaagtagccc
     1381 ttgtgctggc ggtcggcgcc ggtgaacgag cccttctcgt ggccgaacag ctcgctttcg
     1441 atgagctgcg gcgataccgc gccgcagttc agaggaagga agggggactt cttccgcctg
     1501 ctcaggtcat ggatggtctg cgccgccagc tccttgccgg tgccgctctc gcccac
//
LOCUS       DASYJA010000161         5791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3022360, whole genome shotgun sequence.
ACCESSION   DASYJA010000161 DASYJA010000000
VERSION     DASYJA010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3022360"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VGO02_03385"
     CDS             <1..401
                     /locus_tag="VGO02_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV7476762.1"
                     /translation="ARHQLGDAPFLLVNSDIYCEYDFSALRSVSLGKNLAHIVLVPNP
                     AHHMNGDFILQSGMVGNDGAPRYTYAGVAVVSPGLVSGVNPGDKVPLGPLLRQAAGER
                     RLSGELFRGVWTDVGTIERLTELRTLLASR"
     gene            398..1714
                     /locus_tag="VGO02_03390"
     CDS             398..1714
                     /locus_tag="VGO02_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEV7476763.1"
                     /translation="MNLTDNPAIHRRRRERLAQAIGAGVAVVPTAPERVRNRDTHYPY
                     RFDSHFYYLTGFAEPEAVLVLVAGKSILFCREKNDEREVWDGFRYGPAAARERFGFDE
                     AHPIGALDGMLAQLLENQPALLYPVGADAGWDARAMGWLNAVRAKARAGTAAPDRVQD
                     VRTLIDEMRLVKDAQELATMRRAARITAGAHRRAMQQARPGRNEYEIEAELLHEFRRN
                     GAQFPAYWPIVAGGANACVLHYVSNDAPLREGELLLIDAGCELDGYAADLTRTFPVSG
                     RFSGAQRALYEVVLAAQLAAIATVKPGRAWNEPHDAAVRVLAQGMLDLKLLSGTLDAV
                     LEKETYKRFYMHRTGHWLGLDVHDAGEYKQAGKWRTLAPGMTLTVEPGLYVRPADDVP
                     QALHHLGIRIEDDVAVTEGGCEVLTAEAPKTVDDIEALMRDARGRN"
     gene            1695..2747
                     /locus_tag="VGO02_03395"
     CDS             1695..2747
                     /locus_tag="VGO02_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEV7476764.1"
                     /translation="MPAAAIDVLIRGAGPVGCALALALADSHLKVRILEKKANPAGFR
                     PITLSYASRLILERIGAWSELAATPIDTILVSQSGGFGRTQMDAADAGVPHLGYVLDY
                     SALAAALRAKAARFFTDEEAAAECTVHAEGVARDAEETRYSQDALVGLLTAEPAAQAA
                     AFERFTPEGPLALLPLEGRYAVVWSARPQRAAELAAMREAQFLAALAAAAGRRAGRLV
                     AVEARSVHSLALRVRPARVSERAVYIGNAAQTLHPVAGQGLNLGLRDAWELAQMWRDA
                     QDPGDAALLARYAARRRLDAAATIRVTDWMAKGFTGTNRLSRAARGVALTALDVLPGP
                     RRFFARRMIYGPSALP"
     gene            2776..3744
                     /gene="dusB"
                     /locus_tag="VGO02_03400"
     CDS             2776..3744
                     /gene="dusB"
                     /locus_tag="VGO02_03400"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEV7476765.1"
                     /translation="MKIGAHPLRNGLLVAPMAGITDRPFRRIARRMGAALAVSEMVSS
                     RPELRESRKTRLRQDHAGEPGPISVQIAGADPAMLADAARYNAGLGADIIDINMGCPA
                     KKVCNAQAGSALLEDEALVARILEAVVAAAGVPVTLKIRTGPAPERRNALRIAHIAED
                     AGIQALAIHGRSRACMFEGRAEYETIAEVKTRVRIPVIANGDVESPEEAKRVLELTGA
                     DGIMIGRAAQGRPWLFRSIARYLETGELLAAPAAPEIGALLLEHLEGLYQLYGDEQGA
                     RVARKHIGWTVREMAGGEDFRRQVIRIDDAAAQRCAVNDYFAKLAA"
     gene            3741..3980
                     /locus_tag="VGO02_03405"
     CDS             3741..3980
                     /locus_tag="VGO02_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7476766.1"
                     /translation="MSRSNELADCVKRSLERYFKDMDGEKPTSIYEMVLKNIEKPMIE
                     TVLGQAKGNQTLAAEMLGINRNTLRSKMQQLRIKG"
     gene            4001..5551
                     /gene="purH"
                     /locus_tag="VGO02_03410"
     CDS             4001..5551
                     /gene="purH"
                     /locus_tag="VGO02_03410"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEV7476767.1"
                     /translation="MQRALVSVSEKTGVVDFARALAALGIQIISTGGTAQLLAKEGVA
                     VTEVSAHTGFPEMLDGRVKTLHPKIHGGLLARRDDAAHMKALRGAGIETIDLLVVNLY
                     PFQATVADPECRFEDAVENIDVGGPAMLRAAAKNHADVAVVVDPADYPRVLEEIHSSG
                     KVSDATRFELAKKVFAHTSAYDGAIANYLSSLDTQRKRGEYPDTLSLQFRKLQDLRYG
                     ENPHQSAAFYRDDRPVAGALAGYRQLQGKELSYNNIADADAAWECVKSFAEPACVIVK
                     HANPCGAALGTTLLEAYNKAFATDPTSAFGGIIAFNRALDAPTAEALGKQFAEVVIAP
                     RLEPSVKEVFAKKANVRVLEIPLAHDVQAHDYKRVGGGVLVQTTDFHPVDRKALRIAT
                     KKQPTESQWSDLLFAWRVAQFVKSNAIVFCRGGATLGVGAGQMSRVDSARIASIKAEN
                     AGLALAGSVVASDAFFPFRDGLDVAADAGAVAVIQPGSSMRDEEVIAAADEHGIAMIF
                     TGTRHFRH"
     gene            5552..>5791
                     /locus_tag="VGO02_03415"
     CDS             5552..>5791
                     /locus_tag="VGO02_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003272623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEV7476768.1"
                     /translation="MKLLVIGSGGREHALASKLAHSPKVQKVFVAPGNGGTALESGLE
                     NIALTEIPKLIEFAKKEHIHLTVVGPEAPLAAGIVD"
BASE COUNT          988 a         2003 c         1928 g          872 t
ORIGIN      
        1 aggcgcgcca ccagctcggc gacgcgccgt tcctgctcgt caactccgat atctactgcg
       61 aatacgattt ttccgccctg cgcagcgttt ctctcggcaa gaacctggcg cacatcgtgc
      121 tggttcccaa tcccgcccac cacatgaacg gcgacttcat tctccagtcg ggcatggtcg
      181 gcaacgacgg cgcgccgcgc tacacttatg caggcgtcgc ggtcgtgtcg ccaggtttgg
      241 tctccggggt gaaccccgga gacaaagtgc cgctcggacc gctgctgcgc caagcggcgg
      301 gtgagcgcag gctgtcgggt gagctgttcc gtggcgtgtg gaccgacgtc ggcacgatcg
      361 agcggctgac cgaactcagg accttgctgg catcaagatg aacctcaccg acaaccccgc
      421 aatacaccgc cgccgcagag agcggctggc gcaggccatt ggcgccggcg tcgccgtggt
      481 gcccaccgcg cccgagcgcg tgcgcaaccg cgacacgcat tacccctacc gcttcgacag
      541 ccacttctac tacctcaccg gcttcgcgga gcccgaagcc gtacttgttt tggttgccgg
      601 gaaatcgatt cttttttgtc gcgagaagaa cgacgagcgc gaggtgtggg acggctttcg
      661 ctacggcccg gccgcggcgc gcgagcgctt cggcttcgac gaggcgcatc cgatcggcgc
      721 gctcgacggc atgctcgccc agctgctcga gaaccagccg gcgctgctct atccggtcgg
      781 cgccgacgcc gggtgggacg cgcgcgccat gggctggctg aacgcggtgc gcgccaaggc
      841 gcgcgccggc actgccgcgc ccgaccgcgt gcaggacgtg agaaccctga tcgatgagat
      901 gcggctagtg aaggacgcac aggagctcgc gaccatgcgg cgtgcggcgc gcatcaccgc
      961 gggcgcgcac cgccgggcga tgcagcaggc gcggcccggc aggaacgagt acgagatcga
     1021 ggccgagctg ctgcacgaat tccggcgcaa cggagcgcag ttccccgcct actggccgat
     1081 cgtcgccggc ggcgcgaacg cctgcgtgct gcactatgta tccaacgatg cgcccctgcg
     1141 cgagggcgag ctgctgctga tcgacgccgg ctgcgagctc gacggctacg ccgccgacct
     1201 gacgcgcacc tttccggtca gcggccgctt cagcggcgcg cagcgcgcgc tctacgaagt
     1261 ggtgctcgcg gcgcagctcg cggcgattgc gacggtgaag cccggcaggg cctggaacga
     1321 gccgcacgac gccgcggtgc gcgtgcttgc gcagggaatg ctcgacctca agctcctttc
     1381 cggcacgctc gacgccgtgc tcgagaagga gacctacaaa cgcttctaca tgcaccgcac
     1441 cggccactgg ctcgggctcg acgtgcacga cgctggcgag tacaagcagg ccggcaaatg
     1501 gcgcacgctc gccccgggca tgaccctcac cgtcgagccc ggactctacg tgcgccccgc
     1561 cgacgacgtg ccgcaagcgc tgcaccacct cggcatacgc atcgaggacg atgtggccgt
     1621 aaccgagggc ggctgcgagg tgcttaccgc ggaggcgccg aagacggtcg acgacatcga
     1681 agccctgatg cgcgatgccc gcggccgcaa ttgatgtcct gatccgggga gcaggcccgg
     1741 tgggttgcgc gctcgcgctc gccctcgcgg attcgcattt aaaagtaaga atactggaaa
     1801 aaaaggcgaa ccctgccggt tttcgcccga tcaccctctc ctatgcgagc cggctgatcc
     1861 tcgagcgcat cggggcatgg agcgagctcg ccgccacgcc gatcgatacg atcctggtgt
     1921 cgcagtccgg cggcttcggc cgcacgcaaa tggacgcggc cgatgccggg gtgccgcatc
     1981 taggctacgt gctggactat tccgccctgg cggctgccct gcgggccaag gcggcgcggt
     2041 tttttactga cgaagaagcc gcggcagagt gcaccgtgca cgccgaaggc gtcgcgcgcg
     2101 atgcagagga aacccgatac tcacaggatg ccctggtcgg gctcttgaca gccgaacccg
     2161 cagcccaggc cgccgccttc gagcgcttca ctcctgaagg tccgctcgcc ctgctgccgc
     2221 tcgagggccg ctatgccgtg gtctggagcg cgagaccgca gcgcgcggca gagcttgccg
     2281 ccatgcgcga ggcgcagttc cttgcggcgc tcgcggcggc ggccggacgc cgggcgggac
     2341 ggctggtggc tgtcgaggca cgctcggtgc actcgcttgc gctgcgcgtg cgaccggcgc
     2401 gggtcagcga gcgggcggtg tacatcggca acgcggcgca gacgctgcac ccggtcgcgg
     2461 gccagggcct caatctcggc ctgcgcgacg cgtgggagct agcgcaaatg tggcgggatg
     2521 cgcaagaccc cggcgacgcg gcgttgctcg cgcgctacgc cgcgcgccgc cgcctcgacg
     2581 ccgccgcaac gatccgcgtc accgattgga tggcaaaggg atttaccggg acgaaccgcc
     2641 tgtcgcgcgc cgcccgcggc gtcgcgctga ccgcgctcga cgtgctgccg ggaccgcggc
     2701 gcttcttcgc gcggcgcatg atttacgggc cgtctgcgct gccgtaaaag attcgatata
     2761 aaattcccat cccccatgaa gatcggcgcc catccgctgc gcaacggctt gctcgtcgct
     2821 ccgatggccg gcattaccga tcgccccttc cggcgcatcg cgcggcgcat gggcgccgcc
     2881 ctcgccgtct ccgagatggt ctcgtcgcgc cccgagctgc gcgagtcgcg caagacgcgg
     2941 ctgcgccagg accatgccgg cgagcccgga ccgatctcgg tgcagatcgc cggagccgat
     3001 cctgcaatgc tggccgacgc ggcgcgctac aacgcgggcc tgggcgcgga catcatcgac
     3061 atcaacatgg gctgcccggc gaagaaggtc tgcaacgccc aggccggctc ggcgctgctg
     3121 gaagacgagg ccctcgtggc gcgcatcctg gaggcggtgg tggctgcggc cggcgtgccg
     3181 gtgacgctca agatccgcac cggacccgcg cccgagcggc gcaatgcctt gcgcattgcg
     3241 catattgctg aagacgccgg catccaggcc ctcgccatcc atggccgcag ccgagcctgc
     3301 atgttcgagg gccgcgcgga atacgagacc atcgccgagg tcaagacgcg cgtgcgcatc
     3361 ccggtcatcg ccaatggcga tgtcgaaagc cccgaggagg cgaagcgcgt gctcgagctg
     3421 accggcgccg acggcatcat gatcggccgc gccgcccagg gccggccctg gctgttccgc
     3481 tccatcgccc gctatctgga aaccggcgag ctgctcgccg ccccggccgc gcccgaaata
     3541 ggtgcgctgc tgctggagca cctcgagggc ctgtaccagc tctatggcga cgagcagggc
     3601 gcgcgcgtcg cgcgcaagca catcggctgg acggtgcgcg agatggctgg cggcgaggat
     3661 tttcgccgcc aggtgatccg catcgacgat gcggccgcgc agcgctgcgc agtgaacgac
     3721 tatttcgcta aactcgccgc atgagccgaa gcaacgagct cgccgactgc gtcaagcgct
     3781 cgctcgagcg ctacttcaag gacatggacg gcgagaagcc cacctcgatc tacgagatgg
     3841 tgctgaagaa catcgagaag ccgatgatcg agacggtgct cggccaggcc aagggcaacc
     3901 agacgctcgc cgccgagatg ctcggcatca accgcaacac gctgcgcagc aagatgcagc
     3961 aactgcgtat caagggttag cgcccaagtt agtggggaaa atccagcggg ccctcgtcag
     4021 cgtctcggag aagaccggcg tggtcgactt cgcccgggcg ctcgccgcgc tcggcatcca
     4081 gatcatctcc accggcggca ccgcgcagct cctcgccaag gaaggcgtcg ccgtcaccga
     4141 ggtctccgcg cacaccggct ttccggaaat gctcgacggc cgcgtcaaga ccctgcaccc
     4201 gaagatccac ggcggcctgc tcgcgcggcg cgacgatgcg gcgcacatga aggcgctgcg
     4261 cggcgccggc atcgagacca tcgatctgct ggtggtcaat ttgtatccgt tccaggctac
     4321 ggtggccgac cccgaatgcc gcttcgagga tgcggtggag aacatcgacg tcggcgggcc
     4381 cgcgatgctg cgcgcggcgg ccaagaacca tgccgacgtc gccgtggtcg tcgacccggc
     4441 cgactaccca cgcgtcctgg aggaaattca ttcttcagga aaagtttctg atgcaacccg
     4501 cttcgagctc gcgaagaagg tcttcgccca tacctcggcc tacgacggcg cgatcgcgaa
     4561 ctatctctct tcgctcgaca cgcagcgcaa gcgcggcgaa tacccagaca cgctgagcct
     4621 gcagtttcgc aaactgcagg acctgcgcta cggcgagaac ccgcaccaga gcgcggcgtt
     4681 ctatcgcgac gatcgtccgg tggccggcgc gctcgccggg tatcggcagc tccagggcaa
     4741 ggagctctcc tacaacaaca tcgccgacgc cgacgcggcc tgggaatgcg tcaagagctt
     4801 cgccgagccg gcctgcgtca tcgtcaagca cgccaacccc tgcggcgccg cgctcggcac
     4861 gacgctcctc gaggcctaca acaaggcctt cgcgaccgat ccgacgtcgg cgttcggcgg
     4921 catcattgct ttcaatcgcg cgctcgacgc gccgaccgca gaagcgctcg gcaagcaatt
     4981 cgccgaagtg gtgatcgcgc ctcgcctcga accttcggtg aaggaggttt tcgcaaagaa
     5041 agcgaacgtc cgagtactcg agatacctct cgcgcatgac gttcaggcgc acgactacaa
     5101 gcgcgtcggc ggcggcgtgc tggttcagac caccgatttt catcccgtgg acaggaaggc
     5161 gttgcggatc gccaccaaaa aacagcccac agagtcgcag tggagcgatc ttctgttcgc
     5221 ctggcgcgta gcgcagtttg tgaaatcgaa cgccatcgtg ttttgccgcg gcggcgcgac
     5281 gctcggcgtc ggcgccgggc agatgagccg cgtcgacagc gcgcgcatcg catcgatcaa
     5341 ggccgagaac gccggcctcg cactcgccgg ctcggtggtc gcttccgacg ccttcttccc
     5401 gttccgcgac gggctcgacg tagcggcgga cgccggcgcc gtggcagtga tccagccggg
     5461 ctccagcatg cgcgacgaag aggtgatcgc ggccgccgac gaacatggca tcgcgatgat
     5521 cttcaccggg actcggcatt tccggcactg aatgaagctc ctcgtcatcg gctcgggcgg
     5581 tcgggaacat gcgctggctt cgaagctcgc gcacagcccg aaggtgcaga aagtgttcgt
     5641 cgcgccgggg aacggcggca cggccctgga gagcggcctc gaaaacatcg cgctcacgga
     5701 gattccgaag ctcatcgagt tcgcgaagaa ggagcacatc catctcacgg tggtcgggcc
     5761 ggaggcgccg ctcgccgccg gcatcgtcga c
//
LOCUS       DASYJA010000162         3714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_844254, whole genome shotgun sequence.
ACCESSION   DASYJA010000162 DASYJA010000000
VERSION     DASYJA010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844254"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VGO02_03420"
     CDS             complement(<1..448)
                     /locus_tag="VGO02_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV7476769.1"
                     /translation="MNPIQMVNPAAEYRLMQAELDAAVLRVLASGRYILGPEGEALEK
                     ELAAYLGAAHAVGCNSGTDALHLPLVAAGIGPGDEVVVPAFTFFATAEAVSYTGATPV
                     FADIDADTFNLSVKSLQKKITSKTKAVIAVLLFGQCAALDAISDTCK"
     gene            complement(445..1368)
                     /locus_tag="VGO02_03425"
     CDS             complement(445..1368)
                     /locus_tag="VGO02_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV7476770.1"
                     /translation="MGKVRVAVVGAGHMGRYHAQKFAGCEGAELAAVVDADASRAAAF
                     PGTPFFRDYRQMLDLADAAVIAVPTDRHYAIARACIAHGLHVLMEKPIAGTLAQATTL
                     IRLAKKHRVVLQVGHVERFNRGFAAISARLDRPMYIETERLAPFKARGVEVDVVLDLM
                     IHDLDLVVALARSEVKRVSASGFRVLTRDIDIATAYIEFASGCVANLSASRVSQAAVR
                     KLRMFQSGVYLSADLQSGAVRFATQSGGEVQQTEKNYSGGDALAAQAASFIKAISGKS
                     RVAVSGEDGRRALKLALDVARLVRVRLRRFE"
     gene            complement(1377..2237)
                     /locus_tag="VGO02_03430"
     CDS             complement(1377..2237)
                     /locus_tag="VGO02_03430"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HEV7476771.1"
                     /translation="MTRIALGFVWLLRLFPLKVISTVGSAVGAVMYWVIAERRNVIRV
                     NLAKCFPQMPAKEREQLARAHFRAFCRALFEHGVLWWSSRERIARLVRFEGLEHLRAL
                     QGKPVILFAPHFVGLDATLQRLSLEFPVAMMYARQKNPLMERVLYRGRTRFGGRMFPR
                     QAGVKEGLRAIAEGARYYYLPDLDYGRKRSIFVPFFGVSAATVTGLSYIARSSGAAVV
                     PCVTRMLPGGGYVARLWPAWSDFPGASDQADTRRMMAFIEERVLEMPEQYFWLHKRFK
                     TRPEGEAKFY"
     gene            complement(2234..3055)
                     /locus_tag="VGO02_03435"
     CDS             complement(2234..3055)
                     /locus_tag="VGO02_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV7476772.1"
                     /translation="MAAKLPLRVLHAVGTVLGWTVYGMSPTYRRHLRENLAQAGITDA
                     RTRRDTIAAVGQMVAELPALWFRPQHEVAALVKGVEGAAAEALAAAAQRRPLLFLAPH
                     MGSFEVTAQYASLQVPITVLYRAPKMAWLEPLMQEGRSRGRVRLAPADLGGVRELMAA
                     LKRGEAAGFLPDQVPGEGEGEWAEFFGRPAYTMTLAARLVEREGIEAFLAYARRLPRG
                     QGYVIVLRHLPEKLAGETATRRLNRAIESLVRECPGQYLWAYNRYKTPSGAKPRP"
     gene            3130..>3714
                     /locus_tag="VGO02_03440"
     CDS             3130..>3714
                     /locus_tag="VGO02_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HEV7476773.1"
                     /translation="MSEFLFTSESVSEGHPDKVADQISDAVLDAILAQDPRSRVAAET
                     LVKDNLVVLAGEVTTRAKVDYAAVARKTIKRIGYNDPALGFDAAGCTVQLAYGEQSPN
                     IAQGVDEGKGIDLDQGAGDQGLMFGYACDETPGLMPMAIYLSHRLVERQAEVRRDGRL
                     RWLRPDANSQVTLRYAAHGRPESIETVVLSTQHAP"
BASE COUNT          612 a         1291 c         1250 g          561 t
ORIGIN      
        1 ctttgcatgt gtctgaaatg gcatcgaggg ccgcgcactg gccgaacagg agtactgcaa
       61 tgacggcctt ggtcttcgaa gtgatctttt tctgcaggga tttgacggac agattgaagg
      121 tatccgcatc gatgtctgcg aaaaccggcg tggcgccggt gtacgagact gcctccgcgg
      181 tggcgaagaa ggtgaaggcc ggcaccacca cctcgtcccc cgggccgatg ccggcggcga
      241 ccagcggcag gtgcagcgcg tcggtgccgg agttgcagcc cacggcgtgc gcggcgccga
      301 ggtaggcggc gagctccttc tccagcgcct cgccctccgg accgaggatg tagcggccgg
      361 aggcgagcac gcgcagcacc gccgcatcga gctcggcctg catcaggcgg tactccgctg
      421 ccgggttgac catctggatt ggattcattc gaacctcctc aggcgcacgc gcaccaggcg
      481 cgcgacatcg agcgcgagct tgagcgcgcg gcggccgtct tcgccggaca cggcaacccg
      541 ggattttcct gaaattgcct tgataaaact cgccgcctgc gccgccagcg cgtcgccgcc
      601 ggaatagttc ttctccgtct gctgcacttc gccgccgctc tgggtagcga agcgcaccgc
      661 gccggactgc aggtcggccg acaggtagac gccgctttgg aacatgcgca gcttgcgcac
      721 cgccgcctgg ctgacgcggc tcgcggaaag gttggcgacg cagccgctcg cgaactcgat
      781 gtaagcggtg gcgatgtcga tgtcgcgcgt cagcacgcgg aagccgctcg cgctcacgcg
      841 cttcacctcc gagcgcgcca gggccactac caggtcgagg tcatggatca tcagatcgag
      901 caccacgtcc acctccacgc cgcgggcctt gaacggcgcc aggcgctcgg tctcgatata
      961 catcggccgg tcgagacgcg cgctaatcgc cgcgaagccg cggttgaagc gctcgacatg
     1021 accgacctgc agcaccaccc tgtgcttctt cgccaggcgg atcagcgtgg tggcctgcgc
     1081 cagggtcccg gcgatcggct tctccatgag gacgtgcagg ccgtgcgcaa tgcaggcccg
     1141 cgcgatggcg tagtgccggt cggtgggaac ggcgatcacg gcggcgtcgg cgaggtccag
     1201 catctgcctg tagtcgcgga agaacggcgt cccgggaaag gcggccgcgc gcgacgcatc
     1261 agcgtcgacc accgcggcga gctcggcgcc ctcgcagccg gcaaacttct gcgcgtggta
     1321 gcgccccatg tgccccgcgc cgaccacggc gaccctcacc ttgcccatgg tcttcttcaa
     1381 tagaatttcg cttcgccttc gggccgggtc ttgaagcgct tgtgcagcca gaagtactgc
     1441 tcgggcatct ccagcacgcg ttcctcgatg aaagccatca tgcggcgcgt gtccgcctgg
     1501 tcgctcgcgc ctggaaaatc gctccacgcc ggccacagcc tggcgacata gccgccgccc
     1561 ggaagcatgc gcgtcacgca gggcaccacg gccgccccgc tcgagcgagc aatatacgac
     1621 agcccggtca cggtggcggc gcttacgccg aaaaacggca cgaagatgga ccgcttgcgg
     1681 ccgtagtcga gatcgggaag gtagtagtag cgcgcgcctt cggcgatcgc gcgcagcccc
     1741 tctttcacgc ccgcctgccg cgggaacatg cggccgccga agcgcgtgcg gccccggtac
     1801 agcacgcgct ccatcagcgg attcttctgc cgcgcgtaca tcatggccac cgggaactcg
     1861 agcgacaggc gctgcagcgt cgcgtcgagg ccgacgaaat gcggcgcgaa caggatgacc
     1921 ggcttgccct gcagcgcgcg caggtgctcg agcccttcga agcgcaccag gcgcgcgatg
     1981 cgctcgcgcg acgaccacca cagcacgccg tgctcgaaca gcgcgcggca gaaggcgcgg
     2041 aaatgcgccc gcgcgagctg ctcccgctcc ttcgccggca tctgcggaaa gcacttcgcg
     2101 aggttgacgc ggatcacgtt gcgccgctcg gcgatcaccc agtacatgac ggcgccgaca
     2161 gcgcttccca ccgtcgaaat gaccttgagg ggaaacagcc gcagcagcca gacgaagccg
     2221 agcgcgatcc tggtcatggc cggggtttgg cgccgctcgg cgtcttgtag cggttgtagg
     2281 cccacaggta ctgccccggg cattcgcgca ccagcgactc gatggcgcgg ttgagccgcc
     2341 tggtcgcggt ttcacccgca agtttctccg gcaggtgccg gagcacgatc acgtagccct
     2401 ggccgcgcgg caggcgccgc gcgtaggcga ggaaggcctc gatcccttcg cgctcgacga
     2461 gccgggcggc gagcgtcatg gtgtaggccg gcctgccgaa gaactccgcc cattcgccct
     2521 cgccctcgcc gggcacctgg tcgggcagga agccggccgc ctcgccgcgc ttgagcgccg
     2581 ccatcagctc gcgcacgccg cccagatctg caggcgcaag gcggacccgg ccgcgcgagc
     2641 gcccttcctg catgagtggt tcgagccagg ccatcttcgg cgcgcggtag agcacggtga
     2701 tcggcacctg cagcgacgcg tactgcgccg tgacttcgaa cgagcccatg tgcggggcga
     2761 ggaacaggag cggccggcgt tgcgcggcgg cggcgagcgc ctcggcggcc gccccctcga
     2821 cgcctttcac cagcgcggcc acctcgtgct gcgggcgaaa ccacagcgcc ggcagctcgg
     2881 cgaccatctg cccgaccgcg gcgatcgtgt cgcgccgcgt gcgcgcatcg gtgatgccgg
     2941 cctgcgcgag gttctcgcgc aggtgcctgc ggtaggtggg ggacatccca tagaccgtcc
     3001 agccgaggac ggttcccacc gcgtgcaaca cgcggagcgg caatttcgca gccattcgga
     3061 ggagaaagcg agggaattcg tgcaggatta tttgatatcc ttgcggtctt ttcccgagac
     3121 ggtaaaacaa tgagcgaatt tctgtttact tccgagtccg tttccgaggg ccaccccgac
     3181 aaggtcgcgg accagatctc ggatgcggtg ctcgacgcga ttctagccca ggacccacgc
     3241 tcgcgcgtcg ccgccgagac gctggtgaag gacaacctgg tggtgctcgc cggcgaagtc
     3301 accacgcgcg ccaaggtcga ctatgccgcg gttgcgcgca agacgatcaa gcgcatcggc
     3361 tacaacgatc cggcgctcgg cttcgacgcc gccggctgca ccgtgcaact cgcctatggc
     3421 gagcagtcgc ccaacatcgc gcagggcgtg gacgaaggca agggcatcga cctcgaccag
     3481 ggcgcgggcg accagggcct gatgttcggc tacgcctgcg acgagacgcc gggcctgatg
     3541 ccgatggcga tctacctctc gcaccgcctg gtcgagcgcc aggccgaggt gcgtcgcgac
     3601 gggcgcctgc gctggctgcg tcccgacgcg aactcccagg tcacgctgcg ctacgccgcg
     3661 cacggcaggc ccgagtcgat cgagaccgtg gtgctctcga cgcagcacgc gccg
//
LOCUS       DASYJA010000163         5231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_752976, whole genome shotgun sequence.
ACCESSION   DASYJA010000163 DASYJA010000000
VERSION     DASYJA010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752976"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..751)
                     /gene="kdsB"
                     /locus_tag="VGO02_03445"
     CDS             complement(32..751)
                     /gene="kdsB"
                     /locus_tag="VGO02_03445"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEV7476774.1"
                     /translation="MNFHVVIPARYASTRFPGKPLADLAGKPMVVRVCERAAKSGASA
                     VHVATDDERIASAVREHGHSALMTRADHASGTDRLAEAAAQLKLKDDQIVVNLQGDEP
                     LMAPALIGQVAALLAKKQDAVVSTACHAIHDALDNPNIVKVVLDHEGYALYFSRSRIP
                     FPREPGGVCYRHAGIYGYRVGFLKKFSSLEQSPLERAEALEQLRVLWHGFRIAVAVSE
                     SEIPPGVDTPQDLEAVRKMFS"
     gene            complement(748..948)
                     /locus_tag="VGO02_03450"
     CDS             complement(748..948)
                     /locus_tag="VGO02_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEV7476775.1"
                     /translation="MSGVDPRLLEILVCPLCKGKLVYKKEAKAGPELICKADRLGYPV
                     KDGIPVMLEEEARKLPPEEEVV"
     gene            complement(920..1897)
                     /gene="lpxK"
                     /locus_tag="VGO02_03455"
     CDS             complement(920..1897)
                     /gene="lpxK"
                     /locus_tag="VGO02_03455"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEV7476776.1"
                     /translation="MRFWYRRGAIAWLLWPVSLLFGVLVVLRRVLYFIRVLKSRRAGI
                     PVIIVGNITVGGSGKTPLVIWIAEFLKARGWSPAIVSRGYGAKVQTPRAATIASEAAE
                     VGDEPVLLSRRSGCPVWVGADRLAVIAALRAANPDVDVLILDDGLQHYRLRRDLEIAV
                     VDGRGLGNGFLLPAGPLREPAWRLRTVDAVVVNGASKKQDFTMLLEGDTLHRMSDARE
                     RQPLKAFAGRKVHAVAGIGDPKRFFLQLGAAGIKVQPHPFPDHHAFTPHDLEFGDELP
                     VLMTEKDAVKLRHAAQPGWWVLPVTARLDAAFGNWLLRKCDEWRRSKAA"
     gene            complement(1897..2313)
                     /locus_tag="VGO02_03460"
     CDS             complement(1897..2313)
                     /locus_tag="VGO02_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEV7476777.1"
                     /translation="MNFQRGKEKEPLEINLVPMIDVMLVILIFLMITTTYSKYTELQI
                     NLPTANAEKQLERPNEVTVLVNAQGQYVVNRAPVAFRSIEQLADELRRAAAAMKEPVV
                     VISADATATHQAVIRVMEASRLAGLSQITFTTQSAK"
     gene            complement(2315..2944)
                     /locus_tag="VGO02_03465"
     CDS             complement(2315..2944)
                     /locus_tag="VGO02_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEV7476778.1"
                     /translation="MFAIIQAAGWPIWPLLLASVIALALIIERFVSLREPKIIPPLLL
                     EEAIGVYKRQGVDEDMLARLAKDSPLGTVLAAGLRNHRSSRYVMKEAIDEAGRHVAHD
                     LERFLTTLGTIATAAPLLGLFGTVIGMIEIFGSQGPTGTNPQQLAHGISVALYNTAFG
                     IAIAIPALIFYRHFRNKVDTLVVEMEQLAAKLIDIVHGERMDFQPQQRA"
     gene            3461..4654
                     /gene="xseA"
                     /locus_tag="VGO02_03470"
     CDS             3461..4654
                     /gene="xseA"
                     /locus_tag="VGO02_03470"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEV7476779.1"
                     /translation="MESTILTVSELLRSVRDTLERRFPLLWVRGELSNLSRAPSGHWY
                     FTLKDGGAQVDCVMFRSRAAALDWEPRDGMQVEARALVTLYEPRGRFQLTVEGLRRGG
                     VGPLFERFVRLKEKLEREGLFEPGLKRELPAHPRAIGVLTSRAAAALRDVLTTLARRN
                     PAVPVIVYPIPVQGAGAAERIAAMLSRAGRRAECDVLLLVRGGGSIEDLWEFNEEALA
                     RAIRACPIPVVVGVGHETDFTIADFAADRRAPTPTAAAELASPSRAELLGRVAECARC
                     VSREMRRRLDYAAQAADGWVRRLLHPAERLRSYQGLVGQLSARLGFAFLQRLKDCAAM
                     LAGLQGSLRSLDPAAVLERGYSITSNAQGEVLRDAALLREGERVHTRLARGEFESEVK
                     KRENS"
     gene            4659..5213
                     /locus_tag="VGO02_03475"
     CDS             4659..5213
                     /locus_tag="VGO02_03475"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEV7476780.1"
                     /translation="MAHRLSKITTRTGDTGETGLGDGARVSKDSARIHALGDIDELNS
                     ALGLVLAEEVPAALREALGEVQHDLFDLGGELSIPGHALLRAEQVERLERQLEAWNAE
                     LPALKEFILPGGSRAAAAAHLARTICRRAERSVVTLGRAETVSEQVRRYLNRLSDLLF
                     VAGRVLNRHAGRGDVQWRHQRKKP"
BASE COUNT          820 a         1737 c         1793 g          881 t
ORIGIN      
        1 cctgctccgg ggggtcccag aagaatcagg ttcatgaaaa cattttacgc acggcttcca
       61 gatcctgagg cgtgtcgacc cccggcggga tctcgctttc gctgaccgct accgcaatgc
      121 ggaaaccgtg ccagagaacc cgcagctgct cgagggcctc ggccctttcg agcgggctct
      181 gctccaggga tgaaaacttc ttcaggaagc cgacccggta gccgtagata ccggcatgac
      241 ggtagcagac gcctcccggc tcgcgcggga aaggaatccg cgagcgagag aaatagaggg
      301 cgtagccctc gtggtcgagc actaccttca cgatgtttgg attgtccagg gcgtcgtgga
      361 tggcatggca ggcggtcgag accacggcgt cctgcttctt cgccagcagc gcggccacct
      421 ggccgatcag cgcgggcgcc atcagcggct cgtcgccctg caggttcacc acgatctggt
      481 cgtccttcag cttgagctgc gccgccgctt ccgccagccg gtcggtgccc gaggcatggt
      541 cggcgcgggt catcagcgcg gagtggccgt gctcgcgcac cgcgcttgcg atgcgctcgt
      601 cgtcggtcgc gacatgcacg gccgacgctc cgcttttcgc tgcgcgctcg cacacgcgca
      661 ccaccatcgg cttgccggcg aggtcggcga gcggctttcc gggaaagcgg gtcgacgcgt
      721 agcgcgccgg gatgactaca tgaaaattca cactacttct tcttcgggag gcagcttgcg
      781 ggcttcttcc tccagcatca cggggatgcc gtccttcacc gggtagccga ggcggtcggc
      841 cttgcagatc agctcgggcc cggccttggc ttctttcttg tagacgagct tgcccttgca
      901 caacgggcag accaggattt caagcagcct tggatctacg ccactcatcg catttcctca
      961 gcagccagtt gccgaacgcg gcgtcgagcc gcgcggtcac cggcagcacc caccatcctg
     1021 gctgcgcggc gtggcgcaat tttaccgcgt ccttttcggt catcagcacc ggcagctcat
     1081 cgccgaactc gaggtcatgc ggcgtgaacg cgtgatggtc ggggaaggga tgcggctgca
     1141 ccttgatgcc cgcggcgccg agctgcagga agaagcgctt cgggtcgccg atgccggcga
     1201 ccgcgtgcac cttcctgccg gcgaacgcct tgagcggctg ccgctcgcgc gcgtcgctca
     1261 tgcggtgcag cgtgtcgcct tcgaggagca tggtgaagtc ctgcttcttc gaggcgccgt
     1321 ttaccacgac cgcgtccacg gtgcgcagcc gccacgccgg ctcgcgcagc ggccccgccg
     1381 gcagcaggaa gccgttgccg aggccgcggc cgtcgaccac cgcgatttcg aggtcgcggc
     1441 gcagccggta gtgctgcagg ccatcgtcga gaatcagcac atccacgtcg ggattcgcgg
     1501 cgcgcagcgc ggcgatgacc gccaggcggt cggcgccgac ccacaccggg cagccgctgc
     1561 ggcgcgaaag caggaccggc tcgtcgccga cctccgctgc ctcggaggcg atggtcgcgg
     1621 cacgcggcgt ctgcaccttg gcgccgtagc cgcgcgagac gatcgccggc gaccagcccc
     1681 ttgctttcag gaactctgcg atccagatca ccagcggcgt cttgccgctg ccgcccaccg
     1741 tgatgttgcc gacgatgatt acggggattc cggcgcgtct gctttttaga acacgaatga
     1801 aatagagaac gcgcctgaga accaccagga ctccaaacag gaggctgacc ggccacagca
     1861 gccaggcgat ggcgccgcgg cggtaccaga agcgcattac ttcgcggact gcgttgtaaa
     1921 agtgatctgc gacaggccgg cgaggcgcga ggcctccatg acgcgtatta cggcctggtg
     1981 cgtcgccgtg gcgtcggcgc tgatcaccac caccggctcc ttcatggcgg cggccgcgcg
     2041 cctgagctcg tcggcgagct gctcgatcga gcggaaggcc accggcgcgc ggttgacgac
     2101 gtactgcccc tgggcgttga ccagcacggt gacttcgttc gggcgctcga gctgcttctc
     2161 ggcgttggcg gtcggcaggt tgatctgcag ctcggtgtac ttcgagtagg tggtggtgat
     2221 catgaggaag atcaggatca ccagcatgac gtcgatcatc ggcacgaggt tgatctcgag
     2281 cggctccttc tccttgccgc gctggaagtt catgctaggc gcgctgctgc ggctggaagt
     2341 ccatgcgctc gccgtgcacg atgtcgatca gcttggcggc gagctgctcc atctccacca
     2401 ccagcgtgtc gaccttattc ctgaagtggc ggtagaagat gagcgccggg atcgcgatgg
     2461 cgatgccgaa ggcggtgttg tagagcgcga ccgagatgcc gtgcgcgagc tgctgcgggt
     2521 tggtgccggt aggcccctgc gagccgaata tctcgatcat gccgatcacc gtgccgaaca
     2581 ggccgagcag cggcgcggcg gtggcgatgg tgccgagcgt ggtgaggaag cgctccaggt
     2641 cgtgtgccac gtggcgcccc gcctcgtcga tcgcctcctt catcacgtag cgcgagctgc
     2701 gatggttgcg caggccggcg gcgagcaccg tgcccagcgg cgagtccttg gccagcctgg
     2761 ccagcatgtc ctcgtcgacg ccctggcgct tgtacacccc gatcgcttcc tcgagcagca
     2821 gcggcggaat gatcttgggc tcgcgcagcg acacgaagcg ctcgatgatc agggccagcg
     2881 cgatcacgga ggcaagcagc aggggccaga tcggccagcc tgcggcttga ataatggcga
     2941 acaactcgtt ttgccttgtg tttttttggg ttgggtcgcc ggcgacttta agccaaaccg
     3001 ccgtgcggcg ccaatttctg cagtgcaaca accggccgcg cgttgccgcc ggagcgcggc
     3061 accatgtagt gcaatccgcg atatccacaa aaatctgtgg ataagtttgt ggatagaagc
     3121 ggtatagagc ttcccaagcc agttgcagcg cccgccgaga cggtggcgat caaattttga
     3181 gcagcttttt cccgctggca aacagggact tagcggtaag aatcggcgtc cgcgcgcggt
     3241 ccagtcgccg tcgggcgtcg cggcgccgcc cgtgtggatt aatgggtatg tctcccttga
     3301 cgccaagtga gcgattgcgg ggcgtcagct acaggaatgc gaggctgttt tgggtgctgt
     3361 agagcggctt cccggagggc ttaaatgggg gtcgtgctta aattggaact gggtggagga
     3421 ttcgggagtt caaccgacgc tcttcggccc gcaccgtccc ttggaaagca ccatcctcac
     3481 cgtttccgag ctccttcgca gcgtccgcga cacgctggag aggcgctttc ccctgctctg
     3541 ggtgcgcggg gagctgtcca acctgtcgcg cgccccgtcc ggccactggt atttcaccct
     3601 caaggacggc ggcgcgcagg tcgactgcgt gatgttccgc agccgtgccg cggcgctcga
     3661 ttgggagccg cgcgacggca tgcaggtcga ggcgcgcgcg ctggtcaccc tgtacgagcc
     3721 gcgcggccgc ttccagctca ccgtcgaagg cctgcgccgc ggcggcgtcg gaccgctgtt
     3781 cgagcgcttc gtgcgtctga aagagaagct cgagcgcgag ggcctgttcg agccaggctt
     3841 gaaacgcgag ctgccggcgc atccgcgggc gatcggcgtg ctcacctcgc gcgccgccgc
     3901 cgcgctgcgc gatgtgctca cgacgctggc gcggcgcaac ccggcagtcc cggtgatcgt
     3961 ctacccgatc cccgtgcagg gcgcgggcgc cgccgagcgc atcgccgcga tgctgtcgcg
     4021 cgccggcagg cgcgccgagt gcgacgtgct gctcctggtg cgcggcggcg gctcgatcga
     4081 ggacctgtgg gaattcaacg aggaggcgct ggcacgggcg atccgcgcct gtcccatccc
     4141 ggtggtagtc ggcgtcggcc atgaaacgga cttcaccatc gccgatttcg ccgccgaccg
     4201 gcgcgcgccg acgccgaccg ccgcggcgga gctggcgagc ccttcgcgcg ccgagctgct
     4261 cggccgcgtc gcggagtgcg cgcgctgcgt ctcgcgcgag atgcgccggc ggctcgacta
     4321 cgcggcgcag gctgccgacg gctgggtgcg ccgcctgctg catccagccg agcgccttcg
     4381 ctcgtatcag ggtctggtgg gccagctctc ggcaaggctc ggctttgcct tcctccagcg
     4441 cctgaaggac tgcgcggcga tgctggcggg cctgcagggc tcgctgcgct cgctcgaccc
     4501 ggcggcggtg ctggagcgcg gctacagcat caccagcaat gcacaaggtg aggtgctgcg
     4561 cgacgcggcg ttgctgcgcg aaggcgagcg ggtgcacacc cgcctggccc gcggcgagtt
     4621 cgaaagtgaa gtgaaaaaaa gagaaaattc ataatcccat ggcgcaccga ctgtcgaaaa
     4681 tcaccacccg caccggcgat accggcgaga ccggcctggg cgacggcgcg cgcgtgtcga
     4741 aggacagcgc ccgcatccac gcgctgggcg acatcgacga gctgaactcg gcgctcggcc
     4801 tggtgctcgc cgaggaagtg cccgccgccc tgcgtgaagc gctaggcgag gtgcagcacg
     4861 atttgttcga cctcggcggc gagctcagca tcccgggcca cgcgctgctg cgcgcggagc
     4921 aggtcgagcg cctcgagcgc cagctcgagg cctggaacgc cgagctgccg gcgctgaagg
     4981 aattcatcct gcccggcggc tcgcgcgccg cggctgccgc gcacctcgcg cgcaccatct
     5041 gccgcagggc cgagcgcagc gtggtgaccc tgggacgggc cgaaacggtc agcgagcagg
     5101 ttcgccggta cctcaaccgc ctctcggacc tgctgttcgt cgccgggcgg gtgctcaacc
     5161 gccatgccgg ccgcggcgac gtgcagtggc gccaccaacg caagaaaccg tgagcgtccc
     5221 cgaccccctg g
//
LOCUS       DASYJA010000164         3997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3204516, whole genome shotgun sequence.
ACCESSION   DASYJA010000164 DASYJA010000000
VERSION     DASYJA010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3204516"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VGO02_03480"
     CDS             complement(<1..438)
                     /locus_tag="VGO02_03480"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEV7476781.1"
                     /translation="MRAMRRVTLTQFLVARQRDKNLINADLRLLLETIARACKAISTR
                     VSKGALADGHGSAGTSNIQGEVQKKLDVLSNEILLEANEWGGNLAALASEEMDDPRPI
                     PTHYPRGEYLLLFDPLDGSSNIDVNISVGTIFSILRCPKLDSGG"
     gene            475..1422
                     /locus_tag="VGO02_03485"
     CDS             475..1422
                     /locus_tag="VGO02_03485"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEV7476782.1"
                     /translation="MTKLVAALLVSLLSFTAAFANDDGDRTEDLLKAVRKARLIDLSH
                     TWDKFSPIASVNPPYSFALAATHANTRGTFGDGGQLSFTSEVMHWSNQHGAPSIDAIG
                     HIGRDGKLFGGVDAAASTTNPDGIGTSGTGAHLAIDRFPNDLLINRGILLDVATMIQG
                     NSSPLPAQFEITAAHLEQAAKRQKVTLKKGDTVFIRTGWGPLFKSDPAMYAGAFSPGP
                     SLGGAEFLIKHGARVVGDDTLTFEQRPPIVLEPKFQVFPVHMRLIADSGIYIIENLNL
                     EELAKAKAYEFAVVVPPIKILGGTGSAVRIFALVPRDDD"
     gene            complement(1378..2550)
                     /locus_tag="VGO02_03490"
     CDS             complement(1378..2550)
                     /locus_tag="VGO02_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7476783.1"
                     /translation="MEIVYFVTLNSLGHLAFVGSRMASALFALQLGASPVEVGVVMAL
                     FAALPMLLSVSTGRLIDRSGPRRPLLVALAALACGDALPFFFPRLEVLYLSSTILGVG
                     FMFVHIGMNSVIGAYGKPQDRPMNFSWLALSFSISGSAGPLIAGFAIEGLGYADAFIA
                     LGIFPLIALALLWLRKQPLPRPLRPHAAGKHHLLDLLRVPGLRRTFLVSGMMAMGWDL
                     YSFLMPLYSARLGLGAVTIGFIMATFAVATFVVRLAMPVLIRRVRQWRIITASLTIAG
                     TSYLLFPLVASAPLLMTISFVLGLGLGCAQPVIMSLLYEAAPEGRQGEAVGIRTTMIN
                     ASQTLIPLGSGALSLALGMTPVFWLLAGGLLGAAWFARRQARSVVVARHQGEDADR"
     gene            complement(2523..3404)
                     /locus_tag="VGO02_03495"
     CDS             complement(2523..3404)
                     /locus_tag="VGO02_03495"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV7476784.1"
                     /translation="MNALVAWYFVAVWGAGYVATKAGLQYAPPFTLLSLRFLFGLLCL
                     VPVLFFFRARWPGTRAGWGHLAAAGLLMHVVQLGGSHYAQYLGMSAGVAALVISCQPL
                     ATAVIAVRFMGEKLAPRQWLGVFLGLAGVALVVWHKIDIREVTAASLVATLIALTGVT
                     AGTLYQRVFSPNCDLKAAAAIQFAAAALAFVPLAIAVEGFQVRWSATLAASVVFLVIF
                     TSLLGVSALYWLTRHGEASRVTSMMYLPAVFAVVLELALFGVAPGALATAGIALTCLG
                     VALTVWKPKWKSSTSSR"
     gene            complement(3617..>3997)
                     /locus_tag="VGO02_03500"
     CDS             complement(3617..>3997)
                     /locus_tag="VGO02_03500"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEV7476785.1"
                     /translation="LNTFVFFLSRVVGMIVDKVVFRTERGIGPGFYITVIVSQILFGI
                     LASLIVAWFSRYREFRADAGSAHLLGSPKPMVAALARLGGLDSGGLPDQVKAFGINSN
                     SGLFHLFATHPPIEARIAALQAGA"
BASE COUNT          725 a         1336 c         1396 g          540 t
ORIGIN      
        1 gccgccgctg tcgagcttgg ggcagcgcag gatggagaag atggtgccca ccgagatgtt
       61 gacgtcgatg ttggacgagc cgtccagcgg gtcgaacagg agcagatatt ccccgcgcgg
      121 gtagtgcgtc gggatggggc gcgggtcgtc catctcctcg gaggcgaggg cggcgaggtt
      181 gccgccccac tcgttggcct cgagcaggat ctcgttggac agcacgtcga gcttcttctg
      241 cacctccccc tggatgttcg aggtgccggc cgagccgtgg ccgtcggcca gggcgccctt
      301 gctgacgcgg gtgctgatgg ccttgcaggc gcgggcgatg gtttccagca gcaggcgcag
      361 gtcggcgttg atcaggttct tatcgcgctg tcgggcgacc aggaattgcg taagagtgac
      421 gcgtcgcatg gcgcgcattc taaggggtat gatcagcccc agggaggtgg acccatgacc
      481 aagctcgtcg cagcattgct cgtctcgctt ctgtccttca ctgcagcatt cgccaacgat
      541 gacggcgacc gcaccgagga tctcctcaag gcggtcagga aggcgcgcct catcgacctg
      601 tcccacacct gggacaagtt ctcgccgatc gcctcggtga acccgccgta ctcgttcgcg
      661 ctcgccgcca cgcacgccaa cacgcgcggc accttcggcg acggcggcca gctctccttc
      721 acttccgagg tcatgcactg gagcaaccag cacggcgcgc cgagcatcga cgccatcggc
      781 catatcggcc gcgacggcaa gctgtttggc ggagtcgacg cggcggcctc gaccaccaac
      841 ccggacggca tcggcacgag tggtaccggc gcgcacctcg cgatcgaccg cttcccgaac
      901 gacctgctga tcaaccgcgg catcctgctg gacgtcgcca cgatgatcca gggcaactcg
      961 agcccgttgc cggcccagtt cgagatcacc gccgcccacc tcgagcaggc ggccaagcgc
     1021 cagaaggtga cgctgaagaa gggcgacacg gttttcatcc gcaccgggtg ggggccgttg
     1081 ttcaagtccg acccggcgat gtacgccggc gctttctcgc ccggtcccag cctgggcggc
     1141 gcggagttcc tgatcaagca cggcgcgcgc gtggtcggcg acgacaccct gaccttcgag
     1201 cagcggccgc cgatcgtgct cgagccgaag ttccaggtgt tcccggtgca catgcgcctg
     1261 atcgccgaca gtggcatcta catcatcgag aacctcaacc tggaagagct ggcgaaggcg
     1321 aaggcctacg agttcgcggt ggtcgtgccg ccgatcaaga tactcggcgg caccggctca
     1381 gcggtccgca tcttcgccct ggtgccgcgc gacgacgact gagcgcgcct ggcggcgcgc
     1441 gaaccacgcc gcgccgagca ggccgccggc gagcagccag aacaccggcg tcatgccgag
     1501 cgcgagcgac agcgcgcccg agccgagcgg gatcagggtc tggctggcgt tgatcatcgt
     1561 ggtgcggatg ccgaccgcct cgccctggcg cccctccggc gcggcctcgt agagcagcga
     1621 catgatcacc ggctgcgcgc atcccaggcc gaggccgagc acgaacgaga tcgtcatcag
     1681 caggggggcg ctcgccacca gcgggaacag caggtagctg gttcccgcga tggtcagcga
     1741 ggcggtgatg atgcgccatt gccgcacgcg ccggatgagc accggcatcg ccagcctgac
     1801 gacgaaggtc gccacggcga aggtcgccat gatgaagccg atggtcaccg cgccgagccc
     1861 gagccgcgcg ctgtagagcg gcatcaggaa cgagtagagg tcccagccca tcgccatcat
     1921 gccgctcacc aggaaggtgc ggcgcaggcc cggcacgcgc agcaggtcga gcaggtggtg
     1981 cttgcccgcg gcatgcggcc tgagcgggcg cggcagcggc tgcttgcgca gccagagcag
     2041 cgccagggcg atcagcggaa aaatccccag cgcgatgaag gcgtccgcgt agcccagccc
     2101 ctcgatggcg aagccggcga tcagcgggcc ggccgagccc gagatggaga agctcagcgc
     2161 cagccaggag aaattcatcg gccggtcctg cggcttgccg taggcgccaa tgacgctgtt
     2221 catgccgatg tgcacgaaca tgaagccgac gccgaggatg gtgctcgaca ggtagagcac
     2281 ctccaggcgc gggaagaaga agggcagcgc gtcgccgcag gcgagcgcgg cgagggcgac
     2341 gagcagcggc cggcgcggcc cgctgcggtc gatcaggcgc ccggtgctca ccgagagcag
     2401 catcggcaac gccgcgaaca gcgccatcac cacccccacc tcgaccggcg aggcgcccag
     2461 ctgcagcgcg aacagggcgc tcgccatccg gctgccgacg aaggccaggt ggccgaggga
     2521 attcagcgtg acgaagtaga cgatttccac tttggcttcc acaccgtcag cgccacgccc
     2581 aggcaggtga gggcgatgcc ggccgtggcc agcgcgcccg gcgcgacgcc gaacagcgcc
     2641 agctcgagca cgacggcgaa gaccgccgga agatacatca tgctcgtgac gcggctcgct
     2701 tcgccgtggc gcgtcagcca gtacagcgcg ctgacgccga gcagcgaggt gaagatcacc
     2761 aggaacacca ccgaagcggc gagcgtcgcg ctccacctga cctggaaccc ctcgacggcg
     2821 atggcgagcg gcacgaaggc aagggcggcg gcggcaaact gaatcgccgc ggccgctttc
     2881 aagtcgcaat tcgggctgaa cacccgctgg taaagcgtgc cggccgtgac gccggtgagc
     2941 gcgatcaggg tcgccaccag gctggcggcg gtgacctcgc gaatgtcgat cttgtgccag
     3001 accacgagcg ccacgccggc gagcccgagg aacactccga gccactggcg cggcgccagc
     3061 ttctcgccca tgaagcgcac cgcgatgacg gccgtggcga gtggctggca ggagatcacc
     3121 agggcggcga cgcccgccga catgccgagg tattgcgcat agtggctgcc gccgagctgc
     3181 accacgtgca tcagcaggcc ggcggcggcg agatggcccc atccggcgcg cgtgcccggc
     3241 cagcgcgccc tgaagaaaaa cagcaccggg accaggcaca gcaggccgaa gaggaagcgc
     3301 aggctgagga gggtgaacgg cggcgcgtac tgcagtccgg ccttggtggc gacatagccg
     3361 gcgccccaga ccgctacgaa gtaccaggcg acgagggcgt tcatccgcag cggcggattc
     3421 taatccagcg gactttttcc tcgttgctac aaacgggcaa caagcgggat atagcggcga
     3481 tacccgtcgc cgctaatctg ccggatatga aaaagctgac ctactggacg ttcgcgccgc
     3541 tggcgcgctt ttgcggaaag ctggtttcga tccgcggcgc cgggttgcag cctgcgccgt
     3601 ccatgcagga cgctgcctag gcgcccgcct gcagcgccgc gatgcgcgcc tcgatcggcg
     3661 ggtgcgtggc gaagaggtgg aacaagcccg agttgctgtt gatgccgaag gccttgacct
     3721 ggtcgggcag gccgccgctg tcgaggccgc cgaggcgcgc cagcgcagcc accatcggct
     3781 tcggcgagcc gagcaggtgc gccgagccgg cgtcggcgcg gaactcgcgg tagcgcgaga
     3841 accaggcgac gatgagcgag gccaggatgc cgaacaggat ctggctgacg atgacggtga
     3901 tgtagaaccc cggcccgatg ccgcgctcgg tgcggaacac caccttgtcg acgatcatgc
     3961 cgacgacgcg cgacaggaag aagacgaagg tgttgag
//
LOCUS       DASYJA010000165         1831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2749479, whole genome shotgun sequence.
ACCESSION   DASYJA010000165 DASYJA010000000
VERSION     DASYJA010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2749479"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="VGO02_03505"
     CDS             <1..899
                     /locus_tag="VGO02_03505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7476786.1"
                     /translation="GVQGAPYDAVAVEEWFPKEGLERNAQPKGGFHPLLEDAARGLSI
                     RLASPVQRIAWSASGVSAILQNGEAIEAERALITVPVGLLRAGLPALDPPPPDDQRTA
                     IGRLGYGAGVLGKIYLRFPWRFWPEQPKWFGRLPDAPEKRGTFNTWVSHEQETGRPIL
                     LSFSNGATAARLDRTASDTEVKDVAMASLRKMFGADIPEPEAMLFPRWLSDPWSRGGY
                     SYPGVGSAPEDRPIHARPLGGRVYFAGEATEPVEYGTVHAALWSGEQAAEAIFRAATG
                     AAATRDARPWAGARAGAGRHNE"
     gene            924..1139
                     /locus_tag="VGO02_03510"
     CDS             924..1139
                     /locus_tag="VGO02_03510"
                     /inference="COORDINATES: protein motif:HMM:NF033233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HEV7476787.1"
                     /translation="MLAKILFVLVIISGLATLGMLFAGLISMSRNVDGNIEGGRRSNK
                     LMWWRVRLQFLTIILILLWYFANQSQG"
     gene            1144..1737
                     /locus_tag="VGO02_03515"
     CDS             1144..1737
                     /locus_tag="VGO02_03515"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEV7476788.1"
                     /translation="MVTLTRIYTRGGDAGQTSLGRGERVAKHDPRVEAYGTTDEANSV
                     IGLARSAIARTVLGGERYAEADAMLGRIQNDLFDLGADLCTPEGKSKRGEQALRIVAS
                     QVERLEKEIDAMNADLQPLNSFILPGGSEAAAWLHLARTVSRRAERRMTRLAAEQEIN
                     PEAIKYINRLSDHLFVLARRLNDDGATDVLWVPGGAR"
BASE COUNT          311 a          622 c          612 g          286 t
ORIGIN      
        1 aaggcgtgca gggggcgccc tatgacgccg tcgccgtcga ggaatggttc ccgaaagaag
       61 gcctcgaacg caacgcccag cccaagggcg gctttcatcc gctcctcgag gatgccgcgc
      121 gcgggctgtc gattcgactg gcctcgcccg tgcagcgcat cgcctggagc gcgtcgggcg
      181 taagcgcgat cctgcagaat ggcgaggcca tcgaagccga gcgggctttg atcaccgtgc
      241 cggtcggcct gctgcgcgcc gggctgccgg cgctcgatcc gccgccgccc gatgaccagc
      301 gcacggcgat cggccggctg ggctacggcg cgggggtgct gggcaagatc tacctgcgct
      361 ttccgtggcg cttctggccc gagcaaccga aatggttcgg ccgcctgccc gacgcgccgg
      421 agaagcgcgg caccttcaac acctgggtca gccacgagca ggagaccggc cggccgatcc
      481 tgctcagttt cagcaacggt gcgacggccg cgcgccttga ccggacggcc agcgataccg
      541 aagtgaagga cgtggcgatg gcctcgctgc gcaagatgtt cggtgccgac attcccgagc
      601 cggaagcgat gctctttccg cgctggctga gcgatccgtg gtcgcgcggc ggctattcct
      661 atcccggtgt cggcagcgcg ccggaggacc ggccgatcca cgcccgtccg ctcggcgggc
      721 gcgtctactt cgccggcgag gcgaccgagc cggtcgaata cggcacggtg catgccgcgc
      781 tgtggtcggg cgagcaggcg gccgaggcga tctttcgagc cgcgaccggc gccgctgcca
      841 cgcgagacgc gcgaccctgg gcaggcgcac gtgctggcgc gggccggcac aatgaatgat
      901 agaacacggc aaaggagggt gtgatgctcg ccaagatcct gttcgttctg gtgattatct
      961 ccggcctcgc cacgctcggc atgctgttcg ccgggttgat cagcatgagc cgcaacgtcg
     1021 acggcaacat cgaaggcggc cggcgcagca acaagctgat gtggtggcgc gtgcgcctgc
     1081 agtttctcac gatcatcctg atccttcttt ggtacttcgc caaccagagc cagggctgaa
     1141 ccgatggtga cactcacccg catctacacg cgtggcggcg acgccggcca gacctcgctc
     1201 ggccgcggcg agcgcgtcgc caagcacgat ccgcgcgtcg aagcctacgg cacgaccgac
     1261 gaagccaatt cggtgatcgg cctggcgcgc agcgccatcg cccgcacggt gctgggtggc
     1321 gagcgctacg ccgaagccga cgccatgctc ggccgcatcc agaacgacct gttcgatctc
     1381 ggcgccgatc tctgcacgcc cgaaggaaag agcaagcgcg gcgaacaggc gctgcgcatc
     1441 gtcgcttcgc aggtcgagcg gttggagaaa gagatcgacg ccatgaacgc cgaccttcag
     1501 ccgctcaatt ccttcatcct gcccggcggc agcgaggcgg ctgcatggct ccatcttgcc
     1561 cgcaccgttt cgcgccgcgc cgaacggcgc atgacccggt tggccgccga gcaggagatc
     1621 aaccccgagg cgatcaagta catcaaccgc ctctccgatc acctgttcgt gctcgcacgg
     1681 cggctgaacg acgacggcgc caccgacgtc ctgtgggtgc cgggtggcgc gcgctaggac
     1741 cttcaacgtg ttgaaaaatt gaggaaaaag cttccaccct gcgattttgt gcagtgcgcc
     1801 atagccttga catgatcggc tgaacacccc t
//
LOCUS       DASYJA010000166         3450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_207775, whole genome shotgun sequence.
ACCESSION   DASYJA010000166 DASYJA010000000
VERSION     DASYJA010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207775"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /gene="rfbC"
                     /locus_tag="VGO02_03520"
     CDS             complement(<1..433)
                     /gene="rfbC"
                     /locus_tag="VGO02_03520"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEV7476789.1"
                     /translation="MKVTPTSVPEVLILEPRVFEDPRGFFFESYNRRVFKEATGLDPQ
                     FVQDNQSFSVRNVLRGLHYQVRQPQGKLITVLAGEIFDVAVDLRRSSPTFGKWVSATL
                     SGGTHRAIWIPPGFGHGFLVLSEHALVQYKATDYYAPEHERT"
     gene            complement(433..1308)
                     /gene="rfbA"
                     /locus_tag="VGO02_03525"
     CDS             complement(433..1308)
                     /gene="rfbA"
                     /locus_tag="VGO02_03525"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEV7476790.1"
                     /translation="MKGIILAGGSGTRLYPVTQVVSKQLLPIYDKPMVYYPLSTLMLA
                     GIRDLLIISTPQDTPRFEELLGDGKRWGIRLRYAVQPKPEGIAQAFILGRKFIGKDPV
                     ALILGDNLFHGHDLTALLNKASQKKRGATIFAYPVDDPERYGVVQFDPTGKAIGIEEK
                     PKRPRSRYAVVGLYFYDNRVVDIAANLTPSARGELEITDVNKAYLERGQLDVMPMGRG
                     YAWLDTGTHGSLLEAAHFIETIERRQGLKIACPEEIAYRMGYISGAKLKALAQPLANS
                     GYGKYLLKVLAERVF"
     gene            complement(1305..2300)
                     /gene="rfbB"
                     /locus_tag="VGO02_03530"
     CDS             complement(1305..2300)
                     /gene="rfbB"
                     /locus_tag="VGO02_03530"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEV7476791.1"
                     /translation="MLLVTGGAGFIGSNFVLSTLAATGEAVVNLDKLTYAGNLRNLEA
                     LRGDARYQFVQGDICDRPLVRSLLEAHQPRAIVHFAAESHVDRSISGPAEFVQTNVVG
                     TFALLEEFRSYWEKESKSALRFLHVSTDEVYGSLGPTDPAFTEENPHAPNSPYAASKA
                     AADHMVRAYHHTYGLPTLTTNCSNNYGPHQFPEKLIPLIIHNALAGKALPVYGDGRNV
                     RDWLYVLDHCDAIRAVLERGRIGETYNIGGNSEKANIDLVRLLCSLLDQAAPRNSGRY
                     ADLISFVKDRPGHDRRYAIDSRKIQRELGWQPKESFESGLNKTIAWYVEATRRAA"
     gene            complement(2312..2692)
                     /locus_tag="VGO02_03535"
     CDS             complement(2312..2692)
                     /locus_tag="VGO02_03535"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV7476792.1"
                     /translation="MLVRAARYFVVGGISAVFDISLFFVFAKLLGYHYLAVSTVTFVF
                     AVALNYTLSVRYVFTSGVRFSRRQELALVYLVSAIGLGLHLLVLYVAVDKLGVELMLS
                     KFVATGSVFMWNFLVRNYFVFRPR"
     gene            complement(2692..3396)
                     /locus_tag="VGO02_03540"
     CDS             complement(2692..3396)
                     /locus_tag="VGO02_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV7476793.1"
                     /translation="MITLSVLIPAYNEEGTIKQVLTSVSSQRIPGVALQVIVVNDAST
                     DRTAEVVKECAGMYHTFVSLPRNGGKGAAVIAGLRAATGDYVLVQDADLEYNPNEYAG
                     LLKPVLDFGAEVVMGSRFIAPPWTRVNYFWHKVGNLTITLLFNLLNNTTFTDVYSGYL
                     MFRRSLVSPDELKRPGWDQQAEILARICARSRVLYEVPISYHGRTYEEGKKIRAHHAI
                     AVIWTMLRERFARLVA"
BASE COUNT          614 a         1056 c         1157 g          623 t
ORIGIN      
        1 ccgtgcgctc gtgctcgggc gcgtagtagt cagtcgcctt gtactgcacg agcgcatgct
       61 cggacagcac gaggaagccg tgaccgaagc cgggcggtat ccagatggcc cggtgcgtac
      121 ctccggagag cgtcgccgag acccacttgc cgaaggttgg cgagctgcgc cgcaggtcga
      181 ctgccacgtc gaagatctct cccgccaata ccgttatcag cttgccttgc ggctggcgca
      241 cctggtagtg cagcccacgc aagacattgc gcaccgagaa cgactggttg tcctgcacga
      301 actgcgggtc gagtccggtc gcttccttga agacgcgccg gttgtagctc tcgaaaaaga
      361 agccccgcgg gtcctcgaac acccgcggct cgaggatcag cacctccggt acgctggtcg
      421 gcgtgacttt catcagaaca cgcgctcggc cagcaccttg agcagatatt tgccatagcc
      481 gctgttcgcc agcggctgtg ccagcgcctt cagcttcgcg ccgctgatgt agcccatgcg
      541 ataggcgatc tcctccgggc aggcgatttt cagcccctgg cggcgctcga tggtctcgat
      601 gaagtgcgcg gcctcgagca ggctcccatg cgtgccggtg tcgagccagg cgtagccgcg
      661 gcccatcggc atgacgtcga gctggccgcg ctcgaggtac gccttgttga cgtcggtgat
      721 ctcaagctcg ccgcgggcag acggcgtcag gttcgcggcg atatcgacga cgcggttgtc
      781 gtagaagtag aggccgacca cggcgtagcg cgagcgcggc cgcttgggct tctcctcgat
      841 gccgatcgct ttgcccgtcg ggtcgaactg caccacgccg tagcgctcgg ggtcgtccac
      901 cgggtacgcg aagatcgtgg cgccgcgctt cttctgcgag gccttgttca gcagggcagt
      961 gagatcatgg ccgtggaaca ggttatcgcc gaggatcagc gcgacggggt ccttgccgat
     1021 gaacttacgc ccgaggatga aagcctgggc gattccttcc ggctttggct gcaccgcata
     1081 gcgcaggcga attccccatc gcttgccgtc gccgaggagc tcctcgaagc gcggcgtgtc
     1141 ctgcggcgtc gagatgatga ggaggtcgcg gatgccggcg agcataagcg tcgacaacgg
     1201 gtaatacacc atcggcttgt cgtagatcgg cagcaactgc ttggaaacga cctgcgttac
     1261 gggatagagg cgcgttcccg agccacccgc caggatgatc cctttcatgc cgcgcgcctt
     1321 gtcgcttcga cataccaggc tatcgtcttg ttcaggcccg attcgaagct ctctttcggc
     1381 tgccagccga gctcgcgctg gattttgcgc gaatcgatcg cgtagcggcg atcgtggccc
     1441 gggcggtcct tcacaaagga aatcaggtcg gcatagcgtc cgctgttgcg cggcgcggcc
     1501 tggtcgagaa gcgagcagag caggcggacc agatcgatgt tcgccttctc cgaattgccg
     1561 ccgatgttgt aggtctcgcc gatgcggccg cgttccagga cggcgcggat cgcgtcgcag
     1621 tggtcaagca cgtagagcca gtcgcgcacg ttcctgccgt cgccgtacac cggcaacgcc
     1681 ttgccggcga gcgcgttgtg gatgatgagc gggatcaact tctcggggaa ctgatgcggg
     1741 ccgtagttgt tggagcagtt cgtcgtgagc gtcggcaagc cgtaggtgtg gtggtaggcg
     1801 cgcaccatgt ggtcggccgc tgccttcgag gcggcgtaag ggctgttcgg cgcgtgcggg
     1861 ttctcctcgg tgaaagccgg gtcggtcggg ccgagcgagc cgtacacctc gtcggtcgag
     1921 acgtgcagga agcgcaaggc agatttactt tccttttccc aatacgaacg aaattcttcg
     1981 agaagagcga atgtcccgac gacattcgtc tgcacgaact cggccggccc gctgatcgag
     2041 cggtcgacgt ggctttcggc cgcgaaatgc acgatggcgc gcggctggtg cgcctcgagc
     2101 aggcttctca ccagcgggcg gtcgcagatg tcgccctgca cgaactggta ccgcgcgtcg
     2161 ccgcgcagcg cttcgaggtt gcgcaggttg ccggcgtagg tcagcttgtc gaggttgacg
     2221 acggcctccc cggtcgcggc aagcgtcgac aggacgaagt tcgaaccgat gaagccggca
     2281 ccgccggtga ccaggagcat ggctgcagat tctaccgagg ccggaacacg aagtagttac
     2341 ggacgagaaa attccacata aagacgctgc cggtagcgac gaacttcgac agcatcagct
     2401 ccacgccgag cttgtcgacc gctacgtaga gcacgagcag gtgcaggccg agcccgatgg
     2461 cgctcaccag gtagaccagg gcgagctcct gccgtcggct gaaacgcacc ccgctggtga
     2521 acacatagcg gacgctgagc gtgtagttga gggccaccgc gaacacgaag gtgacggtag
     2581 ataccgcgag gtagtggtag ccgagcagtt tggcgaaaac gaagaacaag ctgatgtcaa
     2641 agacggccga gatgccgccg acaacgaagt accgcgccgc ccgaacgagc atcaggcgac
     2701 gagtcgcgcg aagcgctcgc gcagcatcgt ccagatcacc gcgatggcgt ggtgggcgcg
     2761 gatcttcttg ccttcctcgt aggtgcggcc gtggtagctg atcggcactt cgtacaggac
     2821 ccgtgagcga gcgcagatac gcgccaggat ttcggcctgc tggtcccacc ccgggcgctt
     2881 gagctcgtcg ggagaaacaa gcgagcggcg gaacatcagg tacccgctgt agacgtcggt
     2941 gaaggtcgtg ttgttgagca ggttgaagag cagcgtgatg gtgaggttgc cgaccttgtg
     3001 ccagaaatag ttgacgcgcg tccagggcgg cgcgatgaag cggctgccca tcacgacctc
     3061 ggcgccgaaa tccagcaccg gcttgagcag acccgcgtac tcgtttgggt tgtattcgag
     3121 atccgcgtcc tgcaccagca cgtagtcgcc ggtcgcggcg cgcagtccgg cgatgactgc
     3181 cgcgcccttg ccgccgttgc gcggcaggga gacgaacgtg tgatacatcc cggcgcactc
     3241 cttgacgact tcggctgtcc ggtcggtgga ggcgtcgttc acgacaatca cctggagcgc
     3301 aacgcccggg atgcgttgcg aggagaccga cgtcagcacc tgcttgatgg tgccttcctc
     3361 gttgtacgcc ggaatcagga cgctcagggt gatcatcggt tcaggcgccg cgcctcgccg
     3421 acctgcgtgc gataggcttc gagcacccgc
//
LOCUS       DASYJA010000167         7507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2386203, whole genome shotgun sequence.
ACCESSION   DASYJA010000167 DASYJA010000000
VERSION     DASYJA010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7507
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2386203"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..154
                     /locus_tag="VGO02_03545"
     CDS             68..154
                     /locus_tag="VGO02_03545"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEV7476794.1"
                     /translation="MQMRLHRELKAAFDPAGILNPGRLYAQL"
     gene            complement(151..504)
                     /locus_tag="VGO02_03550"
     CDS             complement(151..504)
                     /locus_tag="VGO02_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476795.1"
                     /translation="MKQVIWLVALGIVGGLGYWIWVASRRWGERKRAAEERFASFMAQ
                     TMRPAAPKLDPLPAADLAPQKLLLEAAAKAAEAGEPALSIQLYARLIARYPGGAFATQ
                     ARAAVEAQKGKLARP"
     gene            524..1750
                     /gene="glcF"
                     /locus_tag="VGO02_03555"
     CDS             524..1750
                     /gene="glcF"
                     /locus_tag="VGO02_03555"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEV7476796.1"
                     /translation="MQTELADFIRGTPQGVEADAILRKCVHCGFCTATCPTYQVLGDD
                     LDSPRGRIYLIKRVLEGAPVSGRTRLHLDRCLTCRACETTCPSGVRYGRLVDIGREVV
                     EQRTTRGPWDWLKRAVLSFGLTRTWLFSAALRTGRSLRPLLPGSLKKRIPAETGGNTS
                     WPAPRHARKMLVLGGCVQPALAPSINAAAARVLDRLGISLIEAPGAGCCGGIRFHLNR
                     HEAGRDDMRALIDAWWPLVEARGVEAIVVTASGCGATVKDYAHLLAHDPDYRDKAARI
                     SALARDLCEVVPLDFPHIRPHGRVAFQSPCTLQHAQQLRGRVEALLARAGYQSTPVRD
                     AHLCCGSAGTYSILQPAIAAELRARKLEALQEGGAPCIATANIGCLTHLQAASAVPVR
                     HWIELVDAALERRVTT"
     gene            1761..3815
                     /locus_tag="VGO02_03560"
     CDS             1761..3815
                     /locus_tag="VGO02_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEV7476797.1"
                     /translation="MTTQVVRAACPHDCPDTCGMLVTVKDGVAVKVQGDPSMPFTEGT
                     LCTKVAHYLERTYSPDRVLFPQKRIGRKGEGRFRRIGWDQALDEIAARLKAVAEEDPQ
                     GILPCNYAGTMGMLQYSSMDRRFFHRLGASQLERTLCSSAGKAGIKATLGGSYGMDPE
                     RFDESRLILIWGSNPIVSNLHFWSRAQEAKRRGARLIAIDPYRSLTAEKCSQHIAPMP
                     GTDAALALGMMHVLIAEDLLDHDYLSKYTIGFDALKKRVLEEYTPQNVAAICGLPVED
                     VVQLGRDYGRTKPAGIRLNYGMQRHAGGGIAARTIACLPAMVGAWRDPAGGIVLTTAD
                     FYGFDHAKLERPDLLRKKSRVINHAALGDALTSAHPPVRAMIVYNNNPVAVCPDSSKV
                     VAGFAREDLFTVVMDSFMTDTADYADIVLPATTQLEHDDVHKSYGHLYVLANNAAIAP
                     LGESLPNIEVFRRLAARMGFDEPCFRDSDDDVARQALGSGHRNLEGISWEQLKERGWQ
                     RLNVAGRFAPFAQGGFPTPSGKCELHSEALAKQGIDPLPFYNPPAESVSSNPRLAARY
                     PLSFLSPPRRNFLNSSFANLPRFREADKEPEIELHPADAAQRGLRDGERVRVFNDRGS
                     FRAVARVNGKPRRGVVVALSVWWKKFAPDGKNANDVTSQRTADLGGAATFYDCLVEVE
                     KA"
     gene            3926..5176
                     /locus_tag="VGO02_03565"
     CDS             3926..5176
                     /locus_tag="VGO02_03565"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEV7476798.1"
                     /translation="MTNEIGLLRDLVETPGDLIWSLDLEGRWTYLNAAAVRFYGRQPG
                     ELIGRPFAELVARGLQERDEAVFGRVFAGEPAFDHETRHLRADGSYVDLSFNAIARRD
                     PQGRIVGAIGTARDISQHRRATAALHESIEKLRLAVEIADLDYWEWEAASDLLKWGSN
                     PEGASARTMKWSEHAALVSLEDRERYLATAREAMENGEPFDAEYRVTDADGSVLWFSA
                     RGVPMLDAAGKVQRIVGVSRDITGQKRREEGVSFLAYHDSLTGLPNRRLLQDRLDQAL
                     YSARRRDSKVGAMLIDLDDFKQVNDSGGHRTGDTVLREAAQRLGSCVRKSDTVARHGG
                     DEFVILVSDFQTETDCQVVAEKVLRVLGQPFEAEGSSYRLGASIGIAVFPSTGADGDA
                     LLRNADAAMYHAKQLGGNRYSFHG"
     gene            5199..6971
                     /locus_tag="VGO02_03570"
     CDS             5199..6971
                     /locus_tag="VGO02_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEV7476799.1"
                     /translation="MKSANPSLASVARFLAPYKLRIAAAVAALLVAAVCVLALGQGVR
                     VVVDKGFGSGEPHLLDQAVAAMIALAAVLAAATWCRFYLMMTTGERVVTDLRRAVFDH
                     ILGLDPAFFERTRTGEVISRLTNDTTLLQQVVGFGLSMFVRNALMMAGAAVMLFVTSW
                     KLALFVLLGVPATLIPILLLGRRVRRLSRANQDRVADVSAYIDEAVHEIRTVQAYAHE
                     AADRAAFARHAEAAYDAGVARIGQKAFLISSVMLIAFCAVGIILWIGGHDVLAGRLST
                     GELAAFVFYATVVASGAGTVSEVWGELQRAAGAAERLAELLQTPAQIVAPASSVEPPQ
                     RAPRAIEFDQVAFAYPARPQTRALEGFTLRVAPGERVALVGPSGAGKSTLFALLLRFY
                     DPQQGALRIDGVDVRDMDPGALRRLVAVVPQEPVIFAASVLDNVRYGRPEAGPAEVLA
                     ACERAFALEFIERLPQGLDTVLGERGVTLSGGQRQRLSIARALLADRPILLLDEATSA
                     LDAAAERLVQQALEALEQGRTTLVIAHRLATVQHADRIVVMERGAPVAQGTHAELMRQ
                     GGLYASLARLQFLDAGARPLERVA"
     gene            6974..>7507
                     /locus_tag="VGO02_03575"
     CDS             6974..>7507
                     /locus_tag="VGO02_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV7476800.1"
                     /translation="MRLILGLALAAAGVASAADGPTVGSKRFTESYLLGEILAQVSGG
                     RHKPGLGNTAILVEALRNGAIDVYPEYTGTIAREVLKSEERLDLSALNERLKPSGLAA
                     GFPLGFSNSYALGMRRADAEKLAIRRISDLRTHSTLRVGLSHEFLGRGDGWPGLQAAY
                     GLPQAPRGLVHGLAYEVL"
BASE COUNT         1130 a         2594 c         2648 g         1135 t
ORIGIN      
        1 gagggggcat gcgacgctgt ttcgcgccga gcggcgctcg ggcgaagcct tctcgccgct
       61 cgacccggtg cagatgcggc tgcatcgcga gctcaaggcg gcattcgacc ccgccggcat
      121 cctcaacccg ggccgcctgt atgcgcagct ctaaggacgc gccagcttgc ctttctgtgc
      181 ctcgaccgcg gcacgcgcct gcgtcgcgaa cgcgcccccg gggtagcgcg cgatgagccg
      241 cgcgtacagc tggatggaca atgcgggctc gcccgcctcg gccgcctttg cggccgcttc
      301 gaggagaagc ttctgcggtg ccagatcggc ggcgggcagc gggtcgagct tcggcgcagc
      361 cggcctcatg gtctgcgcca tgaagctggc aaagcgctcc tccgcggcgc gcttgcgctc
      421 tccccaccgg cgcgaggcga cccagatcca gtatccaaga cccccgacga tgcccagcgc
      481 gaccagccag atcacctgct tcatgcgaaa agtatagccg ccgatgcaga ccgagcttgc
      541 cgacttcatc cgcggcacgc cgcagggcgt ggaagccgat gccatcctgc gcaagtgcgt
      601 gcactgcggg ttctgcaccg ccacctgccc gacctaccag gtcctcggcg acgacctgga
      661 cagcccgcgc ggccgcatct acctgatcaa gcgcgtcctc gagggcgcgc cggtgagcgg
      721 caggacgcgg ctgcatctcg atcgctgcct cacctgccgc gcgtgcgaga ccacctgccc
      781 ctcgggggtg cgctacggcc gcctggtcga catcggccgc gaagtcgtcg agcagcgcac
      841 cactcgcggc ccctgggact ggctcaagcg tgccgtgctc tcgtttggtc tgactcgcac
      901 ttggcttttc tcggctgccc tgcggaccgg cagatccctg cgtccgcttc tcccggggag
      961 cctgaagaaa cgaattccgg cagagacggg aggaaacacc tcgtggccgg cgccgcgcca
     1021 cgcgcgcaag atgctggtcc tcggcggctg cgtgcagccg gcgctcgcgc cgtcgatcaa
     1081 tgcagccgcg gcgcgcgtgc tagaccggct gggcatctcc ctcattgagg ccccgggcgc
     1141 gggctgctgc ggcgggatcc gtttccacct caaccggcac gaggcggggc gcgacgacat
     1201 gcgcgcgctg atcgacgcct ggtggccgct ggtcgaggcg cgcggcgtgg aggcgatcgt
     1261 ggtgacggcg agcggctgcg gcgccacggt gaaggactat gcgcatctgc tcgcccacga
     1321 cccggactac cgcgacaagg cggcgcgcat ttcggcgctg gcgcgcgatc tgtgcgaagt
     1381 ggtgccgctg gattttccgc acatcaggcc gcatgggcgg gttgccttcc agtcgccgtg
     1441 cacgctgcag cacgctcagc agctccgcgg gcgggtggag gcgctgctgg cgcgcgccgg
     1501 ctaccagagc acgccggtgc gcgacgcgca tctgtgctgc ggctctgcgg ggacctattc
     1561 gatcctgcag ccggcgatcg cagccgagct gcgcgcgcgc aagctcgagg cgctgcagga
     1621 ggggggcgcg ccgtgcatcg ccacggcgaa catcggctgc ctcacgcacc tgcaggcggc
     1681 gagcgccgtg ccggtgcggc actggatcga gctggtcgat gcagcgctcg agcgtagagt
     1741 tacgacgtag aatcggcggc atgaccaccc aggtagtgcg ggccgcctgt ccgcacgact
     1801 gccccgacac ctgcggcatg ctggtgacgg tgaaagacgg cgtcgccgtc aaggtgcagg
     1861 gcgacccctc gatgcccttc accgagggca cgctgtgcac caaggtcgcg cactacctcg
     1921 agcgcacgta ttcgcccgac cgggtcctgt ttccccaaaa gcgaatcggc aggaaaggcg
     1981 aaggcaggtt ccgccgcatc ggctgggacc aggcgctcga cgaaatcgcg gcgcgcctca
     2041 aggcggtggc agaggaggac ccgcagggga tcctgccgtg caactacgcc ggcacgatgg
     2101 gcatgctgca gtacagctcg atggaccggc gcttcttcca ccgcctgggc gcctcgcagc
     2161 tcgagcgcac gctgtgctcg tcggccggca aggccggcat caaggcgacg ctcggcggca
     2221 gctacggcat ggacccggag cgcttcgatg aatccaggct gatcctgatc tggggctcca
     2281 acccgatcgt ctccaacctg catttctggt cgcgcgcgca ggaggccaag cgccgcggtg
     2341 ccaggctgat cgcgatcgat ccgtatcgca gcctgacggc ggagaagtgc agccagcaca
     2401 tcgcgccaat gccgggaacc gacgccgccc tcgcgctcgg catgatgcac gtgctgatcg
     2461 cagaggacct gctcgaccac gactacctct cgaagtacac gattggcttc gacgcgctga
     2521 agaagcgcgt gctggaggaa tacacgccgc agaatgtcgc ggcgatctgc gggctgcccg
     2581 tcgaagacgt ggtccagctc gggcgcgact acggccgcac caagcccgcc ggcatccgcc
     2641 tgaactacgg catgcagcgc cacgccggcg gcggcatcgc cgcgcgcacc atcgcctgcc
     2701 tgccggctat ggtgggcgcc tggcgcgacc cggctggcgg catcgttctc accaccgcgg
     2761 atttctacgg cttcgatcac gccaagctgg agcgtcccga tcttctcagg aagaaaagcc
     2821 gggtcatcaa ccacgcggcg ctcggcgacg ctctcacgtc ggcgcatccg ccggtgcgcg
     2881 ccatgatcgt ctacaacaac aacccggtcg cggtctgccc cgactcgagc aaggtcgtcg
     2941 ccggctttgc gcgcgaggac ctgttcaccg tggtgatgga ctcgttcatg accgacaccg
     3001 cggactatgc ggacatcgtg ctgccggcga ccacgcagct cgagcacgac gacgtgcaca
     3061 agtcctacgg gcacctctac gtgctcgcca acaacgcggc catcgcgccg ctcggcgagt
     3121 cgctgcccaa catcgaggtg ttccgccggc tggccgcgcg catgggcttc gacgagccgt
     3181 gcttccgcga cagcgacgac gacgtcgcgc gccaggcgct cggctccggg caccgcaatc
     3241 tcgagggcat aagctgggaa cagttgaagg agcgcggctg gcagcggctg aacgtggccg
     3301 ggcgcttcgc gccattcgcg cagggcggct tcccgacgcc ctccggaaaa tgcgagctgc
     3361 acagcgaggc gctggcgaag cagggcatcg atccgctgcc cttctacaac ccgccggccg
     3421 agtcggtgtc gagcaacccg cggctcgccg cgcgctatcc gctcagcttc ctgtcgccgc
     3481 cgcggcgcaa cttcctcaac tcgagcttcg ccaacctgcc gcgctttcgc gaggcggaca
     3541 aggagccgga aatcgagctg catccggcgg atgcggcgca gcgcggcctg cgcgacggcg
     3601 agcgcgtgcg cgtgttcaac gaccggggct ctttccgcgc ggtggcgcgc gtcaatggca
     3661 agccgcgccg cggggtggtg gtggcgctct cggtgtggtg gaagaagttc gcgccggacg
     3721 gaaagaatgc aaacgacgtg acttcgcagc gcaccgccga cctcggcggc gccgcaactt
     3781 tttacgattg tctggtcgaa gtcgagaagg cctgacagcc gttcgtcggc taatcgcaag
     3841 aagtggtcag gggtgtggcg tatccacact cgcgcgccgc ggcccacggg taccttcccg
     3901 gtcaccgaaa gaccggaagc ggaatgtgac caacgagatc ggcctgctgc gcgacctggt
     3961 cgagaccccg ggcgacctga tctggtcgct cgacctcgag gggcgctgga cctacctgaa
     4021 cgccgcggcc gtgcgcttct acggcaggca gccgggcgag ctgatcggcc gccccttcgc
     4081 cgagctggtg gcgcgcgggc tgcaggagcg cgacgaggcc gtgttcggcc gcgtcttcgc
     4141 cggcgagccg gcgttcgatc acgaaacgcg gcacctgcgc gccgacggca gctatgtcga
     4201 cctctcgttc aacgcgatcg cgcggcgcga tccccagggg cgcatcgtcg gggcgatcgg
     4261 caccgcgcgc gacatcagcc agcacaggcg cgccaccgcc gcgctgcacg aaagcatcga
     4321 gaagctgcgc ctcgcggtcg agatcgcaga cctggactac tgggagtggg aggcggcgag
     4381 cgatctgctg aagtggggta gcaaccccga gggggcgtcg gcgcgaacca tgaaatggag
     4441 cgagcacgcc gcgctggtca gcctcgagga ccgcgagcgc tatctcgcca cggcgcgcga
     4501 ggcgatggag aacggcgaac ctttcgacgc ggagtatcgg gtcaccgacg cggacggcag
     4561 cgtgctgtgg ttctctgccc gcggcgtgcc gatgctcgat gctgccggca aggtgcaacg
     4621 catagtcggc gtctcgcgcg acattaccgg gcagaagcgc cgcgaggagg gagtgtcctt
     4681 tctcgcctat cacgactcgc tcaccgggct gcccaaccgg cgcctgctgc aggaccgctt
     4741 ggaccaggcg ctgtactcgg cgcgccggcg cgacagcaag gtcggcgcga tgctgatcga
     4801 tctcgacgat ttcaagcagg tgaacgactc cggcggccat cggacagggg acacggtcct
     4861 gcgcgaagcg gcgcagcgcc tcggcagctg cgtgcgcaag tccgacaccg tggctcggca
     4921 cggcggcgac gaattcgtga tcctcgtgtc cgatttccag acggaaacgg attgccaggt
     4981 agtggccgag aaggtgctgc gcgtgctcgg gcagccgttc gaggcggagg gaagcagcta
     5041 ccggctgggc gcgagcattg gcatcgcggt gtttccctcc accggagccg acggcgacgc
     5101 gctgttgcgc aacgccgacg cggcgatgta ccacgcgaag cagctggggg gaaaccggta
     5161 cagcttccac ggctgattcg cggttaaagt cggggttgat gaagtccgcg aatccctcgc
     5221 tcgcctcggt ggcgcgcttc ctggcgccct acaagttgcg catcgcagcc gcggtggcgg
     5281 cgttgctcgt cgccgccgtc tgcgtgctgg cgcttggcca gggcgtcagg gtggtggtcg
     5341 acaaggggtt cggcagcggc gagccccacc tgctcgatca ggcggtagcg gcaatgatcg
     5401 cgctagccgc ggtactggcc gccgccacct ggtgccgctt ctacctgatg atgacgaccg
     5461 gcgagcgcgt ggtcaccgac ctgcgccgcg cggtgttcga ccatatcctc gggctggacc
     5521 cggcgttttt cgagcgcacg cgcaccggcg aggtgatttc gcgtctgacc aacgacacca
     5581 cgctgctgca gcaggtggtc ggcttcggcc tgtcgatgtt cgtgcgcaac gccctgatga
     5641 tggcgggcgc cgcggtgatg ctgtttgtca ccagctggaa gctcgcgctg ttcgtgctgc
     5701 tcggcgtgcc ggcgacgctg attccgatcc tgctcctcgg ccgtcgcgtg cggcgcctgt
     5761 cgcgcgccaa ccaggaccgc gtcgccgacg tctcggcgta catcgacgag gcggtgcacg
     5821 agatccgcac ggtacaggcc tacgctcacg aggccgccga ccgcgccgca ttcgcccgcc
     5881 atgccgaggc ggcctacgac gccggcgtcg cgcgcatcgg gcagaaggcg ttcctcatct
     5941 cgtcggtgat gctgatcgcg ttttgcgccg tgggcatcat cctgtggatc ggcggccacg
     6001 atgtcctcgc cgggcggctc tcgacgggcg agctcgccgc gttcgttttc tacgccacgg
     6061 tggtggcgag cggcgccggc acggtatcgg aggtctgggg cgagctgcag cgcgccgcgg
     6121 gcgcggccga gcgcctggcc gagctgctgc agacgccggc gcagatcgtc gcgccagcga
     6181 gcagcgtcga gccgccgcag cgcgcgccgc gcgcgatcga gttcgaccag gtcgccttcg
     6241 cctacccggc gcgcccgcaa acgcgcgcgc tcgaaggttt cacgcttcgc gtggcgcccg
     6301 gcgagcgcgt agcgctggtc ggcccctcgg gcgcgggcaa gtcgacgctg ttcgcgctgc
     6361 tgctgcgctt ctacgatccg caacagggcg cgctgcgcat cgatggcgtc gacgtgcgcg
     6421 acatggatcc tggcgcgctg cgccgcctgg tggcggtggt accgcaggag ccggtgatct
     6481 tcgccgcaag cgtgctcgac aacgtgaggt atggacggcc cgaggcgggc cctgcggagg
     6541 tgctcgccgc gtgcgagcgc gccttcgcgc tggaattcat cgagcgcctg ccgcaggggc
     6601 tcgacaccgt cctgggcgag cgtggcgtca cgctttccgg cggccagcgc cagcgcctgt
     6661 cgatcgcgcg ggcgctgctc gccgaccggc cgatcctgct gctcgacgag gcgaccagcg
     6721 cgctcgacgc cgccgcggag cgcctggtgc agcaagccct cgaggcgctc gagcaagggc
     6781 gcaccacgct ggtcatcgcc caccgcctgg ccaccgtgca gcatgccgac cgcatcgtcg
     6841 tgatggagcg cggcgcgccg gtcgcgcagg ggacgcacgc ggagctgatg agacagggcg
     6901 ggctctatgc cagcctggcg cggctgcagt tcctggacgc cggagcgcgg ccgctggagc
     6961 gggtggccta ggcatgcggc tgattctcgg cctggcgctg gcagcggcgg gcgtggcgtc
     7021 ggccgccgac ggaccgacgg tcggctcgaa acgcttcacc gagtcctacc tcctgggcga
     7081 gatcctggcg caggtttccg gcggccggca caagccgggg ctgggcaaca cggcgatcct
     7141 ggtcgaggcg ctgcgcaacg gggcgatcga cgtctacccg gagtacaccg ggaccatcgc
     7201 gcgcgaggtg ctgaagagcg aggagcggct cgatctttcc gcattgaacg agcgactcaa
     7261 gccttcgggc ctggcggcgg gatttccgct cggcttcagc aacagctacg cgctcggcat
     7321 gcgccgggcc gatgccgaga agcttgccat caggcgaatt tccgatctgc gcacacacag
     7381 cacgctgcgc gtggggctgt cgcacgagtt cctcggccgc ggcgacggct ggcccggatt
     7441 gcaggcagcc tacggcctgc cgcaggcgcc gcgcggcctg gtccacggcc tggcctacga
     7501 ggtgctc
//
LOCUS       DASYJA010000168         8501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_571214, whole genome shotgun sequence.
ACCESSION   DASYJA010000168 DASYJA010000000
VERSION     DASYJA010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8501
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571214"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="VGO02_03580"
     CDS             complement(<1..1066)
                     /locus_tag="VGO02_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV7476801.1"
                     /translation="MSNKRRDLLKAGASVAAAATFPMIWVKDAHAQWNSQPEKGAKLR
                     VLRWSRFVQGDIDQYMKNVAAFTAKYGIEVRVDNESWEDVRPKAAVAANTGAGPDIIL
                     GTNDDANLYPEKLVDVTDLANYLGKKYGGWYDAGAAYLRPNGKNWIGIPLGAAGSMMV
                     YRESQVKAAGFDGIPKDTDGFLKLMKALAAKGTPGGFALGHATGDANTWCHWVVWAFG
                     GKMVDKSGKVVIDSPETIKALEYSKELYGIFAPGTLSWLDPSNNKAFLDGQISVTNNG
                     ISIYYAAKTSKDPKINALAADINHSVYPAGPGGSNAAFHLFLNQMIFKYTKYPRAAKE
                     FLRFMMEEEQFGPWMQASIGY"
     gene            complement(1077..2123)
                     /gene="ugpC"
                     /locus_tag="VGO02_03585"
     CDS             complement(1077..2123)
                     /gene="ugpC"
                     /locus_tag="VGO02_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEV7476802.1"
                     /translation="MAAVALSAVRKSFGSTEVVHGVDVSIADGEFCVLVGPSGCGKST
                     LLRMIAGLEEISGGEVRIGSRVVNKVAPKERDIAMVFQNYALYPHMTVYDNMAFSLKL
                     AHLPADEVRSRAHEAAQILGLMDYLERYPRQLSGGQRQRVAMGRAIVRKPQVFLFDEP
                     LSNLDAKLRVSMRTEIKALHQRLKTTSIYVTHDQIEAMTMADRIVVMNAGRVEQIGSP
                     LELYDNPVNLFVAGFIGSPAMNFVAGKVNGAGISVGGGIDLPNPTRNLDAGRSVLVGV
                     RPEHLAVDPGGVPVEVVVVEPTGADTQIFCKLAGTDVTAVVRERHTFRPGEAVRLKPQ
                     LTYLFDPSSGARLA"
     gene            complement(2131..3858)
                     /gene="araD"
                     /locus_tag="VGO02_03590"
     CDS             complement(2131..3858)
                     /gene="araD"
                     /locus_tag="VGO02_03590"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HEV7476803.1"
                     /translation="MSKKKPEDLRSHRWYGVNDLRSFGHRSRTAQMGYDRSDYAGKPV
                     IAIINTWSDINSCHTHFKQRVEEVKRGVWQAGGFPVEMPAMSLAEVMQKPTTMMYRNF
                     LAMETEELLRSYPADGAVLMGGCDKTTPALVMGAISMNLPAIYMPAGPMLSGHWRENS
                     LGSGSDTWKYWAELRAGNITERDWKEIEDGIARSPGTCMTMGTAATMMSVTEALGLAL
                     PGASSIPAPDSNHAKMATLCGKRAVEMVWEDLKPRDILKKESFDNAIVTLMAMGGSTN
                     ALIHLVAMAGRAGIKLPLERYNEYSAKTPLLANVRPSGDKYLMEDFYYAGGLRALLAE
                     LKDLLHLDCRTVNGMTLGENLQGARIFNEEVIRKRSNPLKASGGLVVVRGNLAPNGAV
                     IKAAAATPRLLKHVGKAVVFEDYNDMAARIDRDDLEVDADSVLILRNAGPLGGPGMPE
                     WGMLPVPKKLLKQGVRDMVRISDARMSGTSYGTCVLHVAPESFVGGPLAFVRNGDLIE
                     LDVEKRLLNLKISPEEMQIRKSAWKPPARKYERGYGAIFATHVKQADEGCDFDFLEGT
                     APTPEPEIH"
     gene            complement(3851..4816)
                     /locus_tag="VGO02_03595"
     CDS             complement(3851..4816)
                     /locus_tag="VGO02_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7476804.1"
                     /translation="MKVVAVALAFLTFLFCSAVQAQTYPSRPIRLIVPYAAGGTSDIL
                     ARQIGPKLTDAWGQPVVVENRTGANGNVGADFVAKSAPDGYTLLITDLGGLVISASVY
                     PKLPFDPSKDFSPVVMVSYSPHVLAVHPSVPAKSMKELIELAKAQPGKLNFAVSGIGG
                     APQLAGIEFAQRAGVRWTYIPYKGGSDAVAAVAAGQADVLFNGMLATWGTVQGGRLRA
                     LAVSSAQRVPTAPDTPTVAEQGMAGFETGSFQGVVGPAGMPRETVSKLNAELIKVLSS
                     EEMKERFAKQGTEVRTGTPDSLGQWLRTEQARWAKVVKESGEKFE"
     gene            complement(4848..5867)
                     /locus_tag="VGO02_03600"
     CDS             complement(4848..5867)
                     /locus_tag="VGO02_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEV7476805.1"
                     /translation="MSTNRREFLAAAGAVAAVVAAPKALAQWQPSQRYPDPSIKIIDQ
                     SFGRYRLALAKVERIASGMRWCEGPVWFGDGRYLLWSDIPNNRIMKWDEESGAVSVFR
                     KPSNNANGNTRDRQGRLLTCEHDTRRVTRTEYDGTITVIADRYDGKPLNSPNDIVCKS
                     DGSIWFTDPPFGILGFYEGHVEKPELPTNVYRWDPKTQKLAVVAGDVNRPNGLAFSPD
                     EKRLYIIEAGVTPRVVRVYDVVDGGARLSNGKVFLTAEAGGTPDGFRVDVDGNLWMGW
                     GMGSEALDGVNVFNSEAKLIGRIDLPERSANLCFGGRYRNRLFMCGSTSVFSLYVNTQ
                     GAAGG"
     gene            5964..6911
                     /locus_tag="VGO02_03605"
     CDS             5964..6911
                     /locus_tag="VGO02_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7476806.1"
                     /translation="MAGLFFALCAGSAAAQGFPSKPIRIVIPFVAGGPSDTVGRAIGS
                     KFQELLGQPAVVENKPGANGSIAAEFVAKSDADGYTILVGSIGVFSINAALYSDLRYD
                     PVRDFAPITLAVTNPNVLITKLDFAPRSVKELVEYAKKNPGKISYGSSGTGSSDHLTS
                     ELFKQMSGTYGVHIPYRGGAAVITDLLGGQIEASFQNLGNVVTNVKAGKIRALAVTGR
                     TRAPQLPDVPTMAESGYPDLIVTSWQAAAAPAKTPRDIVARLNEASVKALRSPDVRER
                     LTQIGFDVVAGTPEEFGKFMKDEVDRWTRVVKRGGIKPD"
     gene            6911..7981
                     /locus_tag="VGO02_03610"
     CDS             6911..7981
                     /locus_tag="VGO02_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HEV7476807.1"
                     /translation="MSLVFRKESLEKAIEALAAAGGSEPVEARLVAENLVTANLVGHD
                     SHGIGMMPRYVEALLEGGLHANQKPEVKLDAGALLVLDGRAGYGQTIGRDATMMAISR
                     AKQHGTSITALGNSHHLGRIGHWAEMAVAEGLISMHFVNVRSFARVAAYGGADRKFGT
                     NPVCIAIPLPGEPPFVLDMATSAVAQGKLRVAHNKRVKVPKGWLIDDQGNPSDDPRWG
                     VVPPLGAMLTFGGHKGYGLAVACDLLGGALTGGGADAYENKTQRRVLNGMLSILIDPQ
                     RLGTQASFEKETRDYLAWLRASRPAPGFDRVRIAGEPERETRAKREKEGIPVDQETWK
                     EILAAAGKLKLAPEKIEALARA"
     gene            complement(8004..>8501)
                     /locus_tag="VGO02_03615"
     CDS             complement(8004..>8501)
                     /locus_tag="VGO02_03615"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7476808.1"
                     /translation="SISAQRILARAGSASRLAMRGICGISSIVHLSMKHGCYAARRML
                     SMTQADDMKLLEQLNRNYVRSVQESDVRWFDENLTPDFMNSNPDGTLVDRAGFLAQIA
                     RPVTIAGLKAQDVRIRILGDTAIIHARTTYRKPDGQDSAGRYTDIWQRRGTRWLCVAA
                     HVTRV"
BASE COUNT         1343 a         2832 c         2822 g         1504 t
ORIGIN      
        1 cgtagccgat gctcgcctgc atccatggcc cgaactgctc ctcctccatc atgaagcgca
       61 ggaactcctt cgccgccctc gggtacttgg tgtacttgaa gatcatctgg ttgaggaaca
      121 ggtggaacgc cgcgttcgag ccgccgggcc cggccggata gaccgagtgg ttgatgtccg
      181 cggccagcgc attgatcttc ggatctttcg aggtcttggc cgcgtagtag atcgagatgc
      241 cgttgttggt caccgagatc tgcccgtcga ggaacgcctt gttgttgctc gggtcgagcc
      301 aagacagcgt gccgggcgcg aagatgccgt agagctcctt cgagtactcg agcgccttga
      361 tggtctcggg gctgtcgatc accaccttgc ccgacttgtc caccatcttg ccgccgaagg
      421 cccacaccac ccagtggcac caggtattgg cgtcgccggt ggcgtggccg agcgcgaagc
      481 cccccggcgt gcctttcgca gccagcgcct tcatcagctt caggaaaccg tcggtgtcct
      541 tcgggatgcc gtcaaagccc gccgctttca cctgactctc gcgatacacc atcatgctgc
      601 cggccgcgcc gagcgggatg ccgatccagt tcttgccgtt ggggcgcagg taggccgcgc
      661 ccgcgtcgta ccagccgccg tacttcttgc cgaggtagtt ggcgaggtcg gtgacgtcga
      721 ccagcttctc cgggtagagg ttggcgtcgt cgttggtgcc caagatgatg tcgggcccgg
      781 cgccggtatt cgcggcgacc gctgccttcg gacgaacgtc ctcccagctc tcgttgtcga
      841 cgcgcacctc gatgccgtac ttcgcagtga acgccgcgac gttcttcatg tactggtcga
      901 tgtcgccctg gacgaagcgg ctccagcgca gcacgcgcag cttcgcgccc ttctcgggct
      961 ggctgttcca ctgggcgtgc gcgtccttca cccagatcat cgggaacgtc gcagccgcgg
     1021 cgacgctcgc tcctgcctta agcaagtccc tgcgtttgtt gctcatcact cctcctttat
     1081 gcaagtcgag cgccgctcga cggatcgaaa agataggtca gctgcggctt gaggcgcacc
     1141 gcctcgccgg ggcgaaacgt atgccgttcc cgcaccaccg ccgtgacatc ggtcccggcg
     1201 agcttgcaga agatctgcgt atcggccccg gtgggctcca ccactaccac ctcgaccggc
     1261 acgccgccgg ggtcgaccgc caggtgctcc gggcgcacgc cgaccaggac cgagcggccg
     1321 gcgtcgaggt tgcgggtcgg gttgggcagg tcgatgccgc cgcccactga gatgccggcg
     1381 ccgttcacct tgccggcgac gaaattcatc gccggcgagc cgatgaagcc ggcgacgaac
     1441 agattcaccg ggttgtcgta gagctcgagc ggcgagccga tctgctcgac gcggcctgcg
     1501 ttcatgacca cgattctgtc ggccatggtc atcgcctcga tctggtcgtg cgtgacgtag
     1561 atcgaggtgg tcttcagccg ctggtgcagc gccttgatct cggtgcgcat cgacacgcgc
     1621 agcttcgcgt cgaggttgga gagcggctca tcgaacagga acacctgggg cttgcgcacg
     1681 atggcgcggc ccatggcgac gcgctggcgc tgcccgccgg agagctggcg cggatagcgc
     1741 tcgaggtagt ccatcaggcc gaggatctgc gccgcctcgt gcgcgcggct gcgtacttcg
     1801 tcggccggca ggtgggcgag cttgaggctg aacgccatgt tgtcgtacac cgtcatgtgc
     1861 gggtacaggg cgtagttctg gaacaccatc gcgatgtcgc gctctttggg cgccaccttg
     1921 ttcaccacgc ggctgccgat gcgcacctcg ccgccggaga tctcctccag gccggcgatc
     1981 atgcgcagca gcgtcgactt gccgcagccc gacgggccga ccagcacgca gaactcgccg
     2041 tcggcgatcg atacgtcgac gccgtgcacg acttcggtgg agccgaacga cttgcggacg
     2101 gcgctgagag cgactgctgc catgagccgg ctagtgtatc tccggctcgg gtgtcggggc
     2161 ggtgccctcg aggaaatcga aatcgcagcc ttcgtcggcc tgcttcacgt gcgtcgcgaa
     2221 gatggcgccg tagccgcgct catatttccg tgccggcggc ttccaggcgc ttttgcgtat
     2281 ctgcatctct tccggggaaa tcttgaggtt caaaagcctt ttttcgacgt cgagctcgat
     2341 cagatcgccg ttgcgcacga aagcaagcgg gccgccgaca aaggactcgg gcgcgacgtg
     2401 cagcacgcag gtgccgtagc tggtgccgct catcctggca tccgagatgc gcaccatgtc
     2461 gcgcacacct tgcttgagga gcttcttggg caccggcagc atgccccact cgggcatgcc
     2521 gggaccgccg agcgggccgg cattgcgcag gatcagaacc gagtcggcgt cgacctcgag
     2581 gtcgtcgcgg tcgatgcgtg ccgccatgtc gttgtagtcc tcgaacacca ccgctttgcc
     2641 gacgtgcttg agcaggcgcg gcgtggcggc cgcggccttg atgaccgcgc cgttcggcgc
     2701 aagattgccg cgcaccacga ccagcccgcc ggacgccttc agaggattgc ttcgcttcct
     2761 gatgacctcc tcgttgaaga tccgggcgcc ctgcaggttc tcgcccagcg tcatgccgtt
     2821 gacggtccgg caatccaggt gcagcaggtc tttcaattcc gcaagcagcg cccgcagccc
     2881 gcccgcatag tagaaatcct ccatcaggta cttgtcgccc gaggggcgca cgttggcgag
     2941 cagcggggtc ttcgccgagt actcgttgta ccgctcgagc ggcagcttga tccccgcgcg
     3001 gcctgccatg gcgacgaggt ggatcagggc attggtcgag ccgcccatcg ccatgagcgt
     3061 cacgatggcg ttgtcgaaag actctttttt caggatatcg cgtggcttga ggtcctccca
     3121 caccatctcg accgcgcgct tgccgcagag cgtcgccatc ttcgcgtggt tggagtccgg
     3181 cgccgggatc gaggaggcgc ccggcagcgc taggccgagc gcctcggtga cggacatcat
     3241 ggtcgcggcg gtgcccatgg tcatgcaggt gccgggcgag cgcgcgatgc cgtcctcgat
     3301 ttctttccag tcgcgctcgg tgatgttgcc ggcgcgcagc tccgcccagt atttccaggt
     3361 gtcggagccc gagccgagcg agttttcccg ccagtggccc gagagcatcg gcccggccgg
     3421 catgtagatc gccggcaggt tcatgctgat ggcccccatc accagcgccg gcgtggtctt
     3481 gtcgcagccg cccatcagca ccgcgccgtc ggccggatag gagcgcagca gctcctcggt
     3541 ctccatggcg aggaagttcc ggtacatcat ggtggtgggc ttctgcatca cttcggcgag
     3601 cgacatcgcc ggcatctcca ccgggaagcc gcctgcctgc cacacgccgc gcttcacttc
     3661 ctcgacgcgc tgcttgaagt gcgtgtggca ggagttgatg tccgaccacg tgttgatgat
     3721 cgcgatgacc ggcttgccgg cgtagtcgct gcggtcgtag cccatctgcg cggtgcgcga
     3781 gcggtggccg aacgagcgca ggtcgttcac cccgtaccag cggtggctac gcaggtcttc
     3841 gggtttcttc ttactcaaat ttttctccgg attccttgac gactttcgcc cagcgggcct
     3901 gctcggtgcg tagccactgc cccaggctgt ccggcgtgcc ggtgcgcacc tcggtgccct
     3961 gcttggcgaa gcgctctttc atctcctcgc tgctcaggac ctttatcaat tccgcattca
     4021 gcttcgatac ggtctcgcgc ggcatgccgg cgggcccgac cacgccctgg aacgagccgg
     4081 tctcgaaccc ggccatgccc tgctcggcca ccgtgggcgt gtccggggcg gtgggcacgc
     4141 gctgcgcgct cgagaccgcg agcgcacgca gcctgccgcc ctgcaccgtg ccccaagtgg
     4201 cgagcatgcc gttgaacagc acgtcggcct gccccgcggc caccgctgcg accgcgtcgg
     4261 aaccgccttt gtacgggata taggtccagc gcacgccggc gcgctgcgcg aactcgatac
     4321 ccgcgagctg cggcgcgcca ccgatgccgg agacggcgaa gttcagcttg ccgggctgcg
     4381 cctttgccag ctcgatcagc tctttcatgc tcttcgccgg caccgacgga tggaccgcca
     4441 ggacgtgcgg cgagtacgag accatcacca ccggcgagaa atccttggac gggtcgaacg
     4501 gcagcttggg atagacgctc gcgctgatca ccagcccgcc caggtcggtg atcagcaggg
     4561 tgtagccgtc gggcgcgctc ttcgcgacga aatcggcgcc gacgttgccg ttggcgccgg
     4621 tgcggttctc caccaccacc ggctggcccc aggcgtcggt gagcttcgga ccgatctggc
     4681 gcgcgaggat gtcggaggtg ccgccggcgg cataggggac gatcaggcgg atcggcctgg
     4741 acggataggt ctgggcctga accgcgctgc aaaaaagaaa tgtaagaaat gcgagcgcaa
     4801 ccgcaaccac cttcatggcg gttcctcctc ctgaaaagag accgagatta tcccccggcg
     4861 gcgccctgcg tgttgacgta gagcgaaaac accgacgtgc tgccgcacat gaagaggcgg
     4921 ttgcgatagc gcccgccgaa gcacaggttg gcgctgcgtt cgggcaggtc gatgcggccg
     4981 atcagcttgg cctcgctgtt gaagacattg acgccgtcca gcgcctcgct gcccatgccc
     5041 cagcccatcc agaggttgcc gtcgacgtcg acgcggaaac cgtcgggcgt gccgccggcc
     5101 tcggcggtga ggaacacttt gccgttggac aggcgcgcgc cgccgtcgac cacgtcgtag
     5161 acgcgcacca cgcgcggcgt cacgccggcc tcgatgatgt agagccgctt ttcatcgggc
     5221 gagaaggcga ggccattcgg gcggttgacg tcgcccgcga cgaccgcgag cttctgcgtc
     5281 ttcgggtccc agcggtagac gttggtcggc agctcgggct tctcgacgtg gccctcgtag
     5341 aagccgagga tgccgaaggg cgggtcggtg aaccagatcg agccgtctga cttgcagacg
     5401 atgtcgttcg gcgagttgag cggcttgccg tcgtagcggt cggcgatcac cgtgatcgtg
     5461 ccgtcgtact cggtgcgcgt aacgcgccgt gtgtcgtgct cgcaggtgag caggcggccc
     5521 tggcggtcgc gcgtgttgcc gttcgcgttg ttcgagggct tgcggaacac gctgaccgcg
     5581 ccgctctcct cgtcccactt catgatgcgg ttgttcggga tgtcgctcca tagcaggtag
     5641 cggccgtcgc cgaaccacac cggcccttcg caccagcgca tgcccgaggc gatgcgctcg
     5701 accttggcga gggcgaggcg gtagcgcccg aagctctggt cgatgatctt tatggatgga
     5761 tccggatagc gctggctcgg ctgccattgc gcgagcgctt tcggcgcggc gacgacggct
     5821 gcgacggctc ccgccgccgc caggaattcc ctgcggttgg tcgacatggt tcccctccct
     5881 ctagctcgca gtctagcgcc gtgtcccgaa gcaagtaaac tcgcaggctt ccgaatagag
     5941 gaggcgagag aatgctgcga gcgttggctg gcttgttctt tgcgctgtgc gccggtagcg
     6001 ccgccgccca gggcttcccg tccaagccga tccgcatcgt gatcccgttc gtcgccggcg
     6061 ggccgtccga caccgtcggg cgcgccatcg gctccaagtt ccaggagctc ctcggccagc
     6121 cggcggtagt cgagaacaag cccggggcca acggctccat cgccgcggag ttcgtcgcca
     6181 agtcggatgc cgacggctac acgatcctgg tcggctcgat cggcgtgttc tcgatcaacg
     6241 ccgcgctcta cagcgacctg cgctacgacc cggtgcgcga tttcgcgccg atcacgctgg
     6301 cggtcaccaa cccgaacgtg ctcatcacca agctcgattt cgcgccgcgc agcgtgaagg
     6361 agctggtgga gtacgcgaag aagaatcccg gcaagatctc ctacggctcg agcggcaccg
     6421 gctcgtcgga ccacctgacc tccgagctgt tcaagcagat gagcggcacc tacggcgtgc
     6481 acatcccgta ccgcggcggc gccgcggtga tcaccgatct gctcggcggc cagatcgagg
     6541 cttccttcca gaacctcggc aacgtcgtca ccaacgtgaa ggcgggcaag atccgcgcgc
     6601 tcgcggtgac cggccgtacg cgcgcgccgc agctgcccga cgtgccgacg atggccgaat
     6661 ccggctaccc cgacctgatc gtcacttcct ggcaggcggc cgcggcgccg gcgaagacgc
     6721 cgcgcgacat cgttgcgcgc ctgaacgagg cgtcggtcaa ggcgctgcgc tcgcccgatg
     6781 tgcgcgagcg gctgacgcag atcggcttcg acgtagtggc aggcacgccg gaggagttcg
     6841 gcaagttcat gaaggacgaa gtcgatcgct ggacccgcgt agtaaaacgt ggcggcatca
     6901 agcctgactg atgagtttgg tttttagaaa agagtcactg gaaaaagcca tcgaggcgct
     6961 cgccgccgcc ggcggcagcg agccggtcga ggcgcggctg gtcgccgaga acctggtcac
     7021 cgccaatctg gtcggccacg attcgcacgg catcggcatg atgccgcgct atgtggaggc
     7081 gctgctcgag ggcggcctgc acgccaacca gaagccggag gtcaagctcg acgccggcgc
     7141 gctgctggtg ctcgatggcc gagccggtta cgggcagacg atcggacgcg acgcgacgat
     7201 gatggccatc tcgcgcgcca agcagcacgg cacctcgatc accgcgctgg gcaactcgca
     7261 ccacctcggc cgcatcggcc attgggccga gatggcggtg gccgagggcc tgatctcgat
     7321 gcatttcgtg aacgtgcgct cgttcgcgcg cgtcgccgcc tacggcggcg ctgaccgcaa
     7381 gttcggcacc aatcccgtat gcatcgcgat tccgctgccc ggcgagccgc cgttcgtcct
     7441 cgacatggcg acgagcgccg tagcacaggg caagctgcgc gtcgcccaca acaagcgggt
     7501 caaggtgccg aagggctggc tgatcgacga ccagggcaac ccgagcgacg atccgcgctg
     7561 gggcgtggtg ccgccgctcg gcgcgatgct caccttcggc gggcacaagg gatacgggct
     7621 cgcggtagcc tgcgacctcc ttggcggcgc tctcaccggt ggcggcgccg acgcctacga
     7681 aaacaagacg cagcgccgcg tgctcaacgg catgctgtcg atcctgatcg acccgcaaag
     7741 gctcggcacg caggctagct tcgagaaaga gacgcgggac taccttgcgt ggctgcgcgc
     7801 atcgcgcccc gcgccgggct tcgaccgcgt gcgcatcgcg ggcgagccgg agcgcgagac
     7861 gcgagcaaag cgcgaaaagg aaggcattcc cgtggaccaa gagacctgga aggaaatcct
     7921 cgccgccgcc gggaagctca agctcgcgcc ggagaagatc gaggcgctcg cgcgcgcttg
     7981 agcgcttgcg cgaaccttcg cggtcagacg cgggtgacgt gcgcggcgac gcacagccag
     8041 cgcgtgccgc gccgctgcca gatgtctgta tagcgcccgg cgctgtcctg cccgtcgggc
     8101 ttccggtagg tcgtgcgcgc atggatgatc gcggtgtcgc cgaggatgcg gatacgcaca
     8161 tcctgtgcct tgagcccggc gatggtcacc ggcctggcaa tctgcgccag aaagccggcg
     8221 cgatcgacca gcgtgccgtc cgggttgctg ttcatgaagt cgggcgtcag gttctcgtcg
     8281 aaccagcgca cgtcggattc ctgcaccgag cggacgtaat tccggttgag ctgctccagc
     8341 agcttcatgt cgtcggcttg cgtcatggac agcattctac gtgcggcgta gcatccatgt
     8401 ttcatggaga gatgaactat ggatgagatg ccgcagatcc cgcgcatcgc caacctgctc
     8461 gccgacccgg cgcgcgccag gatcctctgg gcgctgatcg a
//
LOCUS       DASYJA010000169         4545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1206806, whole genome shotgun sequence.
ACCESSION   DASYJA010000169 DASYJA010000000
VERSION     DASYJA010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206806"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="VGO02_03620"
     CDS             <1..1100
                     /locus_tag="VGO02_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEV7476809.1"
                     /translation="PGIASFALRFGPPEFFAVYLLTFCSFVGLGREEKHKTIISMTLG
                     LLLAGVGMDTVSGQLRMTFGLDELLRGINFLVAVIGLFGISEILLTMEERLALRGHAA
                     SISLRVVLKVWKEMPRYWVTLLRSSVIGCWLGITPGGAIAASFMGYNLAKRFSKDPGS
                     FGKGRIEGVFAPETAAHASGTSALLPMLALGIPGSGTAAILLGGLMVWGLNPGPLLFV
                     EHKDFVWGLIASMYLGNVVGLVIVLTTVPLFAAVLRVPFSAIAPMIVVSCAIGAFAIQ
                     NAMFDIWLMLLFGVVGYVFKKIGIPLAPFTLALVLGNRAEDSFRLSMIGAGGDLRVFW
                     SNGLVGSITTLALILLFWPLISAALARLRRR"
     gene            1097..1876
                     /locus_tag="VGO02_03625"
     CDS             1097..1876
                     /locus_tag="VGO02_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV7476810.1"
                     /translation="MSGEVLLSRDGDVATLTLSNAERLNALSLAMWEQLGAHMKKLDA
                     DESLRCIVMRGAGDKAFAAGADIANFAAERANSKQAKEYGTRIAGSMRALAECRHPVV
                     ALIQGACVGGGLLVASQADIRICGESARFGVPVKNLGLVEAYDELQGMMRVVGRAATL
                     EILLEGRIWGAREAYEKGLVNRVAPDDKAPEEAYAIARRIAEGAPLAARWHKKFVRRL
                     ADTRPLTEAEFDESYACFDTEDFREGVAAFLAKRKPRFKGK"
     gene            1884..2795
                     /locus_tag="VGO02_03630"
     CDS             1884..2795
                     /locus_tag="VGO02_03630"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEV7476811.1"
                     /translation="MSRPRLAFFVGDPNGIGPELAAKLLARPETRAAADVKVFGDPRL
                     VECDMEVVPGLEADALTPGLPNPAAGKWVIDALTRMALAARAREVDGIVFGPLNKQAM
                     KLGGLAYNDEQHLFAQLLQAEGYSVEFNVIDQLWTSRVTSHIPLKDVAGSITVQGVVD
                     AVRIIHEALRRGGNARPRVAVAGLNPHAGEGGTMGREEIDIIAPAVERARREGIAATG
                     PLPADTVFVAAKRGDFDAVVTMYHDQGQIAMKLMGFDRGVTVLGGLPVPIATCAHGTA
                     YDIVGRNSANPVPLVNAFQLAARMASH"
     gene            2805..3755
                     /locus_tag="VGO02_03635"
     CDS             2805..3755
                     /locus_tag="VGO02_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7476812.1"
                     /translation="MRSLALLLAFACFSAHAQPFPSRPVRIMVGSAAGGGTDIISRLL
                     AAKLQEAWGQPVLVENRTGASASIAADFVAKSPPDGYTVVMAVPNSHTIAPHLIKLPY
                     DPLRDFTPITLAVQVPHVLVVSQSSPVSSMRELVDLARANPGKLNFSSSGVGATQHLA
                     GELFNLVTGVQTVHVPYKGSAAAMGDLIAGAVTMSFDTTASSIGNIRAGKLKALAVAA
                     PVRASALQDVPTTAEAGFPGVEMITWYGLFGPPNMLRALTEKWQQDVARALALPDIKE
                     RVASLAGEPGGNRPEEFAAYIRDQYAKMGKLVKDAGVRAE"
     gene            3850..>4545
                     /locus_tag="VGO02_03640"
     CDS             3850..>4545
                     /locus_tag="VGO02_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEV7476813.1"
                     /translation="MKRVFLFVLAALMSSAVQGQAWPNKPVTLVNPFASGGGVDAFGR
                     PLSVVLSRNLGTQFIVENLAGAGGTVGAASAAKRPGDGYNFFLGAVHHTIAESLYAKL
                     PYSLERDFVPVTVLAYVPNVVVMHPKNAQIGSIRELIAFAKANPGKLNYGSAGNGTTH
                     HLAGELFKTMAGVDLTHIPYKGAGPMMQDLLAGQVDMAFDGMGTSAPQIKAGKLKALA
                     VTTAQRSFVDPSVP"
BASE COUNT          713 a         1537 c         1524 g          771 t
ORIGIN      
        1 ctcccggcat cgcctcgttc gccttgcgct tcggcccgcc ggaattcttc gccgtctact
       61 tgctcacgtt ctgctcgttc gtcggcctgg gccgcgagga gaagcacaag accatcattt
      121 ccatgacgct cggcctgctg ctcgccgggg tgggcatgga tacggtctcg ggccagctgc
      181 gcatgacgtt cggcctggac gagctgctgc gcggcatcaa cttcctggtt gccgtgatcg
      241 gcctgttcgg catcagcgag atcctgctga ccatggaaga gcgcctcgcg ctgcgcggcc
      301 acgccgcgag catcagcttg cgcgtggtgc tgaaggtgtg gaaggaaatg ccgcgctatt
      361 gggtcaccct gctgcgctcg tcggtgatcg gctgctggct cggcatcacc ccgggcggcg
      421 cgatcgccgc ctctttcatg ggctacaacc tcgccaagcg cttctccaag gacccggggt
      481 cgtttggcaa aggacgcatc gagggtgtgt tcgcgccgga gacggcggcg cacgcctcgg
      541 gcacttccgc gctgctgccg atgctcgcac tcggtatccc gggctccggc accgcggcga
      601 tcctgctcgg cggcctgatg gtgtggggcc tcaatcccgg accgctgctc ttcgtcgagc
      661 acaaggactt cgtctggggc ctgatcgcct cgatgtatct tggcaatgtc gtcgggctgg
      721 ttatcgtcct caccaccgtg ccgctcttcg ccgccgttct gcgcgtgccg ttctcggcga
      781 tcgccccgat gatcgtggtg tcctgcgcga tcggcgcttt cgccatccag aatgcgatgt
      841 tcgacatctg gctgatgctc ctcttcggcg tggtcggcta cgtgttcaag aaaatcggca
      901 ttccgctcgc cccgttcacc ctcgccctgg tgctcggcaa ccgcgccgag gactcgttcc
      961 gcctgtcgat gatcggcgcc ggcggcgacc tgcgggtgtt ctggtccaac ggcctggtcg
     1021 gctcgatcac cacgctcgcg ctgatcctgc tgttctggcc gctgatctcc gccgcgctcg
     1081 cgcggctgcg gcgccggtga gcggcgaggt cctgctctcg cgcgacggcg acgtcgcgac
     1141 gctcacgctt tccaatgccg agcgcctgaa cgcgctttcg ctggcgatgt gggagcagct
     1201 cggcgcccac atgaaaaagc tcgatgcgga cgagtcgctg cgctgcatcg tgatgcgcgg
     1261 cgccggcgac aaggcatttg ccgccggcgc cgatatcgcg aacttcgccg ccgagcgcgc
     1321 caactcgaag caggccaagg agtacggcac gcgcatcgcc ggcagcatgc gcgccctcgc
     1381 cgagtgccgt catccggtgg tggccctgat ccagggcgca tgcgtgggcg gcggtctgct
     1441 ggtggcctcg caggcggaca tccgcatctg cggcgagtcg gcgcgcttcg gtgtgccggt
     1501 aaagaacctc ggcctggtgg aggcttacga cgaactgcag ggcatgatgc gcgtggtcgg
     1561 ccgcgctgcg acgctcgaaa ttctgctcga gggccgcatc tggggcgcgc gcgaagccta
     1621 tgagaaaggc ctggtgaacc gcgtcgcccc cgatgacaaa gccccggagg aagcctatgc
     1681 catcgcgcgg cgcattgcag aaggcgcgcc gctggcagcg cgctggcaca agaagttcgt
     1741 gcgccgcctg gccgatacgc gcccgctcac cgaggcggag ttcgacgaga gctacgcttg
     1801 cttcgacacc gaggatttcc gcgagggcgt ggcagcgttt ctcgccaagc gaaagccgcg
     1861 gttcaaggga aaatgagggg cacatgagca ggccgcggct ggcttttttc gtcggcgacc
     1921 cgaatggcat cggacccgag ctcgccgcca agctgctggc gaggcctgaa acccgggcgg
     1981 cggccgatgt gaaggtgttc ggcgacccgc gccttgtcga gtgcgacatg gaggtcgtcc
     2041 ccggcctcga ggcagacgcg ctcacgccgg gcctccccaa ccccgctgcg ggcaaatggg
     2101 tcatcgacgc gctcacccgc atggcgctcg ccgcacgcgc gcgcgaggtc gacggcatcg
     2161 tattcgggcc gctcaacaag caggcgatga agctcggcgg cctggcgtac aacgacgagc
     2221 agcatctgtt cgcgcagctg ctgcaggccg agggctacag cgtcgagttc aacgtcatcg
     2281 accagctgtg gacctcccgc gtcacttccc atatccccct caaggacgtg gccggctcaa
     2341 ttaccgtgca aggcgtggta gacgcggtgc gcatcatcca cgaagcgctg cgccgaggcg
     2401 gcaacgcgcg gccgcgcgtc gcggtcgccg gcctcaaccc gcacgccggc gaaggcggaa
     2461 cgatgggacg cgaggagatc gacatcatcg cgcccgccgt ggagcgagcg cggcgcgagg
     2521 gaatcgcggc cacgggaccg ctgcccgccg acaccgtgtt cgtcgcggcg aagcgcggtg
     2581 acttcgacgc cgtggtgacc atgtaccacg accagggcca gatcgcgatg aagctgatgg
     2641 gattcgaccg cggcgtgaca gtgctgggcg gactgcccgt gccgatcgcc acctgcgccc
     2701 acggcactgc ctacgacatc gtcgggcgca acagcgcgaa cccggtcccg ctcgtcaatg
     2761 cgttccaact cgccgcccgc atggcgtccc attaggagat cccgatgagg tcgcttgcgc
     2821 tgcttctcgc gttcgcgtgt ttctccgcgc acgcccagcc tttcccttcg cgcccggtgc
     2881 gcatcatggt ggggtcggcc gcgggcggcg gcaccgatat catctcgcgc ctgctcgccg
     2941 ccaagctgca ggaagcgtgg ggccagccgg tgctggtcga gaaccgcacc ggagcgagcg
     3001 cctcgatcgc cgccgacttc gtagccaaga gcccgccgga cggatacacg gtggtgatgg
     3061 ccgtgccgaa ctcgcatacc atcgcgccgc atctcatcaa gctgccctac gacccgctgc
     3121 gcgatttcac gccgatcacg ctcgcggtgc aggtgccgca cgtgctggtg gtcagccaga
     3181 gctcgcccgt gagcagcatg cgcgagctgg tcgatctcgc cagggcgaac cctggcaagc
     3241 tcaatttctc gtcttccggc gtcggcgcca cgcagcacct ggcgggcgag ctgttcaatc
     3301 tggttaccgg ggtccaaact gtgcacgttc cctacaaggg gagcgccgcg gcgatgggcg
     3361 acctgatcgc gggcgcggtg accatgagtt tcgataccac cgcctcctcc atcggcaaca
     3421 tccgcgcagg caagctgaag gcgctcgcgg tcgcggcgcc ggtgcgcgcc tcggcgctgc
     3481 aggatgtacc gaccaccgcc gaagccggat ttcccggcgt cgagatgatc acctggtacg
     3541 gcctgttcgg accgccgaac atgctgcgcg cgctcaccga gaagtggcaa caggacgtag
     3601 cgcgtgcgct ggcgctgccc gatatcaagg agcgcgtcgc gagcctcgca ggcgagcccg
     3661 ggggcaaccg tccggaggag ttcgcggcgt acatccgcga ccagtacgcg aagatgggca
     3721 agctggtaaa ggacgccggc gtacgcgcgg aatagccgcc aaggccatct cgggcgtttc
     3781 gcaggcggtg cagtagcagc ctgttattat tcgaaaaaaa tacggaccgg ggagttgcga
     3841 ggaggagtca tgaagcgagt ttttcttttc gtcctggcgg cgctgatgag ctccgccgtg
     3901 caggggcagg catggccgaa caagccggtc acgctggtca atccattcgc gtctggcggt
     3961 ggcgtcgatg ctttcggcag gccgctctcg gtcgttctga gccgcaatct cggcacgcag
     4021 ttcattgtcg agaacctcgc cggcgccggc ggcaccgtgg gcgcggcgag cgccgcaaag
     4081 cggccaggcg atggctacaa cttctttctc ggcgcggtgc accacaccat cgccgagagc
     4141 ctctacgcga agctccccta ctcgctcgag cgcgacttcg tgccggtcac cgtgctcgcg
     4201 tatgtgccga acgtggtcgt gatgcatccg aagaacgccc agatcggctc gatcagggaa
     4261 ctgatcgcct tcgccaaggc gaacccgggc aagctgaact acggctcggc cggcaacggc
     4321 accacgcacc atctcgccgg cgagttgttc aagaccatgg ccggcgtgga tctcacccac
     4381 atcccgtaca agggcgccgg tccgatgatg caggacctgc tcgccgggca ggtggacatg
     4441 gcgttcgacg gcatgggtac ctcggcgccg cagatcaagg ccggcaagct gaaggcgctc
     4501 gccgtgacca cggcgcagcg ctcgttcgtc gacccgagcg tgccg
//
LOCUS       DASYJA010000170         2326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1388315, whole genome shotgun sequence.
ACCESSION   DASYJA010000170 DASYJA010000000
VERSION     DASYJA010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388315"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /gene="puuE"
                     /locus_tag="VGO02_03645"
     CDS             complement(<1..746)
                     /gene="puuE"
                     /locus_tag="VGO02_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HEV7476814.1"
                     /translation="MHYPRDLVGYGARPPKVRWPGNARVALNFVLNYEEGGENSVLHG
                     DRAAEAFLSEIVGAQPVEGARHMSMESIYEYGSRVGVWRILSLFARHKLPLTVYGVAM
                     AMERNPAAVEAMLEAGHEIASHGWRWISYQHVPLEVEREHMQRAIEIHQRLTGERPLG
                     WYTGRTSPNTRKLVVEDGGFVYDADDYNDDLPWYDSRYGRPQLVVPYTLDVNDMRFAT
                     AQGFNSGDDFFSYLKDSFDVLYAEGNRMMS"
     gene            complement(730..1959)
                     /locus_tag="VGO02_03650"
     CDS             complement(730..1959)
                     /locus_tag="VGO02_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HEV7476815.1"
                     /translation="MLSPERIMQRCDSLARHSELPGGLTRVFLSPQSRAAADLVLGWM
                     REAGMQAALDAIGNVRGRYEGERPGLPCLMLGSHLDTVRDAGRYDGMLGVVSAIECVA
                     ALNSQKKRLPFAIEVVGFGDEEGVRFGTTLLGSRAVAGNFDFSSLKVKDSNSVSMEQA
                     LRDFGLEPEKIPLVAKKKGEVLAYAELHIEQGPVLEAEGLPVGVVTAINGFSRLRVTL
                     SGTAGHAGTVPMNLRRDALVAAAECIAAVEKNAKTYNDVVATVGRIETSPDAINVIPG
                     KVVFTVDVRAPRDELRFSCLASVQEEIKAICRARNIGIEIENLQEFGVTRCAERLMAQ
                     MERAVAAEGLRVRRLPSGAGHDGMAIRAIADIAMLFVRCKGGISHNPAESITTDDAAA
                     GARVLCRFIENFNALSA"
     gene            1999..>2326
                     /gene="uraD"
                     /locus_tag="VGO02_03655"
     CDS             1999..>2326
                     /gene="uraD"
                     /locus_tag="VGO02_03655"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:TIGR03164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEV7476816.1"
                     /translation="MNVPEDKQAFVARFGGIFEHSPWVAERAWQRRPFASLDSLHAAM
                     LQVVSQAQKQEQLALVRAHPELAGREAKAGELTADSSGEQGRLGFTSLDRDEFLKMQQ
                     INRAYRE"
BASE COUNT          373 a          781 c          774 g          398 t
ORIGIN      
        1 atcgacatca tccggttgcc ttcggcgtac aggacatcga agctgtcctt aagataggaa
       61 aagaaatcgt cccccgagtt gaatccttgc gcggtggcga agcgcatgtc gttgacgtcc
      121 agcgtgtagg ggacgacgag ctgcggcctg ccgtagcggc tgtcgtacca cggcaggtcg
      181 tcgttgtaat cgtcggcgtc gtagacgaag ccgccgtcct cgaccaccag cttgcgcgtg
      241 ttcgggctgg tgcgcccggt gtaccagccg agcggccgct cgccggtgag ccgctgatgg
      301 atctcgatgg cgcgctgcat gtgctcgcgc tcgacctcca ggggcacgtg ctggtagctg
      361 atccagcgcc agccgtggct cgcgatctcg tggccggcct cgagcatcgc ctcgaccgcg
      421 gcgggattgc gctccatcgc catggcgacg ccatagacgg tgaggggcag cttgtgccgg
      481 gcgaacaggc tgaggatgcg ccagacgccg acgcgcgagc cgtactcgta gatcgactcc
      541 atcgacatgt ggcgcgcgcc ctcgaccggc tgcgcgccga cgatctcgga gaggaaggcc
      601 tcggctgccc tgtcgccgtg caggaccgag ttctcgccgc cttcctcgta gttgaggacg
      661 aagttgagcg cgacgcgggc gttgccgggc cagcgcactt tcggcggcct ggcgccgtag
      721 ccgaccaggt cacgcggata gtgcattgaa gttctctatg aagcggcaaa gcaccctggc
      781 gccggccgcc gcgtcgtcgg tcgtgatcga ctcggccggg ttgtggctga tgccgccctt
      841 gcagcgcacg aagagcatcg ctatgtcggc gatggcgcgg atcgccatgc cgtcgtggcc
      901 ggcgcccgag ggcaggcggc gcacccgcag cccttccgcg gcgacggcgc gctccatctg
      961 cgccatcagc cgctccgcgc agcgcgtgac gccgaattcc tgcagattct cgatttcgat
     1021 gccgatgttt ctcgcgcggc aaatcgcctt gatttcctcc tgcacggagg ccaggcatga
     1081 aaaacgcaac tcgtccctcg gcgcgcgcac gtccaccgtg aacaccacct ttccgggaat
     1141 cacgttgatc gcgtcggggc tcgtctcgat ccgcccgacg gtcgcgacga catcgttgta
     1201 tgtttttgcg tttttttcga cggcggctat gcactccgcg gcagccacca gcgcgtcgcg
     1261 ccgcaggttc atcggcaccg tgccggcgtg accggccgtg cccgagagcg tgacgcgcag
     1321 gcgcgagaag ccgttgatcg ccgtcaccac gcccaccggc aggccctcgg cttcgagcac
     1381 cggcccctgc tcgatgtgca gctcggcata ggcgagcacc tcgcccttct tcttcgccac
     1441 tagaggaatc ttttccggct cgaggccgaa atcgcgcaac gcctgctcca tgctgacgct
     1501 gttcgaatct ttgactttca gcgaagaaaa atcgaaattg cctgcgacgg cccggctgcc
     1561 gagcagcgtc gtgccgaagc gcacgccttc ctcgtcgccg aagccgacga cctcgatcgc
     1621 gaagggcagc cgtttcttct gcgagttcag cgcagccacg cactcgatgg cgctcaccac
     1681 gccgagcatg ccgtcgtagc ggccggcgtc gcgcaccgtg tcgaggtgcg agccgagcat
     1741 caggcacggc aggccgggcc gctcgccctc gtagcggccg cgcacgttgc cgatggcgtc
     1801 gagcgcggcc tgcatgccgg cttcgcgcat ccagccgagc acgaggtcgg ccgcggcgcg
     1861 gctttgcggc gagaggaaaa cgcgcgtcag gccgccgggc agctcggagt ggcgggcgag
     1921 cgagtcgcag cgctgcatga tccgttcggg ggaaagcatc gcgcaaatag tataaatgcc
     1981 acgtatattg ccgccgacat gaatgtcccg gaggacaagc aggcgttcgt cgcgcgtttc
     2041 ggcggcatat tcgagcattc gccgtgggtc gccgagcgcg cgtggcaaag gcggccgttc
     2101 gcttccctcg actcgcttca cgccgccatg ctgcaagtcg tttcgcaggc gcagaagcag
     2161 gagcagctgg cgctggtgcg cgcgcacccc gagctagccg ggcgcgaggc gaaggccggc
     2221 gagctgaccg cggattcctc gggcgagcag gggcgcctcg gcttcacgtc tctcgacagg
     2281 gacgaattcc tcaaaatgca gcagatcaac cgcgcctacc gcgaga
//
LOCUS       DASYJA010000171         5040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2842567, whole genome shotgun sequence.
ACCESSION   DASYJA010000171 DASYJA010000000
VERSION     DASYJA010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2842567"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1576
                     /gene="metG"
                     /locus_tag="VGO02_03660"
     CDS             <1..1576
                     /gene="metG"
                     /locus_tag="VGO02_03660"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEV7476817.1"
                     /translation="ENCSTVYSATALKNPYSTLSGARPLLKTSEHHFFKLSDPKCKAF
                     IQQWLEAPGRLQSQVVNKAKEWLEGTGDKTLTDWDISRDPPYFGIRIPDIKDEKYLYV
                     WLDAPVGYLASLKNYCAKKKLDFEKLLSDPQTEQIHFIGKDIIYFHTLFWPAMLKFAG
                     YKIPDHVWVHGFITVSGDKMSKSRGTGISPLRYLELGMNPEWLRYYIAAKLNANVEDL
                     DFNPDDFLARVNSDLVGKYVNIASRAAAFISRQFDGALQESKTDGSWTDAVAIGDQVA
                     AAYESREFGKVVREVMAVADRVNQRFDQAKPWELAKDPARREELHRVCSDAIHAFFTL
                     SVYLAPMLPATASRIARELFALSRDLSFEDRKLRPGRIQPFKHLLTRVEDKQLDALFE
                     PPAQNLTQPQPTKEAAAVDNSTIAIDDFNKLDLRVARIARAEQVEGADKLLKLTLDLG
                     DGTRTVFAGIKSAYAPEKLEGRLTVVVANLAPRKMKFGVSEGMVLAASADSAGSEPGI
                     FLISPDAGARPGMRVK"
     gene            1617..2723
                     /locus_tag="VGO02_03665"
     CDS             1617..2723
                     /locus_tag="VGO02_03665"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7476818.1"
                     /translation="MVESTSRTLVCSVLFLDIAEYSRKPVAEQLQLKQAFNRILGEAL
                     EQVAPRDRIILDTGDGAAVTFMGDPEDALFAAMSMRDMCGTVPVRSGVNLGPVRLVKD
                     LNGQMNIIGDGINVAQRVMSFSRPGQLLVSRSFYEVVSCLSRDYANLFHHEGSRTDKH
                     VREHEVYSVVGGTPTARRVSETESKFSALANGSGLLAGHGPLGLRRTSLAAAGALFLA
                     LIGTGLAARAVMGPGSDAPAEKPAAAVPKAAKVASAPPSAPAEAVKKAPVESKPLAKA
                     DKPATKPVVVAAAPQSAPAAAKATASRIELAVAPWGEVFVDGKSRGVSPPLRTLELSP
                     GSHTIEIRNSTFPARVEKVVVKPGDAVKIRHRFK"
     gene            2720..3136
                     /locus_tag="VGO02_03670"
     CDS             2720..3136
                     /locus_tag="VGO02_03670"
                     /inference="COORDINATES: protein motif:HMM:NF038027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TssQ family T6SS-associated lipoprotein"
                     /protein_id="HEV7476819.1"
                     /translation="MRRLVMPVTALALLAGCDTAPMKEFRSDAKSAWAQITGSSTSNK
                     GAPALSTGLKQYDDGAYEPAAKSLQTALDQGLPDKQKVTAHKHLAFINCSQSRNGPCR
                     DEFRKALAIDPSLELTPAEAGHPTWGPIFRSLKAGR"
     gene            3144..4484
                     /locus_tag="VGO02_03675"
     CDS             3144..4484
                     /locus_tag="VGO02_03675"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV7476820.1"
                     /translation="MEAPKLGRYAIVSELGRGAMGVVYKATDSVLERTVAIKTVNMNL
                     TPEEAKGYEARFYQEARSAGSLNHPNIVTIYDVGRVNDVVYMAMEFIEGVELRSLFAE
                     GRPMPVTQAISIAAQVAEGLGYAHDHGVVHRDIKPANIMVVQNGPVKITDFGIARMRA
                     SSQELTQTGMMLGSPKYMSPEQVIGKRADHRSDIFSLGVILYEMLCGATPFNGENVTA
                     LMYQIVNFAPPAPSSINSAVPELLDYIVAKMLAKPIDERYQNGHEVSRDLRQCEQQLG
                     INVGATQPPVRTVPLPGLAAGAQPELALPQAEAKVIAQEVSRTRGVDAADPAAEGAAT
                     PARGVSHSFDSLEATQRLAVLTSNAAAALASSKPPPAPMTGTSPSTFEVGPTPARSSA
                     DIAAETERQTATQAIGSIPRPANRTGWRRRDWLLVGGATLAGVVLAGVILKRRD"
     gene            complement(4496..4873)
                     /locus_tag="VGO02_03680"
     CDS             complement(4496..4873)
                     /locus_tag="VGO02_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476821.1"
                     /translation="MVAGLALAGFLVVAVVLPQMAGAETKDAAQALLAGVDAAQKQVT
                     ERAEKAGNLSGSGKEVKIAVKNDPKHGELKWLVAENGAIRGWNEKNAIEITLTPSMAG
                     SKMAWSCKGYPMSAMPTTCGGRS"
BASE COUNT          876 a         1790 c         1615 g          759 t
ORIGIN      
        1 cgagaactgc agcacggtgt attccgccac cgccctgaag aacccctatt cgacgctctc
       61 cggcgcgcgg ccgctgctca agacctcgga gcatcacttc ttcaagctgt cggatccgaa
      121 gtgcaaggcg ttcatccagc aatggctgga agcccccggc cggctgcagt cccaggtcgt
      181 caacaaggcg aaggagtggc tggagggaac cggcgacaag acgctgacgg actgggacat
      241 ctcgcgcgac ccgccctact tcggcatccg catccccgac atcaaggacg agaagtacct
      301 ctacgtctgg ctcgacgcgc cggtcggcta cctcgccagc ctgaagaact actgcgcgaa
      361 gaagaagctg gacttcgaga aacttctttc cgacccgcag accgagcaga tccacttcat
      421 cggcaaggac atcatctact tccacaccct cttctggccg gcgatgctca agttcgccgg
      481 ctacaagatc cccgaccacg tctgggtgca cggcttcatc accgtctcgg gcgacaagat
      541 gtcgaagtcg cgcggcaccg gcatcagccc gctgcgctac ctcgagctcg gcatgaaccc
      601 cgagtggctg cgctactaca tcgcggccaa gctgaatgcc aacgtcgagg acctcgactt
      661 caaccccgac gacttcctcg cccgcgtcaa cagcgacctg gtcggcaagt acgtgaacat
      721 tgcgagtcgg gccgcggcct tcatctcgcg gcagtttgat ggtgctttgc aggaatcaaa
      781 aacagatggc agctggacag atgccgtggc catcggcgac caagtcgctg ccgcgtacga
      841 gtcgcgcgaa ttcggcaagg tggtgcgcga ggtgatggcg gtggccgacc gcgtcaacca
      901 gcgcttcgac caggcgaagc cctgggagct cgccaaggat ccggccaggc gcgaggagct
      961 gcaccgcgtg tgcagcgatg cgatccacgc cttcttcacg ctctcggtct atctcgcgcc
     1021 gatgctgccg gcgactgcct cgcgcatcgc ccgcgagctc tttgcgctga gccgggatct
     1081 cagcttcgag gaccgcaagc tgcgccccgg gcgcatccag ccgttcaagc acctgcttac
     1141 ccgggtggag gacaagcagc tcgacgcgct gttcgagccg cccgcacaaa atttgacgca
     1201 gccgcagccg acaaaggaag cagccgccgt ggacaacagc acgatcgcca tcgacgactt
     1261 caacaagctc gacctgcggg tggcgcgcat cgcgcgcgcc gaacaggtcg agggggccga
     1321 caagctcctc aagctgacgc tggacctcgg cgacggcacg cgcacggtgt tcgccgggat
     1381 caagtcggcc tacgccccgg aaaagctgga ggggcgcctg acggtggtgg tggcgaacct
     1441 cgcgccccgc aagatgaagt tcggggtgtc ggagggcatg gtcctggctg cgtcggcaga
     1501 cagcgccggc tcggaaccgg gcatttttct catttctccg gacgcgggag cgcggcccgg
     1561 catgcgcgta aaatagcgcc gtcgcggccg tagagccgca ttaccggcaa aaaaccgtgg
     1621 tcgagagcac cagccgaacg ctggtctgca gcgtcctatt cctcgacatc gccgagtatt
     1681 cgaggaagcc ggtcgccgag cagcttcagc tcaagcaggc gttcaaccgc atcctgggcg
     1741 aggccctcga gcaggtggcg ccgcgcgacc gcatcatcct cgacaccggc gacggcgcgg
     1801 cggtgacgtt catgggcgac ccggaggacg cgctgttcgc ggcgatgtcg atgcgcgaca
     1861 tgtgcggcac ggtgccggtg cgctcgggcg tcaacctcgg gccggtgcgc ctggtgaagg
     1921 acctcaacgg gcagatgaac atcatcggcg acggcatcaa cgtcgcgcag cgggtgatga
     1981 gcttctcgcg gcccgggcag ctgctcgtgt cgcgctcgtt ctacgaagtg gtgtcgtgcc
     2041 tgtcgcgcga ctacgccaac ctgttccacc acgaaggctc gcgcaccgac aagcacgtgc
     2101 gcgagcacga ggtgtactcg gtggtcggcg gcacgccgac ggcgcgccgc gtgtcggaaa
     2161 ccgaatcgaa gttctcggcg ctggccaacg gttcgggcct gctcgccggc cacggccccc
     2221 tcggcctgcg ccgcacctcg ctcgccgccg ccggtgcgct gttcctcgcc ctcatcggca
     2281 ccggcctcgc ggcgcgcgcc gtaatgggtc ccggctccga tgcgcccgcc gagaagccgg
     2341 ccgcagccgt gcccaaggcg gcgaaggtgg cgagcgcccc gcccagcgcc cccgccgaag
     2401 cggtgaagaa ggcgccggtc gaaagcaagc cgctcgccaa ggcagacaag ccggctacca
     2461 agcccgtggt ggtcgctgcc gcgccgcaga gcgcacccgc cgccgccaag gcgacggcgt
     2521 cccgcatcga gctcgcggtc gccccatggg gcgaggtgtt cgtcgacggc aagagccgcg
     2581 gcgtgagtcc gccgctgcgc acgcttgaat tatctcccgg ctcgcatacg atagagattc
     2641 gcaactcgac cttcccggct cgcgtggaga aggtcgtggt caagccgggg gatgcggtga
     2701 agattcggca cagattcaaa tgaggcgact ggtgatgccc gtgacggcgc tcgcgctgct
     2761 cgccggctgc gataccgcgc ccatgaagga gttccgcagc gacgcgaaga gcgcctgggc
     2821 gcagatcacc ggctcgtcca cgtccaacaa gggcgccccg gcgctcagca caggactcaa
     2881 gcagtacgac gacggcgcct atgagccggc ggcgaagagc ctgcaaaccg cgctcgacca
     2941 gggtctgccc gacaagcaga aggtcaccgc gcacaagcac ctcgccttca tcaactgctc
     3001 gcagagccgc aacgggccgt gccgcgatga gttccgcaag gccctcgcca tcgatccttc
     3061 cctggagctg acgcccgccg aagccggaca ccccacctgg ggtccgatct tccgctccct
     3121 gaaggccggg cgctaagcgg gagatggagg caccaaagct cggccgctac gccatcgtgt
     3181 ccgagctcgg acgcggtgcg atgggcgtcg tctacaaggc gaccgactcg gtgctcgagc
     3241 gcaccgtcgc catcaagacg gtcaacatga acctgacgcc ggaggaagcg aagggctacg
     3301 aggcgcgctt ctaccaggag gcgcgctcgg caggcagcct gaaccatccc aacatcgtca
     3361 ccatctacga cgtcggccgc gtcaacgacg tcgtctacat ggcgatggag ttcatcgagg
     3421 gcgtcgagct gcgcagcctg ttcgccgagg gccgcccgat gccggtgacg caggcgatct
     3481 cgatcgcggc gcaggtggcc gagggcctgg gctacgcgca cgaccacggc gtggtgcacc
     3541 gcgacatcaa gccggccaac atcatggtgg tgcagaacgg cccggtgaag atcaccgact
     3601 tcggcatcgc gcgcatgcgc gcctcgtcgc aggagctcac gcagaccggc atgatgcttg
     3661 gctcgcccaa gtacatgtcg cccgagcagg tgatcggcaa gcgcgccgac caccgctcgg
     3721 acatcttctc gctcggcgtc atcctctacg agatgctgtg cggcgcgacg ccgttcaacg
     3781 gcgagaacgt gaccgcgctc atgtaccaga tcgtcaattt cgcgccgccg gcgcccagct
     3841 cgatcaacag cgccgtgccc gagctgctcg actatatcgt cgccaagatg ctcgccaagc
     3901 cgatcgacga gcgctaccag aacggccacg aagtgtcgcg cgacctgcgc cagtgcgagc
     3961 agcagctcgg catcaacgtc ggcgccacgc agccgccggt gcgcaccgtg ccgcttcccg
     4021 gcctcgccgc cggcgcgcag cccgagctcg ccctgccgca agccgaggcg aaggtcatcg
     4081 cgcaggaagt gagccgcacg cgcggcgtcg acgccgccga tcccgccgcc gagggcgccg
     4141 cgacgccggc gcgcggcgtg tcgcattcgt tcgattcgct cgaggcaacg cagcggctgg
     4201 cggtgctgac cagcaacgcc gccgcggcgc ttgcgtcgag caagccgccg cctgcgccca
     4261 tgacgggaac ctcgcccagc accttcgaag tcggcccgac gcccgccagg tcctcggccg
     4321 acatcgccgc cgagacggag cgccagaccg cgacgcaggc catcggctcg atcccgcgcc
     4381 ccgctaatcg caccggctgg cgccggcgcg actggctgct ggtgggaggc gctacgcttg
     4441 ccggggtggt gctcgcgggc gtgatcctca agcgccgcga ctgaagctca ggcgctcagg
     4501 agcggccgcc gcaggtcgtc ggcatggcgc tcatcgggta gcccttgcag ctccaggcca
     4561 tcttgctgcc cgccatcgaa ggggtgagcg tgatctcgat ggcgttcttc tcgttccagc
     4621 cgcggatggc gccgttctcc gccaccagcc acttcagctc gccgtgcttg gggtcgttct
     4681 tcaccgcgat tttcacttcc ttgcccgagc cgctcaggtt gcccgccttc tcggcgcgct
     4741 cggtgacctg cttctgcgcc gcgtcgaccc cggcgagcag cgcctgcgcc gcgtccttcg
     4801 tctccgcgcc cgccatctgc ggcagcacca ccgcgaccac caggaatccg gccaatgcga
     4861 ggccggcgac catcaacaga acaatccgca ttcccatccc cctgtctcgg caatccgggc
     4921 gagcttacca ttcacccgag tgactttcac cctcgcattt cacgccttct gagatgccca
     4981 ctcttcatct gcgcatcgtc ggcatggtcc agggcgtcgg ctaccgctac gcactccagg
//
LOCUS       DASYJA010000172         3620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3114375, whole genome shotgun sequence.
ACCESSION   DASYJA010000172 DASYJA010000000
VERSION     DASYJA010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3114375"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2781)
                     /locus_tag="VGO02_03685"
     CDS             complement(<1..2781)
                     /locus_tag="VGO02_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV7476822.1"
                     /translation="MRRFNFFQRNEGTFVALAVIGGLAIVPFAVNNFVQGRLLLGAFN
                     AAIAAWFLAHGISVYRGRRVLPPPAVFAPAIAMLAYAMWARGEVGIFWAYPAVLLFHF
                     ILQRRAANYFNSTVMVFTTVFAGLSYGPDVALRVGVTLFLTIVFANVFSFVSETQRGR
                     EAEQEQLLELERDRLALLVHATQTGFTDWDTRANVVIYSERFKEMLGYPAGFDTSAWR
                     SFFDLMHPADHVRVHEVFRTLMRVKRKPGLQPPGEPLEYRLQRAGGGNVWVKAASLAQ
                     VDSAGRIRRFITSFQDITKYREQEASLRTEQRRLDLVVRASRAGIVDWDGRTRATWYS
                     PRFREMLGYAAHADTSAWPDYFKVLIHPDDRSRVTARFREYIRGSGPEGPVEHYDPEE
                     YRLLRADGRYVWVQSQGVCVRDEKGFVTRFIAAITDITDRRDQDKFSADVFDALPIGL
                     AMRDLEGRYVFVNRAWEEYTGSRRADVIGKKVRERAPKEEADRVEAEDREALERGADA
                     PPQLKDLQYGDKRYVLTRTVITDANGAPRGVVVASLDTTERHAMEEKLVGEQRRAALI
                     VRAANVGILDWDGIKRTVYYSPRFREILRYPPDADTAGWPDYFDMVHPEDKERVHRSF
                     REHIRSGGPEGSEEVVHELIQYRLRRADGTYVWVEAFGASVRDVRGFATRFIASISDI
                     SERRFQEEALRSAVRLREEVERMSRHDLKTPINSVIAMSRLLREEGRLGREDDEVLGS
                     IERAGYRVLNMVNLSLDLFRMEQGSYEFRPRPVDMAEVARRVAADLESQAASKGVAVR
                     VRAEGARSAHAEEPLCYSMLANLIKNAIEAAPDDSEVGVSVEAVEGDALAVRVHNDGA
                     VPEVVRPRFFQKYTTAGKSGGTGLGTYSARLLARIQQGDISLATSEAEGTTLTVKLAL
                     AAAPAATPAEGPA"
     gene            complement(2819..>3620)
                     /locus_tag="VGO02_03690"
     CDS             complement(2819..>3620)
                     /locus_tag="VGO02_03690"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEV7476823.1"
                     /translation="PASPRTGEPRTGLSMGEHCEEMAKEWQIGRREQDELALASHKNA
                     AAAWKDGFFEDLVFPFMGNSKDNNVRADTTLEKLAVLRNAFDPSPAGTLTAGNSSPLT
                     DGAACVLLASEEWASSKKIPVQAYLTFTETAAVDFVGMAGPKEGMLMAPAYAVPRMLD
                     RAGLQLQDFDFYEIHEAFAAQVLCTLKAWQSEEYCLSRLGRRALGSINLTKLNTKGSS
                     VAIGHPFAATGARIIATLAKQLAQRGSGRGLISICAAGGQGVTAILER"
BASE COUNT          544 a         1252 c         1254 g          570 t
ORIGIN      
        1 cgccgggcct tcggccggcg tcgcagccgg cgcggcggcg agagcgagct tcaccgtcag
       61 cgtggtgccc tccgcctccg aggtggcaag cgaaatgtcg ccctgctgga tgcgcgcgag
      121 caggcgcgcc gagtaggtgc ccaggcccgt gccgccgctc ttgccggcgg tggtgtactt
      181 ctggaagaag cgcggccgca ccacctcggg cacggcgccg tcgttgtgca cgcgcacggc
      241 gagcgcgtcg ccctcgaccg cttcgacgct cacgccgacc tcgctgtcgt cgggcgccgc
      301 ctcgatcgcg ttcttgatca ggttggcgag catcgagtag cacagcggct cctccgcgtg
      361 cgcgctgcgc gcgccctcgg cgcgcacccg cacggcgact cctttggagg cggcctggct
      421 ctcgaggtcg gcggcgacgc ggcgcgccac ctcggccatg tcgaccggcc gcggccggaa
      481 ctcgtagctg ccctgctcca tgcggaacag gtcgagcgac aggttcacca tgttcagcac
      541 gcggtagccg gcgcgctcga tgctgccgag gacctcgtcg tcctcgcggc ccaggcgtcc
      601 ttcctcgcgc agcaggcgcg acatggcgat cacgctgttg atcggcgtct tcaggtcgtg
      661 ccgcgacatg cgctcgacct cctcgcgcag ccgcaccgcc gagcgcagcg cctcctcctg
      721 gaagcggcgc tcgctgatgt cgctgatcga ggcgatgaaa cgggttgcaa aaccacgaac
      781 atcgcgcacc gacgcgccga aggcctccac ccagacgtag gtgccgtcgg cgcggcgcag
      841 gcggtactgg atcagctcgt gcaccacctc ctcgcttcct tccgggccgc cgctgcggat
      901 gtgctcgcgg aagctccggt gcacgcgctc cttgtcctcc gggtgcacca tgtcgaaata
      961 gtcgggccag cccgcggtgt cggcgtcggg cggatagcgc aggatctccc tgaagcgcgg
     1021 cgagtagtag acggtgcgct tgatgccgtc ccagtcgagg atgccgacgt tggcggcgcg
     1081 cacgatcagc gcggcgcggc gctgctcgcc gaccagcttc tcctccatcg cgtggcgctc
     1141 ggtggtatcg aggctcgcca ccaccacgcc gcgcggcgcg ccgttcgcgt cggtgatcac
     1201 ggtgcgcgtc agcacgtagc gcttgtcgcc gtactgcagg tccttcagct gcggcggggc
     1261 gtccgcgccg cgctcgaggg cttcgcggtc ctcggcttcg acccggtcgg cttcctcctt
     1321 cggcgcgcgc tcgcgcacct tcttgccgat cacgtccgcg cgccgcgagc cggtgtactc
     1381 ctcccaggcg cggttgacga acacgtagcg cccctcgagg tcgcgcatgg cgagcccgat
     1441 cggcagagcg tcgaacacgt cggcggagaa cttgtcctgg tcgcgccggt cggtgatgtc
     1501 ggtaatcgcg gcgatgaagc gcgtgacgaa ccccttctcg tcgcgcacgc acacgccctg
     1561 cgactgcacc cacacgtagc ggccgtcggc gcgcagcagc cggtactcct ccgggtcgta
     1621 gtgctcgacc gggccttcgg gaccgctgcc gcggatgtac tcgcggaagc gcgcggtgac
     1681 gcggctgcga tcgtcggggt ggatcaggac cttgaagtag tccggccagg ccgaggtatc
     1741 ggcgtgggct gcgtagccga gcatctcgcg gaagcgcggc gagtaccagg tggcgcgcgt
     1801 gcgcccgtcc cagtcgacga tgccggcgcg cgaggcgcgc accaccaggt cgaggcggcg
     1861 ctgctcggtg cgcaggctgg cttcctgctc gcggtacttg gtgatgtcct ggaaggaggt
     1921 gatgaagcgc cggatgcggc ctgcggagtc gacctgcgcg aggctggcgg ctttcaccca
     1981 gacgttgccg ccgccggcgc gctgcaggcg gtactcgagc ggctcgcccg gcggctgcag
     2041 ccccggcttg cgcttcacgc gcatcagcgt gcggaacacc tcgtgcaccc ggacgtggtc
     2101 cgccggatgc atcaagtcga agaaggagcg ccacgcagag gtgtcgaagc cggccgggta
     2161 gccgagcatc tccttgaagc gctcggagta gatcaccacg ttcgcgcgag tgtcccagtc
     2221 ggtaaagccg gtctgcgtgg cgtgcaccag cagcgcgagg cggtcgcgct ccagctcgag
     2281 cagctgctcc tgctcggcct cgcgcccgcg ctgcgtctcg ctgacgaagg agaacacgtt
     2341 ggcgaagacg atggtcagga acagcgtgac cccgacgcgc agcgcgacgt ccgggccgta
     2401 gctcagcccg gcgaagacgg tggtgaacac catcaccgtg gagttgaagt agttggcggc
     2461 gcgccgctgc aggatgaagt ggaacagcag caccgccggg taggcccaga agatgccgac
     2521 ctcgccgcgc gcccacatgg cgtaggccag catggcgatc gcgggcgcga acaccgccgg
     2581 cggcggcagc acgcgccggc cgcggtagac cgagatgccg tgcgccagga accaggcggc
     2641 gatcgcggcg ttgaacgcgc cgagcagcag ccgcccctgc acgaagttgt tgacggcgaa
     2701 gggcacgatc gccagcccgc cgatcacggc gagcgcgacg aaggtgccct cgttgcgctg
     2761 gaagaagttg aagcgcctca ccgcgcaatg atagagagga aacgcttcct gaccgaattt
     2821 atcgttccag gatggcggtg acgccctgcc cgccagcggc gcagatcgag atcagcccgc
     2881 gccccgagcc gcgctgggcg agctgcttgg cgagggtggc gatgatgcgc gcgccggtgg
     2941 cggcgaaggg atgcccgatg gccacgctcg atcctttggt gttcaattta gttaaattaa
     3001 tagaacctag agcacgccgc ccaagcctgc tcaggcagta ctcctccgac tgccaggcct
     3061 tgagcgtgca cagcacctgc gcggcgaagg cctcgtggat ctcgtagaag tcgaaatcct
     3121 gcagctgcag cccggcgcga tcgagcatgc gcggcaccgc gtaggccggc gccatcagca
     3181 tgccctcctt cggcccggcc atgccgacga agtcgaccgc ggcagtctcg gtaaacgtca
     3241 gatatgcctg cactggaatt tttttcgaag acgcccattc ctcggaagcg agcagcacgc
     3301 acgccgcgcc gtcggtgagc ggcgagctgt tgcccgccgt cagggtcccg gctggagacg
     3361 gatcgaaggc gttcctcagg acggccagct tttccagcgt tgtgtccgcg cgaacattgt
     3421 tgtccttcga atttcccatg aaggggaaaa cgagatcctc gaaaaagccg tccttccagg
     3481 ccgcggccgc gttcttgtgg ctggccagcg cgagctcgtc ctgctcgcgc cgcccgatct
     3541 gccactcctt cgccatctcc tcgcagtgct cgcccatcga caggccggtg cgcggctcgc
     3601 cggtgcgcgg cgatgcaggt
//
LOCUS       DASYJA010000173         6425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2569437, whole genome shotgun sequence.
ACCESSION   DASYJA010000173 DASYJA010000000
VERSION     DASYJA010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2569437"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2136)
                     /gene="pheT"
                     /locus_tag="VGO02_03695"
     CDS             complement(<1..2136)
                     /gene="pheT"
                     /locus_tag="VGO02_03695"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEV7476824.1"
                     /translation="MNVPESWLRSFCNPKISGQALADKLTMAGVEVEAYQPVGAKLEG
                     EVVVAEVLSVEKHPNADKLTVCKVKAGKETVQVVCGAPNVRAGMKAVLAKIGTKAVRG
                     IDSHGMLASARELGLSEDHSGVLELPADAKPGADARKVLGLDDHVMTLKLTPNRADCL
                     SVLGVAREVSALTGSHLIHPEVKPVGKKSNEKHPVRISAPEGCGRFAGRVIRSVNAAA
                     PTPGWMKQRLERAGQRPISALVDVTNYVMLELGRPLHIYDLDKLKGPIDVRWGRKGEK
                     VLLLNEQEVEVDPSVLCITDDSGVIGLGGIMGGESTKADGATKNVFLESAFFFPDAIQ
                     GRARRYNFSSDASHRFERGVDFANNVEGIERATRLILELCGGEPGPTVDRTARLPKRK
                     PVRMRAARAQKIIGIPLAAKEIAAVFSRLDFKFKKSGDAFAVTPPSYRFDIEIEEDLI
                     EEVARLHGFERIAAQPPRAAALMRPAPDASRSLHALRESLAAREYREVINYSFVEPGW
                     ERDFAGEANPIRLLNPMNQQAVMRTSLIGSLVDVIRKNHFRKVPRIRVFELGRVFLRD
                     PAAADGPLAVRGVRQPMRIAAAAFGPAFAGAWDTAERAVDYFDVKGDLEAMCAPLVLR
                     FEAAQHPALHPGRSARVLVGEAVGWIGELHPKWLQKYELPQAPILLELDAEPLTRLPT
                     PHPRVPSRFPPVVRDISLDFPQETPVQGVL"
     gene            complement(2133..3134)
                     /gene="pheS"
                     /locus_tag="VGO02_03700"
     CDS             complement(2133..3134)
                     /gene="pheS"
                     /locus_tag="VGO02_03700"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEV7476825.1"
                     /translation="MHDLDGIVARALADFASAADPAALENAKARYLGKGGELSALQGT
                     LKSLPPEERREFGAKFNSAKTRIESALEARRAELADTKLASRLGEEALDVTLPGRRAG
                     RGGVHPVIRTWQRIEEIWRSIGFDVADGPEIETDWYNFTALNNPENHPARSMQDTFYV
                     DLKDKDGLPLLLRTHTSPMQVRYARMHKPPIKVIAPGRTYRVDSDATHSPMFHQVEGL
                     WIDEDISFADLKGVYTDFLRRFFEIDDLEVRFRPSFFPFTEPSAEIDMKLKGRWLEIS
                     GSGQVHPQVVRNFGLDPERYIGFAFGSGLERLTMLRHGIEDLRLFFDGDLRFLEQFS"
     gene            complement(3135..3494)
                     /gene="rplT"
                     /locus_tag="VGO02_03705"
     CDS             complement(3135..3494)
                     /gene="rplT"
                     /locus_tag="VGO02_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEV7476826.1"
                     /translation="MPRVKRGVTARARHKKVLTQAKGFRGRRGNVFRIAKEAVMKAGQ
                     YAYRDRRNRKRTMRALWIARINAAVRELGMNYSEFMSGLKRANIEIDRKVLADLAVQD
                     KPAFAKIAGQVKASLAH"
     gene            complement(3505..3705)
                     /gene="rpmI"
                     /locus_tag="VGO02_03710"
     CDS             complement(3505..3705)
                     /gene="rpmI"
                     /locus_tag="VGO02_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEV7476827.1"
                     /translation="MPKMKTKKGAAKRFKVRGSGSIKRASAYMRHILTKKSTKRKRQL
                     RRQNAVDSTNTRAIRAMLPYSS"
     gene            complement(3793..4422)
                     /gene="infC"
                     /locus_tag="VGO02_03715"
     CDS             complement(3793..4422)
                     /gene="infC"
                     /locus_tag="VGO02_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV7476828.1"
                     /translation="MTVDRSQRINREITAPEVRLVAENGDQLGIKPTVEALRLAEEQN
                     VDLVEIAPLANPPVCKLMDYGKFRYREQKKAHEAKLKQKQIQVKEIKFRPGTDEGDYK
                     IKLGKLIEFLEEGDKAKVTLRFRGREMAHKEFGERLLQRVQKDLDAVGLVEQFPRLEG
                     RQMVMVLAPKKKAPPPKSTKPKVEKTEKAEKKPKAEKTEKTVAAKPAAA"
     gene            complement(4454..6367)
                     /gene="thrS"
                     /locus_tag="VGO02_03720"
     CDS             complement(4454..6367)
                     /gene="thrS"
                     /locus_tag="VGO02_03720"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEV7476829.1"
                     /translation="MPNIRLPDGSSKSFPNPVTAAEIAASIGAGLARAALAAKVNGKL
                     VDLSHRIESDADLAIVTERDPEGVEILRHSTAHLLAQAVKELFPDAQVTIGPVIENGF
                     YYDFSYKRPFTPDDLSQIEKRMAEIAKKDLPVQRKEMKRDAAVAFFNSMGEKYKAEII
                     GAIPANEPISLYGQGNWIDLCRGPHVPSTGKLKVFKLMRVAGAYWRGDSKNEMLQRIY
                     GTAWATQEEQDAYLKMLEEAEKRDHRRLGTQLDLFHMQEEAPGLVFWHPKGWTLWQQV
                     EQYMRRIYRDNGYQEVRAPQILDRGLWEKTGHWQNFKENMFTTSSENRDYAVKPMNCP
                     GHILIYNKGVRSYRELPLRYGEFGSCHRNEPSGALHGLMRVRGFVQDDGHIFCTEEQI
                     LPECEAFSTLLQRVYRDFDFTQIIYKVATRPKNRIGSDEAWDRAEKALMQSLEKSKIP
                     FTLSPGEGAFYGPKIEYSLKDGIGRVWQCGTMQVDFSMPGRLGAEYVGEDNARHTPVM
                     LHRAIVGSMERFLGILIEHHAGAFPVWLAPVQVAVLSITEKQAGYADSVKNKLLNQGF
                     RAISDLRNEKISYKIREHSLQKLPYQLVVGDKEMQADTVAVRTRGGEDLGAIRFDEFA
                     ARLLAEANAKVHG"
BASE COUNT          967 a         2110 c         2077 g         1271 t
ORIGIN      
        1 caaaaccccc tgaaccgggg tctcctgggg gaaatccagg gagatatccc gcaccaccgg
       61 gggaaaccgg gagggcaccc gggggtgggg ggtgggcagc cgggtgaggg gctcggcgtc
      121 cagctcgagg aggatgggcg cctggggaag ctcgtatttc tgcagccatt tggggtgcag
      181 ctcgccaatc cagccaaccg cctcgcccac cagcacgcgc gccgagcggc cgggatgcaa
      241 cgccgggtgc tgcgccgcct cgaagcgcag cacgagcgga gcgcacatcg cctcgaggtc
      301 gcctttgacg tcgaagtagt cgaccgcacg ctccgccgta tcccacgcac cggcgaacgc
      361 cgggccgaac gccgccgccg cgatgcgcat cggctgacgc acgccgcgca ccgcgagcgg
      421 gccgtctgcc gccgccgggt cgcgcaggaa cacgcgcccg agctcgaaga cgcggatgcg
      481 ggggactttt ctgaagtgat ttttcctgat cacgtccacc agcgagccga tcagcgaagt
      541 gcgcatcacc gcctgctggt tcatcggatt gagcagccgg atggggttcg cttcgccggc
      601 gaaatcgcgc tcccagcccg gctcgacgaa gctgtagttg atcacctcgc ggtactcgcg
      661 cgcggcgagg ctttcgcgca gcgcgtgcag cgagcggctg gcgtcgggcg ccggccgcat
      721 gagcgccgcg gcgcgcggcg gctgcgccgc gatgcgctcg aagccgtgca ggcgcgccac
      781 ttcctcgatc aggtcctctt cgatctcgat atcgaaacgg tatgagggag gcgtgacggc
      841 aaaggcgtcg ccgcttttct tgaatttgaa atcgagcctg gagaaaacag cagcgatctc
      901 cttcgccgcc agcggaatgc cgatgatctt ctgcgcgcgc gcggcgcgca tgcgcaccgg
      961 cttcctcttc ggcaggcgcg ccgtcctgtc caccgtcggc ccgggctcgc cgccgcacag
     1021 ctcgaggatc aggcgcgtgg cgcgctcgat gccctcgacg ttgttggcga agtcgacgcc
     1081 gcgctcgaag cggtgcgagg cgtcgctcga gaagttgtag cgccgcgccc gcccctgtat
     1141 cgcatcgggg aagaagaagg ccgactcgag gaacacgttc ttcgtcgcgc cgtcggcctt
     1201 ggtgctctcc ccgcccatga tgccgcccag gccgatcacg cccgagtcgt cggtgatgca
     1261 cagcaccgag gggtcgacct cgacctcctg ctcgttgagc agcagcacct tctcgccctt
     1321 gcgtccccag cgcacgtcga tcggcccctt cagcttgtcc aggtcgtaga tgtgcagcgg
     1381 gcgaccgagc tcgagcatga cgtagttggt gacgtcgacc agcgccgaga tcggccgctg
     1441 gccggcgcgc tccaggcgct gcttcatcca gcccggcgtg ggcgccgcgg cgttcacgct
     1501 gcggatcacg cgcccggcga aacgtccgca gccttcgggc gcggagatgc ggaccggatg
     1561 cttttcattg ctctttttac cgaccggttt cacttcagga tgaatcaaat gcgagccggt
     1621 caacgccgat acctcgcgtg ccacgccgag caccgagagg caatccgcgc ggttcggggt
     1681 cagcttgagc gtcatcacgt ggtcgtcgag gccgagcact ttgcgcgcgt cggcaccagg
     1741 cttggcatcg gccggcagct cgagcacgcc ggaatggtct tcggacaagc cgagctcgcg
     1801 cgccgaggcg agcatgccgt ggctgtcgat gccgcgtacc gcttttgttc ctatcttcgc
     1861 cagcacggcc ttcatgccgg cgcgcacgtt cggcgcccca cataccacct gtacggtctc
     1921 cttgccggcc ttcactttgc agacggtcag cttgtcggcg ttcgggtgct tctcgaccga
     1981 aaggacctcg gccacgacga cctcgccttc gagcttggcg cccaccggct ggtacgcctc
     2041 gacctcgacg ccggccatcg tcaatttgtc ggcgagcgcc tgtcccgaga tcttcgggtt
     2101 gcagaagctg cgcagccagc tctcggggac gttcacgaga actgctccag gaagcgcagg
     2161 tcgccgtcga agaacaggcg caggtcctcg atgccatggc gcagcatggt caggcgctcg
     2221 aggcccgagc cgaaggcgaa gccgatgtag cgctccgggt cgaggccgaa gttgcgcacc
     2281 acctgcgggt gcacctggcc cgagccggag atctcgagcc agcggccttt caatttcatg
     2341 tcgatctcgg ccgagggctc ggtgaagggg aagaacgacg gccggaagcg cacctcgagg
     2401 tcgtcgatct cgaagaagcg gcgcaggaaa tcggtgtaga cgcccttcag gtcggcgaag
     2461 ctgatgtcct cgtcgatcca caggccctcg acctggtgga acatcggcga gtgcgtcgcg
     2521 tcggagtcga cgcggtaggt gcggcccggg gcgatgacct tgatcggcgg cttgtgcatg
     2581 cgcgcgtagc gcacctgcat cgggctggtg tgcgtgcgca gcaggagcgg caggccgtcc
     2641 ttgtccttga ggtcgacgta gaacgtgtcc tgcatcgagc gcgccgggtg gttctccggg
     2701 ttgttcagcg ccgtgaagtt gtaccagtcg gtctcgatct cgggcccgtc ggcgacgtcg
     2761 aagccgatcg agcgccagat ctcctcgatg cgctgccagg tgcggatcac cgggtgcacg
     2821 ccgccgcggc cggcgcggcg cccggggagc gtgacgtcga gcgcctcctc gccgaggcgc
     2881 gaggcgagct tcgtatcggc gagctcggcg cgccgcgctt cgagtgcgct ttctatccgg
     2941 gttttcgccg aattgaactt tgcgccgaac tccctgcgtt cctcgggagg aagcgacttg
     3001 agcgtgcctt gcagcgccga aagctcgcca ccttttccta ggtagcgagc tttcgcgttt
     3061 tcgagcgcag ccgggtcagc cgccgacgca aagtcggcga gcgcccgcgc gacgattccg
     3121 tctaaatcgt gcaatcaatg cgcgaggctg gctttgacct ggccggcgat cttggcgaac
     3181 gccggcttgt cctgcaccgc gagatcggcc agcaccttgc ggtcgatctc gatgttcgcg
     3241 cgcttgaggc ccgacatgaa ctcgctgtag ttcatcccca gctcgcgcac tgcggcgttg
     3301 atgcgcgcga tccacagcgc gcgcatggtg cgtttcctgt tgcggcggtc gcggtaggcg
     3361 tactgccccg ccttcatcac cgcctctttg gcgatgcgga acacgttgcc gcggcggccg
     3421 cggaaaccct tggcctgggt caggaccttc ttgtggcgcg cgcgcgccgt gactccacgc
     3481 ttgactcttg gcatggcgcg ctccttagga cgagtaggga agcatcgcgc ggatggcgcg
     3541 cgtgttggtc gagtccaccg cgttctggcg gcggagctgg cgcttccgct tggtgctctt
     3601 cttggtaagg atgtggcgca tgtaggcgct ggcgcgcttg atgctgccgc tgccgcggac
     3661 cttgaaacgc ttggccgcgc ccttcttcgt cttcatctta ggcatgactg cttccttctc
     3721 tcttctagat tgacgccggg cggcccactc cgtggacgct tgatgcctcg gctatcactt
     3781 gtgtcccgtc gcttatgcgg cggcgggctt tgctgcaacc gtcttctcgg tcttctcggc
     3841 cttgggcttc ttctccgcct tctccgtctt ctccaccttg ggcttggtcg acttcggcgg
     3901 cggcgccttc ttcttcggcg ccagcaccat caccatctgc ctgccttcca gccgggggaa
     3961 ctgctcgacc agcccgactg cgtccagatc cttctgcacc cgctgcagca gccgctcgcc
     4021 gaactccttg tgcgccatct cgcggccgcg gaagcgcagc gtcactttcg ccttgtcgcc
     4081 ttcctcgagg aactcgatca gcttgcccag cttgatcttg tagtcgcctt cgtcggtgcc
     4141 ggggcggaac ttgatctcct tcacctggat ctgcttctgc ttgagcttcg cctcgtgcgc
     4201 cttcttctgc tcgcggtagc ggaacttgcc gtagtccatc agcttgcaga ccggcgggtt
     4261 cgcgagcggc gcgatctcca ccaggtccac gttctgctcc tcggcgaggc gcagggcctc
     4321 gaccgtcggc ttgatgccga gctggtcacc gttttccgct accaggcgca cctccggggc
     4381 ggtgatttcc cggttgatgc gctgcgatct gtcgactgta atgagtgaaa agctcctctg
     4441 accgttaaaa aatttagccg tgcaccttcg cgttcgcctc ggccaggagg cgtgcggcga
     4501 attcgtcgaa gcgtatggct ccgaggtctt cgccgccgcg cgtgcgcacg gccaccgtgt
     4561 cggcttgcat ctctttgtcc ccgaccacca gctggtacgg gagtttttgc aagctatgtt
     4621 cccgaatctt ataggagatt ttctcgtttc gcaaatccga aatggctcta aacccctgat
     4681 tcagcaattt atttttcacc gagtcggcgt agccggcctg tttctccgtg atgctcagga
     4741 cggcgacctg gaccggcgcg agccacaccg ggaacgcgcc cgcgtggtgc tcgatcagga
     4801 tgccgaggaa gcgctccatc gagccgacga tcgcgcggtg cagcatcacc ggcgtatggc
     4861 gcgcgttgtc ctcgccgacg tactccgcgc ccagcctgcc gggcatgctg aagtcgacct
     4921 gcatcgtgcc gcattgccag acgcggccga tgccgtcctt gagcgagtac tcgatcttcg
     4981 gtccgtaaaa ggcgccttcg cccggcgaca gggtgaaagg aatctttgat ttttccaaag
     5041 attgcatcaa agccttttcg gcgcgatccc aggcttcatc cgagccgatg cggtttttcg
     5101 gccgcgtcgc caccttgtag atgatttgcg tgaagtcgaa atcccggtag accctctgca
     5161 gcagcgtgct gaacgcctcg cactcgggaa ggatctgctc ctcggtgcag aagatgtggc
     5221 cgtcgtcctg cacgaagccg cgcacccgca tcaatccatg cagcgcgccg gagggctcgt
     5281 tgcggtggca ggagccgaac tcgccgtagc gcagcggcag ctcgcggtag ctgcgcacgc
     5341 ccttgttgta gatcaggatg tgcccggggc agttcatcgg cttcaccgcg tagtcgcggt
     5401 tctccgacga agtggtgaac atgttttcct tgaagttctg ccagtgcccg gtcttctccc
     5461 agaggccgcg gtcgaggatc tgcggcgccc gcacctcctg gtagccgttg tcgcggtata
     5521 tgcggcgcat gtactgctcg acctgctgcc acagcgtcca gcccttcggg tgccagaaga
     5581 ccagccccgg cgcctcctcc tgcatgtgga acaggtcgag ctgcgtgccg aggcgccggt
     5641 ggtcgcgctt ctcggcctcc tcgagcatct tcaggtacgc gtcctgctcc tcctgggtcg
     5701 cccaggcggt accgtagatg cgctggagca tctcgttctt cgaatcgccg cgccagtagg
     5761 cgccggcgac gcgcatcagc ttgaacacct tgagcttgcc ggtcgatggc acgtgcggcc
     5821 cgcggcacag gtcgatccag ttgccctggc cgtaaagcga gatcggttcg ttcgccggga
     5881 tcgcgccaat gatctcggcc ttgtactttt cgcccatcga gttgaagaac gccaccgcag
     5941 cgtcgcgctt catttccttt ctctgcaccg ggagatcctt tttcgcgatc tccgccattc
     6001 ttttttctat ttgagaaagg tcgtcgggcg tgaaaggccg cttgtacgag aagtcgtagt
     6061 agaacccgtt ctcgatcacc gggccgatgg tgacctgggc gtcgggaaag agctccttca
     6121 ccgcttgcgc cagcaggtgc gcggtggagt ggcgcaggat ctcgacgccc tcgggatcgc
     6181 gctccgtgac gatggcgagg tcggcgtcgg actcgatgcg atgcgacagg tcgaccagct
     6241 tgccgttcac tttcgccgcc agcgcggcgc gcgcgaggcc ggcgccgatg gacgctgcga
     6301 tttcggcggc ggtgacgggg ttcggaaacg acttcgacga gccgtcggga agacggatgt
     6361 tgggcattcg cgctggagct tgcatactag catggtaaaa agcaatcttc tggaccgatg
     6421 aatct
//
LOCUS       DASYJA010000174         1847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3205519, whole genome shotgun sequence.
ACCESSION   DASYJA010000174 DASYJA010000000
VERSION     DASYJA010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3205519"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /gene="ruvC"
                     /locus_tag="VGO02_03725"
     CDS             complement(<1..324)
                     /gene="ruvC"
                     /locus_tag="VGO02_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEV7476830.1"
                     /translation="MKGPDYQRILGIDPGLRITGFGVIEKNGSRLQYVASGCVRSGSG
                     ELSSRLKTILEGLAEVIAAHSPGEMAIEKVFVNVNPQSTLALGQARGTAICAAVLAGL
                     PVAEYT"
     gene            complement(321..1043)
                     /locus_tag="VGO02_03730"
     CDS             complement(321..1043)
                     /locus_tag="VGO02_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV7476831.1"
                     /translation="MSGHSKWANIKHRKAAADAKKGKVFTRLIKEITVAARAGGGDPD
                     SNPRLRLSLEKAREANMTKDSVQNAVKRGTGQLEGVSYEEARYEGYGVAGAAVMLDCL
                     TDNRTRTVAEVRHAFSKNGGNLGSDGSVAYLFKHVGQFVFAPGTSEEKLMDAALEAGA
                     DDVVANEDGSVEVLSAPNDFHAVKAALEKAGFKPELAEVTMKPTNENLLSGGDAQRMR
                     HLLDALEALDDVQNVYTTAVID"
     gene            1060..>1847
                     /locus_tag="VGO02_03735"
     CDS             1060..>1847
                     /locus_tag="VGO02_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEV7476832.1"
                     /translation="MASPLLVAKNDKIELCLLPALANRHGLITGATGTGKTVTLQTLA
                     HGLSAIGVPVFMADVKGDLSGLAKPGGGNQKILERFKSFKIDPGFTACPVAFWDVFGK
                     GGHPVRASVSHMGPLLLGRMLALNDTQEGVLQLVFKIADDNGLLLLDLKDLRAMLQHV
                     GDNAAQFKTEYGNVSSATIGIIQRGLLALESQGAAQFFGEPMLDIADLMQVREGKGVV
                     NVLAADKLMSSPKLYATFLLWMLAELFERLPEVGDPEKPKLVFF"
BASE COUNT          283 a          637 c          583 g          344 t
ORIGIN      
        1 agtgtactcg gcgaccggca gcccggcgag caccgccgcg cagatggcgg tgccgcgcgc
       61 ctggccgagc gcgagcgtcg actgcgggtt gacgttgacg aacacctttt cgatcgccat
      121 ttctccgggc gaatgcgccg cgatcacttc ggcgaggcct tccaggatgg tcttgagccg
      181 cgaggaaagc tcgccgctgc cggagcgcac gcagccgctc gccacgtact gcagccggct
      241 gccgttcttc tcgatcacgc cgaatccggt gatcctcagg cctggatcga tgccgagaat
      301 tcgctggtaa tccggacctt tcagtcgatc accgccgtgg tgtacacgtt ctgcacgtcg
      361 tcgagcgcct cgagcgcgtc gagcaggtgg cgcatgcgct gggcgtcgcc gccggaaagc
      421 aggttctcgt tggtcggctt catggtgacc tcggcgagct cgggcttgaa gccggccttc
      481 tccagcgccg ctttcacggc gtggaaatcg ttcggcgcgc tgagcacctc gaccgagccg
      541 tcctcgttcg ccaccacgtc gtcggcgccg gcctcgagcg ccgcatccat cagcttctct
      601 tcgctcgtgc cgggtgcgaa cacgaactgc ccgacgtgct tgaagaggta ggcgaccgag
      661 ccgtcggagc cgagattgcc gccgttcttg ctgaaggcgt ggcgcacctc ggccaccgtg
      721 cgcgtgcggt tgtcggtgag gcagtcgagc atcaccgcag cgcccgctac gccatagcct
      781 tcgtagcgcg cctcctcgta gctcacgccc tcgagctgcc cggtgccgcg cttgaccgcg
      841 ttctgcacgc tgtccttcgt catgttcgct tcgcgagcct tctcgagcga aagccgcagg
      901 cgcgggttgc tgtccggatc gccgccgccg gccctcgccg cgacggtgat ctccttgatc
      961 agccgcgtga acaccttgcc cttcttcgca tccgcagcgg ccttgcggtg cttgatgttc
     1021 gcccatttgc tgtgacccga catagtatga ttttacgtca tggcctcgcc gctcctggtc
     1081 gcgaagaacg acaagatcga gctttgtctt ttgccggcac tcgcgaaccg ccacggcctc
     1141 atcaccggcg ccaccggcac cggcaagacg gtcacgctgc agacgctcgc ccacggcctg
     1201 tccgccatcg gcgtgccggt gttcatggcc gacgtcaagg gcgatctgtc gggcctggcc
     1261 aagccgggcg gcggcaacca gaagattctg gaaagattca aatctttcaa aatcgacccc
     1321 gggttcaccg cctgcccggt cgccttctgg gatgtattcg gcaagggcgg ccacccggtg
     1381 cgcgccagcg tgtcgcacat gggcccgctg ctgctcggcc gcatgctcgc cctcaacgac
     1441 acgcaggaag gcgtcctgca gctggtcttc aagatcgccg acgacaacgg cctgctgctg
     1501 ctcgacctga aggacctgcg cgccatgctg cagcacgtcg gcgacaacgc ggcgcagttc
     1561 aagaccgagt acggcaatgt ctcgagcgcc acgatcggca ttatccagcg tggcctgctc
     1621 gcgctcgaga gccagggcgc ggcgcagttc ttcggcgagc cgatgctcga catcgccgac
     1681 ctgatgcagg tgcgcgaagg caaaggcgtg gtaaacgtgc tcgccgccga caagctcatg
     1741 agctcgccga agctctacgc gaccttcctg ctgtggatgc tggcggagct cttcgagcgc
     1801 ctgccggagg tcggcgaccc cgagaagccg aagctggtct tcttctt
//
LOCUS       DASYJA010000175         5049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_390489, whole genome shotgun sequence.
ACCESSION   DASYJA010000175 DASYJA010000000
VERSION     DASYJA010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390489"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /gene="ppsA"
                     /locus_tag="VGO02_03740"
     CDS             <1..1363
                     /gene="ppsA"
                     /locus_tag="VGO02_03740"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEV7476833.1"
                     /translation="RYALKGSSRVLVEGRAIGNKIGSGTVRIVPDASHMDKVKPGDVL
                     VTDMTDPNWEPVMKVAAAIVTNRGGRTCHAAIIARELGIPAVVGCGDATRKLKDGQAV
                     TVSCAEGDTGNVYDGLIPFEVSSRERGEMPKVAVKIAMNVGNPELAFEFAQLPNAGVG
                     LARLEFIVNGIGVHPKACLEYPGLPSSLKTKLEEKSRGYADPRTFFREKLVEGVATIA
                     AAFWPKPVIVRLSDFKSNEYRKLLGGERYEPEEENPMLGFRGAARYIAASFKECFELE
                     CAALKKVREELGLTNVELMVPFVRTLGEAKQVLSLLSKNGLEKGKGGLKVVMMCELPS
                     NAILADQFLELFDGFSIGSNDLTQLTLGLDRDSGLVAEGFDERDPAVKAMLAMAIRAC
                     RKAGKYVGICGQGPSDHPDLARWLMEQGIDSLSLNPDTVVSTWMALAEAPPPDTEKKS
                     AAA"
     gene            complement(1349..1792)
                     /gene="smpB"
                     /locus_tag="VGO02_03745"
     CDS             complement(1349..1792)
                     /gene="smpB"
                     /locus_tag="VGO02_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEV7476834.1"
                     /translation="MSIVHNRKAGHDYFIEERYEAGIVLDGWEVKSIRAGRAQIADGY
                     VIVKGDELFLIGAHVSPLPTVSTHFIPDPTRSRKLLLHAEEIRRLIGKVEQRGYTLVP
                     LDLHYKKGRIKLEVGLARGKLQHDKRADKKEKEWNREKQRLLRSR"
     gene            1825..2268
                     /locus_tag="VGO02_03750"
     CDS             1825..2268
                     /locus_tag="VGO02_03750"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEV7476835.1"
                     /translation="MKKVTRSAIVEHSADEMYRLVEDIESYPAFLPWCVAARVHERGA
                     ASTRATLTVGMPGLRQSFTTRNDNRPGQSIDMHLIDGPFKTFSGGWRFHTLSPSACRI
                     EFSLRYEFSSRMLARLLEPLFHGIANSMVDAFTRCADERYGRAAG"
     gene            2249..2524
                     /locus_tag="VGO02_03755"
     CDS             2249..2524
                     /locus_tag="VGO02_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEV7476836.1"
                     /translation="MAAQPADIEVEVVYALSRREQSIRLIVPEGATVREVLEQSGLLP
                     QVRGKVGIFGKVVSPNKAVADGDRIEIYRPLPADPKEARRRRAARRR"
     gene            complement(2532..3008)
                     /locus_tag="VGO02_03760"
     CDS             complement(2532..3008)
                     /locus_tag="VGO02_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEV7476837.1"
                     /translation="MNRLLGFALAMGLCFAAAAQQYRWVDQDGRVQYGDTPPPGVKAT
                     RMRGSGAAAPVAPAAAGAEGKPAAKGPLSPAEQEAAFRQRQQEAQKQQETDAQAARDA
                     AQKKDQCARAQDYLRSLEGGQRIARTDSKGERYFLDEAQTAQELATARQGIQQNCN"
     gene            3046..4503
                     /gene="guaB"
                     /locus_tag="VGO02_03765"
     CDS             3046..4503
                     /gene="guaB"
                     /locus_tag="VGO02_03765"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV7476838.1"
                     /translation="MRVIQKALTFDDVLLLPAHSRVLPREVSLKTRLTRKLELNIPLV
                     SAAMDTVTEARLAIAIAQEGGIGIIHKNLTPAAQAAEVAKVKRFESGVLRDPMTVSPE
                     MTVREVLALQREHKISGFPVVKGGKVVGIVTNRDLRFETQLDQPVKAIMTPQRKLVTV
                     REGASREEARELMHRNRLERVLVVGEEFRLMGLVTVKDILKETEHPDASKDAQGKLRV
                     GAAVGVGEGTEERVAALVEAGVDVLVVDTAHGHAQGVLDRVKWVKDNFKVEVIGGNVG
                     TGDGARALVDHGADGVKVGIGPGSICTTRIVAGVGVPQISAIQNVAEALAKSGVPCIA
                     DGGVRYSGDIAKALAAGAHSVMLGSLFAGTEESPGDIELFQGRSYKAYRGMGSLGAMQ
                     KGAADRYFQDSEMNVDKLVPEGVEGRVPYKGSAVSLMQQLVGGVRASMGYTGCATIDE
                     LRTRTQFVEITSAGIRESHVHDVQITKEAPNYRVE"
     gene            4460..>5049
                     /gene="guaA"
                     /locus_tag="VGO02_03770"
     CDS             4460..>5049
                     /gene="guaA"
                     /locus_tag="VGO02_03770"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003266327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEV7476839.1"
                     /translation="MSRSPRRPRITGSSSHQKILILDFGAQYTQLIARRVREAKVYCE
                     IHPHDVSDEFVRGFGAQGVILSGSHMSAYEESTGKAPGAVFDLGVPVLGICYGMQTMA
                     QQLGGRVEAGKVREFGYAEVRARGHTRLLNGIEDFHTPDGHGMLKVWMSHGDKVVDMP
                     AGFKLMASTDACPVAGMADEARRFYAVQFHPEVTHTR"
BASE COUNT          830 a         1686 c         1736 g          797 t
ORIGIN      
        1 gcgctacgcc ctgaagggca gctcgcgcgt gctggtggaa gggcgcgcca tcggcaacaa
       61 gatcggcagc ggcacggtgc gcatcgtccc cgacgcctcg cacatggaca aggtgaagcc
      121 gggcgacgtc ctggtcaccg atatgaccga tcccaactgg gagccggtga tgaaggtggc
      181 ggcggcgatc gtcaccaacc gcggcgggcg cacctgccac gccgcgatca tcgcccgcga
      241 gctcggcatc ccggcggtgg tcggctgcgg cgacgccacg cgcaaattga aggacggcca
      301 ggcggtgacc gtatcctgcg ccgagggcga caccggcaac gtctacgacg gcctgatccc
      361 cttcgaggtg tcgagccgcg agcgcggcga gatgccgaag gtcgcggtga aaatcgccat
      421 gaacgtcggc aacccggagc tggccttcga gttcgcccag ctgcccaacg ccggcgtggg
      481 cctcgcgcgg ctggagttca tcgtcaacgg catcggcgtg cacccgaagg cctgcctgga
      541 atatccgggc ttaccttcct ctttaaaaac gaaactggag gaaaaatccc gaggctatgc
      601 cgaccctcgc acgttcttcc gcgagaagct ggtcgagggc gtggcgacca tcgccgccgc
      661 gttctggccc aagccggtga tcgtgcgcct gtccgacttc aagtcgaacg agtaccgcaa
      721 gctgctcggc ggcgagcgct acgagcccga ggaggagaac ccgatgctcg gcttccgcgg
      781 cgccgcgcgc tatatcgcgg cgagcttcaa ggaatgcttc gagctcgagt gcgcggcgct
      841 gaagaaggtg cgcgaggagc tcggcctgac caatgtcgag ctgatggtgc ctttcgtcag
      901 gacgctcggt gaagcaaagc aggtcctttc ccttctttca aaaaatggtc tggaaaaggg
      961 caagggcggt ctcaaggtcg tcatgatgtg cgagctgccc tcgaacgcca tcctcgccga
     1021 ccagttcctc gagctgttcg acggcttctc gatcggctcc aacgacctga cccagctcac
     1081 gctcggcctg gaccgcgact cgggcctggt cgccgagggc ttcgacgagc gcgacccggc
     1141 ggtgaaggcg atgctcgcca tggcgatccg cgcctgccgc aaggcgggca agtacgtcgg
     1201 catctgcggc caggggccgt cggaccatcc ggacctggcg cgctggctga tggagcaggg
     1261 gatcgacagc ctctcgctca accccgacac ggtggtctcg acctggatgg cgctggccga
     1321 agccccgccc cccgataccg agaaaaaatc agcggctgcg tagtaggcgc tgcttctcgc
     1381 ggttccactc cttctccttc ttgtcggcgc gcttgtcgtg ctgcagcttg ccgcgggcca
     1441 ggccgacctc cagcttgatg cggcccttct tgtagtgcag atcgagcggc accagcgtgt
     1501 agccgcgctg ctcgaccttg ccgatcaggc gcctgatttc ctccgcgtgc agcaaaagct
     1561 tgcggctgcg cgtcgggtcg ggtatgaagt gcgtggacac cgtcggcagg gggctgacgt
     1621 gcgcgccgat caggaacagc tcatcgcctt tcacgatcac gtacccgtcg gcgatctgcg
     1681 cgcgcccggc gcggatggac ttgacctccc agccgtcgag cacgatgccg gcttcgtagc
     1741 gctcctcgat gaagtagtcg tggccggctt tgcggttgtg gacgatggac atattggaaa
     1801 tcgggctctg accccgattt tcccatgaag aaggtgacgc ggtcggcgat cgtcgagcac
     1861 agcgccgacg agatgtaccg gctggtcgag gacatcgagt cctatccggc gttcctgcca
     1921 tggtgcgtgg cggcgcgcgt gcacgagcgc ggcgcggcat cgacgcgcgc caccctcacc
     1981 gtcggcatgc cggggctgcg ccagtcgttc accacgcgca acgacaaccg cccgggccag
     2041 tcgatcgaca tgcacctgat cgacggcccg ttcaagacct tctccggcgg ctggcgtttc
     2101 cacacgctgt cgccgagcgc ctgccgcatc gagttctcgc tgcgctacga gttctccagc
     2161 cggatgctgg cgcggctgct cgagccgctg ttccacggca tcgccaactc gatggtcgac
     2221 gcgttcaccc ggtgcgccga cgagcgctat ggccgcgcag ccggctgata tcgaggttga
     2281 agtcgtctac gcgctgtccc ggcgcgagca gagcatccgg ctgatcgtgc cggaaggcgc
     2341 cacggtgcgg gaggttctcg aacagtccgg cctgctgccg caggtgcgcg gcaaggtggg
     2401 catcttcggc aaggtggtga gcccgaacaa agcggtcgcc gacggcgacc gcatcgagat
     2461 ttaccgcccg ctgccggcgg atcccaagga agcgcgccgc aggcgcgcgg cgcgcaggcg
     2521 ctgagagcgc ctcagttgca gttctgctgg atgccctggc gggcggtggc gagctcctgg
     2581 gcggtctgag cttcgtcgag gaagtagcgc tcgcccttgg aatcggtccg cgcgatacgc
     2641 tgaccgccct ccaggctgcg caggtagtcc tgcgcccgcg cgcactggtc ttttttctgc
     2701 gcagcgtcgc gcgccgcctg tgcgtccgtc tcctgctgct tctgcgcctc ctgctgccgc
     2761 tggcggaaag ccgcttcctg ctcggcgggg ctgagcggac ccttggccgc gggcttgccc
     2821 tcggcgcccg ctgccgccgg cgcgaccggc gccgctgcac cgctgccgcg catccgcgtc
     2881 gccttcacgc cgggcggcgg cgtgtcgccg tactgcacgc ggccgtcctg gtcgacccag
     2941 cggtactgct gggcggcggc ggcgaaacac agccccatgg cgagggcgaa gcctaaaagt
     3001 cgattcataa catgagagta taatgccgct ttgcgagaag aaaacatgcg ggtgatacag
     3061 aaggcgctga ccttcgacga cgttctcctc cttcccgccc actcgcgcgt cctgccgcgc
     3121 gaagtctccc tgaagacccg cctcacccga aaactcgagc tgaacatccc gctggtctcg
     3181 gccgccatgg acacggtgac cgaggcgcgc ctggcgatcg ccatcgcgca ggagggcggc
     3241 atcggcatca tccacaagaa cctgaccccg gcggcgcagg cggccgaggt cgccaaggtg
     3301 aagcgcttcg agtccggcgt gctgcgcgac ccgatgaccg tgtcgcccga aatgacggtg
     3361 cgcgaggtgc tggcgctgca gcgcgagcac aagatttccg gcttcccggt ggtgaagggc
     3421 ggcaaggtgg tcggcatcgt caccaaccgc gacctgcgct tcgagaccca gctcgaccag
     3481 ccggtaaagg cgatcatgac gccgcagcgc aagctcgtca ccgtgcgcga gggcgcgagc
     3541 cgcgaagagg cgcgcgagct gatgcaccgc aaccgcctgg agcgcgtgct ggtggtcggc
     3601 gaggagttca ggctcatggg cctggtcacg gtgaaggaca tcctgaagga gaccgagcac
     3661 ccggacgcca gcaaggatgc gcagggcaag ctgcgtgtcg gcgccgcagt cggggtgggc
     3721 gagggcaccg aggagcgcgt ggcggcgctg gtcgaggccg gcgtggatgt gctggtggtc
     3781 gataccgcgc atggtcacgc gcagggcgtg ctcgatcgcg tgaagtgggt gaaagacaat
     3841 ttcaaggtcg aggtcatcgg cggcaacgtc ggcacgggcg acggcgcgcg cgcgctggtc
     3901 gaccacggcg ccgacggcgt caaggtcggc atcgggccgg gctcgatctg caccacgcgc
     3961 atcgtcgccg gcgtcggcgt gccgcagatc agcgccatcc agaacgtggc cgaggcgctc
     4021 gccaagtcgg gcgtgccctg catcgccgac ggcggcgtcc gctactcggg cgacatcgcc
     4081 aaggcgctgg cagccggtgc gcacagcgtc atgctggggt cgctcttcgc cggcaccgag
     4141 gagtcgcccg gcgacatcga gctgttccag gggcgctcgt acaaggcgta ccgcggcatg
     4201 ggctcgctcg gcgcgatgca aaaaggcgcc gccgaccgct acttccagga ctcggagatg
     4261 aacgtcgaca agctggtgcc cgagggcgtc gagggccgcg tgccctacaa gggcagcgcc
     4321 gtttccctca tgcagcagct ggtgggcggg gtgcgcgcga gcatgggcta caccggctgc
     4381 gccaccatcg acgagctgcg cacccgcacc cagttcgtcg agatcacctc ggccgggatc
     4441 cgcgagtcgc acgtgcacga tgtccagatc accaaggagg ccccgaatta cagggtcgag
     4501 tagtcatcaa aaaatcctga tcctggattt cggcgcgcag tacacgcagc tgatcgcccg
     4561 ccgggtgcgc gaggcgaagg tctactgcga gatccacccg cacgacgtgt ccgacgagtt
     4621 cgtgcgcggc ttcggcgcgc agggcgtgat cctctccggc agccacatgt cggcctacga
     4681 ggagtcgacc ggcaaggcgc cgggcgcggt cttcgacctc ggcgtcccgg tcctcggcat
     4741 ctgctacggc atgcagacca tggcgcagca gctcggcggc cgcgtcgagg cgggcaaggt
     4801 acgcgaattc ggctacgcgg aggtccgcgc acgcggccac acacggttgt tgaacggcat
     4861 cgaagacttt catacgcccg acggccacgg catgctcaaa gtctggatga gccacggcga
     4921 caaggtggtc gacatgccag cgggcttcaa gctgatggcg tcgaccgacg cctgcccggt
     4981 cgccgggatg gccgacgagg cgcggcgctt ctacgccgtg cagttccacc ccgaagtcac
     5041 gcacacccg
//
LOCUS       DASYJA010000176         2910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2024527, whole genome shotgun sequence.
ACCESSION   DASYJA010000176 DASYJA010000000
VERSION     DASYJA010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2024527"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VGO02_03775"
     CDS             complement(<1..971)
                     /locus_tag="VGO02_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEV7476840.1"
                     /translation="MEREQATKFIYDLLRALVAKKGSDLFITAGFPPAMKIDGKMTPV
                     SQQALAAGHTAMLTRAVMNDKQAAEFEAKKECNFAINPSGIGRFRVNCFVQMGCVGMV
                     LRVITTSIPSFDDLKLPAVLKDVAMTKRGLVIFVGGTGSGKSTSLAAMIGYRNENSHG
                     HIITIEDPVEYVHPHKNCLITQREVGVDTESWEAALKNTLRQAPDVILIGEIRERETM
                     EHAIAFSETGHLCLGTLHANNSNQAMDRIINFFPEERRQQLLMDLSLNLKAVVSQRLI
                     PLKQGKGRAAAVEIMLNSPLISDLIFKGEVHEIKEIMKKSRELGMQT"
     gene            complement(974..2020)
                     /locus_tag="VGO02_03780"
     CDS             complement(974..2020)
                     /locus_tag="VGO02_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEV7476841.1"
                     /translation="MDLTELLAFVVKNKASDLHLSSGLPPMIRVHGDVRRINVPPMEH
                     KEVHGMIYDIMNDGQRKFYEENLECDFSFAVPNLARFRVNAFVQQRGAAAVMRTIPSK
                     ILSLADLKCPKIFEEIADQPRGIVLVTGPTGSGKSTTLAAMVNHKNENEQGHILTIED
                     PIEFVHESKKCLINQREVGPHTLSFSNALRSALREDPDVILVGEMRDLETIRLALSGA
                     ETGHLVFGTLHTSSAAKTIDRIVDVFPAAEKEMVRSMLSESIRAVISQTLLKTKDGQG
                     RTAAHEIMIGTPAIRNLIRENKIAQMYSAIQTGRQIGMQTLDQNLQELVARNQISSAE
                     ARSKAMNKDNFAGT"
     gene            2065..2745
                     /locus_tag="VGO02_03785"
     CDS             2065..2745
                     /locus_tag="VGO02_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV7476842.1"
                     /translation="MAAIGDNLQVVRRRIERAALDAGRRPGSVTLLAVSKTHAAARVM
                     EAAAAGQRAFGENYVQEALEKMDAAPGLEWHLIGPLQSNKTRVAAERFDWVHTVASEK
                     IARRLSEQRPAGLAPLNVLIQVNASEETSKSGVAPAAAAALASAIAPLKNVRLRGLMA
                     IPETGAPRARFHEVNALYERLKNEFVLDTLSMGMSDDMDTAIAEGATMVRIGTAIFGQ
                     RQKHQSAA"
     gene            2742..2873
                     /locus_tag="VGO02_03790"
     CDS             2742..2873
                     /locus_tag="VGO02_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476843.1"
                     /translation="MKIAFLGGGNMASARFMSCGFTASTSVSAPLMASSGAVVTRTG"
BASE COUNT          456 a          904 c          983 g          567 t
ORIGIN      
        1 aaggtctgca tgccgagctc gcgcgacttc ttcatgatct ccttgatctc gtgcacctcg
       61 cccttgaaga tcaggtcgct gatcaatggc gagttgagca tgatttccac cgccgcggcg
      121 cggcccttgc cttgcttcag cgggatcagg cgctgcgaca ccaccgcctt caggttgagc
      181 gacaggtcca tcaggagctg ctggcggcgc tcttccggga agaagttgat gatgcggtcc
      241 atcgcctggt tggagttgtt ggcgtgcagc gtgccgaggc acaggtgccc ggtctcggag
      301 aaggcgatgg cgtgctccat cgtctcgcgc tcgcggatct cgccgatcag gatgacgtcc
      361 ggcgcctggc gcaaggtgtt cttgagcgcc gcctcccagg actcggtgtc gacgccgacc
      421 tcgcgctggg tgatgaggca gttcttgtgc gggtggacgt actcgaccgg gtcctcgatg
      481 gtgatgatgt ggccgtgcga attctcgttg cggtagccga tcatcgcggc gagcgaggtc
      541 gacttgcccg agccggtgcc gccgacgaag atcaccaggc cgcgcttggt catcgccacg
      601 tccttcagca cggcgggcag cttcaggtcg tcgaagctcg ggatgctggt ggtgatcacc
      661 cgcagcacca tgccgacgca gcccatctgc acgaagcagt tgacgcggaa gcggccgatg
      721 ccggaggggt tgatggcgaa gttgcactcc ttcttcgcct cgaactcggc cgcctgcttg
      781 tcgttcatca ccgcgcgggt gagcatcgcg gtgtgcccgg cggcgagcgc ctgctgcgac
      841 accggggtca tcttgccgtc gatcttcatc gccggcggga agccggcggt gatgaagagg
      901 tccgagcctt tcttcgccac cagcgcgcgc agcaggtcgt agatgaattt ggttgcttgc
      961 tctcgttcca tggtcacgtc cccgcgaagt tgtctttgtt catcgccttg ctgcgcgcct
     1021 cggcgctcga gatctggttg cgcgccacca gctcctgcag gttctggtcg agggtctgca
     1081 tgccgatctg gcggccggtc tggatcgccg agtacatctg cgcgatcttg ttctcgcgga
     1141 tcaggttgcg gatcgccggg gtgccgatca tgatctcgtg cgccgcggtg cgcccctggc
     1201 cgtccttggt cttgagcagc gtctgcgaga tgaccgcgcg gatcgattcc gagagcatcg
     1261 agcgcaccat ctccttttcc gccgccggga agacgtcgac gatgcggtcg atggtcttgg
     1321 cggccgacga ggtatgcagc gtgccgaata ccaggtggcc ggtctccgcg cccgacaggg
     1381 cgaggcggat ggtctccagg tcgcgcatct cgccgaccag gatcacgtcg gggtcctcgc
     1441 gcagcgcgct gcgcagcgcg ttggagaagg aaagcgtgtg cgggccgacc tcgcgctggt
     1501 tgatcaggca cttcttcgac tcgtgcacga actcgatcgg gtcctcgatg gtgaggatgt
     1561 ggccctgctc gttctcgttc ttgtggttga ccatcgccgc gagcgtggtc gacttgcccg
     1621 agccggtcgg tccggtcacc agcacgatgc cgcgcggctg gtcggcgatt tcctcgaaga
     1681 tcttcgggca ctttaggtcg gcgagcgaca ggatcttgga ggggatggtc cgcatcaccg
     1741 cggccgcgcc gcgctgctgc acgaaggcgt tgacgcggaa gcgcgccagg ttcggcaccg
     1801 cgaaggagaa gtcgcactcg aggttttcct cgtagaactt cctctgcccg tcgttcatga
     1861 tgtcgtagat catgccgtgc acttccttgt gctccatggg cgggacgttg atgcgccgca
     1921 cgtcgccgtg cacgcggatc atcggcggca ggccggaaga caggtgcagg tcggacgcct
     1981 tgttcttcac cacgaaggcg agcagttcgg tgagatccat tacaatcctc ccagcgagaa
     2041 aatttgccgc tgtttcaggg gattatggct gcaattggcg acaacctgca agttgtaaga
     2101 aggcgcatcg agcgcgccgc gctcgatgct ggccggcgtc ctggatcggt gactttgttg
     2161 gcagtctcca agacccatgc cgccgcccgg gtaatggagg ccgccgccgc cggccagcgt
     2221 gccttcggcg agaactacgt gcaggaggcg ctggagaaaa tggatgccgc gccggggctc
     2281 gaatggcacc tgatcggacc cctgcagagc aacaagaccc gcgttgcagc cgagcgcttc
     2341 gactgggtgc acacggtggc gagtgaaaaa attgcacgcc ggctctccga gcagcggccc
     2401 gcgggtctgg cacccctcaa tgtgctgatc caggtgaacg cctccgagga gacttccaag
     2461 tccggcgttg cgcctgcggc ggccgctgcg ctggcctccg cgatcgcgcc gttgaagaac
     2521 gttcgcctgc gcgggctgat ggcgatcccg gagacgggcg cgccacgtgc ccgctttcat
     2581 gaagtaaatg ccttgtatga aaggctgaag aacgagttcg tgctcgacac cctgtcgatg
     2641 gggatgagcg acgatatgga cacggcgatc gccgaaggcg ccacgatggt gcgcatcggc
     2701 acggccatct tcggccagag acagaaacac cagagcgcgg catgaaaatc gccttcctcg
     2761 gcggcggcaa catggcgagc gcgcgcttca tgtcctgcgg cttcaccgcc agcacgagcg
     2821 tgtcggcgcc cctcatggcg tcgtcgggcg cggtcgtgac gcgcaccggg tagcgcgcgc
     2881 tgagcttctc gcgcgccgcc gggctcatct
//
LOCUS       DASYJA010000177         4235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_482737, whole genome shotgun sequence.
ACCESSION   DASYJA010000177 DASYJA010000000
VERSION     DASYJA010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482737"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VGO02_03795"
     CDS             <1..459
                     /locus_tag="VGO02_03795"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013204975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEV7476844.1"
                     /translation="RRLRLRFIDSDREIEGRCGVKIPVIFEIEGESGFRAREALAIDA
                     LTALEGVVLATGGGAVLAEPNRRHLATRGTVVYLSAKPEDLYERVRHDRNRPLLAGGE
                     PLARLRELHGQRDQLYRAVADIVLETGAQSVQSLARQLLDRLEAEWKASA"
     gene            441..1517
                     /gene="aroB"
                     /locus_tag="VGO02_03800"
     CDS             441..1517
                     /gene="aroB"
                     /locus_tag="VGO02_03800"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEV7476845.1"
                     /translation="MESLRVALGSRSYPIHIGVGLLARADLYRPYLAGGSVAIVTSQT
                     VGSLYLSKVKQGLQGARITEVIVPDGEQAKGWQTLEGVFDTLLAARLGRDGLVVALGG
                     GVVGDLAGFAAAVYQRGIDFIQVPTTLLAQVDSSVGGKTAINHARGKNMIGAFHQPLA
                     VIADVATLDTLPDRELRAGLAEVIKHALALDEAFTRWLEANVEKLLARDRAALTYAVR
                     RSCELKSAIVADDERESGVRALLNFGHTFGHAIEAGAGYGAWLHGEAVAAGMVMASEL
                     SLRIGNIQKGDVVRVRELLRRAGLPVAGPAITPERMLELMAVDKKAAKGRVRFVVLEA
                     VGKAALRGDLPEAAVREAIVAALQ"
     gene            1564..>4235
                     /locus_tag="VGO02_03805"
     CDS             1564..>4235
                     /locus_tag="VGO02_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003273598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HEV7476846.1"
                     /translation="MPEAQGLYHPSNEHDACGVGFVANIKGKKSHSIVEQGLTVLRNL
                     THRGAVGWDPKLSDGAGLLIQIPDKFLREAMARQGVKLPPVGQYGVGMVFLPREPASR
                     RACQYELERAIKDEGQVLLGWRDVPVDNSDLAEPAKKVEPVIRQVFVGRGRGVTVTDA
                     LERKLYIIRKSAGHAIQALKLAHGKEYYVPSMSARTMIFKGMLLAHQVGEYYLDLKDP
                     KVESALAMIHQRFSTNTFPSWDLAHPFRMIAHNGEINTVRGNVNWIRARQGAISSPVL
                     GDDLGKIWPLIYDGQSDSASFDNALELLVMGGYSVAHAMMMMIPEAWENHSLMDPARR
                     AFYEYHAAMMEPWDGPASIAFTDGRQIGATLDRNGLRPSRYIVTDDDLVIMGSECGCL
                     PVPEEKVVKKWRLQPGKMFLVDLEKGRIVDDKELKDTLAGMRPYAEWIERIRIKLDEV
                     ESDKQKPERSSVPLLDRQQAFAYTQEDVKFLMTPMAQAGEEPIGSMGNDSPMAVLSNK
                     DKTLYHYFKQLFAQVTNPPIDPIREELVTSLVSFIGPKPNLLGIDEMNPPLRLEVSQP
                     VLDFFEMEKVRHIERYTQGKFQATELDITYQAAWGNEAIEARLASLCAQAEDAVRSGT
                     SIIIISDRKLNRERVAIPALLALSAIHQHLVAKGLRTSTGLVVETGSAREVHHFALLG
                     GFGAEAVHPYLAMETLLELASQGRLGDLDGKKALKNFVKAMGKGLKKVMSKMGISTYM
                     SYTGAQIFEAVGLSRTLVDKYFTGTASNVGGIGLFEVAEEALRVHRAAFDERDPVLQG
                     ALDAGGEYAWRVRGEEHMWTPDAIAKLQHAARKESYATYKEYAQIINDQSTRHMTFRG
                     LFEFRRENVKPVPLEEVEPAAEIVKRFATGAMSLG"
BASE COUNT          717 a         1426 c         1458 g          634 t
ORIGIN      
        1 cgccgcctca ggctgcgctt catcgattcc gaccgcgaga tcgagggccg ctgcggggtg
       61 aagatccccg tcatcttcga gatcgagggc gagagcgggt ttcgcgcgcg cgaggcgctc
      121 gccatcgacg ccctcaccgc gctcgaaggc gtggtcctcg ccacgggggg cggcgcggtc
      181 ctcgccgagc cgaaccgccg ccacctcgcg acgcgaggca cggtggtgta tctgagcgcg
      241 aagcccgagg atctgtacga gcgcgtgcgg cacgatcgca accggccgct gctcgccggc
      301 ggcgagccgc tggcgcggct gcgcgagctg cacgggcagc gcgatcagct ttaccgcgcc
      361 gtcgccgaca tcgtgctgga gaccggcgcg cagagcgtgc agtcgctggc gcgccagctc
      421 ctcgaccggc tggaggccga gtggaaagcc tccgcgtagc cctcggcagc cgctcctatc
      481 cgatccatat cggcgtcggt ctgctcgcgc gcgccgacct gtaccgcccg taccttgccg
      541 gcggcagcgt cgccatcgtc accagccaga ccgtcgggtc gctctatctt tcgaaagtga
      601 agcaggggct gcagggcgcg cgcatcaccg aggtcatcgt cccggacggc gagcaggcga
      661 agggctggca gacgctggag ggcgtcttcg acaccctgct tgccgcgcgg ctcggccgcg
      721 acggcctggt ggtcgcgctc ggcggcggcg tggtcggcga cctcgcgggc ttcgccgcgg
      781 ccgtgtatca gcgcggcatc gacttcatcc aggtgccgac gacgctgctc gcgcaggtcg
      841 attcctccgt cggcggcaag accgccatca accacgcgcg cggcaagaac atgatcggcg
      901 ccttccacca gccgctggcc gtcatcgccg acgtggcgac gctcgacacg ctgcccgacc
      961 gcgagctgcg cgccggcctc gccgaggtga tcaagcacgc cctggcgctg gacgaagctt
     1021 tcacgcgctg gctcgaggcg aacgtcgaga agctgctggc gcgcgaccgg gcggcgctga
     1081 cttacgcggt gcgccgcagc tgcgagctca agtcggcgat cgtcgccgac gacgagcgcg
     1141 agtcgggcgt tcgggcgctg ctcaatttcg gccacacctt cggccatgcc atcgaagccg
     1201 gcgccgggta cggcgcctgg ctgcacggcg aggcggtggc ggccgggatg gtcatggcga
     1261 gcgagctgtc gttgcgcatc ggaaacatcc agaaaggcga cgtcgtgcgc gtgcgcgagc
     1321 tgctcaggcg cgccgggctg ccggtggcgg gtccggcgat cacgcccgag cgcatgctcg
     1381 agctgatggc ggtggacaag aaggcggcca agggccgggt gcgcttcgtc gtgctcgagg
     1441 cggtcggcaa ggcggcgtta cgcggcgatc ttcccgaggc ggcagtgcgc gaggccattg
     1501 tcgctgcact gcaataaagg tatattcgtc taaggcttat cgattctcag ggtcttggac
     1561 gtgctgccgg aagcgcaagg gctgtatcac ccctccaacg agcacgacgc ttgcggcgtc
     1621 ggctttgtcg cgaacatcaa gggcaagaag tcgcattcca tcgtggagca gggcctcacg
     1681 gtgctgcgca acctcaccca ccgcggcgcg gtcggctggg acccgaagct ctccgacggc
     1741 gccgggctgc tgatccagat cccggacaag ttcctgcgcg aggcgatggc caggcagggt
     1801 gtcaagctgc cgccggtcgg gcagtacggc gtcggcatgg tgttcctgcc gcgcgagccg
     1861 gcctcgcgcc gcgcctgcca gtacgagctc gagcgcgcca tcaaggacga aggccaggtg
     1921 ctgctcggct ggcgcgacgt gccggtggat aacagcgacc tggccgagcc ggcgaagaag
     1981 gtcgagccgg tgatccgcca ggtgttcgtc ggccgcggtc gcggcgtgac ggtcaccgac
     2041 gcgctcgagc gcaagctcta catcatcagg aaaagcgccg gccacgccat ccaggcgctc
     2101 aagctcgccc acggcaagga gtactacgtg ccgtcgatgt cggcgcgcac catgatcttc
     2161 aagggcatgc tgctcgcgca ccaggtaggc gagtactacc tcgacctgaa ggacccgaag
     2221 gtcgagtcgg cgctcgccat gatccaccag cgcttctcga cgaatacgtt cccgtcgtgg
     2281 gacctggcgc atcccttccg catgatcgcg cacaacggcg agatcaacac cgtgcgcggc
     2341 aacgtgaact ggatccgcgc gcggcagggg gcgatttcga gcccggtgct cggcgacgac
     2401 ctcggcaaga tctggccgct gatctacgac gggcagtccg actcggcatc gttcgacaac
     2461 gcgctcgagc tgctggtaat gggcggctac tcggtggcgc acgccatgat gatgatgatc
     2521 ccggaggcct gggagaacca cagtctcatg gacccggcgc gccgcgcgtt ctacgagtac
     2581 cacgcggcga tgatggagcc gtgggacggc ccggcgtcga tcgccttcac cgacggccgc
     2641 cagatcggcg ccacgctcga ccgcaacggc ctgcgcccct cgcgctacat cgtcaccgac
     2701 gacgacctgg tcatcatggg ctcggaatgc ggctgcctgc cggtacccga ggagaaggtg
     2761 gtcaagaagt ggcgcctgca gccgggcaag atgttcctcg tcgacctgga gaaaggccgc
     2821 atcgtcgacg acaaggagct gaaggacacg ctcgccggca tgcgcccgta cgccgaatgg
     2881 atcgagcgca tccgcatcaa gctggatgaa gtggagagcg acaagcagaa gcccgagcgc
     2941 tccagcgtcc cgctgctcga ccgccagcag gccttcgcct acacgcagga ggacgtcaag
     3001 ttcctgatga cgccgatggc gcaggccggc gaggagccga tcggctctat gggcaacgac
     3061 tcgccgatgg cggtgctgtc gaacaaggac aagacgctct accactactt caagcagctc
     3121 ttcgcccagg tcaccaaccc gccgatcgac ccgatccgcg aggagctggt cacttcgctg
     3181 gtttccttta tcggcccgaa gcccaacctg ctcggcatcg acgagatgaa tccgccgctg
     3241 cgcctggagg tgagccagcc ggtgctcgac ttcttcgaga tggagaaggt gcgccacatc
     3301 gagcgctaca cgcagggcaa gttccaggcg accgagctgg acatcaccta ccaagccgcc
     3361 tggggcaacg aggccatcga agcccgcctg gcgtcgctct gcgcccaggc cgaggacgcg
     3421 gtgcgctcgg gcacctcgat catcatcatc tccgaccgca agctgaaccg cgagcgcgtc
     3481 gcgatcccgg cgctgctcgc gctctccgcg atccaccagc acctggtggc gaaggggctg
     3541 cgcacgtcga ctggcctggt ggtggagacg ggctcggcgc gcgaggtgca ccacttcgcg
     3601 ctgctcggcg gcttcggcgc cgaggcggtc catccctacc tcgccatgga gacgctgctc
     3661 gagctcgctt cgcaaggccg gctcggagac ctcgacggga agaaggcgct gaagaacttc
     3721 gtcaaggcga tgggcaaggg gctgaagaag gtgatgtcca agatgggcat ctccacctac
     3781 atgtcctaca ccggcgcgca gatcttcgag gcggtcgggc tgtcgcgcac gctggtcgac
     3841 aaatacttca ccggcaccgc ctcgaacgtc ggcgggatcg ggctgtttga agtcgcagaa
     3901 gaagcgttgc gtgtgcatag agccgccttc gacgagcgcg acccggtgct gcagggggcg
     3961 ctcgacgccg gcggcgagta tgcctggcgc gtgcgcggcg aggagcacat gtggacgccg
     4021 gacgcgatcg ccaagctgca gcacgccgcg cgcaaggagt cctacgcgac ctacaaggaa
     4081 tacgcgcaga tcatcaacga ccagtcgacg cgccacatga ccttccgcgg cctgttcgag
     4141 ttccgccgcg agaatgtgaa gccggtgccg ctggaggaag tcgagcccgc cgccgagatc
     4201 gtcaagcgct tcgccaccgg cgccatgtcg ctcgg
//
LOCUS       DASYJA010000178         6827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1661541, whole genome shotgun sequence.
ACCESSION   DASYJA010000178 DASYJA010000000
VERSION     DASYJA010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6827
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661541"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /gene="rpoD"
                     /locus_tag="VGO02_03810"
     CDS             <1..1083
                     /gene="rpoD"
                     /locus_tag="VGO02_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEV7476847.1"
                     /translation="FKFSTYATWWIRQAITRAIADQGRTIRVPVHMIESINKINRVSR
                     AHFIKVFPGNETNLKWANEEVASGKAYAKALDRFRPAVMEQQEALIHLQKEVGIPIKD
                     LKEIARQMSTGEAKARRAKREMIEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKA
                     VDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQILQE
                     TGQEPDPATLAVKMEMPEEKIRKILKISKEPISMETPIGDDDDSHLGDFIEDQAGLAP
                     SDSALYASLREATKDVLDSLTPREAKVLRMRFGIEMSTDHTLEEVGKQFDVTRERIRQ
                     IEAKALRKLRHPSRSERLKSFLDQEG"
     gene            1144..1220
                     /locus_tag="VGO02_03815"
     tRNA            1144..1220
                     /locus_tag="VGO02_03815"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1178..1180,aa:Ile,seq:cat)
     gene            1238..1819
                     /locus_tag="VGO02_03820"
     CDS             1238..1819
                     /locus_tag="VGO02_03820"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEV7476848.1"
                     /translation="MKFIGDFSIDPQICDRLVELHRACERQGLVKAGRMYNAGSSDAA
                     VDPKKKESLDVAVGDIPEELQNQYGVPLYIRELQRCTHEYLERYRHLQVAKFGVKESP
                     IIQHYRPGGGFKAEHFERSNLATASRMLVWMTFLNDVTDAGGTRFVYQDATFDARRGR
                     TLVWPSDFTHTHVGVVSPSQHKYIITGWFNFVA"
     gene            complement(1816..2883)
                     /locus_tag="VGO02_03825"
     CDS             complement(1816..2883)
                     /locus_tag="VGO02_03825"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7476849.1"
                     /translation="MKIESAEPISLLEARFEAQKIAFGPLIFQAARLLRELGILEALR
                     AAPAGLALEEIVARVDTSGYGVLVLLEAGLAAGLVRAQGSRFLLTTTGACILKDELTR
                     INMDFVQQGCFQAMYYLEDSIRQGEPAGLRKVFGEWDTIYQALPALPEAVRASWLRWD
                     HYYSDAAFAQALPVVFEGSPRALLDVGGNTGKWAIRCAQHSPDVSITILDLPGQVAAA
                     KQNVERLGLQARINAKAIDLLDHTQPFPAGFDAIWMSQFLVCFSEADVLHLLQRAAAA
                     MRPDSILYILDTFWDRQEHEVASFCLQAISLYFTCLANGNSRMYKFSEIAALVEAAGL
                     KIESVSGTLGICSSLLACRRR"
     gene            complement(2922..3314)
                     /locus_tag="VGO02_03830"
     CDS             complement(2922..3314)
                     /locus_tag="VGO02_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476850.1"
                     /translation="MSYPDIDEARGSHAALKEVFAHHRGDWTAGGEELRRVEQLCRDA
                     AAAVDDPQCREEIGIVGDYAAELFARGEHRKWESESMSGAEFLRLQILKALDSFHSRL
                     YSIEAIRRAGDRQLYPFTARARGGERAP"
     gene            complement(3428..4195)
                     /locus_tag="VGO02_03835"
     CDS             complement(3428..4195)
                     /locus_tag="VGO02_03835"
                     /inference="COORDINATES: protein motif:HMM:NF018928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HEV7476851.1"
                     /translation="MPPAPPSIAPPFGYQEVVPLLKTHRLRLPAAGAVPEFLRRANAV
                     PISHTEFQAAAREYAIVFTTGDEGKTFAAVAVLGIAAGENLFFADGAWAAGVYLPAYS
                     RRYPFCMARVTLNQVAQQDRLICVEKAAIDEAAGEAMFDAQGKPAQKWTGIERLLTEY
                     EADLERSREMCAVLADYGLLEPFTMQATLNKEKGGGAMQLTGMQRVNEKNLENLNAAQ
                     LKNLMRKGFLARIYLHLLSLENFARLLERKAARQPAT"
     gene            4234..4309
                     /locus_tag="VGO02_03840"
     tRNA            4234..4309
                     /locus_tag="VGO02_03840"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4267..4269,aa:Ala,seq:cgc)
     gene            4359..5456
                     /locus_tag="VGO02_03845"
     CDS             4359..5456
                     /locus_tag="VGO02_03845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEV7476852.1"
                     /translation="MEAHRLVAAALQRIVDKLDPHEGRYRMLFESSPLPMWVFDAGTL
                     RYLAVNDAAVRLYGYSRDEFLGMTVEDLRPQGEGEGEAFREFLRRESGSMQRGLFHHL
                     KKGGEPIDVETVGHLVRWRGREARLVQINDVTERLRVQTRLRALSQRLLEVQEEERRS
                     LARDLHDDIGQALTALKIQLESLAQSQSGEGEPRRRVDECVETTRHTLERVRQLSLNL
                     RPLQLDDLGLVAALRSHLDRQAGLGGLAPHFDAGEAPPQLDPEIETACFRVAQEAITN
                     VLRHARARNLWVRLFTTGDRLALSVRDDGGGFDPDSARRRAHAGGSLGLAGMEERISI
                     AGGTIELRSRPGQGTVLLATFPIERAGGQGR"
     gene            5453..6100
                     /locus_tag="VGO02_03850"
     CDS             5453..6100
                     /locus_tag="VGO02_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7476853.1"
                     /translation="MMIRVVLADDHALVRAGIRSLLGNMAEVAVVGEASSGEEAMLLA
                     ESERPDVILMDIAMKGISGLEAAAHLRESHPAVRVVILSMHSGEEYVLQALRAGAVGY
                     LLKDAATGELELALRSVMRGESWLSPAVSRQVVEGYVQRVGGDAAPEVLTARQREVLK
                     LVAGGKSTKEIAFLLNLSVKTVETHRAQIMERLGIRDVAGLVRYALRTGLIPPEG"
     gene            6212..6592
                     /locus_tag="VGO02_03855"
     CDS             6212..6592
                     /locus_tag="VGO02_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7476854.1"
                     /translation="MARTRRIAIADDSPAFVSAVSNYVESLPGYELAGTARTSVQALA
                     LVKSQVLDVLLLDLGLPPVRGLEFVRRLKALPGGPAVVAMTLFHTPEAAAEARRAGAD
                     ALVGKEAFVAGLNQALAKLFPTAS"
     gene            6606..>6827
                     /locus_tag="VGO02_03860"
     CDS             6606..>6827
                     /locus_tag="VGO02_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476855.1"
                     /translation="MPRQPLIEERLRATFMMYAALSAANEAILRAKSPQEVFHSVCDI
                     AVQTGGFLLATVFRLDAQAGLLERVTASGP"
BASE COUNT         1173 a         2255 c         2305 g         1094 t
ORIGIN      
        1 ttcaagttct cgacctacgc cacgtggtgg atccgccaag ccatcacgcg cgccatcgcc
       61 gaccagggcc gcacgatccg cgtcccggtg cacatgatcg agtcgatcaa caagatcaac
      121 cgcgtgagcc gcgcgcactt catcaaggtc ttccccggca acgagaccaa cctcaagtgg
      181 gccaacgagg aggtcgcctc cggcaaggcg tacgcgaagg ccctcgaccg cttccgcccg
      241 gcggtgatgg agcagcagga agcgctgatc cacctgcaga aggaagtcgg gatcccgatc
      301 aaggacctga aggagatcgc tcgccagatg tccacgggcg aggcgaaggc gcgccgcgcg
      361 aagcgcgaga tgatcgaggc caacctgcgc ctcgtgatct ccatcgcgaa gaagtacacc
      421 aaccgcggcc tgcaattcct cgacttgatc caggaaggca acatcggcct gatgaaggcc
      481 gtggacaagt tcgaataccg ccgcggctac aaattctcga cgtacgcgac gtggtggatc
      541 cgccaggcca tcacgcgctc gatcgccgac caggcgcgca ccatccgcat cccggtgcac
      601 atgatcgaga ccatcaacaa gatgaaccgc atctcgcgcc agatcctcca ggaaaccggc
      661 caggagccgg atccggcgac gctcgccgtg aagatggaga tgccggaaga gaagatccgc
      721 aagatcctca agatctcgaa agagccgatc tcgatggaaa cgccgatcgg agacgacgac
      781 gattcccacc tgggcgactt catcgaggac caggcgggcc ttgcgccttc cgattccgcg
      841 ctctacgcga gcctgcgcga agcgaccaag gacgtgctcg attccctgac gccgcgcgaa
      901 gcgaaggtgc tgcgcatgcg ctttggcatc gagatgtcga ccgaccacac gctggaagag
      961 gtcggcaagc agttcgacgt cacgcgcgag cgcatacgcc agatcgaggc caaggcgctg
     1021 cgcaagctgc gccacccgtc gcggtccgag cgactaaaaa gcttcctcga tcaagaggga
     1081 taacaagaga aggaaacacg acaagggccg cgaaagcggc ctttttcgtt tataaggcaa
     1141 gaggggccgg tagctcagtt ggtcagagca ggggactcat aatcccttgg tcgttggttc
     1201 aaatccaacc cggcccacca tcacgtaata tcggcgcatg aagttcatcg gcgatttttc
     1261 catcgacccg cagatctgcg atcgcctggt cgagctgcat cgcgcttgcg agcgccaggg
     1321 gctggtcaag gccgggcgca tgtacaacgc gggaagcagc gacgccgccg tcgatcccaa
     1381 gaagaaggag tcgctcgacg ttgcggtcgg cgacatcccc gaggagctgc agaatcaata
     1441 cggcgtgccg ctgtacatca gggagctgca gcgctgcacg cacgagtacc tcgagcggta
     1501 tcgccatctg caggtggcga agttcggcgt gaaggagtcg ccgatcatcc agcactaccg
     1561 cccgggcggc ggcttcaagg ccgagcactt cgagcgcagc aacctcgcta ccgccagccg
     1621 catgctggtg tggatgactt ttctcaacga tgtgaccgac gccggcggaa cgcgattcgt
     1681 ctaccaggac gccacgttcg acgcccgcag gggccgcacg ctggtatggc cgtcggactt
     1741 cacgcacacg cacgtcgggg tcgtctcgcc ctcgcagcac aagtacatca tcacgggctg
     1801 gttcaacttc gtcgcctagc ggcgccggca ggcgagcagc gacgagcaga tgccgagggt
     1861 gccgctcacg ctctcgattt tcaatccggc ggcctcgacc agcgccgcga tctcggagaa
     1921 cttgtacatc cggctgttgc cgttcgcgag gcaggtgaaa tacagggaga tcgcctgcag
     1981 gcagaaggag gcgacttcgt gctcctgccg gtcccagaag gtatcgagaa tatagaggat
     2041 gctgtccggg cgcatcgcgg cggcggcccg ctgcaaaaga tgcaggacgt cggcttccga
     2101 gaagcacacc aggaattggc tcatccagat ggcatcgaat cccgccggga agggttgcgt
     2161 gtgatcgagc aggtcgatgg ctttggcatt gatgcgagcc tgaagcccca ggcgttcgac
     2221 gttctgcttg gcggcggcta cctgtccggg aagatcgagg atcgtgatcg agacgtccgg
     2281 cgagtgctgc gcgcagcgga tcgcccactt cccggtattg ccacccacgt ccagcagcgc
     2341 tcgcggcgag ccctcgaaaa cgacgggcag ggcctgcgcg aatgccgcat cggaatagta
     2401 gtggtcccac cgcagccagc tggcccgcac ggcttcgggt agagcgggca gcgcctggta
     2461 gatcgtgtcc cactcgccga agactttcct cagcccggcg ggctcgccct gccggatgga
     2521 atcctccagg tagtacatcg cctggaagca gccttgctgc acgaaatcca tgttgatgcg
     2581 ggtcagttcg tccttcagga tgcaggcgcc ggtcgtggtc agcaggaagc ggctgccttg
     2641 cgcacggacc aggccggcgg cgagaccggc ctcgagaagc accagcacgc catagccgga
     2701 cgtatcgact cgggcgacga tctcctcgag cgcgaggccg gcgggagccg cccgcaaagc
     2761 ctccaggatg cccagctcgc gcagcagcct tgccgcctgg aagatgagcg ggccgaaggc
     2821 gatcttctgc gcctcgaatc gcgcctcgag gagcgaaatc ggctcggcgg attcgatttt
     2881 catggcggga ttgtagcccc cgccatgcgc ccgggacctg cctacggcgc tctctctccg
     2941 ccgcgcgcgc gcgccgtgaa cggatagagc tgccggtcac cggcgcggcg gatcgcctcg
     3001 atgctgtaga gccggctgtg gaacgagtcg agcgctttca ggatctgcag gcgcaggaac
     3061 tccgcgcccg acatcgattc cgactcccac ttgcggtgct cgccgcgcgc gaagagctcc
     3121 gcggcgtagt caccgacgat gccgatttcc tcgcggcatt gcgggtcgtc gaccgcggcg
     3181 gcggcatcgc ggcacagttg ctccacgcgc cgcagctcct cgccgccggc cgtccagtcg
     3241 ccgcggtggt gggcgaaaac ctccttcagg gcggcgtggc tgccgcgcgc ctcgtcgatg
     3301 tccgggtagc tcatcggatg gccgattgtc gccaagcggc gcggcgcttt caatcgcgcg
     3361 cgcaagattc gaggattccg ctacgccctg tcgccggcga tgcgacgggc tggcctcagg
     3421 ttgcgggcta ggttgcgggc tggcgggcgg ccttgcgttc cagcaggcgg gcgaaattct
     3481 ccagcgacag gaggtgcaga tagatgcggg cgagaaagcc cttgcgcatc aggttcttca
     3541 gctgcgccgc gttgaggttc tcgaggttct tttcgttgac gcgctgcatg ccggtgagct
     3601 gcatggcgcc gccgcccttc tccttgttga gcgtcgcctg catcgtgaag ggctcgagca
     3661 gcccgtagtc ggcgagcacc gcgcacatct cgcggctgcg ctcgaggtcc gcctcgtact
     3721 cggtgagcag gcgctcgatg ccggtccact tctgcgccgg cttgccttgc gcgtcgaaca
     3781 tcgcttcgcc cgcggcttcg tcgatggccg ccttctccac gcagatcagg cggtcctgct
     3841 gcgcgacctg gttgagcgtg acgcgcgcca tgcagaacgg atagcggcgc gaatacgccg
     3901 ggaggtagac gccggccgcc caagcgccat cggcgaaaaa cagattctcg ccggctgcga
     3961 tgccgaggac cgcgaccgca gcaaaggtct tgccctcgtc gccggtggtg aacacgatcg
     4021 cgtactcgcg cgccgcggcc tggaactccg tgtggctgat cggcaccgca ttggcgcggc
     4081 gcaggaattc gggcaccgcc cccgccgccg gcaggcgcag ccggtgcgtc ttcagcaggg
     4141 ggacgacctc ctggtagccg aaaggcggcg cgatgctggg tggggcgggc ggcatggccc
     4201 gccgataata cggtaaaatc cgcagccttc gacggggtgg tagctcagct gggagagcgt
     4261 cgcgttcgca atgcgaaggt cgagggttcg atccccttcc actccaccat ctttcccgcg
     4321 cagtccctga agtttcttcc tgaagttact atgccgccat ggaagcgcat cgcctggtgg
     4381 cggcggcgct gcagcggatc gtcgataagc tcgatccgca cgagggccgc taccgcatgc
     4441 tgttcgagtc gagcccgctg ccgatgtggg tgttcgacgc cggcacgctg cgctatctcg
     4501 cggtcaacga cgccgcggtg cgcctgtacg gctacagccg cgacgagttc ctcggcatga
     4561 cggtggagga cctgcgcccg cagggcgagg gcgagggcga ggcgttccgc gagttcctgc
     4621 gccgcgagtc gggctccatg cagcgcggcc tgttccacca cctcaagaaa ggcggcgagc
     4681 cgatcgacgt cgagaccgtc gggcacctgg tgcgctggcg cggccgcgag gcgcgcctgg
     4741 tgcagatcaa cgacgtcacc gagcggctgc gcgtgcagac gcggctgcgc gcgctgtcgc
     4801 agcggctgct cgaggtgcag gaggaagagc ggcgcagcct ggcgcgcgac ctgcacgacg
     4861 acatcggcca ggcgctgact gcgctaaaga tccagttgga gtcgctggcg cagtcgcagt
     4921 cgggcgaggg cgagccgcgg cggcgcgtcg atgaatgcgt cgagaccacg cgccacaccc
     4981 tcgagcgcgt gcgccagctg tcgctcaacc tgcgcccgct gcagctcgac gatctgggtc
     5041 tggtggcggc gctgcgctcg cacctcgatc ggcaagcggg gctgggcggg ctcgcgccgc
     5101 acttcgacgc gggcgaggcg ccgccgcagc tcgacccgga gatcgagacc gcctgcttcc
     5161 gcgtcgccca ggaggcgatc accaacgtgc tgcgccatgc gcgcgcgcgc aacctgtggg
     5221 tgcgcctgtt taccaccggg gaccggctcg cgctctcggt gcgcgacgat ggcggcggct
     5281 tcgatccgga cagcgcccgg cgccgcgcgc acgccggcgg cagtctcggc cttgccggca
     5341 tggaagaacg catctccatt gccggcggga cgatcgaatt gcgctcccgg cccggacagg
     5401 gaacggtttt gctggcaaca ttccccatcg agcgagcagg ggggcagggg cgatgatgat
     5461 ccgcgtcgtg ctggccgacg accacgcgct ggtgcgcgcc ggcatccgct cgctcctcgg
     5521 caacatggcc gaagtggcag tggtgggcga ggcctcgagc ggcgaggaag cgatgctgct
     5581 cgccgaaagc gagcggcccg acgtgatcct catggacatc gccatgaagg gcatcagcgg
     5641 gctggaagca gccgcgcacc tgcgcgaaag ccatcccgcc gttcgcgtgg tgatcctttc
     5701 gatgcattcc ggagaggagt acgtcctgca ggcgctgcgc gcgggcgctg tgggctacct
     5761 gctcaaggac gccgccaccg gcgagctcga gctcgccctg cgcagcgtga tgcgcggcga
     5821 gagctggctc agtccggccg tgtcgcgcca ggtagtcgaa ggctacgtgc agcgcgttgg
     5881 cggcgacgcc gcgcccgagg tgctcaccgc tcgccagcgc gaggtgctca agctcgtcgc
     5941 cggcggcaag agcacgaagg aaatcgcctt tctgctcaat ctctcggtga aaaccgtgga
     6001 gacgcaccgc gcgcagatca tggagcgcct gggcatccgc gacgttgcgg gcctggtgcg
     6061 ctacgcgctg cgcaccgggc ttattcctcc cgagggttga ccgtttttca cggcaattga
     6121 caaggtttca cagggaatca gggatttcgt aaccccctcc tcggggaccc cccgatacct
     6181 ggggtgttgg gtggcccgta ctctgggacg catggcccgt acccgccgca tcgcgatcgc
     6241 cgacgacagc cctgcttttg tttcggcggt atcgaactac gtcgagagcc tgccgggcta
     6301 cgagctcgcc ggcaccgcgc gcacgtcggt gcaggcgctc gcactggtga agtcccaggt
     6361 gctcgacgtg ctgcttctcg acctcggcct gccgcccgtg cgcggtctcg aattcgtgcg
     6421 gcggctgaag gcgctgccgg gcggtccggc ggtcgtcgcg atgaccctgt tccacactcc
     6481 tgaagccgct gccgaagccc ggcgcgccgg cgccgacgcg ctggtcggca aggaggcgtt
     6541 cgtagccggg ctgaaccagg cgctggcgaa gctcttcccc accgccagct agcatgagcg
     6601 ccggcttgcc gaggcagcct ctcatcgagg agcgcttgcg cgccaccttc atgatgtacg
     6661 ccgcgttgtc ggcagccaac gaggcgatcc tgcgcgccaa gtcgccgcag gaagtgttcc
     6721 acagcgtctg tgacatcgcg gtgcagaccg gcggcttcct gctcgccacg gtgttcaggc
     6781 tggacgcgca agccggcttg ctggagcgcg tcaccgccag cgggccg
//
LOCUS       DASYJA010000179         3463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1844573, whole genome shotgun sequence.
ACCESSION   DASYJA010000179 DASYJA010000000
VERSION     DASYJA010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1844573"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1038
                     /locus_tag="VGO02_03865"
     CDS             85..1038
                     /locus_tag="VGO02_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7476856.1"
                     /translation="MRLVFLTVIFLAEIANAQTYPTKPIRLLVPFAPGGSSSIVARLA
                     AEEMSKGLGQSIVIENKGGGGGNLAMQEAAHAEPDGYTLIIGHIGSLAVNPYMYEKLP
                     YDVDRDFTAVSLLAIVPAIFVVFHEVPAKDLRELVALAKKKPGELFYGSAGNGSAGHL
                     AMEYLKQAAGIDLKHVPYKGTGPNVTDLIAGRTQVASAGTPPLMPHVKSGKLRVVAVG
                     GAKRLASMPEVPTVAEQGYPGFQTSQWYGLNAPAKTPEAVIRRLAAEAAKAAKSPLML
                     ERLAVDDAEAVGSTPEEYAAFIKREQERWSKVVRTANIKAD"
     gene            1053..2030
                     /locus_tag="VGO02_03870"
     CDS             1053..2030
                     /locus_tag="VGO02_03870"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7476857.1"
                     /translation="MKAPRSFTRLFAAVALACCASAAPAQQPYPAKPIRVLIGFSAGS
                     EIDTIGRLVVQKMGEGLGQQLIVENRLGAGGTLAAAQMASAAPDGYVLLINSVSHAGV
                     QALYPNLPFDTVRDFAGISQLTSAPNVLVVAPSQGIKTAGELIALAKQKPGQVNFGSA
                     GVGSGTHMTLEQFRLATGIQVTHVPYKGVPEVLTDTATGRVNTSFAPIGNTLAMIKDG
                     RLVPLAVSTAVRSPALPDVPTLSETAVPGLDWDQWYGMFAPAKTPRPIVNQLSREVAR
                     VLALPDIKERIANRGSTPKPSSPEEFDAFVRAEVAKVGKVIRDGNIRID"
     gene            2030..2380
                     /locus_tag="VGO02_03875"
     CDS             2030..2380
                     /locus_tag="VGO02_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV7476858.1"
                     /translation="MKKKLAFVEKMARLPWKEFPGHFGGALSKMLVGTERLDFRISRY
                     APNAYVQEHSHKVQEQIYYVLEGEGVLTVGKEKHLMQPHDYVYLPPGVPHSFTNTGLS
                     GLVFLVVTTPADDE"
     gene            2389..>3463
                     /locus_tag="VGO02_03880"
     CDS             2389..>3463
                     /locus_tag="VGO02_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEV7476859.1"
                     /translation="MKGGELIAEYLIKQKVPYLFGICGHGNVGILDPLHAVRDRLKLI
                     SPRHEQCAGHMADGYFRVRHQPVATLTSTGPGSANLVMSLATALSDSSAFLAITSNVP
                     TSQMNRAPFQEIYKHNQADFPSVIRPVVKRAFQPTRVDMLPLALRQAFDTMVTGRPGP
                     VNIDIPYNVYQEEGDVEVPPASHVFGQHRPGASEEDLLKTVNLLADAMRPVIFIGHGA
                     TLSEAGPELTRLQQTYGIPVITSPNGMGCVPADDPLTLGFIGRNGAYPANQAGRHTDL
                     VLTIGTRFDDRSSSSWQPGYSWNFPKTKLIHVDLDPQELGRNYPPTLGIIADAKVFTA
                     QLLAALGLREQRGAAPLKRQYSEW"
BASE COUNT          617 a         1193 c         1116 g          537 t
ORIGIN      
        1 ctcgcacatg ctgatgatgg accgcaacaa cctcgaggtc gctgcgctcg tccaggcgtg
       61 gctcgagaag cagggcttgt acagatgaga ttagtttttt taacagtaat ttttttagcg
      121 gaaattgcga acgcgcagac ctatcccacg aaacccatcc gcctgctcgt gcctttcgcc
      181 ccgggcggca gctcgtcgat cgtggcgcgc ctggcggcgg aggagatgtc gaagggcctc
      241 ggccagtcga tcgtgatcga gaacaagggc ggcggcggcg gcaacctcgc catgcaggag
      301 gcggcgcacg ccgagcccga cggctacacg ctgatcatcg gccacatcgg ctcgctcgcc
      361 gtgaacccgt acatgtacga gaagctgccc tacgacgtcg accgcgactt caccgcggtg
      421 tcgctgctcg ccatcgtgcc cgcaattttc gtcgtcttcc acgaggtgcc ggcgaaggat
      481 ttgcgagagc tggtcgcgct ggcgaagaag aaaccgggcg agctgttcta cggcagcgcc
      541 ggcaacggct cggccgggca cctggcgatg gagtacctca agcaggcggc cggcatcgac
      601 ctgaagcacg tgccctacaa gggcaccggg ccgaacgtca ccgatctgat cgccgggcgc
      661 acgcaggttg cctcggcggg gacgccgccg ctcatgccgc acgtgaagag cggcaagctg
      721 cgagtggtcg ccgtcggcgg cgcgaagcgg ctcgcctcga tgcctgaagt gccgacggtc
      781 gccgagcagg gttacccggg gttccagacc tcgcagtggt atggcctgaa cgcgccggcg
      841 aagacgcccg aggcggtgat caggcggctt gccgccgaag cggcgaaggc ggcgaagagc
      901 ccgctgatgc tcgagcgcct ggcggtcgac gacgccgagg cggtcggctc gacgccggag
      961 gaatacgcgg cgttcatcaa gcgcgagcag gagcgctgga gcaaggtagt gcgcactgca
     1021 aacatcaagg ctgactgaca ggaggaacgt ccatgaaggc accacgcagc ttcaccaggc
     1081 tcttcgccgc ggttgcgctc gcttgctgcg ccagcgctgc gccggcgcag cagccttatc
     1141 ccgccaagcc gatccgcgtg ctcatcggct tttccgccgg cagcgagatc gacaccatcg
     1201 gccgcctggt cgtgcagaag atgggcgagg ggctgggcca gcagctgatc gtggaaaacc
     1261 gcctgggggc cggcggcacg cttgccgccg cgcagatggc gagcgccgcg cccgacggct
     1321 acgtgctgct catcaattcg gtgtcgcacg ccggggtgca ggcgctctac ccgaacctgc
     1381 ccttcgacac ggtgcgcgac ttcgccggga tctcgcagct gaccagcgct cccaacgtgc
     1441 tggtggtcgc tccctcgcaa ggaataaaga ccgcgggaga gctgatcgcg ctcgcgaagc
     1501 agaagcccgg acaggtgaac ttcggctcgg ccggggtcgg cagcggcacg cacatgacgc
     1561 tcgagcagtt caggctcgcc accggcatcc aggtcaccca cgttccctat aagggcgtgc
     1621 ccgaagtgct gaccgacacg gccaccggcc gcgtgaacac gtccttcgcc ccgatcggca
     1681 acacgctcgc catgatcaag gacggccgcc tcgtgccgct cgcggtgagc accgccgtgc
     1741 gctcgccggc gctgcccgac gtgccgacgc tgtcggaaac cgcggttccc ggcctcgact
     1801 gggaccagtg gtacggcatg ttcgccccgg cgaagacgcc gcgtccgatc gtgaaccagc
     1861 tgtccaggga agttgcccgc gtgctcgcgc ttcccgacat caaggagcgc atcgccaacc
     1921 ggggatcgac gccgaagccc tcgtcgccgg aggaattcga cgccttcgtc cgcgccgagg
     1981 tggcgaaggt cggcaaggtg atccgcgacg gcaacatccg catcgactga tgaaaaagaa
     2041 gctggccttc gtcgagaaga tggcgcgcct gccgtggaaa gagtttcccg gtcatttcgg
     2101 gggtgcgctt tccaaaatgc tcgtcggcac cgagcggctc gacttccgca tctcgcgcta
     2161 cgcgcccaat gcgtacgtgc aggagcattc gcacaaagtc caggagcaga tctactacgt
     2221 gctggagggc gagggcgtcc tcaccgtcgg caaggagaag cacctgatgc agccgcacga
     2281 ctacgtctac ctgccgccgg gcgtgcccca cagcttcacc aacaccggcc tttcggggct
     2341 ggtgttcctg gtcgtgacta ctcccgcgga cgacgaataa tggcaaagat gaaaggcggc
     2401 gagctgatcg ccgaatacct gatcaagcag aaggtgccgt acctcttcgg catctgcggg
     2461 cacggcaacg tcggcatcct ggacccgctg cacgcggtgc gcgacaggct gaagctgatc
     2521 tcgccgcgcc acgagcagtg cgccggccac atggccgacg gctacttccg cgtcaggcac
     2581 cagccggtgg cgacgctgac ctcgaccggc ccgggctcgg cgaatctcgt catgtcgctc
     2641 gccaccgcgc tgtcggattc ctcggcgttc ctggcgatca cctccaacgt gccgacctcg
     2701 cagatgaacc gcgcgccgtt ccaggagatc tacaagcaca accaggccga cttcccctcg
     2761 gtgatccgcc cggtggtgaa gcgcgcgttc cagcccacgc gcgtcgacat gctgccgctc
     2821 gcgctgcgcc aggccttcga caccatggtc accggtcgcc ccgggccggt gaacatcgac
     2881 atcccctaca acgtctacca ggaggagggt gacgtcgaag tgccgcccgc ctcgcacgta
     2941 ttcggccagc accggccggg agcttctgaa gaagatttat tgaaaacagt aaatctgctg
     3001 gctgacgcca tgcgcccggt aatcttcatc ggccacggcg cgacgctctc cgaggccggt
     3061 cccgagctca cccggctgca gcagacctac ggcattcctg tcatcacttc gcccaacggc
     3121 atggggtgcg tgcccgcgga cgatccgctg acgctcggct tcatcggcag gaacggcgcc
     3181 taccccgcga accaggcggg gcgccacacc gacctggtcc tcaccatcgg cacgcgcttc
     3241 gacgaccgct cgtcctcgag ctggcagccg ggctactcgt ggaatttccc caagaccaag
     3301 ctgattcacg tcgacctcga cccgcaggag ctcggccgca actatccgcc gacgctcggc
     3361 atcatcgcgg atgccaaggt cttcaccgcc cagctcctcg ccgcgctcgg cctgcgcgag
     3421 cagcgcggcg ccgcgccgct caagcgccag tactccgagt ggg
//
LOCUS       DASYJA010000180         3451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_119164, whole genome shotgun sequence.
ACCESSION   DASYJA010000180 DASYJA010000000
VERSION     DASYJA010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119164"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1376)
                     /locus_tag="VGO02_03885"
     CDS             complement(81..1376)
                     /locus_tag="VGO02_03885"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HEV7476860.1"
                     /translation="MAGPKYPGFDTLALHAGQSPDPTTGSRAVPVYQTTSYVFRDTEH
                     AASLFNMERAGHVYSRISNPTVAVLEERIAALEGGVGAIATSSGQAALHLAVATLMGA
                     GGHIVASGSLYGGSHNLLGYTLKRFGIETTFVNGRNLDDWKKAVRENTRLLFGETLGN
                     PGIDVLNIPAIAGIAHQAKLPLLVDATFTTPWLMKPFELGADLLYHSATKFLGGHGVA
                     IGGVLVDSGRFDWEASGKFETLTAPYAGFHNMDFEEESGTRAFLLRARREGLRDFGAC
                     MAPMTAFQLLQGVETLHLRMPRHVENTRKVVQFLSSHPAVEKVLYPELPSHPDYELCR
                     QLLPNGAGAVFSFDIKGGRAAGKAFIESLRVFSHLANVGDAKSLVIHPATTTHYRMSD
                     EDLKKSGIGPGTVRLSVGLEDAQDLIEDLDRALKAAARV"
     gene            complement(1377..1931)
                     /locus_tag="VGO02_03890"
     CDS             complement(1377..1931)
                     /locus_tag="VGO02_03890"
                     /inference="COORDINATES: protein motif:HMM:NF042855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6806 family protein"
                     /protein_id="HEV7476861.1"
                     /translation="MRLEVHVHADIPILDGVSKRQLEQAFAPLLEYLDADGLADVKSL
                     EPDEPGLKYDEKDMILTLCWTGEIGRSFHGALEAALDNLGPYCYEAVEVDVTQYHENG
                     EQESKLLFVGPTAQAIHEAQRRCMIEDLSAILGRQFGKTEVAEVATLVNDLFDRDWKE
                     KASQNKKPERAFETYTRPSRKNLH"
     gene            1991..3133
                     /locus_tag="VGO02_03895"
     CDS             1991..3133
                     /locus_tag="VGO02_03895"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEV7476862.1"
                     /translation="MKPDYTGGGLVNLMASIVAARGGSPRHSLLASLPLRDISGATNI
                     LLLIIDGLGDGYLQRRGAGGELARRRRAAITSVFPPTTAAAITTSYTGCTPLEHGLTG
                     WYTYFGAAGCVSAVLPFKSRGDQLPLTDRGVTAGQIFVEAPLFQRIPVRSIVVTYRPL
                     IDSDYNVRHCEGAERHGYETLDGFVSEIEKAVKSGPQRKFIYAYWPQYDTTSHRYGSE
                     SARAAEELEKIDAAFGRLLARLKGSDTLVIATADHGFVDSAPEESLELPSSLASQLRF
                     PLCGERRVAYCYVHSQKNFLQEAKDHFGDRAEVRPSRELLDEGWFGPGAPHPSFAERI
                     GDVALVMRGRYTIKDWTPGEARYLHIGNHGGTSADEMMIPLITEAA"
     gene            3130..>3451
                     /locus_tag="VGO02_03900"
     CDS             3130..>3451
                     /locus_tag="VGO02_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7476863.1"
                     /translation="MKAKVNGIEINYEVSGQEGAPWLVLSHSLACTVRMWDPTIDAFK
                     ASYRILNYDMRGHGQTSAPAVPYSLDMLANDVLELMKFLKIQRASYCGLSIGGMIGQT
                     IAIRG"
BASE COUNT          596 a         1127 c         1118 g          610 t
ORIGIN      
        1 aaccatggtc ggcttgcttg cgtcgagctc gtgggcggcc gtatacgcga aagctttttt
       61 attttctaga agaaactcca tcaaaccctg gcggcggcct tcaaggcccg gtccaggtcc
      121 tcgatcaggt cctgcgcgtc ctccaggccg accgacaggc gcacggtgcc gggcccgatg
      181 ccggatttct tcaagtcctc atcgctcatc cggtagtgcg tggtggtagc cgggtggatg
      241 accagcgact tggcgtcgcc gacgttggcg aggtgcgaga acacgcgcag cgactcgatg
      301 aacgccttgc ccgcggcgcg gccgcccttg atgtcgaacg agaacaccgc gccggcgccg
      361 ttgggcagga gctgcctgca aagctcgtag tcgggatggc tcggcagctc cggatagaga
      421 actttttcaa cggcaggatg cgacgaaaga aactgcacca ccttgcgggt attctcgacg
      481 tgcctcggca tcctgagatg cagcgtctcg acgccctgca ggagctggaa ggcggtcatc
      541 ggcgccatgc aagcgccgaa gtcgcgcagg ccctcgcgcc gcgcgcgcag caggaaggcg
      601 cgcgtgcccg actcctcctc gaaatccatg ttgtggaagc cggcgtaggg cgcggtcagc
      661 gtctcgaact tgcccgaggc ctcccagtcg aagcggcccg agtcgaccag cacgccgccg
      721 atggcgacgc cgtggccgcc gaggaacttg gtcgccgagt ggtacagcag gtcggcgccg
      781 agctcgaacg gcttcatcag ccagggcgtg gtgaaggtgg cgtcgaccag cagcggcagc
      841 ttcgcctgat gggcaatccc ggcgattgcc gggatgttca ggacatcgat gccgggattg
      901 ccgagggtct cgccgaacag cagcctggtg ttttccctga cggctttctt ccagtcgtcg
      961 agattgcgtc cgttgacaaa ggtcgtctcg atgccgaagc gcttgagggt gtagccgagc
     1021 aggttgtgcg agccgccgta aagcgagccc gacgccacga tgtggccgcc cgcgcccatc
     1081 agcgtggcga ccgcgaggtg cagcgcggcc tggccggaag acgtcgcgat cgcgccgacg
     1141 ccgccttcca gcgccgcgat gcgctcctcc agaacggcga ccgtggggtt cgagatgcgc
     1201 gagtagacgt gccccgcgcg ctccatgttg aaaagcgacg cggcgtgctc ggtgtcgcgg
     1261 aagacgtacg aggtagtctg gtagaccggg accgcgcggc tgccggtcgt cgggtcaggt
     1321 gactgcccgg cgtgcaacgc cagcgtatcg aaacctgggt acttcggccc cgccacttag
     1381 tgcaggtttt ttctcgacgg gcgcgtgtac gtctcgaacg cgcgctcggg cttcttgttc
     1441 tgcgaggcct tttccttcca gtcgcggtcg aagaggtcgt tcaccagcgt ggccacctcc
     1501 gccacttcgg tcttgccgaa ctggcggccg aggatcgcgc tgagatcctc gatcatgcag
     1561 cggcgctgcg cctcgtggat cgcctgcgcg gtcgggccga cgaacaggag cttcgactcc
     1621 tgctcgccgt tttcgtggta ctgcgtgacg tcgacctcga ccgcctcgta gcagtacggc
     1681 ccgaggttgt cgagcgcggc ttcgagcgcg ccgtggaagc tgcggccgat ctcgccggtc
     1741 cagcacagcg tgaggatcat gtccttctcg tcgtacttca ggccgggctc gtcgggctcg
     1801 aggctcttca cgtcggcgag gccatcggcg tcgaggtact cgagcagcgg cgcgaacgcc
     1861 tgctccagct gccgcttgga gacgccgtcg agaatcggga tgtcggcatg aacatggact
     1921 tcgagccgca taaaaaaacc tctggagggg atggccaatt ctaccgctaa aatcatccgc
     1981 gaaatccctt atgaaaccag actatacggg cggcgggctg gtcaatctga tggcctccat
     2041 cgtggccgcc cgtggcggct cgccgcgcca ttcgctcctg gcatccttgc cgctgcggga
     2101 tatttccggc gcgacaaaca tcctgctcct gatcatcgac ggcctcggcg acggctacct
     2161 gcagcgccgc ggcgcgggcg gcgagctggc gcggcgccgg cgcgccgcga tcacctccgt
     2221 ctttccgccg acgaccgccg cggcgatcac cacctcctac accggctgca cgccgctcga
     2281 gcacgggctc accggctggt acacgtactt cggcgccgcc ggctgcgtct cggccgtgct
     2341 gccgttcaag agccgcggcg atcagctgcc tttgaccgat cggggagtca cggccggcca
     2401 gatctttgtg gaagcgccct tgtttcaaag aattcctgtc cgctcgatcg tcgtcaccta
     2461 ccggccgctc atcgactccg actacaacgt gcgccactgc gagggcgccg agcggcatgg
     2521 ctacgaaacg ctggacggtt ttgtttctga aattgaaaaa gcagtgaaat ccgggccgca
     2581 gcgtaaattc atctacgcct actggccgca gtacgacacc acctcgcacc gttacggctc
     2641 ggagagcgcg cgggccgccg aagagctcga gaagatcgac gccgccttcg gccggctgct
     2701 ggcgcgcctc aagggcagcg acacgctggt gatcgccacc gcggatcatg gcttcgtcga
     2761 cagcgcgccg gaagaatcgc tcgagctgcc ctcttccctc gcttcgcagc tcagatttcc
     2821 cctgtgcggg gagcggcggg ttgcctattg ctacgtgcac tcacagaaga atttccttca
     2881 agaagcaaaa gaccattttg gcgatcgtgc cgaggtacgt ccgagccgcg agctgctcga
     2941 cgaaggctgg ttcggccccg gcgcgccgca cccgagcttc gccgagcgca tcggcgacgt
     3001 cgcgctggtc atgcgcggcc gctacacgat caaggactgg acgcccggcg aggcgcgcta
     3061 cctgcacatc ggcaaccacg gcggcaccag cgccgatgaa atgatgatcc cactgataac
     3121 ggaggcggca tgaaggcaaa ggtcaacggc atcgagatca actacgaagt gagcggccag
     3181 gagggcgcgc cgtggctggt gctcagccat tcgctcgcct gcaccgtgcg catgtgggac
     3241 ccgaccatcg acgccttcaa ggcgagctac cgcatcctca actacgacat gcgcggccac
     3301 ggccagacct cggcgccggc ggtcccgtat agcctggaca tgttggccaa tgatgttctt
     3361 gaactcatga aatttctgaa aatacagcgg gcgtcgtact gcggcctgtc catcggagga
     3421 atgatcggac aaaccatcgc catccgcggc g
//
LOCUS       DASYJA010000181         2555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_937543, whole genome shotgun sequence.
ACCESSION   DASYJA010000181 DASYJA010000000
VERSION     DASYJA010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937543"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="VGO02_03905"
     CDS             <1..1204
                     /locus_tag="VGO02_03905"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEV7476864.1"
                     /translation="TQAGRALLARQPFSPDQLDEVILGCAAPSVDEVNIGRVVVLCMG
                     CGQKVPGWTVMRNCASGMQALDSGINNILAGRSNLVLAGGVDALSRAPLLYNDKMVLW
                     FSALAAARSTSQKVSLFGRLPVKAMLAPVIGIMKGLTDPMVGLLMGQTAENLAHRFGI
                     TREQQDAFSVRSHQRVISSQKNGYLAPGGGEVEALFDTKGNPYLLDDGVRADASVENL
                     AKLKPFFDRKYGTVTPGNSSQITDGAAWMILASEEGLKKFGLTPIGRIMDSEWAGLDP
                     AQMGLGPVHAATPILKRHGLGLDDLDYWEINEAFAAQVLGCLAAWRDEKYCRDELGLD
                     RALGSLNEEKLNVDGGAIALGHPVGASGARIVLHLLRTLKRNNAKRGIASICIGGGLG
                     GAMLVEAL"
     gene            1204..>2555
                     /locus_tag="VGO02_03910"
     CDS             1204..>2555
                     /locus_tag="VGO02_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEV7476865.1"
                     /translation="MDRAAALTKHFHWETDRDGLAWLTFDKQGESTNTFSREALEQLS
                     SCLDEIGKASPKGLVIRSAKENFIAGADVEEFTRFKSPEEALAFVRLGWDVLQKLRDL
                     PFPTTAMVNGFCMGGGVELALACRYRVALDDPKTRFALPEVMLGIMPAWHGVQWLPKL
                     VGPAAAFDMLLTGKAVDARRAKRIGLVDQAVPLRILENTARMVTLESPKRKKLPVGSR
                     LMLSFRSFVASQARKQVAKRVKREHYPAPYAILELWRKYDGDPFAADQDPAASVRTLF
                     AHPTTANLIRIFFLQERLKAQGKGSAHFPGGEARHVHVIGAGVMGGDIAAVCAMRGLT
                     VTLQDTSPERLAPAVKRAAQLFERRLREPRRVRDAFDRLIPDVSGLGAGQADVIIEAI
                     FENLQAKQDLFSHLEKIARPGAILASNTSSLKLSDIGTVLKDPSRLVGIHFFNPVALL
                     "
BASE COUNT          449 a          841 c          819 g          446 t
ORIGIN      
        1 cacgcaggcc gggcgcgcgc tgctcgcccg ccagcccttt tcgccggatc agctggatga
       61 agtcatattg ggctgcgccg cgccttcggt cgacgaggtc aacatcggcc gcgtcgtcgt
      121 gctgtgcatg ggctgcggcc agaaggtccc cggctggacc gtgatgcgca actgcgcctc
      181 cggcatgcag gcgctcgatt cgggcatcaa caatattctg gccgggcgct ccaacctggt
      241 gctggctggc ggagtggatg ccctgtcccg ggccccgctg ctctacaacg acaagatggt
      301 gctgtggttt tccgccctgg ctgctgctcg ctctacatca caaaaagttt cgttattcgg
      361 aaggttaccg gtgaaggcga tgctcgcccc ggtcatcggc atcatgaaag gcctcaccga
      421 cccgatggtc ggattgctca tgggccagac cgcggagaac ctggcgcatc gcttcggcat
      481 cacgcgcgag caacaggacg cattttctgt ccgctcgcat caacgagtta tttcatctca
      541 aaaaaatgga tatctagcgc cgggcggtgg cgaagtcgaa gctttgttcg acaccaaggg
      601 caatccttac ctgctcgacg acggcgtgcg cgccgacgcc tcggtcgaga atctcgccaa
      661 gctcaagccc ttcttcgacc gcaagtacgg cacggtgacg ccgggcaaca gctcgcagat
      721 caccgacggc gcggcgtgga tgatcctggc gtccgaagag ggtttgaaaa agtttggttt
      781 aacccccatt ggcagaatca tggattccga atgggccggc ctcgatccgg cgcagatggg
      841 cctcggcccg gtgcacgccg cgacgccgat cctcaagcgc cacggcctcg ggctcgacga
      901 tctcgactac tgggagatca acgaggcgtt cgccgcccag gtgctcggct gcctcgcggc
      961 gtggcgggac gagaagtact gccgcgacga gctcggactc gatcgtgctt taggttcttt
     1021 gaacgaagaa aaactcaacg tggacggcgg cgccattgcg ctcggccacc cggtcggcgc
     1081 ttcaggcgct cgcattgttt tgcatttatt gagaacattg aaaagaaaca atgcaaaacg
     1141 gggcatcgcc agcatctgca tcggcggcgg cctgggcggc gcaatgctgg tggaggcgct
     1201 ctgatggacc gcgccgccgc cctgaccaag cacttccatt gggaaaccga ccgcgacggc
     1261 ctcgcctggc tgactttcga caagcagggc gagtcgacca acacgttctc gcgcgaggcg
     1321 ctggagcagc tttcttcctg cctggatgaa ataggcaaag cttcacccaa gggcctggtc
     1381 atccgctcgg cgaaggagaa cttcatcgcc ggcgccgacg tcgaggagtt cacgcgcttc
     1441 aagtcgccgg aggaggcgct cgccttcgtg cgcctgggct gggacgtgct gcagaagctg
     1501 cgcgacctgc ccttccccac caccgcgatg gtcaacggct tctgcatggg gggcggcgtg
     1561 gagctcgccc tcgcctgccg ctaccgcgtc gcgctcgacg atccgaagac gcgcttcgcc
     1621 ctgcccgaag tgatgctcgg catcatgccg gcgtggcacg gcgtgcaatg gctgccgaag
     1681 ctggtcggcc cggcggcggc gttcgacatg ctgctcaccg gcaaggcggt cgacgcgcgc
     1741 cgcgcgaagc gcatcggcct ggtcgaccag gcagtgccgc tgcgcatcct ggaaaacacc
     1801 gcgcggatgg tgactctcga gtctccgaag agaaagaaat tacccgtggg aagccgattg
     1861 atgctttcct tcagatcatt cgtggcctcg caggcgagga agcaggtggc gaagcgcgtg
     1921 aagcgcgagc actacccggc gccctacgcg atcctcgagc tgtggcgcaa gtacgacggc
     1981 gatccgttcg ccgccgacca ggacccggcg gcctcggtca ggacgctgtt cgcccatccg
     2041 accaccgcca acctgatccg catcttcttc ctgcaggagc gcctcaaggc gcagggcaag
     2101 ggcagcgctc attttccagg cggcgaggcg cgccacgtgc acgtcatcgg cgccggcgtg
     2161 atgggcggcg acatcgccgc ggtgtgcgcg atgcgcggcc tgacggtgac gctgcaggac
     2221 acctcgcccg agcgcctggc gcccgcggtg aagcgcgccg cgcagctctt cgagcgccgg
     2281 ctgcgcgagc cgcgccgcgt gcgcgacgcc ttcgaccgcc tgatcccgga tgtcagcggc
     2341 ctgggcgccg ggcaggccga cgtcatcatc gaggcgatct tcgagaacct ccaggcgaag
     2401 caggaccttt tttcccacct ggaaaaaatt gccaggccgg gagcgatcct cgccagcaac
     2461 acctccagcc tcaagctctc cgacatcggc acggtgctga aggatccctc gcggctggtc
     2521 ggcattcact tcttcaatcc ggttgcgctt ctgca
//
LOCUS       DASYJA010000182         4006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_392512, whole genome shotgun sequence.
ACCESSION   DASYJA010000182 DASYJA010000000
VERSION     DASYJA010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392512"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VGO02_03915"
     CDS             <1..718
                     /locus_tag="VGO02_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEV7476866.1"
                     /translation="EGMRPVVAIYSTFLQRGYDQLIHDVAIQNLPVLFALDRGGLVGG
                     DGATHNGAFDYAYLRAVPNLTVMAPSDAGECRDMLYTGCLLGTPAAVRYPRGAAPGDA
                     TREMKALPIGKGEIRRQGRKIAILAFGAMLQPALEAAEKLDATVANMRFVKPLDAELI
                     EKLASTHDLLVTVEEHQAMAGAGSAVCEVLTEISYKKQVLILGLPDRFIDHGDPGRLL
                     ASVGLDAAGIQKSIQNVIAE"
     gene            744..1550
                     /gene="folE2"
                     /locus_tag="VGO02_03920"
     CDS             744..1550
                     /gene="folE2"
                     /locus_tag="VGO02_03920"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HEV7476867.1"
                     /translation="MNARDLQNSIPDVQATPDSRKLAIDKVGIKAIRHPIQIKERSGG
                     VQHTIATFNMYVSLPHQFKGTHMSRFVEILNAHEREITVETFQVMLQEMVEKLEAEEG
                     RIEMTFPYFVDKAAPVSGVRSLMDYEVTFVAQILKGEKCFAMKVLVPVTSLCPCSKQI
                     SDYGAHNQRSHVTVTAHTGDFVWIEEIIDLIEKQASSELYGLLKRPDEKYVTERAYDN
                     PKFVEDMVRDVAALLNLDERITRYVVESENFESIHNHSAYALIERDKRKQ"
     gene            1634..2269
                     /locus_tag="VGO02_03925"
     CDS             1634..2269
                     /locus_tag="VGO02_03925"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEV7476868.1"
                     /translation="MVLEVEAMYAETRSETGLAAMSPAVRAAMSKVERHRLVQDAHRG
                     AAYRNHPLPIGSGQTISQPYIVALSTDLLGLTGNSVVLDVGTGSGYQAAVLAEIASRV
                     FSIEIIPSLGEEARRRLEGLGYGNIEVKIGDGYQGWPEKAPFDAIVVTAAAPRVPPAL
                     VAQLKTGGRMVIPIGGESEIQYLKLITRRADGGYDEKRVLPVRFVPLVPGK"
     gene            complement(2270..2890)
                     /locus_tag="VGO02_03930"
     CDS             complement(2270..2890)
                     /locus_tag="VGO02_03930"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEV7476869.1"
                     /translation="MLFVLLACGAARAADVALIGLIGSGAAVFAVDGGEPRTIKLGQT
                     RAGVTLVSVEGERATVEFEGKRRVLALGQHYRGAGTAVSSGQSVTLAADPRGHFVSEG
                     SINGHPMRFLVDTGATTIALPAADAVRLGINYRSGASVRTRTAAGDASAFRVTLASVR
                     LGDIELTNVEGIVIEDGLDIALLGMSFLNRLDMRQEGRTMTLIKRF"
     gene            2907..3620
                     /locus_tag="VGO02_03935"
     CDS             2907..3620
                     /locus_tag="VGO02_03935"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEV7476870.1"
                     /translation="MKPVGIFRYARTEGPGYFATYLERRSIEWQLIALDEGAAVPRDP
                     RRFSGLVFMGGPMSVNDELPWIVPVLELIREAVRRDVAVLGHCLGGQLMSKAFGGAVR
                     ANPVKEIGWGDVRVADNALAREWLGELQSFESFHWHGETFSIPPGATRLLENAHCVNQ
                     GFALGKHLGLQCHVEMTEELVRSWLKSGADEIKDSSASPAVQQPAEIERDLPARLGAL
                     HEVASRRYDRWTKNLSPGN"
     gene            complement(3604..>4006)
                     /locus_tag="VGO02_03940"
     CDS             complement(3604..>4006)
                     /locus_tag="VGO02_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEV7476871.1"
                     /translation="RYDFKGSDARIELKELELSVYADDEFKLGQVMDVLRARLAKRSV
                     DARSLEEGALEKISGDKLKRPMKIKVGITQEKAKSIVKLLKEAKLKVAGSIQGDAVRV
                     SGAKKDDLQAAIQLVRSNVTDLPLQFINFRD"
BASE COUNT          713 a         1329 c         1298 g          666 t
ORIGIN      
        1 cgagggcatg cggccggtgg tggcgatcta ctcgaccttc ctgcagcgcg gctacgacca
       61 gctgatccac gacgtggcga tccagaacct gccggtgctg ttcgcgctcg accgcggcgg
      121 cctggtcggc ggcgacggcg cgacccacaa cggcgcgttc gactatgcgt acctgcgcgc
      181 cgtgcccaac ctgacggtga tggcgccgtc cgacgccggc gagtgccgtg acatgctcta
      241 taccggctgc ctgctcggca ctccggcggc cgtccgctac ccgcgcggcg ccgcgccggg
      301 cgacgcgacc agggagatga aggccctgcc gatcggtaaa ggcgagatcc gccgccaggg
      361 gcgcaagatc gccatcctcg ccttcggcgc catgctccag cccgccctgg aagccgccga
      421 aaagctcgat gccacggtgg ccaacatgcg cttcgtgaag cccctggatg cggaactgat
      481 agaaaagtta gcgtccactc acgatcttct ggtgaccgtg gaggagcacc aggccatggc
      541 cggagcgggg tccgcggtgt gtgaggttct tactgagatt agttataaga aacaagttct
      601 tattcttgga ttgccggatc gcttcatcga ccacggcgat cccggccggc tgcttgcttc
      661 ggtgggattg gatgccgccg gcatccaaaa atcgattcag aacgtaatag ccgagtaatt
      721 tactcggatg ttaaaatgac cccatgaacg cacgcgacct gcaaaacagc atccccgacg
      781 tccaggccac cccggactcg cgcaagctcg ccatcgacaa agtcggcatc aaggcgatcc
      841 gccacccgat ccagatcaag gagcgctccg gcggcgtgca gcacaccatc gccaccttca
      901 acatgtacgt cagcctgccg caccagttca agggcacgca catgtcgcgc ttcgtcgaga
      961 tcctgaacgc acacgagcgc gagatcacgg tcgagacctt ccaggtgatg ctgcaggaga
     1021 tggtcgagaa gctcgaggcc gaggaaggcc gcatcgagat gaccttcccg tacttcgtcg
     1081 acaaggcggc gcccgtctcg ggcgtgcgca gcctgatgga ctacgaggtg accttcgtcg
     1141 cgcagatcct caagggcgag aagtgcttcg ccatgaaagt gctggtcccg gtgaccagcc
     1201 tgtgtccctg ctcgaagcag atctcggact acggcgcgca caaccagcgc tcgcacgtca
     1261 ccgtgaccgc gcacaccggc gacttcgtct ggatcgagga gatcatcgat ctgatcgaga
     1321 agcaggcttc cagcgagctc tacggcctgc tcaagcgccc cgacgagaag tacgtcaccg
     1381 agcgcgccta cgacaacccg aagttcgtcg aggacatggt gcgcgacgtc gccgcgctgc
     1441 tcaatctcga cgagcgcatc acgcgctacg tggtcgagtc ggagaacttc gagtcgatcc
     1501 acaaccacag cgcctacgcc ctcatcgaga gggataaacg aaagcagtag cggcgctgct
     1561 gtcaggcctg ctcctgttgg aagactccca cgcccaggag agagcagcct cattttccga
     1621 gcttcgctcg cgcatggtcc tcgaagtgga ggccatgtac gccgagaccc gctctgaaac
     1681 cggcctcgcc gccatgtccc ccgcggtgcg cgccgccatg agcaaggtcg agcgccaccg
     1741 cttggtgcag gacgcccacc gcggcgccgc ctaccgcaac cacccgctgc ccatcggcag
     1801 cggccagacc atctcccagc cgtacatcgt tgctctgtcg acggatcttt taggtttaac
     1861 cggcaatagt gtcgtcctgg atgtcggaac gggatccggc taccaggctg ccgtgctcgc
     1921 cgagatcgcc tcgcgtgttt tctccattga gatcattccc tccctcggag aggaagcacg
     1981 caggcgtctg gaaggcctcg gttatggcaa tatcgaagtg aagatcggcg acggctacca
     2041 aggctggccg gagaaggcgc ccttcgacgc catcgtcgtc accgccgccg cgccgcgcgt
     2101 cccgcccgcg ctcgtcgccc aactgaagac cggcggccgc atggtcattc caatcggcgg
     2161 cgaaagcgaa attcagtatc tcaagctcat caccagacgc gccgatggcg gctacgacga
     2221 gaagcgcgtt ctgccggtca ggttcgtccc gctggtgccg ggaaagtaac tagaagcgct
     2281 tgatgagggt catggtgcgg ccctcctggc gcatgtcgag gcggttgagg aagctcatgc
     2341 cgaggagcgc gatgtccagc ccgtcctcga tgacgatgcc ttcgacgttg gtcaattcga
     2401 tgtcgcccag gcgcacgctg gcgagggtca cgcggaaggc ggacgcatcg ccggccgcgg
     2461 tgcgcgtgcg cacggaagcg cccgagcggt agttgatgcc caggcggacg gcgtcggccg
     2521 ccggcagcgc gatggtggtg gcgccggtat cgacgaggaa gcgcatcggg tggccgttga
     2581 tgctgccttc ggagacgaaa tggccgcgcg ggtcggcggc cagggtcacg ctctgcccgg
     2641 aggagacggc cgtgccggcg ccgcggtagt gctgcccgag cgccagcacg cgccgcttgc
     2701 cctcgaactc gacggtagcg cgctcgccct cgaccgaaac aagcgtgacc ccggcgcgcg
     2761 tctgcccgag cttgatggtg cgcggctcgc cgccgtccac cgcgaacacc gccgcgccgc
     2821 tgccgatgag gccgatcagg gcaacgtccg ccgcccgcgc cgcaccgcag gcgaggagga
     2881 cgaaaagcac aataaaatgg cgtggcatga aaccggtcgg catctttcgt tacgcccgca
     2941 ccgaggggcc gggctatttt gccacctatc tcgagcggcg ctcgatcgaa tggcagctga
     3001 tcgcgctcga cgagggcgcc gccgtgccgc gcgatccgcg gcgcttttcc ggcctggtgt
     3061 tcatgggggg tcccatgagc gtgaacgacg agctgccgtg gattgttccc gtgctcgagc
     3121 tgatacgcga agcggttcgc agggacgtag cggtgctcgg ccattgcctc ggcggccagc
     3181 tcatgtccaa ggccttcggc ggcgcggtgc gcgccaaccc ggtgaaggag atcggctggg
     3241 gcgacgtgcg cgtcgccgac aatgcgctcg cgcgcgagtg gctgggcgag ctgcagtcgt
     3301 tcgagtcgtt ccactggcac ggcgagacct tcagcatccc gccgggcgcc acgcgcctgc
     3361 tggagaatgc gcactgcgtc aaccagggct tcgcgctcgg caagcacctc ggcctgcagt
     3421 gccacgtcga gatgaccgag gagctggtgc gcagctggct gaagagcggc gcagatgaga
     3481 tcaaggattc gtcagccagc ccggcggtgc agcagccggc tgagatcgaa agggacctgc
     3541 cggcgcgcct cggcgcgctg cacgaggtcg ccagccgccg gtacgaccgc tggacgaaga
     3601 acctcagtcc cggaaattga tgaattgcag cgggaggtcg gtgacgttcg acctcaccag
     3661 ctggatcgcc gcctgcaggt cgtccttctt cgcgcccgag acgcgcaccg cgtcgccctg
     3721 gatcgagccg gcgaccttca gcttcgcctc cttcagcagc ttgacgatgc tcttcgcctt
     3781 ctcctgggtg atcccgacct ttatcttcat tggtcgtttc agcttatcgc ccgagatctt
     3841 ctcgagagcg ccttcctcga gcgagcgggc gtcgacgctg cgcttggcga ggcgggcgcg
     3901 cagcacgtcc atcacctggc cgagcttgaa ctcgtcgtcg gcgtagaccg agagctcgag
     3961 ctccttcagc tcgatgcggg catcggagcc cttgaagtcg tagcgg
//
LOCUS       DASYJA010000183         2165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1119530, whole genome shotgun sequence.
ACCESSION   DASYJA010000183 DASYJA010000000
VERSION     DASYJA010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119530"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VGO02_03945"
     CDS             complement(<1..328)
                     /locus_tag="VGO02_03945"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEV7476872.1"
                     /translation="MEEALLLARVQFGLNIAFHILFPTITVGLAWLIAWFRLRYHRTG
                     DPAWLETYRLWLKVFALTFAMGVVTGIVMSFQFGTNWPGFMNTVGNIAGPLLAYEVLT
                     AFFLEAT"
     gene            349..1839
                     /locus_tag="VGO02_03950"
     CDS             349..1839
                     /locus_tag="VGO02_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEV7476873.1"
                     /translation="MERPKFDPAREREFTARGWWRDDTLPAWLATHAQRRSRAAALAW
                     QGGTLTWAELQDRVLRAAAGLRRRGVQRGDVVAVQLPNSPEFVISYLAICRLGAAMCT
                     LHMPYRQAEVEALTRHAKARLAVCLPDRKEMFGGTGIAFDDIPDEELLEQNHPHPDAA
                     DVFLLLYTSGTTAAPKGVPHPYRTMLSNARLGAPEHKLDSRDRVLSAAPLSHLYGLYS
                     LHCAWAVGACTVLLPAFSPPELISAMEKLKPTALWTAPAHVAACRAAGLFDGHDWSSL
                     KLAIVSGSAAPPELMRFLKQKLNTCAVTQLWGMTELQAGLYTRPSDPLEVSAASSGRP
                     SPGTEIRLSAENEIQVRGAPLFAGYFDNEPANREAFTADGWFRSGDLGQMDGAGNVAI
                     TGRTKDLINRGGVKFNPAEVEMLLDSHPKILQSALVPMPDPILGEKACVFVTLRSSET
                     VSLEEIVAYLLEKNIAKNKLPEKLVVVAEMPLTPTRKIIKGKLKVP"
     gene            1836..>2165
                     /locus_tag="VGO02_03955"
     CDS             1836..>2165
                     /locus_tag="VGO02_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234157.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEV7476874.1"
                     /translation="MRRVAILGGARIPFARANGAYAQASNQDMLTAALRALVEKFSLK
                     NAKIGEVAAGAVIKHSKDWNLARESALGAGIHPTTPAYDLQRACGTSLSAVAQLANRI
                     ALGEIDSA"
BASE COUNT          385 a          723 c          728 g          329 t
ORIGIN      
        1 acgtcgcctc gaggaagaaa gcggtcagca cctcgtaggc gagcagcggc ccggcgatgt
       61 tgcccaccgt gttcatgaag cccggccagt tggtgccgaa ctggaagctc atgacgatgc
      121 cggtcaccac gcccatggcg aacgtgaggg cgaacacctt gagccacagg cggtaggtct
      181 cgagccaggc ggggtcgccg gtccggtggt agcgcaggcg gaaccaggcg atcagccagg
      241 cgaggccgac ggtgatggtg gggaacagaa tgtggaaggc gatgttcagc ccgaactgga
      301 cgcgggccag gagcagagcc tcttccatgc gtactaatat acgcccggat ggagcgaccg
      361 aagttcgacc cggcgcgcga gcgcgaattc accgcgcgcg gctggtggcg cgacgacacc
      421 cttcccgcct ggctcgccac ccacgcgcag cgccgctcgc gggcggcggc tctcgcctgg
      481 cagggcggca cgctcacctg ggccgagctg caggaccggg tgctgcgcgc cgccgccggc
      541 ctcaggcgcc gcggcgtgca gcgcggcgat gtggtcgcgg tgcagctgcc caactcgccg
      601 gaatttgtca tttcgtacct ggccatctgc cgcctgggcg ccgcgatgtg cacgctgcac
      661 atgccctacc gccaggccga ggtcgaggcg ctgacgcgcc acgccaaggc gcgcctcgcc
      721 gtctgcctgc cggacagaaa ggaaatgttt ggcgggacgg gtattgcctt tgacgacatt
      781 ccagacgagg aattgctcga gcaaaatcac ccccacccgg acgcggccga cgtgttcctc
      841 ctgctctaca cctcggggac gaccgcggcg ccgaagggcg tgccgcatcc gtaccgcacc
      901 atgctctcca acgcgcgcct gggcgcgccg gagcacaagc tcgattcgcg cgaccgcgtg
      961 ctgagcgcgg cgccgctgtc gcacctctac ggcctgtatt cgctgcactg cgcctgggca
     1021 gtcggcgcgt gcacggtcct gctacccgcc ttttcgcctc ctgaattgat ttctgcaatg
     1081 gaaaaactga agccgactgc gttgtggacc gcgcccgccc acgtcgccgc ctgccgcgcc
     1141 gccggcctgt tcgacgggca cgactggtcc tcgctcaagc tggcgatcgt ctccggctca
     1201 gccgcgccgc ctgagttgat gagatttctg aaacagaagc tgaacacctg cgcggtcacg
     1261 cagctgtggg gcatgaccga gctgcaggcc gggctctaca cgcggccgtc ggacccgctc
     1321 gaggtgtcgg cggcgagctc gggccggccg agcccgggaa cggaaatacg cctttccgca
     1381 gaaaatgaaa tccaggtaag gggcgcgccg ctgttcgccg gctacttcga caacgaaccg
     1441 gcgaaccgcg aggcgttcac cgccgacggc tggttccgca gcggcgacct cggccagatg
     1501 gacggcgccg gcaacgtcgc catcaccggg cgcaccaagg acctgatcaa ccgcggcggc
     1561 gtcaagttca acccggccga ggtcgagatg ctgctcgact cgcatccgaa gatcctgcag
     1621 tcggcgctcg tgccgatgcc cgacccgatc ctcggggaga aggcttgcgt atttgtgacg
     1681 ttaagaagtt cagaaacggt ttctctcgag gaaatcgtcg cctacctgct tgaaaagaac
     1741 atcgccaaga acaaactgcc cgagaagctg gtcgtcgtcg ccgagatgcc gctcaccccg
     1801 acccgcaaga tcatcaaagg aaagctgaag gtgccatgag acgcgttgcg attctgggtg
     1861 gggcgaggat tccgttcgcg agggcgaacg gggcgtatgc gcaggcgagc aaccaggaca
     1921 tgctgaccgc tgccctgcgg gcgctggtcg agaagttttc cctgaagaat gcaaagatcg
     1981 gcgaggtggc ggcgggggcg gtgatcaagc attcgaagga ctggaacctg gcgcgcgagt
     2041 cggcgctcgg cgccggcatc catccgacga cgccggccta cgacctgcag cgcgcctgcg
     2101 gcaccagcct ctccgcggtg gcgcagctcg ccaaccgcat cgcgctgggc gagatcgact
     2161 cggcc
//
LOCUS       DASYJA010000184         1727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_573851, whole genome shotgun sequence.
ACCESSION   DASYJA010000184 DASYJA010000000
VERSION     DASYJA010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573851"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VGO02_03960"
     CDS             complement(<1..486)
                     /locus_tag="VGO02_03960"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEV7476875.1"
                     /translation="MLDTTRRVATPEGIELTLRLAGPVPRAIAWAIDLAIRAALLFAV
                     SMLAAALGKAGMGVILLTAFFVEWLLPAWFEAVWNGQTPGKRAMGIAVLNDDGTPLRW
                     PGALTRNLLRAVDFLPLLYGVGLVAMLANRDFKRLGDLAAGTVVVYREEKLAHAAVIP
                     AA"
     gene            complement(522..1715)
                     /gene="rho"
                     /locus_tag="VGO02_03965"
     CDS             complement(522..1715)
                     /gene="rho"
                     /locus_tag="VGO02_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEV7476876.1"
                     /translation="MEGANRMRKHDLIFALLKQQAKKGESIFGGGVLEVLPDGFGFLR
                     SPETSYLASTDDIYVSPSQIRRFNLHTGDTIEGEIRTPKDGERYFALVKVDKVNGEAP
                     EAAKNKILFENLTPLHPDKAFKLEREIKAEENMTGRVLDIVAPIGKGQRGLIVSPPKA
                     GKTVMLQHIAHALVANHPEISLIVLLIDERPEEVTEMSRSVRGEVVASTFDEPASRHV
                     QVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVVPASGKVLTGGVDANALQKPK
                     RFFGAARNVEEGGSLTIIATALIDTGSRMDDVIYEEFKGTGNMEVHLDRRMHEKRIYP
                     AINVNRSGTRREELLIEQPLLQKVWVLRKLLFPMDELEAAEFLIDKLRATKTNAEFFD
                     SMRRG"
BASE COUNT          252 a          558 c          600 g          317 t
ORIGIN      
        1 cgccgccgga atcaccgccg cgtgcgcgag cttctcctcg cggtagacca ccaccgtgcc
       61 ggcggcgagg tcgcccaggc gcttgaagtc gcggttggcg agcatggcga cgagcccgac
      121 gccgtaaagg agcggcagga agtcgaccgc gcgcagcagg ttgcgggtga gggcgccggg
      181 ccagcgcagc ggcgtgccgt cgtcgttcag cacggcgatg cccatggcgc gcttgcccgg
      241 ggtctggccg ttccagaccg cctcgaacca ggccggcagc agccattcga cgaagaaggc
      301 ggtgagcagg atcacgccca tgccggcctt gcccagggcg gcggcgagca tcgacacggc
      361 gaacagcagt gcggcgcgga tggcgaggtc gatcgcccag gcgatggcgc gcggcaccgg
      421 gccggcgagg cgcagcgtga gctcgattcc ttcgggtgta gcgactcgac gcgtggtgtc
      481 gagcatgcgg ctcccctccc cagggaaacc gattctagtt cttatccgcg ccgcatcgag
      541 tcgaagaact cggcgttggt cttggtggcg cgcagcttgt cgatgaggaa ttccgccgct
      601 tcgagctcgt ccatcgggaa caggagcttg cgcagcaccc agaccttctg cagcagcggc
      661 tgctcgatca gcagctcctc gcgccgcgtg ccggaccggt tcacgttgat ggcggggtag
      721 atgcgcttct cgtgcatgcg ccggtcgagg tgcacctcca tgttgccggt gcccttgaat
      781 tcctcgtaga tcacgtcgtc catccggctg ccggtgtcga tcagcgcggt ggcgatgatg
      841 gtgagcgagc cgccttcctc gacgttgcgc gccgcgccga agaagcgctt gggcttctgc
      901 agcgcgttgg cgtcgacgcc gccggtgagc accttgcccg aagcgggaac cacggtgttg
      961 taggcgcgtg ccaggcgggt gatggagtcc agcaggatga cgacgtcctt tttatgctcg
     1021 accaggcgtt tcgccttctc gatcaccatc tcggcgacct gcacgtggcg cgaggccggc
     1081 tcgtcgaagg tggaggccac cacttcgccg cgcaccgagc gcgacatctc ggtgacttcc
     1141 tcgggccgct cgtcgatcag cagcacgatc agggaaatct cggggtggtt cgccaccagg
     1201 gcgtgggcga tgtgctgcag catcaccgtc ttgccggcct tgggcgggct gacgatcagg
     1261 ccgcgctggc ccttgccgat cggcgccacg atgtcgagca cccggccggt catgttctcc
     1321 tcggccttga tctcgcgctc gagcttgaac gccttgtccg ggtgcagcgg cgtcaggttc
     1381 tcgaacagga tcttgttctt ggccgcctcc ggcgcttcgc cgttgacctt gtcgaccttg
     1441 accagcgcga agtatcgctc gccgtccttc ggcgtgcgaa tctcgccctc gatggtgtcg
     1501 ccggtgtgca ggttgaagcg gcggatctgc gaaggcgaga cgtaaatgtc gtcggtgctc
     1561 gcgaggtagg aggtctcggg cgagcgcagg aagccgaagc cgtcgggcag tacttcgagg
     1621 acgccgcccc cgaagatcga ctctcccttc ttcgcctgct gcttcagcag cgcgaagatg
     1681 aggtcgtgct tgcgcatccg gttggcgcct tcgatctcgt tcgcgtt
//
LOCUS       DASYJA010000185         7133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2845141, whole genome shotgun sequence.
ACCESSION   DASYJA010000185 DASYJA010000000
VERSION     DASYJA010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7133
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2845141"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VGO02_03970"
     CDS             complement(<1..412)
                     /locus_tag="VGO02_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEV7476877.1"
                     /translation="MALLQIAEPGQSPAPHEHRLAVGIDLGTTNSLVATVRSGMPVVL
                     PDSLGRPLLPSVVRYHADGKIEVGYAAQAKQAVDPKNTIISVKRFMGRGLKDVAHIET
                     APYDFVDAPGMVQMRTAAGVKSPVEVSAEILKVLR"
     gene            complement(412..927)
                     /gene="hscB"
                     /locus_tag="VGO02_03975"
     CDS             complement(412..927)
                     /gene="hscB"
                     /locus_tag="VGO02_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEV7476878.1"
                     /translation="MQNHFELFGLSPAFVLDKEVLEKAYREIQSKVHPDRFAHAGDAE
                     RRASLQWTTRVNEAYRALKSPVQRAKHILELHGIDVAFETNTQMPTDFLLRQLELREE
                     LEAAVQKKDPALLDALRRTISGEKLLLENQIGDAIDSKKDYQGSAGPVRKLMFLDRLD
                     SEIDSAYEEIE"
     gene            complement(932..1255)
                     /gene="iscA"
                     /locus_tag="VGO02_03980"
     CDS             complement(932..1255)
                     /gene="iscA"
                     /locus_tag="VGO02_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEV7476879.1"
                     /translation="MAITLTEKAANHVQSFLAKRGKGVGLRLGVRTSGCSGMAYKLEF
                     VDAPGPDDVQFESHGVKVVVDPKSLPYIDGTELDYTREGLNEGFKFKNPKVKDECGCG
                     ESFNV"
     gene            complement(1255..1635)
                     /gene="iscU"
                     /locus_tag="VGO02_03985"
     CDS             complement(1255..1635)
                     /gene="iscU"
                     /locus_tag="VGO02_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEV7476880.1"
                     /translation="MAYSDKVIDHYENPRNVGSFEKGDETVGTGMVGAPACGDVMKLQ
                     IKVGADGRIEDARFKTYGCGSAIASSSLVTEWVKGKTLDEAATIKNTQIAEELSLPPV
                     KIHCSILAEDAIKAAVEDYKKKRK"
     gene            complement(1645..2853)
                     /locus_tag="VGO02_03990"
     CDS             complement(1645..2853)
                     /locus_tag="VGO02_03990"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEV7476881.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMIPYLTEFFGNAASRSHAFGWK
                     AEEAVEEARGHVAALIGADPKEIVWTSGATEGNNLAIKGAANFYKTKGKHIITQKTEH
                     KAVLDTVRELERQGFEATYLEVQQNGLVDVEQFKAAIRPDTILASIMMVNNEIGVIQP
                     VEQIAEICRGKGIIFHCDAVQGAGRVDIDLQRFKADLLTITAHKIYGPKGIGALYIRR
                     KPRVRIEAQIHGGGHERGFRSGTLPTHQIVGFGEAARLAKLEMAEENRRIGALRDRLW
                     DKVKDMEEVYVNGDLERRIPHNLNVSFNFVEGESLIMAVKDIAVSSGSACTSASLEPS
                     YVLRALGRSDELAHSSVRLTLGRFTTEQEVDFAADLLRRKVEKLRELSPLWEMYKDGV
                     DLNSVQWAAH"
     gene            complement(2850..3332)
                     /gene="iscR"
                     /locus_tag="VGO02_03995"
     CDS             complement(2850..3332)
                     /gene="iscR"
                     /locus_tag="VGO02_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEV7476882.1"
                     /translation="MRLTTKGRFAVTAMVDLAMRQTRGPVTLAAISERQHISLSYLEQ
                     LFGKLRRAKLVSSVRGPGGGYNLAQPPQGISVASIVTAVDEPLDATQCGGKENCHDDK
                     RCMTHDLWATLNEKMQEYLSSVTLADLVAHQAGKPVAVIKHFRMERTEEKPAAEKVAA
                     "
     gene            complement(3395..4096)
                     /gene="cysE"
                     /locus_tag="VGO02_04000"
     CDS             complement(3395..4096)
                     /gene="cysE"
                     /locus_tag="VGO02_04000"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEV7476883.1"
                     /translation="MFRKLRNDIGCILERDPAARSGWEVLTCYPGLHAIYIHKLAHWF
                     WRARLRWLGRFTSQLGRWLTGIEIHPGAKIGCCVFIDHGMGVVIGETAEVGDGCTIYQ
                     GVTLGGTSLYRGAKRHPTLGEGVVVGAGAKVLGGFTVGDGARIGSNAVIVKEVPAGAT
                     VVGIPGRIVEDHAAVAERFAAYAVVQENDDPYAKAIQKLVEHSQELEKALLALNEKLA
                     GIEKDKQKEQLRAVK"
     gene            complement(4132..4830)
                     /locus_tag="VGO02_04005"
     CDS             complement(4132..4830)
                     /locus_tag="VGO02_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV7476884.1"
                     /translation="MNDIRIVLCRPSHPGNIGAAARAMKAMGLTDLRLVAPERWPAKE
                     DQWMATHAADVLQDAVVHEKLEQAISDCAAAFALSARPREWSPQVLDVRAAAARAAQV
                     GDKVAFVFGSEDAGLTNEEMFACQFLVHIPANPAFSSLNLAQAVQIVCYELFLLRGEA
                     VPKARVEKLATVEDIEGLYAHLEQAAVKSGFYVPQTRSKLPERLRRLFSRVPQLEREE
                     VNILRGLLKALLGK"
     gene            4858..5631
                     /locus_tag="VGO02_04010"
     CDS             4858..5631
                     /locus_tag="VGO02_04010"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV7476885.1"
                     /translation="MHPMLNIAVRAARKAGSIINRAALSSEGLKVKAKQANDFVTQVD
                     QAAEEAIIGIVRHAYPEHGILAEESGRSEGAAEYVWIIDPLDGTTNFIHGFPQYCVSI
                     GVQHRGALAHAVVYDPLKNELFTASQGRGAFLNDRRIRVSKCLRLQDALVGTGFPFRE
                     LTRIDLYIGQLKKLMSGSSGVRRAGAAALDLAYVACGRLDAFWELGLSPWDMAAGALL
                     IQEAGGLVGDLGGEAGYLERGEIVAASPKIFPALLQTLK"
     gene            5631..6521
                     /locus_tag="VGO02_04015"
     CDS             5631..6521
                     /locus_tag="VGO02_04015"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEV7476886.1"
                     /translation="MLARLLAWLKWSRVESASYANRVGARQYKVYRPWRGAGRTRPLI
                     VMLHGCNQTADDFAAGTRMRQLAARMGCIVAYVEQSAAAHPSRCWNWFKAAHQEREEG
                     EPSLIAGITREVIARHNADPRRVYVAGFSAGGAMAAIMGHTYSDLYAAVGIHSGLPYA
                     AARDAVSALAAMRGLRGTLDPRYRPAAFRAIPTIVFHGDLDTTVHSRNSLQAIMQAGP
                     AADDRGTDAVEKGEENGRRYTRTVHSDASGRTAVEHWLVHGSGHAWSGGSSLGSFTDR
                     RGPDASSAMLKFFLDFTRAA"
     gene            6587..6916
                     /locus_tag="VGO02_04020"
     CDS             6587..6916
                     /locus_tag="VGO02_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEV7476887.1"
                     /translation="MLIVAPALTVGGGAFTSLHPEEAYPDPQVPADFDPYAGLVSQVQ
                     ARLRALGFDAGPINGDFGSKTQAALAQFQLSAGVAASGQLDDATLAELGVERKGQAAA
                     GSTSPSR"
     gene            complement(6913..>7133)
                     /locus_tag="VGO02_04025"
     CDS             complement(6913..>7133)
                     /locus_tag="VGO02_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEV7476888.1"
                     /translation="TGGEFVMTEQRPRMQSRAEVVPLQQGDAVVFAVHNRPVQGTRGV
                     HRVNLRHGVSRVRSGRRHTAGIIFHDAA"
BASE COUNT         1155 a         2395 c         2278 g         1305 t
ORIGIN      
        1 cccttaggac cttcaggatt tctgcggaaa cctccaccgg gctcttcacg cccgcggcgg
       61 tgcgcatctg caccatgccc ggggcgtcga caaaatcgta gggcgcggtc tctatgtgcg
      121 caacatcctt taggccgcgg cccatgaagc gcttgaccga gatgatggtg ttcttcggat
      181 ccaccgcctg cttcgcctgc gccgcgtagc cgacctcaat cttgccgtcg gcgtggtagc
      241 gcacgacgga gggcagcagc ggccggccga gcgaatcggg cagcaccacc ggcatcccgc
      301 tgcgcacggt ggcgaccagc gagttggtgg tgccaaggtc gatgcccact gcgagccggt
      361 gctcgtgcgg cgccggcgac tgacctggtt cggcgatctg cagcagagcc actactcgat
      421 ctcctcgtaa gcggagtcga tttcagaatc caatctatcc agaaacatca gtttccgcac
      481 cggccctgcg gacccttgat agtccttctt ggaatcgatc gcgtcgccaa tctggttttc
      541 gagaagaagc ttttcaccag agatcgttct gcgcaaagcg tccagcaatg caggatcctt
      601 cttctggacc gcagcttcca gctcctctcg cagctcaagc tgccgaagca ggaaatcggt
      661 tggcatctgg gtattggttt cgaaggcgac gtcgatgccg tgcagctcga ggatatgctt
      721 cgcgcgctgc accgggctct tcagcgcgcg gtaagcctcg ttcacgcgcg tcgtccactg
      781 cagcgaggcg cgccgctcgg cgtcgccggc gtgcgcgaag cggtcggggt gcaccttgga
      841 ctggatctcg cggtaggcct tctccagaac ttccttgtcc agcacgaagg cgggagacag
      901 cccgaacagt tcgaagtggt tctgcaacct gctagacgtt aaagctttcc ccgcacccgc
      961 attcgtcctt gaccttgggg ttcttgaact tgaacccctc gttgaggcct tcgcgggtgt
     1021 agtcgagctc ggtgccgtcg atgtacggca ggctcttggg atcgaccacg accttgacgc
     1081 cgtggctctc gaactgcacg tcatcggggc ccggcgcgtc gacgaactcg agcttgtagg
     1141 ccatccccga gcagcccgag gtgcgcacgc caaggcgcag gccgacgccc ttgccgcgct
     1201 tggcgaggaa ggactggacg tggttcgcgg ccttctcggt gagggtgatc gccattactt
     1261 gcgctttttc ttgtagtcct cgaccgccgc cttgatcgcg tcctcggcga ggatcgagca
     1321 gtggatcttg accggcggga gcgacagctc ctcggcgatc tgcgtgttct tgatggtcgc
     1381 cgcctcgtcg agagtcttgc ccttcaccca ctcggtgacc agggagctcg aggcaatggc
     1441 cgagccgcag ccgtaggtct tgaagcgcgc gtcctcgatg cggccgtcgg cgccgacctt
     1501 gatctgcagc ttcatcacgt cgccgcaggc cggcgcgccg accatgccgg tgccgacggt
     1561 ctcgtcgcct ttttcgaacg aacccacgtt cctcgggttc tcgtagtggt cgatgacttt
     1621 atcgctgtat gccatagccg ttcctcagtg tgccgcccat tggacggagt ttagatcaac
     1681 gccgtccttg tacatttccc aaagcggaga caactccctt agcttctcca ccttgcgccg
     1741 gagcaggtcc gccgcaaagt cgacttcctg ctcggtggtg aagcgcccga gcgtgagacg
     1801 caccgagctg tgcgccagct cgtcggagcg gcccagcgcc cgcagcacat aggagggctc
     1861 gagcgaggcc gaagtgcagg ccgagcccga cgatacggcg atgtccttca ccgccatgat
     1921 cagcgactcg ccctcgacga agttgaagct gacgttgagg ttgtgcggga tgcggcgctc
     1981 gaggtcgccg ttcacgtaca cctcttccat gtccttgacc ttgtcccaca gccggtcgcg
     2041 cagcgcgccg atgcggcggt tttcctcggc catctcgagc tttgccaggc gcgccgcctc
     2101 gccgaagccg acgatctggt gcgtcggcag cgtgccggag cgaaagcccc gctcgtggcc
     2161 gccgccgtgg atctgcgcct cgatgcgcac gcgcggcttc ctgcggatgt agagcgcgcc
     2221 gatgcccttg gggccgtaga tcttgtgcgc ggtgatggtc agcaggtcgg ccttgaatct
     2281 ctgcaggtcg atgtcgaccc ggccggcgcc ctgcaccgca tcgcaatgga agatgatgcc
     2341 cttgccgcgg cagatctcgg cgatctgctc gaccggctgg atcacgccga tctcgttgtt
     2401 caccatcatg atcgaggcga ggatggtgtc cgggcggatc gccgccttga actgctcgac
     2461 gtcgaccagg ccgttctgct gcacttcgag gtacgtcgcc tcgaagccct ggcgctcgag
     2521 ctcgcgcacc gtgtcgagca ccgccttgtg ctcggtcttc tgcgtgatga tgtgcttgcc
     2581 cttggtcttg tagaagttcg ccgcgccctt gatggcgagg ttgttgccct cggtggcgcc
     2641 ggaggtccag acgatctcct tcgggtcggc gccgatcagc gccgcgacgt ggccgcgcgc
     2701 ctcctcgacc gcctcttccg ccttccagcc aaacgcgtgc gagcggctcg ccgcgttgcc
     2761 gaagaactcg gtgaggtagg ggatcatctt ctcggccacg cgcggatcga ccggcgtagt
     2821 cgcggagtaa tccaggtaga tcggcaactt catgccgcaa ctttctccgc tgcgggtttt
     2881 tcctccgtcc gctccatgcg gaagtgcttg attaccgcga cgggcttgcc ggcctggtgc
     2941 gcgaccaggt ccgccagcgt caccgacgac aggtattcct gcatcttctc gttgagcgtc
     3001 gcccagaggt cgtgagtcat gcaacgcttg tcgtcgtggc agttctcctt gccgccgcat
     3061 tgcgtcgcgt cgagcggctc gtcgaccgcc gtcacgatgc tggcgaccga gatgccctgc
     3121 gggggttgcg cgaggttgta gccgccgccg ggaccgcgca cgctggaaac cagcttcgcg
     3181 cggcgcagct tgccgaacaa ctgctccaga taggacagag aaatgtgctg gcgctcgctg
     3241 atggcggcga gcgtaaccgg cccgcgcgtc tggcgcatcg ccagatcgac cattgcggta
     3301 accgcgaacc tgcctttggt agtgagtctc atggggttcc taatacctga ttaatacgct
     3361 caagtatacc taaacccgag cgattcagtc aaccttactt gacagctcta agctgttctt
     3421 tctgcttgtc tttttctatg ccagccagct tctcgttgag ggccaggagg gctttctcga
     3481 gctcctgcga atgctcgacc agcttctgga tcgccttggc ataagggtca tcgttttcct
     3541 gcaccactgc gtatgcagca aagcgctcgg caacagccgc gtgatcctcg acgatgcgcc
     3601 cggggatgcc gaccaccgtt gccccggcgg gcacttcctt cacgatcacc gcgttcgagc
     3661 cgatgcgcgc gccatcgcct acggtgaagc cgccgagcac cttggcgccg gcccccacta
     3721 ccaccccctc gccgagcgtc gggtggcgtt tcgcgccgcg gtagagcgag gtgccgccca
     3781 gcgtcacgcc ctggtaaatg gtgcaaccgt cgccgacttc cgcggtttcg ccgatcacca
     3841 cgcccatgcc gtggtcgata aacacgcagc agccgatttt tgccccgggg tggatctcga
     3901 tcccggtcag ccagcgcccg agctgcgagg tgaagcgacc gagccagcgc aggcgcgcgc
     3961 gccagaacca gtgggcgagc ttgtggatgt agatcgcatg caggccggga tagcaggtca
     4021 gcacttccca gccggagcgc gccgccgggt cgcgctcgag gatgcagccg atgtcgttgc
     4081 gcagtttcct gaacatcaag cgattctaga gttcccgact gatttgatca actatttgcc
     4141 aagcaacgcc ttgaggagcc cgcggaggat attcacctcc tcgcgctcga gctgcggcac
     4201 gcgcgaaaac agccgccgca gccgctcggg cagcttggag cgggtttgcg gaacatagaa
     4261 gccgctcttc accgccgctt gttccaagtg tgcatacagt ccctcgatat cttcaaccgt
     4321 ggcgagcttt tccaccctcg ccttgggaac ggcttccccc cgcagaagga aaagctcgta
     4381 gcacacgatc tgcaccgcct gggcgagatt gagcgagctg aaggccgggt tggccggaat
     4441 gtggaccagg aactggcagg cgaacatctc ctcgttggtg aggccagcgt cctcgctgcc
     4501 gaagacgaac gccaccttgt cgccgacctg tgccgcgcgg gccgcagcag cgcgcacgtc
     4561 gagaacctgc ggcgaccact cccgcggccg cgccgacagc gcgaaggcag cggcgcagtc
     4621 gcttatcgcc tgctctagct tttcgtgaac caccgcgtct tgcaggacat ccgcggcgtg
     4681 cgtggccatc cactggtcct cctttgccgg ccagcgttcc ggggcgacga gacgcaggtc
     4741 cgtcaggccc atcgccttca tggcgcgcgc tgcggcgccg atgttccccg ggtggctggg
     4801 cctgcaaagg acgatgcgga tgtcgttcac ccctagagtc tagaatacgg ccccttcatg
     4861 caccccatgc tcaatatcgc cgtgcgcgcc gcgcgcaagg ccgggtcgat catcaaccgc
     4921 gccgcgctca gcagcgaagg gctgaaggtc aaggcaaagc aggccaacga tttcgtcacc
     4981 caggtcgacc aggcggccga ggaggcgatc atcggcatcg tgcggcacgc ctaccccgag
     5041 cacggcatcc tcgccgagga atccggccgc agcgagggcg cggccgagta tgtctggatc
     5101 atcgacccgc tggacggcac caccaacttc atccacggct tcccgcagta ctgcgtttcg
     5161 atcggcgtgc agcaccgcgg cgccctcgcc cacgcggtgg tctacgaccc gctgaagaac
     5221 gagctgttca ccgcttccca ggggcgcggc gcgttcctca acgaccggcg catccgggtc
     5281 tcgaaatgcc tgcggctgca ggatgcgctg gtcggcaccg gctttccgtt ccgcgagctc
     5341 acgcgcatcg acctgtacat cggccagctg aagaagctga tgtcgggcag cagcggggtg
     5401 cgccgggcgg gcgcggccgc gctcgacctc gcctacgtcg cctgcggccg gctcgacgcg
     5461 ttctgggagc tcggcctgtc gccctgggac atggcggcag gcgcgctgct catccaggaa
     5521 gccggcgggc tggtaggcga cctcggcggc gaagcgggct atctggagcg gggcgagatc
     5581 gtagccgcat cgcccaagat cttccccgcc ctgctgcaaa cgctcaagta gtgctcgccc
     5641 ggctcctcgc atggctgaaa tggagccgcg tcgagtccgc gagctacgcc aaccgcgtcg
     5701 gcgcgcgcca atacaaggtc tatcgtccct ggcggggcgc cggaaggacc cggccgctga
     5761 tcgtcatgct gcacggctgc aaccagaccg cggacgattt cgcggcggga acgcgcatga
     5821 ggcagctggc cgcgcgcatg gggtgcatcg ttgcctacgt ggaacagtcc gcggcggcac
     5881 atccctcgcg gtgctggaac tggttcaagg cggcgcacca ggagcgcgaa gaaggcgagc
     5941 cttcgctcat cgcagggatc acgcgtgagg tgatcgcccg gcacaacgcc gatccccggc
     6001 gcgtatacgt cgccggcttt tcggcaggag gcgcgatggc ggcgatcatg ggccacacct
     6061 attccgatct gtatgccgcg gtcggcatcc actcgggcct gccctatgcc gccgcccgcg
     6121 acgcggtgtc ggcgctagcc gcgatgcgcg gactgcgcgg cacgctcgat ccgagatacc
     6181 ggccagcggc ttttcgtgcc attccaacca tcgtgtttca tggcgacctg gacacgaccg
     6241 tccactcgcg aaacagcctg caggcgatca tgcaggccgg ccccgcagcg gacgatcggg
     6301 gaacggatgc ggtggagaag ggcgaggaaa acggccggcg ttacacgcgc accgtccact
     6361 cggacgcgtc gggcagaacg gcggtcgaac actggctggt gcacggcagc ggtcacgcat
     6421 ggtccggcgg aagcagcctc ggctcgttca ccgaccggcg cggacccgac gcaagctccg
     6481 cgatgctcaa attcttcctc gacttcaccc gggcggccta gacctcgccg ctgggtacgg
     6541 cgcttgctgc cggctcgcca tggactggcg cgcagtcgca gccgccgtgc tgatcgtggc
     6601 cccggcacta accgtaggcg gcggagcgtt tacctccctg catcccgaag aggcctaccc
     6661 ggacccgcag gttcccgccg atttcgatcc ctatgccggc ttggtgagcc aggtgcaggc
     6721 gaggctacgc gccctgggat tcgacgccgg cccgatcaac ggcgactttg gctccaagac
     6781 gcaggccgcg ctggcgcaat tccagctttc cgccggcgtg gctgccagcg gacagctgga
     6841 cgatgcgacg ctggcggagc taggtgtgga gcgcaaaggc caggcggctg caggctctac
     6901 cagtcccagc cgttaagccg cgtcgtgaaa gatgatgcca gcggtatgcc gccggcctga
     6961 acgaacccga ctgacgccgt gccgcaggtt gacgcggtgg acgccgcgcg tgccctgaac
     7021 cggccggttg tgcaccgcaa acacgaccgc atccccctgc tgcaacggca cgacttcggc
     7081 gcgcgactgc atgcggggac gctgctcggt catgacgaac tcgcctcccg tga
//
LOCUS       DASYJA010000186         3156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2026441, whole genome shotgun sequence.
ACCESSION   DASYJA010000186 DASYJA010000000
VERSION     DASYJA010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2026441"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VGO02_04030"
     CDS             complement(<1..604)
                     /locus_tag="VGO02_04030"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEV7476889.1"
                     /translation="MDRRATEGTLLIELGGRPVECRFARRRRRTLGITVDADGLRVAA
                     PLRAPWRDIEAFLRDKERWILTKLEEWARMPRPRVLRGASGESLPYLGVEHTLEVRDG
                     GRAVARDGATITVSAPHSHVLGTLLGWLKANALETLAPRVACYAAALAVPAPRVALSS
                     GRTQWGVCAADGTIRLNWRLVHLAPALADYVAAHEAAHLVE"
     gene            complement(571..1275)
                     /locus_tag="VGO02_04035"
     CDS             complement(571..1275)
                     /locus_tag="VGO02_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV7476890.1"
                     /translation="MTAVRSSLFVLALVLVTPPYALLALATLPLPRMARYRIISGWSR
                     LVIILAKLILRIQWRVEGREHLPQRPAVILAKHQSAWETMAFQLIFPPQVHVLKRELL
                     WIPFFGWGLALMSPIAIDRRRGLAALRAMSKKGAQRLGQGFWVVVFPEGTRVAPGERR
                     AYQPGGAWLAAASGAPVVPVAHNAGLLWPRYAFLKHPGTITVRIGPAIDPANRDPATI
                     NRLAEAWIEEQQKALC"
     gene            complement(1272..1805)
                     /gene="gmhB"
                     /locus_tag="VGO02_04040"
     CDS             complement(1272..1805)
                     /gene="gmhB"
                     /locus_tag="VGO02_04040"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEV7476891.1"
                     /translation="MKLVILDRDGTINHDSDQYIKSPEEWRPIDGSLEAIARLTQADY
                     RIVVATNQSGIARGLFDTTTLIAIHDMLQRALGQMGGHIDAFFFCPHAADSACECRKP
                     KPGMLLEIASRFNVSLDGVYMVGDAQRDLEAAAAAGAKPVLVLTGKGKKTQKEGKLPP
                     GTVVFADLAAFAEHIAP"
     gene            complement(1816..>3156)
                     /gene="glyS"
                     /locus_tag="VGO02_04045"
     CDS             complement(1816..>3156)
                     /gene="glyS"
                     /locus_tag="VGO02_04045"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEV7476892.1"
                     /translation="GEYQALLDEVAALVEFPAIYAGAFEEAFMEVPQECLILTMRQNQ
                     KYFPLFGAGGKLLPRFLIVSNMKVAEPRHIIGGNERVVRPRLEDARFFYNQDRKARLE
                     TRVPQLAKVVYHSKLGSQLERVQRIKLLAGGIARALKADPLIAERAAELCKADLLTGM
                     VGEFPELQGIMGRYYALHDGEPAAVADAIESHYRPRFAGDRLPEGSIACAVALADKLD
                     TLAGLFSIGEQPTGEKDPFGLRRAALGVIRIIVERKLPLPLNELASAAFEGHRAKAST
                     DLIEFLFARFEGYLKEQGFSTLQVDSVLSQRPVQLGVVPQQLEAVKAFQALPEAESLA
                     AANKRVANILRQAAEKGESFANAERSALKEAAEIALFDALQKASGSANQLFERGDYAG
                     YLKTFAVLKAPVDAFFDKVMVMAEQKALRRNRLALLADLRKAMNRVADISKLAV"
BASE COUNT          474 a         1079 c         1111 g          492 t
ORIGIN      
        1 tctcgacgag gtgcgccgcc tcgtgcgccg cgacgtagtc ggcgagcgcg ggggcaaggt
       61 gcaccaggcg ccagttgagc cggattgtgc cgtcggcggc gcacacgccc cactgcgtgc
      121 gtccgctgga aagtgccaca cgcggcgccg gcacggcgag cgccgcggcg tagcaagcga
      181 cgcgcggcgc gagcgtctcg agcgcgttcg ccttcagcca gccgaggagc gtgccgagca
      241 cgtgcgagtg cggcgcggac acggtgatcg tcgcgccgtc gcgggccacc gcgcggccgc
      301 cgtcgcgcac ctcgagggtg tgctcgaccc cgaggtaggg cagcgattcg ccgctcgcgc
      361 cgcgcagcac gcgcggtcgc ggcatccgcg cccactcctc gagcttagtc aggatccagc
      421 gctccttgtc gcgcaggaag gcctcgatat cacgccacgg cgcgcgtaac ggcgcggcga
      481 cgcgcagccc gtcggcgtcc accgtgatac cgagggtgcg ccgtcggcgc cgcgcgaaac
      541 ggcattcgac cgggcgcccg cccagctcga tcagcagagt gccttctgtt gctcttctat
      601 ccatgcttca gcgaggcggt tgatggtggc cgggtcgcga ttcgccgggt cgatcgcggg
      661 cccgatgcgc acggtgatgg tgccggggtg cttgaggaag gcgtagcgcg gccagagcag
      721 ccccgcgttg tgcgcgaccg gcaccaccgg agcgccggag gcggcggcga gccaggcgcc
      781 gccgggctgg taggcgcgcc gctcgccggg cgcgacgcgc gtgccttccg ggaacaccac
      841 cacccagaag ccctggccca ggcgctgcgc gcccttcttc gacatggcgc gcagcgccgc
      901 gagcccgcgc ctgcggtcga tggcgatcgg gctcatcagc gcgagccccc agccgaagaa
      961 cggaatccag aggagctcgc gcttcagcac gtgcacctgc ggcgggaaga tgagctggaa
     1021 ggccatcgtc tcccacgccg actggtgctt ggccaggatg acggccggac gctgcggcag
     1081 gtgctcgcgg ccttcgacgc gccactggat gcgcaggatc agcttcgcca ggatgatcac
     1141 caggcgcgac catcccgaga tgatccggta gcgcgccatg cgcggcagcg gcagcgtggc
     1201 cagcgcaagc agcgcgtagg gcggcgtgac caggacgagc gcgagcacga acaggctcga
     1261 gcgcacggcc gtcatggagc gatgtgctcg gcgaacgcgg ccaggtcggc gaacaccacg
     1321 gttccggggg gcagcttgcc ttccttctgc gtcttcttgc ccttgccggt cagcaccagc
     1381 accggcttgg cgccggctgc cgcagcggct tcgaggtcgc gctgcgcgtc gcccaccatg
     1441 tagacgccgt cgagggagac gttgaagcgg ctcgcgatct cgagcagcat ccccggcttc
     1501 ggcttgcggc actcgcaggc ggagtccgcg gcgtgcgggc agaagaagaa tgcgtcgatg
     1561 tgcccgccca tctgcccgag cgcacgctgc agcatgtcgt ggatcgcgat gagcgtcgtg
     1621 gtgtcgaaca gcccgcgcgc gatgcccgac tggttggtgg cgaccacgat gcggtagtcg
     1681 gcctgcgtca gccgcgcgat cgcctccagg ctgccgtcta tcggccgcca ttcctcgggc
     1741 gacttgatgt actggtcgct gtcgtggttg atggtgccgt cgcggtcgag gataacgagt
     1801 ttcatagcca gcggttcaca cggccagttt cgagatgtcg gcgacgcggt tcatggcctt
     1861 gcgcaggtcg gcgagcaggg ccagccgatt gcggcgcaac gccttctgct cggccatcac
     1921 catcacttta tcgaagaaag cgtccaccgg cgccttcagc accgcgaagg tcttgaggta
     1981 accggcgtag tcgccgcgct cgaaaagctg gttggcgctg cccgaggcct tttgcagcgc
     2041 gtcgaagagt gcgatctccg ccgcctcttt cagcgccgag cgctcggcgt tggcaaagga
     2101 ttcgcctttc tccgcggcct gcctgaggat gttagccacc cgcttgttgg cggccgccag
     2161 gctttcggct tccggcagcg cctggaacgc cttcaccgcc tcgagctgct gcggtacgac
     2221 gccgagctgg accggccgct gggagaggac ggagtccacc tgcaacgtcg aaaacccctg
     2281 ctccttgagg taaccctcga agcgggcgaa cagaaattcg atcaggtcgg tgctggcctt
     2341 tgcccggtgg ccttcgaaag ccgccgaagc gagctcgttc agaggcaggg ggagcttgcg
     2401 ctcgacgatg atcctgatca cccccagcgc agcgcggcgc aggccgaacg gatccttctc
     2461 gccggtgggc tgctcgccga tgctgaacag gccggccagc gtgtcgagct tgtcggcgag
     2521 cgccacggcg caggcaatgc tgccttcggg cagccggtcg ccggcgaaac gcggccggta
     2581 gtgcgactcg atggcgtcgg cgactgcggc cggctcgccg tcgtgcagcg cgtagtagcg
     2641 ccccatgatg ccctgcagct ccggaaattc gccgaccatg ccggtcagca gatcggcctt
     2701 gcacagctcc gcggcgcgct ccgcgatcag cggatcggcc ttgagggcgc gcgcgatccc
     2761 gcccgccagg agcttgatgc gctgcacccg ctcgagctgg ctgccgagct tgctgtggta
     2821 gacgaccttg gcgagctgcg gcacgcgcgt ctcgagccgc gccttgcgat cctggttgta
     2881 gaagaagcgc gcatcctcca gccgcgggcg caccacgcgc tcgttgccgc cgatgatgtg
     2941 gcgcggctcg gccaccttca tgttcgagac gatcaggaac ctcggcagca gcttgccgcc
     3001 ggcgccgaac agcgggaagt acttctggtt ctgccgcatg gtgaggatca ggcattcctg
     3061 gggcacttcc atgaacgcct cttcgaacgc gccggcatag atggccggga actcgaccag
     3121 cgcggcaacc tcgtcgagca gcgcctgata ttcgcc
//
LOCUS       DASYJA010000187         5346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1390969, whole genome shotgun sequence.
ACCESSION   DASYJA010000187 DASYJA010000000
VERSION     DASYJA010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390969"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..419)
                     /gene="fdxA"
                     /locus_tag="VGO02_04050"
     CDS             complement(81..419)
                     /gene="fdxA"
                     /locus_tag="VGO02_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008528408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEV7476893.1"
                     /translation="MTYVVTEDCIKCKHMDCVEVCPVDCFYEGDNMLVIHPDECIDCG
                     VCEPECPPKAILPDSDGKADKWLKLNAEYAVKWPNIARKGTPPPDADAWKEVPDKFEK
                     FFSPEPGKGE"
     gene            549..2018
                     /locus_tag="VGO02_04055"
     CDS             549..2018
                     /locus_tag="VGO02_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEV7476894.1"
                     /translation="MSFAPLMAPLAVLAAVLVGGAALALYGVMRRTRAAEQALRHTER
                     QLRLVAESDARRFEFLAHHDPLTGLPNRAMFEERAREAVARARRHGETAALLFLDLDN
                     FKEINDSLGHEVGDALLRAAGERLRGCVRGEDFVARIGGDEFCVLLQGLGDPREAAGV
                     AQKIVKTLAQPCRIGEHEVACGANVGIACTPQDSAEAAELLRLADAAMYRAKDAGRHA
                     YRFAAPNINRELHASAALAEELRASLERDELFVCYQPRIDFLTRRPVAAEALLRWPHP
                     RFGLLPPESFLPLAEDSGIAAALGAMLLSKACAQAKRWRDAGAKDFSIAISVSTRTLR
                     HPGLPGEVRAALAASGLAPGALLLELPEAGLRQAAAPVREALAALADTGARLGVDDFG
                     AGYASLPLLRSLRIGAVSIDRRLVSSIPSDRDAPGLVRGLIALARGLDMDVVVRGIDT
                     AAHCNFVIAAGGLVGQGAFLAPTGLATEVEPLLATRRAA"
     gene            2116..4089
                     /locus_tag="VGO02_04060"
     CDS             2116..4089
                     /locus_tag="VGO02_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEV7476895.1"
                     /translation="MPRYRFVSAFAPLIIALGALLLFVAAATEIVPALRAWLSAGAPL
                     PRELMLHALIASAALLLVAAVLMMRRSGRRTELAEEALRESEARMRLVANSVPALISY
                     VDREQRYRFSNRTYDDWFGIAHESMHGRTVAEVFGDEAYAPMRGNFERVLTGEEVQFE
                     FTTAEGGRRRTLQVACVPHFDPQREKEAGAVLGFYMLASDVTALKRAQEDLRFAALQL
                     QHDARRLEFLAHHDTLTGLPNRAMFAERAREAVAHARRHEKTAALLFLDLDNFKQIND
                     SQGHEVGDNLLKQIAARLRAAVRGDDFVARIGGDEFCVLLQDIAEPREAAAVAQKLVH
                     ELGRPYSVAGAELVSGASIGIACVPHDGEEVDTLLRLADTAMYRAKQRGRNGYEFFSA
                     LLNQDAAEAAALADELRAGIGRGELFLVYQPRVDIATRRLVGAEALLRWRHPRRGVLT
                     PDAFLPLADESGLLVPIGAWVLREACAQARRWLDAGIRPCRVAINITARQLRHGALPE
                     QVRSALEASGLPGESLLIELPESALRQVPEPLRQALAAVRASGARLGVDDFGTGYASL
                     PMLQRLGIGAVCIDRRLVAGVPADGERAGLARALIALARGLDFEVVAKGVENHAQREF
                     LADAGCRVCQGELFAPAGTAAEVEPVLRARLAA"
     gene            complement(4096..4623)
                     /locus_tag="VGO02_04065"
     CDS             complement(4096..4623)
                     /locus_tag="VGO02_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HEV7476896.1"
                     /translation="MIPSRRRLLAAGLTLPFAADALAQSCARTLSQTEGPFFKTDTPL
                     RTSFLEKDARTRLVVSGQVLSAQCRPFANALLEFWHADEFGEYDNKGYRYRGHQHADA
                     QGRYRLETIVPAEYPGRARHIHVKVQAPGGRVLTTQLYFPDEPGNRRDGIYRPELEMK
                     IAKPGEGVYDFVVEA"
     gene            complement(4620..5093)
                     /locus_tag="VGO02_04070"
     CDS             complement(4620..5093)
                     /locus_tag="VGO02_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476897.1"
                     /translation="MTVLAAAGCNVVPHKAYDGPSRPERELSILRGGAFGDELNPVSL
                     VDLRVIDGKPQRAGRYLASVLPGRHTIGLSETLRIATRTRTQYCAVELDTLAGCLYIP
                     RPPSPPSDAQSGRKADWEWSVDMPITAECEAGGSFQTRVPARCGGSASILERPAR"
     gene            complement(5102..>5346)
                     /locus_tag="VGO02_04075"
     CDS             complement(5102..>5346)
                     /locus_tag="VGO02_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEV7476898.1"
                     /translation="AESFHSCAHGAGRRMSRNQARKVFSVEKLIEQTKGVVCRKDRGV
                     LDEIPGAYKDIDEVMANQSDLVEVVHTLKQVLCVKG"
BASE COUNT          748 a         1876 c         1865 g          857 t
ORIGIN      
        1 ccgcgagcgt ttctgcacgc gctgcggtgc ccgtcagcgt ctacccagtg taaacgcgtg
       61 gcgggagggt tttcttcccg ctattcgccc ttgccgggct cgggcgagaa gaatttctcg
      121 aatttgtccg gcacttcctt ccacgcgtcg gcatccggcg gcggcgtgcc cttgcgcgcg
      181 atgttcggcc atttgaccgc gtactcggcg ttgagcttga gccacttgtc ggccttgccg
      241 tccgaatcgg gcaggatggc cttcggcggg cactcgggct cgcacacccc gcagtcgatg
      301 cactcgtcgg gatggatgac gagcatgttg tcgccttcat agaagcagtc gacggggcag
      361 acctcgacgc agtccatgtg cttgcacttg atgcagtctt cggtgacgac gtaagtcatt
      421 cgatcgcggt ttgatagaaa gcgccattat aggcgtagta caaaaaggcg aacctgtaga
      481 tgtttcgcta cagcccgggc gtgaagtagt ccattgtcgg gcgcccgcag gcacccgaag
      541 attcatgcat gagcttcgct cctctgatgg caccccttgc ggtgcttgcc gccgtgcttg
      601 taggcggcgc agcgctcgcc ctgtacggcg tcatgcgccg cacccgggcc gccgagcagg
      661 ctctgcggca taccgagcgg cagcttcgcc tggtggccga gagcgacgcg cggcgcttcg
      721 aattcctggc acaccacgat ccccttaccg ggctgccgaa ccgcgccatg ttcgaggaac
      781 gggcgcgcga ggcggtggcc agggcgcggc gccatggcga aacggccgcc ctgctatttc
      841 tcgacctcga caatttcaag gagatcaacg acagcctcgg tcatgaggtg ggcgacgcgc
      901 tgctgcgcgc cgcgggcgag cggctgcgcg gctgcgtacg cggcgaggac ttcgtcgccc
      961 gcatcggagg cgacgagttc tgcgtgctgc tgcagggcct cggcgacccg cgcgaagctg
     1021 caggcgtagc acagaaaata gtcaaaacgc tcgcccaacc ctgccgcatc ggcgaacacg
     1081 aggtcgcttg cggtgccaac gtcggcattg cgtgcacgcc gcaggacagc gccgaggcgg
     1141 ccgagctgct gaggctggcc gatgccgcca tgtatcgcgc caaggacgcc ggccgccacg
     1201 cctaccgctt tgcggcgccc aacatcaacc gcgagctgca cgcctcggcc gcgctcgcgg
     1261 aggagcttcg cgcgagcctc gagcgcgacg aattgttcgt ctgctaccag ccgcgcatcg
     1321 atttcctcac ccgccggccg gtggcggccg aggcgctgct gcgctggccg catccgcgct
     1381 tcgggctgct gccgccggag tcgttcctgc cgctcgccga ggactccggc atcgccgccg
     1441 ccctgggcgc gatgctgctg agcaaggctt gcgcgcaggc gaagcgctgg cgcgacgcgg
     1501 gcgcaaagga tttctccatc gcgatttcgg tctcgacccg cacgctgcgc cacccaggtc
     1561 tgccgggcga agtgcgcgcg gcgctcgccg cgagcgggct ggcgcccggc gccctgctgc
     1621 tcgagttgcc ggaagccggg ctgcggcagg ccgccgcgcc ggtgcgcgag gcgctcgcgg
     1681 cgctggccga caccggcgcg cgcctcggcg tcgacgattt cggcgcgggc tacgcttcgc
     1741 tgccgctgtt gcgcagcctg cgcataggcg cggtgagcat cgaccggcgc ctcgtctcga
     1801 gtatcccgtc cgaccgcgac gcccccgggc tggtgcgcgg cctgattgcg ctcgcgcgcg
     1861 gcctcgacat ggacgtggtg gtgaggggta tagacaccgc ggcgcactgc aacttcgtga
     1921 ttgctgctgg cggcctcgtc ggccagggcg cttttcttgc cccgacgggg ctggccaccg
     1981 aggtggaacc gctgctggcg acgcggcgcg ccgcgtagta caaagagcca aaattgtaga
     2041 cgtaccgcta cagtcatagg cggattcgtc cattgcccca cccgccgcgc gggctcaata
     2101 tagctgcttc gtccgatgcc ccgttaccgc ttcgtttccg ccttcgcgcc gctgatcatc
     2161 gcgctcggcg cgctgctgct gttcgtcgcc gccgcgaccg agatcgtgcc cgcgctgcgc
     2221 gcctggctga gcgcgggcgc gccgctgccg cgcgagctga tgctgcacgc gctcattgcc
     2281 agcgcggcgc tgctgctggt cgccgcggtg ctgatgatgc gccgctcggg ccggcgcacc
     2341 gagctcgccg aggaggcgct gcgcgagagc gaggcgcgca tgcgcctggt ggcgaacagc
     2401 gtcccggcgc tcatctccta tgtcgaccgc gagcagcgct accgcttcag caaccgcacc
     2461 tacgacgact ggttcggcat cgcgcacgag agcatgcacg ggcgcaccgt ggccgaggtc
     2521 ttcggcgacg aggcctatgc gccgatgcgc ggcaatttcg agcgcgtcct gaccggcgag
     2581 gaagtgcagt tcgagttcac caccgcggag ggcgggcggc ggcgcaccct gcaggtggcc
     2641 tgcgtgcctc acttcgaccc gcagagggag aaggaggcgg gcgccgtcct cggcttctac
     2701 atgctggcga gcgacgtgac ggcgctcaag cgcgcccagg aggacctgcg cttcgccgcg
     2761 ctgcagctgc agcacgacgc gcggcgcctc gagttcctcg cgcaccacga caccctcacc
     2821 gggctgccca accgcgccat gttcgccgag cgcgcgcgcg aagccgtggc gcatgcccgc
     2881 cgccatgaga agaccgcggc gctgctgttc ctcgacctgg acaatttcaa gcagatcaac
     2941 gactcgcagg ggcatgaggt gggcgacaac ctgctcaagc agatcgccgc gcgcctgcgc
     3001 gccgcggtga ggggcgatga cttcgtcgcg cgcatcggcg gcgacgaatt ctgcgtgctg
     3061 ctgcaggata tcgccgagcc gcgcgaggcg gcggcggtgg cgcagaagct ggtgcacgag
     3121 ctgggcaggc cctacagcgt cgccggtgcg gagctggtct cgggcgcgag catcggcata
     3181 gcctgcgtgc cgcacgacgg cgaggaagtg gacacgctgc tgcggctggc cgacacggcc
     3241 atgtaccgag cgaagcagcg cgggcgcaac ggctacgagt tcttctcggc gctgctcaac
     3301 caggatgccg ccgaagcggc ggcgctcgcc gacgagctgc gcgccggcat cggccgcggc
     3361 gagctgttcc tcgtctacca gccgcgcgtc gacatcgcca cgcgccgcct ggtcggcgcc
     3421 gaggcgctgc tgcgctggcg ccacccgcgg cgcggcgtgc tgacgcccga cgccttcctg
     3481 ccgctggccg acgaaagcgg cctgctggtg ccgatcggcg cctgggtgct gcgcgaggcc
     3541 tgcgcgcagg ccaggcgctg gctcgatgcc ggcatccggc cgtgccgcgt cgccatcaac
     3601 atcacggccc gccagctgcg ccacggcgcg ctgccggagc aggttcgcag cgcgctcgag
     3661 gcgagcgggc tgcccggcga atcgctgctg atcgagttgc ccgagagcgc gctgcgccag
     3721 gtgcccgagc cgctgcgcca ggcgctcgcc gccgtgcgcg cgagcggcgc gcgcctcggc
     3781 gtcgacgact tcggcaccgg ctatgcctcg cttcccatgc tgcagcgcct gggcatcggc
     3841 gcggtgtgca tcgaccgacg cctggtcgcc ggcgtgccgg ccgacggcga gcgcgccggc
     3901 cttgcgcgcg cgctcattgc cctcgcccgc ggcctggatt tcgaggtggt ggcgaaaggc
     3961 gtggaaaacc acgcgcagcg cgaattcctc gccgacgccg gttgccgcgt gtgccagggc
     4021 gagctcttcg cccccgccgg caccgcggcc gaagtcgagc ccgtcctgcg cgcccgcctc
     4081 gccgcctagc ggccgctagg cctcgaccac gaagtcgtag acgccctcgc ccggcttcgc
     4141 gatcttcatc tccagctcgg ggcggtaaat gccgtcgcgc cggttgccgg gctcgtccgg
     4201 aaagtagagc tgggtggtca gcacgcggcc gccgggggcc tgcaccttga cgtggatgtg
     4261 gcgcgcgcgt cccgggtact cggcggggac aatcgtctcg aggcggtacc tgccctgcgc
     4321 atcggcgtgc tgatgccctc gataacggta gcctttgttg tcgtattcgc cgaactcgtc
     4381 ggcgtgccag aactcgagca gcgcattggc gaacggccgg cattgcgccg acaggacctg
     4441 gccgctcacc accagccgcg tgcgcgcatc cttctccaga aacgaggtgc gtagcggcgt
     4501 gtcggtcttg aagaaaggcc cctccgtctg gctgagggtc cgcgcgcagg actgcgccag
     4561 ggcatccgcg gcgaagggca gcgtcaggcc ggcggcgagg agtcggcggc gggaagggat
     4621 catcgtgctg gcctttcgag aatgctcgcc gagccgccgc agcgcgccgg cacccgggtc
     4681 tggaagctgc cgccggcttc gcattcggcc gtgatcggca tgtccacgct ccattcccag
     4741 tccgccttcc ttccggactg ggcgtcggac ggcgggctcg gcggcctcgg gatatagaga
     4801 cagcccgcca gggtgtcgag ttcaacagcg cagtactggg tgcgggtcct ggtggcgatg
     4861 cgcagcgtct cgctcaggcc gatggtgtgt cgcccgggca gcacgctcgc caggtaccgc
     4921 cccgcgcgct gcggcttgcc gtcgatgacg cgcaggtcga ccagcgacac cgggttcagc
     4981 tcatcgccga aagccccgcc gcgcaggatc gaaagctccc gctcgggccg cgacggcccg
     5041 tcgtaggcct tgtgcgggac gacgttgcag cccgccgccg cgagcaccgt caaaagcgcg
     5101 actagccctt gacgcacagc acctgcttca gcgtgtgcac gacctcgacg aggtcggact
     5161 ggtttgccat cacctcgtcg atgtccttgt aagcgccggg aatctcgtcg aggacgccgc
     5221 ggtctttgcg gcacaccacg ccttttgtct gttcaatcag tttttcaact gaaaagacct
     5281 tcctggcctg attgcggctc atcctgcgac ccgcgccgtg cgcgcaggaa tggaagcttt
     5341 ccgcgg
//
LOCUS       DASYJA010000188         3211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_120438, whole genome shotgun sequence.
ACCESSION   DASYJA010000188 DASYJA010000000
VERSION     DASYJA010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120438"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VGO02_04080"
     CDS             complement(<1..744)
                     /locus_tag="VGO02_04080"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEV7476899.1"
                     /translation="MNPTILDLIGNTPLVAVRRLDTGPCRLFLKLENQNPGGSIKDRS
                     ALAMIEAAERDGRLKPGALIVEATAGNTGLGLALVAAQKGYRLLIVVPDKMSQEKIFH
                     LRAMGAEVVLTRSDIGKGHPEYYQDRAARLAGEHPGSFYVNQFANHANPLAHERTTGP
                     ELWQQMAQDIDAVVCGVGTGGTMTGLSRFFATVSPQTEMVLADPEGSILAEFVRTGKI
                     PATVGSWLVEGIGEDFIPPIADLSRVRKAY"
     gene            824..2239
                     /locus_tag="VGO02_04085"
     CDS             824..2239
                     /locus_tag="VGO02_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476900.1"
                     /translation="MEAAVAAPGSGTLLGSISDFCRRTGMAESTFGRRAVNDGKFVAR
                     LRDGARVTPETLQRVNVFIQKHGLQGPGDSPPELLPLIRGGSPAPMPAPRADASPEKN
                     FRFFDNRQKYLLFVNTCSEKDVVARRVGLELGHIHPQPPAVRLFDAGMGDGTVLARVM
                     REMHRRFSTVPFYIVGKEISLEDVRLSLRKMADRFFEHPATVLVVTNMYYTEAPWLTP
                     RQVAAATSMVWHEAALTGNTSHDFSEQITALEPFLAKNWQAKANPKTGNPMYEQPVAL
                     VLYRDDYRFLLNDVIPKRGHARADYDMVIASQPYRLRAPLEFKAQKVVAPLTRALGKG
                     GRLLGIHSHGQDPGLEIIQKVWPGENPYTQSRHDLLRAVKAELGKDARRYNFNTYADA
                     RSLFRYDMHTLPSEISSAIGTSTLFAAWNAAIYVAQIDDQRLGEVLGRTQYLDATREV
                     LQKHGSLWFLDESYVVSRKRD"
     gene            2214..3092
                     /locus_tag="VGO02_04090"
     CDS             2214..3092
                     /locus_tag="VGO02_04090"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEV7476901.1"
                     /translation="MSSLASAIESGLQAPLAELVAASSLELAPRELHRAAEMAALLPA
                     DTCVYIPSLPGLPLSRTLEVVGAVRAAGLDPVPHVSARRVLERDEFRIFLKQCVQQHG
                     VHRVLVIGGDEPRPKGPYADSLQLIASGLLAECGVREIGVSGYPEGHPRISPVALQQS
                     FVRKVHLAKDQGLGLYVVTQFSFSPARMVEFCSNLARTAPQVGVYVGVAGPTDPAALA
                     RYAQRCGVIASLSALRTLGTGIARLVTHTDPREHVLALARYARSREPSNLVGLHFYSF
                     GGAVRTARWMSDLIAG"
BASE COUNT          505 a         1114 c         1053 g          539 t
ORIGIN      
        1 gtaggccttt ctgacccggg acaggtcggc gatcggcgga atgaagtcct cgccgattcc
       61 ctccaccagc cacgatccca cggtggcggg tatttttcct gtgcgaacga attccgcgag
      121 gatcgaccct tccggatccg ccagaaccat ttccgtttgc ggcgacaccg tggcgaagaa
      181 gcgcgacagg ccggtcatcg taccgccggt gccgaccccg cagaccacgg cgtcgatgtc
      241 ttgcgccatc tgctgccaga gctccggccc ggtggtgcgc tcgtgggcga gcgggttggc
      301 atggttcgcg aactgattga cgtagaacga gccgggatgc tccccggcca ggcgcgcggc
      361 ccggtcctgg tagtactcgg gatggccttt gccgatgtcg gagcgggtga ggaccacctc
      421 ggcgcccatc gcgcggaggt ggaagatctt ttcctggctc atcttgtcgg gaaccacgat
      481 cagcaggcgg tagcctttct gcgcggcgac cagggccagg cccagcccgg tgttgccggc
      541 agtggcctcg acgatcagcg caccgggctt cagccggccg tcgcgctcgg cggcttcgat
      601 catcgccagc gcgctgcggt ccttgatgga gccgcccggg ttctggtttt ccagctttaa
      661 aaacaagcga caaggccccg tatcgaggcg ccgcaccgcc accaacgggg tattgccgat
      721 aagatcgagg attgtgggat tcatcccaat tattgcaccg cacaccttgt ggttgaagtc
      781 ccactaaggt ggcgaggatg attttgctct acgaggaggg gctatggaag cggccgttgc
      841 ggcacccggt agcggcaccc tgttgggcag tatctccgac ttctgccggc gcaccggcat
      901 ggccgaatcg accttcggcc gccgcgcggt gaacgacggc aagttcgtgg cgcggctgcg
      961 cgacggcgcg cgcgtcaccc ccgagacgct gcagcgggtc aacgtcttca tccagaagca
     1021 cggcctgcag gggccgggcg actcgccgcc cgagctgctg ccgctgatcc gcggcggcag
     1081 cccggcgccg atgccggcgc cgcgcgccga cgcctcgccc gagaagaact tccgcttctt
     1141 cgacaaccgc cagaagtacc tgctgttcgt caatacctgc agcgagaagg acgtggtggc
     1201 gcgccgcgtc ggcctcgagc tcggccacat ccatccgcag ccgccggcgg tgcgcctgtt
     1261 cgatgcgggc atgggcgacg gcacggtgct cgcgcgcgtg atgcgcgaga tgcaccggcg
     1321 cttctctacc gtgccgttct atatcgtcgg caaggagatc agcctcgagg acgtgcgcct
     1381 gagcttgcgc aagatggccg accgcttctt cgagcacccg gcgacggtgc tggtcgtcac
     1441 caacatgtac tacaccgagg cgccgtggct cacgccgcgc caggtcgccg cggcgacctc
     1501 catggtgtgg cacgaggcgg cgctcaccgg caacacctcg catgacttca gcgagcagat
     1561 caccgcgctc gagccgttcc tcgccaagaa ctggcaggcg aaggcgaacc cgaagaccgg
     1621 caaccccatg tacgagcagc cggtggcgct ggtcctgtat cgcgacgact accgcttcct
     1681 gctgaacgac gtcattccca agcgcggtca cgcccgcgcc gactacgaca tggtgatcgc
     1741 ctctcaacct tacaggttga gagcgccgct cgagttcaag gcgcagaagg tggtagcgcc
     1801 gctgacgcgc gcgctcggca agggcggacg cctgctcggt atccactcgc acggccagga
     1861 cccgggcctg gagatcatcc agaaagtttg gccgggcgag aacccctaca cgcagagccg
     1921 ccacgacttg ctgcgcgcgg tgaaggccga gctcggcaag gacgcgcggc gctacaactt
     1981 caacacctac gccgacgcgc gctcgctgtt ccgctacgac atgcacacgc tgccgagcga
     2041 gatctcgtcg gcgatcggca cctcgacgct gttcgccgcc tggaacgcgg caatctacgt
     2101 cgcgcagatc gacgaccagc gcctgggcga ggtgctcggc aggacgcaat acctggacgc
     2161 gacgcgcgaa gtgctgcaga agcacggcag cctgtggttc ctcgacgaat cgtatgtcgt
     2221 ctctcgcaag cgcgattgag tccgggctgc aggcgccgct tgccgagctg gtggcggcga
     2281 gctcgctcga gctcgcgccg cgcgagctgc accgcgccgc agagatggcc gcgctgctgc
     2341 ccgccgacac ctgcgtctac atcccctcgc tgcctggctt gccgctgtcc cgcaccctcg
     2401 aggtcgtcgg cgcggtgcgc gcggcgggcc tcgacccggt gccgcacgtc tccgcgcgcc
     2461 gcgtcctcga gcgcgacgag ttccggattt tcctcaagca atgcgtccag cagcacggcg
     2521 ttcaccgcgt gctggtcatc ggcggcgacg agccgcggcc gaagggcccg tatgccgaca
     2581 gcctgcagtt gatcgcttcg ggcctgctgg ccgagtgcgg ggtgcgcgag atcggcgtat
     2641 ccggctaccc ggaagggcat cccaggattt cgcccgtggc cctgcagcag tcgttcgtgc
     2701 gcaaggtgca tctcgccaag gaccaggggc tcggccttta cgtggtgacc cagttctcgt
     2761 tctcgccggc gcgcatggtg gagttctgct ccaacctcgc gcgcaccgct ccgcaggtcg
     2821 gcgtctacgt cggtgtcgcc gggcccaccg accccgcggc gctcgcacgc tacgcgcagc
     2881 gctgcggcgt catcgcctcg ctcagcgccc tgcgcaccct cggcaccggc atcgcaaggc
     2941 tggtcacgca taccgacccg cgcgagcacg tccttgcact cgcccgctat gcgcgctcgc
     3001 gcgagccgag caacctcgtc gggctccact tctacagctt cggcggcgcg gtgcgcaccg
     3061 cgcgctggat gagcgacctc atcgccggct agaaatccgg gtcagagcac gatcttcagt
     3121 agtactcttg ctggtggctg cacgcggtgc cgcaatcgat ttcttcgtta ttacctgcga
     3181 gcacgggggc aatgggattc cggcgcctta t
//
LOCUS       DASYJA010000189         4261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1936794, whole genome shotgun sequence.
ACCESSION   DASYJA010000189 DASYJA010000000
VERSION     DASYJA010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1936794"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VGO02_04095"
     CDS             complement(<1..256)
                     /locus_tag="VGO02_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEV7476902.1"
                     /translation="MHLADAVNIEDLRLMAKRRVPRAIFDFFDGGAEDETTLRGNRAA
                     FERVRLLPKVLVDVSKIDTTVSLFGSEAKMPLAIAPTGGIS"
     gene            292..1251
                     /locus_tag="VGO02_04100"
     CDS             292..1251
                     /locus_tag="VGO02_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7476903.1"
                     /translation="MSRLFALAAALLVWLPAAAQTVPSKSLRIVVPFTPAGSVDIAAR
                     AIAHEMSKILGQSVVVENKPGAGGNLGALDVARSAPDGYSIVISTSGIQAINPALYAK
                     MPLDPNKDLASVVPLVSLSNVLVVHPSVPARSVKEIIALAKKDPGKWVYASSGSGTSI
                     HMSGAMFTQLTGTDILHIPYKGSAPAITDLLAGQTNMMFDNIPSALPHIKAGKLIALA
                     TTGAKRDPALPDLPTVAEAGVPGYEAGVWMGLMVPAATPKDIIARLNAAAVQGAKSPE
                     YVKRMTDLGYNIIAGSSEEMARMLEVEIRRWTPIVKASGAKVD"
     gene            1251..2186
                     /locus_tag="VGO02_04105"
     CDS             1251..2186
                     /locus_tag="VGO02_04105"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV7476904.1"
                     /translation="MNKPALSKVKRIEGVLSPVVTPFRKDYSPDAERFVRHCKWLLKS
                     GCAGLAVFGTNSEANSLSVSEKRSLLEKLVSGGVPAASLMPGTGHCALSDSIEMTRAA
                     VELGCAGVLMLPPFYYKGVPDEGLFRNFAEVIERVGDERLQLYLYHIPPVSQVSISLN
                     LIERLLKKYPGSIAGVKDSSGDWSNTKAMLDAFAKNGFDVFAGSEVFLLDNMRHGGKG
                     CITATGNINPGPIDQVYRNWQTADADKLQAGITATRKIVQKQPMIPALKAAIAHFGKD
                     AEWKTCRPPLIELSSSEETTLISELRANGFSMPGL"
     gene            2183..2863
                     /locus_tag="VGO02_04110"
     CDS             2183..2863
                     /locus_tag="VGO02_04110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7476905.1"
                     /translation="MKTALVTGAARGIGHAASEALQKSGVKVLRLDREFPSRNPEQIF
                     YDLTDLAGIPKLVESLGQIDILVNNAGVQTAVGIDKYTDEQRTRILRINLEAPVELIR
                     AVSKQMVARRSGRIVNLASVAAYTAHTDLWYGVTKAGVVSFTRSFAALLGPQGIQVNA
                     VAPGPIDTPLLDKAQPERVDQLMKIVYTRRKGRPDEVAEAVRWLAVDAPVIINGAVVD
                     VTDGCVLR"
     gene            complement(2871..3833)
                     /locus_tag="VGO02_04115"
     CDS             complement(2871..3833)
                     /locus_tag="VGO02_04115"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEV7476906.1"
                     /translation="MRFLCFAFLALVMNAAGAQGYPNRPVTIVVPFSAGGPTDTIARF
                     LGERMSRTLGQTVLVENTAGAAGSIAMARVARAAPDGYLIGIGHIGPNVFNGAIYQLQ
                     YDLLKDFEPVAMVATNPQILVGKNALQAKDLKELVAWAKANGDKVSFGTAGAGSPSHV
                     TGVYFNQLTGSTAKAIHYRGAAPAMTDLISGQIDIYFDQAVTAVPNSRAGRVKAYAVT
                     ANKRIAAAPDIPTVDEAGLPGLYMSIWHAIWAPKGTPAAVVGRLNGAIVEALADEAVR
                     KRLMDLGQEIPSREQQTPEALGAHHKAEIEKWWPLIKAAGIKVD"
     gene            complement(3845..>4261)
                     /locus_tag="VGO02_04120"
     CDS             complement(3845..>4261)
                     /locus_tag="VGO02_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEV7476907.1"
                     /translation="IAGATVLDLSTSLRFHYSHVPGAWWGVRSRLSQAFEKIPSVKRL
                     ILTSEDGTLAHLAAPEAAKQFSRAEIAVLEGGNAAWAGPTESGIERVTTERDDVWYKP
                     YDHASDYKKHARDYLSWEVALLDQIKRDPTIRFRSY"
BASE COUNT          718 a         1433 c         1386 g          724 t
ORIGIN      
        1 cgctgatgcc gccggtgggg gcgatggcga gaggcatttt tgcttcactg ccaaaaagac
       61 ttaccgtggt gtcaattttc gagacgtcca ccagaacctt cggcagcagc cgcacccgct
      121 cgaacgctgc gcggttgccg cgcagggtgg tctcgtcctc ggcgccgccg tcgaagaagt
      181 cgaagatggc gcgcggcacg cggcgtttcg ccatcaggcg caggtcctcg atattgacgg
      241 cgtccgccaa gtgcataatt gcgggtcact ctaccttcga aagcccagcc cgtgtcgcgc
      301 ctgttcgcac tcgccgccgc cctgctggtc tggttgcctg ccgcagccca gaccgtcccg
      361 tccaagtccc tgcgcatcgt cgtgcccttc acccccgccg gctcggtcga catcgccgcg
      421 cgcgccatcg cgcacgagat gagcaagatc ctcggccagt cggtggtggt cgagaacaag
      481 cccggcgccg gcggcaacct cggcgcgctc gacgtggcgc gctcggcgcc cgacggctac
      541 tcgatcgtga tcagcaccag cggcatccag gcgatcaacc cggcgctcta cgccaagatg
      601 ccgctcgatc cgaacaagga cctcgcgtcg gtggtgccgc tcgtctcgct ctccaacgtg
      661 ctggtcgtgc acccctcggt tccggcgcgc agcgtcaagg agatcatcgc gctggccaag
      721 aaggacccgg gcaagtgggt ctacgcctcg agcggcagcg gcaccagcat ccacatgtcg
      781 ggcgcgatgt tcacgcagct caccggcacc gacatcctgc acatcccgta caagggcagc
      841 gcgccggcga tcacggacct gctggccggg cagaccaaca tgatgttcga caacatcccg
      901 tcggcgctgc cgcacatcaa ggcgggcaag ctgatcgcgc tcgccaccac cggcgcgaag
      961 cgcgatccgg cgctgcccga tctgcccact gtcgccgagg ccggcgtgcc cggctacgaa
     1021 gccggcgtgt ggatggggct catggtgccc gcggcgacgc cgaaggacat catcgccaga
     1081 ctgaacgcgg ccgcggtgca gggcgcgaag tcgcccgagt acgtcaagcg catgacggat
     1141 ctcggctata acatcatcgc cggctcgtcc gaggagatgg cgcgcatgct ggaagtcgag
     1201 atcaggcgct ggacgccgat cgtgaaggct tcgggcgcaa aggtggactg atgaacaagc
     1261 ccgcactcag caaggtgaag cgcatcgagg gcgtgctctc gcccgtggtc acgccgttcc
     1321 gcaaggacta ttcgcccgac gccgagcgct tcgtgcgcca ctgcaagtgg ctgctgaaga
     1381 gcggctgcgc cgggctggcg gtgttcggca ccaacagcga ggccaattcg ctttctgttt
     1441 ctgaaaaacg ttctttattg gaaaaactcg tgagcggcgg cgtcccggct gcgagcctga
     1501 tgcccggcac cgggcattgc gcgctgtccg actcgatcga gatgacgcgc gccgcggtcg
     1561 agctcggctg cgccggcgtg ctgatgctgc cgcccttcta ctacaagggc gtgcccgacg
     1621 aggggttgtt ccggaatttc gccgaggtga tcgagcgcgt cggcgacgag cggctgcagc
     1681 tgtacctgta tcacatcccg ccggtctctc aagtctccat ttccttgaat ctgatagaga
     1741 gactgctcaa gaaatatccg ggcagcatcg ccggggtaaa ggactcgtcg ggcgactggt
     1801 cgaacaccaa ggcgatgctc gacgcgttcg cgaagaacgg cttcgacgtc tttgccggca
     1861 gcgaggtgtt cctgctcgac aacatgcgcc acggcggcaa gggctgcatc accgccaccg
     1921 gcaacatcaa ccccggcccg atcgaccagg tctaccgcaa ctggcagacc gcggacgccg
     1981 acaagctcca ggccggcatc accgccacgc gcaagatcgt gcagaaacag ccgatgatcc
     2041 ccgcgctcaa ggctgcgatc gcgcacttcg gcaaggatgc ggagtggaag acttgccggc
     2101 cgccgctgat cgaactttct tcttctgaag aaacgactct aatttcagaa ctgcgagcca
     2161 acggcttcag catgcccggc ctgtgaaaac cgcgctggtc accggcgccg cgcgcggcat
     2221 cggacatgcg gccagcgaag cgctgcagaa atcgggcgtg aaggtcctgc ggctggacag
     2281 ggaatttccc agcaggaatc ctgagcagat cttctacgat ctgacggatc tggcgggcat
     2341 cccgaagctg gtcgaatcgc tcggccagat cgacatcctg gtcaacaacg cgggcgtgca
     2401 gacggcggtc ggcatcgaca agtacaccga cgagcagcgc acgcgcattc ttcgcatcaa
     2461 cctcgaggcg ccggtggagc tgatccgggc ggtgtcgaag cagatggtgg cgcgcaggag
     2521 cgggcgcatc gtcaacctcg cctcggtggc ggcctacacg gcgcataccg acctctggta
     2581 cggcgttacc aaggccggcg tcgtgagctt tacccgcagc ttcgccgcgc tcctcgggcc
     2641 gcaggggatc caggtgaacg cggtggcgcc cggcccgatc gacacgccgc tgctcgacaa
     2701 ggcgcagccc gagcgcgtcg accagctgat gaagatcgtc tatacgcgcc gcaagggccg
     2761 acccgacgag gtcgccgaag ccgtgcgctg gcttgccgtc gacgcgccgg tgatcatcaa
     2821 cggcgcggtg gtcgacgtca ccgacggctg cgtgctgcgc taggcgcgcg ctagtcgacc
     2881 tttatcccgg ccgccttgat gagcggccac catttctcga tctcggcctt gtgatgcgcg
     2941 cccagcgcct ccggtgtctg ctgctcgcgg gacgggatct cctggccgag gtccatcagc
     3001 cgcttgcgca ccgcctcgtc ggcgagcgct tccacgatcg ccccgttcag gcggccgacc
     3061 actgcggccg gcgtgccctt cggcgcccag atggcgtgcc agatgctcat gtagagaccg
     3121 ggcaggccgg cctcgtccac ggtcgggatg tccggcgccg ccgcgatccg cttgttcgcg
     3181 gtcacggcgt acgccttcac ccggccggcg cgcgagttcg gcaccgcggt gaccgcctgg
     3241 tcgaagtaga tgtcgatctg cccggagatc aggtcggtca tcgcgggcgc cgcgccgcga
     3301 tagtggatcg ccttcgccgt gctgccggtg agctggttga agtagacgcc ggtgacgtgc
     3361 gaggggctgc cggcgccggc ggtgccgaac gacaccttgt cgccgttcgc tttcgcccac
     3421 gccaccagct ccttcaggtc tttcgcctgc aaggcattct tgccgaccag gatctgcggg
     3481 ttggtggcga ccatcgccac cggctcgaaa tccttcagca ggtcgtactg cagctggtag
     3541 atcgcgccgt tgaagacgtt cggtccgatg tggccgatgc cgatcaggta gccgtcgggc
     3601 gccgcgcgcg ccacgcgggc catcgcgatg ctgccggccg cgccggccgt gttctccacc
     3661 aggacggtct ggccgagcgt tctggacatg cgttcgccga ggaacctcgc gatcgtatcg
     3721 gtcggcccgc cggccgagaa cggcaccact atcgtgaccg gtctgttcgg ataaccctgt
     3781 gcgcccgccg cgttcatcac gagcgcgaga aacgcgaagc ataagaacct catggaccct
     3841 cctgttagta gctgcggaag cggatcgtcg gatcgcgctt gatctggtcc agcagcgcga
     3901 cttcccagct caggtagtcg cgcgcgtgct tcttgtagtc cgaggcgtgg tcgtaaggct
     3961 tgtaccagac gtcgtcgcgc tcggtggtca cgcgctctat ccccgactcg gtcggacccg
     4021 cccaggcggc gttgccgccc tcgagaactg cgatttcggc tctcgagaac tgttttgccg
     4081 cttcgggtgc ggcgaggtgg gccagcgtgc cgtcttcgga cgtcaagatc aggcgtttca
     4141 cagaaggtat tttttcaaaa gcctgtgaaa gccgggagcg cacgccccac cacgcccctg
     4201 gaacgtgcga atagtggaag cgcaggctgg tcgacaggtc cagcaccgtc gccccggcga
     4261 t
//
LOCUS       DASYJA010000190         3392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2027176, whole genome shotgun sequence.
ACCESSION   DASYJA010000190 DASYJA010000000
VERSION     DASYJA010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2027176"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /gene="mutM"
                     /locus_tag="VGO02_04125"
     CDS             complement(<1..791)
                     /gene="mutM"
                     /locus_tag="VGO02_04125"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEV7476908.1"
                     /translation="MPELPEVEVTRRGLAPQLAGRVISGVEVREPRLRWPIGADVRRL
                     AGRTVKAVRRRGKYLLVDCGDGHLILHLGMSGSLRLLPPGTPAGKHDHFDLLFGDRIL
                     RLRDPRRFGAVLWAPGDAKTHRLLAHLGVEPLSRALDAARLHALTRPHRTAIKLFLMD
                     ARRVVGVGNIYASESLFRAGINPRMKANRISLEKSRKLSAAIKLTLNKAIRAGGSTLR
                     DFVGADGNPGYFQQRYWVYDRAGQPCRRCGTTIRRIVQGQRSTFF"
     gene            849..2540
                     /locus_tag="VGO02_04130"
     CDS             849..2540
                     /locus_tag="VGO02_04130"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV7476909.1"
                     /translation="MPKPFYRLALAVALACAALPAAAQGKKSVPPAAQSSELSEPMLY
                     EFLLGEIALQRGDAGLAAQTYVDLAKRTRDPRIARRAVEVANQARMPELALEAAKLWQ
                     EIEPASAQALQVVAALLVTSKRVEEAEPYLVKLLAADGVNVENGFMQMNRLLAGNPDK
                     AANVRVVRNLAAKYPELAQAQFAVAQAAALAGDDAAALEAIRRAGKLRPDWETAAIFE
                     AQLLQKQSPAEAAKVLGNFVEKYPTAREARINYARVLVLDKRFPEARKQFEALLAASP
                     GNTEVIYAVGLLAFQLKDYAVAEENMKRLLGLKYRDPSGVRYVLGQIAEEQKHWPEAI
                     KWYESVADGDQVIPARVRAAGAMAKQGKVDEARALLQRVGDEFPDEKVQFTVAEAQLL
                     RDANRNREAYDMLGQALKQDPEQPELLYDYALTAEKLERYDVLETNLRKLIQVRPDHA
                     HAYNALGYSFAERNTRLPEAKKLIEKALEIAPEDYFIIDSLGWVLYREGDLKGAAEQL
                     RRAYGGRPDAEIGAHLGEVLWVMGQRAEADKVWQESLKASPENESLQKTIKRFKK"
     gene            2540..3019
                     /gene="lolB"
                     /locus_tag="VGO02_04135"
     CDS             2540..3019
                     /gene="lolB"
                     /locus_tag="VGO02_04135"
                     /inference="COORDINATES: protein motif:HMM:NF015512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HEV7476910.1"
                     /translation="MRWALLALVLLSACAGIERSSIDKPEFDLAGRIAARYRDESFSG
                     NLAWRHARGADEMLISTALGQGVARIVREGEAVTLTTAEPREYKAADAESLTEQVLGF
                     RVPLAGLAQWVQGLPAPELEARGWKVEYQERDAQGRPRRLRLTYPGVELRLAISEWK"
     gene            3016..>3392
                     /gene="ipk"
                     /locus_tag="VGO02_04140"
     CDS             3016..>3392
                     /gene="ipk"
                     /locus_tag="VGO02_04140"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="An essential enzyme in the nonmevalonate pathway of
                     isopentenyl diphosphate and dimethylallyl diphosphate
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEV7476911.1"
                     /translation="MKWYPAPGKLNLCLHVLGRRETGEHAGYHELQTVFRLVDVADRV
                     GISPRDDAEVRFSGAFGEDNLCLRAARLLRQESGSKKGCDLALDKILPVGGGMGGGSS
                     DAATVQLVLNRLWGLNLRKEILLR"
BASE COUNT          572 a         1133 c         1202 g          485 t
ORIGIN      
        1 cagaagaagg tcgagcgctg cccctgcacg atgcgccgga tggtcgtgcc gcagcgccgg
       61 cagggctgcc cggcgcggtc gtagacccag tagcgctgct ggaaatagcc cggattgccg
      121 tccgcgccga cgaagtcgcg cagggtcgag ccgccggcgc ggatcgcctt gttcaatgtg
      181 agtttgatgg cggccgacag cttcctgctt ttctcaagcg aaatccggtt cgctttcatg
      241 cgcggattga tgccggcgcg aaacaggctt tcgctcgcgt agatgttgcc gacgccgacg
      301 acgcggcgcg catccatgag gaaaagcttg atcgcggtgc gatgcgggcg ggtcagcgcg
      361 tgcagccgcg ccgcgtcgag ggcgcgcgac agcggctcca cgcccagatg cgcgagcagc
      421 cggtgcgtct tcgcatcgcc gggtgcccag agcaccgcgc cgaagcggcg cggatcgcgc
      481 aggcgcagga tgcggtcgcc gaagaggagg tcgaagtggt cgtgcttgcc cgccggcgtg
      541 cccggcggca gcaggcgcag gctgcccgac attcccagat gcaggatgag atggccgtcg
      601 ccgcagtcga ccagcaggta cttgccgcgg cggcgcaccg ccttgaccgt gcgcccggca
      661 agccgcctca cgtccgcgcc gatcggccag cgcaggcgcg gctcgcgcac ctcgacgccc
      721 gagatgacgc ggccggcgag ttgcggcgcg aggccgcggc gggtgacttc gacttccggc
      781 agctctggca tgtggaacgg ggcggcttgt gcggggcacc aaatatacct aagatgggtc
      841 gaacccagat gcccaaacct ttctaccgcc ttgcactcgc cgtggcgctg gcctgcgcgg
      901 cgcttcccgc cgccgcgcag ggaaagaaga gcgtgccgcc ggccgcgcag tcttccgagc
      961 tcagcgagcc gatgctgtac gaattcctgc tcggcgagat cgccctgcag cgcggcgatg
     1021 ccggcctcgc ggcgcagacc tacgtcgatc tcgccaagcg tacgcgcgac ccgcgcatcg
     1081 cgcgccgcgc ggtggaagtg gccaaccagg cgcgcatgcc cgagctcgcg ctcgaggccg
     1141 ccaagctctg gcaggagatc gagcctgctt ccgcgcaggc gctgcaggtg gtggcggcgc
     1201 tgctggtcac ctcgaagcgc gtcgaggagg ccgagcccta cctggtcaag ctgctcgccg
     1261 ccgacggcgt caacgtcgag aacggcttca tgcagatgaa ccggctgctc gccggcaacc
     1321 cggacaaggc ggcgaacgtg cgcgtggtgc gcaacctcgc ggcgaagtat ccggagctcg
     1381 cccaggcgca gttcgcggtg gcgcaggccg ccgcgctcgc cggcgacgac gcggccgcgc
     1441 tcgaggcgat ccgccgcgcc ggcaagctcc gtcccgattg ggaaacggcc gcgatcttcg
     1501 aggcgcagct gctgcagaag cagtctccgg ccgaagcggc gaaggtgctc ggcaacttcg
     1561 tcgagaagta tccgacagcg cgcgaggcgc gcatcaacta cgcgcgcgtc ctggtgctcg
     1621 acaagcgctt ccccgaagcg cgcaagcaat tcgaggcgct gctcgccgcc agcccgggca
     1681 acaccgaggt gatctacgca gtcgggctgc tcgccttcca gctcaaggac tacgccgtcg
     1741 ccgaggagaa catgaagcgg ctcctcggcc tgaaataccg cgacccgagc ggggtgcgct
     1801 acgtcctggg ccagatcgcc gaggagcaga agcactggcc cgaggcgatc aagtggtacg
     1861 agagcgtcgc ggacggcgac caggtcatcc cggcccgcgt gcgcgccgcc ggcgcgatgg
     1921 ccaagcaggg gaaggtggac gaggcgcgcg ccttgctgca gcgcgtggga gacgaattcc
     1981 ccgacgagaa ggtgcaattc acggtggcgg aggcgcagtt gctgcgcgat gcgaaccgca
     2041 accgcgaggc ctacgacatg ctcggccagg cgctcaagca ggatcccgag cagccggagc
     2101 tgctatacga ctacgcgctc accgccgaga agctagagcg ctacgacgtg ctcgagacca
     2161 atctgcgcaa gctgatccag gtgcggccgg accatgcgca cgcctacaac gcgctcggct
     2221 actcgttcgc cgagcgcaac acccgcctgc cggaggcgaa gaagctgatc gaaaaagcgc
     2281 tcgagatcgc gcccgaggac tacttcatca tcgacagcct gggctgggtg ctgtaccgcg
     2341 agggcgacct caagggcgcg gccgagcagc tgcgccgcgc ctacggcggg cggcccgacg
     2401 ccgagatcgg cgcccacctc ggcgaggtgc tctgggtgat ggggcagcgc gccgaggccg
     2461 acaaggtctg gcaggaatcg ctcaaggcca gcccggagaa cgagtccctg cagaagacaa
     2521 tcaagcgttt caagaagtaa tgcgctgggc gctgctcgcc ctggtactgc tgtctgcctg
     2581 cgcgggcatc gagcgctctt cgatcgacaa accagaattc gacctggccg gacgcatcgc
     2641 cgcgcgttat cgcgatgaat ccttcagcgg gaaccttgcc tggcgccatg cgcgcggtgc
     2701 cgacgagatg ctgatcagca ccgcgctcgg ccagggcgtg gcccgcatcg tgcgcgaggg
     2761 cgaggccgta acgctgacca ccgccgagcc gcgcgaatac aaggccgcgg atgctgaatc
     2821 gctcaccgag caggtgctcg gcttccgcgt gccgctcgcc ggactggccc aatgggtgca
     2881 ggggctgccg gctcccgagc tcgaggcccg aggctggaag gtcgagtatc aggagcgcga
     2941 cgcccagggc cggccgaggc ggttgcgcct gacctatccc ggcgtggagc tgcggctcgc
     3001 gatttccgag tggaaatgaa gtggtatccc gcgccgggca agctcaacct ctgcctgcac
     3061 gttctcggcc gccgcgagac gggggaacat gcgggctacc acgagctgca gaccgtcttt
     3121 cgcctggtcg acgtcgccga ccgcgtcggc atttcgccgc gcgacgatgc cgaggtgcgt
     3181 ttttccggag ccttcggcga ggacaacctg tgcctgcgtg cggcgcggct gctgcggcag
     3241 gaaagcgggt cgaagaaggg ctgcgatctg gcgctcgata agatcctgcc cgtcggtggc
     3301 gggatgggcg gcggctcctc cgacgccgcg accgtccagc tggttcttaa ccggttgtgg
     3361 ggattgaatt taaggaagga aatcctcttg cg
//
LOCUS       DASYJA010000191         4554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1029502, whole genome shotgun sequence.
ACCESSION   DASYJA010000191 DASYJA010000000
VERSION     DASYJA010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029502"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VGO02_04145"
     CDS             complement(<1..956)
                     /locus_tag="VGO02_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEV7476912.1"
                     /translation="MVDLTSVVRPGDGIIWGQACAEPQTLVEALVAQRAQLSGVGVFL
                     GSSYSGLVTPQHADHLRLSSYCGTARNRALADAGVLEILPAPYSQLGALIRSQKIRSD
                     VVMVQVSPPNAKGEYSLGLGVEYLAPALEKARAVVAEVNQRVPWTWTEPLLRKEDFAL
                     LVQSSRPPVYVDFKPSEADREIARHAERFVPEGAVLECGIGSLPNAVLAALKGRRGLA
                     YHSGLICEGVAELDLRSCVGGALMGSQRLFDWARENPRVQLRSSDYTHGAATLARIER
                     FVAINSAVEVDLTGQVNGELAQGSYVGAVGGALDFIRAANASP"
     gene            984..1550
                     /locus_tag="VGO02_04150"
     CDS             984..1550
                     /locus_tag="VGO02_04150"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007732115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HEV7476913.1"
                     /translation="MLDLILRKARSHGDFTDQPVPEELLRAAHELMKWGPTSANSQPM
                     RILYLRSKEAREKLRPSLSATNLEKTMKAPLVALVAYDTRFWENLPRMFHIPDAINWF
                     KDKGAHTEITAFRNGTLQGGYYLLALRAVGLDCGAMSGFDLGKADAAFFPDGRLKSNF
                     LVNIGYGTGKGIAPRNPRLSFEETCKIL"
     gene            complement(1547..2455)
                     /locus_tag="VGO02_04155"
     CDS             complement(1547..2455)
                     /locus_tag="VGO02_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEV7476914.1"
                     /translation="MPTSSTNLERYAWLSVAAALATMGLKTLAWWLTGSVGLLSDAIE
                     SLVNLAAALLALSMLRLAARAPDKDHPYGFSKAEYFSAGIEGALIVLAAAGIIATALP
                     RLASPQPLGMPGLGLAISVAATGINLAVAMVLLRAGKAHRSITLEADGKHLMTDVWTS
                     VGVVAGVGLVFATGWLVLDPLVALAVAVHIVWAGVGLMRRSVSGLLDAAIPQADRDAI
                     EKIFAEYSRRYGCAFHAVRTRRAAARRFVSFHLLVPDEWTVAHAHRLSEEIEERIREL
                     LDGATIDTHIEPISDPASYEDQKLDR"
     gene            complement(2430..2897)
                     /locus_tag="VGO02_04160"
     CDS             complement(2430..2897)
                     /locus_tag="VGO02_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476915.1"
                     /translation="MANRGLLLTITEPPPAMEEEFNAWYDDEHLPERLAIPGFRSARR
                     WTWQKSYLATYELDSPGVLNSPQYLSRYENPTPWTKRCLGRVVVFKRWACEQLGSEDP
                     HPGTKAMIVGFDERAFSPPGMLQARRFAASSGESITLYELAEAAAGAYELYKS"
     gene            complement(2887..3540)
                     /locus_tag="VGO02_04165"
     CDS             complement(2887..3540)
                     /locus_tag="VGO02_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7476916.1"
                     /translation="MALLEIAGLSGGYGRAQVLFDLRLEIGAGEVVALLGRNGAGKST
                     TLKTVMGLLPAFGGEVRLDGRRIDRLEPYEIARAGLGYVPEERRIFTDLTVEENLEVG
                     RRPASPGVAAWTQERLLALFPALAGMLGRRAALMSGGEQQMLCIARTLAGNPRVILLD
                     EPSEGLAPLVVAQIARAIAELKGAGVTVLLAEQGSNLTRRLADRSYTLDRGRIIDGQ"
     gene            complement(3527..4267)
                     /locus_tag="VGO02_04170"
     CDS             complement(3527..4267)
                     /locus_tag="VGO02_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7476917.1"
                     /translation="MAVLEVSNLAKAYDGVQALRGVSFAVGAGELVAMIGPNGAGKTT
                     CFNIVNGQLRPDAGEVSLGGTSLVGMPPRAVWRLGVGRTFQVAAAFGSMSVRENVQIA
                     LLSRDRLLGSFFLPAENLLVEEANELLGRVGMAQQAARPCGELAYGDLKRLELAIALA
                     NRPRLLLMDEPTAGTAPEERDALMGLASSLARSEKIAVLFTEHDMEAVFGFADRVLVL
                     HGGELIATGAPGEVRDDPRVREVYLGPA"
     gene            complement(4249..>4554)
                     /locus_tag="VGO02_04175"
     CDS             complement(4249..>4554)
                     /locus_tag="VGO02_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7476918.1"
                     /translation="FTIAGLFAGLAGALFAFSKGSISPETLSVPRSVDGLVMVLLGGL
                     QSLTGPVWGAALFTWLEDSVSRNVDYWRAALGAVILLLVLALPQGIVGLLKKWPSSR"
BASE COUNT          782 a         1562 c         1530 g          680 t
ORIGIN      
        1 ggcgatgcat tggcggcgcg gatgaagtcg agcgcgccgc cgaccgcgcc gacgtacgag
       61 ccctgggcga gctctccgtt gacctgtccc gtcaggtcga cctcgaccgc cgaattgatc
      121 gcgacgaagc gctcgatccg cgcgagggtc gccgcaccgt gcgtgtaatc ggaggagcgc
      181 agctgcacgc gcgggttctc gcgcgcccag tcgaacaggc gctgcgagcc catcagggcg
      241 ccgccgacgc aggagcgcag gtcgagctcg gccacccctt cgcagatcag tcccgagtgg
      301 taggcgaggc cgcgacggcc tttcagcgcc gcgagcacgg cgttgggcag cgacccaatg
      361 ccgcactcga gcaccgcgcc ctccggcacg aagcgctcgg catgccgggc gatctcgcgg
      421 tcggcctcgc tcggcttgaa atcgacgtag accggcggcc gcgagctttg cacgaggagc
      481 gcaaagtcct ccttgcgcag cagcggctcg gtccaggtcc agggcacccg ctggttgacc
      541 tcggcgacca cggcgcgcgc cttttcgagc gcaggtgcga ggtattcgac gccaagcccc
      601 aggctgtatt cgcctttggc gttgggtggg cttacctgca ccatgaccac gtcagacctt
      661 attttctggc ttcttatcag agcccctagc tgcgagtagg gcgccggcag gatctccaaa
      721 acgccggcat cagcgagggc gcggttgcgc gcggtgccgc agtaggacga caggcgcagg
      781 tggtcggcgt gctgcggggt gacgaggccc gagtagctcg agccgaggaa gactcccact
      841 cccgagagtt gggcacgttg ggcaacgagc gcctcgacca gggtctgcgg ctccgcgcaa
      901 gcctggcccc agatgattcc gtcgccggga cgaactacac tggttagatc gaccatcgag
      961 gacggattat gaccgacgag aaaatgctgg acctcattct gcgcaaagcg cgttcacacg
     1021 gcgacttcac ggatcagcct gtcccggagg agctgctgcg cgcagcgcac gagctgatga
     1081 aatggggccc gaccagcgcc aacagccagc cgatgcgcat cctctacctg cgctcgaagg
     1141 aggcgcgcga gaagctgcgc ccctcgctga gcgcgaccaa cctcgagaag acgatgaagg
     1201 cgccgctggt cgcgctggtc gcgtacgaca cgcgcttctg ggagaacctg ccgcgcatgt
     1261 tccacatccc cgacgccatc aactggttca aggacaaggg cgcccatacc gagatcacgg
     1321 cgtttcgcaa cggcacgctg cagggcggct actacctgct cgccttgcgc gcggtcggcc
     1381 tcgactgcgg cgccatgtcg ggcttcgacc tcggcaaggc ggacgccgct ttcttcccgg
     1441 acggacggct gaagtcgaat ttcctggtca acatcggcta tggcaccggc aagggcatcg
     1501 cgccccgcaa tccgcgcctg agcttcgaag agacctgcaa gatcctctag cgatccagct
     1561 tctggtcctc gtacgacgcg gggtccgaga tcggctcgat gtgcgtgtcg atggtcgcgc
     1621 cgtcgagcag ctcgcggatg cgttcctcga tctcttccga gaggcggtgc gcgtgcgcca
     1681 cggtccactc gtcggggacc agcaggtgga atgagacgaa gcgtcgcgcc gccgcgcgcc
     1741 gcgtgcgcac cgcatggaag gcacagccgt agcggcgcga atactcggcg aagatcttct
     1801 cgatcgcgtc gcggtcggcc tggggaatcg cggcatcgag cagccccgac accgagcggc
     1861 gcatgaggcc gacgccggcc cagacgatgt ggacggctac cgccagcgcc actagcgggt
     1921 ccagcaccag ccagccggtc gcaaatacca ggccgacgcc ggcgaccacg ccgaccgagg
     1981 tccagacgtc ggtcatcagg tgcttgccgt cggcttccag cgtgatgctg cggtgagctt
     2041 tgcccgcgcg cagcagcacc atggccaccg cgagattgat gccggtggcg gcgacgctga
     2101 tggcgaggcc gaggccgggc atcccgagcg gctgcggcga cgccagccgc ggcagcgccg
     2161 tcgcgatgat gcccgctgca gcgagcacga tgagcgcgcc ttcgatgccg gcggagaaat
     2221 actccgcctt cgagaagccg tacgggtggt ccttgtccgg cgctctcgcg gcgaggcgca
     2281 acatggaaag cgcgagcagg gccgcggcca ggttgacgag ggattcgatc gcgtcggaca
     2341 gcagaccgac cgaaccggtc agccaccacg ccagcgtctt gaggcccatc gtggcgagcg
     2401 ccgccgccac cgacagccag gcgtaacgct caagatttgt agagctcgta ggcacctgcc
     2461 gccgcctcgg ccagttcgta cagcgtgatg ctttctcccg aagatgccgc gaatcttcga
     2521 gcctgaagca tcccgggagg agaaaaggct cgttcgtcaa atccgacaat catggctttc
     2581 gtccccggat gaggatcttc gctgccaagc tgctcgcaag cccagcgctt gaagacaaca
     2641 actctgccga ggcaacgctt cgtccagggc gtcgggtttt cgtagcgaga caggtactgc
     2701 ggtgaattga gcaccccagg tgagtccagc tcgtaggttg caagatagct tttttgccaa
     2761 gtccaacggc gtgcagaacg gaagcctggg atagcgaggc gctcggggag gtgctcgtcg
     2821 tcgtaccagg cgttgaactc ctcttccatc gccggcggcg gctcggtgat cgtcagcagc
     2881 agtcctctat tggccatcga tgatccgtcc ccggtcgaga gtataggaac gatccgccag
     2941 gcgccgtgtc aggttcgagc cctgctcggc aagcagcacg gtgacgccgg cgcccttgag
     3001 ctcggcgatc gcgcgcgcga tctgcgccac gaccagcggc gcgagcccct cggagggctc
     3061 gtcgagcaga atcaccctcg ggtttccggc cagcgtgcgg gcgatgcaca gcatctgctg
     3121 ctcgccgccc gacatcagcg cggcgcggcg cccgagcatg ccggcgagcg cggggaacag
     3181 cgccagcagc ctctcctggg tccaagcggc gacacccggc gacgctggcc ggcgtccgac
     3241 ttccaggttt tcctccacgg tcaggtcggt gaagatgcgc cgctcctcgg gaacgtagcc
     3301 caggccggcc cgcgcgattt cataaggctc caggcggtcg atgcgtcggc cgtcgagccg
     3361 cacttcgccg ccgaacgccg gcagcagccc catgacggtc ttgagcgtgg tcgacttgcc
     3421 ggcgccattg cggccgagga gggcgaccac ctcgccggct ccgatctcca ggcgcaagtc
     3481 gaacagcacc tgcgcccggc cgtagccgcc gctcagcccg gcgatctcaa gcagggccaa
     3541 ggtagacctc ccgcacccgc ggatcgtcgc gcacctcgcc gggggcgccg gtggcgatca
     3601 attctccgcc gtgcagcacc agcacgcggt cggcgaagcc gaacaccgcc tccatgtcgt
     3661 gctcggtgaa cagcaccgcg atcttctcgc tgcgcgccag gctcgaagcg aggcccatga
     3721 gcgcgtcgcg ctcctccggc gcggtgccgg cggtgggctc gtccatcagc agcaggcgcg
     3781 ggcggttcgc caaagcgatt gcaagctcga gccgcttgag gtcgccgtag gcgagctcgc
     3841 cacacgggcg cgccgcctgc tgcgccattc cgacgcggcc gagcagctcg tttgcttcct
     3901 cgacgagaag attctctgct ggaaggaaga aggaacccag cagccggtcg cgcgacagca
     3961 gggcgatctg cacgttctcg cgcacgctca tcgatccgaa cgcggctgcc acctggaagg
     4021 tgcgccccac gccgagccgc cagaccgcgc gcggcggcat gccgaccagg ctggtcccgc
     4081 cgagggacac ctctccggcg tccgggcgca gctggccgtt gacgatgttg aagcaggtcg
     4141 tcttgccggc gccgttgggg ccgatcatcg ccaccagctc gccggcgccg acggcgaacg
     4201 agacgccgcg cagcgcctgc acgccgtcgt aggctttcgc cagattgctt acctcgagga
     4261 cggccatttt ttcagcagtc cgacgatgcc ctgcggcagc gccagcacca gcaggagaat
     4321 taccgcgccg agagcagcgc gccagtagtc gacgttacgc gacacgctgt cctcgagcca
     4381 ggtgaagagc gcggcgcccc ataccgggcc ggtgaggctt tgcaggccgc cgagcagcac
     4441 catcaccagg ccgtccaccg agcgcggcac ggagagcgtc tcgggcgaga tcgagccctt
     4501 cgagaaagcg aacagcgcgc cggcgagccc ggcgaacagg cccgcgatgg tgaa
//
LOCUS       DASYJA010000192         1839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2209269, whole genome shotgun sequence.
ACCESSION   DASYJA010000192 DASYJA010000000
VERSION     DASYJA010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2209269"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1839
                     /locus_tag="VGO02_04180"
     CDS             <1..>1839
                     /locus_tag="VGO02_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HEV7476919.1"
                     /translation="NGKVEAVVIYDGIPVPSQHDIPLLRDFLAVHHQLDTSSLAPRDV
                     GVTLARLIKRVRPELDIYLLTDREVEKLAGDPAASMIRRVFYEVEELTEVHLNILEGV
                     GDRFSSPHFDNLKKYAARPIATFHALPIARGKSIMKSNWIRDMGEFYGQNLFLAESSS
                     TAGGLDSMLEPTGNIKIAQEKFARAVGADHVFFVTNGTSTSNKMVYQAVTKPGDIVIA
                     DRNCHKSHHYGMVLSGAQPVYVEAFPMTEYSMYGAVPLRTIKQALLNLKAEGRLDRVR
                     MVTLTNCTFDGHVYNTRRVMEECLAIKPDLIFLWDEAWFGFARWSPFLRPRTGLGAAA
                     ALEEWRSTPEALAAWKAQAAELGEELDPKDDRLMKRRLVPDPRKIRIRVYETDSVHKS
                     MSCLRQGSIVAVRDEDYHHHVSAFREAVFIHASTSPNAQIIASLDVARRQMELEGYEL
                     VMRSTEIALAIRRAVAEHPIISKYFRMLGADAMIPAEFRASGFRDFLTPGSNWLTAVK
                     AMQEDEFFLDPTRMTLVCGSAGFDGTSFKNLLAAKYNIQLNKTSRNSVLLQSNINNTR
                     SDVARRQMELEGYELVMRSTEIALAIRRAVAEHPIISKYFRMLGADA"
BASE COUNT          344 a          596 c          574 g          325 t
ORIGIN      
        1 aacggcaagg tcgaagccgt ggtcatctat gacggcattc cggtgccttc gcagcacgac
       61 atcccgctgc tgcgcgactt tctggccgtc caccaccagc tggatacgtc gagcctcgcg
      121 ccgcgcgatg tcggcgtgac cctggcgcgt ctcatcaagc gagtgcgccc cgagctcgac
      181 atctacctgc tgaccgaccg ggaggtggag aagctcgccg gcgacccggc ggcatcgatg
      241 atccggcgcg tgttctacga agtcgaggag ctgaccgagg tgcacctcaa cattctcgaa
      301 ggcgtaggcg accgattctc ctcgccgcat ttcgacaacc tcaagaagta cgccgcccgc
      361 ccgatcgcca ccttccacgc cctgccgatc gcgcgcggca aatcgatcat gaagtcgaac
      421 tggattcggg acatgggcga gttctacggg cagaacctgt tccttgcgga aagctcttcc
      481 accgccggcg gactggacag catgctcgag ccgaccggca acatcaagat cgcccaggag
      541 aaattcgcgc gcgccgtcgg agcagatcac gtgttcttcg tgaccaatgg cacgtcgacg
      601 tccaacaaga tggtctacca ggcggtgacc aagccgggcg acatcgtgat cgccgaccgc
      661 aactgccaca aatcgcacca ttacggcatg gtgctgtcgg gcgctcagcc ggtgtatgtc
      721 gaagcgttcc cgatgaccga gtactcgatg tacggcgcgg tgccgctgcg taccatcaag
      781 caggcgctgc tcaatctgaa ggccgaggga cgtctcgatc gcgtcaggat ggtgacgctc
      841 accaactgca cgttcgacgg gcacgtgtac aacacccgcc gggtgatgga agagtgtctg
      901 gcgatcaagc cagatctcat tttcctgtgg gacgaggcat ggttcggatt tgcgcgctgg
      961 tcgccgttcc tgcgcccgcg taccggtttg ggcgcggccg ccgcgctgga agagtggcgc
     1021 agcacgcccg aggcattggc ggcgtggaag gcgcaggccg ccgagctcgg cgaggagctc
     1081 gacccgaagg atgatcgtct gatgaaacgg cgcctggtgc cggatccgcg caagatccgc
     1141 atccgcgtct atgagaccga ctcggtgcac aagtcgatgt cgtgcctgcg tcagggctcg
     1201 atcgtggcgg tgcgcgacga ggactaccac caccacgtct cggccttccg cgaagccgtt
     1261 ttcatccacg cctcgacctc gcccaacgcg cagatcatcg cgtcgctgga cgtcgcccgt
     1321 cgccagatgg agctggaagg ctacgagctg gtcatgcgct cgacggagat tgcgctggcg
     1381 atccgccgcg cggtggccga gcacccgatt atctcgaagt acttccgcat gctcggcgcc
     1441 gatgccatga tcccggccga gttccgggcc tcgggtttcc gcgacttcct caccccggga
     1501 agcaactggc ttacggcggt gaaggccatg caggaggacg aattcttcct cgaccccacg
     1561 cgcatgacgc tggtgtgcgg ctctgccggc tttgacggca cttcgttcaa gaatctgctg
     1621 gccgcgaaat acaacattca gctcaacaag acttcgcgca acagcgtgct gttgcagagc
     1681 aacatcaaca acacgcgcag cgacgtcgcg cgtcgccaga tggagctgga aggctacgag
     1741 ctggtcatgc gttcgacgga gattgcgctg gcgatccgcc gcgcggtggc cgagcacccg
     1801 attatctcga agtacttccg catgctcggc gccgatgcc
//
LOCUS       DASYJA010000193         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2117583, whole genome shotgun sequence.
ACCESSION   DASYJA010000193 DASYJA010000000
VERSION     DASYJA010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2117583"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..643)
                     /locus_tag="VGO02_04185"
                     /pseudo
     CDS             complement(125..643)
                     /locus_tag="VGO02_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018127.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
     gene            complement(640..1902)
                     /locus_tag="VGO02_04190"
     CDS             complement(640..1902)
                     /locus_tag="VGO02_04190"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV7476920.1"
                     /translation="MSLHIKNGRVIDPASGRDAAGDVFVADGRIVESLKGKAERTIDA
                     KGLVVAPGFIDLSVRLREPGQEYKATLESEMAAAVAGGVTSLACPPDTDPPLDEPGLV
                     DMLRRRAKALSRARVYPIGALTVKLEGTSLTEMGQLADAGCVAFSQANVPLADTQVLW
                     RALQYAATFGYAVWLRAEDMWLAKGGVAHDGEVATRLGLPGIPPFAETVALGTILELV
                     RATGARVHLCRLSTAGAVELVRRAKAERLPVTCDVGIHHVHLCDVDIGYFDSQCRLEP
                     PLRSQRDRDALARALAEGVIDCMCSDHTPVDEDGKHLPFAEAEPGATGLELLLPLALK
                     WGAAQKLALPASLARITCEPARILGVASGRIAPGAPADLAIFDPQAAVRIAPESLKSQ
                     GKNTPFLGYELAGRVRCTVVAGNVVYEA"
     gene            complement(1902..>2395)
                     /gene="pyrB"
                     /locus_tag="VGO02_04195"
     CDS             complement(1902..>2395)
                     /gene="pyrB"
                     /locus_tag="VGO02_04195"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353628.1"
                     /note="catalyzes the transfer of the carbamoyl moiety from
                     carbamoyl phosphate to L- aspartate in pyrimidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEV7476921.1"
                     /translation="LFTIRHYKKDFTKLTVAIVGDILHSRVARSLIHGLTTLGAPEVR
                     VIGPQTLLPAGVEALGVRVFHDMREGLRGADVVVMLRLQNERMGGALLPSAQEFNKHY
                     GLSAGKLSLAKADAIVMHPGPMNRGVEIDSSVADGPHSVILPQVTFGIAVRMAVMSIV
                     AGN"
BASE COUNT          367 a          828 c          822 g          378 t
ORIGIN      
        1 tgacagacgg cggctgcggc acggcggctt gcgccgcggc gcagaaagcg aacatcagta
       61 gcacataacg caagatcagg tccccgcgaa aaggcgcgaa ttataagtca gcgcggcgct
      121 gcgcctgggc gggaacgacc gcctctatct cgacctgcgc gctgccggcg cctatgtagc
      181 cgagcttgtg cgcggcgacg tacgacaggt cgatgatgcg cccgccgtgg aacggaccgc
      241 ggtcgttgac ccgtaccacc acgctcctgc ggttgctcag gttggtgacc cgcacgtagc
      301 tcgggatcgg cagcgtcggg tgggcggccg tcatcttgta catgtcgtag gtctcgccgc
      361 tcgaggtctt ctggccgtgg aacttcttgc cataccagct cgcggtgccg cgctggctga
      421 acggctgcac cgaagcgagc ggcacgtact tcttgccgaa tacctcatag ggacggttgg
      481 cgtacttgtg cagcggctcg cttctcggcg ccgcgtcggg aatcgaggcg aggttcgccg
      541 gcgggtttgc gccggggccg tcgtccttgt agtacccgcc gcgctgcgga gccgagccgc
      601 agctgatcag caacagagct gaaaaatagg cccagcctct catgcttcgt agaccacgtt
      661 gccggcgacg accgtgcagc gcacgcggcc ggccagctcg tagccgagga acggcgtgtt
      721 cttgccctgg ctcttcagcg actcgggcgc gatgcgcacc gccgcctgcg gatcgaagat
      781 cgcgagatct gcaggcgctc cgggcgcgat gcgcccggaa gcgacgccca ggattctcgc
      841 cggctcgcaa gtgatgcgcg cgaggctcgc cggcagcgcc agcttctgcg ccgcgcccca
      901 cttgagcgcg agcggcagca gcagctcgag tccggtggcg ccgggctcgg cctcggcaaa
      961 cggcaggtgc ttgccgtcct cgtccaccgg cgtatggtcg gagcacatgc agtcgatgac
     1021 gccttccgca agcgcgcgcg ccagcgcatc gcggtcgcgc tggctgcgca gcggcggctc
     1081 gagccggcac tgggagtcga agtagccgat gtcgacatcg cacagatgaa cgtgatggat
     1141 accgacgtcg caggtcacgg gcaggcgctc ggccttcgcg cgccgcacga gctcgaccgc
     1201 gccggcggtc gacaggcggc acaggtgcac ccgggcgccg gtcgcgcgca ccagctccag
     1261 gatggtgccg agcgcgaccg tttcggcgaa cggcggaatc ccgggcagcc cgaggcgcgt
     1321 ggcgacctcg ccgtcatgcg cgacgccgcc tttggcgagc cacatgtcct cggcgcgcag
     1381 ccagactgcg tagccgaacg tggccgcgta ttgcagcgcg cgccacagca cctgcgtatc
     1441 ggcgaggggc acgttcgcct gcgagaaggc gacgcagccg gcatcggcga gctgacccat
     1501 ctcggtgagg ctggtgcctt cgagcttcac ggtcagcgcg ccgatcggat agacgcgcgc
     1561 gcgcgacagc gccttcgcgc gccggcgcag catgtcgacc aggcccggct cgtcgagcgg
     1621 cggatcggtg tccggcgggc aggcgaggct ggtgacgccg cccgccaccg ccgcggccat
     1681 ctcggactcg agcgttgcct tgtactcctg gccgggctcg cgcagccgca ctgagaggtc
     1741 gatgaagccc ggcgcaacca ccaggccttt cgcgtcgatg gttctttccg ccttgccttt
     1801 cagactctcg acgattcttc cgtcggccac gaagacatcg cctgccgcgt cgcgtccgct
     1861 cgccgggtcg atcaccctgc cgttttttat gtgaagagac atcagtttcc ggccacgatg
     1921 ctcatcaccg ccatgcgcac cgcgatgccg aaggtcacct gcggcaggat caccgaatgc
     1981 ggcccgtcgg cgaccgacga gtcgatctcg acgccgcggt tcatcggccc cgggtgcatc
     2041 acgatcgcgt ccgccttggc gagcgacagc ttgccggcgc tcagcccgta gtgcttgttg
     2101 aattcctgtg cggagggcag cagcgcgccg cccatgcgct cgttctgcag ccgcagcatc
     2161 accaccacgt cggcgccgcg caggccctcg cgcatgtcgt ggaacacgcg cacgccgagc
     2221 gcctcgaccc cggccgggag caacgtctgc ggcccgatga cgcgcacctc cggcgcgccg
     2281 agcgtggtga ggccgtggat cagcgagcgc gccacgcgcg agtgcaggat atcgccgacg
     2341 atcgccaccg tgagcttggt gaagtccttc ttgtagtgcc ggatggtgaa cagat
//
LOCUS       DASYJA010000194         3323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1664180, whole genome shotgun sequence.
ACCESSION   DASYJA010000194 DASYJA010000000
VERSION     DASYJA010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664180"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1110)
                     /locus_tag="VGO02_04200"
     CDS             complement(148..1110)
                     /locus_tag="VGO02_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEV7476922.1"
                     /translation="MKKRFWGSLLLCGCAQPQIGLRDMGSFHVGGREVVISGRPVKEV
                     LFTPGGVPAKVDPNGTYLVESMYVQYFLPSQRRGGVPLLMWHGGGLTGVTYETTPDGR
                     EGWLNWFVKKGWDVYNSDAVERGRAGFAMYPDIFKTEPLFLPKNEPFGRFRMQGSEFP
                     VDSYENFVRQVVPRWTSTDDAIIAAYTALVDRVCPCVILFHSQAGQFGFKVAQARPDK
                     VRALIAVEPAGQGDAARAALLKDIPTLFVYGDGIEKDSRWPTIRRNGVAFGEAISKAG
                     GKVEVVDLPKAGIRGNSHMLMMDRNNLEVAALVQAWLEKQGLYK"
     gene            complement(1119..1859)
                     /locus_tag="VGO02_04205"
     CDS             complement(1119..1859)
                     /locus_tag="VGO02_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7476923.1"
                     /translation="MELELKGKTALVTGASMGIGRGIAKALAKEGVRLAVVARRRNLL
                     QELEKEIGAKLVIIEQDFLQEGSPQKIAQAALAGLGTVDILVNNAGGSRRFTLDSTEA
                     QWEEAITLNFTRQRQLTHQLLSQMIARKWGRIINITGKSEPEGINGAFAAKAAMHAWA
                     KGLSREVGKHGITVNSIPPGRIDSEQIRRNYTPEFRQHESEHDIPVGEYGQPEDLANL
                     ACFLASPRARYITGTVIPVDGGLRRYQF"
     gene            complement(1859..3262)
                     /locus_tag="VGO02_04210"
     CDS             complement(1859..3262)
                     /locus_tag="VGO02_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476924.1"
                     /translation="MRWLAMLLILADYGSACAQHGASASNMALLGHQDLQGRSAYQPL
                     VHAQGARWIAYIGHHGSNILNPLTGRREDSGTSIIDVTDPRAPRYLAHIPGEPGEAEQ
                     GGAQMVRVCSGKSLPKGDPAKFYLLRTFGNLAHEVWDVTTPEKPSRIAVVVDKLKGTH
                     KNWWECDSGIAYLVSGLPDWRTRRMTQVFDLSDPAKPVFIRNFGLAGQEPGATGEVPT
                     ELHGPISTGPKGNRVYFGYGTNKSGVMQIVDRQKLLSGPKQPTPANLLAPQVGIFHLN
                     AMTGAHTALPVLGIDVPEFAKDKGGPKRDFVVIVNESLTNECQEPRQMVWMVDITDEK
                     TPVGVANFTVPESHPTSSGGFCSRGGRFGSHSSNENQPPMHAGKTIFVAWFNAGVRAI
                     DIRDPYQPREIGYYIPATTDKTDKRCTKSENATSQGERCKVAIQTNNVDVDDRGYVYI
                     VDRANTGLHILELLKKD"
BASE COUNT          499 a         1110 c         1102 g          612 t
ORIGIN      
        1 ccttcgacat ctcctccgcc gccaggcgcg ccacgatcga cgagctgccg cccggcgcga
       61 aaggcacgag caggcggatg ggtttcgtgg ggtaggtctg cgcgttcgca attcccgcta
      121 aaaaaattac tgttaagaaa acgaatctca tttgtacagg ccctgcttct cgagccacgc
      181 ctggacgagc gcagcgacct cgaggttgtt gcggtccatc atcagcatgt gcgagttgcc
      241 gcggattccc gccttcggca ggtcgaccac ctcgaccttg ccgccggcct tgctgatggc
      301 ctcgccgaag gcgaccccgt tcctgcggat ggtcggccag cgcgagtcct tctcgatgcc
      361 gtcgccatag acgaagagcg tcgggatgtc tttcaggagc gcggcgcgcg ccgcgtcgcc
      421 ctgcccggcc ggctcgaccg cgatcagggc gcgcaccttg tcgggccgcg cctgcgccac
      481 cttgaagccg aattgcccgg cttgcgagtg aaacaggatc acgcacgggc acaccctgtc
      541 gacgagagcc gtgtacgccg cgatgatggc gtcgtcggtg ctggtccagc gcggcaccac
      601 ttgccgcacg aaattctcgt agctgtcgac cggaaactcg ctgccctgca tgcggaagcg
      661 gccgaacggc tcgttcttcg gcaggaacag cggctcggtc ttgaagatgt ccggatacat
      721 cgcgaagccc gcgcggccgc gctccaccgc gtcggagttg tagacgtccc agcccttctt
      781 cacgaaccag ttgagccagc cctcgcggcc gtcgggcgtg gtctcgtagg tgacgccggt
      841 caggccgccg ccgtgccaca tgagcagcgg caccccgccg cggcgctgcg acggcaggaa
      901 gtactgcacg tacatcgatt cgacgaggta ggtgccgttg gggtcgactt tcgccggcac
      961 gccgcccggg gtgaacagca cttccttcac cggccggccc gagatgacga cctcgcgccc
     1021 gccgacgtgg aacgagccca tgtcgcgcag gccgatctgc ggctgggcgc agccacaaag
     1081 caacaatgaa ccccagaaac gcttcttcat ggcagtcctc agaattgata gcgccgcagc
     1141 ccgccgtcga ccgggatcac cgtgccggta atgtagcgcg cgcgcggcga ggcgaggaag
     1201 caggcgaggt tggcgaggtc ctcgggctgg ccgtactcgc ccaccgggat gtcgtgctcg
     1261 ctttcatgct gcctgaattc cggcgtgtag ttgcgccgga tctgctcgct gtcgatgcgt
     1321 cccggcggga tcgagttcac ggtgatgccg tgcttgccca cctcgcgcga caggcccttc
     1381 gcccaggcgt gcatcgcggc cttcgcggcg aacgcgccgt tgatgccctc gggctccgac
     1441 ttgccggtga tgttgatgat gcggccccac ttgcgcgcga tcatctgcga aagcagttgg
     1501 tgggtgagct ggcgctggcg cgtgaagttc agcgtgatcg cctcctccca ctgcgcctcg
     1561 gtgctgtcga gcgtgaacct gcggctgccg ccggcgttgt tgaccaggat gtccaccgtg
     1621 cccaggccgg cgagcgccgc ctgcgcgatc ttctgcggcg agccctcctg caggaagtcc
     1681 tgctcgatga tgacgagctt ggcgccgatt tccttttcca gttcctgcaa gagattacga
     1741 cgacgggcga cgacggcaag gcgcacgcct tccttcgcca gcgccttggc gatgccgcgg
     1801 ccgatgccca tgctcgcgcc cgtgacgagc gcggtcttgc ccttcaattc cagttccatt
     1861 agtccttctt cagaagttcc aggatgtgca ggccggtgtt ggcgcggtcg acgatgtaga
     1921 catagccgcg gtcgtcgacg tcgacattgt tggtctggat cgccaccttg cagcgctcgc
     1981 cctgcgaagt cgcgttttcc gattttgtgc agcgcttgtc ggtcttatcg gtggtcgccg
     2041 gtatgtagta gccgatctcg cgcggctggt acggatcgcg gatgtcgatc gcccgcacgc
     2101 ctgcgttgaa ccaggcgacg aagatcgtct tgccggcgtg catcggcggc tggttctcgt
     2161 tggacgagtg cgagccgaag cgcccgccgc ggctgcagaa gccgccggag gaagtgggat
     2221 gagattcggg cacagtaaag ttggccacgc ccaccggcgt cttctcgtcg gtgatgtcga
     2281 ccatccacac catctggcgc ggctcctggc actcgttggt cagggattcg ttgacgatca
     2341 cgacgaagtc gcgcttcggc ccacccttgt ccttggcaaa ctcgggcacg tcgatgccga
     2401 ggaccggcag cgcggtgtgc gcgccggtca tcgcgttcaa gtggaagatg ccaacctggg
     2461 gggccagcag gttcgccggc gtcggctgct tcggtcccga cagcagcttc tggcggtcga
     2521 cgatctgcat cacgccgctc ttgttggtgc cgtagccgaa gtacacgcgg ttgcccttcg
     2581 gtccggtgga aatgggcccg tgcagctcgg tcggcacctc tcccgtcgcg cctggctcct
     2641 gcccggccag gccgaaattg cggatgaaca ccggcttcgc cgggtcggaa aggtcgaaca
     2701 cctgggtcat gcgccgcgtg cgccagtcgg gcagccccga gacgaggtac gcgatgccgc
     2761 tgtcgcactc ccaccagttc ttgtgcgtgc ccttcagctt gtcgacgacg acggcgatac
     2821 gcgacggctt ctccggagtc gtgacgtccc acacctcgtg cgccaggttg ccgaaggtgc
     2881 gcagcaggta gaacttcgcc gggtcgccct tcggcagcga cttgccgctg cacacgcgca
     2941 ccatctgcgc gccgccctgc tcggcctcgc cgggctcgcc ggggatgtgc gccaggtagc
     3001 gcggcgcgcg cgggtcggtg acgtcgatga tcgaggtgcc gctgtcctcg cgccgcccgg
     3061 taagcgggtt caggatgttg ctgccgtggt gcccgatgta ggcgatccag cgtgcgccct
     3121 gcgcgtgcac cagcggctgg taggcgctgc gaccctgcag gtcctggtgg ccgagcagcg
     3181 ccatgttgga ggccgacgcg ccgtgctgcg cgcaagcgct cccgtagtcc gccaggatca
     3241 gcagcatcgc gagccaacgc atagcactcc tcctagcgcg tgatgctaac cgaagccgcg
     3301 ccgcggcacg cggcttgctg atg
//
LOCUS       DASYJA010000195         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1846539, whole genome shotgun sequence.
ACCESSION   DASYJA010000195 DASYJA010000000
VERSION     DASYJA010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846539"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VGO02_04215"
     CDS             <1..633
                     /locus_tag="VGO02_04215"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HEV7476925.1"
                     /translation="NGFALVPHATRVADFLDDRELRTVYYPEVEALIRQVSGAARVVV
                     FDHTLRSGDEAEREARLLREPVLSAHNDYTEWSGPNRVRELLPDEAEKLLERRFAIIQ
                     VWRAINQPIRSNPLAIADARSVAPEDLLVAERRYPHRIGQTYRLKYNPAHRWFYFPQM
                     RRDEALVFKVFDSAKDGRARFTPHTSFEDPTTPPGAPPRQSIEARALAFF"
     gene            complement(611..1471)
                     /locus_tag="VGO02_04220"
     CDS             complement(611..1471)
                     /locus_tag="VGO02_04220"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7476926.1"
                     /translation="MATPSPLGFIGLGVMGEPMCRNLARKSGRSVVAWDPRQEPLDRL
                     VADGVAAARSLEEVAGKAEIVFLSLPGEKEIREVSRQLRNNVVVDCSTAPVSLAKQLS
                     REIADFADAPVARTRQAAIDGTLSIMVGASEALFARIEPLLRCMASDVTHCGPPGAGQ
                     AVKLLNNMVLSVTVMALAEALAVAKASGAVDPRLLFETLMKGSADSFALRNHGMKALL
                     PETHPEHAFPVTYMMKDVGYALDLARSAGLDLLGASSAMELLTKTKQLGLGEAYHTAI
                     IEAIRKRRAP"
     gene            1470..1829
                     /locus_tag="VGO02_04225"
     CDS             1470..1829
                     /locus_tag="VGO02_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476927.1"
                     /translation="MGAYLATNCLMKPAQLGSLNSRLPSIEEDLAARMDLLFERCPQL
                     HGFTVLDRNLVPGGARVAALERGLFVAGIGIYPELPPDQCERIYDEISVALLDFMVQR
                     PEAREALRGRTFARAFH"
     gene            complement(1823..>2656)
                     /locus_tag="VGO02_04230"
     CDS             complement(1823..>2656)
                     /locus_tag="VGO02_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476928.1"
                     /translation="AEEKGYFRAEGLDYEFRNVFETHAVPDKVGAHTSFAKGRDTNVS
                     CACHWTVNVAASQGHGKLYADAYSVSPAAVFVPPESPIREPQQLAGVPISVGHQSGSH
                     YSTIQALEQYLKPDEIKLSFADGILFGRLEKLVDRQVPAAALFSGPYYFLEQLGFRKI
                     IDTTFMMASMITGNPDPEDVKKYFRALRSAQRDIDLRPELYTHYYRKEFPARFLDQVD
                     TRRWGPGERIVFEPYTRESFEDSFKWVAERKIFDPAQMGSGRYEEAVVSAASWKVSIP
                     Q"
BASE COUNT          416 a          895 c          888 g          457 t
ORIGIN      
        1 aacggcttcg ccctcgttcc acacgccact cgcgtcgccg atttcctcga cgacagagaa
       61 ctaagaacag tctattaccc ggaagtcgag gcgctgatcc gccaggtctc gggcgcggcg
      121 cgcgtggtgg tcttcgatca caccctgcgc tcgggcgacg aggctgagcg cgaggcgcgc
      181 ctgctgcgcg agccggtgct ctccgcgcac aacgattaca ccgagtggtc cggaccgaac
      241 cgcgtgcgcg agctgctgcc cgacgaagcc gaaaagctgc tcgagcgccg cttcgccatc
      301 attcaggtgt ggcgcgcgat caaccagccg atccgctcca atccgctggc catcgccgac
      361 gcgcgctcgg tggcgcccga ggacctgctg gtcgccgagc ggcgctatcc ccatcgcatc
      421 ggccagacct accggctgaa atacaacccg gcccaccgct ggttctactt cccgcagatg
      481 cgtcgcgacg aggcgctggt cttcaaggtg ttcgactcgg cgaaggacgg ccgcgcgcgc
      541 ttcacgccgc acacttcgtt cgaggatccg acgacgccgc ccggcgcgcc gccgcggcag
      601 agcatcgagg ctagggcgct cgcctttttc tgatcgcttc gatgatggcg gtgtggtagg
      661 cctctccgag gcccagctgt ttcgttttag ttagcagttc catggcgctg cttgcgccga
      721 gaaggtccag tcctgcggag cgggcgaggt cgagagcgta gccgacgtct ttcatcatgt
      781 aggtaacggg aaaggcgtgt tccggatgcg tctcggggag aagcgccttc atgccgtggt
      841 tgcgcagcgc gaagctgtcg gccgagcctt tcatcaaggt ctcgaacagc agccgcgggt
      901 cgaccgcgcc gctcgccttc gccaccgcca gcgcttcggc gagcgccatc accgtcaccg
      961 acagcaccat gttgttgagc agcttgaccg cctggccggc gcccggcggg ccgcagtgcg
     1021 tgacgtcgct cgccatgcag cgcaggagcg gctcgatgcg cgcgaagagg gcctcgctcg
     1081 ccccgaccat gatggacagc gtgccgtcga ttgccgcttg ccgcgtgcgc gccacagggg
     1141 cgtcggcgaa gtctgcgatt tcccttgaaa gttgtttggc aagtgagacc ggggcggtac
     1201 tgcagtccac gaccacgttg tttctcaatt gccgggaaac ctcccggatt tccttttctc
     1261 ccggcaacga gagaaagaca atctcggcct tcccggccac ttcctcaagc gagcgcgccg
     1321 cggcgacgcc gtcggcgacc agcctgtcca gcggctcctg acgtgggtcc caagcgacga
     1381 cgctgcggcc cgacttgcgg gcgaggttgc ggcacatggg ctcgcccatg acgcctagtc
     1441 cgatgaaacc aaggggggag ggggtggcca tgggtgcata tcttgcaacc aactgcctca
     1501 tgaagcccgc ccagctaggc agcctcaact cccgcctgcc gtcgatcgag gaggacctgg
     1561 ccgcgcgcat ggacctgctc ttcgagcgct gcccccagct gcacggcttt accgtcctcg
     1621 accgcaacct ggtgccgggc ggcgcgcgcg tcgcggcgct cgagcgcggc ctgttcgtcg
     1681 ccggcatcgg catctacccg gagctccccc cggaccagtg cgagcgcatc tacgacgaga
     1741 tctcggtggc gctgctcgac ttcatggtgc agcgccccga ggcgcgcgag gcgctgcgcg
     1801 ggcggacctt cgcccgcgcg tttcactgag gaatagagac tttccaggag gcggcggaga
     1861 ccaccgcctc ttcatagcgg cccgatccca tctgcgcggg gtcgaagatc ttgcgctccg
     1921 ccacccactt gaacgagtcc tcgaagctct cgcgcgtgta gggctcgaac acgatgcgct
     1981 cgccggggcc ccagcggcgc gtgtcgacct ggtcgaggaa gcgcgccggg aattccttgc
     2041 ggtagtagtg cgtgtacagc tcgggccgca ggtcgatgtc gcgctgcgcg ctgcgcaggg
     2101 cgcggaaata cttcttcacg tcctccgggt cgggattgcc ggtgatcatc gaggccatca
     2161 tgaaggtggt gtcgatgatc ttgcggaagc cgagctgctc gaggaaatag tacggaccgc
     2221 tgaacagggc cgccgccggc acctggcggt cgaccagctt ctccagccgg ccgaagagga
     2281 tgccgtcggc gaacgacagc ttgatctcgt cgggcttgag gtactgctcg agcgcctgga
     2341 tggtcgagta gtggctgccc gactggtggc cgaccgagat cggcacgccg gcgagctgct
     2401 gcggctcgcg gatcggcgac tcgggcggca cgaacaccgc cgccggtgag accgagtaag
     2461 cgtcggcgta cagcttgccg tggccctgcg aggcggcgac gttgaccgtc cagtggcagg
     2521 cgcagctcac gttggtgtcg cgccccttgg cgaagctggt gtgcgcgccc accttgtcgg
     2581 gcacggcgtg cgtctcgaaa acgttgcgga actcgtagtc gagcccctcg gcgcggaagt
     2641 agcccttctc ttcggc
//
LOCUS       DASYJA010000196         3750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_485370, whole genome shotgun sequence.
ACCESSION   DASYJA010000196 DASYJA010000000
VERSION     DASYJA010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485370"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1099)
                     /locus_tag="VGO02_04235"
     CDS             complement(29..1099)
                     /locus_tag="VGO02_04235"
                     /inference="COORDINATES: protein motif:HMM:NF042665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6607 family protein"
                     /protein_id="HEV7476929.1"
                     /translation="MRLLVLLVIIGFAPLGAAETPQFIFAWPLGENALKPRGASTRGA
                     PVVLDSAPSEAWMRLRESRIPPLERDRRAILAMAGPYRVSFDFLEVVRFDPSLKQDAP
                     YQSWATEHVFVAEDRPDFIALQHILAMRVLQGDGKVSEPMVVRHWRQEWRYQADRFLA
                     YEGTNRWAPREVPAAARTGAWVQSVYQVDDSPRYAARGRWEHTDGMSTWISDETWRPL
                     PRREFSVRNDYDVLVGTNRHTITATGWVQEENNLKLVTGDGRYLSREYGIARYERIRD
                     FDFSAAERHFARTEPFWAEVRAAWRELGKSRFTLRAQPDQAQLFMPFFEYAAKLDEGE
                     AFNRDEARAFVKRTLQQSYLTP"
     gene            1182..1922
                     /locus_tag="VGO02_04240"
     CDS             1182..1922
                     /locus_tag="VGO02_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEV7476930.1"
                     /translation="MLAVVCSALNAAEVKGLSTIALQSVLEELVPAFEKANGHKVSVT
                     FGLGMALVKRVQEGESADFVLAPRGGIDGLLKSGKIAAASDATLARSSVGVAVRKGAP
                     KPDISTTDALKRALMDAKSISYSNPAFGGASGVHFAKVLERLGIAEQMKTKTRFPEEG
                     GFTARLLVAGEVDMAIQQVGELLSVAGAELLGPLPGDLQSVTTFAIAVPATASQPDAA
                     KAFIRYLRSPQAAAVMKAKGLDPVEDPR"
     gene            1922..2515
                     /locus_tag="VGO02_04245"
     CDS             1922..2515
                     /locus_tag="VGO02_04245"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7476931.1"
                     /translation="MIVQAPDGADGIEIHVEGDGAETIVMVHGWPDTYRLWDAQVAFL
                     KSRYRCVRFTLPGFDIAKARQACSIDQIAEFLRQVVERVSPGRKVTLLLHDWGCAFGY
                     QFYARFPELVARVVGVDIGDTRGLKHTLSPRAKFLMMAYQWWLSLAWFVGGRLGDRMA
                     LQMKRWMRSRSDERFIGSCMCYPYYLSWFGGRRSYRP"
     gene            2576..2731
                     /locus_tag="VGO02_04250"
     CDS             2576..2731
                     /locus_tag="VGO02_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476932.1"
                     /translation="MFHTQAWIDELALRKENRVIAFDTGHWVMLQQPERFNQAVGGWL
                     SATAVLP"
     gene            2761..3375
                     /locus_tag="VGO02_04255"
     CDS             2761..3375
                     /locus_tag="VGO02_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476933.1"
                     /translation="MRKLVLSAALIAAASVPAFAQKPDAAGAMASKTAPGKGTITNMA
                     RITASVEAIDTAARSVTLKGPRGNVVTLAVGPEVKNFDQVKVGDFVVVRYAEALTLEL
                     KKGGKELRQRSEREGSAAAQPGGRPGAAAGRQITVIADVTAVDRKKQIVTLRGPSRTV
                     DLKLRDPEQIKLVKVGDQVEATYQEAVAVSVEPAPKPKPAAVTK"
     gene            3403..>3750
                     /locus_tag="VGO02_04260"
     CDS             3403..>3750
                     /locus_tag="VGO02_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476934.1"
                     /translation="MGTKAAAAASLACALASPLALGQVVAGDLQKRANGVLALMGYML
                     TPDVTTGSLAISNDATGNPDFGMSTMGGGFTMSGRYPLYLEGTLGYARYDPTFIFNNG
                     QVERSVAAKWDAYS"
BASE COUNT          631 a         1203 c         1306 g          610 t
ORIGIN      
        1 tttccatcgt tcggcgatgc cgaactagct atggcgtgag atagctctgc tgcagcgtgc
       61 gcttgacgaa ggcgcgcgcc tcgtcgcggt tgaatgcctc gccttcgtcc agcttcgccg
      121 cgtattcgaa gaacggcatg aaaagctgcg cctgatcggg ctgggcgcgc agcgtgaagc
      181 gtgacttgcc gagctcgcgc caggccgcgc gtacctcggc ccagaagggc tcggtgcggg
      241 cgaagtggcg ctcggcggcg ctgaagtcga agtcccggat gcgctcgtag cgcgcgatgc
      301 cgtattcgcg cgacagatag cgcccgtcgc ccgtgacgag cttgaggttg ttctcctcct
      361 gcacccagcc ggtggcggtg atggtgtggc ggttggtgcc gaccaggacg tcatagtcgt
      421 tgcgcacgct gaattcgcgg cgcggcagcg ggcgccaggt ctcgtcgctg atccaggtcg
      481 acatgccgtc ggtgtgctcc cagcgcccgc gggcggcgta gcgcggcgag tcgtcgacct
      541 ggtagacgct ctgcacccag gcgccggtgc gggcggccgc gggcacctcg cgcggcgccc
      601 agcggttcgt gccctcgtag gcgaggaagc ggtcggcctg atagcgccac tcctggcgcc
      661 agtggcgcac caccatgggc tcgctgacct tgccgtcgcc ctgaagcacg cgcatcgcga
      721 ggatgtgctg cagcgcgatg aagtcggggc gatcctcggc cacgaagaca tgctcggtgg
      781 cccagctctg gtagggcgcg tcctgcttga gcgagggatc gaagcggacc acttccagga
      841 aatcgaagct gacccggtac ggcccggcca tggcgaggat tgcgcggcgg tcgcgctcga
      901 gcggcgggat gcgcgattcc cgcagccgca tccaggcttc cgacggtgcg ctatccagga
      961 cgacgggcgc gccgcgggtg gatgcgcccc ggggcttgag ggcgttctcg ccgagcggcc
     1021 aggcaaaaat gaactgaggg gtttcggccg ccccgagcgg cgcgaacccg ataatgacca
     1081 gcagaacgag aagccgcatg gcgcgagtgt agcttttcct tcgccgaggt taggatgacg
     1141 gcttcaagga gggggatatg caaagaatag ctgccgctgt agtgctcgcc gtagtctgct
     1201 ccgcgctcaa cgctgcggag gtcaagggcc tgtcgacgat cgcgctgcaa agcgtgctcg
     1261 aggaactggt gcccgccttc gagaaggcta acggccacaa ggtgagcgtc acgttcggcc
     1321 tcgggatggc tttggtgaag cgggtgcagg agggggagag tgccgatttc gtgctggcgc
     1381 cccgcggcgg catcgatggc ctgctcaagt caggcaagat cgccgcggcg tccgatgcca
     1441 cgcttgcgcg ctcgagcgtt ggcgtggcag tgcgcaaagg cgcgccgaag ccggatatct
     1501 ccaccaccga tgcgctgaag agggcgctga tggatgcgaa gtccatcagc tactccaatc
     1561 cggcctttgg cggcgcgagc ggagtccatt tcgccaaggt gctggagcgc ctcggcatcg
     1621 ccgagcagat gaagaccaaa acccggtttc cggaggaggg cggattcacc gcccgcctgc
     1681 tggttgccgg ggaagtcgac atggcaatcc agcaggtcgg cgagctgctg tcggtcgcgg
     1741 gggccgagct gctcggtccg ctgccggggg atctgcaaag cgtcaccacc ttcgccattg
     1801 ctgttccggc gacggcgagc cagccggatg cagcaaaggc gttcatccgc tacctgcgct
     1861 cgcctcaagc cgccgccgtc atgaaagcca agggcctgga ccctgttgaa gaccctcgct
     1921 gatgatcgtc caggcgcctg acggcgccga cggcatcgag attcacgtgg agggtgacgg
     1981 cgctgagacc atcgtgatgg tgcacggctg gccggacacc taccgcctgt gggatgcgca
     2041 ggtcgcgttc ctgaaatcgc gctaccgctg cgtgcgcttc acgctgccgg gcttcgacat
     2101 cgccaaggcg cgccaggcgt gctcgatcga ccagatcgcg gagtttctgc ggcaggtggt
     2161 cgagcgggtg agcccaggcc gcaaggtcac cctgctgctg cacgactggg gctgcgcgtt
     2221 cggctaccag ttctacgccc gcttcccgga actcgtagcg cgcgtcgtcg gggtggatat
     2281 cggcgatacg agaggcttga agcacaccct gtcgccgcgc gcgaaattcc tgatgatggc
     2341 ctaccaatgg tggctctcac tcgcctggtt cgtcggcggg cgcctgggcg atcgcatggc
     2401 gctgcagatg aagcgatgga tgcgcagccg ctcggacgag cgcttcatcg gctcgtgcat
     2461 gtgctacccg tactacctga gctggttcgg cggccggcgc agctatcgcc cgtagttcag
     2521 gcgcttcgcc ccaacctgcc cgctgctgtt catctacggc aagcgcaagc cgttcatgtt
     2581 ccacacccag gcgtggatcg atgagctcgc gctgcgcaag gagaaccggg tgatcgcatt
     2641 cgacaccggc cactgggtga tgctgcagca gcccgagcgc ttcaaccagg ctgtcggcgg
     2701 ctggctctcg gctacggcgg tgttaccctg aacgcccgaa gcaccattca aggaaacacc
     2761 atgcgcaagc tcgtcttatc cgcggcactg attgccgcag catccgttcc cgctttcgcc
     2821 cagaagcccg acgccgccgg cgcaatggcg agcaagaccg cgcccggcaa aggcaccatc
     2881 acgaacatgg ccaggatcac cgcctcggtc gaggcgatcg atacggcggc ccgcagcgtc
     2941 acgctgaagg gcccgcgcgg caatgtcgtc acgctagcgg tcggccccga ggtcaagaac
     3001 ttcgaccagg tgaaagtcgg cgacttcgtg gtggtgcgct atgccgaggc gctgaccctc
     3061 gagcttaaga aaggcggcaa ggaattgcgc cagcgctccg agcgcgaagg cagcgctgcg
     3121 gcgcagcccg gcggacgccc gggcgcggcc gcggggcgcc agatcacggt tatcgccgat
     3181 gtcaccgccg tcgatcgcaa gaagcagatc gtgaccttgc gcggcccgag ccgcacggtg
     3241 gacctgaagc tgcgcgatcc tgaacagatc aagctggtga aggtcggcga ccaggtcgag
     3301 gcgacctacc aggaagccgt tgcagtctcg gtcgagccgg cgccgaagcc gaagccagcc
     3361 gcggtaacca agtagcgtcg cgcggcggga atccagtagc gggtgggaac gaaggcagcg
     3421 gcggccgcgt ccctggcctg cgcgctggct tcgccgcttg cgctcggcca ggtagtggct
     3481 ggcgacctgc agaagcgcgc aaacggcgtg ctcgcgctga tgggctacat gctgacgccg
     3541 gatgtcacca ccggctcgct cgccatctcg aacgatgcga ccgggaaccc cgacttcggc
     3601 atgagcacga tgggcggtgg cttcacgatg agcgggagat acccgctata cctcgagggc
     3661 accctgggct acgcccggta cgacccgacc ttcatcttca acaacggcca ggtcgagcgc
     3721 tcggtcgcgg cgaagtggga cgcctactcg
//
LOCUS       DASYJA010000197         4551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1664391, whole genome shotgun sequence.
ACCESSION   DASYJA010000197 DASYJA010000000
VERSION     DASYJA010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664391"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VGO02_04265"
     CDS             <1..984
                     /locus_tag="VGO02_04265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7476935.1"
                     /translation="VAMPEAYGGAGLGITEAAVVMQAVAQSGAGFSGASAVHMNIFGL
                     NPVVVFGNAEQKKRMLPPLIEGRHKACFAVTEPNTGLNTRNLKVKAEKRNGKYFVSGQ
                     KVWISTAQVAEKVLLLARAEAGLTLFYTDFDRKHIEVREIEKMGRHAVDSNQVFFDGL
                     PVPEADRIGEEGKGFEYILHGMNPERILIAAELVGLGRCAVKRAAAYAKERIVFDRPI
                     GQNQAIQHPLAANWAALEAANLMAFKGAWLYDQGQPCGAEANAAKYLAGEACFDACQN
                     AVMTLGGYGYAREYHVERYLRESFVGRIAPVSPHLILCFIAEKVLGLPKSY"
     gene            990..1817
                     /locus_tag="VGO02_04270"
     CDS             990..1817
                     /locus_tag="VGO02_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEV7476936.1"
                     /translation="MFRFSQVNVFSADPLSGNPLAVVHAAAGLSEARMRALARWTNLS
                     ETTFLLPPTNPDADYHVRIFTPGGELPFAGHPTLGSCHAWLAAGAMPRQPGLVVQQCG
                     VGNVRVRCDGPRLEFAAPPLKRAGALEPDVLAKIAKALSLARSDIVHHQWVDNGPGWC
                     AVMLRSARQVLSLKADWAALDPLKLGVVGPHDAGYDAAFEVRAFIGGGGYEDPVTGSL
                     NASLAQWLIGAGLANSPYVAAQGAALQRAGRVHLRKDGEGVWVGGDVVDVIRGEIDL"
     gene            1892..2644
                     /locus_tag="VGO02_04275"
     CDS             1892..2644
                     /locus_tag="VGO02_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7476937.1"
                     /translation="MAQDTVLETRGLTKEFKGFVAVREVSLKVRRGSIHALIGPNGAG
                     KTTCFNLLTHFLEPTRGQIFFNGREITGSSPSAIARMGLVRSFQISAVFPHLTVLENV
                     RVALQSKVKAGASYDFWISDKSLKKLDEPARELIQAVGLAEFESTVAVELPYGRKRAL
                     EIATTLALDPQMMLLDEPTAGMTHADVERISALIKRVSANRTVLMVEHNLSVVSTLSD
                     RITVLQRGEILAEGDYATVSADPRVKEAYLGA"
     gene            2637..3356
                     /locus_tag="VGO02_04280"
     CDS             2637..3356
                     /locus_tag="VGO02_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7476938.1"
                     /translation="MPDSQPREILLRVKDLHAWYGESHILHGVSFDVHQGEVVTLLGR
                     NGAGKTTTLKSIMGMVARRKGSILFKNTETTNVPSNRIARLGIALCPEERGIFASLNV
                     KENLLLPPQVSEGGLPVEQVFELFPNLRERLYSQGTKLSGGEQQMLAIGRILRTGARL
                     LLLDEPTEGLAPVIVQQIGRILARLKNEGFTILLVEQNFRFAQTVADRHYIVEHGRVV
                     NMVPNDRLEANMDRIHEYLGV"
     gene            3376..>4551
                     /locus_tag="VGO02_04285"
     CDS             3376..>4551
                     /locus_tag="VGO02_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7476939.1"
                     /translation="MHMRKISVAVALALGAAGANAQVSDGVIKIGVLTDMSSLYADLS
                     GPGSVAAARMAVEDFGAAKKGMKVEIISADHQNKPDVGSAIARQWYDVDKVDAIFDTP
                     NSGVALAVNQITRDKGKAFINSGAASSDLTGKACSPNTVHWTYDTWMLANGTGGAIVK
                     TGGDSWFFITADYAFGHALERDTEQVVLKNGGKVLGKVRAPINTQDFSSFLLQAQGSK
                     AKIIGLANAGGDTINSIKQASEFGIVKGGQNLAGLLVFLSDIHGLGLQTAQGLIFTET
                     FYWDLNDQTRAFAKRMAAANNGKYPTMVQAGVYAGVLHYLKAVEAAKTDDGTKVVAKM
                     KELPTDDPLFGKGRIRADGRKVHPAYLVEVKKPAESKGPYDYYKVRTTIPADQAFRPE
                     "
BASE COUNT          805 a         1526 c         1516 g          704 t
ORIGIN      
        1 gtcgcgatgc cggaggctta cggcggcgcc gggctcggca tcaccgaggc ggcagtcgtc
       61 atgcaggcgg tggctcagtc gggggccggg ttcagcggcg cctcggccgt gcacatgaac
      121 atcttcggcc tgaacccggt cgtcgtcttc ggcaacgccg agcagaagaa acgcatgctg
      181 ccgccgctca tcgaggggag acacaaggcc tgctttgccg tcaccgaacc gaacaccggc
      241 ctgaacacgc gtaacttgaa ggtgaaggcc gagaagcgga acggaaaata ttttgtctcg
      301 gggcagaaag tctggatctc gaccgcgcag gtcgccgaga aggtcctgct gctggcgaga
      361 gccgaggcag gcctgacgct gttctatacc gacttcgacc gcaagcacat cgaggtgcgc
      421 gagatcgaga agatgggccg ccacgcggtg gactcgaacc aggtgttctt cgacggcctg
      481 ccggtgcccg aggccgaccg catcggcgag gagggcaagg gcttcgagta catcctgcac
      541 ggcatgaacc cggagcgcat cctgatcgcc gccgagctgg tcggcctggg ccgctgcgcg
      601 gtgaagcgcg ccgccgccta tgcgaaggag cgcatcgtgt tcgaccggcc gatcggccag
      661 aaccaggcga tccagcaccc gctcgcagcc aactgggccg cgctggaagc cgccaacctg
      721 atggccttca agggcgcctg gctctacgac cagggccagc cctgcggcgc cgaagccaac
      781 gccgccaagt acctcgccgg cgaagcctgc ttcgacgcct gccagaacgc cgtgatgacc
      841 ctgggcggct acggctacgc gcgcgagtat cacgtcgagc gctacctgcg cgaatcgttc
      901 gtcgggcgca tcgccccggt gagcccgcac ctcatcctgt gcttcatcgc tgagaaggtc
      961 ctcggcctgc cgaagtccta ctgacgacta tgttccggtt ctcgcaggtc aatgtctttt
     1021 ccgccgatcc gttgagcggg aatccgctcg ccgtggtgca cgccgccgcg gggctgagcg
     1081 aagcgcggat gcgtgcgctc gcgcgctgga ctaatctgtc ggagacgacc ttcctgctgc
     1141 cgccgacgaa tcccgacgcg gactaccacg tgcgcatctt cacgcccggc ggcgagctgc
     1201 ccttcgccgg gcatccgacg ctcggctcgt gccacgcctg gcttgcggcg ggcgcaatgc
     1261 cgcgccagcc ggggctcgtc gtgcagcaat gcggcgtggg caacgtccgc gtgcgctgcg
     1321 acggcccgcg gctcgagttt gccgcgccgc ctctgaagcg cgccggtgcg ctcgagcccg
     1381 acgtgctcgc gaaaatcgcc aaggcgctct cgctggcgcg ttccgacatc gttcaccacc
     1441 aatgggtcga caacggcccg ggctggtgcg cggtgatgct gcgttccgca cgccaggtgc
     1501 tttcgctcaa ggccgactgg gccgcgctcg atcccctcaa gctgggcgtg gtcggcccgc
     1561 acgacgccgg ctacgacgct gccttcgagg tgcgcgcgtt catcggcggc ggcggatacg
     1621 aggacccggt caccggcagc ctcaacgcca gcctcgcgca gtggctcatc ggcgccgggc
     1681 tcgccaactc gccgtatgtc gcggcgcaag gggccgcgct gcagcgcgct ggccgggtcc
     1741 acctgaggaa ggacggcgag ggcgtctggg tcggcggcga cgtcgtagac gtcatccgcg
     1801 gcgagatcga tctctagaga cttcgcgccg gcgcttgccc tgcgggcgcc gagtgcggca
     1861 tcatgccgcc cgcgcagcga ggaggctgct tttggcgcaa gacactgttc tcgaaacacg
     1921 cgggctgacc aaggagttca agggcttcgt cgccgtcagg gaggtctcgc tgaaggtgcg
     1981 gcggggcagc atccatgccc tgatcgggcc gaacggcgcc ggcaagacga cctgcttcaa
     2041 cctgctgacc cacttcctcg agccgacgcg cggccagatc ttcttcaacg gccgcgagat
     2101 taccggctcg agcccttcgg ccatcgcccg catggggctg gtgcgctcgt tccagatctc
     2161 cgcggttttc ccgcacctca cggtgctgga gaacgtgcgg gtggcgctgc agagcaaggt
     2221 gaaggctggc gcttcctatg atttctggat ttcggataaa tcattaaaaa aactggatga
     2281 gcccgcgcgc gagctgatcc aggccgtcgg cctggcggaa ttcgaatcga cggtcgcagt
     2341 cgagctgccc tacgggcgca agcgagcgct cgagatcgcc accacgctcg cgctcgaccc
     2401 gcagatgatg ctgctcgacg agccgacggc cggcatgacg cacgccgacg tcgagcggat
     2461 cagcgcgctg atcaagcgcg tctcggccaa ccgcacggtg ctgatggtcg agcacaatct
     2521 ttcggtggtc tcgacgctgt ccgaccgcat caccgtcctg cagcgcggcg agatcctcgc
     2581 cgagggcgac tatgcgaccg tctcggccga cccgcgcgtg aaggaggcct acctcggtgc
     2641 ctgattctca gcccagggaa atcctgctgc gcgtaaagga tctgcacgcc tggtacggcg
     2701 agtcgcacat cctgcacggc gtgtcgttcg acgtgcacca gggcgaggtg gtgacgttgc
     2761 tgggccgcaa tggcgccggc aagaccacga cgctgaagtc gatcatgggc atggtggccc
     2821 ggcgcaaggg ttctatcctc ttcaaaaata ctgaaacgac aaatgtccct tccaaccgga
     2881 ttgcccgcct gggcatcgcc ctgtgtcccg aggagcgcgg catcttcgcc agcctgaacg
     2941 tgaaggagaa cctgctgctg ccgccgcagg tgagcgaggg cggcctgccg gtagagcagg
     3001 ttttcgagct gttcccgaac ctgcgggagc gcctctacag ccagggcacc aagctttccg
     3061 gcggcgagca gcagatgctc gccatcggcc gcatcctgcg caccggcgcg cgcctgctgc
     3121 tgctcgacga gccgaccgag ggcctcgccc cggtgatcgt gcagcagatc ggccgcatcc
     3181 tcgcgcgcct gaagaacgag gggttcacca tcctgctggt cgagcagaac ttccgcttcg
     3241 cccagacggt ggccgaccgc cactacatcg tcgaacacgg ccgcgtcgtc aacatggttc
     3301 cgaacgaccg gctggaagcg aacatggacc ggatccacga gtatctaggc gtttaaccca
     3361 agcaaggaga agtcgatgca tatgcggaaa ataagcgtgg cagtcgccct cgcgctcggc
     3421 gcggcgggcg cgaacgccca ggtgtccgac ggcgtgatca agatcggcgt tctgaccgac
     3481 atgtcgagcc tgtacgcgga cctgtcgggt ccgggctcgg tcgcagcggc gcgcatggcg
     3541 gtcgaggatt tcggcgcggc gaaaaagggc atgaaggtcg agatcatctc ggccgaccac
     3601 cagaacaagc ccgacgtcgg ctcggccatc gcccgccagt ggtacgacgt ggacaaggtc
     3661 gacgccatct tcgacacgcc caattcgggc gtggcgcttg ccgtcaacca gatcacgcgc
     3721 gacaagggca aggcgttcat caactcgggc gccgcatcgt cggacctgac cggcaaggcc
     3781 tgctcgccga acacggtgca ctggacctac gacacctgga tgctggccaa cggcaccggc
     3841 ggcgcgatcg tcaagaccgg cggcgacagc tggttcttca tcaccgccga ctacgctttc
     3901 ggccacgcgc tggagcgcga caccgagcag gtggtgctga agaacggcgg caaggtgctc
     3961 ggcaaggtgc gcgcgccgat caacacgcag gacttctcct cgttcctgct ccaggcgcag
     4021 ggctccaagg cgaagatcat cggcctggcc aacgccggcg gcgacaccat caactcgatc
     4081 aagcaggcct ccgagttcgg catcgtcaag ggcgggcaga acctcgccgg cctgctggtg
     4141 ttcctgagcg acatccacgg cctggggctg cagacggcgc agggcctcat cttcaccgag
     4201 acgttctact gggacctcaa cgaccagacg cgcgccttcg ccaagcgcat ggccgcggcg
     4261 aacaacggca agtacccgac gatggtgcaa gccggcgtgt acgccggggt gctgcactac
     4321 ctgaaggcgg tcgaggcggc gaagaccgac gacggcacca aggtggtggc gaagatgaag
     4381 gagctgccca ccgacgaccc gctgttcggc aagggacgca tccgcgccga cggccgcaag
     4441 gtgcatccgg cctacctggt ggaagtgaag aagcccgccg agtcgaaagg gccgtacgac
     4501 tactacaagg tgcgcaccac catccccgcg gaccaggcgt tccgaccgga a
//
LOCUS       DASYJA010000198         2313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1846763, whole genome shotgun sequence.
ACCESSION   DASYJA010000198 DASYJA010000000
VERSION     DASYJA010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846763"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VGO02_04290"
     CDS             <1..495
                     /locus_tag="VGO02_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEV7476940.1"
                     /translation="LRLEGVAPSVEARLKGLRELLAASGAKMEELGTLESRAFWCEVR
                     DVAPLAASGDAIIWKISCPPTEGPAIVARIKAQRPAAEAFYDWSGGLIWLALPASADA
                     DYTIVRGAPGSAGGHATLIRAPESVRANVPVFQPQPVALAALASRVKESFDPKRLFNP
                     GRMG"
     gene            535..822
                     /locus_tag="VGO02_04295"
     CDS             535..822
                     /locus_tag="VGO02_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV7476941.1"
                     /translation="MIDESRFRDEVTLRLFYGERVRRYPAALAQRTQNKLKIVYAASR
                     LDDLAVPPGNRLETLKGDRRGQWSIRINDQWRICFAWDGMRATSIEVVDYH"
     gene            835..1143
                     /locus_tag="VGO02_04300"
     CDS             835..1143
                     /locus_tag="VGO02_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEV7476942.1"
                     /translation="MKHKIKPVHPGRILREEFMEPARISQYRLAQATGLSQVQVGNII
                     RGKRGITAETALRLERALGMSAQTWMNLQTLYELEKASTEAGQRIRQTTARLKLREAA
                     "
     gene            1153..>2313
                     /gene="glcF"
                     /locus_tag="VGO02_04305"
     CDS             1153..>2313
                     /gene="glcF"
                     /locus_tag="VGO02_04305"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: protein motif:HMM:NF008434.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEV7476943.1"
                     /translation="MQTNFSLAQLADPETARSENILRKCVHCGFCTATCPTYVLLGDE
                     RDSPRGRIYFIKAMLEGDRAPSETEVRHVDRCLSCLSCMTTCPSGVNYMHLVDHGRHH
                     IETRYTRALPDRLLRGVLAWLMPRPTPFRWAMVLGRFAKPLAALLPAASADPGGATFL
                     RRVRAMLEAVPDRLAGPSPVDRPQTFPAAGLRRKRVALMPGCGQQVLAPEVNEATVRL
                     LTRHDIEVVNVAGSGCCGSSVQHVGRNEQAIALVKRNIEAWLREIDGAGLDAIVINAS
                     GCGTTVKDYGHMLADDPLWKDKAERVARLARDVSEVMLEVGLANVTVRPLTVAYHSAC
                     SMQHGQKVIEQPKKLLRDAGFVVKDVPEGHICCGWAGTYQVLQPELSRRLRDR"
BASE COUNT          405 a          725 c          782 g          401 t
ORIGIN      
        1 ctgcgtctcg aaggcgttgc gccctcggtc gaagcgcgcc tgaaggggct gcgcgagctg
       61 ctggcggcgt cgggtgcgaa gatggaagag ctcggcaccc tcgaaagccg cgccttctgg
      121 tgcgaggtgc gcgacgtggc gccgctcgct gcctcaggcg atgccatcat atggaagatc
      181 tcctgcccgc cgaccgaagg cccggccatc gttgcccgca tcaaggcgca gcgcccggcg
      241 gcagaggcgt tctacgactg gtcgggcggt ctgatctggc tcgccttgcc ggcgagcgcc
      301 gatgctgact acacgatcgt gcgtggcgct cccggctctg caggcggcca cgccaccttg
      361 atccgtgcgc ctgaatcggt gcgagcaaac gtgccggtct tccaaccgca gcccgtcgcg
      421 ctggcggcgc tcgccagccg cgtgaaggaa agcttcgacc ccaagcgcct cttcaatccg
      481 gggcgaatgg gctaaggttg ccgatactaa tcaaagtggt tagtatgcag ccgaatgatc
      541 gatgaaagtc ggttccgcga cgaggttacg ctgaggcttt tctacggcga gcgggtacgt
      601 cgttatccag cagctcttgc ccagcggacg cagaacaaac tgaagattgt ctatgccgca
      661 agtcgcttgg atgatttagc agtgccgccc ggcaatcggc tcgagacctt gaaaggcgat
      721 agacgagggc aatggagcat tcggatcaac gaccagtggc gtatttgctt cgcatgggac
      781 ggaatgagag ccacatctat tgaagttgtg gactatcact gaaaaggcag actgatgaag
      841 cacaagatca agccagtcca tccgggtcgt attctgcgcg aggagttcat ggaaccagcg
      901 cgcatttcgc agtaccggct cgctcaggcg accggactct ctcaggttca ggtcggcaat
      961 atcattcgcg gcaagcgagg cattacggct gagactgcgc tgcggctgga gcgcgcactg
     1021 ggtatgagcg cgcagacctg gatgaatttg cagactcttt atgaactgga aaaggcttcg
     1081 accgaagctg gccagcggat taggcaaaca accgcgcgtc tcaagctacg cgaggcggca
     1141 tagcgctgcc ggatgcaaac gaacttcagc ctggcccagc tcgccgatcc ggagaccgcg
     1201 cgcagcgaga atatcctgcg caagtgtgtg cattgcggct tctgcaccgc gacctgcccg
     1261 acctacgtgc tgctgggtga tgagcgcgac agtccgcgtg ggcggatcta cttcatcaag
     1321 gcgatgctgg agggggaccg cgcgccgagc gagaccgagg tcaggcatgt cgaccgttgc
     1381 ctgtcgtgcc tgtcctgcat gacgacctgc ccgtcgggcg tgaactacat gcacctggtc
     1441 gatcatggcc ggcatcacat cgagacgcgc tacacgcgcg cgctgcccga tcgcttgctg
     1501 cgcggcgtgc tcgcctggct gatgccgcgg ccgacgccgt tccgctgggc catggtgctc
     1561 ggccgcttcg ccaagccgct ggcggcgctg cttccggccg cgagcgccga tcccggcggc
     1621 gccaccttcc tgcgccgggt gagggcgatg ctcgaggcgg tgcccgatcg gctcgccggg
     1681 ccttcgccgg tcgaccggcc gcagactttc ccggcggccg ggcttcgccg caagcgcgtg
     1741 gcgctgatgc cgggctgtgg ccagcaggtt ctggcgcccg aggtcaacga ggccacggtg
     1801 cggctgctga cgcgccacga catcgaggtg gtgaacgtcg cgggctcggg ctgctgcggc
     1861 tcctcggtcc agcatgtcgg ccggaacgag caggcgatcg cgctcgtcaa gcgcaacatc
     1921 gaggcgtggc tgcgcgagat cgacggcgcc ggcctcgacg ccatcgtcat caacgcctcg
     1981 ggctgcggaa cgacggtgaa ggattatggc cacatgctgg ccgacgatcc gctgtggaag
     2041 gacaaggccg aacgggtggc ccgcctcgcc agggatgtca gcgaggtcat gctcgaggtc
     2101 ggtctcgcga acgtgaccgt gcggccgctc accgtggcgt atcattcggc ctgttcgatg
     2161 cagcacggcc aaaaagtgat cgagcagccc aagaagctgc tccgcgacgc gggcttcgtc
     2221 gtcaaggacg tgcccgaggg acatatttgt tgcggctggg ccggcaccta ccaggtgctg
     2281 caacccgagc tgtcgcgccg cctgcgcgat cgc
//
LOCUS       DASYJA010000199         1811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3118192, whole genome shotgun sequence.
ACCESSION   DASYJA010000199 DASYJA010000000
VERSION     DASYJA010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3118192"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VGO02_04310"
     CDS             <1..450
                     /locus_tag="VGO02_04310"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7476944.1"
                     /translation="QDRTKKYYLKNLCGNPYDTGIAAAHLIFGGVLDRFPNLEFNLPH
                     AGGTMPSLIGRWDHGTKVRPELKHMKRPPSEYLRRYTYDTIGHDDRINANLVRLVGAD
                     RVTLGSDYCFDMGLDDPLATVNRIAGISEDEKKLIQGKNAARLLRLG"
     gene            443..772
                     /locus_tag="VGO02_04315"
     CDS             443..772
                     /locus_tag="VGO02_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476945.1"
                     /translation="MASLLAACSDPAAPPPSTPPAQAYYQDYVVADRPPGFTLRNQIE
                     PTRADPKRFYWRATYDREGRVVTLETFAYPMCLQSQLILVYDAKGTAPLKRQRQPAAD
                     CKPARSF"
     gene            complement(783..>1811)
                     /locus_tag="VGO02_04320"
     CDS             complement(783..>1811)
                     /locus_tag="VGO02_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV7476946.1"
                     /translation="LAAGDRDVSRYIDAVSRSSDRAASLTQRLLAFARRQPLDPKPLD
                     ANKLVAGAVDMLRRSLPESIAMETVLAGGLWWTQADPNQLESALLNLALNAKDAMPAG
                     GELTIETANAFLDETYAAAHSEVTPGQYVLIAVSDSGTGMQKEVAARAFEPFFTTKDE
                     GQGTGLGLSQVYGFIKQSRGHVKLYSEPGGGTSVKIYLPRLDASPLVEAAVEVKPQPA
                     APVRETILLVEDDEDVRGFIADSLRELGYQVLAAGDGHAALQALAEKPDIDLLFTDVG
                     LPNGMNGRALADEARRRRPRLKVLFTTGYARNAIIHHGRLDAGVDLITKPYTQTELDA
                     KVRKVLDR"
BASE COUNT          291 a          612 c          600 g          308 t
ORIGIN      
        1 caggatcgca cgaagaagta ctacctgaag aatctctgcg gcaatccgta cgacacgggt
       61 atcgcggcgg cgcatctcat tttcgggggt gtgctggacc ggtttccgaa cctggaattc
      121 aacctgccgc atgcgggcgg aaccatgccg agcctgatcg gccgctggga ccacggcacc
      181 aaagtgcggc cggaactgaa gcacatgaag cgcccgccga gcgaatacct gcggcgctac
      241 acctacgaca cgataggcca cgacgaccgc atcaacgcca acctggtgcg cctggtcggc
      301 gccgaccgcg tcacgctggg cagcgactac tgcttcgaca tggggctgga cgatccgctg
      361 gcaacagtga acagaattgc gggaatctcc gaagatgaaa agaagctcat tcagggaaag
      421 aatgcggctc gccttctgcg ccttggctag cctgctggcg gcctgctccg acccggcggc
      481 gccgcctccc agcacgccgc ccgcccaggc gtactaccag gactatgtgg tcgccgatcg
      541 gccgcccggc ttcacgcttc gcaaccagat cgagccgacg cgcgccgatc cgaagcgctt
      601 ctactggcgc gccacctacg accgcgaagg gcgcgtcgtc acgctggaga ccttcgccta
      661 tccgatgtgc ctgcagtcgc agctcatcct cgtctacgac gcgaaaggga cggcgccgct
      721 gaagcggcag cggcagccgg cggccgactg caagccggcg cggtcgttct gagggcgggc
      781 tgctagcggt ccagcacctt gcgcactttc gcgtccagct cggtttgcgt gtacggcttg
      841 gtgatcaggt cgactcccgc gtcgaggcgg ccgtgatgga tgatcgcatt gcgcgcatag
      901 ccggtcgtga agagaacctt caggcgggga cggcgcctgc gcgcctcgtc ggcgagcgcg
      961 cgcccgttca tgccgttcgg caagccgaca tcggtgaata gcagatcgat gtccggtttc
     1021 tctgccaggg cctgcagcgc ggcatgcccg tcgcccgccg cgagcacctg gtagccgagc
     1081 tcccgcaggg agtctgcgat gaagccgcgc acgtcctcgt catcctcgac taggaggatc
     1141 gtttcgcgca ccggcgccgc cggctgcggc ttcacttcga ccgcggcttc gaccagcggc
     1201 gacgcgtcga gcctcggcag gtagattttt acgctggtgc cgccgccggg ctcgctgtac
     1261 agcttgacgt ggccgcgcga ctgcttgatg aagccataga cctgcgaaag cccgaggccg
     1321 gtcccctggc cctcgtcctt ggtcgtgaaa aacggctcga aggctcttgc tgcgacctcc
     1381 ttctgcatcc cggtgccgct gtcgctgacc gcgatcagca cgtactgtcc cggcgtgacc
     1441 tcgctatgcg cggcggcgta ggtctcgtcc aggaacgcgt tggcggtctc gatcgtaagc
     1501 tcgccgcctg cgggcatcgc gtccttggcg ttgagggcga gattcaggag ggcgctttcc
     1561 agctggttgg ggtcggcctg ggtccaccac aagccgcccg ccagcactgt ttccatcgcg
     1621 atcgactccg gcaggctgcg ccgcagcatg tcgaccgcgc cggcgacgag cttgttcgca
     1681 tcgagcggct tggggtccag cggctggcgg cgcgcaaacg cgagcagccg ctgtgtcagg
     1741 ctcgcagccc ggtccgagct gcggctgacg gcgtcgatgt agcggctgac gtcgcggtcg
     1801 ccggcggcca a
//
LOCUS       DASYJA010000200         3083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_849397, whole genome shotgun sequence.
ACCESSION   DASYJA010000200 DASYJA010000000
VERSION     DASYJA010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849397"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VGO02_04325"
     CDS             <1..690
                     /locus_tag="VGO02_04325"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEV7476947.1"
                     /translation="VTSVAFAVNPSLYENAGYDPFKDFAPVILGPSTPNIITVNPAVP
                     AKNLQELIELARKEKLSYASSGIGTTTHLSMERIKTIAKVDITHVPYQPAQAVGAAVA
                     GHTQISSTSLPPAVTHVRGGRVRAIAVTSAQRSPALPDVPTVNEQGFAGFDDLTWIGF
                     FAPAGMQQDLVNRINSEITRALAAPDVAERLAQLGLEWKPNTSAEFAAFLRGEVQKWA
                     KAVKDSGAKAD"
     gene            690..1742
                     /locus_tag="VGO02_04330"
     CDS             690..1742
                     /locus_tag="VGO02_04330"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEV7476948.1"
                     /translation="MGPFRADQVGSLLRPYGLSEARKKGDLKFQRNAIREVVARQEAV
                     GLQAVTDGEFSRDWWHLDYMLQLEGVSAKANPGPKFGGTEEQPPIPNVTGKLRYAKPI
                     MVDDFAFLKSCTSRAAKFTIPSPSMLHLRAGRAGISRQVYPDIDEFWDDAAQAYRASI
                     ADFARAGCTYLQLDDVSFSYLCDPKIRDNCRKNGDDPDALPRTYADTITRALQGRPAG
                     MTVTMHTCRGNFKSAWVAEGGYDPVAEAMFSSGVDAFFMEFDSARSGGFEPLRFLPKG
                     KKVVLGLVSSKVGVLEKKDDIKKKIDEAAKFVPLENLCLSPQCGFSSTHHGNNLSPDE
                     QWRKLELVVQIANEVW"
     gene            1739..>3083
                     /locus_tag="VGO02_04335"
     CDS             1739..>3083
                     /locus_tag="VGO02_04335"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEV7476949.1"
                     /translation="MTQFKGRREDDRLLTGQGRYTADWNLPEQLYAYFLRSDRAHARI
                     VSLHREEIPGVTVFTGEDTKELKTPPPMLKIPGRGEALKVPHRPTLAQGRVRFVGETV
                     ALVVADSPQAAQDAAEMIEVEYQDLPAVVSEKEALQAGAPLLHDAILGNLAFDYDYGD
                     EKAVDEVFARAAHVVRVTLDSTRVSGNPMEPRSCVAAWDAANGRFDVYCSSQGMSMML
                     PALQATLGMPAGSIRVHARDVGGGFGVRSQPYPEYGAVMVAARKLGRPVKWVGSRFES
                     LASDHHGRGAELSGALALDQDGRFIGLRFDWICNLGAYASQAGPMINTVNPAMHAINA
                     YDIRALYGRKKLVYTNTTPTTAYRGAGRPNVTYLVERLVEEAARQSGIDPIELRRRNL
                     IPKSAHPYKTPTGSIYDSGDFPALLEKAVEFSSWKSFEERRKESKSKGKLRGIGCA"
BASE COUNT          578 a         1019 c          997 g          489 t
ORIGIN      
        1 gtgacgagcg tcgccttcgc agtcaacccg tcgctctacg agaacgccgg ctacgacccg
       61 ttcaaggact tcgcgccggt gatcctcgga ccgagcacgc cgaacatcat caccgtcaac
      121 cccgcggtgc cggcgaagaa cctgcaggag ctgatcgagc tggcgcgcaa ggagaagctg
      181 tcctacgcct cttccggcat cggcaccacc acgcacctgt cgatggagcg catcaagacc
      241 atcgcgaagg tcgacatcac ccatgtcccg taccagccgg cgcaggcggt cggcgccgcg
      301 gtcgccggcc acacgcagat ttccagcact tcgctgccgc ccgccgtgac gcacgtgagg
      361 ggcggcaggg tgcgcgccat cgccgtgacc agcgcgcagc gctcgccggc cctgcccgac
      421 gtgccgacgg tgaacgagca gggcttcgcc ggcttcgacg acctgacctg gatcggtttc
      481 ttcgctccag cgggcatgca gcaggacttg gtgaaccgca tcaattccga gatcacccgc
      541 gcgctcgccg cgccggatgt ggccgagcgc ctggcgcagc tcggcctcga gtggaagccg
      601 aatacctccg ccgagttcgc cgcgttcctg cgcggggaag tgcagaagtg ggcgaaggcg
      661 gtgaaggatt ccggcgcaaa agcagactga tgggaccgtt tcgagcggat caggtgggaa
      721 gtcttttgcg gccgtacggt ctctccgagg ctcggaagaa gggagatctc aagttccagc
      781 ggaacgccat tcgtgaagtg gttgccaggc aggaagccgt agggctgcaa gcggttacag
      841 atggagaatt cagtcgggac tggtggcacc tcgactacat gctgcagctc gagggcgtga
      901 gcgccaaggc gaatccgggg ccgaagttcg gcggcaccga ggagcagccg ccgatcccca
      961 acgtgaccgg caagctgcgc tacgcaaaac cgatcatggt tgatgatttt gcatttctaa
     1021 aatcctgtac ttccagggcg gcgaagttca ccatcccctc tccctccatg ctccacctgc
     1081 gcgccgggcg cgcgggaatc tcgcgccagg tctatccgga catcgacgag ttctgggacg
     1141 acgcagcgca ggcctaccgc gcctcgatcg ccgacttcgc aagagccggc tgcacctacc
     1201 tgcagctcga cgacgtctcg ttctcctacc tgtgcgaccc gaagatccgc gacaactgcc
     1261 gcaagaacgg cgacgacccg gacgcgctgc cgcgcaccta tgccgacacc atcacccggg
     1321 cgctgcaagg caggccggcg gggatgacgg tcacgatgca tacctgccgc ggcaacttca
     1381 agagcgcctg ggtcgccgaa ggcggctacg acccggtggc cgaggccatg ttctcgagcg
     1441 gcgtcgacgc cttcttcatg gagttcgact cggcccgctc gggcggcttc gagccacttc
     1501 gcttcttacc caaagggaag aaggtggtgc tgggcctggt ttcctccaag gtgggagtcc
     1561 tcgagaagaa ggacgacatc aagaaaaaaa tcgacgaggc ggcgaagttc gtgccgctgg
     1621 aaaacctctg cctgtcgccg cagtgcggct tctccagcac gcaccacggc aacaacctga
     1681 gccccgacga gcagtggcgc aagctcgagc tcgtggtcca gatcgcaaac gaggtctggt
     1741 gacgcagttc aaggggcgcc gggaagacga ccgtctcctc accgggcagg gacgctacac
     1801 agccgactgg aatctgccgg agcagcttta cgcgtacttc ctgcgctcgg accgagcgca
     1861 cgcccggatt gtttcccttc atagagaaga aattcccggc gtcacggtat ttaccggcga
     1921 ggacaccaag gaactgaaga caccgccgcc gatgttgaag attcccggac gcggcgaggc
     1981 cctcaaggta ccgcaccgtc cgacgctcgc gcagggccgc gtgcgcttcg tcggcgaaac
     2041 ggtcgcgctg gtggtcgccg attcgccgca ggcggcgcag gacgcagccg agatgatcga
     2101 ggtcgagtac caggacttgc ccgccgtcgt ttccgagaag gaagcgcttc aggcgggggc
     2161 tccgctgctg cacgacgcaa tcctgggcaa tctcgccttc gactacgact acggcgacga
     2221 gaaggccgtc gacgaggtgt ttgcgcgcgc ggcgcatgtc gtgcgcgtca cgctcgattc
     2281 gacccgcgtg tccggcaatc cgatggagcc gaggtcctgc gtcgctgcgt gggacgcggc
     2341 gaacggccgg ttcgacgtct attgcagctc gcagggcatg tcgatgatgc tgccggcgct
     2401 gcaggcaacc ctcggcatgc ctgccgggag catccgcgtg cacgcgcgcg atgttggcgg
     2461 cggcttcggc gtgcgctcgc agccctatcc cgaatacggc gcggtgatgg tcgccgcgcg
     2521 caagctcggc cggccggtga agtgggtcgg ctcgcgcttc gagagcctcg ccagcgacca
     2581 ccacggccgc ggcgccgagc tttcgggcgc gctcgcgctc gaccaggatg ggcgcttcat
     2641 cggcctgcga ttcgactgga tctgcaacct gggcgcctat gcctcgcagg ccgggccgat
     2701 gatcaatacg gtgaacccgg cgatgcacgc gatcaacgcc tacgacatcc gcgcgctcta
     2761 cggccgcaag aagctcgtct atacgaacac cacacccacg accgcgtatc gcggcgccgg
     2821 gcggccgaac gtgacctacc tcgtcgagcg cctggtcgag gaggccgctc gccagtcggg
     2881 catcgacccg atcgagctgc gccggcgcaa cctgatcccg aaatcggccc atccgtacaa
     2941 gacgcccacc ggctccatct acgacagcgg cgattttccc gccttgctcg agaaagcggt
     3001 tgaattttct tcgtggaaaa gttttgagga aagaaggaaa gaatcaaaga gcaaaggcaa
     3061 gctgcgcggc atcggctgcg cca
//
LOCUS       DASYJA010000201         1590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1301846, whole genome shotgun sequence.
ACCESSION   DASYJA010000201 DASYJA010000000
VERSION     DASYJA010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301846"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1491
                     /locus_tag="VGO02_04340"
     CDS             <1..1491
                     /locus_tag="VGO02_04340"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEV7476950.1"
                     /translation="YGCRVPSMGLALLVLWPVLYTDVTETWKLASRRQRLAVGLAGVT
                     AELVFAIFATLAWSFLPDGPLRGAAFILATSTWISTLLLNLSPFMRFDGYFVLSDWLE
                     MPNLHARAFAIARWWLRETLLGLGDPPPEELPAGRRRFLVAFAFATWTYRVVLFLGIA
                     VLVYHFAIKALGIAMGLVELGYFLLRPLWLEARALWKLRDRLRWGLRPAATLGGLLAI
                     VLLLAVPWRSSVEAPALLRSAAFEAVFAPAAGSQVTAVLTHNGAEVAKGASLVSLASP
                     DLAYKLQQAKADLQLVDWQLSMQSVVPDLLARSLVTAHEYEAALAAYRSLADETARLE
                     VKAPIAGRVVDLAEDLRAGDWVPANERLLAVMQPDRAMIEAYVSEADLARIGVGTPAA
                     FYAEGDWREAFAAHVTRIERASARILAEPYLVSRFGGAIAVREMKAGEWVPENAVYRV
                     MLAPDTPEAAPARVMRGRIVLEGASESLAARAWRRIVAVAVRESGL"
BASE COUNT          199 a          567 c          537 g          287 t
ORIGIN      
        1 tatggctgcc gcgtgcccag catgggcctc gcgctcctgg tgctgtggcc ggtgctctac
       61 accgacgtga cggagacctg gaagctcgcc tcgcgccgcc agcggcttgc ggtcggtctc
      121 gccggcgtga cggctgagct cgtcttcgcc atctttgcca ccctcgcctg gagctttctg
      181 cccgatgggc cgttgcgcgg cgcggccttc atcctcgcca ccagcacctg gatctcgacg
      241 ctgttgctca acttgagccc gttcatgcgc ttcgacggct acttcgtgct gtccgactgg
      301 ctcgagatgc ccaacctgca tgcgcgcgct ttcgccatcg cgcgctggtg gctgcgcgaa
      361 acgctgctcg ggctgggcga cccgccgccc gaagagctgc cggccggccg tcggcgcttc
      421 ctggtcgcct tcgccttcgc cacctggaca taccgtgtcg tgctgttcct gggcatcgcg
      481 gtgctcgtct accatttcgc catcaaggcg ctcggcatcg ccatgggctt ggtcgagctc
      541 ggctatttcc tgctgcggcc gctatggctc gaagcgcggg ccctctggaa gttgcgcgac
      601 aggctgcgct ggggcttgcg gcccgccgct acgctcggcg gtctcctcgc catcgtgttg
      661 ttgctggccg tgccatggcg cagctcggtc gaggcgccgg cgctcctgcg cagcgcggcc
      721 ttcgaggcgg tgttcgcgcc ggccgcgggc tcgcaggtca ccgccgtgct gacgcacaac
      781 ggcgccgagg tggcgaaggg cgcgtcgctc gtcagcctcg cctcgcccga tctcgcctac
      841 aagctccagc aggcgaaggc cgacctgcag ctcgtcgact ggcagctctc gatgcagagc
      901 gtcgtccccg acctgctggc caggagcctc gtcaccgcgc acgagtacga agcggccctc
      961 gccgcctatc gcagccttgc cgacgagacg gcacggctgg aggtgaaggc gccgatcgcc
     1021 ggccgcgtcg tcgacctcgc cgaggatctg cgcgccggcg actgggtacc ggccaatgag
     1081 cggctgctgg ccgtcatgca gcccgatcgc gccatgatcg aggcctatgt ctcggaggct
     1141 gatctggcgc ggatcggcgt cggcacgccc gccgccttct atgcggaggg cgactggagg
     1201 gaggctttcg ccgcgcacgt aacgcggata gagcgcgcca gcgctcgcat tctcgccgag
     1261 ccctacctcg tctcgcgttt cggcggcgcg atcgccgtgc gcgaaatgaa agccggcgag
     1321 tgggtgcccg agaatgccgt ctatcgcgtc atgctcgcgc ccgatacgcc cgaggcggcg
     1381 ccggcgcggg tgatgcgcgg ccgcatcgtc ctcgagggcg cctcggagag cctggccgct
     1441 cgcgcctggc ggcggatcgt cgccgtagcg gtgcgcgaaa gcggactcta aactcatgtt
     1501 cgtcgaaccc gattcaccag gttgttgatc tcgatgtcac gcgacaagcg actgcaggac
     1561 cgctcggtct atccgcactg gtgcaccgac
//
LOCUS       DASYJA010000202         3646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2390799, whole genome shotgun sequence.
ACCESSION   DASYJA010000202 DASYJA010000000
VERSION     DASYJA010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2390799"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VGO02_04345"
     CDS             complement(<1..492)
                     /locus_tag="VGO02_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hydroxyacyl-ACP dehydratase"
                     /protein_id="HEV7476951.1"
                     /translation="MGAFLPLLAGFGAQDRVGWSAGAAVSAAQFCAAALELAGQLPRK
                     RFALNLCVDRLSFALGIAAALIARQTSLLPPSSAAGALRDLQCAHPDSYCIADRAELP
                     AGLPAILVPPWRPAAGGFDIPAIPASHIAYTAFTSGSTGRPQPHSRTWGSLVTAARLL
                     DLGL"
     gene            complement(492..971)
                     /locus_tag="VGO02_04350"
     CDS             complement(492..971)
                     /locus_tag="VGO02_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476952.1"
                     /translation="MPRVRRLLTAAAFLAFPLLCHTAVERGNALAAGFGIALPLAVNL
                     ALCALFGRTLATGREPMIARFARLQRGAELPPDLASYARTLTVLWTWFFASMAAISLG
                     LAIWGSTLAWSLFTNVVNYLLVAAFFVGEYVYRRVRFKHYRHPSPIEVARGLHLYRP"
     gene            complement(982..1257)
                     /locus_tag="VGO02_04355"
     CDS             complement(982..1257)
                     /locus_tag="VGO02_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEV7476953.1"
                     /translation="MAAVMQQSALEGEVAKLIVDAVGLDLPAADIDPTQPLFASGLGL
                     DSIDLLEIALAISGTYGFQLRSDDPDNERIFASLRALSEHVARQRKR"
     gene            complement(1278..>3646)
                     /locus_tag="VGO02_04360"
     CDS             complement(1278..>3646)
                     /locus_tag="VGO02_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476954.1"
                     /translation="VWLLCRPLHLSPVRGDSRAESATLLLAVAFFTGLASFVYEIVWI
                     RMLSLVLGASTHAFELMLATFISGLALGGLAIRRRVDASPAPERLLGWAQVAMALAAA
                     ATLPVYDFTFDLMEALMAGLARNNTGYLLFNLSGAAIAALVMLPATFCAGMTLPLLAG
                     ALLRRGAGEAAIGRVYAANTLGAIAGVVLAVHAGLPLLGLKFTLLAGCLVDAVLGLVL
                     LNFFSHRQFIWAAAASAAILVAIGAGVQLDPHKMTSGVFRHGELATSRDARILFAKDG
                     KTATVHLVQYADATSLRTNGKSDGSIVLDRDLTRGSDEITMVLTAAVPLALKPDTRRA
                     AVIGIGTGLTTHTLLQSLAIEHVDTVEIEAAMAEAARGFMPRNSGAFADPRGSILIDD
                     AKSFFSASGARYDLIISEPSNPWVSGVASLFTREFYRRIRGHLEPGGLLVQWFQLYEI
                     DASLVASVMGALGEEFPHYAVFAPSDHDLLIVAGSRELPLPPQARVFEHPGLAKELWT
                     VHVLSAGDLDARYLGGRGLLEPLFASYGVAANSDYAPLLDLNAARHRFTERSATDLVA
                     LLNLEVPVLEMLEPARSRRPVNPLFQGAYAFDRIENARLAWYARDFLIGARRPAPESV
                     PTQLQKDLDLLRVRLIECRDPRDFDVWIHSALRVAALVNPHLAADDASAIWTRVQASH
                     CYASLSGLQREWISLLHAVAARDAARMAAIAEGILTTEAGLAIEAREYLLMSAMTGRL
                     ASRESAQALRLWQEHAERVRASAPAFRLLRCHAESAGCAAQFAPYAER"
BASE COUNT          590 a         1223 c         1371 g          462 t
ORIGIN      
        1 caggccaagg tcgagcagcc gcgcggcggt caccagcgag ccccacgtgc gcgagtgcgg
       61 ctgcggccgg ccggtgctgc ccgaggtgaa cgcggtgtag gcgatgtggg aggcgggaat
      121 cgcgggaatg tcgaagccgc ccgcggccgg gcgccagggc ggcaccagga tcgccggcag
      181 gcccgccggc agctcggcgc ggtccgcgat gcagtagctg tcgggatgcg cgcactgcag
      241 gtcgcgcaac gcaccggcgg cgctcgacgg cggcagcagg ctggtctggc gcgcgatcag
      301 cgccgcggca atgccgagcg cgaagctcaa ccggtcgacg cacaggttga gcgcgaagcg
      361 cttgcgcggc agctggccgg cgagctcgag cgccgcggcg cagaactgcg ccgcgctcac
      421 cgcagcgccg gcgctccagc cgacgcggtc ctgcgccccg aagccggcca gcagaggcag
      481 gaaggcgccc atcacggccg gtacagatgc aatccgcgcg ccacttcgat cggactgggg
      541 tgccggtagt gcttgaagcg caccctgcgg tagacgtatt ccccgacgaa gaacgcagcc
      601 accagcaggt aattgacgac gttggtgaag agcgaccagg cgagcgtcga accccagatc
      661 gcgaggccga gcgagatcgc cgccatcgag gcaaagaacc aggtccagag gacggtgagc
      721 gtgcgcgcgt agctcgccag gtcgggcggc agctcggcgc cgcgctgcag gcgcgcaaag
      781 cgcgcgatca tcggctcgcg tccggtggcg agcgttcggc cgaacagtgc gcacagcgcg
      841 aggttcaccg cgagcggcag cgcgatgccg aatcccgccg cgagcgcgtt accgcgctcg
      901 acggcagtgt ggcagaggag cggaaaggcg agaaacgccg cagcggtcag aaggcgcctg
      961 acgcgcggca ttaccgcccg ctcaccgctt gcgctggcgc gcgacgtgct cgctcagcgc
     1021 gcgcagcgag gcgaagatgc gctcgttgtc ggggtcgtcg gagcgaagct ggaagccgta
     1081 cgtgccgctg atggcgagcg cgatctcgag caggtcgatc gagtccaggc cgaggcccga
     1141 ggcgaacagc ggctgcgtcg gatcgatgtc ggcggccggc aggtcgaggc ccaccgcgtc
     1201 gacgatcagt ttcgcgactt ccccctcgag cgcgctctgc tgcatcactg cggccatttc
     1261 gccggcgatt atagcgctca gcgctcggcg tacggcgcga attgcgccgc gcatccggcg
     1321 ctttcggcgt ggcagcgcag caggcggaac gcgggcgccg aggcgcgcac gcgctcggcg
     1381 tgctcctgcc aaaggcgcag cgcctgcgcg gactcgcgcg acgcgaggcg gccggtcatc
     1441 gccgacatga ggaggtattc gcgcgcctcg atggcgaggc ccgcctcggt ggtcaggatg
     1501 ccctccgcga ttgccgccat gcgcgccgca tcgcgcgcgg cgaccgcgtg cagcagcgag
     1561 atccactcgc gctgcaagcc gctcagcgac gcgtagcagt ggcttgcctg cacccgcgtc
     1621 cagatcgcgc tggcgtcgtc ggccgcgaga tgcgggttga ccagcgcggc gacccgcagc
     1681 gcgctgtgga tccagacgtc gaagtcccgc ggatcgcggc attcgatgag ccgcacgcgc
     1741 agcaggtcaa ggtccttctg cagctgcgtc ggcacgcttt cgggcgccgg gcggcgcgcg
     1801 ccgatgagga agtcgcgcgc ataccaggcg aggcgtgcgt tctcgatgcg gtcgaaggcg
     1861 taggcgccct ggaacagcgg attcaccggg cggcggctgc gcgcgggctc gagcatctcg
     1921 agcaccggca cctcgagatt gagcagcgcg accaggtcgg tggcggagcg ctccgtgaag
     1981 cggtggcgcg ccgcgttcag gtcgagcagc ggcgcgtagt ccgagttggc cgccacgccg
     2041 tagctcgcga agagcggctc gagcaggccg cggccgccga ggtagcgcgc gtcgagatcg
     2101 ccggcgctga gcacgtgcac cgtccagagc tccttggcga ggcccgggtg ctcgaacacg
     2161 cgcgcctgcg gcggcagcgg cagctcgcgg ctgccggcga cgatcagcag gtcgtggtcg
     2221 ctcggcgcga acaccgcgta gtgcgggaac tcctcgccga gcgcccccat caccgaggcg
     2281 accagcgaag cgtcgatctc gtagagctgg aaccattgca ccagcaggcc gccgggctcg
     2341 aggtggccgc ggatgcggcg gtagaactcg cgcgtgaaca ggctcgccac gccgctcacc
     2401 caggggttgg agggctccga gatgatcagg tcgtagcgcg cgccgctcgc cgagaagaag
     2461 ctcttcgcgt cgtcgatcag gatgctgccg cgcgggtcgg caaaggcgcc gctgttgcgc
     2521 ggcatgaagc cgcgcgccgc ctcggccatc gccgcctcga tctcgacggt gtcgacgtgc
     2581 tcgatggcga ggctctgcag cagggtatgg gtggtgagcc cggtgccgat gccgatgacc
     2641 gccgcgcgcc gcgtgtcggg cttgagcgcg agcggcacgg cggcggtgag caccatcgtg
     2701 atctcgtcgc tgccgcgcgt caggtcgcgg tcgaggacga tcgagccgtc ggacttgccg
     2761 ttggtgcgca ggctggttgc gtcggcgtat tgcacgaggt gcaccgtggc ggtcttgccg
     2821 tccttcgcga acaggatcct ggcgtcgcgc gaggtggcga gctcgccgtg gcggaacacg
     2881 ccggaggtca tcttgtgcgg gtcgagctgc acgccggcgc cgatcgcgac gaggatcgca
     2941 gccgatgcgg ctgccgccca gatgaactgc ctgtgcgaga agaaattcag cagaaccagg
     3001 cccaggaccg cgtccaccag gcagccggcg agcagcgtga acttgaggcc gaggagcggc
     3061 aggccggcgt gcacggcgag caccacgccg gcgatcgcgc cgagcgtgtt cgcggcgtag
     3121 acccgcccga tcgcggcctc gccggcgccg cggcgcagca gcgcgccggc gagcagcggc
     3181 agcgtcatgc cggcgcagaa ggtggccgga agcatgacca gcgccgcgat cgccgcgccc
     3241 gacagattga acagcaggta gccggtgttg ttgcgcgcca gcccggccat cagcgcttcc
     3301 atcaggtcga aggtgaagtc gtagaccggc agcgtggctg ccgcggcgag cgccatcgcc
     3361 acctgcgccc agccgagcag ccgctcgggg gccggcgagg cgtcgacgcg tcgcctgatc
     3421 gccagaccgc cgagcgcgag cccggagatg aacgtggcca gcatcagttc gaaggcgtgc
     3481 gtcgaggcgc ccagcaccag cgacagcatg cggatccaga cgatctcgta gacgaacgag
     3541 gccaggccgg tgaagaacgc gactgctaga aggagggttg cgctttccgc tcgagaatca
     3601 cctctcacag gtgaaagatg aagcggcctg caaagcaacc agacca
//
LOCUS       DASYJA010000203         4330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1392661, whole genome shotgun sequence.
ACCESSION   DASYJA010000203 DASYJA010000000
VERSION     DASYJA010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392661"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1408)
                     /gene="ftsH"
                     /locus_tag="VGO02_04365"
     CDS             complement(<1..1408)
                     /gene="ftsH"
                     /locus_tag="VGO02_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV7476955.1"
                     /translation="MERRTRFNLVYLLFAMFAILSLQQWWQHAQTVEVVPYSDFERLL
                     AADKVSEVVVSDQRITGKLKAPENGKTVVVANMVAPDVAERLSKYGVKYTRASENNLF
                     RDILSWVVPALVFFGVWYLLVRRMSGQQGGMGGFMAIGKSRAKVYVESKTGVTFRDVA
                     GVDESKAELKEVVEFLRDPKKYGRLGARVPKGVLLVGPPGTGKTMLARAVAGEAGVPF
                     FSISGSEFVEMFVGVGAARVRDLFEQARQKAPAIIFIDELDALGRARGANPMGGHDEK
                     EQTLNQLLVELDGFDPSTGLVLLSATNRPEILDPALLRAGRFDRVVLVDRPDKKGRLE
                     ILQVHARKVKLGSDVDMEKLAAMTPGFSGADLANLINEAALLATRRGAEAVTQADFNE
                     AIERLIAGLEKKNRVLNETERRVVAHHELGHALVALALPGSDPVHKISIIPRGVAALG
                     YTLQRPTEDRFIMTREELE"
     gene            1508..4105
                     /gene="clpB"
                     /locus_tag="VGO02_04370"
     CDS             1508..4105
                     /gene="clpB"
                     /locus_tag="VGO02_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEV7476956.1"
                     /translation="MRFEKLTTKLQEALGEAQSLALGKDQQYIEPAHLLLALLNEEGG
                     GVAGLISKAGGNVSLLKKNAVNSIEKLPKVQGTPGEVMMGRELTQLLNVADKEAQKRG
                     DQYIASELFLLAAAGDKGSTGDLLKAAGVTKKSLEKAIEEIRGGESVSGQADESQRGA
                     LAKYTLDLTERARQGKLDPVIGRDEEIRRCIQILQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINAEVPESLKGKRLLVLDLASLLAGAKYRGEFEERLKSVLKELAKDEGSTIIF
                     IDEIHTLVGAGKAEGAIDAGNMLKPALARGELHCIGATTLDEYRKNIEKDAALERRFQ
                     KVLVEEPSVEATIAILRGLQERYEVHHGVEITDPAIVAAAELSQRYITDRFLPDKAID
                     LIDEAAARIKMEIDSKPEALDRLERRLIQLRIEREAVRKEKDEASQKRFSLIEEEIER
                     LEKDYSDLEEEWKAEKAAVQGTAHIKEAIEKLRQEMAELQRKGQYDRLAELQYGKLPQ
                     LENQLKQAEKKQPQTKPKLLRTQVGAEEIAEVVSRATGIPVAKMMTGEREKLIRMEER
                     LHERVVGQDEAVRLVSDAIRRSRSGLSDPNRPYGSFLFLGPTGVGKTELCKALAGFLF
                     DSEDRLIRIDMSEYMEKHSVSRLIGAPPGYVGYDEGGQLTEQVRRKPYSVILFDEVEK
                     AHPDVFNTLLQVLDEGRLTDGQGRTVDFKNTVLVMTSNLGSQMIQSMAGSEYSAIKAA
                     VMAEVKTQFRPEFINRIDEIVVFHALSEEHIKSIARVQLKVLQARLAKMDYELEVSDA
                     ALSELAKAGFDPVYGARPLKRAIQSELENPLAKAILEGRFAPKDAIYVDWRGGKMVFE
                     KRSTARRAA"
     gene            complement(4083..>4330)
                     /locus_tag="VGO02_04375"
     CDS             complement(4083..>4330)
                     /locus_tag="VGO02_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476957.1"
                     /translation="VMMYMMDNPHAKGMLMGWVPDARLGYVTDIWTPGPPLPAKPNPG
                     LMSVVNTVKAAGLQPERFAGGHGATADYASLTRLAGQ"
BASE COUNT          823 a         1397 c         1448 g          662 t
ORIGIN      
        1 tctccagctc ctcgcgcgtc atgatgaagc ggtcctcggt cgggcgctgc agcgtgtagc
       61 cgagcgcggc gacgccgcgc gggatgatcg agatcttgtg caccgggtcg ctgccgggca
      121 gggccagggc caccagcgcg tggccgagct cgtggtgcgc gaccacgcgc cgctcggtct
      181 cgttcaggac gcggttcttc ttttccagcc cggcgatcag gcgttcgatc gcctcgttga
      241 aatcggcctg ggtcaccgct tcggcgccgc ggcgcgtggc gagcagcgcc gcttcgttga
      301 tcaggttggc gaggtccgcg ccggaaaagc ccggcgtcat ggcggcgagc ttctccatgt
      361 cgacgtcgga tcccagcttc accttcctcg cgtgcacctg caggatctcg aggcggccct
      421 tcttgtcggg ccggtcgacc agcaccacgc ggtcgaagcg gccggcgcgc agcagggccg
      481 ggtcgaggat ctcggggcgg ttggtcgccg agagcagcac caggccggtg ctcgggtcga
      541 agccgtcgag ctccaccagc agctggttca gcgtctgctc cttctcgtcg tggccgccca
      601 tcgggttcgc gccgcgcgcg cggccgagcg cgtccagctc gtcgatgaag atgatggccg
      661 gcgccttctg ccgcgcctgc tcgaacagat cccgcacgcg cgccgcgccc acgccgacga
      721 acatttcgac gaattccgat ccggagatcg agaagaaggg cacgccggcc tcgccggcca
      781 cggcccgcgc cagcatcgtc ttgccggttc cgggtggtcc gaccagcagc acgcccttcg
      841 gcacgcgcgc cccgagccgg ccgtatttct tcgggtccct gaggaactcg accacttcct
      901 tcagctcggc cttcgactcg tcgacgccgg ccacgtcgcg gaaggtgacg ccggtcttgc
      961 tctcgacgta gaccttggcg cggctcttgc cgatcgccat gaagccgccc atgccgccct
     1021 gctgccccga cattcgtcgc accagcaggt accagacgcc gaagaagacc agcgccggca
     1081 ccacccagga caggatgtcg cggaacaggt tgttttcgct cgctcgcgtg tacttgacgc
     1141 cgtacttcga caggcgctcg gcgacgtcgg gcgccaccat gttggcgacg accaccgtct
     1201 tgccgttctc cggcgccttc agcttgccgg tgatgcgctg gtcggagacc acgacctcgg
     1261 acaccttgtc ggcggcgagc agcctctcga agtcgctgta gggcaccacc tcgaccgtct
     1321 gcgcgtgctg ccaccactgc tgcagcgaga ggatggcgaa catggcgaac agcagataga
     1381 ccaggttgaa gcgcgtgcgt cgttccatga agtacctcat gaagcaataa ataggcttga
     1441 acgggccgaa ctcaacccca tctggcccta agggactatt cccgggttta cattacggat
     1501 caaggtcatg cgtttcgaga aactgacgac caagctgcag gaggcgctgg gcgaggccca
     1561 gagcctggcg ttggggaagg atcagcagta catcgagccc gcccacctgc tcctcgccct
     1621 gctcaacgag gagggcggcg gcgtcgccgg cctgatcagc aaggcggggg gcaatgtttc
     1681 gcttctcaag aagaatgccg tcaattcaat tgagaagctt ccgaaggtgc agggcacccc
     1741 cggcgaagtc atgatggggc gcgagctgac gcagctcctc aatgtcgccg acaaggaagc
     1801 gcagaagcgc ggcgaccagt acatcgcttc cgagttgttc ctgctcgctg ccgccggtga
     1861 caagggaagc acgggcgatc tcctgaaagc cgcaggggtg acgaaaaaat ccctcgaaaa
     1921 agccatcgag gagatcaggg gtggggaatc ggtgagcggt caggcggacg aatcgcaacg
     1981 cggggcgctc gcgaagtaca cactggatct aactgaaaga gcaaggcaag gaaagctcga
     2041 cccggtgatc gggcgcgacg aggagatccg ccgctgcatc cagatattgc aaagacgcac
     2101 gaagaacaac ccggtgctga tcggcgagcc cggcgtgggc aagaccgcca tcgtcgaggg
     2161 cctggcgcag cgcatcatca acgccgaggt gcccgagtcg ctgaagggga agcgcctgct
     2221 ggtgctcgac ctcgcctcgc tgctcgccgg cgccaagtac cgcggcgagt tcgaggagcg
     2281 gctgaaatcg gtattgaagg agctggcgaa ggacgagggc agcaccatca tcttcatcga
     2341 cgagatccac acgctggtcg gcgccggcaa ggccgagggc gcgatcgacg ccggcaacat
     2401 gctcaagccc gccctcgccc ggggcgagct gcactgcatc ggcgccacca cgctcgacga
     2461 gtaccgcaag aacatcgaga aggacgccgc cctcgagcgg cgcttccaga aggtgctggt
     2521 agaggagccg agcgtggagg cgaccatcgc catcctgcgc ggcctgcagg agcgctacga
     2581 ggtgcaccac ggcgtcgaga tcaccgaccc ggcgatcgtc gccgcggccg agctgtcgca
     2641 gcgctacatc accgaccgct tcctgcccga caaggcgatc gacctgatcg acgaggcggc
     2701 ggcgcgcatc aagatggaga tcgactccaa gcccgaggcg ctcgaccgcc tcgagcggcg
     2761 cctgatccag ctgaggatcg agcgcgaggc ggtgcgcaag gagaaggacg aggcttccca
     2821 gaaaagattt tccttgatcg aagaagaaat agaaagactg gagaaggact actccgacct
     2881 tgaggaagag tggaaggcgg agaaggcggc cgtccagggc accgcgcaca tcaaggaagc
     2941 gatcgagaag ctgcgccagg agatggccga gctgcagagg aagggccagt acgaccgctt
     3001 ggcggagttg cagtacggca agcttcctca attggaaaat cagcttaaac aggcagagaa
     3061 gaagcaaccc caaacaaaac ccaagctgct gcgcacgcag gtcggcgcgg aggagatcgc
     3121 cgaggtcgtg tcgcgcgcca ccggcatccc ggtagcgaag atgatgaccg gcgagcgcga
     3181 gaagctgatc aggatggaag agcgcctgca cgagcgtgtc gtcggccagg acgaggcggt
     3241 gcgcctcgtt tccgacgcca tccgccgctc gcgctcgggg ctgtccgacc cgaaccggcc
     3301 ctacggatca ttcctcttcc tggggcccac cggggtgggc aagaccgagc tctgcaaggc
     3361 gctcgccggc ttcctgttcg attccgaaga ccgcctgatc cgcatcgaca tgtccgagta
     3421 catggagaag cattcggtga gccgcctgat cggcgccccg ccgggctatg tcggctacga
     3481 cgagggcggc cagctcaccg agcaggtgcg ccgcaagccg tattcggtga tcctgttcga
     3541 cgaggtggag aaggcgcacc cggatgtctt caacacgctg ctgcaggtgc tggacgaagg
     3601 tcgcctgacc gacggccagg gccgcacggt cgacttcaag aacacggtgc tggtgatgac
     3661 ctccaacctc ggctcgcaga tgatccagag catggcgggc agcgaataca gcgccatcaa
     3721 ggccgcggtg atggccgagg tgaagacgca gttccggccg gagttcatca accgcatcga
     3781 cgagatcgtg gtgttccacg ccctgagcga ggagcacatc aagtcgatcg cgcgggtgca
     3841 gctcaaggtg ctgcaggcgc ggctggcgaa gatggactac gagctcgagg tttcggacgc
     3901 cgctctaagc gagctcgcca aggcgggctt cgacccggtg tacggcgcgc ggccgctcaa
     3961 gcgcgccatc cagtccgagc tcgagaaccc gctggcgaag gcgatcctcg aaggccgctt
     4021 cgcgccgaag gacgcgatct acgtcgattg gcgcggcggc aagatggtgt tcgagaagcg
     4081 ctctactgcc cggcgagccg cgtaagcgag gcgtagtccg cggtcgcgcc gtggccgccg
     4141 gcgaagcgct cgggctgcag cccggcggcc ttcacggtgt tcaccaccga catcaagccg
     4201 ggattcggct tggccggcag cggcggcccc ggcgtccaga tgtccgtcac gtagccgagc
     4261 ctcgcatcgg gcacccagcc catcagcatg cccttggcgt gcgggttgtc catcatgtac
     4321 atcatcacct
//
LOCUS       DASYJA010000204         1781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1030590, whole genome shotgun sequence.
ACCESSION   DASYJA010000204 DASYJA010000000
VERSION     DASYJA010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030590"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..842)
                     /locus_tag="VGO02_04380"
     CDS             complement(36..842)
                     /locus_tag="VGO02_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV7476958.1"
                     /translation="MKALFGDYPVTKLFKQQSTLEFADVKVPNTAFKRVVRELEFDVA
                     ELAIMTFLLARAHGTPYRLLPAVMTARFQHSTLAYNPGRGKLSPGQLAGKKVGMRSYS
                     VTTATWVRGILADDYGVDLDKVNWITFEDPHVAQFRDPPNATRMPAGKDAAAMLLAGE
                     IDAAILAEPPKDGPLKTLIPDPAAAAAAWKAKNRAIQINHMVTVKDTVSDSQAQDVFS
                     LLKKSREAAGNPEMNPFGLEENRRNLEVAIDCTYRQGMIPRKFAVEELFR"
     gene            complement(839..1528)
                     /locus_tag="VGO02_04385"
     CDS             complement(839..1528)
                     /locus_tag="VGO02_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HEV7476959.1"
                     /translation="MSGTVVRNIHRADAGAIEALGRQGVATVHEAQGRTGLMQPYMRP
                     VWRGARIAGSAVTALCHPGDNWMIHVACEVLKKGDILVVACSSENSDGGFGELLATSL
                     KALGAKGVILDIGCRDAAEISEMKFPVWSRAISAKGTVKASVGSVNVPVVCAGVNVKA
                     GDVIVADDDGVVVVDRKQAAEVARLGAEREKKEAGSRARLQKGELGLDIYDMRKTLAD
                     KGLKYVDGPLE"
     gene            complement(1530..>1781)
                     /locus_tag="VGO02_04390"
     CDS             complement(1530..>1781)
                     /locus_tag="VGO02_04390"
                     /inference="COORDINATES: protein motif:HMM:NF019366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallate dioxygenase"
                     /protein_id="HEV7476960.1"
                     /translation="LTDSSFRDAFKNDPEKAMKDLTEQEKDMVRRLDWKAMQDYGASF
                     FCLEKLARTMGVSNPQMVAAFRGETLEQFLKTRRVPGAR"
BASE COUNT          277 a          592 c          555 g          357 t
ORIGIN      
        1 cggcgcggtg gtgtagtggc cgtggcagtc gacgatcatc ggaacaactc ctccactgcg
       61 aatttgcgcg ggatcattcc ctgcctgtag gtgcagtcga tggcaacctc caggttccgc
      121 cgattctctt cgaggccgaa cggattcatt tcgggattcc ccgccgcttc cctgctcttt
      181 ttcaagagcg aaaagacatc ttgagcttga gaatcggaaa ccgtgtcttt cacggtcacc
      241 atgtgattga tctggatcgc acggttcttc gccttccatg ccgccgccgc ggcggccgga
      301 tcggggatca gcgtcttcag cggcccgtcc ttcggcggct cggcgaggat cgcggcgtcg
      361 atctcccccg caagcagcat cgctgcggcg tccttgcccg ccggcatgcg cgtggcattc
      421 ggcggatcgc ggaactgcgc gacgtgcggg tcctcgaagg tgatccagtt caccttgtcg
      481 aggtcgacgc cgtagtcgtc ggcgaggatg ccgcgcaccc aggtggcggt ggtgaccgaa
      541 taggagcgca tgcccacctt cttgccggcg agctggccgg gcgagagctt gccgcgcccg
      601 gggttgtagg cgagcgtgga gtgctggaag cgcgcggtca tcacggccgg cagcagtcgg
      661 tagggcgtgc cgtgggccct ggcgagcaga aaggtcatga tcgccagctc ggcgacgtcg
      721 aactccagct cgcgcaccac gcgcttgaac gcggtgttgg gcaccttgac gtcggcgaat
      781 tcaagcgtgg attgctgctt gaacagcttt gttacggggt agtccccgaa aagcgctttc
      841 actccagggg cccgtccacg tacttcaggc ccttgtccgc cagggtcttc ctcatgtcgt
      901 agatatccag gcccagctcg cccttctgca ggcgcgcgcg gctgccggct tccttcttct
      961 cgcgctcagc gcccaggcgc gcaacctccg ctgcctgttt tctatcaaca acaacaacgc
     1021 catcgtcgtc ggccacgatc acgtcgccgg ccttcacgtt tacgccggcg cacactaccg
     1081 gcacgttgac cgagccgacg ctcgccttga cggtgccctt ggcggagatg gcgcgcgacc
     1141 ataccgggaa tttcatctcg ctgatctcgg ccgcgtcgcg acagcctatg tcgaggatta
     1201 cgcccttcgc gccaagcgcc ttgagcgagg tggcgagcag ctcgccgaag ccgccgtcgc
     1261 tgttctcgct ggagcaggcc acgacgagaa tgtcgccttt cttcagcacc tcgcaggcga
     1321 catggatcat ccagttgtcg ccggggtggc agagcgcagt aaccgcgctg ccggcgatgc
     1381 gcgcgccgcg ccagacgggc cgcatataag gctgcatcag gccggtgcgc ccttgcgctt
     1441 cgtgcaccgt ggccacgccc tggcgcccga gggcctcgat cgcgccggca tcggcgcggt
     1501 ggatgtttcg tacgacggtt ccgctcatgt catcgtgctc cggggaccct gcgggttttc
     1561 aggaactgct cgagcgtctc gccgcggaac gccgccacca tctgcgggtt cgacacgccc
     1621 atcgtgcgcg ccagcttctc caggcagaaa aagctcgcgc cgtagtcctg catcgccttc
     1681 cagtccaggc gccgcaccat gtccttttcc tgctcggtca agtctttcat tgctttctca
     1741 ggatcgttct tgaaagcgtc cctgaaagaa gaatcggtca a
//
LOCUS       DASYJA010000205         6024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1938042, whole genome shotgun sequence.
ACCESSION   DASYJA010000205 DASYJA010000000
VERSION     DASYJA010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1938042"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VGO02_04395"
     CDS             <1..217
                     /locus_tag="VGO02_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476961.1"
                     /translation="LEPWSFGGEKGFRFEFTFTRKVDGVLNSGVGYGTVSKGELFTLL
                     YAAPRLGFFPRHKDKVEQIARSARLKA"
     gene            complement(207..905)
                     /gene="queC"
                     /locus_tag="VGO02_04400"
     CDS             complement(207..905)
                     /gene="queC"
                     /locus_tag="VGO02_04400"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEV7476962.1"
                     /translation="MSAGALVLFSGGQDSTTCVAWALERYARVETIGFDYRQRHRIEL
                     DARQTILSRLRREFPHWDARLGADQVIDLGVLAQIGESALTREIQIEMGTNGLPNTFV
                     PGRNLLFLTFAAALAYRRGIDVLVGGMCETDFSGYPDCRDATMKAMQSALTLGLDRGV
                     TIETPLMWIDKAATWQMAKDLGGDKLIALIREDSVTCYLGERGSRHAWGHGCGACPAC
                     ELRGRGYEKWLARP"
     gene            complement(908..1669)
                     /locus_tag="VGO02_04405"
     CDS             complement(908..1669)
                     /locus_tag="VGO02_04405"
                     /inference="COORDINATES: protein motif:HMM:NF023984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HEV7476963.1"
                     /translation="MKFLKRVGYALMAGLLLGGGVAVTAQDWRTASREPTGVAPDPKL
                     VREAVVQVYGARTVGVKGYFGVHTWVAVKPTDAPDWTVYEVIGWRLRYQDTALVVRNR
                     APDGRWFGAEPELYADRRGAGVDELIKRIDKAARSYPYAGEYSVWPGPNSNTFTSWIA
                     RAVPELEIDLPPTAIGKDYLRGAVVASAPSGSGYQISLRGLLGVAASSVEGLELNLLG
                     LSFGLGPHGLRLPFVGTLGPARSFAAAPAPAAHQP"
     gene            complement(1721..2545)
                     /gene="panB"
                     /locus_tag="VGO02_04410"
     CDS             complement(1721..2545)
                     /gene="panB"
                     /locus_tag="VGO02_04410"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEV7476964.1"
                     /translation="MKPTVLELRGKKGQRLAMLTAYDFPTARLAAEGGIDLILVGDSL
                     GMVVLGYDSTLPVTLDDMVHHTKAARRGAPEAFLIADLPFLSYATPEQALASAARLMK
                     DAGADSVKLEGGEEAVPAVEALVRAGVPVLGHVGLTPQTASSLGGYKLQGKDEETARR
                     ALDGAVALERAGCWGVVLELVPAPLAKLVTERLAIPTIGIGAGADCDGQVLVFHDLVG
                     MFSGFTPTFVKRYTEAGAAIRAAISRYAAEVRSGAFPGEAQSFRMKDEVLRRIYGG"
     gene            complement(2542..3381)
                     /gene="ttcA"
                     /locus_tag="VGO02_04415"
     CDS             complement(2542..3381)
                     /gene="ttcA"
                     /locus_tag="VGO02_04415"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEV7476965.1"
                     /translation="MTAPRKAHYEENKLAKRLRHQVGDAISDFNMIEAGDKVMVCLSG
                     GKDSHSLLDVLLSLQEKSPVKFELIAVNLDQKQPGFPARVLPEYLESRGVPFHIEEQD
                     TYSTVKRVIPIGKTMCSLCSRLRRGILYRVAGELGATKIALGHHRDDVLATFLLNLFH
                     GGKLKAMPAKLVSDDGRHTVIRPLVYVSEADLVEFSKIKTFPIIPCTLCGSQDNLQRQ
                     VVGEMLRGWEKAHPGRIESILRALTDVVPSHLLDRKLFDFANLKPATAGEAQEGEPLF
                     AEA"
     gene            complement(3389..4135)
                     /locus_tag="VGO02_04420"
     CDS             complement(3389..4135)
                     /locus_tag="VGO02_04420"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF006598.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HEV7476966.1"
                     /translation="MDDGLQGRTALVTGGARRVGAAIVRRLHAAGANVVLHYRGAEAE
                     AAKLEAELNAARAKSAARVKAELLAPIAPRALVSAALESFGRLDLLVNNASSFFPVAV
                     GAIEASHWEELVGSNLKAPLFISQEAAPHLGKHQGAIVNIVDIHAERPLKGYPLYSIA
                     KAGLAALTRSLAVELAPAVRVNGVAPGAIAWPEDGQFADAERGRIVATTPLGRVGSPE
                     EIAQAVHFLACAPYVTGQILAVDGGRSIYH"
     gene            4168..5211
                     /locus_tag="VGO02_04425"
     CDS             4168..5211
                     /locus_tag="VGO02_04425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEV7476967.1"
                     /translation="MISRIAGEIAAHGGWIPFARYMELALHEPGLGYYAGGARKFGAE
                     GDFVTAPELGGLFGRTLARQLREFPAILEIGAGSGALAETLLQELDCEYLILETSTEL
                     RDRQQRRLGGKVRFLDTLPERFSGAIVANEVVDAMPVHAVAWRADGIMERGVAFENHE
                     LVWREKPATGGLRNEAQKIELPIPYESEIGLAGRAWLRTLGERLESGAILVIDYGFPR
                     AEYYHPQRSMGTLMCHHRHRSHDDVFFEPGGQDITAHVDFSALAEAARGAGLDVLGYA
                     TQAQFLVNCGITDVLAEANAANALHYAPIAAEAQQLLSPAEMGELFKVLAVGRGVKHP
                     LLGFSRNDRSHTL"
     gene            5214..5864
                     /locus_tag="VGO02_04430"
     CDS             5214..5864
                     /locus_tag="VGO02_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476968.1"
                     /translation="MSRLGLLLLLAGFAAASETEEAKKRWEASPQGPMLERILPPTFE
                     ARQLPEPGGQGAGLAQRYCVQCHNLPNPAMHHAAKWPAIVDRMVVRMEGHGNLGKVMV
                     EMMAGVKAPAPEETQVLIQYLQKHSQTPLDEKRYPEVNRPSGEAFRLACNQCHVLPDP
                     QRHTAAEWRAVVARMQENMEWMNRVVGTQPASDRAAGEPQLRVEEINAFLARYARP"
BASE COUNT          983 a         2048 c         2063 g          930 t
ORIGIN      
        1 gctcgagccg tggagcttcg gcggcgaaaa aggcttccgc ttcgagttca cgttcactcg
       61 caaggtcgac ggggtgctca actccggcgt cggctacggc acggtgagca aaggcgagct
      121 gttcacgctg ctctacgcgg cgccgcggct cggcttcttt ccgcgccaca aggacaaggt
      181 ggagcagatc gcgcgcagcg cgcgcctcaa ggcctagcca gccatttttc atagccgcgg
      241 ccgcgcagtt cgcaggccgg gcaagcgccg cagccgtggc cccaggcgtg ccgcgagccg
      301 cgctcgccga ggtagcaggt cacgctgtct tcgcggatca gcgcgatcag tttgtcgccg
      361 ccgaggtcct tcgccatctg ccaggtggcg gccttgtcga tccacatgag cggcgtctcg
      421 atggttacgc cgcgatcgag cccgagcgtg agcgctgact gcatcgcctt catcgtggcg
      481 tcgcggcagt ctggatagcc ggagaagtcg gtctcgcaca tgccgcccac caggacgtcg
      541 atgccgcgcc ggtacgccag ggcggcggcg aaggtgagaa acagcagatt gcgcccgggc
      601 acgaaggtgt tgggcaggcc gttcgttccc atctcgatct ggatctcgcg ggtcagggcg
      661 ctttcgccga tctgcgccag cacgccgagg tcgatgacct ggtcggcgcc gaggcgcgca
      721 tcccagtgcg ggaactcgcg ccgcaggcgc gaaaggatcg tctggcgggc gtcgagctcg
      781 atgcgatggc gctggcgata atcgaagccg atggtctcga cgcgcgcgta gcgctcgagc
      841 gcccaggcca cgcaggtcgt cgaatcctgt cccccggaaa agaggaccag cgcgccagcg
      901 gacagagcta gggctggtga gccgcgggcg cgggcgcggc ggcgaacgag cgcgccggac
      961 cgagcgtgcc gacgaacggc agcctgagac cgtgggggcc gagtccgaag ctgagcccga
     1021 gcagattcaa ctcgaggcct tcgacggagc tcgcggcgac accgagcagc cccctgaggg
     1081 agatctggta gccgctgccg ctgggcgcgc tggcgaccac ggcgcctcgc aggtagtcct
     1141 tgccgatcgc cgtcggcggc agatcgattt ccagctccgg cacggcgcgc gcgatccagg
     1201 aagtgaacgt attcgagttc ggccccggcc agacgctgta ttcgccggcg taggggtagc
     1261 tgcgcgccgc cttgtcgatg cgcttgatca gctcgtcgac gcccgcgccg cgcctgtcgg
     1321 cgtagagctc gggctcggcg ccgaaccagc ggccgtccgg cgcgcggttg cgcaccacca
     1381 gcgccgtgtc ctggtagcgc aggcgccagc cgatcacttc gtagaccgtc cagtccgggg
     1441 cgtcggtcgg cttgaccgcc acccaggtgt ggacgccgaa gtaacccttc acgcccaccg
     1501 tacgggcgcc atagacctgc accaccgctt cgcgcaccaa tttgggatcc ggcgcaacac
     1561 cggtcggctc gcggctcgcg gtgcgccaat cctgcgccgt caccgccacg ccgccgccca
     1621 gcaacaaccc cgccatgagg gcatacccaa cccgcttgag gaatttcatc agagttcttg
     1681 caccttcgac ttcaacgccg ggcgatggtt cgcagttgac ctagccgcca tagatccgcc
     1741 tcaggacctc gtccttcatg cggaagcttt gcgcctcgcc cgggaaggcg cccgagcgca
     1801 cctcggcggc atagcgggaa attgcagcgc ggatcgcggc gcctgcctcg gtgtagcgct
     1861 tgacgaaagt cggcgtgaag ccggagaaca tgccgaccag gtcgtggaac accagcacct
     1921 ggccgtcgca gtcggcgccc gcgccgatgc cgatggtcgg aatggcgagg cgctcggtga
     1981 ccagcttggc gaggggcgcc ggcacgagct cgagcaccac cccccagcag ccggcgcgct
     2041 ccagcgcgac cgcgccatcg agggcgcggc gtgcagtctc ttcgtccttg ccctgcagct
     2101 tgtagccgcc gagcgaggac gcggtctgcg gcgtgaggcc gacgtggcca agcaccggca
     2161 ccccggcgcg caccagcgcc tcgacggcgg gaacggcttc ctcgccgccc tcgagcttca
     2221 ccgagtcggc gccggcatcc ttcatcaggc gcgcggcgct ggcgagcgcc tgctccggcg
     2281 tggcatagga aaggaacggc aggtcggcga tcaggaaggc ctcgggcgcg ccgcggcgcg
     2341 cggccttggt gtgatggacc atgtcgtcga gcgtcaccgg cagcgtcgag tcgtagccga
     2401 gcaccaccat gccgagcgag tcgcccacca ggatcaggtc gatgccgccc tcggccgcca
     2461 ggcgcgcagt cgggaaatcg taggcggtga gcatggcgag acgctggccc ttcttgccgc
     2521 gaagctcgag aacggtaggc ttcatgcttc agcgaaaagg ggttcgccct cctgcgcttc
     2581 gcccgcggtg gcgggtttca aattggcgaa gtcgaacagc ttgcggtcga gcaggtgcga
     2641 gggcacgacg tcggtgagcg cgcgcaggat cgactcgatg cgtccgggat gcgccttctc
     2701 ccagccgcgc agcatctcgc cgaccacctg gcgctgcagg ttgtcctgcg agccgcacag
     2761 cgtgcagggg atgatcggaa acgtctttat tttcgagaac tcgaccagat cagcctcaga
     2821 gacataaacc agcggccgga tcaccgtgtg gcggccgtcg tccgacacca gcttggccgg
     2881 catcgccttc agcttgccgc cgtggaacag gttgagcaag aacgtcgcca gcacgtcgtc
     2941 gcgatggtgg ccgagcgcga tcttcgtggc gccgagctcg ccggcgacgc ggtagaggat
     3001 cccgcggcgc agcctcgagc acagcgagca catggtcttg ccgatcggga tgacgcgctt
     3061 caccgtcgag tacgtgtcct gctcctcgat gtggaacggc acgccgcgcg actcgaggta
     3121 ctccggcagc acgcgcgccg ggaagccggg ctgcttctgg tcgaggttga cggcaatcaa
     3181 ttcaaacttc accggggact tctcttgcaa agacaacaga acatccagca ggctgtgact
     3241 atccttgccc cccgacaggc agaccatcac cttgtcgcct gcctcgatca tgttgaagtc
     3301 ggagatcgcg tcgcccacct ggtggcgcag gcgcttggcg agcttgttct cctcgtagtg
     3361 cgcttttctc ggggcggtca tgcggccttc agtggtagat cgagcgcccg ccgtccaccg
     3421 ccaggatctg ccccgtgaca tagggcgcgc aggcgaggaa gtgtaccgcc tgcgcgatct
     3481 cctcgggcga gccgacgcgc cccagcggtg tcgtggcgac gatacgcccg cgctcggcgt
     3541 cggcgaactg gccgtcctcg ggccaggcga tggcgcccgg cgcgacgccg ttgacgcgca
     3601 ccgccggcgc cagctcgacg gcgagcgagc gggtgagcgc ggccagcccg gccttggcga
     3661 tgctgtacag cggatagcct ttgagcggcc gctcggcgtg gatgtcgacg atgttgacga
     3721 tcgcgccctg gtgtttgccc aggtgcggcg ccgcttcctg cgagatgaag agcggcgcct
     3781 tcaggttcga gccgaccagc tcttcccagt gcgaggcctc gatcgcgccg acggcgacgg
     3841 ggaagaagct cgacgcattg ttcaccagca ggtcgaggcg gccgaagctc tcgagcgccg
     3901 ccgagaccag ggcgcgcggc gcgatcggcg ccagcagctc ggccttcacg cgcgcggcgc
     3961 tcttcgcgcg cgccgcattc agctcggctt cgagtttcgc agcctcggct tccgcgccgc
     4021 gatagtgcag caccacgttc gcgcccgccg cgtgcaggcg gcgcacgatc gccgcgccga
     4081 cgcggcgcgc gccgccggtc accaatgccg tcctgccttg cagcccgtcg tccatgaagg
     4141 tcttgaattc tagggtaaat tccgcgggtg atctcccgaa tagcaggcga aatcgcggcg
     4201 cacggcggct ggatcccctt cgcgcgctac atggagctcg cgctgcacga gcctggtctg
     4261 ggctactacg ccggcggcgc gcgcaagttc ggcgccgaag gcgacttcgt caccgcaccc
     4321 gagctgggcg gcctcttcgg ccgcacgctg gcccggcagc tgcgggaatt tccagcaatc
     4381 ctggagatcg gcgcgggcag cggcgcgctg gccgagaccc tgctgcagga actggattgc
     4441 gaatatctca tcctggaaac cagcaccgag ctgcgcgatc gccagcaaag gcggctcggc
     4501 ggcaaggtgc ggtttctcga cacgctgccc gagcgcttca gcggcgcgat cgtcgccaac
     4561 gaggtcgtcg atgccatgcc ggtgcacgca gtcgcgtggc gcgccgatgg aatcatggaa
     4621 cggggtgtcg cctttgaaaa ccacgaattg gtatggcgag aaaaaccggc gaccggaggg
     4681 ctgcggaacg aggcgcagaa aatagaacta ccgatcccgt atgagagtga gatcgggctc
     4741 gcgggccgcg cgtggctgcg cacacttggg gagaggctcg agtcgggcgc gatcctcgtc
     4801 atcgactacg gcttcccgcg cgcggagtac taccacccgc agcgctcgat gggcacgctg
     4861 atgtgccacc accgccatcg ctcgcatgac gacgtgtttt tcgagccggg cggccaggac
     4921 atcacggcgc acgtcgattt cagcgcgctc gctgaggcag cgcgcggggc gggcctcgat
     4981 gtgctcggct acgccacgca ggcccagttc ctggtgaact gcggcatcac cgacgtcctc
     5041 gccgaagcca atgccgccaa cgccctgcac tatgcgccga tcgccgccga ggcgcagcag
     5101 ctgctttccc ccgcggagat gggcgagctc ttcaaggtgc tcgccgtagg ccgtggcgtg
     5161 aaacacccgc tgctcgggtt ttcgcgtaac gaccgttcgc ataccctcta ggcatgtcca
     5221 ggctcggatt gcttctgctg ctggccggct tcgctgccgc cagcgaaacc gaagaagcga
     5281 agaaacgctg ggaagcttcc ccgcagggac cgatgctcga gcggatcctg ccgccgacgt
     5341 tcgaagcgcg ccagctgccc gagccgggcg ggcagggcgc gggccttgcc caacgctact
     5401 gcgtgcagtg ccacaacctg cccaaccctg ccatgcacca cgccgccaag tggccggcga
     5461 tcgtcgaccg catggtggtg cgcatggagg gccacggcaa cctgggcaag gtgatggtgg
     5521 agatgatggc cggggtcaag gcacctgcgc cggaagaaac gcaggtgctg atccagtatc
     5581 tgcaaaagca ctcgcagacg ccgctcgacg agaagcgcta ccccgaagtg aaccggccct
     5641 cgggcgaggc cttccgcctc gcctgcaacc agtgccatgt gctgcccgac ccgcagcgcc
     5701 atacggcggc cgaatggcgc gcggtggtgg cgcgcatgca ggagaacatg gagtggatga
     5761 accgggtggt cggcacgcag cccgcatctg accgggctgc cggcgagccg cagctgagag
     5821 tggaagagat caacgccttc ctcgcccggt acgcaagacc atgacgcaag gcactcctct
     5881 gcgccgcagg ctcctgctgc tcgtggcggc cgcgatcctg ccgctcgccg cgatggccgg
     5941 cctcggtctg ctcgcgctct tcgagcagca gtgcaaacag gcggagcgaa tcggcatcga
     6001 gctggcgcgc gttctggtca tcgc
//
LOCUS       DASYJA010000206         3181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_667410, whole genome shotgun sequence.
ACCESSION   DASYJA010000206 DASYJA010000000
VERSION     DASYJA010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667410"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VGO02_04435"
     CDS             complement(<1..642)
                     /locus_tag="VGO02_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012367024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7476969.1"
                     /translation="MIFRQLFDQQSSTYTYLLADEASREAVLIDPVYEQARRDAALIE
                     ELGLKLAWTLETHVHADHVTGAWLLKDRLGSRIAVAKAGGAEGSDLLLKDLDSIRFGK
                     RHLEAWATPGHTGGCLTYVLDDRMAAFTGDALLVRGCGRTDFQQGDARTLFRSVRSRI
                     FALPDACLVYPGHDYRGLAATSVGEEKLYNPRLGEAIGEQDFVGYMKNLGLAHP"
     gene            741..815
                     /locus_tag="VGO02_04440"
     tRNA            741..815
                     /locus_tag="VGO02_04440"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:773..775,aa:Val,seq:cac)
     gene            complement(850..1164)
                     /locus_tag="VGO02_04445"
     CDS             complement(850..1164)
                     /locus_tag="VGO02_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476970.1"
                     /translation="MSIKHPNLPFTAPLIAAAPETSGVFALWQNGAVVYYGKASSGNA
                     TIRSALDGHLKGRSFSSEKPTRCSWEVADNPELRHAQLLIEFENAHQRMPRWNDPERL
                     PT"
     gene            1251..1544
                     /locus_tag="VGO02_04450"
     CDS             1251..1544
                     /locus_tag="VGO02_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476971.1"
                     /translation="MGFAVLPYLNKYETAEASMPIKPNGNLCSIREQIVEDVATGLIL
                     QFECVEGRARLVIAGEKLPFGNREILFDDDGRESAAGTLVGQFRRPSWLKTVG"
     gene            complement(1551..>3181)
                     /locus_tag="VGO02_04455"
     CDS             complement(1551..>3181)
                     /locus_tag="VGO02_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV7476972.1"
                     /translation="ASFSRFSDIHQSFLDQLTESIGVVLHTISANMQTEYLLHQSQSL
                     TAELQSQQEELKKTNDRLETQALNLTKSETLLKGKQDELRTANEQLQEKARQLSEQMH
                     QVEFKNREIEQARAALEEKAEQLSLSSRYKSEFLANMSHELRTPLNSLLILARLLSDN
                     VGGNLTHKQVEFARTIHSSGAELLALINDILDLAKIESGTVTLNIAQEPFSDLRDYVE
                     RTFRQMAADKGLAFAVEIADALPESLGTDGKRLQQILANLLSNAFKFTSEGGVTLRLE
                     PAESGWTPGHPVLGGGEDGGVVAFSVIDTGIGIPAAKQRLIFEAFQQADGTTSREYGG
                     TGLGLSISRELARLLGGEIRVSSQPDEGSTFTLYLPLDYRPEMLDTVAPATPRLAAAE
                     RPSEPREVWPEAAPAEARPPQAQADGGIAGRKVLVVDDDIRNVFALASALEMHGLNVV
                     HADSGKEGIEILKRSQDIDLVLMDVMMPGMDGFDTMRLIRQLDGYGSLPIIAVTAKAM
                     LGDREKCIDAGATDYVAKPVNIDSLLGMLERSLS"
BASE COUNT          496 a         1024 c         1068 g          593 t
ORIGIN      
        1 cggatgcgcc aggccgaggt tcttcatgta gccgacgaag tcctgctcgc cgatcgcctc
       61 gcccaggcgc gggttgtaga gcttctcctc gccgacgctg gtggcggcga ggccgcggta
      121 gtcgtggccg gggtagacca ggcaggcgtc gggcagcgcg aagatgcggc tgcgcaccga
      181 gcggaacagc gtgcgcgcgt caccctgctg gaagtcggtg cggccgcagc cgcgcacgag
      241 cagcgcgtcg ccggtgaagg ccgccatgcg gtcgtcgagc acgtaggtca ggcagccgcc
      301 ggtatggccg ggcgtggccc aggcttcgag atgccttttc ccgaaacgaa tcgaatccag
      361 atcctttaaa agcagatccg atccttcggc gccgcccgcc ttggccacgg cgatcctgct
      421 gcccagccgg tccttgagga gccacgcgcc ggtgacgtgg tcggcgtgca cgtgcgtctc
      481 gagcgtccag gcgagcttga gccccagctc ctcgatgagc gccgcatcgc gccgcgcctg
      541 ctcgtacacc gggtcgatga gcaccgcttc ccgcgacgcc tcgtcggcga gaagatatgt
      601 atacgtggag gactgctgat cgaaaagctg tcggaagatc atttcttgtg aataactcta
      661 caccgctatc ggcgcgatga tcgccggtgg cctatttgcg ccggtggcct atttgctaga
      721 atcctgccct tcaaaggccc gggtgcttag ctcagcggta gagcactacc ttcacacggt
      781 aggggtcaca ggttcgatcc ctgtagcacc caccagaata aatggggtca gaccccattt
      841 attttttccc taggtcggca atctctccgg atcgttccag cgcggcatgc gctggtgcgc
      901 gttctcgaat tcgatcagca gctgcgcgtg gcgcagctcc ggattgtcgg cgacctccca
      961 gctgcagcgc gtcggcttct cgctgctgaa gctgcgtccc ttcaggtggc cgtcgagcgc
     1021 gctgcggatg gtcgcattgc cgctcgaggc cttgccgtaa tagacgacgg cgccgttctg
     1081 ccaaagcgcg aacacgcccg aagtttcggg cgctgccgcg atcagcggcg cggtgaaagg
     1141 taaattcggg tgcttgatgg acatggctcg ctcccccgct tttgaagatg caatgctacg
     1201 cccgcagcgg tacggccaca cctgtcggat aaacgccaca aaatcttggc atgggctttg
     1261 ctgtcctccc ctatctcaac aaatacgaaa cggcggaggc ttccatgcct atcaagccaa
     1321 acggaaattt atgctccatc cgtgaacaga tcgttgaaga cgtcgcaaca ggactgatcc
     1381 tgcagttcga atgcgtcgag ggacgcgcgc gcctggtgat cgccggcgag aagctgccgt
     1441 tcggcaaccg cgagatcctg ttcgacgacg acggccgcga atcggccgcg ggcacgctgg
     1501 tcggccagtt ccgccggccg tcgtggctca agacggtcgg ctaaggcgct ctagctcagc
     1561 gagcgctcga gcatccccag cagcgaatcg atgttgacgg gcttggcgac gtagtcggtc
     1621 gcgccggcat cgatgcattt ctcgcggtcg ccgagcatcg ccttggcggt caccgcgatg
     1681 atgggaagcg agccgtagcc gtcgagctgc cggatcaggc gcatggtgtc gaagccgtcc
     1741 atgcccggca tcatcacgtc catcagcacc aggtcgatgt cctggctgcg cttgaggatc
     1801 tcgatgcctt ccttgccgct gtcggcgtgc acgacgttca ggccgtgcat ctcgagcgcg
     1861 ctcgccagcg cgaagacgtt gcggatgtcg tcgtcgacca ccagcacctt gcggccggcg
     1921 atgccgccgt ccgcctgcgc ctgcggcggc cgcgcctcgg ccggcgccgc ctccggccac
     1981 acctcgcgcg gctcgctcgg ccgctcggcc gcggcgaggc gcggcgtcgc gggcgccacg
     2041 gtgtcgagca tctcggggcg gtagtcgagc ggcaggtaga gcgtgaaggt gctgccctcg
     2101 tcgggctggc tcgagacgcg gatctcgccg cccagcaggc gcgccagctc gcggctgatc
     2161 gacaggccca ggcccgtgcc gccgtactcg cggctggtgg tgccgtcggc ctgctggaaa
     2221 gcctcgaaga tcaggcgctg cttggccgcg gggatgccga tgccggtgtc gatcacggag
     2281 aacgccacca cgccgccgtc ttcgccgccg ccgagcaccg ggtggccggg cgtccagccc
     2341 gactccgccg gctcgaggcg cagggtgacg ccgccctcgc tggtgaactt gaaggcgttc
     2401 gacagcaggt tggctaggat ctgctgcagg cgcttgccgt cggtgccgag cgactcgggc
     2461 agcgcgtcgg cgatctcgac cgcgaaggcg agccccttgt cggccgccat ctggcggaag
     2521 gtgcgctcga cgtagtcgcg caggtcgctg aacggctcct gcgcgatgtt gagggtcacc
     2581 gtgcccgatt cgatcttcgc caggtcgagg atgtcgttga tcagcgcgag cagctccgcg
     2641 cccgaggaat ggatggtgcg cgcgaactcg acctgcttgt gggtcaggtt gccgccgacg
     2701 ttgtcggaca ggaggcgggc aaggattaga agcgaattga gcggcgtgcg cagctcgtgc
     2761 gacatgttcg cgaggaactc ggacttgtag cgcgagctga gcgagagctg ctccgccttc
     2821 tcctccagcg cggcgcgcgc ctgctcgatc tcgcggttct tgaactcgac ctggtgcatc
     2881 tgctcggaca gctggcgcgc tttctcctgc agctgctcgt tggcggtgcg cagctcgtcc
     2941 tgcttgccct tgagcagcgt ctccgacttg gtcaggttga gggcctgggt ctccaggcgg
     3001 tcgttggtct tcttcagctc ctcctgctgg ctttgcaact ctgcagtcag cgattgggac
     3061 tggtgcagca gatattccgt ctgcatgttc gccgagatgg tgtgcagcac gacgccgatc
     3121 gactcggtca gctggtcgag gaagctctgg tggatatcgc tgaacctcga gaacgaggcc
     3181 a
//
LOCUS       DASYJA010000207         3065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1030979, whole genome shotgun sequence.
ACCESSION   DASYJA010000207 DASYJA010000000
VERSION     DASYJA010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030979"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VGO02_04460"
     CDS             complement(<1..315)
                     /locus_tag="VGO02_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7476973.1"
                     /translation="MKVIVLGAGVIGVTGAWYLAEAGHDVTVVERRREAGMETSFANG
                     GQISAGHAEPWAQPAVLPKVLRWLGREDAPLLFRPRADWAQWEWGARFALECLPGRFE
                     RNC"
     gene            333..860
                     /locus_tag="VGO02_04465"
     CDS             333..860
                     /locus_tag="VGO02_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEV7476974.1"
                     /translation="MPLYSIGERRVALHGTHHYIAPDASIIGSVTLENDVSVWFNVVI
                     RADNDQVTIGEATNVQDGSVLHVDPGYPMTLGRRVTIGHKVMLHGCTIGDGALVGINS
                     VVMNGAKIGKSALIGANTLIAEGKEIPDGVLVLGSPGKIVRELKQEEKDYLLKIAAGY
                     VERSRFYKANLKEIT"
     gene            complement(853..1614)
                     /locus_tag="VGO02_04470"
     CDS             complement(853..1614)
                     /locus_tag="VGO02_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003273512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEV7476975.1"
                     /translation="MQIKNSVFLVTGGASGLGAATARMAAEGGGKVVIADLQADAGEK
                     LAKELGGRFAKTDVSSEADGKAAVAAALKEFGGLHVLVNCAGIALAERTIGKDAPHDL
                     GRFIKVLTVNVVGTFNMIRLAADAMAKAGPNASGERGVIVNTASVAAYDGQIGQAAYS
                     ASKGGIVGMTLPIARDLSRNGIRVNTIAPGIFETPLLAALPKEAQESLGKQVPFPSRL
                     GRAAEFAQLVKSIIEIEMLNAETIRLDGAIRMAPR"
     gene            1679..>3065
                     /locus_tag="VGO02_04475"
     CDS             1679..>3065
                     /locus_tag="VGO02_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476976.1"
                     /translation="MQLAFGIAFADLYDRAGLLRVDAAFLAFLREANDGLHARLLSAR
                     GNFAQLKPREESDLLVELAPHVEDFIARLFGIEAEARALAERHNELAPLYSVKRLFVQ
                     RHAMHKVKPEDAKPDGFAFTTELDFARQVTAWQQEAAANAGKLEAAARYAAWAASTPE
                     GKRKHRGGVLFKAPSKLDFMKLVPVRTETARGFAEHTLDHLRLRDGFKLTDPGTDLTG
                     ALDEANYCIWCHEQGKDSCSKGLKEKAPAESFKKTVFGVPLAGCPLEEKISEFHMVKV
                     RGQPLAALGIIAVDNPMVAATGHRICNDCMKSCIYQKQEPVNIPQAETRTLKDILELP
                     WGFEIYSLFTRWNPFDLHRPYPKPHSGRRVLVVGLGPAGFTLAHHLMNDGHTVVGIDG
                     LKIEPLDAAVSGVDALGHRVAFRPIRDIAELREPLDGRVMAGFGGVAEYGITVRWDKN
                     FLKLIRLLLERR"
BASE COUNT          533 a         1035 c          998 g          499 t
ORIGIN      
        1 gcaattgcgc tcgaagcggc cgggcaggca ttccagcgcg aagcgcgcgc cccattccca
       61 ctgcgcccag tcggcgcgcg gccggaaaag cagcggcgcg tcctcgcgtc cgagccagcg
      121 cagcaccttg ggcagcacgg ccggctgcgc ccacggctcg gcatggccgg cggagatctg
      181 gccgccgttg gcgaacgagg tttccatgcc ggcttcgcgc cggcgctcga cgaccgtcac
      241 gtcgtggccg gcctcggcga gataccaggc gccggtgacg ccgatcacgc cggctccgag
      301 gacgatgact ttcactgcat tatgatagcc gcatgcctct ttactcgatc ggtgagcggc
      361 gcgtcgcgct gcatggaacg catcactaca tagcgcccga cgccagcatc atcggctcgg
      421 tgacgctcga gaacgacgtc agcgtctggt tcaacgtggt gatccgcgcg gacaacgacc
      481 aagtgacgat cggcgaagcc accaacgtgc aggacggctc ggtgctgcac gtcgatcccg
      541 gctatccgat gacgctcggc cgacgcgtca ccatcggtca caaggtgatg ctgcacggct
      601 gcaccatcgg cgacggcgcg ctggtcggca tcaacagcgt ggtcatgaac ggcgcgaaga
      661 tcggcaaatc cgctctcatc ggcgccaata ccctgatcgc tgaaggcaag gagatccccg
      721 acggggtgct ggtgctcggc tcccccggca agatcgtgcg cgagctgaag caggaggaga
      781 aggactacct cctgaaaatt gccgccggct acgtcgagcg ctcccgcttc tacaaggcca
      841 acctgaagga aatcacctag gcgccatgcg gatcgcgccg tcgaggcgga tcgtctcggc
      901 gttgagcatc tcgatctcga tgatcgattt caccagctgg gcgaactccg ccgcccggcc
      961 caggcgcgag ggaaacggca cctgcttgcc gagcgactcc tgcgcctcct tcggcagggc
     1021 ggcgagcagc ggcgtctcga aaatgcccgg cgcgatcgtg ttgacgcgaa tcccgttgcg
     1081 cgacaggtcg cgcgcgatcg gcagcgtcat gccgacgatg ccgcccttcg aggccgagta
     1141 ggccgcctgg ccgatctggc cgtcgtaggc ggcgaccgag gcggtgttca cgatgacgcc
     1201 gcgctcgccg gaggcgttcg ggccggcctt cgccatcgca tcggcggcca gccggatcat
     1261 gttgaaggtg cctaccacgt tcacggtgag caccttgatg aagcgtccga ggtcgtgcgg
     1321 cgcgtccttg ccgatggtgc gttcggcgag cgcgatgccg gcgcaattga ccagcacgtg
     1381 cagcccgccg aactccttca gcgcagccgc cactgccgcc ttgccgtcgg cttccgacga
     1441 gacatcggtc ttggcgaagc gtccgccgag ctccttcgcc agcttctcgc cggcatcggc
     1501 ctgcaggtcg gcgatgacca ctttgccgcc gccctccgcg gccatgcgcg cggtcgccgc
     1561 gcccagaccg gaagcaccgc cggtgacgag gaatacgctg ttcttgatct gcattaaaac
     1621 tccggaataa ggtaattctt ggagggaggc gcctgatttc gcagataatt ctacccccat
     1681 gcaacttgct ttcggcatcg ctttcgccga cctgtacgac cgcgcagggc tgctgcgcgt
     1741 cgacgccgcc ttcctcgctt tcctgaggga ggccaacgac ggcttgcatg cgcgcctcct
     1801 gtcggcgagg ggcaacttcg cgcagctcaa gccgcgggag gaatccgacc tcctggtcga
     1861 gctggcgccg cacgtcgagg atttcatcgc gcggctcttc ggcatcgagg ccgaggcgcg
     1921 cgcgctcgcc gagcgccaca acgagctcgc gccgctgtat tccgtcaagc ggctcttcgt
     1981 ccagcgccac gccatgcaca aggtgaagcc cgaagacgcg aaaccggacg gctttgcgtt
     2041 cacaaccgag ctcgacttcg cccgccaggt cacggcgtgg cagcaggagg cggcggccaa
     2101 cgccgggaag ctcgaggccg cagcgcgcta cgccgcatgg gcggcgagca cgcccgaagg
     2161 caagcgaaag caccgcggcg gcgtgctgtt caaggcgccg agcaagctcg atttcatgaa
     2221 gctggtgccg gtgcgcaccg aaaccgcacg cggctttgcc gagcacacgc tcgatcacct
     2281 gaggctgcgc gacggcttca agctcaccga cccgggcacc gacctgaccg gggccctcga
     2341 cgaagcgaac tattgcatct ggtgccacga gcagggcaag gactcatgct cgaaggggtt
     2401 gaaggaaaaa gcacccgccg agagcttcaa gaagacggtc ttcggcgtgc cgctcgccgg
     2461 ttgcccgctc gaggagaaga tctccgagtt ccacatggtc aaggtacgcg gccagccgct
     2521 cgccgcgctt ggcatcattg ccgtcgacaa cccaatggtg gcggccaccg gccaccgcat
     2581 ctgcaatgac tgcatgaagt cctgcatcta tcagaagcag gagccggtca atatcccgca
     2641 agccgagacc cgcacgctga aggacatcct ggagctgccg tggggtttcg agatctattc
     2701 gctcttcacg cgctggaatc cattcgatct gcatcgcccc taccccaagc cgcacagcgg
     2761 gcggcgcgtg ctcgtcgtcg gcctgggacc ggccgggttc acgctggccc accacctgat
     2821 gaacgacggt catacggtgg tcggcatcga tggcctgaag atcgagccgc tcgatgcggc
     2881 ggtttcgggc gtggatgcgc tcggccatcg cgtggccttc cgtccgattc gcgacatcgc
     2941 cgagctgcgc gagccgctcg atgggcgtgt gatggcgggg ttcggcggtg tcgccgaata
     3001 cggcatcacg gtgcgctggg acaagaattt tctcaagctg atacgcctgc tgctcgagcg
     3061 ccggg
//
LOCUS       DASYJA010000208         6571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1575372, whole genome shotgun sequence.
ACCESSION   DASYJA010000208 DASYJA010000000
VERSION     DASYJA010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575372"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VGO02_04480"
     CDS             <1..920
                     /locus_tag="VGO02_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEV7476977.1"
                     /translation="APPVTAEPQTTPPWAYTLLRAGMKPPADDGKPQQLPGSKISYSW
                     TEIRNLFSAKDWFPEEHPAMPEVVARGRKPDVYACGMCHYPNGQGKPENASIAGLPAA
                     YIVQQMADYKSGLRKSAEPRMRPPALMLHLAKQTTDAEVRAAADYYAALKYKQWIRLV
                     ETDSVVRTEVIIGSMLAPIEGGGKEPLGQRIIETAEDLERVELRDSRVGFVAYVPTGS
                     IAKGEALAAGGGGRTIACSICHGKDLKGLADVPALAARSPTYMFRQLYDIKSGARAGN
                     GAQLMKAVVEQLTPEDMIALAAYAASRAP"
     gene            complement(927..3380)
                     /gene="pepN"
                     /locus_tag="VGO02_04485"
     CDS             complement(927..3380)
                     /gene="pepN"
                     /locus_tag="VGO02_04485"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEV7476978.1"
                     /translation="MREPQAKAIHLKDYTPPAFSVDTVELDVDIRADHALVRAKLAVR
                     RNAPGPLVLDGDELELVSVLLEGKEPKYTVSAEQLVIADVPERFTLETVTRIVPQKNT
                     KLEGLYATKHGFVTQCEAQGFRRITWFIDRPDVMARYTTTVRADPKKYPVLLSNGNLV
                     ASGDGWAKFEDPFPKPCYLFALVAANLEVLREGNLYIYVEPGKLDQAGWAMDCLKRAI
                     AWDEKRFGLKLDLKEYKIVAVSDFNSGAMENKGLNIFNTKYVLARADTATDFDYLAID
                     RVVAHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEYGADIYSRAVTRIQEVRGLRS
                     SQFAEDAGPMAHPVRPQSYVEIRNFYTMTVYEKGAEVVRMQHTLLGEEKFQAGMRLYF
                     QRHDGHAVTCDDFVQAMQDASGVDLTQFKRWYDVAGTPVLDVRGSFSGSTYTLTVKQS
                     MNPPFHIPFAVKVGPHEKLLSLKRPEEQYVFEGLKERPVPSLLRGFSAPVILKYPYTE
                     AELLHLMANDDDAFNRWEAGQRLATEAILEKAGKPSPAFLEAARRLLAHPDPAFAAEA
                     LALPAESFLAEQMEVVDPDRLHESRNALRREMARALKGDFLQTYEKLKSTGAYSPDPA
                     SAGRRALRNLCLNYLGELGMSALAYEQFKRADNMTDSMGALSVLANLDCAERQPALDA
                     FYARWHDEPLVVDKWLAVQASSRLPGTLFNVIKLLKHPAFDIKVPNKVYALIRAFGSN
                     HVRFHAADGGGYAFLAEQTIALDALNPQVAARMARAFDRWKKFDEGRKAHARRQLERV
                     RDTQGLSKDVAEIVSKALT"
     gene            3532..3948
                     /locus_tag="VGO02_04490"
     CDS             3532..3948
                     /locus_tag="VGO02_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin family protein"
                     /protein_id="HEV7476979.1"
                     /translation="MTPQQIALIQESWRKVLPISDTAAELFYSRLFTLDPALKPLFKG
                     DIKLQGRKLMSMLGMVVSGLSSFDRLAPAVKELGRRHDAYGVRPLHYRTVAEALLWTL
                     KQGLGDAFTEETELAWVAAYGNIAALMQSKQKAVAP"
     gene            3948..4523
                     /locus_tag="VGO02_04495"
     CDS             3948..4523
                     /locus_tag="VGO02_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEV7476980.1"
                     /translation="MRVLVVDESPERAELLREALVHAGHEVTATLSSPLALLKTIDEL
                     KPDVIVIDMDSPSRDVLEHLVFVSQHTPRPVVMFAQDDAPETIRAATRAGVSAYVVDG
                     LDRNRIKAIVEAAVARFEDYQALRTQLAEANLKLSERKAVEKAKGLLMKSRGLDEDAA
                     YAALRKMAMDRKLKLVEVAQKVIEAADLLGA"
     gene            4709..5938
                     /locus_tag="VGO02_04500"
     CDS             4709..5938
                     /locus_tag="VGO02_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV7476981.1"
                     /translation="MKKRDFLKAAGAGGLMSMVSEGVRVGAWAQGSDAPEKKEVKIGF
                     IPLTDCSSVVMASVMEFDKKYGIKIIPSKEASWAAVRDKLVNGELDAAHVLYGLIYGV
                     QMGLGGPKKDMNVLMNLNHNGQAFTLSRKLYDKGVKDGTSLNTLIRKEKAEYTFAQTF
                     PTGTHAMWIYYWLAAHGIHPFQDVKTIVVPPPQMVANMRVGAMDGFCVGEPWNYRAIM
                     DNIGFTANTTQDLWQDHPEKTLGCTADFTAKYPNTARAMTAAVIDAGRWIDSSLSNRQ
                     KTAEVVSDKSYVNCEKDVIVARMLGRYDNGIGKVWDDKNYMKFYNEGHVTFPYLSDGM
                     WFMTQHRRWGLLKEDPDYLAVAQKVNRIDIYKQAAAMTKTPLPKEPMRTARLMDGVVW
                     DARNAKTYAGAFKLKVA"
     gene            6071..>6571
                     /locus_tag="VGO02_04505"
     CDS             6071..>6571
                     /locus_tag="VGO02_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV7476982.1"
                     /translation="MSVIACAAGLAAFVAAWAAIAKFGGRIPDPLSVAAAAAKIFADP
                     FYSKGPNDQGIGWNVLSSLRRVGIGFGLAALVGIPLGFIVGRFRFASGMAAPIIAMLK
                     PVSPLAWLPIGLLVFKAANPAAIYVIFVCSLWPMIVNTAVGVRQIPQDYLNVARVLNL
                     SEWKVFT"
BASE COUNT         1204 a         2064 c         2212 g         1091 t
ORIGIN      
        1 gagcaccgcc ggtcaccgcg gagccgcaga cgacgccacc gtgggcttac acgctgctac
       61 gtgccggaat gaagccgccg gcggacgacg gcaagccgca gcagctgccg ggaagcaaga
      121 tcagctactc gtggaccgag atccgcaacc tgttcagcgc aaaggactgg tttccggaag
      181 agcatccggc gatgcccgaa gtggtagcgc gcgggaggaa gccagatgtc tacgcgtgcg
      241 ggatgtgcca ttacccgaac ggccagggca agcccgaaaa cgcaagcatc gccggcctgc
      301 ctgcggcgta catcgtccag cagatggcgg actacaagag cgggctgcgc aaaagcgccg
      361 aaccgaggat gcggccgccc gcgctgatgc tgcatctggc gaagcagacg accgatgccg
      421 aggtgagggc tgccgcggac tactacgccg ctcttaagta caagcagtgg atccgcttgg
      481 tcgagaccga ctccgtggtg cgcaccgagg tgatcatcgg ctcgatgctc gcgccgatcg
      541 aaggcggcgg caaggagccg ctcggccagc gcatcataga gaccgccgag gatctcgagc
      601 gggtggagct gcgcgactcg cgtgtcggct tcgtcgccta cgtacccacc ggcagcatcg
      661 ccaaaggcga ggcgctcgcg gctggcggcg gaggcagaac catcgcgtgc tccatctgcc
      721 acggtaagga ccttaaaggc ctcgccgacg tgccggcgct cgcggcgcgc tccccgacct
      781 acatgttccg ccagctctac gacatcaaga gcggcgcccg tgccggaaac ggggcgcagc
      841 tcatgaaagc ggtagtcgag cagctcacgc ccgaggacat gatcgcgctt gcggcctacg
      901 cagcgtcgcg cgcgccctga aaatgcctac gtcagcgcct tgctgacgat ctcggcgacg
      961 tccttcgaca ggccctgcgt gtcgcgcacg cgctcgagct ggcggcgcgc gtgggccttc
     1021 ctgccctcgt cgaacttctt ccagcggtcg aaggcgcgcg ccatgcgggc cgccacctgc
     1081 gggttgagcg cgtcgagcgc gatcgtctgc tcggcgagga acgcgtagcc gccgccgtcg
     1141 gcggcgtgga agcgcacgtg gttggagccg aaggcgcgga tcagggcgta gaccttgttc
     1201 ggcaccttga tgtcgaacgc gggatgtttc agaagtttaa tgacattgaa aagagttcct
     1261 ggcagccgtg acgacgcctg caccgcgagc cacttgtcga ccaccagcgg ctcgtcgtgc
     1321 cacctcgcgt agaaagcgtc gagcgcgggc tggcgctccg cgcagtccag attggcgagc
     1381 acgctgagcg cgcccatcga gtcggtcatg ttgtcggcgc gcttgaactg ctcgtacgcc
     1441 agggccgaca tgccgagctc gccgaggtag ttcaggcaca gattgcgcag cgcgcgcctg
     1501 ccggccgagg ccgggtcggg ggaatacgcg cccgtgctct tcagcttctc gtaggtctgc
     1561 aggaaatcgc ctttcaatgc gcgcgccatt tcccggcgca gcgcgttcct cgattcgtgc
     1621 agccggtcgg ggtcgaccac ctccatctgc tcggcgagga agctctcggc gggcagcgcg
     1681 agcgcctcgg ccgcgaaggc cgggtcggga tgcgcgagga gcctgcgcgc cgcttcgagg
     1741 aaggcgggcg agggcttgcc ggccttctcc aggatcgcct cggtggcgag ccgctggccg
     1801 gcttcccagc ggttgaacgc gtcgtcgtcg ttcgccatca ggtgcagcag ctccgcctcg
     1861 gtgtacggat atttcaggat caccggcgcc gagaagccgc gcagcaggga cggcaccggc
     1921 ctctctttca aaccctcaaa tacgtattgc tcctccggac gcttgagcga cagcagtttt
     1981 tcgtgaggac cgaccttcac tgcgaaaggt atgtggaagg gagggttcat cgactgcttc
     2041 accgtcaagg tgtaggtgct tcccgaaaaa gaaccccgga catcgagaac cggcgtgccg
     2101 gcgacgtcat accagcgctt gaactgggtg aggtcgacgc cggaagcgtc ctgcatagcc
     2161 tgcacgaagt cgtcgcaggt caccgcgtgg ccgtcgtggc gctggaagta cagccgcatt
     2221 ccggcctgga acttctcctc gccgagcagc gtgtgctgca tgcggacgac ctcggcgcct
     2281 ttctcgtaca ccgtcatggt gtagaagttg cggatctcga cataggactg cgggcgcacc
     2341 gggtgcgcca tcgggccggc gtcctcggcg aactgcgacg agcgcaggcc gcgcacctcc
     2401 tggatgcgcg tgacggcgcg cgagtagatg tcggcgccgt actcctggtc gcggaacacg
     2461 gtcaggcctt ccttgagcga aagctggaac cagtcgcggc aggtgacccg gttgccggtc
     2521 cagttgtgga agtactcgtg cgcgaccacg cggtcgatgg ccaggtagtc gaagtcggtg
     2581 gcggtgtcgg cgcgcgccag gacgtacttg gtgttgaaga tgttgaggcc cttgttctcc
     2641 atcgcgccgg agttgaagtc gctgaccgcg acgatcttgt actccttcag gtcgagcttc
     2701 aggccgaagc gcttctcgtc ccaggcaatg gcgcgcttca ggcagtccat cgcccagccc
     2761 gcctgatcga gcttgcccgg ctcgacgtag atataaaggt tgccctctcg aagcacctcg
     2821 agattcgccg cgaccagcgc gaacaggtag cagggcttcg ggaacggatc ctcgaatttc
     2881 gcccagccgt ctcctgaagc gacgagattg ccgttggaaa gcagcaccgg atacttcttc
     2941 gggtcggcgc gcacggtggt ggtgtagcgc gccatgacgt cgggccggtc gatgaaccag
     3001 gtgatgcggc ggaagccctg cgcctcgcac tgcgtgacga agccatgctt ggtcgcgtac
     3061 aggccttcga gcttggtgtt cttctgcggc acgatgcgcg tcacggtctc gagcgtgaag
     3121 cgctccggca catcggcgat gacaagctgc tccgcgctca ccgtgtactt cggttctttg
     3181 ccctccagaa ggactgaaac cagctcgagc tcgtcgccgt cgagcaccag cgggccgggc
     3241 gcattgcggc gcacggcgag cttcgcgcgc accagggcgt ggtctgcgcg gatatcgaca
     3301 tccaactcga cggtgtcgac gctgaacgcg ggcggggtgt agtccttgag gtggatggct
     3361 ttcgcctgcg gttctctcat cccggtattt tagtggtgca ctgccgcggc cgtcgccccg
     3421 gcttagtgcg cgccagccca gggcggaccc tgattttcaa gggaaaactg ttggcatgca
     3481 ccttgcgaaa cagagaagcg aggagaggcc aacacgggag gtgacggaaa catgactccc
     3541 cagcagatcg ctctcatcca ggagagctgg cgcaaggtcc tccccatctc cgacaccgcg
     3601 gcagagctct tctacagccg cctcttcacg ctcgaccccg cgctcaagcc gctgttcaag
     3661 ggggacataa agctgcaggg gcgcaagctg atgtcgatgc taggcatggt ggtgtccggg
     3721 ctaagcagct tcgaccggct cgcgccggcg gtgaaggagc tgggccgcag gcacgatgcg
     3781 tacggcgtgc ggccactgca ctaccgcact gtggctgagg cgctgctgtg gacgctgaag
     3841 caggggctgg gcgacgcgtt caccgaggaa accgagctcg cctgggtcgc cgcctacggc
     3901 aacatcgccg cgctcatgca gagcaagcag aaggccgtcg cgccctgatg cgcgtcctgg
     3961 tggtcgacga gtcgcccgag cgcgccgagc tgctgcgcga ggcgctggtg cacgccggcc
     4021 acgaggtgac ggcgaccctc tcctcgccgc tcgcgttgct gaagaccatc gacgagctca
     4081 agcccgacgt cattgtcata gacatggact cgcccagccg cgatgtgctc gagcacctgg
     4141 tcttcgtcag ccagcacacg cctcgcccgg tggtcatgtt cgcgcaggac gacgcgccgg
     4201 aaacgatccg cgccgcgacg cgcgccggcg tctcggccta cgtggtggat ggcctcgacc
     4261 gcaaccggat caaggcgatc gtggaggcgg cggtggcgcg cttcgaggac taccaggcgt
     4321 tgcgcacgca gcttgccgag gcaaacctca agctctccga gcgcaaggcg gtggaaaagg
     4381 cgaagggctt gctgatgaag agccgcggcc tcgacgagga cgccgcctat gcggcgctcc
     4441 ggaaaatggc catggaccgc aagctgaagc tcgtcgaggt ggcgcagaaa gtgatcgagg
     4501 ccgcggatct tttgggtgca tgacgcacgc tgttagtgca aggctctttc gaaaagatca
     4561 atcaaatcaa taaaagcgcc ttggcatagc atttgcgtag agaagcctga gcgggcgcaa
     4621 cggcgcgccc tgcgtgaacg aacgaaggcg ttcggaattt cgcgaaagcg gaagcccgaa
     4681 cgcctttttg tttttggaaa gggatgcgat gaaaaaacgg gacttcctga aagcagccgg
     4741 agccggaggg ctcatgagca tggtttctga aggcgtgcgt gtcggcgcgt gggcgcaggg
     4801 ctcggacgcg ccggagaaga aggaagtgaa gatcggcttc atcccgctta ccgactgctc
     4861 gtcggtggtg atggcctcgg tgatggagtt cgacaagaag tacggcatca agatcatccc
     4921 ctcgaaggaa gcttcctggg ccgcggtgcg cgacaagctg gtgaacggcg aactcgacgc
     4981 ggcgcacgtc ctttacggcc tgatctacgg cgtgcagatg ggcctgggcg ggccgaagaa
     5041 ggacatgaac gtcctcatga acctgaacca caacggccag gcgttcacgc tgtcgcgcaa
     5101 gctctacgac aaaggcgtca aggacggcac ttcactgaat accctgatca ggaaggaaaa
     5161 ggccgaatac acattcgccc agaccttccc gaccggcacg catgccatgt ggatctacta
     5221 ctggctcgcc gcgcacggca tccacccttt ccaggacgtg aagacgatcg tcgtgccgcc
     5281 gccgcagatg gtggccaaca tgcgcgtcgg cgcgatggac gggttctgcg tcggcgagcc
     5341 gtggaactac cgagcgatca tggacaacat agggttcacc gcgaatacca cgcaggacct
     5401 gtggcaggac cacccggaga agacgctggg gtgcaccgcc gacttcacgg cgaagtatcc
     5461 caacaccgcg cgggcgatga ccgcggccgt gatcgacgcc ggccgctgga ttgacagctc
     5521 gctctccaat cgccagaaga cggccgaggt ggtctccgac aagtcctacg tgaactgcga
     5581 gaaggacgtc atcgtcgcgc gaatgctcgg ccgctacgac aacggcatcg gcaaggtgtg
     5641 ggacgacaag aactacatga agttctacaa cgagggccac gtcaccttcc cgtacctgtc
     5701 cgacggcatg tggttcatga cgcagcaccg gcgctggggg ctgctgaagg aggacccgga
     5761 ctacctcgcg gtggcgcaga aggtgaaccg catcgatatc tacaaacagg cggcagcgat
     5821 gacgaagacg ccgctgccga aagaacccat gcgcaccgcc aggctgatgg atggcgtggt
     5881 gtgggacgcg aggaacgcca agacctacgc cggcgcgttc aagctcaagg tggcgtgaat
     5941 ggcggcggtc gcgctgagaa aagaactggt tgccatcaag accagcccgc ctcatccggc
     6001 cgcggttgcc gccatcccag catccaggac cgagacgggc tatcggcgcg tgaaggcgcc
     6061 ggaatggctg atgagcgtca tcgcctgcgc cgccggcctc gcggcgttcg tcgccgcgtg
     6121 ggcggcgatc gccaagttcg gcgggcgcat tccggacccg ctcagcgtcg cggcggcggc
     6181 ggcgaagatc ttcgccgacc ccttctacag caaggggccg aacgaccagg gcatcggctg
     6241 gaacgtgctt tcgtcgctca ggcgcgtcgg catcggcttc ggcctcgccg cgctggtcgg
     6301 aatcccgctg ggcttcatcg tcgggcggtt ccgcttcgcc tccgggatgg cggcgccgat
     6361 catcgccatg ctgaagccgg tgtcgccgct cgcctggctg ccgatcgggc tgctggtgtt
     6421 caaggcggcg aatccggcgg cgatctacgt gatcttcgtc tgcagcctgt ggccgatgat
     6481 cgtgaacacc gcggtcggcg tgcgccagat cccgcaggat tacctgaacg tggcgcgcgt
     6541 gctgaacctc tccgagtgga aggtgttcac c
//
LOCUS       DASYJA010000209         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1756282, whole genome shotgun sequence.
ACCESSION   DASYJA010000209 DASYJA010000000
VERSION     DASYJA010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756282"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="VGO02_04510"
     CDS             <1..938
                     /locus_tag="VGO02_04510"
                     /inference="COORDINATES: protein motif:HMM:NF021633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HEV7476983.1"
                     /translation="ARILKRAWQVYVAHVFIFVIFMAEIAYLTRSIDNPLYNEEMGAL
                     DFMKEPGDTLIQALLLKFKPVFMDVLPLYIVLLLAFPPVLWLLLRVPTLALAGSVAVY
                     AASWEFAWNLPSYPTGVWYFNPFAWQLIFVFGAWCGVGGTTWMRPFLRSRWVLGLAAA
                     YLGIAFLIVMTWYFPRYAVYIPKAVQDWMYPLDKTNMDVLRFIHFLALAVLTVYFVPA
                     DARWLKSPLVRPMILCGQHSLEIFCLGIFLSFLGHFITSEYSRSIVVQILISTLGILT
                     MVAVAALISWYKDVEGRGAGPRSKPPDADLAGGAA"
     gene            935..>1501
                     /locus_tag="VGO02_04515"
     CDS             935..>1501
                     /locus_tag="VGO02_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476984.1"
                     /translation="MKALAGALVGVIAYATAASGAGDACNVASHLVQVDAPLPRVAEA
                     IDKTKSLTIVVAGTTSSSLPGAEGAKLAWPARLEGALAKKFPGVAVKVVPYVAPRQTA
                     EVMAQTFPKMLKADKPQLVVWQTGTYDAMQGIGTEPFQTVLEQAVDVLHEGGADVIFM
                     NMQFSPRTEAVLASQAYAEAIRWVALEHA"
BASE COUNT          227 a          491 c          483 g          300 t
ORIGIN      
        1 gcgcgcgcat cctcaagcgc gcctggcagg tctacgtcgc gcatgtcttc attttcgtga
       61 tcttcatggc ggagatcgca tatctcacgc gcagcatcga caacccgctc tataacgaag
      121 aaatgggcgc gctcgacttc atgaaggagc cgggcgacac gctgatccag gcgctgctct
      181 tgaaattcaa gcccgtcttc atggacgtgc tgcctcttta tatcgtgctg ctgctcgcgt
      241 tcccgccggt gctgtggctg ctcctgcgcg tgccgacgct ggcgctcgcg ggctcggtcg
      301 ccgtctatgc ggcgagttgg gagttcgcct ggaacctgcc gtcctatccg accggcgtct
      361 ggtacttcaa tccgttcgcc tggcagctca tcttcgtatt cggcgcctgg tgcggcgtcg
      421 gcggcaccac gtggatgcgg ccgttcctgc gctcgcgctg ggtgctcggc cttgccgccg
      481 cttatctggg gatcgcgttc ctcatcgtga tgacgtggta cttcccgcgc tacgcggtct
      541 acatcccgaa ggcggtgcag gactggatgt acccgctcga caagaccaac atggacgtgc
      601 tgcgcttcat ccacttcctc gcgctcgccg tgctgacggt ttacttcgtg cccgccgacg
      661 cgcgctggct caagtcgccg ctggtgcggc cgatgatcct gtgcgggcag cactcgctcg
      721 agatcttctg cctcggcatc tttctctcgt tcctcgggca cttcatcacg tcggaatact
      781 cgcgctccat cgtggtgcag atactcatca gcacgttagg gatattgacg atggtcgccg
      841 ttgctgcgtt gatctcgtgg tacaaggacg ttgaagggcg tggcgccggg ccacgctcca
      901 agccgccgga cgcggacctc gcgggaggag cggcatgaag gccctagccg gggcgctggt
      961 gggcgtcatc gcctatgcga ccgccgcgtc gggggcgggc gacgcctgca acgttgcctc
     1021 gcatctcgtc caggtcgacg ccccgctgcc gcgtgtcgca gaggcgatcg acaagaccaa
     1081 gagcctcacc atcgtggtcg ccgggacgac gtcctcgtcg ctgcccggcg cggagggggc
     1141 caagctcgcc tggccggcgc ggcttgaagg ggcgttggcc aagaagtttc ccggcgtggc
     1201 ggtgaaggtc gtgccctacg tcgcgccgcg ccagaccgcc gaagtaatgg cgcagacctt
     1261 tcccaagatg cttaaggccg acaagccgca gttggtcgtc tggcagacgg gaacctacga
     1321 cgcgatgcaa ggcatcggca cggaaccgtt ccagactgtg ctcgaacagg ccgtcgacgt
     1381 gctccatgaa ggcggcgcgg acgtcatttt catgaacatg cagttcagcc cgcgcaccga
     1441 agcggtgctc gcctcacagg cctatgcgga ggcgatccgc tgggtcgcgc tcgagcatgc
     1501 c
//
LOCUS       DASYJA010000210         2825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1302664, whole genome shotgun sequence.
ACCESSION   DASYJA010000210 DASYJA010000000
VERSION     DASYJA010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302664"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VGO02_04520"
     CDS             complement(<1..562)
                     /locus_tag="VGO02_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEV7476985.1"
                     /translation="MRPVDTEVLVVGAGPVGLTLAIALGQAGVRCTLVERHRSPQFLP
                     KMERCNARTMEIFRRMGIAAKVRAAGLRADVPMDVWLILAMNRAPLLRHAYPSVDEAR
                     GQIDACTDGSLPLEPYQLISQYTLEPLLKSIAETLPSVTLRYGDEFLSFEQDDQGVAS
                     RVKNGTIHSKYLVGCDGGSSIVRQQLG"
     gene            586..1536
                     /locus_tag="VGO02_04525"
     CDS             586..1536
                     /locus_tag="VGO02_04525"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7476986.1"
                     /translation="MRRILAALALICTAASAQDYPSRPVHLVVPYTPGTGADLLARIL
                     SVKLGERWKAPVVVDNRPGATGNIGTDLVAKSAADGYTLLLAATSFSTNPSLTAPLPY
                     DPVKSFAPVVLVATSTLAVVMNPEMPAQTFAEFLDLVKRQPGKLYYGSPGNGGIQHLA
                     MELLKLETGMNLVHVPYKGLGGAMSDVIAGHIPVMISATQSAAPHVRSGKLRMLVLMS
                     AERQAAFPGVPTLHELGYRDLDVDTWYALFAPAGAPPGVVARVNADVNLFLKDAETRE
                     LLAKQGLNPGGGTPAQLGAMVERELARWSRVVSAAGIKAD"
     gene            1537..2238
                     /locus_tag="VGO02_04530"
     CDS             1537..2238
                     /locus_tag="VGO02_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEV7476987.1"
                     /translation="MSELEDLVAAYRILAAHGVIDAYGHVSLRSPRNPQRYYVARSLA
                     PELVTEADLIEYDLDSRPLEERGRESVRERFIHGEIYKTRPEVMAVVHNHSPSVVPFS
                     VTGVPMRAIFHMAAFVGDGLPNFEIRDVKPGSDLLVKDAFLGKALANTLGAKPAALMR
                     GHGAVVVGENLPRAVGRSVYLELSARLQMQAMALAGPGGKITYLDADEVKASDPVQNY
                     ARAWPMWRAKALGKL"
     gene            2235..>2825
                     /locus_tag="VGO02_04535"
     CDS             2235..>2825
                     /locus_tag="VGO02_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEV7476988.1"
                     /translation="MSELCSLTAVQLLEGYRTGAFSPVEVAASVMRRIEERNPQLNAF
                     NLVSNHVNEEAKASESRWLRGVPKGLLDGVPVSIKDIILTRGWPTLRGSKTVDPRGPW
                     TEDAPATARLREHGAVLLGKTTTPEFGWKGVTDSPLTGITRNPWNPAKTPGGSSGGAA
                     AAVAAGMGPLAVGTDGGGSIRIPCSFTGLFGIKPSFG"
BASE COUNT          457 a          988 c          948 g          432 t
ORIGIN      
        1 tgccgagctg ctgccggacg atgctggaac ctccgtcgca gcccaccaaa tactttgagt
       61 ggatggttcc gtttttcacg cgggaagcaa cgccctgatc gtcctgttcg aaagacagga
      121 attcatcgcc gtagcgaagc gtgacgcttg gcagcgtttc ggcaatcgac ttgagcagcg
      181 gctccagggt gtactgcgag atgagctggt agggctcgag cggcagcgag ccgtcggtac
      241 acgcgtcgat ctgcccgcgc gcctcgtcca ccgaaggata ggcgtggcgc agcagcggcg
      301 cccggttcat cgccaggatc agccagacgt ccatcggcac gtcggcgcgc aggccggcgg
      361 cgcgcacctt ggccgcgatg cccatgcggc ggaagatctc catcgtgcgc gcgttgcagc
      421 gctccatctt cggcaggaat tgcggcgacc ggtggcgctc caccaacgtg cacctgacgc
      481 ccgcctgacc gagcgcgatg gcgagcgtga ggccgaccgg tcccgcgccg acgacgagga
      541 cctcggtatc gacggggcgc atggggcggt gattataatc agcgcatgcg gaggatcctc
      601 gcggcgctcg ccctgatctg cactgcggcg agcgcgcagg actatccgtc gcgcccggtc
      661 cacctcgtcg ttccctacac gcccggcacc ggcgccgacc tcctcgcgcg catcttgagc
      721 gtcaagctcg gcgagcgctg gaaagctccg gtggtagtgg acaaccggcc cggcgccacc
      781 ggcaacatcg gcaccgacct cgtggcgaag agcgcagccg acggttacac gctgctgctc
      841 gccgcgacgt cgttctccac caacccttcg ctcaccgcgc ccctgcccta cgacccggtg
      901 aagagcttcg cccccgtcgt cctggtggcg accagcacgc tcgccgtggt gatgaatccc
      961 gaaatgccgg cgcagacctt tgccgagttc ctcgacctgg tgaagcgcca gcccggcaag
     1021 ctctactacg gctcgccggg caacggcggc atccagcacc tcgcgatgga gctgctcaag
     1081 ctggaaaccg gcatgaacct ggtgcacgtg ccctacaagg gcctcggcgg cgcgatgtcg
     1141 gacgtgatcg ccggccacat cccggtgatg atctcggcga cgcagtcggc cgcgccccac
     1201 gtgcgctccg ggaagctgcg catgctggtg ctgatgagcg ccgagcgcca ggccgccttt
     1261 ccgggcgtgc cgacgctgca cgagctgggc taccgcgatc tcgacgtgga cacctggtac
     1321 gcgctgttcg cgccggcggg cgctccgccc ggcgtggtgg cgcgcgtcaa cgccgacgtc
     1381 aatcttttcc tcaaggacgc cgagacccgc gaactgctgg cgaagcaggg tctcaacccg
     1441 ggcggcggca ctcccgccca gctcggcgcg atggtcgagc gcgagctcgc ccgctggtcg
     1501 cgcgtcgtct ccgccgccgg catcaaggcc gactaaatga gtgagctcga agacctggtg
     1561 gcggcgtacc gcatcctcgc ggcgcatggc gtgatcgacg cctatgggca cgtcagcctg
     1621 cgctcgccgc gcaatccgca gcgctactac gtcgcacgct cgctcgcccc cgagctggtg
     1681 acggaggcgg acctgatcga gtacgacctc gacagccggc cgctcgagga gcgcggacgc
     1741 gagtcggtgc gcgagcgctt catccatggc gagatctaca agacgcgccc cgaggtgatg
     1801 gcggtggtgc acaaccattc gccctcggtg gtgcccttca gcgtcaccgg cgtgccgatg
     1861 cgcgcgatct tccacatggc ggccttcgtc ggcgacggcc tgccgaactt cgagatccgc
     1921 gacgtgaaac caggctcgga tcttctcgta aaggatgctt ttttagggaa agcattagcc
     1981 aacaccctgg gcgcgaaacc ggctgccctg atgcgcggcc acggcgccgt ggtcgtaggc
     2041 gagaacctgc cgcgcgcggt cgggcgcagc gtctacctcg agctgagcgc gcgcctgcag
     2101 atgcaggcaa tggcgctggc cggtcccggc ggcaagatca cctacctgga cgcggacgag
     2161 gtgaaggcgt ccgacccggt gcagaactac gcgcgcgcct ggccgatgtg gcgcgccaag
     2221 gcgctcggca agctatgagc gagctgtgct cgctcaccgc ggtccagctc ctcgaaggct
     2281 atcgcaccgg ggcgttctcg ccggtcgagg tcgccgcctc ggtcatgcgg cgcatcgagg
     2341 agcgaaatcc gcagctcaac gcattcaacc tcgtttcaaa tcacgtaaac gaagaggcaa
     2401 aggcgtccga atcgcgctgg ctgcgcggcg tgccgaaagg gctgctcgac ggcgtgccgg
     2461 tatcgatcaa ggacatcatc ctcacccgcg gctggccgac gctgcgcggc tcgaagaccg
     2521 tcgacccgag agggccctgg actgaagatg cgcccgcgac ggccaggctg cgcgagcacg
     2581 gcgcggtgct gctcggcaag accaccaccc ccgagttcgg ctggaagggc gtgaccgaca
     2641 gcccgctcac cgggatcacg cgcaacccgt ggaacccggc gaagacgcct ggcgggtctt
     2701 caggcggcgc cgcggccgcc gtcgccgcgg gcatggggcc gcttgccgtc ggcaccgacg
     2761 gcggcggctc gatccggatt ccctgcagct ttaccggcct cttcggcatc aagccgtcgt
     2821 tcggc
//
LOCUS       DASYJA010000211         2063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_122452, whole genome shotgun sequence.
ACCESSION   DASYJA010000211 DASYJA010000000
VERSION     DASYJA010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122452"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VGO02_04540"
     CDS             complement(<1..923)
                     /locus_tag="VGO02_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEV7476989.1"
                     /translation="MRKGFGLFAGALAGLVLANGAAAAWEPTKPVEIVVAAGAGGASD
                     QMARMIQAAIQKNNLMKQPMVVSLKGGASGAEALMYMKNSGGDANKVLIAYSLIYMLP
                     LSAKIPFNWRELTPVAVVALDQFVLWTNAQLPYKTVKEFADAAKGANPTFKMGGTGSK
                     REDHVLTVFLEKKTGAKFAYLPYKSGGEAATQLVGNHTQSNVNNPSENLEVWRAGQVR
                     PLCVFDKERIQYKNKVAGEMSWGDIPTCREQGVDVQYLMLRAMFLPGKVTPDQTTFYV
                     DLFKKVSETAEYKDYMEKQALKPIFLTGKDML"
     gene            complement(955..>2063)
                     /locus_tag="VGO02_04545"
     CDS             complement(955..>2063)
                     /locus_tag="VGO02_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEV7476990.1"
                     /translation="DHRGPEFGRLGLELLDGLKAVFKTRQPVVIYPASGTGAWEAALV
                     NSLSAGDRVLMAETGHFAALWQKMAAKLGIEVEFLPGDWRHGADPEAIEKRLSSDRDQ
                     QLKAVCVVHNETSTGVASRIPELRKAIDAARHPAIFMVDTISSLASIDFRHDEWGVDV
                     TVAGSQKGLMLPPGLSFNCISKKALAASKSARLPRSYWAWDEMIANGKGGYFPYTPAT
                     NLLYGLREALKMLLDEEGLDAVFARHQRHAEATRRAVRAWGLEVFALDPREYSASLTG
                     VLMPAGHDSDRVRKVILEAFDLSLGTGLTKLGGKMFRIGHLGDFNDLMLMGTLAGVEM
                     GLALAGVPAKKGGVEAAMDYLAASAKAAPARQAA"
BASE COUNT          347 a          678 c          648 g          390 t
ORIGIN      
        1 agcatgtcct tgccggtgag gaagatcggc ttgagcgcct gcttctccat gtagtccttg
       61 tattcggccg tctcgctgac ctttttgaag agatcgacat agaacgtcgt ctgatcgggc
      121 gtcacctttc cgggcaggaa catcgcgcgc agcatgaggt actgcacgtc gacgccctgc
      181 tcgcggcagg tggggatgtc gccccaggac atctccccgg ccaccttgtt cttgtactgg
      241 atgcgttcct tgtcgaacac gcagagcgga cgcacctgtc ccgcgcgcca gacttccagg
      301 ttctccgagg gattgttgac gttcgactgg gtatggttgc cgacgagctg cgtcgcggcc
      361 tcgccgccgg acttgtaggg cagataggcg aacttcgcgc cggtcttctt ttccaggaac
      421 accgtgagga cgtggtcttc gcgcttggac cccgtgccgc ccatcttgaa cgtcgggttc
      481 gcgcccttcg ccgcgtcggc gaactccttg accgtcttgt agggaagctg cgcattcgtc
      541 cacagcacga actggtcgag cgccaccacc gccaccggcg tgagctcgcg ccagttgaac
      601 ggaatcttcg ccgaaagcgg aagcatgtag atgagcgaat aggcgatcag caccttgttg
      661 gcgtcgccgc cgctgttttt catgtacatg agggcttccg cgcccgaggc gccgcccttc
      721 aacgaaacca ccatcggctg cttcatcagg ttgttcttct ggatggcggc ctggatcatc
      781 ctcgccatct ggtcggaagc cccgccggcg cccgctgcta cgacgatctc gaccggcttg
      841 gtcggctccc aagctgcagc ggcgccgttg gcgagtacca ggccggcaag cgcgccggcg
      901 aatagaccga aacctttcct catgtcgctc ctcctcctgg atttgtagcg aaactcaagc
      961 ggcttggcgc gcgggggccg ccttcgcgct tgccgcaaga tagtccatcg ccgcctcgac
     1021 accgcccttc ttcgccggca cgccggcgag cgcgaggccc atctcgactc cggcgagcgt
     1081 gcccatcagc atgaggtcgt tgaagtcccc caagtggccg atgcggaaca tcttcccgcc
     1141 gagcttggtg agaccggtgc cgagtgacag gtcgaacgcc tcgaggatca ctttgcgtac
     1201 ccgatcggag tcgtgtcccg cgggcatcaa cacgccggtg agcgaggcgg aatactcgcg
     1261 cgggtcgagc gcgaagacct cgaggcccca ggcgcgcacc gcgcgccggg tcgcctcggc
     1321 atggcgctga tgccgcgcga ataccgcgtc gagcccttcc tcgtcgagca gcatcttgag
     1381 cgcttcgcgc aggccgtaca gcagattggt agcgggcgta taggggaaat agccgccctt
     1441 gccattggca atcatctcgt cccacgccca gtagctgcgc ggcaggcgcg cggacttgga
     1501 agctgctagc gccttcttgg agatgcagtt gaacgaaagg ccggggggaa gcatgaggcc
     1561 cttctgcgaa ccggccaccg tcacgtccac cccccattcg tcgtggcgga agtcgatcga
     1621 ggcgagcgag gagatcgtgt cgaccatgaa aatcgcggga tgcctggccg catcgatggc
     1681 ttttcgcagc tctggaatgc gcgaagcgac gccggtcgag gtctcgttgt gcaccacgca
     1741 aacggctttg agctgctggt cgcggtcgga gctcaagcgc ttctcgatcg cttccggatc
     1801 ggcgccatgg cgccagtcgc ccggcaggaa ttcgacttcg ataccgagct ttgccgccat
     1861 cttctgccaa agcgccgcga aatgaccggt ctcggccatc agcacgcgat cgccggccga
     1921 gagcgagttc acgagtgccg cttcccaggc cccggtgccc gaagccgggt agatcaccac
     1981 aggctgcctc gtcttgaaca cggccttgag gccatccagg agctcgagtc cgagccggcc
     2041 gaactcgggg ccgcgatggt cca
//
LOCUS       DASYJA010000212         2789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3119639, whole genome shotgun sequence.
ACCESSION   DASYJA010000212 DASYJA010000000
VERSION     DASYJA010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3119639"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..404)
                     /locus_tag="VGO02_04550"
     CDS             complement(183..404)
                     /locus_tag="VGO02_04550"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEV7476991.1"
                     /translation="MKPFNFMAGIKGKSFSVAELEAVGVRRISVATSLYRAAMSGLVE
                     AAREVKDKGSFGYLERTISTPDLNGFMRG"
     gene            526..924
                     /locus_tag="VGO02_04555"
     CDS             526..924
                     /locus_tag="VGO02_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7476992.1"
                     /translation="MASKQLAVLATLLGIATPGHAFDTRNEAQTVMFYYAIPLDAHSK
                     KDRAPWLGMQINGKRDYQSYSMDSKLLTFNFEESGSAAASALIIGAVAVGAVAVVGSR
                     GKSSQEQVQQKQASTPQPPAPVPCPTDCGK"
     gene            956..1396
                     /locus_tag="VGO02_04560"
     CDS             956..1396
                     /locus_tag="VGO02_04560"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HEV7476993.1"
                     /translation="MAYEPHIPEIAAVIQLTVAPVFMLTAVGTIIGALNIRLGRAVDR
                     RHELEEELTRMPADEKPSAHQELGFIARRIRFVYYSILAAVVSGLFTCLLIAGAFLAT
                     FLRSDISHTIGTMFALAVLALTVSLLLFLREIFLAVSTPRHIPR"
     gene            1446..>2789
                     /locus_tag="VGO02_04565"
     CDS             1446..>2789
                     /locus_tag="VGO02_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEV7476994.1"
                     /translation="MAHSIKRIRTLALVGQAGCGKTSLAEALLAKSGSVPPGGSVERG
                     STVCDYHPLEKQLQHSLKLAVASFESQDTRIHLLDTPGYPDFLGHALPALAAVETAAV
                     VINAQNGIEMMTPRFMQWAAKRGLDRLLIVNKIDADNVDCEALLERIQQVFGKECLPL
                     NLPAGKRSRVSDCFFAPSGEADFSSVDEAHRKLVDQVVEVDEQLMAAYLEKGEVTPEE
                     LHEPLERALREGHLIPVVFTSAKTGAGIAELLEVIVRLLPNPAEGNPPAYISVPAGGG
                     EPADLQPIPDPGRHVLAHVFKIEIDPYIGRLAVFRVHQGRVTPGTQLYIGDGRKPVKA
                     GHLFLLKGKQQTEVSEGLPGDILAVAKIDEIQFDHILHDSASDAQVHARPLELPVPVF
                     GLAVQSKKRGDEQKVSDVLHKIIAEDPCFRIEHNAQANETVMRGLGEIHLKAVLEK"
BASE COUNT          496 a          951 c          893 g          449 t
ORIGIN      
        1 gtcgaacgcg tgtccggggg ttgcgatgcc gagcaatgtt gccagcagcg cgacttgctt
       61 gcttgccatg tttctttctc ctcaccacgc ccaaagaggc gtgcgaagag actaaaaggg
      121 aaggcgctac acggactatc aggggaagcc cgcccggagt ggtccggtgg gcagggattg
      181 cgttacccgc gcatgaagcc gttcaggtcc ggcgtactta tcgtgcgctc gaggtagccg
      241 aagctgccct tgtccttcac ctcgcgcgcc gcctcgacca ggccgctcat cgccgcgcgg
      301 tagagcgagg tggcgacgct gatgcgccgc accccgacgg cctccagctc ggcgacgctg
      361 aacgacttgc ccttgatgcc ggccatgaag ttgaacggct tcatgcgcgg gtaacgcaat
      421 ccctgcccac cggaccactc caggcgggct cccgctggta gtccgtgtag cgccttccct
      481 tttaatctcc cctcacgcct ctttgggcgt ggtgaggaga aagaaatggc aagcaagcaa
      541 ctcgcggtgc tggcaacatt gctcggcatc gcaacccccg gacacgcgtt cgacacccgt
      601 aacgaggccc agacggtgat gttctactac gcgataccgc tcgatgcgca ctcgaagaag
      661 gaccgcgcgc cctggctggg catgcagatc aacggcaagc gcgactacca gagctacagc
      721 atggacagca agctccttac cttcaacttc gaagagagcg gctcggcggc agcgagcgct
      781 ctgatcatcg gcgcggtcgc cgtcggcgca gttgcggttg tcggctcgcg cggcaagagc
      841 tcgcaggagc aggtccagca aaagcaggca tccacgccgc agccgccggc tccggtaccc
      901 tgcccgaccg actgcgggaa gtagcgatgc cctgactccg ggatagactg gcggcttggc
      961 atacgagccg cacatcccgg agatcgccgc cgtcatccag ctgacggtgg cgccggtgtt
     1021 catgctcacg gcagtgggta cgatcatcgg cgcgctcaat atccgcctcg gccgcgccgt
     1081 cgaccggcgc cacgagctgg aggaagagct gacccgcatg ccggccgacg agaagccgtc
     1141 ggcgcaccag gagctcgggt tcatcgcccg gcgcatccgc ttcgtctact actcgatcct
     1201 ggcggcggtg gtctcgggcc tgttcacctg cctgctgatc gccggggcct tcctcgccac
     1261 cttcctccgt tcggacatct cccacaccat agggaccatg ttcgccctgg ccgtgctggc
     1321 ccttacggtc agcctgctgc tgttcctgcg cgagattttt ctcgccgtct cgaccccgcg
     1381 gcatattccc aggtagcgcg cgccggcgta taaatgtctc gtcctcatcg gaggaggagg
     1441 cagccgtggc tcattcgatc aagcgcatca gaacactggc gctcgtcggc caggcggggt
     1501 gcggcaagac ctcgctggcc gaagcgctac tggctaaatc gggatcggta cccccggggg
     1561 gtagcgtgga gcgaggcagc accgtctgcg actaccaccc cctagaaaag cagctgcagc
     1621 actccctcaa gctcgcggtc gcgagcttcg aatcgcagga cacccgaatc cacctcctcg
     1681 acacccccgg ctacccggac ttcctcggcc atgccctgcc ggcgctggcg gcggtcgaaa
     1741 ccgcggccgt ggtgatcaat gcgcagaacg gcatcgagat gatgacgccg cgcttcatgc
     1801 aatgggccgc caagcgcggc ctcgaccgtc tgctgatcgt caacaagatc gacgccgaca
     1861 acgtcgactg cgaggcgctg ctcgagcgca tccagcaggt cttcggcaag gaatgcctgc
     1921 cgctcaatct gccggcggga aagcgcagca gggtgtccga ctgcttcttc gcgccttcgg
     1981 gcgaggcgga cttctcctcg gtcgacgagg cgcatcgcaa gctggtcgac caggtggtcg
     2041 aggtcgacga gcagctgatg gcagcctacc tggagaaggg cgaggtgacg cccgaggagc
     2101 tgcacgagcc gctcgagcgc gcgctgcgcg agggccacct catcccggtg gtgttcacct
     2161 cggcgaaaac cggcgccggc atcgccgagc tgctcgaggt gatcgtaaga ctgctgccca
     2221 acccggccga aggcaacccg cccgcgtaca tcagcgttcc cgccgggggc ggcgagccgg
     2281 ccgacctgca accgattccc gatccgggcc ggcacgtcct cgcgcacgtc ttcaagatcg
     2341 agatcgatcc gtacatcggg cgcctggcgg tgttccgcgt gcaccagggg cgcgtcacgc
     2401 ccggcaccca gctctacatc ggcgacgggc gcaagccggt caaggccggg cacctgttcc
     2461 tcctcaaggg caagcagcag accgaggtaa gcgaggggct gccgggagac atcctcgcgg
     2521 tggcgaagat cgacgagatc cagttcgacc acatcctgca cgactcggct tccgacgcgc
     2581 aggtccacgc ccgcccgctc gagctgccgg tcccggtgtt cggcctggcc gtgcagtcga
     2641 agaagcgcgg cgacgagcag aaggtgtcgg acgtgctgca caagatcatc gccgaggatc
     2701 cctgcttccg catcgagcac aacgcccagg cgaacgagac ggtgatgcgc ggcctgggcg
     2761 agatccacct caaggcggtg ctcgagaag
//
LOCUS       DASYJA010000213         1694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2848018, whole genome shotgun sequence.
ACCESSION   DASYJA010000213 DASYJA010000000
VERSION     DASYJA010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2848018"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VGO02_04570"
     CDS             complement(<1..602)
                     /locus_tag="VGO02_04570"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEV7476995.1"
                     /translation="MATHELHSSPETCHWGYFDSQLKPSLRIKSGDIATIHCVSGSAE
                     ILPGDPFNVLPEHREILGALKPHLGRHILTGPVYVEGAERGDALAVEVLDIKFRTNWG
                     WNAQRPLAGTLPEDFPFYRLTHIPIDSNRGVATLPWGLEIELKPFFGIMGVAPPPEWG
                     QCSSIEPRAFGGNIDNKHFQVGTTVYLPVFAEGGLFSTGDG"
     gene            679..1542
                     /gene="rlmJ"
                     /locus_tag="VGO02_04575"
     CDS             679..1542
                     /gene="rlmJ"
                     /locus_tag="VGO02_04575"
                     /inference="COORDINATES: protein motif:HMM:NF016278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEV7476996.1"
                     /translation="MNYRHGYHAGNFADVLKHTALCELLRLLTAKDKKLFVLDTHAGA
                     GGYDLAGGRARRTGEAEAGIARLLASSHTGMPPAVTRYLAAVAAYDRKFGPAGSGLRR
                     YPGSPRLVRAALRPGDRFIACELQPAEALALKREFAGDRAVEVRQADGYKVLKALLPP
                     VERRGLVLIDPPFETTDEFERLLRALRQGVRRFATGCYALWYPIKDETAVAFAGELAA
                     FKPLTLDLHMDAVEPGKLAACALAVINPPWRFEEAMREALPWIAEHLGPGVRAAISAP
                     PASAPVPASAG"
     gene            complement(1484..>1694)
                     /locus_tag="VGO02_04580"
     CDS             complement(1484..>1694)
                     /locus_tag="VGO02_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7476997.1"
                     /translation="ALGSIIGALYSGVAFGALLGAPVAGYAFDLFGSYTGVMLAGAAL
                     CLASFVITLLMPEPARWLAGRKSRP"
BASE COUNT          262 a          560 c          606 g          266 t
ORIGIN      
        1 ccgtcgccgg tcgagaacag gccgccttcg gcgaataccg gcaggtagac cgtggtgccc
       61 acctggaagt gcttgttgtc gatgttgccg ccgaaggcgc gcggctcgat cgagctgcac
      121 tggccccatt ccggcggcgg cgccacgccc atgatgccga agaacggctt cagctcgatc
      181 tcgagccccc acggcagggt ggcgacaccg cggttggaat cgatggggat gtgggtcagg
      241 cgatagaacg ggaagtcctc gggcagggtg ccggccagcg gccgctgggc gttccagccc
      301 cagttggtgc ggaacttgat gtcgagcacc tcgaccgcca gcgcatcgcc gcgctcggcg
      361 ccttcgacat agaccgggcc ggtcaggatg tggcggccga gatggggctt cagcgcgccc
      421 aggatctcgc gatgctcggg cagcacgttg aacgggtcgc cgggcaggat ctcggccgag
      481 cccgagacgc agtggatggt ggcgatgtct cccgacttga tgcgcagcga cggctttagc
      541 tggctgtcga aatagcccca gtggcaggtc tcgggcgagg aatgaagttc gtgcgtggcc
      601 atggcgtccc cctttattgc ccccgggttg aagcacatcc cgtgcccaca aatccatcgg
      661 tgatgctata ggcgtcgtat gaactatcgc catggctatc acgccggcaa cttcgccgac
      721 gtcctgaagc acacggcgct ctgcgagttg ctgcgcctgc tgacggccaa ggacaagaag
      781 ctgttcgtgc tcgacaccca cgccggcgcc ggtggctacg atctggcggg cgggcgggcg
      841 cggcgcaccg gcgaggccga ggcgggaatc gcgcgcctgc tcgcttcctc gcataccgga
      901 atgccgcccg ccgtcacgcg ttatctggcg gcggtcgcgg cctacgaccg caaattcggt
      961 cccgccggga gcggtctcag gcgctatccg ggttcgccgc ggctggtgcg cgcggcgctg
     1021 cgcccgggcg atcgcttcat cgcctgtgag ctgcagccgg cggaggcgct ggcgctcaag
     1081 cgcgagttcg ccggcgaccg tgccgtcgag gtgcgccagg ccgacggcta caaggtgctg
     1141 aaggcgctgc tgccgcccgt cgaacgccgc ggcctcgtgc tgatcgatcc gcctttcgag
     1201 acgaccgacg agttcgagcg cctgctgcgc gccctgcgcc agggcgtgcg gcgcttcgcg
     1261 accggctgtt acgcgctctg gtatccgatc aaggatgaga ctgcggtcgc gttcgcgggc
     1321 gaactggcgg ctttcaagcc gttgacgctg gacctccaca tggatgcagt cgaaccgggc
     1381 aagctcgcgg cgtgcgcgct cgcggtcatc aatccgccct ggcgtttcga ggaagccatg
     1441 cgcgaggcgc tgccgtggat cgccgagcac ctggggccgg gtgtcagggc cgcgatttcc
     1501 gccccgccag ccagcgcgcc ggttccggca tcagcagggt gatgacgaag gacgccaggc
     1561 agagcgctgc gcccgccagc atgacgccgg tgtaggaacc gaagagatcg aaggcgtagc
     1621 ccgcgaccgg tgcgccaagc agcgcgccga aggccacgcc gctatagagc gcgccgatga
     1681 tcgagccgag cgcg
//
LOCUS       DASYJA010000214         3402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2211228, whole genome shotgun sequence.
ACCESSION   DASYJA010000214 DASYJA010000000
VERSION     DASYJA010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2211228"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /gene="pyrD"
                     /locus_tag="VGO02_04585"
     CDS             complement(<1..594)
                     /gene="pyrD"
                     /locus_tag="VGO02_04585"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017514992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase (quinone)"
                     /protein_id="HEV7476998.1"
                     /translation="MSLYGLARPLLFALDPETAHRFTLSLLDKSFYSKAALACPVQVM
                     GLKFPNPVGLAAGLDKDAAHIDGLARLGFGFLEVGTVTPRPQPGNPRPRLFRLEKQQA
                     LINRFGFNNVGVDEFLRNVGRARWKGVLGINIGKNADTPAQRAVEDYAIGFEKVYASA
                     SYVTVNVSSPNTKNLRDLQQDSQLNALLEKLSALRVRL"
     gene            complement(591..1340)
                     /locus_tag="VGO02_04590"
     CDS             complement(591..1340)
                     /locus_tag="VGO02_04590"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEV7476999.1"
                     /translation="MSRLNDLPHAVLQFYVTAPYPCSYLPDRMARSQVATPSHLINTE
                     LYGDLVKAGFRRSGIFTYRPHCDACRACVPVRIPTAEFSANRSQRRALKQHGALVATS
                     QPLKFRLEHYALYLRYQSKRHSGGGMDNDSRDQYSHFLLQSRVDTRLVEFRENGQLVM
                     VSIVDYLPDGLSSVYTFYEPERQGASFGTYNVLWQIESCRRLGLPYLYLGYWIRESPK
                     MAYKSKFEPIEGFVGGVWRRLGRRQTEQDRA"
     gene            complement(1345..2043)
                     /gene="aat"
                     /locus_tag="VGO02_04595"
     CDS             complement(1345..2043)
                     /gene="aat"
                     /locus_tag="VGO02_04595"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEV7477000.1"
                     /translation="MLTWLGAGNAFPPVEKALKEPNGLLAAGGDLSAARLLEAYRRGI
                     FPWYAGGEPLLWWSPDPRMVLYCAELKIPRSLAKNLRNKGYEVRVDSAFSKVLKHCAE
                     PRKIEKNTWLGKEMQAAYLALHQAGHAHSFETWRQGDLVGGLYGVAVGRMFYGESMFS
                     RATDASKVALVHLVAYLKERGFPLIDCQMKTPLLASLGGREVPRRAFLRQVAGLVNYA
                     EPSGKWTMRTLGAA"
     gene            complement(2037..2735)
                     /locus_tag="VGO02_04600"
     CDS             complement(2037..2735)
                     /locus_tag="VGO02_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEV7477001.1"
                     /translation="MERRDFLKACAAGAAAIGGTPALAADARPHLYQRAVLVDGKGEP
                     LRTKAIPANRNLIFHYPYASTPCFLLNLGKPAKASAQLRTVDNHPYEWQGGVGARRSV
                     VAYSAICAHKLTYPTRDISFISFRAETSARNRHGNVIHCCSEHSQYDPAEGAKVLAGP
                     APQPLAAILLEWDSSKDEVHAVGTLGGELFNEFFAKYEFRLALDGEAAPRRPVQGRAV
                     VVELENYCKQQVKC"
     gene            complement(2739..>3402)
                     /locus_tag="VGO02_04605"
     CDS             complement(2739..>3402)
                     /locus_tag="VGO02_04605"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HEV7477002.1"
                     /translation="GFPHEVNPPHAPWMVMVGACLLWVGWFGFNAGSALAADGTAGMA
                     MLVTHIGAASGAFAWMVCEWLRYEKPSVLGIVTGMVAGLGTITPASGFVGPIGALAIG
                     GAAGIVCFFATNYMKRVLAVDDSLDVFPVHGVGGILGTLLTGVFVSAYFNGIGYPEKV
                     TMGEQMLVQVCGVLAVGAWSGVATFVLLKICDIVVGMRVAGDEETEGLDTVLHNEKGY
                     NL"
BASE COUNT          570 a         1093 c         1170 g          569 t
ORIGIN      
        1 caatcggact ctcagggcgg agagcttctc caatagtgca ttcaattgag aatcctgctg
       61 caggtcgcgc aggttcttcg tgttcggcga agagacgttc acggtcacat agctggccga
      121 ggcgtacacc ttctcgaagc cgatagcgta gtcctcgacc gcgcgctgcg ccggcgtgtc
      181 ggcgttcttg ccgatattga tgccgaggac ccctttccag cgggcgcggc cgacgttgcg
      241 gaggaactcg tcgacgccca cgttgttgaa gccgaatcga ttgatcaatg cctgttgttt
      301 ttccaatctg aacaacctgg gacgaggatt ccccggctga ggccgcggcg tcaccgtgcc
      361 gacctcgagg aagccgaagc ccaggcgcgc gagcccgtcg atgtgcgcgg cgtccttgtc
      421 cagtcccgcg gcaagaccca cggggtttgg gaatttcaat cccatcacct gcaccgggca
      481 cgcgagtgcc gcttttgaat aaaaggattt gtctagaaga gaaagggtaa agcggtgggc
      541 ggtttcggga tcgagggcga aaaggagcgg acgcgcgagc ccgtaaaggc tcaagcccgg
      601 tcctgctcgg tctgccggcg cccgaggcgc cgccagacgc cgccgacgaa gccctcgatc
      661 ggctcgaact tcgacttgta ggccatcttc gggctctcgc ggatccagta gccgaggtag
      721 aggtagggca ggccgaggcg gcggcaggac tcgatctgcc acagcacgtt gtaggtgccg
      781 aagctcgcgc cctggcgctc gggctcgtag aaggtataga ccgacgacag gccgtcgggc
      841 aggtagtcga cgatcgacac catcaccagc tggccgttct cgcggaattc caccaggcgc
      901 gtgtcgaccc ggctctgcag caggaagtgc gagtactggt cgcggctgtc gttgtccatg
      961 ccgccgccgg agtggcgctt ggactggtag cgcagataga gcgcgtagtg ctcgagacgg
     1021 aacttgagcg gctgcgacgt cgcgaccagc gcgccgtgct gcttgagcgc gcggcgctgc
     1081 gagcggttgg cgctgaactc ggcggtcggg atgcgcaccg gcacgcaggc gcggcaggcg
     1141 tcgcagtgcg ggcggtaggt gaagatgccg ctgcggcgga agccggcctt gaccaggtcg
     1201 ccgtagagct cggtgttgat gaggtggctc ggcgtggcca cctgcgaacg ggccatgcgg
     1261 tccggcaggt agctgcacgg gtaaggcgca gttacgtaga actggagtac ggcgtgcggc
     1321 aggtcgttaa gtcgagacaa gtctttacgc ggctcctagc gtcctcatgg tccactttcc
     1381 ggaaggctcc gcgtagttta ccaaccccgc gacctggcgc aaaaaggcgc gccgcgggac
     1441 ctcgcggccg ccgagcgagg cgagcagcgg ggtcttcatc tggcagtcga tcaggggaaa
     1501 gcccctttcc ttcaaatagg cgaccaggtg caccagcgcc accttcgacg cgtcggtggc
     1561 gcgcgaaaac atcgattctc cgtagaacat gcgccccacc gccaccccgt agagccctcc
     1621 gaccagatca ccctggcgcc aggtttcgaa ggaatgggcg tggccggcct ggtgcagggc
     1681 caggtaggcg gcctgcatct cctttcccag ccaggtgttt ttctcgattt ttctcggttc
     1741 tgcacagtgt tttagtactt tggaaaaagc gctgtccacc cgcacctcgt agcccttgtt
     1801 gcgcaggttt ttcgccagcg agcgcgggat cttgagctcc gcgcagtaca gcaccatgcg
     1861 ggggtccggc gaccaccaga gcagcggctc gccgccggcg taccagggga agatgccgcg
     1921 gcggtaggcc tccagcagcc gcgccgccga caggtcgccg cctgcggcga gcagcccgtt
     1981 cggctccttg agggctttct cgaccggcgg gaaggcgttg ccggcgccga gccaggtcag
     2041 cacttcactt gctgtttgca gtagttctcg agctcgacca ccaccgcgcg gccctgcacc
     2101 gggcggcgcg gcgcggcttc gccgtccagc gccaggcgga actcgtactt ggcgaagaac
     2161 tcgttgaaca gctcgccgcc gagggtgccc accgcatgca cctcgtcttt cgaggaatcc
     2221 cattcgagca ggatcgcggc gagcggctgc ggcgcggggc cggcgagcac cttggcgccc
     2281 tcggcggggt cgtactggct gtgctccgag cagcagtgga tgacgttgcc atggcggttg
     2341 cgcgcgctgg tctcggcgcg aaagctgatg aagctgatgt cgcgcgtcgg gtaggtgagc
     2401 ttgtgcgcgc agatcgcgga gtacgcgacg accgagcgcc gcgcgccgac gccgccttgc
     2461 cactcgtagg gatggttatc gacggtcctg agctgcgccg atgccttggc cggcttgccg
     2521 aggttcagca ggaagcaggg cgtcgaggcg tacgggtagt ggaagatcag gttacggttg
     2581 gccgggatcg ccttcgtgcg cagcggctcg cccttgccgt cgaccagcac cgcgcgctgg
     2641 taaagatgcg gccgggcgtc ggcggcgagg gccggcgtgc cgccgatggc cgcggcgccg
     2701 gcggcgcacg ccttcaggaa atcacggcgc tccatgcctc acaggttata acctttttcg
     2761 ttgtgcagca ccgtgtcgag gccctcggtt tcctcgtcgc cggcaacgcg catgcccacc
     2821 acgatgtcgc agatcttgag cagcacgaag gtggccacgc ccgaccaggc gccgacggcg
     2881 agcacgccgc atacctgcac cagcatctgc tcgcccatcg tcactttctc ggggtagccg
     2941 atgccgttga agtacgccga gacgaacacc ccggtgagca gcgtgccgag gatgccgcct
     3001 acgccgtgca ccgggaagac gtcgagcgag tcgtcgacgg ccagcacgcg cttcatgtag
     3061 ttggtggcga agaagcagac gatgcccgcc gcgccgccga tggcgagcgc gccgatcggg
     3121 ccgacgaagc ccgaggccgg cgtgatggtg cccaggcccg ccaccatgcc ggtgacgatg
     3181 cccagcaccg agggcttctc gtagcgcagc cactcgcaca ccatccaggc gaacgcgccg
     3241 ctggcggcgc cgatatgggt gaccagcatg gccattccgg ccgtgccgtc cgcggcgagc
     3301 gcgctgccgg cgttgaagcc gaaccagccc acccacagga ggcatgcgcc gaccatcacc
     3361 atccacgggg cgtgcggcgg gttcacctca tgcgggaagc cg
//
LOCUS       DASYJA010000215         4158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1485874, whole genome shotgun sequence.
ACCESSION   DASYJA010000215 DASYJA010000000
VERSION     DASYJA010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485874"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1405)
                     /locus_tag="VGO02_04610"
     CDS             complement(95..1405)
                     /locus_tag="VGO02_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEV7477003.1"
                     /translation="MRFLPARRLLWLVTAMAAAALGVAIAGAFQPQWLTLAAALVLVA
                     GLDLLSGLRLPPPAVARRLPATLALGVRIDVALRVANLSGRRLRCELHDHHPASFEGE
                     GLPRSLSLAPLEWTEVVYQVRPVARGATRFGATELRLFSPLGLWQITRNVGNQSEVRV
                     YPNFRTLAKYTLLATDNRLSQIGVLQVRRRGEGMEFHQLREYRQGDSQRAIDWKATSR
                     TARLIAREYEDEKDQRVLLVVDCGRRMASKDDELSHFDHALNSALLLAHVAARQGDAV
                     GMLTMAGVSRYCEPRKSIAAVNAILNQAYDIEPTLAVPDYHQAARDVMRHVRRRALVI
                     VLTNLRDEDDSTLLPALKLLRTRHLVVLASLREAILSRALSARVDSFDRAVTHAAAAG
                     YLAERERSFRRLDAAGVITLDVEPERLAISLVNRYLELKRAGRL"
     gene            complement(1411..2397)
                     /locus_tag="VGO02_04615"
     CDS             complement(1411..2397)
                     /locus_tag="VGO02_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEV7477004.1"
                     /translation="MNTQAKPTAEQFAQATKVAQAMRAEVGKALVGQADVVDHVLCAL
                     LAGGHVLVEGVPGLGKTLLVRALARTISGSFGRVQFTPDLMPADVTGHTLYDPKNQVF
                     TTRRGPVFVNLLLADEINRAPAKTQAALLEVMQEGQVTIEGEALLLEPPFMVLATQNP
                     IEQEGTYALPEAQLDRFLLKVKIDYPSQAEEAAMVRQVTDGKIGDRLNVEQVGTLLKP
                     PTVVMLQTITAALRVDEAVLDYAIRLVRATRTWAGVIAGAGPRGGISLIRAARGAALL
                     AGRDFVTPDDVKRMALPALRHRIAPSPELELEGRDADGILKGLLEKIEAPRK"
     gene            complement(2394..3509)
                     /locus_tag="VGO02_04620"
     CDS             complement(2394..3509)
                     /locus_tag="VGO02_04620"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEV7477005.1"
                     /translation="MRSRVLVIGGLGLLAVLGAVWFLSTYERVPYKEQVGPSSEVRLR
                     QFLAAERFAERMGVPASEIRSLPELEALEPGGVLLMPARRQALEPRRLRDIAAWVERG
                     GHLVAEAEFLGVSDPLFDLLGVRRDQVDATGKPLIVEVDGRKLTVSLASRMTLQAPAG
                     ARVELRAGPAEAARLISYHRGKGMVTAVTSLQFVRNYQIGANDHAELFWELLSLTPAP
                     ALQVYHRPERLSLTGFLRERAPEAAGACLALLALWLWRIAPRFGPVAPDAPPARRRLL
                     DHLRASGRYYWAKGLRGELVLAARDAALRRLARAQPDFAVASQPEKVSRLASLTGISQ
                     EEARRFLAAGGPMRGADFIKLARDAQSVHTALEKGKK"
     gene            complement(3506..>4158)
                     /locus_tag="VGO02_04625"
     CDS             complement(3506..>4158)
                     /locus_tag="VGO02_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477006.1"
                     /translation="FSVFCFLALPTNSHAQKAPKKEIAEVLKAKEFGYERDATRWQSR
                     DPEKPRDKPDSDPRAFWGIGYALARAAEVLLWGLAAAAAAYALWFLARMLPRGRASAP
                     EPYRPPPALFGMELAPETLPPDVAAAAAALAREGKLREALSLLYRGSLSNLVHQRGVQ
                     LLPSHTEAEVLSLSGQEVHSYLEKLIAIWRLCAYAKRDPAPADIEQLAQGYQASFA"
BASE COUNT          593 a         1445 c         1526 g          594 t
ORIGIN      
        1 tggttcgagc tggtgtcgag gaagatcacc ggccggtatt cggggtggat gcctgatttc
       61 atagggcgcg aattataaag gaaaaagcct gtttttacag ccgtcccgcc cgcttcagct
      121 ccaggtagcg gttgaccagc gagatggcca ggcgctcggg ctccacgtcc agggtgatca
      181 ccccggcggc gtccaggcgc cggaagctgc gctcgcgctc ggccaggtag ccggcggcgg
      241 cggcgtgggt cactgcgcgg tcgaagctgt cgacccgggc cgacagcgcc cgcgacagga
      301 tggcctcgcg cagcgaggcg aggaccacca ggtgccgggt gcgcaggagc ttcagcgccg
      361 gcagcagggt gctgtcgtcc tcgtcgcgca ggttggtgag cacgatcacc agcgcgcggc
      421 gccgcacgtg gcgcatcacg tcgcgcgcgg cctggtggta gtcgggcacc gcgagggtcg
      481 gctcgatgtc gtaggcctgg ttgaggatgg cgttcaccgc cgcgatcgat ttgcgcggct
      541 cgcaatagcg gctcaccccg gccatcgtca gcatgccgac cgcgtcgccc tggcgtgccg
      601 cgacgtgggc aagcagcagc gcggaattga gggcgtggtc gaagtgcgac agctcatcgt
      661 ccttcgacgc catgcgccgg ccgcagtcga ccaccagcag cacgcgctgg tccttctcgt
      721 cctcgtattc gcgcgcgatg aggcgcgcgg tgcgcgaggt cgccttccag tcgatggcgc
      781 gctgcgagtc gccctggcgg tactcgcgca gctggtggaa ctccatgccc tcgccgcggc
      841 ggcgcacctg cagcacgccg atctgcgaca ggcggttgtc ggtggcgagc agggtgtatt
      901 tcgccagcgt gcggaagttg ggatagaccc gcacttcgct ctggttgccc acgtttcttg
      961 ttatctgcca caagcccaaa ggcgagaaga gccgcagctc ggtcgcgccg aagcgcgtcg
     1021 cgccgcgcgc caccgggcgc acctggtaca cgacctcggt ccattcgagc ggcgcgagcg
     1081 agaggctgcg cggcaggcct tcgccctcga agctcgccgg atggtggtcg tgcagctcgc
     1141 agcgcagccg ccggcccgag aggttggcga cgcgcagcgc gacgtcgatg cgcacgccga
     1201 gggcgagcgt cgccggcagg cggcgcgcga cggccggcgg cggcagccgc agcccggaga
     1261 gcaggtcgag ccccgccacc agcacgaggg cggcggcgag cgtgagccac tgcggctgga
     1321 aggcgccggc gatcgccacg ccgagcgcgg cggccgccat cgcggtgacc agccacagca
     1381 ggcgccgggc gggcaggaag cgcatgacgg ctatttcctt ggagcctcga tcttctccag
     1441 cagccctttg aggatgccgt cggcgtcgcg gccttccagc tcgagctcgg gcgagggcgc
     1501 gatgcggtgg cgcagcgcgg gcagcgccat gcgcttcacg tcgtcggggg tgacgaagtc
     1561 ccggcccgcg aggagcgcag cgcctcgcgc tgccctgatc agcgagatgc cgccgcgcgg
     1621 cccggcgccg gcgatcacgc ccgcccaggt gcgcgtcgcg cgcaccaggc ggatggcgta
     1681 gtcgagcacc gcctcgtcca cgcgcagcgc cgcggtgatg gtctgcagca tcaccaccgt
     1741 cggcggcttg agcagcgtgc ccacctgctc gacgttgagc cggtcgccga tcttgccgtc
     1801 ggtcacctgg cgcaccatgg ccgcctcctc ggcctggctg gggtagtcga tcttcacctt
     1861 gaggaggaag cggtcgagct gcgcctcggg cagcgcgtag gtgccttcct gctcgatcgg
     1921 gttctgcgtc gcgagcacca tgaagggcgg ctcgaggagc agagcctcgc cctcgatcgt
     1981 cacctggccc tcctgcatca cttccagcag cgccgcctgg gtcttggccg gggcgcggtt
     2041 gatctcgtcg gccagcagca ggttgacgaa caccggcccg cggcgcgtgg tgaacacctg
     2101 gttcttcggg tcgtacagcg tgtgcccggt gacgtcggcc ggcatcaggt cgggcgtgaa
     2161 ctgcactcgc ccgaagctgc ccgaaatcgt gcgggccaag gcccgtacga gcagcgtctt
     2221 gcccaggccg ggcacgcctt cgaccagcac gtggccgccg gcgagcaggg cgcacagcac
     2281 gtggtcgacg acgtcggcct ggccgaccag cgccttgccg acctcggcgc gcatggcctg
     2341 cgccaccttg gtcgcctggg cgaactgctc ggcggtgggt tttgcctggg tgttcatttt
     2401 tttcctttct ccaaagcggt gtgcacgctc tgcgcgtcgc gcgcgagctt gatgaaatcg
     2461 gcgccgcgca tcgggccgcc ggcagcgagg aagcggcgcg cttcttcctg ggaaataccc
     2521 gtcaatgacg ccagacggga aaccttttcc ggttgcgagg cgacggcgaa gtcgggctgc
     2581 gcgcgggcga ggcggcgcag cgccgcgtcc cgcgcggcga gcaccagctc gccgcgcagg
     2641 cctttcgccc agtagtagcg gccgctcgcg cgcaggtggt cgagcaggcg ccggcgcgcc
     2701 ggcggcgcgt cgggcgcgac cgggccgaag cgcggcgcga tgcgccacag ccacagcgcg
     2761 agcagcgcga ggcacgcgcc ggccgcctcg ggcgcgcgct ccctgagaaa gccggtgagc
     2821 gagagccgct cgggccggtg gtacacctgc agcgccggcg cgggcgtgag gctaagcagc
     2881 tcccagaaga gctcggcgtg atcgttcgcg ccgatctggt agttgcgcac gaactgcagg
     2941 ctggtcactg ccgtcaccat ccccttgccg cgatggtagg aaatcagccg cgcggcttcg
     3001 gcgggcccgg cgcgcagctc gacgcgcgcg ccggccggcg cctgcagcgt catgcggctg
     3061 gcgagcgaca ccgtgagctt gcggccgtcg acctcgacga tgagcggctt gccggtggcg
     3121 tcgacctggt cgcgccgcac ccccagcagg tcgaacagcg ggtcggagac gccgaggaat
     3181 tccgcctccg cgacgaggtg gccgccgcgc tcgacccagg cggcgatgtc gcgcaggcgg
     3241 cgcggctcga gcgcctggcg gcgcgccggc atgagcagca cgccgccggg ctcgagcgcc
     3301 tcgagctcgg gcagcgagcg gatctcgctc gctggcacgc ccattcgctc ggcgaagcgc
     3361 tcggcggcga ggaactggcg cagccgcacc tcgctcgacg ggccgacctg ctccttgtac
     3421 ggcacgcgct cgtaggtcga gaggaaccac accgcgccga gcaccgcgag caggcccagg
     3481 ccgccgatga cgaggacgcg gctcctcatg cgaaggacgc ctggtaaccc tgcgcgagct
     3541 gctcgatgtc cgcgggcgcc gggtcgcgct tcgcgtaggc gcaaagccgc cagatggcga
     3601 tcagcttttc gaggtaggaa tggacctcct gcccggaaag cgaaagcacc tccgcctcgg
     3661 tatggctcgg caggagctgc acgccgcgct ggtgcaccag gttcgagagc gagccgcggt
     3721 agagcaggct cagcgcctcg cgcagctttc cctcgcgggc cagcgccgcg gcggccgccg
     3781 cgacgtcggg cggcagcgtt tccggcgcga gctccatgcc gaacagcgcc ggcggcggcc
     3841 ggtacggctc gggcgcgctc gcgcggccgc gcggcagcat gcgcgcgagg aaccacaggg
     3901 cgtaggcggc cgcggcggcc gccagccccc acagcagcac ctcggccgcc ctggcgagcg
     3961 catagccgat gccccagaac gcgcgcgggt cggagtccgg cttgtcgcgc ggcttttccg
     4021 ggtcgcgcga ctgccagcgc gtcgcgtcgc gctcgtagcc gaattccttg gctttcagca
     4081 cctcggcgat ttctttcttg ggagctttct gcgcatggga attcgtcggc agcgcgagaa
     4141 aacaaaaaac cgaaaaaa
//
LOCUS       DASYJA010000216         3036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2392619, whole genome shotgun sequence.
ACCESSION   DASYJA010000216 DASYJA010000000
VERSION     DASYJA010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2392619"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /gene="fabG"
                     /locus_tag="VGO02_04630"
     CDS             complement(<1..719)
                     /gene="fabG"
                     /locus_tag="VGO02_04630"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEV7477007.1"
                     /translation="MKRALVTGGSGGIGAAICGQLAADGLQVIVHANSRLAEAERIAA
                     GIRAGGGAAQAAAFDVTDAARTAQALQELLGGGPVQVLVNNAGIHDDAVMPAMKREQW
                     SRVIDVSLNGFFNVTQPLLLPMMATRWGRVINVSSVAALTGNRGQANYAAAKAGLHGA
                     TKSLAIELASRGITVNAVAPGIIASAMTDGLFDQETIARLVPMKRLGTPAEVAALVAF
                     LASEQAGYISGQIISVNGALA"
     gene            complement(716..1132)
                     /locus_tag="VGO02_04635"
     CDS             complement(716..1132)
                     /locus_tag="VGO02_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxylacyl-ACP dehydratase"
                     /protein_id="HEV7477008.1"
                     /translation="MILDRGAIARLIPHGGAMCLLHEVLEVDAESIRARAVSHRDPAH
                     PLREADTLPALCGIEYAAQAMAVHGAMQEGRREHGMLAALRDVELAVQRLDDIAQDLI
                     VEAHCLLNESGRLLYQFQIKAGSRELLRGRATVVTA"
     gene            complement(1129..1911)
                     /locus_tag="VGO02_04640"
     CDS             complement(1129..1911)
                     /locus_tag="VGO02_04640"
                     /inference="COORDINATES: protein motif:HMM:NF025100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HEV7477009.1"
                     /translation="MIGLSVRGVGILAPGLPDWATARRVLAGEQAYHHAPAAEPKPQA
                     LPPNERRRGARTVRWAIAAAQEALRDSGIAAADAASVFASSSGDGEVLVQILESLVQP
                     AREVSPTRFHNSVHNAASGYWSIASGSRRAAVTLCAYDACFGAALLEAAALVRAGEGA
                     VLLVAYDLPYPPPFLAVRPIREPLALALLLCAETGAPALARWRVALAAEPATIAAMPI
                     PELESNPAAHALPLLAAMARNETIEIQVSLANGGALTIETRP"
     gene            complement(1908..>3036)
                     /locus_tag="VGO02_04645"
     CDS             complement(1908..>3036)
                     /locus_tag="VGO02_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEV7477010.1"
                     /translation="CAATLEALRSRRGGLRPNDLEGVALDTWIGRVAGVEDAPLGAGL
                     AAFDCRNNRLAALGLRADGFEAAVARARGRYGAERIALLLGTTTSGIAESERAFAQRG
                     TAGELPAWFRYRETHNLFSVCDFVQRQLGLRGPATTISTACSSSAKVFASAQRLVEAG
                     LADAAVVGGVDSLCLMTLYGFNSLQLLSSTPCRPCDAARDGLSIGEAAGFALLEPARP
                     GAGLALLGYGESSDAYHMSSPHPDGEGAAAAMRAALERAGAEPAQVDYVNLHGTGTPA
                     NDRAEDAAAFGLFGAEVACSSTKGWTGHALGAAGIVEAVISLLCLRHGLIPGSLNLES
                     VDPALKSRIAVAPEERPLARVLSNSFGFGGNNCSLLFGTLA"
BASE COUNT          424 a         1077 c         1118 g          417 t
ORIGIN      
        1 caggccaacg cgccgttgac cgagatgatc tgcccggaga tgtagccggc ctgctcggaa
       61 gccaggaatg ccaccagcgc ggccacctcc gccggcgtgc cgaggcgttt catgggcacc
      121 agccgcgcga tggtctcctg gtcgaaaaga ccgtcggtca tggcgctggc gatgatgccg
      181 ggcgcgaccg cgtttacggt aatcccgcgg ctggcaagct cgatggcgag ggatttcgtc
      241 gcgccgtgca ggcccgcctt ggctgcggcg tagttggcct gcccgcggtt gccggtcaga
      301 gcggcgacgg aggagacgtt gatgacgcgc ccccagcggg tggccatcat cggcagcagc
      361 agcggctgcg tcacgttgaa gaatccattc agggaaacgt cgatcacgcg cgaccattgc
      421 tcgcgcttca tggccggcat gaccgcgtcg tcgtggattc cggcattgtt caccagcacc
      481 tggaccggac cgccgcccag caattcctgc agcgcttgcg ctgtgcgcgc cgcgtcggtc
      541 acgtcgaacg ccgcggcctg cgcagcgccg ccgccggcac ggatgccggc cgcgatgcgc
      601 tctgcttccg ccaggcggct gttggcgtgc acgatgacct gcaggccgtc ggcggcgagc
      661 tgcccgcaga ttgccgcgcc gatgccgccg ctgccgccgg tgaccagcgc ccttttcatg
      721 ccgtcaccac ggtggcgcgg ccgcgcagca gttcgcgact tcccgccttg atctggaatt
      781 gatagagcag gcggccgctt tcgttcagca ggcagtgggc ttccacgatc aggtcttgcg
      841 cgatgtcgtc gaggcgctgc accgcgagtt ccacgtcgcg cagcgccgcc agcatgccgt
      901 gctccctcct gccttcctgc attgcgccat gcaccgccat cgcctgcgct gcgtactcga
      961 tgccgcacag cgcgggcaac gtgtccgcct cgcgcagcgg atgcgccggg tcgcggtggc
     1021 tcacggcgcg cgcgcggatg ctctcggcgt cgacctcgag cacctcgtgc agcaggcaca
     1081 tcgcgccgcc gtgcgggatg aggcgcgcga tggcgccgcg gtcgaggatc acggccgcgt
     1141 ctcgattgtc agcgcgccgc cgttcgcgag gctgacctgg atttcaatcg tttcattgcg
     1201 cgccatcgcg gcgagcaggg gtaaagcatg ggcggcggga ttggactcca gctccggaat
     1261 cggcatcgcg gcgattgtcg caggttcggc ggcgagcgcg acccgccagc gggcgagggc
     1321 cggcgcgccg gtctcggcgc acagcagcag agcgagcgcg agcggctcgc ggatcggccg
     1381 taccgcaaga aacggcggcg ggtagggaag atcgtaggcg accagcagca ccgcgccttc
     1441 gccggcccgc accagcgccg cggcctcgag cagcgccgcg ccgaagcagg cgtcgtaggc
     1501 gcacagggtc acggcggcgc ggcgcgaccc cgaggcgatg ctccagtatc ccgaggcggc
     1561 gttgtgcacc gagttgtgga agcgggtggg cgagacctcg cgcgccggct gcaccagcga
     1621 ctcgaggatc tgcaccagga cctcgccgtc gcccgagctc gaggcgaaga ccgaggcggc
     1681 gtccgcggcg gcgatgccgc tgtcgcgcag cgcctcctgc gccgccgcga tcgcccagcg
     1741 cacggtgcgc gcgccgcgtc gccgctcgtt cggcggcagg gcttgcggct tgggctcggc
     1801 tgccggcgcg tggtggtagg cctgctcgcc ggcgagcacg cggcgcgccg tagcccagtc
     1861 cggcaggccg ggcgcgagga tgccgacacc gcgcacggac aggccgatca agcgagggtt
     1921 ccgaacagca ggctgcagtt gttgccgcca aagccgaagg aattcgagag cacgcgcgcg
     1981 agcggccgct cctcgggcgc gacggcgatg cggcttttga gggccggatc gacgctctcg
     2041 aggttgaggc tgccggggat gaggccatgc cgcaggcaca gcagcgagat caccgcctcg
     2101 acgatgcccg ccgcgccgag cgcgtgcccg gtccagccct tcgtggagct gcaggcgacc
     2161 tcggcgccga acaggccgaa ggccgcagcg tcctcggcgc ggtcgtttgc cggcgtgccg
     2221 gtgccgtgca ggttgacgta gtccacttgc gcaggctcgg cgccggcgcg ctcgagcgcc
     2281 gcgcgcatgg cggcagccgc gccctcgccg tcggggtgcg gcgaggacat gtggtacgcg
     2341 tcggagctct cgccgtagcc gagcagcgca aggccggcgc ccggccttgc gggctccagc
     2401 agcgcgaatc ccgcggcctc gccgatcgac aggccgtcgc gcgccgcgtc gcagggccgg
     2461 cacggcgtgc tcgacaaaag ctgcagcgag ttgaacccat acagtgtcat caagcacagg
     2521 ctgtcgacgc cgcccactac cgcggcgtcg gcgagccccg cttcgaccag gcgctgggcg
     2581 ctggcgaaga ccttggcgct ggaggagcag gcggtggaaa tcgtcgtcgc cggcccgcgc
     2641 aggccgagct ggcgctgcac gaagtcgcac accgagaaca ggttgtgcgt ctcccggtag
     2701 cggaaccaag ccggcagctc gccggcggtg ccgcgctgcg cgaaagcgcg ctcgctctct
     2761 gcgatgcccg aagtggtggt gccgagcagc agcgcgatgc gctctgcgcc gtagcgaccg
     2821 cgcgcgcggg ccaccgcggc ctcgaagccg tcggcgcgca ggccgagcgc cgccagacgg
     2881 ttgttgcggc agtcgaacgc cgcgagcccg gcgccgagcg gcgcatcctc cacgccggcg
     2941 acgcggccga tccacgtgtc gagcgcgacc ccttccaggt cgttcggccg caggccgccg
     3001 cggcggctgc gcagcgcctc gagtgtcgcg gcgcag
//
LOCUS       DASYJA010000217         5449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2666153, whole genome shotgun sequence.
ACCESSION   DASYJA010000217 DASYJA010000000
VERSION     DASYJA010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2666153"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VGO02_04650"
     CDS             complement(<1..663)
                     /locus_tag="VGO02_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477011.1"
                     /translation="MPRAAFYVLFTASGFAGLIYESIWTHYLKLFLGHAAYAQSLVLV
                     VFMGGMALGAGLCGRLSERLANPLLVYAAVEAAVGVLALVFHPVFVGVTDWGYGSLLP
                     WLGNEWLALGGKLALSCLLILPQSVLLGATFPLMSAGLVRAQPAAAGESVAMLYFSNS
                     LGAAAGVLASGFLLIAWVGLPGTLRTAGVLNLLLAVVVWLLCRPLHLSPVRGDSQAEN
                     ASL"
     gene            691..1815
                     /locus_tag="VGO02_04655"
     CDS             691..1815
                     /locus_tag="VGO02_04655"
                     /inference="COORDINATES: protein motif:HMM:NF037955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7477012.1"
                     /translation="MPLSFRLALFYFMFFLYAGAYVAYFPAYLAARGLGAAQIAWVIA
                     LPAFGRMIAPTAWGALADRTGWQRGIVVFACVANAACFTLLPYMPGFGAIAWLIAGTS
                     ALAAAALPLVEAITLGALAERPGRYGPIRLWGSIGFIAAVLGGGAWLDVRSVESLPAA
                     LMVCAAACVAVALCLPAGAKHAAQKAGPLVIGRPAQALLAAGFCMSAAHGALYAFFTL
                     HLQREGYSGSAIGLYWTIGVLAEIVVFLYLPTLFRRFALSTILVASFACAVVRFLAIG
                     WGAALPAILLPAQLLHAATFGAFHAASVAAVHRVFPPEAQGRGQTLFSSICYGAGGAV
                     GALLAGWAWETAGPGLAFSVSALVGLAGLLLAYPLKRVGL"
     gene            1856..2671
                     /locus_tag="VGO02_04660"
     CDS             1856..2671
                     /locus_tag="VGO02_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEV7477013.1"
                     /translation="MTRKLLIALSVAAAFATGCQTVETTQSGVVGVDRQQHMLISSEQ
                     MNAGAEKAYADLLAQAKAKGALDSDPAQAQRVQAIVKRLIPQTAVFRKDAPGWKWEAH
                     VIGTKDVNAFCMPGGKMAVFSGLIQSLNATDDELAAVMGHEIAHALREHSREQASQQA
                     AQGIGLSVIGALAGLGDAGMSLSQMAMDVTFNLPHSRQDEQEADRIGVELAARAGYDP
                     RAAIALWEKMQKLGGGQPPQILSTHPSNESRIADLKQYAEKVMPLYQQATAKR"
     gene            2709..4124
                     /gene="leuC"
                     /locus_tag="VGO02_04665"
     CDS             2709..4124
                     /gene="leuC"
                     /locus_tag="VGO02_04665"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEV7477014.1"
                     /translation="MGQTLYDKLWDSHVVHAEDDGTALLYIDRHLVHEVTSPQAYEGL
                     KLAGRKPWRIGSVVATADHNTPTRGWERGVEGIDDRFAKLQIQTLDANMKEYGAKVYF
                     PFLDKRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGCLAHGIGTSEVEHVLATQ
                     CLTTRKMKNMRVSVDGPLGRGVTAKDVVLAIIGRIGTAGGTGCAIEFAGSTIRALSME
                     GRMTVCNMAIEAGARAGMIAVDDVTIDYVKGRPFSPSGPTWDAAATYWRTLKSDPDAK
                     FDREVKLDAREIKPQVTWGTSPEMVVTVDDAVPDPEKEKDSGRREGMERALVYMGLKP
                     RTRITDIKLDKIFIGSCTNSRIEDLRVAAAVVKGRRVASNIRQAMVVPGSGLVKTQAE
                     KEGLDKVFRDAGFEWREPGCSMCLGMNDDRLGAGERCASTSNRNFEGRQGEGGRTHLV
                     SPAMAAAAAIAGHFVDIRTWR"
     gene            4124..4717
                     /gene="leuD"
                     /locus_tag="VGO02_04670"
     CDS             4124..4717
                     /gene="leuD"
                     /locus_tag="VGO02_04670"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEV7477015.1"
                     /translation="MEKFTILNGLVAPLDRINVDTDQIIPKEHLKSIKRTGFADALFA
                     AWRHDPQFVLNRPQYKGASILLARKNFGCGSSREHAPWALKDYGFRCIIAPSFADIFF
                     TNCFKNGLLPVALREDEVDRLFYEAAAFPGFKLVVNLEQQSVATTDSAQVMRFDVDPF
                     RKYTLINGLDEIGLTLRHTEKIRAFEARRQTQFPWYF"
     gene            4719..>5449
                     /gene="leuB"
                     /locus_tag="VGO02_04675"
     CDS             4719..>5449
                     /gene="leuB"
                     /locus_tag="VGO02_04675"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEV7477016.1"
                     /translation="MKVAVLPGDGIGPEIISQALMVLKKLQLGIQFQEAPVGGAAYAA
                     HGDPLPEKTLSLAKQADAVLFGAVGDPKYDSLPRAKRPEQAILGLRKELGLFANLRPA
                     QVFAELAAASSLKPEVVAGLDILIVRELTGDIYFGQPKGIRDKGGEREGFDTMLYRES
                     EVRRIARVGFEAARKRAKKLCSVDKANVLETSQLWREVVTEESKNYGDVQLSHMYVDN
                     CAMQLVRNPKQFDVIVTGNLFGDILS"
BASE COUNT          951 a         1821 c         1812 g          865 t
ORIGIN      
        1 gaggcttgcg ttttcggctt gagaatcacc tctcacagga gaaagatgaa gcggcctgca
       61 aagcaaccag accacgaccg cgagaagcag attcaagaca ccggccgtgc gcagcgttcc
      121 tggcaatccc acccacgcga tgagcaagaa gccgctcgcc agcacgccgg cggcggcgcc
      181 caggctgttg ctgaagtaca gcatcgccac cgattcgccc gcggccgccg gctgcgcgcg
      241 caccaggccc gcgctcatca gcgggaaggt ggcgccgagc aggaccgact gcggcaggat
      301 cagcaggcag gagagcgcaa gcttgccgcc gagcgcgagc cactcgttgc ccagccacgg
      361 cagcagcgat ccgtatcccc agtccgtcac cccgacaaac accggatgga agacgagggc
      421 cagcacgccc accgccgcct cgaccgccgc atagacgagc aggggattcg ccaggcgctc
      481 cgacaggcgc ccgcaaagcc cggcgccgag ggccatgccg cccatgaaaa cgaccagcac
      541 cagcgactgg gcgtaggcgg cgtggccgag gaacagcttc aggtaatgcg tccagatcga
      601 ctcgtagatc aggccggcga agcccgaggc ggtgaacagg acgtagaacg cggcgcgcgg
      661 cattggcggg cggatgctaa gttaattgca atgcccctgt catttcggct tgcgctcttc
      721 tacttcatgt tttttctcta cgcgggagcc tatgtcgcgt actttcccgc gtacctcgcc
      781 gcgcgcggcc tgggcgctgc gcagatcgcg tgggtgatcg cgctgcctgc cttcgggcgc
      841 atgatcgcgc cgaccgcctg gggcgcgctc gccgaccgca cgggatggca gcgcggcatc
      901 gtggtattcg cgtgcgttgc caacgcggcg tgctttacgc tgttgcctta catgccgggt
      961 tttggcgcca tcgcctggct gatcgctgga accagcgcat tggccgcggc cgctcttccg
     1021 ctcgtggagg ccatcacgct cggcgcgctc gccgagcgtc cggggcgcta cggcccgatc
     1081 cggctgtggg gatcgatcgg cttcatcgcc gccgtgctcg gcggcggcgc ctggctcgat
     1141 gtgcgttccg tggaatcgct gccggcggcg ctcatggtgt gcgctgcggc ttgcgttgcc
     1201 gtggcgctgt gcctgcccgc aggcgcaaaa cacgcggcgc agaaagcggg gccgctggtc
     1261 ataggccggc cggcgcaggc gctgctggcg gcgggcttct gcatgtcggc ggcgcatggc
     1321 gcgctgtacg cattttttac ccttcacctg cagcgcgagg gctacagtgg ctcggcgatc
     1381 ggcctgtact ggacgatcgg cgtgctcgcg gagatcgtgg tgttcctgta cctgccgacg
     1441 cttttccggc gcttcgcgct ttccaccatc ctggtggcga gcttcgcctg cgcggtggtg
     1501 cgcttcctcg ccatcggctg gggggctgcg ctgcccgcga tcctgctgcc cgcccagttg
     1561 ctgcatgccg cgaccttcgg cgcctttcat gccgcttccg tcgcggcggt ccaccgcgtg
     1621 tttccacccg aggcgcaggg ccgcggtcag accctcttct cgagcatctg ctacggcgcc
     1681 ggcggcgcgg tcggcgcatt gctggctggc tgggcgtggg agaccgcggg accgggcctc
     1741 gccttcagcg tgtccgcgct ggtcgggctg gcaggactcc ttcttgccta tcccctgaaa
     1801 cgggtggggc tttaaagtgt caacagggga ggctcgtaac gcgttggaga accgcatgac
     1861 ccgcaaattg ctcatcgccc tttccgttgc cgcggctttc gccaccggct gccagaccgt
     1921 cgaaaccacc cagagcggag ttgtcggcgt ggaccgacag cagcacatgc tgatctcgtc
     1981 cgagcagatg aacgccggcg ccgagaaagc ctacgcggac ctgctcgcgc aagccaaggc
     2041 gaagggcgcg ctcgacagcg acccggcgca ggctcagcgc gtacaggcga tcgtcaagcg
     2101 cctgattccg caaaccgcgg tcttccgcaa agacgcgccg ggctggaaat gggaagccca
     2161 cgtcatcggc accaaggacg tcaatgcgtt ctgcatgcca ggcggaaaga tggccgtctt
     2221 cagcgggctg attcagagcc tcaatgcgac cgacgacgag ctcgccgcgg tcatgggcca
     2281 cgagatcgcg catgccttgc gcgagcacag ccgcgagcag gcttcgcagc aggcggcaca
     2341 gggcatcggc ctgtcggtga tcggcgccct cgccggactg ggcgatgccg ggatgtcgct
     2401 gtcgcagatg gcgatggacg tgaccttcaa cctgccgcac tcgcgccagg acgagcagga
     2461 ggccgaccgc atcggcgtcg agctcgccgc gcgagcgggc tacgatcccc gcgccgcgat
     2521 agcgctctgg gagaagatgc agaagctcgg cggcggccag ccgccgcaga tcctgtcgac
     2581 ccatccgtcc aacgagagcc gcatcgccga cctgaagcag tacgcggaaa aggtgatgcc
     2641 gctgtaccag caggcgaccg caaagcgctg atttcctgtg agagaatcca tgtttctgca
     2701 gcgcaaacat gggacagact ctctacgaca agttgtggga cagccacgtc gtccatgccg
     2761 aggacgacgg cacggcgctg ctctacatcg accggcacct ggttcatgaa gtcaccagcc
     2821 cgcaggccta cgaaggcctg aagctcgccg ggcgcaagcc ctggcgcatc ggctcggtcg
     2881 tcgccaccgc cgaccacaac accccgacgc ggggctggga gcgcggcgtc gagggcatcg
     2941 acgaccgctt cgccaagctg cagatccaga ccctcgacgc caacatgaag gagtacggcg
     3001 ccaaggtcta tttccccttc ctcgacaagc gccagggcat cgtgcacgtg atcggcccgg
     3061 agcagggcgc gacgctgccg gggatgaccg tggtgtgcgg cgattcgcac accagcacgc
     3121 acggcgcctt cggctgcctc gcgcacggca tcggcacttc ggaagtcgag cacgtgctcg
     3181 ccacccagtg cctgaccacc cgcaagatga aaaacatgcg cgtcagcgtc gacgggccgc
     3241 tcggccgcgg cgtcactgcg aaagacgtgg tgctcgccat catcggccgc atagggaccg
     3301 ccggcggcac cggctgcgcc atcgagtttg ccggcagcac catccgcgcg ctgtccatgg
     3361 aaggccgcat gacggtctgc aacatggcga tcgaagcggg cgcgcgcgcc ggcatgatcg
     3421 cggtcgacga tgtgacgatc gactatgtca aggggcgacc gttctcgccc agcggcccga
     3481 cctgggatgc cgccgccacc tactggcgca cgctgaagag cgacccggac gcgaagttcg
     3541 accgcgaggt gaagctcgac gcgcgcgaga tcaagccgca ggtcacctgg ggcacctcgc
     3601 ccgagatggt ggtgacggtg gacgacgccg tgcccgaccc cgagaaggag aaggacagcg
     3661 gccggcgcga aggcatggag cgcgcgctgg tctacatggg cctcaagccg cgcacccgca
     3721 tcaccgacat caagctcgac aagatcttca tcggctcgtg caccaactcg cgcatcgagg
     3781 acctgcgcgt ggcggccgcg gtggtcaaag gccggcgcgt ggcctcgaac atccgccagg
     3841 cgatggtggt gccgggctcg ggcctggtga aaacgcaggc cgagaaggaa ggtctagaca
     3901 aggtattccg cgacgccggc ttcgagtggc gcgagcccgg ctgctcgatg tgcctgggca
     3961 tgaacgacga tcgcctcggc gccggcgagc gctgcgcgtc gacctcgaac cgcaacttcg
     4021 agggccggca gggcgagggc ggccgcacgc atctggtcag tcctgccatg gcggcggcgg
     4081 ccgcgattgc cggtcacttc gtcgacatcc ggacctggcg ctgatggaaa aattcaccat
     4141 cctgaatgga cttgtcgcgc cgctcgaccg catcaacgtc gacaccgacc agatcattcc
     4201 gaaggagcat ctgaagtcga tcaagcgcac cggcttcgcc gacgcgctgt tcgccgcctg
     4261 gcgccatgat ccgcagttcg tcctcaaccg gccgcaatac aagggcgcga gcatcctgct
     4321 cgcgcgcaag aatttcggct gcggctcttc gcgcgagcat gcgccctggg cgctgaagga
     4381 ctacggcttc cgctgcatca tcgcgccgtc cttcgccgac atcttcttca ccaactgctt
     4441 caagaacggc ctgctgccgg tggcgctgcg cgaggacgag gtcgatcgcc tgttctacga
     4501 ggccgccgcc ttccccggtt tcaagctggt ggtgaatctg gaacagcaaa gcgtcgccac
     4561 gaccgacagc gcgcaggtga tgcgcttcga cgtcgaccct ttccgcaagt acacgctgat
     4621 caacggcctg gacgagatcg ggctcacgct gcgccacacc gagaagatcc gcgccttcga
     4681 ggcgcggcgc cagacgcagt ttccctggta cttctgatat gaaagtcgcg gttcttcccg
     4741 gtgacggcat cgggcccgag atcatttctc aggccttgat ggttttgaag aaactgcagc
     4801 tgggaattca gtttcaggag gcgcccgtcg gcggcgccgc gtatgccgcg cacggcgatc
     4861 cgctgccgga aaaaactctt tccttggcaa aacaggccga cgcggtcttg ttcggcgcgg
     4921 tcggcgaccc gaaatacgac agcctgccgc gcgccaagcg tcccgagcag gcgatcctcg
     4981 gcctgcgcaa ggagctcggc ctgttcgcca atctgcggcc ggcgcaggtt tttgcggagc
     5041 tggccgccgc ttcctcgctg aagcccgagg tggtggcggg gctcgacatc ctgatcgtgc
     5101 gcgagctgac cggcgacatc tacttcggcc agccaaaagg catcagagac aagggcggcg
     5161 agagggaggg cttcgacacc atgctctacc gcgagagcga agtgcggcgc atcgcgcggg
     5221 tcggcttcga ggcggcgcgc aagcgggcaa agaagctgtg ctcggtggac aaggcgaacg
     5281 tgctggaaac ctcgcagctc tggagagaag tcgtcaccga ggagtcgaaa aattacggcg
     5341 acgtgcagct gagccacatg tacgtcgaca actgcgcgat gcagctggtg cgcaatccga
     5401 agcagttcga cgtcatcgtc accggcaacc tgttcggcga catcctgtc
//
LOCUS       DASYJA010000218         1752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1757568, whole genome shotgun sequence.
ACCESSION   DASYJA010000218 DASYJA010000000
VERSION     DASYJA010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1757568"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VGO02_04680"
     CDS             complement(<1..752)
                     /locus_tag="VGO02_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEV7477017.1"
                     /translation="MNPIKKTFTYGNHQVTLETGEIARQAHGAVLVTMDDTVVLAAVT
                     ASKTAKPGQDFFPLTVDYAEKTYAAGKIPGGFFKREGRPTEKETLTSRLIDRPIRPLF
                     PDGFYNEVQVVAQVLSLNPEVDSDIPAMIGVSAALTLSGVPFNGPIGACRVGYIDGQY
                     ILNPTATQLKESKLNLVVAGTEQGVMMVESEARELPEDIMLGGVVYGHEQMQSAIQAI
                     NELAEVAGKPAWSFEPPAKDEALIARVKGLAEA"
     gene            complement(833..1102)
                     /gene="rpsO"
                     /locus_tag="VGO02_04685"
     CDS             complement(833..1102)
                     /gene="rpsO"
                     /locus_tag="VGO02_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEV7477018.1"
                     /translation="MALQVAQKAQVVKQYQRSGADTGSPEVQIALLTERINGLTEHFK
                     AHVKDFHSRRGLLKMVSQRRKLLDYLRSKDADKYRGLLERLNLRK"
     gene            complement(1174..>1752)
                     /locus_tag="VGO02_04690"
     CDS             complement(1174..>1752)
                     /locus_tag="VGO02_04690"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEV7477019.1"
                     /translation="LGRFRGEIEQIPPMYSALKRDGVPLYKLARRGEEVERKARRVSI
                     SQLDLMAFRSPLMELRVRCSKGTYIRTLAQDIGAALGCGAHLAALRRTGSGRFRIDDA
                     AGLEALAAMSPESRLRRLLPLAELLVGLPRAELDAPSAARLRNGQALPVAGLAQGLCG
                     VFGPDGAVLGLGRSDGASLHPLRLTQAPEKHR"
BASE COUNT          272 a          580 c          591 g          309 t
ORIGIN      
        1 gcttccgcga gccccttgac gcgcgcgatc agcgcctcgt ccttggccgg cggctcgaat
       61 gaccaggctg gcttgccggc gacttcggcc agctcgttga tcgcctggat cgcggactgc
      121 atctgctcgt ggccgtacac cacgccaccg agcatgatgt cttccggcag ctcgcgcgcc
      181 tccgactcga ccatcatcac gccctgctcc gtgccggcga cgaccaggtt gagcttcgac
      241 tccttcagct gggtcgccgt cgggttgagg atgtactggc cgtcgatgta gcccacgcgg
      301 cacgcgccga tgggcccgtt gaacggcacg ccggagaggg tcagcgcggc ggacacgccg
      361 atcatcgccg ggatgtcgga atccacctcg ggattgagcg acagcacctg cgccaccacc
      421 tgcacttcgt tgtagaagcc gtccgggaac agcggccgga tgggccggtc gatgaggcgc
      481 gaggtcagcg tctccttctc ggtggggcgg ccctcgcgct tgaagaagcc gccggggatc
      541 ttgcccgcgg cgtaggtctt ctcggcgtag tcgacggtca gcgggaagaa atcctgcccc
      601 ggcttggcgg ttttcgacgc ggtgaccgcg gcgaggacga ccgtgtcgtc catggtgacg
      661 agcacggcgc cgtgcgcctg gcgggcgatc tcgccggttt cgagcgtgac ctggtggttg
      721 ccgtaggtga aagttttctt gatcgggttc aaggggaaat cctcaatgag cgatgcgcaa
      781 acaaaaaacg ctcaccggga ggatgagcgc ttcatgctgc aacgtcgggg gcttacttgc
      841 gtaggttgag ccgctcgagc aggccgcggt acttgtccgc gtccttgctt cggaggtaat
      901 cgagcagctt gcgccgctgg ctcaccatct tgaggagccc gcgccgcgaa tggaagtctt
      961 tgacgtgcgc cttgaagtgc tcggtgagac cgttgatgcg ctcggtgagg agagcaatct
     1021 gcacttcggg cgacccggtg tcggcgcctg agcgctgata ctgtttcacc acttgcgctt
     1081 tctgcgcaac ctgcaatgcc attcgctatc tctctatgtc gtctttatct aaggggcggg
     1141 attctaaccc gaaggcgcgt cgcgccacaa ggtttagcga tgtttttcag gcgcttgggt
     1201 gagcctcaga ggatgcaggc tggcgccgtc gctgcggccc agaccgagca ccgcgccgtc
     1261 cgggccgaaa acaccgcaca gcccctgcgc caggcccgca accggcagcg cctggccgtt
     1321 gcgcagccgc gccgccgacg gtgcatcgag ctccgcgcgc ggcaggccga ccagcagctc
     1381 cgcgagcggc agcaggcgcc gcagccgcga ctcgggcgac atcgccgcca gcgcctcgag
     1441 gccggccgcg tcgtcgatac ggaaacgccc cgagccggtg cgcctgagcg ccgccaggtg
     1501 ggcgccgcag cccagcgcag cgccgatgtc ctgcgccagc gtgcggatat aggtgccttt
     1561 gctgcagcgg acccgtagtt ccatgagggg gctgcggaaa gccatgagat cgagctgcga
     1621 gatgctcacc cgccgcgcct tgcgctcgac ttcctcgccg cggcgcgcca gcttgtagag
     1681 cggcacgccg tcgcgcttca acgcggaata catcggcggg atctgctcga tttcgccccg
     1741 gaagcgcccc ag
//
LOCUS       DASYJA010000219         2320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_760137, whole genome shotgun sequence.
ACCESSION   DASYJA010000219 DASYJA010000000
VERSION     DASYJA010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760137"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VGO02_04695"
     CDS             <1..621
                     /locus_tag="VGO02_04695"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsD family peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEV7477020.1"
                     /translation="HVIDRELLVQKALEAGLERDPLVAHSLESARRQILAQAWLERRA
                     GGAAARPEEVRAFYGENPALFGERRIYRLRELAVSAPSELVDVLRGETHIAKDLDEVA
                     AWLRLRNAGVRPGSSVQPAEQLPLSYLPRLAQMKEGEIAVFDAPSGALVVQLMQAQEA
                     PLSEREAAPMIETFLSGRKRLEQAAAEVKRLREVARIEYVGEFKAR"
     gene            639..1385
                     /gene="epsE"
                     /locus_tag="VGO02_04700"
     CDS             639..1385
                     /gene="epsE"
                     /locus_tag="VGO02_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein EpsE"
                     /protein_id="HEV7477021.1"
                     /translation="MKVLVFAAAWLLAIPAWAQSGERLGVGDAIRVTVFQQPDLTTEV
                     RVSEKGTVAMPLIGEQKVAGKSTGEAAKQIADALKNGKYLKDPQVSIAMVTLRSRQVS
                     VLGQVARPGRYALDDTSSGLTEVLAAAGGVMPTGADSVTVVRDGQPQKVDVISKDFKL
                     KNGDTVYIERAPVFYIYGEVTRAGAYRVEPNMTVMQAIAAGGGITPRGSDRRLKMRRN
                     GADGKVVETDAKLQDMVKTDDVIYVKEALF"
     gene            1403..>2320
                     /locus_tag="VGO02_04705"
     CDS             1403..>2320
                     /locus_tag="VGO02_04705"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEV7477022.1"
                     /translation="MDFHQFMLALRARRKAFVMVFAAAIITAMAVALIVPKKYVSTAT
                     ILLDARDEQSMTAGRMSPRERAGYVSTQIDLIMSGRVAAKVARDLKLAQKPGMREAFE
                     SDTGGVGNIDDWIGANLLEKLKVDTSASNVVTVMFSSSDARASADIANGFAKAYLDTA
                     LALRTEPTREAAEWFNDQMKGMRTQVAQAQSKLAAYQKAKGIAFADERTDVDGARLSE
                     LSTQYLAARNATYEAISRQKQAAEALKGASADSIPEVLASGHISSLKVDLGRAEARLA
                     EMGEVLGPNHPQYQRTSAEVAGLRDKLASE"
BASE COUNT          415 a          787 c          820 g          298 t
ORIGIN      
        1 catgtcatcg accgcgagct gctggtgcag aaggcgctcg aggccggcct cgagcgcgac
       61 ccgctggtcg cgcactcgct ggagagcgcg cggcgccaga tcctggcgca ggcgtggctt
      121 gagcgccgcg ccggcggcgc ggccgcccgg ccggaggaag tacgcgcctt ctacggcgag
      181 aacccggctc tgttcggcga gcggcgcatc taccgcctgc gcgagctggc ggtgtcggcg
      241 ccgagcgagc tggtcgacgt gctgcgcggc gaaacgcaca tcgccaagga cctcgacgag
      301 gtcgccgcct ggctgcggct gcgcaacgcc ggcgtccgcc cgggcagctc ggtgcagccc
      361 gccgagcagc tgccgctcag ctacctgccg cgcctcgcgc agatgaagga aggcgagatc
      421 gcggtgttcg acgcgccctc gggcgccctg gtggtgcagc tgatgcaggc gcaggaagcg
      481 ccgctctccg agcgcgaggc ggcgcccatg atcgagacgt tcctctccgg gcgcaagcgc
      541 ctggagcagg cggcggctga ggtgaaacgg ctgcgcgagg tcgcgcggat cgaatacgta
      601 ggggaattca aagcgagata aagatgagag gccctctgat gaaagtcctt gtgtttgcag
      661 cagcgtggct cctggccatc ccggcctggg ctcaaagcgg cgagcggctg ggcgtcggcg
      721 acgccatccg cgtcaccgtg ttccagcagc ccgacctcac gacggaggtg cgcgtctcgg
      781 agaagggcac ggtggcgatg ccgctgatcg gcgagcagaa ggtcgccggc aagtccaccg
      841 gcgaggcagc caagcagatc gccgatgcgc tgaagaacgg caagtacctg aaggatccgc
      901 aggtgagcat cgccatggtc accctgcgca gccgccaggt ctcggtgctg ggccaggtgg
      961 cgcgcccggg gcgctacgcg ctcgacgaca cgagctcggg gctcaccgag gtgctggccg
     1021 ccgccggcgg cgtgatgccc accggcgccg acagcgtcac ggtggtgcgc gacggccagc
     1081 cgcagaaggt cgacgtcatc tccaaggact tcaagctgaa gaacggcgac acggtctaca
     1141 tcgagcgcgc gccggtgttc tacatctacg gcgaggtgac gcgcgccggc gcctaccgcg
     1201 tcgagcccaa catgaccgtg atgcaggcga tcgcggccgg cggcggcatc acgccgcgcg
     1261 gcagcgaccg gcggctcaag atgcgtcgca acggagccga cggcaaggtg gtcgagaccg
     1321 acgcaaaact gcaggacatg gtcaagaccg acgacgtgat ctacgtcaaa gaagcgctct
     1381 tctagaaggg agacagacac acatggactt ccatcaattc atgctggcac tgcgcgcacg
     1441 ccgcaaggcg ttcgtgatgg tcttcgccgc ggccatcatc accgccatgg cggtggcctt
     1501 gatcgtgccg aagaaatatg tctccaccgc cacgattctc ctcgacgcgc gcgacgagca
     1561 gtcgatgacc gccgggcgca tgtcgccgcg cgagcgcgcc ggctacgtct cgacgcagat
     1621 cgacctgatc atgagcggcc gggtagccgc caaggtggcg cgcgacctga agctcgcgca
     1681 gaagcccggc atgcgcgagg ccttcgagag cgacaccggc ggcgtcggca acatcgacga
     1741 ctggatcggc gccaacctgc tggagaagct caaggtggac acctcggcga gcaacgtggt
     1801 cacggtgatg ttcagctcct ccgacgcgcg cgcctcggcc gacatcgcca acggcttcgc
     1861 caaggcctac ctcgacaccg cgcttgcgct gcgcaccgag ccgacgcgcg aggcggccga
     1921 gtggttcaac gaccagatga agggcatgcg cacgcaggtg gcgcaggcgc agagcaagct
     1981 cgccgcctac cagaaggcga agggcatcgc cttcgccgac gagcgcaccg acgtcgacgg
     2041 cgcgcgcctg tccgagctct ccacccagta cctggcggcg aggaacgcca cctacgaggc
     2101 gatcagccgg cagaagcagg ccgccgaggc gctgaagggc gcctccgccg actcgatccc
     2161 cgaggtgctg gcgagcggcc acatcagctc gctgaaagtc gacctcggcc gggcggaagc
     2221 gcgcctcgcc gagatgggcg aagtgctcgg accgaaccat ccgcagtacc agcgcacctc
     2281 ggccgaggtg gccgggctgc gcgacaagct cgcctccgag
//
LOCUS       DASYJA010000220         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3120810, whole genome shotgun sequence.
ACCESSION   DASYJA010000220 DASYJA010000000
VERSION     DASYJA010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3120810"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VGO02_04710"
     CDS             complement(<1..429)
                     /locus_tag="VGO02_04710"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HEV7477023.1"
                     /translation="MIGVARLNEGLRAVALFEAFKGTLALLAAGGLFYLIPHDLRQVA
                     VELVGRLHLNAGKSYPNVFVRILEDTSNAQLWLVGCLVVIYALVRFAEAWGLWRSRRW
                     AEWLAAVSGAIYVPFELYELSRGFSWIKLAALAANLAVVAF"
     gene            complement(430..1038)
                     /gene="metW"
                     /locus_tag="VGO02_04715"
     CDS             complement(430..1038)
                     /gene="metW"
                     /locus_tag="VGO02_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEV7477024.1"
                     /translation="MTDQEQALLAARPDFAAIAQWVAPGARVLDLGCGDGSLLRYLWQ
                     ARNAPGYGVEIDDQRVLACVANDVNVLQVDLESGLSLFADGSFDCVVLSQTLQAMRHQ
                     EAILREMLRVGREVIVSFPNFGHWSARLQVLLGRMPVSETLPYEWYETPNLHHCTITD
                     FEDLCRKLGFRIRERLVLSQGKPVTVMPNLLGSLAVYRCTAT"
     gene            complement(1035..>2039)
                     /locus_tag="VGO02_04720"
     CDS             complement(1035..>2039)
                     /locus_tag="VGO02_04720"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006713310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEV7477025.1"
                     /translation="LNAARTNAVLVCHALNASHHVAGFYGDDPGNVGWWDNLVGPGKP
                     LDTRRFFVVGTNYLGSCFGSTGPASVNPATGKPWGADFPVVTVEDWVETQARLADRLG
                     IERFAAVIGGSLGAMQAMQWAMSYPDRVRHSIVVASAPKLSPQNIAFNEVARQAIMTD
                     PDFHGGHYYDKGVVPVRGLRIARMIGHITYLSGDAMTEKFGRLLRRGALGFDFDTDFE
                     VESYLRYQGDKFSTYFDANTYLRITKALDYFDPAADFGGDLSAALSRAKAAFLIVSFN
                     SDWRFTPARSREMVKALLDSRKIVSYLEVDAPGGHDAFLLEDPRYHSALRAYFATIDL
                     "
BASE COUNT          356 a          669 c          720 g          294 t
ORIGIN      
        1 gaatgcaacc accgcgaggt tagccgccag ggcggcgagc ttgatccatg aaaagccgcg
       61 ggagagctcg tagagttcga acgggacgta gatcgcaccg cttaccgcgg caagccattc
      121 ggcccagcgc cgcgaccgcc acagccccca cgcctcggcg aaccgcacca gcgcgtagat
      181 caccaccagg cagccgacca accagagctg cgcgttgctg gtgtcctcga gaatccgcac
      241 gaagacgttg gggtagctct tgccggcatt gaggtgcagc cggcccacca gctccaccgc
      301 aacctgccgc aggtcgtgcg ggatcaggta gaaaaggccg ccggcggcga gcagggcaag
      361 cgtccccttg aacgcttcga acagcgctac ggcgcgcaag ccctcgttca gccgcgcaac
      421 gcctatcact tacgtcgcgg tgcagcggta gacggcgagg ctgccgagga gattgggcat
      481 caccgtcacc ggcttgccct ggctcagcac cagacgctcg cggatgcgga agcccagctt
      541 gcggcacagg tcctcgaagt cggtgatggt gcagtggtgg aggttcggcg tctcgtacca
      601 ctcgtagggc agggtttccg acaccggcat gcggccgagc agcacctgca gccgcgcgct
      661 ccagtggccg aagttgggga agctgacgat gacttcgcgg ccgacgcgca gcatctcgcg
      721 caggatcgcc tcctggtggc gcatcgcctg cagggtctgc gacaacacca cgcagtcgaa
      781 ggagccgtcg gcgaagagcg acaggccgct ctccaggtcg acctgcagca cgttgacgtc
      841 gttcgcgacg caggcgagca ccctttggtc gtcgatttcg acaccgtagc ccggcgcgtt
      901 gcgcgcctgc cagaggtagc gcagcagcga gccgtcgccg caccccaggt cgagcacgcg
      961 cgcgccgggc gccacccact gcgcgatggc ggcgaaatcg ggacgggctg cgagcagcgc
     1021 ctgctcttga tcggtcacag gtcgatggtc gcgaagtagg cgcgcagcgc ggagtgatag
     1081 cgcggatcct cgagcaggaa ggcgtcgtgc ccgcccggcg cgtcgacctc gaggtaggag
     1141 acgatcttgc ggctgtcgag cagggccttc accatctcgc gcgagcgcgc cggcgtgaac
     1201 cgccagtcgg agttgaacga gacgatcagg aacgccgcct tggcgcgcga gagcgccgcc
     1261 gacaggtcgc cgccgaaatc cgccgccggg tcgaagtagt cgagcgcctt ggtgatgcgc
     1321 aggtaggtgt tggcgtcgaa gtaggtcgag aacttgtcgc cctggtagcg caggtaggac
     1381 tcgacctcga agtcggtgtc gaagtcgaag ccgagtgcgc cgcggcgcag gaggcggccg
     1441 aacttctccg tcatcgcgtc gcccgacagg taggtgatgt ggccgatcat gcgcgcgatg
     1501 cgcaggccgc gcaccggcac cacgcccttg tcgtagtagt gcccgccgtg gaaatcgggg
     1561 tcggtcatga tcgcctggcg cgccacctcg ttgaaggcga tgttctgcgg cgagagcttg
     1621 ggcgccgagg cgacgacgat ggaatggcgc acgcggtccg ggtaggacat cgcccactgc
     1681 atggcctgca tcgcgccgag cgagccgccg atcaccgcgg cgaagcgctc gatgcccagg
     1741 cggtcggcga ggcgcgcctg cgtctcgacc cagtcctcga ccgtgaccac cggaaagtcc
     1801 gcgccccacg gcttgccggt ggcggggttc accgacgccg ggccggtcga gccgaagcag
     1861 ctgccgaggt agttggtgcc gacgacgaag aagcgccgcg tgtcgagcgg cttgcccggg
     1921 ccgacgaggt tgtcccacca gccgacgttg cccggatcgt cgccatagaa gcccgcgaca
     1981 tggtgggagg cattgagcgc gtggcatacc agcacggcgt tggttcgagc cgcattgag
//
LOCUS       DASYJA010000221         2612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1123512, whole genome shotgun sequence.
ACCESSION   DASYJA010000221 DASYJA010000000
VERSION     DASYJA010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123512"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..404
                     /locus_tag="VGO02_04725"
     CDS             6..404
                     /locus_tag="VGO02_04725"
                     /inference="COORDINATES: protein motif:HMM:NF014674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477026.1"
                     /translation="MTGPLKAKLNREKLDFSQSKVSPNDLRELYGFVASGEISNSTAT
                     GVVLPAMWDGQGSPKEIIEKRGLKQISDSGEIEKLVDAAIAAGAKQVEDYRAGKEKAL
                     QSLVGQVMKASKGKANPAQVNEILRRKLAR"
     gene            complement(401..1090)
                     /locus_tag="VGO02_04730"
     CDS             complement(401..1090)
                     /locus_tag="VGO02_04730"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEV7477027.1"
                     /translation="MAKTLYQLLGLAPNAKPEAIKEAYVKHAARLRDSGATGEADVLK
                     QAYEVLSDPRLRARYDQQGYAVGGEVADADPEERHWLRSWKGALLAVAVLGVVFSAWS
                     YNKREQTRIRLEHERIEAQRFAAEQQRTEELRQREIERRAAEERDRTAADTAAAQRGN
                     QTSRSETLYRDTLQHQRTMQDDRLQLQRDRDAARREDLERRRLELENQRQLARDRRQA
                     QELERTSPRRF"
     gene            complement(1096..1938)
                     /locus_tag="VGO02_04735"
     CDS             complement(1096..1938)
                     /locus_tag="VGO02_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477028.1"
                     /translation="MSLIRLTICTAALALLPLAAAAQMQTTFRCQGTDGKKYYGNTIP
                     QQCYGRLVEQLNNQGMVIRRIEPEAKSNKDPEAEAAEKAAKEKLEAASRETMRRNRAL
                     LATYTSEKDIEEQRARALSDNHKAVREQEERVADLKKRRSGFDKELEFYQDKSGKTKV
                     PPKLQEEIRQADFDLKVQQDSLDVKKKEVVNINAKYDDDKHRFLVLSGKNPAERGAAL
                     GMDKGATVTSGKGPSAADEYHTKTEAQRRAMRERAEVERLEREREAARRRSAYQQQQQ
                     QQPR"
     gene            1997..>2612
                     /locus_tag="VGO02_04740"
     CDS             1997..>2612
                     /locus_tag="VGO02_04740"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEV7477029.1"
                     /translation="MHSADRAFIAETTAKMLLEVKAVLFFTDKPFVFTSGWASPVYID
                     CRKLIFYPRIRGQLVDFATDTLEREVGFEQFDVVAGGETAGIPYAAWIADRLGLPMQY
                     VRKKAKGFGRNAQIEGDIREGARTLLVEDLTTDGRSKINFCQALRTAGAVVDHVFVNF
                     YYDIFPESKKVMSELGVKLHYLATWWDVLAAVKKGGYLEAGKIAE"
BASE COUNT          383 a          855 c          868 g          506 t
ORIGIN      
        1 actggattac tggcccgctc aaggcgaaac taaatcgaga gaagctcgac ttctcccaaa
       61 gcaaggtctc cccgaacgac cttagagagc tatacggatt tgtcgccagt ggtgagatct
      121 ccaactcaac tgccactgga gttgtcctcc ccgccatgtg ggacggccag ggatcgccta
      181 aagagatcat cgaaaagcgc ggcctgaagc agatcagcga ctcgggcgag atagagaagc
      241 tggtcgacgc ggcgatcgcg gcgggggcga agcaggtgga ggactaccgc gccggcaagg
      301 agaaggcgct gcagtccctg gtcgggcagg tgatgaaggc gagcaagggg aaagccaatc
      361 cggcgcaggt gaacgagatc ctgcgccgca agctggcgcg ctagaagcgc cgcgggctgg
      421 tgcgctccag ctcctgggcc tggcgcctgt cgcgcgcgag ctggcgctgg ttctcgagct
      481 ccagccgccg gcgctcgagg tcctcgcgcc gcgcggcgtc gcgatcgcgc tgcaactgaa
      541 gccggtcgtc ctgcatggtt cgctggtgct ggagagtatc gcgatagagc gtctcgctgc
      601 gcgacgtctg gttgccgcgc tgcgctgccg cggtatcggc ggcggtgcga tcgcgttcct
      661 cggcggccct gcgctcgatt tcgcgctggc ggagctcctc ggtccgctgc tgctccgcgg
      721 cgaagcgctg tgcctcgatc cgctcgtgct cgaggcggat gcgcgtctgc tcgcgcttgt
      781 tgtaggacca ggcggagaag acgacgccga ggaccgctac ggcgagcagc gcgcctttcc
      841 atgaacgcag ccaatggcgt tcctccgggt cggcgtctgc aacctcgccg ccgacggcat
      901 aaccctgctg gtcgtagcgc gcccgcaggc gtgggtcgga aagcacctcg taggcctgct
      961 tcagcacgtc ggcctcgcct gtcgcgcccg aatcgcgcag ccgcgcggcg tgcttgacgt
     1021 aggcctcctt gatcgcctcc ggcttggcgt tgggcgcaag gccgagcagc tggtagagcg
     1081 tctttgccat ggcggctagc gcggctgctg ttgctgttgc tgctggtacg ccgagcgtct
     1141 gcgcgccgct tcccgctccc gctcgaggcg ctccacttcg gcgcgctccc gcatggcgcg
     1201 gcgctgcgcc tcggtcttgg tgtggtactc gtccgcggcc gatggcccct tcccgctggt
     1261 gacggtcgcc cccttgtcca tgcccagggc cgcgccgcgc tcggccgggt tcttgccgct
     1321 cagcacgagg aagcggtgct tgtcgtcgtc gtacttcgcg ttgatgttga cgacttcctt
     1381 cttcttcacg tcgagggagt cctgctgcac cttgaggtcg aagtcggcct ggcggatctc
     1441 ctcctgcagc ttgggcggta ctttcgtctt gcccgacttg tcctggtaga actcgagctc
     1501 cttgtcgaag cccgaacggc gctttttcag gtcggcgacg cgctcttcct gctcgcgcac
     1561 cgccttgtgg ttgtcggaga gcgcgcgggc gcgctgctcc tcgatgtcct tctcgctggt
     1621 gtaggtggcg agcagcgcgc ggttgcggcg catcgtctcg cggctcgcgg cctcgagctt
     1681 ctccttggcc gccttttcgg ccgcctctgc ctccggatcc ttgttgctct tggcctcggg
     1741 ctcgatgcgc cggatgacca tgccctggtt gttgagctgc tcgaccaggc ggccgtagca
     1801 ctgctgcggg atggtgttgc cgtagtactt cttgccgtcg gttccctggc agcggaacgt
     1861 ggtctgcatt tgcgcggccg cggcgagggg cagcagcgcg agcgcggcgg tacaaatcgt
     1921 cagccttatc agagacatcc ccatccccca atgcggatcg tgactgaatt ctaacctgtt
     1981 aaaatccgcg ccgcctatgc actccgccga ccgcgcattt atcgccgaaa ccaccgcgaa
     2041 aatgctgctg gaggtgaaag ctgtgctgtt tttcacggac aagccgttcg tcttcacctc
     2101 gggctgggcg agcccggtgt acatcgactg ccgcaagctc atcttttatc cacgcatccg
     2161 cggccagctg gtcgacttcg ctaccgacac gctcgagcgc gaggtgggct tcgagcagtt
     2221 cgatgtcgtc gccggcggcg agacggccgg catcccctac gcggcctgga tcgccgatcg
     2281 cctcggcctg ccgatgcagt acgtcaggaa gaaggcgaaa ggcttcggcc gcaacgcaca
     2341 gatcgagggc gacatccgcg agggcgcgcg caccctgctg gtcgaggacc tgaccaccga
     2401 cggccgctcc aagatcaact tctgccaggc cctgcgcacg gccggtgcgg tggtcgatca
     2461 cgtcttcgtc aacttctact acgacatctt ccccgagtcg aaaaaggtga tgagcgagct
     2521 cggcgtcaag ctgcactacc tcgctacctg gtgggacgtg ctggcggcgg tgaagaaggg
     2581 cggctacctc gaggccggca agatcgccga gg
//
LOCUS       DASYJA010000222         2662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_124230, whole genome shotgun sequence.
ACCESSION   DASYJA010000222 DASYJA010000000
VERSION     DASYJA010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124230"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VGO02_04745"
     CDS             <1..668
                     /locus_tag="VGO02_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477030.1"
                     /translation="EYVDAFTFTRFEPSGTVQGNDSIKFATSILDYVFRELAVSYLER
                     FDLAHVDPTDGGFDALGKGVEEGKPGSGPASTRYVSKGLTRSRTDKLSVLAGGATTSS
                     APPSSLAGEGRGGGNVTAFGGRETRAEIAGSAALKAEPEHRLSPTEQLEQQHLPFVRA
                     DARGDVADARAGARATAAERRAEAKARGYEGEACGECGNFTLVRNGTCLKCDTCGGTT
                     GCS"
     gene            complement(983..1639)
                     /locus_tag="VGO02_04750"
     CDS             complement(983..1639)
                     /locus_tag="VGO02_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477031.1"
                     /translation="MRVGSLQRQRKSSILPVIGGCLAVYAVCAVGFHALVEPSLGKNQ
                     PPPAPVLQYSAAALAARAEAVTPSPVAVSRPASTAVAAAPAARAAAPKVTAKVTPAAP
                     PAVAPTPAVAAAPAPAAAPAPAVALAPAVALAPSATAALAPEAASTAAGAATPEATAD
                     TVEKTAAVEPKKPARKRVAAPKPQPSFFDFFSGGFNNNGRANNARSSTARSTGPRSFF
                     "
     gene            1890..>2662
                     /locus_tag="VGO02_04755"
     CDS             1890..>2662
                     /locus_tag="VGO02_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEV7477032.1"
                     /translation="MLAKSDARRIWLRAQRLDRTAPFGDGPQATPAAVAHLGYVQIDT
                     INVIERSHHHILYSRIPGYRREHLHQAQTIDKTVFEYWTHALSYIPTADMRFYVGAMK
                     RHWQRRSVWFTSVTPADLRKVLRRIRTLGALTIRDIDDDVLVEKNHAWASRKPSKRAL
                     QLAFYRGLLTVSERKGMLKTYELMTRHFGWDRLPRAAPERDILNYLLDRALRSQGVVS
                     LDSACYLDPRRKPAMARLIESRMRRKELVPVELAGAGKL"
BASE COUNT          471 a          911 c          873 g          407 t
ORIGIN      
        1 aagaatatgt cgatgccttc accttcacgc gcttcgaacc gtccgggacg gtccaaggca
       61 acgactcgat caagttcgcc acctcgattc tcgattacgt cttccgcgag ctcgcggtga
      121 gctacctcga gcgcttcgac ctcgcccatg tcgatccgac cgatggcggc ttcgacgcgc
      181 tcggcaaggg cgtcgaagaa ggcaagcccg gcagcggccc agcctcgacc agatacgtct
      241 ccaaggggct cacgcgctcg cgcacggaca agctttcggt tctggcagga ggtgcgacga
      301 cctcctccgc cccgccctcc tccctcgcgg gagagggtcg gggtgggggg aatgtcaccg
      361 ccttcggcgg ccgcgagacc cgcgccgaaa tcgccgggtc agccgcgctc aaggccgagc
      421 ccgagcacag gctctccccc accgagcagc tcgagcaaca gcacctgccg ttcgttcgcg
      481 cggatgcgcg cggtgatgtt gctgacgctc gcgcaggcgc gcgagccacg gctgccgagc
      541 ggcgggccga agccaaggcc aggggctacg aaggcgaagc ctgcggcgaa tgcggcaact
      601 tcacgctggt ccgcaacggc acgtgcctca agtgcgacac ctgtggcggg acgaccgggt
      661 gtagttaggc tcgcgcagcc ataacaactg caagggcgat cgctcacccg atcgcccttt
      721 ttatttcagc ttgtagggcg aaatagcgaa gcgtattgcg ccactgacgg tcgcggtcgc
      781 gatgcagcga tcaacgcctc ggctgcgcta caattggagt cgatgacccg acgcagcggg
      841 aagcatcgga ccacggccaa caaaactccc attgcggagc cgggggtctc gggtggaggg
      901 gaaggacttc gcgcaaaccg aaatagcgcg gtgcctcgcc tcgatacggg agcggttcag
      961 gcgactgcct caggcagccg gactagaaga aagagcgggg tccggtggag cgagcggtgc
     1021 tggagcgagc gttgttggcc cgaccgttgt tgttgaagcc gcctgagaag aagtcaaaga
     1081 agcttggctg cggcttgggc gctgccacgc gcttgcgggc tggcttcttc ggttcgacag
     1141 cagcggtctt ttccacggtg tccgctgtcg cttccggcgt cgcggcccct gccgctgtcg
     1201 acgccgcttc gggtgccaat gccgctgttg ctgagggcgc cagagccact gccggagcca
     1261 gagccactgc cggggccgga gccgctgccg gggccggagc cgctgccact gccggggtcg
     1321 gggccactgc cgggggcgcc gccggcgtca ctttcgccgt cactttcggc gccgctgcgc
     1381 gcgccgctgg cgctgccgcg acggcggtcg acgccggccg gctcactgcc acgggagacg
     1441 gcgtcaccgc ctcagcgcgg gccgccaacg cggcggccga gtattgcaga acgggtgcgg
     1501 gcggcggctg gttcttgccc agcgagggct ctaccagcgc atgaaagccg acggcgcaga
     1561 cggcatagac cgccaggcag ccgccgatca ccggcaggat ggatgatttc cgttgacgct
     1621 gaagactgcc gacgcgcatt gcaaaatccc cccaacaaca agcagcccga agggcttccc
     1681 gacaattagt cgcagggacg gccaaactgg ttcaatccgg ccgcaaaatt cgcacctccc
     1741 gccccagccc ccgaccctcc taagcgaacc ggttcgcggc ggaaaacgcc gttaacacgg
     1801 ggaatcgggg cagtttccgg agcgacgaat ccgaatcgcg ccgttgtaca ctcccgtcgc
     1861 ccatgccccg cccgaccaag actcccttcg tgctcgccaa atccgatgcc agacgcatct
     1921 ggctgcgagc ccagcggctc gaccgtaccg cgccgttcgg cgacgggccg caggcgaccc
     1981 ccgcggcggt ggcgcatctg ggctacgtcc agatcgacac catcaacgtg atcgagcgca
     2041 gtcatcacca catcctctat tcgcgcattc cgggataccg gcgcgagcac ctccaccagg
     2101 cccagaccat cgacaagacg gtgttcgagt actggacgca cgccttgtcg tacattccga
     2161 ccgccgacat gcggttctac gtcggcgcca tgaagcggca ctggcagcgc cgcagcgtct
     2221 ggttcacctc ggtgacgccg gcggatctgc gcaaggtgct ccgccgcatc agaacgctgg
     2281 gcgcgctgac gatccgggac atcgacgacg acgtgctagt cgaaaagaat cacgcctggg
     2341 cgagccgcaa gccgtcgaag cgcgcgctgc aactggcgtt ctaccgcggc ctcctgaccg
     2401 tcagcgagcg gaagggcatg ctcaagacct acgagctgat gacccggcac ttcggctggg
     2461 atcggttgcc gagggccgct ccggaacgcg acatcctgaa ctatctgctc gaccgcgccc
     2521 tgcgctcgca aggcgtcgtc agcctggatt cggcctgcta tctcgacccc cggcgcaagc
     2581 cggcgatggc tcgattgatc gagagccgaa tgcgtcgcaa ggaactggtg ccggtcgagc
     2641 tcgcgggcgc cggcaagctg ca
//
LOCUS       DASYJA010000223         5325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1850063, whole genome shotgun sequence.
ACCESSION   DASYJA010000223 DASYJA010000000
VERSION     DASYJA010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850063"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VGO02_04760"
     CDS             <1..796
                     /locus_tag="VGO02_04760"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7477033.1"
                     /translation="RVYGYDPLSKARAALNRAGGVALESPLAVFSKVKILITSLPSAK
                     ALHAVCKEVEGKGAVVIETSTLPIKEKIKARDTLKKKGITLLDCPLSGTGAQAKVGDL
                     VVFGSGDKAAFSKVVSTLKGFSRGHHYLGEFGNGSKMKFVANLMVAIHNVSAGEAFAL
                     GIKAGLAPQAIFDVQANSAGTSRMFQVRGPMMVKGRYDDVTMKNEVWQKDMKIIGDYA
                     RSLGVATPTFKASAGVYKAAMAQGFARQDTGAVCAVLERMSGVRRR"
     gene            complement(800..1693)
                     /locus_tag="VGO02_04765"
     CDS             complement(800..1693)
                     /locus_tag="VGO02_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEV7477034.1"
                     /translation="MTSRLRLYLLVAGSLVPLIGFAVIAARQLVQHERAAVEREAIGR
                     ARSAMSAVDAHLRNSIVALETLGASKSLEAGNIAAFHQEAQRVLRTQPAWVNIGLASA
                     ERKVQLFNAVYALGKPEPIVTQEEAFTAALRGAKATFSNVAAGTAVQSPTVRVRVAVS
                     YADEVRYVLSAPQNLKLFAELMQSQQLPDDWGIALVDRETRVITRIPAVPAGVEESDS
                     LRDAIRRAPAGWFEGQSRDGRPTYTAYVTSQLSGWVLGVAIPAGTVEAGEQRILTMLA
                     AGMLLSLVIGMALAWQVAERR"
     gene            1782..2324
                     /locus_tag="VGO02_04770"
     CDS             1782..2324
                     /locus_tag="VGO02_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV7477035.1"
                     /translation="MIKRVARFRKLKGSDGGLPDSPLPECRRKLYAVIGFSPPEDDSN
                     AAVTSPVGDDASSKPAIAIAEGFNLGYCKAKPGKGPLMHNHDTNETFIAMTGRWRCEW
                     NEGNEMEHVDLAPYDVISFPTGVARRFMNVTYDEPGREHLLMFIIGGNQPQAEFTPLA
                     MRRCEEFQNKKKTKTKRRKA"
     gene            2324..4675
                     /locus_tag="VGO02_04775"
     CDS             2324..4675
                     /locus_tag="VGO02_04775"
                     /inference="COORDINATES: protein motif:HMM:NF018620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid cis/trans isomerase"
                     /protein_id="HEV7477036.1"
                     /translation="MRAWLPVLLLALAGCATLYSAQLNQRYGAPDPARYDRPAAATGA
                     DYAPVKAILDNRCAVCHGCNDAPCQLNLASYAGLTRGANPEQVYATRFLTAEPTRLFF
                     DAQSSAAWRAKGFYPVLNERAATPEADREAGVLYRVLRLKRAHPLPAGAVLPKERFDF
                     ALDRAQQCPAIEGMDQYERKYPDWGMPFGLPGLSQAEHDTLARWIESGARSSPATLLA
                     PAYAQRVARWEAFLNGDSLKARLASRYIFEHWFVAHLYFDDLPRGEHGDEFGDAFFEL
                     VRSKTPPGQPIQVIATRRPYDDPGVERAYYRLRRVGETLLAKTHMPYALNDARMARLR
                     EWFLDAPYEVSALPSYVPAVASNPFIAFQQIPVEARYRLMLEESQFTLMGFIKGPVCR
                     GQVAVDVINDHFWVMFEQPGAGRAELTAEFLAREFANMRLPAEEEGAGPLLKWRRFAA
                     HEAAYLKAKSAALERAFPGGPTLDLLWDGDGRNPNAALTVVRHFDSASVVQGLLGERP
                     QTVMLIGYQLLERIHYLLVAGFDVYGNVGHQLTTRLYMDFLRTEGEMNFFAFLPLASR
                     QAVHDRWYRGADRASIAQLWDTQAYYQNETGVRYATSDPLAELYGLMKKRAAAVTSPR
                     YALASSGLTGAPLRELDRLATLRGRALSFLPEAAILTVRDASGERHFSLIANRAHSNV
                     AELFDEAERRLPDEDSLLVANGFIPAYPNAFFAVDASKLSEFVDAVARLASEPDYSSL
                     MKTWGIRRADERFWAHSDALHAAWRRSAPREAGLFDYNRLENR"
     gene            complement(4651..>5325)
                     /locus_tag="VGO02_04780"
     CDS             complement(4651..>5325)
                     /locus_tag="VGO02_04780"
                     /inference="COORDINATES: protein motif:HMM:NF015552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477037.1"
                     /translation="TLVAGGLGPYAPGFASALWWLGCVLLTVFQVCAIGRWLAGQVQL
                     DKLNAGWLILIVGGIVVPGPGVSLGHAEAARFFFGVSACGAVVLLPLLLARAALAPPL
                     PEALRPTWFILLVPPSLIYANGLALYPDALLLENLFFAGLALVLALALYARALWRWPF
                     GPAWWAFTFPLDALAYAATRYAQEHPSGPWKAVAGAALLAATLLVATVLARTLLGARH
                     LFSRRL"
BASE COUNT          928 a         1844 c         1766 g          787 t
ORIGIN      
        1 cagggtttac ggctacgacc cgctctccaa ggctcgcgcc gcgctcaacc gtgcaggcgg
       61 ggttgcactt gaatctccct tggcggtttt ttccaaggtg aagattctca ttacgtcact
      121 ccccagcgcc aaggcgctgc acgccgtctg caaggaagtc gaagggaaag gggccgtggt
      181 catcgagacc agcacgctgc cgatcaagga aaagatcaag gcgcgcgaca cactgaagaa
      241 gaagggcatc accctcctcg actgcccgct ctccggcacc ggcgcgcagg cgaaggtcgg
      301 cgacctggtc gtgttcggca gcggggacaa ggccgccttt tcaaaggtcg tctccacatt
      361 gaagggcttt tcccgcggac accactatct gggcgagttc ggcaacggca gcaagatgaa
      421 gttcgtcgcc aacctgatgg tggcgatcca caacgtctcg gccggcgagg cgttcgcgct
      481 aggcatcaag gccggcctcg cgccgcaggc gatcttcgac gtccaggcga acagcgccgg
      541 cacctcgcgc atgttccagg tgcgcggccc gatgatggtg aagggccgct atgacgacgt
      601 gacgatgaag aacgaggtct ggcagaagga catgaagatc atcggcgact acgcgcgcag
      661 cctcggcgtg gcgacgccga ccttcaaggc ctccgccggg gtctacaagg ccgccatggc
      721 gcagggcttc gccaggcagg ataccggcgc ggtgtgcgcg gtgctggaaa gaatgagcgg
      781 cgtgcggcgc cgctgagggt cagcgccgct ccgccacctg ccacgcgagc gccatgccga
      841 tgacgaggga aagcagcatg ccggccgcca gcatggtcag gatgcgctgc tcgccggcct
      901 cgaccgtgcc tgcaggaatc gcgacgccga ggacccagcc gctcagctgg gaggtgacgt
      961 aggcggtgta ggtggggcgg ccgtcgcggc tctgaccctc gaaccagccc gcgggcgcgc
     1021 gccggatcgc atcgcgcaag ctgtccgact cctcgactcc ggcgggcacg gccgggatgc
     1081 gcgtaatgac gcgcgtctcg cggtcgacca gggcgattcc ccagtcgtcg ggcaattgct
     1141 gcgactgcat cagctcggcg aagagcttca ggttctgcgg cgccgaaagc acgtagcgca
     1201 cctcgtcggc gtacgacacc gccacgcgca cccgcaccgt ggggctctgc accgcggtgc
     1261 cggccgcgac gttgctgaag gtcgcctttg cgccgcgcag cgcggcggtg aaggcttcct
     1321 cttgggtaac gatcggctca ggcttgccca aggcatagac ggcgttgaac agttgcacct
     1381 ttctttccgc cgaggcgagc ccgatgttga cccaggcggg ctgcgtgcgc agcacacgct
     1441 gcgcctcctg gtggaacgcc gcgatattgc ccgcctcgag gctcttcgag gcgccgaggg
     1501 tctcgagcgc gacgatcgag ttgcgcaggt gagcgtccac cgcgctcatc gccgagcgcg
     1561 cccgcccgat cgcctcgcgc tcgacggccg cccgctcgtg ctgcaccagc tgccgggccg
     1621 cgatgacggc gaagccgatc agcggcacca gcgagccggc cacgaggagg tacaggcgca
     1681 agcgcgatgt catgggcctt ctataataac tgtcgcgcca cgacgaggag cgaatcgaaa
     1741 tgcaatcctg gaaaaacatc cgcacttgga cccgcgcgga catgataaag cgcgtagcgc
     1801 gcttcaggaa gctgaaaggc tccgacggcg ggctgcccga cagcccgctt cctgaatgcc
     1861 ggcgcaagct ttacgccgtc atcggctttt ctccgcccga agacgacagc aatgcggcag
     1921 tcacctcccc ggtcggcgat gacgcctcgt cgaagccggc gatcgccata gccgagggct
     1981 tcaacctcgg ctactgcaag gcgaagcccg gcaaagggcc gctgatgcac aaccacgaca
     2041 ccaacgagac cttcatcgcc atgaccgggc gctggcgctg cgaatggaac gagggtaatg
     2101 agatggagca tgtcgatctc gcgccttatg acgtgatctc cttccccacc ggcgtggcgc
     2161 ggcgcttcat gaacgtgacc tacgacgagc ccggccgcga gcacctgctg atgttcatca
     2221 tcggcggcaa ccagccgcag gcggaattca ccccgctggc gatgcggcgt tgcgaggaat
     2281 tccagaacaa gaagaagacg aagaccaaac ggcgcaaagc ctgatgcgcg cctggctgcc
     2341 ggtcctgctg ctcgcgctgg ccggctgcgc cactctctat tccgcgcagc tgaaccagcg
     2401 ctatggcgcg cccgatccgg cgcgctacga tcgccccgcg gcggccaccg gcgcggacta
     2461 tgcgccggtc aaggccatcc tcgacaaccg ctgcgccgtc tgccacggct gtaacgacgc
     2521 accctgccag ctcaacctcg cctcctacgc agggctgacg cgcggcgcca accctgagca
     2581 ggtgtatgcc acgcggttcc tcaccgccga gccgacccgg ctattcttcg acgcgcagag
     2641 cagcgccgcg tggcgcgcga agggtttcta tccggtactg aacgagcgcg ccgcgacccc
     2701 cgaagctgac cgcgaggccg gcgtgctcta ccgggtgctg cgcctcaagc gcgcccatcc
     2761 tctgccggcg ggtgcggtgt tgcccaagga acgtttcgat ttcgccctcg atcgcgcgca
     2821 gcagtgcccg gcgatcgaag gcatggacca gtacgagcgc aagtatcccg actggggcat
     2881 gccgttcggt ctgccgggcc tgtcgcaggc cgagcacgac acgctggccc gttggatcga
     2941 gtccggcgca cgctcgtccc ccgcgacgct gctggcaccg gcctatgcgc agcgcgtcgc
     3001 gcgctgggag gccttcctca acggcgacag cctcaaggcg cgcctggcga gccgatatat
     3061 cttcgagcac tggttcgtcg cacaccttta cttcgacgac ctgccgagag gggagcacgg
     3121 ggacgaattc ggagacgcat tcttcgagct tgtgcgctcg aagaccccac cggggcagcc
     3181 aatccaggtc atcgccacgc gccggcccta cgacgatcca ggagtcgagc gcgcgtacta
     3241 ccggctgcgc cgcgtcggcg aaacgctgct cgccaagacc cacatgccct acgcgctgaa
     3301 cgacgcgcgc atggcgcgcc tcagggaatg gttcctcgac gccccctacg aggtgagcgc
     3361 cctgccctcg tatgttcccg cggtggcttc caatcctttc atcgccttcc agcagatccc
     3421 cgtcgaggcg cgctatcgcc tgatgctcga ggagtcgcag ttcaccctga tgggcttcat
     3481 caagggcccg gtgtgccgcg gccaggtggc ggtcgacgtg atcaacgacc acttctgggt
     3541 gatgttcgag caacctggcg ccggtcgcgc cgagctcacc gcggaattcc tcgcgcgcga
     3601 attcgccaat atgcgcctgc cggccgaaga ggaaggcgcc ggcccgctgt tgaaatggcg
     3661 gcgcttcgcg gcgcacgagg ccgcctatct gaaggcgaaa agcgcggcgc tggagcgggc
     3721 cttccccggc gggccgacgc tggacctgct gtgggacggc gacggccgca accctaacgc
     3781 cgcgctcacc gtggtgcgcc atttcgacag cgcctcggtg gtgcagggtc tgctcggcga
     3841 gcggccgcag accgtcatgc tcatcggcta tcagctgctc gagcgcatcc actacctgct
     3901 ggtggcggga ttcgacgtct acggcaatgt cggtcaccag ctgaccacgc gcctgtacat
     3961 ggacttcctg cgcaccgaag gcgagatgaa tttcttcgcc ttcctgccgc tcgccagccg
     4021 gcaggcggtg cacgaccgct ggtatcgcgg cgccgatcgc gcgtcgatcg cgcagctatg
     4081 ggacacgcag gcctattacc agaatgagac cggtgtgcgc tacgccacct ccgatccgct
     4141 cgccgagctc tacggtctga tgaaaaagcg cgcggcggca gtaaccagcc cgcgctacgc
     4201 gctcgcctcg agcggcctca cgggcgcacc gctgcgcgag ctggaccggc tcgccacgct
     4261 gcgcggccgc gcgctgtcgt ttcttccgga agccgcgatc ctgaccgtgc gggatgcgtc
     4321 cggcgagcgc cacttcagcc tgatcgccaa ccgcgcgcac agcaacgtcg cggagctgtt
     4381 cgacgaggcc gagcgccgcc tgcccgacga ggacagcctg ctcgtggcga acggcttcat
     4441 cccggcctac ccgaatgcgt tcttcgccgt ggatgcttcg aaattgtccg agttcgtcga
     4501 tgccgtcgcg cggctggctt ccgagccgga ttactcatcc ttgatgaaaa cctggggaat
     4561 ccgccgcgcc gacgagcgct tctgggcaca cagcgacgcg ctgcacgccg cctggcgccg
     4621 ctcggcgccg cgggaagccg gcctgttcga ctacaaccgc ctcgagaaca ggtgacgcgc
     4681 gcctagcagg gttctcgcca gcactgtcgc cacgagcaga gtggcagcga gcagcgccgc
     4741 gccggcaacc gccttccacg gccctgaagg gtgctcctgc gcgtagcgcg tggcggcata
     4801 ggccagcgcg tcgagcggga aggtgaaggc ccaccaggcc ggaccgaacg gccagcgcca
     4861 caaggcgcgc gcgtagagcg ccagcgcgag gacgagcgcc agcccggcga agaacaggtt
     4921 ttccaggagc agcgcgtccg gatagagcgc gaggccgttg gcgtagatca gcgacggcgg
     4981 cacgagcagg atgaaccacg tcggccgcag cgcctcgggc agcggggggg caagcgcggc
     5041 gcgcgcgagc agcagcggca gcaggactac ggcgccgcag gcggaaacgc cgaagaagaa
     5101 gcgcgccgcc tcggcgtggc cgagcgagac gcccggtccc ggcaccacga tgccgccgac
     5161 gatcaggatc agccagccgg cgttcagctt gtcgagctgc acctgcccgg caagccagcg
     5221 gccgatggcg cagacctgga atacggtgag gagcacgcac cccagccacc acagcgcgct
     5281 tgcgaaaccg ggggcgtacg gccccaggcc acccgccacg agggt
//
LOCUS       DASYJA010000224         2822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3212523, whole genome shotgun sequence.
ACCESSION   DASYJA010000224 DASYJA010000000
VERSION     DASYJA010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3212523"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VGO02_04785"
     CDS             <1..432
                     /locus_tag="VGO02_04785"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7477038.1"
                     /translation="VKGLLDNTPVSAGGYIAHVAVPCRDLGEAASWYAKVLGAQPVRI
                     LKDRVTFSFGGVLQLVCHLERRAVAANPRAYPRHHGLTFLRMEDFSRLKQHIEKLGVK
                     FLVRPAVRFANSAHEHQTFMVVDPSGNVVEFKCYVRPECSY"
     gene            complement(426..1070)
                     /locus_tag="VGO02_04790"
     CDS             complement(426..1070)
                     /locus_tag="VGO02_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEV7477039.1"
                     /translation="MKEYLEPAQFIDSTHAAVVEFSRKNSSGNSERERAVSLYYAVRD
                     EIRYNPFLDFSDREVFRASTVLAADQGFCVGKASLLAACARAAGIAARVGFADVKNHL
                     TSPRLAETMGSDLFVYHGYADLYLDGKWVKATPAFNLDLCTRFRVKPLEFDGRQDSIF
                     HPFDQDNRRHMEYLRERGTYADVPVEAIQQAFREAYPKFYRLGRDAAKETFAPQ"
     gene            complement(1067..1921)
                     /locus_tag="VGO02_04795"
     CDS             complement(1067..1921)
                     /locus_tag="VGO02_04795"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7477040.1"
                     /translation="MNAPDNALLARQYNNRELVPDHAQYFARWGEASARARSTMTSHL
                     DLRYGDMPGETIDLFPARKGDGSCMMFIHGGYWRSLDKKDFSFLAPALVDGGISLAVV
                     NYDLCPTVTMEQIVQQMLRASRWLWLHAEEYGMDQDRLYASGHSAGGHLTAMLMCAVW
                     PAFDAQLPKDLWKGGLAVSGLYDLRPLLQVDFLQQDLRLDEAAAVKLSPAFMSPATRA
                     PLMTSVGGAESGEFLRQNRLLGERWRAAFAGDVPMPGKNHFSVADGLAERSSPLFQAA
                     LRLMKLDK"
     gene            complement(1918..>2822)
                     /gene="gtdA"
                     /locus_tag="VGO02_04800"
     CDS             complement(1918..>2822)
                     /gene="gtdA"
                     /locus_tag="VGO02_04800"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HEV7477041.1"
                     /translation="WKYREARPYLMEAGTLISAKEAVRRVLILENPGMRGESCITQSL
                     YAGLQLILPGEIAPSHRHSQSALRLIVEGSGAYTAVDGERTTMRPGDFIITPSWTWHD
                     HGHPGAEPVVWMDGLDIRIVQTFAAQFHEVYPEEEQPVTRSEGASVARYGANLAPVGA
                     RPPFGKTSPIFNYPYERSREALAKMAQDQAADACHGWKMEYINPTSGGHAMPTIAAFL
                     QLLPKGFRSQAYRCTDGTIYSVVEGQGTAVIGNEAFDFEPRDTFVVPSWAPLQLESKG
                     ETVLFSFSDRPGQEAMGLWREKRG"
BASE COUNT          450 a          910 c          988 g          474 t
ORIGIN      
        1 gtcaaaggcc tgctggacaa caccccggtc agcgctggag gctacatcgc gcacgtcgcc
       61 gtcccatgcc gcgacctcgg ggaagccgcc agctggtacg cgaaagtcct cggcgcgcag
      121 ccggtacgga tcctcaagga ccgcgtcacg ttcagcttcg gcggcgtcct gcagctggta
      181 tgccacctgg agcggcgcgc ggtcgcggcc aatccgcgcg cctacccgcg gcaccacggc
      241 ctgacgttcc tgcgcatgga ggatttttcc aggctgaagc agcatatcga gaagctgggc
      301 gtcaaattcc tggtgcgccc cgcggttcga ttcgccaaca gcgcccacga gcaccagacc
      361 ttcatggtgg tcgatccttc cggcaacgtg gtggagttca agtgctacgt ccggccggag
      421 tgcagttact gaggcgcgaa ggtttccttg gccgcgtccc ggccgagccg atagaacttg
      481 ggataggcct cgcggaacgc ctgctggatg gcttcgaccg gcacgtcggc gtaggtgccg
      541 cgctcgcgca ggtactccat gtggcggcgg ttgtcctggt cgaacggatg gaagatcgag
      601 tcctggcggc cgtcgaactc gagcggcttg acgcgaaagc gcgtgcacag atccaggttg
      661 aacgccggcg tcgcctttac ccatttgccg tcgaggtaga gatcggcgta gccgtggtag
      721 acgaagaggt cgctgcccat ggtctcggcc aggcgcggcg aagtgaggtg gtttttcacg
      781 tcggcgaagc cgacgcgggc cgcgatgccc gcggcgcgcg cgcaggcggc cagcagcgag
      841 gccttgccga cgcagaatcc ctgatcggcc gcgagcacgg tcgaggcgcg gaacacttcg
      901 cgatccgaga aatcgagaaa cgggttgtag cggatttcgt cgcgcaccgc gtaatacagc
      961 gagactgcgc gctcgcgctc tgaatttccg gaagagtttt ttctggagaa ttcaactacg
     1021 gcagcatgcg tgctgtcgat gaactgcgcg ggctcgagat attccttcat ttgtcgagct
     1081 tcatcaggcg cagggcagcc tggaagaggg ggctcgagcg ctcggcgagg ccgtcggcga
     1141 cggagaaatg gttcttgccc ggcatcggca cgtcgccggc gaacgcggcg cgccagcgct
     1201 cgccgaggag gcgattctgg cgcaggaatt cgcccgactc ggcgccgccg acgctggtca
     1261 tcagcggggc gcgggtggcg ggcgacatga acgccggcga gagcttcacc gcggctgcct
     1321 cgtccaggcg caggtcctgc tgcaggaagt cgacctgcag gagcggtcgc aggtcgtaga
     1381 gcccgctcac cgcgaggccg cccttccaca ggtccttcgg cagctgggcg tcgaacgccg
     1441 gccataccgc gcacatcagc atcgccgtga gatgcccgcc ggccgagtgg ccggaagcat
     1501 agagccggtc ctggtccatg ccgtattcct cggcgtgcag ccacagccag cggctcgcgc
     1561 gcagcatctg ctgcacgatc tgctccatcg tcaccgtggg gcacaggtcg tagttgacca
     1621 ccgcgagcga gatgccgccg tcgaccagcg ccggcgcgag gaacgagaag tccttcttgt
     1681 cgagcgagcg ccagtagccg ccgtggatga acatcatgca gctgccgtcg cccttgcgcg
     1741 cggggaacag gtcgatggtc tcgcccggca tgtcgccgta gcgcaggtcg aggtggctgg
     1801 tcatggtgct gcgcgcgcgc gccgaagcct cgccccagcg ggcgaagtac tgcgcgtgat
     1861 cgggaaccag ctcgcggttg ttgtattgcc gggcgagcag ggcgttgtcg ggagcgttca
     1921 tccccgcttc tcgcgccaca ggcccatcgc ttcctggccg gggcggtcgg agaaggagaa
     1981 cagcacggtc tcacccttgg actcgagctg cagcggcgcc cacgagggca cgacgaaggt
     2041 gtcgcgcggc tcgaaatcga atgcttcatt tccaataacg gcggttccct gtccttcgac
     2101 gacggaatag atcgtgccgt cggtgcagcg gtaggcctgc gacctgaagc ccttcggcag
     2161 gagctgcagg aacgcggcga tggtcggcat ggcgtggccg ccgctcgtcg ggttgatgta
     2221 ctccatcttc cagccgtggc aggcgtcggc agcctggtcc tgcgccatct tggcgagcgc
     2281 ttcgcggctg cgctcgtagg gatagttgaa gatcggcgag gtcttgccga agggcggcct
     2341 ggccccgacc ggcgccaggt tcgcgccata gcgcgccacc gaggcgccct cgctgcgcgt
     2401 caccggctgc tcttcctccg ggtacacctc gtggaactgc gccgcgaagg tctgcacgat
     2461 gcgaatgtcg aggccgtcca tccagaccac cggctcggct ccgggatggc cgtggtcgtg
     2521 ccaggtccac gacggcgtga tgatgaaatc gccggggcgc atggtggtgc gctcgccgtc
     2581 gaccgcggtg taggcgcccg agccttcgac gatcaggcgc agcgccgact gcgagtggcg
     2641 gtggctgggg gcgatctcgc ccggcaggat cagctgcagg ccggcgtaca ggctctgcgt
     2701 gatgcacgac tcgccgcgca tgcccgggtt ctcgaggatc aggacgcggc gcaccgcctc
     2761 cttcgcgctg atcagcgtgc cggcttccat caggtagggg cgcgcctcgc ggtacttcca
     2821 ca
//
LOCUS       DASYJA010000225         3873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2484497, whole genome shotgun sequence.
ACCESSION   DASYJA010000225 DASYJA010000000
VERSION     DASYJA010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2484497"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VGO02_04805"
     CDS             complement(<1..410)
                     /locus_tag="VGO02_04805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV7477042.1"
                     /translation="MKLAIVRQRYNPYGGAERIVARALPALERAGAQVTLIARSDKGW
                     GARRVLQVNPFYVGNLWRDWSFARAARRTWRREGFDLVQSHERIPGCDVYRAGDGVHR
                     RWLELRRAAAGPLERLGMALNPYHRYVCGTEKRM"
     gene            complement(407..1480)
                     /gene="rfaQ"
                     /locus_tag="VGO02_04810"
     CDS             complement(407..1480)
                     /gene="rfaQ"
                     /locus_tag="VGO02_04810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipopolysaccharide heptosyltransferase
                     III"
                     /protein_id="HEV7477043.1"
                     /translation="MKDAVPLGECRRALVLKLRHHGDVLLAAPVLSVLKAHAPQLELD
                     ALVYDDTAPMLEGHPALSQLHTVGRGWRQLDPLSRFFEEKKLLGGLRERRYDLIVHLS
                     EQPRGAWLARWLGARYSVAPKMADRGRFWQRSFTHFYPLVPRRHMVEVNLDALRRIGV
                     QPTLHERKVTFVPGAPAEQAVDRLLDGRPFIHLHPASRWRFKCWSIEKNAELIDRLAA
                     EGHQLVLTAAPDETGMIDEILGKARARPLNLAGKLSLKQLGALTARARLFVGVDSMPM
                     HLAAAMGVPVVALFGPSGESEWGPWSAACRVVTSSVHTCRPCGQDGCGGGKVSECLTR
                     LPVDAVHAAARELLAESPAAAVK"
     gene            complement(1533..3488)
                     /locus_tag="VGO02_04815"
     CDS             complement(1533..3488)
                     /locus_tag="VGO02_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV7477044.1"
                     /translation="MLRTLAGVLAGVFVSVSFAQDDAVIVTATRFADSKRNLPVGVTV
                     ITADDLRKSATSNLPEILAQYGLLHIRDNSGSPNQGIDLRGFGITGDQNTLILVDGVR
                     LSENEVVPAQLSSIPLESIERIEVVRGSGAVLYGGGASGGTINIITKRGEDARRGYVL
                     GRFGGWGTKEGRAGYATQGETFGFSLDASTEDTNGYRNNNNYRQNNLSGGLEARAATG
                     RAYLRFNAYEQELGLPGQLSEAQILADPRQAATPNNESEARGGTVILGGSWKPGRHEL
                     AADLSRREKHSTSFFGPTLFSITPSTTDTRVGVTALQPRAKLAFDALGRSHDVVVGID
                     WEEWDFKSQVTGDFPSLRTGEQTSQAVYVQANLWLAQRTRLVLGGRSQRTEDHLKEEV
                     AVSDDRRAAYNLEAYEAALRQGFGAGWSAYGKYGKSFRVATFDDNACFFPPCAVTLLK
                     PQTALGGELGIEYESKGWRARGAYYSQDLENEIYFSSLAGANINLQPTRRRGYELEAA
                     WRATPTLDLRASVAQMEAKFKSGVYGGVDVTGKDVPLVPQTIATAGFAWSFVPRTRFI
                     ANARYVGQQRYDNDQANTFHKQPDYTLVDLKLEHTSGPFTVALEVKNLFDEGYYSYGI
                     KNFAGTSFDAYPAPGRAGYVTLAYRLP"
     regulatory      complement(3524..3702)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          569 a         1359 c         1308 g          637 t
ORIGIN      
        1 aacatgcgct tctcggtccc gcagacgtag cggtggtagg gattgagggc catgcccagg
       61 cgctcgagcg gcccggccgc ggcccggcgc agctcgagcc agcggcgatg cacgccgtcg
      121 ccggcgcggt acacgtcgca gccggggatg cgctcgtgcg actgcaccag atcgaacccc
      181 tcccgccgcc aggtcctgcg cgcggcgcgg gcgaacgacc agtcgcgcca caggttgccg
      241 acgtagaagg ggttcacctg cagcacgcgg cgcgcgcccc agcctttgtc ggagcgggcg
      301 atcagcgtca cctgggcgcc cgcacgctcc agcgccggca gcgcgcgggc cacgatgcgc
      361 tcggcgccac cgtaggggtt gtagcgctgg cgcacgatgg ccagcttcat ttgaccgcag
      421 cggcgggcga ctcggccagc agctcgcgcg cggccgcatg caccgcgtcg accggcagcc
      481 gcgtcaggca ctcgctcacc ttgccgccgc cgcagccgtc ctggccgcac ggccggcagg
      541 tatgcacgct gctggtcact acgcggcacg ccgcgctcca gggcccccat tcgctttcgc
      601 cgctcggccc gaacagcgcg accaccggca cccccatcgc ggcggcgagg tgcatcggca
      661 tcgagtcgac gccgacgaag agccgcgccc gcgcggtaag cgcgccgagc tgcttgagcg
      721 agagcttgcc ggccaggttg aggggccgcg cgcgggcctt gccgaggatc tcgtcgatca
      781 tcccggtctc gtcgggcgcc gcggtcagca ccagctggtg gccttcggcg gccaaccggt
      841 cgatcagctc cgcgttcttc tcgatcgacc agcacttgaa gcgccagcgc gacgcgggat
      901 gcaggtggat gaaaggccgg ccgtcgagga gcctgtcgac ggcctgctcg gccggcgcgc
      961 ccgggacgaa ggtcaccttg cgctcgtgca gcgtgggctg cacgccgatg cgccgcaggg
     1021 cgtcgaggtt gacctcgacc atgtggcgcc gcggcacgag cggatagaaa tgcgtgaagc
     1081 tgcgctgcca gaagcggccg cgatcggcca tcttcggcgc cacgctgtag cgcgcgccga
     1141 gccagcgcgc gagccatgcg ccgcgcggct gctccgagag gtgcacgatg aggtcatagc
     1201 ggcgctcgcg cagcccgcca agaagtttct tctcttcgaa aaacctgctc agcggatcta
     1261 gctggcgcca gccgcgcccc accgtgtgca attgggagag cgccggatgg ccttcgagca
     1321 tcggcgcggt gtcgtcgtag accagcgcgt cgagctccag ctgcggcgcg tgcgccttca
     1381 gcacgctcag caccggcgcc gcgagcagca cgtcgccgtg gtgccgcagc ttcagcacca
     1441 gcgcgcgccg gcactccccc agcgggacgg cgtccttcac catcccgcga gtgtactagg
     1501 ggaggaccgg caggaccggc tcccagccca cttcagggca ggcggtaggc cagcgtcacg
     1561 taccccgcac gtcccggcgc gggatacgcg tcgaagctgg tgccggcgaa gttcttgatg
     1621 ccgtagctgt agtaaccctc gtcaaagagg ttcttcactt ccagcgcgac cgtgaacggc
     1681 ccgctggtgt gctcgagctt cagatcgacc agcgtgtaat cgggctgctt gtggaaggta
     1741 ttcgcctggt cgttgtcgta gcgctgctgt ccgacgtagc gcgcgttggc gatgaaccgc
     1801 gtgcgcggga caaacgacca cgcgaaaccg gccgtggcga tggtctgcgg cacgagcggc
     1861 acgtccttgc ccgtcacgtc gacgccgccg tagaccccgc tcttgaattt cgcttccatc
     1921 tgcgcaaccg aagcgcgcag atcgagcgtg ggcgtcgccc gccatgccgc ctccagctcg
     1981 tatccccgac ggcgcgtggg ctgcaggttg atattggcgc ccgccagcga gctgaagtag
     2041 atctcgttct ccaggtcctg cgagtagtag gcgccccgcg cgcgccagcc tttactctcg
     2101 tactcgatgc ccagttcgcc gcccagtgcg gtctgcggct tgagaagcgt caccgcgcag
     2161 ggcgggaaga agcaggcgtt gtcgtcgaat gtggcgacgc ggaagctttt cccgtatttg
     2221 ccgtaggccg accagccggc gccgaagccc tgccgcagcg cggcctcata cgcctccagg
     2281 ttgtacgcgg cgcggcgatc gtcgctgacc gcgacctctt ccttgaggtg gtcctcggtc
     2341 cgctggctgc gcccaccgag gaccagccgc gtgcgctgcg ccagccagag gttggcctgc
     2401 acgtagactg cctggctggt ctgctcgccg gtgcgcagcg aggggaagtc cccggtcacc
     2461 tggctcttga agtcccactc ctcccaatcg atgccgacca ccacatcgtg gctgcgtccc
     2521 agcgcgtcga acgccagctt ggcgcgcggc tgcagcgccg tcaccccgac gcgcgtgtcg
     2581 gtggtggaag gcgtgatgga gaaaagcgtc ggaccgaaga acgacgtgct gtgcttttcg
     2641 cggcgcgaaa gatccgccgc cagctcgtgg cgacccggct tccaggatcc gccgagtatc
     2701 accgtgccgc cccgcgcctc gctttcgttg tttggcgtgg ccgcctggcg gggatcggcc
     2761 agaatctgcg cttcgctgag ctgtcccgga agtccgagct cctgctcata ggcgttgaat
     2821 cgcaggtagg cgcgcccggt tgcggcgcgg gcctcgaggc cgcccgagag gttgttctgg
     2881 cggtagttgt tgttgttgcg gtagccgttg gtgtcttcgg tcgatgcgtc cagcgaaaac
     2941 ccgaatgtct cgccttgcgt ggcatagcca gcgcgccctt ccttggtgcc ccagccgccg
     3001 aagcggccga gcacgtagcc gcgccgcgcg tcctcgccgc gcttcgtgat gatgttgatg
     3061 gtgccgccgc tcgcgccccc gccgtagagc accgcgccgc tgccgcgcac gacctcgatg
     3121 cgctcgatcg actcgagcgg gatggagctc agctgcgccg gtaccacctc gttctcggaa
     3181 agcctcactc cgtcgaccag gatcagcgtg ttctggtcgc ccgtgatgcc gaagccgcgc
     3241 aggtcgatcc cctggttcgg cgagcccgag ttgtcgcgga tgtgcagcag gccgtactgc
     3301 gccaggatct ccggcaggtt cgaagtggcg ctcttcctca ggtcgtcggc ggtaatcacc
     3361 gtgaccccga ccggcaggtt gcgcttggaa tcggcgaagc gcgtcgccgt gacgatgacc
     3421 gcatcgtcct gggcaaagga aacagaaacg aaaacgccgg cgagcacgcc agctagagtg
     3481 cgcagcattg aagcctccct ggcgtcatcc ccgacgcctc agcgattgag aaaggcttcc
     3541 aggccggtct ccggactcac gagtcttgat gcccgccttc ccgcgcgttg ccgcgcagtg
     3601 gcttggttgg gcaccctcac tcgcttaccg ttgcgggggc agtacgggct tctcacctgt
     3661 ttcccgaaca cccgaaagcg agccgatatc ataaccgcat gcggtttccg gcgcttctcg
     3721 tctgcctcct gtacccgagc ctgcttttct gccaggtggc ggtgcgcgac gactacgaca
     3781 aagaggtcag gctggcgggc ccggccggcc ggatcgtcag cctcgcgccg catctcaccg
     3841 agttgctgta cgccgccggc gccggcccgc gcc
//
LOCUS       DASYJA010000226         2322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_760896, whole genome shotgun sequence.
ACCESSION   DASYJA010000226 DASYJA010000000
VERSION     DASYJA010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760896"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="VGO02_04820"
     CDS             <1..848
                     /locus_tag="VGO02_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HEV7477045.1"
                     /translation="GEVRFVVEMKIDEIVDWLWEDLKLPDLQPKRSETMDEPDYEREG
                     WDKRGARSRLDRRRTLKEAIKRKSVQDNAVAIVNDDLRFRQLVRRPTPSTKAAVIFAL
                     DVSGSMDEGQRRLAKQFFFFALQGLRRQYAKVETAFIAHAAEAWEFDESQFFQASSSG
                     GTVSSCAFELAGEILRGRYDAGRYNQYLFYASDGENAAEDRGRAAAALRAMSETLNYA
                     GYVETGGVATFRPRSTQLTELFRELHEQGLPVGLSGLGAQDDVWRAIREFFGSRQDEP
                     ARAVR"
     gene            823..2181
                     /locus_tag="VGO02_04825"
     CDS             823..2181
                     /locus_tag="VGO02_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEV7477046.1"
                     /translation="MNPLAQYAEGLERQARLRGLDYYPVDFEIAPQSLMTEIAVYGLP
                     VRMPHWSFGVRYIHQLVRQSMGNSKIFEVMFPGDPCRAFLMDGNSLAENTLVTAHVLG
                     HADFAKNNQLFARFHEMSGGNIVEQAAAHALRIQRAINEVGVERVEAVLDAALALESH
                     VDVSGELHRSRYPEFVSEKQKISKSEFQKRFGSLPGESRPQEVATGPLRTAIPPYPEY
                     DLLWFIAHYAPDLEPWERDIFLMVRAESLYFYPVFACQIMNEGWASYWHARLLREADF
                     LPQPTYLEALRAHSDVVRPFAASEQTALAVNPYHLGFTIWERIVEEHGLERAREVRRD
                     EDDFGFIRNWLDRGLAEEAGLLVYEEGADEEIRVASRDLTAIREAILAPKFNYGAPRV
                     AAVALAADGSLDLVHDHAADGRGLDAARARQVLGYVRRMWRRAVRLQTTDGSGDRIEL
                     AA"
BASE COUNT          388 a          816 c          776 g          342 t
ORIGIN      
        1 acggcgaggt gcgcttcgtc gtcgagatga agatcgacga gattgtcgac tggctgtggg
       61 aggacctgaa gctccccgac ctgcagccca agcgcagcga gacgatggac gagccggact
      121 acgagcgcga gggctgggac aagcgtggcg cgcgctcccg cctcgatcgc aggcggaccc
      181 tgaaagaggc gataaaaaga aaatccgtgc aagacaacgc cgtcgccatc gtcaacgacg
      241 acctgcgctt ccgccagctg gtaaggcggc cgacaccgtc gaccaaggcg gcggtgatct
      301 tcgccctcga cgtctcgggc agcatggacg aggggcagcg gcgcctcgcc aagcagttct
      361 tcttcttcgc gctgcagggc ctgcgccgcc agtacgccaa ggtcgagacc gcgttcatcg
      421 cccacgccgc cgaggcctgg gaattcgacg agagccagtt cttccaggcc tcctcgagcg
      481 gcggcacggt gtcctcctgc gccttcgagc tggccggcga gatcctgcgc ggccgctacg
      541 acgcgggccg ctacaaccag tacctgttct acgcctccga cggcgagaac gcggccgagg
      601 accgcggccg cgccgccgcg gcgctgcgcg ccatgagcga gacgctcaac tatgccggct
      661 acgtggagac cggcggcgtc gccactttcc ggccgcgctc gacgcagctc acggagctct
      721 tccgcgagct gcacgaacag ggcctgccgg tcggcttgag cggcctcggc gcgcaggacg
      781 acgtgtggcg cgcgatccgc gaattcttcg gcagcaggca ggatgaaccc gctcgcgcag
      841 tacgctgagg gcctcgagcg ccaggcgcgg ctgcgcggcc tggactacta cccggtcgac
      901 ttcgagatcg cgccgcagag cctgatgacc gagatcgcgg tctacggcct gccggtgcgc
      961 atgccgcact ggtccttcgg cgtgcgctac atccaccagc tggtgcgcca gagcatgggc
     1021 aactcgaaaa tcttcgaggt catgttcccg ggcgacccct gccgcgcctt cctcatggac
     1081 ggcaactcgc tggccgagaa cacgctggtc accgcgcacg tcctgggcca cgccgacttc
     1141 gccaagaaca accagctgtt cgcgcgcttc cacgagatgt cgggcggcaa catcgtcgag
     1201 caggcggcgg cgcacgcgct gcgcatccag cgcgcgatca acgaggtggg cgtggagcgc
     1261 gtcgaagccg tgctcgacgc ggcgctcgcc ctggaatcgc acgtcgacgt ctcgggcgag
     1321 ctgcaccggt cgcgctatcc cgagtttgtt tccgagaaac agaaaatcag caaatccgaa
     1381 tttcaaaagc gcttcgggtc gctgcccggc gaaagccggc cgcaggaggt cgcgaccggc
     1441 ccgctgcgca ctgcaattcc gccgtacccg gaatacgacc tgctctggtt catcgcgcac
     1501 tacgcgcccg acctggagcc gtgggagcgc gacatcttcc tcatggtgcg cgccgagtcg
     1561 ctgtacttct acccggtgtt cgcctgccag atcatgaacg agggctgggc ctcgtactgg
     1621 cacgcccggc tgctgcgcga ggccgacttc ctgccgcagc cgacctacct cgaggcgctc
     1681 agggcgcact cggacgtggt gcggcccttc gccgccagcg agcagaccgc gcttgccgtc
     1741 aacccctacc acctcggctt caccatctgg gagcgcatcg tggaggagca cggcctcgag
     1801 cgcgcgcgcg aggtgcgccg cgacgaggac gatttcggct tcatccgcaa ctggctcgac
     1861 cgcggcctcg ccgaggaggc cggcctgctg gtgtacgagg aaggcgccga cgaggagatc
     1921 cgcgtcgcga gccgcgacct caccgccatc cgcgaggcga tcctcgcgcc gaagttcaac
     1981 tacggcgcgc cgcgcgtcgc ggcggtcgcc ctggcggccg acggctcgct cgacctcgtg
     2041 cacgaccacg ccgccgacgg gcgcggcctg gacgcggcgc gcgcgcgcca ggtcctcggc
     2101 tacgtgcggc ggatgtggcg ccgcgccgtc cgcctgcaga ccacggacgg cagcggcgac
     2161 aggatagagc tagctgctta aagaataata attacaagca gtatgacgat gatgatcagc
     2221 agccattcct gcgtggtgac gtcgccgccg gcgggcacgg cgttgagccg gccggcgagg
     2281 ctgcgcacct cctcatcggt catggcatcg atccgcccct ga
//
LOCUS       DASYJA010000227         4696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_124319, whole genome shotgun sequence.
ACCESSION   DASYJA010000227 DASYJA010000000
VERSION     DASYJA010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124319"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VGO02_04830"
     CDS             <1..492
                     /locus_tag="VGO02_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477047.1"
                     /translation="LPLVHQLVRYLARYEQTPSWLTVGQVVDLASIAAQIRGDRIVVT
                     PSGQRTTQRANAPGLLELTEQGVYEVRAAASTTGRPMAIAVNIDPAEADLAPVDPAEL
                     VAAVTGRATADTADAAAPEPLSREDAEKRQALWWYLLFAGVLLLAAETVISNHLSRKE
                     KFL"
     gene            799..4323
                     /locus_tag="VGO02_04835"
     CDS             799..4323
                     /locus_tag="VGO02_04835"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HEV7477048.1"
                     /translation="MVAVLLLSASSLQAASFSVPAIIGFRLLALAAFAALLAYALVPL
                     KRQVTDTQVALYLEEHNPSLEAAILSAVESSETAKESQSQQLVERLVQQAIAQSRAID
                     YGMAIDRPRMKRHAITLATFAAAAALFIALGPAYIRSGLSALLIFYHSAEAASPYKIE
                     VTPGDASVPRGGDQSVHAKLKGFVSKDVTLMMRGDGATEFERLPLVAGADADSFEGMM
                     FRLDKDTTYFVESNGVTSTHHKLTVVDLPTVKQLDVEYHFPAYTNLPPRKEENGGDVA
                     ALRGTSVLLHITPTMKTSGGQILFKEGAAVPLSVQADGTLAGNFTLSQPGFYRIELIG
                     PKGEKVEASPQYTIDVIDDQPPTVSFGKPGRDTSASPVEEVFTEVKAADDFGIKQLQM
                     VYSVNGGPQKTIALFGGGKTLTEVSAGHTIYLEELGLQPGDFVSYFAKATDNDSVPGP
                     HVASSDIYFVQVRPFKKDYKQAQSQAQQGGGGGGGGDVGELSRQQREIVAATFNTVRD
                     KDKTKADKYRENVVFLNLAQAKLRMQVEELLAKLKDRLGALDQDSEFRSIAEQLPKAA
                     AEMAQAESDLKAMKPNDALAPEQRALKILQVVEQQYETQIAQQQGGGGGGGQQSQMAE
                     DLADLFELELDKLANQYEMKQQAQQQASDQQIDQLAEKLKELARRQQQEAERQARLSQ
                     AGSQQQQQAGGGGGGSQRQLAQEAEEAARRLEQLSREQKRPDLAEAARQLQDAANAMR
                     QAAANGTQDNGALAKSALDKLKDAQQKLAQEMNGRSQRDIQDALRKSQELAAEQKDVA
                     SEVNQLEGQQGTDRRVKAQQLALRKFTMDNKLGELQSDLEKLANDARKDEKDASRKLD
                     EAAGSIRDKRVREKIRYSRSVLQGATPQSAPGMEEDISANLDALEKKIAEAQGAIGNK
                     SKQDGLAKAADQARDLVRGMESLDQRIRDRANQQARNGQQQQGKEGEQGKEGQQAKEG
                     QQGKEGQQGKEGQQGKEGQQGKEGQQGKEGQQGKGGQQGKEGQQGQAQGQEGQQGGAR
                     GGDANRAPQVGAYGGDARPDGANYGGRFTADDVRQFTRQFQELNRDAQQLRQQLQAAG
                     INPADLDAVMRGLTQGQQASIYDDPNALAKLQAAALERLKQFEFNLRKKLETGGESLA
                     LSGSDEVPAGFRQAIEEYYRALAKRATK"
     gene            4434..4510
                     /locus_tag="VGO02_04840"
     tRNA            4434..4510
                     /locus_tag="VGO02_04840"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4468..4470,aa:Pro,seq:tgg)
BASE COUNT          948 a         1474 c         1580 g          694 t
ORIGIN      
        1 cttccgctcg tccatcagct ggtgcgctat ctcgcccggt acgaacagac gccgtcgtgg
       61 ctcaccgtcg gccaggttgt cgacctggca tccatcgccg cgcagattcg gggcgaccga
      121 atcgtcgtca ctccctcggg ccagcgcacc acgcagcggg ccaatgctcc gggactcctc
      181 gaactgaccg agcagggggt gtatgaagtc cgtgctgccg cctcgacaac cggtcggccg
      241 atggcgattg ccgtgaacat cgatccggcc gaagccgacc tcgcccccgt ggatccggcc
      301 gagctcgtcg ccgcggtgac tggccgagcg accgccgaca cggcggacgc cgccgccccc
      361 gagccgctgt cgcgtgaaga tgccgagaaa cggcaggcgc tgtggtggta tctgctcttc
      421 gccggggtgc tgttgctggc tgccgaaact gtcatttcga accatttgtc cagaaaagag
      481 aaatttctgt agcgcggccg ttttggcccg ttttgggcct gtcaggtggt aatctttttc
      541 tctgagcttt ctcggttctt tttgctgatt ggacgtgacg tctatctgtc tgaatatggc
      601 gtctaattac cagatgttgc gtctaagact gctcgtttaa atgcacggcg agtctaatgg
      661 aacgtagggt ccggcttcag ccggattgag gggaccgaca tggaccatcg cgcagagctc
      721 gtcgacgtca ttcgaggcat ccgcaaccgc tggcgcctcc gcctggcggt ccggggcgcg
      781 gtcatcgtct tcgtggggat ggtggccgtg ctgctgctgt cggcatccag cctgcaggcg
      841 gccagtttca gcgtcccggc gatcatcggg ttccgcctgc tcgcactggc agcgttcgcg
      901 gcgctcctcg cctacgcgct cgtgccgctg aagcggcagg taaccgacac gcaggtcgcg
      961 ctctacctcg aagagcacaa tccctcgctc gaagcggcga ttctgagcgc ggttgaatcc
     1021 tcggagacgg cgaaagagag ccagtctcag caactggtcg agcggctggt gcagcaggcc
     1081 atcgcgcaga gccgcgccat cgattacggc atggcgatcg atcgcccccg catgaagcgg
     1141 cacgcgatca cgctggcgac atttgccgcc gcggcggcgc tcttcatcgc gctcggcccc
     1201 gcctacatca ggagcggcct ttcggctctg ctgatcttct accacagcgc cgaagcggcg
     1261 agcccctaca agatcgaggt cacgcccggc gacgccagcg tgccgcgcgg cggcgatcag
     1321 tcggtgcacg cgaagctgaa agggttcgtg tcgaaagacg tcacgctgat gatgcgcggc
     1381 gacggcgcga ccgagttcga gcgcctgccg cttgtcgccg gtgcggatgc cgactcgttc
     1441 gaaggcatga tgttccgcct cgataaggac accacgtact tcgtcgaatc gaacggcgtc
     1501 acatcgacgc accacaagct gacggttgtc gatctaccga ccgtcaagca gctcgacgtg
     1561 gagtaccact tccctgctta cacgaacctg ccgccgcgca aagaggaaaa cggcggcgac
     1621 gtcgccgcgc tgcgcggcac cagcgtgctg ctccacatca cgccgacgat gaagacgtcc
     1681 ggcggacaga ttctgttcaa ggaaggcgcc gcggtgccgt tgtccgtgca ggcggacggc
     1741 acgctggccg gcaacttcac gctatcgcag cccggcttct accgcatcga gctcatcggc
     1801 cccaagggcg agaaggtcga agcctcgccg cagtacacga ttgatgtgat cgacgatcag
     1861 ccgccgacgg tgtcgttcgg caagccgggt cgcgacacca gcgcgagccc tgtcgaggaa
     1921 gtcttcaccg aggtgaaggc ggccgacgat ttcgggatca agcagctgca gatggtgtac
     1981 tcggtgaacg gcggcccgca gaagaccatc gcgctcttcg gcggcggcaa aacgctcacc
     2041 gaggtgagcg ccgggcacac gatttacctg gaagagctcg gcctccagcc cggcgacttc
     2101 gtgtcctact tcgccaaggc caccgacaac gactcggtcc ccggtccaca cgtcgcgtcg
     2161 agcgacatct acttcgtgca ggtccgcccg ttcaagaagg attacaagca ggcccagtcg
     2221 caggcgcagc agggcggcgg cggcggtggg ggcggcgacg tcggcgagct ctcgcgccag
     2281 cagcgcgaga tcgtcgcggc gaccttcaac accgttcgtg acaaggacaa gaccaaagcg
     2341 gacaagtacc gcgagaacgt cgtcttcctg aatctcgcgc aggcgaagct tcgtatgcag
     2401 gtcgaggagc tcctcgcgaa gctgaaagac cggctcggcg ccctcgatca ggattccgag
     2461 ttccgatcga tcgcggagca gctgccgaag gcagccgcgg agatggcgca ggccgaatcg
     2521 gatctgaagg cgatgaagcc caacgacgcg ctcgcgcccg agcagcgcgc gttgaagatc
     2581 ctgcaggtcg tcgagcagca gtacgaaacg caaatcgccc agcagcaggg cggcggaggc
     2641 gggggcgggc aacagagcca gatggccgag gacctggccg atctcttcga gctcgagctc
     2701 gacaagctgg cgaatcaata cgagatgaag cagcaggccc agcagcaggc cagcgaccag
     2761 cagatcgatc agctcgcgga aaaactgaag gagctggcgc ggcgccagca gcaggaagcc
     2821 gagcgccagg cccgtctctc gcaggccggc tcgcagcaac agcagcaggc gggcggcggc
     2881 gggggcggca gccagcggca gctcgcacag gaagccgagg aagcggcgcg ccggctcgaa
     2941 cagctgtcgc gcgagcagaa gcggcccgac ctcgccgagg cggcgcgtca gctgcaggat
     3001 gcggccaacg cgatgcggca ggcagcggcc aacggcacgc aggacaacgg cgcgctggcc
     3061 aagtccgcgc tcgacaaact gaaagacgcg cagcagaagc tggcccagga aatgaacggc
     3121 cgatcgcagc gcgatattca ggatgccctt cgcaagtcgc aggagctcgc ggcggaacag
     3181 aaggatgtcg cgtccgaagt caatcagctc gaggggcagc agggcaccga tcgccgggtg
     3241 aaggcccagc agctcgcgct gcgcaagttc acgatggaca acaagcttgg tgagctgcag
     3301 tccgatctgg agaagctggc caacgacgcg cgcaaggacg agaaggatgc gtcccgcaag
     3361 ctcgacgagg ccgccggcag catccgcgac aagcgcgtga gggaaaagat ccgctactcg
     3421 cggagcgtgc tgcagggtgc cacgccgcag tccgcgccgg ggatggaaga agacatctcc
     3481 gcgaacctcg acgcgctgga aaagaagatc gccgaggcgc agggcgccat cggcaacaag
     3541 tcgaagcagg atgggctcgc gaaggcggcc gaccaggcgc gcgatctcgt gcgcgggatg
     3601 gaatcgctcg atcagcggat tcgcgatcgg gcgaatcagc aggcgcgcaa cggccagcag
     3661 cagcaaggca aggaaggcga gcaaggcaaa gagggccagc aagccaaaga gggccagcaa
     3721 ggcaaagagg gccagcaagg caaagaaggc cagcaaggca aagagggcca gcaaggtaaa
     3781 gaggggcagc agggcaaaga gggccagcaa ggcaaaggcg gccagcaagg taaagaaggc
     3841 cagcagggcc aggcccaggg gcaagagggc cagcagggcg gcgcgcgcgg aggggacgcc
     3901 aatcgcgcac cgcaagtcgg cgcctatggc ggcgacgcgc gtcccgacgg ggcgaactac
     3961 ggcgggcggt tcaccgctga cgacgtgcgg cagttcacgc ggcagttcca ggagctcaat
     4021 cgcgacgcgc agcagctgcg tcagcagctt caggcggccg gcatcaatcc ggcggatctc
     4081 gacgcggtca tgcgcggcct gacacagggg cagcaggcga gcatctacga cgatccgaac
     4141 gcgctcgcca agctccaggc ggcggcgctc gagcggttga agcagtttga attcaacctg
     4201 cggaagaagc tcgagaccgg cggcgagtcc ctcgcgctct cggggtcgga tgaagtgccg
     4261 gcgggcttcc ggcaggcgat cgaggagtac tatcgggcgc tcgcaaagag agcgacgaag
     4321 taggccgcga gccgctgctg aattgcagat ttcagattgc agatcgcaga ttcatcttct
     4381 gcaattcaat ctgaaatctg caatcctcaa tctgcaatct caccgtacca tgtcggggcg
     4441 tagcgcagcc tggtagcgca tctgctttgg gagcagaggg tcgctggttc gaatccagtc
     4501 gccccgacca cttctcttcg cctcgtacgt ggtgtcttag cctcgtacgt gtcttagcct
     4561 cgtacgtggt gtccggtttc cgcctccgct gaagcttcgg cgagactcgc cgtagcctta
     4621 gcgaaggcgg ttagccggac cgtaggacca caccagcgac agcacggcca gcgcgagggc
     4681 cgtcagaccg gacatc
//
LOCUS       DASYJA010000228         4868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1577696, whole genome shotgun sequence.
ACCESSION   DASYJA010000228 DASYJA010000000
VERSION     DASYJA010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577696"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VGO02_04845"
     CDS             <1..366
                     /locus_tag="VGO02_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204295.1"
                     /note="ligates
                     L-alanyl-gamma-D-glutamyl-meso-diaminopimelate to
                     UDP-N-acetylmuramic acid for reincorporation into
                     peptidoglycan; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEV7477049.1"
                     /translation="HPTAIRTTLDGLRQKVGKARVLAVLEPRSNTMKRGLMKDDLPGS
                     LEHADRIFVYSAGLGWDAPAVFASLGQRARCFADLEGLIAAIAAEARSGDHVLVMSNG
                     GFGGIHKRLLDRIAREAAA"
     gene            363..929
                     /locus_tag="VGO02_04850"
     CDS             363..929
                     /locus_tag="VGO02_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HEV7477050.1"
                     /translation="MIVYLHGFNSSPQSHKAQVLGRYMEEEGVGEEYACPALPPLAAQ
                     AIRTIENLTAAKSGVCFVGSSLGGYYATYLAEKHDASAVLINPAIDPHVGLRAYLGPQ
                     KNLHTGEPYTLTEAHLDQWEKLDVPRITPGRYLLIVETGDEVLDYRRAVARYAGAEQR
                     VVPGGDHSLQSFPEHLGRILEFARATAL"
     gene            913..2799
                     /locus_tag="VGO02_04855"
     CDS             913..2799
                     /locus_tag="VGO02_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEV7477051.1"
                     /translation="MQLLYEEDGELKVGAVLAQAPASFQVESPHGRRSKIKAASVLLS
                     FERPAGAELLAAAHEFAGGLDAAFLWECSAGREFGFQDLARDYVGREPTPMEAAGVLV
                     KLHSAPIYFYRRGRGRFQAAPEETLKLALAGLEKKKRLQESIAAWAERLVRYDCPPEI
                     AALKDELLYAPDRNKPETKAFEQACKEAGLSPARLLERCGVLTDAHEFHLRGFLHEFF
                     PRGTQFPPHDLPPLPADLPLARTAAFSLDDIGTTEIDDAFSVERVRPGELRIGVHIAA
                     PALGFAPGSPLGAIARDRLSTAYMPGRKFTMLPEDVIERFSLDHGGERPAISLYIDVA
                     EEDGTVRGHHSRLERVPVAANLRHAQYDVLNDTFESGGRAGLAFEEELRTLWRFATAL
                     EARRGRPSAGLATLDYSFHVEAGRVRIVPRKRGAPLDKLVAELMILANSTWGELLAER
                     DVAAIYRVQSVGKVRLSVHPEAHEGLGVSCYAWMTSPLRRYVDLVNQWQLAAAIGGSR
                     AAFARNSDALLGALRAFEVTYAGYDEHQRAMESYWCLRWLQQEGVTRIEAAVLRENLV
                     RLAGLPFTTRVPSLPETAPGTRVRLELKEIDLFERTAALIYRETLGEGAAVEDVTVDP
                     GQKA"
     gene            2831..3700
                     /locus_tag="VGO02_04860"
     CDS             2831..3700
                     /locus_tag="VGO02_04860"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEV7477052.1"
                     /translation="MYFTIEGRDRMLGTALGLSIAVHAVALGVHFRFPDAIRWQPPLQ
                     LEVVLVNAKTRERPVQADALAQANLDRGGDVDQNRRAKTPLPVTEPRNPGKDLTVAQR
                     RRQQLEMQQQQLLAQQKEVERRLAEKPRPARADEAANQPSGRDLADLSLAAMRLQAQI
                     DRQIEEYQKRPRKKFIGARAAEYRFAQYEEDWRMKVERIGSLNYPAEARGKYYGNLRL
                     TVTIRADGTVESIELDRSSGLKVLDAAAFRIVKMATPFAAFPADIRRDTDLLVITRTW
                     FFGQGDKIWTESQ"
     gene            3706..4503
                     /gene="aroE"
                     /locus_tag="VGO02_04865"
     CDS             3706..4503
                     /gene="aroE"
                     /locus_tag="VGO02_04865"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEV7477053.1"
                     /translation="MSERYAVIGHPVAHSKSPSIHAEFARATGQDIEYRRIEVARDGF
                     RRAAEEFRAAGGKGLNVTLPFKNEAFRYCGKASERARVAEAVNTLAFKEPVFGDNTDG
                     VGLVRDLSRNLHYDLRSSRALLMGAGGAAQAVVGALREAGVAQLVIVNRTVAKAQALA
                     ARFAGTRGCGYEDLGDESFGLIINATSASLLDAAPPVPALAFGRAALAYDMVYGRETP
                     FLAAARALGARTADGLGMLVEQAAESFFVWRGVRPETALVLRKLRGG"
     gene            4493..>4868
                     /locus_tag="VGO02_04870"
     CDS             4493..>4868
                     /locus_tag="VGO02_04870"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEV7477054.1"
                     /translation="MAARRGGTLRVLVALWKGFCYSLGALVLAFLLAQLWFFGNIVYL
                     SHYDPGSTAFMEKRLEILRAKKPGVQLRRTWAPYERISVQLKRAVVAAEDAKFLDHEG
                     FDWEAIQTAMTKNEQRGKVVAGA"
BASE COUNT          779 a         1646 c         1676 g          767 t
ORIGIN      
        1 cacccgaccg cgatccgcac cacgctcgac ggcctgcggc aaaaagtcgg caaagcgcgg
       61 gtgttggccg tgctcgaacc gcgctccaat actatgaagc gcgggctgat gaaggatgac
      121 ctgcccggca gcctggagca tgcggaccgt atcttcgttt acagcgccgg gttgggctgg
      181 gatgcaccgg cggtgtttgc ctcgctcggg caaagggcgc gctgcttcgc ggacctcgag
      241 ggactgatcg ccgcgatcgc cgccgaagcc cgcagcggcg accacgttct ggtcatgtcg
      301 aacggtggct tcggcggcat tcacaagcgg ctgctggatc gcattgcccg ggaagccgct
      361 gcgtgatcgt ttacctgcac ggtttcaact cctcgccgca gtcgcacaag gcccaggtac
      421 tgggccgcta catggaagag gagggggtag gcgaggagta cgcctgcccg gcgctgccgc
      481 cgctcgctgc gcaggcaatc cgcacgatcg agaacctgac cgcggcgaag tccggcgtct
      541 gctttgtcgg ctcttcgctc ggcggctact acgccaccta ccttgccgag aaacacgacg
      601 ccagcgcggt gctgatcaac ccggccatcg atccgcacgt cgggctgcgc gcctatctcg
      661 gcccgcagaa gaatcttcac accggcgagc cgtacaccct caccgaggct cacctcgatc
      721 aatgggaaaa gctggatgtg ccgcgcatca cgcccggccg ctacctgctg atcgtggaga
      781 cgggcgacga ggtgctcgac taccgccgcg cggtcgcgcg ctacgcgggc gcagagcagc
      841 gcgtggtgcc cggcggcgac cacagcctgc agagcttccc ggagcatctg ggccgcatcc
      901 tcgagttcgc ccgtgcaact gctctatgag gaagatggcg agctgaaggt cggggcggtc
      961 ctcgcccagg cgccggcttc gttccaggtg gaaagccccc acggccggcg ctcgaagatc
     1021 aaggcggcga gtgtgcttct ctccttcgag cggcctgctg gggcggagct gcttgctgcg
     1081 gcgcacgaat tcgccggcgg gctcgacgcc gcgttcctct gggaatgcag cgccgggcgc
     1141 gagttcggtt tccaggatct ggcgcgcgac tatgttgggc gcgagcccac gccgatggag
     1201 gcggccggcg tgctggtcaa gctgcactcc gcgcccatct acttctaccg acgcggcagg
     1261 ggccgcttcc aggccgcgcc cgaggaaacg ctcaagctcg cgctggcggg actggagaag
     1321 aaaaagcgct tgcaggagag cattgcggcg tgggccgagc gcctcgtgcg ctacgactgt
     1381 ccgccggaga tcgcggcgct gaaggacgaa ctgctgtacg cgccggaccg caacaagccc
     1441 gagacgaagg cattcgagca ggcgtgcaag gaggcggggc tttcgccggc gcggctgctc
     1501 gagcgctgcg gcgtgctcac cgacgcccat gagtttcacc tgcgcggctt cctgcatgaa
     1561 ttctttccgc gcggcacgca gtttcccccg cacgacctgc cgccgctgcc cgccgacctg
     1621 ccgctcgcca ggaccgccgc cttcagcctg gacgacatcg gcaccaccga gattgacgac
     1681 gccttctcgg ttgagcgggt gcggcccggc gagctgcgca tcggcgtaca cattgcggcg
     1741 ccggcgctgg gctttgcgcc gggctcgccg ctaggcgcga tcgcgcgcga tcgcctgtcg
     1801 accgcctaca tgccggggcg caagttcacc atgctgccgg aggacgtgat cgagcgcttt
     1861 tcgctcgacc acggtggcga gcgcccggcg atctcgcttt acatcgacgt tgccgaggag
     1921 gacggcacgg tgcgcgggca tcactcgcgg ctcgagcggg tgccggtggc cgccaacctg
     1981 aggcacgcgc agtacgacgt gctgaacgac acgtttgaat cgggcggccg cgccgggctc
     2041 gccttcgagg aggagctgcg cacgctctgg cgcttcgcca ctgcgctgga ggcgcgccgc
     2101 ggcaggccga gcgccggcct cgccaccctc gattattcat tccatgtcga ggcgggcagg
     2161 gtgcgcatcg tgccgcgcaa gcgcggcgcg ccgctcgaca agctggtcgc ggagctgatg
     2221 atcctcgcca actcgacctg gggggagttg ctggccgagc gcgatgtcgc cgcgatctac
     2281 cgggtgcagt cggtcggcaa ggtgcgcctg tcggtgcatc ccgaagcgca cgaaggcctc
     2341 ggcgtctcgt gctatgcctg gatgacctcg cccctgcgcc gctatgtcga cctcgtcaac
     2401 caatggcagc tcgccgccgc tatcggcggc agccgtgctg cgtttgcgcg caattccgat
     2461 gcgctgctcg gcgcgctgcg cgcgttcgag gtgacctacg cgggctacga cgagcaccag
     2521 cgcgcgatgg agagctattg gtgcctgcgc tggttgcagc aggaaggcgt cacccggatc
     2581 gaggcggccg tcctgcgcga gaacctcgtg cgccttgcag gcctgccgtt cacgacgcgc
     2641 gtgccgtcgc tgcccgagac tgctccggga acgcgggtgc ggctggaact caaggagatc
     2701 gacctgttcg aacgcacggc cgccttgatc taccgtgaga cactaggtga gggcgcggca
     2761 gtggaggatg tcacggtgga ccctggtcaa aaagcctaaa atatgaaagg cttgtggtat
     2821 tgaagtgaga atgtacttca caatcgaggg ccgcgatcgg atgctgggca ccgcgctcgg
     2881 gctctccatt gccgtccacg ctgtggccct cggagtgcac ttcaggtttc ccgacgcgat
     2941 tcgctggcag ccgccgctgc agctcgaggt cgtgctggtc aacgcaaaga cgcgcgagcg
     3001 cccggtgcag gccgacgcgc tcgcccaggc gaacctcgac cgcggcggcg atgtcgacca
     3061 gaaccgccgc gccaagacgc cgctgccggt gacggagccg cgcaatccgg gcaaggacct
     3121 gaccgtcgcc cagcgccgcc ggcagcagct cgagatgcag cagcagcagt tgctggcgca
     3181 gcaaaaggaa gtggagaggc gcctggccga gaagccgcgc ccggcaaggg ccgacgaagc
     3241 cgccaaccag cccagcggcc gcgacctcgc cgacctgtcg cttgccgcca tgcgcctgca
     3301 ggcgcagatc gaccgccaga tcgaggagta ccagaagcgg ccgcgcaaga aattcatcgg
     3361 cgcgcgcgct gccgagtacc gcttcgcgca atacgaagag gactggcgca tgaaggtcga
     3421 gcgcatcggc agcctcaact acccggccga agcgcgcggc aagtactacg gcaacctaag
     3481 gctgacggtc accatccgtg cagacggcac cgtcgagtcg atcgagctcg accgctcctc
     3541 gggtctcaag gtgctcgacg ccgccgcctt ccgcatcgtc aagatggcca cgcccttcgc
     3601 ggccttcccc gccgacatcc gccgcgacac cgacctgctg gtcatcaccc gcacttggtt
     3661 cttcggtcag ggcgacaaga tctggactga gtcccagtag ctttcatgag cgagcgctat
     3721 gcggtgatcg gccatcccgt ggcgcactcg aagtcgccgt cgatccacgc cgaattcgcg
     3781 cgcgccaccg gccaggacat cgagtaccgc cgcatcgagg tcgcgcgcga cggcttccgg
     3841 cgcgcggcgg aggagtttcg cgccgccggc ggcaagggcc tgaatgtcac gctgccgttc
     3901 aagaacgaag ccttccgcta ctgcggcaag gcgagcgagc gggcgcgcgt cgccgaggcg
     3961 gtcaacacgc tcgccttcaa ggagccggtt tttggcgaca acaccgacgg cgtcgggctg
     4021 gttcgcgacc tgagccgcaa tctgcactac gatctgcgca gctcgcgcgc cctgttgatg
     4081 ggcgcgggcg gcgcggcgca ggcggtggtc ggcgcattgc gcgaagccgg cgtcgcccag
     4141 ctggtgatcg tcaatcgcac cgtggcgaag gcgcaggcgc tcgccgcgcg cttcgccggg
     4201 acgcgcggct gcggctacga agacctgggc gatgagtcgt tcggcctgat catcaacgcc
     4261 acctcggcca gcctgctgga cgcagccccg cccgtgccag ccctggcgtt cgggcgggct
     4321 gcccttgcct acgacatggt ctacggacgc gaaacgccgt ttctcgctgc ggcacgggcc
     4381 ctcggagcgc gcaccgcgga cgggcttggg atgctggtcg agcaggccgc ggaatcgttc
     4441 ttcgtctggc gcggcgtgcg tcccgaaacc gcgctggtgc tgcggaagct gcgtggcggc
     4501 taggcgcggc ggcacgctgc gcgtgctggt cgcgctgtgg aagggctttt gctacagcct
     4561 gggcgcgctg gtcctcgcct tcctgctcgc gcagctctgg tttttcggca acatcgtcta
     4621 cctgtcgcac tatgatccag ggtcgaccgc cttcatggag aagcggctcg agatcctgcg
     4681 cgcgaagaag cccggcgttc agttgcgtcg cacctgggcg ccctacgagc ggatctcggt
     4741 gcagctcaag cgcgcagtgg tcgccgccga ggacgccaag ttcctcgacc atgagggatt
     4801 cgattgggag gcgatccaga cggcgatgac caagaatgag cagcgcggga aagtagtcgc
     4861 cggggcct
//
LOCUS       DASYJA010000229         2727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_34182, whole genome shotgun sequence.
ACCESSION   DASYJA010000229 DASYJA010000000
VERSION     DASYJA010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34182"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VGO02_04875"
     CDS             <1..351
                     /locus_tag="VGO02_04875"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516191.1"
                     /note="catalyzes the formation of 2-C-methyl-D-erythritol
                     4-phosphate from 1-deoxy-D-xylulose-5-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEV7477055.1"
                     /translation="ALAWPERMESGVPPLDLSLVKNLSFERPDPARFPCLSLAYAALL
                     AGGTAPAVLNAANEVAVHAFLGGRLPFTDIGGVIETTLARCEMRSAENLPAVLAADAQ
                     ARRTATGLVMAQAA"
     gene            348..1703
                     /gene="rseP"
                     /locus_tag="VGO02_04880"
     CDS             348..1703
                     /gene="rseP"
                     /locus_tag="VGO02_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEV7477056.1"
                     /translation="MSLLVDYVLPFIVALGVLIVVHEYGHYLVARLCGVKVLRFSVGF
                     GRALLVIRTGKDQTEWVIAAIPFGGYVKMLDEREGPVAPQEAARAFNRQSVGKRFAIV
                     LAGPLFNFAFAILLYAGLFMHGIPEPRPMVAEPPAGSAAAAAGLHAGDLVRAIDGDPV
                     VTWQDLRWRVLKAALQREAVQLETLSERGHIQSSTLDLRSFPADEVEKDVLERVGLNL
                     HRPPLEPVLGRVDAGPGQRAGLAPGDLVTHIDGAPVNTWMEVVRTIQQSADKQLAFGI
                     VREGKPLSVQVTPEAARDRDGNTIGRVGIGVRDSSGLFLQVRYGLGASLLKAVDKTVE
                     ISLFSLKMLGKMLLGQVSWKHLSGPVTIADFAGQSAQMGLPYFLNFLALISISLGVLN
                     LLPIPLLDGGHLMYYLIEIAKGKPVSERVMELGQRVGLALLLVMMAFAFYNDLNRLLT
                     G"
     gene            1704..>2727
                     /gene="bamA"
                     /locus_tag="VGO02_04885"
     CDS             1704..>2727
                     /gene="bamA"
                     /locus_tag="VGO02_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEV7477057.1"
                     /translation="MLRSSFIAAVFASCLAVAVADAGAQTAPGTFKPFVVKDIRVEGL
                     QRTEPGTVFSYLPIKVGETMDDQRAQRALRALFATGFFQDVRLEMENDVLVVLVTERP
                     AIAQIDFVGMKEFEPEVVKKVLRENGLAEGRIFDRSVLEQAEQELKKQYLSKGMYAVE
                     VQTTVTPLERNRVGINIGVTEGEVAKIQGINIVGAEAFPESELLGQFTLRTPGWLTWY
                     TKADRYARDKLSADIEVLRSYYLNRGYLDFKIDSTQVAITPDRKEIYITINLAEGDKY
                     TVTDVQLGGQLLLPREELEKLVQLKAGEVFSREKLNASTKAIADRLGNEGYAFANANA
                     IPRIDKD"
BASE COUNT          448 a          915 c          925 g          439 t
ORIGIN      
        1 gcgctcgcct ggcccgaacg catggagtcc ggcgtgccgc ccctggatct gtccttggtg
       61 aagaatcttt cgttcgagcg gcccgacccg gcacgctttc cctgcctgtc gctcgcctac
      121 gccgcgctgc tcgccggcgg cacggcaccg gcggtgctga acgccgcgaa cgaggtcgcc
      181 gtgcacgcgt tcctcggcgg gcggctgccg ttcaccgata tcggcggggt aatcgagacg
      241 acgctcgcca gatgtgaaat gcgctccgct gaaaatcttc ccgccgtgct cgcggccgat
      301 gcccaggcgc ggcgcaccgc gaccggcctg gtgatggcgc aagccgcatg agcctcctcg
      361 tggattacgt ccttcccttc atcgtcgccc tcggcgtgct catcgtcgtg cacgagtacg
      421 ggcactacct ggtggcgcgg ctgtgcgggg tgaaggtgct gcgtttctcg gttggctttg
      481 gcagggcatt acttgtaata cgaactggca aggatcagac ggagtgggtg atcgcggcga
      541 ttccattcgg cggctacgtc aagatgctcg acgagcgcga agggccggtg gcgccgcagg
      601 aagcggcgcg cgccttcaac cgccagagcg tcggcaagcg cttcgcaatc gtgctggccg
      661 ggccgctgtt caatttcgcc ttcgccatcc tgctgtacgc ggggctgttc atgcacggca
      721 tacccgagcc caggccgatg gtcgcggagc cgcccgccgg cagcgcggcc gccgccgccg
      781 ggctgcacgc gggggacctg gtgcgcgcga tcgacggtga cccggtcgtc acctggcagg
      841 acctgcgctg gcgcgtgctg aaggccgcgc tgcagcgtga ggcggtgcag ctcgagacgc
      901 tcagcgagcg cggccacatc cagagctcga cgctcgacct gcgcagcttc ccggccgatg
      961 aagtggagaa ggacgtcctc gagcgcgtcg gcctcaacct gcaccggccg ccgctcgagc
     1021 cggtcctcgg ccgggtcgat gcgggcccgg ggcagcgcgc ggggctcgcg cccggggacc
     1081 tggtcacgca catcgacggc gcaccggtga acacctggat ggaggtggtg cgcaccatcc
     1141 agcaaagcgc cgacaagcag ctcgccttcg gcatcgtgcg cgagggcaag ccgctcagcg
     1201 tccaggtgac gcccgaggcg gcgcgcgacc gcgacggcaa cacgatcggc cgggtcggca
     1261 tcggcgtgcg cgacagcagc ggcctgttcc tgcaggtgcg ctatggactg ggcgcgagcc
     1321 tgctgaaagc cgtcgacaag accgtcgaga tttcgctctt cagcctgaag atgctcggca
     1381 agatgctgct cggccaggtg tcgtggaagc atctgtccgg gccggtcacc atcgccgact
     1441 tcgccggcca gtcggcgcag atggggctgc cttacttcct caatttcctg gctctgatca
     1501 gcatcagcct cggcgtgctc aacctgctgc cgatcccgct gctcgacggc ggacacctga
     1561 tgtactatct gatcgagatt gcaaaaggca agcccgtgtc cgagcgcgtg atggagctcg
     1621 ggcagcgcgt tggtctggcg ctgctcctgg taatgatggc ttttgctttc tacaacgacc
     1681 tgaaccgcct cctcaccggc tgaatgctcc gcagcagttt catcgccgcg gtcttcgcgt
     1741 cgtgcctggc cgtggcggtc gcggacgccg gcgcccagac ggcgccggga acattcaagc
     1801 ccttcgtcgt caaggacatc cgggtcgagg gcctgcagcg caccgagccg ggcacggtgt
     1861 tcagctacct gccgatcaag gtcggcgaga cgatggacga ccagcgggcg cagcgggcct
     1921 tgcgcgccct gttcgccacc ggtttcttcc aggacgtgcg cctcgagatg gagaacgacg
     1981 tcctcgtcgt gctggtcacc gagcgcccgg cgatcgcgca gatcgacttc gtcggcatga
     2041 aggagttcga gcccgaggtg gtgaagaagg tgctgcgcga gaacggcctg gccgagggac
     2101 gcatcttcga ccgctcggtg ctcgagcagg ccgagcagga gctgaagaag cagtacctgt
     2161 ccaagggcat gtacgcggtc gaggtgcaga ccacggtgac cccgctcgag cgcaaccgcg
     2221 tcggcatcaa catcggcgtc accgagggcg aggtcgccaa gatccagggc atcaacatcg
     2281 tcggcgccga ggcgttcccg gagagcgagc tgctggggca gttcaccctg cgcaccccgg
     2341 gctggctgac ctggtacacc aaggccgacc gctacgcgcg cgacaagctc tcggccgaca
     2401 tcgaggtgct gcgctcgtac tacctgaacc gcggctatct cgacttcaag atcgattcga
     2461 cgcaggtggc gatcaccccc gaccgcaagg aaatctacat caccatcaac ctcgcggagg
     2521 gggacaagta cacggtgacc gacgtgcagc tcggcggcca gctgctgctg ccgcgcgagg
     2581 agctggagaa gctggtgcag ctcaaggccg gggaggtgtt ctcccgcgag aagctcaacg
     2641 ccagcaccaa ggccatcgcc gaccgcctcg gcaacgaggg ctacgccttc gccaacgcca
     2701 acgcgatccc gcgcatcgac aaggaca
//
LOCUS       DASYJA010000230         2928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2121809, whole genome shotgun sequence.
ACCESSION   DASYJA010000230 DASYJA010000000
VERSION     DASYJA010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2121809"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VGO02_04890"
     CDS             complement(<1..615)
                     /locus_tag="VGO02_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477058.1"
                     /translation="MGKNVGRSIAFAMFAAALSLAVPSAHAAGLGKITVLSSLGQPLL
                     AEIEIVQLQAGEEEGLTARLPSSDAFIAAGIEPTAVLNGLRFNIQRRNNRPVLRVTSA
                     QPVNEPFLEMLVELQWATGRLVREYTLLLDPVEYKGQQQAIAAAPVAKPAPAEMKPAP
                     AAPETKPEPAAPAPEAAKPEASPQAAVEAKPEAAAAAEAKPEAKP"
     gene            complement(694..1827)
                     /gene="asd"
                     /locus_tag="VGO02_04895"
     CDS             complement(694..1827)
                     /gene="asd"
                     /locus_tag="VGO02_04895"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEV7477059.1"
                     /translation="MRVGIVGWRGMVGSVLVQRMREERDFDHLEPVFFSTSQAGGKGP
                     AIGKDTAPVKDAKDFAALKALPVIVSCQGGDYTNQVYPELRKQGWKGYWIDAASALRM
                     QDDAVIILDPVNMQVIKDSLSKNIKNYIGGNCTVSLMLMGLAGLFQRDEIEWMTSMTY
                     QAASGAGAANMRELVAQMAQVGDSARALLEDPASAILDIDRAVTDSIRARGLPKESFG
                     YPLAASLLPWIDKDMGNGQSREEWKGQAETNKILGRNGKSGGSAIPVDGVCVRVGAMR
                     CHSQALTIKLRRAVPLDEIEGMLADAHDWVKVVPNRREESLAELTPAAVTGKLSVPVG
                     RLRKLPMGNDYLTAFTVGDQLLWGAAEPLRRMVRILLESGRIT"
     gene            complement(1841..2902)
                     /gene="leuB"
                     /locus_tag="VGO02_04900"
     CDS             complement(1841..2902)
                     /gene="leuB"
                     /locus_tag="VGO02_04900"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEV7477060.1"
                     /translation="MKIALLAGDGIGPEILVEAKKVLDVLRGEGVKIETEEALVGGAA
                     YDAHGDPLPQATLDLVKASDAVLFGAVGGPQYDKLARDKRPEKAILGLRRECDFFANL
                     RPATVFPELADASTLKPEIVSGLDIMIVRELTGDIYFGEPRSVTGAKGARVGINTMRY
                     TEAEIRRILHAGFRTARQRGGRLCSVDKMNVLETSQLWRDLAEEIAPEYPEVALSHML
                     VDNCAMQLVRNPRQFDVIVTGNLFGDILSDEASMLTGSIGMLPSASLDANGKGLYEPI
                     HGTAPDIAGKDLANPLATILSAAMMLRFSLAQDKAAERIEAAVRKVLREGYRTADIHT
                     PGGRKVGTAEMGDAVLRAL"
BASE COUNT          440 a          979 c          952 g          557 t
ORIGIN      
        1 tggcttggct tccggcttcg cttcggctgc ggctgcggct tccggcttgg cctcgactgc
       61 ggcttgcggg ctcgcctcgg gcttggccgc ttccggcgcc ggcgccgcgg gctcgggttt
      121 ggtctccggt gcggcaggcg ccggcttcat ttcagccggc gccggcttgg ccaccggcgc
      181 tgcggcgatc gcctgttgct gtcccttgta ctccaccggg tcgagcagca aggtgtattc
      241 gcgaaccagg cggccggtcg cccactgcag ctcgaccagc atttcgagga acggctcgtt
      301 caccggctgc gcgctggtga ctctgagaac cgggcggttg ttgcggcgct ggatgttgaa
      361 gcgcaggccg ttcagtaccg cggtcggctc gataccggcg gcgatgaagg catcgctcga
      421 aggcagcctg gccgtgagac cttcttcttc gcccgcctga agctggacga tttcgatctc
      481 ggcgagcaac ggttggccga gagaggacag cacggtgatc ttgccgaggc cggcagcgtg
      541 cgcggacggc accgcaagcg acagcgcggc cgcgaacatc gcgaaggcga tcgatctacc
      601 cacgtttttc cccaccctgt cttcttagct gctggaccaa tcaccttaac atcatggtct
      661 tactgctgca agttgagcgt ttttctcact tttttacgtt atccgcccgg actccagaag
      721 gattctgacc atgcggcgca ggggttcggc ggccccccag aggagctggt cgccgacggt
      781 aaaggccgtc aggtagtcat tccccatcgg gagcttgcgc agccggccca ccgggaccga
      841 cagcttgcca gtcacggcgg ccggggtgag ctcggcgagc gactcctcgc gccggttcgg
      901 caccaccttc acccagtcat gcgcgtcggc cagcatgccc tcgatctcgt cgagcggcac
      961 ggcccggcgc agcttgatgg tcagcgcctg gctgtggcag cgcatggcgc ccacccgcac
     1021 gcagacgcca tcgacaggga tcgcggagcc gccgcttttt ccattcctgc cgaggatctt
     1081 gttggtctcc gcctggccct tccactcttc gcggctctgg ccgtttccca tgtccttgtc
     1141 gatccagggc agcaggctgg cggcgagcgg gtagccgaag ctctccttcg gcaggccgcg
     1201 cgcgcggatc gaatcggtca ccgcgcggtc gatgtcgagg atggccgagg ccggatcctc
     1261 gagcagcgct cttgcgctat cgccgacctg cgccatctgc gcgaccagct cgcgcatgtt
     1321 ggccgcgccg gcgcccgagg cggcctggta cgtcatgctg gtcatccact cgatttcgtc
     1381 gcgctggaac agtccggcga ggcccatcag catcaggctc accgtgcagt tgccgccgat
     1441 atagtttttt atatttttag aaagagaatc ctttattacc tgcatgttga ccgggtcgag
     1501 gatgatgacg gcgtcgtcct gcatgcgcag ggcgctggcc gcgtcgatcc agtagccctt
     1561 ccacccctgc ttgcgcagct ccggatagac ctggttcgtg tagtcgccgc cctggcacga
     1621 gacgatcacg ggcaatgcct tcagcgcggc gaagtccttc gcgtccttga ccggcgccgt
     1681 gtcttttcca atggccggac ctttgccccc cgcctgcgaa gtggagaaaa acaccggctc
     1741 gagatgatcg aagtcgcgct cctcgcgcat gcgctgcacc aggaccgagc ccaccattcc
     1801 gcgccaaccg acgatgccga ctctcaacat aaacagcagt ttacagcgct cggaggaccg
     1861 cgtcgcccat ttccgcggta ccgaccttcc ggccgccggg cgtatggatg tcggcggtgc
     1921 ggtagccctc gcgcagcact ttgcgcaccg cggcctcgat ccgctccgcg gctttgtcct
     1981 gcgcgagcga gaagcgaagc atcatcgccg cagaaaggat cgttgccagc gggttggcga
     2041 ggtctttgcc cgcgatgtcg ggcgcggtgc catggatcgg ctcgtagaga cccttgccat
     2101 tggcatcgag cgacgccgac ggcagcatgc cgatcgagcc ggtcagcatc gaagcctcgt
     2161 cggaaaggat gtcgccgaaa aggttgccgg tcacgatcac gtcgaattgc ctggggttgc
     2221 gcaccagctg catggcgcaa ttgtccacga gcatgtgcga aagcgccacc tcggggtact
     2281 ccggggcgat ctcctcggcg aggtcgcgcc agagctgcga agtctcgagc acgttcatct
     2341 tgtccaccga gcagagtcgc ccgccgcgtt ggcgtgcggt cctgaagccc gcgtgcagga
     2401 tgcggcggat ctccgcctcc gtgtagcgca tggtgttgat gccgacgcgc gcgcctttcg
     2461 cgcccgtgac gctgcgcggc tcgccgaagt agatatcgcc ggtcagctcg cgcacgatca
     2521 tgatgtcgag gccgctcacg atttccggct tcagcgtcga ggcgtccgcg agctcgggga
     2581 acacggtcgc gggacgcagg ttggcgaaga agtcgcattc ccggcgcagg ccgaggatcg
     2641 ccttctccgg gcgcttgtcg cgcgcgagct tgtcgtactg cgggccgccg accgcgccga
     2701 agagcacggc gtcggaagcc ttgaccaggt cgagcgtggc ctgcgggagc ggatcgccgt
     2761 gcgcgtcgta ggccgcgcct ccgacgagcg cctcctcggt ctcgatcttc acgccctcgc
     2821 cgcgcaggac gtcgaggacc ttcttcgctt caacgaggat ttccgggccg atgccgtcgc
     2881 cggccagcaa ggcgatcttc atctacgaga agatccaggg ttcgctct
//
LOCUS       DASYJA010000231         2961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_397816, whole genome shotgun sequence.
ACCESSION   DASYJA010000231 DASYJA010000000
VERSION     DASYJA010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397816"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VGO02_04905"
     CDS             complement(<1..310)
                     /locus_tag="VGO02_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEV7477061.1"
                     /translation="MLFFAGGVLSETKDLDRRVAELSAELRCLVCQNQSIADSNADLA
                     VDLRRQIREQLGSGKSERDVVDFMVQRYGDFVLYRPPLKASTVLLWAGPFLFLGLGTF
                     I"
     gene            complement(327..854)
                     /locus_tag="VGO02_04910"
     CDS             complement(327..854)
                     /locus_tag="VGO02_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012358245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEV7477062.1"
                     /translation="MSSRAWVPAIFIVLLVLLAAGLTLNPREVPSPLVGKAAPPFELP
                     LLREPDKVFASKERLGSVWLLNVWASWCVSCRDEHPVLLEFSKSSALPLYGLNYKDKR
                     EDGLRWLATYGDPYRLSVVDAQGRIGIDYGVYGVPETYVIDKRGVIRYKQIGAVTREV
                     MVQKILPLVGQLARE"
     gene            complement(851..2779)
                     /locus_tag="VGO02_04915"
     CDS             complement(851..2779)
                     /locus_tag="VGO02_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEV7477063.1"
                     /translation="MIPELGQVALSLALALALFLSATSLYGAEKSNAAWISFARPSAQ
                     GLALLVAFAFGCLVHSFVVNDFSVAYVAMNSNSALPLHYRVAGAWGGHEGSLLLWALM
                     LGVWMSAVSLFSRHLPDEMTARVLGVMGLISAGFLAFMLFTSNPFDRMLPAAADGRDL
                     NPLLQDPGMVLHPPMLYMGYVGFSVAFSFAVAALLAGRLDATWARWSRPWTTVAWMFL
                     TVGIALGSGWAYYELGWGGWWFWDPVENASFMPWLCGTALIHSLAVTEKRGAFRSWTV
                     LLAIFAFALSLLGTFLVRSGVLTSVHAFAVDPKRGVFILALFALLVGGALALYAWRTA
                     RIGLGGGFSALSRESFLLANNVLLGAAAGTVFLGTLYPLALDALGAGKISVGPPYFET
                     VFVPLMAPALFLMGIGPLARWKRSSLPELAVLLRWALAVSLALGLLLPLAMGSWKPMV
                     GFGLSLALWVILSALVSLRKSLRQTRSHYGMVVAHIGVAVFVIGVTLVKGYEVEKDAR
                     MRPGERVEVAGYVFLLEGVANVAGPNYQAARAKVSVSRNGSPVSVLHPERRLYTVQQN
                     VMTEAAINTGLTRDLYVSLGDPLDGGAWLVKMQVKPFIDWIWGGCLIMALGGLLAASD
                     RRYRFALKKESILAEARA"
     gene            complement(2776..>2961)
                     /locus_tag="VGO02_04920"
     CDS             complement(2776..>2961)
                     /locus_tag="VGO02_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEV7477064.1"
                     /translation="FRGALPDLFREGKGVVAQGQLGADGVFRAHEVLAKHDENYMPPE
                     AKLAVEKAHEASKTLKP"
BASE COUNT          583 a         1017 c          973 g          388 t
ORIGIN      
        1 gaataaaagt cccgaggccg agaaacagga acggcccggc ccagagcagc acggtgctcg
       61 ccttgagcgg cgggcggtag agcacgaagt cgccgtagcg ctgcaccatg aagtcgacga
      121 catcgcgctc gctcttgccg ctgcccagct gctcgcggat ctgccggcgc aggtcgaccg
      181 ccaggtcggc gttggaatcg gcgatcgact ggttctggca gaccaggcag cgcagctcgg
      241 cggacagctc cgccacgcgc cggtcgagat ctttggtttc agaaagaacc ccaccggcga
      301 aaaacagcat caacaaaaaa caaaatctat tcacgagcga gctggccgac cagcggcagg
      361 atcttctgca ccatgacctc gcgcgtcacc gcgccgatct gcttgtagcg gatgacgccg
      421 cgcttatcga tgacgtaggt ttcgggcacg ccgtagacgc cgtagtcgat gccgatgcgc
      481 ccttgcgcat cgaccaccga cagccggtac gggtcgccgt aggtggcgag ccagcgcagg
      541 ccgtcctcgc gcttgtcctt gtagttgagg ccgtagagcg gcagcgcgct gcttttcgag
      601 aactccagaa gaacggggtg ctcgtcgcgg cacgacacgc accacgaagc ccacacgttg
      661 agcagccata cgctgcccag ccgctccttc gaggcgaaca ccttgtccgg ctcgcgcagc
      721 agcggcagct cgaacggcgg cgccgccttg ccgaccagcg gcgagggcac ctcgcgcgga
      781 ttgagcgtca ggccggcggc gagcagcacc agcagcacga tgaagatcgc cggcacccag
      841 gcgcgcgagc tcatgcgcgc gcttccgcca ggatggattc ttttttcaag gcaaaccggt
      901 agcggcggtc gctcgccgcc agcagtccgc cgagcgccat gatgaggcag ccgccccaga
      961 tccagtcgat gaacggcttg acctgcatct tgacgagcca cgcgccgccg tcgagcggat
     1021 cgcccagcga tacatacaaa tcgcgggtca gcccggtgtt gatcgccgcc tcggtcatga
     1081 cgttctgctg aacggtgtag aggcgcctct ccggatgcag cacgctcacc gggctgccgt
     1141 tgcgggaaac ggacaccttg gcgcgcgccg cctggtagtt gggcccggcg acgttcgcga
     1201 cgccctccag caggaagacg tagccggcga cctcgacccg ctcgcccggg cgcatgcggg
     1261 cgtccttctc gacctcgtag cccttcacca gcgtgacgcc gatcacgaac accgcgacgc
     1321 cgatgtgcgc caccaccatg ccgtagtgcg agcgcgtctg cctgaggctt tttctcaagg
     1381 agaccagcgc gctaagaatg acccacaggg caagagacag cccgaacccg accatcggct
     1441 tccagctgcc catcgccagc ggcagaagca gccccagcgc caggctgact gccagcgccc
     1501 agcgcagcag caccgcgagc tcgggcaagc tcgagcgctt ccagcgcgcg agcgggccga
     1561 tgcccatcag gaacagcgcc ggcgccatga gcggcacgaa taccgtctcg aagtacggcg
     1621 gcccgaccga gatcttgccg gcgccgagcg cgtcgagcgc cagcggatag agcgtgccga
     1681 ggaaaacggt gccggccgcc gcccccagca gcacgttatt tgccagcaag aaactttccc
     1741 ttgaaagcgc ggaaaaacca ccccctagac ctatgcgcgc ggtgcgccac gcgtacagcg
     1801 cgagcgcgcc gccgacgagg agcgcgaaga gtgccaggat gaagacgccg cgcttcgggt
     1861 cgacggcgaa agcgtgcacc gaggtgagca cgcccgagcg caccaggaag gtgccgagca
     1921 ggctgagcgc gaaggcgaag atggcgagca gcaccgtcca gctgcggaag gcgccgcgct
     1981 tctcggtcac ggcgagcgag tggatgagcg cggtgccgca cagccagggc atgaaggaag
     2041 cgttctccac cgggtcccag aaccaccagc cgccccagcc gagctcgtag taggcccagc
     2101 cgctgccaag agcgatgccg acggtgagga acatccaggc caccgtggtc cagggccgcg
     2161 accagcgcgc ccaggtcgcg tccaggcgcc cggcgagcag ggccgccacc gcgaacgaaa
     2221 acgccaccga gaacccgaca tagcccatgt acagcatcgg cgggtgcagc accatgcccg
     2281 ggtcctgcag cagcgggttg aggtcgcgcc cgtcggccgc cgccggcagc atgcggtcga
     2341 agggattcga ggtaaacagc atgaacgcga ggaagcccgc gctgatcagg cccatcacgc
     2401 cgagcacccg tgcagtcatc tcgtcgggca gatggcggct gaacaggctc accgcgctca
     2461 tccacacgcc gagcatcagc gcccacagca gcaacgagcc ctcatggccg ccccaggcgc
     2521 cggccacccg gtagtgcagc ggcagcgcgg aattcgagtt catcgcgacg taggcgacgg
     2581 agaagtcgtt gacgacgaag gaatggacca ggcagccgaa ggcgaaggcg acgagcagcg
     2641 ccaggccctg cgccgacggg cgcgcgaagc ttatccaggc ggcattggat ttttccgcgc
     2701 catacaggga cgtggccgaa agaaacagcg ccagggccaa ggcgagggaa agggcgacct
     2761 gtccgagctc ggggatcatg gcttcaatgt tttcgacgcc tcgtgcgcct tctccacggc
     2821 aagcttcgct tcgggcggca tgtagttctc atcgtgcttg gcgagcacct cgtgggcgcg
     2881 gaacacgccg tcggcgccga gttgcccctg cgccaccacg cccttgccct cgcggaacag
     2941 atccggcagc gcgccgcgga a
//
LOCUS       DASYJA010000232         2081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1396464, whole genome shotgun sequence.
ACCESSION   DASYJA010000232 DASYJA010000000
VERSION     DASYJA010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396464"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VGO02_04925"
     CDS             <1..382
                     /locus_tag="VGO02_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477065.1"
                     /translation="EPVMWEKYQLAKWTGGKFQSNTLIAEQKGAAADPKDYENPDGAF
                     SPHDNSIPALQRRGVVFLACHNAIWELAGTLIAAEVNPDELSHEALAAELTNHLIAGV
                     VLTPGMVGTLPELQRAGFNYATSN"
     gene            400..912
                     /locus_tag="VGO02_04930"
     CDS             400..912
                     /locus_tag="VGO02_04930"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEV7477066.1"
                     /translation="MSTYTERRFLPYTPAQLFELVAAVDRYPEFLPWCLAARIRFDKK
                     LQGTSNKASMVADLVIGFRMFRERYTSRVTLQPPQRIDVIYVEGPFRYLNNHWIFEPV
                     SRSADRPVDGTMLTFHIEFEFRSTLLQALVGGLFNEAVRRMVAAFEGRAKRLYGGQQV
                     RPGLRELARS"
     gene            955..2064
                     /locus_tag="VGO02_04935"
     CDS             955..2064
                     /locus_tag="VGO02_04935"
                     /inference="COORDINATES: protein motif:HMM:NF016089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TQO small subunit DoxD"
                     /protein_id="HEV7477067.1"
                     /translation="MTVVAINTAAPRAAAVTTLGERNWRMAAIALLSVRLIEGFIYWG
                     GGSRRFIYGPEKLDPSAPTWMANKLQSAMPGALLGTDHLIAFLLHHFWLLYPAVILFS
                     AAELIAGAALMAGLFTRLAALVSVGLSMALMLMFGWQGATCIDEWTMAACNLAMGATL
                     VLAGGAAYSLDNMLLRRNPGLAGRAWFRWAAGSLPLRLTDRAFRNFGLAVLAAVLAFI
                     IGTYSYYRGSVVTPFHGGPVSPTRHHFALSNGALLPDGSVKFQIYLDGGTPEAPADIM
                     KAELTGGDNKLLETWNTETLTRLPAESIHNDFAYNKFKAGPYGIVAEMGAMATIWLPP
                     QPNVHSPAGGNATLTLTDVNGRSFSLRLAGAGENS"
BASE COUNT          352 a          661 c          679 g          389 t
ORIGIN      
        1 cgagccggtg atgtgggaga aataccagct tgcgaagtgg actggaggca agttccagag
       61 caacacgctg atcgcggagc agaagggcgc ggccgccgat ccgaaagact acgagaatcc
      121 cgatggcgcc ttctcgccgc acgacaattc catcccggcg ttgcaacggc gcggcgtggt
      181 cttcctcgcc tgccacaacg ccatatggga gcttgcgggg acgctgatcg ccgcggaggt
      241 gaatcctgat gaattgtcgc acgaggcgct ggctgccgag ctgaccaatc acctgattgc
      301 cggtgtggtg ctcacacccg ggatggttgg aaccctgccc gagctgcagc gggccggctt
      361 caattacgca acctcgaact gaccgcgaag ctcaggacca tgtcgacata caccgagagg
      421 cgctttcttc cctacacgcc tgcgcaactt tttgagctgg tcgccgcggt cgatcgatat
      481 cccgagttcc tgccctggtg cctcgcggcg cgcatccgct tcgacaaaaa gctgcagggc
      541 acgtccaaca aggcgtcgat ggtggccgat ctcgtcatcg gcttccgcat gttccgcgag
      601 cgctacacgt cgcgcgttac cttgcagccg ccgcaacgca tcgacgtaat ttatgtcgaa
      661 ggcccgttcc ggtatttgaa caaccattgg attttcgagc cggtttcccg gagtgcggat
      721 cgcccggtcg acggcacgat gctcaccttc catatcgagt tcgagttccg ctcgacgctg
      781 ctgcaggcgc tggtaggcgg actgttcaac gaggcggtga gacggatggt tgccgccttc
      841 gaaggccgtg cgaagcggct ttacggtggc caacaggtgc ggcccgggtt gcgtgaactc
      901 gccagatcat gacggcagtc ggccgcctgg ctcatgctgc ggaaaggagt tgagatgacg
      961 gtcgtggcga tcaatacggc ggcgccgcgc gctgccgcag tgacgacgct cggtgagcgg
     1021 aactggcgca tggcggcgat cgcgctgctc tcggtgcggt tgatcgaggg cttcatctac
     1081 tggggtggag ggtcgcgccg ctttatctac gggccggaga agctcgatcc gagcgcgccc
     1141 acctggatgg caaacaagct ccagagtgcg atgcccggcg cgttgctcgg caccgatcac
     1201 ctgatcgcgt tcctgctgca tcacttctgg ctgctgtatc ccgccgtgat ccttttcagc
     1261 gctgccgagc tgatcgcggg cgcggcactg atggccgggc tgttcacgcg cctcgccgcg
     1321 ttggtctcgg tcgggctctc gatggcgctg atgctgatgt tcggttggca gggcgccacc
     1381 tgcatcgacg aatggacgat ggccgcctgc aatctggcga tgggcgccac cttggtgctc
     1441 gccggcggcg cagcctattc gctcgacaac atgctgctga ggcgcaatcc cggcctggcc
     1501 ggccgcgctt ggttccgctg ggccgccggg agcctgccgt tgcggctcac cgaccgggcg
     1561 ttccgcaatt tcgggctcgc ggtactggcg gcggttctgg ccttcatcat cggaacctac
     1621 agctactatc gcggctcggt cgtcacgccg ttccacggcg gcccggtcag cccgacgcgg
     1681 caccactttg cgctcagcaa cggcgcgctg ctgcccgatg gcagcgtgaa attccagatc
     1741 tatcttgacg gcggcacgcc cgaggcgccc gccgacatca tgaaggccga gctgaccggc
     1801 ggggacaaca agctgctcga aacctggaat accgagacgc tgacccggct gccggcggag
     1861 tccatccaca atgacttcgc ctacaacaag ttcaaggcgg gcccctacgg gatcgtggcc
     1921 gagatgggcg ccatggcgac catttggttg ccgccgcagc cgaacgttca ttcccctgcg
     1981 ggcgggaacg ccacattgac gctgaccgac gtgaacgggc gcagcttcag cctgaggctg
     2041 gccggtgcgg gtgagaattc ctgaatccag ggaacacttg g
//
LOCUS       DASYJA010000233         2134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3213744, whole genome shotgun sequence.
ACCESSION   DASYJA010000233 DASYJA010000000
VERSION     DASYJA010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3213744"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="VGO02_04940"
     CDS             <1..1039
                     /locus_tag="VGO02_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7477068.1"
                     /translation="RVTRRFGGAGSLPALHDLSLTIGRGEFVALLGPSGCGKTTALNC
                     IAGLLPLTAGGIWLDERRIDVLPAEKRGFGMVFQNYALFPHMTVRANVGFGLRMRGVG
                     AIEAAPRIDRALSLVQLGGHENKLPGQLSGGQQQRVALARAIVIEPPLVLMDEPLSNL
                     DAKLRLEMRSEIRRIHRELGRSTIYVTHDQDEALSLADRIIVMKDGEVQQIAAPAELY
                     TQPANVHVARFMGYRNVLELEVIQDVGGGRVILEGGGLRLEGVAKQPLARRALAAIRP
                     EDLTLGATASGNRIDGRVEAVEYCGRDSLVDVRTPQGALLHARAATSVAIGDAVQLGV
                     PASRVLAYPAG"
     gene            1047..1886
                     /locus_tag="VGO02_04945"
     CDS             1047..1886
                     /locus_tag="VGO02_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV7477069.1"
                     /translation="MRSPLLLVFPAVLFLLLFFIYPFFYGLWLSFQPLKGDWLANYAR
                     FFSDGHLWRTVITTFQLAIPATLINVGLALPIAFRMRQKSRYQRWVTTILVVPITLGT
                     VLIAEGMLAYLGPKGWLSQMLQLLHLYDGPIRLTHNYWGVLLSLVISGFPFAFLLILS
                     YITGIDPVLERAAATLGADAWRRFRHIYLPLLAPGLAITFCLAFVQAFSVFPSAVLVG
                     APAGSTRVISLAAYEAAFEQYDYPMASAVAMIMGFAQLAVVAAVLALRRGFYRGPVTG
                     GKG"
     gene            1883..>2134
                     /locus_tag="VGO02_04950"
     CDS             1883..>2134
                     /locus_tag="VGO02_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006476007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7477070.1"
                     /translation="MKAWLGRAWGALLLGSMALFVVNIGLMVAAVATNSFAKRWLGTW
                     LPEGYTTSWYAAAWKEFQLGWVLWVTVEVVGAVVLLSILI"
BASE COUNT          265 a          732 c          748 g          389 t
ORIGIN      
        1 gcgtgtcacg cgccgcttcg gcggggccgg cagcctgccg gcgctgcatg atctgtcgtt
       61 gacgatcggg cgcggcgagt tcgtcgcgct gctcggcccg tcgggctgcg gcaagaccac
      121 cgcgctcaac tgcatcgccg gcctgctgcc gctcaccgcc ggcggcatct ggctggacga
      181 gcggcgcatc gacgtgctgc cggcggagaa gcgcggcttc ggcatggtgt tccagaacta
      241 cgcgctgttc ccgcacatga cggtgcgcgc caacgtcggc ttcggcctga ggatgcgggg
      301 ggtcggcgcc atcgaggcgg cgcctcgtat cgaccgggcg ctctcgctgg tgcagctcgg
      361 cggccacgag aacaagctcc cagggcagct ttccggcggc cagcagcagc gcgtggcgct
      421 ggcgcgcgcc atcgtcatcg agccgccgct ggtgctgatg gacgagccgc tctcgaacct
      481 cgacgccaag ctgcgcctgg agatgcgctc cgagatccgc cgcatccacc gcgagctcgg
      541 ccgctcgacc atctacgtca cgcacgacca ggacgaagcg ctgtcgctcg ccgaccgcat
      601 catcgtgatg aaggacggcg aggtgcagca gatcgccgcc ccggcggagc tctacacgca
      661 gccggcaaac gtgcatgtcg cgcgcttcat gggctaccgc aacgtgctcg agctcgaggt
      721 gatccaggat gtcggcggcg gccgcgtcat cctggagggc ggcgggctgc gcctggaggg
      781 cgtggcgaag cagccgctcg cgcgccgcgc gctcgctgcg atccggccgg aggacctcac
      841 cctcggtgcc acggccagtg gcaaccgcat cgacggccgc gtcgaggcgg tcgagtactg
      901 cgggcgcgat tcgctggttg acgtgaggac gccgcagggc gcgctgctgc acgcgcgcgc
      961 ggcgacctcg gtggcgatcg gcgacgcggt gcagctcggc gtgcccgcgt cacgcgtgct
     1021 cgcgtacccg gcgggctgag caggccatgc gcagcccgct cctgctcgtc tttccggcgg
     1081 tgctgttcct gctgctgttc ttcatctacc cgttcttcta cgggttgtgg ctttcgtttc
     1141 agccgctgaa gggcgactgg ctggcgaatt acgccaggtt cttctccgac gggcatctct
     1201 ggcgcaccgt catcaccacc tttcagctgg cgatcccggc cacgctgatc aacgtcgggc
     1261 tggcgctccc gatcgcgttc cgcatgcgcc agaagtcgcg ctaccagcgc tgggtcacga
     1321 ccatcctggt ggtgccgatt acgctgggca cggtgctgat cgccgagggc atgctggcct
     1381 acctcggccc gaagggctgg ctgtcgcaga tgctgcagct cctgcacctg tacgacggac
     1441 cgatccgcct gacgcacaac tactggggcg tgctgctctc gctcgtcatc tccggcttcc
     1501 ctttcgcctt cctgctgatc ctttcctaca tcaccggcat cgacccggtg ctcgagcgcg
     1561 ccgcggcgac gctgggggcg gatgcgtggc gccgcttccg ccatatttat ttgccgctgc
     1621 tcgcgccggg tctcgccatc accttctgcc tcgccttcgt gcaggcattc tcggtgtttc
     1681 cgtcggcggt gctggtgggt gcgccggcgg gctcgacgcg cgtgatttcg ctcgccgcgt
     1741 acgaggcggc cttcgagcag tacgactatc cgatggcctc cgccgtggcc atgatcatgg
     1801 ggttcgcgca gctcgccgtg gttgccgcgg tgcttgccct caggcgcggc ttctaccgcg
     1861 ggccggtgac cgggggcaaa ggatgaaggc ctggctcgga cgcgcatggg gcgcgctgct
     1921 tctgggcagc atggcgctgt tcgtcgtcaa catcgggctg atggtggcgg cggtggccac
     1981 gaactccttc gccaagcgct ggctcggcac ctggctccct gagggctaca ccacgtcctg
     2041 gtacgccgcc gcgtggaagg agttccagct cggctgggtg ctgtgggtga cggtggaggt
     2101 ggtaggcgcg gtggtgctgc tgtcgatcct gatc
//
LOCUS       DASYJA010000234         2117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_125624, whole genome shotgun sequence.
ACCESSION   DASYJA010000234 DASYJA010000000
VERSION     DASYJA010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125624"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1399)
                     /locus_tag="VGO02_04955"
     CDS             complement(<1..1399)
                     /locus_tag="VGO02_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HEV7477071.1"
                     /translation="MNTKLITILSGVALWLAGIGLALAQANAIEGFEVAEQAGKVVVR
                     ITTKEALRSVPPNFAIASPARIAFDFPNTANGLGRASQDIGQGELRSMNVVQGAERTR
                     LVLNLRRSVVHEASLDGRALVVTLSAPAVAQTAPGGQVSHFAEGREDAKHAVRDIDFR
                     RGRAGEGRVVVDLTDAATGIDIRAQGQNIVVDFLKTTLPESLRRRLDVVDFATPVSTI
                     SAFQQGENVRMIIEPKGQWEHSAYQSDTQFVVEVKPVAADPSRVSQRGRYTGEKLSLN
                     FQNVEVRAVLNVIADFTDLNIITSDTVSGNLTLRLKDVPWDQALDIILQTRGLDSRRS
                     GNVIWIAPRDELATREKLALESQQQITDLEQTRTESFQMNYQKAADVQKLLSDASQRI
                     LSKRGSAVVDARTNTLFVQDTPGRLEDVRRLIAKIDVPVRQVMIEARIVEANDSFSKS
                     LGARIGYHDLRTSSGR"
     gene            complement(1396..1935)
                     /locus_tag="VGO02_04960"
     CDS             complement(1396..1935)
                     /locus_tag="VGO02_04960"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEV7477072.1"
                     /translation="MKTAAPYAAALAAALLLTACTGEEFGDLKAELNEKSKDFRGRVE
                     PLPQVKPYEPVPYTAEGVVDPFRPDRIDVAGAPRTPRADMSKMAPDLNRPKEPLEAFP
                     LESLQMLGTIMQNRETFGLVKAGPNLYRVKKGNYLGLNFGVVTGIDEVTINLKELVQD
                     GQGEWVERASALQLLEIRK"
     gene            complement(1932..>2117)
                     /gene="pilO"
                     /locus_tag="VGO02_04965"
     CDS             complement(1932..>2117)
                     /gene="pilO"
                     /locus_tag="VGO02_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEV7477073.1"
                     /translation="AFASDVGQLSRIVTLNDVSIIAGKDGVLTMDATARTFRYLDDEE
                     VSAQRKSQQAAKKGGRK"
BASE COUNT          291 a          714 c          706 g          406 t
ORIGIN      
        1 tccgcccgct gctggtgcgc agatcgtgat aaccgatgcg cgcgccgagg ctcttcgaga
       61 agctgtcgtt cgcttcgacg atgcgcgcct cgatcatcac ctggcgcacc ggcacgtcga
      121 tcttcgcgat caggcggcgc acgtcctcga gacggcccgg cgtgtcctgc acgaacagcg
      181 tgttggtgcg cgcatccacc acggcgctgc cgcgcttgga caggatgcgc tgcgaagcgt
      241 cggacagcag cttctgcacg tcggccgcct tctggtagtt catctggaac gactcggtgc
      301 gcgtctgctc caggtcggtg atctgctgct gcgactcgag cgccagcttc tcgcgcgtgg
      361 cgagctcgtc gcgcggcgcg atccagatga cgttgccgct gcgccggctg tcgaggcccc
      421 gcgtctgcag gatgatgtcg agcgcctggt cccagggaac gtccttcagc cgcagggtga
      481 ggttgcccga cacggtgtcg ctggtgatga tgttcaggtc ggtgaagtcg gcgatcacgt
      541 tgagcaccgc gcgcacttcc acgttctgga agttgagcga cagcttctcg ccggtgtagc
      601 ggccgcgctg cgagacgcgc gagggatcgg ccgcgaccgg tttcacctcg accacgaact
      661 gggtgtcgga ctggtaggcg ctgtgctccc actgcccctt cggctcgatg atcatgcgca
      721 cgttctcgcc ctgctggaag gcgctgatcg tgctgaccgg cgtggcgaag tcgaccacgt
      781 cgaggcgccg ccgcaggctc tcgggcagcg tggtcttgag gaagtcgacg acgatgttct
      841 ggccttgcgc gcggatgtcg atgccggtgg cggcgtcggt gagatcgacc actacgcggc
      901 cttcgcccgc ccggccgcgc cggaaatcga tgtcgcgcac cgcgtgcttg gcgtcctccc
      961 gtccttcggc gaagtgcgat acctggccgc cgggcgcggt ctgggcgaca gccggcgccg
     1021 acagcgtgac caccagcgcg cgaccgtcga ggctggcctc gtgcaccacg gagcggcgca
     1081 gattgagcac cagccgcgtg cgctcggcgc cctgcaccac gttcatgctg cgcagctcgc
     1141 cctgcccgat gtcctggctg gcgcggccga ggccattcgc cgtgttgggg aaatcgaacg
     1201 cgatgcgcgc gggtgaagcg atggcgaagt tgggcggcac gctgcgcagc gcctccttgg
     1261 tggtgattcg caccaccacc ttgcctgcct gctcggctac ctcgaagccc tcgatcgcgt
     1321 ttgcctgcgc aagcgccagc ccgattccgg ccagccacag cgcgacgccg ctaaggattg
     1381 tgatgagttt tgtgttcatt ttcttatctc caacagttgc agcgcgcttg cacgctccac
     1441 ccattccccc tgcccgtcct gcaccagctc cttgaggttg atcgtgacct cgtcgattcc
     1501 cgtgacgacg ccgaagttca gccccaggta attgcccttc ttgacgcggt acaggttcgg
     1561 tccggccttc accagcccga aggtctcccg gttctgcatg atcgtgccga gcatttgcag
     1621 cgactccagc gggaacgcct cgagcggctc tttcggccgg ttgaggtcgg gcgccatctt
     1681 gctcatgtcg gcgcgcggcg tgcgcggcgc gccggcgacg tcgatgcggt ccgggcggaa
     1741 cggatcgacc acgccctcgg cggtgtacgg caccggctcg tagggcttca cctgcggcag
     1801 cggctcgacg cggccgcgaa agtccttgct cttctcgttg agctcggcct tcaggtcgcc
     1861 gaattcctca ccggtgcacg ctgtaagcag gagggccgcg gcgagcgcgg ccgcatatgg
     1921 cgcggcggtt ttcatttcct gccgcctttc ttcgcagcct gctggctctt gcgctgggcc
     1981 gaaacttcct cgtcgtcgag gtaacggaag gtgcgcgcgg tagcgtccat cgtcagcacg
     2041 ccgtccttgc cggcgatgat cgaaacgtcg ttcagcgtca cgatgcgcga cagctggccg
     2101 acgtcgctcg cgaaggc
//
LOCUS       DASYJA010000235         5283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2214894, whole genome shotgun sequence.
ACCESSION   DASYJA010000235 DASYJA010000000
VERSION     DASYJA010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2214894"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VGO02_04970"
     CDS             complement(<1..600)
                     /locus_tag="VGO02_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477074.1"
                     /translation="MNLRTLAAAALAAPVLAFAQAAPQPADKPAAETPAAEKPAGEKP
                     AAAKPAAKAGKAPAKPAAGAVATVNGIAVPRSRLDLMMRQQAARGGQDNDQMRMLMRE
                     ELINRELIAQEAGRAGVAKSADVQAQLDLVRQEIVVGAYIRDWVGKHPITDADVQKEY
                     DRAKTQAGDKEYRARHILLETEDQAKGMIADLKKGAKFDE"
     gene            complement(597..875)
                     /locus_tag="VGO02_04975"
     CDS             complement(597..875)
                     /locus_tag="VGO02_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEV7477075.1"
                     /translation="MSVAQRIRERLAALEPLALDLVDESAQHAGHAGERPGGGTHWRL
                     AIVSPRFAGKPTVARHRMVYEALGELMQDPIHALAISARSPEETKGKQ"
     gene            complement(872..1405)
                     /locus_tag="VGO02_04980"
     CDS             complement(872..1405)
                     /locus_tag="VGO02_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEV7477076.1"
                     /translation="MKFLFDLLPVGLFFVAIFLWDIFVATAVAIAATVAQVAWLKLRK
                     RKVEPMLWASLGIILVFGGLTLYLKDKTFILWKPTVLYWLFALVLTVAAFMRRNLIRS
                     LLSEQMRLPDAIWTRLNWSWVAFFAFMGGANLYVAFNYSEKVWASFKLFGGMGLMVLF
                     VVVQSLLLARHVDEEKP"
     gene            complement(1437..2120)
                     /locus_tag="VGO02_04985"
     CDS             complement(1437..2120)
                     /locus_tag="VGO02_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477077.1"
                     /translation="MIDFLAQLQPQYLLVGFTVLGGLLALPLVFFAIRRLTDFRFVSG
                     SVLMLCSALVVALGVAAALAAASLRTYHRLTHEQEAARVAMRQIAPKHYALSVQPTGA
                     RLQSYEILGDEWQIDARLLKWRPIATIAGFDTVYRLERLSGRYSDLAEERTAKRTVHG
                     LAERVPVDFWVFLRRYNDYLPFGDALYGSAAFVPMADGAEYLVTVSSTGLVVRPANDA
                     ARKAVGAWK"
     gene            complement(2117..2563)
                     /locus_tag="VGO02_04990"
     CDS             complement(2117..2563)
                     /locus_tag="VGO02_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEV7477078.1"
                     /translation="MLLKGSCHCRSVRFSLRSETPYPYMWCYCSICRKTAGGGGYAIN
                     IMGRADSLQVKGRTKPYRATRGGGERHFCPKCASALWVWDKRWAEWVYPFASAIDTKL
                     PVPRERQLIFLESKANWAQVPDGKRFGEYPKESIIDWHRKRGLLAA"
     gene            2599..2991
                     /locus_tag="VGO02_04995"
     CDS             2599..2991
                     /locus_tag="VGO02_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV7477079.1"
                     /translation="MSLVTLIEYADAPQEVRSVYDDIMKTRGTDWINNFWKALANNPA
                     ELKRVWANVKQVMAPGALDPLVKEMVYLAVSVTNGCEYCTYSHHAAARKKGMSEAMFM
                     ELMAVVGLANETNRLADGLRIDVDRQFK"
     gene            2969..4147
                     /locus_tag="VGO02_05000"
     CDS             2969..4147
                     /locus_tag="VGO02_05000"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7477080.1"
                     /translation="MSTGSSSNRGFALYLGVVQFFFATTWTIYVLYLPQLAAQAGIDE
                     RWVPWILVADQAVFAVMDVVTGFWIDRVRAGLARFGGWILGITVVSSLAFVFLPFVGS
                     TLMLLCLAVWAVTSSALRSPPWALLGRYAATPALPWLSALVLTGNALAAAAAPYLGIA
                     LRGVSPTLPFLLSTLTLVATVAGLVYIERRSVRSETVGEISKEDPWRIGLFFAALLLL
                     AVGFQVHFSLNSAPQYLRFAAATDLPWLMPVFWIGFNVLMFPAAGLVERLGASRTMAL
                     AGAVGALAALAAALAASLPALVAAHFVAGGAWGAASVAAYSAAIAFGRTGREGRFLGT
                     LFAMLAIAAFARIAAVASGFALDPAVRTLLPWLPQTAWLLAALLLVFALRPARAFRQA
                     "
     gene            4579..>5283
                     /locus_tag="VGO02_05005"
     CDS             4579..>5283
                     /locus_tag="VGO02_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HEV7477081.1"
                     /translation="MAKRRVVLKALSMAAAIAAIGFGSPALYAQQKTVKIGVLHSLSG
                     TMAISETVLKDTVLMAVEEINAKGGVMGMKIEPVVVDPASNWPLFAEKARQLISQDKV
                     AVTFGCWTSVSRKSVLPVYEELNSLLFYPVQYEGEEISRNVFYTGAAPNQQAIPAVEY
                     LMSKDGGSAKRWVLLGTDYVYPRTTNKILRAFLKSKGVDDADIMEDYTPFGHADYQTI
                     VADIKKFAQGAPTAVVS"
BASE COUNT          819 a         1736 c         1794 g          934 t
ORIGIN      
        1 ctcgtcgaac ttggcgccct tcttcaggtc cgcgatcatg cctttcgcct ggtcctcggt
       61 ctcgagcagg atgtggcgcg cgcgatactc cttgtcgccc gcctgcgtct tggcgcggtc
      121 gtactccttc tgcacgtcgg cgtcggtgat cgggtgcttg cccacccagt cgcggatgta
      181 ggcgccgacc acgatctcct ggcgcaccag gtcgagctgc gcctgcacgt ccgcgctctt
      241 ggcgaccccg gcgcggccgg cttcctgcgc gatcagctcg cggttgatca gctcctcgcg
      301 catcagcatg cgcatctggt cgttgtcctg gccgccgcgc gcggcctgct ggcgcatcat
      361 gaggtccagg cgcgagcgcg gcacggcgat gccgttgacg gtggcgacgg ccccggctgc
      421 cggcttggcg ggcgccttgc cagccttggc ggcgggtttg gcggccgcgg gcttttcgcc
      481 cgccggcttt tcggccgccg gcgtttcggc cgcgggcttg tcggcgggct gcggtgcggc
      541 ctgcgcgaag gctagaacgg gcgcggcgag cgcggcggcg gcgagtgtgc ggagattcat
      601 tgctttcctt tcgtttcttc tgggctgcgc gcgctgatgg cgagcgcatg gatggggtcc
      661 tgcatcagct cgccgagcgc ctcgtacacc atgcggtggc gcgcgacggt gggcttgccg
      721 gcgaagcgcg gcgagacgat ggcgagccgc cagtgcgtgc cgccgccagg gcgctcgccg
      781 gcgtggccgg cgtgctgcgc gctctcgtcg accaggtcga gcgcgagcgg ctcgagcgcg
      841 gcgaggcgct cgcggatgcg ttgcgcgacg ctcaaggctt ctcctcgtcg acgtgcctgg
      901 cgaggagcag cgactgcacc acgacgaaca ggaccatgag ccccatgccg ccgaacagct
      961 tgaacgaggc ccataccttt tcggaatagt tgaacgcgac gtagaggttg gcgccgccca
     1021 tgaaggcgaa gaaggcgacc cagctccagt tgaggcgcgt ccagatcgcg tcgggcaagc
     1081 gcatctgctc cgacagcagg ctgcggatca ggttgcggcg catgaaggcg gccaccgtca
     1141 gcaccagcgc gaacagccag tagaggaccg tcggcttcca caggatgaac gtcttgtcct
     1201 tcaggtacag cgtcaggccg ccgaacacca ggatgatgcc gaggctcgcc cagagcatcg
     1261 gctcgacctt ccgcttgcgc agcttgagcc aggcgacctg cgcgacggtc gcggcaatgg
     1321 cgaccgccgt ggcgacaaag atgtcccaga ggaagatcgc tacgaagaaa agacccaccg
     1381 gaagcaggtc gaacaggaac ttcatgtgca accacggatt ctaccgggct tttcccctac
     1441 ttccaggcgc ctaccgcctt gcgcgcggcg tcgtttgccg ggcgcacgac caggccggtg
     1501 ctcgaaaccg tcaccaggta ctcggcgccg tcggccatcg gcacgaaggc ggcgctgccg
     1561 tagagcgcgt cgccgaacgg caggtagtcg ttgtagcgcc tcaggaacac ccagaagtcg
     1621 accggcaccc gctcggcgag gccgtgcacg gtgcgcttgg ccgtgcgctc ctcggcgaga
     1681 tccgaatagc ggcccgacag cctttccagc cggtagaccg tgtcgaagcc ggcgatggtg
     1741 gcgatgggcc gccacttcag caggcgcgcg tcgatctgcc actcgtcgcc gaggatctcg
     1801 tagctctgca ggcgcgcgcc ggttggctgg acgctgagcg catagtgctt cggcgcgatc
     1861 tggcgcatcg cgacgcgagc cgcctcctgc tcgtgggtga ggcggtggta agtgcgcagg
     1921 ctcgccgcgg cgagcgccgc ggccacgccc agcgccacga ccagcgcgga gcagagcatg
     1981 aggaccgagc cgctcacgaa gcgaaagtcc gtcagccggc gtatcgcgaa gaagacgagc
     2041 ggcagcgcca gcaggccgcc gagcacggtg aatcccacca ggaggtactg cggttggagc
     2101 tgcgccagga agtcgatcat gccgcgagca gcccgcgctt gcgatgccag tcgatgatgg
     2161 attccttcgg gtattcgccg aagcgcttgc cgtccggcac ctgcgcccag ttggccttcg
     2221 actcgagaaa aatgagctgg cgctcgcgcg gcacggggag cttcgtgtcg atcgcggaag
     2281 cgaacggata gacccactcc gcccagcgct tgtcccacac ccacagagcg ctggcgcatt
     2341 tggggcagaa gtggcgctcg ccgccgccgc gtgttgcccg gtatggcttc gtcctgccct
     2401 tcacctggag ggaatcggcg cggcccatga tgttgatggc atagccgccg ccgccggcgg
     2461 tcttgcgaca gatgctgcag tagcaccaca tgtaggggta gggcgtctcc gatcgcagcg
     2521 agaagcgcac cgagcggcag tggcaggagc ctttcagtag cattcgatta ataataccgt
     2581 gcaaccaagg aggcctcctt gtcattagtc acacttatcg agtacgccga cgccccccag
     2641 gaggtgcgtt cggtctatga cgacatcatg aaaacgcgcg gcaccgactg gatcaacaac
     2701 ttctggaagg cgctcgccaa caaccccgcc gagctcaagc gcgtctgggc caacgtaaag
     2761 caggtgatgg cgcccggcgc gctcgatccg ctggtgaagg aaatggtcta cctcgctgtc
     2821 agcgttacca acggctgcga gtactgcacc tactcccacc atgccgccgc gcgcaagaag
     2881 ggcatgagcg aggcgatgtt catggagctg atggcggtgg tcggcttggc gaacgagacc
     2941 aaccgtcttg ccgacggctt gcgcatcgat gtcgaccggc agttcaagta acagggggtt
     3001 cgcgctctac ctcggcgtcg tccagttctt cttcgcgacg acgtggacga tctacgtcct
     3061 ctacctgccg cagctcgccg cgcaggccgg catcgacgag cgctgggtgc cgtggatcct
     3121 ggtcgccgac caggcggtgt tcgccgtcat ggacgtcgtc accggattct ggatcgaccg
     3181 cgtgcgcgcc ggcctcgccc gcttcggcgg atggattctc ggcatcacgg tggtgtcttc
     3241 cctggcattc gtatttctgc cgttcgtcgg ctccacgctg atgttgctat gccttgcggt
     3301 ctgggccgtg acctcatcgg cattgcgctc gccgccgtgg gcattgctcg gccgctacgc
     3361 ggcgacgccg gcgctgccgt ggctctcggc gctggtgctg accggaaatg cgctcgccgc
     3421 ggccgccgcg ccttaccttg gtatcgctct gcgcggtgtc agcccgacgc tgccttttct
     3481 tctttcgacc ttgaccctcg tcgctaccgt cgctggactc gtctacatcg aacgccgcag
     3541 cgtgcggagt gaaaccgtag gggaaatatc gaaggaagac ccgtggcgga tcgggttgtt
     3601 cttcgccgcg ctgctcctcc tggcggtcgg cttccaggtc catttctcgc tcaactcggc
     3661 gccgcaatac ctgcgcttcg cggcggcgac cgatctgccg tggctgatgc cggtgttctg
     3721 gatcggcttc aacgtgctga tgttccccgc ggccggcctg gtcgagcgcc tcggcgcttc
     3781 gcgcacgatg gcgctggcgg gtgcggtcgg ggcgctcgcc gcgcttgccg cggcgctggc
     3841 cgccagcctg ccggcgctgg tggcggcgca tttcgtggct ggcggcgcgt ggggcgcggc
     3901 gagcgtagcc gcctactcgg cggcgatcgc gttcggccgc accggccggg aagggcgctt
     3961 cctcggcacg ctgttcgcga tgctcgccat cgcggctttc gcgcgcatcg ccgcggtggc
     4021 gagcggcttc gcgctcgatc ccgccgtcag gacgctcctg ccgtggctgc cgcagaccgc
     4081 ctggctcctc gcggcgctgc tgctggtctt cgctcttcgt ccagcacgcg ctttccgtca
     4141 ggcgtgacgt agaagcgccg ccacaccttg ccctgcgcgc tgacctcgac cgtccaatag
     4201 cagcggccgg tgtgctcgat ctgctgctcc aaaagcaggg cgtattgcgc gactgcgccg
     4261 ctcttgtgga tgtaatccgc ccaggcttcg gtctcgggta gcagcttcac cttggtcacc
     4321 gcgcggcgca gcgggaggtt gcccaggtcg gggtattcgc aggccagagc cggagtcccg
     4381 caccaaagca gtgcgagcag tccgattcgg tgcatgagtc gccgcatcga ttctccgagg
     4441 gaagggaaaa agccttacgc cgcacgagca tagctcgtct tcgggcgcgt ggcatacctc
     4501 ttgcgcctag aggttcggga ggtcgccggt caccggggac tggtcatcaa ttcgaatctc
     4561 tagggaagga aattggcaat ggcaaaacga cgcgtagtac tcaaggcgct gtcgatggcg
     4621 gctgcaatcg cggccatcgg gttcggctcg ccggccctgt acgcgcagca aaagacggtg
     4681 aagatcggcg tgctgcattc gctgtcgggc acgatggcga tcagcgagac ggtgctgaag
     4741 gacacggtgc tgatggccgt ggaagagatc aacgccaagg gcggcgtgat gggcatgaag
     4801 atcgagccgg tggtggtcga tccggcttcg aactggccgc tgttcgccga gaaggcgcgc
     4861 cagctgatct cgcaggacaa ggtagccgtc accttcggct gctggacctc ggtttcgcgc
     4921 aagtccgtgc tgccggttta cgaagagctg aacagcctgc tcttctaccc ggtgcagtac
     4981 gagggcgaag agatctcgcg caacgtgttc tacaccggcg ccgcgcccaa ccagcaggcc
     5041 atcccggcgg tcgagtacct gatgtccaag gacggcggct cggccaagcg ctgggtgctg
     5101 ctcggcacgg actacgtgta cccgcgcacc accaacaaga tcctgcgcgc cttcctcaag
     5161 tcgaagggcg tcgacgacgc cgacatcatg gaggactaca cgccgttcgg gcacgcggac
     5221 taccagacca tcgtcgcgga catcaagaag ttcgcacagg gtgcgccgac cgcggtggtg
     5281 tcc
//
LOCUS       DASYJA010000236         2435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1488769, whole genome shotgun sequence.
ACCESSION   DASYJA010000236 DASYJA010000000
VERSION     DASYJA010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488769"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VGO02_05010"
     CDS             complement(<1..713)
                     /locus_tag="VGO02_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEV7477082.1"
                     /translation="MAAVTLPDEHALILAGAGSGKTRVLTTRIAWLIKGARASPANFL
                     AVTFTNKAAREMLTRLTAMLPVSPRGMWMGTFHGLCNRMLRAHHRDAGLPQAFQILDS
                     QDQLAAIKRLLKGMNVDEDRFPPREVQWFINGQKEEGVRARDVALTDDTVRRQAQLYA
                     AYDEQCQREGVVDFPELLLRSYELLRRNEILREHYAQRFRYVLVDEFQDTNRLQYRWL
                     KLFSSSTNVFFCVGDDDQS"
     gene            complement(821..1687)
                     /locus_tag="VGO02_05015"
     CDS             complement(821..1687)
                     /locus_tag="VGO02_05015"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEV7477083.1"
                     /translation="MKLLSRYALLAAALIAAAPALAQPAAVVEGVQMPAWVERSGRTS
                     PVAAGMALQAGDQVRTGAGSRLLVKLSEGSLVKLGENGTLRIAEMVPSPDLFKAALRV
                     FEGAFRFTTDLAAKQRRRDVNITVATVTAGIRGTDLWGRSRDERQIVCLIEGKIEVGA
                     EGEQAVTMDQPRQFYIRDKGKTQPVGFVEPQQLNEWAKETEIERGKGAARRGGKWSVT
                     LATADSQRSALAVYDAVRKAGYPAQIESTKEGDKRSYQVRIRNLPSKAEAAALADSLR
                     GKHGVEKPIVSG"
     gene            1805..>2435
                     /locus_tag="VGO02_05020"
     CDS             1805..>2435
                     /locus_tag="VGO02_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEV7477084.1"
                     /translation="MQDTYDPTAVEREAQQYWGANASFRSTEDPARPKFYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLTRYYRMRGYNVLQPMGWDAFGLPAENAAMANKVPPAKWTR
                     DNIAYMKKQLQGLGFALDWERELATCDASYYKWNQWLFTRLFRKGLAYKKTGVVNWDP
                     VDQTVLANEQVIEGRGWRTGAVVEKREIPMYFFRITAYAEELLAALDHLL"
BASE COUNT          380 a          821 c          805 g          429 t
ORIGIN      
        1 atgctctggt cgtcgtctcc gacgcaaaaa aatacgtttg tcgaagaaga aaaaagcttt
       61 agccaccgat actgcaaccg gttggtgtcc tggaactcgt cgaccaggac atagcggaag
      121 cgctgcgcgt agtgctcccg caggatttcg tttcttctca gcagctcgta ggagcgcagc
      181 agcagttcgg gaaagtccac cacgccctcg cgctggcact gctcgtcgta ggccgcgtac
      241 agctgcgcct ggcggcgcac tgtatcgtcg gtcagggcga cgtcgcgcgc gcgcacgccc
      301 tcctccttct ggccgttgat gaaccactgc acctcgcgcg gcgggaagcg gtcctcgtcg
      361 acgttcatgc ctttgagcag gcgcttgatc gcggcgagct ggtcctgcga gtcgaggatc
      421 tggaacgcct gcggcagccc ggcgtcgcgg tgatgcgcgc gcagcatgcg gttgcacagg
      481 ccgtggaagg tccccatcca catgccgcgc gggctcaccg gcagcatcgc ggtgaggcgc
      541 gtcagcatct cgcgcgccgc cttgttggtg aaggtgaccg ccaggaagtt cgcaggcgag
      601 gcgcgagcgc ccttgatcag ccaggcgatg cgcgtggtga gcacccgcgt cttgccgctg
      661 cccgcgccgg ccaggatcag cgcgtgctcg tcgggaagcg tgactgcggc cagctgctcc
      721 gggttgaggt gttcgaggtg aagcgaccag cagcgatttt atcgcggctg cgcgtgactt
      781 ctgcgcacaa gctcgcggat tttatcgcgg ccgcgccctg tcagcccgaa acgatcggct
      841 tttctacgcc gtgcttgccc cgcaggctgt ccgcgagcgc ggccgcctcg gctttcgacg
      901 gcaggttgcg gatgcgcacc tggtagctgc gcttgtcgcc ttccttggtc gattcgatct
      961 gcgccgggta gcccgccttg cgcaccgcgt cgtacacggc aagcgcggag cgctggctgt
     1021 cggcggtcgc gagcgtcacg ctccacttgc cgccgcggcg cgccgcgccc ttgccgcgct
     1081 cgatctcggt ttccttcgcc cattcgttga gctgctgcgg ctcgacaaag ccgaccggct
     1141 gggtcttgcc cttgtcgcgg atgtagaact ggcgcggctg gtccatggtc acggcctgct
     1201 cgccctcggc gccgacctcg atcttgccct cgatcaggca gacgatctgg cgctcgtcgc
     1261 gcgagcgccc ccacagatcc gtgccgcgga tgccggcggt caccgtggcg accgtgatgt
     1321 tgacgtcgcg ccggcgctgc ttggcggcga ggtcggtggt gaagcgaaac gcgccttcga
     1381 acacgcgcag cgccgccttg aacagatccg gcgaaggcac catctcggcg atgcgcaggg
     1441 tgccgttctc gcccagcttg accaggctgc cttcggaaag cttcaccagc aggcgcgagc
     1501 cggcgccggt gcgcacctgg tcgccggcct gcagcgccat gccggccgcg accggcgaag
     1561 tgcggcccga gcgctccacc caggccggca tctgcacgcc ctcgacgacg gccgcgggct
     1621 gggcgagcgc cggcgcggcc gcgatgagcg ctgcggcgag gagcgcatag cgcgaaagca
     1681 gtttcatttt ctttcccctt cttgcgtcat cttaccctcc cgttgatgct gcttatacgg
     1741 ctgcagtccc taaggcggat gcaggcgaaa gctataatcc gcggcttccc gagcatttgg
     1801 tcacatgcag gatacctacg accccaccgc agtcgagcgc gaggcccagc agtactgggg
     1861 cgcgaacgcg tctttccgct cgaccgagga tcccgcgcgg cccaagttct actgcctgtc
     1921 catgttcccc tatccgtcgg ggaagctgca catggggcat gtgcgcaact acacgatcgg
     1981 cgacgtcctc acgcgctact accgcatgcg cggctacaac gtgctgcagc ctatgggctg
     2041 ggacgccttc ggcctgccgg ccgagaacgc ggcgatggcg aacaaggtgc cgccggcgaa
     2101 gtggacgcgc gacaacatcg cgtacatgaa aaagcagctg cagggtctcg gcttcgccct
     2161 cgactgggag cgcgagctcg ccacctgcga cgcgagctac tacaagtgga accagtggct
     2221 gttcacgcgc ctgttcagga aggggctcgc ctacaagaag accggggtgg tgaactggga
     2281 cccggtcgac cagaccgtgc tcgccaacga gcaggtgatc gagggccgcg gctggcgcac
     2341 cggcgcggta gtcgagaagc gcgagatccc gatgtacttc tttcgcatca cggcctacgc
     2401 cgaggagctc ctagcggccc tcgatcacct gctcg
//
LOCUS       DASYJA010000237         5779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_399203, whole genome shotgun sequence.
ACCESSION   DASYJA010000237 DASYJA010000000
VERSION     DASYJA010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399203"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1039
                     /gene="serC"
                     /locus_tag="VGO02_05025"
     CDS             2..1039
                     /gene="serC"
                     /locus_tag="VGO02_05025"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEV7477085.1"
                     /translation="MLPAEVLSRAGDEMLDWRGSGMGVMEMSHRGKEFISIYEETERD
                     LRELMGVPANYKILLLQGGATLQFAQIPMNLLRGKAKADYINTGEWSKKAIKEAKAYC
                     DVHIAASSEDKNFTYAPKSWDVRKDAAYVHYCSNETIGGVEYHQIPKAEVPLVADASS
                     HFLSRPVDVSKFGLIYAGAQKNAGPAGLTFVIVRDDLIGQVAKGTPSVMDYKLQAEAD
                     SMLNTPASYSIYVAGLVFKWLKQLGGLPEIERRNIAKAKLLYDYLDSSKFFRNPVPKE
                     DRSRMNVPFTLADANLDAPFLQGAERQGMVQLKGHRSVGGMRASIYNAMPIEGVQALV
                     SYMKDFEKKHV"
     gene            1032..2216
                     /locus_tag="VGO02_05030"
     CDS             1032..2216
                     /locus_tag="VGO02_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEV7477086.1"
                     /translation="MSKKKCEILVLNNVAQVGLKRFPAERYAVVKESKDPWAILVRSA
                     DLHKHEFGPALRAVGRAGAGVNNIPVAALSKRGIPVFNAPGANANAVKELVLAGMLIA
                     ARNLAPALEFVSKQEESPDFEKKIEDGKKQFAGFELPGHTLGVIGLGKIGSLVADAAI
                     RLGMNVLGYDPHITVEAAWSLPSQVKRASSVEEVLKASHFVTLHIPLMDKTRNTISAK
                     NVELMRAGAVLLNFSREGIVAEEAVQKGLASGKLKWYVTDFPSPGLLAAKGVIALPHL
                     GASTGEAEDNSAVMVVDQLREYLEHGNLQNAVNFPDAAIPREAPYRLAIANANVPDML
                     GRISHALGKRKINIHNMLNKSKGDMAYTLVDTDAPVVAEALKELEDLTGVLAVRYLPV
                     SE"
     gene            2209..3279
                     /gene="pheA"
                     /locus_tag="VGO02_05035"
     CDS             2209..3279
                     /gene="pheA"
                     /locus_tag="VGO02_05035"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV7477087.1"
                     /translation="MSSELDKLRKEIDRIDDELLGALERRAGVVRKVGELKGAAPAYR
                     PEREAQILRRIVSQSKNLPGDGAAAIFREIISACRGLEEAIRVAYLGPEGTFSEQAVR
                     KHFGAAVEASPKASVDDAFRSAESGATQFTVVPVENSTDGVVGRTLDLLTSTPLRICG
                     EVELRVQQNLLSKEKALKGIKRVYSHSQSLAQCNGWLDKNLPKAARIPVASNAEAAQR
                     AAKEKGAAAIAGLLAGSRYKLNVLARSIEDSPDNTTRFLVLGQIDPAPTGKDRTSLVM
                     SAENRPGAVHGLLTPLAEHKVSMTRIESRPLRTRASLWEYFFFIDVEGHQRDPAVARA
                     IDGLREKAPFLKILGSYPVAVS"
     gene            3281..4402
                     /gene="hisC"
                     /locus_tag="VGO02_05040"
     CDS             3281..4402
                     /gene="hisC"
                     /locus_tag="VGO02_05040"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEV7477088.1"
                     /translation="MKIDLCELSPSYVRSIAPYQPGKPIAELAREMGLDEAGIVKLAS
                     NENPRGIGPRTRAAIEASIADIARYPDGNGFELKQALAKRYGVDMASIVLGNGSNDAL
                     ELVALAFLGLGRAAVYSQHCFAVYPLATQARGARSIVVPAKDFGHDLEAMAKAIDDET
                     YVAWIANPNNPTGTYAKGEAVEAFLRRVPERVLVVLDEAYNEYLPHELKADSARWLKR
                     HPNLVITRTFSKAYGLAGLRVGYALAHPSVADVMNRVRQPFNVNSMALAAATAALDDM
                     EFVARSYAENLQGLRQIEEGARRLGLDFIPSYGNFITVRVAQGGKSAAEIYKRLLRRS
                     VIVRPVGGGYGLPEHLRVTVGTAPENEKFLGALAASLKD"
     gene            4402..5292
                     /locus_tag="VGO02_05045"
     CDS             4402..5292
                     /locus_tag="VGO02_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEV7477089.1"
                     /translation="MLFQRVAVVGVGLIGGSFALALKQAAACGHVVGVGRNPVNLRLA
                     LERGIIDSMESDAARAAEGADLVLLAAPVGQFPKIFGRVGAALKPGAILTDAGSTKRD
                     VVAAARKALKKKIAQFVPAHPIAGAEKSGAGAANAELFRGRRVVITPLKENPAQAVEK
                     VNQAWSTCGARTSQMTPEQHDAVFAAVSHLPHLLAYVLVHDVAGRKNANELFSFAAGG
                     FRDFTRIASSHPEMWRDICVANADRIGAELASYTKKLAGVKKLLARRDARALEKLFDE
                     ARDARESWLQKTWAGRKPGS"
     gene            5292..>5779
                     /locus_tag="VGO02_05050"
     CDS             5292..>5779
                     /locus_tag="VGO02_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEV7477090.1"
                     /translation="METLRLEPVRRAAGAVRLPGSKSISNRVLLLAALAKGETEIGGL
                     LDAEDTKVMREALSRLGIVFKGQTIVGAGGAFPVRKADLFLGNAGTAFRPLTAALAFC
                     NGEYRLSGVPRMHERPIGDLVDALRGIGARIDYLRTDGFPPLALHPSRLKTENVKVRG
                     DVS"
BASE COUNT         1057 a         1911 c         1931 g          880 t
ORIGIN      
        1 gatgctcccc gccgaggtgc tatcccgcgc cggcgacgag atgctcgact ggcgcggcag
       61 cggcatgggc gtgatggaga tgagccaccg cggcaaggag ttcatctcga tctacgagga
      121 aaccgagcgc gacctgcgcg aattgatggg cgtgccggcg aactacaaga tcctgctcct
      181 gcagggcggc gccacgctgc agttcgcgca gattcccatg aacctgctgc gcggcaaggc
      241 caaggccgac tacatcaaca ccggcgagtg gtcgaagaag gcgatcaagg aagcgaaggc
      301 ctactgcgac gttcacatcg ccgcctcctc cgaagacaag aacttcacct atgccccgaa
      361 gagctgggac gtgcgcaaag acgccgcgta cgtccactat tgctcgaacg agaccatcgg
      421 cggggttgaa tatcatcaaa taccaaaggc cgaagtgccc ctggtcgcgg atgcttcatc
      481 ccactttctt tcccgtccgg tagacgtatc gaaattcgga ctgatctacg cgggagcgca
      541 gaagaatgcg gggccggccg gcctcacctt cgtcatcgtg cgcgacgatt tgatcggcca
      601 ggtggccaaa ggcacgcctt cggtgatgga ctacaagctg caggccgagg ccgattcgat
      661 gctcaacacg ccggcgagct attcgatcta cgtcgccggg ctggtgttca agtggctgaa
      721 gcagctcggc ggcctgcccg agatcgaaag aaggaacatc gcgaaggcga agctgctgta
      781 cgactatctc gactcgagca agttcttccg caaccctgtt cccaaagaag accgctctcg
      841 catgaacgtg ccgttcacgc tcgcagatgc caatttggat gccccatttc tgcaaggcgc
      901 cgagcggcag ggcatggtgc agctgaaagg ccatcgctcc gtgggcggca tgcgcgcctc
      961 gatctacaac gcgatgccga tcgagggcgt gcaggcgctg gtctcctata tgaaggattt
     1021 cgagaagaaa catgtctaag aagaagtgcg agatcctggt gctgaacaat gtggcccagg
     1081 tcggcctgaa gcgctttccg gccgagcgct acgcggtggt gaaggaatcg aaggatccgt
     1141 gggcgatcct ggtgcgctcg gcggacctgc acaagcacga gttcgggcct gcgctgcgcg
     1201 cggtgggccg cgccggcgca ggcgtcaaca acattcccgt cgccgcgctc tccaaacggg
     1261 gcatcccggt tttcaacgcg cccggcgcga acgccaacgc ggtgaaggag ctggtgctcg
     1321 ccggcatgct gatcgcggcg cgcaatctcg cccctgcatt ggaattcgtc tccaagcagg
     1381 aagaatcgcc tgatttcgag aagaaaatcg aagacggaaa aaagcagttc gccggcttcg
     1441 agctgcccgg ccacacgctg ggagtgatcg gcctgggcaa gatcggcagc ctggtggccg
     1501 acgcggcgat ccgcctcggc atgaacgtgc tcggctacga cccgcacatc acggtcgagg
     1561 ccgcctggag cctgccctcg caggtgaagc gggcgtcttc ggtcgaggaa gtcctcaagg
     1621 cgagccactt cgtcacgctg cacatcccgc tgatggacaa gacgcgcaac acgatcagcg
     1681 cgaagaacgt cgagctgatg cgcgccggcg cggtgctgct caacttctcg cgcgagggca
     1741 tcgtcgccga ggaggcggtg cagaaggggc tcgcgagcgg caagctgaag tggtatgtca
     1801 ccgacttccc cagccccggc ctgctggcag ccaaaggcgt catcgccctg ccgcacctcg
     1861 gcgcctcgac cggcgaagcc gaggacaatt ccgcggtgat ggtggtcgac cagctgcgcg
     1921 aatacctcga gcacggcaat ctgcagaacg cggtcaattt ccccgacgcc gcgataccgc
     1981 gcgaggcgcc ctaccgcctg gcgatcgcca acgccaacgt gcccgacatg ctcgggcgca
     2041 tctcgcacgc cctgggcaag cgcaagatca acatccacaa catgctgaac aagtccaagg
     2101 gggacatggc ctacaccctg gtcgacaccg atgcgccggt cgtggccgag gcgctcaagg
     2161 agctggagga cctgaccgga gtgctcgcag tacggtatct tccggttagt gagtagcgaa
     2221 ctagacaagc tgcgcaagga aatagaccgc atcgacgacg agctgctggg cgcgctcgag
     2281 cgccgcgccg gcgtggtgcg gaaggtcggc gagcttaagg gcgccgcgcc cgcctaccgt
     2341 cccgagcggg aggcccagat ccttcgcagg attgtttctc aaagtaaaaa tcttccaggc
     2401 gacggggccg ccgcgatctt ccgcgagatc atctccgcct gccgcggcct ggaggaggcg
     2461 atccgcgtcg cctacctcgg acccgagggc acgttcagcg agcaggcggt gcgcaagcat
     2521 ttcggagcgg cggtcgaggc gagccccaag gcctcggtgg acgacgcgtt ccgcagcgcc
     2581 gaatcgggcg cgacgcagtt caccgtggtg ccggtggaga attccaccga cggcgtggtc
     2641 ggccgcacgc tcgacctgct gacctcgacg ccgctgcgca tctgcgggga agtggaattg
     2701 agggttcagc aaaatcttct ttcgaaggaa aaggcattga agggaataaa gcgtgtgtat
     2761 tcgcactcgc aatccctggc gcagtgcaac ggctggctcg acaagaacct gcccaaggcg
     2821 gcgcgcatcc cggtcgcctc caacgccgag gccgcgcagc gcgccgcgaa ggagaagggc
     2881 gccgcggcga tcgcgggcct gctggccggc agccgctaca agctgaacgt gctggcgcgc
     2941 tcgatcgagg actcgccgga caacaccacg cgcttcctcg tgctggggca gatcgacccg
     3001 gcgccgaccg gcaaggaccg cacctcgctc gtcatgtcgg ccgagaaccg gcccggcgcg
     3061 gtgcacgggc tgctcacgcc gctcgccgag cacaaggtga gcatgacgcg tatcgagtcg
     3121 cgcccgctgc gcacgcgcgc ctcgctgtgg gaatacttct tcttcatcga cgtcgagggc
     3181 caccagcggg accccgcagt ggcaagagcg atcgacggtc tgcgcgagaa ggcgccgttc
     3241 ctgaagattc tcggctctta tccggtggcg gtatcctgaa atgaagatcg atctttgcga
     3301 gctgtcgccg tcgtacgtgc gctcgatcgc gccctaccag ccgggcaagc cgatcgccga
     3361 gctggcgcgg gagatgggcc tggacgaagc cggcatcgtc aagctcgcct ccaacgagaa
     3421 tccgcgcggc atcgggccgc gcacgcgcgc cgcgatcgag gcgtcgatcg ccgacatcgc
     3481 gagatatccg gacggcaacg gcttcgagct gaagcaggcc ctcgccaagc gctacggcgt
     3541 cgacatggcg tcgatcgtgc ttggcaacgg ctcgaacgac gcgctcgagc tggtggcgct
     3601 cgccttcctc gggctggggc gcgccgcggt ctactcgcag cactgcttcg ccgtgtaccc
     3661 gctcgccacc caggcgcgcg gcgcgcgctc gatcgtggtg ccggcgaagg atttcggcca
     3721 cgacctcgag gcgatggcga aggcgatcga cgacgagacc tacgtggcgt ggatcgccaa
     3781 ccccaacaat cccaccggaa cctatgcaaa aggcgaagcg gtggaagcgt tcctgcgccg
     3841 cgtgccggag cgcgtgctgg tggtgctcga cgaggcctac aacgagtacc tgccgcacga
     3901 gctcaaggcc gacagcgcgc gctggctgaa gcgccatccg aacctcgtca tcacgcgcac
     3961 cttctcgaag gcctacgggc tcgccggcct gcgcgtgggc tatgcgctcg cccatccgtc
     4021 ggtggccgac gtcatgaacc gcgtgcgcca gccgttcaac gtcaacagca tggcgctcgc
     4081 cgcggcgacg gcggcgctcg acgacatgga gttcgtcgcg cgcagctacg ccgagaacct
     4141 gcagggcctg cgccagatcg aggagggcgc gcgccgcctc ggcctcgact tcatcccgtc
     4201 ctacggcaac ttcatcaccg tgcgcgtcgc gcagggtgga aaatcagcgg cggaaatcta
     4261 caagcggctc ctgcggcgca gcgtcatcgt gcgtccggtc ggcggcggct acggcctgcc
     4321 cgagcacctg cgcgtcacgg tcggcaccgc gccggagaac gagaagttcc tcggcgcgct
     4381 cgccgcgagc ctgaaggact gatgctgttt cagcgcgtcg cggtcgtcgg cgtcggcctg
     4441 atcggcggct cgttcgcact ggccctgaag caggccgcgg cctgcggcca cgtggtcggc
     4501 gtcggccgca acccggtcaa tctgcgcctc gcgctcgagc gcggcatcat cgattctatg
     4561 gagagcgatg cggcgcgcgc cgccgagggt gccgacctgg tgctgctggc cgcgccggtc
     4621 ggccagttcc cgaagatctt cggcagggtc ggcgctgcgc tcaagcccgg cgcgatcctc
     4681 accgacgccg gcagcaccaa gcgcgacgtg gtggcggctg cgcgcaaggc gctgaagaag
     4741 aagatcgccc agttcgtgcc ggcgcatccg atcgccggcg ccgagaagag cggcgcgggc
     4801 gcggcgaacg cggagctgtt ccgcggccgg cgcgtggtga tcacgccgct caaggagaat
     4861 ccggcgcagg cggtcgagaa ggtcaaccag gcgtggtcga cctgcggcgc gcgcaccagc
     4921 cagatgaccc cggagcagca cgacgcggtg ttcgccgcgg tgagccacct gccgcacctg
     4981 ctcgcctacg tgctggtgca cgacgtcgcc ggccgcaaga acgcgaacga gctgttctcc
     5041 ttcgccgccg gcggcttccg cgacttcacg cgcatcgcct cgagccaccc cgagatgtgg
     5101 cgcgacatct gcgtcgccaa cgccgatcgc atcggcgccg agctcgcgag ctacacgaag
     5161 aagctcgccg gcgtgaagaa actcctcgcg cgccgcgatg cgcgcgccct ggagaagctg
     5221 ttcgacgagg cgcgcgacgc gcgcgagagc tggctgcaga agacctgggc cggccgcaag
     5281 cccggcagct gatggagact ctgcgcctcg agcccgtccg ccgtgccgcg ggcgcggtgc
     5341 ggctgcccgg ctcgaagagc atctccaacc gcgtcctgct gctcgccgcc ctggccaaag
     5401 gcgagaccga gatcggcggc ctgctcgatg ccgaggacac gaaagtcatg cgcgaagcgc
     5461 tttcacgcct tggaattgtt ttcaaaggcc agacgatcgt cggagcgggc ggagcgttcc
     5521 cggtacgcaa agcagattta ttcctgggaa atgcaggaac cgcattccgc ccgctcacgg
     5581 cagcgctggc gttctgcaac ggcgaatacc ggctctccgg cgtgccgcgc atgcacgagc
     5641 ggccgatcgg cgatctggtc gacgcgctgc gcgggatcgg tgcgcggatc gattacttgc
     5701 gtaccgacgg gttccctccc ctggcgctgc atccgtcccg attgaaaacc gagaacgtga
     5761 aagtgagagg agatgtttc
//
LOCUS       DASYJA010000238         2576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2759681, whole genome shotgun sequence.
ACCESSION   DASYJA010000238 DASYJA010000000
VERSION     DASYJA010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2759681"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VGO02_05055"
     CDS             <1..411
                     /locus_tag="VGO02_05055"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEV7477091.1"
                     /translation="KMTLPTEIQAGLCGTAEITVGEEHTAPRVGSGRVAVLATPVMIN
                     LIEAAALAAIENLLPEGYQSLGTVLNVRHIAATPVGMKARALVEVVKVEGRTVFFSVE
                     CKDEKELIGDGRHERVVVNVAKFAQRVQRKLEQP"
     gene            408..1499
                     /locus_tag="VGO02_05060"
     CDS             408..1499
                     /locus_tag="VGO02_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477092.1"
                     /translation="MKTAWMVLAAAMLLAAGCQHDPVRGAGAPQFKVDPYWPKPLPSN
                     WILGQVAGIAVDKDDRIWIVHRPATLVDDEKGALANPPATKCCTPAPPVLQFDAQGNL
                     LKSWGGKGSGFDWPESEHGIFVADDGNVWLAGNGAKDQQILQFTPEGKFLRQLGKPGN
                     VGGSNSPGDLGRPAHMVINSGELYVADGYANRRIVVYDAATLAYRRHWGAYGERPHDD
                     KLAPYDPRVALARSFGNPVHCVRVSNEGLVHVCDRANDRIQVFEKSGKFVKEYRVEPQ
                     TLQNGSVWDLVLSEDRAQRYIFVADGANNQIVVLDRASGETLSRFSRGGRMAGELKWV
                     HNMAIDSRGNLYTAEVGTGRRAQKFLRVE"
     gene            complement(1544..2500)
                     /locus_tag="VGO02_05065"
     CDS             complement(1544..2500)
                     /locus_tag="VGO02_05065"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7477093.1"
                     /translation="MKKWIGPALLAFACAAQAQTFPAKPLRMLVGFPPGGTNDLMARI
                     VAQKLGESLGQPVVVENRPGASGVIAAEVLARAAPDGYTLMLGSTGTNTIAPALNAKL
                     PYDPVRDLAPVGLVGIAASALVVNAQVPAHNVAELIALARSRPGKLTYASSGSGTTLH
                     LGGELFKLLAGVDMLHVPYKGNAQALNDVAGGQVDLIFSALPPALPLAKAGRVRILGV
                     GMPERLRSAPELPTIAEQGLPGYEMWTWYGVFATGGSPADAIERLSRELRAALADAKV
                     REQITAQGADPSGSTPAEFRTLVAAELEKWARVVKSAGIKAE"
BASE COUNT          436 a          848 c          921 g          371 t
ORIGIN      
        1 aagatgacgc ttccaacaga aatacaagca gggctatgcg gcactgcgga aatcaccgtc
       61 ggcgaggagc acacagcgcc gcgcgtcggc agcggccggg tggccgtgct ggcgacaccg
      121 gtgatgatca atctcatcga agcggcagcg ctcgccgcga tagagaacct gctgcccgag
      181 ggttaccaga gcctgggcac ggtgctgaac gtgcggcaca tcgccgccac cccggtcgga
      241 atgaaggcgc gggcgctggt tgaggtggtc aaggtggaag gccggacggt attcttctcg
      301 gtcgagtgca aggacgaaaa agagctgatc ggcgacgggc gccacgagcg ggtggtggtg
      361 aacgtcgcga aattcgcgca acgagtgcaa cgcaaactgg agcagccatg aaaaccgcct
      421 ggatggtgct tgccgcagcg atgcttcttg cagccggatg ccagcatgac cccgtgcgcg
      481 gcgccggcgc gccgcagttc aaggtcgacc cctactggcc gaagccgctg ccgtcgaact
      541 ggatcctcgg ccaggtggcg ggcatcgccg tcgacaagga cgaccgcatc tggatcgtgc
      601 accggcctgc cactctggtc gatgacgaaa agggcgcgct cgccaatccg ccggcgacca
      661 agtgctgcac gccggcgccg ccggtgctgc agttcgacgc gcagggcaac ctgctgaaga
      721 gctggggcgg caagggcagc ggcttcgact ggccggagag cgagcacggc atcttcgtcg
      781 ccgacgacgg caacgtctgg ctcgccggca acggcgcgaa ggaccagcag atcctgcagt
      841 tcacgccgga gggcaagttc ctgcgccagc tcggcaagcc gggcaacgtg ggcggctcca
      901 acagcccggg cgacctcggc cgcccggcgc acatggtgat caattcgggc gagctgtatg
      961 tcgccgacgg ctatgcgaac cggcggatcg tcgtgtatga cgccgccacg ctcgcctacc
     1021 gccgccattg gggcgcgtac ggcgagcggc cgcacgacga caagctggcc ccatacgacc
     1081 cgcgcgtggc gctcgcgcgc tcgttcggca acccggtgca ctgcgtgcgc gtctcgaacg
     1141 agggcctggt ccacgtctgc gaccgcgcca acgaccgcat ccaggtgttc gagaagagcg
     1201 gcaagttcgt caaggagtac cgcgtcgagc cgcagaccct gcagaacggc tcggtgtggg
     1261 acctggtgct gtccgaggac cgcgcgcagc gctacatctt cgtcgccgac ggcgcgaaca
     1321 accagatcgt ggtgctcgac cgcgcctcgg gcgagacgct gtcgcgcttc agccgcggcg
     1381 gccgcatggc cggcgagctg aagtgggtgc acaacatggc gatcgactcg cgcggcaacc
     1441 tgtacaccgc cgaggtcggc accggacggc gcgcgcagaa attcctgcgc gtagagtaat
     1501 tctcaggggt ggaaagaaag gaccgcgccg aagacggcca gactcattcg gccttgatgc
     1561 cggcgctttt tacgacgcgg gcccacttct cgagctcggc tgcgaccagc gtgcggaatt
     1621 ccgccggcgt gctgcccgat gggtcggcgc cctgcgcggt gatctgctcg cgtaccttgg
     1681 cgtcagccag cgctgcgcgc agctcgcgcg aaagccgctc gatcgcgtcg gccggcgagc
     1741 cgccggtggc gaagacgccg taccaggtcc acatctcgta gccgggcagg ccctgctcgg
     1801 cgatggtcgg cagctcgggc gcggaacgca gccgctcggg catgccgacg ccgaggatcc
     1861 tcaccctgcc ggccttggcg agaggcagcg cgggcggcag ggcggaaaag atcaggtcga
     1921 cctggccgcc ggcgacgtcg ttcagagcct gcgcgttgcc cttgtagggc acgtgcaaca
     1981 tgtcgacgcc ggcgagcagc ttgaatagct cgccgccgag gtgcagcgtg gtgccgctgc
     2041 ccgacgaggc gtaggtgagc ttgccggggc gcgatctcgc gagcgcgatc agctcggcga
     2101 cgttgtgcgc ggggacctgg gcgttgacca ccagcgcgct cgccgcgatg cccacgaggc
     2161 cgaccggcgc gaggtcgcgc accgggtcat acggcagctt cgcgttgagg gccggcgcga
     2221 tggtattggt gccggtcgag ccgagcatga gcgtgtaccc gtcgggcgcg gcgcgggcga
     2281 ggacctccgc cgcgatgacc cccgaggcac ccggccggtt ctccacgacg accggctggc
     2341 cgagcgattc accgagtttc tgcgccacga tccgcgccat gaggtcgttg gtgccccccg
     2401 gcggaaatcc caccagcatg cgcagcggct ttgctggaaa ggtctgggcc tgcgccgcgc
     2461 aggcgaatgc gagcagtgcc ggcccgatcc actttttcac cggccaagag taccctgtgc
     2521 gtaagggggg gtcatcatgc gtgcacagct agtagcgctt gcgctgggag ccggct
//
LOCUS       DASYJA010000239         2013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_36352, whole genome shotgun sequence.
ACCESSION   DASYJA010000239 DASYJA010000000
VERSION     DASYJA010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36352"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VGO02_05070"
     CDS             <1..250
                     /locus_tag="VGO02_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HEV7477094.1"
                     /translation="DASVSIHQDARVYAGLFSGAEQASYALPRGRLGYVHVARGGVSI
                     NGQKLAAGDALKTDAGEIRIEGGDQAEVLLFDLPYRAD"
     gene            312..782
                     /locus_tag="VGO02_05075"
     CDS             312..782
                     /locus_tag="VGO02_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HEV7477095.1"
                     /translation="MPIDTGISAKDRAAIAGGLSRLLADTYVLYLKTHGFHWNVEGPM
                     FQTLHQMFMDQYTETWNAIDPIAERIRALGHYAPGSSQQYAKLASIKESPGVPKAEKM
                     VRELIEGQEAVARTARSVLPLADKANDQPTLDLLTQRLDIHEKNAWMLRSLLKK"
     gene            809..>2013
                     /locus_tag="VGO02_05080"
     CDS             809..>2013
                     /locus_tag="VGO02_05080"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEV7477096.1"
                     /translation="MSGKRTEKDSFGPIEVPAERLWGAQTERSRRFFRISGERMPLPV
                     IYALALVKKAAAAVNAELGLLEKQKAQAIARAADEVLAGKHDEEFPLVVWQTGSGTQS
                     NMNVNEVLGNRASELLGGERGQKRLVHPNDDVNLGQSSNDVFPTAMHIAAARAMEETL
                     IPAVEALRDTLAAKSRAFSGVVKIGRTHLQDATPLTLGQEFSGYASQLEQAVAALRAT
                     LPGLHQLALGGTAVGTGLNTHPEFSGKVCALVAAETGQPFVPAQNKFAALGGHEALLF
                     AHGALKTLAAAASKIANDVRLLASGPRSGLGELRIPENEPGSSIMPGKVNPTQAEALT
                     MLCAQVLGNDVAINLGGASGQLELNVYKPMISYAFLQSARLLADGSRSFDEHCARGIE
                     PDAARIRELL"
BASE COUNT          360 a          695 c          675 g          283 t
ORIGIN      
        1 ggacgcctcg gtcagcatcc accaggacgc gcgcgtctac gcagggctgt tcagcggcgc
       61 cgagcaggcg agctatgcgc tcccgcgagg gcgcctgggc tacgtgcatg tcgcgcgcgg
      121 cggcgtttcc ataaacgggc agaagctcgc cgcgggcgac gcgctgaaga ccgacgccgg
      181 cgagatccgc atcgagggcg gcgaccaggc cgaggtgctg ctcttcgacc tgccctatcg
      241 ggctgattga aacaaagtag tggtgcgccc gttttcggct ccttacactt ggatgcaaag
      301 gagcagcccc catgcccatc gataccggaa tctccgccaa ggaccgcgcc gcgatcgccg
      361 gcggcctgtc ccggctgctt gccgacacgt atgtcctcta tctcaagacg cacggcttcc
      421 actggaacgt ggaagggccg atgttccaga cgctgcacca gatgttcatg gaccagtaca
      481 cggagacctg gaacgcgatc gatccgatcg ccgagcgcat ccgcgcgctc ggccattacg
      541 cccccggcag ctcccagcaa tacgcgaagc tcgcctccat caaggagagc ccaggcgtgc
      601 cgaaggcgga gaagatggtg cgcgagctga tcgagggcca ggaagccgtg gcgcggaccg
      661 cgcgctcggt cctgccgctc gccgacaagg cgaacgacca gcccacgctc gatctcctca
      721 cgcagcggct cgacatccac gagaagaacg cgtggatgct gaggagcctg ctcaagaagt
      781 gaaatgctga cgtaacattg tggccgcgat gagcggcaag cgcaccgaga aggacagctt
      841 cgggccgatc gaggttccgg ccgagcgcct gtggggcgcg cagaccgagc gcagccgccg
      901 cttcttccgc atctccggcg agcgcatgcc gctgccggta atctacgcgc tcgcgctggt
      961 gaagaaagcc gccgcggcgg tgaatgcgga gctgggcctg ctggaaaaac aaaaggccca
     1021 ggccatcgcg cgcgctgcgg acgaggtgct cgccggcaag cacgacgagg agttcccgct
     1081 ggtggtgtgg cagaccggct ccggcacgca gagcaacatg aacgtcaacg aggtcctcgg
     1141 caaccgtgcc tccgagctgc tcggcggcga gcgcgggcag aagcgcctgg tacacccgaa
     1201 cgacgacgtc aatctcggcc agtcgtcgaa cgatgtcttc ccgaccgcga tgcacatcgc
     1261 ggccgcgcga gcgatggagg agacgctgat acccgcagtc gaggcgctgc gcgacaccct
     1321 cgcggcgaag agccgcgcgt tctcgggcgt ggtcaagatc ggccgcaccc acctgcagga
     1381 tgcgacgccg ctgaccctgg ggcaggagtt ctccggctat gcctcgcagc tcgagcaggc
     1441 ggttgcggct ctgcgcgcca cgctgcccgg cctgcatcag ctcgcgctgg gcggcaccgc
     1501 cgtaggcacc ggcctgaaca ctcatccgga attctcgggg aaagtgtgcg cgctggtggc
     1561 tgccgagacc ggccagccgt tcgtccccgc gcaaaacaag ttcgccgcgc tcggcgggca
     1621 cgaggcgctg ctgtttgccc acggcgcgct gaagacgctc gccgccgctg cgtcgaagat
     1681 cgccaacgac gtgcgcctgc tcgcatccgg accgcgctcc ggcctgggcg agctgcgcat
     1741 cccggaaaac gagcccggca gctcgatcat gccgggcaag gtaaatccca cgcaggccga
     1801 ggcgctgacg atgctctgcg cgcaggtcct cggcaacgac gtcgcgatca acctcggcgg
     1861 cgcttcgggc cagctcgagc tcaacgtcta caagccgatg atcagctacg ccttcctgca
     1921 gagcgcgcgg ctgctggccg acggcagccg ctccttcgac gagcactgcg cgcgcggcat
     1981 cgagcccgac gcggcgcgca tccgcgagct gct
//
LOCUS       DASYJA010000240         2943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_855112, whole genome shotgun sequence.
ACCESSION   DASYJA010000240 DASYJA010000000
VERSION     DASYJA010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855112"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1898)
                     /locus_tag="VGO02_05085"
     CDS             complement(<1..1898)
                     /locus_tag="VGO02_05085"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV7477097.1"
                     /translation="MKVLGKARRRVDGRAKVTGLTRFADDLVLPRMLHMRLLRSPHPH
                     ARIKEINIEKAQKYPGVHLVLTGKDFPVTFGILPVTQDEYPLAPEHVRYVGDPIAAVA
                     AKDEQTAGEALDLIEVKYEILKTISSPEEALKNPEPRIHAYSELGNIHRLQAFEFGDV
                     EESFRKSDLVVEDLFFYEGNTHLPIEQHASLAAVDGEGKLTLWSSTQVPHYVHRCLAR
                     VLQMSAAHIRVVAQPNGGGFGGKCDVCNHEMVVAKAALLLGRPVKICLNREEVFYMHR
                     GRHPVLMRMKTGVTKDGKLTGMHLQTLVDGGGYGSYGVASTFYTGALQTVTYELPRYK
                     FDAARVFTNKPPCGPKRGHGTPQPRFGQEIQLDKIAEKLGRDPAELRLQNVLKPNSLT
                     ANWLKVGTIGLAECIRQVVARSDWSNRFKKLPNGKGLGIACGSYLCGAGLPIYWNKMP
                     QSGVQLLLDRSGQVTVFCGATEIGQGSDDVLAAIVAEVLGIDPFEIRCITGDTGITPV
                     DLGSYSSRVTVMMGNAAIDASNKLKTLIAEAAAEQLEVSPERLQFSDGRVFFEGKTLT
                     FAEAVGFAEEKFGTLGAVGSYKPPRAPGRFKGAGVGPSPAYSYSACVVEVDVDPKTGW
                     IHVPKIWIA"
     gene            complement(1882..2367)
                     /locus_tag="VGO02_05090"
     CDS             complement(1882..2367)
                     /locus_tag="VGO02_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEV7477098.1"
                     /translation="MRRNVQLNLSVNGETVEASFAPYKTLLEVLREDLALTGTKHGCE
                     LGECGACALLVDGEPQLSCLLLAVECEGRHIETVEGLAQGPELHPLQAAFADLGAAQC
                     GYCTPGILMTAKALLEHEKSPSRERITEAISGNLCRCTGYQQIFEAIEEAARRINESP
                     R"
     gene            2523..>2943
                     /locus_tag="VGO02_05095"
     CDS             2523..>2943
                     /locus_tag="VGO02_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEV7477099.1"
                     /translation="MTLRIGDEAPNFKAETTQGSIDFHQWIGNGYAVLFSHPKDFTPV
                     CTTELGTMAKMKTEFDKRNTKIIGLSVDPVNDHERWAKDIEETQGTAPNYPLIGDPDL
                     KISKLYDMLPAEANPGKRTAADNQTVRTVFVIGPDKKV"
BASE COUNT          501 a          972 c          943 g          527 t
ORIGIN      
        1 gctatccaga tcttcggcac gtggatccag ccggtcttcg ggtcgacgtc gacctcgacc
       61 acgcaagcgc tgtacgaata cgccggcgac gggccgacgc ccgcgccctt gaagcgcccc
      121 ggcgcgcgcg gcggcttgta cgagccgacc gcgccgagcg tgccgaactt ctcctcggcg
      181 aagccgacgg cttccgcgaa cgtcagcgtc ttgccttcaa agaataccct gccatccgag
      241 aactgcaggc gttcgggaga gacctcgagc tgctcggctg ccgcctcggc aattaatgtc
      301 tttaatttgt tggaagcatc tatggctgcg ttacccatca tcaccgtcac ccggctcgaa
      361 taggagccga ggtcgaccgg cgtgatgccg gtgtcgccgg tgatgcagcg gatctcgaac
      421 gggtcgatgc cgagcacctc ggcgacgatc gccgccagca cgtcgtcgga gccctggccg
      481 atctcggtcg ccccgcagaa cacggtcacc tggccgctgc ggtcgagcag cagctgcacg
      541 cccgactgcg gcatcttgtt ccagtagatc ggcaggccgg cgccgcacag gtaggagccg
      601 caggcgatgc ccaggccttt tccattcggc agcttcttga acctgttgct ccaatcggaa
      661 cgggcgacga cctgtctgat gcactcggcc aggccgatgg tgccgacctt cagccaattg
      721 gcggtgagcg aatttggttt aagcacgttc tgcaagcgca attcggcggg atcccggccg
      781 agcttctcgg caatcttgtc gagctggatc tcctggccga agcgcggctg cggcgtgccg
      841 tgaccgcgct tcgggccgca gggcggcttg ttggtgaaga cgcgcgccgc gtcgaacttg
      901 tagcgcggca gctcgtaggt cacggtctgc agcgcgccgg tgtagaaggt gctggccacg
      961 ccgtaggagc cgtagccgcc gccgtcgacc agggtctgca ggtgcatgcc ggtgagcttg
     1021 ccgtccttcg tcacgccggt cttcatcctc atgagcaccg ggtggcgccc gcggtgcatg
     1081 tagaacactt cctcgcggtt caggcagatc ttgaccggcc ggccgaggag gagcgccgcc
     1141 ttggccacca ccatctcgtg gttgcagacg tcgcacttgc cgccgaagcc gccgccgttg
     1201 ggctgcgcga ccacccggat atgcgcggcg ctcatctgca gcactcgcgc gaggcagcgg
     1261 tgcacgtagt gcggcacctg cgtcgaggac cacagcgtga gcttgccctc gccgtccacc
     1321 gccgcgagcg aggcgtgctg ctcgatcggc aggtgcgtgt tgccttcgta gaagaacagg
     1381 tcctcgacca ccaggtccga tttcctgaag gattcctcga catcgccgaa ctcgaacgcc
     1441 tgcaggcggt ggatgttgcc gagctcgctg taggcgtgga tccgcggttc gggattcttc
     1501 agcgcctcct cgggactcga gatggtcttc aggatctcgt atttcacttc gatgagatcc
     1561 aatgcttctc ccgccgtttg ctcgtccttc gccgctaccg cggctatcgg gtcgccgacg
     1621 tagcgcacgt gctcgggcgc gagaggatat tcgtcttgcg taacggggag gatgccgaac
     1681 gtgacgggga aatccttgcc ggtcaggacg agatgaacgc ccgggtattt ctgcgctttc
     1741 tcaatgttga tttctttgat ccgtgcatgc ggatgcggag aacgcagcag ccgcatgtgc
     1801 agcatgcgcg gcaggacgag gtcgtcggcg aaacgcgtca ggccggtgac cttggcgcgg
     1861 ccgtcgacgc ggcggcgcgc cttaccgagg actttcattg attctccggg cagcttcttc
     1921 gatggcctcg aatatctgct ggtagccggt gcagcggcag agatttccgg agatggcctc
     1981 ggtgatgcgc tcgcgcgacg gactcttctc gtgctcgagc agcgccttgg cggtcatcag
     2041 gatgcctggg gtgcagtagc cgcactgcgc ggcgccgagg tcggcgaacg cggcctgcag
     2101 cggatggagc tcgggacctt gcgccagccc ctccacggtc tcgatgtggc gcccctcgca
     2161 ctccaccgcg agcagcaggc aggagagctg cggctcgccg tcgaccagca gcgcgcaggc
     2221 accgcattcg ccgagctcgc agccgtgctt ggtgccggtg agcgcgaggt cctcgcgcag
     2281 cacctcgagc agcgtcttgt acggggcgaa ggacgcctcg accgtctcgc cgttgacgct
     2341 caggttgagc tgcacgttgc gccgcaagca tcgactccgg tcgggtggcc cgcgattgtt
     2401 cgtaccccga acaaatccgg cgcctactct actccaaagc ccctctggcg cacagggtcg
     2461 gcgcggcagt aagatcgcac gccacgaccc ccttttacgt cgttcccccg gagagtaccc
     2521 atatgacgct acgcatcggc gacgaagccc ccaatttcaa ggccgaaacc acgcagggct
     2581 cgatcgactt ccaccagtgg atcggcaacg gctacgcggt gctgttctcg cacccgaagg
     2641 atttcacccc cgtgtgcacc accgagctcg gcaccatggc caagatgaaa acggagttcg
     2701 acaagcgcaa caccaaaatc atcggcctct cggtcgaccc cgtgaacgac cacgagcgct
     2761 gggcgaagga catcgaggag acgcagggca cggcgccgaa ctatccgctc atcggcgacc
     2821 cggatctgaa gatctccaag ctctacgaca tgcttccggc cgaagccaac ccaggcaagc
     2881 gcaccgcggc ggacaaccag accgtgcgca cggtgttcgt catcgggccc gacaagaagg
     2941 tca
//
LOCUS       DASYJA010000241         9557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_944929, whole genome shotgun sequence.
ACCESSION   DASYJA010000241 DASYJA010000000
VERSION     DASYJA010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9557
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944929"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="VGO02_05100"
     CDS             complement(<1..139)
                     /locus_tag="VGO02_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011657015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEV7477100.1"
                     /translation="MTTSKQSTAALTLLALGVVYDDIGTSPLYAVKETFNPAHGIPLT
                     PA"
     gene            235..1299
                     /locus_tag="VGO02_05105"
     CDS             235..1299
                     /locus_tag="VGO02_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEV7477101.1"
                     /translation="MKGYVWALVATAACTLAGLALRPHVDPVNVAMLYLLPVVIVALR
                     YSRGAAAAAAVLGVLAFDFLFVPPYLSFAVNDVQYLVTFITMLAVGLVISGLAENARR
                     AARARAAAALEVETERMRSALLSSISHDLRTPLAVMSGASSSLVESGERLAPAERRAL
                     AKSIFQQSSAMAEVMGNVLQMTRLETGAIALKRDWASLGEIAGTVLDRLSGPLARHRV
                     MLDLPADLPLVRVDATLIEQALGNLLENAAKHTPAGTLVRLRAAARDAELTVSVEDFG
                     PGLLPEDFKRVFAKFHRGAAEGSAGGIGLGLAICRAIVALHQGQIWAEQLPGGGTAFR
                     FTLPLEAAPALPVEEAAQEA"
     gene            1296..1994
                     /locus_tag="VGO02_05110"
     CDS             1296..1994
                     /locus_tag="VGO02_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7477102.1"
                     /translation="MSEPRPAILVVEDEPEIRRFLRASLAAEGYRVVEAANGERGAID
                     AGTHKPDMAIVDLGLPDLDGIEVIRRIRAWSTMPIVVLSARVQERSKIEALDAGADDY
                     ITKPFGVGELLARVRVALRNRARSGSGEKILTLGKASIDLERRRAIRDGAEVRLTPVE
                     FKLLVCLARHLGLVVTHRQLLGEVWGPAHTEHTHYLRVYMKGLREKLEADPARPKYLI
                     TETGVGYRLLAEES"
     gene            complement(1991..3880)
                     /locus_tag="VGO02_05115"
     CDS             complement(1991..3880)
                     /locus_tag="VGO02_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEV7477103.1"
                     /translation="MDQDDQARKSTRAALTLAALGIVFGDIGTSPLYAVKETFSAEHG
                     IPLTAENVVGGISTIFWALMIVVSLKYVMLIMRADNRGEGGIMALIALASTAVKDNPH
                     WRLPLLLIGCFGASLFYGDAVLTPAISVLSAVEGLEVGTQAFKPYVVPIAVVVLLALF
                     ALQASGTAAVGRLFGPVTMLWFVAIGAAGIHGIVQHPGILDALNPMHALAFVTGHGLA
                     SFIVLGAVVLAVTGAEALYADMGHFGKGPVRIAWFSLVGPALVLNYFGQGAVLIADPE
                     AVRNPFYLLLPEWALYPMVALATAATVIASQAVISGAYSLTKQAVQLGFLPRMTIVQT
                     SARQIGQIYLPGINWLLCVAVVAAVIGFGSSSRLAGAYGVAVTGTMLVDTLLTFFVIR
                     FGWRYPLWLCIFATGFFLVIDVAFFSATLTKVNDGGWFPLAIGAVVFLIMTTWRRGRE
                     VLFERLSESSVPLQPFLKSLFADPPSRVPGTAVFLTATPDATPHALLHNLNHNKVLHE
                     RVVFLTVETHDVPWVPFEDRVECHRLGHGCWRVTVRYGFMNRPDVVRALDMCGALGLE
                     FDMMQTSFFLSRQQVVPTAAGPSAMALWRERMFAAMARNAANVTDYFNIPTNRVIELG
                     TKVEI"
     gene            complement(3926..6097)
                     /locus_tag="VGO02_05120"
     CDS             complement(3926..6097)
                     /locus_tag="VGO02_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477104.1"
                     /translation="MSKIFDKMKGLERDRQAKVSEQIEELRSRIAAERIAEETVSARG
                     GEERRALDLARERQVSEGELRRIAHARAEAEAKAARVMAERERAEAQALAEARAREDA
                     EKKAAALADGRRQKEEEASQHARDRAEAEAQAAAAMRERAAAEEQARRAARERAQAET
                     RALESVRERKVAEEKLKREADARAAAENEASRIAKERAETEGAAAEQAVSRGEAETRA
                     AAAAEERRSAETQAAQAARGRAEAEEKARLAAEGKIAAERAAAAAVRERETQSAHATR
                     RAQARADAEAAALQAAAERREAEERAAAEAEAKREAEERGAALATERRAMERAADEKA
                     RERAALEQQALEAAERNAAAEKAAQLAAEARTQIEAQAAQLAQEREAADAELKRSARE
                     RAEAESDAAQAAREAAEARVRALAESQARAAAEQAQTAAVAERAEAEEALRKRSAVAA
                     ETDRVAAEAARARAAAEAQARVAAEASARAQAEAAQRAREREQAEIAAEAALRGRLDA
                     EERKRAAAAELAAAEAAAAAELQARIAAEKELQDDAQSAAAAEKEALATARQREALDG
                     ELRRAAQARAEADEEAVQRAREREQVEARVAAGLHARLDAERSAEALSMERVEAETRT
                     TATLQARIAAEEALAAKMAEASAAAQLATEAKGVRVAHQQELKRVRVGAPSRRWAWVA
                     VFVAAAAFIGFLWYEKPAAPPVSAAASGEPLKLKLERELRK"
     gene            complement(6094..7218)
                     /locus_tag="VGO02_05125"
     CDS             complement(6094..7218)
                     /locus_tag="VGO02_05125"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEV7477105.1"
                     /translation="MSDAGKPIILAVDDDSDLLALIGKVLSRDYKVLTAEDPGTALDH
                     AVGKPRPDLILLDVEMPGASGFELFQVLKAEANVTDIPIIFLTGNSEARSQVEGLEMG
                     AVDYITKPINAAVLQVRVRTQMALANRRSELERLVKERTAALESTRLQLIRRLSRAME
                     LHESAATGNRVMRLSQYARLIAHAAGAKPAVSEMLSIAAPLHDIGKLGVPAEILRKTG
                     KLSAPDWERVKRHPEIGADIIGEHDDPLLKLARQLALTHHERWDGGGYPKGLKGELIP
                     WAGRLMAVVDAFESMTTTQFWREPLKFMEAANEIMEGSGKRYDPKIVEAFRQALPHLK
                     KVREAYNDSLGDMINLDFSPGRGAIDRTGKGGKRLGARLK"
     gene            complement(7218..7934)
                     /locus_tag="VGO02_05130"
     CDS             complement(7218..7934)
                     /locus_tag="VGO02_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEV7477106.1"
                     /translation="MAFAVLCALANFVWADEITVLAGGAARHALEPVAATLQGHKVSF
                     DFQTMGRLQQSLAAGQKADLVVVSSEVLERLEKDGKVPAGRGTPLARVGIGVAVHEKA
                     PLPDISTPEAMRRTLLAAKSIVYINPKTGTSGKYVEDMLSKLDILGDMKSKTTLIDEG
                     YAVAPVGRGEVELGIHQISEIIPVPGVKLVGELPAQFQRYTVYVAVPLNESKAVKDFI
                     AHATGPQARERLARAGYTSP"
     gene            complement(7940..>9557)
                     /locus_tag="VGO02_05135"
     CDS             complement(7940..>9557)
                     /locus_tag="VGO02_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEV7477107.1"
                     /translation="FALHCAERLAHGLEQLSAGETVLVLLDLSLPDSFGLETFAKVYA
                     HSPTVPIIVLTGNDDQTVALSAVKGGAQDYLIKGRLDRELLLRSMQYSIERKRYQVQL
                     EHQANYDALTGLPNRNLLHDRLKQAVYAQRVPRAIAVVFIDLDHFKFVNDSLGHSTGD
                     KLLKGMADRLRGVLREGDTVARLGGDEFVLILNDQSNEEVIFRAMQRITQQVSQPMDI
                     DGKELYLTCSAGISLYPQDGPDVDTLLKNADAAMYRAKEHGRNNFQFYTSEMNERVNE
                     RLQLENALRRALERKEFLLHYQQRVDLKSGVLLGAEALVRWMHPEWGLVRPARFIALA
                     EETGLIVPIGEWVLREACRQNRAWIEQGLNPGFVSVNLSTRQFRQEGLVRTVSRILEE
                     TGLDPQLLEMEITESMVMHNVAAAVATLQGLKSLGVGLSVDDFGTGYSSLAYLKDLPI
                     DALKIDRSFVRDIGTGDNAQDGVLAAAIVTLGHSLHLKVVAEGVETDSQVRFLKRHRC
                     DEVQGFFYSEPLTPEAYAGSLGKPKAKGRK"
BASE COUNT         1253 a         3462 c         3332 g         1510 t
ORIGIN      
        1 tggccggggt gagcgggatg ccgtgcgccg ggttgaaggt ttccttgacc gcgtacagcg
       61 ggctggtgcc gatgtcgtcg tagacgacgc cgagagcgag aagagtcagg gctgccgtgc
      121 tctgcttgga ggttgtcatg agggcgcgca gcttagccgc gccagcgccc gccgcaccgg
      181 gtgacggcgt ttttttacgc cgtctttacg gcagccttgc gctagagtgg acgcatgaag
      241 ggctacgtct gggcgctcgt cgccacggcg gcctgcacgc ttgccgggct ggcgctgcgg
      301 ccgcacgtcg atccggtcaa cgtcgcgatg ctctacctgc tgccggtcgt gatcgtcgcg
      361 ctgcgctaca gccgcggcgc ggcggcggcc gccgccgtgc tcggcgtgct cgcctttgat
      421 ttccttttcg tgccgcctta cctgtcgttc gcggtcaacg acgtgcagta cctggtgacg
      481 ttcatcacca tgcttgcggt cgggctggtg atctcgggtc tcgcggaaaa cgcgcgccgc
      541 gcggccaggg cgcgcgccgc ggccgcgctc gaggtcgaga ccgagcgcat gcgcagcgcg
      601 ctgctctcgt cgatctcgca cgatctgcgc acgccgctgg cggtcatgtc gggcgcttca
      661 tccagcctgg tggaaagcgg cgagcggctc gctccggcgg agcgccgcgc cctcgccaag
      721 agcatcttcc agcagtccag cgcgatggcc gaggtgatgg ggaacgtgct gcagatgacg
      781 cggctggaga ccggcgccat cgcgctcaag cgcgactggg cctcgctcgg cgagatcgcc
      841 ggcacggtgc tcgatcgcct gagcgggccc ctcgcgcggc accgcgtgat gctcgacctg
      901 cccgccgacc tgccgctggt ccgcgtcgac gctacgctga tcgagcaggc gctcggcaac
      961 ctgctcgaga acgcggcaaa gcacacgccg gccgggacgc tggtgcggct gcgcgcggct
     1021 gcgcgcgacg ccgagcttac ggtgtccgtg gaggacttcg gccccggcct cctgccggaa
     1081 gacttcaagc gcgtcttcgc caagttccac cgcggcgcgg ccgaaggcag cgccggcggc
     1141 atcggtctcg gcctggcgat ctgccgggcg atcgtggccc tgcaccaggg ccagatctgg
     1201 gccgagcagc tgccgggcgg cggcaccgct ttccgcttca cgctgccgct cgaggcggcg
     1261 cccgcgctgc cggtggagga agcggcgcag gaagcatgag cgagccgcgg ccggcgatcc
     1321 tggtggtcga ggacgagccc gagatccgcc gcttcctgcg cgcgtcgctc gccgcggaag
     1381 gctatcgggt agtcgaggcg gcaaacgggg agcgcggcgc catcgacgcc ggcacgcaca
     1441 agcccgacat ggcgatcgtc gacctcggcc tgcccgacct ggacggcatc gaggtgatcc
     1501 gccgcatcag agcgtggtcg accatgccca tcgtcgtgct gtccgcgcgc gtgcaggagc
     1561 gctccaagat cgaagcgctc gatgccggcg ccgacgacta catcaccaag cccttcggcg
     1621 tcggcgagct gctcgcgcgc gtgcgggtgg cgttgcgcaa cagggcgcgc tcagggagtg
     1681 gggaaaagat cctgacgcta ggcaaggcct cgatcgacct ggagagacgc cgtgccatcc
     1741 gcgacggcgc cgaggtgcgg ctcacgccgg tcgaattcaa gttgctggtc tgcctcgcca
     1801 ggcatctcgg cctggtggtg acccaccgcc agctgctggg cgaggtgtgg gggccggcgc
     1861 acacggagca cacccactac ctgcgcgtct acatgaaggg actcagggag aagctcgaag
     1921 ccgatcccgc acggccgaag tacctgatca ccgagacagg ggtgggctac cggcttctgg
     1981 cggaggaaag ctagatctct accttggtgc cgagctcgat cacccggttg gtggggatgt
     2041 tgaagtagtc ggtgacgttg gcggcattgc gcgccattgc cgcgaacatg cgctcgcgcc
     2101 agagcgccat cgccgaggga ccggcggcgg tgggcaccac ctgctgccgc gacaggaaga
     2161 acgaggtctg catcatgtcg aactcgaggc cgagggcgcc gcacatgtcg agcgcgcgca
     2221 ccacgtcggg ccggttcatg aagccgtagc gcaccgtcac ccgccagcag ccgtggccga
     2281 ggcggtggca ctcgacgcgg tcctcgaacg gcacccacgg cacgtcgtgc gtctcgacgg
     2341 tgaggaacac cacgcgctcg tgcagcacct tgttgtggtt gaggttgtgc agcagcgcgt
     2401 gcggcgtcgc gtccggcgtg gcggtgagga acaccgcggt gcccggcacg cgcgacggcg
     2461 gatcggcgaa cagcgacttg aggaacggct gcagcggcac cgagctttcg ctgaggcgct
     2521 cgaacagcac ctcgcgcccg cgccgccagg tggtcatgat gaggaacacc acggcgccga
     2581 tcgccagcgg gaaccagccg ccgtcgttca ccttggtgag ggtggcggag aagaacgcca
     2641 cgtcgatcac caggaagaag ccggtggcga agatgcacag ccacagcggg tagcgccagc
     2701 cgaagcggat gacgaagaaa gtgagcagcg tgtcgaccag catcgtgccg gtgaccgcca
     2761 cgccgtacgc gccggcgagg cgcgacgagg agccgaagcc gatcaccgcg gccaccaccg
     2821 cgacgcacag cagccagttg atgcccggca ggtagatctg cccgatctgg cgcgccgagg
     2881 tctgcacgat ggtcatgcgc ggcaggaagc cgagctgcac cgcctgcttg gtaagcgaat
     2941 aggcgccgga aatcaccgcc tgcgaggcaa tcaccgtcgc cgcggtggcg agcgccacca
     3001 tcgggtacag cgcccactcc ggcagcagga ggtagaacgg gtttctcacc gcttccggat
     3061 cggcgatcag caccgcgccc tggccgaaat agttgagcac cagcgccggc cccaccaggc
     3121 tgaaccaggc gatgcgcacc ggtcccttgc cgaaatggcc catatccgcg tacagcgcct
     3181 cggccccggt gaccgcgagc accaccgcgc ccagcacgat gaacgaggcc aggccgtggc
     3241 cggtgacgaa ggcgagcgcg tgcatcggat tgagcgcgtc aaggatgccg gggtgctgca
     3301 cgatgccgtg gatcccggcc gcgccgatcg ccacgaacca cagcatggtg accggtccga
     3361 acaggcggcc gacggccgcc gtgccgctcg cctgcagcgc gaacagcgcc agcagcacga
     3421 cgaccgcgat gggcacgaca taaggcttga aggcctgggt gccgacctcg agcccttcca
     3481 ccgccgaaag caccgagatc gccggcgtca gcaccgcgtc gccatagaag agcgacgcgc
     3541 cgaagcagcc gatgagcagc agcggcaggc gccagtgcgg gttgtccttg accgcggtgg
     3601 aagcgagcgc gatcagcgcc atgatgccgc cctcgccccg gttgtcggcg cgcatgatga
     3661 gcatcacgta cttcagcgac accacgatca tcaacgccca gaagatcgtg gagatgccgc
     3721 ccaccacgtt ctccgcggtg agcgggatgc cgtgctcggc cgagaaggtc tccttgacgg
     3781 cgtagagtgg gctcgtgccg atgtcgccga acacgatgcc cagggcggca agggtcagcg
     3841 cggcacgggt ggatttcctg gcttggtcgt cttggtccaa gagcgggcgc caagctactc
     3901 ccgcactgca caaaatgcaa atcagctact tgcgcagttc gcgttcgagc ttgagcttga
     3961 gcggttcccc ggaggcggcg gcgctcaccg gcggcgccgc cggcttctcg taccagagga
     4021 agccgatgaa cgccgcggca gcgacgaaca cggcgaccca cgcccagcgg cgcgatggcg
     4081 cgccgacgcg cacccgcttg agctcctgct ggtgcgcgac gcgcacgccc ttggcttctg
     4141 tggccagctg cgccgccgcc gaagcctccg ccatcttcgc ggcgagcgcc tcctcggcgg
     4201 cgatgcgcgc ctgcagcgtc gcggtggtcc gcgtctccgc ctcgacgcgc tccatcgaca
     4261 gcgcctcggc cgagcgctcg gcgtcgagcc gcgcgtgcag cccggccgcg accctcgctt
     4321 ccacctgctc gcgctcgcgc gcgcgctgca ccgcttcctc gtcggcttcg gcgcgcgcct
     4381 gcgcggcgcg gcgcagctcg ccgtcgagcg cctcgcgctg gcgcgccgtg gcgagcgcct
     4441 ccttctccgc cgcggcggcg ctctgcgcgt cgtcctgcag ctccttctcc gccgcaatcc
     4501 gcgcctgcag ctcggcagcc gctgcggctt ccgcggcggc cagctcggcc gccgccgccc
     4561 gcttgcgctc ctcggcgtcg aggcgcccgc gcagcgccgc ttccgccgcg atctcggcct
     4621 gctcgcgctc gcgcgcgcgc tgcgccgcct cggcctgggc ccgcgcgctt gcctcggccg
     4681 cgacgcgcgc ctgcgcttcg gccgcggcgc gcgcgcgcgc cgcctccgcc gcgacgcggt
     4741 cggtttcggc cgccacggcg gagcgcttgc gcagcgcctc ttccgcctcg gcgcgctccg
     4801 ccaccgcggc agtctgcgcc tgctccgccg ccgcgcgcgc ctgcgattcg gcgagcgcgc
     4861 gcaccctggc ctcggcggcc tcgcgcgccg cctgcgccgc gtccgactcc gcctccgcgc
     4921 gctcgcgcgc gctgcgcttg agctccgcgt cggccgcttc gcgctcctgc gccagctgcg
     4981 ccgcctgcgc ctcgatctgg gtgcgcgcct cggctgccag ttgcgcggcc ttctcagccg
     5041 ccgcgttgcg ctcggccgcc tcgagcgcct gctgctcgag cgccgcgcgc tcgcgcgcct
     5101 tctcgtcggc cgcgcgctcc atcgcgcgcc gctcggttgc gagcgcggcg ccgcgctcct
     5161 ccgcctcgcg cttcgcctcc gcctccgccg cggcgcgctc ctcggcctcg cgccgttccg
     5221 ccgccgcctg cagcgccgcc gcttccgcgt cggcgcgcgc ctgcgcgcgt cgggtcgcgt
     5281 gcgcgctttg cgtctcccgc tcgcgcaccg ccgccgccgc cgcgcgctcc gcggcgatct
     5341 tgccctcggc cgcgagccgc gctttttcct cggcttcggc gcggccgcgc gccgcctgcg
     5401 ccgcctgcgt ttcggcgctg cggcgctcct cggcggcggc cgccgcgcgc gtctcggctt
     5461 cgccacgcga gacggcctgc tcggccgccg cgccctcggt ctcggcgcgt tccttcgcga
     5521 tgcggctcgc ctcgttttcc gcggcggcgc gcgcgtcggc ctcgcgcttg agcttctcct
     5581 cggccacctt gcgctcgcgc accgactcga gggcgcgcgt ctcggcctgc gcgcgctcgc
     5641 gcgcggcacg ccgcgcctgc tcctcggcgg cggcgcgctc gcgcatcgcc gccgcggcct
     5701 gcgcctcggc ctcggcgcgg tcgcgcgcgt gctgcgaggc ctcctcctcc ttctgccggc
     5761 gcccgtcggc cagcgcggcc gccttcttct ccgcgtcctc gcgcgcgcgc gcctcggcca
     5821 gcgcctgcgc ctcggcgcgc tcgcgctcgg ccatgacgcg cgccgccttg gcctcggcct
     5881 cggcgcgcgc gtgcgcgatg cgccgcagct cgccctcgga cacctggcgc tcgcgcgcca
     5941 gatccagggc gcggcgctcc tcgccgccgc gcgccgacac ggtctcctcg gcgatgcgct
     6001 ccgccgcgat gcgcgagcgc agctcctcga tctgctcgct caccttcgcc tggcggtcgc
     6061 gctcgaggcc cttcatcttg tcgaagatct tgctcacttg agtcgcgccc ccagcctttt
     6121 tccgcccttg ccggtgcggt cgatggcgcc gcgcccgggc gagaaatcca ggttgatcat
     6181 gtcgccgagg gagtcgttgt aggcctcgcg caccttcttc aggtgcggca gcgcctggcg
     6241 gaacgcctcg acgatcttcg gatcatagcg cttgcccgag ccttccatga tctcgttggc
     6301 cgcctccatg aacttcagcg gctcgcgcca gaactgggtg gtggtcatcg actcgaaggc
     6361 gtcgaccacc gccatcaggc gccccgccca ggggatcagc tcgcccttga ggcccttggg
     6421 gtagccgccg ccgtcccagc gctcgtggtg cgtgagggcg agctggcgcg cgagcttgag
     6481 cagcgggtcg tcgtgctcgc cgatgatgtc ggcgccgatc tcgggatggc gcttgacgcg
     6541 ctcccagtcg ggcgccgaca gcttgccggt cttcctcagg atctcggcgg gcacgccgag
     6601 cttgccgatg tcgtgcagcg gcgcggcgat cgacaacatt tcggagactg cgggcttggc
     6661 gcccgccgcg tgcgcgatca ggcgcgcgta ctgcgacagg cgcatcacgc ggttgccggt
     6721 cgccgcgctc tcgtgcagct ccatcgcgcg cgacaggcgc cggatcagct gcagccgcgt
     6781 gctctcgagc gccgcggtgc gctccttcac caggcgctcg agctcgctgc gccggttggc
     6841 gagcgccatc tgggtgcgca cgcgcacctg caggaccgcg gcgttgatcg gcttggtgat
     6901 gtagtcgacc gcgcccatct cgaggccctc gacctgcgag cgcgcctcgc tgttgccggt
     6961 gaggaagatg atcgggatgt cggtgacgtt ggcctcggcc ttgagcacct ggaacagctc
     7021 gaagcccgag gcgccgggca tctcgacgtc gagcaggatc aggtcggggc gcggcttgcc
     7081 caccgcgtgg tcgagcgcgg tgcccggatc ctcggcggtg agcaccttgt agtcgcgcga
     7141 cagcaccttg ccgatgaggg cgaggaggtc gctgtcgtcg tcgacggcga ggatgatggg
     7201 cttaccggca tcgctcacta cggggaagtg tatcccgccc tggccaggcg ctcccgcgcc
     7261 tgcgggccgg tggcgtgcgc gatgaaatcc ttcacggctt tcgattcgtt caggggcacc
     7321 gccacgtaca cggtgtagcg ctggaactgc gccggcagct cgccgaccag cttcacgccc
     7381 ggcaccggga tgatctcgct gatctggtgg atgccgagct cgacctcgcc gcgcccgacc
     7441 ggcgccaccg cgtagccctc gtcgatcagg gtggtcttgc ttttcatgtc tcccaatata
     7501 tctagtttgg aaagcatgtc ttcgacgtac ttgccgctgg tgccggtttt cgggttgatg
     7561 tacacgatcg acttcgccgc gagcagagtg cggcgcatcg cctccggcgt ggagatgtcg
     7621 ggcagcggcg ctttttcgtg caccgccacg ccgatgccga cccgcgccag cggcgtgccg
     7681 cgccccgccg gcaccttgcc gtccttctcc agccgctcga gcacctcgct gctcaccacg
     7741 accaggtcgg ccttctgccc ggcggcgagc gactgctgca gccggcccat cgtctggaag
     7801 tcgaagctca ccttgtggcc ctgcagggtg gcggccacgg gctcgagggc gtggcgcgcc
     7861 gcgccgccgg ccagcactgt tatctcatct gcccagacga aattcgcgag ggcgcagagg
     7921 actgcaaatg ccatgaatct catttcctgc ctttcgcttt gggcttgccg agggacccgg
     7981 cgtacgcctc gggggtcagc ggctcgctgt agaagaagcc ctgcacctcg tcgcagcggt
     8041 ggcgcttgag gaagcgcacc tgcgagtcgg tctcgacgcc ctcggcgacc accttgaggt
     8101 gcaggctgtg gccgagggtg acgatcgccg ccgcgagcac gccgtcctgc gcgttgtcgc
     8161 cggtgccgat gtcgcgcacg aaggagcggt cgatcttgag ggcgtcgatc ggcaggtcct
     8221 tgaggtaggc gagcgacgag tagccggtgc cgaagtcgtc caccgacagg ccgacgccga
     8281 gcgatttgag cccctgcagc gtggctaccg ccgcggcgac gttgtgcatc accatgctct
     8341 cggtgatctc catctcgagc agctgcgggt cgagccccgt ctcctcgagg atgcgcgaca
     8401 ccgtgcgcac caggccttcc tggcggaact gccgcgtcga caggttcacc gagacgaagc
     8461 ccgggttgag cccctgctcg atccaggcgc ggttctggcg gcaggcctcg cgcagcaccc
     8521 attcgccgat cggcacgatc aggccggtct cctcggcgag cgcgatgaag cgcgccgggc
     8581 gcaccaggcc ccactcgggg tgcatccagc gcaccagcgc ctcggcgccg agcagcacgc
     8641 cgctcttcag gtcgacgcgc tgctggtagt gcagcaggaa ctccttgcgc tcgagcgcgc
     8701 ggcgcagcgc gttctcgagc tgcaggcgct cgttgacgcg ctcgttcatc tccgaggtgt
     8761 agaactggaa gttgttgcgc ccgtgctcct tcgcccggta catcgccgcg tcggcgttct
     8821 tcagcagggt gtcgacgtcg gggccgtcct gcgggtacag gctgatgccg gcgctgcagg
     8881 tgaggtagag ctccttgccg tcgatgtcca tcggctggct cacctgctgg gtgatgcgct
     8941 gcatggcgcg gaagatcacc tcctcgttcg actggtcgtt caggatcagc acgaactcgt
     9001 cgccgcccag ccgcgccacc gtgtcgccct cgcgcagcac gccgcgcagg cgatcggcca
     9061 tgcccttgag cagcttgtcg ccggtggagt ggccgaggct gtcgttgacg aacttgaagt
     9121 ggtcgaggtc gatgaacacc accgcgatgg cgcgcggcac gcgctgcgcg tacaccgcct
     9181 gcttgaggcg gtcgtgcagc aggttgcggt tgggcagccc ggtgagcgcg tcgtagttgg
     9241 cctggtgctc gagctggacc tggtagcgct tgcgctcgat cgagtactgc atggagcgca
     9301 gcagaagctc gcggtcgagg cggcccttga tcaggtagtc ctgcgcgccg cccttcaccg
     9361 ccgagagcgc cacggtctgg tcgtcgttgc cggtgagcac gatgatcggc acagtcggcg
     9421 aatgggcgta gaccttggcg aaggtctcca ggccgaagct gtcgggcagc gacagatcga
     9481 gcagcaccag caccgtctcg cccgccgaga gctgctccag gccgtgcgcc agccgctcgg
     9541 cgcaatgcag cgcgaag
//
LOCUS       DASYJA010000242         2074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2941895, whole genome shotgun sequence.
ACCESSION   DASYJA010000242 DASYJA010000000
VERSION     DASYJA010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2941895"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VGO02_05140"
     CDS             complement(<1..263)
                     /locus_tag="VGO02_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7477108.1"
                     /translation="MPAAVPALALEKVTVTFVGQDGGRYTAIRDTTLHVAPGEFVSVV
                     GPTGCGKSTLLNVAVGLLQPSAGSTRVFGETLAGLNRKAGYLF"
     gene            complement(266..1285)
                     /locus_tag="VGO02_05145"
     CDS             complement(266..1285)
                     /locus_tag="VGO02_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7477109.1"
                     /translation="MRLTALAIAAALAASSGAQAQIEKRDVHIAVGGKTALYYLPLTI
                     TERLGYFKDEGLNVRISDFAGGTRSLEAVVGGSADVVAGAYEHTINMQSRRQNFQAFV
                     LAGAAPQISVAISSKLAGKYQSPKDLKGLKVGVSAPGSSTNMVINYVLAQGGLKPDDV
                     AIIGIGQGASVVAAMDQGRVDVISQTDPAVTMLEREGKVKVIAETRTPEGTLKLFGGP
                     MPASSLYAPIEFVKKNPNTVQALTNATVRALQWMQEASPQQILAAVPEEYLLGNQAMY
                     LFAYNNVKTAYSKDGYFSDAGAKTTLKALASFNPNIKPEAINLAATYTNEFVKKANAK
                     YSTKK"
     gene            complement(1314..1928)
                     /gene="recR"
                     /locus_tag="VGO02_05150"
     CDS             complement(1314..1928)
                     /gene="recR"
                     /locus_tag="VGO02_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEV7477110.1"
                     /translation="MKNKDAVPTAKGLELLVEALRVLPGVGPRSAQRMAYHLLQHDRE
                     GGERLAHALAAALGSVRRCVKCNNFTEEELCALCRSPRRDPSLLCVVETPADLSMVEQ
                     TLSYSGMYFVLMGRLSPLDGIGPRDIGLERLLERTAGGEVQEVILATNFTNEGEATAH
                     YIAEMLRARGIRVSRIARGVPLGGELEYVDAGTISQALLERREI"
     gene            complement(1925..>2074)
                     /locus_tag="VGO02_05155"
     CDS             complement(1925..>2074)
                     /locus_tag="VGO02_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV7477111.1"
                     /translation="LVGDDREMLEDLVAAAFNDASHRVEATIAEKMSGMTAGLGLPAG
                     FKLPF"
BASE COUNT          319 a          693 c          683 g          379 t
ORIGIN      
        1 tgaaacagat agcccgcctt gcggttgagc cccgcaagcg tctcgccgaa cacgcgcgtg
       61 gagccggcgg agggctggag cagccccacg gcgacgttca acagggtcga cttgccgcag
      121 ccggtgggcc ctacgacgga gacaaattca cctggcgcga catggagcgt ggtgtcgcgg
      181 atggcggtat agcggccgcc atcctggccg acgaacgtaa cggtgacctt ctccagcgcc
      241 agcgccggaa ccgcggccgg catcgctact tcttcgtcga gtacttcgcg ttcgccttct
      301 tcacgaactc gttggtatag gtcgcggcga gattgatcgc ctcgggcttg atgttcgggt
      361 tgaaggaggc gagcgccttg agcgttgtct tcgcgcctgc gtcgctgaag tagccgtcct
      421 tcgaataggc cgtcttcaca ttgttgtagg cgaacaggta catggcctgg ttgccgagca
      481 ggtactcctc gggaaccgcc gccaggatct gctgcggcga agcctcctgc atccattgca
      541 gcgcgcgcac cgtggcgtta gtcagagcct ggacggtgtt cgggttcttc ttcacgaact
      601 cgatcggcgc atacaggctc gaggccggca tcggcccgcc gaagagcttc agcgtgcctt
      661 ccggcgtgcg cgtctcggcg atcaccttca ccttgccttc cctctccagc atggtgaccg
      721 ccggatcggt ctgcgagatg acgtccaccc tgccctggtc catcgcggcg acgaccgagg
      781 ctccctggcc gatgccgatg atcgccacgt cgtccggctt gaggccgcct tgggcgagga
      841 catagttgat caccatgttc gtgctcgagc ccggcgcgct gacgccgacc ttgagcccct
      901 tcaggtcctt gggcgattga tacttgcccg cgagcttgct cgagatcgcg acgctgatct
      961 gcggcgcggc gccggcgagc acgaacgcct ggaagttctg ccggcgcgac tgcatgttga
     1021 tggtgtgctc gtaggcgccc gccaccacgt cggcgctgcc accgacgacc gcttccagcg
     1081 agcgcgtgcc gccggcgaag tcgctgatcc ggacgttgag gccttcgtcc ttgaaatacc
     1141 ccagccgctc cgtgatggtg aggggcaggt agtacagcgc ggtctttcct cccaccgcga
     1201 tatgaacgtc gcgcttctcg atctgtgctt gggcgccaga gctggcggcg agcgcagcgg
     1261 cgatcgccag ggcggtcagt ctcatgtcgg gtcctcctcc gaagcgcaat ttactagatt
     1321 tcgcggcgtt ccagcagcgc ctgggagatc gtgccggcgt cgacgtactc cagctcgccg
     1381 cccaggggca cgccgcgcgc gatccggctg acgcggatgc cgcgcgcgcg cagcatttcg
     1441 gcaatgtagt gcgcggtggc ctcgccctcg ttcgtgaagt tggtggcgag gatcacttcc
     1501 tgaacctcgc cgccggcggt gcgctccagc agccgctcga ggccgatgtc gcgcggcccg
     1561 atgccgtcga gcggggacag cctgcccatg aggacgaagt acatgccgga gtagctcagg
     1621 gtctgctcca ccatcgacag gtcggccggc gtttccacca cgcagagaag cgaagggtcg
     1681 cgccgcggcg agcggcacag cgcacatagc tcctcttcgg tgaagttgtt gcacttgacg
     1741 cagcgccgta cgctgcccag cgcagcggca agcgcgtgcg cgagtcgctc cccgccttcg
     1801 cggtcgtgct gcaggaggtg atacgccatg cgctgcgccg aacggggccc cactcccggc
     1861 agcacgcgca gcgcttcgac caggagctcc agccccttcg cggtgggaac ggcgtcctta
     1921 ttcttcaaaa cggaagcttg aatcccgcgg gcagcccgag gccggccgtc atgccactca
     1981 ttttctcggc gatggtcgcc tcgacgcggt gcgaggcgtc gttgaacgca gcagcgacca
     2041 ggtcctcgag catctcgcgg tcgtcgccga ccag
//
LOCUS       DASYJA010000243         4753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_308394, whole genome shotgun sequence.
ACCESSION   DASYJA010000243 DASYJA010000000
VERSION     DASYJA010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308394"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VGO02_05160"
     CDS             <1..382
                     /locus_tag="VGO02_05160"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEV7477112.1"
                     /translation="LLARYRQNVDSDRGALADLRLNVGVYGDRGATLVVFSEATWGSS
                     KANATFYGVTPQQSAASGLAVYEPGSGLKHLALGLIGGYDLSRHWTLVASAQQRWLQG
                     DVAKSPLVEKKGNTYANAGIAYRF"
     gene            complement(394..1053)
                     /locus_tag="VGO02_05165"
     CDS             complement(394..1053)
                     /locus_tag="VGO02_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7477113.1"
                     /translation="MVASSVSAAALRGETARFRKPATQKEADILEAATLIFSEKGYQG
                     ARTAEIAEAAGVTERTLFRYFRSKEALFRRVMFPALLAAAMPRALIDAGQLFGADARS
                     FAAWHRNILTVRLDAALDAGPQFRMLVGALMTDEALRRKTVRLWRNNVWKPLLSSMRR
                     FRRRGELRSGLRPETVARAVISLNLGYIVARALLAPEADWDDPAEIEETVQLLLKGAG
                     A"
     gene            1104..1979
                     /locus_tag="VGO02_05170"
     CDS             1104..1979
                     /locus_tag="VGO02_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477114.1"
                     /translation="MRKVRVAPKGMGLNDFVAQQEGFFAAEGIEVEFDIKTFRGTQSS
                     WKGLDYKSRPQDRPYAEGDQGLIQCACAWGSVSNASAGMGRFIPDCYGISPWAIFVRP
                     DSKIRRPADLKDVPISVGMRAGSHFNVPYRLEKHLPLERIKVVNTGGFGARLRALLDG
                     EVEAASLLPPQIDMARQLGLRMVMEDVFHTLWWVPETADPADVRAYLRGLDRAEAALA
                     SDLKKYLPLWEKCVPPEFKDAHRWDFGGFTRGERFVFQPMPRSEFDDVLGQVERWGLD
                     EHLKERSFEKLAFRV"
     gene            complement(1976..2557)
                     /locus_tag="VGO02_05175"
     CDS             complement(1976..2557)
                     /locus_tag="VGO02_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477115.1"
                     /translation="MTIVEWAQFTAIAVCGAFGLFCLFEGTRRLAAHDPRGRASNLVG
                     IGVVVVGMLAGYAYWQHWTYADVARSYQPAETVHELPTDFGKKMSPAKREAASRSAAR
                     GTFITSGKLTQYVDASGQRKLYPPVQDDIKHREDVLATASRLERKASESFVEFILWLI
                     LGLSAVVFGLCFAFEPAAKRADSEADLEAQPPA"
     gene            complement(2609..3364)
                     /locus_tag="VGO02_05180"
     CDS             complement(2609..3364)
                     /locus_tag="VGO02_05180"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7477116.1"
                     /translation="MPLSGLQGKRALVTGAASGIGHAVVEKLKKEQVEVFSTDIHDAH
                     GVIVADLTDADDVEKLAGKVGAIDFLINVAGGPIAPTRTDTLPALPKSMPFEDISEQQ
                     WAAVIAANLTTAFLVCREFAPKMKARRFGRIVNFASIAARRGSSRVGVHYAAAKGGII
                     GLTKTLALELGPHNVTVNAIAPGFIKTPRIAEASWGHDSAAAEQAFISSLPLRRAGKP
                     DDIAGVVAMLCSDAGGYVSGATIDVNGGWYFGP"
     gene            complement(3354..4139)
                     /locus_tag="VGO02_05185"
     CDS             complement(3354..4139)
                     /locus_tag="VGO02_05185"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7477117.1"
                     /translation="MNRVAIVTGAAKPDSIGFAVVKGLRREGCEVVVADLYEPGFAAL
                     QDCLCVRTDVADPASVQRMVDAAMGKFNRLDILVNAVGGSWGITPQDLIASPPRGFRG
                     LTNCEIADYRTILGANLDSAFYCARAAAPHLKKQGGAIVNVASVAARKGIPPGSEGSS
                     GPYAVAKGGIMAMTRQLALELGEFGVRVNCVAPGVIASGRLKRVAELVGTNHGPEGPL
                     ATIPMGRVGTVAECAALIVALCTDDTSYMTGVTVDINGASYAA"
     gene            4175..>4753
                     /locus_tag="VGO02_05190"
     CDS             4175..>4753
                     /locus_tag="VGO02_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEV7477118.1"
                     /translation="MRQFTEDTLTDEVVARLKGVKNPRLKQLMTSLVRHLHAFAQDVK
                     LTEEEWFEAIKFLTAVGQKCDDKRQEFILLSDVLGLSMMTVAINHKTDPGATEATVLG
                     PFFAHGAKEFGYGGDLREGATMTGEDVWVSGRVTSVDGRPIPGAMLDIWQAKADGIYD
                     LQTDGEFELRGRVKANDKGEYAFKSYKPKFYSI"
BASE COUNT          796 a         1615 c         1577 g          765 t
ORIGIN      
        1 gctgctcgcc cgctaccggc agaacgtgga ttccgaccgc ggcgcgctcg ccgacctgcg
       61 cctgaacgtc ggcgtctacg gcgaccgggg cgctaccctg gtggtgttca gcgaggcgac
      121 ctggggcagc agcaaagcga acgccacctt ctacggcgtc acgccgcagc aatccgccgc
      181 ctccggcctg gccgtctatg agcccggcag cggcctcaag catctcgcgc tcggcttgat
      241 cggcggctac gacctcagcc ggcactggac gctggtggct tcggcccagc agcgctggct
      301 gcaaggcgat gtggcaaaga gtccgctggt ggaaaaaaag gggaacacgt atgcgaatgc
      361 ggggatcgct tatcgctttt agcgtcgcga gcgctaggcg ccggcgcctt tcagcagcag
      421 ctgcaccgtc tcctcgatct cggcggggtc gtcccagtcg gcctccggcg caagcagcgc
      481 gcgcgccacg atgtagccga ggttgagcga gatcaccgcg cgcgccaccg tctccggccg
      541 caggccggag cgcagctcgc cgcggcggcg gaagcgccgc atgctcgaca gcagcggctt
      601 ccacacgttg ttgcgccaca ggcggaccgt cttgcggcgc agcgcctcgt cggtcatcag
      661 cgcgccgacc agcatgcgga actgcgggcc ggcgtcgagg gccgcgtcga gccgcaccgt
      721 caggatattg cggtgccacg cggcgaacga cctggcatcg gcgccgaaca gctggccggc
      781 gtcgatcagc gcccgcggca tggcggcggc gagcagcgca gggaacatga cgcggcggaa
      841 cagcgcttcc ttgctgcgga aatacctgaa cagcgtgcgc tcggtcaccc cggccgcctc
      901 ggcgatctcg gcggtgcgcg cgccctggta gcccttctcg ctgaagatca gcgtcgccgc
      961 ctcgaggatg tcggcctcct tctgcgtcgc gggcttgcgg aaacgcgcgg tctcgcccct
     1021 gagcgcggct gctgaaacac tgcttgcgac catcggcccc cccgcgggta tagtaactgc
     1081 tatacgggta tacaggagct gccatgcgaa aagtccgcgt cgcaccgaaa ggaatgggcc
     1141 tcaacgattt cgtcgcccag caggaaggct tcttcgccgc cgagggcatc gaggtcgagt
     1201 tcgacatcaa gaccttccgc ggcacgcagt cgagctggaa gggtctggac tacaagtcgc
     1261 ggccgcagga ccggccctac gccgagggtg accagggcct gatccagtgc gcctgcgcct
     1321 ggggcagcgt gtccaacgcc tcggccggca tgggccgctt catccccgac tgctacggca
     1381 tctcgccgtg ggccatcttc gtcaggccgg actcgaagat ccgccgcccg gccgatctga
     1441 aggacgtgcc gatctcggtg ggcatgcgcg ccggcagcca cttcaacgtt ccctaccggc
     1501 tggagaagca cctgccgctc gagcgcatca aggtggtcaa caccggcggc ttcggcgcgc
     1561 gcctgcgcgc gctgctcgac ggcgaagtgg aggcggcgag cctgctgccg ccgcagatcg
     1621 acatggcgcg ccagctcggc ttgcgcatgg tgatggagga cgtgttccac accctgtggt
     1681 gggtgcccga aaccgccgac ccggccgacg tgcgcgccta cctgcgcggg ctggatcgcg
     1741 ccgaagccgc cctggcttcg gatctgaaga aatacctgcc gctatgggag aagtgcgtgc
     1801 cgcccgagtt caaggacgcg caccgctggg acttcggcgg gttcacgcgc ggcgagcgct
     1861 tcgtcttcca gccgatgccg cgctcggagt tcgacgacgt cctcggccag gtggagcgct
     1921 ggggtctgga cgagcacctg aaggagcgct ccttcgagaa gctggcgttc cgcgtctagg
     1981 ccggaggctg cgcctcgagg tccgcctcgg agtcggcccg cttcgccgcg ggctcgaaag
     2041 cgaaacacag tccgaacacc acggcgctca ggcccaggat gagccacagg atgaactcga
     2101 cgaagctctc gctcgccttg cgctcgagcc ttgacgcggt tgccagcacg tcctcgcggt
     2161 gcttgatgtc gtcctgcacc ggaggataga gcttgcgctg gccggaagcg tcgacgtact
     2221 gcgtgagctt gccggaggtg atgaaagtgc cgcgcgccgc gctgcggctc gctgcttcgc
     2281 gcttcgccgg ggacatcttc ttgccgaaat cggtgggcag ctcgtggacc gtttcggcag
     2341 gttgatagga cctcgccacg tcggcgtacg tccaatgctg ccaataggcg tagccggcca
     2401 gcatgccgac caccaccacg ccgatgccga ccaggttgct cgcacgtccc ctcggatcgt
     2461 gcgcggccag gcgccgcgtg ccttcgaaca ggcagaacag gccgaaggcc ccgcaaaccg
     2521 caatggccgt gaactgcgcc cattcgacga tcgtcatggc ggccagccta tcacggcgca
     2581 cttcgggttc aagggcataa gtcctggctc atggcccgaa gtaccagccg ccgttgacat
     2641 cgatggttgc gccggagacg tagccacccg cgtcggagca cagcatcgcc accacgccgg
     2701 cgatgtcgtc gggttttccc gcccttctca aaggcaacga agaaatgaac gcctgctccg
     2761 cggcggccga atcgtggccc cacgaagcct ctgcgatgcg cggagtcttg atgaagcccg
     2821 gcgcgatcgc gttcaccgtg acgttgtgcg ggccgagctc gagcgcgagg gtcttggtga
     2881 ggccgatgat gccgcccttc gcggcggcat agtgcacgcc cacccggctc gagccgcggc
     2941 gggcggcgat ggaagcgaag ttgacgatgc ggccgaacct gcgcgccttc atcttcggcg
     3001 cgaactcgcg gcacacgagg aatgccgtcg tcaggttggc ggcgatcacg gccgcccatt
     3061 gttgctcgct gatgtcctcg aatggcatgc tcttcggcag ggccgggagg gtatccgtcc
     3121 gtgtaggggc gatcggtccg cctgccacat tgatcaggaa gtcgatcgca ccgaccttgc
     3181 cggcgagctt ctcgacgtcg tcggcgtcgg tcaggtcggc gacgatcact ccatgcgcgt
     3241 cgtgaatgtc ggtggagaag acttcgacct gctccttctt cagcttttca accactgcgt
     3301 ggccgatccc gctggccgca ccagtgacca gcgctctctt cccctgcagt ccgctaagcg
     3361 gcatacgacg cgccgttgat gtcgaccgtg acgccggtca tgtacgaggt gtcgtcggtg
     3421 caaagcgcca cgatcagcgc ggcgcactcg gcgacggtcc cgacgcggcc catcggtatg
     3481 gtggcgagcg gcccttccgg gccatggttc gtacccacca gttcagcaac tctcttcagc
     3541 cggccgctgg cgatcacgcc cggcgcgacg cagttgaccc gcacgccgaa ctcgccgagc
     3601 tcgagcgcga gctggcgggt catcgccatg atgccgccct tcgccaccgc gtaaggcccg
     3661 ctcgagccct cggagccagg cgggatgccc ttgcgcgcgg cgaccgaggc gacgttgacg
     3721 atcgccccgc cctgcttctt cagatgcggc gcggcggcgc gagcgcagta gaacgcgctg
     3781 tccaggttgg ccccgaggat cgtgcggtag tccgcgatct cgcagttggt caggccgcgg
     3841 aagccgcgcg gcggcgacgc gatcaaatcc tgtggggtga ttccccaact gcctcccacg
     3901 gcattgacaa gaatatctaa tctattgaat ttccccatcg cggcatcgac catgcgttgc
     3961 accgacgcgg gatcggcgac gtccgtccgc acgcacaggc agtcctgcag cgccgcaaag
     4021 cccggctcgt agaggtcggc gaccaccact tcgcagccct cgcgccgcag gcccttcacc
     4081 actgcgaaac cgatgctgtc cggtttcgcc gcgccggtga cgatcgcgac ccgattcatt
     4141 tgcgttgata ttacaatgca ctggaggagg caccatgcgc cagttcaccg aagacaccct
     4201 gaccgacgaa gtcgttgcgc ggctcaaagg cgtcaagaat ccccgcttga agcagctgat
     4261 gaccagcctg gtccggcacc tgcacgcgtt cgcccaggac gtgaagctga ccgaggaaga
     4321 atggttcgag gcgatcaagt tcctcaccgc ggtcggtcag aagtgcgacg acaagcgcca
     4381 ggagttcatc ctgctctccg acgtgctcgg cctatcgatg atgacggtgg cgatcaacca
     4441 caagaccgac ccgggcgcga ccgaagccac cgtgctcggg cctttcttcg cccacggcgc
     4501 gaaggagttc ggctacggcg gcgacctgcg cgaaggggct acgatgaccg gcgaggacgt
     4561 ctgggtgagc gggcgcgtga cctcggtcga cggcaggccg atcccgggcg cgatgctcga
     4621 catctggcag gcgaaagccg acggcatcta cgacctgcag accgacggcg agttcgagct
     4681 gcgcggccgc gtcaaggcga acgacaaggg cgagtacgca ttcaagagct acaagcccaa
     4741 gttctacagc atc
//
LOCUS       DASYJA010000244         2211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2396534, whole genome shotgun sequence.
ACCESSION   DASYJA010000244 DASYJA010000000
VERSION     DASYJA010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2396534"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VGO02_05195"
     CDS             complement(<1..302)
                     /locus_tag="VGO02_05195"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEV7477119.1"
                     /translation="MNIVGLLLAAGSASRFGSDKLQHSLPHGVAIAVQAARHLKSQLP
                     RVVAVVRPGSADFSENLSREGCEVVVCENAAEGMGASLACAARAAGPADGYLVALA"
     gene            complement(299..1309)
                     /locus_tag="VGO02_05200"
     CDS             complement(299..1309)
                     /locus_tag="VGO02_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEV7477120.1"
                     /translation="MDSVDIEVLRSAEAWRKAGHRVALGTIVKTWGSAPRPVGAMVAV
                     RDDGQVTGSVSGGCVEDDLIDKVKAKLAGWTRPELFTYGVTNEEATRWGLPCGGTLQL
                     VMEPLSDTSGIQELLQKIGSQQLVRRRLEMDSGRATLEPGRWQDVLEFDGRVLSAVHG
                     PRWRLVLIGAGQLTRYLAEMAHMLDYNVTVVDPREEYAAGWDVAGVPLNRGMPDDVVR
                     ELNLDGHTALIALTHDPKLDDLALMEGLKSAAFYVGAIGSKKNNDARRVRLREFDVSQ
                     DEIARLRGPVGLYIGSKTPPEIAVAILAEMTAVRHGVSEPSWAAKPERRFDASACEVK
                     TP"
     gene            complement(1322..>2211)
                     /locus_tag="VGO02_05205"
     CDS             complement(1322..>2211)
                     /locus_tag="VGO02_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV7477121.1"
                     /translation="ILFSSLMPQMVLAVATLVIVWFGVKRGLGPLARLSEEIKERSPR
                     DLHPIDAAAAPEETRPLLAALNGLLEQVSEASRNQQRFLANAAHQLRTPLAGLQAHTE
                     LALSQPLPEAVRAQLDQVHQATIRTARLANQLLALARAEPGARGDPSHVNLKGVVESE
                     ADAWVHQALARDVDLGFELEPAPVQGDAFLLREALANLVHNAIEYSNRGGRVTVRTGA
                     RNGHAFVEVEDDGPGIPAAERGRVLERFYRVPGTSGTGSGLGLAIVREIAVGHGAGIE
                     LVEGAGAKGCRVAITFPHG"
BASE COUNT          326 a          790 c          761 g          334 t
ORIGIN      
        1 tcggcaaggg caaccaggta gccgtcggcc ggcccggcgg cgcgcgccgc acaggccagg
       61 ctcgctccca tgccctcggc ggcgttctcg cacaccacga cttcgcaacc ttccctggaa
      121 agattttccg aaaaatcggc agaaccgggc cgcacgacgg ccaccaccct gggcagctgc
      181 gacttcaggt ggcgtgcggc ctgcacggcg atggccacgc cgtgcggcag gctgtgctgc
      241 agcttgtccg agccgaaccg gcttgcagag cctgccgcaa gcagcaggcc aactatgttc
      301 aaggggtctt gacttcgcag gccgaggcgt cgaagcggcg ctccggcttc gccgcccagg
      361 acggctcgct cacgccgtgg cgcacggcgg tcatctcggc gaggatcgcc accgcgatct
      421 cgggcggggt cttggagccg atgtacaggc cgaccgggcc gcgcaggcgt gcgatctcgt
      481 cctgcgacac gtcgaactcg cgcagccgga cacggcgcgc gtcgttgttc ttcttcgagc
      541 cgatggcgcc gacgtagaac gccgccgact tgaggccctc catcagcgcg aggtcgtcga
      601 gcttgggatc gtgcgtcagc gcgatgagcg cggtgtggcc gtcgaggttg agctcgcgca
      661 ccacgtcgtc gggcatgccg cgattgagcg gcacgccggc aacgtcccag ccggccgcgt
      721 actcctcgcg cggatcgacc accgtcacgt tgtagtcgag catgtgcgcc atctcggcga
      781 ggtagcgcgt gagctggccg gcgccgatca gcaccaggcg ccagcgcggg ccgtgcaccg
      841 ccgacagcac ccgcccgtcg aactcgagca cgtcctgcca gcgccccggc tcgagcgtgg
      901 cgcggccgct gtccatctcg agcctgcggc gcaccagctg ctgggagccg attttttgca
      961 atagttcttg aattccactc gtatccgaga gcggttccat caccagttgc agggtgccgc
     1021 cgcaaggcag gccccagcgc gtggcttcct cgttggtcac gccgtaggtg aacagctccg
     1081 gcctggtcca gccggcgagc ttcgccttca ccttgtcgat cagatcgtcc tcgacgcagc
     1141 cgccggaaac cgagccggtc acctggccgt cgtcgcgcac cgccaccatc gcgccgaccg
     1201 ggcgcggcgc cgagccccag gtcttgacga tggtgcccag ggcgacgcgg tgccccgcct
     1261 tgcgccaggc ttccgctgaa cgcagtactt cgatgtcgac gctgtccata tatctaggac
     1321 actatccgtg cggaaacgtt attgcaaccc ggcagccttt cgcgcccgcg ccttcgacga
     1381 gctcgatgcc ggcgccgtgg ccgacggcga tctcgcgcac gatggcgagg cccagaccgc
     1441 tgccggtgcc cgaagtgccc ggcacgcgat agaaacgctc gaggacgcgg ccgcgctccg
     1501 ctgccgggat acccggcccg tcgtcttcga cctcgacgaa agcatggcca ttgcgcgccc
     1561 cggtgcgcac cgtgacgcgg ccaccgcggt tcgaatactc gatcgcgttg tgcaccaggt
     1621 tggcaagcgc ctcgcgcagc agaaaggcat cgccctgcac cggtgccggc tcgagctcga
     1681 agcccagatc gacgtcgcgc gccagcgcct ggtgcaccca ggcgtcggcc tcggactcga
     1741 ccacgccctt caggttgacg tgcgagggat cgccgcgcgc gccgggctcg gcgcgcgcca
     1801 gcgcgagcag ctggttggcg agccgcgcgg tgcggatggt tgcctggtgc acctggtcga
     1861 gctgcgcgcg caccgcctcg ggcagcggct gcgacagagc gagctcggtg tgcgcctgca
     1921 ggccggcaag gggcgtgcgc agctggtgcg ccgcgttcgc caagaagcgc tgctggttgc
     1981 ggctcgcctc ggagacctgc tcgagcaggc cgttcagcgc cgcgagcagc ggccgcgtct
     2041 cctccggcgc ggcggcggcg tcgatcgggt gcaggtcgcg cggtgagcgc tccttgatct
     2101 cttcggagag gcgcgcgagc gggccgaggc cgcgcttgac gccgaaccag acgatgacca
     2161 gcgtcgccac ggcgagcacc atctgcggca tcaggctgga gaacaggatc t
//
LOCUS       DASYJA010000245         3442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1670990, whole genome shotgun sequence.
ACCESSION   DASYJA010000245 DASYJA010000000
VERSION     DASYJA010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1670990"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VGO02_05210"
     CDS             <1..453
                     /locus_tag="VGO02_05210"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate binding domain-containing protein"
                     /protein_id="HEV7477122.1"
                     /translation="IVARKLGETRLVPCASPRYLAARGAPQTPEELEHHNCFTYEYET
                     PKNVWRFRDPAGGERAVRVSGSLHSNSGDLLAEAAAGGAGIVFEPAFIVGPEVRAGRL
                     VPLLQDYVPAPAPIYAVYPSRKHLSAKVRVFVDFLVARFADAQDWSSP"
     gene            450..1562
                     /locus_tag="VGO02_05215"
     CDS             450..1562
                     /locus_tag="VGO02_05215"
                     /inference="COORDINATES: protein motif:HMM:NF014324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEV7477123.1"
                     /translation="MRLLAVVVALIAGGHCFAAERGTLPDWIVASADGRVTAGQRFEL
                     VVIAPPQEEPPEELQVRLRSGADERIVTLTAVGVPSGEQRVYAGDMPANLAGPVSVEL
                     VGRSSSVLSLLVAGTADSDKLRALTGAYGEPGTEPPLSENDPMYFVVGARDGWSARFQ
                     LSFKYRLFDQASGFGKDQPWLSGLYFGYTQNSIWDLEADSKPFRDTSYRPSLFWRWER
                     TDDKTWIDGLRAGFEHESNGKEGDLSRSVNILFVRPEWRWHAHNGDSFEFTPKVYGYL
                     DKSDNPDIQRYRGYVDWRFRYDKGGEWIATPVVRVGTSGKGSLLLDVSKRVRDLKFGP
                     VSGYLQFQYFTGYGEDILDYNVRRPSQLRIGFAIVP"
     gene            complement(1559..2104)
                     /locus_tag="VGO02_05220"
     CDS             complement(1559..2104)
                     /locus_tag="VGO02_05220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7477124.1"
                     /translation="MTPRAITIRLAEPRDAQAIALMSRDFIEAGLGWKYDAPRVLRAI
                     RDRDTLAVVACEGSKSAAGRGAITGFAIMELGEERAHLVLLAVRPSHRRLGIGQRLFR
                     WLLDSARCAGMASIHLELRASNDAARRFYRAMGFYETVLVPGYYRSGEGRKEGALKML
                     RVLRVPGPVPYTWRPPRTEDT"
     gene            2201..3427
                     /locus_tag="VGO02_05225"
     CDS             2201..3427
                     /locus_tag="VGO02_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEV7477125.1"
                     /translation="MDELDRPTIQRSAADLAEEIKTRSADAAAAKLQPFGGAEIAAAL
                     LRLSPAFAQDVLAALPDETRERALAAVPDEFARQWQRNAQYEQNTVGRMMEPVLAAFA
                     PSRTVGETIESLREMVKSAFITYVYVLDPDEHLLGIVTMRDLLFSENGRTLGEVMIKD
                     VFALHAAARLQDGMKQVLDRHYPVYPVVDGERHLIGLVRGQAMFEAQAIEISLQAGTM
                     MGVEKEERIGTPWFDSLKMRHPWLQLNLLTAFLAAAVVGMFQDTIDRLVILALFLPVL
                     AGQSGNTGCQALAVTLRGMTLGELKPGMERRLVTKEALLGLLNGAGVGVAAGVGMYLT
                     ATGQHSGFPWMLSFVVFLAMIGACVASGISGAMVPLTLKRFGFDPATASSIFLTTATD
                     VVSMGMLLGLATLLVR"
BASE COUNT          518 a         1164 c         1208 g          552 t
ORIGIN      
        1 atcgtcgcgc gcaagctcgg cgaaacccgc ctcgtgccct gcgcctcgcc gcgctacctc
       61 gcggcgcgcg gcgcgccgca gacgcccgag gagctcgagc accacaactg cttcacctac
      121 gagtacgaga cgccgaagaa cgtctggcgc tttcgcgatc cggcgggcgg cgagcgtgcg
      181 gtgcgggtga gcggcagcct gcacagcaac agcggcgatc tgctggccga agcggcggcg
      241 ggcggcgccg gcatcgtgtt cgagcccgcc ttcatcgtcg gtcccgaggt gcgcgccggg
      301 cgcctggtgc cgctcctgca ggattacgtg ccggcgccgg cgccgatcta cgcggtctat
      361 ccgagtcgca agcacctctc ggcgaaggtg cgcgtgttcg tcgatttcct ggtcgcgcgc
      421 ttcgccgacg cgcaggactg gagctcgcca tgaggctgct ggcagtcgtt gtcgcgctca
      481 tcgccggcgg ccattgcttt gccgccgagc gcggcaccct tccggactgg atcgtggcct
      541 ccgccgacgg gcgcgtcacc gccgggcagc gcttcgagct ggtcgtgatc gcaccgccgc
      601 aggaagagcc gccggaggag ctgcaggtgc ggctcaggtc gggcgccgac gagcgcatcg
      661 tcacgctgac ggcggtcggc gtgccgagcg gcgagcagcg cgtgtacgcg ggcgacatgc
      721 cggcgaatct cgccgggccg gtgtcggtgg aactggtcgg gcgctcctcg agcgtgctgt
      781 cgctgctggt ggccggcacg gccgactccg acaagctgcg cgcgctcacc ggcgcttacg
      841 gcgagccggg caccgagccg ccgctgtcgg agaacgatcc gatgtatttc gtggtcggcg
      901 cgcgcgacgg ctggagcgcc cgcttccagc tctcgttcaa gtaccgcctg ttcgaccagg
      961 cttcgggatt cggcaaggac cagccgtggc tctcgggcct gtacttcggc tacacgcaga
     1021 actcgatctg ggacctggag gcggattcca agccgttccg cgacaccagc taccggccct
     1081 cgctcttctg gcgctgggag cgcaccgacg acaagacctg gatcgacggc ctgcgcgccg
     1141 gcttcgagca cgagtcgaac ggcaaggagg gcgacctgtc gcgctcggtc aacatcctct
     1201 tcgtgcggcc ggaatggcgc tggcatgcgc acaacggcga cagcttcgag ttcaccccga
     1261 aggtctatgg ctacctcgac aagtcggaca acccggatat ccagcgctac cgcgggtatg
     1321 tcgactggcg ctttcgctac gacaagggcg gcgagtggat cgcgacgccg gtggtgcgcg
     1381 tcggcacttc gggcaagggc agcctgctgc tggacgtttc caagcgcgtg cgcgacctga
     1441 agttcggacc ggtcagcggc tatctgcagt tccagtattt caccggctac ggcgaggaca
     1501 ttctcgacta caacgtgcgc cggccctcgc agctcaggat cggcttcgcc atagttccct
     1561 aggtgtcttc ggtgcgcggc gggcgccagg tgtagggcac cgggcccgga acgcgcagca
     1621 cgcgcagcat cttgagcgcg ccttccttgc ggccctcgcc gctgcggtag tagcccggca
     1681 ccagcacggt ctcgtagaag cccatcgcgc ggtagaagcg ccgcgcggca tcgttgctcg
     1741 cgcgcagctc gagatggatg ctcgccatgc cggcgcagcg tgccgagtcg agcagccagc
     1801 ggaacagccg ctgcccgatg ccgaggcggc ggtgagaagg gcgcacggcg agcagcacga
     1861 ggtgcgcgcg ctcctcgccg agctccatga tcgcgaagcc ggtgatcgcg ccgcgcccgg
     1921 cggcgctctt gctgccttcg caggccacca cggcgagcgt gtcgcggtcg cggatggcgc
     1981 gcagcacccg cggcgcgtcg tatttccagc ccaggccggc ctcgatgaag tcgcgcgaca
     2041 tcagcgcgat cgcctgcgcg tcgcgcggct cggcgaggcg gatggtgatg gcgcgcggcg
     2101 tcatgagcag aattgtgcca tgacggcgcg ccgtcaggat gccctgagga gactagggta
     2161 ttctgcgatt tcgggaagct gaaacaaaag gggagacccg atggacgaac tagaccgtcc
     2221 caccatccag cgctccgcgg ccgaccttgc cgaggaaatc aagacccgct ccgccgacgc
     2281 cgcggccgcg aagctgcagc ctttcggcgg cgctgaaatc gccgccgcgc tgctgcggct
     2341 gtcgcccgcc ttcgcgcagg acgtgctcgc cgcgctgccc gacgagacac gcgagcgcgc
     2401 gctcgccgcc gtgcccgatg agttcgcccg ccaatggcag cgcaacgcgc agtacgaaca
     2461 gaatacggtc gggcggatga tggagccggt gctggcggcg tttgcgccct cgcgcacggt
     2521 cggcgagacc atcgagtcgc tgcgcgagat ggtgaagagc gcgttcatca cctacgtcta
     2581 cgtgctcgac ccggacgagc acctgctcgg catcgtcacc atgcgcgacc tgctgttcag
     2641 cgagaacggc aggacgctcg gcgaggtgat gatcaaggac gtgttcgcgc tgcacgccgc
     2701 ggcgcgcctg caggacggga tgaagcaggt gctcgaccgc cactatccgg tgtatccggt
     2761 agtcgacggc gagcggcacc tgatcggcct ggtgcgcggc caggcgatgt tcgaggcgca
     2821 ggcgatcgag atctcgctgc aggccggcac gatgatgggc gtggagaagg aggagcgcat
     2881 cggcacgccc tggttcgaca gcctcaagat gcgccatccg tggctgcagc tcaacctgct
     2941 gaccgcgttt ctcgccgcgg cggtagtggg aatgttccag gacaccatcg accgcctggt
     3001 gatcctcgcc cttttcctgc cggtgctcgc cgggcagtcg ggcaacaccg gctgccaggc
     3061 gctcgccgtg accctgcgcg gcatgacgct gggcgagctc aagccgggaa tggagcgccg
     3121 gctggtaacg aaggaagcgc tgctcggcct gctgaacggc gccggcgtcg gcgttgccgc
     3181 cggcgtcggc atgtacctca ccgccaccgg gcagcattcg ggttttccct ggatgctgtc
     3241 cttcgtcgtg ttcctcgcca tgatcggcgc ctgcgtggcg agcggcatct ccggggcgat
     3301 ggtgccgctt acgctgaagc gcttcggctt cgacccggcg accgcctcga gcatcttcct
     3361 caccaccgcc accgatgtgg tgagcatggg aatgctgctg gggctggcca cgcttctggt
     3421 gcgttagaca tagatcatct tg
//
LOCUS       DASYJA010000246         1905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_764293, whole genome shotgun sequence.
ACCESSION   DASYJA010000246 DASYJA010000000
VERSION     DASYJA010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764293"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="VGO02_05230"
     CDS             complement(<1..1284)
                     /locus_tag="VGO02_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEV7477126.1"
                     /translation="MKKLKEVDVVIVGLGWTGGILAKELSETGLKVVALERGAMRTTA
                     TDFSVPHVRDELRFVVRHDLMQNTARDTLTIRNNSSQEALPMRRLGSFLPGEVVGGSA
                     VHWSGHTWRWTDMEFNIRSEYERRYGKKFIPADMTIQDWGITYAELEPYYDKFERTAA
                     ISGKAGNLQGRIVPGGNPFEAPREREYPLPPLTPILASEMFTEAARNQGYHPFPRPSA
                     NASRAYTNPDGSRFGACQYCGFCQRFGCEANAKGSPHITVIPIAMRQPSFELRTHAWV
                     TKVLKDPKAKRVTGVTYTSVLTGEEFEQPAQLVLLCAYAINNVHLMLLSGIGQPYDPA
                     SQKGVVGKNYCYQTGASATLFFEGKYFNPFINAGGSNATIDDFNINWDFDRGPQGFIG
                     GYNVAGGFNTPLPIGYRPVPSGTPRWGKAWKAATAK"
     gene            complement(1281..>1905)
                     /locus_tag="VGO02_05235"
     CDS             complement(1281..>1905)
                     /locus_tag="VGO02_05235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEV7477127.1"
                     /translation="PQSAQAQAPAAAAPAASGYEFLNVEEAAFVEALVDHMVPADELT
                     PKGTDIGINVFIDRALAGGWGKGDRLYMQGPWKEGTPSQGYQLPLTPADLYRAGIAAA
                     NAVCAKTYDKPFHQLEEAKREEFLLALSGGKVSFENGPPARVFFGMLYQNVMEGMFSD
                     PLYGGNRDKAGWKLIGFPGVIAVHYSDVEKFRGKKYEVKPVSIQDMS"
BASE COUNT          283 a          615 c          651 g          356 t
ORIGIN      
        1 cttcgcggtc gctgccttcc acgctttgcc ccagcgcggc gtgcccgagg gcaccggccg
       61 gtagccgatc ggcagcggcg tgttgaatcc gcccgccacg ttgtagccgc cgatgaagcc
      121 ctgcgggccg cggtcgaaat cccagttgat gttgaagtcg tcgatggtgg cgttcgagcc
      181 gccggcgttg atgaagggat tgaagtactt gccctcgaag aagagcgtcg cgctggcacc
      241 ggtctggtag cagtagttct tgccgaccac gcccttctgc gacgcggggt cgtaaggctg
      301 gccgatgccg gagagcagca tgaggtgaac gttgttgatc gcgtaggcgc aaagcagcac
      361 gagctgcgcg ggctgctcga actcttcgcc ggtgaggacg ctggtgtagg tcacgccggt
      421 tacgcgcttg gccttcggat ccttcagcac cttcgttacc caggcgtgcg tgcgcagctc
      481 gaagctcggc tggcgcatcg cgatcgggat caccgtgatg tgcgggctgc ccttggcgtt
      541 cgcctcgcag ccgaagcgct ggcagaagcc gcagtactgg caggcgccga agcgcgagcc
      601 gtccgggttg gtgtaggcgc gcgaggcatt ggccgagggc cgcgggaacg ggtggtagcc
      661 ctgattcctc gccgcctcgg tgaacatctc gctcgcgaga atcggcgtga gcggcggcag
      721 cgggtactcg cgctcgcgcg gcgcctcgaa cgggttgccg cccggcacga tcctgccctg
      781 caggttgccg gccttgccgg agatggcggc ggtgcgctcg aacttgtcgt agtagggctc
      841 gagctcggcg taggtgatgc cccagtcctg gatcgtcatg tcggccggga tgaatttctt
      901 gccgtagcgg cgctcgtact cgctgcggat gttgaactcc atgtcggtcc agcgccaggt
      961 atggccgctc cagtgcaccg ccgagccgcc caccacctcg cctggcagga acgagccgag
     1021 gcggcgcatc ggcagcgctt cctgcgagga gttgttgcgg atggtgagcg tatcgcgcgc
     1081 ggtgttctgc atgaggtcgt ggcgcaccac gaacctcagc tcgtcgcgca cgtgcggcac
     1141 cgagaaatcg gtggcggtgg tgcgcatcgc gccgcgctcg agcgcgacca ccttgaggcc
     1201 ggtctcggag agttctttcg ccagaatgcc gcccgtccac ccgaggccga cgataacgac
     1261 atcgacttcc ttgagctttt tcatgacatg tcctggatgc tgacgggctt cacctcgtac
     1321 ttcttgccgc ggaatttctc gacgtccgag tagtgcaccg cgatcacgcc ggggaagccg
     1381 atcagcttcc agccggcctt gtcgcggttg ccgccataga gcgggtcgga gaacatgccc
     1441 tccatcacgt tctgatacag catgccaaaa aacacgcgcg ccggcggccc gttctcgaag
     1501 ctcacctttc cgcccgacag ggcgagcagg aattcctcgc gcttcgcctc ctcgagctgg
     1561 tggaacggct tgtcgtaggt cttcgcgcag acggcgttcg ccgcggcgat gccggcgcga
     1621 tacaggtccg ccggggtcag cggcagctgg tagccctggc tcggcgtgcc ttccttccag
     1681 ggaccctgca tgtacaaccg gtcgcccttg ccccagcctc cggcgagcgc ccggtcgatg
     1741 aacacgttga tgccgatgtc ggtacctttc ggcgtgagct catcggccgg caccatgtgg
     1801 tcgaccagcg cctcgacgaa ggcagcctcc tcgacgttga ggaattcgta gccggaagcg
     1861 gccggcgctg cggcggcagg cgcttgcgcc tgcgcgcttt gcggc
//
LOCUS       DASYJA010000247         3307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3034168, whole genome shotgun sequence.
ACCESSION   DASYJA010000247 DASYJA010000000
VERSION     DASYJA010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3034168"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VGO02_05240"
     CDS             <1..630
                     /locus_tag="VGO02_05240"
                     /inference="COORDINATES: protein motif:HMM:NF018652.4"
                     /note="catalyzes the oxygen-independent formation of
                     protoporphyrinogen-IX from coproporphyrinogen-III; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HEV7477128.1"
                     /translation="PGQSIAMARADIEAGVATGVPHVSAYQLTIEPNTVFFSKPPKLP
                     EHDLAADMQVMIEELLKQNEFQHYETSAFAKPGHRCRHNLNYWEFGDYLGIGAGAHGK
                     VSFPDRVTRHSRIKQPREYLAAQNTLAEEKTIPAAELSFEFMLNALRLQDGFSVSLLT
                     ERIGLAVSVIEHQLQQAEKQGLIERDWKRIRPSARGQRFLNELLELFLR"
     gene            complement(627..1238)
                     /locus_tag="VGO02_05245"
     CDS             complement(627..1238)
                     /locus_tag="VGO02_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1989 domain-containing protein"
                     /protein_id="HEV7477129.1"
                     /translation="MQIPGLKLLDEVIPARAGWSRELSRGQVLRLVDLEGRQAVDFLC
                     YNALDYEDRYAAADTMKINGNIFIRKGTVLYSVNCNPMFTVIEDTCGCHDTIGGCCSS
                     ALNRRRYGKPDDPSCRQNFLAQLGRYGMGPRDMAANLNFFMYVPVGKDGAMDMGPSIS
                     KPGDYLDLRAEMDVLAVISNCPQMNNPVNDYNPTPVRAIVYQP"
     gene            complement(1255..1692)
                     /locus_tag="VGO02_05250"
     CDS             complement(1255..1692)
                     /locus_tag="VGO02_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477130.1"
                     /translation="MKIIVFILVLAAAGYWGYRHGYIKPEWFGGGTAATPGNAPAESL
                     EQTLNRGLPRMVSNDISADRAVASNQVVTFNYRFVDLDQYAVAQRYGATLPAEMQGTL
                     IRDLCGNRAVREQVLARGRDVVLQIRAQDGRTMFTTQLRPGGC"
     gene            complement(1704..2345)
                     /gene="trmB"
                     /locus_tag="VGO02_05255"
     CDS             complement(1704..2345)
                     /gene="trmB"
                     /locus_tag="VGO02_05255"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEV7477131.1"
                     /translation="MNRPIRSFVLRQGRTTPAQGRALEQLYPKYGVAFSDQKISSPRN
                     APLIVEIGSGMGETTIEIAKAHPQADFIAIEVHGPGVGSLLNRIDAEGLNNLRVIRHD
                     AVEVLERMVPDGSLAAIHLFFPDPWPKKRHHKRRLVQPAFASLVARKLRGGGIVHAAT
                     DWPEYADQIARVFSQDEAFAHSASGFAARPMTKFEKRGRRLGNPVRDLVFVRR"
     gene            complement(2342..3142)
                     /locus_tag="VGO02_05260"
     CDS             complement(2342..3142)
                     /locus_tag="VGO02_05260"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEV7477132.1"
                     /translation="MTAQDRLVVAGRAYDSRLLVGTGKYRDFAETRAAVEASGAEIVT
                     VAIRRTNIGQNANEPSLLEALPPSRFTYLPNTAGCYSAEDAVRTLRLARELLDGHELV
                     KLEVLGDPQTLYPNMPETLKAAEQLVKEGFKVMVYCSDDPIQAQMLEGIGCVAVMPLA
                     SLIGSGMGILNPWSLQLVLEKVKVPVLVDAGVGTASDAAIAMELGCDGVLMNTAIAAA
                     QNPVMMARAMKKAVEAGREAFLAGRMPKKLYAATPSSPTEGKISSKAA"
     gene            complement(3145..3306)
                     /gene="thiS"
                     /locus_tag="VGO02_05265"
     CDS             complement(3145..3306)
                     /gene="thiS"
                     /locus_tag="VGO02_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEV7477133.1"
                     /translation="MALTFSQLLDQLALAGRRLAVERNGQIVPRSCFGDALLADGDRI
                     EVVMAVGGG"
BASE COUNT          538 a         1077 c         1116 g          576 t
ORIGIN      
        1 ccgggccaga gcatcgccat ggcgcgcgcc gacatcgagg ccggcgtggc gacgggcgtg
       61 ccgcatgttt ccgcctatca gctcaccatc gagccgaata ccgttttctt cagcaagccg
      121 ccgaagctgc cggagcacga tctcgccgcc gacatgcagg tgatgattga agaattgttg
      181 aagcaaaatg aatttcagca ctacgagaca tcggcattcg ccaagcccgg ccaccgttgc
      241 cggcacaacc tcaactattg ggagttcggc gactacctcg gcatcggcgc cggcgcgcac
      301 ggcaaggtca gcttccccga ccgcgtgacg cgtcactcgc gcatcaagca gccgcgcgaa
      361 tacctcgctg cgcagaacac gctggcggag gagaagacga tccccgccgc cgagctgtcg
      421 tttgagttca tgctgaatgc ccttcgcctg caagatggct tttcagtcag tctcctgact
      481 gaaagaatcg ggctggcggt gagcgtgatc gagcatcaac tgcagcaggc ggaaaagcag
      541 ggactgatcg agcgcgactg gaagcgcatt cggccgagcg cgagaggaca gcgcttcctc
      601 aacgagctgc tggagctgtt cctgcgctag ggctggtaga cgatggcgcg caccggcgtc
      661 gggttgtagt cgttcaccgg gttgttcatc tgcgggcaat tggaaatcac cgcgagcacg
      721 tccatctcgg cgcgcaggtc gaggtagtcg ccgggcttgg agatgctcgg gcccatgtcc
      781 atcgcgccgt ccttgccgac cggcacgtac atgaagaagt tcaggttggc cgccatgtcg
      841 cgcggcccca tgccgtagcg gccgagctgg gcgaggaagt tctgccggca gctcgggtcg
      901 tcgggcttgc cgtagcgccg gcggttgagc gccgacgagc agcagccgcc gatggtgtcg
      961 tggcagccgc aggtgtcctc gatcaccgtg aacatcgggt tgcagttcac cgagtagagc
     1021 accgtgccct tgcggatgaa gatgttgccg ttgatcttca tcgtgtctgc cgcggcgtag
     1081 cggtcctcgt agtcgagcgc gttgtagcag aggaaatcga ccgcctggcg gccctcgagg
     1141 tcgaccaggc gcagcacctg gccgcgcgac agctcgcggc tccatccggc gcgcgccggg
     1201 atgacttcgt cgaggagctt caagccggga atctgcatgg cgcgattcta ttcttcagca
     1261 gccgccgggc cgcagttggg tggtgaacat cgtccggccg tcctgcgcgc ggatctgcag
     1321 cacgacgtcg cgtccgcgcg ccaggacctg ctcgcgcacc gcgcgattgc cgcacaggtc
     1381 gcggatgagc gtgccctgca tctcggcggg cagggtggcg ccgtagcgct gcgccaccgc
     1441 gtactggtcg agatcgacga agcggtagtt gaaggtcacc acttggttgc tcgcgacggc
     1501 ccggtccgcg ctgatgtcat tgctcaccat tcgcggcagg ccgcggttga gggtctgttc
     1561 gagcgattcg gcgggcgcgt tgccgggggt ggccgccgtg cctccgccga accactcggg
     1621 cttgatatag ccgtggcgat acccccagta cccggcggcg gcgaggacga ggatgaagac
     1681 gatgattttc aaggggacgc tccttacctg cgaacgaaca ccaggtcgcg caccggattg
     1741 cccaggcggc ggcctcgctt ctcgaacttg gtcatcggcc gcgccgcgaa tccgctcgcg
     1801 ctgtgcgcaa aggcttcgtc ttgcgaaaag acccgtgcga tctggtcggc gtactccggc
     1861 cagtcggtgg cggcgtgcac gatgccgccg ccccgcaatt tcctcgcgac gagggaggcg
     1921 aacgccggct gcaccaggcg gcgcttgtgg tggcgcttct tcggccaggg gtcgggaaag
     1981 aaaaggtgga tggcggcgag cgagccgtcc ggcaccatgc gctcgagcac ctcgaccgcg
     2041 tcgtgccgga tgacgcgaag gttgttcaat ccctctgcat cgatccggtt caacaggctg
     2101 ccgacgccgg gcccgtgcac ctcgatggcg atgaaatcgg cttgcggatg cgccttggcg
     2161 atctcgatcg tggtttcgcc catcccggag ccgatctcga cgatcagcgg cgcgttgcgg
     2221 ggggaagaga tcttctggtc ggaaaaagcg acgccgtact tcggatagag ctgctccagc
     2281 gcgcggccct gggccggtgt ggttctgcct tgtcgcagaa caaagctgcg gataggccga
     2341 ttcaagcggc cttcgatgaa atctttcctt cggtgggaga gctgggggtt gccgcgtaga
     2401 gcttctttgg catgcggcca gcgaggaagg cctcgcggcc ggcctcgacc gcctttttca
     2461 tcgcccgcgc catcatcacc gggttctgcg ccgcagcgat ggcggtgttc atcaggacgc
     2521 cgtcgcagcc cagctccatc gcgatcgcgg cgtcggaggc ggtgcccacg ccggcgtcga
     2581 ccagcaccgg caccttcacc ttctccagca ctagctggag gctccacgga ttcaggatgc
     2641 ccatgccgga gccgatgagc gaggcgagcg gcatcaccgc gacgcagccg atgccctcca
     2701 gcatctgcgc ctggatcggg tcgtcggagc agtacaccat caccttgaag ccttccttca
     2761 ccagctgctc ggccgccttc agcgtctcgg gcatgttggg gtagagcgtc tgcgggtcgc
     2821 ccagcacctc cagcttgacc agctcgtggc cgtcgagcag ctcgcgcgcg aggcgcagtg
     2881 tgcgcaccgc gtcctcggcg gagtagcagc cggccgtatt cggcagatag gtgaacctcg
     2941 agggcggcag cgcctcgagc aggctcggct cgttcgcgtt ctggccgatg ttggtgcggc
     3001 ggatcgccac cgtcacgatc tcggcgcccg aggcttccac cgcggcgcgc gtctcggcga
     3061 agtcgcggta cttgccggtg ccgaccagca gccgcgagtc gtacgctcgg ccggccacca
     3121 cgagcctgtc ctgcgccgtc atcgtcatcc tccgcccacc gccatcacga cctcgatgcg
     3181 atcgccgtcg gccagcagcg catcgccgaa acaactgcgc ggcacgatct ggccgttgcg
     3241 ctcgaccgcg agccgccgcc cggcgagggc gagctggtcg aggagctggg aaaaggtgag
     3301 cgccacg
//
LOCUS       DASYJA010000248         3833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1127594, whole genome shotgun sequence.
ACCESSION   DASYJA010000248 DASYJA010000000
VERSION     DASYJA010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127594"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VGO02_05270"
     CDS             complement(<1..701)
                     /locus_tag="VGO02_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7477134.1"
                     /translation="MRVADSDILNAGILIVDDQEVNVSLLAQMLQDAGYTSVASTMDP
                     HQVCELHRKNHYDLILLDLQMPGMDGFQVMEALKTNDADGYLPVLVITAQPGHKLRAL
                     QAGAKDFVAKPFDLVEVRTRIRNMLEVRLLYKKLEGYSKGLEHTVRERTAELRESEAR
                     YRSLTELASNWYWEQDERGNFTKVSGPVLEMLGIRVSDLAGTTDSGTGHGWNETERAA
                     LQATIEARQPFLDFHL"
     gene            complement(698..2338)
                     /locus_tag="VGO02_05275"
     CDS             complement(698..2338)
                     /locus_tag="VGO02_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV7477135.1"
                     /translation="MSKSDHPTQQALLKAGALQNAILTSANFSIIATDEKGIIQLFNV
                     GAERMLGYTAAEVVNRISPSDIHDPREVTARAEALSLELSTAIAPGFEALAFKASRGI
                     ADSYELTYIRKDGGRFPAVVSITALRDDYGDVIGYLLIGADNTVRKRAESALNEAMAA
                     AEKANLAKSDFLSGMSHELRTPLNAILGFAQLVDTGTPAPTPAQKRNIDQILKAGWYL
                     LELINEILDLSLIESGKLTLSAEPVSLVEVMLECRAMIEPQAQKRGISMRFPHFEIPY
                     FVKADRTRVKQVLINLLFNAIKYNKQAGAVAVEYSLRPPHSIRISVRDTGAGLAAEQL
                     SQLFQPFNRLGKEASAEEGTGIGLVMTKRLIELMGGTIGADSTVGVGSVFWFELGLTS
                     APQLAVFEAEQAVLARPQSPDGAALRTLLYVEDNPANLELVEQLIARRSDLRLLSAAD
                     GNVGVEYARAYLPDVILMDINLPGISGVEALRILRADPSTAHIPVIALSANAIPRDID
                     RGLEAGFFTYLTKPIKVNQFMEALDVALKFSQTEKEQA"
     gene            complement(2411..3493)
                     /locus_tag="VGO02_05280"
     CDS             complement(2411..3493)
                     /locus_tag="VGO02_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV7477136.1"
                     /translation="MQLAVGWLALVALVAALYLARGFFVPLLIGILASYALHPMVNWL
                     EAWRVPRFIGAALVLAMVVGSFSWIGVSMSGDAEALIEKLPEAARKLREDLSDARSGA
                     PTALQNMQQAAKELEGAAADAGAKPKARLVAAQVTEPAWLREPTWLRDYTLAQSALLV
                     AVAAQTPIVLLLTYFLLASGQHFRRKLLQLVGPSLARKKDAVEILEQVDVQVQRYLLA
                     MFLSNVLLAAGTWLAFLALGLDQAGVWGVAAGVLHFIPYLGAVLIALGSGVAAFLQFG
                     SLLHAVAVAGVSLLVASAVGLAFMTWLHGRFARVNAAVLFIGLLFFGWLWGVWGLLLG
                     APLVAIAKVIFDRVESLKPAGALLER"
     gene            complement(3518..3667)
                     /locus_tag="VGO02_05285"
     CDS             complement(3518..3667)
                     /locus_tag="VGO02_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEV7477137.1"
                     /translation="MLYTIAVVLIVLWLLGLVSSYTVGGFIHVLLVIAVVMILLRIIS
                     GRRLV"
BASE COUNT          667 a         1253 c         1257 g          656 t
ORIGIN      
        1 aggtggaagt ccaggaacgg ctggcgcgcc tcgatcgtcg cctgcagcgc tgcacgctct
       61 gtttcattcc atccatgacc agtgccgctg tctgtggttc ccgccaggtc ggacacgcgt
      121 atgcccagca tttcaagcac ggggccggaa actttggtga agttcccgcg ctcgtcctgc
      181 tcccagtacc agttcgaagc caactcggtc aggctgcgat agcgggcttc gctttcgcgc
      241 agctcggcgg tccgctcgcg caccgtatgc tccaggccct tgctgtagcc ctcgagcttc
      301 ttgtagagca ggcgcacttc gagcatgttg cggatgcgcg tcctgacttc gaccaggtcg
      361 aacggcttgg ctacgaaatc ctttgcgccg gcctgcaacg cgcgcagctt gtggccgggc
      421 tgggcggtga tcacgaggac cggcaggtag ccgtccgcat cgttggtctt gagggcctcc
      481 atcacctgga agccgtccat gccgggcatc tgcaggtcga gcaggatcag gtcgtaatgg
      541 ttcttgcgat gcagctcgca gacctggtgc gggtccatgg tcgaggcgac cgaggtgtag
      601 ccggcgtcct gcagcatttg cgccagcagg ctgacattga cctcctggtc gtcgacgatg
      661 aggatgccgg cgttgagaat gtcggaatcg gcaaccctca tgcctgttcc ttttcggttt
      721 gcgagaattt cagtgccacg tcgagcgcct ccatgaactg gttgaccttg atgggtttgg
      781 tcagataggt gaagaatccg gcctcgaggc cccggtcgat gtcgcgcggt atcgcgttgg
      841 cgctgagcgc gatgacgggg atatgcgccg tcgacggatc cgcgcgcagg attctcagcg
      901 cttcgacccc gctgatgccg ggcaggttga tgtccatcag gatcacgtcg ggcaggtagg
      961 cgcgcgcata ttcgacgccc acattgccgt ccgctgcgct cagcaggcgc aggtcggaac
     1021 gccgcgcaat cagctgctcg accagctcca ggtttgccgg attgtcttcc acatagagca
     1081 gggtgcgcag cgcggcgcca tcgggcgact gcggccgcgc cagcaccgct tgctcggctt
     1141 cgaaaaccgc cagctgcggc gcgctcgtca ggcccagctc gaaccagaac acgctgccca
     1201 ccccgacggt gctgtccgcg ccgatcgtgc cgcccatcag ctcgatcagc cgcttggtca
     1261 tcaccaggcc gatgcccgtg ccctcctctg cgctggcctc cttgccgagc cggttgaacg
     1321 gctggaagag ctgcgaaagc tgctccgcgg ccaggcccgc gccggtgtcc cgcacgctga
     1381 tgcggatcga atgcgggggc cgcagggagt actccacggc aaccgcgccg gcctgcttgt
     1441 tgtacttgat cgcgttgaat agaagattga tcaggacctg cttgacccgg gtgcggtcgg
     1501 ccttgacgaa gtagggaatc tcgaagtgcg gaaaccgcat gctgatgccg cgtttctgcg
     1561 cctgcggctc gatcatggcg cggcactcga gcatcacctc gaccagcgag accggctcgg
     1621 ccgacaaggt cagcttgccc gactcgatca gcgacaggtc gaggatctcg ttgatcagct
     1681 cgagcaggta ccaccccgcc ttcaggatct ggtcgatgtt gcgcttctgc gcgggcgtcg
     1741 gcgccggcgt gcccgtatcc accagctggg cgaagccgag gatcgcgttc agcggcgtgc
     1801 gcaattcatg gctcatgccg gagagaaagt ccgatttcgc gagattcgcc ttctcggcgg
     1861 ccgccatggc ctcgttgagg gcggattcgg cgcgcttgcg caccgtattg tcggcgccga
     1921 tcagcaggta gccgatgaca tcgccatagt cgtcgcgcag cgccgtgatc gacaccacgg
     1981 ccggaaagcg gccgccgtcc ttgcggatat aggtcagctc atagctgtcc gcgatgccgc
     2041 gcgaggcctt gaaagccagc gcctcgaagc ccggcgcgat ggcggtcgac agctcgaggc
     2101 tcaatgcctc ggctcgcgcc gtgacctcgc gcggatcgtg aatatcgctc gggctgatcc
     2161 ggttgaccac ctcggccgcc gtatagccaa gcatgcgctc ggcgccgacg ttgaacagct
     2221 ggatgatgcc cttctcgtcg gtggcgatga tcgagaaatt ggcgctggtc aggatcgcgt
     2281 tctgcagggc ccccgccttg agcaaggcct gctgggtggg atggtcggat ttggacatgg
     2341 agacggccac aatcgcggtc tttgcgtcta gcgtctgtgc ggtggggaac agagcggctg
     2401 gcgcccgagc ctatcgttcg agaagcgcgc ccgcgggctt gagcgattcg acccggtcga
     2461 agatcacctt ggcgatggcc accaggggcg cgccgagcag cagtccccag acgccccaca
     2521 gccagccgaa aaacagcagc ccgatgaaca gcaccgcggc attcacgcgc gcgaaccggc
     2581 cgtgcagcca ggtcatgaat gccagcccca cagcgcttgc aaccagcagc gatacgcccg
     2641 ccaccgcaac ggcatgcagc agcgacccga actgcaggaa cgcggcaacg ccgctgccga
     2701 gcgcgatcag caccgcgccg aggtagggga tgaaatgaag gacgccggcg gcgacgcccc
     2761 agacgcccgc ctggtccagg ccgagcgcca gaaatgcgag ccaggtgccg gcggccagca
     2821 ggacgttcga caggaacatg gcgagcaggt agcgctgcac ctgcacgtcg acttgctcca
     2881 ggatctccac ggcgtccttc ttgcgcgcca gcgacggccc gacgagctgc agcagcttgc
     2941 gccggaagtg ctggccggaa gcgagcagga aataggtcag cagcagcacg atcggagtct
     3001 gcgcggcgac cgcgaccagc agcgcggact gcgccagcgt gtaatcgcgc agccaggtgg
     3061 gctcgcgcag ccaagcgggc tcggtcacct gggccgcgac caagcgtgct ttcggcttgg
     3121 cgcctgcgtc ggcggcggcc ccctccagct ccttggcggc ttgctgcatg ttctgcagcg
     3181 cggtcggggc gcccgagcgg gcgtcgctca ggtcctcgcg cagcttgcgg gcggcctcgg
     3241 gcagcttctc gatcagcgct tcggcgtcgc cgctcatcga gacgccgatc caggagaagc
     3301 tccccaccac catcgccagt accagcgccg cgccgatgaa gcgcggcacg cgccaggcct
     3361 cgagccagtt gaccatcggg tgcagcgcgt agctcgcgag gatgccgatc agcagcggaa
     3421 cgaagaagcc gcgcgccagg tacagggcgg cgaccagcgc cacgagcgcc agccagccca
     3481 ccgccagttg catgcgcgag ttaacgggga ctatccgtca gaccagtctt cggccgctga
     3541 tgatcctgag caggatcatc accaccgcga tcaccagcag gacgtgaatg aacccgccga
     3601 cggtgtagga ggagacgagc cccaggagcc acaggacaat gagtacaact gcaatcgtat
     3661 aaagcatgtc gatccccttt cgaaaaaaag ccccgctcgc gcggggcttc gaatcaatcc
     3721 cgcgttactg cttcgcgatc ctggttccgg acacttcgat gaagacgtgg cggtcaggct
     3781 gcatgcaggc aacgttcttg gcgttgttgg cgtccttgca ttcgcccgcg tag
//
LOCUS       DASYJA010000249         5749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_764799, whole genome shotgun sequence.
ACCESSION   DASYJA010000249 DASYJA010000000
VERSION     DASYJA010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764799"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VGO02_05290"
     CDS             complement(<1..942)
                     /locus_tag="VGO02_05290"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV7477138.1"
                     /translation="MKGRLEDHRMLTGQGRYVSDWNLPGQAYGCFLRSDRAHAKIVSI
                     QTEDALKSPGVIAVFTGQELVDAGLKPIPAAAPFKWRDGSAQRLAQRPSLACEVVRHV
                     GEPIALIVAGTAAQAQDAAEAVVVEYADLPAAVTAAQAMAAGAPQLHPGAPGNLVLDF
                     AGGDEAATNAAFAAAAKVVKLTDYHSRVIGNPMEPRTALGSYDSSRDLYFLHGTTQGT
                     GPMRGQLSAVLGVPPEKVRVVAEEVGGGFGVRFNAYPEYGALLYAAKKLGRPVKWVGT
                     RSEVFLGDEQARDIVHTAEMALDANGRILGMRFDYLSN"
     gene            complement(1004..1663)
                     /locus_tag="VGO02_05295"
     CDS             complement(1004..1663)
                     /locus_tag="VGO02_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477139.1"
                     /translation="MKKIIRSLGLAAVLAGASAFAAEDVGLVNQLSGDVSYTSGSNTA
                     KAKAFMKMREGDRFSIPAGAQLRIVYFDGGRQESYTGPAALTAGTKASQQHNGAQPQV
                     TALPAGVPQKIAQTPELIQIARLGRSGGVAVRGAQEQQRLTPQAQAEVRQARQTYEQM
                     RKSAAANDIVPEQYLYAVLQDHLLYDEMKGVVAEMQKRQPSNPDVAVMAEYVKVKTEP
                     K"
     gene            complement(1762..2340)
                     /locus_tag="VGO02_05300"
     CDS             complement(1762..2340)
                     /locus_tag="VGO02_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477140.1"
                     /translation="MRYWLFACLLLVQPARAQEDVGLVNQMAGEVTFQGGKAKAFMKV
                     REGDRFELPAGAQLRLVYFAGARQESWQGPASLRAGKRESAPLAGKPAAVTILPASVP
                     QRLARIPQLAQGNSVFGGVAVRGTKPAVAQVESEESLREARATYARLRRELPADDLTP
                     ELFLYAALVGGPDPDSEEVRSLAARLRERQER"
     gene            complement(2337..4208)
                     /locus_tag="VGO02_05305"
     CDS             complement(2337..4208)
                     /locus_tag="VGO02_05305"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV7477141.1"
                     /translation="MKGFHKQILRWLGVVAVSTALAMGIARTDGLRAIENIYSDYWHV
                     ITGVRYEPHHTAFVTMDDETLVALKDDPLAFWAPHFGQAMNVLTHAGVKAVGLDFIYQ
                     VSAEGWLKKLNLPDSEISRNYDSPLREALASGNKILITHLVDFPDGSSDLLFPPLDQR
                     LLLPGGINDLGIANLHPDDDKHVRRFQPIMDPNPKAAGVGFAMQLALRAAGKDPSQQQ
                     WEIAGETLRREYGWKTIGYTGPPGTIPTVSMNALLKPDAAADPRVQALKGKVVIIAAN
                     NAGTSDRHFTPYSRGLKSEQMAGGEIHANIVETILSGRYLRELSVGQELAYLALALAV
                     AAWLLLRFSVGGGTALMPLLLLLAVPPAYFAFQVDTVLPVAEVQSGLVAAFLFTFVLR
                     LTGEERERTRQRQIFGRYVADDVVKVLLEEDRAIDLAGEMKMVTVLFSDIRGFTTLSE
                     KLTAHEVVEMLNAYFTRVCEPILAQGGTIDKYIGDAVMAVFGSTVSYPDHARRGLKAA
                     LGMQKEAEGFKEWMLKRFPERGLGEFRIGVGLYTGEALVGNIGAPKRLEFATIGDTVN
                     AASRLEGLTKEMKVVIAASETTVNAAGVGVVIGKIETMTVRGRSEPIRVYEVLGMDG"
     gene            complement(4205..>5749)
                     /locus_tag="VGO02_05310"
     CDS             complement(4205..>5749)
                     /locus_tag="VGO02_05310"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEV7477142.1"
                     /translation="QRERRDQLQERVAQLRNARATVPVAVTNAAERVAEFDAQLTQAS
                     IDLIGVEEQMWRDYPRFMELANPRPVTVEDLQQKLLNPGETLLSYVLLPNEAVIFVVT
                     PVRFVMRVVPVKRAAIAERVHSIRRTIEKVAIGESVLFLREIEPEVLHSLYRDLVAPV
                     GDVLAGREKLLVVADGPLQTIPFELMVATYGAAERSAFERTKAEADGSAQRPYLGEYA
                     ALDYAGRRFRIAYLPSLSALTSQRLYPKRAGGGKLDLVAFADPMFTPDAQHRMPGATL
                     KALAQLNVGGLAKKKGLDGAPLIPRLAETAEEANEIARVLGGKSQIYVGAAAQEKMAK
                     SGELRTARFVLFATHGFLGGEYLGSELPDEENEERRAARVQAQPALALTLVGDLKGED
                     GLLTMKEVIEDLELNAELVALSACNTAGESAQANNGEGFAGLTRAFMYAGAKGLLVSH
                     WSVDSLSTQALMTATFRNIKSGMKALPAVSDAQKELLGGGYSRDGHHFSRAHPFFWAA
                     FVYVGD"
BASE COUNT          856 a         1990 c         1929 g          974 t
ORIGIN      
        1 gttggagaga tagtcgaagc gcatgccgag gatgcggccg ttcgcgtcga gcgccatctc
       61 ggcggtatgc acgatgtcgc gcgcctgctc gtcgccgagg aacacctccg agcgtgtccc
      121 gacccatttc accgggcggc cgagcttctt cgccgcgtaa aggagcgcgc cgtattccgg
      181 gtacgcgttg aagcgcacgc cgaagccgcc gccgacttcc tcggcgacga cgcgcacctt
      241 ttcgggcggc acacccaaaa ccgccgagag ctgccctcgc atcgggcccg tgccctgggt
      301 agtgccgtgc aggaagtaca ggtccctcga ggaatcgtag gagcccaggg cggtgcgcgg
      361 ctccatcgga tttccgatca cgcgcgagtg gtaatcggtg agcttcacga ccttcgccgc
      421 ggctgcgaac gcggcgttgg tcgccgcctc gtcgccgccg gcgaagtcga gcaccagatt
      481 gcccggcgcg cccggatgga gctgcggtgc gcccgcggcc atcgcctgcg cggccgtgac
      541 cgccgcgggc aggtccgcat actcgaccac caccgcctcg gctgcgtcct gcgcctgcgc
      601 cgcggtcccg gctacgatca gggcgatggg ctcgcccacg tgtcgcacca cttcgcaggc
      661 aagcgagggg cgctgcgcca ggcgctgggc ggagccgtcc cgccatttga agggcgccgc
      721 ggccggtatc ggcttgagtc cggcgtccac cagctcctgg ccggtgaata cagcgatcac
      781 gcccgggctt ttcaaagcgt cttctgtttg aatcgaaacg atcttcgcgt gcgcacgatc
      841 cgagcgcagg aaacacccgt acgcctggcc cggcaggttc cagtcggaga cgtagcgccc
      901 ctgaccggtc agcatgcggt ggtcttccag ccggcctttc atggccggat tctagaaaca
      961 aaaaggcccg ccggagcggg cctttcgcaa cgcgaagccg ggattacttc ggctcggtct
     1021 tgaccttgac gtactccgcc atcaccgcca cgtcgggatt ggacggctgg cgcttctgca
     1081 tctcggcgac cacccctttc atctcgtcgt acagcaggtg gtcctgcagc accgcgtaca
     1141 ggtactgctc gggaacgatg tcgttcgcgg ccgcgctctt gcgcatctgt tcgtaggtct
     1201 ggcgcgcctg gcgcacctcg gcctgggcct gcggcgtcag gcgctgctgc tcctgcgcgc
     1261 cgcgcaccgc cacgccgccc gagcggccga gcctggcgat ctggatcagc tccggggtct
     1321 gcgcgatctt ctgcggcacg ccggcgggga gcgccgtcac ctggggctgc gcgccgttgt
     1381 gctgctggct cgccttggtt ccggcggtga gggcggcggg gccggtgtag ctctcctggc
     1441 ggccgccgtc gaagtaaacg atgcgcagct gcgcgccggc ggggatgctg aagcgatcgc
     1501 cttcgcgcat cttcatgaag gccttcgcct tcgcggtatt gctgccgctg gtgtaggaaa
     1561 cgtcaccgct caactgattg accaggccca cgtcctcggc ggcaaatgcg ctcgcccctg
     1621 cgagcaccgc ggcaagtccg agggaccgga tgatcttttt catcgatttt ttctcccaat
     1681 gaggcagcct gaagtctagg gtgggagagt accttcggcg aggacattta tcacgccaaa
     1741 ttcatgagtc ctaacgactt tttacctttc ttgacgttct cgcaggcgcg ctgcgagaga
     1801 tctgacctcc tcgctgtccg ggtcgggccc gccgacgagc gcggcgtaca ggaacagctc
     1861 gggggtcaag tcgtcggccg gcagctcgcg gcgcagcctg gcgtaggtgg cgcgcgcctc
     1921 gcgcaggctc tcctcgcttt cgacctgggc cacagcgggc ttcgtgccgc gcaccgcgac
     1981 gccgccgaac acggagttgc cctgcgccag ctgcggaatg cgcgcgagcc gctgcggcac
     2041 gctggccggg aggatcgtca ctgcggcggg cttgccggcg agcggcgcgc tctcgcgctt
     2101 gccggcacgc aggctcgccg gtccttgcca gctctcctgg cgcgcgccgg cgaagtaaac
     2161 cagccgcagc tgcgctcccg caggcagctc gaagcggtcg ccttcgcgca ctttcatgaa
     2221 ggccttggcc ttgcctccct ggaaagtgac ctcgcccgcc atctggttga ccagcccgac
     2281 gtcctcctgc gcgcgcgcag gctggacaag aagcaaacac gcgaagagcc aatatctcat
     2341 ccgtccatcc cgaggacctc gtacacccgg atcggctccg agcggcctct caccgtcatc
     2401 gtctcgatct tgccgatgac cacgcctacg cccgccgcat tgacggtcgt ctcggacgcg
     2461 gcgatcacga ctttcatctc cttggtgagg ccttccaggc gggaggcggc gttgacagtg
     2521 tcgccgatgg tagcgaactc gaggcgcttg ggcgcgccga tgttgccgac cagcgcttcg
     2581 cctgtgtaca gcccgacacc gatcctgaac tcgcccagcc cgcgctccgg gaagcgcttg
     2641 agcatccact ccttgaagcc ttcggcttcc ttctgcatgc cgagcgccgc cttcagcccg
     2701 cggcgcgcgt ggtcggggta ggacaccgtc gagccgaaca cggccatcac cgcgtcgccg
     2761 atgtacttgt cgatcgtacc gccctgggcg aggatcggct cgcacacgcg ggtgaaatag
     2821 gcgttgagca tctcgaccac ttcgtgggcg gtcagcttct cggacagcgt ggtaaagccg
     2881 cggatgtccg agaacagcac cgtcaccatc ttcatctcgc cggccaggtc gattgcgcgg
     2941 tcctcttcga ggagcacttt caccacgtcg tcagcgacgt agcggccgaa gatctgccgt
     3001 tggcgcgtgc gctcgcgctc ctcgccggtg aggcgcagca cgaaggtgaa caggaaggcc
     3061 gccacgaggc cgctctgcac ttccgctacc ggcagcacgg tgtcgacctg gaacgcgaag
     3121 tacgccggcg gcacggccag cagtagcagt agcggcatga gcgcagtgcc gccgccgacg
     3181 ctgaagcgca gcagcagcca cgccgcgacg gcgagcgcca gcgcgaggta agcgagctct
     3241 tgccctacgc tcagctcgcg cagatagcga cccgagagga tcgtctcgac gatgttggcg
     3301 tggatctcgc cgcccgccat ctgctcggac ttcaggccgc gcgagtacgg cgtgaagtgg
     3361 cggtccgaag taccggcgtt gttcgccgcg atgatcacca ccttgccctt cagggcctgg
     3421 acgcgcggat cggcggccgc gtccggcttg agcagcgcgt tcatcgagac cgtcgggatg
     3481 gtgcccggcg ggccggtgta gccgatggtc ttccagccat actcgcgtct cagggtctca
     3541 ccggcgatct cccactgctg ctgcgacggg tccttgcccg cggcgcgcag cgccagctgc
     3601 atggcgaagc cgacgccggc cgccttcggg ttgggatcca tgatcggctg gaagcggcgc
     3661 acgtgcttgt cgtcgtcggg atgcaggttg gcgataccaa ggtcgttgat gccgccgggc
     3721 agcagcaggc gctggtcgag cggcgggaac agcagatcgg acgagccgtc ggggaagtcg
     3781 acaaggtgcg tgatcaggat cttgttgccc gaggcgagcg cctcgcgcag cggcgagtcg
     3841 tagttgcgcg agatctcgct gtcgggcagg ttgagctttt tcagccagcc ctcggcgctg
     3901 acctggtaga tgaagtcgag ccccaccgcc ttgacgccgg cgtgcgtcag gacgttcatc
     3961 gcctggccga agtgcggcgc ccagaacgcc agcggatcgt ccttgagcgc gaccagggtc
     4021 tcgtcgtcca tggtgacgaa cgcggtgtga tgcggctcgt agcgcacgcc ggtgatcacg
     4081 tgccagtagt cggagtagat gttctcgatc gcgcgcagcc cgtcggttcg cgcgatgccc
     4141 atggcgagcg cggtcgagac ggcgaccacg ccgagccagc gcaggatctg tttatggaac
     4201 cctttcaatc gccgacgtag acgaacgcgg cccagaagaa cggatgcgcg cgcgagaagt
     4261 gatggccgtc gcggctgtag cccccgccga gcagttcctt ctgcgcatcg ctcaccgccg
     4321 gcagcgcctt catcccggac ttgatgttgc ggaaggtcgc agtcatcagc gcctgcgtcg
     4381 acaggctgtc gacgctccag tgggaaacca gcagtccttt cgcccccgcg tacatgaacg
     4441 cgcgcgtcag gccggcgaag ccctcgccgt tgttcgcctg cgcggactcg ccggccgtgt
     4501 tgcacgccga gagcgccacc agctcggcgt tgagctcgag gtcctcgatc acctccttca
     4561 tggtgagcag gccgtcttcg cccttgaggt cgccgaccag cgtgagcgcg agcgccggct
     4621 gcgcctggac gcgcgccgcg cgccgctcct cgttctcctc gtcgggcagc tcgctgccga
     4681 ggtactcccc gccgaggaag ccgtgcgtcg cgaacagcac gaagcgcgcc gtgcgcagct
     4741 ccccggactt cgccatcttc tcctgcgccg cggcgccgac gtagatctgc gacttgccgc
     4801 ccagcacgcg cgcgatctcg ttcgcctcct cggcggtctc ggccaggcgc gggatgagcg
     4861 gcgcgccgtc cagtcccttc ttcttcgcca ggccgccgac gttcagctgc gcaagcgcct
     4921 tgagcgtcgc cccgggcatg cggtgctgcg cgtcgggcgt gaacatcggg tcggcgaacg
     4981 ccaccaggtc gagcttgccg ccgccggcgc gcttcggata cagccgctgc gaggtgagcg
     5041 ccgagaggct tggcaggtag gcgatgcgaa agcgccggcc ggcgtagtcg agcgcggcgt
     5101 actcgccgag gtacgggcgc tgcgcgctgc cgtcggcttc ggccttggtg cgctcgaacg
     5161 ccgagcgctc agccgcgccg taggtggcga ccatcagctc gaacgggatg gtctgcagcg
     5221 ggccgtcggc gacgaccagc agcttctcgc gcccggcgag gacgtcgccc accggcgcga
     5281 ccagatcgcg gtacagcgag tgcagcacct cgggttctat ctcgcgcagg aacagcaccg
     5341 actcgccgat cgccaccttc tcgatcgtgc gccggatcga atgcacccgc tcggcgatcg
     5401 ccgcgcgctt gaccggcacc acccgcatca cgaaacgcac cggcgtcacc acgaagatca
     5461 ccgcttcgtt cggcagcagg acgtaggaga gcagcgtctc gcccgggttg agcagcttct
     5521 gctgcaggtc ctcgaccgtg actggacgcg gattggcgag ctccatgaag cgcggatagt
     5581 cgcgccacat ctgctcctcg acgccgatga ggtcgatcga cgcctgcgtg agctgcgcgt
     5641 cgaattccgc cacgcgctcc gcggcgttgg tgacggcgac cggcacggtg gcgcgggcgt
     5701 tgcgcagctg cgcgacgcgc tcctgcagct ggtcgcgccg ctcgcgctg
//
LOCUS       DASYJA010000250         1913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2125416, whole genome shotgun sequence.
ACCESSION   DASYJA010000250 DASYJA010000000
VERSION     DASYJA010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2125416"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VGO02_05315"
     CDS             <1..207
                     /locus_tag="VGO02_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEV7477143.1"
                     /translation="IFTGQLEMENGYIVTRGGREGFATATSIPGVFAAGDVQDHVYRQ
                     AVTSAATGCMAALDAEKYLDAHAA"
     gene            191..718
                     /locus_tag="VGO02_05320"
     CDS             191..718
                     /locus_tag="VGO02_05320"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEV7477144.1"
                     /translation="MRTPPDDEEFRKALAGVEPLAEPRRKPLARPRPAPLPRQTRRDE
                     RAALAESLAGPLSIDDALDSGEELVYARAGLQRDVLRKLRRGHWVIEDALDLHGMNRL
                     QAAVAVAEFLRRCLQRRVRCVRIVHGKGLGSLNREPVLKAKLRKWLLREEVLAYCQAP
                     AAEGGSGAVLVLLKG"
     gene            complement(732..1070)
                     /locus_tag="VGO02_05325"
     CDS             complement(732..1070)
                     /locus_tag="VGO02_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEV7477145.1"
                     /translation="MKKIEAIVKPFKLDEVREALSEVGVTGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEVVVPNTQVEGAIDAIVKSARTGKIGDGKIFVTSVEHVVRI
                     RTGETNEAAV"
     gene            1151..>1913
                     /locus_tag="VGO02_05330"
     CDS             1151..>1913
                     /locus_tag="VGO02_05330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006759473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7477146.1"
                     /translation="MDPQEWNALAGDQPFVRHEFLCALLETGCAAARSGWLPQFVLLR
                     RAGALAGAMPLFFKSHSYGEYVFDWAWADAHERHGVTYYPKLLCAIPFTPVRGRRLLA
                     AGARERRALAGAALELARRTSSLHGLFVHEDEAELLRAEGMLLRRTVQFHWKNEGYAD
                     FEAFLARLSHARRKNIRQERRRVREAGVSLRWLEGEAIGRAQWEFFHRCYRHTYATHG
                     SSPYLNLQFFLRLGSALQRNVAMVMAERDGRPIAAA"
BASE COUNT          272 a          670 c          654 g          317 t
ORIGIN      
        1 atcttcaccg gccagctgga aatggagaac ggctacatcg tgacgcgcgg cggccgggag
       61 ggcttcgcca ctgcgaccag catcccgggg gtcttcgccg cgggcgacgt gcaggaccac
      121 gtctaccgcc aggcggtgac cagcgccgcg accgggtgca tggcggcgct cgacgccgag
      181 aaatacctcg atgcgcacgc cgcctgacga cgaggagttc cgcaaagccc tcgccggagt
      241 cgaaccgctc gccgagcccc gccgcaagcc gctcgcgcgc ccgcgaccgg ctcccctgcc
      301 gcgccagacg cggcgcgacg agcgcgccgc cctcgccgaa tcgctcgccg gcccgctttc
      361 gatcgacgat gctctcgatt cgggcgagga gctggtctac gcgcgcgcag gcctgcagcg
      421 cgacgtgctg cgcaagctgc ggcgcgggca ttgggtaatc gaggacgcgc tcgacctgca
      481 cggcatgaat cgcctgcagg cggcagtggc cgtcgccgag ttcctgcgcc gctgcttgca
      541 acggcgagtg cgctgcgtgc gcatcgtcca cggcaagggg ctcggctcgc tcaatcgcga
      601 gccggtgctg aaggccaagc tgcggaaatg gctgctgcgg gaggaagtgc tggcctactg
      661 ccaggcgcct gccgccgaag gcggcagcgg cgccgtgctg gtgctgctga aaggctaggt
      721 agcggcttcg gtcagacggc cgcttcgttc gtttcgccgg tgcggatgcg caccacgtgc
      781 tcgaccgagg tgacgaagat cttgccgtcg ccgatcttgc cggtgcgcgc cgacttgacg
      841 atggcgtcga tggcgccttc gacctgcgtg ttggggacca ccacctcgat tttcaccttg
      901 ggcaggaagt cgaccacgta ctcggcgccg cggtacagct cggtatggcc cttctgccgg
      961 ccgaaaccct tcacctcggt gacggtcagg cccgtgacgc cgacttccga aagcgcctcg
     1021 cgcacttcgt cgagcttgaa cggcttcacg atggcttcga tcttcttcat ttagtgtcct
     1081 ccgcgggtcg tggcgtgcat tgtactctag gggcttggag agcatcgagg tcgtagattc
     1141 ccttgcaggc gtggatccgc aggaatggaa cgcgctcgcc ggcgaccagc ccttcgtccg
     1201 ccacgagttc ctctgcgcgc tccttgaaac cggctgcgcc gcggcgcgca gcggctggct
     1261 gccgcagttc gtcctgctgc gacgcgccgg cgccctcgcc ggcgccatgc cgctgttctt
     1321 caagtcgcat tcttacggcg agtacgtctt cgactgggcc tgggccgatg cccacgaacg
     1381 ccacggcgtg acctactacc ccaagctgct gtgcgccatc ccctttacgc cggtgcgcgg
     1441 ccgccgtctg ctcgccgcgg gcgcgcggga gcgccgcgcc ctcgcgggcg ccgcgctcga
     1501 gctggctcga cgcacctcgt cgctacatgg tctcttcgtc cacgaggacg aggccgagct
     1561 gctgcgggcc gagggcatgc tgctgcgccg cacggtgcag ttccactgga aaaacgaggg
     1621 ctacgccgat ttcgaggcgt tcctcgcgcg gctgtcgcac gcgcgacgca agaacatccg
     1681 ccaggagcgc aggcgggtgc gcgaggccgg ggtaagcctg cgctggctgg agggcgaagc
     1741 catcgggcgc gcgcagtggg agttcttcca ccgctgctac cggcatacct atgccacgca
     1801 cggctcgagc ccctacctca acctgcagtt cttcctgcgc ctgggctcgg ctctgcagcg
     1861 caatgtggcg atggtgatgg ccgagcgcga cggccgcccg atcgcggcag cgc
//
LOCUS       DASYJA010000251         2326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2761784, whole genome shotgun sequence.
ACCESSION   DASYJA010000251 DASYJA010000000
VERSION     DASYJA010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2761784"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..798
                     /locus_tag="VGO02_05335"
     CDS             79..798
                     /locus_tag="VGO02_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV7477147.1"
                     /translation="MNQRPEFTRIRAQLGKSLHFRDLRPTDLDALAELGRIRRLNDNE
                     LASAAGRPQEELWVVLAGGLRLSTVTESGDEFVYAILGQGSFYGLGHVIGTRTTNADA
                     RAFGPTELAVMEGEPFLALLDRHPRLWRHAAGLLVRRLSLAMSVVRDVSVAPLDQRIA
                     RRLLGQAMGGGADINGSAPLELRVNQSDLGRMLGASRSRVNAELRRMAGEGLLKIGYR
                     TLTLVDLARLHEIAGPEVFAF"
     gene            complement(795..1532)
                     /locus_tag="VGO02_05340"
     CDS             complement(795..1532)
                     /locus_tag="VGO02_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477148.1"
                     /translation="MYKSCPKCKAESGGADVCPACGLIFAKYLKARVAVPPPEEETKK
                     EPGEDSFVARAKALAFYVPEEVNAWTVYARALLLVAAAVYGGHLALMDVPAWEMSQSF
                     MHLVLLPFHEFGHIFFRPFGEFMTLAGGSLFQVMMPLGLGAYFIVKNRDPFAGSLMLW
                     WAAAGVMDTAPYIYDAWKPQHVLLTGRTGDTGAHDFIDVLGDLGLLRRAQPIGRAVHA
                     FSIAMMLVALAWSAYLLVQQYRRRRAD"
     gene            complement(1544..>2326)
                     /locus_tag="VGO02_05345"
     CDS             complement(1544..>2326)
                     /locus_tag="VGO02_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEV7477149.1"
                     /translation="EMTERVAENLSLENKLRQAIEKEEFVLHYQPKVSLNDRRIVGVE
                     ALIRWQSPELGLVPPGNFIPLLEETGLILQVGSWALRRAALDHRGWVEQGLKAPRVAV
                     NVSQIQLRQRDFVSVVEAAVLDGIAPTGIDLEITESLVMQDVQGNIEKLKAVRGLGIK
                     IAIDDFGTGYSSLAYLAKLPVETLKIDRAFITSMLDDPNTTTLVQTIISLAHSLQLKV
                     IAEGVEREQQAKVLHLLGCDQMQGFLFSKPLPLPELIVLLKA"
BASE COUNT          396 a          801 c          760 g          369 t
ORIGIN      
        1 ggcgcgcggc ggcaggcgct gggacacctc cagcctgatg cacctcctga tgaaggattg
       61 agacgcccgg ggaggggcat gaaccagcga ccggaattca cccgcatccg cgcccagctc
      121 gggaaaagcc tgcatttccg cgacctgcgg ccgaccgacc ttgacgcgct cgccgagctc
      181 ggccggatcc gccggctcaa cgacaacgag ctcgccagcg cggccggccg cccgcaggag
      241 gagctgtggg tggtgctggc gggcgggctg cgcctcagca cggtcaccga gtccggcgac
      301 gaattcgtct acgccatcct cggccaggga agcttctacg gcctgggcca tgtgatcggc
      361 acgcgcacca cgaatgccga tgcgcgcgcc ttcggtccca ccgagctggc ggtcatggag
      421 ggcgagccgt ttctcgcgct gctcgaccgc catccgcgcc tgtggcggca cgccgccggc
      481 ctcctggtgc gccggctctc cctcgccatg tcggtggtcc gcgacgtcag cgtcgcgccg
      541 ctcgaccagc gcatcgcgcg gcgcctgctc ggccaggcga tgggcggcgg cgccgacatc
      601 aacggcagcg cgcccctcga gctgcgcgtg aaccagtccg acctcgggcg catgctcggc
      661 gcgagccgct cgcgggtcaa cgccgagctg aggcgcatgg caggcgaagg cctgctcaag
      721 atcggctacc gcacgctgac cctggtcgac ctggcgcgcc tgcacgagat cgccggcccc
      781 gaggtgttcg ccttctagtc ggcacgcctg cgccggtact gctgcacgag caggtacgcg
      841 gaccaggcca gcgcgaccag catcatcgcg atgctgaatg catggaccgc gcgcccgatc
      901 ggctgtgcgc gacgcaacag gccgaggtcg ccgagcacgt cgatgaagtc atgcgccccg
      961 gtatcgccgg tgcggccggt gagcagcacg tgctgcggct tccatgcgtc gtagatgtag
     1021 ggcgccgtgt ccatcacccc cgccgccgcc caccacagca tcagcgagcc ggcgaagggg
     1081 tcgcggttct tgacgatgaa ataggcgccg agcccgagcg gcatcatcac ctggaacagc
     1141 gaaccgccgg cgagcgtcat gaattcgccg aacggccgga aaaagatgtg gccgaactcg
     1201 tggaacggca gcagcacgag gtgcatgaac gactgcgaca tctcccaggc cgggacgtcc
     1261 atcagcgcga ggtggccgcc gtacactgct gccgcaacga ggagcagcgc ccgcgcgtac
     1321 acggtccagg cattgacttc ctcgggaacg tagaaggcga gcgccttggc tcgcgccacg
     1381 aaggaatcct cgcccggctc ttttttcgtt tcttcttctg gaggcggaac cgcgacccgc
     1441 gccttgagat atttcgcgaa gatcaagccg caggcggggc agacgtccgc gccgcccgac
     1501 tccgctttgc acttcgggca gctcttatac atcactaagc ctatcaggcc ttgagcagaa
     1561 cgatcagctc cggcaggggc agcggcttgc tgaacaggaa tccctgcatc tggtcgcagc
     1621 ccaggagatg cagcaccttc gcctgctgtt cccgctcgac gccttcagcg atcaccttga
     1681 gctgcagcga gtgggcgagc gagatgatgg tctgcaccaa ggtggtggta ttggggtcat
     1741 cgagcatcga ggtgatgaag gcgcggtcga tcttcagcgt ttccaccggc agcttggcga
     1801 ggtaggcgag cgaggaatag cccgtgccga agtcgtcgat ggcgatcttg atgcccaaac
     1861 cgcgcaccgc cttcagcttc tcgatattgc cctgaacgtc ctgcatcacc aggctttccg
     1921 tgatctcaag atcgatcccg gtcggcgcga taccgtcgag caccgcggcc tccaccacgg
     1981 acacgaagtc tcgctgccga agctggatct gcgagacgtt gacggccacc cggggcgcct
     2041 tcagtccctg ctcgacccag ccacggtgat cgagcgccgc ccgccgcagc gcccaggagc
     2101 cgacctgcag gatcaagccg gtttcctcca gcagcggaat gaagttgccg ggcggcacga
     2161 ggccgagctc ggggctctgc cagcggatca gcgcctcgac gccgacgatg cgcctgtcat
     2221 tcagactcac cttaggctgg tagtgcagga cgaactcctc cttctcgatc gcctggcgaa
     2281 gcttgttctc gagcgagagg ttctccgcga cgcgctcggt catctc
//
LOCUS       DASYJA010000252         2188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2854589, whole genome shotgun sequence.
ACCESSION   DASYJA010000252 DASYJA010000000
VERSION     DASYJA010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2854589"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VGO02_05350"
     CDS             <1..433
                     /locus_tag="VGO02_05350"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEV7477150.1"
                     /translation="ARGVHAGASLVVNAGTATTVDMLAGDGSFLGGAILPGVELMRFV
                     LHEHTGRLPIQEGNFRDMPRNTVDAIETGARHAQAGAVERMYRAFRDMDGTPLCIVSG
                     GAGRVLADQLAMPRRYVENLVLEGLVRIALSERAAAGSAAK"
     gene            430..1116
                     /locus_tag="VGO02_05355"
     CDS             430..1116
                     /locus_tag="VGO02_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEV7477151.1"
                     /translation="MRTLFLVLVLANLALFAWWRYLSPADSGLDRLPLARQIEPDKLK
                     IVPPAELTRAPAKKAAPPAAAPASQAATPGAQSCLEWGSFTLADAPRMEKALEPLGLG
                     ERLAQRRTEETAGWWVFIPSQGSRQAALKKVVELKALGVEEFFVLAEDSQFRWALSLG
                     VFRNEDAAQARLAALRNQGVRTARVGPRELVVPKVWLQVKSVDAATEARLKEIAGTID
                     GSELRGCPPG"
     gene            complement(1130..1510)
                     /locus_tag="VGO02_05360"
     CDS             complement(1130..1510)
                     /locus_tag="VGO02_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7477152.1"
                     /translation="MAASILVVDDDESIRDLLRLHLSAAGYEVHVAADAISAGYMVLR
                     SPPDLIITDINMPHMDGFEFIAALKSDKSLPRIPVIFLTTVKDGDLRGKELGAVSYVT
                     KPVRSDRLLSLVAQHVPGGVQPIG"
     gene            complement(1523..1595)
                     /locus_tag="VGO02_05365"
     tRNA            complement(1523..1595)
                     /locus_tag="VGO02_05365"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1560..1562),aa:Thr,seq:cgt)
     gene            1764..>2188
                     /locus_tag="VGO02_05370"
     CDS             1764..>2188
                     /locus_tag="VGO02_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEV7477153.1"
                     /translation="MRLARGFTLIELLIVVAVISILAAIAYPNYTDYVRRGNLQEAFA
                     NLSDYRVRMEQFYQDNRSYANAGNCGAAAIPGGNIKFFSYPCALNTTPLAPAGQSYRM
                     TATGTAGSVSGFVFDINERNVRRTQATPAWGAVPADGNIR"
BASE COUNT          339 a          742 c          778 g          329 t
ORIGIN      
        1 cgcgcgcggc gtgcacgcgg gcgcgagcct ggtggtgaac gcgggcacgg caaccaccgt
       61 ggacatgctg gcgggcgacg gcagcttcct cggcggcgcc atcctgcccg gcgtcgagct
      121 gatgcgcttc gtgctgcacg agcacaccgg ccggctgccg atccaggagg gcaacttccg
      181 cgacatgccg cgcaacaccg tcgacgccat cgagaccggc gcgcgtcacg cccaggcggg
      241 cgcggtcgag cgcatgtacc gcgccttccg cgacatggac ggcacgccgc tttgcatcgt
      301 ctcgggcggc gccgggcggg tgctcgccga ccagctcgcc atgccgcggc gctacgtcga
      361 gaacctggtg ctcgagggcc tggtgcgcat cgcgctgagc gagcgcgcgg cggccggcag
      421 cgcggcgaaa tgaggacgct cttcctggtc ctggtgctcg ccaacctggc gctgttcgcc
      481 tggtggcgct acctgtcgcc ggcggattcc ggactcgacc ggctgccgct cgcgcgccag
      541 atcgagcccg acaagctgaa gatcgtgccg ccggccgagc tcaccagggc gccggcgaag
      601 aaggcggcgc cgcctgccgc ggcgccggcg tcccaggcgg ctacgccggg cgcgcagtcc
      661 tgcctcgagt ggggcagctt taccctcgcc gacgcgccgc gcatggaaaa ggcgctcgag
      721 ccgctcggcc tgggcgagcg cctggcgcag cggcgcaccg aggagacggc aggctggtgg
      781 gtattcatcc cgtcacaagg cagccggcag gccgcgctga agaaggtcgt cgagctgaag
      841 gcgttgggcg tcgaggagtt cttcgtgctg gccgaggaca gccagttccg ctgggcgttg
      901 tcgctgggcg tgttccgcaa cgaggacgcg gcgcaggcca gactcgcggc gctgcgcaac
      961 cagggcgtgc gcactgcgcg cgtcgggccg cgcgagctgg tggtgccgaa ggtgtggctg
     1021 caggtgaaga gcgtcgacgc ggcgaccgag gcgcggctga aggagatcgc ggggacgatc
     1081 gacggcagcg agctgcgcgg ctgcccgccg ggctagtcag cccgcagttt caccctatgg
     1141 gctgcacgcc cccgggcacg tgctgcgcga ccagcgagag cagccggtcc gagcgcaccg
     1201 gcttggtgac gtagctgacc gcgcctagct ccttgccgcg caggtcgccg tccttgaccg
     1261 tggtgaggaa gatcaccggg atgcgcggca gcgacttgtc cgacttgagc gccgcgatga
     1321 actcgaagcc gtccatgtgc ggcatgttga tgtcggtgat gatcaggtcg ggcgggctgc
     1381 gcagcaccat gtagcccgcc gagatggcgt ccgccgcgac gtgcacctcg tagccggcgg
     1441 cgctcaggtg caggcgcagc aggtcccgga tgctttcgtc gtcgtccact acgagaatgc
     1501 tggcggccat ggattgactc ggtgctggcg gcgtgactcg aacacgcgac ctgacgctta
     1561 cgaagcgact gctctaccaa ctgagctacg ccagcagagc gactgattat aaccaccccg
     1621 ggaggcaccc cccgggaacg tgaactagta ccgatatact gcgccgcatc gcgccgttcg
     1681 gtgcgcgatt ccagccgccg gtcgcctgtt tttgcaggtc cgcagccggc tacctagcat
     1741 tccatctcaa caagggaggc agtatgcgtc tagcaagggg cttcacgctt atcgagctgt
     1801 tgatcgtggt cgccgtgatc tcgatcctgg cggctatcgc ttacccgaac tacaccgact
     1861 acgtgcggcg gggcaacctg caggaggcgt tcgccaacct ctcggactac cgcgtgcgca
     1921 tggagcagtt ctaccaggac aaccggagct acgccaacgc cggcaactgc ggcgcggcgg
     1981 cgatccctgg cggcaacatc aagttcttct cgtacccgtg cgcgctcaac acgacgcccc
     2041 tcgcgccggc cggccagtcc tacaggatga cggcgaccgg gacggcgggc agcgtgtcgg
     2101 gcttcgtttt cgacatcaac gagcgcaatg tgcgccgcac ccaagcgacg cctgcctggg
     2161 gcgcggtgcc ggccgatggc aacatccg
//
LOCUS       DASYJA010000253         3712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_310351, whole genome shotgun sequence.
ACCESSION   DASYJA010000253 DASYJA010000000
VERSION     DASYJA010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310351"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VGO02_05375"
     CDS             complement(<1..778)
                     /locus_tag="VGO02_05375"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7477154.1"
                     /translation="MKWLALVLALVSTAAGAQTYPSKPIRWIVGFPPGGGTDIISRLL
                     ALKLGETLSQQVVVENRPGATGMIGANAVAKSAPDGYTLLMGHVNSNAIAPNLVEKPL
                     YDPLKDFAPIAYVGYVPNVLTINPAVTPVKTIQELIALAKAQPGKLTFASPGVGSTNH
                     LAGEMFKLGAGIDIVHVPYKGSGPAIIDLLGGQITMNFDAMSSVVQYLKDGRMRALAV
                     TTAEPDPQYPTVPTVRQAGFKDFEVTNWYGVMAPAGTPREV"
     gene            complement(786..1652)
                     /locus_tag="VGO02_05380"
     CDS             complement(786..1652)
                     /locus_tag="VGO02_05380"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7477155.1"
                     /translation="MERKPDIPPPVADTHAPREKFPPKATDCHAHVFGPETPLLPKTH
                     FVPHPCPLADYVRMLRKLGCERAVLVQPSVYGTDNGMIAAALRSKVFPLRAVAVVAHD
                     VSDRELEDMHAEGFRGVRINTASATKGLKLEHAARLAERLRPMGWHLQFFVNLRQTPQ
                     IEEQLAKLKITIVIDHFARIGAADGLDAPPFQALLRLLRRDNCWAKVMGPYFASEQFP
                     RYPDLAPFARAMVAAAPDRIVWGTDWPHASAREKMQNDGDYADMLIDWVPDAAQRRAI
                     LVTNPERLYSFQ"
     gene            complement(1654..2550)
                     /locus_tag="VGO02_05385"
     CDS             complement(1654..2550)
                     /locus_tag="VGO02_05385"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEV7477156.1"
                     /translation="MERQTTRLRKLLRDKTFLHMPSVYDAIGGRLVQSLGFEAAYVGG
                     YVTGGSKAVTEPLLTMTEQVDLAGEVAAATTFPVLADAGAGFGDALHTMRTVREFIHA
                     GVAGVHIEDQLFPKRAHYHKYVVHGVPVEEFVEKIRYSIKQRDESDKDFVVIARTDTC
                     RALGLEEASMRLNRAADAGADMGLLFPRNLDDAEKAPKACRLPLVYVQSRGNRDGRPI
                     ISRKDLQQMGYVACIEAQVVLCTAFHFLKKALAELRQTGEYKGMSQAEYVASRQQVED
                     LIDLSKYYEIESQTVEARQQQK"
     gene            complement(2614..2850)
                     /locus_tag="VGO02_05390"
     CDS             complement(2614..2850)
                     /locus_tag="VGO02_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477157.1"
                     /translation="MAANMLGGPRKLRHFLKASSADVANWLSGVKEPPTQVFLAALEL
                     ILDDLDSGGHQLRKVNAPRRKSAIILKARKPTLK"
     gene            3048..3326
                     /locus_tag="VGO02_05395"
     CDS             3048..3326
                     /locus_tag="VGO02_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477158.1"
                     /translation="MSRENRTIPLVRELSAAARDCPLAPPETASVHSRTLHRACVILG
                     GIAQLAKHLEVAEADLTGWMRGGVQVPEPVFLAAVEVLLLYASKGQSN"
BASE COUNT          575 a         1186 c         1246 g          705 t
ORIGIN      
        1 tgacttcgcg cggcgtgccg gcgggcgcca tcacgccgta ccagttggtc acctcgaagt
       61 ccttgaagcc cgcctgacgc acggtcggca cggtggggta ctgcgggtcg ggctcggcag
      121 tggtgacggc cagtgcgcgc atgcggccgt ccttgaggta ctgcaccacc gaggacatgg
      181 cgtcgaagtt catcgtgatc tggccgccca gcaggtcgat gatcgccggg ccgctgccct
      241 tgtacggcac gtgcacgatg tcgattcccg cgccgagctt gaacatctcg ccggcgaggt
      301 ggttggtgct gccgacccct ggcgaggcga aggtgagctt gccgggctgc gctttggcga
      361 gcgcgatcag ctcctggatg gtcttcaccg gcgtgaccgc ggggttgatc gtcagcacgt
      421 tgggcacgta gccgacgtag gcaatcggcg cgaagtcctt cagcgggtcg tacagcggct
      481 tttccacgag gttgggcgcg atcgcgttgg agttcacgtg ccccatcagc agcgtgtagc
      541 cgtcgggcgc gctcttcgcg accgcgttgg cgccgatcat gccggtcgca ccggggcggt
      601 tctccaccac cacctgctgc gacagcgtct cgcccagctt cagcgccagc aggcgcgaaa
      661 tgatgtcggt gccgccgccg ggcggaaagc cgacgatcca gcggatcggc ttggacgggt
      721 aggtctgcgc gcctgccgcg gtggagacga gggcaaggac cagtgcgagc catttcatgg
      781 gagcctcatt ggaaggagta cagccgctcg gggttggtca ccagaatcgc cctgcgctgg
      841 gccgcatcgg gtacccagtc gatcagcatg tcggcgtagt cgccgtcgtt ctgcatcttc
      901 tcgcgcgcgc tggcgtgggg ccagtctgtg ccccacacga tccggtccgg cgccgcggct
      961 accatggcgc gcgcgaaggg cgcgaggtcc ggatacctgg ggaactgctc cgacgcgaag
     1021 tacggtccca tcaccttggc ccagcagttg tcacgccgca gcagcctgag gagagcctgg
     1081 aagggcgggg cgtcgagccc gtcggcggcg ccgatgcggg cgaagtggtc gatgacgatg
     1141 gtgattttca acttcgcgag ctgttcctcg atctgcggcg tctgccgcag gttgacgaag
     1201 aactgcagat gccagcccat cgggcgcagg cgctctgcga gccgcgcagc gtgctcgagc
     1261 ttgagcccct tcgtggccga ggcggtgttg atccgaacgc cgcggaagcc ttctgcatgc
     1321 atgtcctcga gctcgcggtc gctcacgtcg tgcgccacca cggccaccgc gcgcagcgga
     1381 aagaccttcg agcgcagcgc cgcggcgatc atgccgttgt ccgtgccgta gacgctcggc
     1441 tgcaccagca ccgcgcgctc gcagcccagc ttgcgcagca tccgcacgta gtccgcgagc
     1501 gggcaggggt gaggcacgaa atgcgtcttc ggcagcagcg gcgtctccgg cccgaagacg
     1561 tgcgcgtggc agtcggtggc cttgggcggg aatttctccc gtggcgcatg tgtgtccgcc
     1621 accggcggcg ggatgtcggg cttgcgctcc atgctacttc tgctgctggc gcgcctcgac
     1681 ggtctgcgac tcgatctcgt agtacttgct gaggtcgatc aggtcctcca cctgctggcg
     1741 cgaggccacg tattcggctt gcgacatacc cttgtactcg cccgtctggc gcagctccgc
     1801 gagtgccttc ttcaggaagt ggaacgccgt gcacagcacg acctgcgctt cgatgcaggc
     1861 gacgtagccc atctgctgca ggtccttcct ggagatgatc ggccggccgt cgcggttgcc
     1921 gcggctttgc acgtagacga gcggcaggcg gcacgccttc ggcgccttct ccgcgtcgtc
     1981 gaggttgcgc gggaacagca gccccatgtc ggcgccggcg tcggcggcac ggttgagccg
     2041 catgctcgcc tcttccaggc cgagcgcgcg gcaggtgtcg gtgcgggcga tgaccacgaa
     2101 atccttgtcg ctctcgtcgc gctgcttgat cgaataacgg atcttctcga cgaactcctc
     2161 gaccgggacg ccgtgcacaa cgtacttgtg atagtgcgcg cgcttcggga acagctggtc
     2221 ctcgatgtgc accccggcca cgccggcgtg gatgaactcg cgcaccgtgc gcatcgtgtg
     2281 cagcgcgtcg ccgaaacccg cgccggcgtc ggcgagaacc gggaaggtcg tggccgccgc
     2341 cacctcgccg gcgaggtcca cctgctcggt catggtgagg agcggctcgg tgacggcctt
     2401 cgagccgccg gtcacgtagc cgccgacata cgcggcctcg aagcccaggg actgcacgag
     2461 gcggccgccg attgcgtcgt ataccgatgg catatgcagg aaagtcttgt cgcggaggag
     2521 cttgcgcagc cgcgtggtct gtcgttccat gggattcctc gaggtggttt cgctcagtca
     2581 ttaggccaga gttgcgccgc gtcaaccggc ttttcacttc aacgtcggtt tgcgcgcctt
     2641 caggatgatt gcggattttc ggcgcggcgc attgacctta cgaagttggt gtccaccact
     2701 gtcgagatcg tccaggatca gttctagtgc agccaagaaa acttgagtcg gcggttcctt
     2761 cacgcccgac aaccagttcg ctacgtcggc cgacgaagcc ttaaggaagt ggcgcagctt
     2821 gcgcgggccc ccgagcatat tcgccgccat ccgtaacgcc atcgttttta ggcctagggc
     2881 cacaccccta gggttatctt ttcctccggg ctctcacaag tcgccgaccg ttgattttcc
     2941 ttgtatccgg attagcgtcg ggcaaggccc gacggaagtg gaaccgggct tgccaagggt
     3001 cgacccaggg gttatatgtg ccatgtcttc aggatggcca cctgagtatg agccgcgaaa
     3061 accgcacgat tccgctagtg cgagaactgt ccgccgctgc gcgggattgt ccgctggcgc
     3121 cgcccgaaac ggcgagcgtt cattcgcgca ccctacaccg cgcctgcgtg atcttgggcg
     3181 gcatcgccca gctagcgaag caccttgaag tcgcggaagc tgatctgacg ggttggatgc
     3241 gcggtggcgt ccaggttccc gagcccgtgt ttctcgctgc cgttgaggtg cttcttctgt
     3301 acgcctctaa gggtcaatcg aactgacgcc cgcctccggt tggccggcgt tgacttcgga
     3361 ctagtaccgc gtcgctgagg aatcgccaga agaggagcag tcgcggggcg ctgctggatc
     3421 atccgagccc gtgtcgctgg gcggcgcggg cgtttcctgc aggatcaagt cgactgctcg
     3481 aaggaaaatg cccatcgggg ggacgctcct gtcggcaatc cagttttcca gatcggcgac
     3541 cggaacaccc aaatggcgac gcaacttggc gcgcccgcca attaggtccg atgccttctg
     3601 gaaggtcctg ctgtagacac tggcagccac tagcgctctt tcggggcgtt gttatccgcg
     3661 ccgatgatcg ctcaatgtgc gtgaacgcac aatcgtggcg ctagttagcc cg
//
LOCUS       DASYJA010000254         1913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1673152, whole genome shotgun sequence.
ACCESSION   DASYJA010000254 DASYJA010000000
VERSION     DASYJA010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1673152"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..129
                     /locus_tag="VGO02_05400"
     CDS             52..129
                     /locus_tag="VGO02_05400"
                     /inference="COORDINATES: protein motif:HMM:NF014822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477159.1"
                     /translation="MACDGHQHSLTLTLPPLGALFLQPA"
     gene            101..>1913
                     /locus_tag="VGO02_05405"
     CDS             101..>1913
                     /locus_tag="VGO02_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEV7477160.1"
                     /translation="MERSSSNLHSSAAQREALPPRVVIESPRPEVDCGRYPAKRVVGE
                     DVLVEADVFTDGHDVVQCELLFRFQGEKEWQTAPMQFIGNDHWSASFRVEQLGRYEYT
                     VRGWADPLETWKRDLEKRKAAGQDTTLDLQIGAEIEKRTERAVSYYDKVLEVVVDPPR
                     ARFSSWYEMFPRSARGDGTHGTFRDVIARLPYVAELGFDVLYLPPVHPIGTTARKGKN
                     NDVAALPGDVGSPWAIGAKEGGHKAIHPQLGSFADFDLLVKKAKEHDIDIALDIAFQC
                     SPDHPYVKEHPQWFRWRPDGTVQYAENPPKKYQDIYPFEFETAAWPGLYDELKSVFEF
                     WFKHGVKIFRVDNPHTKPFAFWEWCIGELKREHPELIFLSEAFTRPRIMHRLAKLGFT
                     QSYTYFTWRNTKEELVEYFTELSSDPSREYFRPNVWPNTPDILHATLQHGGPPAFASR
                     VILAATLSANYGIYGPAYELLEHQPREPGSEEYLFSEKYEIKRWDLDRSDSLKSLIKK
                     LNEIRKANPALQSDWNLRFHEISNDQLIAYSKQAGENLILVVVNLDTRYVQSGWVEFG
                     FGGEAFEVQDLLGGGRYKWQGEKNYVQLNPQELPAPQV"
BASE COUNT          377 a          599 c          601 g          336 t
ORIGIN      
        1 gagtacggcg gcagcggcag cggcaacttc ggcggcgtcg aggccgaagc ggtggcctgc
       61 gacggccacc aacattccct cactctgacc ctgcctccgc ttggagcgct cttcctccaa
      121 cctgcatagc tcggcggcgc agcgcgaagc gctgccgccg cgcgtagtga tcgagtcgcc
      181 gcgtcccgag gtcgactgcg gccgctatcc ggccaagcgc gtcgtcggcg aggacgtgct
      241 cgtcgaggcg gatgtcttta cggatgggca tgacgttgtt caatgcgaat tgctgtttcg
      301 tttccagggg gaaaaggaat ggcagaccgc ccccatgcaa ttcatcggca acgatcattg
      361 gagcgcctca ttccgcgtcg agcagctggg gcgctacgag tacacggtgc ggggctgggc
      421 cgatccgctc gaaacctgga agcgcgactt ggagaagcgc aaggccgccg gccaggacac
      481 cacgctcgac ctgcagatcg gcgccgagat cgagaagcgc accgagcgcg cggtctcgta
      541 ctacgacaag gtcctggagg tcgtggtcga cccgccgcgc gcgcgcttct cgagttggta
      601 cgagatgttt ccgcgctcgg cgcgcggcga cggcacgcac ggcaccttcc gcgacgtcat
      661 cgcgcgcctg ccgtacgtcg ccgagctcgg cttcgacgtc ctctacctcc cgccggtcca
      721 tcccatcggg acgaccgcgc gcaagggaaa gaacaacgac gtggccgccc tgccgggcga
      781 tgtcggcagt ccctgggcca taggcgcgaa ggagggcggg cacaaggcca tccatcctca
      841 actgggttct ttcgcggatt ttgatttgct cgtaaaaaaa gcgaaagaac acgacatcga
      901 catcgccctg gacatcgcat tccagtgctc gcccgaccat ccgtacgtca aggagcaccc
      961 gcagtggttc cgctggcggc cggacggcac ggtgcagtac gccgagaatc cgccgaagaa
     1021 ataccaggat atctacccgt tcgagttcga gacggccgcg tggcccgggc tctatgacga
     1081 gctgaagagc gtgttcgaat tctggttcaa gcacggcgtg aagatcttcc gagtggacaa
     1141 cccgcacacc aagccgtttg ccttctggga atggtgcatc ggcgaattga agcgggagca
     1201 ccccgagctg atctttctct cggaagcctt cacccggccg cgcatcatgc accggctggc
     1261 gaagctcggc ttcacgcagt cctatacgta cttcacgtgg cgcaatacga aggaagagct
     1321 ggtcgagtat ttcaccgagc tgtcttcgga tccctctagg gagtacttca ggcccaacgt
     1381 ctggccgaac acccccgaca tcctgcacgc cacgctgcag cacggcggtc cgccggcgtt
     1441 cgcgtcgagg gtgatccttg ccgcgaccct ctcggcgaac tacggcatct acggcccggc
     1501 ctacgagctg ctggagcacc agccgcgcga gccgggcagc gaggagtatc tcttctcgga
     1561 gaagtacgag atcaagcgct gggatcttga tcgttccgat tcattgaaat ccctgataaa
     1621 aaaattaaat gaaattcgaa aagcgaaccc ggcgttgcag tcggactgga atctgcggtt
     1681 ccatgagatc agcaatgacc agttgatcgc gtattcgaag caagcaggcg aaaacctgat
     1741 cctcgtcgtc gtcaacctcg atacccgcta cgtgcagtcg ggctgggtcg aattcggctt
     1801 cggcggcgag gcgttcgagg tgcaggacct gctggggggc gggcgctaca agtggcaggg
     1861 ggagaagaac tacgtccaac taaaccctca ggagctgcct gcgccgcagg tgg
//
LOCUS       DASYJA010000255         1570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2581396, whole genome shotgun sequence.
ACCESSION   DASYJA010000255 DASYJA010000000
VERSION     DASYJA010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2581396"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VGO02_05410"
     CDS             complement(<1..396)
                     /locus_tag="VGO02_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HEV7477161.1"
                     /translation="MKKVYPDAASALKGLVRDNITIAAGGFGLCGIPENFIRALVDSG
                     VKGLTIIGNNAGVDDFGMGLLLKSRQVKKVIASYVGENKEFERQVLAGELELQLTPQG
                     TLAEKFRAGGAGIPGFYTRTAFGTKLAEGK"
     gene            complement(408..842)
                     /locus_tag="VGO02_05415"
                     /pseudo
     CDS             complement(408..842)
                     /locus_tag="VGO02_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891596.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
     gene            complement(839..1255)
                     /locus_tag="VGO02_05420"
     CDS             complement(839..1255)
                     /locus_tag="VGO02_05420"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEV7477162.1"
                     /translation="MAQKKLMHVGKLVHVERIAIRWGDMDAMGHVNNTVYFRFMEQAR
                     IGWFDALVPRGEAWRATGIVIANASCNFKRPINYPGTVEVKLLVGAPGSSSVPTYYEL
                     RVDEELYADGEATVVFIDMQKQKPIRIPQNIREALQ"
     gene            1281..>1570
                     /locus_tag="VGO02_05425"
     CDS             1281..>1570
                     /locus_tag="VGO02_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014725605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7477163.1"
                     /translation="MSRESMQYDVVVVGGGPAGLAAAIRVKQRSPDTSVCVLEKGSEI
                     GAHILSGAVMDPRAIAELFPDWKEKGAPLNVPVLEDRFLFLSKEKSFRTPNFL"
BASE COUNT          243 a          517 c          494 g          316 t
ORIGIN      
        1 cttgccttcc gcgagcttcg tgccgaaggc ggtgcgcgtg tagaagcccg ggatgccggc
       61 gccgcccgcg cggaatttct ccgcgagcgt gccctggggc gtgagctgga gctcgagctc
      121 gcccgccagc acctggcgct cgaactcctt gttctcgccg acgtaggagg cgatcacctt
      181 cttcacctgg cgcgatttca gcagcaggcc catgccgaaa tcgtcaaccc cggcgttgtt
      241 gccgatgatc gtcaggccct tcacgccgct gtccaccagc gcgcggatga aattctccgg
      301 gatgccgcac aggccgaagc cgcccgcggc gatggtgatg ttgtcgcgca cgaggccctt
      361 cagggccgac gcggcgtccg ggtacacctt tttcatctga tgccctcttc gagcacgtac
      421 tccttgccgt cgaaggcctt ggtttccttg ccgtcggcga gcttggtgcc cgcggcggtg
      481 cgcgtgtaga agccggggat gccggcgccg cccgcgcgca gcttctcggc gagcgtaccc
      541 tgcggcgtga gctgcagatc gagctcgccg gcgagcacct ggcgctcgaa ctccttgttt
      601 tcgccgacgt aggaggcgat caccttcttc acctggcggg tcttcagcag caggcccatg
      661 ccaaagtcgt cgacgccggc gttgttgccg acgatggtga gccccttcac cgcgctgtcg
      721 cgcagcgcgg cgatgaaatt ctcgggaatg ccgcacaggc cgaagccgcc ggcggcgatg
      781 gtcatgttgt cgcgcagcag ccctttcagg gctgccgcgg cgtcgggata aaccttcttc
      841 attggagtgc ctctcggatg ttctgcggaa tgcggatcgg cttctgcttt tgcatgtcga
      901 tgaatacgac cgtcgcctcg ccgtcggcgt agagctcctc gtccacccgg agctcgtagt
      961 acgtcggcac gctggagctt ccgggcgccc ctaccagcaa tttcacttcc accgtgcccg
     1021 ggtagttgat cggccgcttg aagttgcacg aggcgttggc gatgacgatg ccggtcgccc
     1081 gccacgcctc gccgcgcggc accagcgcgt cgaaccagcc gatgcgcgcc tgctccatga
     1141 agcggaagta gacggtgttg ttgacgtggc ccatcgcgtc catgtcgccc cagcggatcg
     1201 caatgcgctc gacgtgcacc agctttccga catgcatcag cttcttttgg gccatggctg
     1261 actgattata attgcgcgcg atgagccgcg aatcgatgca gtacgacgtg gtcgtcgtcg
     1321 gcggcgggcc ggccggcctc gccgccgcga tccgcgtgaa gcagcgctcg cccgacacct
     1381 cggtgtgcgt gctcgagaaa ggctccgaga tcggcgccca catcctgtcc ggcgcggtga
     1441 tggatccccg cgccatcgcg gaactcttcc cggattggaa agagaaaggc gccccgctga
     1501 atgttcctgt tttggaagat agattcttgt ttttatcaaa agaaaaatct ttcaggacac
     1561 ccaactttct
//
LOCUS       DASYJA010000256         2805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2672321, whole genome shotgun sequence.
ACCESSION   DASYJA010000256 DASYJA010000000
VERSION     DASYJA010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2672321"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /gene="msrA"
                     /locus_tag="VGO02_05430"
     CDS             complement(<1..466)
                     /gene="msrA"
                     /locus_tag="VGO02_05430"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEV7477164.1"
                     /translation="MFAFAKKLKSLLPPQNTIAGRSTPIEVPEKHFVNGHPLKAPFPA
                     GLQLAQFGMGCFWGAERLFWQTPGVYSTAVGYSGGETKNPTYEEVCSGSTNHAETVLV
                     VFDPKKISYEQLLKVFWEGHNPTQGMRQGNDAGTQYRSAIYTYGEMQNAAALA"
     gene            494..1480
                     /locus_tag="VGO02_05435"
     CDS             494..1480
                     /locus_tag="VGO02_05435"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEV7477165.1"
                     /translation="MKICIYGAGAVGGLMAAWLTRSSGNEVSAVARGPQLEALRKDGL
                     RVRDRASGKMGTFSILASDDPAVLGPQDYVIVAVKAQNLTQVAAGIAPLLGPDTSIVT
                     AMNGVPWWFFDCLKYGGGRQRLESLDPDGSLSRAMPTQRIVGCVVHLAASTPEPGLIS
                     HNMGKKLILGQPGGANTTRVKRIANALTIAGFEIAVTGSIEKEFWIKLLGNVSFNPVS
                     ALTQSTADVLIENREVREYMVEIMREVLAIGRAVGVDAAIDPEARIDMARVLGPFKTS
                     MLQDLEAGKRLEIDGLLAGTLEIARKAGVKAPYTESLFGLIRARADATKQYR"
     gene            1477..2094
                     /locus_tag="VGO02_05440"
     CDS             1477..2094
                     /locus_tag="VGO02_05440"
                     /inference="COORDINATES: protein motif:HMM:NF018684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HEV7477166.1"
                     /translation="MKYGALILFIFCSGAFAQYSGPAVQACLDYAKREQKQNIVLERD
                     QNLLIERYTRKLGSQFVSSILSGNGAVVYDGAPSSELSFVCLLADDKRAVFFQWVARP
                     NAPATMQCLRNPDLQKSPRACFDLMLNVAEQDLTLVYAQRFQEANERGEAPLAAFRKS
                     NDEWRQYRDAECARRRDFAPTGVSPDDYAIACTVELTRRRALDMR"
     gene            2097..2717
                     /locus_tag="VGO02_05445"
     CDS             2097..2717
                     /locus_tag="VGO02_05445"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEV7477167.1"
                     /translation="MALDIYLAYVVACLLVTIVPGPTVTVIIANSLAHGTRAGLLNVA
                     GTQVGLALMMGTLIVGLTSVIEAMGWLFDWLRIAGALYLAWLGWKLIRSPDVLDTRGA
                     SAPEPRGGFFLQGVLVLLANPKALLFFGAFIPQFIDPSRGNHVAQIVILSLTAMAAAL
                     LTDGAYALLIGRAGSFLSRRRIRLVSRVSGGFLIGGGIWLALTRAR"
BASE COUNT          456 a         1012 c          852 g          485 t
ORIGIN      
        1 aggcgagcgc agcggcgttc tgcatctcgc catacgtata aatggcggag cggtactgcg
       61 tgcccgcgtc gttgccctgg cgcatgcctt gcgtcgggtt gtggccttcc cagaacacct
      121 tgagcagttg ctcgtagctg atcttcttcg gatcgaacac caccagcacc gtctcagcgt
      181 ggttggtgga gccgctgcag acctcttcgt aggtcgggtt cttcgtctcg ccgcccgagt
      241 agccgacggc ggtggaatac acgccagggg tctgccagaa gaggcgctcg gcgccccaga
      301 agcagcccat gccgaactgc gcgagctgca ggcccgccgg aaacggcgcc ttcagcggat
      361 ggccgttgac gaaatgcttc tccggcacct cgatcggcgt cgagcgtccg gcaatcgtgt
      421 tctgcggcgg cagcagggac ttcagcttct tggcaaaggc gaacatcgaa acctcctgca
      481 atagaatagc actgtgaaaa tctgcattta cggcgccggc gcggtgggcg gcctgatggc
      541 cgcctggctg acccgctcct ccggcaacga agtctccgcc gtcgcccgcg gccctcagct
      601 cgaagccctg cgcaaggacg gcctcagggt acgcgaccgc gccagcggca agatggggac
      661 gttctcgatc cttgcaagcg acgatcccgc ggtactcggg ccgcaggact acgtcatagt
      721 cgcggtgaag gcgcaaaacc ttacgcaagt cgccgccggc atcgcgccgc tgctcggccc
      781 cgacacctcg atcgtcactg cgatgaacgg cgtgccgtgg tggttcttcg actgcctcaa
      841 gtacggcggc ggcaggcagc gcctcgaatc gctcgacccc gacggcagcc tgtcgcgcgc
      901 catgccgacg cagcgcatcg tcggctgcgt ggtgcacctc gccgcgtcga cgcccgagcc
      961 cggcctgatc agccacaaca tgggcaagaa gctgatcctc ggccagcccg gcggcgcgaa
     1021 caccacgcgc gtgaagcgca tcgccaacgc cctcaccatc gccggcttcg agatcgccgt
     1081 caccggctcg atcgagaagg agttctggat caagctgctc ggcaacgtca gcttcaaccc
     1141 ggtctcggcg ctaacgcaga gcaccgccga cgtcctgatc gagaaccgcg aggtgcggga
     1201 atacatggtg gaaatcatgc gcgaggtgct ggctatcggc cgcgccgtcg gcgtcgacgc
     1261 cgccatcgac cccgaggcgc gcatcgacat ggcgcgcgtg ctgggcccgt tcaagacctc
     1321 catgctgcag gacctggagg ccggcaagcg cctcgagatc gacggtctgc tcgccggcac
     1381 cttggagatc gcgaggaagg ccggcgtcaa ggcgccctac accgagagcc tgttcggcct
     1441 gatccgcgcg cgcgccgacg ctacgaagca atatcgatga aatatggcgc actcattttg
     1501 tttattttct gtagtggcgc ttttgcgcag tattccggcc cggccgtgca ggcctgcctc
     1561 gactacgcga agcgcgagca aaagcagaac atcgtcctcg agcgcgacca gaacctgctg
     1621 atcgagcgct acacgcgcaa gctcggcagc cagttcgtgt cgtccatcct ctccggcaac
     1681 ggcgcagtgg tctacgacgg cgcgccgagc tccgagctgt cgttcgtctg cctgctggcc
     1741 gacgacaagc gggcggtgtt cttccagtgg gtggcgcgcc ccaacgcgcc ggcgaccatg
     1801 caatgcctgc gcaacccgga cctgcagaaa agtccccgcg cctgcttcga cctcatgctc
     1861 aacgtcgccg agcaggacct gaccctggtc tacgcccagc gcttccagga agccaacgag
     1921 cgcggcgaag ctcccctcgc cgccttccgc aagtcgaacg acgagtggcg gcaataccgc
     1981 gatgccgaat gcgcccgccg ccgcgacttc gcccctaccg gcgtctcccc cgacgattac
     2041 gccatcgcct gcaccgtcga gctcacccgc cgccgcgctc tggacatgag gtaaacatgg
     2101 cgctcgacat ctatctcgcc tacgtcgtcg cctgcctgct ggtaaccatc gttcccggcc
     2161 ccactgtcac ggtcatcatt gccaacagcc ttgcgcacgg cacgcgcgcc gggctcctca
     2221 acgtcgccgg gacccaagtc gggcttgcgc tgatgatggg caccctgatc gtcggcctta
     2281 cctcggtcat cgaagccatg ggctggctat tcgactggct gcgcatcgct ggcgccctgt
     2341 acctggcctg gctcggctgg aagctgatcc gctccccgga cgtcctggac acccgtggcg
     2401 cctccgcgcc tgagccgcgc ggcgggttct tcctgcaagg cgtgctcgtg ctcctcgcca
     2461 acccgaaggc tctcctcttc ttcggcgcct tcattccgca gttcatcgac ccgagccgcg
     2521 gcaaccacgt tgcccagatc gtgatcctca gcctcactgc catggccgcc gccctgctca
     2581 ccgacggcgc ctacgccctc ctcatcggcc gcgccggcag cttcctctcg cgccggcgca
     2641 tccgactcgt ctcgcgcgtc agcggcggct tcctcatcgg cggcggcatc tggctcgcgc
     2701 tgacgcgcgc ccgctgatga tctatgtcgc gcccatgatg gactggacag accggcattg
     2761 ccggtacttc ctccgtcagg tgtcttcccg ggcgcgcctc tacac
//
LOCUS       DASYJA010000257         8287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_402131, whole genome shotgun sequence.
ACCESSION   DASYJA010000257 DASYJA010000000
VERSION     DASYJA010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402131"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VGO02_05450"
     CDS             <1..622
                     /locus_tag="VGO02_05450"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEV7477168.1"
                     /translation="RSHALNELYGAQVFDEPAIQLAPTPYAKWLSCRVAMEEYHHHVR
                     FKELADQIGVQPGEMDTRNKRPLNIFELPLESWEQFCVIKMLGDLAEILQVEDLLHCS
                     FVPLRNIARTTMPEEKFHAQFGLDFCVDLVETEAGRDKVQRAVNGFYPEAPKFFGASG
                     SKNNEIYRKYGIKLRSNEDMRADYMARAKSTVEPLGITLPEISLAA"
     gene            619..1107
                     /locus_tag="VGO02_05455"
     CDS             619..1107
                     /locus_tag="VGO02_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477169.1"
                     /translation="MSAVLDGAWFSALGAPLDETERADIAAYLGGLGLPADLPVRAAS
                     TWKDAAHLCRQPAGAWWDAEEAERARLERSARLDPAQAAWLQITERLHGAAAVAAARS
                     GCADAALIRAAAGAATYAAYQQRLAQAAGAPPSHPFLRKYALYCGGHWPLGVYDQRFA
                     IF"
     gene            1118..1984
                     /locus_tag="VGO02_05460"
     CDS             1118..1984
                     /locus_tag="VGO02_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV7477170.1"
                     /translation="MKLCRFDDDRLGLVEGDAVLDVTEALAAIPPQKWPYRHGDALVA
                     HLPAVLERAKALAPRASRRKLADVRLRAPVANPSKIVNAPINYQAHIDEAKRDQGIAH
                     GRDIKTIADWGLFLKAPSSLVGPGEPIRLRFPERRNDHEVELAVVIGREANRVRREDA
                     LSYICGYSIGLDMTLRGPELPSFRKSIDTYSVLGPWLVTRDEIADPDTLDLWLTVNGE
                     ARQRSNTRRMVYNVERLIEYATSFYTLYPGDLIFSGTPEGVGPVKPGDEIRAGIQGVG
                     ELAIRVAPEYAG"
     gene            1981..3477
                     /locus_tag="VGO02_05465"
     CDS             1981..3477
                     /locus_tag="VGO02_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7477171.1"
                     /translation="MNAVEHLLGAAALARHGARTALICGDESVTYGELAARTARSAAA
                     LGTLGVRPGERVLLMMRDTPEFAAAWLGTVHAGAVAVGVNSKLSNDDARHIAIDSGAK
                     LALIEDAFVAERGRLAADVRLVGSDEWRALVRGAPQAAAFDGTPDTPALMLYSSGTTG
                     RPKGIVHRHHSILCVGASYRELDLGEGDCVFTTSKFFFAYGLEHGLLSPLSRGATSLL
                     YPDWPDAEAVVGIVSRHRPAAVFSVPTIYRRLLAEAHGRLAAFSAVRHFVAGGERLSS
                     RLELQWKKAVGAEILNLYGMSETFCACILTPPGTSDGMRTGKPLPGVEVRLLAAGGAE
                     PAAEDPGVLWVRHPAQASGYANLPEQTREQFRDGWFCSRDLFVRDADGYYIHQGRSDE
                     LIKVAGQWVQPGELEETAAREAAVAEAACVPVLDADGLERLALFVTARGDAAGAQRAA
                     LEACERTLPRHKRPKWVRAVEELPRTATGKVQRYKLREILERELAGKD"
     gene            3501..4193
                     /locus_tag="VGO02_05470"
     CDS             3501..4193
                     /locus_tag="VGO02_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV7477172.1"
                     /translation="MAYAISLWAQPALPIAGSDNTFPVRRIFCVGRNYVEHQKEMGGD
                     GREQPFFFLKAAHTLVPRGGDVHYPPKTSNYHYETEMVVAIAKGGRRIEAKKANEHIF
                     GYAVGFDMTRRDLQQTGKDHGRPWDFGKDFDEGAPCGVLAPAAKIGHPSKGAIWLNVN
                     GKQRQKADLSDMIWSVPEQIAYLSEYYTLEAGDLIYSGTPAGVGPVKPGDELHAGIDG
                     VGELKVKITPPL"
     gene            4190..4828
                     /gene="maiA"
                     /locus_tag="VGO02_05475"
     CDS             4190..4828
                     /gene="maiA"
                     /locus_tag="VGO02_05475"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEV7477173.1"
                     /translation="MKLYTYFRSSASFRVRIALNLKGIGYEASSVHLPKGEHRAAAYS
                     KVNPQALTPTLELDDGEQLAQSLAIIEYLDEVHPQPPLLPKDAKGRARVRSLSLLISC
                     EIHPLNNLRTLQYLKRALGQSEDQVNAWYRHWVADGLAKFEADLGRGGTGKFCHGDSP
                     TMADCCLVPQIFNAKRYQCDLAPYPVTMRIFDACMKLEAFDRAQPSKQPDAE"
     gene            4832..5233
                     /gene="ligA"
                     /locus_tag="VGO02_05480"
     CDS             4832..5233
                     /gene="ligA"
                     /locus_tag="VGO02_05480"
                     /EC_number="1.13.11.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018579 - protocatechuate 4,5-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 4,5-dioxygenase subunit alpha"
                     /protein_id="HEV7477174.1"
                     /translation="MGKPNAYDDIPGTTVFDAQRSRQGYHLNMFCMSLMKAENREAFK
                     ANERAYLDRWPMTEEQKQAVLDRDWNGMIDRGGNIYYLAKLFFTDQKSFQYVAAIMTG
                     STQEEYAAMMLKGGRRPEGNRYKKEWKKKRG"
     gene            5226..6068
                     /locus_tag="VGO02_05485"
     CDS             5226..6068
                     /locus_tag="VGO02_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit beta"
                     /protein_id="HEV7477175.1"
                     /translation="MARIIAGVTTSHVPAIGAALDNGKSGEPYWKKVFDGYERSKEWI
                     RKAKPDVILLVYNDHASAFSLEIIPTFALGCAAEFPIADEGWGPRPVPKVIGHPELAA
                     HIAQSVILDEFDLTVVNKMEVDHGLTVPLSLMYGQPKEWPCRVIPLPVNVVVYPPPTA
                     HRCYQFGRALRKAIRSYGKNLRVVVFGTGGMSHQLQGPRAGFINSKFDRRFLDLMTKN
                     PKALTRITHLELLREAGSEGIELVMWLIMRGALDEKVREVHRFYHVPASNTAVGHLIL
                     ENVK"
     gene            6065..7012
                     /locus_tag="VGO02_05490"
     CDS             6065..7012
                     /locus_tag="VGO02_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV7477176.1"
                     /translation="MKVCVAGQGAFGQKHLEGIASIKGIEVVSLTGGNADATKDVAQK
                     FKIPHWSTDLAESLKQPGLEAVILATPTQMHARQAIQCMRAGKHVQVEIPMAETLADS
                     REIAKVQKETGLVAMAGHTRRFNPSHQWVRKKILAGELKLQQLVVQSFFFRRTNMNAA
                     GKPRSWTDHLLWHHACHTVDLFSYQAREPIAKARALQGPMHPNLGIAMDMSIQMATPS
                     GVVCSLALSFNNDGPLGTFFRYICDNGTYIARYDDLFDGKDNKIDVSKVDVSMNGIEL
                     QDREFFAAIREKREPNASVAQCLPAMETLDRLEKSLSAP"
     gene            complement(7059..7523)
                     /locus_tag="VGO02_05495"
     CDS             complement(7059..7523)
                     /locus_tag="VGO02_05495"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEV7477177.1"
                     /translation="MDHSARHKNTNEEAFASIVGRFACQVQWGDCDPAGIIFYPTYFR
                     WMDAATWAFMDSVGYNPKRMRAEHLSMPLVSADCQFVAPALHGDRCEVRSRIARFGGA
                     SFAVTHEVIREDGATLAKGSETRVWGRYANGLGSPLKSEKIPEEVKALFRAK"
     regulatory      7570..7647
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            7694..>8287
                     /locus_tag="VGO02_05500"
     CDS             7694..>8287
                     /locus_tag="VGO02_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEV7477178.1"
                     /translation="MSDTSTLQRLFSERILLLDGAMGTMIQRARLAEEDYRGARFRDW
                     QRELRGNNDLLSLTQPRLIHDIHLQYLQAGADILETNTFNSTSIALADYGMQSLAREF
                     NRAAASLAKDAVREFAARSPATPRFVAGVLGPTNKTASISPEVNDPGYRAVSFDELVL
                     AYTEAVEGLLEGGADLLLVETVFDTLNCKAALAAIERT"
BASE COUNT         1503 a         2747 c         2723 g         1314 t
ORIGIN      
        1 gcgcagccac gcgctcaacg agctctacgg cgcccaggtc ttcgatgaac ccgcgatcca
       61 gctagcgccg acgccctacg cgaagtggtt gagctgccgc gtggcgatgg aggagtacca
      121 ccaccacgtg cgcttcaagg aactggccga ccagatcggc gtgcagccgg gcgagatgga
      181 cacgcgcaac aagcggccgc tcaacatctt cgagctgccg ctggaaagct gggagcagtt
      241 ctgcgtcatc aagatgctcg gcgacctcgc cgagatcctg caggtcgaag acctgctgca
      301 ctgctcgttc gtgccgctgc gcaacatcgc ccgcaccacc atgcccgagg agaagttcca
      361 tgcgcagttc gggctggact tctgcgtcga cctggtcgag accgaggccg gacgcgacaa
      421 ggtgcagcgc gcggtgaacg gtttctatcc ggaggcgccg aaattcttcg gcgcgagcgg
      481 ttcgaagaac aacgagatct atcgcaagta cggcatcaag ctgcgctcga acgaggacat
      541 gcgcgccgac tacatggcgc gcgcgaaaag cacggtggag cccctgggca tcacgctgcc
      601 cgaaatcagc ctcgcggcgt gagcgcggtg ctcgacgggg cctggttcag cgcgctcggc
      661 gctccactgg acgagacaga acgcgccgac atcgcggcct acctgggcgg gcttggcttg
      721 ccggccgatc tgccggtgcg ggccgcgtcg acctggaaag acgccgcgca cctgtgccgc
      781 cagccggcgg gggcctggtg ggacgccgag gaagctgaac gggcgcgcct cgagcgctcc
      841 gcccgtctgg acccggcgca agctgcgtgg ctacaaatca cggagcggtt gcacggcgcc
      901 gctgcggtgg ccgcggcgcg ctcgggttgc gccgatgcgg cgctgatccg tgccgcggcc
      961 ggcgcagcga cctatgctgc ctatcagcag cggctggctc aggcggcagg cgcgccgccc
     1021 agccacccgt tcctgcgcaa atacgcgctg tattgtggcg gtcactggcc gctcggcgtt
     1081 tacgatcaac gcttcgccat cttctaagac gaccaccatg aaactctgca gattcgatga
     1141 cgaccgcctc gggctggtcg agggcgacgc ggttctggac gtgaccgagg cgctggccgc
     1201 gatcccgccg cagaagtggc cttatcggca cggcgatgcc ctggtggcgc atctgccggc
     1261 ggtgctggag cgtgcgaagg cgctcgcgcc gcgcgcctcg cgccgcaagc tcgccgatgt
     1321 gcgcctgcgc gcgccggtcg cgaacccgtc gaagatcgtc aacgcgccga tcaactacca
     1381 ggcgcatatc gacgaggcga aacgcgacca gggcatcgcg catggacgcg acatcaagac
     1441 catcgcggac tggggcctgt tcctcaaagc accgagctcg ctcgtcggcc cgggcgagcc
     1501 gatccgcctg cgcttcccgg agcgacgcaa cgaccacgag gtcgagctgg cggtggtgat
     1561 cgggcgcgag gcgaatcgcg tgcgccggga agacgcgctt tcctacattt gtggctactc
     1621 catcgggctc gacatgacgc tgcgcggccc ggagctgccg agctttcgca agtcgatcga
     1681 cacctacagc gtcctcggcc cgtggctggt gacgcgcgac gagatcgccg acccggacac
     1741 gctcgatctg tggctgacgg tcaacggcga agcgcggcaa agatcgaata cgcgccgcat
     1801 ggtctacaac gtagagcgcc tgatcgaata cgcgacgagc ttctacacgc tgtacccggg
     1861 cgacctgatc ttcagcggca cgccggaagg cgtcggcccc gtgaagccgg gcgacgagat
     1921 ccgcgccggc atccagggtg tgggcgagct cgcgatccgc gtcgcgcccg aatacgccgg
     1981 atgaacgcgg tcgaacacct gctcggcgcc gcggcgctcg cgcggcacgg cgcccggaca
     2041 gcgctcatct gcggagacga gagcgtcacg tatggcgagc tcgcggcgcg cacggcgcgc
     2101 tcggcggcgg cgctgggcac gctcggcgtg cggcccggcg agcgcgtcct gctgatgatg
     2161 cgcgatacgc ctgagtttgc cgccgcgtgg ctgggtaccg tgcacgccgg cgcggttgcg
     2221 gtcggcgtga acagcaagct ctccaacgac gatgcgcgcc acatcgcaat cgacagcggc
     2281 gcgaagcttg cgctgatcga ggatgcgttc gtagccgagc gcggcaggct cgccgcagat
     2341 gtgcggctgg tcggctcgga cgagtggcgt gcgctggtgc gcggcgcccc gcaggccgcc
     2401 gcgttcgacg gcacgcccga tacgcccgcg ttgatgctgt actcgtccgg caccaccggc
     2461 cgcccgaagg gcatcgtgca ccgtcaccac agcatcctct gtgtcggcgc gtcgtatcgc
     2521 gagctcgacc tgggtgaggg cgactgcgtc ttcaccacct caaagttctt tttcgcctat
     2581 ggcctcgagc acggcctgct ctccccgctg tcgcgcggcg ctacgtcgct cctgtacccg
     2641 gattggcccg acgccgaagc cgtagtcggc attgtttccc ggcaccgccc ggcggccgtg
     2701 ttcagcgtgc cgacgatcta tcgccggctc ctggccgaag ctcatggccg gctcgccgct
     2761 ttctccgccg tgcgccactt cgtcgccggc ggcgagcgcc tgtcttcgcg gctcgagctg
     2821 caatggaaaa aggcggtggg cgccgagatt ctcaacctgt acggcatgtc ggagactttc
     2881 tgcgcgtgca tcttgacgcc accaggcact tcagacggca tgcgcaccgg caagccgctt
     2941 cccggcgtcg aggtgcggct gctcgcggcc ggcggcgccg agcctgctgc cgaggatccg
     3001 ggcgtgctct gggtgcgtca tcccgctcag gcgagcggct acgccaacct gcccgagcaa
     3061 acgcgcgagc aattccgcga cggctggttc tgcagccgcg accttttcgt gcgcgatgca
     3121 gacggctatt acatccacca gggacgctcg gacgagctca tcaaagtcgc cgggcagtgg
     3181 gtgcagcccg gcgagctcga ggagactgcc gcgcgcgagg ccgcggtagc cgaggcggct
     3241 tgcgtcccgg tgctggacgc cgacgggctg gagcgcctgg cattgttcgt cactgcccgc
     3301 ggcgacgcgg cgggggcgca gcgcgccgcg ctcgaggcct gcgagcggac actgccgcgc
     3361 cacaagcggc cgaagtgggt gcgcgcggtg gaagagctgc cgcgcaccgc taccggcaag
     3421 gtgcaacgct acaagctgcg cgaaatcctg gagcgcgagc tcgcgggcaa ggactagaat
     3481 cccccttttt agaggaccgc atggcctacg cgatttccct gtgggcccag ccggcgctgc
     3541 cgattgctgg gtcggacaat accttcccgg tgcgacgcat cttctgcgtc ggccgcaact
     3601 acgtcgagca ccagaaggaa atgggcggcg acggccgcga gcagccgttc ttcttcctga
     3661 aggcggcgca cacgctggtg ccgcgcggcg gcgacgtgca ctacccgccg aagacctcca
     3721 attaccatta cgaaaccgag atggtggtgg cgatcgccaa gggcggccgg cgcatcgaag
     3781 ccaagaaagc gaatgagcac atcttcggct acgccgtcgg cttcgacatg acgcgccgcg
     3841 atctgcagca gaccggcaag gaccacgggc ggccctggga cttcggcaag gatttcgacg
     3901 aaggcgcgcc ctgcggcgtc ctcgcgccgg ccgcgaagat cggccatccg tccaaaggcg
     3961 cgatctggct caacgtcaac ggcaagcagc gccagaaagc cgacttgtcg gacatgatct
     4021 ggtcggtgcc cgagcagatt gcgtatctct ccgagtacta cacgctcgag gcgggcgacc
     4081 tgatctactc gggaaccccg gcgggcgtcg gcccggtgaa gccgggcgac gagctgcacg
     4141 caggcatcga cggcgtcggc gagctgaagg tgaaaatcac cccgccgctg tgaagctgta
     4201 cacgtacttc cgcagctcgg cgtcgtttcg cgtgcgcatt gcgctcaacc tcaagggcat
     4261 cggctacgag gcaagctccg tgcatctgcc gaagggcgag caccgtgcgg cggcctattc
     4321 gaaggtcaat ccgcaggcgc ttaccccgac cctggaactc gacgacggcg agcagctcgc
     4381 gcagtcgctc gccatcatcg agtaccttga cgaggtccac ccccagccgc cgctcctgcc
     4441 gaaggatgcg aaggggcgcg cgcgcgtacg ctcgctgtca ctgctcatct cctgcgagat
     4501 ccatccgttg aacaacctgc gcacgctgca gtacctcaag cgcgcgctgg gtcagagcga
     4561 ggaccaggtg aacgcctggt accggcactg ggtggccgac ggactcgcca agttcgaggc
     4621 agatctcggt cgcggcggca ccggcaagtt ctgccatggg gactctccca ccatggccga
     4681 ttgctgcctg gtgccgcaga tcttcaacgc caagcgctac cagtgcgacc tggcgccgta
     4741 tcccgttacc atgcgcatat tcgacgcgtg catgaagctc gaagccttcg atcgcgcgca
     4801 gccttcgaag caaccggacg ccgagtaagc catgggcaag ccgaacgcat acgacgacat
     4861 cccgggcacc acggtgttcg acgcgcagcg ctcgcgccag ggctaccacc tgaacatgtt
     4921 ctgcatgtcg ctgatgaagg ctgagaaccg cgaagcgttc aaagctaacg agcgcgccta
     4981 cctcgaccgc tggccgatga ccgaggagca gaagcaagcg gtgctcgatc gcgactggaa
     5041 cggcatgatc gatcggggag ggaacatcta ctatctcgcc aagctgttct tcaccgacca
     5101 aaagtcgttc cagtacgtgg ccgcgatcat gaccggcagc acgcaggagg agtacgccgc
     5161 gatgatgctg aagggcggcc ggcggccgga aggcaaccgc tacaagaaag agtggaaaaa
     5221 gaagcgtggc tagaatcatc gccggagtga ctacctcgca cgtgccggcg atcggcgcag
     5281 cgctcgacaa cggcaagagc ggcgagccct actggaaaaa ggtgttcgac ggctacgagc
     5341 gctccaagga atggatccgc aaggcgaagc ccgacgtgat cctgctggtg tacaacgatc
     5401 acgcctcggc gttctcgctg gaaatcatcc cgaccttcgc gctcggctgc gctgcggagt
     5461 tcccgatcgc ggacgagggc tgggggccgc gcccggtgcc gaaggtgatc ggccatcccg
     5521 aactcgcggc gcatatcgcg caatcggtga tcctcgacga gttcgatctc accgtggtca
     5581 acaagatgga ggtcgaccat gggctgacgg tgccgctgtc gctgatgtat gggcagccga
     5641 aagagtggcc ctgccgggtg atcccgctgc cggtgaacgt ggtggtctac ccgccgccga
     5701 cggcgcaccg ctgctaccag ttcgggcgcg cgctgcgcaa ggcaatccgt tcgtacggca
     5761 agaatctgcg tgtggtggta ttcggcaccg gcggcatgtc gcaccagttg caggggccgc
     5821 gcgcggggtt catcaacagc aagttcgacc gccgcttcct ggatctcatg accaagaacc
     5881 cgaaggcgct cacgcgcatc acgcatctcg agctgctgcg cgaagcgggc tcggaaggca
     5941 tcgagctggt gatgtggctg ataatgcgtg gcgcactcga cgagaaggtg cgggaagtgc
     6001 accgcttcta ccacgtgccc gcctcgaaca ctgcggtcgg gcaccttatc ctggagaacg
     6061 tcaaatgaag gtttgcgtag cgggtcaggg tgccttcggg cagaagcacc tcgagggcat
     6121 agccagcatc aagggaatcg aggtggtctc gctcacgggc ggcaacgccg atgcaacgaa
     6181 ggatgtggcg cagaagttca agatccccca ctggagcacc gacctcgccg agagcctgaa
     6241 gcagccaggc ctggaggcgg tgatcctcgc caccccgacg cagatgcacg cgcgccaggc
     6301 gatccagtgc atgcgggccg gcaagcacgt gcaggtcgag atcccgatgg ccgagaccct
     6361 ggccgactca cgcgagatcg cgaaggtgca gaaagagacc ggcctggtcg cgatggcggg
     6421 gcatacgcgc cgcttcaacc ccagccacca gtgggtgcgg aagaaaatcc tcgccggcga
     6481 gctcaagttg cagcagctcg tggtgcaatc gttcttcttc cgccgcacca acatgaacgc
     6541 cgcaggcaag ccgcgcagct ggactgatca cctgctgtgg catcacgcct gccacacggt
     6601 tgacctgttc tcctaccagg cgcgcgagcc gatcgccaaa gctcgcgcgc tgcaggggcc
     6661 gatgcatccg aacctcggca tcgccatgga catgagcatc caaatggcca cgccgtcggg
     6721 cgtggtgtgc tcgctcgcgc tttcgttcaa caacgacggg ccgctcggga cgttcttccg
     6781 ctacatctgc gacaacggca cttacatcgc gcgctacgac gatctgttcg acggcaagga
     6841 caacaagatc gacgtatcga aagtcgatgt ctccatgaac ggcatcgagc tgcaggaccg
     6901 cgagttcttc gccgcgatcc gcgagaagcg cgagcctaat gcgagcgtcg cgcagtgcct
     6961 gccggcgatg gaaacgctcg accgtctgga gaagagcctg tctgctcctt gaacgggttc
     7021 acgcactgcc gccgacgcca tgatcgatcg cgtgcttgct acttggcgcg gaacagcgcc
     7081 ttgacctctt cgggaatctt ctcgcttttc agcggcgagc ccaggccgtt cgcatagcgg
     7141 ccccagacgc gcgtctcgct gcccttggcc agcgtggcgc catcctcgcg aataacctcg
     7201 tgcgtcactg cgaaagatgc gccgccgaag cgcgcgatgc gcgagcgcac ctcgcagcga
     7261 tcgccatgca gggcgggcgc cacgaactgg cagtcggccg acaccagcgg catcgataga
     7321 tgctccgcac gcatgcgctt cgggttatag cccaccgaat ccatgaaggc ccaggtcgcc
     7381 gcgtccatcc agcggaaata ggtcggatag aaaatgatgc cggctggatc gcaatcgccc
     7441 cattgcacct ggcacgcaaa gcgcccgacg atggacgcaa aggcctcttc gttcgtgttt
     7501 ttgtgacgcg ccgaatgatc cattcgaacg attataagcg gttgacacta caggggttga
     7561 tgggcagaat cccaccctcg cgccgatttg atgttcagct tgcgggcgcg ttacaaatgc
     7621 cgctaaagag acgcgcgaag gaagcccgtt tctgcaacaa gctgaacggg ctttttcttt
     7681 ttcgcggagc gtcgtgagcg atacgagcac cctgcagcgc ctcttctctg agcggatcct
     7741 cctgctggac ggcgccatgg gcacgatgat tcagcgcgca cggctcgccg aagaggacta
     7801 tcgcggcgcg cgctttcgcg actggcagcg ggagttgcgc ggcaacaacg atctgctgtc
     7861 gcttacccag ccgcggctga tccacgacat ccatctgcag tatctgcagg cgggcgccga
     7921 catcctcgaa accaacactt tcaattcgac ttccatcgcg ctcgccgact acggcatgca
     7981 atccctggcg cgcgagttca accgggcggc ggcgagcctg gcgaaggacg cggtgcgcga
     8041 gttcgcggcg cgcagtccgg caacaccacg cttcgtcgcc ggcgtgctcg gcccgacgaa
     8101 caagaccgcc tcgatctccc cagaagtgaa cgacccgggc tatcgcgccg tcagcttcga
     8161 cgagctggtg ctcgcctaca cagaagcagt cgagggcttg ctcgagggcg gcgccgatct
     8221 cctgctggtc gaaaccgtgt tcgacacgct caactgcaag gcggcccttg cagcgatcga
     8281 gcgcaca
//
LOCUS       DASYJA010000258         5458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1038649, whole genome shotgun sequence.
ACCESSION   DASYJA010000258 DASYJA010000000
VERSION     DASYJA010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038649"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(336..1736)
                     /locus_tag="VGO02_05505"
     CDS             complement(336..1736)
                     /locus_tag="VGO02_05505"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEV7477179.1"
                     /translation="MKPAFHERTAADLAAAFSRRELTPMEATRGALARIAEMEPSLNA
                     MYRVDREGAIEQSRAAEARWEAGRPLSPLDGVPLTIKENVYTRGAPAPIGTRANEDAP
                     PQPEDAPPAARLREAGCVILGKTTMPDYGMLSSGLSSLHGVTRNPWRRDRNPSGSSSG
                     AGAACAAGYAPLHLGTDIGGSVRLPATHCGIFALKPSLGRVPIHPPYMGRVAGPMTRT
                     VEDAALMLNILIRPDGRDYMALPQQEQDFTWGLDDIDPRRIKIGLLPDMGVGLSIDHE
                     VRAATEAAATALANAGCNVESIRSFLTEEMLDGVCRFFEARSYNDYMLLPAARRQKVL
                     PFIVEWCSWRAGKFSGRDVMQAYNQVLSMRDAAVGASREYDFLLSPTSPILPYEAELP
                     APGNDSHNALPHIAFTVPFNMSEQPAASINWSYSSDGLPIGVQIVGQRFDDTGVLRLA
                     RLVERIRPTQRRPTAT"
     gene            1763..3082
                     /gene="ugpB"
                     /locus_tag="VGO02_05510"
     CDS             1763..3082
                     /gene="ugpB"
                     /locus_tag="VGO02_05510"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HEV7477180.1"
                     /translation="MLRCAIVGQVIVACVLAFACAPAWAQWEIRVWHAMSGTQGVEFD
                     SLVERFNAAQQDYRVVSAYKGSYAETLAALLDTRRRGSRSVPPHVVQIHETGTAEILA
                     RRGLTMPLWQILQDADEKLEGYLPPVEAPFTDADGRLLALPISAATPVLYYNRDALRA
                     AKLEPPKTWYEMPAVLAALSKAGYGCPYTTAFPSWVLLENMSAWHDQRFATQRNGMGG
                     ADARLAFNSRLMVRWISMLSTWREAGYFVYWGRGDEAEARFAADHCAMLTSSSASYAA
                     LRARAKFDLGVAQLPYYDDFDDAPQNTLAATAGLWVLAGKPPVEYLGVARFFAFLAQP
                     GVQAEWTQKTGELPLTYAAYELMRKQGFYAANPGQEIAVQQVLAKLPTWDSRGIRLGQ
                     FPRIRGIINEELEQVWGGKKSALDALNAAVARGNVLLEELEKTESGK"
     gene            3090..3548
                     /locus_tag="VGO02_05515"
     CDS             3090..3548
                     /locus_tag="VGO02_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013345880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HEV7477181.1"
                     /translation="MSPRLLRWGMNLWPPFRGAGIRVRHIADDWSEARVELRAGRLRR
                     NFVGTHYGGSLFSMTDPFYALMLIHRLGERYLVWDQAASIDFIAPGRGKVAAVFSLNE
                     QQVQDIRGRAAGGEKVLPQFDVEVKDEAGALVARVRKTLYVRLKPRYRPQ"
     gene            complement(3549..3869)
                     /locus_tag="VGO02_05520"
     CDS             complement(3549..3869)
                     /locus_tag="VGO02_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477182.1"
                     /translation="MKNFAKLVAAAAIATVWLPSQAQDTRRIDQREANQQKRIEQGEK
                     SGALTQKETKRLQKGQAHVQKMETKAEADGKVTKQERAKIEHAQNVQSKKIYREKHDK
                     QTAK"
     gene            complement(3954..4103)
                     /locus_tag="VGO02_05525"
     CDS             complement(3954..4103)
                     /locus_tag="VGO02_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477183.1"
                     /translation="MDDQSNKALKDLTKLIAAAAIATVWLPAYPLTDKRPEEQQASQV
                     AKPQQ"
     gene            complement(4140..4637)
                     /locus_tag="VGO02_05530"
     CDS             complement(4140..4637)
                     /locus_tag="VGO02_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEV7477184.1"
                     /translation="MIGTQAMASCPSLLDRSMDTLLEKPQSLCEYSGKVVLVVNTASQ
                     CGYTPQYDGLEALYKKYRPRGLVVLGFPMNDFGGQEPGSNKEISQFCVNQYAIDFPMF
                     AKTDLKKNPLYVDLVKATGQSPKWNFHKYLVDRSGQKVQSFETKVAPEDPKLVAAIER
                     LLDAK"
     gene            complement(4648..>5458)
                     /locus_tag="VGO02_05535"
     CDS             complement(4648..>5458)
                     /locus_tag="VGO02_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEV7477185.1"
                     /translation="LGCEVEELYCEVDGRFPNHHPDPSVPKNLAELIQTVKDGKSELG
                     IAFDGDGDRLGLVTKDGEIIFADRQLMLLAKDVLSRNPGAQIIYDVKSTRLLEPWIAR
                     HGGRPLIWKTGHSLIKAKLKETGALLAGEMSGHTFFKERWYGFDDALYGGARLLEILS
                     READPNRTLKSLPNAPSTPELHWSLAEGEPHALVAKLQASKPFPGAERVLTIDGVRVE
                     YADGFGLARASNTTPVIVLRFEADTAAALERIKKDFRAALQPLKPNAPLPY"
BASE COUNT          812 a         1796 c         1877 g          973 t
ORIGIN      
        1 gcgtacgttt catggcaggg ccaaacaaag ggcagtcgtc agtttgatcg ccgcacctgg
       61 tatctgaggg caatcccgga gggcacagtt agtcgctgtg ccctccgcta aaacggccag
      121 cacgctgtta gaagcccccg cctcgagccg tacgttgcat ggcagggcca aacaaagcgc
      181 agtcgtcagt ttgatcccgg agggcacagc aggcagctgt gcccttcgtc tttctaccgc
      241 cgcgaaacca gtttgccgca ggcccgcacc gctcgctatc gccgcgcacg gctaaaacgt
      301 gtcgcgtttc tccgcgccgt gcgctcgtcg atcgtctacg tcgccgttgg gcgtcgttgc
      361 gtcgggcgga tcctctcaac cagcctcgcc aggcgcagca cgcccgtgtc gtcgaagcgc
      421 tgaccgacga tctgcacgcc gatcggcagc ccgtcgctgc tgtagctcca gttgatcgac
      481 gccgcaggct gttccgacat gttgaagggc acggtgaacg cgatgtgcgg cagcgcgttg
      541 tgcgaatcgt tgcccggcgc cggcagctcg gcctcgtagg gcaggatcgg cgaggtcggc
      601 gaaagcagga agtcgtactc gcgcgaggcg ccgaccgcgg cgtcgcgcat cgacaggacc
      661 tggttgtatg cctgcatcac gtcgcggccg gagaacttgc cggcgcgcca cgagcaccac
      721 tcgacgatga agggcaggac cttctggcgc cgcgccgcag gcagcagcat gtagtcgttg
      781 tacgagcgcg cctcgaagaa gcggcacacg ccgtcgagca tctcctcggt gaggaatgag
      841 cggatcgact cgacgttgca gccggcgttg gcgagcgccg tggcggcggc ctcggtggca
      901 gcgcgcacct catggtcgat cgacaggccg acccccatgt cgggcagcaa gccgatcttg
      961 atcctgcgcg ggtcgatgtc gtcgaggccc caggtgaagt cctgctcctg ctgcggcagc
     1021 gccatgtagt cgcggccgtc ggggcggatc aggatgttca gcatcagtgc cgcgtcctcg
     1081 accgtgcgcg tcatcggccc ggccacgcgg cccatgtagg gcgggtggat cggcacgcgg
     1141 ccgagcgacg gcttgagggc aaagatgccg cagtgggtcg ccggcaggcg caccgagccg
     1201 ccgatgtcgg tgcccaggtg cagcggcgcg tagccggcgg cgcaggcggc gccggccccc
     1261 gagctcgagc ccgacgggtt gcggtcccgg cgccatgggt tgcgcgtcac gccgtgcagg
     1321 ctggacaggc ccgaggacag catgccgtag tcgggcatgg tggtcttgcc gaggatcacg
     1381 cagcccgcct cgcgcaggcg cgcggccggc ggcgcatcct ccggctgcgg cggcgcgtcc
     1441 tcgttggcgc gcgtgccgat gggcgcgggc gcgccgcgcg tatagacgtt ttccttgatg
     1501 gtgagcggca cgccgtccag cggcgagagc ggccggccgg cttcccagcg cgcctcggcg
     1561 gcgcgcgact gctcgatcgc gccctcgcgg tcgacccggt acatggcatt gagactcggc
     1621 tccatctcgg cgatgcgcgc cagcgcgccg cgcgtcgcct ccatcggcgt gagctcgcgg
     1681 cggctgaagg cggccgccag gtcggcggcg gtgcgctcat ggaaagcggg cttcatgggc
     1741 cgactatata ctgcgcgcca ccatgctgcg ctgcgcgatc gtcgggcagg taatcgttgc
     1801 ttgtgtgctc gccttcgcgt gcgcgccggc gtgggcgcag tgggagatcc gggtctggca
     1861 cgcgatgagc ggcacgcaag gcgtggagtt cgacagcctg gtcgagcgct tcaacgccgc
     1921 gcagcaggac taccgcgtgg tgtccgccta caaaggctcc tacgccgaga cgctcgcagc
     1981 gctgctcgac acgcggcgca gggggtcgcg cagtgtgccg cctcacgtcg tccagattca
     2041 cgagaccggc accgcggaga tactggcgcg gcgcggcctg accatgccgc tgtggcagat
     2101 cctgcaggac gccgacgaga agctcgaggg ctacctgccg ccggtggagg cgccgttcac
     2161 cgacgccgac ggccgtctgc tcgctctgcc gatcagcgcc gccaccccgg tgctctatta
     2221 caaccgcgac gccctgcgcg cggcgaagct cgagccgccg aagacgtggt acgagatgcc
     2281 ggcagtcctc gccgcgctgt ccaaggcggg atacggctgc ccgtatacga ccgcgtttcc
     2341 ctcctgggtg ctgctcgaga acatgagcgc ctggcacgac cagcgcttcg ccacgcagag
     2401 gaacggcatg ggcggcgcgg acgcccgtct cgccttcaac agccggctga tggtgcgctg
     2461 gatctcgatg ctctcgacct ggcgcgaggc gggctacttc gtgtactggg ggcgcggcga
     2521 cgaggccgag gcgcgcttcg ccgccgatca ctgcgccatg ctcacctcgt cgtcggccag
     2581 ctatgccgcg ctgcgcgcgc gcgccaagtt cgacctcggc gtggcgcagc tgccctacta
     2641 cgacgatttc gacgacgcgc cgcagaatac gctcgccgcg acggcgggac tgtgggtcct
     2701 cgccggcaag ccgccggtcg agtacctggg tgtggcgcgc ttcttcgcct tccttgcgca
     2761 gccgggcgtg caggcggaat ggacccagaa gaccggggag ctgccgctca cctatgccgc
     2821 ctacgagctg atgcgcaagc agggcttcta cgccgccaat cccggccagg agatcgccgt
     2881 gcagcaggtg ctcgccaagc tgccgacctg ggactcgcgc ggcatccgcc tgggccagtt
     2941 cccgcgcatc cgcggcatca tcaacgagga gctcgagcag gtctggggcg gcaagaagtc
     3001 ggcgctcgac gccctcaacg ccgcggtggc gcgcggcaac gtgctgctcg aggagctcga
     3061 gaagaccgag tcgggaaaat gagcgggcga tgagcccgcg cctgctgcgc tgggggatga
     3121 acctctggcc gccgttccgc ggcgccggca tccgcgtgcg ccacatcgcc gacgactgga
     3181 gcgaggcgcg cgtcgagctg cgcgccgggc ggctgaggcg caacttcgtc ggcacgcatt
     3241 acggcggcag cctgttctcg atgaccgatc cgttctacgc gctgatgctg atccaccgcc
     3301 tcggtgagcg ttacctggtg tgggaccagg cggcgagcat cgacttcatc gcgccgggtc
     3361 gtgggaaagt cgctgccgtg ttctctttga atgaacaaca agtgcaagac atccgcggcc
     3421 gggcggccgg cggcgagaag gtactgccgc agttcgacgt cgaggtgaag gacgaagcgg
     3481 gcgcactggt cgcccgcgtt cgcaagactc tgtatgtcag gctgaagccg cgctaccggc
     3541 cgcagtaact acttcgcggt ctgcttgtcg tgcttctcgc ggtagatctt cttgctctgc
     3601 acgttctgcg cgtgctcgat cttcgcgcgc tcctgcttcg ttaccttgcc gtcggcctcg
     3661 gccttggtct ccatcttctg cacgtgcgcc tgtcccttct ggagccgctt cgtctccttc
     3721 tgcgtcagcg cgccggattt ctcgccttgc tcgatgcgct tctgctggtt ggcctcgcgc
     3781 tggtcgatgc ggcgcgtgtc ctgcgcctgc gacggtagcc acacggtggc gatggctgcg
     3841 gcggcgacga gttttgcgaa gtttttcacg gttctgcctc cttggtggtt gagcgacagg
     3901 cagaacaacg tcgccgcaag gggggcggtt taccgcggat ttgttaccaa tcgttactgc
     3961 tggggcttcg cgacctgcga tgcctgctgt tcttccggcc gcttgtcggt gagcgggtag
     4021 gccggcagcc agaccgtggc gatcgccgcc gcggcgatca gcttggtcaa gtccttcagc
     4081 gccttgttgc tttggtcatc catggatcaa ggtgtatcac gcctttgtta cgttttcact
     4141 catttggcgt cgaggagtct ctcgatggcc gcgaccagct tcggatcctc aggcgcgact
     4201 ttcgtctcga agctctgcac tttctgtccc gaacggtcga ccaggtactt gtggaagttc
     4261 cacttgggcg actggccggt ggctttcacc aagtcgacgt acaggggatt ctttttcaga
     4321 tccgtcttgg cgaacatcgg gaaatcgatg gcgtactggt tgacgcagaa ctgcgagatc
     4381 tccttgttcg agccgggctc ctggccgccg aagtcgttca tcgggaagcc gagcaccacc
     4441 aggccgcgcg gccggtactt cttgtagagg gcttccagcc cgtcgtactg cggcgtgtag
     4501 ccgcactggc tggcggtgtt gacgaccagc accacctttc ccgagtactc gcacagcgac
     4561 tgcggcttct cgaggagcgt gtccatgctg cgatccagca ggctcgggca agaggccatg
     4621 gcttgggtgc cgatcaagaa caaaatacta gtaaggaagg ggcgcattcg gtttcagcgg
     4681 ctgcagcgcg gcccggaaat ccttcttgat gcgttccagc gcggcagccg tatccgcttc
     4741 aaagcgtagc acgatcaccg gcgtggtgtt cgaggcgcgc gccaggccga agccgtcggc
     4801 gtattccacg cgcacgccgt cgatcgtcag cacgcgctcg gcgcccggga aaggcttgga
     4861 cgcctgcagc ttggcgacca gggcatgcgg ctcgccctcg gcgaggctcc agtgcagctc
     4921 gggcgtggag ggcgcgttgg gcagggattt cagggtgcgg ttcggatcgg cttcccggga
     4981 caggatctcg agcagccgcg cgccgccgta aagcgcgtcg tcgaagccgt accagcgctc
     5041 cttgaagaag gtatggcccg acatctcgcc cgcgagaagc gcgccggtct ccttcagctt
     5101 cgccttgatc agcgagtggc cggtcttcca gatgagcggc ctgccgccgt ggcgcgcgat
     5161 ccacggctcg agcaggcgcg tcgacttcac gtcgtagatg atctgcgcgc cgggattgcg
     5221 cgagagcacg tccttcgcca gcagcatcag ctggcggtcg gcgaagatga tctcgccgtc
     5281 cttggtgacc aggccgaggc ggtcgccgtc gccgtcgaac gcgatgccga gctcgctctt
     5341 gccgtccttc acggtctgga tcagctcggc gagatttttc ggcaccgacg gatcgggatg
     5401 gtggttcgga aagcgcccgt ccacctcgca gtagagttcc tcgacctcgc agccgagg
//
LOCUS       DASYJA010000259         1572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1946329, whole genome shotgun sequence.
ACCESSION   DASYJA010000259 DASYJA010000000
VERSION     DASYJA010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1946329"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VGO02_05540"
     CDS             <1..630
                     /locus_tag="VGO02_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEV7477186.1"
                     /translation="PYDFAQHGFAMNVYIADPVEWVALFKMPNSGPPGLWRIVFPTAD
                     RDDAATLSAENVERRMQGFIARRASYEVRYKSVYRVHQRVCKTFRSGRVVLAGDAAHV
                     NNPLGAFGLNSGIHDAISIAEKLGRVWRSEADDSLLDLYVRQRRMAAVEQVERMSIRN
                     KRQLEERDPAVQRRRLQELMAIAQDPVRARDHLLETSMITGLRQSGQIR"
     gene            714..1487
                     /locus_tag="VGO02_05545"
     CDS             714..1487
                     /locus_tag="VGO02_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEV7477187.1"
                     /translation="MNKYTAVATAALASVAGNALAENQLEHCNETLGTLAVIEDRNAS
                     WYYHMQQYKVQSTVPLLRMLIQKSNCFVVVERGQAMNSMKQERNLDSSGETRQGSNFG
                     KGQMVAADYALNPTIDFSARDTGGISGAIGGLTSKLGSIGQAVGGVAGNLKFREASTM
                     LTLVDNRSGVQLAAAQGSSSKTDFGAWGGVFGGGGAVAGGGYTNTPEGKVIASAFADA
                     YNNLVKAAKNYKAQEVRGGLGTGGRLGVQGGSTPASKGQ"
BASE COUNT          299 a          505 c          517 g          251 t
ORIGIN      
        1 ccgtacgact tcgcgcagca cggcttcgcg atgaacgtct acatcgccga cccggtggag
       61 tgggtggcgc ttttcaagat gccgaattcc ggcccgcccg gcctgtggcg catcgttttc
      121 cccaccgccg accgggacga cgccgcgacc ctctccgccg agaatgtcga gcgccggatg
      181 cagggcttca tcgcgcgccg cgcatcctac gaggtgcgct acaagagcgt ctaccgcgtt
      241 caccagcgcg tgtgcaagac cttccgctcc ggacgcgttg tgctcgccgg cgacgccgcc
      301 cacgtcaaca acccgctggg cgccttcggc ctgaacagcg ggatccacga cgccatcagc
      361 atcgccgaga agctcggccg cgtctggcgc agcgaagccg acgactcgct gctcgatctc
      421 tacgtgcgcc agcggcgcat ggcggcagtc gagcaggtgg agagaatgtc gatccgcaac
      481 aagcgccagc tcgaggagcg cgacccggcg gtgcagcgca ggcggctgca ggaactgatg
      541 gcgatcgcgc aggacccggt gcgcgcgcgc gatcaccttc tcgagacctc gatgatcacc
      601 ggcctgcgcc agagcggcca gatccgctag cgcgctgttg cctctagatc cttcttccca
      661 aaggcttttt gttctatagt gtcagggcgg caaatacggg aggggtcctg atcatgaata
      721 aatacactgc agtagccacg gccgcgcttg cgtccgtcgc cggcaacgca ctggcggaaa
      781 accagcttga gcattgcaac gagacgctcg gcacgctcgc cgtgatcgag gatcgcaacg
      841 ccagctggta ctaccacatg cagcaataca aggtgcagtc cacggtgccg ctgcttcgca
      901 tgctgatcca gaagtcgaac tgtttcgtgg tggtggagcg cgggcaggcg atgaattcga
      961 tgaagcagga gcgcaacctc gacagctcgg gtgagacgcg ccagggcagc aacttcggca
     1021 agggccagat ggtggcggcc gactatgccc tgaacccgac catcgatttc agcgcccgcg
     1081 acaccggcgg catcagcggc gcgatcggtg gcctcaccag caagctcggc tcgatcgggc
     1141 aagccgtggg cggagtggcc ggtaatctta agttccgcga ggcctcgacc atgctgacgc
     1201 tggtcgacaa ccgctcaggt gtgcagctag ccgccgcgca aggcagctcg tcgaagaccg
     1261 atttcggtgc ctggggcggg gtgttcggcg gcggcggcgc tgtggccggc ggcggctaca
     1321 ccaacacgcc cgaaggcaag gtgatcgcca gcgctttcgc cgatgcgtac aacaacctgg
     1381 tgaaagcggc caagaactat aaggcgcagg aagtgagggg cggcctggga acgggcggac
     1441 gcctcggcgt gcagggcggc agcaccccgg cttcgaaggg gcaatgaaca agtaggttgc
     1501 cggtccgcgc catggtgctg tatattcata cagtaccatg gcggctctcg aagacatcct
     1561 ccagcagcat tc
//
LOCUS       DASYJA010000260         5372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2126863, whole genome shotgun sequence.
ACCESSION   DASYJA010000260 DASYJA010000000
VERSION     DASYJA010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2126863"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VGO02_05550"
     CDS             complement(<1..308)
                     /locus_tag="VGO02_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEV7477188.1"
                     /translation="MSSINLTHHFLIAMPSMADPHFAHTLIYICEHNENGALGIVINK
                     PIDMTLSALFEQINVPLGDSELREARVHYGGPVQTDRGFVLHEPAGNWQSTLRVRDAI
                     G"
     gene            complement(370..555)
                     /locus_tag="VGO02_05555"
     CDS             complement(370..555)
                     /locus_tag="VGO02_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEV7477189.1"
                     /translation="MSDTQVQFKTWMCLICGFLYDEAAGLPDEGIAPGTRWEDVPMNW
                     TCPECGARKDDFEMVEI"
     gene            608..1366
                     /locus_tag="VGO02_05560"
     CDS             608..1366
                     /locus_tag="VGO02_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEV7477190.1"
                     /translation="MAKSPPIVLTFAASDPTGGAGLQADVLTLAARGCHPLSVVTALT
                     VQDTRGVESLHAVEAARVTEQAHCLLADFKVAAFKLGVLGSASNTAVIARLLAAHRHA
                     PVVVDPVLASGRGDALAGEDSISTMQQAIFPLTAVATPNTLEAQRLGGISAMLAAGCR
                     YVLVTGTHEEGTDVVNRLHDSRGLVREDRWPRLAGSYHGSGCTLASALAAALAKGMAM
                     PEAVRDAQDYTWHALAAGFRPGAGQFIPQRFFSQ"
     gene            1363..1947
                     /gene="thiE"
                     /locus_tag="VGO02_05565"
     CDS             1363..1947
                     /gene="thiE"
                     /locus_tag="VGO02_05565"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV7477191.1"
                     /translation="MKLRGLYAITPEGAGVVDKARQALEGGIALLQYRQKNRTSLEEA
                     GALLALARRHGVPFIVNDDVELALAIGADGVHLGGEDGDLAAARSKLKGKLLGASCYN
                     RPELAQAALAAGADYIAFGSVFSSPTKPGAVRAPLSLFENKLSKPVCAIGGITLQNAA
                     QVIAAGADMVAVISDLFDAPDIARRAAAYRKLFP"
     gene            1944..3215
                     /gene="hemL"
                     /locus_tag="VGO02_05570"
     CDS             1944..3215
                     /gene="hemL"
                     /locus_tag="VGO02_05570"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEV7477192.1"
                     /translation="MKNQSLFERAQRSIPAGVNSPVRAFRAVGGTPPFLERAAGAYLW
                     DAEGRRYIDYVASWGPMVAGHTHPAVVKAVQDAAARALSFGAPTEAEIELAETLRRLV
                     PSLELLRLVSSGTEATMTAIRLARGFTGRSLIVKFEGCYHGHADSLLVKAGSGALTFG
                     NPSSAGVPAETAAHTAVLDFNDAARVRKFFAENGKSVAAVIVEPVAGNMNLVLPAPGF
                     LEALREECTRHGAVLIFDEVMTGFRVARGGAQEKFGIRPDLTTLGKVIGGGLPVGAVG
                     GRRDIMEKIAPLGPVYQAGTLSGNPVAVAAGMATLNLVQAKGFQEKIEATTRSLVDGL
                     IVEAKKANVVFSAQSIGSMFGLYFRAAPPKSFAEVMQCDKDRFNRFFHAMLERGVYLA
                     PSAYEAGFVSAAHTSGDIDATLAAARQSFES"
     gene            complement(3212..4579)
                     /gene="mgtE"
                     /locus_tag="VGO02_05575"
     CDS             complement(3212..4579)
                     /gene="mgtE"
                     /locus_tag="VGO02_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEV7477193.1"
                     /translation="MADVHERLEAEDLQRSLREVQELVQGKRLADLRSSLDRMHPADI
                     AHILEALPPEERLTVWNLVKADRDGDILLEVSDAVRESLIATMDSRELVAAAETLEAD
                     ELADIAGDLPPAVIEEVVRSLSAQEREQLRAALSFPDGSVGALMDFEYTTVREDVTLE
                     AATRYLRRFDELPSHTDQLFVVDREQVLKGTLPLARLIVTDLDRGVGQVLVADSIQFH
                     PEDGAEDAAGAFERYDLVSAPVVDAAGKLIGRLTVDAVVDYIRQRSAESQLAEGGLSQ
                     EEDIFASVVDSFKNRWAWLAVNLVTAFIASRVIGAFEGAIAQLVALAALMPIVAGIGG
                     NSGNQTTTMIVRAHALGQIQGGYWAKLLGKEIGVALLNGVVWGTLLGAIAYAFYGSVA
                     LGGVMALAMILNLVLAAITGVGIPWLRVRFGRDPAAGSSVLLTGVSDSGGFFIFLGLA
                     TLFLV"
     gene            complement(4582..5277)
                     /gene="mtgA"
                     /locus_tag="VGO02_05580"
     CDS             complement(4582..5277)
                     /gene="mtgA"
                     /locus_tag="VGO02_05580"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004567786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEV7477194.1"
                     /translation="MVALWKGFCYSLGALVLAFVLAQLWFFGNIVYLSHYDPGSTAFM
                     EKRLEMLRAKKPGVQLRRIWVPYARISVQLKRAVVAAEDAKFLDHEGFDWEAIQKAMT
                     KNEQRGKVVAGASTISQQVAKNLFLSGERSWLRKGQEAIITWMMERTLSKRRILELYL
                     NVAEWGEGVFGAEAAARYHFGVPASALGTEQAAYLAAVLPSPRRYERAKVTPYIAGRV
                     DTILARMEKVQIP"
BASE COUNT          871 a         1862 c         1780 g          859 t
ORIGIN      
        1 ccgatggcgt cgcgcacgcg cagcgtcgat tgccagttgc ccgccggctc gtgcagcacg
       61 aagccgcggt cggtctgcac cggcccgcca taatgcacgc gcgcctcgcg caattccgaa
      121 tcgcccaatg ggacgttgat ctgctcgaac aacgccgaaa gcgtcatgtc gatcggcttg
      181 ttgatgacaa tgccgagggc gccgttttcg ttgtgctcgc agatgtagat gagcgtgtgg
      241 gcgaagtgcg gatcggccat gctgggcatg gcgatcagga agtggtgcgt cagattgatc
      301 gaggacattc gcccacctgg tattttagcg caatcgggtt agagcgaccc tttttccgaa
      361 aggttgcctc tatatttcga ccatctcgaa gtcgtccttg cgcgcgccgc actccgggca
      421 ggtccagttc atcggcacgt cctcccagcg cgtgccgggg gcgatgccct cgtcgggcag
      481 gccggcggct tcgtcgtaga ggaagccgca gatcaggcac atccacgtct tgaactgcac
      541 ttgagtgtcg gacattgatt tctggcgatg tgaattgcgg gaagtaaaat cggacggata
      601 ttaacccatg gcgaagtcgc ccccgatcgt tctcaccttc gccgcctccg atccgactgg
      661 tggcgccggc ctgcaggccg acgtgctcac gctcgcggcg cgcggctgcc acccgctgtc
      721 ggtcgtcacc gcgctgacgg tgcaggacac gcgcggcgtc gagagcctgc acgcggtcga
      781 agctgcgcgc gtcaccgaac aggcgcactg cctgctcgcg gatttcaagg tggcggcgtt
      841 caagctgggc gttctgggct ccgcgtcgaa cacggcggtg atcgcgcgac tcctcgccgc
      901 ccatcggcac gcgcccgtgg tcgtcgatcc agtgctcgct tcgggacgcg gcgatgcgct
      961 cgccggcgag gacagcatct ccaccatgca gcaagcgatc ttcccgctca ccgcggtggc
     1021 gacgcccaac accctcgagg cgcagcggct cggcggcatc agtgcgatgc tggccgccgg
     1081 atgccgctat gtgctcgtga ccggaacgca cgaggaaggc accgacgtgg tgaaccggct
     1141 gcacgactcg cgcgggctgg ttcgcgaaga ccgctggccg cggcttgcgg gcagctatca
     1201 cggctcgggc tgcacccttg cctcggccct cgccgccgcg ctcgcgaagg gcatggcgat
     1261 gcccgaggcg gtgcgcgacg cgcaggacta cacgtggcac gcgcttgccg cgggctttcg
     1321 ccctggcgcc gggcagttca tcccgcagcg cttcttcagc cagtgaagct gcgcggcctg
     1381 tacgccatca cgccggaagg tgccggtgtc gtcgacaagg cgaggcaggc tctcgaaggc
     1441 ggcatcgcgc tgctgcagta ccggcaaaaa aatcgaacat cccttgagga ggccggggcg
     1501 ctcctcgcgc tcgcccggcg ccacggcgtg ccgttcatcg tcaacgacga tgtcgagctc
     1561 gcgctcgcga ttggcgccga cggcgtgcac ctgggcggcg aggacggcga tctcgccgcc
     1621 gcacgaagca agctcaaggg caagctgctc ggcgcttcgt gctacaaccg gccggagctg
     1681 gcgcaggctg cgctcgccgc gggcgccgac tacatcgcct tcggcagcgt attctcctcg
     1741 ccgaccaagc ccggcgcggt gagggctccg ctttcattgt tcgaaaacaa attgtcaaaa
     1801 cccgtttgtg cgatcggcgg catcacgctg cagaatgcag cgcaggtcat cgccgcaggc
     1861 gccgacatgg tcgccgtaat ctccgacctg ttcgacgcgc cggacatcgc gcgccgggcg
     1921 gccgcgtaca ggaagctatt cccatgaaaa accagtcgct cttcgagcgc gcgcagcgct
     1981 ccatccccgc cggcgtgaat tcgccggtgc gcgcctttcg cgccgtcggc ggcacgccgc
     2041 ccttcctcga gcgtgcggcc ggcgcatacc tgtgggacgc cgagggccgg cgctacatcg
     2101 actatgtcgc ctcctggggc ccgatggtcg ccggtcacac gcacccggca gtggtgaagg
     2161 cagtgcagga cgctgccgcc cgcgcgcttt cgttcggcgc gccgaccgag gcggaaatcg
     2221 agctggcgga gacgctgcgc cgcctggttc cctcgctcga actgctgcgg ctcgtctcgt
     2281 cgggcaccga ggcgacgatg acggcgatcc gccttgcgcg cggcttcacc gggcgcagcc
     2341 tcatcgtcaa gttcgagggc tgctatcacg gccacgccga ctcgctgctg gtgaaagccg
     2401 gctccggcgc cctgaccttc ggcaacccga gctccgccgg cgtgccggcc gagacggcag
     2461 cccataccgc cgtgctcgac ttcaacgacg ctgcgcgcgt caggaaattt ttcgcggaaa
     2521 acggcaagag cgttgcagcc gtcatcgtcg aaccggtcgc cggcaacatg aacctggtcc
     2581 tgcccgcgcc gggattcctc gaggcgctgc gcgaggagtg cacgcgccac ggcgccgtgc
     2641 tgatctttga cgaggtgatg accggctttc gcgtcgcgcg cggcggcgcg caggagaaat
     2701 tcggcatccg gcccgatctc accacgctag gcaaggtgat cggcggcggc ctgccggtcg
     2761 gcgcggtcgg cggccggcgc gacatcatgg agaagatcgc gccgctcggg ccggtgtacc
     2821 aggccggaac gctttcgggc aatcccgtgg cggtggctgc cgggatggcc acattgaatc
     2881 tggttcaggc aaaaggcttt caggagaaga tcgaggcgac cacgcgctcg ctcgtcgacg
     2941 gcctgatcgt cgaagcgaag aaggccaacg tcgtcttctc ggcgcaatcc atcggcagca
     3001 tgttcggcct gtatttccgc gccgcgccgc cgaagagctt cgccgaggtc atgcagtgcg
     3061 acaaggaccg cttcaaccgc ttcttccatg ccatgctcga gcgcggcgtg tacctcgctc
     3121 cctcggccta tgaggcgggc ttcgtctcgg ccgcgcacac ctcgggcgac atcgacgcca
     3181 cgctcgccgc cgccaggcaa tccttcgaga gctagactag gaacagcgtc gccagaccga
     3241 ggaagatgaa gaagccgccc gagtcggaca ccccggtgag cagcaccgag gagccggccg
     3301 ccgggtcgcg gccgaagcgc acgcgcagcc acgggatgcc gaccccggta attgcggcca
     3361 gcaccagatt gagaatcatg gcgagcgcca tgactccgcc gagcgcgacg ctgccgtaga
     3421 acgcgtaggc gatggcgccg agcagcgtgc cccacaccac gccgttcagc agcgcgacgc
     3481 cgatctcctt gccgaggagc ttcgcccagt agccgccctg gatctggccg agcgcgtgcg
     3541 cgcgcacgat catggtcgtg gtctggttgc cggaattgcc gccgatgccg gcgacgatcg
     3601 gcatcagcgc ggcgagcgcc acgagctgcg cgatcgcgcc ttcgaaggca ccgatgacgc
     3661 gcgaggcgat gaacgcggtg accaggttca ccgccagcca ggcccagcgg ttcttgaagc
     3721 tgtcgacgac cgaggcgaag atgtcctctt cctgcgacag gccgccttcg gcgagctgcg
     3781 attcggcgct gcgctggcgg atgtagtcga ctaccgcgtc gaccgtgagc cggccgatca
     3841 gcttgccagc ggcgtcaact accggtgccg aaaccaggtc gtagcgctcg aatgcgccgg
     3901 ccgcgtcctc ggccccgtcc tccggatgga attgaatgct gtcggccacc agcacctggc
     3961 cgactccgcg gtcgaggtcc gtcacgatga ggcgcgcaag cggcagcgtc cccttcagca
     4021 cctgttcgcg gtcgacgaca aacagctgat cggtatggct cggcagctcg tcgaaacggc
     4081 gcagatagcg cgtcgccgcc tcgagggtca cgtcctcgcg caccgtggtg tactcgaaat
     4141 ccatcaacgc cccgaccgag ccatccggga aggaaagcgc cgcgcgcagc tgctcgcgct
     4201 cctgcgccga gagggagcgc accacctctt cgatgaccgc cggcggcagg tcgccggcga
     4261 tgtcggccag ctcgtcggcc tccaaggtct cggccgcggc gaccagctcg cgcgagtcca
     4321 tggtcgcgat gagcgactcg cgtaccgcgt cggaaacctc gagcaggatg tcgccgtcgc
     4381 ggtcggcctt gaccaggttc cacaccgtga ggcgctcctc cggcggcagc gcctccagga
     4441 tgtgcgcgat gtcggccgga tgcatgcggt ccaggctcga gcgcagatcc gccaggcgct
     4501 tgccttgcac cagctcctgc acctcgcgca ggctgcgctg caggtcctcg gcttcgagcc
     4561 gctcgtgcac gtcggccatg gtcaggggat ctgcaccttt tccatgcgcg cgaggatcgt
     4621 gtcgacccgc ccggcgatat agggggtcac cttggcacgc tcgtagcgcc gcgggctggg
     4681 caggaccgcg gcgaggtacg ccgcctgctc cgtgccgagc gccgaggccg gcacgccgaa
     4741 gtggtagcgc gccgccgcct ccgcgccgaa cacgccctcg ccccactcgg cgacgttgag
     4801 gtacagctcg aggatccggc gcttggagag cgtgcgctcc atcatccagg tgatgatggc
     4861 ttcctgcccc ttgcgcagcc aggagcgctc gccggaaagg aacaggttct tcgccacctg
     4921 ctggctgatg gtcgaggccc cggcgactac tttcccgcgc tgctcattct tggtcatcgc
     4981 cttctggatc gcctcccagt cgaatccctc atggtcgaga aacttggcgt cctcggcggc
     5041 gaccaccgcg cgcttgagct gcaccgaaat ccgcgcgtag ggcacccaga tgcgacgcag
     5101 ctgaacgccg ggcttcttcg cacgcagcat ctcgagacgc ttctccatga aggcggtcga
     5161 ccccgggtca tagtgcgaca ggtagacgat attgccgaaa aaccagagct gcgcgagcac
     5221 gaaggcgagg accagcgcgc ccaggctgta gcaaaagccc ttccacagcg cgaccagcac
     5281 gcgcagcgcg ccgccgcgcc tagccgccac gcagcttccg cagcaccagc gcggtttcgg
     5341 ggcgcacgcc gcgccagacg aagaacgatt cc
//
LOCUS       DASYJA010000261         7311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2672624, whole genome shotgun sequence.
ACCESSION   DASYJA010000261 DASYJA010000000
VERSION     DASYJA010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7311
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2672624"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VGO02_05585"
     CDS             <1..384
                     /locus_tag="VGO02_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV7477195.1"
                     /translation="GRRDALWAAEQVLRAGTCRALLAWFHRASYPELQRLAAAAESGR
                     ACVVLFRPREAEREPSPACLRIALEPAAGELAVRILKRRGPPAAGPLRVPLNRPAHAL
                     GRPAFSVPACRQPRAHRGMGLPVHP"
     gene            299..1462
                     /locus_tag="VGO02_05590"
     CDS             299..1462
                     /locus_tag="VGO02_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEV7477196.1"
                     /translation="MLWVALHFQSLPADSLERIAAWACQFTPKVSLEPPRELLMEVQG
                     SLRRFGGSHGLLEKLRAGLAATGFEAVLATAPAPRAALWLARGGCRVLEEVPVEAVCA
                     ADALEFLRSIGVQTLGELLALPREGLARRCGPALVDDLDRALGALPEGRVFFAPPARF
                     AARLELPAPVSHAEGLLFAARRLLVQLEGLLAARQAGIRGFKLLLGNQCVEIGLASPA
                     RDAERLSRLLRERLATLVLTRPVESIGLEAGDFVPLAGRTAGMFGDALAEAEDWAQLL
                     ERLQVRLGRGAVHGLATYPDHRPELAWRAVEPGEWEPREFRHPGPRPLWLLEPPRRLG
                     QQAPLLLAGPERVEAGWWDGDEARRDYFIAQDEASMTWVYREDGEWYLHGFFA"
     gene            1446..4460
                     /locus_tag="VGO02_05595"
     CDS             1446..4460
                     /locus_tag="VGO02_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEV7477197.1"
                     /translation="MDFLPDYAELHCLSNFSFLRGASHPQELVERAAALGYKALALTD
                     ECSLAGVVRAHTAAKELGLKLIVGTEVTVEQEKLVLLATDRRSYGAISSLITAGRRRG
                     KKGSYSLSRPDVERLSGSGAAVLWVPQEDFGFAEWVKEKFASAWIAAELHCGSGDRAR
                     LGRLRDISRRYGLPLVAAGDVHMHVRSRRKLQDVATAVRLGRPLAQCGHALYPNAERH
                     LRLRVRLAQLYPAELLAETVALAERCHFSMDQLRYEYPEELVPPGETPATWLRHLVEE
                     GLKKRFPVCLPEKVRQLAERELALIAELRYEPFFLTVHDVVRYARSKDILCQGRGSSA
                     NSIVCYALEITAVGPERLEMLFERFISKERDEPPDIDVDFEHERREEVIQYIYGKYTR
                     SRAAIAATVICYRPKSALRDVGKALGFEAADLERLAGNLAWWDGRRAMPERVAESGFD
                     PSSLAAQQLCALAGELVGFPRHLSQHVGGFVISRGPLSELVPIENAAMPERTVIQWDK
                     DDLEALGLLKVDVLALGMLSALKRAMNFLEIKELTEIPENDAAVYAMMQKADTVGVFQ
                     IESRAQMSMLPRLKPKDFYDLVVQIAIVRPGPIQGDMVHPYLRRRAGLEKVEYPSDEV
                     KQVLERTLGVPIFQEQVMQLAIKAAGFSPGEADQLRRAMAAWKRKGGLEKFERKLKDG
                     MRQKHYSDQFADAVYRQICGFGEYGFPESHSASFALLAYASSWIKHHHPALFCAALLN
                     SQPMGFYQPAQLVQDARRHGVEVRPPDVNLSDWDCTLEDGALRLGLRRVGGLPEATGR
                     SIARGRPHLSIAALQLNRKELRCLAAAGALQSLAGHRRLAHWAAAGAQRSAPLDVASP
                     ERRPQLAPPSEGQDIVADYASLGLTLGRHPLALLRGHLKKLQTIEARALQLLHSGSQA
                     RVAGLVTCRQRPDTASGVIFVTLEDETGCMNVVVWRRLIERQRKELLGARLLGVQGVI
                     ERDGEVVHLIARRLFDHSALLGPLATSSRDFH"
     gene            4519..4824
                     /locus_tag="VGO02_05600"
     CDS             4519..4824
                     /locus_tag="VGO02_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEV7477198.1"
                     /translation="MNENSHKVTIAKDLEDLIPVFLKNRHKEVDTLRVALASADFEQL
                     RQLGHRMKGVGNSYGFERVSVIGKQIEDGARSGDRSALEASIAGYAEYLARVEVAYE"
     gene            4817..5941
                     /locus_tag="VGO02_05605"
     CDS             4817..5941
                     /locus_tag="VGO02_05605"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7477199.1"
                     /translation="MSDLAAAERLAATQSTFRVLIVDDDPDMVAFLGRLLSREGMKVD
                     AEYDGQAALAHIAASPPDLVLLDVLMPGVSGFEVCRRLKADPATALIPVILVTALEDH
                     ASRVRGIDIGADDFLSKPVNREELVARVKNLRRLHQTRRELEGRRLATEIGHKEAIRK
                     TFSRYISPRLADRIIAESGEPPFDNEAQRINVVALFADLRGFTRLTETLEVGEVVGML
                     NEYFAVITDAAYRHEGTIFSMAGDCLLVGFNVPFPQPDAALRAWHTAQEMIAGFSPVL
                     ASWTKRGGMSTGVGIGIAAGEAIIGNIGSPHHMSYTIIGDAVNTAARLMQMAKASEML
                     VSGPAYEAIRGAVPAGSVEARGEVALRGKSGATPVYSIRL"
     gene            5958..7184
                     /locus_tag="VGO02_05610"
     CDS             5958..7184
                     /locus_tag="VGO02_05610"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV7477200.1"
                     /translation="MRILIIDDDDDLRTLLAHHIREQWPQAEIEQFDPLENELPAADY
                     PLGTFDVMILDYMLGRGDGLQWLQQFKKRADCPKVLFLTGAGNEIIAVRAMKAGADDY
                     QRKQELTRERLVTSIRDLTANSVERTLTPELAVRMEGQSLGARLKVPGIRVLHMIGEG
                     GASRVYLASRELDDEPLVVKILRPEITADQVGLARFMEEYSLVERIQSPHVARILGHG
                     ISEGHAYLVMEFFSGGDLNKRLDGRALAPDEALKLFRELMFALGDIHEKGVLHRDLKP
                     QNLMFRQDGSLAIVDFGIAKHIDGIDRTGHGEILGTPRYMSPEQVQGRALDLRTDIYS
                     AGVLLFQMLTGYHLFEGDTAVEVALHHLNTEPPPMPEELARYQRLMDKLIEKDRDARF
                     RNADEVIGFLSRTFYQ"
BASE COUNT         1144 a         2472 c         2547 g         1148 t
ORIGIN      
        1 ggcaggcgcg atgcgctctg ggcggccgag caggtgctgc gcgcgggaac ctgccgcgcg
       61 ctgctggcat ggttccatcg cgcgagctac cccgagctgc agcggctcgc cgcggccgcc
      121 gaatccgggc gcgcctgcgt cgtgctgttc cggccgcgcg aagccgagcg cgagccttcg
      181 cctgcctgcc tgcgcattgc gctggagccc gccgccggcg agcttgcggt gcgcatcctc
      241 aagcgccgcg gcccccctgc agccgggccg ctgcgcgtgc ctctcaacag gcccgctcat
      301 gctctgggtc gccctgcatt ttcagtccct gcctgccgac agcctcgagc gcatcgcggc
      361 atgggcctgc cagttcaccc ctaaggtttc gctcgagccg ccgcgcgagc tgctgatgga
      421 ggtgcaaggc agcctgcgcc gcttcggcgg cagccacggc ttgctcgaaa aattgcgcgc
      481 cgggctcgcc gcgaccggct tcgaggcggt gctcgccact gcgcctgcgc cgcgcgccgc
      541 gctttggctg gcgcgcggcg gctgccgggt gctcgaagaa gtgccggtcg aggctgtgtg
      601 cgccgcagat gcgctcgaat tcctgcgcag catcggcgtg cagacgctcg gcgagctgct
      661 cgcgctcccc cgcgaaggcc tggcgcggcg ctgcggcccg gcgctggtcg acgatctcga
      721 ccgcgccctg ggtgccttgc ccgaagggcg ggttttcttc gccccgccgg cgcgctttgc
      781 cgccaggctc gagctgccgg ctccggtaag ccatgccgag ggcctgctct tcgccgcgcg
      841 ccggctgctg gtccagctcg aggggttgct tgccgcgcgc caggccggga tacgcggttt
      901 caagctcttg cttggaaatc aatgcgtcga aatcggcctt gcttctccgg cccgggatgc
      961 cgagcgtctt tcccgtctgc tgcgcgagcg gctggcgacg ctcgtgctga cgcggccggt
     1021 cgagagcatc ggcctggagg ccggggattt cgttccgctc gccggacgca cggcgggaat
     1081 gttcggcgac gcgctcgccg aggccgagga ctgggcgcag ctgctggagc ggctgcaggt
     1141 gcgcctgggc cgcggcgcgg tgcacggcct ggcgacctac ccggaccacc gccccgagct
     1201 cgcctggcgc gcggtcgagc cgggcgagtg ggagccgcgc gagttcaggc accccggccc
     1261 gcggccgctc tggctgctcg agccgccgcg ccggctcggc cagcaggcgc cgctgcttct
     1321 cgccggtccg gagcgcgtcg aagccggatg gtgggacggc gacgaggcgc ggcgcgacta
     1381 tttcatcgcc caggacgagg cttccatgac ttgggtgtat cgcgaagacg gagagtggta
     1441 cctgcatgga ttttttgcct gattacgcgg agttgcactg cctgtcgaac tttagctttc
     1501 tgcgcggggc ttcgcatccg caggagctgg tggagcgcgc cgcggcgctc ggctacaagg
     1561 cgctcgcgct caccgacgag tgctcgctcg ccggggtagt gcgcgcgcat accgctgcga
     1621 aggagctcgg gctgaagctg atcgtcggca ccgaggtcac ggtggagcaa gagaagctgg
     1681 tgctgctcgc caccgatcgg cgctcctacg gcgccatttc gtcgctcatc accgccggcc
     1741 ggcgccgcgg caagaaaggc agctattcgc tgtcccgccc ggacgtggag cggctttccg
     1801 gtagcggcgc ggccgtcctg tgggtgccgc aggaggactt cggcttcgct gaatgggtga
     1861 aggaaaaatt cgcgagcgcc tggattgcgg ctgagctgca ttgcggatcc ggcgatcgcg
     1921 ccaggctcgg gcgcttgagg gacatttcca ggcggtacgg gctgccgctg gtggcagcag
     1981 gcgatgtaca catgcatgtg cgctcccgca ggaagctgca ggacgtggcc acggcggtgc
     2041 ggctcggccg gccgctggcg caatgcggcc acgcgctgta tccgaacgcc gagcgccatc
     2101 tgcggctgcg cgtgcgcctg gcgcagctct atccggcgga gctgctggcg gaaacggtgg
     2161 cgctcgccga gcgctgccat ttctccatgg accagctgcg ctacgagtat cccgaggagc
     2221 tggtgccgcc cggcgagacg ccggcgacct ggctgcgcca tctcgtcgaa gaggggctga
     2281 agaagcgctt ccccgtttgt ttgccggaaa aggtgaggca gcttgccgag cgcgaattgg
     2341 cgctgatcgc tgaactgcgc tacgagccat tcttcctgac ggtccacgat gtggtccgct
     2401 acgcgcgctc gaaagacatt ctttgccagg gcaggggttc gtcggccaat tcgatcgtct
     2461 gctacgcgct ggagatcacc gcggtcggcc cggagcggct ggaaatgctg ttcgagcgct
     2521 tcatctcgaa ggagcgcgac gagccgccgg acatcgacgt cgacttcgag cacgagcggc
     2581 gcgaggaagt catccagtac atctacggca agtacacgcg cagccgcgcc gccatcgcgg
     2641 cgacggtgat ctgctaccgg ccgaagagcg cgctgcgcga cgtcggcaag gcgctggggt
     2701 tcgaggcggc ggacctcgag cgcctcgccg ggaacctggc ctggtgggat ggccggcggg
     2761 cgatgcccga gcgcgtcgcc gagtcgggct tcgatccgtc cagcctcgcc gcgcagcagc
     2821 tgtgcgcgct ggcgggcgag ctggtcggct ttccgcgcca cctttcgcag cacgtcggcg
     2881 gcttcgtcat ctcgcgcggg ccgctctccg agctggtgcc gatcgagaac gctgccatgc
     2941 cggagcgcac ggtgatccag tgggacaagg acgatctcga ggcgctgggc ctcctgaagg
     3001 tcgacgtgct ggcgctcggc atgctcagcg cgctcaagcg cgcaatgaat ttcctggaaa
     3061 taaaggaatt gacggagatt ccggaaaacg atgcggccgt ctacgcgatg atgcagaagg
     3121 ccgacaccgt gggcgtgttc cagatcgagt cgcgggcgca gatgagcatg ctgccgcgcc
     3181 tcaagccgaa ggatttctac gacctggtgg tgcagatcgc gatcgtgcgg cccggcccga
     3241 tccagggcga catggtgcac ccctatcttc gccgccgggc cggcctcgag aaggtcgaat
     3301 acccgagcga cgaagtgaaa caggtgctcg agcgtacgct cggcgtcccc atcttccagg
     3361 agcaggtgat gcagctcgcc atcaaggccg ccggcttctc gcccggggag gccgaccagc
     3421 tgcgccgcgc catggcggca tggaagcgca agggcgggct ggagaaattc gagcgcaagc
     3481 tcaaggatgg catgcggcag aaacattatt cggaccaatt cgccgatgcc gtctaccgcc
     3541 agatctgcgg cttcggcgaa tacggctttc cggaatcgca ctcggcgagc ttcgcgctgc
     3601 tggcgtatgc ctcgtcctgg atcaagcatc accacccggc gctattctgc gcggcgctgc
     3661 tcaacagcca gccgatgggc ttctaccagc ccgcgcaact ggtgcaggac gcgcggcgcc
     3721 atggcgtgga agtgcggccg ccggacgtga acctgagcga ctgggactgc acgctggaag
     3781 acggggcgtt gcggctcggg ctgcgcaggg tgggcggact gccggaagcc acgggaagaa
     3841 gcatcgctcg gggcaggccg catctttcca tagccgcgct gcagctcaac cgcaaagagc
     3901 tgcgctgcct cgccgcggcg ggcgcgctgc agtcgctggc cggccatcgg cgcctggcgc
     3961 actgggcggc ggcaggagcg cagcgcagcg cgcccctcga cgtcgcctcg cccgagcggc
     4021 ggccgcagct tgcgccaccg agcgaagggc aggacatcgt cgccgactac gccagcctcg
     4081 gcctcaccct tggccggcac ccgcttgccc tgctgcgcgg acatttaaag aaactgcaaa
     4141 ctatcgaggc ccgggcgctg cagctgctgc acagcggcag ccaggcgcgg gtcgccgggc
     4201 tggtcacctg ccggcagcgg ccggatactg cgagcggtgt gatcttcgtc acgctcgagg
     4261 acgagaccgg gtgcatgaac gtggtcgtct ggcggcggct gatcgagcgc cagcgcaagg
     4321 agctgctcgg cgcgcgcctg ctcggcgtgc agggagtgat cgagcgggac ggcgaggtgg
     4381 tgcatctcat cgcccggcgc ctgttcgatc acagcgcgct gctcggcccg ctcgccacga
     4441 gctccaggga tttccactga gggggctcac aaaccataac agtgggctgt cgccgccgcc
     4501 cgcctataat tgaagaacat gaacgaaaac agccacaaag tcaccatcgc gaaagacctg
     4561 gaagacctga ttcccgtgtt tctcaagaac cgccacaagg aagtcgatac cttgcgggtg
     4621 gcgcttgcct cggcggattt cgagcagctg cgccagctcg ggcaccgcat gaaaggggtc
     4681 ggcaattcct acggcttcga gcgggtgtcc gtcatcggca agcagatcga ggacggcgcg
     4741 cgcagcggcg accgctccgc cctcgaggcg agcatcgcgg gttacgccga gtacctggca
     4801 cgggtcgaag tggcttatga gtgatctggc tgccgccgag cggctcgcag ccacacagag
     4861 cacgttccgc gtcctgattg tcgacgacga tccggacatg gtggcatttc tcggccgcct
     4921 gctttcgcgc gagggcatga aggtcgacgc cgagtacgac ggccaggcgg cgctcgcgca
     4981 catcgcggcc tccccgccgg acctggtgct gctcgacgtg ctgatgccgg gggtgagcgg
     5041 cttcgaggtg tgccggcggc tcaaggccga tcccgccacg gcgctgatcc cggtcatcct
     5101 ggtaacggcg ctcgaggacc atgccagccg ggtgcgcggc atcgacatcg gcgccgacga
     5161 cttcctgtcc aagccggtga accgcgagga gctggtcgcc cgggtcaaga acctgcgccg
     5221 gctgcaccag acgcggcgcg agctcgaggg gcgccgcctg gccaccgaga tcgggcacaa
     5281 ggaggcgatc cgcaagacgt tctcgcgcta catctcgcca cggctcgccg accgcatcat
     5341 cgccgagtcc ggcgagccgc ccttcgacaa cgaagcgcaa cgcatcaacg tggtggccct
     5401 gttcgccgac ctgcgcggtt tcacgcgtct caccgagacg ctcgaggtgg gcgaggtggt
     5461 cggcatgctg aacgaatact tcgcggtcat caccgacgcc gcctaccgcc acgagggcac
     5521 catcttcagc atggccgggg actgcctgct ggtcggcttc aacgtgccgt tcccgcagcc
     5581 cgacgcggcg ctgcgcgcct ggcacaccgc gcaggagatg atcgccggct tctcgccggt
     5641 gctcgccagc tggaccaagc gcggcggcat gtcgaccggc gtcggtatcg gcatcgcggc
     5701 cggcgaagcc atcatcggca acatcggctc gccccaccac atgagctaca cgatcatcgg
     5761 cgacgcggtg aacaccgcgg cgcggctgat gcagatggcg aaggcgagcg agatgctggt
     5821 ctcgggcccg gcctacgagg cgatccgcgg cgcggtgccg gccggaagcg tcgaggcgcg
     5881 cggcgaagtg gcgctgcgag gcaagagcgg ggccacgccg gtgtattcga tcaggctata
     5941 gcttgtaacc tgcattcgtg cgcatcctca tcatcgacga cgacgacgac ctgcgcacgc
     6001 tcctcgcgca ccacatccgg gagcagtggc cgcaggcgga gatcgagcag ttcgatcccc
     6061 tggagaacga gctgccggcc gcggactatc cgctgggcac gttcgacgtc atgatcctcg
     6121 actacatgct aggacgcggc gacgggctgc aatggctgca gcagttcaag aagcgcgccg
     6181 actgcccgaa ggtgcttttt ttgaccggcg ccggcaacga gatcatcgcg gtgcgcgcca
     6241 tgaaggccgg cgccgacgac taccagcgca agcaggagct gacgcgcgag cgcctggtca
     6301 cctcgatccg cgacctgacc gccaactcgg tcgagcgcac gctgacgccc gagctcgcgg
     6361 tgcgcatgga gggccagtcg ctcggcgcgc gcctcaaggt ccccggcatc cgcgtgctcc
     6421 acatgatcgg cgagggcggc gcctcgcgcg tctacctggc ctcgcgcgag ctcgacgacg
     6481 agcccctggt ggtgaagatc ctgcgtccgg agatcaccgc cgaccaggtc ggcctggcgc
     6541 gcttcatgga ggagtactcg ctggtggagc gcatccagag cccccacgtg gcgcgcatcc
     6601 tcggccacgg catctccgaa gggcacgcct acctggtgat ggagttcttc agcggcggcg
     6661 acctcaacaa gcggctcgac ggccgcgcgc tcgcgcccga cgaggcgctc aagctgttcc
     6721 gcgagctgat gttcgcgctg ggcgacatcc acgagaaagg cgtcctgcac cgcgacctga
     6781 agccgcagaa cctcatgttc cggcaggacg gcagcctggc gatcgtcgat ttcggcatcg
     6841 ccaagcacat cgacggcatc gaccgcaccg gccatggcga gatcctgggc acgccgcgct
     6901 acatgagccc ggagcaggtg cagggccgcg cgctcgacct gcgcaccgac atctacagcg
     6961 ccggcgtgct gctcttccag atgctcaccg gctaccacct gttcgaaggg gatacggcgg
     7021 tggaagtcgc cctgcaccac ctcaataccg agccgccgcc gatgcccgag gagctcgccc
     7081 gctaccagag gctgatggac aagctgatcg agaaggaccg cgacgcacgc ttccgcaacg
     7141 ccgacgaagt gatcggcttc ctctcgcgca ccttctacca gtaattcgtc agggcagttt
     7201 cgcccagatt gccctgagct gttccggcag atccttcggc gagaaaggct tggagatcgc
     7261 gccggctgcg cccagggcgc tgagctcgtc gagctcggtc tgcgaagcct t
//
LOCUS       DASYJA010000262         5259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2944819, whole genome shotgun sequence.
ACCESSION   DASYJA010000262 DASYJA010000000
VERSION     DASYJA010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2944819"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..2061)
                     /locus_tag="VGO02_05615"
     CDS             complement(139..2061)
                     /locus_tag="VGO02_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV7477201.1"
                     /translation="MVLLALAGSAFAQPAQRDLADLSLEELANVEVTSVSRRPERLAD
                     APASIFLITAEDIRRSGARSLPEALRLAPNLYVAQVSASGYAISARGFTSTAANKLLV
                     LIDGRSVYTPLFSGVFWDVQDVLLEDIDRIEVISGPGGPLWGVNAVNGVINVITRSAA
                     DTQGVMATAGTGNGDSEGALRYGGKLGADGHYRVYGKAFERYATVQENGSTKNDAWHN
                     GQAGFRADWSRSGNRVSVNGNVYQGEEGQPLPGSISVTGVAVPLGDTSISGANLTARW
                     DRALEGGSSLTVQGYFDRTERTVRPQFAETLDIFDLQLQHSLRPIGSHSLVWGAGYRH
                     SQDQVTNSTFLAFTPPRPFIGFLPERVSQDWPSLFAQDEVTLRDDLRLTIGARLERND
                     YTGNEFLPSARLAWKVRPEHLLWTAASRAVRAPSRLDRDTFVPAAPPFLLAGGPDVRS
                     EVAKVYEVGYRGQPARPLSISVTAFRTDYEDLRSQEIAPSGTFLFFANGMEGKSTGIE
                     AWGTLRASATWRLSAGATFLDQDLHPKPGSNDTNVAALGNDPTHQFRLRSVHDLPYRQ
                     ELDIIARYVGALPNPSVPRYTAVDVRYAWRLHPELELSLTVQNLLDDRHAEFSPAATR
                     TDLERGWFIRLKWTSR"
     gene            2261..3976
                     /locus_tag="VGO02_05620"
     CDS             2261..3976
                     /locus_tag="VGO02_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HEV7477202.1"
                     /translation="METKLKLAPRETEVEKRADGSMLLRSPQRLGGYARCVTEWLVQW
                     SDRAPDRVFLAERASSGWKTLNYREAYGAVRRVGQYLLDKGLDKDKPVAILSDNGIDH
                     ALLSLGAMHVGVPAAPISPAYSLMSKDFGKLKMIFELLQPGLVYAADPQKFGPALEAV
                     GAKSASVAEILETNPGSTLEREFSKISGKTIAKILFTSGSTGAPKGVINTHEMLCANQ
                     QMMAQAWPFLEDKPPVVVDWLPWNHTFGGNHNFNMVLRNGGTLYIDGGKPAPGLIETT
                     ARNLAEVAPTMYFNVPRGFDLLLPFLEKDAALRQNFFRDLDMLFYAAAALPQNLWDRL
                     IELARKEKKAPAMLSAWGSTETSPLATSVHFHMERPGVIGLPVAGCELKLVPAAGKLE
                     VRVRGPHVTPGYYKRPDLTAAAFDEEGFYRIGDAVKLADPNDATRGVVFDGRVAEDFK
                     LSTGTWVHVGALRVKLIAAGDPLIQDAVITGHDRDAVGALLFLSPAAKGMDVKEKLIQ
                     ILKSFEEEPSSTRITRLMIMSEPPSIDANEITDKGYMNQRAVLERRAALVDRLYSGID
                     VVTTS"
     gene            complement(3966..4832)
                     /locus_tag="VGO02_05625"
     CDS             complement(3966..4832)
                     /locus_tag="VGO02_05625"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV7477203.1"
                     /translation="MEPKLPPRLGIVILMVIAVTFGANHISARIAFDHGASVVTGVFA
                     RSLFTSLAVFALLRLQGVPIALSRAQLGRAALIGVALAVQSYCLYSAVSQIPVALALL
                     AFNTFPVMLSLMTWAAGRGRPSARAAIAMPIALVGLSIALDVWGKPVGPGVLWALAAG
                     FAFATALFLTDLWLKAVDGRIRSALTMATVALIVAVIGAALGAFRAPADATGWMGVAL
                     LSVFYGLAITSVFVVLPRMGSASYAVVLNFEPISLLLLGWVILGQAIAPLQIVGAFIV
                     VGAIAWLGLARK"
     gene            4831..>5259
                     /locus_tag="VGO02_05630"
     CDS             4831..>5259
                     /locus_tag="VGO02_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV7477204.1"
                     /translation="MSPLKNVRVIEFCQIAAGPFAGLLLADMGADVIKVESPEGDGMR
                     NWPPHTAGYSENFASLNRGKKSVVLNLKTPSDVENAIRLCLSADVVLENFRPGVMNRL
                     GLGYEVLKNQKPEIVYCSISAFGQEGPRSQEGGFDVTLQAI"
BASE COUNT          977 a         1769 c         1695 g          818 t
ORIGIN      
        1 cgctcggcgg ccattcgacg aagctgccga acttgtagag gtacgcggcc ttgatctcca
       61 gctcgcggtc cgtctgcgcg aagaccggcg cggcggcgaa cagcgcgtat aggacgacaa
      121 cgtgcgcgaa caggcgggtc accgcgacgt ccatttcagc ctgatgaacc aaccccgctc
      181 gaggtcggtg cgcgtcgccg caggactgaa ctcggcgtgg cgatcgtcga gcaaattctg
      241 cacggtgagc gagagctcga gctcggggtg aagccgccag gcgtagcgca cgtcgacggc
      301 cgtgtagcgc ggcacgcttg gattgggcaa ggcaccgaca tagcgcgcga tgatgtccag
      361 ctcttgcctg tagggaaggt cgtgcaccga acgcagccgg aactggtggg tcgggtcgtt
      421 gcccagcgcc gcgacgttgg tgtcgttgct cccaggcttg ggatgcagat cctggtcgag
      481 gaacgtggcc ccggcgctca gacgccaggt cgcgcttgcc ctgagcgtgc cccaggcctc
      541 gattccggtc gacttgccct ccatgccgtt ggcaaagaag aggaacgtgc cgctcggtgc
      601 gatctcctgg ctgcgcaggt cctcgtagtc ggtacggaaa gcggtgacgg aaatggacag
      661 cgggcgcgca ggctgcccac ggtatccaac ctcgtatacc ttggccactt cggagcgcac
      721 gtccggcccg ccggcgagca ggaacggcgg cgccgccgga acgaacgtgt cgcggtcgag
      781 ccgggacggg gcgcgtacgg cgcgcgacgc cgccgtccac agcaggtgct ccgggcggac
      841 cttccacgcc agccgcgcgc tgggcaggaa ttcattgccg gtatagtcgt tgcgctccag
      901 tctggccccg atcgtcaggc gcaggtcgtc gcgcagagtc acctcatcct gcgcgaacag
      961 gctcggccag tcctggctga cccgttccgg caggaaaccg atgaagggcc gcggcggcgt
     1021 gaacgcaagg aaagtgctgt tcgtgacctg atcctggcta tggcggtacc cggcgcccca
     1081 caccagcgaa tggctgccga tcggccgaag cgagtgctgc aactgaagat cgaagatgtc
     1141 cagcgtctcg gcgaactgcg ggcggacggt acgctcggtc cggtcgaaat agccctgcac
     1201 tgtcaggctg gaaccacctt ccagcgcgcg gtcccagcgg gcagtcaggt tggcgcccga
     1261 tatggaagtg tcaccgagcg gaaccgccac cccggtcacc gaaatactgc cgggaagcgg
     1321 ctgaccttcc tccccctggt acacgttgcc gttgacgctg acgcggttcc cgctgcggct
     1381 ccagtcggcc cgaaatcccg cctgcccgtt gtgccaggca tcgtttttcg tgctgccgtt
     1441 ctcctgcacg gtggcatagc gctcgaaggc cttcccgtag acccggtaat gcccgtcggc
     1501 gccgagcttc ccgccgtagc gcagggcacc ctcgctgtcg ccgtttccgg ttccggcagt
     1561 cgccattacg ccttgggtgt ctgcggccga gcgcgtaata acgttgatga cgccgttgac
     1621 ggcattcacg ccccagagcg ggccaccagg cccgctgatc acctcgatgc gatcgatgtc
     1681 ctcgagaagc acatcctgga cgtcccagaa cacgccggag aacaacggcg tatagaccga
     1741 acgcccgtcg atcagtacca gaagcttgtt cgcggcggtg ctggtgaaac cacgggcgct
     1801 gattgcgtac ccgctggcgc tcacctgcgc gacgtacagg ttcggggcca gccgcagcgc
     1861 ttcgggcagg ctcctcgcgc ccgagcggcg aatgtcttcg gcggtgatga ggaatatcga
     1921 agcgggcgcg tcggcgagac gctcagggcg ccgcgacacc gacgtcacct cgacgttggc
     1981 gagctcctcc aggctgaggt cggccaaatc gcgctgcgcg ggctgtgcga acgcactgcc
     2041 cgcgagtgcg agtagcacca tccctccctt cccgcgcatc cccacccctt tgcccgtttg
     2101 ttctggtatc ggacactcgc agactatcag ggcggatcgc gaggttttgt agggaaatcc
     2161 ctacaatgca gtgcacagct agtgcgtttt ctcaccaaat tgcgggtcca gcggcccccc
     2221 ggacaaatct tcatacaatc cgcaccgcag ggccccgaca atggagacaa agctgaaact
     2281 cgccccgcgc gaaactgagg tcgaaaaacg cgccgacggc agcatgctgc tgcgctcgcc
     2341 gcagaggctc ggcggctacg cccgctgcgt caccgaatgg ctcgtgcagt ggtcggaccg
     2401 cgcgcccgac cgggttttcc tcgctgaaag agcttcttca ggatggaaaa ccctcaacta
     2461 tcgcgaagcc tacggcgccg tgcgccgcgt cggccagtac ctgctcgaca aggggctcga
     2521 caaagacaag ccggtcgcga ttctttccga caacggcatc gaccacgccc tgctgagcct
     2581 cggcgcgatg cacgtcggcg tgccggcggc gccgatctct cccgcctact ccctcatgtc
     2641 gaaggacttc ggcaagctga agatgatctt cgagttgctg cagccgggcc tcgtctacgc
     2701 cgcggacccg cagaagttcg gcccggcgct cgaggccgtc ggagcgaagt cggcttcggt
     2761 agcggagatt ctcgagacca atcctggctc gaccctcgaa agggaatttt ccaaaataag
     2821 cggaaaaaca attgccaaaa tactcttcac ctccggctcc accggcgcgc cgaagggtgt
     2881 gatcaacacg cacgagatgc tgtgcgcgaa ccagcagatg atggcgcagg cctggccgtt
     2941 cctcgaagac aagccgccgg tggtggtcga ctggctgccg tggaaccaca ccttcggcgg
     3001 caaccacaac ttcaacatgg tgctgcgcaa cggcggcacg ctgtacatag acggcggcaa
     3061 gccggcgccg ggcctgatcg agaccaccgc gcgcaatctc gccgaggtcg cgccgaccat
     3121 gtatttcaat gtgccgcgcg gcttcgacct gctgctgccc ttcctggaga aggacgcggc
     3181 gctgcggcag aacttcttcc gcgacctcga catgctgttc tacgccgctg cggcgttgcc
     3241 gcagaacctc tgggatcgcc tgatcgaatt ggccaggaag gagaaaaagg cgccggccat
     3301 gctgtccgcc tggggctcga ccgagacctc gccgctcgcc acctcggtgc acttccacat
     3361 ggagcgccct ggagtcatcg gcctgccggt ggcgggatgc gagctgaagc tggtgccggc
     3421 ggccggcaag ctcgaggtgc gcgtgcgcgg cccgcacgtc acgcccggct actacaagcg
     3481 cccggacctg accgcggctg ccttcgacga ggaaggcttc taccgcatcg gcgacgcggt
     3541 gaagctcgcc gacccgaatg acgcaacccg cggcgtggtc ttcgacggcc gcgtcgccga
     3601 agacttcaag ctcagcaccg gcacctgggt gcacgtcggc gcgctgcggg tgaagctgat
     3661 cgccgccggc gacccgctca tccaggacgc cgtgatcacc ggccacgatc gcgacgcggt
     3721 cggcgccctg ctgttcttga gccccgcggc caagggaatg gatgtcaaag agaagttgat
     3781 tcaaatactg aaaagcttcg aggaagaacc cagctccaca cgcatcactc gcctgatgat
     3841 catgtccgag ccgccgtcga tcgacgccaa cgagatcacc gacaagggct acatgaacca
     3901 gcgcgcggtg ctcgagcgtc gcgcagcgct ggtcgatcgt ctttattcag gtattgacgt
     3961 cgtaactact tcctagccag acccagccag gcgatggcgc cgacgacgat gaaggcgccg
     4021 acaatctgca ggggcgcgat ggcctggccg aggatgaccc agccgaggag gagcagcgag
     4081 atcggctcga agttcagcac caccgcatag ctggccgagc ccatcctggg cagcaccacg
     4141 aacaccgagg tgatcgccag gccgtagaac acgctaagca gcgccacccc catccagccg
     4201 gtggcatcgg cgggcgcacg gaaggccccc agcgcggcgc cgatcacagc gacgatcagg
     4261 gccaccgtag ccatggtcag cgccgagcgg atgcggccgt cgaccgcctt cagccagagg
     4321 tcggtcagga acagcgccgt ggcgaacgcg aacccggcgg caagagccca gagcacgccg
     4381 ggaccgaccg gcttgcccca gacgtcgagc gcgatcgaca ggccgacgag cgcgatcggc
     4441 atggcgatcg cggcgcgcgc gctcgggcgg ccgcggcccg cggcccaggt catcagcgag
     4501 agcatcaccg ggaacgtatt gaacgcgagc agcgccaggg ccaccgggat ctgcgacacc
     4561 gcagaataca ggcagtagct ctgcacggcg agggccacgc cgatgagcgc cgcccgcccc
     4621 aactgcgcgc gcgacagcgc gatcggcacg ccttgcaggc gcagcagcgc gaacacggcg
     4681 agcgaggtga acagcgagcg cgcgaacact ccggtcacca cgctcgcgcc gtggtcgaag
     4741 gcgatgcgcg ccgaaatatg gttggcgccg aaggtcacgg cgatcaccat caggatgacg
     4801 atccccagcc gcggtggtag cttcggttcc atgtctcccc tgaagaatgt acgcgtgatc
     4861 gagttctgcc agatcgccgc cggccccttc gccggcctgc tgctcgccga catgggagcc
     4921 gacgttatca aagtcgaatc gcccgaaggc gacggcatgc gaaactggcc gccgcatacc
     4981 gccggctatt ccgagaactt cgcctccctg aaccgcggca agaagtccgt ggtcctgaac
     5041 ctcaagaccc cgtccgacgt cgagaacgcg atccggctgt gcctgtccgc cgacgtcgtg
     5101 ctggagaact tccggcccgg cgtcatgaac cgcctgggcc tgggatatga ggttctgaaa
     5161 aatcaaaaac cggaaatcgt atattgctcc atctccgcct tcggccagga aggcccgcgc
     5221 tcgcaggaag gcggcttcga cgtcacgctg caggccatc
//
LOCUS       DASYJA010000263         5532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_402430, whole genome shotgun sequence.
ACCESSION   DASYJA010000263 DASYJA010000000
VERSION     DASYJA010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402430"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1684)
                     /locus_tag="VGO02_05635"
     CDS             complement(125..1684)
                     /locus_tag="VGO02_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7477205.1"
                     /translation="MRKLLFAVLLAVSLSANAVTLKWAAQNDILTLDPHSQNHATTHS
                     VLQYTYEGLTRYTRDYKIEPCLALSWKQMSDTQWRFNLRKDVKFHDGSPFTADDVVFS
                     FGRIKQPQGTNQVYVTGINEVRKVDDYTVDFILAGPTPILLRNIVDFRIMSKAWSEKN
                     RSQNVQDYAKKEEIFASRNTNGTGPYLIKSWEPDKQIVFAANKGWWGKLDGNVTDVVY
                     TPIKSDQTRVSALLSGDVDLVTDLPTQDVDRLRKDAKLKVLDGAEVRTIFLGMDQHND
                     ELKYSNVKGKNPFKDLRVRQALNMAIDREAIKRVTMRGLSIPAGLMIPPGVHGYDKKL
                     DAPFKYDIAGAKKLLAEAGYPDGIEFTLDCPNNRYVNDEKVCQAIVAMWAKAGIRAKL
                     NAMPFANYIPKILNFDTSVYMLGWGVATFDALYSLQSLLHTKTAGADGSFNLGRVSDP
                     KLDKTIDAIKITSDPKARDALLREGLMATRDQAYYVPLHHQLRPWAMKKNVSTVHKAD
                     DRPESRFARVN"
     gene            complement(1698..2876)
                     /locus_tag="VGO02_05640"
     CDS             complement(1698..2876)
                     /locus_tag="VGO02_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEV7477206.1"
                     /translation="MTSPVDRIRGYHAELREIRRDLHAHPELAFQESRTSSKVVEYLK
                     KWGIETHTGLAKTGVVGIIRNGKSDRGVGLRADMDCLPMHEASSFSHKSRYEGRMHAC
                     GHDGHTTMLLGAARYLAETRNFDGTAYLIFQPAEEGGGGGQVMVQEGLFDRFPANEVY
                     ALHNWPIMEPGTIAVRAGPMMAATDEIQITVRGKGGHAAVPQLTVDPVVAAAQVISAL
                     QTIASRNVNPLDAVVVSICSMQTSQVGAFNVIPEAVNLIGTVRSFRAETRELAERRVR
                     EICTKTAEALGGTAEVKYARGYPATVNSAKEAAFAARVGERIFGKANVVTDAEPTMGG
                     EDFSYMLQARPGAYVWLGQGGGPGGCFLHNPNFDFNDEVIPLGAGYLAALVEDALPAK
                     "
     gene            complement(2873..3844)
                     /locus_tag="VGO02_05645"
     CDS             complement(2873..3844)
                     /locus_tag="VGO02_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7477207.1"
                     /translation="MLAFVLRRLAQSVFVMLTVGLIAFSLFRFVGDPVIYMGGQDATD
                     EQRAQLRRDLGLDRPFYVQYAAFVAHAVQGEFGLSLRQVQPVSKLFVERLPATLELSL
                     VAALLALAAGIPMGIYTALRPHSPLSHVLLAISLAGVSLPTFLIGILLILVFSVQLGW
                     LPSFGRGDVVQLGWWTTGLLTASGLKSLILPAITLGLYQMTLIQRLVRSEMLEVLRTD
                     YIRFARARGLSDRAVHFGHALKNTLVPVITITGLQLGAIIAFAIITETVFQWPGMGLL
                     FIQAVSFADVPVMAAYLCLIALFFVLINLAVDLLYYAVDPRLRIQPT"
     gene            3865..4887
                     /gene="mutY"
                     /locus_tag="VGO02_05650"
     CDS             3865..4887
                     /gene="mutY"
                     /locus_tag="VGO02_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEV7477208.1"
                     /translation="MSRFASALVQWQRRHGRKGLPWQASTDPYRVWLSEVMLQQTQVA
                     AVIPYYERFLERFPTADALAAASEDEVLQLWSGLGYYARGRNLHAAAKLISQHGFPKT
                     AERIAELPGVGRSTAAAIAAFAFGERAAILDGNVKRVLARHAGIAGFPGERKVEAKLW
                     ALAESLLPKSSIETYTQALMDLGATVCIKAKPRCDACPVSKTCVALNKGLTEKLPVPR
                     PKKEVPSRSTTWLVLRHGGQVLLERRPSPGIWGGLWSFPEANGKDIARDCRNLGYRVS
                     SMRDLDEIQHGFTHFRLTIQPVLCEVSEMVLRAESPGRLWIDLAEAGGAATPAPVKKL
                     LQSFIL"
     gene            complement(5102..>5532)
                     /locus_tag="VGO02_05655"
     CDS             complement(5102..>5532)
                     /locus_tag="VGO02_05655"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEV7477209.1"
                     /translation="IREGAEVGEPAIPTQVGTLARITTWDMPQLGVLHVIAVGAQRFR
                     IKAHRVAPGGLARGDVELLDADADSPVPEHCVKCVKILQRVIGEQPDLLEPPHRVDSA
                     SWVGSRLTELLPLALSSKQELLELSDARARLERLNALLGR"
BASE COUNT          932 a         1773 c         1867 g          960 t
ORIGIN      
        1 agcagcgccg ccaccaccgt gaccggcgag cggcggaagc tccacgccag gtcgtggtcc
       61 cagagtcgcc tgagcaacac gccgcgcaac acgccccgca acacgccgcc cgaatgcgtc
      121 tggttcagtt gacgcgggcg aagcgcgatt ccggccggtc gtcggccttg tgcacggtgc
      181 tgacgttctt cttcatcgcc caggggcgca gctggtggtg cagcggcacg tagtacgcct
      241 ggtcgcgcgt ggccatcagc ccctcccgca gcagcgcgtc gcgcgctttg ggatcgctgg
      301 tgatcttgat tgcgtcgatg gtcttgtcga gcttcgggtc gctgactcgg ccgaggttga
      361 agctgccgtc ggcgccggcg gtcttggtgt gcagcagcga ctgcaggctg tagagcgcgt
      421 cgaaggtcgc cacgccccag cccagcatgt agacgctggt atcgaagttg aggatcttcg
      481 ggatgtagtt ggcgaacggc atggcgttca gcttggcgcg gatgccggcc ttggcccaca
      541 tcgcgacgat cgcctggcac accttctcgt cgttgacgta gcggttgttc gggcagtcga
      601 gggtgaactc gatgccgtcg gggtagccgg cctcggccag cagcttcttc gcgccggcga
      661 tgtcgtactt gaacggcgcg tcgagcttct tgtcgtagcc gtgcaccccc ggcgggatca
      721 tcaggccggc cgggatcgac aggccgcgca tggtgacgcg cttgatcgcc tcgcggtcga
      781 tcgccatgtt gagcgcctgc ctcacgcgca ggtccttgaa cgggttcttg cctttcacgt
      841 tcgagtactt gagctcgtcg ttgtgctggt ccatgccgag gaagatggtg cgcacctcgg
      901 cgccgtcgag gaccttgagc ttggcgtcct tgcgcagccg gtcgacgtcc tgcgtcggca
      961 ggtcggtgac caggtcgacg tcgcccgaga gcagcgccga gacgcgcgtc tggtcggact
     1021 tgatcggcgt gtagaccaca tcggtgacgt tgccgtcgag cttgccccac cagcccttgt
     1081 tcgccgcgaa cacgatctgc ttgtcgggct cccaggactt gatcaggtag gggccggtgc
     1141 cgttggtgtt gcgcgaggcg aagatctcct ccttcttcgc gtagtcctgg acgttctgcg
     1201 agcggttctt ctccgaccag gccttgctca tgatgcgaaa gtcgacgatg ttcctcagca
     1261 ggatcggcgt cggcccggcg aggatgaagt cgacggtgta gtcgtcgacc ttgcgcacct
     1321 cgttgatgcc ggtgacgtag acctggttgg tgccctgcgg ctgcttgatg cggccgaagg
     1381 agaacacgac gtcgtcggcg gtgaacggcg agccgtcgtg gaacttgacg tccttgcgca
     1441 ggttgaagcg ccactgcgtg tcggacatct gcttccagct cagcgccagg cagggctcga
     1501 tcttgtagtc cctggtgtag cgcgtgagcc cttcgtaggt gtactgcagg accgagtgcg
     1561 tggtggcgtg gttctgcgag tgcgggtcga gcgtcaggat gtcgttctgc gcggcccatt
     1621 tcagcgtaac ggcgtttgca gacaaactga ctgctagcag aactgcaaaa agaagctttc
     1681 tcactcgatc ccccgtctta tttggccgga agcgcgtcct cgaccagcgc cgcgaggtag
     1741 ccggcgccca gaggaatcac ctcgtcgttg aaatcgaaat tcggattgtg caggaagcag
     1801 ccgccgggcc cgccgccctg gccgagccag acgtaggcgc ccgggcgcgc ctgcagcatg
     1861 taggagaagt cctcgccgcc catggtcggc tcggcgtcgg tgacgacgtt ggccttgccg
     1921 aagatacgct cgccgacgcg cgccgcgaac gccgcctctt tggccgagtt gaccgtcgcc
     1981 gggtagccgc gcgcgtactt gacctcggcc gtgccgccaa gcgcttccgc ggtcttggtg
     2041 caaatttccc tgacacgtct ttcggccagc tcgcgcgtct cggcgcggaa gctgcgcacc
     2101 gtgccgatca ggttgacggc ctcggggatc acgttgaatg cgccgacctg gctggtctgc
     2161 atcgagcaga tgctgaccac caccgcgtcg agcgggttga cgttgcggct ggcgatggtc
     2221 tgcagggccg agatcacctg cgccgcggcg accaccgggt cgacggtgag ctgcggcacc
     2281 gcggcgtggc cgcccttgcc gcgcaccgtg atctggatct cgtcggtcgc cgccatcatc
     2341 ggcccggcgc gcaccgcgat cgtgcccggt tccatgatcg gccagttgtg cagcgcgtac
     2401 acctcgttcg ccgggaaacg gtcgaacagg ccttcttgca ccatcacctg gccgccgccg
     2461 ccgccttctt ccgccggctg gaagatgagg taggcggtgc cgtcgaagtt gcgcgtctcg
     2521 gcaaggtagc gcgccgcgcc gagcagcatg gtggtgtggc cgtcatgccc gcaagcgtgc
     2581 atccgccctt catatcttga tttatgagaa aaactgctgg cttcgtgcat cggcaggcag
     2641 tccatgtcgg cgcgcaggcc gacaccacga tcggatttcc cgttgcgaat gatcccaacc
     2701 acccccgttt tcgccaggcc ggtgtgcgtc tcgatgcccc atttcttcag gtactcgacc
     2761 accttcgacg aggtgcggct ctcctggaag gcgagctccg gatgggcgtg cagatcgcgg
     2821 cggatctcgc gcagctcggc gtgatagccg cggatcctgt cgacggggct ggtcatgtgg
     2881 gttggatgcg aagccttggg tcgaccgcat agtaaagcag gtcgaccgcg aggttgatga
     2941 gcacgaagaa tagcgcgatc aggcacaggt aggccgccat gaccggcaca tcggcgaaac
     3001 tcaccgcctg gatgaacagc agccccatgc cgggccactg gaacacggtc tcggtgatga
     3061 tggcgaaggc gatgatcgcg ccgagctgca gcccggtgat ggtgatcacc ggcacgagcg
     3121 tgttcttcag cgcgtggccg aagtgcaccg cgcggtcgct gagtccgcgg gcgcgcgcga
     3181 agcggatgta gtcggtgcgc agcacttcga gcatctccga gcgcaccagc cgctggatca
     3241 gcgtcatctg gtacaagccg agggtgatcg cgggcaggat cagcgatttc aggcccgacg
     3301 cagtgagcag gcccgtggtc caccagccga gttgcaccac gtcgccgcgg ccgaaggagg
     3361 gcagccagcc gagctgcacc gagaacacca ggatcagcag gatgccgatg agaaaggtcg
     3421 gcagcgagac gcccgcgagg gatatcgcca acaatacgtg tgacagcggc gagtgcggcc
     3481 gcagagcagt ataaattccc atcggaatgc cggctgccaa cgcgagcagg gcagcgacga
     3541 gcgatagttc gagcgtcgcc ggcagccgct cgacgaacag cttcgacacc ggctggacct
     3601 gccgtaggct gaggccgaac tcgccctgca ctgcatgggc gacgaacgcc gcgtactgca
     3661 cgtagaaagg ccggtcgagg ccgaggtcgc ggcgcagctg cgcgcgctgc tcgtcggtcg
     3721 cgtcctgccc tcccatgtag atcaccgggt cgccgacgaa gcggaacagc gagaacgcga
     3781 tcagcccgac ggtgagcatc acgaaaaccg actgggcgag ccgccgcagg acgaatgcca
     3841 gcatgcgtcc attagaatcc ggttgtgtcg cgattcgcaa gcgccctcgt gcaatggcag
     3901 cgccgccacg gccgcaaggg cctgccgtgg caggcgagca cggaccccta ccgcgtctgg
     3961 ctgtccgagg tgatgctgca gcagacccag gtcgccgcgg taatccccta ctacgagcgt
     4021 ttcctcgaga ggttccccac ggctgacgcg ctcgccgcgg cgtcggagga tgaagtcctg
     4081 cagctctgga gcggtctggg gtattacgcg aggggcagga atctccacgc ggctgccaag
     4141 ctgatctctc agcacggctt cccgaaaacc gccgaaagga tcgccgagct gccgggcgtc
     4201 ggccgctcca ccgctgccgc catcgccgcc ttcgctttcg gcgagcgcgc cgcgatcctc
     4261 gacggcaacg tcaagcgcgt gctcgcacgc cacgccggca tcgccggctt tcccggcgag
     4321 aggaaggtgg aagcgaagct gtgggcgctc gccgaaagcc ttctgccgaa gagcagcatc
     4381 gagacctata cgcaggccct gatggacctt ggcgcgaccg tctgcatcaa ggcgaagccc
     4441 cgctgcgacg cgtgtccggt cagcaagacc tgcgtcgcgc tgaacaaagg gctaaccgag
     4501 aagctgcccg taccgcgacc gaagaaggaa gtgccttcaa gaagcacgac ctggttagta
     4561 ctgcggcatg gcggccaggt gctgctcgag cggcgaccga gcccgggtat ctggggcggc
     4621 ctttggtcct tccctgaagc aaatggaaag gacatcgccc gggattgccg caatctcggc
     4681 taccgcgtca gttcgatgcg agacctggac gagatccagc acggcttcac ccacttccga
     4741 ctcacgattc agccagtgct ctgcgaggtg agcgaaatgg tgctgcgcgc cgaatcgccg
     4801 ggccgccttt ggatcgacct tgcagaagcc ggtggtgccg cgactccggc gccggtcaaa
     4861 aagctgctgc agagctttat cctgtagtaa atcgggctat gatggggtga gcggcatggt
     4921 gccgcgatcc aagccaactg actaacctca atccgctgaa gtactgcttc cacggccagc
     4981 actcgcagcc gcgcgtcacc ttccgcacgc tgccgggcaa gacccgccgc caggtctgcg
     5041 ccgagtgcta cgccaagatc aaagccgacc ggctgtcgaa gaagaacgcc gccaagcttc
     5101 cctagcggcc gagcagcgcg ttcagtcttt ccagccgggc acgcgcatcc gatagctcga
     5161 gcagttcctg cttggaagac agcgcaagcg gcaggagctc ggtgaggcgc gagccgaccc
     5221 acgaggcgga gtcgacgcgg tgcggcggct cgagcagatc gggctgctcg ccgatgacgc
     5281 gctgcagaat cttgacgcac ttcacgcagt gctcggggac gggggagtcc gcgtcggcat
     5341 ccagcagctc gacgtcgccg cgggcgaggc cgcccggcgc gacgcgatgc gccttgatgc
     5401 ggaagcgctg cgcgccgacg gcgatcacgt ggagcactcc gagctgcggc atgtcccagg
     5461 tggtgatgcg cgcgagggtg ccgacttggg tcgggatggc gggctcgccc acttcggcgc
     5521 cttcacggat ca
//
LOCUS       DASYJA010000264         3503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2672727, whole genome shotgun sequence.
ACCESSION   DASYJA010000264 DASYJA010000000
VERSION     DASYJA010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2672727"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="VGO02_05660"
     CDS             <1..932
                     /locus_tag="VGO02_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV7477210.1"
                     /translation="FGRTWQVLMSAAPQYRDRPEDIGKVWVRSAEGPSAGSMIPLDSL
                     AKVRYSSGPDSLDRFNNLPAVKIFGQGAPGVSSGQAIRAVEELAAKVLPQDFSYDWGG
                     VSYQEKRSAGTSGLALGLAVVMVFLILAAQYEKWSLPLSVLLAMPFGTFGALAAIWLR
                     GLTNDVYFQIGLVTLLGLAAKNAILIVEYAVMKHEQGLSASAAAVEAARLRFRPILMT
                     SLAFILGVMPLAFSTGAGAGARVSAGTGVMGGMLAATFLAIFFVPMFFKLMTDRHLTE
                     KRSTADIKHEAEMARNITHKPVQTAHHAPHRHE"
     gene            937..2307
                     /locus_tag="VGO02_05665"
     CDS             937..2307
                     /locus_tag="VGO02_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEV7477211.1"
                     /translation="MLIGFLVLSGCVSPVNYERPPVDLPDAWKESAPRYAEDGRWWRI
                     YQDPDLDKVVDEALLGNSDLVVALARVDEARGLLGEANSFLFPSVDAQAVASRQQVST
                     RTATAFPGIPREYNNHRATLNVSYELDLFGRLRAGKAAARAELEASEASRDAVRLALA
                     AQAAKSYFALRALDEQVDLTRRTVRLREDALALQRKRNAAGVISEFELRQLEAEAAAV
                     RAQLPPLERDREREEAALSVLLGRTPREVFSGTVRVRQAFDETPEAPAVPSGMPSELL
                     LRRPDLVEAERQLAAANARVAVARSEMFPSISLTAFFGSESAALANLFAGGSAATWQV
                     AAGLTQPIFAGGRLAARSDAAKARERQALAQYEQAIRSAFGDVRTALVAQMRSRESYE
                     AETTRANALNETLRFARLRYENGVASQLDVLDAERGLLAARSARIEALRAHRAAVADL
                     FRALGG"
     gene            complement(2312..3094)
                     /locus_tag="VGO02_05670"
     CDS             complement(2312..3094)
                     /locus_tag="VGO02_05670"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEV7477212.1"
                     /translation="MTPRLPSVFISHGSPMHALEPGAAGEAWRSLGRRLGKPKAIVIA
                     SAHWETNLPMLTGSAKPETIHDFYNFPEPLYRLRYAAPGAPQVAQQAQALLKSAGFTA
                     AIDGTRGLDHGAWSPLLYMYPDADVPVVELAVQPALGTRHHLLLGKTLRPLADESVLL
                     VGSGHMTHNLRDWARGAGQPQPYAVNFQRWVHDRIEAHDHDSLAEYRSLSPDGVRAHP
                     TDEHFLPLFVALGAAPEKTRPERVYDDIESGVLAMDAYVFGN"
     gene            complement(3091..>3503)
                     /locus_tag="VGO02_05675"
     CDS             complement(3091..>3503)
                     /locus_tag="VGO02_05675"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEV7477213.1"
                     /translation="PVILQYLADQKPDAGLAPKPGDKARYKLQEALNFVTSELHKGMG
                     NFFNPAMTEEWRKAVTDRLSVRLDWLAKQLEGKQYFMGDKFSVADAYLFTILNWAGPS
                     KFDLSKWPAITDYQKRVAARPKVQEALKAEGLVK"
BASE COUNT          502 a         1211 c         1223 g          567 t
ORIGIN      
        1 agttcggccg cacctggcag gtgctgatgt cggccgcgcc gcaataccgc gaccgtcccg
       61 aggacatcgg aaaggtctgg gtccgctccg cggagggtcc gagcgcaggc tcgatgattc
      121 cgctcgactc gctcgccaag gtgcgctaca gctccggacc cgactcgctc gaccgcttca
      181 acaacctgcc ggcggtgaag atcttcggcc agggcgcgcc gggggtgtct tcggggcagg
      241 cgatccgggc ggtcgaggag ctcgcagcca aggtgctgcc gcaggacttc agctacgact
      301 ggggcggcgt ctcgtaccag gagaagcgct cggccgggac ctcgggcctt gcgctcggcc
      361 tcgccgtcgt catggtgttc ctcatcctcg ccgcgcaata cgagaagtgg tcgctgccgc
      421 tctcggtgct cctggcgatg ccgttcggca cgttcggggc gctcgcggcc atctggttga
      481 ggggtttgac aaacgacgtg tacttccaga tcgggctggt cacgctgctc ggcctggcgg
      541 cgaagaacgc catcctgatc gtcgagtacg cggtgatgaa gcacgagcag ggactgtcgg
      601 catcggccgc cgcggtcgag gccgcgcggc tgcgcttccg gccgatcctg atgacctcgc
      661 tcgccttcat cctcggcgtg atgccgctcg ccttctcgac cggcgccggc gccggcgcac
      721 gcgtttcggc cggcacgggt gtcatgggcg gcatgctggc ggcgaccttc ctcgcgattt
      781 tcttcgtgcc gatgttcttc aagctgatga ccgaccggca cctgaccgag aaacgctcca
      841 ccgccgacat caagcacgag gccgagatgg cgcgcaacat cacccacaag cccgtgcaga
      901 ccgcgcacca cgccccgcac cgccatgagt agatttttgc tgataggttt tttggttctt
      961 tcaggctgcg tttcaccggt caactacgag cgcccgccgg tcgacctgcc cgacgcctgg
     1021 aaggagagcg cgccgcgcta cgccgaggat ggccgctggt ggcgcatcta ccaggatccg
     1081 gacctcgaca aggtggtcga cgaggcgctc ctcggcaact ccgacctggt ggttgcgctt
     1141 gcgcgcgtcg acgaggcccg cggcctgctc ggcgaggcga actcgttcct cttcccgagc
     1201 gtcgatgcgc aggccgtggc cagccgccag caggtctcga cgcgcaccgc gaccgcgttt
     1261 cccggcatcc cgcgcgagta caacaaccat cgcgcgacgc tgaatgtctc ctacgagctc
     1321 gacctcttcg ggcggctgcg cgccggcaag gcggcggcgc gtgccgagct cgaggcgagc
     1381 gaggcctcgc gcgacgcggt gcgcctggcg ctcgcggcgc aggcggccaa gtcctatttc
     1441 gcgctgcgcg cgctcgacga gcaggtggat ctgacgcggc gcacggtgcg gctgcgcgag
     1501 gatgcgctcg ccctgcagcg taagcgcaac gccgccggcg tgatctcgga gttcgagctg
     1561 cgccagctcg aggccgaagc cgccgcggtg cgtgcacagc tgccaccgct cgagcgcgac
     1621 cgcgagcgcg aggaagcagc actctcggta ttgcttggac gcacgccgcg ggaggtattt
     1681 tcgggaaccg tgcgcgtgcg gcaggccttc gacgagaccc ccgaggcgcc ggcggtgcct
     1741 tcgggcatgc cctcggagct gctgctgcgc cggccggacc tggtcgaggc cgagcggcag
     1801 ctcgccgccg ccaacgcgcg cgtcgccgtg gcgcgcagcg agatgttccc ctcgatcagc
     1861 ctgaccgcct tcttcggcag cgagagcgcg gcgctcgcca acctgttcgc aggcggctcg
     1921 gccgcgacct ggcaggtcgc ggccggcctc acccagccga tcttcgccgg cggccgcctc
     1981 gccgcgcgct ccgacgcggc caaggcgcgc gagcgccagg ccctcgcgca gtacgagcag
     2041 gcgatccgct cggcgttcgg cgacgtgcgc accgcgctcg tcgcccagat gcgctcgcgc
     2101 gaaagctacg aggccgagac cacccgcgcg aatgcgctca acgagaccct gcgtttcgcg
     2161 cgcctgcgct acgagaacgg cgtcgccagc cagctcgacg tgctcgacgc cgagcgcggc
     2221 ctgctcgccg cgcgcagcgc gcgcatcgag gccctgcgcg cgcatcgcgc ggcggtcgca
     2281 gatctgttca gggcattggg cggctagcgg ttcagtttcc gaacacgtag gcgtccatcg
     2341 ccagcacgcc gctctcgatg tcgtcgtaga cgcgctcggg ccgtgttttc tcaggcgcgg
     2401 cgcccagggc gacgaacagc ggcaggaaat gctcgtcggt gggatgcgct cgcacgccgt
     2461 ccggcgagag cgagcggtac tcggcgaggc tgtcgtggtc gtgcgcctcg atcctgtcgt
     2521 gcacccagcg ctggaaattc accgcatagg gctgaggctg ccccgcgccg cgcgcccagt
     2581 cgcgcaggtt gtgggtcatg tggccggagc cgacgagcag caccgattcg tcggccagag
     2641 gtctcagggt ctttcccaaa agcaggtgat gccgcgtacc gagcgccggc tgcacggcga
     2701 gctcgaccac cggcacgtcg gcgtcgggat acatatagag gagcggcgac caggcgccgt
     2761 ggtccaggcc gcgggtgccg tcgattgcag cagtgaagcc ggctgatttc agaagcgcct
     2821 gcgcctgctg cgcgacttgc ggcgcgccgg gcgccgcgta gcggaggcgg tacagcggct
     2881 cggggaaatt gtagaagtcg tggatcgtct ccggcttcgc gctgccggtg agcatcggca
     2941 ggttggtttc ccagtgcgcc gaggcgatga cgatcgcctt cggcttgccg aggcggcggc
     3001 cgagcgagcg ccacgcctcg cccgccgcgc cgggctcgag cgcgtgcatg ggcgagccgt
     3061 gggaaatgaa gacgctcggc agccgaggcg tcacttcacc agcccttcgg ccttcagcgc
     3121 ctcctgcacc ttgggccgcg ccgcgacgcg cttctgatag tcggtaatcg ccggccactt
     3181 cgacaggtcg aacttggacg gccccgccca gttgaggatg gtaaacaggt aggcgtcggc
     3241 gacgctgaac ttgtcgccca tgaagtactg ctttccttcg agctgcttgg ccagccagtc
     3301 gagccgcacg ctgaggcgat cggtcaccgc tttgcgccat tcctcggtca tcgccggatt
     3361 gaagaagttg cccatgcctt tgtgcagctc ggaggtgacg aagttgagcg cctcctgcag
     3421 cttgtagcgc gccttgtcgc cgggcttggg cgcgagaccc gcgtcgggct tctggtcggc
     3481 caggtactgc aggatcaccg ggc
//
LOCUS       DASYJA010000265         2860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2036633, whole genome shotgun sequence.
ACCESSION   DASYJA010000265 DASYJA010000000
VERSION     DASYJA010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2036633"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VGO02_05680"
     CDS             <1..552
                     /locus_tag="VGO02_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEV7477214.1"
                     /translation="RDELGFFNNSKEVERLAASVLDSGDVYIVPAFTGLGAPYWDANA
                     RGTIVGITRGTSRAHLARAALEAIAFQSADVLEAMQQDAASPLTELRVDGGAAANDLL
                     MQFQADLLGVPVVRPKILETTALGAAYLAGLTTRLWKSREELAAQWQAERRFEPRMER
                     KDAEARMLRWRDAVSRSRNWTAG"
     gene            complement(549..1451)
                     /locus_tag="VGO02_05685"
     CDS             complement(549..1451)
                     /locus_tag="VGO02_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477215.1"
                     /translation="MNAPEKIKQYDRDAQAVGNIVHLEHFNVVIPDQRLATLFYVVGL
                     GGTRDPYLFTGLENMWVNFGKTQVHLPSRNVPPRAEVLRGTAGFVVPSLADLEKRLTH
                     AGNEMKRVAPDLPNKFSFKKQKDFIEATCPWGNRVRCHEPAAEFGSAQIGLMYVDFDV
                     PPGTAEGIARYYNEVMRAPARLEKKDRAVVGVGRAQRLMFTETAAPQPEYDNHHVQIY
                     IADFGTPYHWLKERDLITMETDANEWRFQWIVDPKDGRKLFQIEHEVRSMKHRLFGRP
                     LVNRNHGISNMTYEANSDAFRGTV"
     gene            complement(1456..1986)
                     /locus_tag="VGO02_05690"
     CDS             complement(1456..1986)
                     /locus_tag="VGO02_05690"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEV7477216.1"
                     /translation="MLHKRKGNPFTEGSRELFDLAGQGRIDVFFLGGAQIDGAANINL
                     VRAEGRRFPGSFGSAFMYPMVPRVILFREEHSKRTLVPKVEFVSAAGNPQALLTGKAL
                     FSWQKDRRRFRLESVHPGHTADEVRNDTGFDFDFQQISTTKEPSGQELALLRGPVAKA
                     IAADYPDFAKRVWGLN"
     gene            complement(2085..>2860)
                     /locus_tag="VGO02_05695"
     CDS             complement(2085..>2860)
                     /locus_tag="VGO02_05695"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEV7477217.1"
                     /translation="MPPDYSMPAMEVVRELIRRKSRNLKLLGVPVLGLCADLLIGAGC
                     VAEVESSAVSLGEAGLAPRFSEAVEKGTLTVTDATCPAIHTGLQATEKGVPFMPIGGV
                     LGSDLVKGRKDWKVGPDPFSGKEFLLVKAIQPDVALFHARWADEAGNVWVGRRKELAT
                     IAHAARLTFVSFEEMRQGSMLDDELFAPGVISATYVSATAHVPNGAWPLGIADVYGID
                     DAHLALYAKAARTREGFEQYLKEFVWSQKKSSSPASSPA"
BASE COUNT          454 a          931 c          954 g          521 t
ORIGIN      
        1 cgcgacgaac tgggtttctt caacaactcg aaggaagtgg agaggctggc ggcaagcgtg
       61 ctcgacagcg gcgacgtcta catcgtcccc gccttcaccg gcctcggcgc gccctactgg
      121 gacgcaaacg cacgcggcac gatcgtcggc atcacccgcg ggacttcccg cgcgcatctt
      181 gcgcgcgccg ccctggaagc gatcgccttc cagagcgccg acgtgctcga ggcgatgcag
      241 caggacgcgg cctcgccgct caccgagctg cgcgtcgacg gcggcgccgc ggcgaacgac
      301 ctgctgatgc agttccaggc cgacctgctg ggcgtgccgg tggtgcggcc gaagatcctc
      361 gagaccacgg cgctcggcgc cgcctacctg gccgggctga cgacccgcct gtggaaatcg
      421 cgcgaggagc tggccgcgca gtggcaggcc gagcggcgct tcgagccgcg gatggagcgc
      481 aaggacgccg aagcgaggat gcttcgttgg cgggatgccg tcagccgttc caggaactgg
      541 acagcgggtt agaccgttcc cctgaaggcg tcggagttcg cttcgtaagt catgttgctg
      601 atgccgtggt tgcggttcac cagcggccgg ccgaacaggc ggtgcttcat gctgcgcacc
      661 tcgtgctcga tctggaacag cttcctgccg tccttcgggt cgacgatcca ctggaagcgc
      721 cactcgttgg cgtcggtttc catggtgatc aggtcgcgct ccttcagcca gtggtacggc
      781 gtgccgaagt cggcgatgta gatctgcacg tggtggttgt cgtactcggg ctgcggcgcc
      841 gcggtctcgg tgaacatcag gcgctgggcg cggccgacgc ccaccacggc gcgatccttc
      901 ttctcgaggc gcgcaggcgc gcgcatcact tcgttgtagt agcgggcgat gccctccgcc
      961 gtgcccggcg gcacgtcgaa gtcgacatac atcaggccga tctgcgcgct gccgaactcg
     1021 gctgccggct cgtggcagcg cacgcggttg ccccacgggc aggtcgcctc gatgaaatct
     1081 ttttgttttt tgaagctgaa tttgttgggc agatccggcg cgacccgctt catctcgttg
     1141 ccggcatgcg tcaggcgctt ttccaggtcg gccaggctgg gcaccacgaa accggctgtg
     1201 ccgcgcagca cttcggcgcg cggcggcacg ttgcggctcg gcaggtgcac ctgggtcttg
     1261 ccgaagttga cccacatgtt ctccaggccg gtgaacaggt acggatcgcg cgtgccgccg
     1321 aggccgacga cgtagaacag cgtcgccagg cgctggtcgg ggatgacgac gttgaagtgc
     1381 tcgaggtgga cgatgttgcc caccgcctga gcgtcgcggt cgtattgctt gatcttctcg
     1441 ggagcgttca tggcgctagt ttaatcccca gacccgtttg gcgaaatcgg ggtagtcggc
     1501 ggcgatggcc ttcgccaccg ggcctctgag cagtgcaagc tcctgccccg acggttcttt
     1561 tgtagtgctt atttgttgaa aatcaaaatc aaacccggtg tcgtttcgca cctcgtcggc
     1621 cgtatgcccg ggatggacgc tctccagccg gaagcggcgg cggtcctttt gccagctgaa
     1681 gagcgccttg ccggtgagca gcgcctgcgg gttgccggcg gccgagacga attccacctt
     1741 cggcaccagc gtgcgcttcg aatgctcctc gcggaacagg atcacgcgcg gcaccatcgg
     1801 gtacatgaag gccgagccga acgagccggg aaagcgccgg ccctccgcgc gcaccaggtt
     1861 gatgttggcc gcgccgtcga tctgcgcgcc gccgaggaag aacacatcga tgcggccctg
     1921 cccggcgagg tcgaacagct cgcgcgagcc ttccgtgaac gggttgcctt tgcgcttgtg
     1981 cagcatcgat acgcgcaagg acgggttatt cagtttgtat aaagaaacac ctgttgcggg
     2041 gatcggcgac gccgccccga cggccacatg ctgcgccccg tcgatcaggc gggcgatgac
     2101 gccggcgagg agctcttctt ttgcgaccag acgaattcct tcaggtactg ctcgaatccc
     2161 tcgcgagtcc tggcggcttt cgcgtaaagc gcgagatgtg cgtcgtcgat gccgtagacg
     2221 tcggcgatgc cgagcggcca ggcgccgttc ggtacgtgcg ccgtcgcgct cacataggtc
     2281 gcgctgatga ctcccggcgc gaacagctcg tcgtcgagca tgcttccttg cctcatttcc
     2341 tcgaagctca cgaacgtcag gcgggcagca tgcgcgatcg tcgccagctc cttgcgacgc
     2401 ccgacccaca cgttgccggc ttcgtcggcc cagcgcgcat ggaacagcgc gacgtcgggc
     2461 tggatggctt tcacgagaag aaactctttg ccggaaaacg gatcaggacc caccttccag
     2521 tccttgcggc ctttgacgag gtcggacccg agcacgccgc cgatgggcat gaacggcacg
     2581 cctttctcgg ttgcctgcag cccggtgtgg atcgcggggc aggtcgcgtc cgtgaccgtg
     2641 agggtgccct tctcgacggc ctccgagaag cgcggcgcga gtcccgcctc gcccagcgac
     2701 actgcgctcg actcgacttc ggcgacgcag cccgcgccga tgagcaggtc tgcgcacagg
     2761 cccagtaccg gcactccaag aagcttcaga tttctggatt tacggcggat cagctcgcgc
     2821 acgacctcca tggccggcat ggagtagtcc ggcggcatgg
//
LOCUS       DASYJA010000266         7320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2036636, whole genome shotgun sequence.
ACCESSION   DASYJA010000266 DASYJA010000000
VERSION     DASYJA010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7320
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2036636"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1485)
                     /locus_tag="VGO02_05700"
     CDS             complement(<1..1485)
                     /locus_tag="VGO02_05700"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEV7477218.1"
                     /translation="MSGGNGNEARFERLLAYLKTSRGFDFGGYKRPSLTRRIQHRMDL
                     LGVPGFDEYLDHLEVHPGEFAELFDSILINVTAFFRDAPAWEYLAAEVLPAMLRAKTG
                     GEPVRVWSAGCATGEEAYSIAMLLAEALGVEAFLERVKIYATDLDDQALATARAAAYA
                     ARDLKDVAPQLQERYFQPQGTRQVFRADLRRAVIFGRHDMMSDSPIGGLDLILCRNTL
                     MYFNADVQARILSRFHFALNNGGYLFLGKAEMLLTRGTLFQPLDLKSRIFSKVTQPGE
                     SRRRVSVLPPPPAAGAGERGGRLLDQSSEESPVARIVVDENGVLALVNQRARVLFRID
                     PGDVGRLLHDLELSYRPAELRSLIERAYGERRPIAQTNVERRLDDGSVQMLDILVAPL
                     FDDQGVPLGVAITFLDVSYVQELKDELRRSREELQTTNEELQSSNEELETTNEELQSS
                     NEELETTNEELQSTNEELETMNEELQSTNEELQTVNDQLRQSTDE"
     gene            complement(1482..2066)
                     /locus_tag="VGO02_05705"
     CDS             complement(1482..2066)
                     /locus_tag="VGO02_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEV7477219.1"
                     /translation="MPDGFEIVALAASAGGLRALSEVLAGLPANFPAALVVVQHLDPR
                     HRSLMAEILGRRTPLAVRQAADDEHVQPGSVFIAPPDRHLLVNPDGLLSLTRTELVHF
                     SRPSADLLLESVAASYRERAIGVILSGSGSDGAMGAKAIKKMGGTLIVQDPATAEFSG
                     MPGAARQTGLADFVLPLEEISGALRTLLGERRAA"
     gene            complement(2082..2609)
                     /locus_tag="VGO02_05710"
     CDS             complement(2082..2609)
                     /locus_tag="VGO02_05710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV7477220.1"
                     /translation="MTQVDSFSLFSSVKVVRELLQRKLEDGTLHAQGELEAGLQEIEA
                     LWHELRERSDELASERQRYTEFFEYAPDAYLVTDSHGTIREANRAAAELLGIAARALD
                     GKPLVSYVPEAERRDFRAHVVRASAQPDGEVSAWRGALTPAQGQPVAAHFRVRAMPVP
                     NGGLAALCWLVRRGD"
     gene            complement(2610..3116)
                     /locus_tag="VGO02_05715"
     CDS             complement(2610..3116)
                     /locus_tag="VGO02_05715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV7477221.1"
                     /translation="MAGTDLKRLKGRLAVAHADVEGRPSARELEIAVEEIEALWDELG
                     RHADRLASERERNAALFDRAPFACLITDIHGNVHEANLAAVALLDVPAGYLFGKPLAI
                     FIADEEREAFRMHLAMAVRDPHPIEGWLSRIKSPDGQPREVKIDLRPMPPDAENFLPL
                     LWFLRDIE"
     gene            3250..4563
                     /locus_tag="VGO02_05720"
     CDS             3250..4563
                     /locus_tag="VGO02_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEV7477222.1"
                     /translation="MKISVLGLGYVGAVSAGCLARDGHEVIGVDPERVKVDLINEGRS
                     PIIEKDIGGIIAEAVAAGRLAASTDLEAAVRHTDLALVCVGTPSMPNGGLDLRYVRRV
                     CEQIGKTLAAHHGAPVIVMRSTMLPGSMREVVIPTLEKFSGKRAGEEFGVCINPEFLR
                     EGTAVHDYYHPPKTVIGEVNRASGDLLANLYGKMPGPMIRTDIETAEMVKYADNPWHA
                     LKVGFANEIGTLCKALQVDAHRVMEIFCQDLKLNLSPYYLKPGFAFGGSCLPKDVRAL
                     LHKAKTLDLNLPILASILPSNQLQIERGIQAVVDKTNRKVGILGFSFKAGTDDLRESP
                     MVELTERLIGKGYDLRVYDRNVSLAALHGANRDYILNRIPHISRLMVPTIDEVLAHAE
                     TIVIGNAAPEFAEVPKRIGDGQTVIDFVRVCDSRTVLGVYEGLCW"
     gene            4595..4849
                     /locus_tag="VGO02_05725"
     CDS             4595..4849
                     /locus_tag="VGO02_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477223.1"
                     /translation="MRTFFVRPERDGGYGASDGTTYDNAWNGLENVDWQAMAAGDPAQ
                     LWLCGGPGGPGGFMTVFVEWSYFETAPQALRRQAWSATVG"
     gene            4828..5793
                     /locus_tag="VGO02_05730"
     CDS             4828..5793
                     /locus_tag="VGO02_05730"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV7477224.1"
                     /translation="MVGYRRMILNAVALVPGAAQLPPVRSRLQKRGVGTGGTNDARYC
                     YTVWLRHLVTAARLGLNPDPREIAELGPGDSIGAGLAALLCGAERYSALDLVSHANPV
                     SNVLIFDQLVELFRARADLPGDGDYPNISPRLDDYRFPAQILDEARMQRALHPERVAR
                     LRDAVLGDDPSGLIRYRVPWQEESVLERGSQDLVFSQAVLEHIDALAEAYRAMRLWLK
                     PSGHVSHQIDFKCHDLADTWDGHWRYGDLRWTMLRGRDSWSINRQPYSEHLRLLEQTG
                     FEVLAAHPVQREPSYPRSSLARRFSAMTEADRGVSGVYLVARPVL"
     gene            5805..7058
                     /locus_tag="VGO02_05735"
     CDS             5805..7058
                     /locus_tag="VGO02_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV7477225.1"
                     /translation="MRQRRALFLVENLPSPFDRRVWQEATALRDAGYLVSIICPTGKG
                     YEKTFEALDGIEIFRYRLAEADGVAGYLLEYSAALAFTFALSWKVFFRQGFDVIHACN
                     PPDLFFLIGGFWKLFGKKFLFDHHDASPELYEAKFGRRGLLWKAMMLLEKLTFRTADV
                     SLATNESYKRIAVTRGGMPPEKVFVVRSGPSLERMKIGFHDEKLKKGKKYLVGYVGVM
                     GRQEGIDYLLRAAAHIVHDLGRKDVHFGLVGGGTSLEEMKRLADELGLNDCVTFTGRV
                     SDEQMLAMLNTADVCVNPDAANEMNDISTMNKVMEYMALGKPMVQFDLAEGRFSAQRA
                     SLYARRNNAFDLAAKIVELLDNPEKRAAMGEIGRRRVLEELEWRHEVPKLLQAYEALW
                     APAKPRPASPAARKGPAWLSGGRGR"
     gene            7031..>7320
                     /locus_tag="VGO02_05740"
     CDS             7031..>7320
                     /locus_tag="VGO02_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477226.1"
                     /translation="MAKRRTWALIALAGAALFGAYSAQRSADELLVAGAGASAPSRSS
                     ATIPGNLPERDILHAQKADLFAPPYVAPPPPPPPPPPPLAAAAEQPSNPYRFA"
BASE COUNT         1146 a         2486 c         2533 g         1155 t
ORIGIN      
        1 ctcgtcggtg ctctggcgca gctggtcgtt gacggtctgc agctcctcgt tggtggactg
       61 cagctcctcg ttcatcgtct cgagctcttc gttggtcgac tgcagctcct cgttggtggt
      121 ctcgagctcc tcgttggagg actgcagctc ctcgttggtg gtctccagct cctcgttgga
      181 ggactgcaat tcctcgttgg tggtctgcag ctcctcgcgc gagcggcgca gctcgtcctt
      241 cagctcctgc acgtagctca cgtcaaggaa agtgatcgcc acgccgagcg gcacgccctg
      301 gtcgtcgaac aggggcgcca ccaggatgtc gagcatctgg acgctgccgt cgtcgaggcg
      361 ccgctcgacg ttggtctgcg cgatcgggcg gcgctcgcca taggcgcgct cgatgagcga
      421 gcgcagctcg gccggccggt acgagagctc gaggtcgtgc agcaggcggc cgacgtcgcc
      481 cgggtcgatg cggaacagca cgcgcgcgcg ctggttgacg agggcgagca ctccgttctc
      541 gtcgaccacg atgcgcgcca ccggcgattc ctcgctcgac tggtcgagca ggcgcccgcc
      601 gcgctcgccg gcgccggcgg cgggcggcgg cggcagcacc gagacgcgcc ggcggctctc
      661 gccgggctgc gtcaccttgg agaagatgcg cgacttgagg tcgagcggct ggaacagggt
      721 gccgcgcgtc agcagcatct ccgccttgcc gaggaagagg taaccgccgt tgttgagcgc
      781 gaagtggaag cgcgagagta tccgtgcctg tacgtcggcg ttgaagtaca tcagcgtgtt
      841 gcggcacagg atcaggtcga ggccgccgat cggcgagtcg ctcatcatgt cgtggcggcc
      901 gaagatcacc gcgcgccgca ggtcggcgcg gaacacctgg cgcgtgccct gcggctggaa
      961 atagcgctcc tgcagctgcg gcgcgacgtc cttcaggtcg cgcgcggcgt aggcggcggc
     1021 gcgcgcggtg gcgagcgcct ggtcgtcgag gtcggtggcg tagatcttga cccgctcgag
     1081 gaacgcctcc acgcccagcg cctcggcgag cagcatggcg atgctgtagg cctcctcgcc
     1141 ggtggcgcag cccgccgacc agacgcgcac cggctcgccg ccggtcttcg cgcgcagcat
     1201 tgccggcagg acctcggcgg cgaggtactc ccaggcgggc gcgtcgcgga agaaggcggt
     1261 gacgttgatc aggatcgagt cgaacagctc ggcgaactcg cccggatgca cctcgaggtg
     1321 gtcgaggtac tcgtcgaagc ccggcacgcc gagcaggtcc atgcggtgct ggatgcggcg
     1381 cgtcaggctg ggccgcttgt agccgccgaa gtcgaagccg cggctggtct tcaggtaggc
     1441 gagcaggcgc tcgaagcgtg cctcgttgcc gttgccgccg ctcacgccgc ccgccgctcg
     1501 ccgagcaggg tgcgcagcgc gcccgagatc tcctcgagcg gcagcacgaa atccgccagg
     1561 ccggtctgcc gggctgcgcc cggcatgccc gagaattccg ccgtcgccgg atcctgcacg
     1621 atcagcgtgc cgcccatctt cttgatcgct ttcgcgccca tcgctccatc gcttcccgag
     1681 cccgaaagta tcacgccgat ggcgcgctcg cggtagctgg cggcgaccga ttcgagcagc
     1741 aggtcggccg aggggcgcga gaagtgcacc agctcggtcc gcgtgagcga gagcaggccg
     1801 tccggattga ccagcaggtg gcggtccggc ggcgcgatga acacgctgcc gggctgcaca
     1861 tgctcatcgt cggcggcctg ccgcacggcg agcggggtgc gccggccgag gatctcggcc
     1921 atcaggctgc ggtggcgcgg gtcgaggtgc tgcaccacca ccagcgccgc agggaaattg
     1981 gcgggcaggc ccgccagcac ctcgcttagc gccctgaggc cgccggccga ggctgccagc
     2041 gcgacgatct cgaagccgtc gggcatgccg gccgggccgg gctagtcgcc gcgccgcacc
     2101 agccagcaca gcgcggcgag gccgccgttg ggcaccggca tcgcgcgcac gcgaaagtgc
     2161 gccgcaaccg gctggccctg cgccggcgtg agcgcgccgc gccaggcgct tacctcgccg
     2221 tccggctggg cgctggcgcg caccacatgc gcgcggaagt cgcgtcgctc cgcctcggga
     2281 acgtagctca ccagcggctt gccgtcgagc gcgcgcgccg cgatgccgag cagctccgcg
     2341 gccgcgcggt ttgcctcgcg gatcgtgccg tgcgagtcgg tcaccaggta ggcgtcgggc
     2401 gcgtactcga agaactcggt gtagcgctgc cgctcgctcg ccagctcgtc ggagcgctcg
     2461 cgcaactcat gccacagcgc ctcgatttcc tgcagaccgg cctccagctc gccttgcgcg
     2521 tgcaacgtgc cgtcctcgag cttgcgctgc agcagctcgc gcaccacctt gaccgaggag
     2581 aacagggaga acgagtcgac ttgtgtcatc tactcgatat cccgcaaaaa ccagagcagg
     2641 ggaaggaaat tttccgcgtc tggcggcatc ggccgcaaat cgatcttcac ttcgcgcggc
     2701 tgcccgtcgg gcgacttgat gcgcgacagc cagccctcga tcgggtgggg atcgcgcacc
     2761 gccatcgcca ggtgcatgcg aaacgcctcg cgctcctcgt cggcgatgaa gatcgccagc
     2821 ggcttgccga aaaggtaccc ggcgggcaca tcgagcagcg cgacggcagc gaggttcgcc
     2881 tcgtgcacgt tgccgtggat gtcggtgatc aggcaggcga acggcgcgcg gtcgaaaagg
     2941 gctgcattgc gctcgcgctc gctggcgagc cggtcggcgt ggcggcccag ctcgtcccac
     3001 agcgcctcga tctcctcgac ggcgatctcc agctcgcgcg cgctcggccg gccttcgacg
     3061 tcggcgtgcg cgacggcgag ccttcccttg agacgcttca ggtcggtccc ggccatgcga
     3121 acaggatacg ctcccgggga gggttgagaa gcttttgcac tgtaggtctt tggatgacac
     3181 gcgggcggcc gccgtccgga ctaggggccg cggcaagccc ctcaacgtcg ctcttgcatc
     3241 ctgccgagga tgaaaatcag cgtgctgggg ttgggatacg taggagcggt atccgcgggc
     3301 tgccttgccc gcgacggcca cgaggtcatc ggggtcgatc cggagcgggt caaggtagac
     3361 ctgatcaatg aggggcgctc gccgatcatc gagaaggaca tcggcggcat catcgcagag
     3421 gcggtggcgg ccggccgtct ggccgcctcc accgatcttg aggccgcggt gcgccatacc
     3481 gacctcgcgc tggtgtgcgt cggcacgccg agcatgccga acggcggcct cgacctgcgc
     3541 tacgtcaggc gggtgtgcga gcagatcggc aagacgctcg ccgcgcacca cggcgcgccg
     3601 gtgatcgtga tgcgctcgac catgctgccc ggctcgatgc gcgaagtggt gatcccgacg
     3661 ctggagaaat tctccggcaa gcgggccggc gaggagttcg gcgtgtgcat caaccccgag
     3721 ttcctgcgcg aaggcacggc ggtgcacgac tactaccacc cgccgaagac cgtgatcggc
     3781 gaggtgaacc gcgcgagcgg cgacctgctc gccaacctct acggcaagat gccggggccg
     3841 atgatccgca ccgacatcga gaccgccgag atggtgaagt acgccgacaa cccgtggcac
     3901 gcgctgaagg tcggcttcgc caacgagatc ggcacgctgt gcaaggcgct gcaggtcgac
     3961 gcgcaccgcg tgatggaaat tttctgccag gacctgaagc tcaacctgtc gccctactac
     4021 ctgaagcccg gcttcgcctt cggcggctcg tgcctgccga aggacgtgcg cgcgctgctg
     4081 cacaaggcga agacgctcga cctcaacctg ccgatcctcg cctcgatcct gccctccaac
     4141 cagctgcaga tcgagcgcgg catccaggcg gtggtggaca agaccaaccg caaggtcggc
     4201 atcctcggct tctcgttcaa ggccggcacc gacgacctgc gcgagagccc gatggtcgag
     4261 ctgaccgagc gcctcatcgg caagggctac gacctgcgcg tctacgaccg caacgtcagc
     4321 ctggccgcgc tgcacggcgc gaatcgcgac tacatcctca accgcatccc gcatatctcg
     4381 cgcctgatgg tgccgacgat cgacgaggtg ctggcgcacg ccgagaccat cgtcatcggc
     4441 aacgccgcgc ccgagttcgc cgaggtgccc aagcgcatcg gcgacggcca gacggtgatc
     4501 gacttcgtgc gggtgtgcga ctcgcgcacc gtgctcggcg tctacgaagg gctgtgctgg
     4561 taaacgggat gagagtcggc aggaggtaaa tgcaatgcgc actttctttg tacgtcccga
     4621 gcgcgacggc ggctacggcg ccagcgacgg caccacttac gacaacgcct ggaacggcct
     4681 ggagaacgtc gactggcagg cgatggcggc gggcgatccg gcgcagctct ggctgtgcgg
     4741 cggccccggc gggccgggcg ggttcatgac cgtgttcgtc gaatggagct acttcgaaac
     4801 cgcgccgcag gcgctcagga ggcaggcatg gtcggctacc gtcggatgat cctcaacgcc
     4861 gtcgcgctgg ttcccggcgc ggcgcagttg ccgccggtcc gctcccggct gcagaagcgc
     4921 ggcgtcggca ccggcggcac gaacgacgcg cgctactgct acaccgtatg gctgcgccat
     4981 ctggtcacgg cggcgcgcct cggcctgaac cccgatccgc gcgagatcgc cgagctcggt
     5041 cccggcgact cgatcggcgc gggtctcgcc gcgctgctgt gcggcgccga gcgctacagc
     5101 gcgctcgacc tggtgagtca cgccaacccc gtgtccaacg tcctcatctt cgaccagctg
     5161 gtggagctgt tccgcgcgcg cgccgacctc cccggcgacg gcgactaccc gaacatctcg
     5221 ccgcggctcg acgattaccg cttcccggcg cagatcctgg acgaagcgcg catgcagcgc
     5281 gcgctgcacc cggagcgcgt cgcgcgcctg cgcgatgcgg tgctcggcga cgacccgtcg
     5341 ggcctcatcc gctaccgcgt gccctggcag gaggagagcg tgctcgagcg cggctcgcag
     5401 gacctggtct tctcgcaggc ggtgctcgag cacatcgacg cgctggccga ggcgtaccgc
     5461 gcgatgcgcc tgtggctgaa gccctcgggc cacgtctcgc accagatcga cttcaagtgc
     5521 cacgatctgg cggacacctg ggacggccac tggcgctacg gcgacctgcg ctggacgatg
     5581 ctgcgcggcc gggattcgtg gtccatcaac cggcagccgt attccgagca cctgcggctg
     5641 ctggagcaga ccggcttcga ggttcttgcg gcgcaccccg tgcagcgcga gccgtcgtat
     5701 ccgcgcagct cgctggcgcg gcgcttcagc gccatgacgg aggcggaccg cggcgtcagc
     5761 ggcgtgtacc tggtggcgcg accggtcctc tgaggcggct gaccgtgcgg cagcggcgtg
     5821 ctctgttcct ggtagagaat ctgcccagcc cgttcgaccg gcgggtgtgg caggaggcga
     5881 cggcgctcag ggacgccggt tacctcgtgt ccatcatctg cccgaccggc aaggggtacg
     5941 agaagacctt cgaggctctc gatgggatcg agatcttccg ctatcgcctg gcggaagcag
     6001 atggcgtagc gggatacctg ctcgaatatt ccgctgcgct tgccttcacg ttcgccctga
     6061 gctggaaagt attcttcagg caaggcttcg acgtcatcca tgcctgcaac ccgccggatc
     6121 tgttcttcct gatcggcggc ttctggaagc tgttcggcaa gaagttcctg ttcgaccacc
     6181 acgacgccag ccccgagctc tacgaagcca agttcggccg gcgcggcctg ctgtggaagg
     6241 cgatgatgct gctcgagaag ctcaccttca ggacggccga cgtctcgctc gcgaccaacg
     6301 agtcctacaa acgcattgcg gtgacccgcg gcggcatgcc gcccgaaaag gtctttgttg
     6361 tccgaagcgg cccttcgctc gagcggatga aaatcggttt tcacgatgag aagttgaaaa
     6421 agggcaagaa atacctcgtt ggctatgtag gagtgatggg cagacaagag ggtatcgact
     6481 atctgctgcg ggcggcggcg cacatcgtgc atgacctggg tcgcaaggac gtgcacttcg
     6541 gcctggtcgg cggcggtacc tcgctcgagg aaatgaagcg cctcgccgac gagctcggcc
     6601 tgaacgactg cgtgacattc accggccggg tgagcgacga gcagatgctg gcgatgctca
     6661 ataccgcgga tgtctgcgtg aatcccgacg ccgccaacga gatgaacgac atctcgacca
     6721 tgaacaaggt gatggagtac atggcgctcg gcaagccgat ggtgcagttc gatcttgccg
     6781 aggggcgttt ctcggcgcag cgggcctcgc tctacgcgcg gcgcaacaac gccttcgatc
     6841 tggcggcaaa aatcgtcgag ctcctggaca acccggagaa acgcgccgcc atgggcgaga
     6901 tcggccgcag gcgggtgctt gaagagctgg agtggcgcca cgaggtgccg aagctcctgc
     6961 aggcatacga ggcgctttgg gcgccggcaa agccgcgccc cgcttctccc gcggcgcgca
     7021 aaggtccagc gtggctaagc ggcggacgtg ggcgctgatc gcgctcgccg gcgcggcgct
     7081 cttcggggcg tattccgcgc agcgcagcgc tgacgagctg ctggttgcgg gcgccggcgc
     7141 ctccgcgccg tcgcgctcga gcgcaacgat tccggggaac ctgcccgagc gggacatcct
     7201 tcacgcgcag aaggcggatc tgttcgcacc gccatacgtc gctccgccgc cgccgccccc
     7261 accgccccca ccgccgctcg ctgcggctgc cgagcagccg tccaatccct atcgcttcgc
//
LOCUS       DASYJA010000267         2714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2309280, whole genome shotgun sequence.
ACCESSION   DASYJA010000267 DASYJA010000000
VERSION     DASYJA010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2309280"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VGO02_05745"
     CDS             <1..574
                     /locus_tag="VGO02_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV7477227.1"
                     /translation="DNTEQPAIFLRVAESQVGHGQPIIRPRESTHLDFEAEIAVIVGT
                     PGRRIPQADAWKHIAGYSCYNDGSVRDWQRHTVQWTAGKNFAQTGAFGPWMVSADEIP
                     PNTKMTLSCRLNGERMQHATTEQMIFRIPKIIEYVSAWTPLAPGDVLVTGTPGGVGAR
                     RNPPLWMKPGDKVEIEIDKVGILENTIADG"
     gene            579..929
                     /locus_tag="VGO02_05750"
     CDS             579..929
                     /locus_tag="VGO02_05750"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7477228.1"
                     /translation="MDKIKQTIRELESRRFRAMIEADAVALEALLADSMIYTHSSATT
                     DGKASYIAGVKSKKWQYRQIDRPIEDIQVHGDTVVVTGQVRIDILIDGKPKIMNSRYT
                     DVWVKGAKGWQMVA"
     gene            947..1870
                     /locus_tag="VGO02_05755"
     CDS             947..1870
                     /locus_tag="VGO02_05755"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEV7477229.1"
                     /translation="MRSFVLLFALYSGLVFSQEKPIRFYVGFAAGGSTDIISRLLGQK
                     LAERLKQPVVVEQRVGANDLVAKSAPDGASMVLLTGGRPGTAAVMKKLPYDPVKDFGM
                     VSVVIEYPMVISVAPESPIKSFPDLIARAKAAPGQVSFSSAGPGSLHHLLGEWMNIEA
                     GTNMLHVPFKGAAPAFTDLLGGRIDVLIETGTFSIPLVKSGRLRALALSSAGRYPLLP
                     EAPTVGETLKGVEFSSWLGLAVSPGTPRATIEHLNRELGAILAESEVKERFAGFGGVP
                     AHSSPEAMRERVEREIARWQKVVDTRGIERQ"
     gene            1881..>2714
                     /locus_tag="VGO02_05760"
     CDS             1881..>2714
                     /locus_tag="VGO02_05760"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7477230.1"
                     /translation="MNPAPVIDVHTHMLNKEWLSLLEQHGGPRYTLKEVKGGLKAIHL
                     DGSPFMTPVPNMFDWEARIANMSKARVDIAVTSLTCPNAFWGSPQISLKAARVMNEEM
                     AKAQQTWPDRIRWFASLPWQHQDLALSELKRSCENGAVGVMVLANIGGKALTDPSFEK
                     IWKEIDKRGLPVLVHPSAPPGSAELGLHEFQLTAPIGFTFDTSLAIARCIYDGFFDRF
                     PNLKLIASHGGGALPYLVGRLDICWDNIPAARAKTAEPPRNYMRRIYVDSVVFRQDVL
                     DM"
BASE COUNT          508 a          928 c          862 g          416 t
ORIGIN      
        1 cgacaacacc gagcagccgg cgatcttcct gcgcgtggcc gagtcgcagg tgggccacgg
       61 ccagccgatc atccggccgc gcgagtcgac gcacctcgac ttcgaggccg agatcgccgt
      121 gatcgtcggc accccgggaa ggagaatccc gcaggccgat gcctggaagc acatcgccgg
      181 ctatagctgc tacaacgacg gctcggtgcg cgactggcag cgccacaccg tccagtggac
      241 cgccggcaag aatttcgcgc agaccggcgc cttcggcccg tggatggtga gcgccgacga
      301 gatcccgccc aacacgaaga tgacgctgtc ctgccggctg aacggcgagc gcatgcagca
      361 cgccaccacc gagcagatga tcttcaggat cccgaagatc atcgagtacg tttcggcgtg
      421 gaccccgctc gctcccggcg acgtgctggt gaccggaacg ccgggcggcg tcggcgcccg
      481 gcgcaacccg ccgctctgga tgaagccggg cgacaaggtc gagatcgaga tcgacaaggt
      541 cggcatcctc gagaacacca tcgccgatgg ctgaccctat ggacaagatc aagcagacca
      601 tccgcgagct ggagtcgcgc cgcttccgcg cgatgatcga ggcggacgcg gtcgcgctgg
      661 aggcgctgct cgccgacagc atgatctaca cgcactcttc cgccacgacc gacggcaagg
      721 cgagctacat cgccggcgtg aagtcgaaga agtggcagta ccgccagatc gaccggccga
      781 tagaggacat ccaggtgcac ggcgacacgg tcgtcgtcac agggcaggtg cgcatcgaca
      841 tcctcatcga cggcaagccc aagatcatga acagccgcta caccgacgtc tgggtgaaag
      901 gcgcgaaggg ctggcagatg gtggcctgac aatcgacgcc cattccatga ggagttttgt
      961 acttctcttc gccctttact ccggacttgt tttttctcaa gaaaaaccca ttcgcttcta
     1021 cgtcggcttt gcggccggcg gcagcaccga catcatcagc cgcctgctcg gccagaagct
     1081 cgccgagcgc ctgaagcagc cggtggtggt cgagcagagg gtcggcgcca acgacctggt
     1141 ggcgaagtcg gcgcccgacg gggcctcgat ggtgctgctc accggcggcc gcccgggcac
     1201 cgcggcggtg atgaagaagc tgccctacga cccggtgaag gacttcggca tggtgagcgt
     1261 ggtcatcgaa tacccgatgg tcatctcggt ggcgccggag tcgccgatca agtcctttcc
     1321 cgacctgatc gcgcgcgcca aggccgcgcc gggccaggtg agcttctcct cggccgggcc
     1381 cggcagcctg caccacctgc tcggcgaatg gatgaacatc gaggccggaa ccaacatgct
     1441 gcacgtgccg ttcaagggcg cggcgccggc gttcaccgac ctgctcggcg ggcgcatcga
     1501 tgtgctgatc gagaccggaa ccttctccat tccgctggtc aagagcggcc ggctgcgcgc
     1561 gctcgctctg tcctcggccg ggcgctatcc cctcctgccc gaggcgccga cggtaggcga
     1621 aacgctgaag ggcgtcgagt tcagctcctg gctcggcctc gcagtctcgc ccggcacgcc
     1681 gcgcgccacc atcgagcacc tgaatcggga actgggggcg atcctcgcgg agagcgaggt
     1741 caaggagcgc ttcgccggct tcggcggcgt gcccgcgcat agcagccccg aggcgatgcg
     1801 tgagcgcgtc gagcgcgaga tcgcccgctg gcagaaggtc gtcgatacgc gcggcatcga
     1861 acgtcaatag gaagaaactc atgaatccag cgccggtgat cgacgtccac acgcatatgc
     1921 tcaacaagga gtggctctcc ctcctcgagc agcacggcgg cccgcgctac acgctgaagg
     1981 aagtgaaggg cggcctgaaa gcgatccacc tggacggctc gccgttcatg accccggtgc
     2041 cgaacatgtt cgactgggaa gcgcgcatcg ccaacatgag caaggcgcgc gtcgacatcg
     2101 ccgtcacctc gctcacctgc ccgaacgcct tctggggctc accgcagatc agcctcaagg
     2161 cggcgcgcgt catgaacgaa gagatggcta aggcgcagca gacctggccc gaccgcatcc
     2221 gctggttcgc ctccctgccc tggcagcatc aggatttggc tttatccgaa ttaaaaagaa
     2281 gttgcgagaa cggagccgtg ggcgtcatgg tcctcgccaa catcggcggc aaggccctga
     2341 ccgatccctc gttcgagaaa atctggaagg aaatcgacaa gcgcggcctg ccggtgctgg
     2401 tgcacccgag cgcgccgccg ggcagcgccg agctaggcct gcacgagttc cagctcaccg
     2461 cgcctatcgg cttcaccttc gacacctcgc tcgccatcgc gcgctgcatc tacgacggtt
     2521 tcttcgaccg cttcccgaac ctcaagctga tcgcctcgca cggcggcggc gccctgccct
     2581 acctcgtcgg ccgcctcgat atctgctggg acaacatccc ggccgcgcgc gccaagacag
     2641 ccgagccgcc gcgcaactac atgcgccgca tctacgtcga cagcgtggta ttccgtcagg
     2701 acgtgctcga catg
//
LOCUS       DASYJA010000268         3018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2582464, whole genome shotgun sequence.
ACCESSION   DASYJA010000268 DASYJA010000000
VERSION     DASYJA010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2582464"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..359)
                     /locus_tag="VGO02_05765"
     CDS             complement(129..359)
                     /locus_tag="VGO02_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HEV7477231.1"
                     /translation="MSKILLASIVLSAAAVAGCSGWGKGETVGTVGGAAAGGAIGSAA
                     TGGSTVGTVGGAVLGGAVGNQVGENYDDKHKK"
     gene            complement(396..572)
                     /locus_tag="VGO02_05770"
     CDS             complement(396..572)
                     /locus_tag="VGO02_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477232.1"
                     /translation="MSLAAPAFTDLFFGMEAFVQRIQQWRRQQEGKMGYILLWALGVP
                     IPVLFVIFLLRGCT"
     gene            639..1907
                     /gene="epsL"
                     /locus_tag="VGO02_05775"
     CDS             639..1907
                     /gene="epsL"
                     /locus_tag="VGO02_05775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtB/PEP-CTERM-associated polysaccharide
                     biosynthesis outer membrane protein EpsL"
                     /protein_id="HEV7477233.1"
                     /translation="MTFPAARLLVALALSAAAAAVAQPAEEGLPEGEESETDPEAVLE
                     PEMVKPPAVAALPDRPVMARVGGSLSYDSNLFRSPDARSERYATAYAGLSIDKAYAQQ
                     RIRLDITETAYRYDNFSYLDFDALNYLGAWDWQLGPRIGGTLSATRVQSLVDYSEFRR
                     PGELNVRTTENFALGADAWLVGGWHLTGALSRVQNHYSVPFPQEGSYQADGREAGVRW
                     VARSANWLAFNLRSLDGRYVDRPLDPVAQLDDGFRRTETEALAAWRLTGKSSFDGRAA
                     WIDYRSDNFSARDFSGPAGRLRYLWQTTPKLGLTVGLAREIEPWTAANASYRVDQRLS
                     LGAAWQAAARATLRLEARRGESDFRAPLPGFTGTPRHDKEGSVQFNAEWRALRNLILN
                     AGAQRYRQSSNDPAANFHGGQVTAGASFLF"
     gene            complement(1911..2906)
                     /locus_tag="VGO02_05780"
     CDS             complement(1911..2906)
                     /locus_tag="VGO02_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477234.1"
                     /translation="MPASITVAAGAASASFNAVTSPVATPITVTISGAYGGATQTGSL
                     TVVPPRVAALSLSTSSVTGGNPVTGTVTLDGPAPAGGAQVALSDNSGATSVPATVTIA
                     AGVASATFNIATSPVAVSTSASISASYGGGTQSVSLTVLPPAVSALNVSPASVRGGTS
                     ATATVMLTAPAPAGGAPVTLSDNGAAASVPATVTIPAGATSASFSVGTSAVASNTAVT
                     ISASYGGATRTATLTVLPAVLTSLTLNPTSVVGLLGSSTGTLTLNGPAPAGGAVVSVS
                     DNSGACSAPSTVTIPAGASSATFTVRTGLVLVSTTCTVTGTYQGASRSASLQVTL"
BASE COUNT          483 a         1051 c         1028 g          456 t
ORIGIN      
        1 gccaaatcca cccgcgccgc gcgctccgcg gcggcagaaa ccgcaatctt ctcgactcgc
       61 cccatgcggc aattataggt acaaacaaca agggcgcccg aaggcgccct tgtttgccgc
      121 tgcaaccgct acttcttatg cttgtcgtcg tagttctcgc cgacctggtt cccgactgcg
      181 ccgccgagca cggcgccgcc caccgtcccg accgtgctgc cgccggtcgc cgcagagccg
      241 atcgcgccgc cagctgccgc gccgcccacc gtgccgacgg tctcgccttt gccccagccg
      301 ctgcagccgg ctacggccgc cgcggacagg acgatggatg ccaataggat cttgctcata
      361 tcattgcctc ctgttttaac aacggtgaaa ggggactagg tgcagcctcg cagcaggaaa
      421 atcacgaaga gcaccgggat aggcacgcca agcgcccaga ggaggatgta gcccatcttt
      481 ccctcctgct gcctgcgcca ctgctgaatg cgctgcacaa aagcctccat gccgaaaaag
      541 agatcagtga aggcaggagc agcaagcgac atgcccaacg cgactttttg actcgtaggt
      601 aaaatcccta cgctgaagtg cgaaaaaaag gatcaggcat gacgttcccg gcggctcgcc
      661 ttcttgtcgc gctcgcgctg agcgccgcgg ccgcggccgt ggcgcagccg gcggaagaag
      721 ggctgcccga gggggaagag tcggagaccg atcctgaggc ggtactcgag cccgaaatgg
      781 tgaagccgcc ggccgtcgcg gcgctgcccg accggccggt gatggcgcgc gtcggcggct
      841 cgctcagcta cgacagcaat ctcttccgct cgcccgatgc gcgctcggag cgctacgcca
      901 ccgcgtacgc gggcctgagc atcgacaagg cttacgcgca gcagcgcatc cggctcgaca
      961 tcaccgagac cgcgtaccgc tacgacaatt tctcctacct cgacttcgac gcgctgaatt
     1021 acctcggcgc ctgggactgg cagctcggcc cgcgcatcgg cggaacgctg agcgcgacgc
     1081 gcgtgcaatc gctcgtcgac tacagcgagt tccgccgtcc cggcgagctc aacgtgcgca
     1141 ccaccgagaa tttcgcgctc ggcgcggacg cgtggctcgt cggcggctgg cacctcaccg
     1201 gcgccctgag ccgcgtgcag aaccactact cggttccctt cccccaggaa ggcagttacc
     1261 aggcggacgg gcgcgaggcc ggcgtgcgct gggtcgcgcg gtcggcgaac tggctcgctt
     1321 tcaacctgcg ctcgctcgac ggccgctacg tcgaccgccc gctcgacccg gtggcgcagc
     1381 tcgacgacgg tttccggcgc accgaaaccg aggcgctcgc cgcgtggcgc ctcaccggca
     1441 agtcgagctt cgacggccgc gcggcgtgga tcgactaccg ctcggacaat ttctccgcgc
     1501 gcgatttttc cggcccggcg ggaaggctgc gctatctctg gcagacgacc cccaagctcg
     1561 gcctgactgt cggcttggca agagaaatcg aaccctggac ggctgcgaac gcgagctacc
     1621 gtgtagacca gagactgtcg ctcggggcgg cctggcaggc ggccgcgcgc gctacgctgc
     1681 gcctcgaggc aaggcggggg gaaagcgatt tccgcgctcc gttgccgggc ttcaccggca
     1741 cgccgcgcca cgacaaggaa ggcagcgtcc agttcaacgc ggagtggcgc gcgctgcgca
     1801 atctgatact gaacgccggg gcgcagcgct accggcagtc ctcgaacgat cccgcagcga
     1861 atttccacgg cggccaggtc accgccggcg cgtcgtttct gttctagctt ctacagggta
     1921 acttgcaggc tggcgctgcg gctcgcgccc tggtacgtac ccgtgaccgt gcacgtagtc
     1981 gagaccagga cgaggcccgt cctcacggtg aaggtcgcgc tgctggcgcc ggccgggatg
     2041 gtcacggtgc ttggcgccga gcacgccccg ctgttatcgg agaccgagac gaccgcgccg
     2101 ccggcggggg ccggcccgtt cagcgtcagc gtgccggtcg aacttccgag caggccgacg
     2161 acgctcgtcg ggttgagcgt gagcgaggtg agaaccgcgg gcagcacggt gagggttgcc
     2221 gtccgcgtcg cgccgccgta agaggcggag atcgtgaccg ccgtgttgct ggccaccgcg
     2281 ctggtgccca cgctgaagct cgcgctcgtg gctccggccg ggatggttac agtggcaggc
     2341 acggaggctg ccgcgccgtt gtcggaaagt gtgactggcg cgccgcccgc cggcgcaggc
     2401 gcagtcagca tcaccgtagc cgtggcagaa gttccgcccc taacgctcgc cgggctcacg
     2461 ttcagcgccg agacggccgg cggcagcacg gtgagcgaca cgctctgcgt gccaccgccg
     2521 taggaggcgg agatgctcgc ggaggtcgac acggcgaccg ggctggtggc gatgttgaac
     2581 gtcgcgctgg cgactccggc cgcgatggtc acggtggcag gcaccgaggt tgccccgctg
     2641 ttgtcggaaa gcgcgacctg cgcgccgccg gcgggcgccg gcccgtcgag cgtcaccgtg
     2701 cccgtaacag gattgccgcc cgtgacgctc gatgtgctca ggctgagcgc tgcgacgcgc
     2761 ggcggcacca cggtgagcga gccggtttgc gtggcgccgc cgtaggcgcc ggagatcgtg
     2821 actgtgatcg gagtcgcgac cgggctcgtc accgcgttga agctcgcgct ggcggctccg
     2881 gccgcaaccg ttatgctcgc cggcactgtg accgcggcgt tattgctgga gagcgcgacc
     2941 tgcgcgccgc ccgccggcgc aggcgcagtc agcgtcaccg tagccgtggc agaagttccg
     3001 cccctaacgc tcgccggg
//
LOCUS       DASYJA010000269         1678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2582949, whole genome shotgun sequence.
ACCESSION   DASYJA010000269 DASYJA010000000
VERSION     DASYJA010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2582949"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..614)
                     /locus_tag="VGO02_05785"
     CDS             complement(234..614)
                     /locus_tag="VGO02_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477235.1"
                     /translation="MLRILLSTALLAFCAPVFAQAPSDAPRPKADRRFDCSKAKDPKG
                     CEERVAKAKAAHEKARKACEGKSGEEGRDCMRHEMCAQAQDPAKCEAQVKEAAARRAK
                     IREACKDKTGEELKSCVREQRQKK"
     gene            complement(712..1221)
                     /locus_tag="VGO02_05790"
     CDS             complement(712..1221)
                     /locus_tag="VGO02_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEV7477236.1"
                     /translation="MAIKVGDRLPDGKLMESTEFDSGTQCAMPPKEVSVADAVRGKKI
                     AIFGLPGAYTPTCSAKHVPGYAQNAEALKKKGVHEIWCVATNDAFVMAHWGRDQKALG
                     KIRFLADGSAAWTKALGLELDLNARGMGTRMNRFSMLVDNGVVKQLNVEAPGKFEVSD
                     AATMLKQAG"
     gene            1331..>1678
                     /locus_tag="VGO02_05795"
     CDS             1331..>1678
                     /locus_tag="VGO02_05795"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEV7477237.1"
                     /translation="MDHRRVTWQVRERLTWLAWLLDSSIPIPGTRLTIGLDALIGLFP
                     VVGDAVGVVLSSYIVREAAALGAPRSILARMAFNVALEGLIGMIPLAGDVFDAAYKAN
                     QRNVRLLTAWLDAP"
BASE COUNT          240 a          585 c          545 g          308 t
ORIGIN      
        1 cgccttcgat cgaagttgct ttttggaggc gtgcaggcga cgccttctgc accggcggcg
       61 gcggcgcagc agtctgctgc gccagggcgc cggtggcgaa gagcagcagg aaggcgacgg
      121 cgtaaagccg gtttgtcata gcagccagtc gaataggtgc agcgccggga gacctcaccc
      181 gatcgtagcg aagctgaggc gccagcgcca tgctaccgcg accgccacca gccctatttc
      241 ttctggcgct gctcgcggac gcagctcttc agctcttcgc cggtcttgtc cttgcacgcc
      301 tcgcggatct tggcgcgccg cgcggcggct tccttcacct gcgcctcgca cttggccggg
      361 tcctgggcct gggcgcacat ctcgtggcgc atgcaatcgc gcccctcctc gccgctcttg
      421 ccttcgcagg ccttgcgcgc cttttcatgc gcggccttcg ccttggcaac gcgctcctcg
      481 cagcccttgg ggtccttcgc cttggagcag tcgaaacgcc gatctgcctt cggcctgggc
      541 gcatccgaag gcgcctgggc gaaaaccggg gcacagaacg ccagcagggc ggttgaaagc
      601 aggatcctga gcatgcgatg tcctttcggt cggtgggagg cccgcgcagg gcctcgccct
      661 acaacgcgcc gacccggcgc cgcgctgaca gcaaaatgcg aaagtcctgc ttcagcccgc
      721 ctgcttcaac atcgtggccg cgtcggatac ctcgaacttg cccggagcct cgacgttcag
      781 ttgcttcacc acgccgttgt cgaccagcat cgagaaccgg ttcatgcgcg tgcccatgcc
      841 gcgcgcgttg aggtcgagct ccaggccgag cgctttggtc caggcggcgc tgccgtcggc
      901 cagaaacctg atcttgccga gcgccttctg gtcgcggccc cagtgcgcca tgacgaaggc
      961 gtcgttggtg gcgacgcacc agatctcgtg cacccctttc ttcttcagcg cctcggcgtt
     1021 ctgcgcgtag cccggcacgt gcttcgccga gcaggtgggg gtgtacgcgc ccggcaggcc
     1081 gaagatcgcg atcttcttgc cgcgcacggc gtcggcgacg ctcacttctt tgggcggcat
     1141 ggcgcactgc gtgcccgaat cgaactccgt ggattccatc agcttgccgt cgggaagccg
     1201 gtcgccgacc ttgatcgcca tagcaggtct cctgtggatc gtctgggggg aaggcggaat
     1261 gatacccaac tcgtaacatg cgcggagcac gcgcggaacg ttccgggccg cgcgcggccc
     1321 aaccacgggc atggaccacc gtcgcgtcac ctggcaggtt cgcgagcggc tcacctggct
     1381 cgcgtggctg ctcgacagct cgatcccgat ccccgggacc cgccttacga tcgggctgga
     1441 tgcgctgatc gggcttttcc cggtcgtcgg cgacgccgtc ggcgtcgtcc tttcgagcta
     1501 tatcgtgcgc gaggcggccg cgctcggcgc tccgagaagc atcctcgcgc gcatggcgtt
     1561 taacgtcgcg ctggaaggat tgatcggcat gattccgctc gccggcgacg tgttcgacgc
     1621 ggcctacaag gccaaccagc gcaacgtgcg cctgctcacc gcctggctcg acgcgccg
//
LOCUS       DASYJA010000270         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_312320, whole genome shotgun sequence.
ACCESSION   DASYJA010000270 DASYJA010000000
VERSION     DASYJA010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312320"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /gene="boxB"
                     /locus_tag="VGO02_05800"
     CDS             complement(<1..902)
                     /gene="boxB"
                     /locus_tag="VGO02_05800"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEV7477238.1"
                     /translation="MGAEDTVSDAAGAISADRFVEISYDEVIPNNVALGSDKQLQRAL
                     EGWRPKYLDWWTGMGPVGFQQSEVYLRTAVGVDPAGWAKFGYVKMPTYRWGILLAPKV
                     EGRTIPFGRHKGEPAWQEVPGEHRALLRRLIVVQGDTEPASVEQQRLLGATAPSLYDM
                     RNLFQVNVEEGRHLWAMVYLLVKYFGKDGREEAQALLERQSGQINNPRILGAFNEQTP
                     DWLSFFMFTFFTDRDGKMQLAALAESGFDPLSRTCRFMLTEEAHHMFVGETGVQRTIE
                     ATCAAMVKAGVKDPNEVEAVRKLGV"
     gene            complement(930..>2168)
                     /gene="boxA"
                     /locus_tag="VGO02_05805"
     CDS             complement(930..>2168)
                     /gene="boxA"
                     /locus_tag="VGO02_05805"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxA"
                     /protein_id="HEV7477239.1"
                     /translation="LIDPEICIRCNTCESTCPVGAITHDARNYVVDANICNLCMACVP
                     PCPTGSIDNWRAMPHSRAYSVEAQFEWDSLPAELSADELGGAGASAAALGEPTARSAP
                     SEAGGEAAFEVGAYAAVIPPWSAASPYANLYGPKAATRSVTGTVAGNVRVTEAGKDYD
                     THHIVLDFGALPFPVLEGQSIGIIPPSVGTDGGPHHPRQYSVASPRNGERPGYNNVSL
                     TVKRVLEDHQGRPARGIVSNYLCNLNVGDKVEVTGPFGVSFLMPNHPKSAIVMICTGT
                     GSAPMRAMTEWRRRLRKSGKFEGGKLMLFFGARTKEELPYFGPLGNLPKDFIDINFAF
                     SRTPGQPKRYVQDVMRERAADLAPLIADPHAYFYVCGLKAMEEGVVLALRDIAINAGL
                     DWETVGPALKQHGRLHLETY"
BASE COUNT          364 a          693 c          701 g          410 t
ORIGIN      
        1 acgccgagct tgcgcaccgc ctcgacctca ttgggatcct tgacgccggc tttgaccatc
       61 gccgcgcagg tcgcctcgat cgtgcgctgg acgccggttt cgccgacgaa catatggtgc
      121 gcctcttcgg tcagcatgaa gcggcacgtg cgcgagagcg gatcgaagcc gctctcggcg
      181 agcgccgcga gttgcatctt gccgtcgcgg tcggtgaaga aggtgaacat gaaaaacgac
      241 agccagtctg gcgtctgctc gttgaacgcg ccgaggatgc gcgggttgtt tatctgcccg
      301 ctctggcgct cgagcagcgc ctgcgcctcc tcgcggccat ccttgccgaa atattttacg
      361 agcaggtaga ccatcgccca caggtggcgg ccttcctcga cattgacctg aaacaggttg
      421 cgcatgtcgt agaggctcgg cgccgttgcg cccaacaagc gctgctgctc gaccgaagcg
      481 ggctccgtgt cgccctgcac gacgatgagc cggcgcagaa gcgcgcggtg ctcgccgggc
      541 acttcctgcc atgctggctc gcccttgtgg cggccgaatg gaatcgtacg cccctcgacc
      601 ttgggcgcga gcagaatgcc ccagcgatag gtcggcatct tcacatagcc aaacttcgcc
      661 cagccggccg gatcgacccc gacggcggtg cgcagataga cctccgactg ctggaatccg
      721 actggcccca tgccagtcca ccagtcgaga tatttcggcc gccagccttc gagcgcgcgc
      781 tgaagctgct tgtccgagcc gagcgcgaca ttgttgggaa tgacctcgtc gtagctgatc
      841 tcgacgaagc ggtcggcgga gatggcgcct gcggcgtcgc tcacggtgtc ttcggcgccc
      901 atgggtctct cctgttcgaa ggttgcaagc tagtaggtct cgagatgcag tcgcccgtgc
      961 tgcttgagcg caggcccgac ggtctcccag tcgagcccgg cgttgatcgc gatgtcgcgc
     1021 aatgcgagca ccacgccttc ctccatcgcc ttgaggccgc agacgtagaa ataggcgtgc
     1081 ggatcggcga tgagcggcgc cagatcagcc gccctctcac gcatcacgtc ctgcacgtag
     1141 cgtttcggtt gaccgggcgt gcggctgaaa gcgaagttga tgtcgataaa gtccttcggc
     1201 agattgccga gaggcccgaa atacggcagt tcctctttgg tgcgcgcgcc gaagaacagc
     1261 atcagcttgc cgccctcgaa tttgcctgac ttgcgtaggc gtcgccgcca ctcggtcatc
     1321 gcgcgcatcg gcgcgcttcc cgtgccggtg cagatcatca cgatcgcgct tttcggatgg
     1381 ttcggcatga ggaagctcac gccgaacggc ccggtcacct cgactttgtc gccgacgttg
     1441 aggttgcaga gataattgct gacgattccg cgcgctgggc ggccctggtg atcctccagc
     1501 acccgcttca cggtgagtga tacgttgttg tagccggggc gctcgccatt gcgagggctt
     1561 gcgaccgaat attgtcgcgg atgatgcggc ccgccgtccg taccaacgct aggaggaatg
     1621 attccgatcg actgcccttc gagcaccggg aacggcagcg cgccgaaatc gagcacgatg
     1681 tgatgggtgt cgtagtcctt gcctgcttcg gtgacgcgaa cattgccggc gacggtgccg
     1741 gtgaccgaac gcgtcgcggc cttcggacca tagaggttgg cgtaaggact ggcggcagac
     1801 cagggcggga tcacggctgc ataggcgccg acctcgaacg ccgcttcccc gcctgcttcg
     1861 ctgggcgcgg atcgggccgt cggttctccc agcgccgcgg cggaagcgcc ggccccgccc
     1921 agttcatcgg cgctcagttc cgcgggcaag gaatcccatt cgaactgcgc ctcgacgcta
     1981 taggctcgcg aatgcggcat tgcgcgccag ttgtcgatgc tgccggtcgg gcacggcggt
     2041 acacaggcca tgcagaggtt gcaaatattc gcgtcgacca catagttgcg cgcatcatgc
     2101 gtgatcgcgc cgaccgggca ggtcgactcg caggtgttgc aacggatgca gatttcgggg
     2161 tcgatcag
//
LOCUS       DASYJA010000271         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1947573, whole genome shotgun sequence.
ACCESSION   DASYJA010000271 DASYJA010000000
VERSION     DASYJA010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1947573"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VGO02_05810"
     CDS             complement(<1..300)
                     /locus_tag="VGO02_05810"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEV7477240.1"
                     /translation="MAVAQKAPLPARLAGLVREAKWLLLGALAFYLLLVFVTYHRGDP
                     GWSHTGSDAVARNAGGALGAWLADIFLYLFGLSAYWLVVLCACAVLWGYRRLDGRS"
     gene            complement(302..970)
                     /locus_tag="VGO02_05815"
     CDS             complement(302..970)
                     /locus_tag="VGO02_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV7477241.1"
                     /translation="MTTTVSTAVLKAVPLFSSFPEDALRTLTSMVARKSVSRSTTVMT
                     GGDPTDSLYIVLSGRLKVMMSDAEGKEVILTILGPGEFFGEMGLIDDEPRSATVVAIE
                     PCELLYIAKRDFKKCLAENFDMAMAVMRGLVRRLREADRKIGSLALLDVYGRVARLLL
                     DMSENVDGEKIVTKRLPKQDIAKMIGASREMVSRVMKDLQMGGYIEMRGSNIVLRDTI
                     MLPE"
     gene            1112..>1611
                     /locus_tag="VGO02_05820"
     CDS             1112..>1611
                     /locus_tag="VGO02_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7477242.1"
                     /translation="MPTRHARLLILGSGPAGYTAAVYAARANLRPVLLTGIAQGGQLM
                     TTTDVDNWPADADGVQGPDLMQRFQKHAERFETEVINDHIHKVHLERRPFLLEGDAGQ
                     YTCDALVIATGATAKYLGLPSEQKFMGRGVSACATCDGFFYKGEDVVVVGGGNTAVEE
                     ALYLAN"
BASE COUNT          282 a          552 c          514 g          263 t
ORIGIN      
        1 cgagcggccg tccaggcgcc ggtagcccca cagcaccgcg caggcgcaga gcacgaccag
       61 ccagtacgcc gaaaggccga acaggtagag gaagatgtcg gcgagccagg cgcccagcgc
      121 gccaccggcg ttgcgcgcca cggcatcgct tccggtgtgc gaccagccgg gatcgccgcg
      181 atggtaagtg acgaaaacca gcaggaggta gaacgccagc gcaccgagga ggagccactt
      241 cgcttcgcgg accagccccg cgagtcgcgc cggaagcggg gctttctgcg ctacggccaa
      301 gttactccgg cagcatgatg gtgtcgcgca gcacgatgtt cgagccgcgc atctcgatgt
      361 agccgcccat ctgcaggtcc ttcatgaccc ggctcaccat ttccctggag gcgccgatca
      421 tcttggcgat gtcctgcttg ggcaggcgct tggtgacgat cttttcgccg tcgacgttct
      481 ccgacatgtc gagcagcagc cgcgccacgc ggccgtagac gtcgagcagc gcgaggctgc
      541 cgatcttgcg gtccgcctcg cgcaggcgcc gcaccaggcc gcgcatcacc gccatcgcca
      601 tgtcgaagtt ctccgccagg cacttcttga agtcgcgctt ggcgatgtag aggagttcgc
      661 agggctcgat cgccaccacg gtggccgagc gcggctcgtc gtcgatgagg cccatctcgc
      721 cgaagaactc gcccgggccg agaatggtga ggatcacttc cttgccctcg gcgtcgctca
      781 tcatcacctt gagccggccc gaaagcacga tgtagagcga gtcggtcgga tcgccgccgg
      841 tcatcacagt ggtcgagcgc gacacgctct tgcgggccac catgctggtc agggtgcgga
      901 gcgcgtcttc ggggaagctg gagaacagcg ggactgcctt caggaccgct gtggagactg
      961 ttgttgtcat ggccacgttt tttccctccc tttgcgcgat tatggtagca gaactgcctc
     1021 ggcgcttccg ggctgttgcg ccgccgcctc acctataatc cgccactcat ccataaattc
     1081 ccgccactca tccggaagtg acgtttcgcg aatgcctacg cgccacgccc gcctgctgat
     1141 cctcggctca ggcccggccg gctacaccgc cgcagtgtac gcagcgcgcg ccaatctgcg
     1201 cccggtcctg ctcaccggca tcgcgcaagg cggccagctc atgacgacca ccgacgtcga
     1261 caactggccg gcggacgcgg acggcgtgca gggccccgac ctgatgcagc gcttccagaa
     1321 gcacgccgag cgcttcgaaa ccgaagtcat caacgaccac atccacaagg ttcacctcga
     1381 gcgccggccg ttcctgctcg agggcgacgc cggccagtac acctgcgatg cgctggtgat
     1441 cgccaccggc gccacggcga aatacctggg gctgccctcc gagcagaagt tcatgggacg
     1501 cggggtctcg gcctgcgcga cctgcgacgg cttcttctac aagggagaag acgtggtggt
     1561 ggtcggcggc ggtaacaccg cggtggagga agcgctctac ctcgcgaaca t
//
LOCUS       DASYJA010000272         3404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_676857, whole genome shotgun sequence.
ACCESSION   DASYJA010000272 DASYJA010000000
VERSION     DASYJA010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676857"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1009)
                     /locus_tag="VGO02_05825"
     CDS             complement(47..1009)
                     /locus_tag="VGO02_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7477243.1"
                     /translation="MARALAFLCAVILASGSAFGQGFPTKPVRFVVGFTPGGPSDILA
                     RALGQKLAKRWSQQVVVDNRPGAGGNLAAEAAAKSAPDGHTWLLGNNSILATNQSLYR
                     SLPYDPVKDFAPVALVAIQPNILVVHPSLPVSSVEELIQFSKANPGKLNYASSGSGAA
                     AHLAGELFKAMAGVDLVHVPYKGAQPALTDVIAGQVQMMFATSASVIPYIKAGRLRAL
                     AVTTAQRSASVPELPTVAEAGVPGFEATTWHGVVVPAGTPLPTVQFLNEAINAVLKEK
                     DLKDRLNSLGAEIATGTPKEFADYIAREIPKWAKVVKDSGAKPE"
     gene            complement(1042..1467)
                     /gene="dksA"
                     /locus_tag="VGO02_05830"
     CDS             complement(1042..1467)
                     /gene="dksA"
                     /locus_tag="VGO02_05830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEV7477244.1"
                     /translation="MAVELTRKGFTPYAAKKGEEYMNGAQRKHFRKILETLKSELSEE
                     IDRTVHTMQDEATVFADPNDRASQESDMALELRNRDRERKLIKKIDETIARIDADEYG
                     YCDSCGVEIGLKRLEARPTATLCIDCKTLDELRERQVAK"
     gene            1580..1656
                     /locus_tag="VGO02_05835"
     tRNA            1580..1656
                     /locus_tag="VGO02_05835"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1614..1616,aa:Pro,seq:tgg)
     gene            1673..1749
                     /locus_tag="VGO02_05840"
     tRNA            1673..1749
                     /locus_tag="VGO02_05840"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1707..1709,aa:Arg,seq:tct)
     gene            1796..1872
                     /locus_tag="VGO02_05845"
     tRNA            1796..1872
                     /locus_tag="VGO02_05845"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1830..1832,aa:His,seq:gtg)
     gene            1902..2735
                     /locus_tag="VGO02_05850"
     CDS             1902..2735
                     /locus_tag="VGO02_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477245.1"
                     /translation="MRFAVATPDPAAWLHVRAFDEVAEALCEGLRDLGHACERLTGSL
                     SSDGTNILFGAHHLSRAFVPPPGTILFNLEQIVPGSLWVSPDYLALLRQYRVWDYSAR
                     NVEALRAAGVARVSHAPLGYVASLERIVAQADDIDVLFYGRLIPRRVELLDRMSAYGM
                     RVVAVNDAYAEARDALIASAKVILNVHVAEEARLEAARCFYAMINGRFVLSEDSPDAI
                     ESGLAGGMAFAAYGDLLERCAWYLARPAERAAVAHAGRSLMRARPQAALLRPALAELE
                     T"
     gene            2774..>3404
                     /locus_tag="VGO02_05855"
     CDS             2774..>3404
                     /locus_tag="VGO02_05855"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEV7477246.1"
                     /translation="MREGTSASAAAGAILQAALAQARSNARGAIDSPHAEHLHQLRVG
                     LRRYRSALRLFRDLLRKKPRRRLARRSRKAMEPLGPVRDWDVCIEWLEGARAPQDLLR
                     RAKARREIVRESLEPVDLSQLEPPASAWKKNSGSLEQLRLRVLPKARRQVTKRLRGID
                     WNAAEERHRLRIAVKRLRYATDSLGGQTHTLERLQDQLGEHNDLAVARGL"
BASE COUNT          528 a         1104 c         1171 g          601 t
ORIGIN      
        1 ggcttcgaca tcctcgagtt cagccggcct tagcgccgaa tacgctttac tcgggctttg
       61 cgcccgaatc tttcactacc ttcgcccact ttggaatttc gcgcgcgatg tagtcggcga
      121 attccttcgg cgtgcccgtc gcgatttccg caccgagaga attcagacga tctttcaggt
      181 ctttttcttt cagaaccgca ttgatcgctt cattcagaaa ctgaacggtg ggcaaaggcg
      241 ttcctgcggg aacgaccacg ccatgccagg tggtcgcttc gaagcccggc acgccggctt
      301 cggccacggt cggcagctcg gggacgctcg cggaacgctg cgcggtggta acggcaaggg
      361 cgcggaggcg gccggctttg atgtagggga tgacggaggc ggaggtggcg aacatcatct
      421 gcacctgccc ggcgatcacg tcagtcagcg cgggctgggc gcccttgtag gggacatgca
      481 cgaggtcgac gccggccatc gccttgaaga gctcgcccgc gagatgcgcc gcggcgccgc
      541 tgcccgagga ggcgtagttg agctttcccg gattcgcctt tgaaaattga atcaattctt
      601 ctacggaaga aacgggcagg ctcggatgaa cgaccaggat attgggctgg atcgccacca
      661 gcgcgaccgg cgcgaaatcc ttgaccggat cgtaggggag gctgcggtag aggctctggt
      721 tggtggcgag gatgctgttg ttgccgagga gccaggtgtg gccgtcgggc gcgcttttcg
      781 cggcggcctc ggcggcgagg ttgccgccgg cgccggggcg gttgtcgacc acgacctgct
      841 gcgaccagcg tttcgcgagc ttctggccga gggcgcgcgc gaggatgtcg ctcgggccgc
      901 cgggcgtgaa cccgaccaca aaacgtaccg gcttggtggg gaagccctgg ccgaaggcgc
      961 tgcccgacgc cagaatgacg gcgcaaagaa aagccagagc ccgggccatg gattcccgct
     1021 gtcgcgggaa gaacggcgcg attacttcgc tacctggcgc tcgcgaagct cgtccagcgt
     1081 cttgcagtcg atgcacagcg tcgcggttgg ccgggcttcc aggcgcttga ggccgatctc
     1141 gacgccgcag ctgtcgcagt agccgtactc gtcggcgtcg attctcgcga tggtctcgtc
     1201 gatcttcttg atcagcttgc gctcgcggtc gcggttgcgc agctcgagcg ccatgtcgga
     1261 ctcctggctg gcgcggtcgt tgggatcggc gaacaccgtg gcctcgtcct gcatggtgtg
     1321 cacggtgcgg tcgatctcct ccgagagctc ggacttgagc gtctccagga tcttgcggaa
     1381 gtgcttgcgc tgcgcgccgt tcatgtactc ctcgcccttt tttgctgcgt agggcgtgaa
     1441 gcctttgcgt gtcagttcta cggccattct gcggctaccc ctgaaaaagc gaggcgactt
     1501 tgtatcagaa aagaggccct tgcggcaagc aagtaaattg cccccagcat gtgcccagag
     1561 tatcatcccc ccggtttctc ggggtgtagc gcagcctggt agcgcgcttg ctttgggagc
     1621 aagatgtcgg gagttcaaat ctccccaccc cgaccaggtt ttcgcccgac ggctgcccgt
     1681 agctcaaccg gatagagcac cagccttcta agctgggggt tgtgggttcg agtcccgccg
     1741 ggcaggccac agccaggtga taaaatcgcc gccccgctgc acgtgcgcga cgacggtgag
     1801 cgtagctcag ttggttagag catcggtttg tgaagccgag ggtcgtgggt tcaattccca
     1861 tcgctcaccc caaattccct ccacgccgag gcgctgacgg catgcgcttc gccgtcgcga
     1921 cgcccgaccc tgccgcctgg ctccatgtcc gggctttcga cgaggtggcc gaggcgctct
     1981 gcgagggctt gcgcgacctg ggccacgcct gcgagcgcct gacaggctcg ctgtcgagcg
     2041 acggcaccaa catcctgttc ggcgcgcacc acctgtcgcg cgcgttcgtg ccgccgccgg
     2101 gcacgatcct cttcaacctc gagcagatcg tgccggggtc gctctgggtt tcgccggact
     2161 atcttgcgct gctgcggcaa taccgggtct gggactacag cgctcggaac gtcgaagcgc
     2221 tgcgcgcggc cggcgtcgcg cgcgtctcgc atgcgccgct cggctacgtt gcaagccttg
     2281 aacgcatcgt ggcgcaggcc gatgacatcg acgtactctt ctacggcagg ctcatcccgc
     2341 ggcgcgtcga gttgctcgat cggatgagcg cgtacggcat gcgcgtggtt gcggtgaacg
     2401 acgcctacgc ggaagcgcgc gacgcgctca tcgcaagcgc gaaagtaatc cttaacgtcc
     2461 acgtggcgga ggaggcgcgc ctggaggcgg cgcgctgctt ctacgcgatg atcaacgggc
     2521 gcttcgtgct ctcggaggac agccccgacg cgattgagag cgggctggcc ggcggaatgg
     2581 cgtttgccgc ctacggcgac ctgctcgagc gctgcgcctg gtacctcgcg cggcccgccg
     2641 agagggcggc ggtggcgcac gccggccgca gcctcatgcg cgcgcgcccg caggccgcgc
     2701 tgctacggcc ggcactggcc gagctcgaga cgtgagccgg ttcgcgtctc cccgcaagtg
     2761 ggttgcgcct cgtttgcgcg aggggaccag cgcgagcgcc gccgccggcg ccatcctgca
     2821 ggcggcgctc gcccaggcga gaagcaatgc gcgcggcgcc atcgactcgc cgcatgcgga
     2881 gcacctgcac cagctgcgtg tcggcctgcg acgctatcgc tcggcgctgc gcctgttccg
     2941 cgacctcctg cgaaaaaagc cgcggcggag gctggcgcga cgctcgcgca aggcaatgga
     3001 gccgctcggc ccggtacgcg actgggacgt gtgcatcgaa tggctcgagg gggcgagggc
     3061 gccgcaggac ctgctgcggc gggccaaggc gcgccgcgag atcgtccggg aatcgctcga
     3121 gccggtcgac ctttcgcagc tcgagccccc ggcgtcggcg tggaagaaaa attccgggtc
     3181 gctggaacag ctccgcctgc gggtcctgcc caaggcgcgc cgccaggtga cgaagcgctt
     3241 gcgcggcatc gactggaacg cggccgaaga gcggcaccgg ctgcgaatcg cggtcaagcg
     3301 cctgcgctac gccaccgact ccctcggcgg ccagacgcac acgctcgagc ggctgcagga
     3361 ccagttgggc gagcacaacg atcttgcggt tgcgcgcggc cttc
//
LOCUS       DASYJA010000273         3777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3129112, whole genome shotgun sequence.
ACCESSION   DASYJA010000273 DASYJA010000000
VERSION     DASYJA010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3129112"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="VGO02_05860"
     CDS             <1..1143
                     /locus_tag="VGO02_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7477247.1"
                     /translation="RVVFSREKESIPYWNKFLQGYFDSSAIPSDTFDQAVRISLEGDA
                     GLTPEMESRGIRLRTSVDTSILYFGFNWLDSVVGGSSERSRKLRQAISIAVDYEEFIS
                     IFLNGRGLVAQDPLAPGIFGQHAGREAINPVTHEWRNGAARRRPIEEARKLLAEAGYP
                     DGRNAKTGEPLVLYLDTVGRGPGDKPHFDWWRRQFGKLAIQLEIRDTDWNRFQEKIRK
                     GSQQLFILGWNADYPDPENFLFLLHGPQSRMKSQGENASNYLNPEFDALFERVKNIPN
                     SAERQRVIDRMVDIARRDAPWLWGYHPKTYGLSHAWMQNGKPNNMARNNLKYLRVDVA
                     KREAMRREWNRPVIWPAVLVLVVLVLSAVPAFLAYRRRERMAARPA"
     gene            1144..2106
                     /locus_tag="VGO02_05865"
     CDS             1144..2106
                     /locus_tag="VGO02_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7477248.1"
                     /translation="MLGYVVRRVLYAIPILIGINLFTFVLFFLVNTPDDMARMQLGQK
                     RVTPEAIQKWKAERGYDKPLLYNQGSFTNTIFFEKSVRLFAFDFGRAEDNRDIGSEIR
                     QRMGPSLALAIPVFLVGLALNITFALGMAFFRATYLDVWGVVLCVAMMSISALFYIIG
                     GQYLISKLWHLVPISGYAGGADAAKFIVLPALIGVVGGIGAGSRWYRTLFLEEMTRDY
                     VRTARAKGLAESAVMFRHVLKNAMIPILTGVVAVIPLLFLGSLLSESFFGIPGLGSYT
                     IDAIQAQDFAVVRAMVFIGSVLYIFGLLLTDLSYTLVDPRVRLD"
     gene            2107..3459
                     /locus_tag="VGO02_05870"
     CDS             2107..3459
                     /locus_tag="VGO02_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7477249.1"
                     /translation="MPLVLATDLLIWLLVAAAAAFAWYCARRPHLAAPWARVFRSRAA
                     IASSVFLFFYLAIGLLDSLHYRPALASKDAGARVAYGVEVRSLLDLTLTSLRERSERT
                     YSAPLATRLFQKEQVEQPDGKSVREFPRLRFGGVHLKDETNLNQDIALRVLAGLVAGL
                     VLWFVFYISTRKSLARRWPQVPWPAAFAALAALLAIAGPVALLAAKYHVLGTDKIGQD
                     VLYQALKSIRTSLVIGTLTTLVLLPVGITAGIAAGYFRGRIDDAIQYIYTTLNSIPGV
                     LLIAASVLMIQVFLDQHATWFATAAQRADMRLLALCAILGLTSWTGLARLLRGEALKL
                     SQLEYIQAAHAFGVSHARILSRHILPNVTHIVVIALVMDFSGLVLAEAVLSYVGVGVD
                     PSTTSFGTMINAARLEMARDPMVWWSLGTAFVFMFALVLAANLFADAVRDGFDPRVRV
                     "
     gene            3456..>3777
                     /locus_tag="VGO02_05875"
     CDS             3456..>3777
                     /locus_tag="VGO02_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7477250.1"
                     /translation="MKVENLSTVLDVPAGELRAVDGISFELKAGECFALVGESGCGKS
                     MTALSLMRLLPEAGRIAGGRVELGGVDLLGLPEAAMRAVRGKRVSMIFQEPATALNPV
                     LTVGA"
BASE COUNT          574 a         1261 c         1246 g          696 t
ORIGIN      
        1 cgggtcgtgt tctcgcgcga gaaggagagc atcccctact ggaacaagtt cctgcagggc
       61 tacttcgatt cgtcggccat cccgagcgat accttcgacc aggcggtgcg catttcgctg
      121 gagggcgacg ccgggctcac gccggagatg gaaagccgcg gcatccggct gcgcacctcg
      181 gtggacacca gcatcctgta tttcggcttc aactggctcg acagcgtggt tggcggctcc
      241 tccgagcgtt cccgcaagct gcgacaggca atctccatcg cggtggacta cgaggagttc
      301 atctcgatct tcctcaacgg ccgcggcctg gtggcgcagg atccgctcgc gccgggcatc
      361 ttcgggcagc acgcggggcg cgaggcgatc aacccggtga cgcacgagtg gcgcaacggc
      421 gcggcgcgcc ggcggccgat cgaggaggcg cgcaagctgc tcgccgaggc gggctatccc
      481 gatgggcgca acgcgaagac cggcgagccg ctggtgctgt acctcgacac cgtcggccgc
      541 ggcccgggcg acaagccgca cttcgactgg tggcgccgcc agttcggcaa gctcgccatc
      601 cagctcgaga tccgcgacac cgactggaac cgcttccagg agaagatccg caagggcagc
      661 cagcagctct tcatcctagg ctggaacgcc gactatcctg acccggagaa cttcctgttc
      721 ctgctgcacg gcccgcaatc gaggatgaag agccagggcg agaacgccag caactacctc
      781 aacccggaat tcgacgcgct gttcgagcgc gtcaagaaca tccccaattc cgccgagcgc
      841 cagcgcgtga tcgaccgcat ggtggacatc gcgcggcgcg acgccccctg gctgtggggc
      901 taccacccga agacctacgg cctgtcgcac gcctggatgc agaacggcaa gcccaacaac
      961 atggcgcgca acaacctcaa gtacctgcgg gtcgacgtgg cgaagcgcga ggcgatgcgc
     1021 cgggaatgga accggccggt aatatggcct gcggtcctgg ttcttgtagt tttggtgtta
     1081 agcgctgttc ctgcttttct tgcctataga cggcgtgaac gcatggcggc gcggccggca
     1141 tagatgctcg gctacgtcgt ccgccgcgtt ctgtacgcga tcccgatcct gatcgggatc
     1201 aatctgttca ccttcgtcct gttcttcctg gtcaatacgc cggacgacat ggcgcgcatg
     1261 cagctcggcc agaagcgcgt gacgcccgag gcgatccaga agtggaaagc cgaacgcggg
     1321 tacgacaagc cccttctcta caaccagggg tctttcacga acacgatttt ttttgaaaag
     1381 tcggtaaggc tgtttgcctt cgacttcggc cgcgccgagg ataaccgcga catcggcagc
     1441 gagatccgcc agcggatggg gcccagcctg gccctcgcca tcccggtgtt cctggtcggc
     1501 ctggcgctga acatcacctt cgcgctcggc atggcgttct tccgcgccac ctacctggac
     1561 gtgtggggcg tggtgctgtg cgtggcgatg atgtcgatca gcgcgctttt ctacatcatc
     1621 ggcgggcaat atctgatttc gaagctgtgg cacctggtgc ccatctcggg ctacgccggc
     1681 ggcgccgacg cggcgaagtt catcgtgctg cccgcgctca tcggcgtggt gggcggcatc
     1741 ggcgcgggca gccgctggta ccgcacgctg ttcctcgagg agatgacgcg cgactatgta
     1801 cgcactgcgc gcgcgaaggg cctcgccgag tcggcggtga tgttccgcca cgtgctcaag
     1861 aacgcgatga tcccgatcct caccggggtg gtggcggtga tcccgctcct gttcttgggc
     1921 agcctgctgt cggaatcctt cttcggcatt ccgggcctgg gcagctatac catcgatgcc
     1981 atccaggcgc aggacttcgc ggttgtgcgc gccatggtgt tcatcggctc ggtgctctac
     2041 atcttcggcc tgctgctcac cgacctttcg tacacgctgg tcgatccgcg cgtgaggctc
     2101 gactagatgc cgctcgtcct cgccacggac ctgctgatct ggctgctggt ggcggcggcc
     2161 gcggcgttcg cctggtattg cgcgcggcgc ccgcacctgg ccgctccgtg ggcgcgggta
     2221 ttcaggagcc gggccgcaat cgcttccagt gttttcctgt ttttctacct ggccatcggg
     2281 ctgctcgatt cgctgcacta ccgacccgcg ctggcatcga aggacgccgg cgcccgggtg
     2341 gcgtacggcg tggaagtgcg ctcgctcctc gacctgacgc tcacctcgct gcgcgagagg
     2401 tccgagcgca cctactcggc gcctctggcg acccgcctgt tccagaaaga gcaggtggag
     2461 cagcccgacg gcaaatcggt gcgcgaattt ccccgcttgc gcttcggtgg tgtgcacctc
     2521 aaggatgaaa caaacttgaa ccaggacatc gccctgcgcg tgctagccgg actggtggcc
     2581 ggactcgtcc tctggtttgt tttctacatc agtactagaa aatccctggc gcgccgctgg
     2641 ccgcaggttc cctggccggc agcgtttgcc gcgctggccg cgctgcttgc catagccggg
     2701 ccggtagcgc tgctcgccgc caaataccac gtcctcggca ccgacaagat cgggcaggac
     2761 gtgctctacc aggcgctgaa gagcattcgc accagcctgg tcatcggcac gctcaccacg
     2821 ctggtgctgc tcccggtggg catcacggcc ggtatcgccg cgggctactt ccgcggccgg
     2881 atcgacgatg cgatccagta catctatacg acgctcaact cgatccccgg cgtgctgctc
     2941 atcgccgcct ccgtgctgat gatccaggtg ttcctcgacc agcacgcgac ctggttcgcg
     3001 accgccgcgc agcgcgccga catgcggctg ctggccttgt gcgcgatcct gggcctgacc
     3061 agctggaccg ggctggcacg gctgctgcgc ggggaggcgc tcaagctctc gcagctcgag
     3121 tacatccagg cggcgcacgc cttcggcgtc tcgcacgcgc gcatcctgtc gcgccacatc
     3181 ctgcccaacg tgacgcacat cgtggtgatc gcgctggtga tggacttctc gggcctggtg
     3241 ctggccgagg cggtgctgtc ctacgtcggc gtgggcgtcg atccctcgac caccagcttc
     3301 ggcacgatga tcaacgccgc gcgcctggag atggcgcgcg acccgatggt gtggtggtcg
     3361 ctcggcaccg ccttcgtgtt catgttcgcg ctggtgctgg cggccaacct cttcgccgac
     3421 gcggtgcgcg acggcttcga tccgcgggtg cgggtatgaa ggtcgagaac ctctcgacgg
     3481 tcctcgacgt gccggccggc gagctgcgcg cggtcgacgg catcagcttc gagctgaagg
     3541 ccggcgagtg cttcgcgctg gtgggcgagt cgggctgcgg caagtcgatg accgcgctgt
     3601 cgctgatgcg gctgctgccc gaggcggggc gcatcgccgg cggccgcgtg gagctcggcg
     3661 gcgtcgacct cctcggcctg cccgaggcgg cgatgcgggc ggtgcgcggc aagcgcgtgt
     3721 cgatgatctt ccaggagccg gcgaccgcat tgaatccggt tctcacggtg ggcgccc
//
LOCUS       DASYJA010000274         1663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2764573, whole genome shotgun sequence.
ACCESSION   DASYJA010000274 DASYJA010000000
VERSION     DASYJA010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2764573"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..451)
                     /locus_tag="VGO02_05880"
     CDS             complement(65..451)
                     /locus_tag="VGO02_05880"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEV7477251.1"
                     /translation="MKPELMWLLWAVALTVVQMLIAVTGATLQVGLPRLAGNRDGMPP
                     LTGWVGRAERAHYNMLESLVLFAALVVIAVLASKTNSTTLMAAQLFVWARLAYAVIYL
                     AGIPWLRTLVWFVSMIGLVIIFCQLV"
     gene            complement(493..864)
                     /locus_tag="VGO02_05885"
     CDS             complement(493..864)
                     /locus_tag="VGO02_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477252.1"
                     /translation="MKEIFGVADDPPARRRWFHDDYFDLFVWQADGEVTLFQLCYGAD
                     SAERAVVWDKSRGFFHDGPPAANDDVFTRFEEAAANLPEDVRREVREKMHEFAGRRLA
                     VRSRRKQFRRDTWQRGSATRR"
     gene            1017..>1663
                     /locus_tag="VGO02_05890"
     CDS             1017..>1663
                     /locus_tag="VGO02_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HEV7477253.1"
                     /translation="MNSPISKPHSDLLKSLSEFSKEHRAARWSGTFAAFLEGIFPADP
                     PGLTRSSHQYLWDMMRANDRLFNDELYGIDGTIERVLDYFKAAAAGSEVGRRLLLLLG
                     PPSGGKSSMVILLKRGLEEYSLTDAGALYAIHGCPVHESPLHLVPHTLRAQFRSTYGV
                     EIGGELCPHCASRLAQEYGGDFMQMPVQRIFASEAGRIGIGTYAPHDPSTADIADL"
BASE COUNT          294 a          575 c          533 g          261 t
ORIGIN      
        1 ctgccgccgc gcgagaaagg cgcggcgggc ggcgtgatcc ggccggtcgc gatcggcgcg
       61 ccggctacac cagctggcag aaaatgatca ccaggccgat catcgagacg aaccagacca
      121 gcgtgcgcag ccaggggatg ccggcgaggt agatgacggc gtaggcgagg cgcgcccaga
      181 cgaaaagctg cgccgccatc agcgtggtgg agttggtctt gctggcgagc acggcaatca
      241 cgaccagtgc cgcgaacagc accaggcttt ccagcatgtt gtagtgcgcg cgctcggcgc
      301 gccccaccca gccggtgagc ggcggcatgc catcgcggtt gccggccagc ctcggcaggc
      361 ccacctgcag ggtcgcgccg gtcacggcaa tcagcatctg caccacggtc aaagccacag
      421 cccacagcag ccacatcagc tcaggtttca tgcgacctcc tcccttgaag agaggctatt
      481 gtcgctgaat ccctacctgc gtgtcgccga gccccgctgc caggtgtcgc ggcggaattg
      541 cttgcggcgc gagcgcaccg cgaggcggcg cccggcgaac tcgtgcatct tctcgcgcac
      601 ctcgcggcgc acgtcctccg gcaggttcgc cgccgcctcc tcgaagcgcg tgaacacgtc
      661 gtcgttcgcg gcgggcggac cgtcatggaa gaagccgcgg ctcttgtccc agaccacggc
      721 gcgctcggcc gagtcggccc cgtaacagag ctggaacagc gtcacctcgc cgtcggcctg
      781 ccagacgaac aggtcgaaat agtcgtcgtg gaaccagcgt cggcgcgcgg gcgggtcgtc
      841 ggcaactccg aagatctctt tcatccctga ccttttcccg tattgcacgg ttgttgcgtc
      901 aacgttttga ccacaggtcc gttcagaggg ttcatgcggt tacaaaaatg atggatcgcg
      961 ggaggcgaac catgttctaa cggcaaacgg ggatattccc catcgagagg tgctgcatga
     1021 acagcccgat ttcgaagcct cactcggact tgctcaagtc gctgagcgaa ttcagcaagg
     1081 agcatcgcgc ggcacgctgg tcgggaacct tcgccgcgtt tctcgaaggg attttcccgg
     1141 cagacccgcc cggcctgacg cgctccagcc accagtacct gtgggacatg atgcgcgcca
     1201 acgatcggct gttcaacgac gagctgtacg gaatcgacgg cacgatcgag cgggtgctcg
     1261 actacttcaa ggccgccgcc gccggctccg aggtcggcag gcgcctgttg ctgctgctcg
     1321 gcccgccctc gggcggcaaa tcgagcatgg tgatcctgct caagcgcggc ctcgaggaat
     1381 acagcctcac cgatgccggc gcgctctacg cgatccacgg ctgcccggtg cacgaatcgc
     1441 cgctgcacct cgtgccgcac acgctgcgcg cgcagttccg cagcacctac ggcgtcgaga
     1501 tcggcggcga gctctgcccg cactgcgcga gccgcctagc gcaggagtac ggcggcgact
     1561 tcatgcagat gccggtgcag cgcatcttcg cctccgaggc cggccgcatc ggcatcggca
     1621 cctacgcgcc gcacgacccg agcaccgccg acatcgcgga cct
//
LOCUS       DASYJA010000275         3958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2310690, whole genome shotgun sequence.
ACCESSION   DASYJA010000275 DASYJA010000000
VERSION     DASYJA010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2310690"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /gene="pth"
                     /locus_tag="VGO02_05895"
     CDS             <1..581
                     /gene="pth"
                     /locus_tag="VGO02_05895"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEV7477254.1"
                     /translation="GRMQREHAGDSAWLLLPQSFMNSSGRAVQMLAGFFKIKPEEILV
                     VHDELDFPPGTARIKQGGGIAGHNGLKDISQRIGSHDYWRLRLGVGRPPAGTEGGDYV
                     LHKPSAEERAAIDASIENALGVLPQCIAGDLQGAMQKLHSQDRPAPQREPEKKESPKK
                     EPPKKEPEKKAPAVKEPPKEEKGFLKSLFGKK"
     gene            578..1660
                     /gene="ychF"
                     /locus_tag="VGO02_05900"
     CDS             578..1660
                     /gene="ychF"
                     /locus_tag="VGO02_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEV7477255.1"
                     /translation="MKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGIVTV
                     PDPRLDVIAGIEKPQKVVPAIVEFVDIAGLVAGASKGEGLGNKFLANIRETDAIAHVV
                     RCFEDPNVVHVAGKLDPVSDIETINTELALADLDAVEKQISKFAKVAQTTGEKDAVRL
                     VAALQKVRAVLDAGKPARSAELSKEEHAVLRPLFLLTMKPTMYVANVAEKGSDALLQK
                     VISYAAKEKAPVVPISAAIESQIADMSDEDKTVFLDDLGMKEPGLNRVTRAAYALLGL
                     QTYFTAGPKEVRAWTVRAGATAPQAAGVIHTDFERGFIRAEVASYDDFVAHKGELGAK
                     EAGKLRLEGKDYVVRDGDVVHFRFNV"
     gene            1657..2106
                     /locus_tag="VGO02_05905"
     CDS             1657..2106
                     /locus_tag="VGO02_05905"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7477256.1"
                     /translation="MSVELGVATRLDLPQLVALLGVLFSQEAEFVPDDTKQTRALEKI
                     LADDSVGRIHVARDGGTVVAMASLIYGISTAEGGLAASFEDFVVLPGYRGKGIGAELL
                     QYVIAEARKQGVLRLILLTDRQNERAQALYRKLGFADSTMKAMRLKL"
     gene            2103..2567
                     /locus_tag="VGO02_05910"
     CDS             2103..2567
                     /locus_tag="VGO02_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEV7477257.1"
                     /translation="MRILLMLAALAAAPAFAQQQADPQVNRGRYLIKIGGCNDCHTPN
                     YPASGGKVPEAQWLTGDALGWNGPWGTTYATNLRLYMQDLTEDQWVKKAKTLQARPPM
                     PWFNVRAMSAGDLRAMHRYIRSLGPAGKPAPAHLPPDKTPPEPAVRFPAPPK"
     gene            complement(2542..3537)
                     /locus_tag="VGO02_05915"
     CDS             complement(2542..3537)
                     /locus_tag="VGO02_05915"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HEV7477258.1"
                     /translation="MKGEEFKDDFRRALPRELVLELTRRSAWRASLAVLQDFAVIAVA
                     LAAALAYWPNPLVIFLSVLVIGTRQHALFVIAHDAAHYLLFESRKANDLVGRACATVQ
                     GLSMCTYRVIHRLHHNNLYGELDPDTALHGGYPRGTWYLIRKLLKDLAGLTAWKTYAY
                     FLGGAPALNSATNVALRPLDDTSGKLRSEARSDRNTVIVFHIVLLVLFAWSGYIVQYV
                     VLWILPLVTVVQAILRLRAISEHGATTDFSSPLTAARTNVCPAWLEWLVFPHHVNYHI
                     EHHLYASVPQYNLPRLHREMAVRGVLEGAEVVPFRLTLEKIFCERRTATSAAPGT"
     gene            complement(3548..>3958)
                     /locus_tag="VGO02_05920"
     CDS             complement(3548..>3958)
                     /locus_tag="VGO02_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV7477259.1"
                     /translation="LAKGAKVSVGGKPHQLGGFFFQPTVLTGVTPEMLISHEETFGPV
                     APLIRFKTEEEAIRLANASEFGLAGYFYSRDIGRIWRVAEAMEAGMIGVNAGIISTEV
                     APFGGVKSSGLGREGSKYGIEEYLEIKYVLMGGL"
BASE COUNT          747 a         1270 c         1322 g          619 t
ORIGIN      
        1 tgggtcgcat gcagagggaa catgccggcg acagcgcctg gttgctcctg ccgcaaagct
       61 tcatgaattc gagcggccgg gcggtgcaga tgctggcggg attcttcaag atcaagcctg
      121 aagagatcct ggtggtgcac gacgagctcg acttccctcc cgggacggcg cgcatcaagc
      181 agggcggcgg catcgccggc cacaacggct tgaaggacat ctcgcagcgc atcggcagcc
      241 acgattactg gcgcctgcgg ctgggtgtgg gcaggccgcc ggcgggcacc gagggcggcg
      301 actacgtgct gcacaagccg tcggccgagg agcgtgcggc gatcgacgcg tcgatcgaga
      361 acgcgctcgg tgtgctgccg cagtgcatcg ccggagatct gcaaggcgcg atgcagaagc
      421 tgcattccca ggaccgcccg gcgccacaga gagaacccga gaagaaggag tcgccgaaga
      481 aggagccgcc gaagaaggag cccgagaaga aggcgcctgc cgtcaaggaa ccgccaaagg
      541 aagaaaaagg attcctcaaa tccttgttcg gtaagaaatg aagtgcggca tcgtcggctt
      601 gcccaacgtc ggcaagtcca cgctcttcaa cgccctcacc aaggcgggca tcgcggccga
      661 gaactaccct ttctgcacca tcgagccgaa tgtcggcatc gtcacggtgc ccgacccgcg
      721 cctcgacgtg atcgccggaa tcgagaagcc gcagaaagtc gtgccggcga tcgtcgagtt
      781 cgtcgacatt gccggcctgg tggccggtgc ctccaaaggc gagggcctgg gcaacaagtt
      841 cctcgccaac atccgcgaga ccgacgccat cgcgcacgtc gtgcgctgct tcgaggaccc
      901 gaatgtggtc cacgtcgccg gcaagctcga cccggtttcg gacatcgaaa ccatcaacac
      961 cgagctcgcg cttgccgacc tcgatgccgt ggagaagcag atttcgaagt tcgccaaggt
     1021 ggcgcaaacc accggcgaga aggatgccgt gcggctggtc gcggcgctgc agaaggtgcg
     1081 cgcggtgctc gatgccggca agccggcgcg cagcgcggag ctgtccaagg aagagcatgc
     1141 ggtcctgcgg ccgcttttcc tcctgacgat gaagccgacg atgtatgttg ccaacgtggc
     1201 ggagaagggc tcggacgctc ttctgcaaaa agtaatctcg tatgcggcaa aagagaaagc
     1261 cccggtggtg ccgatcagcg cggcgatcga gtcgcagatc gccgacatgt ccgacgagga
     1321 caagacggtg ttcctcgacg acctgggcat gaaggagccg ggccttaacc gcgtcacccg
     1381 cgccgcttac gcgctgctcg ggctgcagac gtacttcacc gccgggccga aggaggtgcg
     1441 ggcgtggacg gtgcgcgccg gcgccaccgc gccgcaggcc gccggcgtca tccacaccga
     1501 cttcgagcgc ggcttcatcc gcgccgaggt ggcgtcctac gacgatttcg tcgcgcacaa
     1561 gggcgagctg ggtgccaagg aggcgggcaa gcttcgcctc gaaggcaagg actacgtggt
     1621 gcgtgacggc gacgtggtcc acttccgctt caacgtgtga gcgtcgagct cggagtcgcg
     1681 acgcgcctgg acctgccgca gctggtcgcg ctgctcggcg tgctgttctc ccaggaagcc
     1741 gagttcgtcc ccgacgacac caagcagacg cgcgcgctgg agaagatcct cgccgacgat
     1801 tcagtgggtc gcatccatgt cgcacgcgac ggcggcactg tggtcgcgat ggcgagcctg
     1861 atctacggca tcagcaccgc ggagggcggc ctggcggcct cgttcgagga cttcgtggtg
     1921 ctgccgggct accgcggcaa aggcatcggc gccgagctgc tgcaatatgt gatcgccgag
     1981 gcgcgcaagc agggcgtgtt gcgcctgatc ctgctgaccg accggcaaaa cgagcgcgca
     2041 caggcgctct accgcaagct aggattcgcc gactccacca tgaaagcgat gaggctcaag
     2101 ctatgagaat tctgctcatg ctcgcggcgc tcgccgccgc tccggccttc gcgcagcagc
     2161 aggccgaccc tcaggtgaac cgcggccgct acctgatcaa gatcggtggc tgcaacgact
     2221 gccacacccc caactatccg gcgagcggcg gcaaggtgcc cgaggcgcaa tggctgaccg
     2281 gagacgcgct cggctggaac gggccgtggg gaaccactta cgcgaccaac ctgcgcctct
     2341 acatgcagga cctgaccgaa gatcagtggg tgaagaaggc gaagaccctg caggcgcggc
     2401 cgcccatgcc gtggttcaac gtgcgggcga tgagcgcggg cgatctgcgc gccatgcatc
     2461 gctatatccg cagcctcggg cccgccggca agccggcgcc ggcccacctg ccgccggaca
     2521 agacgccgcc cgagccggcg gtcaggttcc cggcgccgcc gaagtagcgg tccgcctttc
     2581 gcagaaaatt ttctccagcg taaggcggaa aggcaccacc tcggcaccct ccagcacgcc
     2641 gcgcaccgcc atctcgcgat gcagccgcgg caggttgtac tgcggcaccg aggcgtaaag
     2701 gtggtgctcg atgtgatagt tgacgtggtg cgggaagacc agccactcga gccatgccgg
     2761 gcagacattc gtgcgcgcgg cggtgagcgg cgacgagaag tccgtggtgg cgccgtgctc
     2821 ggagatcgcc cgcagcctga ggatcgcctg taccacggtg accagcggca ggatccagag
     2881 caccacgtac tgcacgatat agccgctcca ggcgaacaag acgagcagca cgatgtggaa
     2941 aacgatcacc gtgtttcggt cggatctcgc ctcgcttctc aatttcccgg aagtatcatc
     3001 gagcggccgg agggccacat tcgtggctga gttcagggcg ggggcgccgc cgaggaagta
     3061 cgcgtaggtc ttccaggcgg tcaggccggc aaggtctttc agaagctttc gaatcaaata
     3121 ccacgtgccg cgcggatagc cgccgtgcag cgcggtgtcc ggatcgagct cgccgtacag
     3181 gttgttgtga tgcaggcggt ggatcacccg gtaggtgcac atcgacagcc cctgcacggt
     3241 cgcgcaggcg cgccccacga ggtcgttcgc ttttctgctt tcgaacagca ggtagtgcgc
     3301 ggcgtcgtgc gcgatgacga acagggcgtg ctgccgcgtg ccgatcacga gcacggatag
     3361 aaaaatcacc agtggattcg gccagtaggc aagcgcagcg gccagtgcta ctgcaataac
     3421 tgcaaagtcc tgtagcacgg caagactggc gcgccatgcg gatcggcgcg tgagctccag
     3481 gaccagctcc cgcggcaggg ccctgcggaa gtcgtccttg aactcttcgc ctttcacctc
     3541 attctcctca caggccgccc atcagcacgt acttgatctc gaggtattcc tcgatgccgt
     3601 acttggagcc ttcgcggccg aggcccgacg acttcacgcc gccgaagggc gcgacttcgg
     3661 tcgagatgat tccggcgttg acgccgatca tgccggcctc catcgcctcg gcgacgcgcc
     3721 agatgcggcc gatgtcgcgg ctgtagaagt aaccggcgag gccgaactcg ctcgcgtttg
     3781 cgaggcggat cgcttcttcc tcggtcttga agcggatcag tggcgcgacc ggcccgaacg
     3841 tctcctcgtg cgagatcagc atttcgggcg tgacgccggt cagcaccgtc ggctggaaga
     3901 aaaagccgcc gagctgatgc ggcttgccgc cgacgctgac cttcgcgccc ttggcgag
//
LOCUS       DASYJA010000276         2829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_496574, whole genome shotgun sequence.
ACCESSION   DASYJA010000276 DASYJA010000000
VERSION     DASYJA010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496574"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="VGO02_05925"
     CDS             <1..950
                     /locus_tag="VGO02_05925"
                     /inference="COORDINATES: protein motif:HMM:NF022073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0047989 - hydroxybutyrate-dimer hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxybutyrate oligomer hydrolase family
                     protein"
                     /protein_id="HEV7477260.1"
                     /translation="PLAAFPIHFYANDPGQLFARWTEFMIKAGRIAQDGVKAAYGRSP
                     RFTYAVGVSNGGYQVRRAIEAAPEVFDGGVDWEGTFVDDAAPNILTDLPPAVLNFPDY
                     RASGFNSASTAAANIRGAGYPPDIVAGPASLWANHWASFWEVTQCQWQKRHDPAYDTY
                     GSGTGTYNYVSRLSFSDVGAQLAAFANTGEVRQPLVTVAGTMDALLPIDHHARAYARK
                     VASASKQSKHDDDDDDRADRRSPYRLYEIQNGNHLETYKDTFTQLEFIQPHAQRAFDL
                     VVEQVERHRSLPPSQCVARGGSISDTPAQAGHCASLFVP"
     gene            complement(947..2632)
                     /locus_tag="VGO02_05930"
     CDS             complement(947..2632)
                     /locus_tag="VGO02_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477261.1"
                     /translation="MGRFQQENSGHKEDPASREPAGWLLAAALLAVIVFGYLMPVTAY
                     FSAVPGDFGDPRYNSVVLEHLYRVVTGAYKAGLWNPDFFYPFQGMLAFSDNHLGSGAT
                     YVVARLLGLSREHAFDVWFSVGTLLNFASALYVLRRLGLSTAAAALGAFFFAFAVPVP
                     AQDAHAQLVHRFAAPLAVLALWQMFERRRLADLGRLAFFTVWQFYCSIYLGLFLVYLL
                     AALSVALLTVRQPFEWAQWRANLAAERFSIKLGAGATLLGSALALAYLGRQYFAASQA
                     HQLLERLRPIEAITPAMPRLGSYLIADHSALLGWLSRGISVPDRSEHQMFIGFGASIL
                     IVTAALAAMRGRAAVPGLTQVMLIALALLFGGTLWFENMSLYQLVAWLPGIGAIRDVT
                     RVIFIMLVPMSVLVALGADAIRRRFGASAWTAAPVLAGVAALVIVEPLSVEKNGTPIA
                     QWQERLDAARALLPPVVPKDAILMVRTGSANLEERIFVELDAMLLGQDLGRPVLNGYS
                     AFVPPGYWIRPCASARDRLTGYSLFMRGVNASSYARRLIVLDLDNCPPALQVP"
BASE COUNT          515 a          911 c          985 g          418 t
ORIGIN      
        1 cccctttggc cgccttcccc atccatttct acgccaacga tcccggccag ctcttcgccc
       61 gttggacgga attcatgatc aaggccgggc gcatcgcgca agacggcgta aaagcggcat
      121 acggccgctc gccgcggttc acctacgccg tcggcgtctc gaacggcggc tatcaggtgc
      181 gccgggctat cgaggccgcg cccgaggttt tcgacggcgg cgtcgactgg gaaggcactt
      241 tcgtcgacga cgctgcgccg aacatcctca ccgacctgcc gcccgcggtc ctgaattttc
      301 ccgactaccg cgcttcgggg ttcaacagcg ccagcacggc ggcggcgaat attcgaggcg
      361 ccggctatcc cccggatatc gtcgccgggc cggcctcgct ctgggccaac cactgggcct
      421 cgttctggga agtgacgcag tgccaatggc agaagcgtca cgaccctgcg tacgacacct
      481 acggcagcgg caccggcacg tacaactacg tcagccgtct ttcgttctcg gacgtcggtg
      541 cgcagctggc cgctttcgcc aacacggggg aggtccggca gcctctggta accgtggccg
      601 gcacaatgga cgcgttgctg ccgatcgacc atcacgcgcg cgcctacgcg cgcaaggtcg
      661 ccagcgcatc gaagcaatcg aagcacgatg acgatgacga cgatcgcgcg gaccgacgct
      721 cgccgtaccg cttatacgag atccagaatg gcaatcacct cgagacgtac aaggatacgt
      781 ttacgcagct cgagtttatc cagccgcatg cgcagcgcgc gtttgatctg gtggtcgagc
      841 aagtggagcg gcatcggtcg ctgccgccca gccagtgtgt tgcacgcggt gggtcgatct
      901 ccgacacgcc ggcgcaggcg ggccactgcg cgagcctgtt cgtgccctag ggcacctgca
      961 gcgctggcgg gcagttatcc agatcgagca cgatcagccg gcgtgcatag ctcgatgcgt
     1021 tgacgccgcg catgaacagc gagtatccgg tcagccggtc tctggccgag gcgcatgggc
     1081 ggatccagta gcccggtggc acgaaagcgg agtagccgtt cagtaccggg cggcccagat
     1141 cctgcccgag tagcatcgcg tcgagctcga cgaagatccg ctcctcaaga ttggcggagc
     1201 cggtacgcac catgaggatc gcgtctttcg gcacgaccgg gggcagcagc gccctggccg
     1261 cgtcgaggcg ttcctgccat tgcgcgatcg gggtgccgtt cttctccacg ctgagcggct
     1321 cgactatcac cagggccgca acgccggcga ggaccggggc ggcggtccag gcgctcgctc
     1381 caaagcgacg ccgaatggca tcggcgccaa gcgccaccag caccgacatc ggtaccagca
     1441 tgatgaagat gacgcgggtg acatcgcgga ttgcgccgat gcccggcagc cacgcgacca
     1501 gctgatagag ggacatgttc tcgaaccaca gggtgccgcc gaacagcagc gccagggcga
     1561 tcagcatgac ttgagtcagg ccgggaaccg ccgcgcgccc gcgcatggcg gccagggcgg
     1621 cggtgacgat caggatgctc gcgccgaagc ctatgaacat ctggtgctcc gagcggtccg
     1681 gtacgctgat gccgcgggag agccagccga gcagcgcgct atggtcggct atgaggtagc
     1741 tccccaggcg cggcattgcc ggggtgatcg cctcgatagg gcgcaagcgc tcgagaagct
     1801 ggtgggcctg cgaggccgcg aagtattggc ggccgagata agccagcgca agagccgagc
     1861 cgagcagcgt cgcgcctgca ccaagcttga tcgagaagcg ctcggcggcg aggttggcgc
     1921 gccactgcgc ccattcgaag ggctggcgca cggtcaggag cgcgacgctg agcgcggcca
     1981 ggagatagac gagaaacagc ccaagataga tgctgcaata gaactgccag acggtgaaga
     2041 aggcgaggcg cccgagatcg gccaggcgcc gccgctcgaa catctgccac agcgccagca
     2101 cggcgagcgg ggcggcgaag cgatgcacca gctgggcatg ggcgtcctgc gccggcacgg
     2161 gcacggcgaa ggcgaagaag aaggcgccca gcgcggcagc ggcggtgctc aggcccaggc
     2221 ggcgcaggac gtagagcgcg gaggcgaaat tgagcagcgt tccgacgctg aaccagacgt
     2281 cgaaagcgtg ctcgcgcgac aggccgagca ggcgggcgac gacgtaggtg gcgccgctgc
     2341 cgaggtgatt gtcgctgaag gcgagcatgc cctggaacgg atagaagaaa tcggggttcc
     2401 agagccctgc cttgtaggcg ccggtgacga cgcggtacag gtgctccagc acgacgctgt
     2461 tgtagcgggg gtcgccgaaa tcccccggca ccgcgctgaa gtaggccgtg accggcatga
     2521 ggtagccgaa cacgatcacg gcgagcagcg ccgccgcgag cagccagccc gcgggctcac
     2581 gcgatgccgg atcttccttg tggcctgagt tttcttgctg gaatcttccc acccgcctct
     2641 tatagtacgc ggcgggcgcg cgcgcgagcc ttggcgctat gccggggcgt cgcccctggg
     2701 caggggttcg gggagggtgc tcttcaactt acgggcgcgt ttcgcgatgt cgagcgctct
     2761 tcggtagagc gcgacaagaa ggagcgttgg cagcggcgct gccggacgag ctggcgggct
     2821 gcgtctcac
//
LOCUS       DASYJA010000277         5993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1494533, whole genome shotgun sequence.
ACCESSION   DASYJA010000277 DASYJA010000000
VERSION     DASYJA010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494533"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..254
                     /locus_tag="VGO02_05935"
     CDS             51..254
                     /locus_tag="VGO02_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477262.1"
                     /translation="MRSPRRPDRRQSSDPAYRACCRPFQKPQRSIVPVDIYVPGCPPT
                     AEALLYGIVQLQNKIKRTNTIAR"
     gene            255..830
                     /locus_tag="VGO02_05940"
     CDS             255..830
                     /locus_tag="VGO02_05940"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEV7477263.1"
                     /translation="MTPRLQKLFDSIKDLNPVERLGELTVEVAASQYKSIAERLKSSG
                     FEQLIDLCGVDYASYGDKPREGNRFAAVIHLLSLKNNWRLRVRAFCPDDSFPVIDSLV
                     EVWPGVGWFEREAFDLFGIVFDGHPDLRRILTDYGFIGHPFRKDFPLSGHVEMRYDPE
                     QRRVIYQPVTIDPREVTPRVVREENYAGGDR"
     gene            830..2083
                     /locus_tag="VGO02_05945"
     CDS             830..2083
                     /locus_tag="VGO02_05945"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEV7477264.1"
                     /translation="MAEIKNYTLNFGPQHPAAHGVLRLVLELDGEVIQRADPHIGLLH
                     RATEKLAEHKTFIQSIPYMDRLDYVSMMCNEHAYVLAIEKLLGITPPLRAQYIRTMFD
                     EVTRILNHLLWLGAHGLDIGAMTIFLYCFREREDLMDAYEAVSGARMHAAYYRPGGVY
                     RDLPDSMPQYEVSKIKNSAEIQKLNQNRQGSLLDFLEDFTNRFPKHVDEYETLLTENR
                     IWKQRTVGIGVVSPERALALGFTGPMLRGSGIEWDLRKKQPYEVYDKVDFDIPVGANG
                     DCYDRYLVRVEEMRQSNRIVKQCIDWLRKNPGPVMVDNHKVAPPSRVDMKANMEELIH
                     HFKLFTEGMHVPEGECYAAVEAPKGEFGIYLISDGANKPYRLKIRAPGFAHLSALDEM
                     ARGHMIADAVAIIGTQDIVFGEVDR"
     gene            2083..2556
                     /gene="nuoE"
                     /locus_tag="VGO02_05950"
     CDS             2083..2556
                     /gene="nuoE"
                     /locus_tag="VGO02_05950"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEV7477265.1"
                     /translation="MLTTDSLKRIDREIAKYPPEQKKSAVMSALVIAQDEKGWLSNDL
                     MDYVAQYLGMAPVAVYEVASFYTMYNLKPTGRHKLTICTCLPCGLQGSLAAADHLREK
                     LGIGFNETTSDGRFTLKEGECMGACAMAPVVLVNNKKMHDYMSSEKLDQLLDSLK"
     gene            2553..3896
                     /gene="nuoF"
                     /locus_tag="VGO02_05955"
     CDS             2553..3896
                     /gene="nuoF"
                     /locus_tag="VGO02_05955"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEV7477266.1"
                     /translation="MKLDYGPDAILMKGLDGANWRLADYEKRDGYAALKKIISEKIKP
                     EDIIADLKKSALRGRGGAGFPTGLKWSFMPRAFPGAKYVVCNSDEGEPGTCKDRDLLR
                     YNPHSVIEGMIIAGYAMQASAGYNYIHGEIWETYELYEEALKEAYAAGYLGKNILGSE
                     FSYEMYAHPGYGAYICGEETALLESLEGKKGMPRFKPPFPASYGLYGKPTTINNTETF
                     AAVPWVIRNGGEAFLALGRPNNGGTKLFTVSGHVNRPGNYEVKLGTPFAKLLEMAGGV
                     RRGRKLKAVIPGGSSMPVLTAEVMMKTDMDYDSIAKAGSVLGSGGVIVMDDSTCMVRA
                     LERLSYFYFEESCGQCTPCREGTGWLYRVVHRIEEGLGRMEDLALLDQVSDNIAGRTI
                     CALGDAAALPVKSFIKHFRDEFVFHIQNKKCLVQHDIEPKWGRSGADFHKLEKAA"
     gene            3897..>5993
                     /gene="nuoG"
                     /locus_tag="VGO02_05960"
     CDS             3897..>5993
                     /gene="nuoG"
                     /locus_tag="VGO02_05960"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEV7477267.1"
                     /translation="MLKVEIDGKIVEVPHGSTVMDATNKLGVYVPHFCYHRKLSIAAN
                     CRMCLVEVEKAPKPLPACATPVTDGMKVKTHSPAAKNAQNGVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGHSNSRYTEEKRVVFRKELGPLVAAEEMSRCIQCTRCVRFGEEI
                     GGMMELGMIGRGEHAEIVSFVGRAVDSELSGNMIDLCPVGALTSEPFRYSARTWELSR
                     RKSVSPHDGLGSNLVVQVKQNKVMRVLPLDNEAVNECWLSDKDRFSYEGLNSPDRLTR
                     PMVKKNGQWEEVDWEQALDATLRALQGWEFGVLASPHATLEELFLTSKLGPADFRLRH
                     SDFSADGKRAGIPWLGMPLTELATLDRVLVVGSFLRKDHPLIAHRLRQAAKRGTQIHM
                     LHSVDDDWLMRVKSKKIVPPSEIVAALSSFEGVLKEGKNAAVLLGNFAQQHPQAAQIH
                     AAAQALAQVTNAKVGFLGEAANSVGGYVAGLPTESLEAILARKAFVLLNVEPELDCAD
                     PQAAMAAMRRAQYVVSLSAYKTGLDYAHVLLPVAPFTETAGSFVSTEGRLQSFHATVN
                     PLGEARPAWKVLRVLGTMLGRPGFELDTVEEVRAACIGGRDIAGLLSNRISLEAKNSQ
                     EKPQGVQRIADVPIYFADPLVRRSGPLQKTRDARPPRAWMNGKLAARLGVTAKDQVLV
                     KQNAGQARLGVALDDRL"
BASE COUNT         1170 a         1878 c         1930 g         1015 t
ORIGIN      
        1 gatcttctcg gccttcaccg caacgttgag ttgaccgaac gacacggaaa atccggtcgc
       61 cgcgtcgccc agaccgccga caatcgtctg acccagcgta tcgagcctgc tgtcgtccat
      121 tccaaaaacc tcagcgctca atcgtgccgg tcgacattta tgtgccgggc tgcccgccga
      181 ccgcggaagc gctgctctac gggatcgtcc agctgcagaa caagatcaag cgcaccaaca
      241 caattgcccg atagatgaca ccgcggctgc aaaagctctt tgactcgatc aaggatctga
      301 atcccgtcga gcggctcggc gagctgaccg tcgaagtcgc cgcttctcaa tacaaatcca
      361 ttgctgaaag actgaaaagc agtgggtttg agcagctcat cgatctgtgc ggagtcgact
      421 atgcctcgta cggcgacaag ccgcgcgaag gcaaccggtt tgccgccgta attcatctgt
      481 tatctctgaa aaataactgg cgattgcggg tgcgcgcgtt ctgccccgac gattcgttcc
      541 cggtcatcga ttcgctggtc gaggtctggc cgggggtggg ctggttcgag cgcgaggcct
      601 ttgatctctt cggcatcgtc ttcgatggcc atcccgacct gcgccgcatc ctcaccgact
      661 acggcttcat cggccacccg ttccgcaagg atttcccgct ctcgggccat gtcgagatgc
      721 ggtatgaccc cgagcaacgg cgggtcatat atcaacctgt cactatcgat ccgagggagg
      781 ttaccccgcg ggtggtgcgc gaggaaaact acgccggggg cgacaggtag tggcggagat
      841 caagaactac accctcaact tcggcccgca gcacccggcg gcgcacggcg tgctgcgcct
      901 ggtgctcgag ctcgacggcg aggtgatcca gcgcgcagac ccgcatatcg gcttgctgca
      961 ccgggctacc gagaagctcg cggagcacaa gacgttcatc cagtcgatcc cctacatgga
     1021 ccggctcgac tacgtctcga tgatgtgcaa cgagcatgcc tacgtcctgg ccatcgagaa
     1081 actcctgggc atcacgccgc cgctgagagc gcagtacatc cgcacgatgt tcgacgaggt
     1141 gacgcggatc ctgaaccacc tgctgtggct cggcgcgcac ggcctggaca tcggcgcgat
     1201 gaccatcttc ctctactgct tccgcgagcg cgaggacctg atggacgcct acgaggcggt
     1261 gtcaggggcg cgcatgcacg ccgcctacta ccgcccgggc ggggtgtacc gcgacctgcc
     1321 cgattcgatg ccgcaatatg aagtttccaa aataaagaat tccgctgaaa ttcagaaact
     1381 gaatcaaaat cgccagggtt cgctgctcga cttcctggag gatttcacca accgtttccc
     1441 gaagcacgtc gacgagtacg agacgctgct caccgagaac cgcatctgga agcagcgcac
     1501 ggtgggcatc ggcgtggtgt cgccggagcg cgcgctggcg ctgggcttca ccggcccgat
     1561 gctgcgcggc tcgggcatcg agtgggacct gaggaagaag cagccctacg aggtctacga
     1621 caaggtcgac ttcgacatcc cggtgggcgc gaacggcgac tgctacgacc gctacctggt
     1681 gcgagtcgag gagatgcgcc agtccaaccg catcgtgaag cagtgcatcg actggctgcg
     1741 caagaatccc ggcccggtga tggtcgacaa ccataaagtg gcgccgcctt cgcgcgtcga
     1801 catgaaggcc aacatggaag agctgatcca ccacttcaag ctcttcaccg agggcatgca
     1861 cgtgcccgaa ggcgagtgct acgccgcggt cgaggcgccc aagggcgagt tcggcatcta
     1921 cctgatctcc gacggcgcca acaagcccta ccgcctgaag atccgagcgc ccggttttgc
     1981 ccatttgtcc gcgctcgacg agatggcgcg cggccacatg atcgccgacg cggtagccat
     2041 catcggcacg caggacatcg tcttcggcga ggtcgatcgg tgatgttgac gactgattct
     2101 ttgaaacgaa ttgacaggga aattgcgaaa tacccgcccg agcagaagaa gtcggcggtg
     2161 atgagcgcgc tggtcatcgc ccaggacgag aagggctggc tgtcgaacga cctgatggac
     2221 tatgtcgcgc agtacctcgg catggcgccg gtggcggtgt acgaggtggc gtcgttctac
     2281 accatgtaca acctcaagcc gaccggccgc cacaagctca ccatctgcac ctgcctgccc
     2341 tgcggcctgc agggctcgct cgccgccgcc gaccacctgc gcgaaaagct cggcatcggt
     2401 ttcaacgaga ccacgtcaga cggacgcttc acgctgaaag aaggcgagtg catgggcgcc
     2461 tgcgcgatgg cgccggtggt gctcgtcaac aacaagaaaa tgcacgatta catgtcgagc
     2521 gagaagctcg accagctcct ggacagcctg aaatgaagct cgactacggc cccgatgcga
     2581 tcctgatgaa ggggctggac ggcgccaact ggcgccttgc cgactatgag aagcgcgatg
     2641 gctatgccgc gctgaaaaaa atcatcagtg aaaagatcaa gcccgaagac atcatcgccg
     2701 acctgaagaa gtccgcgctg cgcgggcgcg gcggcgccgg cttcccgacc ggcctgaagt
     2761 ggtcgttcat gccgcgcgcc ttccccggcg cgaagtacgt ggtgtgcaac tccgacgaag
     2821 gcgagccggg cacctgcaag gaccgcgacc tgctgcgcta caacccgcac tcggtgatcg
     2881 aagggatgat catcgccggc tacgccatgc aggcgagcgc cggctacaac tacatccacg
     2941 gcgagatctg ggaaacctac gagctgtacg aggaggcgct gaaggaagcc tatgccgcgg
     3001 gctacctcgg caagaacatc ctgggctcgg agttcagcta cgagatgtac gcgcacccgg
     3061 gctacggcgc ctacatctgc ggcgaggaaa ccgcgctgct cgagtcgctc gaaggcaaga
     3121 aaggcatgcc gcgcttcaag ccgccgttcc cggcaagcta cggcctctac ggcaagccga
     3181 ccaccatcaa caacaccgag accttcgccg cggtaccctg ggtgatccgc aacggcggcg
     3241 aggctttcct ggcgctcggc cggccgaaca atggcggcac caagctgttt accgtgagcg
     3301 ggcatgtgaa ccgccccggc aactacgagg tgaagctggg cacgccgttc gccaagctgc
     3361 tcgagatggc cggtggcgtg cgccgcggcc gcaagctcaa ggctgttatc cccggcggtt
     3421 cgtcgatgcc ggtgctgacc gccgaggtca tgatgaagac cgacatggac tacgactcga
     3481 tcgcgaaggc cggctcggtg ctgggctcgg ggggtgtgat cgtgatggac gactcgacct
     3541 gcatggtgcg cgcgctcgag cggctgtcct acttctactt cgaggagtcg tgcggccagt
     3601 gcacgccctg ccgcgaaggc accggctggc tctaccgcgt ggtgcaccgc atcgaggagg
     3661 gcctgggccg catggaagac ctcgcgctgc tcgaccaggt ctccgacaac atcgccggcc
     3721 gcaccatctg cgcgctgggc gacgccgcgg cgttgccggt gaagagcttc atcaagcact
     3781 tccgggatga atttgttttt catattcaaa ataaaaaatg tctcgtgcag cacgacatcg
     3841 agcccaagtg gggaagatcc ggggcggatt tccacaaact tgaaaaggcg gcataagtgc
     3901 tgaaggtcga aatcgacggc aagatagtgg aagtgccgca cggttcgacc gtgatggacg
     3961 ccaccaacaa gctcggcgtc tacgtgccgc atttctgcta ccaccgcaag ctgtcgatcg
     4021 ccgccaactg ccgcatgtgc ctggtggagg tggagaaggc gcccaagccg ctgccggcct
     4081 gcgccacccc ggtcaccgac ggcatgaagg tgaagaccca ttcgccggcg gcgaagaacg
     4141 cgcagaacgg cgtcatggag ttcctgctga tcaaccaccc gctcgattgc ccgatctgcg
     4201 accagggcgg cgagtgccag ctgcaggacc tcgccgtcgg ctacggccac agcaattcgc
     4261 gctacaccga agagaagcgg gtcgtcttca ggaaggagct cggccccctg gtggcggcgg
     4321 aggagatgag ccgctgcatc cagtgcacgc gctgcgtgcg cttcggcgag gagatcggcg
     4381 gcatgatgga gctcggcatg atcgggcgcg gcgagcacgc cgagatcgtg tccttcgtcg
     4441 gcagagcagt cgattcggag ctctccggca acatgatcga cctgtgcccg gtgggggccc
     4501 tgacttccga gcctttccgc tatagcgcga ggacctggga attgtcgcgc cgcaagtcgg
     4561 tgtcgccgca cgacggcctg ggctccaacc tggtggtgca ggtcaagcag aacaaggtca
     4621 tgcgcgtgct gccgctcgac aacgaggcgg tgaacgagtg ctggctgtcc gacaaggacc
     4681 gcttctccta cgagggcctc aactcgcccg accgcctgac gcggccgatg gtgaagaaga
     4741 acggccagtg ggaggaggtc gactgggagc aggcgctcga cgccaccctg cgcgcgctgc
     4801 agggctggga gttcggtgtg ctggcttcgc cgcatgcgac gttggaggag ttatttttaa
     4861 cttctaaatt aggacctgct gatttccgcc tgcgccacag cgacttctcg gccgacggca
     4921 agcgcgcggg catcccctgg ctaggcatgc cgcttaccga gctcgcgacg ctcgaccgcg
     4981 tcctggtcgt cggctcgttc ctgcgcaagg accatccgct gatcgcgcac cgcctgcgcc
     5041 aggcggccaa gcgcggcacg cagatccaca tgctgcactc ggtggacgac gactggctga
     5101 tgcgggtgaa gagcaagaaa attgttcctc cttcggagat cgtggccgcg ctttcttctt
     5161 tcgagggagt tctcaaggaa gggaaaaatg cggccgtgct tcttggcaat ttcgcgcagc
     5221 agcacccgca ggcggcgcag atccatgccg cggcgcaggc gctggcgcag gttacgaatg
     5281 cgaaggttgg atttctcgga gaagccgcga attccgtcgg cggctatgtc gccggcctgc
     5341 cgaccgagag cctggaggcg atcctcgcga ggaaggcttt cgtgctgctc aacgtcgagc
     5401 ccgagctcga ctgcgccgac ccgcaggcgg cgatggcggc gatgcgccgg gcgcagtacg
     5461 tggtctcgct cagcgcctac aagaccgggc tcgactatgc ccatgtcctg ctgccggtcg
     5521 cgccgtttac cgagacggcc ggcagctttg tcagcacgga ggggcgcctg cagagcttcc
     5581 acgccaccgt caacccgctc ggcgaagcgc gccctgcatg gaaggtgctg cgcgtgctcg
     5641 gcaccatgct cggccggccg ggcttcgagc tcgacacggt ggaagaggtg agggccgcgt
     5701 gcatcggggg cagggacatt gcggggctgt tgtcgaatcg aatttcactt gaagcaaaga
     5761 acagccagga aaaaccgcag ggcgtgcagc gtattgccga tgtcccgatc tacttcgctg
     5821 acccgctggt gcggcgctca ggcccgctgc agaagacgcg cgatgcgcgg ccgcccagag
     5881 cgtggatgaa cgggaagctg gcggcgcgcc tgggcgtgac ggcgaaggac caggtgctgg
     5941 tgaagcagaa cgcgggtcag gctcgtctcg gggttgcgct ggatgaccgg ctg
//
LOCUS       DASYJA010000278         3044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_586677, whole genome shotgun sequence.
ACCESSION   DASYJA010000278 DASYJA010000000
VERSION     DASYJA010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586677"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VGO02_05965"
     CDS             <1..469
                     /locus_tag="VGO02_05965"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEV7477268.1"
                     /translation="SQQPTLAYGIFAAYILAHFLMIAIMLLGVRWFLKVVAVPKSILF
                     PIILVLCTVGAFALDNTMANVYVLLLFGLVGYAMVKAGLPLEPLILGVILGDQIEINL
                     VRAIMTDDNPWLFLTRPISGVLLLLSVLSVAFALWQHHRMPKKELAAEDVADF"
     gene            complement(483..558)
                     /locus_tag="VGO02_05970"
     tRNA            complement(483..558)
                     /locus_tag="VGO02_05970"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(523..525),aa:Arg,seq:cct)
     gene            647..1501
                     /locus_tag="VGO02_05975"
     CDS             647..1501
                     /locus_tag="VGO02_05975"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV7477269.1"
                     /translation="MKRLFLAVLAVALPASAQPQTWAWDDGTVPYVQTPMEIVERMMR
                     MAEVGKGDHLIDLGSGDGRIVIEAAKRGATGLGVDLDPALVKYATENAQKAGVAERTR
                     FEVRDIFETDLSKATVVAFYLLPEFNAKLLPKLLALKPGTRIVSHDGGIGDWPPDERL
                     EMRAPEKPVGVGGVSKVELWIVPADARGAWTSEIPAHGGTWKFAIKQKFQELDIAAAA
                     QGRDILVRNSRLRGTEIKMVITGLVGTRAWHHLFEGRIDGERIAGEVTVSDGNNRRTF
                     PWTATRSK"
     gene            1477..2817
                     /locus_tag="VGO02_05980"
     CDS             1477..2817
                     /locus_tag="VGO02_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV7477270.1"
                     /translation="MDRNPKQVKPKELLSVTDGVLLTAGMIIGALIFKAPSTVAGATG
                     GAGQFLFAWLLGGLVSLCGALVYAELASRHPDTGGEYMFLMRGWGRAVAFLFAWSRLT
                     VIQTGAIAAVAFVFGDYASEILRLGAHSSAIWAAIAVAALTALNVAGTLQTKLLQKVM
                     EISLITGLVVFSIAALVKGGAPAESAAPGAGFGAFGFAMIFVLLAYGGWNEAAYLAGE
                     VKDGRRNMTRILVIGVLAVTALYLLVNLAYLAVLGHGGIKASKAVAADVMRAIAGDKG
                     AVVLALIVCVSVLTTINAAIFTGARTNYALGRDFHFLRALGQWRESGSTPANALLLQG
                     VITLILVGASALTPDGFNAMVAYTSPVFWTFFLLTGLTLFVFRFRNETAGSFKVPLYP
                     IVPVAFVLTCAYMLYSSINYVRNPEYGPKFGIAVLAGLAVMAAGVPLYFFAKKK"
     gene            2814..>3044
                     /locus_tag="VGO02_05985"
     CDS             2814..>3044
                     /locus_tag="VGO02_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV7477271.1"
                     /translation="MTLTELRYIVAVARERHFGRAAEACFVSQPTLSVAVKKLEEELG
                     VTLFERGPEVTVTPGGSRIVEQAQRVLEEASRI"
BASE COUNT          466 a          995 c         1037 g          546 t
ORIGIN      
        1 gtcgcagcag ccgacgctcg cctacggcat cttcgctgcg tacatcctgg cgcacttcct
       61 gatgatcgcc atcatgctgc tcggcgtgcg ctggttcctg aaagtcgtag ccgtgccgaa
      121 gtcgatcctg ttcccgatca tcctggtgct gtgcaccgtc ggcgccttcg cgctcgacaa
      181 caccatggcc aatgtctatg tgctgctgct cttcgggctg gtcggctacg ccatggtcaa
      241 ggccgggctg ccgctcgagc cgcttatcct cggtgtgatc ctcggcgacc agatcgagat
      301 caacctggtg cgcgccatca tgaccgacga caacccgtgg ctgttcctga cgcggccgat
      361 ttcaggcgtc ctcctgctcc tgtcggttct gtcagtggcc tttgcgctgt ggcagcacca
      421 ccgtatgccg aagaaggaac tggcggccga ggatgtcgcc gatttctaga cagacggtgc
      481 gttggcgccc aagagacgaa tcgaacgtcc gacctgcccc ttaggagggg gctgctctat
      541 ccactgagct acctgggccc ggccgcgatt ttacgcgagg aatttgcgcc tttcccggta
      601 gacttagccc ttctgtatgg cggatgggta atcgatagtg ctagtgatga agcggctgtt
      661 cctcgcagtc ctcgcagtgg ctctcccggc aagcgcgcaa ccccagacct gggcctggga
      721 tgacggaacc gttccctatg tgcagacgcc gatggagatc gtcgagcgga tgatgcgcat
      781 ggcggaggtg gggaagggcg accacctgat cgacctgggc tcgggcgacg ggcgcatcgt
      841 gatcgaggcg gcgaagcgcg gcgcgaccgg gctcggtgtc gatctggatc cggccctggt
      901 gaaatacgcg accgagaacg cgcagaaggc gggcgtggcg gagcggaccc gcttcgaggt
      961 gcgcgatatc ttcgagacgg acctctccaa ggcgacggtc gtcgctttct atctgctgcc
     1021 ggaattcaac gccaagctgc tgcccaagct gctcgcgctc aagcccggca cgcgcatcgt
     1081 gtcgcacgac ggcggcatcg gcgactggcc gcccgacgag cgcctggaga tgcgcgcgcc
     1141 ggagaagccg gtcggcgtcg gcggcgtgtc gaaggtcgag ctgtggatcg tgccggccga
     1201 cgcacgcggc gcttggacga gcgagatacc cgcgcacggc gggacgtgga aatttgcgat
     1261 caagcagaag ttccaggagc tggacatcgc cgccgccgcg cagggccgcg acatcctggt
     1321 gcgcaacagc cgcctgcgcg gcaccgagat caagatggtg atcaccggcc tggtcggcac
     1381 gcgcgcctgg caccacctgt tcgaaggccg catcgacggc gagcgcatcg ccggcgaggt
     1441 cacggtttcc gatggcaata acagaaggac gttcccgtgg accgcaaccc gaagcaagtg
     1501 aaacccaagg aactgctgtc cgtcaccgac ggggtgctgc tcaccgccgg gatgatcatc
     1561 ggcgcgctga tcttcaaggc gccctcgacg gtcgccggcg cgaccggcgg cgccggccaa
     1621 ttcctcttcg cctggctgct cggcgggctg gtctcgctgt gcggcgcgct ggtctatgcc
     1681 gagctcgcct cgcgccatcc cgacaccggc ggcgagtaca tgttcctgat gaggggctgg
     1741 gggcgcgcgg tcgctttcct cttcgcctgg tcgcggctga cggtgatcca gaccggcgcc
     1801 atcgccgcgg tggcctttgt cttcggcgac tacgcttccg agatcttgcg cctcggcgcg
     1861 cacagctcgg cgatctgggc ggcgatcgcg gtcgcggcgc tcaccgcgct caacgtcgcc
     1921 ggcacgctgc aaacgaagct gctgcagaag gtgatggaga tcagcctgat caccgggctg
     1981 gtggtgttct cgatcgccgc gctggtgaag ggcggggcgc cggccgagag cgccgcgccc
     2041 ggcgccggct tcggcgcctt cggcttcgcc atgatcttcg tgctgctcgc ctacggcggc
     2101 tggaacgagg ccgcctacct ggcgggcgag gtgaaggacg ggcggcgcaa catgacccgc
     2161 atcctggtga tcggggtgct cgccgtgacc gcgctctacc tgctggtcaa cctggcctat
     2221 ctcgcggtgc tcggccacgg cggcatcaag gcgtcgaagg cggtggcggc ggacgtgatg
     2281 cgcgccatcg ccggcgacaa gggcgcggtg gtgctcgcgc tcatcgtctg cgtctcggtc
     2341 ctcaccacca tcaacgccgc gatcttcacc ggcgcgcgca ccaactacgc gctgggccgg
     2401 gacttccatt tcctgagggc tctcgggcaa tggcgcgagt cgggcagcac gccggccaac
     2461 gcgcttctcc tgcaaggcgt gatcacgctg atcctggtcg gcgcgagcgc gctgaccccg
     2521 gacgggttca atgcgatggt ggcgtacacg tcgcccgtct tctggacgtt ctttctgctg
     2581 accggcctga cattgttcgt cttccgcttc cgcaacgaga cagccggaag cttcaaggtg
     2641 ccgctctacc ccatcgtgcc ggtcgccttc gtcctgacgt gcgcgtacat gctgtactcg
     2701 agcatcaact acgtgcgcaa ccccgagtac ggcccgaagt tcggcattgc cgtgctggcg
     2761 gggctagcgg tgatggcggc gggagtaccg ttgtacttct tcgcgaagaa gaaatgaccc
     2821 tcaccgagct tcgctacatc gtcgcggtgg cgcgcgagcg ccacttcggc cgcgcggcgg
     2881 aggcctgctt cgtatcgcag ccgaccttgt cggtggcggt gaagaagctg gaggaggagc
     2941 tgggcgtcac gctcttcgag cgcggcccgg aggtgacggt cactccgggc ggcagccgga
     3001 tcgtcgagca ggcgcagcgc gtgctcgagg aagcctcgcg catc
//
LOCUS       DASYJA010000279         3552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2765170, whole genome shotgun sequence.
ACCESSION   DASYJA010000279 DASYJA010000000
VERSION     DASYJA010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2765170"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VGO02_05990"
     CDS             <1..273
                     /locus_tag="VGO02_05990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7477272.1"
                     /translation="FEDSRAVGTGRLLPDGHIGRMAVLKEFRGKGAGGALLAKLMEAA
                     KSRGDREVVLSAQVHAVPFYQAYGFELFGEIYEDAGIPHRDMRRDL"
     gene            complement(270..923)
                     /locus_tag="VGO02_05995"
     CDS             complement(270..923)
                     /locus_tag="VGO02_05995"
                     /inference="COORDINATES: protein motif:HMM:NF017551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HEV7477273.1"
                     /translation="MCLIVLAWRKLADYPLVVAANRDEFHGRPAAPAAFWPDQPAILA
                     GRDLEAKGTWMGVARNGRFAAVTNYRGATEPRAAESRGALVSRFLAGNEEPETYLKSI
                     QPGRYSGFNLLVADRELWSMSNRDGPPRRLEPGLYGLGNTLLDAAEVDRPKERFAAVL
                     GPAVEPLFTLLAESKIVNERYGTRCSTVLIRGKNRVRYAERSFSPDGTDQETLHYEF"
     gene            complement(914..1168)
                     /locus_tag="VGO02_06000"
     CDS             complement(914..1168)
                     /locus_tag="VGO02_06000"
                     /inference="COORDINATES: protein motif:HMM:NF027614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4936 family protein"
                     /protein_id="HEV7477274.1"
                     /translation="MNYYIYYKVEPELLALLRSAVEELFEKIEKQTGIRGRWMHRRDD
                     PLTYMEVYEGVKDEKAFEALLEREGAKLGVPRKVERFVCA"
     gene            complement(1165..1908)
                     /locus_tag="VGO02_06005"
     CDS             complement(1165..1908)
                     /locus_tag="VGO02_06005"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEV7477275.1"
                     /translation="MERARLTRYGLLSVTGADARAFLHAQLTQDIENLPPERAALAGW
                     CSAKGRLLASFLVIPANDGFLLQLARDLAPAVAKRLSMFVLRSKVKIADVSDAWQQMG
                     IWGEDFAGEIFSIKRENNLLQVKVGERRALLLGPEPEMRFRTATGAEEAWTLEEIRAG
                     RPFISAATQDQFVPQMVNLETIGGVDFQKGCYPGQEIVARAQYRGQVKRRMVRLQAPA
                     GVQLKPGEAFNGGVVVDAAGGELLAVVPI"
     gene            1941..2936
                     /gene="mltG"
                     /locus_tag="VGO02_06010"
     CDS             1941..2936
                     /gene="mltG"
                     /locus_tag="VGO02_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEV7477276.1"
                     /translation="MKAFFKTLLALVLLGVAAIAAYGIWYLRTPLTMQQAPLEVEIPR
                     GASLPSVVNSLENAGLHVHRREFVLLVRAFGRSRDLKAGNYLLAKAPTPIELLEKLTR
                     GDVMQAEVRLIEGWTFTQFRNALDSHQDLKHDTRGLDDAEVLKRLQAAEPHPEGLFFP
                     DTYLFAKGSSDIAVLRSAYRAMQRHLQAEWDARQQNVPYKSPYEALIMASIIEKETGA
                     PAERDQIGGVLVNRLRIGMRLQVDPTIIYGLGESFDGNLKKKHLLQDAPYNTYTRAGL
                     PPTPIAMPGLASLRAAMRPTKTDAMYYVSRGDGSSQFSRNLAEHNRAVTKYQLKR"
     gene            2933..3547
                     /gene="tmk"
                     /locus_tag="VGO02_06015"
     CDS             2933..3547
                     /gene="tmk"
                     /locus_tag="VGO02_06015"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV7477277.1"
                     /translation="MSPRGKLITLEGVDGAGKSTHVQFIADTIGAGSRHVVVTREPGG
                     TDFAEQLRKVVLTQTLPPVVVTLIVFAGRGDHVSKVIEPALAAGSSVVCDRFTDATIA
                     YQGAGQGVARELIDELAREVHPSLVPDRTLIFDVPYEVASQRLAASGKKLDRFEREER
                     AFFDRVRNAYLAIAKAEPKRVRLIDASSDPAVIRQAISRELAGL"
BASE COUNT          592 a         1224 c         1159 g          577 t
ORIGIN      
        1 ttcgaggatt cgcgtgcagt gggcaccggg cggctgctgc ccgacggcca catcggccgc
       61 atggcggtgc tgaaggagtt ccgcggcaag ggcgcaggcg gcgccttgct ggccaagctg
      121 atggaggcgg ccaagtcgcg cggcgatcgc gaagtcgtgc tctcggcgca ggtgcacgcg
      181 gtgccgttct accaggcata cggcttcgag ctgttcggcg agatctacga ggacgccggc
      241 atcccgcacc gggacatgcg gcgcgatctc tagaactcgt agtgcagggt ttcctggtcg
      301 gtgccgtcgg gcgagaaact ccgctccgca taacggactc tgttttttcc tcttatcaga
      361 actgtggagc agcgtgttcc ataccgctcg ttcacgatct tcgattcagc gagcagggtg
      421 aatagcggct cgactgcggg cccgaggacc gcggcgaagc gctccttggg ccggtcgact
      481 tccgccgcat cgagcagggt gttgcccagg ccgtagaggc cgggctcgag gcgccgcggc
      541 gggccgtcgc ggttcgacat cgaccacagc tccctgtccg ctaccagcaa attgaagccg
      601 ctgtaccggc cgggctggat gcttttcaaa tacgtttccg gttcttcatt tcctgccaga
      661 aatctgctaa ccagagcgcc gcgagattcc gcggctcgcg gctcggtcgc gccgcgatag
      721 ttcgtcaccg cggcgaagcg gccgttgcgc gccacgccca tccaggtgcc cttcgcctcc
      781 agatcgcggc cggcgaggat cgccggctgg tcgggccaga acgcggcggg ggcggcgggg
      841 cggccatgga actcgtcgcg gttggccgcg accaccagcg ggtaatcggc aagcttgcgc
      901 caggcgagga cgatcaggca catacgaagc gctcgacctt gcgcggcacg ccgagcttcg
      961 cgccctcgcg ctcgagcagc gcttcaaagg ccttctcgtc cttcacgcct tcatagactt
     1021 ccatgtacgt gaggggatcg tcgcggcggt gcatccagcg accgcggatg ccggtttgct
     1081 tttcgatttt ctcgaacagc tcttccacgg cagagcgcaa gagcgccaac agctcaggct
     1141 cgaccttgta gtagatgtag taattcaaat cgggaccacg gcgaggagct cgccgccggc
     1201 ggcatccacc accacgccgc cgttgaacgc ctcgccgggc ttgagctgca ccccggcggg
     1261 cgcctgcagg cgcaccatgc ggcgcttcac ctgcccgcga tactgcgcgc gcgccacgat
     1321 ctcctgcccg ggatagcagc ccttctggaa gtcgacgccg ccgatcgtct cgaggttcac
     1381 catctgcggc acgaactggt cctgcgtcgc cgcgctgatg aacggccgcc cggcgcggat
     1441 ctcctccagc gtccaggctt cttcggcacc ggtcgcggtg cggaaacgca tctcgggctc
     1501 gggcccgagg agcagggcgc gccgctcgcc gaccttgacc tgcagaagat tgttctcccg
     1561 cttgatggag aaaatctcgc cggcgaagtc ctcgccccag atgcccatct gctgccaggc
     1621 gtcgctgacg tcggcgatct tcaccttgga gcgcaggacg aacatggaca ggcgcttggc
     1681 caccgcgggc gccaggtcgc gcgccagctg caggaggaag ccgtcgttcg ccgggatgac
     1741 gagaaagctc gccagcagcc gtcccttggc cgagcaccag ccggcgagcg ccgcgcgttc
     1801 cggcggcagg ttctcgatgt cctgggtgag ctgtgcgtga agaaatgcgc gcgcgtcggc
     1861 gccggtgacc gagagcaaac cgtagcgggt gaggcgcgcg cgctccatcg tagaatgcac
     1921 attttaccgt cggcgcaaac ctgaaagcct tctttaaaac cctgctcgcg ctggtgcttc
     1981 tgggcgtggc cgcgatcgcg gcctacggca tctggtactt gcgcaccccg ctgacgatgc
     2041 agcaggcgcc gctcgaggtc gaaatcccgc gcggcgccag tctgccttca gtagtaaaca
     2101 gtctggaaaa cgccggcctg cacgtccatc gccgcgagtt cgtgctgctg gtgcgcgcct
     2161 tcggccgcag ccgcgacctc aaggccggca actacctcct cgccaaggcg ccgacgccga
     2221 tcgagctgct ggagaagctc acgcgcggcg atgtcatgca ggccgaggtg cggctgatcg
     2281 agggctggac cttcacgcag ttccgcaacg ccctcgactc gcaccaggac ctgaagcacg
     2341 acacccgggg cctggacgac gccgaggtgc tgaagcggct gcaggcagcc gagccgcacc
     2401 cggagggcct gttctttccc gacacctatc tcttcgccaa aggctcgagc gacatagccg
     2461 tgctgcgcag cgcctaccgc gccatgcagc gccacctgca ggccgagtgg gacgcgcgcc
     2521 agcagaacgt gccgtacaag tcgccctacg aggcgctgat catggcctcg atcatcgaga
     2581 aggagaccgg cgcgcccgcc gagcgcgacc agatcggcgg cgtgctggtg aaccgcctgc
     2641 gcatcggcat gcggctgcag gtcgatccga ccatcatcta tggcctgggc gagagcttcg
     2701 acggcaacct gaagaagaag cacctgctgc aggacgcgcc gtacaacacc tacacgcgcg
     2761 ccggcctgcc gccgacgccg atcgcgatgc cgggcctcgc gtcgctgcgc gccgccatgc
     2821 gcccgacgaa aaccgacgcc atgtattacg tctcgcgggg cgacggctcg agccagttct
     2881 cgcgcaacct cgcggagcac aaccgcgccg tgacaaaata tcagctcaaa cgatgagccc
     2941 gcgcggcaag ctgatcactc tggaaggcgt tgacggcgcg ggaaagtcca cccatgtgca
     3001 gttcatcgcc gatacgatcg gcgcaggttc ccgccatgtc gtggtcacgc gcgagcccgg
     3061 cggcaccgat ttcgccgagc aactgcgcaa agtcgtcctc acgcagacgc tgccgcccgt
     3121 cgttgtgacg ctcatcgtct tcgccgggcg cggcgaccac gtctcgaaag tgatcgagcc
     3181 cgccctcgcc gccggcagct cggtggtctg cgaccgcttt accgatgcga cgatcgcgta
     3241 ccagggagcg gggcagggcg tcgcccgcga gctgatcgac gagctggcgc gcgaggtcca
     3301 tcccagcctc gtcccggacc gcaccctgat cttcgacgtc ccgtacgagg tcgcgagcca
     3361 gcgcctcgcc gcttccggca agaagctcga ccgattcgag cgggaagagc gcgctttctt
     3421 cgaccgcgtg cgcaacgcct acctggcgat cgccaaggcg gagccgaagc gcgtgcgcct
     3481 catcgacgct tcctctgacc cggcggtgat ccgccaggcc atttcgcgcg agctggcggg
     3541 actctgatgc at
//
LOCUS       DASYJA010000280         3015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_496864, whole genome shotgun sequence.
ACCESSION   DASYJA010000280 DASYJA010000000
VERSION     DASYJA010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496864"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VGO02_06020"
     CDS             complement(<1..656)
                     /locus_tag="VGO02_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HEV7477278.1"
                     /translation="MKSTFTQRGEKVRLDDIPCEPPKGMTREKAAERLLALGKEMFEL
                     QDTMWGAKGNSVLVVLQGRDSAGKDGAIKHVAGFLNPRGVHVASFGVPTEEERQHDFL
                     WRVHRHAPRLGEFAIFNRSHYEDVLVTRVRGLMPKKLWRERYGHIADFEELLAEHGTI
                     VLKYFLHITKAEQKKRLLEREKAPETAWKLNPNDWVERDYWDDYTEAYEDAIAKTAAP
                     "
     gene            complement(657..1187)
                     /gene="def"
                     /locus_tag="VGO02_06025"
     CDS             complement(657..1187)
                     /gene="def"
                     /locus_tag="VGO02_06025"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV7477279.1"
                     /translation="MIRDVLRMGDPRLWQVSLPVKTFGTELQELLQDMRDTMAHLNGA
                     GLAAPQIGVGLRVVIFGVTANPRYPDVEEVPQTVLINPELTPLTAEEEEAWEGCLSVP
                     GMRGVVPRWQRVRYQGFDEKGEPFSREVAGFHARVVQHECDHLDGVLYPMKIRDFTRF
                     GFIEELFPGEDLPAEE"
     gene            complement(1184..1927)
                     /locus_tag="VGO02_06030"
     CDS             complement(1184..1927)
                     /locus_tag="VGO02_06030"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HEV7477280.1"
                     /translation="MSLLAIIGGSGLSQLGSLEVTQRKVSRTPYGEPSGALTFGRIAD
                     CDVVFLARHGYGHTIAPHEVNYRANLWALKDAGANEVVSVASVGGIRNDTWPGTLVLP
                     HQIIDYTWGRGATFFEGAGVPVNHIDFTEPYSRGLRERLLEAAGRCGETLADGGVYAA
                     TQGPRLESAAEINRLERDGADLVGMTGMPEASLAREISLQYATIAVVANYAAGRGDSA
                     HAIPLDKIGKVLEEAMGRVRRIIGALCEK"
     gene            complement(1924..2472)
                     /locus_tag="VGO02_06035"
     CDS             complement(1924..2472)
                     /locus_tag="VGO02_06035"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEV7477281.1"
                     /translation="MPDVQKAWAFLENSEPVASAKEVEAAVQRLAAEIGERLADAYPL
                     VLAVMGGAVVFAGQILPRLRFPLDFDYIHASRYGPATRGAQIEWRVTPPSAVKGRAVL
                     VLDDILDGGVTMAAIRTRLIELGAKSFHCAVLVEKKLKVEKPIKADFVGLQIADRFVF
                     GCGLDAKGFWRNLPEIRAMKEG"
     gene            complement(2482..>3015)
                     /locus_tag="VGO02_06040"
     CDS             complement(2482..>3015)
                     /locus_tag="VGO02_06040"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEV7477282.1"
                     /translation="LGHAVLCAQPVVGNDPFAVLLADDLMDSNPPAMARMAEIFHKEG
                     ACLLGVEEVPRDQTQSYGIVTIDRMVDDIARLHSIVEKPKPAAAPSNLAVIGRYVLTP
                     RIFDLLAAVTPGAGGEIQLTDAISALLAHEQVFAVRLPGRRFDCGSKLGYLQAMVEFG
                     LRHPETGEAFARYLKSR"
BASE COUNT          454 a         1058 c         1010 g          493 t
ORIGIN      
        1 tgcggcgccg cggtcttcgc gatcgcgtcc tcataggcct cggtgtagtc gtcccagtag
       61 tcgcgctcta cccaatcgtt cggattgagc ttccaggcgg tctcgggggc cttctcgcgc
      121 tcgagcagcc gcttcttctg ctctgccttc gtgatgtgca ggaagtactt gagcacgatg
      181 gtgccgtgct cggcgagcag ctcctcgaaa tcggcgatgt ggccgtagcg ctcccgccag
      241 agcttcttcg gcatcaggcc gcgcacccgc gtaaccagca cgtcctcgta gtgcgagcgg
      301 ttgaagatgg cgaactcgcc gagtcgcggc gcgtggcggt gcacccgcca caggaagtcg
      361 tgctggcgct cctcctcggt gggaacgccg aaggaagcga cgtgcacccc gcgcggattg
      421 agaaagccgg cgacgtgctt gatggcgccg tccttgcccg cgctgtcgcg cccctgcagg
      481 accaccagca cgctgttgcc cttggcgccc cacatcgtgt cctgcagctc gaacatctcc
      541 ttgcctagcg ccagcaagcg ctcggccgcc ttttccctgg tcatgccctt cggcggctcg
      601 cacgggatgt cgtcgaggcg gaccttctcg ccgcgctgcg tgaacgtgct cttcaactac
      661 tcctccgccg gcaggtcctc gccggggaac agctcttcga tgaagccgaa gcgggtgaag
      721 tcgcggatct tcatcggata gaggacgccg tcgaggtggt cgcactcgtg ctgcacgaca
      781 cgcgcatgga agcccgccac ctcacggctg aagggctcgc ccttttcgtc gaagccctgg
      841 tagcgcaccc gctgccagcg cggcaccacg ccgcgcatgc ccggcacgga aaggcagcct
      901 tcccaggcct cctcttcctc ggcggtaagc ggcgtgagct cggggttgat gagcaccgtc
      961 tgcggcacct cctcgacgtc cgggtagcgc gggttcgccg tcacgccgaa aatgaccacg
     1021 cgcaggccga cgccgatctg cggcgcggcc aggccggcgc cgttgaggtg cgccatggtg
     1081 tcgcgcatgt cctgcaacag ctcctgcagt tccgttccaa acgtttttac tggcagagaa
     1141 acctgccaca gcctcggatc ccccatgcgc aggacgtccc ggatcatttc tcgcagagcg
     1201 ctccgatgat gcggcgcacg cgccccatcg cttcctcgag caccttgccg atcttgtcga
     1261 gcgggatggc gtgcgcgctg tcgccgcggc cggcggcgta gttggcgacc acggcgatgg
     1321 tggcgtactg caggctgatc tcgcgcgcaa gcgacgcctc gggcatgccg gtcatgccga
     1381 cgaggtcggc gccatcgcgc tccagccggt tgatctccgc ggcggattcg aggcgcggcc
     1441 cctgcgttgc ggcgtagacc ccaccgtcgg cgagcgtctc gccgcagcgc ccggccgcct
     1501 cgaggagccg ctcgcgcagc ccgcgcgagt acggctcggt gaaatcgatg tggttcaccg
     1561 gcacgcccgc gccctcgaag aaggtcgcgc cgcggcccca ggtgtagtcg atgatctggt
     1621 gcggcagcac cagcgtgccc ggccaggtgt cgttgcggat gccgccgacc gaggcgaccg
     1681 agaccacctc gttggcgccg gcgtccttca gggcccacag gttggctcgg tagttgacct
     1741 cgtgcggcgc gatcgtgtgg ccgtagccgt gccgcgcgag gaacaccacg tcgcaatccg
     1801 cgatgcggcc gaaggtgagc gcgccggaag gctcgccata gggcgtgcgc gagaccttgc
     1861 gctgcgtcac ctcgaggctg ccgagctgcg acagcccgct gccgccgatg atcgcgagca
     1921 agctcacccc tctttcatgg cacggatctc cggcaggttg cgccagaaac ccttcgcgtc
     1981 caggccgcag ccgaagacga agcggtcggc gatctgcagg ccgacgaaat cggccttgat
     2041 cggtttctcc accttcaatt tcttctccac caggacggcg caatggaagg atttcgcgcc
     2101 gagctcgatc aggcgggtgc ggatcgccgc catggtgacg ccgccgtcga ggatgtcgtc
     2161 cagcaccagc acggcgcggc ccttgacggc cgacggcggc gtgacgcgcc attcgatctg
     2221 cgcgccgcgc gtcgccgggc catagcgcga ggcgtggatg tagtcgaaat cgagggggaa
     2281 gcgcagccgc ggcaggatct gcccggcgaa caccacggcg ccgcccatca ccgccagcac
     2341 caggggatag gcgtcggcca gccgctcgcc gatctcggcg gccaggcgct gcaccgccgc
     2401 ctcgacctcc ttcgcgctcg ccaccggctc cgaattctcg aggaaggccc aggccttctg
     2461 cacgtcgggc atggcccgag tctagcgtga cttcaggtag cgcgcgaaag cctcgccggt
     2521 ctccggatgg cgcaggccga actcgaccat cgcctgcagg tagccgagct tggagccgca
     2581 atcgaagcgc cgccccggca ggcgcaccgc gaacacctgc tcgtgcgcga gcagcgcgga
     2641 aatggcgtcg gtcagctgga tctcaccgcc ggcgcccggc gtcaccgcgg ccagcagatc
     2701 gaagatgcgc ggcgtcagca cgtagcgccc gatcaccgcg aggttggagg gcgcggcggc
     2761 cggcttgggc ttctcgacga tgctgtgcag gcgcgcgatg tcgtccacca tccggtcgat
     2821 ggtgacgatg ccgtagctct gcgtctggtc gcgcggcact tcctcgacgc cgagcaggca
     2881 ggcgccttcc ttgtggaaaa tctccgccat gcgcgccatg gcgggcggat tggagtccat
     2941 caggtcgtcc gccagcagca ccgcgaacgg atcgttgccg acgaccggtt gcgcgcaaag
     3001 cacggcgtgg ccgag
//
LOCUS       DASYJA010000281         3011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1585072, whole genome shotgun sequence.
ACCESSION   DASYJA010000281 DASYJA010000000
VERSION     DASYJA010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585072"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VGO02_06045"
     CDS             <1..182
                     /locus_tag="VGO02_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477283.1"
                     /translation="LGQYLLNQPNGCKTIRDMIADDIGWARGRGDGRHAQELVLTLQG
                     FVSSHAADGELPLGY"
     gene            complement(343..648)
                     /locus_tag="VGO02_06050"
     CDS             complement(343..648)
                     /locus_tag="VGO02_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009769818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477284.1"
                     /translation="MELAAGGRRPQGSLTMAGIPYVFTISHVAKLLGEDEQWLWEISI
                     NMDPEEGCLWVIGVGEDGCPAFTEYGIECLRQIIIEERALAEARKPDGDALTLRCSP"
     gene            complement(606..2261)
                     /locus_tag="VGO02_06055"
     CDS             complement(606..2261)
                     /locus_tag="VGO02_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011427389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEV7477285.1"
                     /translation="MDSPSDDIERLKAELAAALMARASETERAARVEAEFALARAKMA
                     EDAATIARQNLEIAKLRRQIYGPRSERTARLLDQMELELEDLEAAATEDELAAEQTAA
                     RTTTVAAFTRKRPARQPFPEHLLRERVVVPGLMACTCCGGTRLRKLGETITETLEVIP
                     RQWKVIQHVREKMTCRDCERISEPPAPFHVTPRGWAGPNLLAMILFEKFGQHQPLNRQ
                     SERYAREGVPLSLSTLADQVGSGCAALEPLLRRLEAHVFAAERLHGDDTTVPVLAKGR
                     TVTGRCWVYVRDDRPFGGRAPPAAMFYYSRDRTGAHVEEHLARWSGLLQADAYSGYNK
                     LYVAGRQPGPILEAACWAHARRPFFALADIEASARRRAEGKAPAPISPLALEAVRRID
                     ALFEIERSINGRSAEERLAVRRELSAPLVATLEAWMRQERPKLPRGNDVAKAMDYMLK
                     RWTAFTRFLEDGRICLSNNAAERALRGIALGRKSWLFAGSDRGGARAAAMYRLIVTSK
                     MNEIDPQAWLADVLTRIADHPVHRLDELLPWNWRPAGADLRAA"
     gene            complement(2310..2657)
                     /gene="tnpB"
                     /locus_tag="VGO02_06060"
     CDS             complement(2310..2657)
                     /gene="tnpB"
                     /locus_tag="VGO02_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183759.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV7477286.1"
                     /translation="MIPVPSGMRVWLSVGRTDMRRGMNGLALQVQETLGRDPFAGDLF
                     VFRGARSDLIKVLWHDGLGLSLYAKRLERGRFVWPSTSGGSVAISAAQLAYMLDGIDW
                     RNPVHTFRPERAG"
     gene            complement(2870..3011)
                     /locus_tag="VGO02_06065"
                     /pseudo
     CDS             complement(2870..3011)
                     /locus_tag="VGO02_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          511 a         1023 c          973 g          504 t
ORIGIN      
        1 ctttaggcca atatctgctg aaccagccta atggctgcaa gaccatacga gacatgatcg
       61 ccgacgatat tggctgggcg cgtgggcgtg gcgacggcag gcatgcacaa gagctagttc
      121 tgaccctcca ggggttcgtc agctcgcatg ccgcggacgg tgaactgccc ctaggctatt
      181 gacttgtcct cggtgcaggt gcccgtggca taggtttgtg gcgggtcctt acctttcgag
      241 cacatagcga cctggcgcat cagccaaggg cggaaactcg ttgcagcccg gctgccgagc
      301 gccgaccggt tcaccgccgt agcgcgcaag ccacgtgtaa tcctacggtg agcaccgcag
      361 ggtcaatgca tccccatcag gtttgcgcgc ttcagcgagc gctcgctcct cgatgatgat
      421 ctgtctgagg cattcgatgc cgtattcggt gaatgccggg cagccatcct cgccgacacc
      481 gatgacccag agacagccct cctcggggtc catgttgatg gagatctccc acagccactg
      541 ctcgtcctcg ccgagcaact ttgcgacgtg ggagatcgtg aagacgtagg ggatgccggc
      601 cattgtcagg ctgccctgag gtcggcgccc gccggccgcc agttccacgg cagcagctcg
      661 tcgagccgat ggacaggatg gtcggcgatg cgggtgagca cgtcggcgag ccaagcctgt
      721 gggtcgatct cgttcatttt ggacgtgacg atcaggcggt acattgcggc ggcgcgcgca
      781 cccccccgat cggagccggc gaacagccac gactttcttc cgagcgcaat accgcgcagt
      841 gcgcgttctg cggcgttgtt cgacaggcag atgcggccgt cctccaggaa gcgggtgaag
      901 gcggtccacc gcttcagcat gtagtccatg gctttggcga catcgttgcc gcgcggcagc
      961 ttcggtcgct cctgccgcat ccaggcttcg agcgtggcga ccaaaggtgc gctcagctcc
     1021 cggcggaccg caagccgttc ctccgcgctt cgcccattga tgctgcgctc gatctcgaac
     1081 agggcgtcaa tgcgccggac ggcctccaac gccaatggcg agatcggcgc gggcgctttg
     1141 ccctcggccc tgcgccgcgc actggcctcg atatcggcga gcgcgaagaa tggtcggcgc
     1201 gcatgcgccc agcaggccgc ctccaggatc gggcccggct gacgaccggc cacatacagc
     1261 ttgttgtagc cagagtaggc gtcggcctgc agcagaccgg accatcgggc cagatgttcc
     1321 tcgacgtgcg caccagtgcg atcgcgggag tagtagaaca tggcggccgg cggcgctctt
     1381 ccgccgaagg gccgatcgtc gcgcacgtaa acccagcagc gtccagtgac ggtccggcct
     1441 ttggcgagca cgggtaccgt tgtgtcgtcg ccgtgcagcc gctcggcggc gaacacatgg
     1501 gcttcgaggc gccggagcag cggctccagc gccgcgcagc cggaccccac ctgatcggcg
     1561 agcgtcgaca ggctcaaggg cacgccttcc cgcgcatagc gctcggactg ccggttcaat
     1621 ggctgatgct ggccgaactt ctcgaacaga atcatggcga gcaggttggg gccagcccat
     1681 ccgcgtggcg tgacgtggaa cggcgccggc ggctcgctga tccgctcgca gtcccggcac
     1741 gtcatcttct cgcgcacgtg ctggatcacc ttccactggc gcgggattac ctccagcgtc
     1801 tcggtgatgg tctcgccgag cttcctcagg cgcgtaccac cacaacaggt acacgccatc
     1861 agtcccggca ccaccacccg ctcgcgcagc agatgctcgg ggaacggctg ccgcgccggg
     1921 cgtttgcgcg tgaaggcagc caccgtcgtc gtcctggccg ccgtctgctc ggcggcgagc
     1981 tcgtcttcgg tggcggccgc ttccaggtct tcgagctcaa gctccatctg atcgaggagg
     2041 cgcgcggtgc gctccgagcg gggcccgtag atctgacgcc tcagcttcgc gatctcgagg
     2101 ttctggcggg cgatggtcgc cgcgtcctcg gccatcttgg cgcgagcgag tgcaaactcg
     2161 gcttccaccc tggcagcgcg ctccgtctcc gatgcgcgcg ccatgagtgc ggcggccagt
     2221 tcggccttca acctctcgat gtcgtcggat ggcgaatcca tgagacgaag gaatcacaga
     2281 ctgtcgtcgc ccgctcgcag aaagcgcagc tatccagcac gttccggccg gaatgtgtga
     2341 accgggttgc gccaatcgat cccgtcgagc atgtaggcaa gctgcgcggc cgaaatcgcg
     2401 acgctgccgc cgctcgtcga cggccacacg aaacggccgc gctccagtcg cttggcatag
     2461 agcgacaggc caaggccgtc atgccagagg accttgatca ggtcgcttct cgctccgcga
     2521 aacacgaaaa gatctccggc gaagggatcg cggccaagcg tctcctgcac ctggagcgca
     2581 aggccgttca tgccacgccg catgtcggta cgcccgaccg agagccacac gcgcataccc
     2641 gaggggaccg ggatcatcgg cgcttcagaa cgtcgagaac gcgcctgagc gcacgaggat
     2701 cgaagctcga accgacccga acccggtagt tgcccgcaac gtcgatctcg atcgtctccg
     2761 aagcagccgg cgtgggcacg gctgctccct cgtcctccgg ctcccgcgcg atcgtcaccg
     2821 ccacgagccc gggcgtcgag ggcgaggacg aaggtgtcac gggctcgcca cgataacgtc
     2881 ggcgccagcc gaagacctgg ttcgcgttga catcgtactg ccgggcaacc accgaaaccg
     2941 aggcgccagg aaccaacgtc gccgccacga tctcgcgctt cagcgcctcc ggccagcgct
     3001 tgtaccggcg c
//
LOCUS       DASYJA010000282         1627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2038854, whole genome shotgun sequence.
ACCESSION   DASYJA010000282 DASYJA010000000
VERSION     DASYJA010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2038854"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1543)
                     /locus_tag="VGO02_06070"
     CDS             complement(<1..1543)
                     /locus_tag="VGO02_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEV7477287.1"
                     /translation="MSLEGKTLKVCSCNHTLALDAKALAEALKTGAPLTVHHELCRKD
                     AGAFQAALGAGDDVIVACTQESTLFRELAAESTSKISFVNIRELSGWSAEKSTPKMAA
                     LLAMAALPEPEPAPAVEFKSSGQVLIVGPAAAALDWAERLAEQLEVSVLVTDASGELP
                     LERKFPVWSGSVAQLSGWLGAFEVEWTQENPIDLELCTRCNACIHACPEDAIDFSYQV
                     DLAKCKSHRACVKACGSIGAIDFARSNTSRKDSFDLVLDLSRKPLINLPQLPQGYLAP
                     GADPLEQALAAQQLTALVGEFEKPRFFQYRAKICAHSRSGKEGCNLCLDVCSSGAIQA
                     DGDHVRVEPHLCAGCGGCATVCPSGAMTYAYPKAADTGTRLKTLLSTYRGAGGKDACI
                     LIHDAEDGRLELLSFGRKEKGLPARVIPLEVFHVASLGIDTLLSAISYGASQVVVLST
                     EKVAAGYVAALKKQMQYAQTILTELGYAGKHFSVIEGPSAAWELQPAATVSKPAAFNL
                     SPEKRT"
BASE COUNT          255 a          525 c          535 g          312 t
ORIGIN      
        1 aggtgcgctt ctcgggcgac aggttgaacg cagccggttt gcttacggtc gctgcgggct
       61 gcagctccca cgcggccgac gggccctcaa tcaccgagaa atgctttcct gcatagccga
      121 gctccgtcag gatggtctgc gcgtattgca tctgcttttt cagcgcagcg acgtagcctg
      181 ccgcgacctt ttccgtcgac agcacgacga cctggctcgc gccgtaggaa atcgcgctca
      241 gcagcgtgtc gatgccgagg gaagcgacat ggaatacctc gagcgggatg acgcgcgcgg
      301 gcaagccttt ctcttttctg ccgaaagaaa gcaattcaag gcgaccgtcc tccgcatcgt
      361 ggatcaggat gcaggcgtcc ttgccgcccg cgccgcggta ggtcgagaga agcgtcttca
      421 gccgcgtgcc ggtatcggcc gctttcggat acgcgtaggt catggcgccc gacgggcaga
      481 cggtggcgca gccgccgcag ccggcgcaaa ggtgcggctc caccctgacg tggtcgccgt
      541 cggcctggat cgcgccgctc gagcagacgt cgaggcacag gttgcagccc tccttgcccg
      601 aacggctgtg ggcgcagatc ttcgcgcggt actggaagaa gcgcggcttc tcgaattcgc
      661 ccaccagcgc agtcagttgc tgtgccgcga gcgcctgctc gagcggatcg gcgcccggcg
      721 cgaggtagcc ctgcggcagc tgcggcaggt tgatcagcgg cttgcgcgac aagtccagca
      781 ccagatcgaa cgagtctttt ctcgaagtat tgcttcttgc gaaatcgatc gcgccgatgg
      841 agccgcaggc cttgacgcag gcgcggtgcg acttgcactt cgcgaggtcc acctgatagc
      901 tgaaatcgat cgcgtcttcc gggcaggcat ggatgcaggc attgcagcgc gtgcacagct
      961 cgagatcgat cggattttcc tgggtccact cgacctcgaa ggcgccgagc cagccgctga
     1021 gctgcgccac gctgcccgac cagaccggga atttgcgctc gagcggcagc tcgccggagg
     1081 cgtcggtgac gagcacgctc acctcgagct gctcggcgag gcgctctgcc cagtcgagcg
     1141 ctgcagccgc cgggccgacg atcagcacct ggcccgacga tttgaactcg acggcgggcg
     1201 caggctcggg ctcgggcagc gccgccatgg cgagcagcgc cgccatcttg ggggtggatt
     1261 tctcggccga ccagccgctc agctcgcgga tgttgacgaa gctgatcttc gaagtcgatt
     1321 cggccgcgag ctcgcggaac agcgtgcttt cctgggtgca ggcgacgata acgtcgtcac
     1381 ctgcgccgag cgcggcctgg aacgcgccgg cgtccttgcg gcacagctcg tgatgcaccg
     1441 tcagcggcgc gccggtcttg agcgcctcgg cgagcgcctt ggcgtccagc gccagcgtgt
     1501 gattgcagct gcagacctta agtgtcttgc cttctaggct catcgtcttt ggctttgctg
     1561 ttttcttcgg ccagcttctt ttcttcttcg tcctttttct tcttctcgtc tgccgagaag
     1621 aggagcg
//
LOCUS       DASYJA010000283         3685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_223182, whole genome shotgun sequence.
ACCESSION   DASYJA010000283 DASYJA010000000
VERSION     DASYJA010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223182"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VGO02_06075"
     CDS             complement(<1..311)
                     /locus_tag="VGO02_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEV7477288.1"
                     /translation="MQHFGSYDFIIVGAGSAGCLLANRLSRDPATRVLLLEAGGRDDY
                     FWIDIPVGYLYTIANPRTDWCYRTEPDEHLAGRSIHYARGRVLGGCSSINAMIYMRGQ
                     K"
     gene            345..1094
                     /locus_tag="VGO02_06080"
     CDS             345..1094
                     /locus_tag="VGO02_06080"
                     /inference="COORDINATES: protein motif:HMM:NF016658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HEV7477289.1"
                     /translation="MEPDRQLAEAIDAARDAHFDQHGQACRYPAFAVSRERGRLAAYL
                     ANLETLDFKRLRIPAQTSFWINVFNAGVLRDSPELEFAAGPRDVQAFFEQPRLKIGGH
                     AFSLDEVQHGLLRGNVARHGKLRAPMSRDDPRLAFMPIAFDERMHFALYVAARSSPAL
                     RAFDGGNLDKQLEKATAEHLQRSVRVEQGGAVVVLPRLLDWYAKDFGGERGALEFALG
                     RLDEAAVDSVDRRQGRVKVQYAEYDWTLNRR"
     gene            1214..1783
                     /locus_tag="VGO02_06085"
     CDS             1214..1783
                     /locus_tag="VGO02_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEV7477290.1"
                     /translation="MDSNMVISSRRSFLGNAAKLSAAAVALLAGAESLAQGSGSMLDV
                     NILNVALGLEWEGINAYTLGAQSGLLQKPVLDVAVRFQADHKEHAETLAGAIRKLGGR
                     PVEPKALGDYAKALKADTLKTQADVLELAARLEMGATNAYLGVIPAFKDAKLAQVAAR
                     LAADEVMHWTVLSNALGKPLPAGGMPFGA"
     gene            1784..2152
                     /locus_tag="VGO02_06090"
     CDS             1784..2152
                     /locus_tag="VGO02_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEV7477291.1"
                     /translation="MKRLPLYACAALAAQAVFSQAGAADAERGRQLYESRCIGCHSID
                     ANRVGPAHKGVFGRKAGAIKDYDYSDALKKSRVVWGEKTLGLWLTNPERLIPGQKMGF
                     SVPEAKDRADLIEFLKLQSR"
     gene            complement(2258..2620)
                     /locus_tag="VGO02_06095"
     CDS             complement(2258..2620)
                     /locus_tag="VGO02_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477292.1"
                     /translation="MRTSSLILGGAFALLVSLPAPAQDKDKQPSASPAAGASGSAGAG
                     SSAARRSVPLQANQTRKQLFDKLDSNGNGSISRAEAQQSPALVIIFVETDSNSDGELS
                     AAEFDKVPLVNSDGTPAP"
     gene            complement(2656..2871)
                     /locus_tag="VGO02_06100"
     CDS             complement(2656..2871)
                     /locus_tag="VGO02_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477293.1"
                     /translation="MRSGAFAAALAPSPRSDASIIGPGSVSLPTGVEHPVQLYHYFMR
                     YQPGQPAFGNIPTRCGKDREEVSASRA"
     gene            complement(2971..3435)
                     /locus_tag="VGO02_06105"
     CDS             complement(2971..3435)
                     /locus_tag="VGO02_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477294.1"
                     /translation="MPSAMPARNNGGVLIADVPEMEEVLRSCFSSQVVTYVYYFDAAV
                     RELRVGAFGMVVIGLQFDESRMFQLVQFVRCLPRYDNTPVVCIRGMPSILSAAARKGI
                     RQAVLASGGDAFVDFGLAPQDVDELRQRLADVVRGQATPAVAFDELHSSLRH"
BASE COUNT          614 a         1220 c         1276 g          575 t
ORIGIN      
        1 tctttctgcc cgcgcatgta gatcatggcg ttgatcgacg agcagccgcc caggacgcgg
       61 ccgcgcgcgt agtggatcga gcggccggca aggtgctcgt cgggctcggt gcggtagcac
      121 cagtcggtgc gcgggttggc gatggtgtag agatagccga ccgggatgtc gatccagaaa
      181 tagtcgtcgc gcccgcccgc ctcgagcagc agcacccgcg ttgccggatc ccgggacaag
      241 cggttggcca gcaggcagcc cgcgctgccc gcgccgacga taatgaaatc gtagctgccg
      301 aagtgctgca aaccctgccc ccttgaaggt attgtaacga tgcaatggaa ccggaccgac
      361 agctcgccga ggcgatcgac gccgcgcgcg acgcgcattt cgaccagcac ggacaggcct
      421 gccgctaccc cgcgttcgcg gtgtcgcgcg agcgcggccg ccttgccgcc tatctcgcca
      481 acctcgagac gctcgacttc aagcgcctgc gcatcccggc gcagacctcg ttctggatca
      541 acgtgttcaa cgccggcgtg ctgcgcgact cgccggagct cgagttcgcc gccgggccgc
      601 gcgacgtgca ggcgttcttc gagcagccgc ggctgaagat cggcggccac gccttctcgc
      661 tcgacgaggt gcagcacggg ctgctgcgcg gcaacgtggc gcggcacggc aagctgcgcg
      721 cgccgatgag ccgcgacgac ccgcgcctcg ccttcatgcc gatcgccttc gacgagcgca
      781 tgcatttcgc cctgtacgtc gcggcgcgct cctcgccggc gctgcgcgcc ttcgacggcg
      841 gcaacctcga caagcagctc gagaaggcca ccgccgagca cctgcagcgc agcgtgcgcg
      901 tcgagcaagg cggcgccgtg gtggtgctgc cgagactgct cgactggtac gccaaggact
      961 tcggcggcga gcgcggcgcg ctcgagttcg cgctcggccg gctggacgag gccgcggtcg
     1021 actcggtgga ccgccgccag ggccgggtca aggtgcagta cgccgagtac gactggacgc
     1081 ttaaccggcg ctagcagtac gcgccagcgc atccgcggat gcgctgcccg acgaaaattt
     1141 ctgtgaatcc gctccgcggt gcgccgccgt atcaagcgcg tcggacgtcg acaaataatg
     1201 gagcggagaa gatatggata gcaacatggt catttcatcc cggcgcagct tccttggcaa
     1261 tgcagcgaag ctctcggcgg cggcggtggc gctgctcgcc ggagccgagt cgctggcgca
     1321 gggctcgggc agcatgctgg acgtcaacat cctcaacgtc gcgctcggcc tggagtggga
     1381 aggcatcaac gcgtacacgc tcggcgcgca gagcggcctg ctgcagaagc cggtgctcga
     1441 cgtggcggtg cgcttccagg ccgatcacaa ggagcacgcc gagaccctcg ccggcgcgat
     1501 ccgcaagctc ggcggcaggc cggtcgagcc gaaggcgctc ggcgactacg ccaaggccct
     1561 gaaggccgac acgctgaaga cgcaggcgga cgtgctcgag ctggcggcgc gcctggagat
     1621 gggcgcgacc aacgcctacc tcggcgtgat cccggcgttc aaggacgcga agctcgcgca
     1681 ggtcgccgcg cgcctggccg ccgacgaggt gatgcactgg acggtcctca gcaacgctct
     1741 cggcaagccc ctgcctgcag gcgggatgcc gttcggcgcg taggtgaaac gccttcccct
     1801 gtacgcctgc gcggcgctcg ccgcgcaggc ggttttttcc caggccgggg cggcggacgc
     1861 cgagcgcggc aggcagctct acgagagccg ctgcatcggc tgccattcga tcgacgccaa
     1921 ccgagtcgga cccgcccaca agggcgtctt cggccgcaag gcaggcgcga tcaaggacta
     1981 cgattactcg gatgccctga agaaaagccg ggtggtgtgg ggcgagaaga cgctcgggct
     2041 ctggctgacg aatccggagc gcctcatccc cggccagaag atgggcttct cggtacccga
     2101 ggcgaaggac cgcgcggacc tgatcgaatt cctgaagctg cagtcgcgct aggccgctgc
     2161 gtccgggtcg cgccgcccaa tatccagatg cggcagatct tcgggttggc cgcggcccag
     2221 tcgacgacta tgcgcagcgc gtctgcttca ctgcccacta cggggcgggg gtgccgtccg
     2281 agttgaccag gggaaccttg tcgaactcgg cggcgctcag ctcgccatcc gaattgctgt
     2341 ccgtctcgac gaagatgatc accagcgccg ggctttgctg cgcttcggcg cgcgaaatcg
     2401 agccgttgcc gttcgagtcg agcttgtcga acagctgctt gcgcgtctgg ttggcctgca
     2461 acggcactga gcggcgcgcc gccgagctgc ccgcgcccgc cgaaccgctc gcgccggcgg
     2521 caggggatgc gctgggctgc ttgtccttgt cctgcgccgg cgcaggaagc gacacgagca
     2581 gcgcgaaggc gccgcccagg atcaggctgg aagttctcat ggtgggttct ctcgatgggg
     2641 gatgatgcga gcagcttagg cacggctcgc gctcacttct tccctgtcct tgccgcagcg
     2701 tgtcgggatg ttgccaaagg cgggctggcc gggttgatag cgcatgaagt agtgatagag
     2761 ctgcaccgga tgctcgacac cggtcggcag gctcacgctg cccgggccga tgatggaggc
     2821 gtcgctgcgc gggctcgggg cgagggccgc ggcgaaagcg ccggatcgca cgatcgaccg
     2881 cgtgctgcca ggaaaggacc aggtcatcga gctgcgtccg cacagtttgg gcaggcgggc
     2941 ctgcagcggt attccgcatc cttgcgcaaa tcaatgcctg agcgaggagt gcaattcatc
     3001 gaaggcgacc gcaggcgttg cctgccctct gaccacatcg gcgagccgtt gccgcaactc
     3061 atccacgtcc tggggtgcca ggccgaagtc gacgaaggca tcacccccgc tcgcgaggac
     3121 ggcctgccgg atgcccttgc gtgcggcggc agaaaggatc gagggcattc cccggatgca
     3181 cacgactggc gtgttgtcgt agcggggcag gcagcggacg aactgaacca gctggaacat
     3241 gcgcgactcg tcgaactgca agccgatgac gaccatgccg aatgcgccga cccgcagctc
     3301 gcgcactgca gcgtcaaagt agtacacgta cgtcacgacc tggctgctga aacacgagcg
     3361 caatacctct tccatctccg gcacgtcggc aatcagaacg ccgccgttgt tccgcgcggg
     3421 catggcgctt ggcatggcac gggtttgccc tgcaaccgcc gtgccgagtg gggggtgctt
     3481 gtagtgctaa ttgctacgct ggtgcgctgc ggcgcggaat tagtagaatc ccgacccgat
     3541 gtctccagca ctctccgcca gcccggcatc gtcgcgagcg tcgtcgcggg ccccgaaccg
     3601 gctccgcgtg gtggtgatcg atgacgaacc ggatacggtg ctgatgctgc tcacgcttct
     3661 gcgcgacgag gggtacgagg cagag
//
LOCUS       DASYJA010000284         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1312362, whole genome shotgun sequence.
ACCESSION   DASYJA010000284 DASYJA010000000
VERSION     DASYJA010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312362"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..662)
                     /locus_tag="VGO02_06110"
     CDS             complement(99..662)
                     /locus_tag="VGO02_06110"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEV7477295.1"
                     /translation="MVKVCDCFPELKDITDARERRYRAHPDKPAPEGPRSFVEVKVTS
                     IGQLNELVDCKKSGYKFIISEPVHVGGQNCAPTPLEFLLSGAVGCYAAVFAFYAAKLG
                     VTYDSFEAVARTDFDVRGHMMADAPTSAFQKVTISISVVSDASEDRLKEVERLSFEGC
                     PGINTLREPVPVETRLTVTRTAAEKAA"
     gene            complement(786..1925)
                     /locus_tag="VGO02_06115"
     CDS             complement(786..1925)
                     /locus_tag="VGO02_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEV7477296.1"
                     /translation="MKRSTQRILTTHTGSLPRPTDVVELLLHEQKHPGAEAAKLKDAV
                     RRAVAEVVAKQVACGLDIINDGEQGRTDYTTHVKDRLSGFDGPSSPPLGTGEPDFPEL
                     SAMLAYFASPFQHRPACSGPVGWKDFGALENDIAQAKAAMADTPAQDVFMTSPSPGQI
                     ARYLKNQYYPSEEAYLYALADVMAREYRAIVDAGFILQLDCPDLALSRHMVFPHLSLD
                     EFRKVITTHVEALNHAVRDIPTERMRMHICWASTQGPHHKDVPLKDIVDIVLKGRPQA
                     VSFPGANPRHGHEWKVWKDVKLPEGKIIIPGVIDSTSNFVEHPELVADRIVQYARVVG
                     AGNVIAGVDCGFGTFAGRLQVDSKIVWMKLASLAEGAQLASRELW"
BASE COUNT          311 a          663 c          650 g          376 t
ORIGIN      
        1 gcggtggcga acagcccgag cccggtgcgc gcgcgcccga cgcggtaagg cttgcgcgcg
       61 gcgctgcgcg acgtcttgcg cttcatcacg gctgccgctt acgccgcctt ctccgctgcc
      121 gtgcgcgtca ccgtcagcct ggtctccacc ggcaccggct cgcgcagggt attgattccg
      181 gggcagcctt cgaacgacag gcgttcgacc tccttgagcc tgtcctcgga agcatcgctg
      241 accacgctga tgctgatcgt caccttctgg aaggccgagg tcggcgcatc cgccatcatg
      301 tggccgcgca cgtcgaagtc cgtgcgcgcc accgcctcga agctgtcgta ggtcaccccg
      361 agcttggcgg cgtagaaggc gaacactgcc gcgtagcagc cgaccgcgcc ggacagcagg
      421 aactcgagcg gcgtcggggc gcaattctgc ccgcccacat gcaccggttc gctgatgatg
      481 aatttgtagc cgctcttctt gcagtcgacg agctcgttga gctggccgat cgaggtcact
      541 ttcacttcca cgaagctccg cggtccctcg ggtgccggct tgtcgggatg cgcacggtag
      601 cggcgctcgc gcgcgtcggt gatatccttc aactccggga agcaatcgca gaccttgacc
      661 attcaatctc tcctgagcgc gcttgcggct cgccgttcgc cattcctgcg catcaacttg
      721 gcgagtgaat gcatgcgccg tcaagcctcg ggctttatag gcttccggcg ggccctaccg
      781 cagcttcacc acagctcgcg cgacgcgagc tgtgcgcctt cggcgagcga cgcgagtttc
      841 atccacacga tcttggaatc gacctgcagc cggccggcga aggtgccgaa gccgcaatcg
      901 acgccggcga tgacgttccc ggcgccgacc acgcgcgcat attgcacgat ccgatccgcc
      961 accagctccg ggtgctccac gaagttcgag gtggaatcga tcacgccggg gatgatgatc
     1021 ttgccctcgg gcagcttgac gtccttccag actttccact cgtgcccgtg gcgcgggttg
     1081 gcgcccggaa acgacaccgc ttggggcctg cccttgagca cgatgtcgac gatgtctttc
     1141 agcggcacgt ccttgtgatg gggaccctgg gtcgaggccc agcagatgtg catgcgcatg
     1201 cgctcggttg ggatgtccct gaccgcatga ttgagcgcct cgacgtgggt ggtgatcacc
     1261 ttgcggaact cgtcgaggct caaatgcggg aacaccatgt ggcggctcaa ggccaggtcc
     1321 gggcaatcga gctgcaggat gaagccggcg tccacgatgg cgcggtattc gcgcgccatg
     1381 acgtcggcga gcgcatagag gtaggcttct tcgctcgggt agtattggtt cttgaggtaa
     1441 cgcgcgatct ggcccggcga cggtgatgtc ataaacacgt cctgcgccgg cgtgtcggcc
     1501 atcgccgctt tggcctgggc gatgtcgttt tccagcgcgc cgaaatcctt ccacccgacc
     1561 ggcccggagc aggccggccg atgctggaac ggcgaggcga agtaggccag catcgccgac
     1621 agctcgggaa agtcgggctc gccggtgccg agcggcggcg agctcgggcc atcgaagccg
     1681 ctgaggcgat ccttgacgtg ggtggtgtag tcggtgcggc cctgctcgcc gtcattgatg
     1741 atatcgagcc cgcaggcaac ctgcttcgct acgacctcgg cgacggcgcg gcgcaccgcg
     1801 tccttgagct tggccgcctc ggcgcccgga tgcttctgct catgcagcag caactccacc
     1861 acatcggtcg gccgcggcag gctgccggta tgcgtcgtga gtattctctg cgtgctgcgc
     1921 ttcatgcgtg atcccgccga cagttcccag gcgcgttgcc agtctccaac ggagttgcgg
     1981 ctccaatggc aggaacctca
//
LOCUS       DASYJA010000285         3480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_678053, whole genome shotgun sequence.
ACCESSION   DASYJA010000285 DASYJA010000000
VERSION     DASYJA010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678053"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VGO02_06120"
     CDS             <1..494
                     /locus_tag="VGO02_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477297.1"
                     /translation="RTLPYVDGSRMILAGQSAGAMVSIFTAAMRAPQGLVAVLGFAAG
                     RGGNPEYRPGVPCAVEPLAKLLDTLGKQIKVPVLFHYAQNDRYFNPATTKLWYDRFTA
                     GGAKADYVMQAAFGADGHYVFSAEGGAKIWLPAVEHFLKEYGVPFQTMPPSARPVFAG
                     TAQ"
     gene            complement(499..1986)
                     /gene="gshA"
                     /locus_tag="VGO02_06125"
     CDS             complement(499..1986)
                     /gene="gshA"
                     /locus_tag="VGO02_06125"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEV7477298.1"
                     /translation="MRPLPREVALGLHRGIEKESLRATPAGGLALTPHAPALGSALTH
                     PHITTDFSESQLELITGVHASTEACLEELERIHQFVYRNIGGEILWSASMPCGLPADD
                     AIPIGRYGSSNSGRLKTVYRNGLAWRYGRRMQTISGIHYNFSLPGMTNESHFALIRNF
                     RRVSWLPIFLFGASPAVCESFVSGRSHELETLSESSLFLPHATSLRMGRLGYQSDAQA
                     KLAVSYNSLASYGASLYEALTVPYPPYEKIGIQGPDGEYRQLATSLLQIENEFYTTIR
                     AKRPTRRGERPLHALRERGVEYVEVRLMDLDPFAPLGITAPTMRVLDLLLLHCHLSDS
                     PDDSPQELHAIARNKLHTAQRGREPGLMLERGKERIALTDWGAQILGECEPIAAALDA
                     ALGGAAYRDALAAAQGVVREPESAPSARVLHAMARNHANSYVSFVLAMSLLHQKNDPM
                     PPDAERYFVRLAEKSLEEQRKIEASDSVDFETYRARYLSPELLKL"
     gene            complement(1989..2345)
                     /locus_tag="VGO02_06130"
     CDS             complement(1989..2345)
                     /locus_tag="VGO02_06130"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEV7477299.1"
                     /translation="MPSTTTPSGLKIEEVKVGAGEVAVSGKSVTVHYTGWLTDGVIQG
                     KKFDSSKDRGDPFVFPLGRGQVIKGWDEGVQGMKVGGTRKLTIPPALGYGARGAGGAI
                     PPNATLLFEVELLKVG"
     gene            complement(2374..2799)
                     /locus_tag="VGO02_06135"
     CDS             complement(2374..2799)
                     /locus_tag="VGO02_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV7477300.1"
                     /translation="MKPKSPRSAGAVVFRRTERGIRILLLRAFRNWDFPKGLVETGEE
                     QLDTAKREVAEETGLSDLDWPFGEEFKETLPYGDRKKVARYYLCETGEHDVKLLISAE
                     LGRPEHQEFRWASFDEAEELLPPRLAAVLEWARATITGN"
     gene            2815..3297
                     /locus_tag="VGO02_06140"
     CDS             2815..3297
                     /locus_tag="VGO02_06140"
                     /inference="COORDINATES: protein motif:HMM:NF019416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEV7477301.1"
                     /translation="MGIIQNDSGAVFSACRRWRYLLWRCWDRSGPVANFLMLNPSTAD
                     EAKLDPTCSRARAYAEAWGYGALIVTNIFGWRSTDPRALRRVRDPVGAANDAAILRAA
                     GEADLVVCAWGNHGIERGREVEELLRQTETKLHILKLTGAGHPGHPLYLRKSLKAASW
                     "
BASE COUNT          542 a         1143 c         1204 g          591 t
ORIGIN      
        1 cgcgcacgct gccctacgtc gacggctcgc gcatgatcct cgccggccag tcggccgggg
       61 cgatggtctc gatctttact gcggccatgc gtgcgccgca gggcctggtt gcagtgctcg
      121 gtttcgccgc cgggcgcggc ggcaacccgg agtaccgccc cggcgtgccc tgcgcggtcg
      181 agccgctggc gaagctcctc gataccctgg gcaagcagat caaggtgccg gtgctgttcc
      241 attacgcgca gaacgaccgc tacttcaacc cggccaccac caagctctgg tacgaccgct
      301 tcaccgcggg cggcgccaag gccgactatg tgatgcaggc ggcgttcggc gccgatggcc
      361 actatgtctt cagcgccgag ggcggcgcga agatctggct gccggcggtg gagcacttcc
      421 tgaaggaata cggcgtgccc ttccagacca tgccgccctc ggcgcggccg gtgttcgccg
      481 gcaccgcgca gtagccgcct agagcttcag cagctcgggc gagaggtagc gcgcccggta
      541 ggtctcgaag tcgacgctgt cggacgcttc aattttcctt tgctcttcca gggatttctc
      601 tgccaatcga acgaaatacc gttcggcgtc cggaggcata ggatcgtttt tttggtgaag
      661 caaggacatg gcgaggacga agctcacgta ggagttggcg tggttgcgcg ccatggcgtg
      721 cagcacgcgc gccgagggcg ccgactcggg ctcgcgcacg acgccctgcg ccgcggccag
      781 cgcgtcgcgg taggcggcgc cgcccagcgc ggcgtcgagc gccgcggcga tcggctcgca
      841 ctcgccgagg atctgcgcgc cccagtcggt cagcgcgatg cgctccttgc cgcgctccag
      901 catcagcccc ggctcgcggc cgcgctgcgc cgtgtgcagc ttgttgcggg cgatggcgtg
      961 cagctcctgc ggcgagtcgt ccgggctgtc gctcaggtgg cagtgcagga gcagcagatc
     1021 gagcacgcgc atggtcggcg cggtgatccc cagcggcgcg aacgggtcga ggtccatcag
     1081 gcgcacctcg acgtactcga cgccgcgctc gcgcagcgcg tgcagcggcc gctcgccgcg
     1141 ccgggtcggc cgcttggcgc ggatggtggt gtagaactcg ttctcgatct gcagcaggct
     1201 ggtggcgagc tggcggtact cgccgtcggg tccttggata ccgatcttct cgtagggcgg
     1261 ataaggcacc gtgagcgcct cgtacagcga ggcgccgtag ctcgccaggc tgttgtagct
     1321 caccgccagc ttcgcctgcg cgtcgctctg gtagcccagg cgtcccatgc gcagcgaggt
     1381 ggcgtgcggg agaaacaggc tgctctcgct aagagtctct aattcatgag agcgacccga
     1441 aacaaagctc tcgcacaccg ccggcgaggc gccgaacagg aaaatcggca gccaagagac
     1501 gcgccggaaa ttgcggatca gcgcgaagtg gctctcgttg gtcattcccg gcagcgagaa
     1561 gttgtagtgg atgcccgaga tggtctgcat gcgccggcca tagcgccagg cgaggccgtt
     1621 gcggtagacg gtcttcaggc ggccgctgtt cgagctgcca tagcgcccga tcgggatggc
     1681 gtcatcggcc ggcaggccgc agggcatgct cgcgctccac aggatctcgc cgccgatgtt
     1741 gcggtagacg aactggtgga tgcgctcgag ctcctccagg caggcctcgg tgctggcgtg
     1801 cacgccggtg atcagctcga gctgcgactc gctgaagtcg gtggtgatgt gcgggtgggt
     1861 gagcgccgag cccagggccg gcgcgtgcgg cgtgagcgcg aggccgcctg cgggcgtggc
     1921 gcgcaggctc tccttctcga tgccgcggtg cagcccgagg gccacctcgc ggggcagcgg
     1981 tctcaaaatc aaccgacttt tagaagctcg acttcgaaca gcaaggtcgc attcggcgga
     2041 atcgcgccgc cggcgccgcg cgcaccgtag ccgagcgccg gggggatcgt gagcttgcgc
     2101 gtgccgccca ccttcatgcc ctgcacgccc tcgtcccagc ccttgatcac ctggccgcgg
     2161 ccgagcggga agacgaacgg gtcgccgcga tccttgctcg agtcgaactt cttgccctgg
     2221 atgacgccgt cggtcagcca gccggtgtag tgcacggtga cgcttttccc ggaaacggcg
     2281 acctcgccgg cgccgacttt tacttcctcg atcttcagcc cgctgggcgt ggtggtgctt
     2341 ggcatggcct tccttcggag aaaaagcgta attttagttg cctgtgatgg tcgcgcgcgc
     2401 ccattcgagc accgccgcca gccgcggcgg cagcagctcc tcggcctcgt cgaagctcgc
     2461 ccagcgaaac tcctggtgct cggggcggcc gagctcggcg gagatgagca gctttacgtc
     2521 gtgctcgcct gtttcgcaca ggtaatagcg cgccaccttc ttgcgatcgc cgtagggcag
     2581 ggtttccttg aattcctcgc cgaagggcca gtccaggtcg ctcaggccgg tctcctccgc
     2641 cacctcgcgc ttggcggtgt cgagctgctc ctcgccggtc tccaccaggc ccttggggaa
     2701 gtcccagttc ctgaacgccc gcagcagcag gatgcggatg ccgcgctcgg tgcggcggaa
     2761 caccaccgcc ccggccgaac gaggactctt gggcttcatg cggataatgg tagcgtggga
     2821 atcatccaaa acgactcggg tgccgtgttc tcggcatgcc gccgctggcg ctacctgctg
     2881 tggcgatgct gggatagatc cggcccggtc gccaacttcc tgatgctcaa cccgagcacc
     2941 gccgacgagg cgaagctcga cccgacctgc tcgcgggcgc gcgcctacgc cgaggcctgg
     3001 ggctacggcg ccctgatcgt gacgaatatc ttcggctggc gctcgaccga tccgcgcgcg
     3061 ctcaggcgcg tgcgcgaccc ggtcggggcg gccaacgatg cggcgatcct gcgggctgcg
     3121 ggagaagctg acctggtggt ctgcgcgtgg gggaaccacg gcattgaaag aggcagggaa
     3181 gtcgaagaac tgctcagaca gacagaaacg aaactacata ttctcaaact gaccggagcc
     3241 ggccatccgg gacatccgct ctaccttcgg aaatccctca aggcggcatc ctggtgagag
     3301 atgcccgccg cccgcgcgag ccctagccag aggaggagga ggaggcgtcg gatttgcgcg
     3361 gacggcgaag caagcttgaa tctttgggtt gcaacccctg ggtctagtgg gcgccggtgg
     3421 gcgagcgaaa cagtttgctc agcccggaac aagcgccggc ccaggcgcgc gatatggttt
//
LOCUS       DASYJA010000286         5676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2039030, whole genome shotgun sequence.
ACCESSION   DASYJA010000286 DASYJA010000000
VERSION     DASYJA010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5676
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2039030"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VGO02_06145"
     CDS             complement(<1..405)
                     /locus_tag="VGO02_06145"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEV7477302.1"
                     /translation="MRFLRLLLSLAALLACAAAWSQAYPARPVRLLVPFAPGGTTDVL
                     ARLVGQKLSEALGQQFVIDNRPGAGGNIGTELAAKSPADGYTLVMSFDGTMAINPSTY
                     RQLPFDAQRDLAPVASVAQVPLLIVVHPGVAAN"
     gene            complement(409..1380)
                     /locus_tag="VGO02_06150"
     CDS             complement(409..1380)
                     /locus_tag="VGO02_06150"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7477303.1"
                     /translation="MGKRQANTLRIVLLAVLFAAGGAFAQYPSRPVRIVVTIPPGGAP
                     DIAARIIGQRLGEGLGQPFIIDNRPGANGNTAAAEVARAPADGYTLILAADSLIAINP
                     HLYSRMPLDTLKDLGPVASLVSNQFVLSVNPSLPVKTFPEFIEYARKANPPLNYASGG
                     NGSQHQLTMEMLKQRAGIDLVHVPYRGGAPATTATVAGEVAAMFAGTSTAPQIKAGRL
                     RALAVAGARRSRAFPELPTIAEFYPGFENSIWLGLFGPAGLPEEVLLKLRNEITRTLQ
                     APEVIEKLHGAGSLEPFITTPEEFAALVRRDYERYGKVIKEVGVKAD"
     gene            complement(1388..2362)
                     /locus_tag="VGO02_06155"
     CDS             complement(1388..2362)
                     /locus_tag="VGO02_06155"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7477304.1"
                     /translation="MPVRLLLWLFVAAVSSAALAQPAVVQSYPSKPIRLVSPFPPGGS
                     VDVVGRLLAAKLSESLGQQMVVDNRSGASGVVGSEAVLNSPPDGYTLLINTIPFVTNQ
                     FLLPRAPYDPLRDFVSISIVASAPSFVTVHPSVPARSVQELIALAKAKPGQLFYSAAG
                     VGTNPHIAGELFNLLANVDIVAVQFKGGGPADTAVIAGEVQATFGNVSQQIGHVKSGR
                     LRALAVTSAQRNPAMPELPTVAEAGVPGYEFDTWFVVVAPRGTPRPIVDTLHSHIRKV
                     LTPPEQVKFWQERGLSVVAGTPEEAAAYLESEQKKWARVIKERGIKAE"
     gene            complement(2367..2443)
                     /locus_tag="VGO02_06160"
     tRNA            complement(2367..2443)
                     /locus_tag="VGO02_06160"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2407..2409),aa:Val,seq:gac)
     gene            2478..2924
                     /gene="sixA"
                     /locus_tag="VGO02_06165"
     CDS             2478..2924
                     /gene="sixA"
                     /locus_tag="VGO02_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HEV7477305.1"
                     /translation="MDLILWRHAEAEEGGPDLARSLTGKGRKQAARVAEWLQQRLPAK
                     FTLLASPALRAQQTAEALGARIKTVEKLAPGARVPDIIAAAGWPEHKGVVVVVGHQPD
                     LGNAVAYLLSGSAADWSLKKGALCWLASRDKEPRVVLRAAISPDLL"
     gene            complement(2907..4439)
                     /gene="ppx"
                     /locus_tag="VGO02_06170"
     CDS             complement(2907..4439)
                     /gene="ppx"
                     /locus_tag="VGO02_06170"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEV7477306.1"
                     /translation="MARHDTLAAVDLGSNSFHLQIGRVVDGQIYPLDAVREVVRLGGG
                     LTADKRIDRATQAAALEALAKFAERLRGFPRQAVRAVGTNALRVAKNSPQFLREARAA
                     LGFPIEVISGREEARLIYLGVAHSLPAAALSKTERRLVFDVGGGSTEFIIGTGLEPQL
                     TESLYMGCVSYSLKYFPGGRIEKSAMKAAETAARQELAGIVRSYRAEGWEEAVASSGT
                     ARSIENILRENELAEEGITRDGLEKLRTILIKNEKADPDRIAGLRSNRAPVLPGGVAI
                     MSAVFDELGLDSVKVSDGALRHGVLYDLLGRVQHRDMREATVAQFMRRYHVDAAQAER
                     VKNLSLVIYDGLLPGAEREDDADRTMLEWAARLAEIGLSIAHAQYHKHSAYVLSNADM
                     PGFSRMEQARLARVVLAHRGKLGKVQDLGFEGADWKLVFALRCASLILRRRADLKLPF
                     LRVAADSGGFAIDLPQSWLEDNPLSADALESEADHWKAVGMKLEVGALSDKKVSVLKQ
                     VR"
     gene            complement(4446..5141)
                     /gene="phoU"
                     /locus_tag="VGO02_06175"
     CDS             complement(4446..5141)
                     /gene="phoU"
                     /locus_tag="VGO02_06175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV7477307.1"
                     /translation="MNSHTSTEFDELMARAHQLVVQMGERVKRQVEDAVESLVSGSPA
                     LINQILRHEAVVNALEREIDELAGQIIARRQPTAGDLRFVLAVVNLTTDLERVGDEAK
                     KIALQARKMSADGKLTQPRTLEVRRMAAIVQDMLSAAITALETLEPRAAVALARRDEE
                     LDLIFQGVLRQLISFMIEDPRTITACLDMLFVAKSLERIGDHAVNVSEHVMYAATGRD
                     VRHASAEEKSVIP"
     gene            5228..>5676
                     /locus_tag="VGO02_06180"
     CDS             5228..>5676
                     /locus_tag="VGO02_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEV7477308.1"
                     /translation="MNQRIPPPTQFLNRELGILAFNRRVLAQAADEAVPLLERLRFIT
                     IVSSNLDEFFEIRVAGLKEQIKLGLPEPGPDGRGPAEVFAAVAAGAHALVAEQYKLLN
                     EVVLPALAREGVVFPKREEWSAAQRQWIRDYFFRELLPVLTPIGLDPA"
BASE COUNT          878 a         1913 c         1988 g          897 t
ORIGIN      
        1 gttcgccgcc acacccggat gcaccacgat gagcagcggc acctgcgcca cgctcgccac
       61 cggcgcgagg tcgcgctgcg cgtcgaacgg cagctggcgg taagtgctcg ggttgatcgc
      121 catggtgccg tcgaagctca tcaccagagt atagccgtcg gcgggcgatt tcgccgccag
      181 ctcggtgccg atgttgccgc cggcaccggg gcggttgtcg atgacgaact gctggccgag
      241 cgcttcggac agcttctgtc cgaccagccg cgccagcacg tcggtcgtgc cgcccggcgc
      301 gaaaggcacc agcagccgca ccggcctggc gggataggct tgcgaccagg ccgcggcgca
      361 agccagcagc gcggcgagcg acagcagcag gcgcaggaaa cgcatcgcct agtcggcctt
      421 gacgccgact tccttgatca ccttgccgta gcgctcgtag tcgcggcgga ccagcgcggc
      481 gaactcttcg ggcgtggtga tgaagggctc gaggctgccc gcgccgtgca gcttctcgat
      541 cacctcgggg gcctgcagcg tcctggttat ttcatttctc aatttcaaaa gaacctcttc
      601 cggcagtccc gcggggccga acaggcccag ccagatcgag ttctcgaagc ccggatagaa
      661 ctcggcgatg gtcggcagct ccggaaaggc gcgcgagcgc ctggcgcccg ccaccgccag
      721 cgcgcgcagg cggccggcct tgatctgcgg cgcggtcgag gtgccggcga acatggcggc
      781 cacttcgccg gcgaccgtgg cggtcgtcgc gggcgcgccg ccgcggtaag gcacgtgcac
      841 caggtcgatg ccggcgcgct gcttgagcat ctccatggtc agctggtgct ggctgccgtt
      901 gccgcccgag gcgtagttga gcggcggatt cgccttccgc gcgtactcga tgaactcggg
      961 gaaagtcttc accggcagcg acgggttgac cgagaggacg aactggttgc tgaccaggct
     1021 cgccaccggg ccgaggtcct tgagcgtatc gagcggcatg cgcgagtaca gatgcggatt
     1081 gatcgcgatc aggctgtcgg cggcgaggat cagcgtgtag ccgtcggccg gcgcgcgcgc
     1141 cacctcggcc gccgcggtgt tgccgttggc gcccggccgg ttatcgatga tgaacggctg
     1201 gccgaggcct tcgcccagcc tctgcccgat gatgcgcgcc gcgatgtcgg gcgcgccgcc
     1261 cggcgggatg gtgaccacga tgcgcaccgg gcgcgacgga tactgggcaa aggcgccgcc
     1321 cgcggcgaag aggacggcga gtagaacgat gcgaagcgtg tttgcctgcc tttttcccat
     1381 atggggatta ctccgccttg atgccgcgtt ccttgatgac gcgcgcccat ttcttctgct
     1441 cgctctcgag gtacgcggcc gcttcctcgg gcgtgccggc gaccacgctc agtccgcgct
     1501 cctgccagaa cttcacctgc tccggcgggg tgagcacctt gcggatgtgg gaatgcagcg
     1561 tatcgacgat cgggcgcggc gtgccgcggg gcgccacgac cacgaaccag gtgtcgaact
     1621 cgtagcccgg cacgcccgcc tcggcgacgg tcggcagctc gggcatcgcg ggattgcgct
     1681 gcgcgctggt gacggcgagc gcacgcaggc ggccggattt cacgtggccg atctgctgcg
     1741 agacgttgcc gaacgtggcc tgcacctcgc ccgcgatgac ggccgtgtcg gcgggtccgc
     1801 cgcctttgaa ctgcaccgcc acgatgtcga cgttcgccag caggttgaac agctcgccgg
     1861 ctatatgcgg attggtgccg acgccggcgg cggagtagaa cagctggccc ggcttcgcct
     1921 tggcgagcgc gatgagctcc tgcaccgagc gcgccggaac cgacggatgc accgtgacga
     1981 acgagggcgc cgacgcgaca atcgagatgg aaacgaaatc gcgcagcggg tcgtagggcg
     2041 cgcgcggcag caggaactgg ttggtgacga aggggatggt gttgatgagg agcgtgtagc
     2101 cgtcgggcgg cgagttgagg accgcctcgc tgccgaccac cccggaggcc ccggaccggt
     2161 tgtcgacgac catctgctgg ccgagggact cggagagctt cgccgcgagc agccggccga
     2221 cgacgtcgac gctgccgccc ggcggaaacg gcgagaccag ccggatcggc ttcgatggat
     2281 aggactggac aacggcaggc tgcgccagcg cggcgctgga aaccgctgca acgaacagcc
     2341 agagcaagag tctgacgggc atggattggt aggcgcgatt ggactcgaac caacgacccc
     2401 caccatgtca aggtggtgct ctaaccagct gagctacgcg cctgctgtaa caaggcgaat
     2461 tttatactac gggatatatg gacctgatcc tctggcgcca cgccgaggcc gaggaaggcg
     2521 gcccggatct cgcgcgctcg ctcaccggca aaggccgcaa gcaggcggcg cgcgttgccg
     2581 aatggctgca gcagcgcctg ccggcgaagt tcacgcttct cgccagcccg gcgctgcgcg
     2641 cgcagcagac cgcggaggcg ctcggcgcgc gcatcaagac ggtggaaaag ctcgcgccgg
     2701 gcgcgcgcgt gcccgacatc atcgccgcgg cgggatggcc cgaacacaag ggcgtagtgg
     2761 tggtggtcgg gcaccagccc gacctcggca acgcggtggc ctatctgctg tcgggctcgg
     2821 ctgccgactg gagcctcaag aagggcgcgt tgtgttggct ggcaagccga gacaaggaac
     2881 cccgagtcgt cctgagagcc gcgatttcac ctgacctgct ttagcaccga aaccttcttg
     2941 tcggagagcg cgccgacctc cagcttcatg ccgacggctt tccagtgatc ggcctccgac
     3001 tcgagcgcgt ccgccgagag cgggttgtcc tcgagccatg actgcggcag gtcgatggcg
     3061 aagccgccgc tgtcggcggc gacgcgcagg aacggcagct tgaggtcggc gcgccggcgc
     3121 aggatgagcg aggcgcagcg cagcgcgaac accagcttcc agtccgcgcc ctcgaagccg
     3181 aggtcctgca ccttgccgag cttgccgcgg tgcgcgagca cgacgcgcgc gaggcgcgcc
     3241 tgctccatgc gcgagaaccc cggcatgtcg gcgttcgaca gcacgtaggc ggagtgcttg
     3301 tggtactgcg cgtgcgcgat cgacaggccg atctcggcga ggcgcgccgc ccactcgagc
     3361 atggtgcggt cggcgtcgtc ctcgcgctcg gctccgggca gaagtccatc gtaaatcacc
     3421 agggacaggt tcttgacgcg ctcggcctgc gccgcgtcga cgtggtagcg ccgcatgaac
     3481 tgcgccaccg tcgcttcgcg catgtcgcgg tgctgcacgc gcccgagcag gtcgtacagc
     3541 acgccgtggc gcagggcacc gtcggacacc ttgacgctgt cgaggccgag ctcgtcgaac
     3601 accgcgctca tgatggcgac gccgccgggc agcaccgggg cgcggttgga ccgcagcccg
     3661 gcgatgcgat cggggtctgc cttttcgttt tttatgagga tcgttctcag tttttccaag
     3721 ccatccctgg taatgccttc ctcggcgagc tcgttctcgc gcaggatgtt ctcgatcgag
     3781 cgcgcggtgc cggaggacgc caccgcctcc tcccagccct cggcgcggta gctgcgcacg
     3841 atgccggcga gctcctggcg cgcggccgtc tcggcggcct tcatggcgga cttctcgatg
     3901 cgcccgccgg ggaaatactt caggctgtag ctgacgcagc ccatgtacag cgactcggtg
     3961 agctgcggct ccaggcccgt gccgatgatg aactcggtcg agccgccgcc gacgtcgaac
     4021 accaggcgcc gctcggtctt cgacagcgcc gcggcgggaa gagagtgcgc gacgccgagg
     4081 tagatgaggc gcgcctcctc gcggccggaa atcacctcga tcgggaagcc cagggcggcg
     4141 cgcgcctcgc gcaggaactg cggcgagttc ttcgccacgc gcagcgcgtt ggtgcccacc
     4201 gcgcgcaccg cctggcgcgg gaagccgcgc aggcgctcgg cgaacttggc gagcgcctcg
     4261 agcgcggccg cctgcgtggc gcggtcgatg cgcttgtcgg cggtcagccc gccgcccagg
     4321 cgcaccacct cgcgcaccgc gtcgagcgga tagatctggc cgtcgacgac gcggccgatc
     4381 tgcaggtgga agctgttgga gccgaggtcg acggcggcga gcgtatcgtg gcgggccatc
     4441 gccgcctacg gtatcaccga cttctcctcc gcggaagcgt gccgcacgtc ccggccggtg
     4501 gccgcgtaca tgacgtgctc ggagacgttc accgcgtgat cgccgatgcg ctcgagcgac
     4561 ttggcgacga acagcatgtc aaggcaggcg gtgatggtgc gcgggtcctc gatcatgaag
     4621 ctgatcagct ggcgcagcac gccctggaag atcaggtcca gctcttcgtc gcgccgcgcg
     4681 agagcaacgg ccgcgcgcgg ctcgagcgtc tcgagggcgg tgatcgccgc gctcagcatg
     4741 tcctggacga tggccgccat gcgccgcact tcgagggtgc gcggctgcgt cagcttgccg
     4801 tcggcggaca tcttgcgcgc ctgcagcgcg atcttcttcg cctcgtcgcc gacgcgctcg
     4861 aggtcggtag tgagattgac caccgccagc acgaagcgca ggtcgcccgc ggtgggctgg
     4921 cggcgcgcga tgatctgccc ggccagctcg tcgatctcgc gctcgagcgc gttcaccacc
     4981 gcctcgtggc gcaggatctg gttgatcagc gccggcgagc ccgagaccag gctttcgacc
     5041 gcgtcttcca cctgccgctt cacacgctcg cccatctgca cgaccagctg gtgggcgcgt
     5101 gccatcagct catcgaattc cgtcgaggtg tgggaattca tgaccggttg acgctaacgg
     5161 ccgactgtta caggaacatt tcactcattt gtcccccgcg tccggcggta aaattattgg
     5221 ctccgccgtg aatcagagaa ttcccccgcc gacccagttc ctcaaccgcg agctcggcat
     5281 cctggcgttc aaccgccgcg tgctggcgca ggcggccgac gaggcggtgc cgctgctcga
     5341 gcgtctgcgc ttcatcacca tcgtgtcgtc gaacctggac gaattcttcg agatccgcgt
     5401 cgccggcctg aaggagcaga tcaagctcgg cctgcccgag ccggggcccg acggccgcgg
     5461 tccggccgag gtgttcgccg cggtcgccgc cggtgcgcac gcgctcgtcg ccgaacagta
     5521 caaattgctc aacgaagtcg tgttgccggc cctcgcgcgc gaaggcgtgg tgttcccgaa
     5581 gcgcgaggaa tggagcgccg cgcagcgcca gtggatccgc gactatttct tccgcgagct
     5641 gctgccggtg ctgacgccga tcgggctcga cccggc
//
LOCUS       DASYJA010000287         6071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1131839, whole genome shotgun sequence.
ACCESSION   DASYJA010000287 DASYJA010000000
VERSION     DASYJA010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131839"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..812
                     /locus_tag="VGO02_06185"
     CDS             285..812
                     /locus_tag="VGO02_06185"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7477309.1"
                     /translation="MSENVALRPAGPADIPAIHAIYEHHVLKGLASFEEEPPSQEELL
                     RRYRDVTGGGLPYIVAELGGALAGYGYCALYRSRSAYRYALEDSVYVRHDMHGRGVGS
                     ALLADLIRRCESLGYRQLIAVIGDSAHAASIGLHASLGFLRVGTLRAVGFKFGRWVDS
                     VIMQRPLGPGDSTTP"
     gene            812..2386
                     /locus_tag="VGO02_06190"
     CDS             812..2386
                     /locus_tag="VGO02_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEV7477310.1"
                     /translation="MKRFQVIVVGAGPVGAALALDLGLRGISCALIESRTGLHRIPKG
                     QNLTQRTLEHFHFWGIADALRAARVMPRGYPIGEITAYGDLMSPYWHAPAGREVVRPF
                     YFQDNERLPQYCMEAVLRERIASLSGVATRFGWTARSVEQGDDIVRVAIAGEGGRSEV
                     LEADYVVGCDGGQSLVRREAGIERSGTDFQQPMALMVFRSRELHEGLKRFPERSTYRV
                     MRDDLKGYWWFLGRIDVGEGWFFHAPVPVGTERDNFDFHGLAQRAAGFAFAWQVEHIG
                     FWDLRVSLAAHYRVGRILIAGDAAHSHPPYGGFGLNNGLEDAVNLGWKLQAMLEGWGG
                     ETLLRSYDEERRPVFKAVGDDFIAARIRHDAEFFERFNPQRDRAQFERAWAGRENELG
                     ARAQSYEPNYEGSPVVAGPAGGVCSAHGRHEFKARAGHHLAPQLLSTGRNVYEELSRS
                     AFTLLAFDTESAGFEAAARSLRLPLKIVRDSYREGRAAYESRLVLVRPDQFVAWAGDH
                     APQDCAALLASAAGRA"
     gene            complement(2387..3364)
                     /locus_tag="VGO02_06195"
     CDS             complement(2387..3364)
                     /locus_tag="VGO02_06195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEV7477311.1"
                     /translation="MKAVVCKQFGPPDSLVVEDMPSPRAGPGEVVLSVKAASVNFPDV
                     LIIQNKYQFKPPLPFSPGSELAGVVKETGAGVEGFKPGDKVIAFTTYGAFAEEVKTEA
                     ARLLPLPQGMDFATGAAFLLTYGTSDHALRDRGALRAGETLLVLGAAGGVGLAAIDIG
                     KALGARVIACASSEDKLAVCRSHGADEAINYSTEDLRERIQAITGKSGVDVVYDPVGG
                     PYSEPAFRSLAWRGRLLVVGFAAGDIPKLPLNLALLKGASLVGVFWGDFTRREPRQFA
                     DSVRQLGEWYRQGKLRPHVSQTFPLEKAAEALKLMAARQVKGKLVLTVG"
     gene            3382..4320
                     /locus_tag="VGO02_06200"
     CDS             3382..4320
                     /locus_tag="VGO02_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEV7477312.1"
                     /translation="MQELILHHYPVSPFAEKIRTILGYKKLAWRSVEIPMVLPKPDVL
                     ALTGGYRRTPLLQIGADVYCDTALIARTLERIAPEPSIFPQSETLAVQAAAFFADNVL
                     FNIAVPIGFQPGGMLKLFLPDASSEFLDRFARDRAAMRQGGTVRRGPLAECKANLAHT
                     LARVEAQLQGPFLFGARPTVADFSLYHVLWSVWKPPIARPLLDPFPKTVALVERIAAF
                     GHGRPLPLGSTDALEISKKARPAPIEKPQAVETGGVALGEMAEVMPVDSALDPVRGEL
                     VHCSVDEIVLRRSDPRAGTIAVHFPRFGYQMRPCQA"
     gene            4308..5198
                     /locus_tag="VGO02_06205"
     CDS             4308..5198
                     /locus_tag="VGO02_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7477313.1"
                     /translation="MPSLSTDDGVKLYYEEAGAGTPIVFVHEFAGDHRSWEPQLRHFS
                     RRYRCIAYNARGYPPSEVPQDVERYSQARWRDDIRCVLDALGIQRAHVVGLSMGAFAS
                     LHFGITYGYRALSITVAGGGYGAHPAQYRKFQEDSRTNAAFIQREGMAKFAATYGHGP
                     TRVQFQNKDPRGFAEYIGQLTEHSALGSANTMLGYQSRRPSLYDLTDEMARIEVPTLI
                     MAGDEEEPCLEVCLLMKRAIPTAGLAVLPRSGHGINLEEPALFNRLLEDFFRQVEAGR
                     WGRRDPRATVPSLYGPGGKP"
     gene            5195..5995
                     /locus_tag="VGO02_06210"
     CDS             5195..5995
                     /locus_tag="VGO02_06210"
                     /inference="COORDINATES: protein motif:HMM:NF004781.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV7477314.1"
                     /translation="MTSSSSEGKLTVEKRGAVGWIVFDSPAKRNAINGAMWRGIAPAM
                     ARFDADPEVRCVAFRGSGREAFSAGADISEFDTIRAQNSAVSEYDGLLDQVLHAIQDS
                     RKASVAMIHGFCMGGGLEIALACDLRYCGASAQFGIPAAKLGLAYNVEGHRRLLETVG
                     HARAREIMFLGGRYIAADALAMGLVNQVLPDDALEKHTAEILQTLSDNAPLAIANSKT
                     IIEEYAKSQGAPDAAAMQRAMQRCIDSEDYKEGRRAFMEKRKPRFTGR"
BASE COUNT          950 a         2149 c         2030 g          942 t
ORIGIN      
        1 ccaatggagc gatcgttcac caggaacgtc atcgagcgca tctgcacccc ccctttgccc
       61 cgttgcgagg caatctgcga aatgattcgc cgtcagccgg cgccgcacaa tcccgtgttt
      121 atgtgagcgc gatcgatgga catcggaccg ctgccgtccc tgatacgctc cccctgcgaa
      181 agtctatttc atctccatgc aaaaaattca tgacgcgctg cggcaatcac gggatctatc
      241 cgttcgtccg ccggaactac caaaaatcat tggaaatcaa atcgatgtct gaaaatgttg
      301 cattgcggcc ggcgggcccc gccgacatcc cggcgatcca cgccatctac gagcatcacg
      361 tcctgaaggg cctcgcctcc ttcgaggaag agccgccgtc ccaggaggag ctgctgcgcc
      421 gctatcgcga cgtcaccggc ggcggcctgc cttacatcgt cgccgagctc ggcggcgccc
      481 tggccggcta cggctactgc gcgctctatc ggtcgcgctc ggcgtaccgc tacgcgctcg
      541 aggactcggt ctacgtccgg cacgacatgc acggccgcgg cgtcggttcg gcgctgctcg
      601 ccgatctgat ccggcgctgc gagagcctcg gctaccggca gctcatcgcg gtcatcggcg
      661 acagcgcgca cgccgcctcg atcggcctgc acgccagcct cggcttcctg cgcgtcggca
      721 cgctgcgcgc ggtgggcttc aagttcggcc gctgggtgga cagcgtgatc atgcagcgtc
      781 cgctcggccc cggcgacagt acgacgccgt agtgaagcgg ttccaggtca tcgtcgtggg
      841 cgctggaccg gtcggcgccg ctctcgcgct cgacctcggg ctgcgcggca tctcgtgcgc
      901 cctgatcgag agccgcaccg ggctgcaccg catcccaaag ggccagaacc tcacccagcg
      961 cacgctcgag catttccact tctggggcat cgccgatgcg ctgcgcgccg cccgcgtcat
     1021 gccgcgcggc taccccatag gcgagatcac cgcgtacggc gacctgatga gcccctactg
     1081 gcacgctccg gcgggccggg aagtcgtccg gcctttctat ttccaggaca acgagcggct
     1141 gccgcagtac tgcatggagg cggtgctgcg ggagcgaatc gcctcgctgt ccggcgtggc
     1201 aacccgcttc ggctggaccg cgcgctcggt ggagcagggc gacgacatcg tgcgcgtcgc
     1261 gatcgccggc gagggcggac gcagcgaagt cctggaggcc gactacgtgg tgggctgcga
     1321 cggcggccag tcgctggtgc gccgcgaggc cggcatcgaa cgcagcggca ccgattttca
     1381 gcagccgatg gcgctgatgg tatttcgctc gcgcgagctg cacgagggcc tcaagcgctt
     1441 tcccgagcgc tcgacctacc gcgtgatgcg cgacgacctc aagggctact ggtggttcct
     1501 cggcaggatc gatgtcggcg aaggctggtt cttccatgcg ccggtgccgg tcggcaccga
     1561 gcgcgacaac ttcgatttcc acggcctcgc ccagcgcgcc gccggctttg ccttcgcctg
     1621 gcaggtggag cacatcggtt tctgggatct gcgcgtgtcg ctcgccgcgc actatcgggt
     1681 cggccgcatc ctcatcgccg gcgacgccgc gcacagccac ccgccctacg gcggcttcgg
     1741 cctcaacaac ggcctcgagg acgcggtcaa cctgggctgg aagctccagg ccatgctcga
     1801 gggctggggc ggcgagacgc tgctgcgctc ctacgacgaa gagcggcgtc cggtcttcaa
     1861 ggcggtggga gacgacttca tcgccgcgcg catccggcac gacgcggagt tcttcgagcg
     1921 cttcaatccg cagcgcgacc gggcgcaatt cgagcgcgcc tgggcggggc gcgagaacga
     1981 gctcggcgcg cgcgcgcaga gctacgagcc gaactacgaa ggctcgccgg tggtcgccgg
     2041 ccccgcgggc ggcgtctgca gcgcgcacgg ccggcacgaa ttcaaggcgc gggcggggca
     2101 ccacctcgcg ccgcagctgc tttccaccgg ccgcaatgtt tacgaggagc tttcgcgcag
     2161 cgcattcact ctgctcgcgt tcgataccga gagcgcgggt ttcgaggcgg ccgcgagatc
     2221 gctgcgcctg ccattgaaaa tcgtgcggga ttcctaccgg gaaggccgcg cggcatacga
     2281 gtcgcggctg gttctcgtca ggccggacca gttcgtcgcc tgggccgggg atcacgcgcc
     2341 gcaggattgc gcggcgctgc tcgcaagcgc ggccgggcgc gcctgatcag ccgacggtca
     2401 ggaccagctt tcccttcacc tgccgcgccg ccatcagctt gagcgcctcg gccgccttct
     2461 ccagcgggaa ggtctgggag acgtgcggcc gcagcttgcc ctgccggtac cactcgccga
     2521 gctggcgcac gctgtcggcg aactgcctgg gctcgcggcg cgtgaaatcg ccccagaaca
     2581 cgccgaccag cgaagcgccc ttgagcagcg cgaggttgag cggcagcttc gggatgtcgc
     2641 cggcggcgaa gcccaccacc agcaggcgcc cgcgccaggc gagcgagcgg aacgccggct
     2701 cgctgtaggg cccacccact gggtcgtaaa cgacgtccac tccgcttttt cctgttatcg
     2761 cctggatgcg ctcgcgcagg tcctcggtcg agtagttgat ggcttcatcg gcgccgtgac
     2821 tgcggcacac ggcgagcttg tcctcgctcg aggcgcaggc gatgacgcgc gcgccgagcg
     2881 ccttgccgat gtcgatcgca gccaggccga cgccgccggc ggcgcccagc accagcagcg
     2941 tttcgccggc gcgcagcgcg ccgcggtcgc gcagcgcgtg gtcggaagtc ccgtaggtga
     3001 ggagaaacgc cgcgccggtg gcgaaatcca tgccctgcgg cagcggcagc agccgcgcgg
     3061 cctcggtctt cacctcttct gcgaaggcgc cgtaagtggt gaaggcgatg accttgtcgc
     3121 ccggcttgaa gccctcgacg ccggcgccgg tttccttgac caccccggcg agctcgctgc
     3181 cgggagagaa cggcagcggc ggcttgaact ggtacttgtt ctggatgatg aggacatcgg
     3241 ggaagttgac gctcgccgcc ttgaccgaga gcaccacctc gcccggcccg gcgcgcggcg
     3301 agggcatatc ctccaccacc agcgaatcag gcgggccgaa ctgcttgcaa acaaccgctt
     3361 tcatcaacat agaatgccac aatgcaagag ctgatcctgc atcactaccc ggtttcgcct
     3421 ttcgccgaga agatccgcac catcctcggc tacaagaagc tcgcctggcg ctcggtcgag
     3481 atcccgatgg tcctgcccaa gcccgacgtg ctcgcgctca ccggcggcta tcggcgcacg
     3541 ccgctcctgc agatcggcgc cgacgtctac tgcgacacgg cgctgatcgc gcgcacgctc
     3601 gagcgcatcg cgcccgagcc gtcgatcttt ccgcagagcg aaacgctcgc ggtgcaggcc
     3661 gccgccttct tcgccgacaa cgtgctgttc aacatcgcgg tgcccatcgg cttccagccc
     3721 ggcggcatgc tcaagctgtt cctgcccgac gcgagctccg agttcctcga ccggttcgcc
     3781 cgcgaccgcg ccgccatgcg ccagggcggc acggtgcggc gcgggccgct cgccgaatgc
     3841 aaggccaacc tcgcgcacac gctggcgcgc gtcgaggcgc agctgcaggg gccgtttctg
     3901 ttcggcgcca ggccgacggt ggcggatttc tcgctctacc acgtgctctg gtcggtgtgg
     3961 aagccgccga tcgcgcggcc gctgctcgat ccgtttccga aaaccgtggc gctggtcgag
     4021 cgcatcgccg cgttcggcca cggccgcccg ctgccccttg gcagcaccga tgccctggag
     4081 atctcgaaga aggccaggcc cgcgccgatc gagaaacccc aagcggtgga aaccggcggc
     4141 gtcgcgctcg gcgagatggc cgaggtgatg ccggtggatt cagcgctcga tccggtgcgc
     4201 ggcgagctgg tgcattgcag cgtcgacgaa atcgtcctgc ggcgcagcga cccgcgcgcc
     4261 ggcacgatag cggtccactt cccacgtttc ggctaccaga tgagaccatg ccaagcctga
     4321 gcaccgacga cggcgtcaag ctgtactacg aggaggcggg cgccggcacg ccgatcgtct
     4381 tcgtccacga gttcgccggc gaccatcgca gctgggagcc gcagctgcgc cacttctcgc
     4441 gccgctaccg ctgcatcgcc tacaacgcgc gcggctaccc gccctcggag gtgccgcagg
     4501 acgtcgagcg ctactcgcag gcgcgctggc gcgacgacat ccgctgcgtg ctcgacgcgc
     4561 tgggcatcca gcgcgcccac gtcgtgggcc tgtcgatggg cgcgttcgcc tcgctgcact
     4621 tcggcatcac ctacggctac cgcgcgctgt cgatcacggt ggcgggcggc ggctatggcg
     4681 cccaccccgc ccagtaccgc aagttccagg aggacagccg caccaacgcc gcgttcatcc
     4741 agcgcgaggg aatggcgaag ttcgcggcga cctacggcca cgggccgacg cgcgtgcagt
     4801 tccagaacaa ggacccgcgc ggcttcgccg agtacatcgg ccagctcacc gagcactccg
     4861 cgctcggctc ggcgaacacc atgctcggct accagtcgcg gcgtccgtcg ctgtacgacc
     4921 tcaccgacga gatggcgcgc atcgaggtgc ccacgctcat catggcgggc gacgaggagg
     4981 agccctgcct cgaggtgtgc ctgctcatga agcgcgccat ccccaccgcc ggcctcgcgg
     5041 tcctgccgcg cagcgggcac ggcatcaatc tcgaggagcc ggcgctgttc aaccggttgc
     5101 tggaggattt cttccgccag gtggaagcgg gccgctgggg ccggcgcgac ccgcgcgcga
     5161 cggtgccttc cctctacggt ccgggaggca agccctgact tcttcatcct ccgagggcaa
     5221 gctgacggtg gaaaaacgcg gcgccgtcgg ctggatcgtg ttcgactcgc cggccaagcg
     5281 caacgcgatc aacggcgcca tgtggcgcgg catcgcgccg gccatggcgc gcttcgacgc
     5341 cgacccggaa gtgcgctgtg tggcatttcg tggttccggc agagaagcat tctctgccgg
     5401 agccgatatt tccgagttcg acacgatccg cgcgcagaac tcggccgtgt cggagtacga
     5461 cggcctgctc gaccaggtgc tgcacgccat ccaggactcg cgcaaggcct cggtcgcgat
     5521 gatccacggc ttctgcatgg gcggcggcct cgagatcgcg ctcgcctgcg acctgcgcta
     5581 ctgcggcgcc tcggcgcagt tcggcatccc ggcggcgaag ctcggcctcg cctacaacgt
     5641 cgaggggcac aggcggctgc tcgagaccgt cggccacgcc cgcgcgcgcg agatcatgtt
     5701 cctgggcggg cgctacatcg ccgccgacgc gctggcgatg ggactggtga accaggtcct
     5761 gcccgacgac gcgctggaga agcacaccgc cgaaatcctg cagaccctct cggacaatgc
     5821 gccgctcgcc atcgccaact cgaagaccat catcgaggaa tacgcgaagt cgcagggcgc
     5881 gccggacgcc gcggcgatgc agcgggcgat gcagcgctgc atcgacagcg aggactacaa
     5941 ggaggggcgg cgcgcgttca tggagaagcg caagccgcgc ttcacgggaa gatgaaaatc
     6001 cagggggtcc ggctgcaggc ggtggccgtg ccgataaagc gtccgctgca gacgagcatc
     6061 gcggcggtga c
//
LOCUS       DASYJA010000288         2547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2401887, whole genome shotgun sequence.
ACCESSION   DASYJA010000288 DASYJA010000000
VERSION     DASYJA010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2401887"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VGO02_06215"
     CDS             <1..777
                     /locus_tag="VGO02_06215"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEV7477315.1"
                     /translation="VDNRAGAAGAIAADALVKSTPDGYTLLLAESGTLIVPSMNPKAA
                     YDPLRQFAPVAGACSLPLAFVVAPGFPAANARELIAALKADPGKHSYASPGIGTLQHL
                     AFELFRRQAGVDAVHVPYKGASAMIPDLMSGQVEIAVISATVAIAQSKSGKIRTLAVT
                     SPQRMTNAPDLPALAETLPGFSAAPNVFVVAPAGTPPAIIQRLSTAVRSAVSSKDAEE
                     SLARLGATPWPLGAEELGAQIAREVKQWAAVVKDAGIRIE"
     gene            774..1928
                     /locus_tag="VGO02_06220"
     CDS             774..1928
                     /locus_tag="VGO02_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEV7477316.1"
                     /translation="MKIARVAATPLNVPIRLDAAGIEKQTSLSVCLVEVETDDGLTGH
                     GFTAITEEEVVGAAINEVAAGSLIGEDPLAHEKIWEKLYWLLSPRGQTGYASHAIAAI
                     DVALWDLKGKFFKQPVFRLLGGARSRVPVYTTFGFGAYSRDELAAAAKWWVGRGHRKL
                     KMVVGHHGLQRRDEPRPLDEVIAEDLRRVRAVRDAVGMEVDLHVDANCSLDSFHAARL
                     ARSLEELRISFFEEPVKENDIPALLSLRQFTKLPIAAGQNEGLAWRFRDLMVSRAVDI
                     VQPNVVISGGFTQCLRIAGMASAFNVPMANGGAWAHHNQHLHAGLANGGLVEYHYPAV
                     KVCEAIYGPLPAPVDGWLELPEKPGLGFSPDPLLVKELAKLPTSRGKGKA"
     gene            1931..>2547
                     /locus_tag="VGO02_06225"
     CDS             1931..>2547
                     /locus_tag="VGO02_06225"
                     /inference="COORDINATES: protein motif:HMM:NF015552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477317.1"
                     /translation="MRNLHIGMYGAVMGLAGLGLTARAAAPLFPGVFRAPAYFTELWV
                     ALGVLALLILAVLHLVELFASPKGVQADFTDPALMGFTGALPVGMTLVAGGLGPYAPG
                     FASGLWWAGCLLLSALQVCAIARWLAGQVPLDKLNAGWLILIVGGIVVPGPGISLGHA
                     EASHVFFGVSACAAVVLLPLLLARAALAAALPEALRPTWFILLVPP"
BASE COUNT          373 a          871 c          881 g          422 t
ORIGIN      
        1 gtcgacaacc gcgccggcgc cgccggcgcg atcgccgccg atgcgctggt gaaatcgacg
       61 cccgacggct acacgctgct gctcgccgag agcggcacgc tgatcgtgcc gagcatgaac
      121 ccgaaggccg cctatgatcc gctgcgccag ttcgcgccgg tggccggcgc ctgctcgctg
      181 ccgctcgctt tcgtcgtggc ccccggcttc cccgcggcca acgcgcggga gctgatcgcg
      241 gcgctgaagg ccgacccggg caagcacagc tacgcctctc cgggtatcgg cacgctgcag
      301 catctcgcct tcgagctgtt ccggcgccag gcaggggtgg acgcggtaca cgtgccctac
      361 aaaggcgcct cggcgatgat tccggacctg atgagcggcc aagtggagat cgccgtgatc
      421 agcgccactg tagcgatcgc gcagagcaaa tcgggcaaga tccgcaccct tgcggtaacc
      481 agcccgcagc gcatgaccaa cgccccggac ttgccggcgc tcgccgagac gctgccaggc
      541 ttcagcgccg cccccaacgt gttcgtggtc gcgccggcgg gaacgccgcc ggccatcatc
      601 cagcgcctat ccacggccgt gcgctcggcg gtgtcctcga aggacgcgga ggagagtctt
      661 gcccggctgg gcgcaacgcc atggccgctg ggcgccgagg agctcggcgc gcagatcgcg
      721 cgtgaggtga agcagtgggc cgcggtggtg aaggacgccg gcatcaggat cgaatgaaga
      781 tcgcccgcgt cgccgcgacg ccgctaaacg tgccgatacg gctcgatgct gccggcatcg
      841 agaagcagac ttccctgtcc gtctgcctgg tggaggtcga aaccgacgac ggcctgaccg
      901 gccacggctt caccgccatc accgaggagg aagtggtcgg tgcggccatc aacgaggtcg
      961 ccgccggcag cctgatcggc gaagacccgc tggcgcacga aaagatctgg gaaaagctct
     1021 actggctgct gagcccgcgc ggccagaccg gctatgcgag ccacgccatc gccgccatcg
     1081 acgtagcgct gtgggacttg aagggaaagt ttttcaagca acccgtgttc cgtttattgg
     1141 gcggagcgcg ttccagggtt ccggtctaca ccaccttcgg cttcggcgcg tacagccgcg
     1201 acgagctggc cgcggcggcg aagtggtggg tggggcgcgg ccatcgcaag ctcaagatgg
     1261 ttgtcgggca tcacggcctg cagcggcgcg atgagccgcg gccgctcgac gaggtgatcg
     1321 ccgaagacct gcgccgcgtg cgcgccgtgc gcgacgccgt cggcatggag gttgacctgc
     1381 acgtcgacgc gaactgcagc ctggacagct tccatgccgc gcgcctggcg cgctcgctgg
     1441 aggagctgcg gatcagcttt ttcgaggagc cggtcaagga aaacgacatt ccggcacttc
     1501 tttccctcag gcaatttacc aagctgccaa tcgccgcggg ccagaacgaa ggcctggcgt
     1561 ggcgcttccg cgacctgatg gtttcccggg ccgtcgatat cgtgcagccc aacgtagtga
     1621 tcagcggcgg cttcacgcag tgcctgcgca tcgccggcat ggcctcggcc ttcaatgtgc
     1681 cgatggccaa cggcggcgcc tgggcgcatc acaaccagca cctgcacgcc gggctcgcca
     1741 acggcggcct ggtcgagtac cactatcccg cggtgaaggt ctgcgaggcg atctacgggc
     1801 cgctgcccgc gccggtcgac ggctggctcg agctgccgga gaagcccgga ctcgggtttt
     1861 ctcccgatcc tctccttgtc aaagagctgg ccaagctgcc gacctcgcgc ggcaagggca
     1921 aggcttgacc gtgcggaacc tgcacatcgg catgtacggc gcggtgatgg gcctcgccgg
     1981 cctcggcctg accgcgcgcg cggcggcgcc gctcttcccg ggagtatttc gggcgccggc
     2041 ctatttcacc gagttgtggg ttgcgctggg tgtattggcg ctgctgatcc tcgcggttct
     2101 ccatctggtg gaattgtttg caagcccgaa gggcgtgcag gccgatttca ccgacccggc
     2161 cctgatgggc ttcaccggcg cgctgccagt cggaatgact ctggtggcgg gcgggctcgg
     2221 cccgtacgcg ccgggattcg cctccgggct gtggtgggcg ggctgcctgc tgctgagcgc
     2281 cttgcaggtc tgcgccatcg cccgctggct cgccgggcag gtgccgctcg acaagctgaa
     2341 cgcgggctgg ctgatcctga tcgtcggcgg catcgtggtg ccgggaccgg gcatctccct
     2401 cgggcacgcc gaagcttcgc atgtcttctt cggcgtttca gcctgcgctg ccgtggtgct
     2461 cctgccgctt ctgctggcgc gcgccgcgct tgccgcggct ctgccggagg cgctgcgccc
     2521 gacgtggttc atcctgctcg tgccgcc
//
LOCUS       DASYJA010000289         2868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2311486, whole genome shotgun sequence.
ACCESSION   DASYJA010000289 DASYJA010000000
VERSION     DASYJA010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2311486"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VGO02_06230"
     CDS             complement(<1..218)
                     /locus_tag="VGO02_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt efflux protein"
                     /protein_id="HEV7477318.1"
                     /translation="MNAPSRPSLLERLSALILREPGDREQLAQLLRSAYSRNLLDADA
                     MSIIEGALTVSEMQVRDIMIPRAQVDFI"
     gene            complement(215..664)
                     /gene="ybeY"
                     /locus_tag="VGO02_06235"
     CDS             complement(215..664)
                     /gene="ybeY"
                     /locus_tag="VGO02_06235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV7477319.1"
                     /translation="MPTTKRSRKNKVSVQYAVARRGVPAPAALRTWAGGASGVTLRIV
                     GASEGLRLNSLFRKKRKATNVLSFPEGNGRGDVVLCHPVIAREARAQGKTLAAHYAHL
                     VVHGILHLRGYDHEKKRDALRMERREIGILRRLGFSDPYAVKSPSLR"
     gene            complement(633..1571)
                     /locus_tag="VGO02_06240"
     CDS             complement(633..1571)
                     /locus_tag="VGO02_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEV7477320.1"
                     /translation="MPAKAVEVKLDPVDNQRLARLCGALDENLRQIESALDVSIARRG
                     ARFTVKGEHAGRAAEAIEHFYDRATGDLTAEDVQLGLTELSQNADKAEGPKLLTRRAD
                     LHGRTPHQLQYIENILAHDCTFGIGPAGTGKTYLAVACAVDALEREKVKRIVLVRPAV
                     EAGERLGFLPGDLAQKVDPYLRPLYDALYDLMGFEKTGKLLERGTIELAPLAYMRGRT
                     LNHSFIILDEAQNTTPEQMKMFLTRIGFGAKAVVNGDVTQIDLARGQKSGLIESRRIL
                     EGVRGIAFTEFGAQDVVRHPLVARIIDAYDKAKQEE"
     gene            complement(1579..>2868)
                     /gene="miaB"
                     /locus_tag="VGO02_06245"
     CDS             complement(1579..>2868)
                     /gene="miaB"
                     /locus_tag="VGO02_06245"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEV7477321.1"
                     /translation="CQMNEYDSAKIADLMRAAEGLEPAARPEEADLIVFNTCSVREKA
                     QEKVFADLGRVKHLKRANPKLMIAVGGCVASQEGAAIVERAPYVDVVFGPQTLHRLPQ
                     LLARRRASGDAQVDISFPEIEKFDHLPAAAVSGPSAFVSIMEGCSKYCSFCVVPYTRG
                     EEVSRPFEDILAELAGLAEQGVKEVTLLGQNVNAWRGAIDGDAADFAELLFFVNEIEG
                     IERIRYTTSHPREFTQRLVDAHAQLDKLAPHVHLPVQSGADRVLAAMKRGYTSLEYRS
                     IARRLKQARPGLSLTSDFIVGFPGESEADFAASLRLAAEMDFDGSFSFVYSPRPGTPA
                     AELPDGVSPAEKLARLQRLQAQLDLQYQKISESMVGGVERVLVESPSKKDQAELAGRT
                     ANNRVVNFPGGLELIGSFAEVRVTAALSHTLRGQIAK"
BASE COUNT          433 a          941 c          988 g          506 t
ORIGIN      
        1 tcgatgaagt ccacctgggc gcgcgggatc atgatgtcgc gcacctgcat ctcggagacg
       61 gtgagcgcgc cctcgatgat cgacatcgcg tcggcgtcga gcaggttgcg cgagtaggcc
      121 gagcgcagca gctgcgccag ctgctcgcgg tcgcccggct cgcgcaggat gagcgcagag
      181 aggcgctcga gcaggctggg ccgggacggg gcgttcatcg gagggagggg gattttaccg
      241 cgtaggggtc ggagaagccg aggcggcgca ggatgccgat ctcgcgccgc tccatgcgca
      301 gcgcatccct tttcttttca tggtcgtagc cgcgcaagtg aaggatgccg tgcaccacga
      361 gatgcgcata gtgcgcggcc agcgtcttgc cttgcgcacg cgcttcacgc gcgatcaccg
      421 ggtggcacag caccacgtcg ccgcggccgt tgccctccgg gaaggacagc acgttcgtcg
      481 ctttgcgctt ttttctgaag agtgagttca agcgcaggcc ttcgctcgcg ccgacgattc
      541 tcaaggtcac ccccgaagcg cctcctgccc aggtccgcag ggccgcaggg gcggggacgc
      601 cccgccgcgc gactgcgtac tgcaccgaaa ccttattctt cctgcttcgc tttgtcgtag
      661 gcatcgatga tgcgcgcgac cagcgggtgg cgcaccacgt cctgcgcgcc gaactcggtg
      721 aaggcgatgc cgcgcacgcc ctcgaggatg cggcgcgact cgatcaggcc gctcttctgg
      781 ccgcgagcca ggtcgatctg cgtgacgtcg ccgttgacca ccgccttggc gccgaagccg
      841 atgcgcgtga ggaacatctt catctgctcg ggggtggtgt tctgcgcctc gtccaggatg
      901 atgaaggaat ggttgagcgt gcgaccgcgc atgtaggcaa gaggcgccag ctcgatggtg
      961 ccgcgctcga ggagcttgcc ggtcttctcg aagcccatca ggtcgtagag ggcgtcgtac
     1021 agcggtcgca ggtacggatc gaccttctgc gccaggtccc cgggcaggaa ccccaggcgc
     1081 tcgcccgcct cgaccgccgg ccgcaccagc acgatgcgct tcaccttctc gcgctcgagc
     1141 gcgtccaccg cgcaagccac cgcgaggtag gtcttgccgg tgccggcggg gccgatgccg
     1201 aaagtgcagt cgtgcgccag gatgttctcg atgtactgca gctggtgcgg cgtgcggccg
     1261 tgcaggtcgg cgcgccgcgt gagcagtttg ggtccttcgg ccttatctgc gttttgtgag
     1321 agctcggtca gcccgagctg cacatcctcg gccgtcaaat cgcccgtggc gcgatcgtag
     1381 aaatgctcga tcgcttcggc ggcgcgcccg gcgtgctcgc ccttcacggt gaagcgcgcg
     1441 ccgcggcgcg cgatgctgac gtcgagcgcg ctctcgatct ggcgcaggtt ctcgtcgagc
     1501 gctccgcaca ggcgggcgag gcgctggttg tccaccgggt cgagcttcac ttcgacggcc
     1561 ttcgccggca tcaggctctc atttggctat ttggccgcgc agcgtgtgcg atagcgcggc
     1621 ggtgacacgc acttcggcga acgagccgat cagctccagg ccgccgggga agttcacgac
     1681 gcggttgttg gccgtgcggc ctgccagttc ggcttgatct ttttttgacg gcgattcgac
     1741 caggacgcgc tcgacgccgc ccaccatgga ttcgctgatt ttctggtatt gaaggtcaag
     1801 ttgggcctgc agccgctgca accgcgcaag cttttcagca ggagaaaccc cgtccggcaa
     1861 ctccgccgcc ggcgttcccg gccgcgggct gtagacgaac gagaacgagc cgtcgaaatc
     1921 catctcggcg gcgaggcgca ggctggcggc gaaatcggcc tcgctctcgc cggggaagcc
     1981 gacgatgaaa tccgaggtga ggctcaagcc ggggcgcgcc tgcttgaggc ggcgcgcgat
     2041 cgagcggtac tcgagcgagg tgtagccgcg cttcatcgcg gcgagcacgc ggtcggcgcc
     2101 cgactgcacc ggaaggtgca cgtgcggcgc gagcttgtcg agctgcgcgt gggcgtcgac
     2161 cagccgctgc gtgaactcgc gcggatgcga ggtggtgtag cggattcttt ctattccttc
     2221 aatttcattc acgaaaaaaa gaagttcggc gaagtccgcg gcgtcgccat cgatcgcgcc
     2281 gcgccaggcg ttgacgttct gcccgagcag cgtcacctcc ttcacgccct gctcggcgag
     2341 gccggcgagc tcggcgagga tgtcctcgaa ggggcgcgat acctcctcgc cgcgggtgta
     2401 gggcacgacg cagaagctgc agtacttgct gcagccttcc atgatcgaga cgaatgccga
     2461 ggggccgctg accgccgccg cgggcagatg gtcgaacttc tcgatctcgg ggaagctgat
     2521 gtcgacctgg gcatcgccgc tggcgcggcg gcgcgccagc agctgcggca ggcggtgcag
     2581 cgtctgcggc ccgaacacca cgtcgacgta gggcgcgcgc tcgacgatcg ccgcgccttc
     2641 ctggctcgcg acgcagccgc ccacggcgat catcagcttc gggttggcgc gcttgagatg
     2701 cttcacccgg ccaaggtcgg cgaacacctt ctcctgcgct ttctcgcgca ccgagcaggt
     2761 attgaagacg atcaggtcgg cttcctcggg ccgcgccgcg ggctcgagcc cctcggccgc
     2821 gcgcatcagg tcggcgatct tcgccgaatc gtactcgttc atctggca
//
LOCUS       DASYJA010000290         6665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2039617, whole genome shotgun sequence.
ACCESSION   DASYJA010000290 DASYJA010000000
VERSION     DASYJA010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6665
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2039617"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VGO02_06250"
     CDS             complement(<1..726)
                     /locus_tag="VGO02_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEV7477322.1"
                     /translation="MTPLAAGLVLFALLLVLLAGGVWIAIALGAVAWFGLQFFTSTPP
                     DVNLFQSFWGSSASWTLAALPLFIWMGEILYRTKLSEEMFEGLAPWLGWLPGRLMHVN
                     ILGCAIFGTVSGSSAATCATIAKVALPELKRRGYDEKTCLGSLAAAGTLGILLPPSII
                     MVVYAVAAEVSIIKVFLAGFIPGFVLTALFSGYIVVWALLNPGKTPPDEMKMSFKEKL
                     YKARHLVPCLVLIVFVVWVMVIGW"
     gene            complement(726..1259)
                     /locus_tag="VGO02_06255"
     CDS             complement(726..1259)
                     /locus_tag="VGO02_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEV7477323.1"
                     /translation="MRRFLDRLYTGSGVLAALCVFGIFALMLVQAFGREAGILVRGAD
                     DIVAWLCAAASFLALGHTFRHGELVRVGLLLERLGPRGRRAMELFALGLTALFVAYML
                     WAVGRFIYESWKFNEVAQGLIRVPIWIPQMAFLVGVIIFFIAVLDDLIAVLQNKKPAY
                     QAAEEERRASGDFSESV"
     gene            complement(1264..2226)
                     /locus_tag="VGO02_06260"
     CDS             complement(1264..2226)
                     /locus_tag="VGO02_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEV7477324.1"
                     /translation="MQKTLIAVAAFAVFSAHAQTKWDMPTPYSDGEFHTRNVALFVED
                     VKKATNGGFAVTVHANGSLLKHPDILRAVSTGQVSIGEFLLGQFGNEEPVFNADNVPF
                     LAAGYDDAWKLYQAQKPILEKKLEGRGMKLLYSVAWPGQGIYSKEPLKSVADLKGTKF
                     RTYSPMTARLAELLGASPTVVQVPDVPQMFATGGIQAMITSSATGTSTKAWEFVKNYY
                     DTSAFHPKNVVVVNARSLARLSAAEQKALADAATAAEKRGWEMSKTRTTEANDLLTKN
                     GMTIHQPDAAMKAAYAKVGDQILDEWLKVAGADGQAILKAYRGK"
     gene            complement(2256..3362)
                     /locus_tag="VGO02_06265"
     CDS             complement(2256..3362)
                     /locus_tag="VGO02_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7477325.1"
                     /translation="MEKLDAVVVGAGVVGLAVARALAMAGREVVVLEAEEAIGTHTSS
                     RNSEVIHAGIYYSRNSLKASSCVAGKELLYEYCVSHGLAHRRSGKLIVASHADQLEEL
                     QNIQKKAHANGVTDVVWMDCDQVLALEPELQCVAGLYSPSTGIIDSHALMLAYQGDAE
                     AHGAMLALKSSLQKAVLTNEGFVLHVDGSEPIQTRILVNSAGLRAPSVAKAVEGYRAA
                     LAPKELYAKGNYYTLTGKNPFTRLVYPVPEPGGLGVHVTLDMAGQARFGPDVEWVDRI
                     GQREDYAVDPRRADRFYAAIRRYWPGLPDGSLAPGYSGIRPKTAGPGEPAPDFEIQGP
                     RRHGVPGLVHLFGIESPGLTASLALAGMVLEELH"
     gene            complement(3349..4533)
                     /locus_tag="VGO02_06270"
     CDS             complement(3349..4533)
                     /locus_tag="VGO02_06270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEV7477326.1"
                     /translation="MNNKPTPASLTVLLGLLMALPALGTDLFVPSLPALAQSFAVSVS
                     AGQSALTTYFVGLAAGMLVWGPLSDRFGRRPVLLAGLATMFAASVAAALMDSIAGVAG
                     ARLAQGFAMSSGAVVVRSIVRDLHAHEQAARLLASITIVFSMVPIAAPVVGAQLAGRL
                     GWQAVFWGIAAAGAALLAATAAWLKETAPLPRRSIHPLALAASFASIGRDRRFFAPML
                     LALCTQVGLLAWVANSAFTLVRGLGISTVAYGWMFALVMLGQISGAWCSRHLVMRIGM
                     ARLMRAGVLLSLGAGATAAALAWAGVAHWLAVVLPFALFLFGTALVMPNATATALSPF
                     PGMAGTVSSLLGAICFAGGALMSTALGAFFDGTARPMASAAALAGLCAFLFERRLTRG
                     KA"
     gene            4548..>6665
                     /locus_tag="VGO02_06275"
     CDS             4548..>6665
                     /locus_tag="VGO02_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEV7477327.1"
                     /translation="MGWQFWIDRGGTFTDIVARRPDGSLLTHKLLSENPERYADAALA
                     GIRHLLGLQENSSIPGAAIEAVKMGTTVATNALLERKGERTALFITRGFRDALRIAYQ
                     NRPRIFDRRIVLPEMLYEEVFEVEERIGARGEVLTPLDEKDLLEKLKTCRTKGFHSVA
                     IVFMHGYRFPQHENLAFDIAKRLGFEQISVSHRVSPLMKLVGRGDTTVVDAYLSPILR
                     RYVERVAGELDGVRLLFMQSNGGLADARRFQGKDAILSGPAGGIVGAVRTAAAAGHER
                     IIGFDMGGTSTDVSHYAGELEREFETRVAGVRMRAPMMSIHTVAAGGGSILRFDGARY
                     RVGPESAAANPGPASYRRGGPLTVTDANVMLGKIQPKYFPRVFGADGKQALDEIAVRN
                     GFLRLKEQIKDHRTAEEIAAGFVQIAVGNMANAIKHISVQRGHDVTAYTLCCFGGAAG
                     QHACLVADALAMKRVFIHPLAGVLSAYGMGLADQVAIRQQAVEAGLDAELLGGIESRL
                     AHEARSELVSQGVPAGKISTSARVHLKYEGTDTALLVPLGTPAEMTGRFEAAYRKQFS
                     FLMPGKALIAEALSVEATGGGEVFEEKSPLGKTHDANPAETVRMFTGGGWHDTPVYRR
                     EELAPGQSIEGPAIIAEAHATTVVEPEWRANVTALNHLLLERAREKRKASAIGTSVDP
                     VMLEIFNNLYMSIAEQMGLRLQNT"
BASE COUNT         1232 a         2235 c         2212 g          986 t
ORIGIN      
        1 ccagccgatc accatcaccc acacgacgaa gacgatgagc acgaggcagg ggacgaggtg
       61 cctcgctttg tagagcttct ccttgaacga catcttcatt tcatccggcg gcgtcttgcc
      121 ggggttcagc agcgcccaga cgacgatgta gccggagaag agcgcggtca gcacaaagcc
      181 ggggatgaag cccgcgagga acaccttgat gatcgagacc tcggcggcca ccgcgtacac
      241 caccatgatg atcgagggcg gcagcaggat gccgagggtg ccggcggcgg cgagcgagcc
      301 gaggcaggtc ttctcgtcgt agccgcggcg cttgagctcg ggcagcgcca ccttcgcgat
      361 cgtcgcgcag gtggcggccg aggagcccga gacggtgccg aagatggcgc agccgaggat
      421 gttgacgtgc atcaggcgcc ccggcagcca gccgagccag ggcgcgaggc cctcgaacat
      481 ctcctccgac agcttggtgc ggtagaggat ctcgcccatc cagatgaaaa gcggcagcgc
      541 ggcgagcgtc cacgacgcgc tcgagcccca gaaggactgg aacagattca cgtcgggcgg
      601 cgtgctggtg aagaactgca ggccgaacca cgccaccgcg ccgagggcga tggcgatcca
      661 tacgccgccc gccaggagga cgagcaacag ggcaaaaaga accagacctg ctgccagcgg
      721 cgtcatcaga ccgactccga gaagtcgccg gaggcgcggc gctcctcttc agccgcctgg
      781 taggcaggtt ttttgttttg taaaactgca atcaaatcat ctagaacggc gatgaagaaa
      841 atgatcacgc cgaccagaaa cgccatctgc gggatccaga tcggcacgcg gatcaggccc
      901 tgcgccacct cgttgaactt ccagctctcg tagatgaagc gacccaccgc ccacagcatg
      961 taggcgacga acagcgccgt gaggccgagc gcgaacagct ccatcgcccg gcggccgcgc
     1021 gggccgaggc gctcgagcaa gagcccgacg cgcaccagct cgccgtgcct gaaggtgtgg
     1081 ccgagagcga ggaacgacgc agcggcgcac agccaggcga cgatgtcatc cgcgccacgc
     1141 accaggatgc cggcctcacg gccgaaggcc tggaccagca tcagcgcgaa gatgccgaaa
     1201 acacaaagag cggcgagaac gccgctcccc gtgtagaggc ggtccaggaa ccgccgcaac
     1261 ttcctactta ccgcggtagg ctttgaggat cgcctggccg tcggcgccgg cgactttcag
     1321 ccattcgtcc aggatctggt ctccgacctt ggcatacgct gctttcatgg ccgcgtcggg
     1381 ctgatgaatc gtcatgccgt tcttggtcag gagatcgttc gcttccgtgg tgcgcgtctt
     1441 ggacatctcc cagccgcgct tctccgctgc cgtcgcggcg tcagcgagcg ccttttgctc
     1501 cgccgccgac aggcgcgcga ggctgcgcgc gttcaccacc acgacgttct tcggatggaa
     1561 ggcgctggtg tcgtagtagt tcttgacgaa ctcccaggct ttggtggacg tgccggtggc
     1621 cgaggacgtg atcattgcct ggatgccgcc cgtagcgaac atctggggca cgtccggcac
     1681 ctgtacgacg gtcgggcttg cgccgagcag ctcggccagc cgcgcggtca tcgggctata
     1741 cgtacggaac ttggtgccct tgaggtccgc cacgctcttc agcggctcct tcgaatagat
     1801 gccctggccg ggccacgcca cggaatagag cagcttcatc ccgcggcctt cgagcttctt
     1861 ctcgaggatc ggtttctgcg cctggtacag cttccaggcg tcgtcgtacc ccgccgcgag
     1921 gaacggaaca ttgtcggcgt tgaataccgg ctcctcgtta ccgaactggc cgaggaggaa
     1981 ttcgccgatg ctcacctgac cggtcgatac cgcgcgcagg atgtccgggt gcttgagcag
     2041 cgacccattc gcgtgcacgg tgaccgcgaa cccgccgttc gtcgccttct tcacgtcttc
     2101 gacgaacagg gcgacgttgc gcgtatggaa ctcgccgtcg gagtacggcg tcggcatatc
     2161 ccacttggtc tgcgcgtgcg ccgagaacac agcaaacgca gcgacagcga tgagggtttt
     2221 ttgcattggt ttttcctgga aataattccc tgaagctaat gtaattcctc cagcaccatc
     2281 ccggcaaggg cgagcgaggc ggtgaggccg ggagactcga tgccgaagag atgcaccagc
     2341 ccggggacgc cgtggcggcg cgggccctga atctcgaaat ccggcgccgg ctcgcccggc
     2401 cccgcggtct tcggccggat gccggagtag cccggcgcga gcgagccgtc gggcaggccc
     2461 ggccagtagc gccggatcgc cgcgtagaac cgatcggcgc ggcgaggatc cactgcgtag
     2521 tcttccctct ggccgatgcg atcgacccat tcgacgtccg ggccgaagcg cgcctgcccg
     2581 gccatatcga gcgtgacatg cacgccaaga ccgccgggtt cgggcaccgg gtagaccagc
     2641 cgcgtgaagg gattcttccc ggtcagcgtg tagtagttgc cctttgcgta gagttccttc
     2701 ggcgcgagcg ccgcacggta gccctccacc gccttggcga cggaaggcgc gcgcaagccc
     2761 gcgctgttga ccaggatccg ggtttgaatc ggctcgcttc catcaacgtg aagcacgaag
     2821 ccttcattcg tcaaaactgc tttttgaaga gaactcttca aagccagcat cgcgccgtgc
     2881 gcctcggcat cgccctggta cgcgagcatc agcgcgtggc tgtcgatgat gccggtggag
     2941 ggcgagtaaa gccctgcgac gcattgcagc tcgggctcga gcgccaggac ctggtcgcaa
     3001 tccatccata cgacgtccgt gacgccattc gcgtgcgcct ttttctggat gttttgcagc
     3061 tcctcgagct gatctgcgtg agacgcgaca atcagcttgc cgctgcggcg gtgcgccagg
     3121 ccgtggctca cgcaatactc gtagagcagc tccttgccgg cgacgcagct ggatgctttc
     3181 agcgaatttc tagaataata aattcccgca tgaatcacct cggaattgcg cgagctggtg
     3241 tgcgtgccga tcgcctcttc ggcctcgagc accaccacct cgcggcccgc catggcgagg
     3301 gcgcgcgcca ccgccaggcc gacgacgccg gcgccgacga cgaccgcgtc aagcttttcc
     3361 acgcgtcagc ctccgttcga aaaggaaggc gcacaggccg gcgagcgcgg cggcgctggc
     3421 catcggtcgc gcggtgccgt cgaagaacgc gcccagcgcc gtgctcatca gcgccccgcc
     3481 ggcgaagcag atcgcgccga gcagcgacga cacggtgccc gccatgccgg gaaagggcga
     3541 gagcgccgtc gcggtggcgt tgggcatcac cagggcggtg ccgaacagga acagcgcgaa
     3601 aggcagcacg accgccagcc agtgcgcgac ccccgcccag gcaagcgccg cggcggtggc
     3661 gcccgcgccg agcgagagaa gcacgcctgc ccgcatcagc cgcgccatgc cgatgcgcat
     3721 caccaggtgg cgcgagcacc aggcgccgct gatctgcccg agcatcacca gcgcgaacat
     3781 ccagccgtag gcgacggtgg aaatgcccag cccgcgcacc agcgtgaagg cggagttcgc
     3841 cacccaggcg agcaggccga cctgcgtgca cagggcgagc agcatcggag cgaagaagcg
     3901 gcggtcccgc ccgatcgagg cgaagctcgc ggccagcgcc agcggatgga tcgagcggcg
     3961 cggcagcggc gcggtttcct tcagccaggc ggccgtggcg gcgagcagcg cggcgccggc
     4021 cgcggcgatc ccccagaaca ccgcctgcca gcccagacgg cccgcgagct gcgcgccgac
     4081 gaccggcgcg gcgatcggca ccatgctgaa gacgatggtg atgctcgcga gcagccgcgc
     4141 cgcctgctcg tgggcgtgca ggtcgcgcac gatcgagcgc accaccaccg cgccgctcga
     4201 catggcgaag ccctgcgcca ggcgcgcgcc ggccacgccg gcgatcgaat ccatcagcgc
     4261 ggccgccacc gaggcggcga acatcgtggc gaggcccgcc agcagcacgg gcctgcgccc
     4321 gaagcggtcg gaaagcggcc cccagacgag catgccggcg gcgagcccga cgaaataggt
     4381 ggtgagcgcg gactgcccgg cgctcaccga cactgcgaaa gactgcgcca gcgccggcag
     4441 cgacggcacg aacagatccg tgccgagcgc gggcagcgcc atcagcaatc ccaggagtac
     4501 cgtgagcgag gcgggggtcg gcttgttatt caagctagga ttctaagatg ggctggcaat
     4561 tctggatcga ccgcggcggc acgttcaccg acattgtcgc ccgccggccc gacggttcgc
     4621 tgctgacgca caagctgctg tcggagaacc ccgagcgcta cgccgacgct gcgcttgccg
     4681 gcatcaggca tcttttaggt ttgcaagaaa actcttccat cccgggggcg gcgatagaag
     4741 cggtcaagat gggcaccacg gtcgccacca atgcgctgct ggagcgcaaa ggcgagcgca
     4801 cggcgctatt catcacgcgc ggcttccgcg acgcgctgcg catcgcctac cagaaccggc
     4861 cgcgcatctt cgaccggcgc atcgtgctgc cggagatgct ctacgaggaa gtcttcgagg
     4921 tcgaggagcg catcggcgcc agaggtgagg tcctcacgcc tctcgacgaa aaagacctgc
     4981 ttgaaaagct gaaaacctgc cgcaccaagg gctttcactc cgtcgccatc gtgttcatgc
     5041 acggctaccg gttcccgcag cacgagaacc tggccttcga catagcgaaa cgcctcggct
     5101 tcgagcagat ctcggtttcg caccgcgtca gcccgctgat gaagctggtg gggcgcggcg
     5161 acacgacggt ggtcgatgcc tacctgtcgc cgatcctgcg ccgctacgtg gagcgggtcg
     5221 ccggcgagct cgacggcgtg cggctgctgt tcatgcagtc gaacggcggc ctcgccgacg
     5281 cgcgccgctt ccagggcaag gacgcgatcc tctccgggcc ggcgggcggc atcgtcggcg
     5341 ccgtgcgcac cgcggcggcc gccgggcacg agcgcatcat cggcttcgac atgggcggca
     5401 cctcgaccga cgtctcgcac tacgccggtg agctcgagcg cgagttcgag acgcgcgtcg
     5461 ccggcgtgcg catgcgcgcg ccgatgatga gcatccacac ggtggcggcg ggcggcggct
     5521 cgatcctgcg cttcgacggc gcgcgctaca gagtgggtcc cgagtccgcc gcagcgaatc
     5581 cggggccggc ttcctaccgg cgcggcgggc cgctcacggt caccgacgcc aacgtcatgc
     5641 tcggcaagat ccagccgaag tattttccca gggtattcgg cgcagacggc aagcaggccc
     5701 tggacgaaat cgcagttcga aatggattcc ttcgactaaa agagcaaata aaagaccacc
     5761 gaaccgccga agagatcgcc gcaggcttcg tgcagatagc cgtgggcaac atggcgaacg
     5821 cgatcaagca catctcggtg cagcgcgggc acgacgtcac cgcctacacg ctgtgctgct
     5881 tcggcggcgc cgccggccag cacgcgtgcc tggtggccga tgccctcgcc atgaagcgcg
     5941 tcttcatcca ccccctcgcc ggcgtgctct cggcctacgg catggggctc gccgaccagg
     6001 tcgccatccg ccagcaggcg gtcgaagcag gcctggatgc cgagctcctg gggggaatcg
     6061 aatcgaggct ggcgcacgag gcgcgcagcg agctcgtttc ccagggcgtc ccggcaggaa
     6121 agatcagcac ctcggccagg gtgcacctga agtacgaggg caccgacacc gcgctgctgg
     6181 tgcccctcgg cacgccggcc gagatgaccg ggcgcttcga ggccgcgtac cgcaagcagt
     6241 tctccttcct gatgcccggc aaggcgctca tcgccgaagc gttgtcggtg gaagccaccg
     6301 gcggcgggga agtttttgaa gagaagagtc ccctgggaaa aacacacgat gccaaccccg
     6361 ccgagacggt gcgaatgttc accggcggcg gttggcacga cacgccggtc taccggcgag
     6421 aggagctcgc gcccgggcag tcgatcgaag gaccggcgat catcgccgag gcgcacgcca
     6481 ccaccgtggt cgagccggag tggcgcgcga acgtgactgc gctgaatcac ctcctgctgg
     6541 aaagagccag ggaaaaaaga aaagcgagcg caatcggcac ttcggtcgac ccggtgatgc
     6601 tggaaatctt caacaacctc tacatgtcga tcgccgagca gatggggctg cgcctgcaga
     6661 acacc
//
LOCUS       DASYJA010000291         9943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1496130, whole genome shotgun sequence.
ACCESSION   DASYJA010000291 DASYJA010000000
VERSION     DASYJA010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9943
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496130"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VGO02_06280"
     CDS             complement(<1..511)
                     /locus_tag="VGO02_06280"
                     /inference="COORDINATES: protein motif:HMM:NF020331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEV7477328.1"
                     /translation="MRASAALLCAAALAACSSTEPMSDWERRNPEHLGTAQTERLPAP
                     PAMPRKENLIEFYVGPTATFHYFIDATSLSVFYKQKEVRYVLVARSPSGVENVSFEAI
                     RCPDTYRIFAVGDGGKWSERGGNWQPIQPRTGVGWPSVLARDYFCPHRDTLQSAAEGI
                     DALRQGGHPL"
     gene            complement(508..1083)
                     /locus_tag="VGO02_06285"
     CDS             complement(508..1083)
                     /locus_tag="VGO02_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HEV7477329.1"
                     /translation="MIKREDLLSLEMYARRRDQLRAEVIAHKKSRTVYLGEHLTLLFE
                     DQRTILYQVQEMLRIERIFEDEGIQGELDAYNPLVPDGGNWKATMLLEYPDAEERRRM
                     LGRLKGIEDRVWVQVGALARVYAIADEDLERENEEKTSAVHFLRFELPAEAREALARG
                     APLSMGVEHPDYAASMAVVGEIRDSLAGDLA"
     gene            complement(1160..1741)
                     /locus_tag="VGO02_06290"
     CDS             complement(1160..1741)
                     /locus_tag="VGO02_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV7477330.1"
                     /translation="MERDYQPLPIHELGTGAGFHRPTQSQAARVGLESPALLVMTDLT
                     RASPATIRPQAPLAGANQFMITRGVRLLLVVDDHEKVLGVITATDLVGERPIQVAVAR
                     GLRRDELAVADVMTEAAQVDMLSLADVESARVGHVLETLRQSGRQHALVVDAGGLVRG
                     IFSVSQIARQLGVALPAGGEVARTFSQIEAAIR"
     gene            complement(1794..2279)
                     /locus_tag="VGO02_06295"
                     /pseudo
     CDS             complement(1794..2279)
                     /locus_tag="VGO02_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523816.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
     gene            2367..4085
                     /locus_tag="VGO02_06300"
     CDS             2367..4085
                     /locus_tag="VGO02_06300"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV7477331.1"
                     /translation="MKTSKFFIATQKEVPAEAEIVSHRLMLRAGLIRRLAAGIYTWLP
                     LGLRVVRKVEAVVREEMNRAGAVELAMPVVQPGELWQESGRWQAYGPELLRLQDRHER
                     DFVMQPTSEEAITDLARAELRSYRRLPAHFYQIQTKFRDERRPRFGVMRGREFVMKDG
                     YSFHAGYDDLQREYRVMHDTYTRIFSRLGLKFRAVAADTGAIGGTGSHEFHVLADSGE
                     DAIAFSPDSDYAANVELAEAVFPVEKRKPPSEQMNKVPTPGKKTCEEVAELLKIPLQR
                     TVKSVVLAVDGEKAAKLFLLLIRGDHQLNEIKVSKLPDLAKFRFASESEIQRNFKSPA
                     GYLGPVGLKGVKIIADRTVAAMSDFVCGANEADFHLAGLNWGRDLAEPEVADIRNVVA
                     GDPSPDGKGRLEIVRGIEVGHIFQLRTKYSEAMKATFLDQAGKPQHFEMGCYGIGVTR
                     IVGAAIEQNHDQNGIVFPAPIAPFAVCIVPIGYGKSAAVRAAADKLYGELAAAGVDVL
                     LDDRDERPGVLFADMDLIGIPHRVVLGERGLAAGNAEYKGRRDDKPQDVPLAALAGLL
                     KEKLKG"
     gene            4085..4675
                     /locus_tag="VGO02_06305"
     CDS             4085..4675
                     /locus_tag="VGO02_06305"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEV7477332.1"
                     /translation="MKRLLALLAWLPLQALAGNQIYEPLADSMRQRLSTQVSDRAAPS
                     LHFRSAEDGSRWLATMEKRLESRMPDRKQRLELLRTVHYEALRAKLDPQLVLGVIEVE
                     SAFRKYAVSKAGARGYMQVMPFWIKQIGQPKHNLFHMRTNLAYGCAILRHYVDMEDGD
                     YFRALGRYNGSLGYSQYPRSVLVAWKIRWKYDGPTA"
     gene            4636..5640
                     /locus_tag="VGO02_06310"
     CDS             4636..5640
                     /locus_tag="VGO02_06310"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEV7477333.1"
                     /translation="MEDPLEVRRADRMIGALLDRRPRLANWIYGFTPPERGVVVLVHR
                     RVYIVPARLGWMFGATLALLLVGSINYAIALGFALTFLLAGLGLAGMVHTARNLARIA
                     VSTGRAEPVFAGEAAQFRVYLDGRAPHDRPAILARHVGSGSQMVVDVPGGGAAEVVLA
                     VPATRRGWLPLGRVMLETRFPLALFRAWSYVEPDARCLVYPKPERSPLPPSSAEADAG
                     GVRAPTPGNDDFAGLRGYQPADSPRHVAWKAVARSDELLTKQFTGEAAAELWLDWRLL
                     PAAGGLEQRLSLLAGWVLAAERRGVRYGLRLPGVEIPPGRGDSHSGAALQALALYRQP
                     "
     gene            5637..7604
                     /locus_tag="VGO02_06315"
     CDS             5637..7604
                     /locus_tag="VGO02_06315"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEV7477334.1"
                     /translation="MIAVPHPLAPGARLVGARDLAWLIGSLVLVAAPHALRAPWWLTV
                     LTLCLFAWRFWYALNGAPLPSRWLVIGVAAVAMLGVWMEYRTLFGRQPGIILLVLFAG
                     LKLLETRTHRDAALAAFLGYFLIITNFLYTQSIPTAALMCAALFALTATLVGFSAPRR
                     SLKANLRSAWLLLAHAAPAALVLFLLFPRVQGPLWGLPQDAYAGMTGLSESMSPGNLA
                     SLAQSDAIAFRAEFRGQTPPHAQRYWRGPVLWDFDGRTWTMIPVLLRDFTPPRGGRPY
                     PYDIVLEPHNRPWLFALESAASLPQGARMTRDGQILAAGPVRSRMRYEITSVVTPDAD
                     AQEDARLLSRALRLPPDSNPRAAALAAEWRAASSGDAEILARAIAFLRAGRYSYTLEP
                     ATLGAQPVDDFLFNTREGFCEHFSSAFVFLLRAAGVPARVVTGYQGGELNSVDSIITV
                     RQSDAHAWAEVFFAGRGWVRVDPTAAAVPGRVETGMARAVPQTQALPLMMREQLEWLK
                     GARDRWEALAHQWNVWVLGYNPERQRDLMNLVGMRDADWRSMTATLFTVLGLMTLVLL
                     AWSLGRLARPDPVQRAWRSFCRKLAAHGVERALHEGPRDYASRAARALPGSRRAILRI
                     GAQYIRLRYGAHASRPGVSRLRRMVQELHVA"
     gene            7601..8605
                     /gene="mltB"
                     /locus_tag="VGO02_06320"
     CDS             7601..8605
                     /gene="mltB"
                     /locus_tag="VGO02_06320"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEV7477335.1"
                     /translation="MRFLVILFCALAVSSAFAAPPAKPPAQASYAKRPEVKAFIRDMV
                     QRHGFVERELVFMFSRVKREDPVLQFIRPPDKGPSWQEYREIFVTEKRIALGAEFWRL
                     HSAQLERARREYGVPPEYVLAIIGIETYYGRNMGRWRVVDALTTLAFDYPPRSAYFRN
                     ELEQYLLLSRDLGFDVFSVRGSYAGAIGIPQFMPGSQRRFAVDFDGDGVVDLRRNPAD
                     AIGSVANFLRQHGWQPGGAVMFPARVEGEAWRAYAGIEPKHRLADLKRAGARFDAPAE
                     LDGALGALVELATPERASEYRVGLNNFYVLTRYNRSALYATAVSELAAALRKAQEAGK
                     "
     gene            complement(8587..9477)
                     /locus_tag="VGO02_06325"
     CDS             complement(8587..9477)
                     /locus_tag="VGO02_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEV7477336.1"
                     /translation="MNRVRLAAVAVMALALGAAACSTYRGPAPVEERARASKAASKPA
                     AAAAQAAKPAAAPATDGFYTVKQGDTLYSIALDNGADYREVAQWNALDDPTKLRIGQV
                     LRVKPEPRAPDARPGVVVGSARGAGRVESRSIDSAPPASAEGAAKTEPKALRLPYSKE
                     NAAMAAREEPKAEGEGVDFIWPVKGKVLAGFAEPRSKGIDIDGKLGDPVLAAAAGRVT
                     YIGTGIPGMGKLVVIKHDNGFITVYAHNRDILVKEQQSVTRGQKIAELGNTDADRPKL
                     HFQIRKGSAAVDPMRYLPAS"
     gene            complement(9474..>9943)
                     /locus_tag="VGO02_06330"
     CDS             complement(9474..>9943)
                     /locus_tag="VGO02_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEV7477337.1"
                     /translation="ISQPYIVARMIECLIANEKSPGKVLEVGTGCGYQAAVLAQIFSE
                     VYSIERIKGLHERARANLLGLKLSNLRLVHGDGYAGLEKAAPFQAIVVAAAARSVPEA
                     LLRQLAPGGRMVLPLRDGANGAQRLVLVERSGRGFVETELDTVRFVPMEAGKA"
BASE COUNT         1390 a         3601 c         3414 g         1538 t
ORIGIN      
        1 ccaaggggtg tccgccctgg cgcaaagcgt cgataccttc agccgcgctc tgcagcgtgt
       61 cgcggtgcgg gcagaaatag tcgcgcgcaa gcaccgaggg ccagcccacc ccggtgcgcg
      121 gctggatcgg ctgccaattg ccaccccgct cgctccactt gccgccgtcg ccgacggcaa
      181 agatgcggta cgtgtcgggg cagcggatcg cctcgaagct gacgttctcg acgccgctgg
      241 ggctgcgcgc gacgagcacg tagcgcacct ctttctgctt gtagaacacg ctcaggctgg
      301 tggcgtcgat gaagtaatgg aaggtggcag tcgggccgac gtagaactcg atcaggtttt
      361 ccttgcgcgg catggccggc ggcgcgggca gccgctcggt ctgcgccgtg cccaggtgct
      421 ccgggttgcg gcgctcccag tcgctcatcg gctcggtcga gctgcaggcg gcgagcgccg
      481 ccgcgcagag gagcgccgcc gaggccctca agcgagatcg cccgcgaggg agtcgcggat
      541 ctccccgacc accgccatgc ttgccgcgta gtccgggtgc tcgacgccca tgctgagcgg
      601 cgcgccgcgc gcgagcgcct cgcgcgcttc ggccggcagc tcgaaacgca ggaaatgcac
      661 cgcggaggtc ttttcctcgt tctcgcgctc gaggtcctcg tcggcgatgg cgtagacgcg
      721 cgcgagcgcg ccgacctgga cccagacgcg gtcctcgatg cccttcagcc ggccgagcat
      781 gcggcggcgc tcctcggcgt cggggtattc gagcagcatg gtcgccttcc agttgccgcc
      841 gtccgggacg agcggattgt aggcgtcgag ctcgccctgg atgccctcgt cctcgaagat
      901 gcgctcgatg cgcagcatct cctgcacctg gtagaggatg gtgcgctggt cctcgaacag
      961 cagcgtgagg tgctcgccga ggtacacggt gcggctcttc ttgtgggcga tcacctcggc
     1021 gcgcagctgg tcgcgccggc gggcgtacat ctcgagcgac aacaggtcct cccgcttgat
     1081 catgccgcta ggctaagccg ccccttggga tagatcaaat ggaaaaaccc aattcgtccg
     1141 attgcctgcc gctatccggt cagcggatcg ccgcctcgat ctgggagaag gtgcgcgcga
     1201 cctcgccgcc ggcgggcagc gccacgccga gctgcctggc gatctgcgag acggaaaaga
     1261 tgccgcgcac caggccgccg gcgtcgacca cgagcgcgtg ctgccgcccg gactggcgca
     1321 gcgtttcgag cacatggccg acgcgcgcgc tctccacgtc ggcgaggctc agcatgtcga
     1381 cctgcgccgc ctcggtcatc acgtcggcga cagccagctc gtcgcgccgc aggccgcgcg
     1441 cgaccgccac ctgtatcggg cgctcgccga ccaggtcggt ggccgtgatc acgccgagca
     1501 ccttctcgtg gtcgtcgacc accagcagca ggcgcacgcc gcgcgtgatc atgaactggt
     1561 tggcgccggc aagcggcgcc tgcggccgga tggtagccgg gctggcgcgc gtcaagtcgg
     1621 tcatcaccag cagcgccggc gattcgagac ccacacgcgc cgcctgcgac tgcgtcgggc
     1681 ggtggaaacc ggcgccggtg ccaagctcgt ggatcggcag cggctggtag tcgcgttcca
     1741 tcggattgcc ttcagctatt ggccgccgag cgcggcgcgg cgcgcaggtg ggcgcggccg
     1801 ccgtcctgca ggtagtggtg cagctcgagc agcgcctgcc ggtacacgtc gcgcttgaac
     1861 tcgatcaccg cctcgagcgg cacccagtag tcgttccagc gccaggcatc gaactcgggg
     1921 cggcagctgg cgcgcagctt gacgtcgcag tcgcgtccgg tcatgcgcag caggtaccag
     1981 atctgcttct gcccgcgata gtccgcggcg ccctcgcgcg cgcgccgcgc ccagcgctcg
     2041 ggcacctcgt agcgcagcca ttcgcgcgtg cggccgagca cgcgcacgtg ggcgcgctgc
     2101 aggccgacct cttcctcgag ctcgcggtac atcgcctgcg acggggtctc gccgtgcttg
     2161 atgccgccct gcgggaactg ccaggcgtgc tgattgatgc gcttgcccca gaacacctcg
     2221 ttcctctggt tggcaaggat gatgccgacg ttcggacggt agccttcctt gtcgagcacc
     2281 gcaggccgcc tcaagttagc ctaaagtaga atgcgcgatc cgatttttcc acaggcccca
     2341 gccaaacaca acggcggggc gctcgaatga aaacctcgaa gttcttcatc gcgacgcaga
     2401 aggaagtccc ggccgaggct gaaatcgtca gccaccggct gatgctgcgc gccgggctca
     2461 tccggcgcct cgccgccggc atctacacct ggctgccgct cggcctgcgc gtggtgcgca
     2521 aggtcgaggc ggtggtgcgc gaggagatga accgcgccgg cgccgtcgag ctggcgatgc
     2581 cggtggtgca gcccggcgag ctgtggcagg agtcgggccg ctggcaggcc tacggcccgg
     2641 agctgctgcg cttgcaggac cgccacgagc gcgatttcgt gatgcagccg acctccgagg
     2701 aggcgatcac cgacctggcg cgcgccgagc tgaggagcta ccggcgcctg ccggcgcact
     2761 tctaccagat ccagaccaag ttccgcgacg agcgccggcc gcgcttcggc gtgatgcgcg
     2821 gccgcgagtt cgtgatgaag gacggctact ccttccatgc cggctacgac gacctgcagc
     2881 gcgagtaccg cgtgatgcac gacacctaca cgcgcatctt cagccgcctc ggcctgaagt
     2941 tccgcgccgt ggccgccgac accggcgcca tcggcggcac cggctcgcac gagttccacg
     3001 tcctcgccga ctcgggcgag gacgcgatcg ccttctcgcc cgactccgac tacgcggcga
     3061 acgtggagct cgcagaagca gtttttccag ttgaaaaacg aaaaccgcca agcgaacaga
     3121 tgaataaagt cccgactccc ggcaagaaga cctgcgagga ggtggccgag ctgctcaaga
     3181 tccccctgca aaggacggtg aaatcggtgg tgctggcggt ggacggcgag aaggcagcga
     3241 agctgttcct gctgctgatc cgcggcgacc accagctgaa cgagatcaag gtctccaagc
     3301 tgcccgacct ggcgaagttc cgtttcgcca gcgagtccga gatccagagg aacttcaagt
     3361 cgccggccgg ctatctcggg ccggtggggc tgaagggcgt gaagatcatc gccgatcgca
     3421 ccgtcgcggc gatgagcgac ttcgtgtgcg gcgcgaatga ggccgatttt cacctcgccg
     3481 gcctcaactg gggccgcgac ctggccgagc ccgaggtcgc cgacatccgc aacgtcgtcg
     3541 ccggcgaccc gagccccgac ggcaagggca ggctcgagat cgtgcgcggc atcgaggtcg
     3601 gccacatctt ccagctgcgc accaagtact cggaggcgat gaaggccacc ttcctcgacc
     3661 aggccggcaa gccgcagcac ttcgagatgg gctgctacgg catcggcgtc acgcgcatcg
     3721 tcggcgccgc catcgagcag aaccacgatc agaacggcat cgtgttcccc gcgccgatcg
     3781 ctccgttcgc ggtgtgcatc gtgccgatcg gctacggcaa gagcgcggcg gtgcgcgccg
     3841 ccgccgacaa gctgtacggc gagctcgccg cggccggcgt cgacgtgctg ctcgacgacc
     3901 gggacgagcg ccccggggtg ctgttcgccg acatggacct gatcggcatc ccgcaccggg
     3961 tggtgctcgg cgagcgcggc ctcgccgccg gcaacgccga atacaagggg cgccgcgacg
     4021 acaagccgca ggacgtgccg ttggcagcgc ttgccggcct cttaaaagaa aaattaaaag
     4081 gttgatgaag cgtctgctcg cactcctcgc gtggttgccg ctgcaggcgc tggccggtaa
     4141 tcagatctac gagccgctcg ccgacagcat gcggcagcgc ctctccacgc aggtctcaga
     4201 ccgggccgcg ccgagcctgc acttccgctc cgccgaggac ggcagccgct ggctcgccac
     4261 catggaaaag cgcctcgagt cgcgcatgcc cgaccgcaag cagcgcctcg agctgctgcg
     4321 cacggtgcac tacgaggcgc tgcgcgccaa gctcgacccg cagctggtgc tgggggtgat
     4381 cgaggtggag agcgcgttcc gcaagtacgc cgtatccaag gccggcgcgc gcggctacat
     4441 gcaggtgatg ccgttctgga tcaagcagat cggccagccc aagcacaatc tcttccacat
     4501 gcgcaccaac ctggcgtacg gctgcgccat cctgcggcac tatgtcgaca tggaggacgg
     4561 cgactacttt cgcgcgctcg gccgctacaa cggcagcctc ggctattcgc agtatccgcg
     4621 ctcggtcctg gtggcgtgga agatccgctg gaagtacgac gggccgaccg catgatcggc
     4681 gccctcctcg accgccggcc gcggcttgcc aactggatct acggcttcac gccgccggag
     4741 cgcggcgtgg tggtgctggt gcaccggcgc gtgtacatcg tgccggcacg gctggggtgg
     4801 atgttcggcg ccaccctcgc gctgctgctg gtcggctcga tcaactacgc catcgcgctg
     4861 ggcttcgcgc tcaccttcct gctcgccggc ctgggcctcg ccggcatggt gcacaccgcg
     4921 cgcaacctgg cgcgcatcgc ggtgagcacc gggcgcgccg agccggtatt cgccggcgaa
     4981 gcggcgcaat tccgcgtcta cctcgacggc cgcgcgccgc acgaccgccc cgcgatcctg
     5041 gcgcgccatg tcggctcggg ctcgcagatg gtggtggacg tgccgggcgg cggcgccgcc
     5101 gaagtagtgc tcgccgtgcc cgcgacgcgc cgcggctggc tgccgctcgg ccgcgtcatg
     5161 ctcgagacgc gctttccgct cgccctgttc cgtgcctgga gctacgtcga gcccgacgcg
     5221 cgctgcctgg tctatcccaa gccggaacgc tcgccactgc cgccgtcgag cgccgaagcc
     5281 gatgccggcg gcgtgcgcgc tcccacgccc ggcaacgacg atttcgccgg cctgcgcggc
     5341 taccagccgg cggactcgcc gcgccacgtg gcgtggaagg cggtggcgcg cagcgacgag
     5401 ctgctcacca agcagttcac cggcgaggcg gcggccgagc tgtggctcga ctggcgcctg
     5461 ctgcccgccg ccggcggcct cgagcagcgc ctgtcgctgc tcgccggctg ggtgctcgcc
     5521 gccgagcggc gcggcgtgcg ctacggcctg cggctgccgg gggtcgagat cccgcccggc
     5581 cgcggcgaca gccacagcgg cgcggcgctg caggcactcg cgctgtacag gcagccgtga
     5641 tcgcggttcc acacccgctc gctcccggcg cacgcctggt cggcgcgcgc gacctcgcct
     5701 ggctgatcgg ctcgctcgtg ctggtcgccg cgccgcacgc gctgcgcgcg ccctggtggc
     5761 tcacggtgct cacgctgtgc ctgttcgcgt ggcgcttctg gtacgcgctc aacggcgcgc
     5821 cgctgccttc gcgctggctg gtgatcggcg tcgccgcggt ggcgatgctc ggcgtctgga
     5881 tggaataccg cacgcttttc ggccgccagc ccggcatcat cctgctggtg ctgttcgccg
     5941 gcctcaagct gctcgagacg cgcacgcacc gcgacgcggc gcttgccgcg ttcctcggct
     6001 acttcctcat catcacgaac ttcctctaca cccagtccat cccgaccgcc gcgctgatgt
     6061 gcgcggcgct gttcgcgctc accgccacgc tggtcggatt cagcgcgccg cgccgcagcc
     6121 tcaaggccaa cctgcgcagc gcgtggctgc tgctcgcgca cgccgcgccg gcggcgctgg
     6181 tcctgttcct gctcttcccg cgcgtgcagg gaccgctctg ggggttgccg caggacgcct
     6241 acgccgggat gaccggcctg tcggagagca tgagcccggg caacctcgcc agcctggcgc
     6301 aatccgacgc catcgccttt cgcgccgagt tccgcggcca gacgccgccg cacgcccagc
     6361 gctactggcg cgggccggtg ctgtgggact tcgacggccg cacctggacc atgatcccgg
     6421 tcctgctgcg cgacttcacg ccgccgcgcg gcggccggcc ttacccctac gacatcgtgc
     6481 tcgagccgca caaccgcccg tggctgttcg cgctggaaag cgcggccagc ctgccgcaag
     6541 gcgcgcgcat gacgcgcgac ggccagattc tcgccgccgg cccggtgcgc tcgcggatgc
     6601 gctacgagat cacttccgtg gtgacgccgg atgccgatgc ccaggaggac gcgcgcctgc
     6661 tgtcgcgcgc gctgcgcctg ccgccggatt cgaacccgcg cgccgcggcg ctcgccgccg
     6721 agtggcgcgc ggcctcgtcg ggcgatgccg agatcctcgc gcgcgccatc gccttcctgc
     6781 gcgccgggcg ctacagctat accctcgagc cggccaccct cggcgcgcag ccggtcgacg
     6841 acttcctgtt caacaccagg gaagggttct gcgagcactt ctcctccgcc ttcgtcttcc
     6901 tgctgcgcgc agccggcgtg ccagcgcgcg tggtcaccgg ctaccagggc ggcgagctca
     6961 attcggtcga cagcatcatc accgtgcgcc agtccgacgc gcacgcctgg gccgaggtgt
     7021 tcttcgccgg ccgcggctgg gtgcgcgtcg acccgaccgc ggccgcggtg cccgggcgcg
     7081 tcgagacggg aatggcgcgc gccgtgccgc agacgcaggc gctgcccctg atgatgcgcg
     7141 agcagctgga atggctgaag ggcgcgcgcg accgctggga ggcgctggcg caccagtgga
     7201 acgtctgggt gctcggctac aacccggagc gccagcgcga cctgatgaac ctggtcggca
     7261 tgcgcgacgc cgactggcgc agcatgacgg cgaccctgtt caccgtgctc ggcctgatga
     7321 cgctggtgct gctcgcctgg tcgctcggcc gcctggcgcg gcccgatccg gtgcagaggg
     7381 cgtggcgctc gttctgccgc aagctcgcgg cgcacggcgt ggagcgcgcg ctgcacgagg
     7441 ggccgcgcga ctacgcctcg cgcgcggcgc gcgcgctccc cggctcgcgc cgcgccatcc
     7501 tgcgcatcgg cgcgcagtac atccgcctgc gctatggcgc gcacgccagc cggcccggcg
     7561 tatcgcggct gcggcgcatg gtgcaggagc ttcacgtcgc atgaggtttc ttgtcatcct
     7621 gttttgcgcc ctcgcggtat cgagcgcgtt cgccgcgccg cccgccaagc cgccggcgca
     7681 ggcgtcctac gccaagcggc ccgaggtcaa ggccttcatc cgcgacatgg tgcagcgcca
     7741 cggcttcgtc gagcgcgagc tggtgttcat gttctcgcgc gtcaagcgcg aggacccggt
     7801 gctgcagttc atccgcccgc cggacaaggg cccctcatgg caggaatacc gcgagatctt
     7861 cgtcaccgag aagcgcatcg cgctcggcgc cgagttctgg cgcctgcaca gcgcccagct
     7921 ggagcgcgcg cggcgcgagt acggcgtgcc gccggagtac gtcctcgcca tcatcggcat
     7981 cgagacctac tacggccgca acatggggcg ctggcgcgtg gtcgacgccc ttaccacgct
     8041 cgccttcgac tacccgccgc gctccgccta tttccgcaac gagctggagc agtatctgct
     8101 gctctcccgc gacctcggtt tcgacgtctt ttcggtgcgc ggctcgtatg ccggcgccat
     8161 cggcatcccg cagttcatgc cggggagcca gcggcgcttc gcggtggatt tcgacggcga
     8221 cggcgtggtc gacctccggc gcaacccggc cgatgccatc ggcagcgtgg ccaacttcct
     8281 caggcagcac ggctggcagc cgggcggcgc ggtgatgttc ccggcgcggg tcgagggcga
     8341 agcgtggcgc gcctacgccg gcatcgagcc gaagcaccgc ctcgccgacc tgaagcgcgc
     8401 cggcgcccgg ttcgacgcgc cggccgagct cgacggcgcg ctcggcgcgc tggtcgagct
     8461 ggcgacgccc gagcgcgcga gcgagtaccg cgtcgggctc aacaacttct acgtgctgac
     8521 gcgctacaac cgcagcgcgc tgtacgccac ggcggtcagc gagctggcag cggcactcag
     8581 gaaggctcag gaggccggca agtagcgcat cgggtcgacc gccgccgagc ctttgcggat
     8641 ctggaagtgc agcttcggcc ggtcggcgtc ggtgttgccg agctcggcga tcttctgccc
     8701 gcgcgtcacg ctctgctgct ccttcaccag gatgtcccgg ttgtgcgcgt agacggtgat
     8761 gaagccgttg tcgtgcttga tcaccaccag cttgcccatg ccggggatgc cggtgccgat
     8821 gtaggtgacg cgcccggcgg cggcggcgag caccgggtcg ccgagcttgc cgtcgatgtc
     8881 gatgcccttg ctgcgcggct cggcgaaccc cgccagcacc ttgcccttga ccggccagat
     8941 gaaatcgacg ccctcgccct cggccttggg ctcctcgcgc gcggccatgg cggcgttttc
     9001 cttcgagtac ggcaggcgca gcgccttcgg ctcggtcttc gccgccccct cggccgacgc
     9061 cggcggcgcc gagtcgatcg agcgcgattc gacgcgcccg gcgccgcgcg cgctgcccac
     9121 caccacgccg gggcgcgcgt cgggcgcacg aggttccggc ttgacgcgca gcacctggcc
     9181 gatacgcagc ttggtcgggt cgtcgagcgc gttccactgc gccacttcgc ggtagtcggc
     9241 gccgttgtcg agcgcgatgc tgtagagcgt gtcgccctgc ttgacggtat agaagccgtc
     9301 ggtcgcgggc gccgccgcag gcttcgccgc ctgtgcggct gcggccgcgg gcttgctcgc
     9361 cgctttggag gcgcgcgcgc gctcctcgac cggggccggc ccgcgatagg tcgagcaggc
     9421 cgccgcgccg agcgcgagcg ccatcaccgc caccgccgcc agcctcaccc tgttcatgcc
     9481 tttcccgcct ccatcggcac gaaacgcacc gtgtcgagct cggtttcgac gaagccgcgg
     9541 ccgctgcgct cgacgagcac caggcgctgc gcgccgttgg caccatcgcg caacggcagc
     9601 accattctgc caccgggcgc gagctggcgt aacagcgcct cgggcacgct gcgcgccgcg
     9661 gctgcgacca cgatggcctg gaacggcgcc gccttctcca gcccggcata gccgtcgccg
     9721 tgcaccaggc gcaggttgga cagcttgagg ccgaggagat tcgcgcgcgc gcgctcgtgc
     9781 agacccttga tgcgctcgat cgaatagacc tcgctgaaga tctgcgccag caccgccgcc
     9841 tggtagccgc agccggtgcc cacctccagc accttgccgg gggatttttc atttgcgatc
     9901 aagcattcga tcatgcgtgc gacgatgtag ggctgcgaga tgg
//
LOCUS       DASYJA010000292         4921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_314890, whole genome shotgun sequence.
ACCESSION   DASYJA010000292 DASYJA010000000
VERSION     DASYJA010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314890"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /locus_tag="VGO02_06335"
     CDS             complement(<1..1186)
                     /locus_tag="VGO02_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEV7477338.1"
                     /translation="MPSVAEQFKIHASEYYAPKNFNFWYYFGSLALLVLVIQIVSGIF
                     LTMNYKPDAALAFGSVEYIMRDVPAGWLLRYIHTTGASAFFIVVYLHMFRGLLYGSYK
                     QPRELVWIFGVLIYLTLMGEAFFGYLLPWGQMSYWGAQVIVNLFGTVPFVGPDLALWI
                     RGDYVVSDATLNRFFALHVIAFPLVLLGLIVAHILALHDVGSNNPDGVDIKKVKGPDG
                     HPLDGVPFHPYYTVKDIMGAVVFLFIFCFVMFFVPEVGGYFLEYNNFIPADPLKTPPH
                     IAPVWYFTPYYSILRAVPPLFNSQFPGVLAMGIATLILFFLPWLDRSPVKSIRYRGVL
                     YKSWLAAFVVTFIVLGYLGTENTNVWGQFGFDAFFLDTKDRATWMARLCTILYFAFFF
                     LMP"
     gene            complement(1235..1831)
                     /gene="petA"
                     /locus_tag="VGO02_06340"
     CDS             complement(1235..1831)
                     /gene="petA"
                     /locus_tag="VGO02_06340"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEV7477339.1"
                     /translation="MSDQEKVDKTRRNLVVATSAVGAAAGVGVALPFAASMLPSEKAK
                     AAGAPVEFDISRVAPGELAVIEWRGKPVWVIHRTKEMLESLKAATPRLVDPDSKSSEQ
                     PKYAKNENRSENPEWMVMEGVCTHLGCSPQLKPAEAKAEMGADWVGGFYCPCHGSKFD
                     LAGRVFRGAPAPTNLKVPPYTLTDTKLVIGEDKETKGA"
     gene            1956..3125
                     /locus_tag="VGO02_06345"
     CDS             1956..3125
                     /locus_tag="VGO02_06345"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEV7477340.1"
                     /translation="MYRLWIIFAQAATVAVAVVFVVSTFRPEWLPVRSTPGGTGTLVQ
                     QAPTAGPLPGAASVGSYSEAVRRATPSVVNIFSSKEIRAPRHPLLNDPIFRRFFGDQL
                     PDEAQRASSLGSGVIVSSSGYLLTNHHVVEAADEIEVALPDGKKLLAKVVGNDPETDL
                     AVLRVDAENLPAITFGSSDALRVGDVVLAIGNPFGVGQTVTGGIVSALGRTGLGINTF
                     ENFIQTDAAINPGNSGGALIDAGGNLVGINTAIFSRSGGSMGIGFAIPVSTAKMVLEQ
                     IVKTGSVTRGWIGVEVQELTPQAAESFKLGETRGALIAGVLRGGPADKAGVKPGDVLY
                     EIEGKPVVDPSGMLNVVAALAPGKSAHMKLKRKGQDVNADVTVGTRPKPQPRVPD"
     gene            3135..>4921
                     /locus_tag="VGO02_06350"
     CDS             3135..>4921
                     /locus_tag="VGO02_06350"
                     /inference="COORDINATES: protein motif:HMM:NF022073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0047989 - hydroxybutyrate-dimer hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate oligomer hydrolase family
                     protein"
                     /protein_id="HEV7477341.1"
                     /translation="MRFGFSLVVLFAAGCAGTPDFSGQKILHTRYDGNTDDLLTAGLG
                     RTGLGLATPPAVREAKSPSVAELRRRAIHANYRALADMSPAGGYGVLYGPNIDLAGKD
                     TLGEGKVAGDEYLTLFDGSTLMVQVPERFDPKNPCIVTASSSGSRGIYGAIGTVGEWG
                     LKRGCAVAYTDKGTGTGFHDLATDSVNVVSGEWMIREKAGSDALFAAALSDAERTRYL
                     REYPNRIAVKQAHSQRNPEKDWGRYTLAAVDFSFYIINSLKDARAGTYTPENTIVIAS
                     SVSNGGYAALAAAEQDKRGLIDGVAVSEPNVSLAGVRPLLDYMTLLNLYEPCAALAVA
                     NAPLNTVSQPLKEARCASLAKKGLLKAPDLAGQAGESQKILLDYGFLPEAALTLPSHY
                     TLYVPQSISLTYANSYGRFAVTDNLCGYSFAATAPSGDNAAQPIPAAGLELSFASSNG
                     IPPTGGVNLVNNLSTGGAREDRLSISPSSGARDMNLDGALCLRSLATGLDAAGQPLTA
                     TLLEQHRRVQAGIVETQLTGNLQGKPAVFVNGRADAILPPNFASRAYFARNKTVEPGS
                     KLYYYEVTNAHHLDVLNGVAGFDSRYLPL"
BASE COUNT          869 a         1686 c         1614 g          752 t
ORIGIN      
        1 agggcatcag gaagaagaac gcgaagtaga ggatcgtgca caggcgcgcc atccaggtgg
       61 cgcggtcttt cgtgtcgagg aagaaggcgt cgaagccgaa ctgcccccag acgttggtgt
      121 tctcggtgcc gagatagccg aggacgatga acgtcaccac gaaggcagcc agccacgact
      181 tgtacagcac gccgcgatag cggatcgact tcaccgggct gcggtcgagc cagggcagga
      241 agaacaggat cagggtcgcg atgcccatgg cgagcacgcc cgggaattgc gaattgaaga
      301 gcggcggcac ggcgcgcagg atcgagtagt agggcgtgaa gtaccagacc ggcgcgatgt
      361 gcggcggcgt cttcagcgga tcggccggga tgaagttgtt gtactcgagg aagtagccgc
      421 cgacctcggg cacgaagaac atcacgaagc agaagatgaa gagaaacacc acggcgccca
      481 tgatgtcctt caccgtgtag tagggatgga acggcacgcc gtcgagcggg tggccgtcgg
      541 ggcccttcac cttcttgatg tcgacgccgt cggggttgtt cgagccgacg tcgtgcagcg
      601 cgaggatgtg cgccacgatc agcccgagca gcaccagcgg gaaggcgatg acgtgcagcg
      661 cgaagaagcg gttgagcgtc gcgtctgaca cgacatagtc gccgcggatc cacagcgcga
      721 ggtcggggcc gacgaacggc accgtaccga acagattgac gatcacctgc gcgccccagt
      781 aggacatctg gccccagggc agcaggtagc cgaagaacgc ctcgcccatc agcgtgaggt
      841 agatcagcac gccgaagatc cagaccagct cgcgcggctg cttgtacgag ccgtacagca
      901 ggccgcggaa catgtgcagg tagacgacga tgaagaacgc cgaggcgccg gtggtgtgga
      961 tgtagcgcag cagccagccc gccggcacgt cgcgcatgat gtactcgacc gagccgaagg
     1021 cgagcgcggc gtcgggcttg tagttcatcg tcaggaagat gcccgagacg atctggatca
     1081 ccagcaccag cagcgcgagc gagccgaagt agtaccagaa gttgaagttc ttcggcgcgt
     1141 agtactccga agcgtggatc ttgaactgct cggcgaccga gggcatcctc gcgtcgaacc
     1201 agccccacag gcgcgatacc agcgtcccca tcgctcaggc tcccttggtt tctttgtctt
     1261 cgccgatgac cagtttcgtg tcggtcagcg tgtagggcgg caccttgagg ttcgtcggcg
     1321 ccggcgcgcc gcggaacacg cggccggcga gatcgaactt cgagccgtgg cacgggcagt
     1381 agaagccgcc gacccagtcg gcgcccattt ccgccttcgc ctcggcgggc ttgagctgcg
     1441 gcgagcagcc gaggtgggtg cacaccccct ccatcaccat ccactcgggg ttctccgagc
     1501 ggttctcgtt cttcgcgtac ttgggctgct cggaggactt cgagtccgga tcgaccagcc
     1561 gcggcgtggc cgccttgagc gactcgagca tttccttggt gcggtggatc acccacaccg
     1621 gcttgccgcg ccactcgatc accgccagct cgcccggcgc gacgcggctg atgtcgaatt
     1681 ccaccggcgc gccggccgcc ttggccttct cggagggcag catgctggcg gcgaacggga
     1741 gcgcgacgcc gacaccggct gcggcgccca cggccgacgt ggcaaccacc aggttgcggc
     1801 gcgtcttatc tactttttct tgatcgctca tggagatggg ggggttttag ccgtgcgcaa
     1861 aagtgtgcga ttctagcgac aggccccgga aaagtctacg atttcgcgcc taaacgctta
     1921 aatttgcaga agaatttcgt atactagaat tcttcatgta tcgcctctgg atcatcttcg
     1981 cgcaggcggc cacggtcgcc gtggcggtgg tcttcgtggt ctcgacgttc cggcccgaat
     2041 ggctgccggt gcgcagcacg ccgggcggga cgggcacgct cgtgcagcag gcgcccaccg
     2101 cagggccgct gcccggcgcg gccagcgtcg gctcctacag cgaagcggtg cgccgcgcca
     2161 cgccctcggt ggtgaacatc ttcagctcga aggaaatccg cgcgccgcgc cacccgctgc
     2221 tcaacgaccc tatcttccgc cgcttcttcg gcgaccagct gcccgacgag gcgcagcgcg
     2281 cctccagcct gggctccggc gtgatcgtca gctcgagcgg ctacctgctc accaaccacc
     2341 atgtcgtcga ggccgccgac gagatcgagg tggcgctgcc cgacggcaag aagctgctcg
     2401 ccaaggtggt cggcaacgac cccgagaccg acctcgcggt gctgcgcgtt gacgccgaga
     2461 acctgccggc gatcaccttc ggctcctccg acgcgctgcg cgtcggcgac gtggtgctcg
     2521 ccatcggcaa tcccttcggc gtcggccaga cggtcaccgg cggcatcgtc tcggcgctcg
     2581 gccgcacggg cctgggcatc aataccttcg agaacttcat ccagaccgac gccgcgatca
     2641 acccgggcaa ctcgggcggc gcgctgatcg acgccggcgg caacctggtc ggcatcaaca
     2701 ccgcgatctt ctcgcgctcc ggcggctcga tgggtatcgg cttcgccatc ccggtgtcga
     2761 ccgcgaagat ggtgctcgag cagatcgtca agaccggcag cgtgacgcgt ggctggatcg
     2821 gcgtcgaggt gcaggagctc acgccgcagg ccgccgagtc gttcaagctc ggcgagacgc
     2881 gcggcgcgct gatcgccggg gtgctgcgcg gcggcccggc cgacaaggcg ggggtcaagc
     2941 ccggcgacgt gctctacgag atcgagggca agccggtggt cgatccctcg ggcatgctca
     3001 acgtcgtcgc cgcgctcgcc ccgggcaaaa gcgcgcacat gaagctgaaa agaaaaggac
     3061 aggacgtcaa cgccgatgtt accgtcggca cgcggcccaa gccgcagccg cgcgttccag
     3121 actaggggga gaaaatgaga ttcggttttt cgctggttgt gctgttcgcc gccggctgcg
     3181 ccggtacgcc cgatttttcc ggccagaaga tccttcacac gcgctacgac ggcaacaccg
     3241 acgacctgct caccgccggc ctgggccgga ccggcctcgg cctggcgacg ccgcccgcgg
     3301 tgcgcgaggc gaagagcccg agcgtcgcgg agctgcgccg cagggcgatc cacgccaact
     3361 accgcgcgct cgccgacatg agcccggcgg gtggctacgg cgtgctgtac gggccgaaca
     3421 tcgacctggc gggcaaagac acgctgggtg aaggcaaggt cgccggcgac gaatacctca
     3481 cgctgttcga cggctcgacc ttgatggtcc aggtgcccga gcgcttcgac ccgaagaacc
     3541 cctgcatcgt caccgccagc tcctcgggct cgcgtggcat ctacggcgcc atcggcaccg
     3601 tgggcgaatg ggggctgaaa cgcggctgcg ccgtggccta caccgacaag ggcaccggca
     3661 ccggcttcca cgacctcgcc acggactccg tgaacgtcgt cagcggcgag tggatgatcc
     3721 gcgagaaggc gggcagcgac gccctgttcg cggcggcgct gtccgatgcc gagcgcacgc
     3781 gctacctgcg cgaatatccg aaccgcatcg cggtgaagca ggcccactcg cagcgcaacc
     3841 ccgagaagga ctggggccgc tacacgctgg ccgcagttga tttttctttt tacatcatta
     3901 attcgctaaa ggatgcgcgc gccgggacgt atacgccgga gaacaccatc gtcatcgcct
     3961 ccagcgtctc gaacggcggc tacgccgcgc tcgccgcggc cgagcaggac aagcgcggcc
     4021 tgatcgacgg cgtcgccgtc tccgagccga acgtatcgct cgccggggtg cgcccgctcc
     4081 tcgactacat gacgctgctc aacctctacg agccctgcgc cgcgctcgcc gttgcgaacg
     4141 cgccgctcaa caccgtgtcg cagccgctga aagaggcgcg ctgcgcatcg ctcgcgaaga
     4201 aaggcctgct caaggcgccc gacctcgccg gccaggccgg cgagagccag aagatcctgc
     4261 tcgactacgg cttcctgccg gaggcggccc tcacgctgcc ctcgcactac acgctgtacg
     4321 tgccgcagag catctcgctg acctatgcca acagctacgg ccgcttcgcg gtgaccgaca
     4381 atctgtgcgg ctacagcttc gccgccacgg cgccttccgg cgacaacgcc gcgcagccga
     4441 tcccggcggc gggcctcgag ctgtccttcg ccagctcgaa cggcatcccg ccgaccggcg
     4501 gcgtcaacct ggtgaacaat ctctccaccg gcggcgcgcg cgaggaccgg ctgtcgatct
     4561 cgccttccag cggcgcccgc gacatgaacc tcgacggcgc gctgtgcctg cgctcgctcg
     4621 ccaccggcct cgacgccgcc ggccagccgc tcaccgcaac gctgctcgag cagcaccgcc
     4681 gcgtgcaggc cggcatcgtg gagacccagc tcacgggcaa cctgcagggc aagcccgcgg
     4741 tcttcgtcaa cggccgcgcc gacgccatcc tgccgccgaa cttcgcctcg cgcgcgtact
     4801 tcgcgcgcaa caagacggtc gagcccggct cgaagctcta ctactatgaa gtcaccaacg
     4861 cccatcacct cgacgtgctc aacggtgttg cgggcttcga ctcccgctac ctgccgctgc
     4921 a
//
LOCUS       DASYJA010000293         4885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1223242, whole genome shotgun sequence.
ACCESSION   DASYJA010000293 DASYJA010000000
VERSION     DASYJA010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223242"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..133
                     /locus_tag="VGO02_06355"
     CDS             <1..133
                     /locus_tag="VGO02_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEV7477342.1"
                     /translation="GILSGAHAAEKRAAGASLVQLYTGLIYRGPALVPECVSAFRAK"
     gene            106..1146
                     /locus_tag="VGO02_06360"
     CDS             106..1146
                     /locus_tag="VGO02_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEV7477343.1"
                     /translation="MRIGVPREIKDGEFRVALTPQGVKGLSNVVIEPGAGKGVGFSDQ
                     DYVKAGASLGDPWECDLVVKVKELQEAEYRRPRRGQTVFGFQHFGPAPQLLDAALASG
                     ATFIAYETVGEAAGLPILSPMSAIAGRLAVLAGAWCLQKQNGGSGVLLSGVAGVAPGR
                     IAILGAGNVGSNALAVADGIGAEVIVIARSETRFAALQRRFPRAEFRTEGLEQAVRHA
                     DLVIGGVLTPGRMSPKLVTRAMLARMRPGSALVDVGIDQGGISETSRPTSYSAPTYVE
                     EGVVHYCVPNMPAACARTASLALEGAVLPFVLQLAKESCDENLKTGIQVRAGQVTHQH
                     LARDTKRAYTAL"
     gene            1158..1403
                     /locus_tag="VGO02_06365"
                     /pseudo
     CDS             1158..1403
                     /locus_tag="VGO02_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821324.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
     gene            1478..2116
                     /gene="nth"
                     /locus_tag="VGO02_06370"
     CDS             1478..2116
                     /gene="nth"
                     /locus_tag="VGO02_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV7477344.1"
                     /translation="MNPAKRAEIFRRLQAANPHPTTELKYRTPYELLVAVVLSAQATD
                     KSVNLATVELFKVADAPHKMVKLGVAGLEQYIKRIGLYRNKAKNVVALSEILIREHGG
                     QVPETREALESLPGVGRKTANVVLNTAFGQPTMAVDTHIFRVANRTGLAPGKDVLSVE
                     EKLLKFTPLEYLMNAHHWLILHGRYVCLARKPDCPRCAIRDLCEFKGKTKDV"
     gene            2109..2531
                     /locus_tag="VGO02_06375"
     CDS             2109..2531
                     /locus_tag="VGO02_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEV7477345.1"
                     /translation="MFDPTREQVRDMFFAAWQKYRAGQPLAGIESLALDVILLHPEYH
                     GVLSLPTKFREKDYTDQSNPFLHMSLHVAFEEQLSIDQPPGIVARWRELLAHHGDRHE
                     ALHQAIECLAETMWRAQRDKAPPDAAAYLECLGRRGRA"
     gene            2625..3209
                     /locus_tag="VGO02_06380"
     CDS             2625..3209
                     /locus_tag="VGO02_06380"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEV7477346.1"
                     /translation="MNPLLLRLGLLLALLWPVAALPADPSESVILVAKRELQDRIYGA
                     SVLLVRPLDGERHVGVIINKPTQTQLGTLFPDHAPSRKVVDPVFLGGPIGSEVIFALV
                     QRKDSPGNRSLRIGPDLYLATDSRVVDQIIESQPSQARFFAGVVLWNQGELAEELRRG
                     LWFVQESRSEIVLRKPAGLWEELVNRLEKAAKAI"
     gene            complement(3210..3536)
                     /locus_tag="VGO02_06385"
     CDS             complement(3210..3536)
                     /locus_tag="VGO02_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEV7477347.1"
                     /translation="MRMIFAAVALAVSFSVQAGNTGKGKELVTAKACATCHGEQGNKP
                     SAPDQPVLAGQYNDYLVRALTDYKSGRRNNPIMKGFAGQLSKQDIEDLAAWFSSQKAT
                     ALHDQR"
     gene            complement(3533..3916)
                     /locus_tag="VGO02_06390"
     CDS             complement(3533..3916)
                     /locus_tag="VGO02_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEV7477348.1"
                     /translation="MPRILISAVLGAMLAFGAQLALAQAKKAAKKAAEPAAQKEIVGD
                     PDNAHRNKTAMCIGCHGIPGYKTAFPDVYHVPKIAGQQPAYLVNALKAYKSGERSHPS
                     MRGIAASLTDQDMADLAAYYGVPAK"
     gene            4002..4844
                     /locus_tag="VGO02_06395"
     CDS             4002..4844
                     /locus_tag="VGO02_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008350690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEV7477349.1"
                     /translation="MRFKGSPSYVSTEDLTLAVNAAITLQRPLLVKGEPGTGKTMLAV
                     EVANALGVPLLEWHIKSTTKAQQGLYEYDAVSRLRDSQLGDERVHDIHNYIVKGMLWN
                     AFTQDKPCVLLIDEVDKADIEFPNDLLRELDRMEFYVYETRELVKAKHRPIVFITSNN
                     EKELPDAFLRRCFFHYIRFPDKETMEKIVGVHFPHLKKSLLREAMEVFFEVREVPGLK
                     KKPSTSELLDWLKLLLAEDISPEALRSKDRKTIIPPLHGALLKNEQDVHLFEQLIFLQ
                     KRGR"
BASE COUNT          854 a         1632 c         1603 g          796 t
ORIGIN      
        1 cggcatcctg tccggcgcgc acgccgccga aaaacgcgcg gccggagctt cgctcgtgca
       61 gctctacacc ggcctgattt accgcggccc ggcgctggtg cccgaatgcg tatcggcgtt
      121 ccgcgcgaaa taaaggacgg cgagttccgg gtcgctttga cgccccaggg ggtgaagggc
      181 ctttcgaacg tcgtgatcga gcccggcgca ggcaaaggcg tcgggttttc cgaccaggac
      241 tatgtgaagg ccggcgccag cctcggcgat ccgtgggagt gcgacctggt tgtcaaagtg
      301 aaggagctgc aggaggccga ataccgccgc ccgcggcgcg gccagaccgt gttcggcttc
      361 cagcacttcg gccccgcgcc gcagctgctc gacgcggcgc tcgcctcggg cgccactttc
      421 atcgcctacg aaaccgtcgg cgaggccgcc ggcctgccga tcctctcgcc catgtcggcg
      481 atcgccgggc gcctggcggt gctggcgggc gcctggtgcc tgcagaagca gaacggcggc
      541 agcggcgtgc tcctgtccgg cgtcgccggg gtggcgcccg gcaggatcgc gatcctcggc
      601 gccggcaacg tcggctcgaa cgctctcgcc gtcgcggatg gcatcggcgc cgaggtcatc
      661 gtgattgcgc gaagcgaaac gagatttgcg gctttgcaac gccgcttccc gcgcgcggag
      721 tttcgaaccg aaggacttga acaggcggtc cgccatgcgg acctggtcat agggggcgtg
      781 ctgacgcccg ggcggatgtc gccgaagctc gtcacccgcg ccatgctcgc gcgcatgcgt
      841 ccgggctcgg cgctggtcga cgtcggcatc gaccagggcg gcatcagcga aacctcgcgg
      901 ccgacctcct acagcgcgcc gacctacgtc gaggaaggcg tggtgcacta ttgcgtgccc
      961 aacatgccgg ccgcgtgcgc gcgcaccgcg agcctggcgc tggagggagc ggtgctgccc
     1021 tttgtcctgc agttggcaaa ggaatcctgt gatgaaaatc tcaagaccgg aatacaagtc
     1081 cgcgccggcc aggtgacgca ccagcacctc gcgcgcgata cgaagcgcgc ctacactgcc
     1141 ttatgaaacg cgaggccgtc gcgctgatcg acgaggcgcg ctgcatcggc tgcacgctgt
     1201 gcatcgaggc ctgcccggtc gacgccatcg tcggcgcgca aagcctgatg cataccgtgg
     1261 tggagccctg gtgcatcggc tgcgcgctct gcctgccgcc ctgcccggtc gactgcatcg
     1321 acatggtcgc ggcgcaaggc gcctggaccg ccggcctgaa aaacgcggcc aagcagcggg
     1381 tacggagccg gaaaagccga attgccggga tgaaagcaat ttccggggtg aaagatcgaa
     1441 aggccgtgat cggcgccgtc ctggcgcgcc gcaagcgatg aatcccgcca agcgcgccga
     1501 gatcttccgc cgcctgcagg cggccaaccc gcaccccacg accgagctca agtaccgcac
     1561 gccttacgag ctgctggttg cggtcgtgct ctccgcccag gcaacggaca aatcggtaaa
     1621 tctcgcgacg gtcgagctct tcaaggtggc tgatgcgccg cacaagatgg tgaagcttgg
     1681 cgtcgccggc ctggagcagt acatcaagcg catcgggctc taccggaaca aagcgaagaa
     1741 cgtcgtggct ctttcagaaa tactcatccg cgagcacggc ggacaggtac ctgaaacaag
     1801 agaggcgctc gagagcctgc ccggggtagg ccgcaagacc gccaacgtgg tgctcaacac
     1861 cgccttcggc cagccgacga tggcggtgga cacgcacatc ttccgcgtcg ccaaccggac
     1921 cggcctcgca ccaggaaagg atgttttatc tgtcgaagaa aaacttttga aattcacgcc
     1981 gctcgaatac ctgatgaacg cgcaccactg gctcatcctg cacggccgct acgtctgcct
     2041 ggcgcgcaag ccggattgcc cgcgctgcgc gatccgcgac ctgtgcgagt tcaagggcaa
     2101 gacaaaggat gtttgacccg acccgcgagc aggtgcgcga tatgttcttc gccgcctggc
     2161 agaagtaccg cgccgggcag ccgctcgccg gcatcgagtc gctggccctt gacgttatcc
     2221 tgctacaccc ggaataccac ggcgtgctca gcctgccgac caagttccgc gagaaggact
     2281 acaccgacca gagcaatccg ttcctgcaca tgagcttgca cgtcgcgttc gaggagcagc
     2341 tctcgatcga ccagccgccg ggcatcgtgg cgcgctggcg ggaactgctc gcgcaccacg
     2401 gcgaccggca cgaagccttg caccaggcga tcgagtgcct ggcggagacg atgtggcggg
     2461 cgcagcgcga caaggcgccg cccgacgcgg ccgcctacct cgagtgcctc gggcgccgcg
     2521 ggcgcgcttg agctgccggt tcgcggcgac agggccgcag cggacaccaa ctggactagc
     2581 cgcagcgccg cgttgtgaat tgtttcgtcc cgcatgatgg cgccatgaac cccctcctgc
     2641 tccgcctcgg cctgctgctc gcgctgctct ggcccgtcgc cgccctgccg gccgacccct
     2701 ccgaaagcgt gatcctcgtg gcgaagcggg agctgcagga ccgcatctac ggcgccagcg
     2761 tgctgctcgt gcggccgctg gacggcgagc gccacgtcgg ggtgatcatc aacaagccga
     2821 cccagaccca gctcggcacg ctgttcccgg accacgcccc gtcgcgcaag gtggtcgacc
     2881 cggtgttcct cggcggcccg atcggctcgg aagtcatctt cgcgctggtc cagcgcaagg
     2941 acagcccggg caaccgctcg ctgcgcatcg gcccggatct ctacctcgcc accgacagcc
     3001 gggtggtcga ccagatcatc gagtcgcagc cgtcgcaggc gcgcttcttc gccggggtgg
     3061 tgctgtggaa ccagggcgag ctcgccgagg agctcaggcg cggcctctgg ttcgtgcagg
     3121 agtcgcgcag cgaaatcgtg ctgcgcaagc cggccggtct gtgggaagag ctggtcaacc
     3181 gcctggagaa ggcggccaag gcgatctagc tagcgctggt cgtgcagggc agtggccttc
     3241 tggctggaga accaggcggc gagatcctcg atatcctgct ttgaaagctg gccggcgaag
     3301 cccttcatga tcgggttgtt tcggcgcccg ctcttatagt ccgtaagcgc cctcaccaga
     3361 tagtcgttgt actggccggc aagcaccggc tgatcgggcg ccgagggctt gttcccctgc
     3421 tcgccgtggc acgtggcgca ggccttcgcg gtgaccagct ccttgccctt gccggtgttg
     3481 cctgcctgga cggaaaacga gaccgcaagc gcgacggcgg cgaaaatcat cctcatttgg
     3541 cgggcacccc gtaataggcg gcgaggtcgg ccatgtcctg gtcggtcagg ctggcggcga
     3601 tgccgcgcat cgacggatgc gagcgctcgc cggacttgta ggccttgagc gcgttgacca
     3661 ggtacgccgg ctgctggccg gcgatcttcg gcacgtggta cacgtcggga aacgcggtct
     3721 tgtagccggg gatgccgtgg cagccgatgc acatcgcggt cttgttgcga tgcgcgttgt
     3781 cggggtcgcc gacgatctct ttctgcgctg ccggctctgc cgctttcttg gccgccttct
     3841 tcgcctgcgc gagcgcgagc tgcgcgccga aagcgagcat cgcgccgagg accgcactaa
     3901 tcaggattcg aggcatccgc cgttcttgta agtatgtgag accgcgggaa ttctagcgaa
     3961 agccgctgct agaatccaat tttgatcgcc gaaagcgtga aatgaggttc aagggctcgc
     4021 cctcctacgt ctccaccgag gacctgacgc tcgcggtcaa cgccgccatc acgctgcagc
     4081 ggccgctgct cgtcaagggc gagccgggga ccggcaagac gatgctcgcg gtcgaggtgg
     4141 cgaacgcgct cggcgtcccg ctgctcgagt ggcacatcaa gtccaccacc aaggcgcagc
     4201 agggcctgta cgagtacgac gcggtcagcc gcctgcgcga ctcgcaactc ggcgacgagc
     4261 gggtgcacga catccacaac tacatcgtca agggcatgtt gtggaacgcg ttcacgcagg
     4321 acaagccctg cgtgctgctg atcgacgagg tcgacaaggc cgacatcgag tttcccaacg
     4381 atctcctgcg cgagctcgac cggatggagt tctacgtcta cgagacgcgc gagctggtca
     4441 aggcaaagca tcgcccgatc gtcttcatca cctcgaataa cgagaaggag ctgccggatg
     4501 cattcctgcg ccgctgcttc ttccactaca tccgcttccc cgacaaggag acgatggaga
     4561 agatcgtcgg cgtgcacttc ccgcacctca agaagtcgct gctgcgcgag gcgatggaag
     4621 tgttcttcga agtgcgcgag gtgcccggcc tgaagaaaaa gccctcgacc tccgagctcc
     4681 tcgactggct gaagctcctg ctcgccgagg acatctcgcc cgaagccctg cgctccaagg
     4741 accgcaagac catcatcccg ccgctgcacg gggcgctcct caagaacgag caggacgtgc
     4801 atttgttcga gcagctcatc ttcctgcaga agcgcggccg gtagcggtcc tttccagcca
     4861 aggaggcagc catggcgaca aagcg
//
LOCUS       DASYJA010000294         4055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1586586, whole genome shotgun sequence.
ACCESSION   DASYJA010000294 DASYJA010000000
VERSION     DASYJA010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586586"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="VGO02_06400"
     CDS             complement(<1..175)
                     /locus_tag="VGO02_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NodJ"
                     /protein_id="HEV7477350.1"
                     /translation="MARRNYYALPSLSLRWIAVWRRNVLVWQKLAIPSILGNLADPLI
                     MLFGLGYGLGAMVG"
     gene            complement(175..1086)
                     /locus_tag="VGO02_06405"
     CDS             complement(175..1086)
                     /locus_tag="VGO02_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7477351.1"
                     /translation="MRSVLSVRGLRKSFGDLEVVCGVDLEVAPGECFGLLGPNGAGKT
                     TTLKLCLGLADPDAGDIELLGEEVPKRARQARARVGVVPQFDSLDPDFTVAENLLVFG
                     RYFGISQREMAGRIPALLEFAGLAARSAARINTLSGGMKRRLTLARALVNDPQLIVMD
                     EPTTGLDPQASHLIWERLRPLTQQGKTLVLTTHFMEEAERLCQRLAIMDHGRIIAQGS
                     PRALIAEHAEAQVVEVHGPGLDSWAERAKRLAPRVERVGDTVFCYAENVDSILQDLSL
                     KSDLTYLHRAASLEDVFLRLTGRDLRD"
     gene            complement(1106..1696)
                     /locus_tag="VGO02_06410"
     CDS             complement(1106..1696)
                     /locus_tag="VGO02_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7477352.1"
                     /translation="MRASTRREFVAGPAGRIECAVDAPDSPARGAALVAHPHPLFGGT
                     LDNKVAQTLARTFVDLGYEAWRPNFRGVGQSEGKHDAGRGEVDDLEAVYKSIDRQPMV
                     LAGFSFGAAMQARLAQRVSSERLVLVGVAVVDREVPPVPAGTLVIHGELDTTIPLAAV
                     LEWARPQDLTVVVVPGADHFFHRKLQELRDLVKANW"
     gene            complement(1693..2004)
                     /locus_tag="VGO02_06415"
     CDS             complement(1693..2004)
                     /locus_tag="VGO02_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEV7477353.1"
                     /translation="MSYYEKHVFFCCNQRDGGDRPCCNAKGASEMRDYAKKRVKEKGL
                     AGEGKVRINQAGCLDRCEEGPCVVVYPDAVWYTYVDRADIDEIIEEHLQHGRVVERLR
                     M"
     gene            complement(2014..3009)
                     /locus_tag="VGO02_06420"
     CDS             complement(2014..3009)
                     /locus_tag="VGO02_06420"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV7477354.1"
                     /translation="MILIAYASLYPLEGWRDPGTSPFAFLLQDWPRQLLRFDMAVNVL
                     AYAPFGFLCVAALQPRLRGGAAAAAATAAALVLSLSLEALQSYLPARFASNLDMACNV
                     AGAALGAAAAPALLARLDGLRLRERLFLPGPDIDAGLVLLALWLFVQLNPATLLFGAG
                     DLRPLIAPVAARAHAAEFFIRTEAFIAAFNLVAVGLLASLLVAPGQRLRAMFVAFVVA
                     ALAVKAIAFALMMQSEAFAWFTPGAQAGLAAGLAVALAAVALPRTVRLVLAAVLVMAA
                     TVFVNLAPPNPYLAATLKVWQQGTFLNFNGLTRLVGALWPFAALGYLIFLAARRR"
     gene            complement(3078..>4055)
                     /locus_tag="VGO02_06425"
     CDS             complement(3078..>4055)
                     /locus_tag="VGO02_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEV7477355.1"
                     /translation="ISGVVVLAYTIWGGMWSVAMTDLFQSVMIILGLGIIAWFVGDMA
                     GGPGKVIAAAAEGGKFEFWPKGGAKEWLAFVTAFLTLAIGSIPQQDIFQRVTSAKNEK
                     TAILGTLAGGVIYFSFAFVPIFIVYAALIIDPSLGRLLSAEDAREMQSILPAFILGHT
                     PMWIQILFFGALLSAILSTASGAIIAPTSLITENVVRQFFPRMTDRQFLATLRAILVG
                     FTVAALVFALLSKKTMYDMIQNAYTVTLVAAFVPLAAGIFWKRATNTGAIASSLGGLA
                     AWSAVEALATDPTLPSPLVGLVFSAIGMVAGSLVPRGQTQAQSAHGGHG"
BASE COUNT          711 a         1365 c         1394 g          585 t
ORIGIN      
        1 cgccgaccat cgcgcccagg ccgtagccca ggccgaacag catgatcagc gggtcggcca
       61 ggttgccgag gatcgatgga atggcgagct tctgccacac cagcacgttg cggcgccaca
      121 ccgcgatcca gcgcaggctg agcgagggga gagcgtagta gttgcgccgc gccatcaatc
      181 cctcaggtcg cggccggtga gcctgaggaa cacgtcttcg aggctcgccg cgcgatgcag
      241 atacgtcaga tcggatttta aagaaagatc ttgcaaaatc gaatccacgt tttcggcgta
      301 gcagaagacc gtgtcgccca cccgctcgac gcgcggcgcg aggcgcttcg cccgctccgc
      361 ccagctatcc aggccggggc cgtgcacctc gacgacctgc gcctcggcgt gctcggcgat
      421 gagcgcgcgc ggcgagccct gcgcgatgat gcggccgtgg tccatgatgg cgaggcgctg
      481 gcacagacgc tcggcctcct ccatgaagtg cgtggtcagc accagcgtct tgccctgctg
      541 cgtgagcggg cgcagccgct cccagatcag gtggctcgcc tgcggatcga gcccggtggt
      601 cggctcgtcc atgacgatga gctgcgggtc gttcaccagg gcgcgggcga gcgtgagccg
      661 gcgcttcatg ccgccggaaa gtgtgttgat gcgggccgcg ctgcgcgccg ccaggccggc
      721 gaactccagc agcgccggga ttcgcccggc catctcccgc tgcgaaatgc cgaaatagcg
      781 cccgaacacg agcaggttct cggccaccgt gaaatccggg tcgaggctgt cgaactgcgg
      841 caccacccca acgcgggctc gcgcttggcg ggcccgcttt gggacctcct cacctaacaa
      901 ttcgatgtcg cccgcgtcgg gatcggcgag gcccaggcag agcttcaggg tggtagtctt
      961 gcccgcgccg ttggggccga gcaggccgaa gcactcgccg ggcgccacct cgaggtcgac
     1021 gccgcatacc acctcgagat cgccaaacga cttgcgcagc ccgcgcacgg acagcacgct
     1081 cctcattttc ccctcgtttt cccgatcacc agtttgcttt gaccaggtcg cgcagctctt
     1141 gcagcttgcg gtggaagaaa tggtccgcgc ccggcaccac cactaccgtc aggtcctgcg
     1201 gccgcgccca ctcgagcacc gcggcgaggg ggatggtcgt atccagctcg ccatggatga
     1261 cgagcgtccc ggccggaacc ggcggcactt cgcgatcgac cactgcgacg ccgaccagga
     1321 cgagtctttc ggacgaaact ctctgcgcca gcctggcctg catcgccgcg ccgaacgaga
     1381 aacccgcaag caccatgggc tgcctgtcga tgcttttgta gacggcttcc agatcgtcga
     1441 cctcaccgcg gcctgcatcg tgcttgccct cgctttgccc gaccccgcgg aagttcggcc
     1501 gccaggcctc gtagcccagg tcgacgaagg tgcgcgccag ggtctgcgcc accttgttgt
     1561 cgagcgtgcc gccgaacagg ggatgcgggt gagcgacgag cgccgcgccg cgcgcaggtg
     1621 aatcgggcgc gtcgaccgcg cactcgatgc gtccagcggg gccggcgacg aactcgcgcc
     1681 gggtgctcgc cctcacatgc gcagccgctc gacgacgcgg ccgtgctgca ggtgctcctc
     1741 gatgatttcg tcgatgtcgg cgcggtcgac gtaggtgtac cagaccgcgt cggggtacac
     1801 gaccacgcag ggtccttcct cgcaacggtc gaggcagccc gcctggttga tcctgacctt
     1861 gccttctccg gcaaggccct tttccttcac gcgcttcttc gcgtagtcgc gcatctcgga
     1921 tgcgcccttg gcattgcagc aggggcggtc gccgccgtcg cgctggttgc agcagaagaa
     1981 gacgtgcttc tcgtaataag acatccgtcg attttacctt ctgcgtgcgg cgagaaagat
     2041 gagatacccc agagcggcga agggccatag cgcgcctacc aggcgcgtga ggccgttgaa
     2101 gttgaggaag gtcccctgct gccagacctt cagcgttgcg gcgaggtaag gattgggcgg
     2161 cgccaggttg acgaaaacag tggccgccat caccagtacg gcagcgagca cgaggcgcac
     2221 cgtgcgcggc agcgctaccg cggcgagtgc aacggccagg ccggcggcta gtcccgcctg
     2281 cgccccggga gtaaaccagg cgaacgcttc cgattgcatc atgagcgcga aggcgatcgc
     2341 cttgacggcg agcgcggcga ccacaaacgc cacgaacatc gctcgcaggc gctgcccggg
     2401 cgcgaccagc agggaggcga gcaggccgac cgccaccagg ttgaacgcgg cgatgaacgc
     2461 ttcggtgcgg atgaagaatt ccgcggcgtg ggcgcgcgcg gcgaccggtg cgatcagcgg
     2521 ccgcaggtcg cccgcgccga aaagcagcgt cgccggattg agctgcacga acagccacag
     2581 cgcgagcagc accaggccgg cgtcgatgtc gggaccgggc aggaacaggc gctcgcgcag
     2641 ccgcaggccg tccaggcgcg cgagcagggc cggcgcagcc gcggcgccga gcgccgcgcc
     2701 ggccacgttg cacgccatgt cgagattcga agcgaagcgc gccggcaggt agctctgcag
     2761 cgcctcgagc gagagcgaca gcaccagcgc cgccgccgtc gccgccgcag ccgcggcgcc
     2821 gccgcgcagc ctgggctgca gcgccgccac gcacaggaag ccgaagggcg cgtaggccag
     2881 cacgttgacc gccatgtcga acctgaggag ctggcgcggc cagtcctgca ggaggaaagc
     2941 gaacggcgaa gtgccgggat cgcgccagcc ctcgagcggg tagagcgacg cgtacgcgat
     3001 gaggatcaca tagacggcaa acaggatgcg gacgagcgcc gcagcgctcg gctgctgctc
     3061 aaccctgggg gcgtcgctca gccgtgcccg ccgtgcgcgg actgtgcctg ggtctgcccg
     3121 cgcggcacga gcgagcccgc gaccatgccg atggcggaga acaccaggcc gacgagcggc
     3181 gagggaagcg tcgggtcggt ggccagcgct tccaccgcgc tccacgccgc caggccaccc
     3241 agcgacgacg cgatggcgcc ggtattggtg gcgcgcttcc agaagatgcc ggcggcgagc
     3301 ggcacgaagg ccgccaccag cgtcaccgtg tacgcgttct ggatcatgtc gtacatggtc
     3361 ttcttcgaga gcagcgcgaa gaccagcgcg gcgaccgtga agcccaccag gatggcgcgc
     3421 agcgtggcga ggaactgccg gtcggtcatg cgcggaaaga actggcggac cacgttttcg
     3481 gtgatcagcg aggtcggcgc aatgatggcg ccgctcgcgg tcgacaggat cgccgagagc
     3541 agggcgccga aaaacaggat ctgtatccac atcggcgtgt ggccgaggat gaaggcaggc
     3601 aggatgcttt gcatctcgcg cgcgtcctct gccgagagca gcctgccgag cgaaggatcg
     3661 atgatcagtg cggcgtagac gatgaagatc ggcacgaagg cgaagctgaa gtagatcacc
     3721 ccgcccgcga gcgtgccgag aatcgcggtc ttctcgttct tcgccgaggt gacgcgctgg
     3781 aagatgtcct gctgcgggat cgatccgatc gccagcgtga ggaaggcggt gacgaaggcg
     3841 agccattcct tggcaccgcc cttgggccag aattcgaact ttcctccctc ggcggccgcg
     3901 gcgatgactt tgccgggccc gcccgccatg tcgccgacga accaggcgat gatcccaagg
     3961 ccgaggatga tcatcacgga ctggaacagg tcggtcatcg cgaccgacca catcccgccc
     4021 cagatggtgt atgcgaggac gaccacgccg ctgat
//
LOCUS       DASYJA010000295         2833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1405619, whole genome shotgun sequence.
ACCESSION   DASYJA010000295 DASYJA010000000
VERSION     DASYJA010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405619"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VGO02_06430"
     CDS             complement(<1..714)
                     /locus_tag="VGO02_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV7477356.1"
                     /translation="MTQKRVGIIMHGVTGRMGMNQHLIRSILEIRKQGGVALKSGERL
                     MPDPILVGRNAAKVEELAKKHGVERWTTDLGKALQSKDEIFFDAASTQLRPKLIKRAI
                     AAGKHVYCEKPVSTTLAEAKDLYAAAKKARIKHGVVQDKLWLPGLLKLKKLREQGFFG
                     KLLSVRGEFGYWVFEGDRVPAQRPSWNYRKQDGGGIILDMLCHWRYVLDNLCGSVRAV
                     SCRGATHIPYRHDEAGRKYK"
     gene            complement(711..2117)
                     /locus_tag="VGO02_06435"
     CDS             complement(711..2117)
                     /locus_tag="VGO02_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEV7477357.1"
                     /translation="MRFSIKEILFFERQVRLRLPFRFGVVTMTEAPQAFVRARIALEN
                     GKQAEGGAAEMLAPKWFDKDPALSNEQNFEQLRVSMRLARDAYLGGAENDAFGHSIEV
                     YGPQLALGTLQGLYNLTAGFGPALLDRALLDALCRALGVSFYDAVRKNVPGITAPGWQ
                     QDLATLDMNGFLFELRPAAIMAARHTVGMVDPITAADVKEKVNDGLPQTLEEVVARYG
                     HRYFKLKVSGDVPADVERLAGIASVLDKSEAAYQATLDGNEQFGSADEVIELWRKLRG
                     DPRLKRLTSSILFIEQPIKRSRALADDVSELAEIAKPLIIDESEDTLEAFPRARRLGY
                     TGVSSKTCKGFYKSILNLARCAMWNREVGSERYFMSGEDLTIQAGLALQQDLALTCLL
                     GLKHVERNGHHYVNGMAGLPEQEQAAFWTKHPDIYERSHGAVRLKIAEGVIRCKSLGC
                     PGFATETLPDWKSMKEFS"
     gene            complement(2117..>2833)
                     /locus_tag="VGO02_06440"
     CDS             complement(2117..>2833)
                     /locus_tag="VGO02_06440"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7477358.1"
                     /translation="PTEACLDINLDVRQSEFVAIVGPSGCGKTTILKMVAGLVPHTAG
                     SITVGGKAVDRPLTDVGIVFQESILLDWRSVLSNVMLQIDIRGKDAATGERDARQLLQ
                     ATGLSGFENKKPYELSGGMRQRVSICRALVHDPPLLLMDEPFGALDALTREQISMDIQ
                     RIWMEKRKTAIHITHSIPEAVLLADRVVVMGPRPGRIVEIIEIDLPRPRRLDKLPARF
                     NDYAGRIREIFKAKGVLAMD"
BASE COUNT          439 a          948 c          922 g          524 t
ORIGIN      
        1 cttgtacttc ctgccggctt cgtcgtgccg gtagggaata tgcgtcgcgc cgcggcagct
       61 cacggcgcgc acgctgccgc agaggttgtc gagcacgtag cgccagtggc acagcatgtc
      121 gaggatgatc ccgccgccgt cctgcttgcg gtagttccat gacgggcgct gcgccggcac
      181 ccggtcgccc tcgaagaccc aatagccgaa ttcgcctctt acggacagca atttcccgaa
      241 aaaaccctgc tcgcgaagct tcttcagctt gagcagcccc ggcagccaca gcttgtcctg
      301 caccacgccg tgcttgatgc gcgccttctt cgccgcggcg tacaggtcct tcgcttcggc
      361 aagcgtggtc gagaccggct tctcgcagta aacgtgcttg ccggcggcaa tggcgcgctt
      421 gatcagcttc gggcgcagct gcgtcgaggc ggcatcgaaa aagatttcat ccttcgattg
      481 cagcgctttt ccaagatcgg tggtccagcg ctcgacaccg tgcttcttcg ccagctcctc
      541 gaccttggcc gcgttgcggc cgaccaggat cgggtcgggc atcaggcgct cgccgctctt
      601 cagagcgacg ccgccctgct tgcggatctc caggatggac cggatcaggt gctggttcat
      661 tcccatccgt cctgtgacgc cgtgcatgat gatgccgacg cgcttctgcg tcatgaaaac
      721 tccttcatcg atttccagtc cggcagcgtt tcggtggcga agccggggca gccgaggctc
      781 ttgcagcgga tcacgccctc ggcgatcttg aggcggacgg caccatggct gcgctcgtag
      841 atgtcggggt gcttggtcca gaacgccgcc tgctcctgct cgggcagccc ggccatgccg
      901 ttcacgtagt ggtgtccgtt tcgctcgacg tgcttcaggc cgagcaggca ggtcagcgcg
      961 aggtcctgct gcagggcgag cccggcctgg atcgtgaggt cttcgcccga catgaagtag
     1021 cgctccgagc cgacctcgcg attccacatg gcgcagcgcg ccaggttgag gatcgacttg
     1081 tagaagccct tgcaggtctt cgacgacacg ccggtatagc ccaggcgccg cgcgcgcggg
     1141 aacgcctcca gcgtgtcctc cgactcgtcg atgatcagcg gcttcgctat ctcggcgagc
     1201 tcggaaacgt cgtcggccag cgcccggctg cgcttgatcg gctgctcgat gaacaggatg
     1261 ctcgaggtga ggcgcttcag gcgggggtcg ccccgcagct tgcgccatag ctcgatcacc
     1321 tcgtcggcgc tgccgaactg ctcgttgccg tcgagcgtcg cctggtaggc ggcctcgctc
     1381 ttatcgagca ccgaggcgat cccggccaga cgctcgacgt cggccggcac gtcgccgctc
     1441 actttgagct tgaaatagcg gtggccgtag cgcgccacca cttcctcgag ggtctgcggc
     1501 aggccgtcgt tcaccttttc cttcacgtcg gccgcggtga tcgggtcgac catgccgacg
     1561 gtgtgccgag ccgccatgat cgccgccggc cggagctcga acaggaatcc gttcatgtcg
     1621 agcgtcgcca ggtcctgctg ccagcccggc gcggtgatgc cgggaacgtt cttgcgcacc
     1681 gcgtcgtaga acgaaacgcc gagcgcgcgg cacaacgcgt cgagcaaagc gcggtcgagg
     1741 agcgccgggc cgaagcctgc ggtgaggttg tacaacccct gtagcgtgcc gagcgcgagc
     1801 tgcggcccgt atacctcgat cgagtggccg aaggcgtcgt tctcggcgcc gccgaggtag
     1861 gcgtcgcgcg cgaggcgcat cgagacgcgc agctgctcaa agttctgctc gttggacagc
     1921 gccggatcct tgtcgaacca cttgggagcc agcatctccg cggcgccgcc ctccgcctgc
     1981 ttgccgttct cgagcgcgat gcgcgcgcgc acgaacgcct gcggcgcctc ggtcatggtg
     2041 accacgccga agcggaacgg caggcgcagg cgcacctgcc tttcaaaaaa gagtatttcc
     2101 tttatcgaaa agcgcatcag tccatcgcca gaacgccctt ggccttgaag atctcgcgga
     2161 tgcgcccggc gtagtcgttg aagcgcgccg gcagcttgtc caggcgccgc ggccgcggca
     2221 ggtcgatctc gatgatctcg acgatgcgcc cgggacgcgg ccccatcacc accacccggt
     2281 cggcgagcag caccgcctcc gggatgctgt gggtgatgtg gatcgcggtc ttcctctttt
     2341 ccatccagat gcgctggata tccatcgaga tctgctcgcg ggtcagcgcg tcgagcgcgc
     2401 cgaagggctc gtccatgagc agcagcggcg gatcgtgcac cagcgcccgg cagatcgaca
     2461 cgcgctggcg catgccgccg gacagctcgt agggcttctt gttctcgaaa ccggaaagcc
     2521 ccgtcgcctg cagcagctgg cgagcgtccc gctcgccggt tgctgcatcc tttcctctga
     2581 tgtcgatctg cagcatgacg ttcgacagga cgctgcgcca gtcgagcagg atcgattcct
     2641 ggaacacgat gccgacgtcg gtgagcggcc ggtccactgc cttgccgccg accgtgatcg
     2701 agccggcggt gtgcggcacc aggccggcca ccatcttgag gatggtggtc ttgccgcagc
     2761 cgctgggacc gacgatggcg acgaactcgg attgccggac gtccaggttg atatcgaggc
     2821 aggcttcggt cgg
//
LOCUS       DASYJA010000296         4715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1223797, whole genome shotgun sequence.
ACCESSION   DASYJA010000296 DASYJA010000000
VERSION     DASYJA010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223797"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VGO02_06445"
     CDS             complement(<1..779)
                     /locus_tag="VGO02_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV7477359.1"
                     /translation="MMKPRGLGRGLDALLGSDTAAPRETLATVPVARIRPGKYQPRTR
                     MDQQALAELASSIRSQGLMQPLLVRPVDRERYELIAGERRWRAAQMAGLAEVPVLVRD
                     VPDEAALALSLIENIQRENLNPMEEAAGIQRLIDEFKMTHEQAADAVGRSRTATTNLL
                     RLLKLARPVQEMVMEGALDMGHARALLALDPARQIEAAHRIAAKGLSVREAESLAQSS
                     ARGKSAQRGSAKRDRDLERLEEELSASLGTTVEIHAAKKGRG"
     gene            complement(776..1546)
                     /locus_tag="VGO02_06450"
     CDS             complement(776..1546)
                     /locus_tag="VGO02_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7477360.1"
                     /translation="MARALAVVNQKGGVGKTTTSVNLAAALAQAERRVLLVDLDPQGN
                     ATMGSGVDKRNLARTVYHLLLGLAELQGVRIRVERGGFDLLPANRDLAGAEIELVELD
                     GRETRLRDALGRVDAEYDFVLIDCPPSLSLLTVNALTAAQRVLIPMQCEYYALEGLSD
                     LVATIKRVRANLNPQLEIAGLLRTMYDPRNTLSQHVSQQLEQHFGDKVYRTLVPRNVR
                     LAEAPSYGVPAVLWDAASKGAQAYLALAAEILAQGQAA"
     gene            complement(1548..2156)
                     /gene="rsmG"
                     /locus_tag="VGO02_06455"
     CDS             complement(1548..2156)
                     /gene="rsmG"
                     /locus_tag="VGO02_06455"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEV7477361.1"
                     /translation="MTPQDTLHRGVAELRLELPPGAEALLVRYMALLEKWNRTYNLTA
                     IREPAEVVSHHLLDCLAVVPHLPLPVQGTLADVGAGAGLPGIPIALARPQWRVTLNDS
                     SEKKVAFLRQAAIELELRNVAVHAGRVEQWTPQPKFALVISRAFAGLEQFRSRCAHLV
                     ASGGVLAAMTGAAPQADDCRVIALHVPLLEAQRHLVLCPAGS"
     gene            complement(2153..3991)
                     /gene="mnmG"
                     /locus_tag="VGO02_06460"
     CDS             complement(2153..3991)
                     /gene="mnmG"
                     /locus_tag="VGO02_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEV7477362.1"
                     /translation="MEFPQRFDVIVVGGGHAGTEAALAAARMGCRTVLLTHSVETLGQ
                     MSCNPSIGGIGKGHLVKEVDALGGAMALATDEAGIQFRILNSSKGAAVRATRAQADRL
                     LYKHAIRRRLENQPGLTLFQQPVDDIVLEGERVAGVVTQLGLQFRAPAVVLTTGTFLA
                     GLIHVGLSHHQAGRAGEPAAVTLAARLRELKLPVGRLKTGTPPRLDARSIDFSAMALQ
                     PGDSPEPVFSFIGARAMHPRQLPCWVTHTNERTHEVIRAGLDRSPMFTGVIQGVGPRY
                     CPSIEDKIHRFAGKASHQIFLEPESLSTNEIYPNGISTSLPFDVQLALVRSIRGLENA
                     HIVRPGYAIEYDYFDPRSLRDSLETKSIQGLFFAGQINGTTGYEEAAAQGLLAGINAA
                     NRVLGREPWSPRRDQAYIGVLVDDLVTRGVSEPYRMFTSRAEYRLMLREDNADLRLTE
                     TGRRLGLIDDARWEAFCRKRNAIGAETQRLRTTYVSTGFHEKRSIYELLRRPNESYAK
                     LVPASELDTAVVEQIEIQAKYEGYIERQRAEVERNAEMEAKLLPAELDYCQVKGLSAE
                     VQQKLNRHQPRTIGQAARISGVTPAAISLLLVHLKRGFRSAEHKTA"
     gene            4133..>4715
                     /locus_tag="VGO02_06465"
     CDS             4133..>4715
                     /locus_tag="VGO02_06465"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7477363.1"
                     /translation="MISLARYAALLEPRDMKQTIAASMIGRLPIGITGLAILLLVQAA
                     TDSFSKGGAATACYVIGLATVAPALGRLIDRNGPRLALLACSIAFPAALLSLVAAVET
                     RSHPALILVSAAAAGVTFPPITVCMRTYFKQRLSDDALLAAAYSLESVLIELIFIVGP
                     MLVALFVAAASPAAAVLFAAASGCVGTLLFLRSP"
BASE COUNT          729 a         1609 c         1536 g          841 t
ORIGIN      
        1 ccgcggccct tcttcgccgc atggatctcg accgtggtgc cgagactcgc ggagagctcc
       61 tcttccagcc gctcgaggtc gcggtcgcgc ttggccgagc ccctttgggc cgacttgccg
      121 cgcgcgctcg attgcgccag ggactcggcc tcgcgcaccg agaggccctt cgcggcgatg
      181 cggtgcgcgg cttcgatctg gcgcgcggga tcgagcgcca gcagcgcgcg cgcgtgtccc
      241 atgtcgagcg cgccctccat caccatctcc tgcaccggcc gcgcgagctt cagcaggcgc
      301 aacaggttgg tggtggcggt gcgcgagcgc ccgaccgcgt cggctgcctg ctcgtgcgtc
      361 atcttgaact cgtcgatcag ccgctggatg cccgccgcct cctccatcgg attgaggttc
      421 tcgcgctgga tgttttcgat cagcgagagg gcgagggccg cttcgtcggg gacgtcgcgg
      481 accagcaccg gcacctccgc gagtcccgcc atctgcgccg cgcgccaccg gcgctcgccc
      541 gcgatcagct cgtagcgctc gcggtcgacg ggcctgacga gcagcggctg catcagcccc
      601 tgggagcgga tcgaagacgc cagctcggcg agcgcctgtt ggtccatcct ggtgcgcggc
      661 tggtattttc cgggccggat gcgtgcaacc ggaactgtcg caagcgtctc tcgcggcgca
      721 gcagtgtccg atccgagcag cgcgtcgagg ccacggccga gtcccctggg tttcatcatg
      781 cggcttgtcc ctgagcgaga atttccgcgg ccagcgcgag ataggcctgc gcccccttgg
      841 aggccgcgtc ccacagcacc gcgggcacgc cgtagctcgg cgcttccgca agccgcacgt
      901 tgcgcggcac cagggtgcga tacaccttgt cgccgaagtg ctgctcgagc tgctgcgata
      961 cgtgctgcga cagcgtgttc ctggggtcgt acatggtgcg cagcaggccc gcgatttcga
     1021 gctgcggatt gaggttcgcg cgcacgcgct tgatcgtggc gacgaggtcc gacagcccct
     1081 cgagcgcgta gtactcgcat tgcatcggaa tgagcacccg ctgcgccgcg gtcagcgcgt
     1141 tcaccgtgag caggctgagc gacggcgggc aatcgatgag cacgaagtcg tattccgcgt
     1201 cgacccgccc cagcgcatcc ctcagccggg tctcgcgccc gtcgagttcc accagctcga
     1261 tttccgcccc cgcgaggtcg cgattggcag gaagcaggtc gaagccgcca cgctcgacgc
     1321 gtatgcgtac cccctgcaac tccgccaagc ccaggagcag gtggtagacc gtgcgggcga
     1381 gattgcgctt gtcgacgccg ctgcccatgg tcgcattgcc ctgtggatcc aggtcgacca
     1441 gcagcactcg gcgctcggcc tgagcgagcg ctgccgccag attgacgctg gtggtggttt
     1501 tgcccacgcc gcctttctgg ttcaccaccg cgagcgcgcg agccatctca gctcccggcc
     1561 gggcaaagca cgagatgacg ctgcgcctcg agcagcggca cgtggagcgc aatgacccgg
     1621 cagtcgtcgg cctgcggcgc ggcgccggtc atcgctgcca gcacgccgcc ggaggccacg
     1681 aggtgcgcgc aacgcgacct gaactgctcc agcccggcaa acgcgcggga aatgacgagc
     1741 gcgaatttcg gctgcggcgt ccattgttcg acgcgccccg catgcacggc aacattccgc
     1801 agctcgagct cgatggccgc ctggcgcaga aaagcaacct tcttctcgct cgagtcgttc
     1861 agggtgacgc gccattgcgg ccgcgcgagc gcgatgggga tgcccggcaa gccggcgccc
     1921 gcccccacgt cggcgagcgt gccctgcaca ggaagcggaa ggtgggggac cacggcaagg
     1981 cagtcgagca ggtggtggct cacgacttca gcgggctcgc ggattgccgt caggttgtag
     2041 gtgcggttcc atttttccag cagcgccatg tatcgcacga gcagcgcctc ggcgcccggc
     2101 ggcagctcga ggcggagctc ggccacgccg cggtgaagcg tgtcctgcgg cgtcatgccg
     2161 tcttgtgctc ggcagatctg aagccccgct tcagatgcac gagcagcagc gagatcgccg
     2221 ccggcgtgac acccgagata cgcgccgcct gcccgatggt gcggggctga tggcggttga
     2281 gcttctgctg gacctccgcc gagaggccct tcacctggca gtagtcgagc tccgcgggca
     2341 gcagcttcgc ttccatttcg gcattgcgct ccacttcggc gcgctggcgc tcgatgtagc
     2401 cctcgtactt cgcctgtatt tcgatctgct cgacgaccgc ggtatcgagc tccgacgcgg
     2461 gaacaagctt cgcgtacgat tcgttcggtc ggcggagaag ctcgtaaatt gagcgctttt
     2521 cgtgaaagcc tgtgcttaca tacgtcgtcc gcagacgctg cgtttccgcg ccgatggcgt
     2581 tgcgcttgcg gcagaacgcc tcccagcgtg cgtcgtcgat caagcccagc cggcgaccgg
     2641 tttcggtgag ccgcaaatcc gcgttgtcct cgcgcagcat aaggcggtat tcggcgcggc
     2701 tggtaaacat gcggtacggt tcggatactc cgcgggtcac caagtcgtcg acgagcactc
     2761 cgatgtacgc ctgatcgcgc cgcggcgacc acggctcccg gccaagcacg cggttcgcgg
     2821 cgttgattcc cgcgagcaag ccctgcgcag ccgcttcctc gtagccggtc gttccgttga
     2881 tctgtcccgc gaagaacagg ccctggatgc tcttcgtttc gagcgagtcg cgcagactcc
     2941 gcggatcgaa gtagtcgtac tcgatcgcgt agcccgggcg cacgatatgc gcgttttcga
     3001 gtccccggat tgaccgcacg agcgcgagtt gcacgtcgaa aggcaggctc gtggaaatgc
     3061 cgttcgggta gatctcgttc gtcgacagac tttcgggctc cagaaaaatc tggtggctcg
     3121 ccttgcccgc aaaacggtgg atcttgtctt cgatcgaggg acagtaacgg gggcccacgc
     3181 cttgaatgac gcccgtaaac atcggcgagc ggtcgagtcc cgcacggatg acctcgtggg
     3241 tgcgctcgtt cgtgtgggtc acccaacacg gcagctggcg cggatgcatg gcacgagcgc
     3301 cgatgaaaga aaacaccggc tcaggcgaat cgccaggctg cagcgccatc gccgagaaat
     3361 cgatgctgcg cgcgtccagg cgaggcggcg ttcccgtttt gagccgtccg accggcaact
     3421 tcaactcccg cagccgcgcc gccagcgtga cggcggcggg ctcaccagcg cgtcccgctt
     3481 gatggtggct caaaccgacg tggatcaagc cggccaggaa agtgccggtc gtcagcacga
     3541 ccgcgggtgc ccggaattgc aacccaagct gcgtcacgac gccggccact cgctcgccct
     3601 cgagcacgat atcgtccacg ggctgctgga agagcgttag accgggctgg ttttcgagtc
     3661 ggcggcggat cgcgtgctta tagagcagcc ggtcggcttg ggcacgggtg gcacggacag
     3721 cggcaccttt ggacgaattc aggatccgga actggatgcc cgcttcatcc gtggccaaag
     3781 ccattgcgcc gcccagcgca tccacttcct tcaccagatg cccctttccg atacccccga
     3841 tagaagggtt gcaggacatc tgcccgagcg tttccacgct atgagtcagc aggacggtcc
     3901 ggcagcccat gcgcgcggca gcgagggcgg cctcggtacc agcgtggccg cctccgacca
     3961 cgatcacgtc gaatctctga gggaattcca tccggaggcg gggcgaattt tacgcgatgt
     4021 tccacgtgga accagcgctc gcgccaacgc cgttggttcc acgtgaaaca tcgcgtcgtg
     4081 ctgcatcgca gcaaagagat gggatgcgac aatcgatcgc ccccggctcc tcgtgatttc
     4141 cctcgcccgc tacgccgcgc tgctcgagcc gcgcgacatg aagcagacta ttgccgcgtc
     4201 catgatcggc cggctgccga tcggcatcac cgggcttgcg atcctgctgc tggtgcaggc
     4261 ggcgaccgat tccttctcaa agggcggggc agccactgcc tgctacgtca ttgggcttgc
     4321 caccgtcgcg ccagcgctgg gtcgtctcat cgatcgaaac gggccacggc tcgcattgct
     4381 ggcatgcagc atcgccttcc ctgcagcact tctaagcttg gtggcggccg ttgaaacgcg
     4441 ctctcacccc gccctgatcc tggtctccgc tgccgcagcg ggcgttacgt tcccgccgat
     4501 caccgtgtgc atgcgcacgt acttcaagca gcgcctgagc gacgacgcgc tgctcgctgc
     4561 ggcctattct ctcgaatccg tgctgatcga actgatcttc atcgttgggc cgatgctcgt
     4621 cgccctcttc gtggctgcgg cttcgcccgc cgctgcagtc cttttcgcgg ccgcctcggg
     4681 gtgcgtcggc acgctgctct ttctgcgctc gccac
//
LOCUS       DASYJA010000297         4393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1860433, whole genome shotgun sequence.
ACCESSION   DASYJA010000297 DASYJA010000000
VERSION     DASYJA010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860433"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="VGO02_06470"
     CDS             complement(<1..1233)
                     /locus_tag="VGO02_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HEV7477364.1"
                     /translation="MKFSILCSAVAAAFAVGAQAAQQETSSTLADQQSVAVTIYNENL
                     ALIKDTRRVALDAGLNRLALREVSGRMRPETASLRSLTHPGSLSLIEQNFDFDLLTPA
                     KLLEKYVGREVRVFRVNQKTGVESFETATVLAANGGVVLKIGDRIETGLPGRIVYDGV
                     PPNLRDRPTLVTELQSARAGNQTVELSYLSGGLSWKADYVAELNAGDSALDLNGWVTL
                     TNTSGTAYPNARLQLVAGDVNRVRDELRLVAKSSGMARAEAAAPRQMTQESLFEYHLY
                     TLQRPTTIADSQTKQVALLSAAAVPVKKELVLQGSDYYYRSSVGGIGQKMKVGVFVQF
                     DNREAANLGMPMPKGVVRVYKKDGAGNAQFVGEDSIDHTPKNENVRLKLGEAFDVTGD
                     KKQTDFKRREPTNQASYVF"
     gene            complement(1290..2147)
                     /locus_tag="VGO02_06475"
     CDS             complement(1290..2147)
                     /locus_tag="VGO02_06475"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV7477365.1"
                     /translation="MRTYDAGRWLLLATLWSVQYLFLRIAVPVFGTAVSAEARALFAA
                     LFVVPWVIYFARLPIAPFRCWKDHLAVGLVNNVLPFACMAWAATVLPAGYLAIINGMV
                     PLWAALSAAWFLKEPLGAHRAAGFLLGIAGVALLVNLGPVELEAKTVAAACVAVLGAA
                     FWGWAGVIIKQRSGNLPPMGLAAGSIAWAAVLMAPAWVAAPPPQAWTLEATAALIAVG
                     ALCSGLAYLPFFTLIRDIGPTRTLSVGLLVPVLGVLWGWLLLGEAVTFAMLACAGLVV
                     AALFLVMRR"
     gene            complement(2148..3131)
                     /locus_tag="VGO02_06480"
     CDS             complement(2148..3131)
                     /locus_tag="VGO02_06480"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV7477366.1"
                     /translation="MEQRKLGRSPLQVSALGLGLMSMSGVYGNADDGQSISVIHHALE
                     RGVNFLDSADMYGWGHNEELLGKALKGRRDRAIVATKFGQTKNAEGKQGVDGSPAYVQ
                     RACEASLKRLGIDTIDLYFQHRVDPETPIEDTVGAMKRLIEQGKVRALGLSEAAPETI
                     RRANKVHPIAAVQNEYSLLFRKEGEETLLVTRELGISLIAYAPLGRSMLTGTVHGKAD
                     LPEGDRRLAHPRFQGDSLEKNVSLVMKLEAIAKEKRCTPPQLVLAWLLAQGKDIVPIP
                     GTKRKERFDENLASLKIELTEKDLKRISEAAPAGAGAGTRYPADAMKRVYR"
     gene            complement(3143..3901)
                     /locus_tag="VGO02_06485"
     CDS             complement(3143..3901)
                     /locus_tag="VGO02_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477367.1"
                     /translation="MSSVETELQVLYKIGNAAVREYPYPHIYVPDIFPADFYAELRRN
                     LPPQSAMKNLGELGRVIGTTYPARGVLPLMQKNLAALDEAQRGFWERLGNWLLGRRFA
                     DIMISKFAPYLAQRFGDLRSVRFSAEALIVRDRSTYTLGPHTDAPSKALSFLFYLPPD
                     GSMAHLGTSIYLPRDPSFMCPGGPHHPFDQFERMLTMPYVANALFAFMKTPNSFHGVE
                     PIEEAGVQRDLLLYDIRAEVAQQGATQQGAKFTF"
     gene            complement(3882..>4393)
                     /locus_tag="VGO02_06490"
     CDS             complement(3882..>4393)
                     /locus_tag="VGO02_06490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEV7477368.1"
                     /translation="PEAQNYGLLNRNLLMNGLGEQVKAYCLALSDTAGFSELHLATLD
                     AGGSCHQLGEKVDFNHRPAQPRFSQGCLSARLDDLVRDGVVPPPQHIKIDVDGFEPKV
                     IAGAAQVLRAPTTRSLLIEVNQNLPDHMAMVLELNQLGFAHDPAQVARAERKDGTFKG
                     CAEYVFRRD"
BASE COUNT          683 a         1436 c         1506 g          768 t
ORIGIN      
        1 gaagacgtag ctcgcctggt tggtcggctc gcgccgcttg aagtcggtct gcttcttgtc
       61 gccggtgacg tcgaaggcct cgccgagctt caggcgcacg ttctcgttct tcggcgtgtg
      121 gtcgatgctg tcttcgccga cgaattgcgc gttgccggcg ccgtccttct tgtagacgcg
      181 caccacgccc ttcggcatcg gcatgccgag attcgcggct tcgcggttgt cgaactgcac
      241 gaacacgccg accttcatct tctggccgat gccgccgacg ctggaccggt agtaatagtc
      301 gctgccctgc aggaccagtt ctttcttcac cggcacagcg gctgcgctga gcagggccac
      361 ctgcttggtc tggctgtcgg cgatggtggt cggacgctgc agcgtgtaga ggtggtactc
      421 gaacagcgac tcctgcgtca tctgccgcgg cgcggccgcc tcggcccgcg ccatgccgga
      481 ggacttggcg accaggcgca gctcgtcgcg cacgcggttg acgtcgccgg cgacaagctg
      541 caggcgcgcg ttcgggtagg cggtgccgct ggtgttggtg agggtgaccc agccgttcag
      601 gtcgagggcg ctgtcgcccg cgttcagctc ggcgacgtag tcggccttcc aggaaaggcc
      661 gcccgacagg tacgagagct cgacggtctg gttgcccgcg cgcgcgcttt gcagctcggt
      721 caccagcgtg ggccggtcgc gcaggttggg cggcacgccg tcgtagacga tgcgcccggg
      781 caggccggtc tcgatgcggt cgccgatctt gagcaccacg ccgccgttgg cggcgaggac
      841 ggttgcggtc tcgaacgatt ccacgcctgt tttctgattg actctgaaaa ccctgacttc
      901 ccggccgaca tatttctcga gcagcttggc cggggtgagc aggtcgaagt cgaagttctg
      961 ctcgatgagc gaaagcgacc cggggtgcgt caggctgcgc agcgacgccg tctccggccg
     1021 catgcgcccg ctcacctcgc gcagcgccag gcggttcagc ccggcgtcga gcgccacgcg
     1081 tcgcgtgtcc ttgatcaggg cgaggttttc gttgtagatc gtgacagcga ccgactgctg
     1141 atccgcgagc gtgctcgacg tttcctgctg ggccgcctgc gcgccgaccg cgaacgccgc
     1201 ggctacggca gaacacaata ttgagaattt cactttcgcc tcccggttgt tggtagcctc
     1261 cctttaaacg ggggactcct cgggaagggt taacgccgca tcaccaggaa gagggctgcc
     1321 acgaccaggc cggcgcaggc cagcatggca aaagtcacgg cctcgccgag cagcagccag
     1381 ccccacagga cgccgagcac cggcacgagg agcccgaccg agagggtgcg cgtcggacca
     1441 atgtcgcgga tcagggtgaa gaagggcagg taggccagcc cgctgcacaa cgcgccgacg
     1501 gcgatcagcg ccgcggtggc ttccagggtc caggcctgcg gcggcggtgc ggcgacccag
     1561 gccggcgcca tcagcaccgc cgcccaggcg atcgagcccg ccgcgaggcc catcggcggc
     1621 aggttgcccg agcgctgctt gatgatcacg ccggcccagc cccagaaggc cgcaccgagc
     1681 acggcgacgc acgcggcggc gaccgtcttc gcttccagct cgaccggccc gaggttgacg
     1741 agcagcgcga cgccggcgat gccgaggagg aagcccgcgg cgcggtgcgc accgagcggc
     1801 tccttcagga accaggctgc ggagagagca gcccatagcg gcaccatgcc gttgatgatg
     1861 gcgaggtagc ccgccggcaa caccgttgcc gcccacgcca tgcaggcgaa gggcagcacg
     1921 ttgttgacca ggccgaccgc gaggtggtct ttccagcacc tgaaaggcgc gatcggcagg
     1981 cgcgcgaagt agatcaccca cgggacgacg aacagcgcgg cgaacagcgc ccgcgcctct
     2041 gccgacacgg ccgtgccgaa gacgggcacg gcgatcctca ggaaaagata ctgcaccgac
     2101 cagagggtgg cgagcagcag ccaccgcccg gcgtcgtagg ttcgcaccta ccggtagacg
     2161 cgtttcatcg catccgcggg gtagcgggtg cctgcgcccg cccctgcggg ggcggcttcg
     2221 gagattcttt tcagatcttt ttcagtcaat tcaattttta atgaagctag attctcgtca
     2281 aagcgttctt tgcgcttggt gccgggaatc ggcacgatgt ccttgccctg cgccagcagc
     2341 cacgcgagca ccagctgcgg cggcgtgcag cgcttctcct tggcgatagc ctcgagcttc
     2401 atgaccaggg aaacattctt ttccaaggaa tccccttgaa agcgcggatg cgccagacgg
     2461 cggtcgcctt cgggcaggtc ggccttgccg tgcaccgtgc cggtgagcat gctgcggccg
     2521 agcggcgcgt aggcgatcag cgagatgccg agctcacgcg tgaccagcag cgtttcttcc
     2581 ccttcttttc tgaatagcaa tgagtattcg ttctgcaccg ccgcgatggg atgcaccttg
     2641 ttggcgcggc ggatcgtctc gggcgcggcc tcggacaggc cgagggcgcg gaccttgccc
     2701 tgctcgatga gccgcttcat cgccccgacc gtgtcctcga tcggggtctc gggatcgacg
     2761 cggtgctgga aatagaggtc gatcgtgtcg atgcccaggc gcttgaggct cgcctcgcac
     2821 gcccgctgca catacgccgg gctgccgtcc actccttgct tgccctcggc gttcttcgtc
     2881 tggccgaact tggtcgccac gatcgcccgg tcgcgccgtc ccttgagcgc cttgccgagc
     2941 agctcctcgt tgtgccccca gccgtacatg tcggccgagt cgaggaagtt cacgccgcgc
     3001 tcgagcgcgt ggtggatgac cgagatcgac tggccgtcgt cggcgttgcc gtacaccccc
     3061 gacatcgaca tcaggcccag tccgagggcg gagacctgca gggggctgcg ccccagtttg
     3121 cgctgttcca tcgattgctt cctcagaaag tgaatttcgc gccctgctgc gtcgcgccct
     3181 gctgggccac ctcggcccgg atgtcgtaga gcagcaggtc gcgctgcaca cccgcttcct
     3241 cgatcggctc gacgccgtgg aaggagttcg gcgtcttcat gaaggcgaac agcgcgttcg
     3301 ccacgtacgg catggtcagc atccgctcga actggtcgaa tggatggtgc gggccgcccg
     3361 ggcacatgaa ggacgggtcg cgcggcaggt agatcgaggt gccgaggtgc gccatcgagc
     3421 cgtctggtgg caggtagaac aggaacgaca gcgctttgct tggcgcgtcc gtgtgtggtc
     3481 cgagcgtgta ggtgctgcgg tccctgacga tcagcgcctc ggcgctgaag cgcacgctgc
     3541 gcaggtcgcc gaagcgctgc gccaggtaag gcgcgaactt gctgatcatg atatcggcga
     3601 agcggcggcc gagcagccag ttgcccaggc gctcccagaa gccgcgctgc gcttcgtcca
     3661 gcgcggcgag attcttctgc atgagcggca gcacgccgcg cgccggatag gtagtgccga
     3721 tcacgcggcc gagctcgccg aggttcttca tggcgctttg cggcggcagg ttgcgccgca
     3781 gctcggcgta aaagtccgcg gggaagatat cgggcacata gatgtgcgga tagggatact
     3841 cccgcaccgc ggcgttgccg atcttgtaga ggacctggag ctcagtctcg acggaagaca
     3901 tattccgcgc agcctttgaa tgtgccgtcc ttgcgctcgg cccgcgccac ctgcgcgggg
     3961 tcgtgggcga agcccagctg gttgagctcg agcaccatcg ccatgtggtc gggcaggttc
     4021 tggttgacct cgatcagcag cgagcgcgtc gtcggcgcgc gcagcacctg cgccgcgccg
     4081 gcgatcacct tgggctcgaa gccgtcgacg tcgatcttga tgtgctgcgg cggcggcacg
     4141 acgccgtcgc gcaccaggtc gtcgagccgc gcggacaggc agccttgcga gaagcggggc
     4201 tgcgccggcc ggtggttgaa gtcgaccttc tcgcctagct ggtggcacga gccgccggcg
     4261 tcgagcgtgg cgaggtgcag ctcgctgaag cctgcggtgt cggacagcgc caggcagtag
     4321 gctttcacct gttcgccgag cccgttcatc agcaggttgc ggttgagcaa tccgtagttc
     4381 tgcgcctcgg gct
//
LOCUS       DASYJA010000298         1627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2494461, whole genome shotgun sequence.
ACCESSION   DASYJA010000298 DASYJA010000000
VERSION     DASYJA010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2494461"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VGO02_06495"
     CDS             <1..407
                     /locus_tag="VGO02_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV7477369.1"
                     /translation="ERQVLEEVTNARARATQVQVQIPPDIVNNPEAFKRFQEAQAQLT
                     GALGRLLAVSERYPDLKSNQNFLALQSQLEGTENRIAVARRDYIEAVRQYNTELRTFP
                     GIIWASTLYKNNKPMETFTVAEDVKTPPQVKF"
     gene            646..1302
                     /locus_tag="VGO02_06500"
     CDS             646..1302
                     /locus_tag="VGO02_06500"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEV7477370.1"
                     /translation="MVDEANILDPQTRAALTEKLAALEAKTTDQLVVVTLKSLQGTSI
                     EDFGVQLGRHWQIGQKDKNNGVLLIVAPNERKVRIEVGYGPEGALTDAVSRLIIENAI
                     TPRFRAGDFAGGITRGVDDITSVLSGDAEEWKARAAQRPDTVSPTGSTRDDSIWPVIW
                     VVLIVMGVVFFCAMLAGALCAAILRLLALLSLSGGRSSSGGFSGGGGSFGGGGASGGW
                     "
     gene            1341..>1627
                     /locus_tag="VGO02_06505"
     CDS             1341..>1627
                     /locus_tag="VGO02_06505"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEV7477371.1"
                     /translation="MFHRPASLPSFAGAALLALLSLVPALALDFPVLSGRVVDDAGIL
                     DAGTRAALTQKLAEFEAKTSDQLVVVTLKSLQGTSVEDFGVELGRHWQIGQ"
BASE COUNT          271 a          499 c          545 g          312 t
ORIGIN      
        1 aggagcgcca agtgctcgag gaagtgacca acgcgcgggc gcgcgcgacc caggtccagg
       61 tgcagattcc gccggatatc gtcaacaatc cggaagcatt caagcgcttc caggaggcgc
      121 aggcgcaact caccggcgcg ctcgggcgtt tgcttgcggt ttcggagcga tatcccgacc
      181 tcaaatcgaa ccagaacttc cttgccctgc agtcgcagct cgagggcacg gagaatcgca
      241 ttgcggtcgc acgccgcgac tacatcgagg cggtgcggca atacaacacg gagctacgca
      301 cgtttcccgg catcatctgg gcctcgactc tctacaagaa caacaagccg atggagacgt
      361 tcacggtggc ggaagacgtg aagacgccgc cgcaggtcaa attctgatcc tgcaacgtgc
      421 gcgatgagtg ccgctggaga cctccgagtg gcgccgcaga ccgcccccgg actcggtgtg
      481 ctccctctct caggcagccc gggcttgccc gacttacgct ggggcagggc ttggaagggt
      541 agttgtgccg ttgggagtcg gtggtggctc gcgcttgtcg tcgtcgtcgc attccttctt
      601 gtctttcccg cgctcgcgct cgacttccct gcgctcagcg gccgcgtggt ggacgaggct
      661 aatattctcg atccgcaaac gcgggcggct ctcaccgaaa aactcgccgc gctcgaagcc
      721 aagaccaccg accagctcgt ggtcgtgacg ctgaaatcgc tgcaaggaac gtcgattgag
      781 gatttcgggg tgcagctcgg tcgccattgg cagatcgggc agaaggacaa gaataacggc
      841 gtgttgctga tcgtggcgcc gaacgagcgc aaggttcgca tcgaagtggg ctacggaccc
      901 gagggcgcgc tgaccgatgc tgtctccagg ctcatcatcg agaatgcgat caccccgcgg
      961 tttcgcgccg gagacttcgc gggcggcatc acgcgcggcg tcgacgacat caccagcgtg
     1021 ctcagcggcg acgccgagga atggaaagcg cgcgcggcgc agcggccgga tacggtctcg
     1081 cccaccggct ccacgcgtga tgattcgatc tggccggtga tctgggtggt gctgatcgtc
     1141 atgggagtgg tcttcttctg tgccatgttg gcgggtgcgc tgtgcgcggc gatccttcgg
     1201 ttgcttgcat tgctgtcgct gtccggcggc aggagctctt cgggcgggtt ttccggcggc
     1261 ggcggctcgt tcggcggcgg cggcgcctcg gggggatggt gatgctcggg cgttatacgg
     1321 cctcttgcta cgagcgcggt gtgttccacc gccccgcaag tctcccgtcc ttcgcgggcg
     1381 cggcgttgct tgctttgcta tcgttggttc ccgcgctggc gctggatttt cccgtactta
     1441 gcggccgcgt ggtcgacgat gctggcattc tggatgcagg aacacgcgcg gcgctcaccc
     1501 aaaagctcgc cgagttcgaa gccaaaacca gcgaccagct cgtggtggta acgctcaaat
     1561 cgctgcaggg tacctcggtc gaggatttcg gcgtcgagct tggccgccac tggcagatcg
     1621 gtcagaa
//
LOCUS       DASYJA010000299        12609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1951127, whole genome shotgun sequence.
ACCESSION   DASYJA010000299 DASYJA010000000
VERSION     DASYJA010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12609
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1951127"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..263)
                     /locus_tag="VGO02_06510"
     CDS             complement(12..263)
                     /locus_tag="VGO02_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477372.1"
                     /translation="MISKTHLAKAVEHALSGKWREAHEIVQQDEQDETACWIHAVLHR
                     QEGDLPNAQYWYARCGRRLRKALTPDAELREIEAALQQG"
     gene            complement(238..723)
                     /gene="mug"
                     /locus_tag="VGO02_06515"
     CDS             complement(238..723)
                     /gene="mug"
                     /locus_tag="VGO02_06515"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HEV7477373.1"
                     /translation="MIAPGLRVLFCGINPGLYSGATGHHFARPGNRFWPALHQAGFTN
                     RPLHPSEQRELLKAGFGVVNLVNRATAAADELAPEEFVAGRRRLAAKIRRYRPKAVAF
                     LGVGAYCQAFGLRNASIGIQPDFFEGAKVWVLPNPSGLNANYQLPDLVRLFRDLQNAS
                     G"
     gene            complement(773..1573)
                     /locus_tag="VGO02_06520"
                     /pseudo
     CDS             complement(773..1573)
                     /locus_tag="VGO02_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004528634.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
     gene            complement(1548..4667)
                     /locus_tag="VGO02_06525"
     CDS             complement(1548..4667)
                     /locus_tag="VGO02_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV7477374.1"
                     /translation="MNFCEPFVRRPVMTVLLMAGLLIFGIAAYRLLPVSTLPNVDFPT
                     IQVTAELPGASPETMASAVATVLERQFSTIAGIDEMTSVSGQGVTRITIQFSLDRDID
                     AAAQDVNSAIASASRQLPATMSAPPSFRKVNPADFPVFFLALSSDALPIAVVNEYAET
                     NLAQRISTINGVAQVQVFGQQKYAVRIQVDPNALAARGVGINEVEQAVSQANVNLPTG
                     TLYGKDRVFAVQATGQLNDAAAFRPIIVTYRNGSPIRRQELGTVTDSVQNDKLGAWFK
                     GKRGIVLAIQRQPGTNTIEVVDNIKKLLPTFRAEVPPAINIDILYDRSQTIRASVQEV
                     QFTLYLALCLVVMVIFLFLRNVAATVIPAIALPLCIIGTFAVMYVFGYSLDNISLMAL
                     TLCVGFVVDDAIVMLENITRHLEQGKSVLQATLDGSREIVFTIISMTISLAAVFIPVL
                     FMGGILGRLLHEFAVTIITAVLLSGFVSLTLTPMMCSRILKPHSKDERHGRLYMAFER
                     AFDAWRDAYDASLRWTLGHQRLVLIAFLGVCIGTGVLFVRAPKGFLPSEDAGQLLCFV
                     EGPQDTSYEAMIELQSQVVEIIRKDPNVEATMAFVGANAGSPLLNIARVTVNLKPFKE
                     RKPADQIVRELRPKLANILGAKVFIQNIPSIRIGGQLTKSPYQYVMRSSSIEELYHWA
                     PTIEQKLRSLPGLIDVTSDLQITRPEVTVQIEREKASALGVSAQSIEMALNNAYGARQ
                     VSTIYTATNQFWVILELSPRYQTDPTVLQMLYVRSSTGALVPLNAVASLAYGVGPLQV
                     THLGQLTSVTYSFDLPPGVALSEAIDQIQRATREMQVPATLTGSFAGTAQAFQSSLQG
                     MGILILLSIFVIYLVLGILYESFIHPLTILSGLPTAGLGALLTLMAFGVELNMYAFVG
                     IIMLVGIVKKNAIMMIDFAIEAQRKDGMAPEQAIHQAGLVRFRPIMMTTMAALMGSLP
                     IAIGFGAGGGARMPLGLAVVGGLLLSQALTLYITPVIYLYFERLQDWLNKDKRRPEME
                     PRAGQPRSA"
     gene            complement(4664..5806)
                     /locus_tag="VGO02_06530"
     CDS             complement(4664..5806)
                     /locus_tag="VGO02_06530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV7477375.1"
                     /translation="MSKRLLFAAVLVAVLAAAGLGTYFVSDGRAKEGKRAGKGPPAVP
                     VNVAAAQQQTVPVRLTGIGNVEAYQTVSIKARVDGQIVEVNFREGDPVKKGVALFRID
                     PRPYQSTLRQAEANAMRDKAARDQARSQATRYQELLDKNFISKEAFAQIRTNAETAEA
                     VARSSQAAVENARLNLEYCTITSPLDGYVGKVLLQAGNLVKANDVGALVVINQVKPIY
                     VNFSVPEQNLPEVRKYMAGGPLSVEVVPSDPAVARIPGQLIFVDNAVDPTTGTIKLRA
                     QFPNEEAALWPGQFVNVSLRLYEQADAIIIPATAVQTGPEGQYVYVVGPDLLAELRKV
                     TVQRADGERAIIATGLAKGERVVTRGQLRLGPKTPVQIAKPAAEAS"
     gene            complement(5820..7067)
                     /gene="lysA"
                     /locus_tag="VGO02_06535"
     CDS             complement(5820..7067)
                     /gene="lysA"
                     /locus_tag="VGO02_06535"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV7477376.1"
                     /translation="MTFAYRNGVLCAEQVPLDDIARRFGTPCYVYSRAAIEGAYQEYA
                     RALQGRDSLVCYSVKANSNLAVLALLARLGAGFDIVSGGELARVLAAGGDARKTLFSG
                     VGKTQAEIQLALEKNILCLNLESESELARVSEVAGRLGRRAPVAFRVNPDIDARTHPY
                     ISTGLRETKFGIAYAEAERIYAAAARLPHIELVGIGCHIGSQLTRAAPFVEAAGRIAA
                     LADRLGRAGIALKHIDIGGGIGIRYRDETPEPIAGFLDGALGMLAGRRETLIVDPGRS
                     IVGNAGVLLSRVEYVKPGAARNFVVVDAAMNDLVRPALYEAWHEVRAVRQPEEGAAQT
                     VYDIVGPVCESGDFLAKERRLAARAGDLLVLMSAGAYGMVMSSNYNSRPRAAEVLVDG
                     AAAHLVRSRESVEQLFALERIPG"
     gene            complement(7070..7258)
                     /locus_tag="VGO02_06540"
     CDS             complement(7070..7258)
                     /locus_tag="VGO02_06540"
                     /inference="COORDINATES: protein motif:HMM:NF025012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEV7477377.1"
                     /translation="MRFLPLFIASGMVLALGAGCGQKGPLYLRDKPPPGMKPPKPAPV
                     KPVPYPDRPDSEAETEKQ"
     gene            7268..7600
                     /gene="cyaY"
                     /locus_tag="VGO02_06545"
     CDS             7268..7600
                     /gene="cyaY"
                     /locus_tag="VGO02_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEV7477378.1"
                     /translation="MLAPMNEAEFDSLAEAALRAIERSLEASGLDADFELKAGGVLEI
                     EFADGSKVIVNRHGAAREIWVAARSGGFHFRWDGAAWRDTRDGSELFAALSRVASLQS
                     GAPVKLAP"
     gene            complement(7597..9921)
                     /locus_tag="VGO02_06550"
     CDS             complement(7597..9921)
                     /locus_tag="VGO02_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEV7477379.1"
                     /translation="MAGAASLLAAGVLLVGLVIALAYPNLPSLQSLTEYQPKIPLRIY
                     TAEGVLIGEFGEERRAVVSISEVPPQLKNAVIAAEDERFYEHAGIDYLGVLRAAYANL
                     AAGGRSRQGASTITMQVARNFFLSSEKTLTRKLYEALLAFKIEHSLTKDQILELYINQ
                     IYLGQRAYGFGAASQIYYGKALDQLTLAETAMLAGLPKAPSTYNPIANPQRAKQRQQY
                     VLRRMTELGYITQEQHDEAYKAPLRTRREVREYPVHAEYVAEMVRQAIAETYPEDVYT
                     RGFRVYTTIRQADQETAYAALRRGVLEYDRNHGYRGAEGYAELPAAPDEDDFDEALAD
                     HPDNDTLIAAVVLSAAAGQVEAALRTGEKITLSGEGLKFGGRPLDPKAAPQRRIRRGA
                     IIRVQREAKNWQIVQLPEVEAGFISLDPQDGAIRALVGGFDFGRNKFNHITQAWRQPG
                     SSFKPFIYSASLEKGFTAATVIADEPVVLEAEQTGSQRWEPKNYDGKYEGPMRMRTAL
                     AKSKNMVSIRILDAIGPKYAQDYVTRFGFEPEKHPPYLTMALGAGSVTGWQMARAYSV
                     FANGGFLIQPYFILKVVDDRGNPLALANPLRAGEEGLRVIDPRNAFIMDNMMQDTMRI
                     GTAARAARLGRSDLAGKTGTTNEFVDAWFAGYQPNLVAISWVGFDQPKTLGKNQTGSV
                     VALPIWIGYMEKVLKDMPEAPRTAPDGVVTAQVQLLAPGGEGPSAPQPAEPKLVPEYF
                     YQESVPPPEVLRPLLDLPAPVVAPPPPPPTLPPA"
     gene            10136..11212
                     /locus_tag="VGO02_06555"
     CDS             10136..11212
                     /locus_tag="VGO02_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEV7477380.1"
                     /translation="MAIDLFKPKAPPLFGLDISSSSVKMLEIVEAGKGAYRVERYSIE
                     PLPRDAVVDGNINNLEAVAESVKRAHKRLATRTRHVAMAVPTGAVITKKIQVAASLTE
                     SELELQVESEANQYIPFSLEEVNLDFQSLGPAPTNPDEQEVMIAATRKEKVEDRVAVA
                     ESAGLKALVMDVESFAQQTALSLVVQSLPGGGKDQNVAVVDVGANVMQVTVLRNDQSI
                     YTREQAFGGNQLTQDIVSRYGMSPEEAENAKRSGGLPDDFEAEVLRPFMENLSMEVQR
                     ALQFFFTSTQYHAVDHILLAGGSAVIPGLEEVVNTRAQVPTTVGNPFAAMQTSPRIQL
                     KRLMVDAPSLIVACGLAMRRFDPT"
     gene            11209..11811
                     /locus_tag="VGO02_06560"
     CDS             11209..11811
                     /locus_tag="VGO02_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEV7477381.1"
                     /translation="MSARINLLPHREERRKRARQHFYVLAGGVGIIGILIVAAVHGFY
                     SSRIETQIERNRFLKAENTKLDKEIAEIQKLKDEIQALLARKQVIETLQTDRATTVYM
                     LEQLVRQMPEGVYLKSVVQKGTRINLLGYAQSNARVSTLMRNIEASPWLGSPGLVEVR
                     ASTVDRRRVSEFNMNLSLKRPAPEKDAPKGAPAKDAAKKG"
     gene            11815..12462
                     /locus_tag="VGO02_06565"
     CDS             11815..12462
                     /locus_tag="VGO02_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEV7477382.1"
                     /translation="MAINFNAIIEEFKRTNWQDPGTWHAAPKLLVLLAILVAIPVAGF
                     FADTQGQIEELEKGQGDEVKLKEEYLKKKQQAVNLDLHRQQLREIDTQFGALLRQLPN
                     RSQMDALLVDINQAGLGRGLQFELFRPAGSEITREFYAELPITVKVTGTYHDMGAFAS
                     DVGQLSRIVTLNDVSIQAGKDGILTMDATARTFRYLDDDEVSAQRKTASKKGGKR"
BASE COUNT         2132 a         4099 c         4248 g         2130 t
ORIGIN      
        1 gggcgggggt tctacccctg ctgcaacgcc gcctcgatct cccgcagctc cgcatcgggc
       61 gtgagcgcct tgcgcaagcg gcgcccgcag cgcgcgtacc agtactgcgc gttcggcagg
      121 tcgccttcct gccggtgcag cacggcgtgg atccagcagg cggtttcatc ctgctcgtcc
      181 tgctggacga tctcatgcgc ttcgcgccac ttgcccgaga gcgcgtgctc gacggcttta
      241 gccagatgcg ttttggagat cacgaaaaag tcttaccagg tcaggaagtt ggtagtttgc
      301 attcaggccg ctcggattgg gcagcaccca taccttggcg ccttcgaaga agtccggctg
      361 tatgccgatc gatgcgttgc gcaagccgaa tgcctggcag tacgcgccca ccccgaggaa
      421 ggcgaccgct ttcggccggt agcgcctgat tttcgccgcc aggcgcctgc ggccggcgac
      481 gaactcctcg ggcgcgagct cgtccgcggc cgccgtggcg cggttcacga ggttcaccac
      541 gccgaagcct gccttcagca attcgcgctg ctcggaagga tgcagcgggc gattcgtaaa
      601 cccggcttga tgcagggcag gccagaaacg attgccgggt cgcgcgaagt gatgaccggt
      661 ggcgcccgag tacaggccgg ggttgatgcc gcaaaacagt acccgcaagc cgggcgcgat
      721 cacatccggg atgctcttgc cgtttgcggc ggcaagctgc gccttgctag gtcggcctga
      781 cgatgtagcc gcccgatttc tcgtccgcgt caagcttgac caggcctttt tgctgcgctt
      841 ccttgaggag atcggcgaag gcgcgaaagc cgtagtaggt ctcgttgaag ccagggttgc
      901 ggcgcttgag cgcctgcttg accatcgagc cccagatctt ctcgtcggcg ccgcgctccg
      961 cgatgagcgc ctcgagcgtg ccgagcatca tgtcgaaggc ttcctgtttc ttgtcgccgc
     1021 cgttgccctt gtcgccgccc gtgctggccg gcgcggcctc cttgcgcttc ttcgccgtgc
     1081 gccgcttctg ctcgtcggcg cgcaccaggt cgtcgtagta gatgaactcg tcgcagttgt
     1141 tgatcagcag gtcggagctc gaattcttga cgccgacgcc gatcacggtc ttcgcgttct
     1201 cgcgcagctt ggagaccagc ggcgagaaat cggagtcgcc gctgacgatg acaaaggtgt
     1261 cgacgtgctc cttggtgtag cacaggtcga gggcgtcgac caccatgcgg atgtcggccg
     1321 agttcttgcc ggactggcgc acgtgcggga tctcgatcag ctcgaaggcc gaggcgtgca
     1381 tcgtcgcctt gaactccttg tagcggtccc agtcgcagta ggccttcttc accacgatcg
     1441 agcccttggc gagcagccgc tcgagcacct tctccatgtc gaacttgtcg tacttcgctt
     1501 cgcgcacgcc gagcgcgacg ttttcaaagt cgcagaacac ggcgaggtta cgcgcttcgg
     1561 ggttggccgg cacggggctc catctcgggt cgtcgcttgt ccttgttgag ccagtcctgc
     1621 aggcgctcga aatacaggta gatcaccggc gtaatgtaca gcgtaagcgc ctgcgacagc
     1681 agcaggccgc cgaccacggc gaggccgagc ggcatgcgcg cgccgccgcc ggcgccgaag
     1741 ccgatggcga tcggcaggct gcccatcagc gcggccatgg tggtcatcat gatcgggcgg
     1801 aagcgcacca ggccggcctg gtggatcgcc tgctcgggcg ccatgccgtc cttcctctgc
     1861 gcttcgatgg cgaagtcgat catcatgatc gcattcttct tcacgatgcc gaccagcatg
     1921 atgatgccga cgaaggcata catgttgagc tcgacgccga acgccatcag cgtgagcagc
     1981 gcgccaagcc ccgccgtcgg caggcccgac aggatggtga gcgggtggat gaagctctcg
     2041 tacaggatgc cgagcaccag atagatcaca aagatcgaca gcaggatcag gatgcccatt
     2101 ccctgcagcg agctctgaaa cgcctgcgcc gtgccggcga acgagccggt gagcgtggcc
     2161 gggacctgca tctcccgcgt cgcgcgctgg atctggtcga tcgcctccga cagcgccacg
     2221 cccgggggca ggtcgaacga gtaggtcacc gaggtgagct ggccgagatg cgttacctgc
     2281 aaaggcccga cgccgtaggc cagggaggcg accgcgttga gcggcaccag cgcgccggtg
     2341 ctggagcgca catagagcat ttgcagcacc gtcggatcgg tctggtagcg cggcgagagc
     2401 tccagaatca cccagaactg gttggtcgcg gtgtagatgg tcgatacctg ccgcgcgcca
     2461 taggcgttgt tgagcgccat ctcgatgctc tgcgccgaga cgccgagggc agaggccttc
     2521 tcgcgctcga tctgcaccgt cacctcgggg cgcgtgatct gcaggtcgct cgtcacgtcg
     2581 atcagcccgg gcagcgagcg cagcttctgc tcgatggtcg gtgcccagtg gtaaagctct
     2641 tcgatgctgg agctgcgcat cacgtactgg taggggctct tggtgagctg gccgccgatg
     2701 cggatcgacg ggatgttctg gatgaacacc ttggcgccga ggatgttggc gagcttcggg
     2761 cgcagctcgc gcacgatctg atcggcgggc ttgcgctctt taaacggctt caggttgacc
     2821 gtgacgcgcg cgatgttgag cagcggcgag ccggcgttgg cgccgacgaa cgccatggtc
     2881 gcctcgacgt tcggatcctt gcggatgatc tcgacgacct ggctctgcag ctcgatcatc
     2941 gcctcgtagg aggtgtcctg cgggccctcg acgaaacaca gcagctggcc ggcgtcctcg
     3001 ctcggcagga agcccttcgg cgcgcgcacg aacagcacgc ccgtgccgat gcagacgccg
     3061 agaaaggcga tcagcaccag gcgctggtgc cccagcgtcc agcgcagcga ggcatcgtag
     3121 gcgtcgcgcc aggcgtcgaa ggcgcgctcg aacgccatgt agagccggcc gtggcgctcg
     3181 tccttgctgt gcggcttcag gatgcggctg cacatcatcg gcgtcagcgt gagcgagacg
     3241 aagcccgaca gcagcactgc cgtgatgatc gtgacagcga attcgtgcag cagccggccg
     3301 aggatgccgc ccatgaacag caccggaatg aacaccgcgg cgagcgagat ggtcatcgag
     3361 atgatggtga aaacgatttc cctcgagccg tcgagcgtgg cctgcaacac gctcttgccc
     3421 tgctcgagat ggcgcgtgat gttctccagc atcacgatgg cgtcgtcgac cacgaagccg
     3481 acgcagaggg ttagtgccat caatgagatg ttgtccaggc tgtagccgaa cacatacatc
     3541 accgcgaagg tgccgatgat gcaaagcggc agcgcaatcg cggggatcac ggttgcggcg
     3601 acgttgcgca ggaacagaaa gatcaccatc accaccaggc agagcgctag gtacagggtg
     3661 aactgcacct cctggaccga ggcgcggata gtctgcgagc ggtcgtagag gatgtcgatg
     3721 ttgatcgcag gcggcacctc ggcgcggaag gtcggcagca gcttcttgat gttatcgacc
     3781 acctcgatgg tgttggtgcc gggctggcgc tggatggcga gcacgatgcc acgcttgccc
     3841 ttgaaccagg cgccgagctt gtcgttctgc acgctgtccg tgacggtgcc cagctcctgc
     3901 cggcggatcg gcgagccgtt gcggtaggtg acgatgatcg gccggaaggc ggcggcgtcg
     3961 ttcagctggc cggtcgcctg caccgcgaag acgcggtcct tgccgtagag ggtgccggtg
     4021 ggcaggttga cgttcgcctg cgaaaccgcc tgctccacct cgttgatgcc gacgccgcgc
     4081 gccgccagcg cgttggggtc gacctggatg cgcaccgcgt atttctgctg cccgaatacc
     4141 tgcacctggg cgacgccgtt gatcgtcgag atgcgctgcg cgaggttggt ctcggcatac
     4201 tcgtttacca ccgcgatcgg cagcgcgtcc gagctcagcg caaggaagaa caccgggaaa
     4261 tcggccggat tgaccttgcg gaaagacggc ggcgccgaca tcgtcgccgg cagctggcgc
     4321 gaggcggagg cgatggcgga gttcacgtcc tgcgcggccg catcgatgtc gcggtcgagg
     4381 ctgaactgga tggtgatgcg cgtcacgccc tggccgctca ccgaggtcat ctcgtcgatg
     4441 ccggcgatgg tcgagaactg ccgctcgagc accgtggcga ccgcggaagc catggtctcc
     4501 gggctggcgc cgggcagctc ggcggtgacc tggatggtgg ggaagtcgac gttgggcagc
     4561 gtcgagaccg gcagcaggcg atacgccgca atgccgaaaa tcagcaggcc ggccatcagc
     4621 agcacggtca tcaccggccg ccgcacgaac ggctcgcaga agttcacgac gcttccgccg
     4681 ctggcttggc gatctgtacc ggggtcttcg gcccgaggcg cagctgtccg cgcgtcacca
     4741 cccgctcgcc cttggcgagc ccggtcgcga tgatggcgcg ctcgccatcg gcgcgctgca
     4801 ccgtgacctt gcgcagctcg gcgagcaggt cgggcccgac gacgtagacg tactggccct
     4861 ccggaccggt ctgtaccgcc gttgccggaa tgatgatggc atcggcctgc tcataaaggc
     4921 gcaggctgac attgacgaac tgtcccggcc agagcgccgc ctcctcgttg gggaactgcg
     4981 cgcgcagctt gatcgtgcca gtggtcggat cgactgcgtt gtcgacgaag atcaactgcc
     5041 cggggatgcg cgccacagcc ggatcagacg gcaccacctc gacgctgagc gggccgcccg
     5101 ccatgtactt gcgcacttcc ggcaggttct gctcggggac cgagaagttg acgtagatcg
     5161 gcttcacctg gttgatgacg accagggcgc cgacgtcgtt cgccttcacc aggttgcccg
     5221 cctgcagcag gaccttgccg acgtagccgt ccagcggcga ggtgatggtg cagtactcga
     5281 ggttcaggcg cgcgttctcg accgccgcct ggctcgacct ggcgaccgct tcggcggtct
     5341 cggcgttggt gcggatctgc gcgaacgcct ctttcgagat gaaattcttg tcgagcagct
     5401 cctggtagcg cgtcgcctgc gagcgcgcct ggtcgcgcgc cgccttgtcg cgcatcgcgt
     5461 tcgcctcggc ctggcgcaac gtcgattggt acgggcgcgg gtcgatgcgg aacagcgcca
     5521 cgcccttttt caccggatcg ccctcgcgga aattcacttc cacgatctgt ccgtcgacgc
     5581 gcgccttgat cgacaccgtc tggtaagcct cgacgttgcc gatccccgtg aggcgcaccg
     5641 gaaccgtctg ctgctgcgct gcggcaacgt tcaccggcac cgccggcggg cccttgcccg
     5701 cgcgcttgcc ttccttcgcc cggccgtcgg aaacgaagta ggtgcccaga ccggccgcgg
     5761 cgaggaccgc gaccaggaca gcagcaaata gcaaacgttt gctcataaac cgaccattgt
     5821 cagcccggaa tgcgctccag ggcaaacagc tgctcgacgc tttcccggct gcgtaccaga
     5881 tgggctgctg ccccgtcgac gagcacttcc gcggcccgcg gccgcgagtt gtagttcgag
     5941 ctcatgacca tcccgtaggc gccggccgac atgaggacca gcaggtcccc ggcgcgtgca
     6001 gccaggcgcc gctccttggc gagaaagtcg ccgctttcgc agaccgggcc gacgatgtcg
     6061 taaacagtct gcgctgcgcc ctcttccggc tggcgcacgg cgcgcacctc gtgccaggcc
     6121 tcgtacagcg ccgggcgcac gaggtcattc atcgccgcgt cgaccaccac gaaattgcgc
     6181 gcagcgccag gcttgacgta ctcgacccgt gaaagcagca ccccggcgtt acccacgata
     6241 gagcggcccg ggtcgacgat cagcgtttcg cggcggccgg caagcatgcc gagtgcgcca
     6301 tcgaggaacc cggcgatcgg ctccggagtc tcgtcgcggt agcggatgcc gatgccgccg
     6361 ccgatgtcga tgtgcttgag ggcgattccg gcgcgcccga ggcgatcggc gagcgcggcg
     6421 atgcgcccgg cggcttcgac gaacggcgcg gcgcgggtga gctgcgagcc gatgtggcag
     6481 ccgatgccga cgagctcgat gtgcggcagg cgcgccgcgg ctgcgtagat tctctcggcc
     6541 tcggcatacg cgatgccgaa cttggtttcg cgcaggccgg tcgagatgta cggatgcgtg
     6601 cgcgcatcga tgtcgggatt gacgcgaaac gccaccggtg cgcgccggcc gagacgccct
     6661 gccacctcgg acacgcgcgc gagctcgctt tccgactcca gattgaggca caggatgttc
     6721 ttctccagcg cgagctggat ctcggcctgc gtcttgccca cgccggagaa caaggtcttg
     6781 cgcgcatcgc cccccgcggc gaggacgcgg gcgagctcgc ctcccgaaac gatgtcgaag
     6841 ccggcgccca gccgcgcgag cagcgcgagc accgcgagat tggagttcgc cttcaccgag
     6901 tagcagacga gcgaatcgcg cccctgcagc gcgcgcgcat actcctggta cgcaccctcg
     6961 atggcggcgc gcgaatagac atagcagggc gtgccgaagc gccgggcgat atcgtccagc
     7021 ggtacctgct cggcgcacag cacgccgttg cggtaggcga aggtcatgct tactgcttct
     7081 cggtttcggc ttcggagtcg ggccggtcgg gatacggcac cggcttcacc ggcgccggct
     7141 tgggcggctt cattccgggg ggcggcttgt cacgcagata gagcggtccc ttctgcccgc
     7201 atccggcgcc gagcgcaaga accatcccgg aggcgatgaa aagcggaagg aaacgcatag
     7261 aatccgcatg ttagcaccca tgaacgaagc tgaattcgat tcccttgccg aggcagcgct
     7321 gcgcgccatc gagcgctcgc tcgaggcgag cggcctcgat gcggacttcg agctgaaagc
     7381 gggcggcgtg ctggagatcg agttcgccga cggttccaag gtgatcgtca accggcacgg
     7441 cgcggcgcgc gagatctggg tcgcggcgcg ctcaggcggc tttcacttcc gatgggacgg
     7501 cgcagcctgg cgcgatacgc gcgacggctc cgagctgttc gcggcgcttt cccgcgtcgc
     7561 ctcgctgcaa tccggagcgc ccgtgaagct agcgccctag gccgggggca gggttggcgg
     7621 aggcggcggc ggtgcaacga ccggcgccgg cagatcgagc agcgggcgca gcacctcagg
     7681 cggcggcact gactcctggt agaaatactc cggcaccagc ttgggctccg cgggttgcgg
     7741 cgcggacggc ccctccccgc cgggcgcgag tagctgcacc tgggccgtaa cgacgccgtc
     7801 cggcgcagtg cgtggcgcct cgggcatgtc cttgagcacc ttctccatat agccgatcca
     7861 gatcggcagg gccaccacgc tgcccgtctg gttcttgccc agtgtcttcg gctggtcgaa
     7921 gccaacccac gaaatggcga cgagattcgg ctggtagccg gcgaaccacg catccacgaa
     7981 ttcattggtc gtacccgtct tgcccgcaag gtcgctgcgg ccaagccgcg cagcgcgcgc
     8041 cgcggttcca atgcgcatcg tgtcctgcat catgttgtcc atgatgaagg cgttgcgcgg
     8101 gtcgatcacg cgcaggcctt cttcgccggc gcgcagcgga ttggcgaggg ccagcgggtt
     8161 gccgcggtcg tccacgacct tgaggatgaa gtagggctgg atcaggaagc cgccgttggc
     8221 gaacaccgag taggcgcgcg ccatctgcca gccggtcacc gagcccgcac cgagcgccat
     8281 ggtgaggtac ggcgggtgct tttccggctc gaagccgaag cgcgtcacgt agtcctgcgc
     8341 gtacttgggc ccgatcgcgt ccaggatgcg gatcgacacc atgttcttcg acttggcgag
     8401 cgcggtgcgc atgcgcatcg gcccctcgta cttgccgtcg tagttcttcg gctcccagcg
     8461 ctggctgccg gtctgctccg cctccaggac caccggctcg tcggcaatca cggttgccgc
     8521 ggtaaagccc ttttccagcg acgccgagta gatgaaaggc ttgaacgagg agccgggctg
     8581 gcgccaggcc tgcgtgatgt ggttgaactt gttgcggccg aagtcgaaac cgccgaccag
     8641 cgcgcggatc gcgccgtcct gcgggtcgag cgagatgaaa ccggcttcga cctcgggcag
     8701 ctgcacgatc tgccagttct ttgcctcgcg ctgcacgcgg atgatcgcgc cgcggcggat
     8761 gcgccgctgt ggcgccgcct tcgggtcgag cgggcggccg ccgaacttca gcccctcgcc
     8821 gctcagggtg attttctcgc ccgtgcgcag tgcggcttcg acctggcccg cggccgcgct
     8881 cagcaccact gcggcgatca gcgtgtcgtt gtcgggatgg tcggcgagcg cttcgtcgaa
     8941 atcgtcctcg tcgggggcgg ccggcagctc cgcgtaaccc tcggcgccgc ggtagccatg
     9001 gttgcggtcg tactccagca cgccgcggcg caacgcggca taagcggtct cctggtcggc
     9061 ctgccggatc gtcgtataga cgcggaagcc gcgcgtgtag acgtcctccg gataggtctc
     9121 ggcgatcgcc tggcggacca tttcggccac gtattcggcg tgcacggggt actcgcgtac
     9181 ttcgcgccgc gtgcgcagcg gcgccttgta cgcctcgtca tgctgttcct gcgtgatgta
     9241 gcccaattcc gtcatgcggc gcaggacgta ctgctggcgc tgcttggcgc gttgcgggtt
     9301 ggcgatcggg ttgtaggtcg acggcgcctt tggcagcccg gcgagcatcg ccgtctcggc
     9361 cagggtgagc tggtcgagcg ccttgccgta gtagatctgc gaggccgcgc cgaagccgta
     9421 cgcgcgctgg ccgagataga tctggttgat gtacaactcg aggatctggt ccttggtcag
     9481 gctgtgctcg atcttgaagg cgagcagggc ttcgtagagc ttgcgcgtga gcgtcttctc
     9541 cgaggacagg aagaagttgc gtgccacctg catggtgatg gtgctggcgc cctgccggct
     9601 gcggcctccc gccgccaggt tggcgtaggc ggcgcgcaac acgcccagat aatcgatccc
     9661 ggcatgctcg tagaaccgct cgtcctcggc ggcgatgacc gcgttcttga gctggggggg
     9721 aacctcgctg atcgagacca ccgcccggcg ctcctcaccg aactcgccga tcagcacccc
     9781 ttcggctgtg taaatgcgca gcggaatctt cggctggtac tctgtaagtg actgaaggga
     9841 cgggagattc gggtaagcga gggcaatcac caagccgacc aggagaacgc cggcggcgag
     9901 caggctcgcg gcgcctgcca aggggtatag aacgtagcgg ggtaacttca cgaagggctt
     9961 agaccgtcgc cggggccaat ttgcacgagc acaggggcga gagcatcata cccgcccttc
    10021 cggcgtggaa aattgactag acacccgggg gggttgttgc tagcatctaa ggcgatttct
    10081 acgtatcgcg cctaaaggta cgatacataa agggaaatcg taagggacga aaataatggc
    10141 aatcgacttg ttcaagccga aggcgccacc gttgttcggg ctggacatca gttcgtcctc
    10201 ggtgaagatg ctggagatcg tagaagccgg caagggcgcc taccgcgtgg agcgctactc
    10261 gatcgagcct ctgccgcgcg acgccgtggt cgacggcaac atcaacaacc tcgaggccgt
    10321 ggccgagtcg gtcaagcggg cgcacaagcg ccttgccacc cgtacccgcc acgtcgccat
    10381 ggccgtcccg accggggcgg tgatcaccaa gaagatccag gtcgccgcca gtctgaccga
    10441 atccgagctc gagttgcagg tcgagagcga ggccaaccag tacatcccct tctcgctcga
    10501 ggaagtgaac ctcgacttcc agtcgctcgg cccggcgccg accaaccccg acgagcagga
    10561 ggtcatgatc gcggcgacgc gcaaggagaa ggtcgaagac cgcgtcgcgg tcgccgagtc
    10621 ggcagggttg aaggcgctgg tgatggacgt cgagtcgttc gcccagcaga ccgccctttc
    10681 gctcgtggtg cagagcctgc cgggcggcgg caaggaccag aacgtcgcgg tggtggacgt
    10741 cggcgccaac gtgatgcagg tgacagtgct gcgcaacgac cagtcgatct acacgcgcga
    10801 gcaggcgttc ggcggcaacc agctgacgca ggacatcgtc agccgctacg gcatgagccc
    10861 ggaggaagcc gagaacgcca agcgctccgg cggcctgccc gacgacttcg aggccgaagt
    10921 gctgcgcccg ttcatggaga acctgtcgat ggaagtgcag cgcgcgctgc agttcttctt
    10981 cacctcgacg cagtaccacg cggtcgatca catccttctc gcgggcggct cggccgtcat
    11041 tcccgggctc gaggaggtcg tgaacacgcg cgcccaggtg ccgaccaccg tcggcaaccc
    11101 gttcgccgcg atgcagactt cgccgcgcat ccagctcaag cgcctgatgg tcgacgcgcc
    11161 ttccctcatc gtggcctgcg gcctcgccat gcgcaggttc gatccgacat gagcgcgcgc
    11221 atcaacctac tgccgcaccg cgaggagcgg cgcaagcgtg cccgccagca cttctacgtg
    11281 ctcgccggcg gcgtcggcat catcggcatt ctgatcgtcg ccgcagtgca cggcttctat
    11341 tcctcgcgca tcgagacgca gatcgagcgc aaccgcttcc tgaaggccga gaacaccaag
    11401 ctggacaagg agatcgccga gatccagaag ctgaaagacg agatccaggc gctgctcgcg
    11461 cgcaagcagg tgatcgagac gctgcagacc gaccgcgcca ccaccgtcta catgctcgag
    11521 cagctggtgc gccagatgcc cgagggcgtc tatctgaaat cggtggtgca gaaaggcacc
    11581 cgcatcaacc tgctgggcta cgcgcaatcc aacgcgcgag tctcgaccct gatgcgcaac
    11641 atcgaggcct ccccctggct cggcagcccc ggcctggtcg aagtgcgggc aagcaccgtc
    11701 gaccggcggc gggtctcgga attcaacatg aatctgagcc tgaagcgccc cgccccggag
    11761 aaggatgcgc cgaaaggcgc gccggcgaag gacgcggcga agaaaggctg agccatggcg
    11821 atcaatttca acgcgatcat cgaggagttc aagcgcacca actggcagga tcccggcacc
    11881 tggcacgcgg cgccgaagct gctggtcctg ctcgcgatcc tggtggcgat tccggtggcc
    11941 gggttcttcg ccgacacgca gggccagatc gaggagctcg agaagggcca gggcgacgag
    12001 gtcaagctta aagaggaata cctgaagaag aagcagcagg cggtcaacct cgacctgcac
    12061 cgccagcagc tgcgtgagat cgacacgcag ttcggcgcgc tgctcaggca gctgccgaac
    12121 cgctcgcaga tggatgcgct gctggtcgac atcaaccagg ccggcctggg ccgcggcctg
    12181 cagttcgagc tgttccggcc ggccggctcc gagatcacgc gcgagttcta cgccgagctg
    12241 ccgatcacgg tgaaggtgac cggcacctat cacgacatgg gcgccttcgc gagcgacgtc
    12301 ggccagcttt cccgcatcgt gacgctgaac gacgtttcca tccaggcggg caaggacggc
    12361 atcctgacca tggacgccac cgcgcgcacc ttccgctacc tcgacgacga cgaagtctcg
    12421 gcgcagcgca agacagcctc caagaagggc ggcaagcgat gaggtcggcg gcgctgcatg
    12481 cggcgtggct cgccgcggcc ctcctgctcg tcgcctgcac cggcgaggag ttcggcgacc
    12541 tgaaggccga gctcaacgag aagagcaagg acttccgcgg ccgcgtcgag cccttgccgc
    12601 aggtgaagc
//
LOCUS       DASYJA010000300         1810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2494937, whole genome shotgun sequence.
ACCESSION   DASYJA010000300 DASYJA010000000
VERSION     DASYJA010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2494937"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /gene="cca"
                     /locus_tag="VGO02_06570"
     CDS             <1..918
                     /gene="cca"
                     /locus_tag="VGO02_06570"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561844.1"
                     /note="catalyzes the addition and repair of the essential
                     3'-terminal CCA sequence in tRNAs without using a nucleic
                     acid template; phosphohydrolase activities include
                     hydrolysis of pyrophosphate, 5'-nucleoside tri- and
                     diphosphates, NADP, and 2'-AMP with the production of Pi,
                     metal-dependent phosphodiesterase activity for 2',3'-cAMP,
                     2',3'-cGMP, and 2',3'-cCMP, and hydrolysis 2',3'-cyclic
                     substrates with the formation of 2'-nucleotides and
                     3'-nucleotides; these phosphohydrolase activities are
                     probably involved in the repair of the tRNA 3'-CCA
                     terminus degraded by intracellular RNases; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA tRNA nucleotidyl
                     transferase/2'3'-cyclic
                     phosphodiesterase/2'nucleotidase/phosphatase"
                     /protein_id="HEV7477383.1"
                     /translation="PVGKDFPVFLHPQTHEEYALARTERKSGRGYKGFTVYAAPDVTL
                     EDDLRRRDLTINAMAKAEDGTLIDPFSGKKDLEKKVLRHVSDAFSEDPVRILRVARFA
                     ARFGFGIAQETMDLMKNMVASGETDYLVPERVWQEFAKGLVEEHPERMFEVLEACGLR
                     ERLLPELETQPEVLSGSLAVRFALLCWSLAEDEVETLCRRLRVPNDVRDLALLVSRNR
                     NSLENARRPEELLELLKKTDALRRPERFAELLEVARLAAHGVDLARLERARAAAAAVD
                     AGAIAAKASAADIPRLIDEARLQAIAAAK"
     gene            complement(911..1105)
                     /locus_tag="VGO02_06575"
     CDS             complement(911..1105)
                     /locus_tag="VGO02_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEV7477384.1"
                     /translation="MMKWLITVVVVLVVFSLVAPRMGRFGLGRLPGDFQVPARGRIYY
                     IPIASTLVLSLAVWLLGKII"
     gene            1104..1721
                     /locus_tag="VGO02_06580"
     CDS             1104..1721
                     /locus_tag="VGO02_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEV7477385.1"
                     /translation="MIDFYTWSTPNGRKVSIMLEECALPYRVHKINIGTNAEQFAPEY
                     LKINPNGKIPSIVDPDGPGGKPIAMMESGAILIYLAEKTGKFFSQKYKYEVLQWLMFQ
                     MGGVGPMFGQAHHFMRAKKDEVPYGSERYGTEAKRLYGVMDRHLAKSEFFAAEYSIAD
                     MAIYPWVARHEWHRVDLAAFPQVKRWYEELGARPAVAKGMTIPQV"
BASE COUNT          350 a          567 c          596 g          297 t
ORIGIN      
        1 ccggtcggca aggactttcc ggtgttcctg catccgcaga cgcacgagga atacgccctc
       61 gcgcgaaccg agcgcaagag cgggcgcggc tacaagggct tcaccgtgta cgccgccccg
      121 gacgtcacgc tcgaggacga cctgcgccgg cgcgacctca ccatcaacgc gatggcaaag
      181 gccgaagacg gcacgctgat cgatcctttt tccggaaaaa aagatctgga gaaaaaggtg
      241 ctgcgccatg tcagcgacgc attctccgaa gatcctgttc gcatcctgcg cgtcgcgcgc
      301 ttcgcggcgc gcttcggttt cgggattgcg caggaaacga tggatttgat gaaaaacatg
      361 gtcgcgtcgg gtgagacgga ctatctggtg cccgagcgcg tctggcagga attcgccaaa
      421 ggcctcgtgg aagagcatcc cgagcgcatg ttcgaggtgc tcgaggcctg cgggctgcgc
      481 gagcggctgc tgccggagct cgagacgcag cccgaggtgc tgtcgggctc gcttgcggtg
      541 cgcttcgcgc tgctttgctg gtcgctcgcc gaggacgagg tggagaccct ctgccggcgg
      601 ctgcgcgtgc cgaacgacgt gcgcgacctt gcactgcttg tctctcgaaa ccgtaacagt
      661 ctcgagaacg caaggcgtcc ggaggagcta ctcgaattgc tgaaaaaaac cgacgccctg
      721 cgccgccccg agcgcttcgc cgagctgctc gaggtggcgc ggcttgcggc gcatggcgtc
      781 gatctggcgc gcctggagcg cgcgcgcgcg gccgcggcgg cggtcgacgc cggcgcgatc
      841 gccgccaaag cctccgctgc cgacattccg cggctgatcg acgaggcccg cctgcaggcg
      901 atcgcggcgg ctaaatgatc ttgccgagca gccagacggc gagcgaaagc accagcgtgc
      961 tggcaatcgg gatgtagtag attcgaccgc gcgccgggac ctggaagtcg ccgggcaggc
     1021 ggccgaggcc gaagcggccc atgcgcggcg cgaccagcga gaaaaccacc aatacaacca
     1081 ctacggtaat cagccatttc atcatgatcg atttctatac ctggagcacg ccgaacggcc
     1141 gcaaggtctc cattatgctg gaggagtgcg cgcttccgta tcgcgtgcac aagatcaaca
     1201 tcggcaccaa cgcggagcag ttcgcgcccg agtacctgaa gatcaacccg aacggcaaga
     1261 tcccgtcgat cgtcgatccg gacggtcccg gcggcaagcc gatcgcgatg atggagtcgg
     1321 gcgcgatcct catctatctc gcggagaaga ccggaaaatt tttctctcaa aaatacaaat
     1381 atgaagttct ccagtggctg atgttccaga tgggcggcgt cggtccgatg ttcggccagg
     1441 cgcaccactt catgcgcgcg aagaaggacg aggtccccta cggcagcgag cgctacggca
     1501 cggaggccaa gcggttgtat ggggtgatgg accgccacct ggccaaaagc gagttcttcg
     1561 ccgccgagta ctcgatcgcg gacatggcca tttacccctg ggtggcgcgc cacgaatggc
     1621 accgcgtcga cctggcagcc ttcccgcagg tgaagcgctg gtatgaggaa ctcggcgcac
     1681 gcccggccgt ggccaaggga atgaccatcc cgcaggtgta gtcgtggcgc tttgccaacg
     1741 gcccctgtcg cctgccggcg acagagtacg ggttatccac cgattcgcgt ttgcatggcg
     1801 aaaccggggg
//
LOCUS       DASYJA010000301         6099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2950363, whole genome shotgun sequence.
ACCESSION   DASYJA010000301 DASYJA010000000
VERSION     DASYJA010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2950363"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VGO02_06585"
     CDS             complement(<1..395)
                     /locus_tag="VGO02_06585"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEV7477386.1"
                     /translation="MLEPGQAAPVFDLPDADMQNVSLSEFKGKKHVVLYFYPRDGAPS
                     CTMVTTDFSDHEEQFVKHDCVVLGVSRDDCLTHADFRDRNGVTIGLLSDPEGEVCRKY
                     GVIQEKESEGVRREHLVRSTFLIDKKGVIR"
     gene            480..1502
                     /locus_tag="VGO02_06590"
     CDS             480..1502
                     /locus_tag="VGO02_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV7477387.1"
                     /translation="MKLLGGLAPRTFLRRHWQKRPLLVRAALPGFTGVLDARALAALA
                     RRDDVESRIVKRRGARWETAHGPFSREITLRKNSTLLVSGVNLHLPAADALLRRFAFV
                     PQARLDDLMVSYATPGGGVGPHVDSYDVFLLQGPGRRRWKIWLSNKNMKEYVVSPGDL
                     LYLPPGLKHDGVALEACYTYSIGFRAPRGAELGAAFLDWLHERGLPDARYRDPGLRPA
                     RRAGRIPAEMLVFVKNFLRRIKWTDQDASTFLGEFLTMPKPHVVFGPSRSRGPLARLD
                     PKTQLLYSGNRFFINGESLSVDARKAKSLRNLADRRSAPATQPGALAGLIAEWHRAGY
                     LHLEKP"
     gene            1499..1984
                     /locus_tag="VGO02_06595"
     CDS             1499..1984
                     /locus_tag="VGO02_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477388.1"
                     /translation="MSETSYERFDDRAGFQAAVDRLLEQPGRELRAFDPDGTALMLND
                     PGRIERIERFLLASRTRRLYLVLHDPEHVTRHCPRMMSLLARFAHAIQVNRTHDEIRE
                     LQDAFLVLDSLHYVRRPVAQFFRGAIGLNDDNEGQAMRGRFGEIWSASYPAVSATTAG
                     L"
     gene            complement(1988..2758)
                     /locus_tag="VGO02_06600"
     CDS             complement(1988..2758)
                     /locus_tag="VGO02_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7477389.1"
                     /translation="MRFASLASGSGGNCLVAEAGGTRLLIDCGLNLRDTEKRLARLGL
                     APAELAGILITHEHGDHAGCAFDFAAAHRVTLYLTYGTLVALEAEGKVLEGVKKVILT
                     AREDIAIGELEVAPFTVPHDAREPVQFVLSDGAVRLGVLTDIGVSTPHVERTLSGLDA
                     LVLECNYDCDMLWAGPYPKWLKERIAGPFGHLDNRESERLLGALDRSRLKHVIGAHLS
                     QHNNTPALARACLARALGCEENWVGLATQDDGFDWRSV"
     gene            complement(2761..3891)
                     /gene="bamC"
                     /locus_tag="VGO02_06605"
     CDS             complement(2761..3891)
                     /gene="bamC"
                     /locus_tag="VGO02_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEV7477390.1"
                     /translation="MAVAATTLCLVACASIDDMLDSKVDYKSQAKGKTMPTLEVPPDL
                     TSPARDNRYVVPETGRTSATLSGYQAERKEQSRAGNTQVLPQVEQMRVERAGSERWLV
                     VANETPERLWPMVKDFWQENGFLIKTELPEAGVMETDWAENRAKVPGGVLRDTFTKFL
                     DQVYSTSERDKFRTRLERTADGKGSEVYISHRGMNEVYTSPRGVAGENAQTVWSPREP
                     DPGLEAEFLRRLMVRLGYQAERAKDLVAASPQSQRAEIRKGIDGAELLQVNEPFDRAW
                     RRVGLVLDRVGFTVEDRDRQKGLYFVRYADPESEMAIKDRDRGFFGKLFGGESKLKAE
                     QYRVQVRQADENSQVQVLNKDGATENTKTTQRILTLLHEQLK"
     gene            complement(3900..4808)
                     /gene="dapA"
                     /locus_tag="VGO02_06610"
     CDS             complement(3900..4808)
                     /gene="dapA"
                     /locus_tag="VGO02_06610"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEV7477391.1"
                     /translation="MITGSLVAIVTPMRADGALDLAAFRKLIDWHVAEGTDGIVVVGT
                     TGESPTVDFDEHKELIRIAVQHSKGRIPIIAGTGGNSTAEAIELTESAKKAGATASLS
                     VVPYYNQPTQEGMYQHFRKIAETVDLPMILYNVPARTVADMQNDTVLRLTEVPGIIGI
                     KDASANIERGTDLIRRMPRNFAIYSGEDVTALALILLGGHGVISVTANVAPKLMHQMC
                     GAALVGDVKKAREINLKLLMLHQRLFIETSPAPVKWALAEMSMIENGLRLPLVPMSDK
                     FQGAVREALHEAGIALPGLRVVEGRA"
     gene            complement(4805..5320)
                     /locus_tag="VGO02_06615"
     CDS             complement(4805..5320)
                     /locus_tag="VGO02_06615"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7477392.1"
                     /translation="MEDPSEWVLRWAPLVERGPVLDVACGGGRHSRLFSERGMQVVAV
                     DRENQVLSKEILFVKADLENGNPWPFAGQRFAAIVVTNYLHRPLFAQLAAALDEGGVL
                     IYETFMVGNEKFGRPSNPEFLLRPGELLEAFAGLEVAAFEQGRVERPKAAMIQRLCAL
                     RGRAERIRIAP"
     gene            5372..>6099
                     /locus_tag="VGO02_06620"
     CDS             5372..>6099
                     /locus_tag="VGO02_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7477393.1"
                     /translation="MDNRPRVLIVDDSKVVRTTLAKVIRSTFDVREEADGEAGWAAID
                     GDPAIVAVISDLGMPKLDGMGLLARIRNSPQPRVRDLPFLVISGNEDDDTRDRARAAG
                     ANDFISKSTKGAEAIARIDNLLRLVKAKQDLEASADALKVSDDDKMWDQPTGAFTSSY
                     LLTEGGKHYSHAKRHGTGLSAVSFRIDNYSDVEGKVGKVPAEQLLARIAKLVQETLRA
                     EDSVGRMGPALFSAILSSTAAEQAV"
BASE COUNT          974 a         2070 c         2008 g         1047 t
ORIGIN      
        1 cggatgacgc ccttcttgtc gatgaggaag gtcgagcgca ccaggtgctc gcggcgcacg
       61 ccctcgctct ccttctcctg gatcacgccg tacttgcggc acacctcgcc ctccgggtcg
      121 gagagcaggc cgatggtgac gccgttgcgg tcgcgaaagt cggcgtgcgt caggcagtca
      181 tcgcgcgaca cgccgagcac cacgcagtca tgcttgacga actgctcctc gtggtcggag
      241 aagtcggtgg taaccatcgt gcagctgggc gcgccgtcgc gcggatagaa gtaaagcacg
      301 acgtgcttct tccccttgaa ctccgacagg ctgacgttct gcatgtcggc atcgggaaga
      361 tcgaaaactg gcgcggcttg tcccggttcg agcatctgcg gcgattctat tccaaaacat
      421 ttttactttg acaagtaccg tgcgcagctt gtagcgggcg cgctacatat aatgcctcga
      481 tgaaattact cggcggcctc gcaccgcgca cgttcctgcg ccgtcattgg cagaagcgcc
      541 cgcttctcgt gcgagccgcg ctgccaggct tcacgggagt gctcgatgcg cgggcgctgg
      601 cagcgctcgc caggcgcgac gacgtcgaat cccgcatcgt gaagcggcgc ggcgcgcggt
      661 gggagaccgc gcacggcccg ttcagcagag aaatcacttt aagaaagaac tcgacgctgc
      721 tggtaagcgg cgtaaacctt catctgcctg cggccgacgc gctgctgcgc cgtttcgcat
      781 tcgtgccgca ggcgcggctc gacgacctga tggtgagcta cgcgacgccg ggcggcggcg
      841 tcggcccgca cgtcgactcc tacgacgtgt tcctgctgca gggcccgggc aggcgccgct
      901 ggaaaatctg gctttcgaat aagaacatga aggaatacgt ggtcagcccc ggcgatctgc
      961 tctacctgcc gccgggcctg aagcacgacg gcgtcgcgct cgaggcctgc tatacctatt
     1021 cgatcggctt tcgcgcgccg cgcggcgcgg agctcggcgc cgcgttcctc gactggctgc
     1081 acgagcgcgg cctacccgac gcccgctacc gcgatcccgg cctgcggccc gcgcgccgcg
     1141 ccggccggat cccggccgag atgctcgttt tcgtgaaaaa tttcctgcgc aggatcaagt
     1201 ggactgatca ggacgcgtcg acctttctcg gcgaattcct gaccatgccg aaacctcacg
     1261 tcgttttcgg gcccagccgc agccggggcc cgctcgcgcg cctcgatccc aaaacgcagc
     1321 tcctctactc gggcaatcgc ttcttcatca atggagaatc cctgtcggtg gatgcacgaa
     1381 aagcaaaatc cctgcgaaat ctcgcggaca ggcgtagcgc cccggcaacg caaccagggg
     1441 cgcttgcagg tctgatcgcg gaatggcatc gtgcaggcta cttgcacctc gaaaagccat
     1501 gagcgaaacc tcatacgagc gcttcgacga ccgcgccggc ttccaggcgg cggtcgaccg
     1561 cctgctcgag cagccgggcc gcgagctgcg cgccttcgac cccgacggca cggccctcat
     1621 gctgaacgac ccggggcgca tcgagcgcat cgagagattc ctgctggcga gccgcacgcg
     1681 gcgcctgtac ctggtgctgc acgaccccga gcatgtgacg cgccactgcc cgcgcatgat
     1741 gtcgctgctc gcgcgcttcg cgcatgccat ccaggtcaac cgcacgcacg acgagatccg
     1801 cgagctgcag gacgccttcc tggtcctcga ctccctgcac tacgtgcgcc ggccggtggc
     1861 gcagttcttc cgcggcgcga tcggcctgaa cgacgacaac gagggccagg cgatgcgcgg
     1921 caggttcggc gagatctggt cggcctccta cccggccgtc tccgccacca ccgccgggct
     1981 gtaattccta gacgcttctc cagtcgaagc cgtcgtcctg cgtggcgagg ccgacccagt
     2041 tctcctcgca gccgagcgcc cgcgccaggc aggcgcgcgc cagcgcgggc gtgttgttgt
     2101 gctgcgagag atgggcgcca atgacgtgct tcagcctgga gcggtcgagc gcgccgagca
     2161 ggcgctcgga ctcgcggttg tcgaggtggc cgaaggggcc ggcgatgcgc tccttgagcc
     2221 acttcggata ggggccggcc cagagcatgt cgcaatcgta gttgcattcc aggaccagcg
     2281 cgtccaggcc ggagagcgtg cgctcgacgt gcggcgtcga cacgccgatg tcggtgagca
     2341 cgcccaggcg caccgcgccg tcggacagga cgaactgcac cggctcgcgc gcatcgtgcg
     2401 gcacggtgaa gggagccacc tcgagctcgc cgatggcgat gtcttccctg gcggtaagaa
     2461 tcactttctt gacgccttcg agcaccttgc cctccgcttc cagggccacc agggtgccgt
     2521 aggtgagata gagcgtgacg cgatgagccg cggcgaagtc gaaggcgcag ccggcgtggt
     2581 cgccgtgctc gtgggtgatc aggatgccgg cgagctcggc cggcgcgagc ccgaggcgcg
     2641 ccagccgctt ctcggtgtcg cgcaggttga ggccgcagtc gatcagcagc cgcgtgccgc
     2701 cggcttcggc gaccaggcag ttgccgccgc tgccgctggc gagggaagcg aagcgcagga
     2761 ttacttgagc tgctcgtgca gcagcgtcag gatgcgctgc gtggtcttcg tgttttcagt
     2821 ggcgccgtcc ttgttcagga cctggacctg gctgttctcg tccgcctggc gaacctgcac
     2881 gcgatattgc tcggccttga gcttggactc gccgccgaac agcttgccga agaagccgcg
     2941 gtcgcggtcc ttgatcgcca tttcgctttc cgggtcggcg tagcgcacga agtacaggcc
     3001 cttctgccgg tcgcggtcct cgacggtgaa gccgacccgg tcgagcacca gcccgacgcg
     3061 ccgccaggcg cggtcgaacg gctcgttcac ctgcagcagc tcggcgccgt cgatgccctt
     3121 gcggatctcg gcgcgctgcg actgcgggct ggctgcgacc aggtcctttg cgcgctcggc
     3181 ctggtagccc agccgcacca tgagccggcg caggaactcg gcctccaggc cgggatcggg
     3241 ctcgcgcggt gaccagacgg tctgcgcgtt ctccccggcc acgccgcgcg gcgaggtgta
     3301 cacctcgttc atgccgcggt ggctgatgta gacctcgctg cccttgccgt cggccgtgcg
     3361 ctccagccgc gtgcggaact tgtcgcgctc cgaggtcgag tagacctggt cgaggaactt
     3421 ggtgaaggtg tcgcgcagta cgccgcccgg caccttggcg cggttctccg cccagtcggt
     3481 ctccatcacg ccggcctccg gcaattcggt cttgatgagg aagccgttct cctgccagaa
     3541 atccttgacc atcggccaca ggcgttcggg cgtctcgttc gccaccacca gccagcgctc
     3601 cgagcccgcg cgctcgacgc gcatctgctc gacctgcggc agcacctgcg tgttgccggc
     3661 gcggctctgc tccttgcgct cggcctggta gcccgagagc gtggccgacg tgcgtccggt
     3721 ctccggcacg acgtagcggt tgtcgcgcgc cggcgaggtc aggtcgggcg gcacctccag
     3781 cgtcggcatg gtcttgccct tggcctgcga cttgtagtcg accttggagt cgagcatgtc
     3841 gtcgatcgag gcgcaggcga cgaggcacag ggtggtcgct gcaaccgcca actctcgctt
     3901 caagcgcggc cctcgaccac ccgcagcccg ggcagcgcga tgccggcttc gtgcaacgcc
     3961 tcgcgcacgg ctccctggaa cttgtccgac atcggcacga gcggcaggcg cagcccgttc
     4021 tcgatcatgc tcatctcggc cagcgcccac ttgaccgggg ccgggctggt ctcgatgaac
     4081 aggcgctgat gcagcatgag cagcttgagg ttgatctcgc gcgccttctt gacgtcgccg
     4141 accagcgcag cgccgcacat ctggtgcatc agcttggggg cgacgttggc ggtgaccgag
     4201 atcacgccgt ggccgcccag caggatcagc gccagcgccg tcacgtcctc gccgctgtag
     4261 atggcgaaat tgcgcggcat gcggcggatc agatcggtgc cgcgctcgat gttggcgctc
     4321 gcgtccttga tgccgatgat gcccggcacc tcggtaaggc gcagcacggt gtcgttctgc
     4381 atgtcggcga cggtgcgcgc cggcacgttg tacaggatca tcggcaggtc gaccgtctcc
     4441 gcgatcttgc ggaagtgctg gtacatgcct tcctgggtcg gctggttgta gtagggcacg
     4501 acggacagcg acgccgtggc gcccgccttc ttggccgatt cggtcagctc gatcgcctcg
     4561 gccgtggagt tgccgccggt accggcgatg atcgggatgc ggcccttgga gtgctgcaca
     4621 gcgatgcgaa tcagttcctt gtgctcgtcg aagtccaccg tcggcgattc gccggtggtg
     4681 ccgaccacga cgatgccgtc ggtgccctcg gcgacatgcc agtcgatgag cttgcggaac
     4741 gcagccaggt cgagcgcgcc gtcggcgcgc atcggtgtga cgattgctac caagcttccg
     4801 gtaatcatgg cgcgattcta atcctttcgg cccgcccccg cagggcgcaa agcctttgaa
     4861 tcatggccgc tttcggcctt tctaccctgc cctgctcgaa ggctgcgact tcgagccccg
     4921 cgaacgcctc gagcagctcg ccggggcgca gcagaaactc ggggttggac ggccggccga
     4981 atttttcgtt cccgaccatg aacgtctcgt agatcaggac gccgccctcg tcgagcgcgg
     5041 ccgcgagctg cgcaaacagc ggccggtgca gatagttcgt aacgacgatc gcggcgaagc
     5101 gttgacccgc gaacggccac ggattgccgt tttccaggtc ggctttcaca aacaggattt
     5161 cttttgaaag gacttggttt tcgcggtcga ccgccacgac ctgcataccc ctctcagaga
     5221 agaggcgcga atgccgcccg ccgccgcagg cgacgtcgag caccggcccg cgctcgacca
     5281 gcggcgccca gcgcaggacc cattcggatg ggtcttccat atttgtggca tcttagggaa
     5341 gtttcggggg atacgacaac gaggaggcgc tatggacaac cggccccgag tgctgattgt
     5401 cgacgactct aaagtagtgc gcaccacgct ggcaaaggtc atccgcagca ccttcgacgt
     5461 gcgggaggaa gccgacggcg aggccggctg ggcggcgatc gacggcgacc cggccatcgt
     5521 ggcggtgatc tcggacctcg gcatgcccaa gctcgacggc atgggcctgc tcgcccgcat
     5581 ccgcaactcg ccgcagccgc gcgtccgcga cctgccgttt ctcgtcatct ccggcaacga
     5641 ggacgacgac acccgcgacc gggcgcgcgc cgccggcgcc aacgatttca tctccaagtc
     5701 caccaagggc gccgaagcca tcgcccgcat cgacaacctg ctgcggctgg tgaaggcgaa
     5761 gcaagacctt gaagcgagcg cggatgcgct caaggtcagc gacgacgaca agatgtggga
     5821 ccagcccacc ggcgcgttca cctcgagcta cctgctcacc gagggcggca agcactactc
     5881 ccatgccaaa cgccatggca ctggactgtc ggcggtcagc ttccgcatcg acaactacag
     5941 cgacgtcgaa ggcaaggtcg gcaaggtgcc ggcagagcaa ctcctcgcgc gcatcgccaa
     6001 gctggtgcag gaaacgctac gcgccgagga ttcggtgggg cgcatgggtc ccgcactgtt
     6061 cagcgcgatc ctgtcgagca ccgcggccga gcaggcggt
//
LOCUS       DASYJA010000302         2331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1952537, whole genome shotgun sequence.
ACCESSION   DASYJA010000302 DASYJA010000000
VERSION     DASYJA010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1952537"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VGO02_06625"
     CDS             complement(<1..196)
                     /locus_tag="VGO02_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004543995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV7477394.1"
                     /translation="MHPMLSIAVRAARRAGSIINRAALDSGGLQIRSKKANDFVTEVD
                     RAAEAAIIEAVQKSYPEHAIL"
     gene            224..916
                     /locus_tag="VGO02_06630"
     CDS             224..916
                     /locus_tag="VGO02_06630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV7477395.1"
                     /translation="MNRIRIVLCRPSHPGNIGAAARAMKTMGLADLRLVAPERYPAPE
                     ADWMATNAVDVLKAATVHASLEDSIRDCVAAFAMSARPREWSPQVLDARGAAARAMAL
                     EGDVAFVFGNETAGLTNEEVFACQFLVHIPTSPEFNSLNLAQAVQVMAYELYVVQNPT
                     GLTARSEKAATVADLEGLYAHLEEAARQTNFMASGSRLRERWRRLFSRVPALEREEVN
                     IMRGLLRALLGK"
     gene            953..1669
                     /gene="cysE"
                     /locus_tag="VGO02_06635"
     CDS             953..1669
                     /gene="cysE"
                     /locus_tag="VGO02_06635"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEV7477396.1"
                     /translation="MFTKLRADIACILDRDPAARSTWEVLTCYPGLHAIYIHKLAHWF
                     WLRGLRWLGRFTSHLGRWLTGIEIHPGAKIGSCVFIDHGMGVVIGETAEVGDGCTIYQ
                     GVTLGGTSLYRGEKRHPTLGAGVVVGAGAKILGGFTVGDGARIGSNAVVVKAVSAGAT
                     VVGIPGKIVEDPGTKSAERFAAYAVVEDHDDPQSKAIHQLVEHSRELERTIEILQDRL
                     ARLERQEAAAETDKNLRAVK"
     gene            1750..2136
                     /gene="iscR"
                     /locus_tag="VGO02_06640"
                     /pseudo
     CDS             1750..2136
                     /gene="iscR"
                     /locus_tag="VGO02_06640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02010.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
BASE COUNT          435 a          749 c          761 g          386 t
ORIGIN      
        1 cgaggatcgc gtgctcggga taggacttct gcaccgcctc gatgatcgcc gcctccgccg
       61 cacggtcgac ctcggtgacg aagtcgttcg ccttcttcga cctgatctgc aggccgccgc
      121 tgtcgagcgc ggcgcggttg atgatcgagc cggcgcggcg tgcggcgcgc acggcgatac
      181 tgagcatggg gtgcatgggg gcggtattct agactgtggc tctatgaatc gaattcgcat
      241 cgtcctgtgc aggcccagcc accccggaaa catcggcgcc gccgcgcgcg cgatgaagac
      301 gatgggcctt gccgacctgc gcctggtcgc gcccgagcgc tatccggcgc cggaagccga
      361 ttggatggct acgaacgccg tggatgttct taaagcggcc accgtgcatg cttcactgga
      421 agattcaatc cgggattgcg tcgcggcgtt cgccatgtcg gcgcggccgc gcgagtggtc
      481 gccgcaggtg ctcgatgcgc gcggcgccgc ggcgcgcgcg atggccctgg agggcgacgt
      541 ggctttcgtc ttcggcaacg agacggctgg attgaccaac gaggaagtct tcgcctgcca
      601 gttcctggtc cacattccga ccagccccga attcaactcg ctgaacctcg cgcaggcagt
      661 gcaggtgatg gcgtatgagc tctacgtggt gcaaaacccc acgggactca ccgcgcgaag
      721 cgagaaagcg gcgacggtag cggaccttga agggctctat gcccatctcg aagaggcggc
      781 acggcagacc aacttcatgg cgagcggatc gcgcctgcgg gagcgctggc gccggctttt
      841 ctcgcgcgtg cccgcgctcg agcgcgagga ggtcaacatc atgcggggcc tcctgagggc
      901 gctcctcggc aaatagttga tcaaaacagt cggaaaccct aatatggcct ttatgttcac
      961 gaagctgcgc gccgacatcg cctgcatcct cgaccgggac cccgcggcgc ggagcacatg
     1021 ggaagtgctc acctgctatc cggggctcca tgcgatctat atccacaagc tcgcccactg
     1081 gttctggctc cggggcctgc gctggctcgg gcgcttcacc tcgcatctcg gccgctggct
     1141 gaccggcatc gagatccatc ccggcgcgaa gatcggaagc tgcgttttca tcgatcacgg
     1201 catgggcgtg gtcatcgggg aaaccgcgga agtgggcgac ggctgcacca tctaccaggg
     1261 cgtcaccctg ggcggcactt cgctttatcg gggtgaaaag cgccatccca ccttgggcgc
     1321 tggagtcgtg gtgggcgcag gcgccaagat cctcggcggt ttcacggtgg gggacggtgc
     1381 gcgcatcgga tccaacgcgg tagtcgtgaa ggccgtctcg gcaggcgcca cggtcgtggg
     1441 aattcccggc aagatcgtcg aagatcccgg cacgaagagc gcagaacgct tcgcggccta
     1501 tgcggtggtc gaggaccacg acgacccgca gtccaaggca atccaccagc tcgttgagca
     1561 ctcccgggag ctcgagcgca cgatcgagat tctccaggac cggctcgccc gcctcgagcg
     1621 tcaggaagcc gccgccgaga ccgacaaaaa cctgagagcg gtcaaataat tagtacctga
     1681 gggtttcagt cggctttggg ttgcggtata cttgagcatc tttatcgggt attggacacc
     1741 ggacagcaca tgagactcac aaccaaaggc aggttcgcag tgaccgcgat ggtcgacctg
     1801 gcgatgcgcc agaaccgcgg cccggtcacg ctcgcggcga tcagcgagcg tcagcacatc
     1861 tcgctttcct acctcgaaca gctcttcggc aagctgcggc gcgcgaagct ggtatccagc
     1921 gtgcgcggcc ccggcggcgg ctacaacctg gcccagccga cgcagtcggt cagcgtcgcg
     1981 aacatcgtca gggccgtgga cgagccgctc gacgcgaccc aatgcggcgg caaggagaac
     2041 tgccacgacg aaaagcgctg catgacccac gatctctggg cgaccctgaa cgagaagatg
     2101 cacgactacc tctcgtcggt gagcctcgcc gacctcgtgg cgcaccagag cggcaagccc
     2161 gtcgccgtga tcaaggattt caggcagcac gagaagtcgc acgacaaacc gcagcacgac
     2221 aagccggccg aggagaaggt ggccgcatta ggctgccgat ctatctcgac tactcggcaa
     2281 ccacgccggt cgatccgcgg gtcgccgaga agatgatccc gtacctcacc g
//
LOCUS       DASYJA010000303         5452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2133339, whole genome shotgun sequence.
ACCESSION   DASYJA010000303 DASYJA010000000
VERSION     DASYJA010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2133339"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VGO02_06645"
     CDS             <1..219
                     /locus_tag="VGO02_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477397.1"
                     /translation="DLAVLVASRISGVAGVQLVGAIPQELQTWIGFTAGASARATHPE
                     AARAMLRFFTAPGSAAVLRINGVEPFVE"
     gene            232..822
                     /locus_tag="VGO02_06650"
     CDS             232..822
                     /locus_tag="VGO02_06650"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEV7477398.1"
                     /translation="MRFVPLLGVLLLIALAFFWRPWLQYQRHGTWGVILFRGGSWGQR
                     LRDATLVVFTLLVLGQAVVAAARPELVADHILLQAAGIVPLFGGVALVVIAQLQMGVS
                     WRIGIDEKAAPGLVTTGIFRFCRNPIYLAMIIVTAGYALLLPTLLSFVLLAGTIAGMS
                     TQARTEEAYLLKTYGDSFRDYARRVGRFLPGIGKLH"
     gene            832..1167
                     /locus_tag="VGO02_06655"
     CDS             832..1167
                     /locus_tag="VGO02_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477399.1"
                     /translation="MRLPAVLLASLLSLPAQGDSRLPDPDDDDPYYDPWTVEERAWND
                     HRRPPEYSPAPRYSDTADCDAAFERYRESIDCFARFRNQNGSVRAEAYRYCSPVLDPS
                     PRCGPPRRW"
     gene            complement(1177..1785)
                     /locus_tag="VGO02_06660"
     CDS             complement(1177..1785)
                     /locus_tag="VGO02_06660"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEV7477400.1"
                     /translation="MTATARAVSVAHFDLPALPYAYDALEPVISKETLELHHDKHHRK
                     YVDTMNELLQKENVHAGSLEAVVKHSSGKLFNNSGQAWNHNFYWQSLSPRGGKPSQAM
                     LGHIAKAFGDWDKFAKAFAAAANNQFGSGWAWLVNRHGKLDIMATPNAESPMAQGVPC
                     LLTLDVWEHAYYVDYRNQREKYVEALINERLNWAFAEKNFGG"
     gene            complement(1799..2860)
                     /locus_tag="VGO02_06665"
     CDS             complement(1799..2860)
                     /locus_tag="VGO02_06665"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEV7477401.1"
                     /translation="MFDAMAAARDHISLETYILDGGEIGERLAALLAKKVDEGVKVNL
                     LYDSVGSIKTPPEYFERLRAAGVAVCEFNPASDLLRVNNRDHRKILVVDGRVAFTGGI
                     NISQAYAMGSGRARRTPARETREGWRDTQVQAEGPVVADFQRLFLDAWALQDCGPAGP
                     AAYFPRPARGGDMAMRAMRAEPGGDAAEMYDALLTQIGGARSRVWLTVGYFVPDPGIK
                     QALIQAARRGVDVRLVLPGYSDFWAPVYAGRSHYAELLAAGVHIHEWREALMHAKTAV
                     IDSKWSSIGSTNLDWRSFVHNYEADLVVVDAAFARDMEERFRRDVAAATAIDPAAWKL
                     RGAGEKVKEWIARRWEYLL"
     gene            complement(3034..3354)
                     /locus_tag="VGO02_06670"
     CDS             complement(3034..3354)
                     /locus_tag="VGO02_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEV7477402.1"
                     /translation="MNKVREAICAAIAVAAIGGFAACSSDPAKKETVGEYAGDAGLTA
                     KVKTAIATDVGARTAAGVNVETYKGTVQLSGFVDSQDQATRAADAAKKVEGVRSVKND
                     IRVK"
     gene            complement(3452..4237)
                     /locus_tag="VGO02_06675"
     CDS             complement(3452..4237)
                     /locus_tag="VGO02_06675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV7477403.1"
                     /translation="MRDLDLDEQVPVMAWTARPDMSCESLSRQWLAFTGYSAEQALGD
                     GWSRGVHPEDLGRWLDTCVRAFDSREPFEIEYRLRRRDGEYRWMLDRGVPRYGRDGRF
                     LGYAGVCVDIDVRKRAERELERALERERSLRLAAEEAGRLKDRFIAAALREAIERGGP
                     VQLRTILAGVRVLVVDGDAGAREQMAKVLGVAGAETKTAARAAEPLGTWRPDVVISDI
                     GLRGGSLLVRALRGAGRDAQLAKPVEALALLATVARVAQPAAV"
     gene            4437..4601
                     /locus_tag="VGO02_06680"
     CDS             4437..4601
                     /locus_tag="VGO02_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010120894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HEV7477404.1"
                     /translation="MLSWALAFFIVAIIAAVFGFTGIAAGAAEIAKILFVVFVVLFLV
                     SLVAGLLRRP"
     gene            4623..5066
                     /locus_tag="VGO02_06685"
     CDS             4623..5066
                     /locus_tag="VGO02_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477405.1"
                     /translation="MKFQLMSIAVAMAFAVGAQAQSTSTRQVKNADEDRIEAEYKAAR
                     ERCDPMQGNAKDICQKEAKGKEKVAKAELEAKTNPTAANQRKVQEAKADAAYDVAKER
                     CDDKKANDKDVCQKDAKAAHERAMADIKRADAKSAGTGSTKATSK"
     gene            5066..>5452
                     /locus_tag="VGO02_06690"
     CDS             5066..>5452
                     /locus_tag="VGO02_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEV7477406.1"
                     /translation="MAGTHLSDIQTLRKRAREHIAQGAVTPGYHADRGVVLKLLNEAL
                     ATEIVCTLRYRRHYFMAKGIHAEGVAAEFLEHANEEQHHADQIAQRIVQLGGEPDFSP
                     DGLSSRSHAEYVEGETLEDMIKEDLVA"
BASE COUNT          858 a         1889 c         1830 g          875 t
ORIGIN      
        1 gacctggcgg tgctcgtcgc ctcgcggatt tccggcgtgg ccggcgtgca gctggtcggc
       61 gccatccctc aggagctgca gacctggatc gggtttaccg ccggcgcgag cgcccgcgcc
      121 acgcatcccg aggcggcgcg cgccatgctg cgcttcttca ccgcacccgg ctcggcggcc
      181 gtgctgcgca tcaacggcgt cgagccgttc gtcgaatgac gcgcggctag cttgcgcttc
      241 gttccgctgc tcggcgtgct gctgctgata gcgctcgcgt tcttctggcg gccctggctg
      301 cagtaccagc gccacggcac ctggggggtg atcctgttcc gcggcggcag ctggggccag
      361 cgcctgcgcg acgctacgct ggtggtattc actctgctgg ttctcgggca ggcagtcgtc
      421 gccgccgcgc ggcccgagct ggtcgcggac cacatcctgt tgcaggctgc gggaatcgtg
      481 ccgctgttcg gcggcgtcgc cctggtcgtc atcgcccagc tgcagatggg ggtttcatgg
      541 cgcatcggca tagacgagaa agctgcgccg ggcctggtga cgacgggaat cttccgcttc
      601 tgccgcaacc cgatctacct ggcgatgatc atcgtgaccg ccggctacgc gctgctcctg
      661 cccaccctcc tctccttcgt cctgctggcc ggcacgatcg ccggcatgtc gacgcaggcg
      721 cgcaccgagg aggcgtacct gctgaagacc tacggcgaca gctttcgcga ctacgcgcgc
      781 cgggtcgggc gcttcttgcc gggaatcggc aagctgcact aggctgaccg catgcgcctg
      841 cccgccgtcc tgctcgccag cctgctgagc ctgcccgccc agggcgactc tcgcctgccg
      901 gacccggacg acgacgaccc ctattacgac ccgtggaccg tcgaggaacg ggcttggaac
      961 gatcaccgcc ggccgccgga atatagtccc gcgccgcgct actccgacac ggccgattgc
     1021 gacgcggcgt tcgagcgcta ccgcgaaagc atcgactgct tcgcgcggtt ccgcaaccag
     1081 aacggcagcg tcagggccga ggcgtaccgc tattgcagcc cggtgctcga cccttcgccg
     1141 cgctgcggcc cgccgcggcg ctggtagagg gcgctgtcac ccgccgaagt tcttctcggc
     1201 gaacgcccag ttcagccgct cgttgatcag cgcctcgaca tacttctcgc gctggttgcg
     1261 atagtcgacg tagtaggcgt gctcccagac atcgagtgtc agcaggcagg gaacgccctg
     1321 cgccatcggc gactcggcgt tgggcgtggc catgatgtcg agcttgccgt ggcgattcac
     1381 cagccaggcc cagccgctgc cgaactggtt gttggcggcg gccgcgaacg ccttggcgaa
     1441 cttgtcccag tcgccgaagg ccttggctat gtggccgagc atcgcctgcg agggcttgcc
     1501 acccctgggg gaaagcgact gccagtagaa attgtggttc cacgcctggc ccgaattgtt
     1561 gaacagcttg cccgagctgt gcttgaccac ggcctcgagc gagccggcgt gcacgttctc
     1621 cttctgcagc agctcgttca tggtgtcgac gtacttgcgg tggtgcttgt cgtgatgcag
     1681 ctccagcgtc tctttcgaga tcaccggctc cagcgcatcg taggcgtagg gcagcgccgg
     1741 caggtcgaaa tgggcgacgc ttacggctct tgcagtggca gtcatcggtt tcctttcgtc
     1801 aaagcagata ttcccagcgg cgggcgatcc attccttcac cttctccccg gctccccgca
     1861 gtttccatgc cgccgggtcg atggcagtgg cggctgccac gtcgcgccgg aagcgctcct
     1921 ccatgtcgcg cgcgaacgcg gcatcgacga ccaccaggtc ggcctcgtag ttgtgcacga
     1981 agctgcgcca gtcgaggttg gtcgagccga tgctcgacca cttcgagtcg atcaccgcgg
     2041 tcttggcgtg catcagggcc tcgcgccatt catggatgtg cacgcccgcg gcaagaagct
     2101 ccgcgtagtg cgagcgcccg gcgtacaccg gcgcccagaa atcgctgtag ccgggcagca
     2161 ccagccgcac gtcgacgccc cggcgggcgg cctggatcaa tgcctgcttg atcccggggt
     2221 cggggacgaa gtagccgacg gtgagccata cgcggctgcg cgcgcccccg atctgcgtga
     2281 gcagggcgtc gtacatctcg gcggcatcgc cgcccggctc ggcgcgcatc gcccgcatcg
     2341 ccatgtcgcc gccgcgcgcg ggccggggaa aataggccgc cggcccggcc gggccgcagt
     2401 cctgcagcgc ccacgcgtcc aggaacaggc gctggaaatc ggccaccacc ggcccctcgg
     2461 cctgcacctg cgtatcgcgc cagccttccc tggtttccct ggcgggcgtg cggcgggcgc
     2521 ggccggaacc catcgcatag gcctggctga tgttgatgcc gccggtgaag gccacgcgcc
     2581 cgtcgaccac caggatcttg cggtggtcgc ggttgttgac gcgcaaaagg tccgaggccg
     2641 ggttgaattc gcacaccgcg acgcccgccg cgcgcagccg ctcgaagtac tcgggcgggg
     2701 tcttgatcga gccgacgctg tcgtacagca ggttgacctt gacgccctcg tcgaccttct
     2761 tggcgaggag cgcggccagc cgctcgccga tctcgccgcc gtcgaggatg taggtctcga
     2821 ggctgatgtg atcgcgcgcc gccgccatcg cgtcgaacat cgcgcgatgc gtgcgcggcc
     2881 cgtcgatgag aatgcgcccg cggttgcctt cgaccagggg ctgcccgggc gccggcgcgc
     2941 gctcgatggc gggcagcggc gcgcaggcgg ccagaacgag cgctacgaga accgccgcgc
     3001 ggcgtgttgc gtggcgtgtt gcgtggcgtg ttgctatttg acgcgtatgt cgttcttgac
     3061 ggaacgcacg ccctcgacct tcttggctgc gtcagccgcc cgcgtcgcct gatcctgcga
     3121 gtcgacgaag ccggagagct gcaccgtgcc cttgtaagtc tccacattga cgccggccgc
     3181 ggtgcgcgcg ccgacgtcgg tggcgatcgc cgtcttcacc ttggcggtga ggccggcgtc
     3241 gccggcgtac tcgcccacgg tctccttctt cgccgggtcg gacgagcagg cggcgaaacc
     3301 gccgatcgcg gcgacagcga tcgccgcgca aatagcttca cgtactttgt tcattgcagt
     3361 ctcctaggtc gtggaacgga aaaaatcgaa cggctaacgg agaacagcaa accgcgtgcc
     3421 ggtagcactt tctgccctac aagggaactt cttacacggc ggccggctgg gccacgcgcg
     3481 cgacggtggc aaggagcgcg agggcctcga ccggcttggc gagctgggcg tcgcgcccgg
     3541 cgccgcgcag cgccctcacc agcagcgaac cgccgcgcag accgatgtcg gaaatcacta
     3601 catcggggcg ccaggtgccg agcggctcgg cggcgcgcgc ggcggtcttg gtttccgcgc
     3661 ccgcgacgcc aagcaccttg gccatttgct cgcgcgcgcc ggcgtcgccg tcgaccacca
     3721 gcacccgcac gccggcgagg atggtgcgca gctgcaccgg gccgccacgc tcgatcgcct
     3781 cgcgcaacgc ggcggcgatg aagcgatcct tcagccggcc ggcctcctcg gcggccaggc
     3841 gcaggctgcg ctcgcgctcc agcgcgcgct caagctcgcg ctcggcgcgc ttgcgcacgt
     3901 cgatgtcgac gcacacgccg gcgtagccaa ggaagcggcc gtcgcgcccg tagcgcggca
     3961 cgccgcggtc gagcatccag cggtactcgc cgtcgcggcg ccgcaggcgg tattcgatct
     4021 cgaagggctc gcgcgagtcg aacgcgcgca cgcaggtgtc gagccagcgg ccgaggtcct
     4081 ccgggtgcac gccgcgcgac cagccgtcgc cgagggcctg ctcggcgctg tacccggtga
     4141 aggcgagcca ttgccggctg agcgactcgc acgacatgtc ggggcgcgcc gtccacgcca
     4201 tcaccggtac ttgctcgtcg agatctaggt cgcgcatcgg atctttcagc gctcaccctg
     4261 caggagccat gccgcggcgc gctgtagagg aagcgctacg ccgcgtcgct tcgcagcggc
     4321 cggaatttcc tacaaacggc ggcaagcatt acagcgcggc gcgcggtcca atctcggcat
     4381 ggagtttgct caagggtgat ttgctttcac cacccattca tcgcaaggag tccgtcatgc
     4441 ttagctgggc gctcgcattc ttcatcgtag caatcattgc cgccgtattc ggcttcaccg
     4501 gcatcgccgc cggcgccgcc gagatcgcga agatcctgtt cgtagtcttt gtggtcctgt
     4561 tcctggtctc gctggtcgcc gggctgctga gaaggcctta atccgttaac aaggagatcg
     4621 acatgaaatt ccaactgatg agcattgcgg tggccatggc cttcgccgtc ggcgcgcagg
     4681 cacagagcac ttccacccgc caggtcaaga acgccgacga agaccgcatc gaggccgagt
     4741 acaaggccgc gcgcgagcgc tgcgacccga tgcagggcaa cgcgaaggac atctgccaga
     4801 aggaagccaa gggcaaggag aaggtcgcca aggccgagct cgaggcgaag accaacccca
     4861 ccgcggccaa ccagcgcaag gtgcaggaag cgaaggccga tgccgcctat gacgtcgcga
     4921 aggagcgctg cgacgacaag aaggccaacg acaaggacgt ctgccagaag gatgccaagg
     4981 cggcacacga acgcgccatg gccgacatca agcgcgccga cgcgaaaagc gccggcaccg
     5041 ggtcgacgaa ggcgacgagc aagtaatggc cggaacgcac ctgtccgaca tccagacgct
     5101 gcgcaagcgc gcccgggagc acatcgcgca gggcgcggtg actcccgggt accacgccga
     5161 ccgcggcgtg gtgctcaagc tgctgaacga ggcgctcgcc acggagatcg tctgcacgct
     5221 gcgctaccgg cgccactact tcatggcgaa gggcatccat gccgagggcg tggcggccga
     5281 gttcctcgag cacgccaacg aggagcagca ccatgccgac cagatcgcgc agcgcatcgt
     5341 gcagctgggc ggcgagcccg acttctcccc cgacggcctg tcgagccgca gccatgcgga
     5401 atacgtcgag ggagagacgc tcgaggacat gatcaaggaa gacctggtcg cc
//
LOCUS       DASYJA010000304         3130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_317754, whole genome shotgun sequence.
ACCESSION   DASYJA010000304 DASYJA010000000
VERSION     DASYJA010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317754"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VGO02_06695"
     CDS             complement(<1..297)
                     /locus_tag="VGO02_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEV7477407.1"
                     /translation="MKAMILAAGRGERLRPLTERLPKPLVEAGGKPLIDWHLERLAAA
                     GWREAVINVSHLAERIVAHVGDGARYGMRLAWSREAEPLETAGGIAKARHQLGDA"
     gene            complement(294..1280)
                     /locus_tag="VGO02_06700"
     CDS             complement(294..1280)
                     /locus_tag="VGO02_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV7477408.1"
                     /translation="MIHGDKRRAQLERWLAAQLPGKHLSLTPASEDASFRRYFRVALQ
                     DGGSLIAMDAPPEKEDCRPFVQVAQLLQDAGVHAPQVRAQDLAQGFLLLTDLGTRSYL
                     QELTGQNAAENAGRLFADATDALVKWQLASRAGVLPDYDEALLRRELNLFPEWYVQKH
                     LGKTVSKETLESIFAVLVKSALAQPAVYVHRDYMPRNLMVCEPNPGVLDFQDAVFGPI
                     TYDPVCLMRDAFISWQEEQVLDWTVRYWEKAKQAKLPVDADFGEFWRAFEWMGLQRHL
                     KVLGIFARLNHRDGKPKYLADTPRFIGYALSVSKRYRELGPLARLLDELHPA"
     gene            1321..>3130
                     /gene="lptD"
                     /locus_tag="VGO02_06705"
     CDS             1321..>3130
                     /gene="lptD"
                     /locus_tag="VGO02_06705"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEV7477409.1"
                     /translation="MPFRLATLALALAIAAPVFAQAQKKPAVQEPTTIDAENIEGVSD
                     IEVTARGNAEIRRGDTVIFGNVLRFNRELGRAEGDGGVRLQDGVDRFFGPRMQYNTLD
                     GTGFFEQPTFLLQRENEARGAAERVEFLGKDKFRFFNAHYTTCRPGQEDWVLTARELE
                     LDYEKDEGRAKSPRLRFFDVPIIGVPAASFPLESKRRSGLLAPYYSQSSQRGFEVAAP
                     YYWNIAPEYDDTITPVYMTKRGTQLKNQFRYLSPKYSGELKFEYLPDDKVFRETREGV
                     SLQHTQTFFSTGQPGTSIGGTSLTANVDYNRVSDDKYFVDLASQVRQITIGNLPQDAY
                     LTYNGLAGGAPYSAQARVQRFQTLQDPLAPIVPPYARLPQLTFSGSYGNLGGFLDTTL
                     PVEYVYFSHPTLVEGARSTLSPTFATPIVAPWGFFTPKVGLRYVDYNLTNNTVPGQET
                     APSASVPWFSADTGLLFERPINLFGQARTQTLEPRLFYVYVPYRNQDAIPLFDTALAD
                     LNYQQLFSENRFVGGDRFGDANQMTIALTSRLLQANGQEGLRATIAQRHYFQDERVGL
                     TPTSTLRTTRDSDLLASIGGRPSRAWAFDVTTQWGQQ"
BASE COUNT          538 a         1098 c          985 g          509 t
ORIGIN      
        1 cgcgtcgccg agctggtggc gcgccttggc gatgcccccg gcggtctcca gcggctcggc
       61 ttcgcgcgac caggcgaggc gcatgccgta gcgcgcgccg tcgccgacgt gcgccacgat
      121 ccgctcggcg aggtgcgaca cgttgatgac cgcctcgcgc cagccggcgg ccgcgagcct
      181 ctccaggtgc cagtcgatga ggggcttgcc gccggcttcg accagcggct tgggcaggcg
      241 ctcggtgagc ggccgcagcc gctcgccgcg gccggcggcg aggatcattg ccttcatgcg
      301 ggatgcagct cgtcgagcag tcgcgccagc ggacccagtt ctctgtatct tttggaaact
      361 gaaagagcgt agccgatgaa gcgcggcgtg tccgccaggt atttcggctt gccgtcgcgg
      421 tggttgagcc gcgcgaagat gccgagcacc ttgaggtggc gctgcagccc catccactcg
      481 aacgcgcgcc agaactcgcc gaaatcggca tcgaccggga gcttcgcctg cttcgccttc
      541 tcccagtacc gcacggtcca gtcgaggacc tgctcttcct gccagctgat gaaggcgtcg
      601 cgcatcaggc agacggggtc ataggtgatc ggaccgaaca ccgcgtcctg gaagtcgagc
      661 accccgggat tgggctcgca caccatcagg ttgcgcggca tgtagtcgcg atggacgtag
      721 accgcgggct gggcgagcgc gcttttcacc aggacagcga agatgctttc gagagtttct
      781 ttcgaaaccg tttttccaag atgcttctgc acgtaccact ctggaaacag gttcagctcg
      841 cgccgcagga gcgcctcgtc gtagtccggc aacacgccag ccctgctggc gagttgccac
      901 ttgaccagcg cgtcggtggc gtcggcgaac aggcgcccgg cattctccgc cgcattttgc
      961 ccggtgagct cctgcaggta gctgcgcgtg ccgaggtcgg tgagcaggag gaagccctgg
     1021 gcgagatcct gcgcccggac ctgcggagca tgcacgccgg cgtcctgcag gagctgcgcc
     1081 acctgcacga agggtcggca gtcttccttc tccggcggcg cgtccatcgc gatcaggctg
     1141 ccgccatcct gcagcgcgac gcggaaatag cgccggaagc tggcgtcctc ggaagcaggg
     1201 gtgagagaca ggtgtttacc gggaagctgg gcggccagcc agcgctcgag ctgagcgcgt
     1261 cgtttgtcgc cgtgtatcat tgaaaattct aacatcaaca gaaagactta cggccgctcc
     1321 atgccgttcc gccttgcgac gctggccctc gcccttgcca tcgccgctcc ggttttcgcg
     1381 caggcgcaaa aaaaacccgc cgtgcaggag ccgacgacca tcgacgccga aaacatcgag
     1441 ggcgtcagcg acatcgaggt gacggcgcgc ggcaacgccg agatccggcg cggcgacacg
     1501 gtcattttcg gcaacgtgct gcgcttcaac cgcgagctgg gccgcgcgga aggcgatggc
     1561 ggcgtgcggc tgcaggacgg cgtcgaccgc ttcttcggcc cgcgcatgca gtacaacacg
     1621 ctggacggca ccggcttctt cgagcagccg accttcctgc tgcagcgtga aaacgaggcc
     1681 cgcggcgccg ccgagcgcgt cgagttcctc ggcaaggaca agttccgctt cttcaacgcc
     1741 cactacacca cctgccgtcc cgggcaggag gactgggtgc tgaccgcgag agagctcgag
     1801 ctcgactacg agaaggacga aggccgcgcc aagagcccgc ggctgcgttt cttcgacgtg
     1861 ccgatcatcg gcgtgccggc agcctcgttt cccctggaga gcaagcggcg cagcggcctg
     1921 ctcgccccgt actactcgca gagcagccag cgcggcttcg aggtcgcggc gccctactac
     1981 tggaacatcg cgccggagta cgacgacacc atcaccccgg tgtacatgac caagcgcggc
     2041 acccagctga aaaaccagtt ccgctacctc agcccgaaat actcgggcga gctcaagttc
     2101 gaatacctgc ccgacgacaa ggtattccgc gagacgcgcg aaggggtgtc gctccagcat
     2161 acgcagacct tcttcagcac cggccagccc ggcaccagca tcggcggcac cagcctgacc
     2221 gcgaacgtcg actacaaccg ggtatccgac gacaagtatt tcgtcgacct ggcgagccag
     2281 gtccggcaga tcaccatcgg caacctgccg caggacgcgt acctcaccta caacggcctc
     2341 gccggcggcg ccccatacag cgcgcaagcg cgcgtgcagc gcttccagac cctgcaggat
     2401 ccgctggcgc cgatcgtgcc gccgtatgcg cgcctgccgc agctcacctt cagcggcagc
     2461 tacggcaatc tgggcggctt cctcgacacg acgctgccgg tggagtacgt ctacttttcg
     2521 catccgacgc tcgtcgaagg tgcccgctcg acgcttagtc caactttcgc cactccgatc
     2581 gtcgccccct ggggcttttt tacgccaaag gtcgggctgc gctacgtcga ctacaacctg
     2641 accaacaata cggtgcccgg gcaggagact gcgccctccg cctcggtccc gtggttcagc
     2701 gccgataccg ggctgctctt cgagcgcccc atcaacctct tcggccaggc gcgcacccag
     2761 acgctcgagc cgcggctgtt ctacgtctat gtgccctacc gcaaccagga tgcgatcccg
     2821 ctcttcgaca ccgcgctcgc cgacctgaac tatcagcagc tgttcagcga gaaccgcttc
     2881 gtcggcggcg accgcttcgg cgacgcgaac cagatgacca ttgcgctcac ctcgcgcctg
     2941 ctgcaggcga acggccagga agggctgcgc gccaccatcg cccagcggca ttacttccag
     3001 gacgaacgcg tcggcctgac gcccacctcc acgctgcgca cgacgcgcga ttccgacctc
     3061 ctcgcttcga tcggcgggcg gccgtcgcgg gcctgggctt ttgatgtcac cacgcaatgg
     3121 ggccagcagc
//
LOCUS       DASYJA010000305         1941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1135562, whole genome shotgun sequence.
ACCESSION   DASYJA010000305 DASYJA010000000
VERSION     DASYJA010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135562"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VGO02_06710"
     CDS             <1..235
                     /locus_tag="VGO02_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEV7477410.1"
                     /translation="TTHPAKMLGLQGEVGNLKPGAAADVSVLHDQRGRWTLRDNEGNT
                     VRTERLLRPHFCLRMGARHDADASILPPLAEAA"
     gene            232..>1941
                     /locus_tag="VGO02_06715"
     CDS             232..>1941
                     /locus_tag="VGO02_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEV7477411.1"
                     /translation="MTGKGVGARLPRKEDRRFLLGQGEYVANIRLPGMLEVAFVRSPM
                     AHATILGITKPPGLETLVFTAQELDGVKPIRAVSALKGFKPSEQPILARDKVRQVGEL
                     LAMCVAPTRAEAEDLADATEVALEELPAVVDMLEARMNPPALVHEEWGDNVFLESLVD
                     DDLAPIKKQAAVTVTRKIRTARQHMSPMEGRGVVCEWNRRLGQLVMYSAAQMPHLNRA
                     GLAECLGIDQGAIRVIAPDVGGGFGYKGILLPEEVALAWLCRRLGRPVRWIEDRREQL
                     SANANCREHHYDLTLYADAGGKILGIDCEATVDSGAYSSYPFSACLEAAQVSSILPGP
                     YKMDRYRCRTWSVATNKAPILPYRGVARTGVCFALELMVDAVARELKMEPHEVRLANL
                     VQPGEMPYDNITKKHFDSGDYPEAVRRAARMIDVSGIRKLQKEPQGNRRVGVGFSIYC
                     EQAAHGTSVYYGWGIPMVPGHEQCSARLSPDGVLELRIGAHSHGQGMETTLAQVANTV
                     LGIDPNAVRLIHGDTALTPYSTGTWGSRSMVMSGGAVAAACEVIAGRIKAIASKLLEV
                     ASSD"
BASE COUNT          349 a          659 c          637 g          296 t
ORIGIN      
        1 gaccacccac ccggcgaaga tgctcggcct gcagggcgag gtcggcaatc tcaagcccgg
       61 cgccgccgcc gacgtgagcg tgctgcacga ccagcgcggc cgctggaccc tgcgcgacaa
      121 cgagggcaac acggtgcgca ccgagcgtct gctgcggccg catttctgcc tgcgcatggg
      181 cgcgcgccac gacgcggacg cctcgatcct gccgccgctc gcggaagcgg cgtgaccgga
      241 aagggtgtcg gagcgcggct tccgcgcaag gaagaccggc gcttcctgct gggtcaaggc
      301 gagtacgtcg ccaacatccg gctcccggga atgctggaag tcgctttcgt gcgatctccc
      361 atggcgcacg caaccatcct gggaatcacg aagccgcccg gcctggagac cctggtcttt
      421 accgctcagg agctcgacgg cgtcaagccg attcgcgccg tctccgccct gaaaggcttc
      481 aagccatccg agcagccgat tctcgcgcgc gacaaggtgc gccaggttgg cgagctgctg
      541 gcgatgtgcg tcgcaccgac ccgggccgag gcggaggatt tggcggacgc aaccgaagtc
      601 gctcttgaag agcttcccgc ggtcgtcgac atgctggaag caaggatgaa cccgccggcg
      661 ctggtgcacg aggaatgggg cgacaacgtc ttcctcgaat ccctcgtcga cgacgatctt
      721 gctccaatca agaagcaagc tgccgtgacc gtcacacgga agataagaac tgcacgccag
      781 cacatgtcgc cgatggaagg ccgcggcgtg gtgtgcgagt ggaaccggcg gctcgggcag
      841 ctcgtgatgt acagcgcggc gcagatgccg cacctcaacc gcgccggcct cgccgagtgc
      901 ctcggcatcg accagggcgc gatccgcgtc atcgcgcccg acgtcggcgg cggcttcggc
      961 tacaagggca tcctgctgcc cgaagaggta gccctcgcgt ggctgtgccg gcgcctcggc
     1021 cgcccggtgc gctggatcga ggatcggcgc gagcagctct cggcgaacgc gaactgccgc
     1081 gagcaccact acgacctcac gctgtacgcc gacgcaggag gcaagatcct cggcatcgac
     1141 tgcgaggcga cggtcgactc cggcgcgtat tcctcctacc ccttctcggc ctgcctcgag
     1201 gccgcgcagg tgagctcgat cctgcccggc ccctacaaga tggaccgcta ccgctgccgc
     1261 acctggtcgg tcgccaccaa caaggcgccg atcctcccct accgcggcgt ggcgcgcacc
     1321 ggcgtgtgct tcgccctcga gctgatggtc gacgcggtgg cgcgcgagct gaagatggag
     1381 ccgcacgaag tgcgcctcgc caacctggtg cagcccggcg agatgccgta cgacaacatc
     1441 acgaagaagc acttcgacag cggcgactat cccgaggcgg tgcggcgcgc tgccaggatg
     1501 atcgatgttt ccgggatcag aaaactccag aaggaaccgc agggcaaccg aagggtcggt
     1561 gtgggatttt ccatttattg cgaacaggcg gcgcatggca cctcggtcta ctacggctgg
     1621 ggcatcccga tggtccccgg ccacgagcag tgctcggcgc gcctctcgcc cgacggcgtc
     1681 ctcgagctgc gcatcggcgc gcactcgcac ggccagggca tggaaacgac cctcgcccag
     1741 gttgcaaata ctgttttggg aatagatcca aatgctgtca ggttgataca cggcgacact
     1801 gccctcaccc cctactccac cggcacctgg ggctcgcgca gcatggtgat gtccggcggc
     1861 gccgtcgccg cggcatgtga agtgattgca gggcggataa aagcaattgc ctcaaaactt
     1921 ctcgaagttg cgagtagcga c
//
LOCUS       DASYJA010000306         1701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2405862, whole genome shotgun sequence.
ACCESSION   DASYJA010000306 DASYJA010000000
VERSION     DASYJA010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2405862"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="VGO02_06720"
     CDS             complement(<1..1117)
                     /locus_tag="VGO02_06720"
                     /inference="COORDINATES: protein motif:HMM:NF025131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stimulus-sensing domain-containing protein"
                     /protein_id="HEV7477412.1"
                     /translation="MASDTDTATPEPGRRWFGLQGLGSRIAAAFGRRAAAPVPARTAE
                     SPSLLRRRRSASRRHSPLTRRILLLNLVPVALLTLGAVYLSDYEEELIDAELASLAVQ
                     GEMVAAGIGEVAVAGGETTVNRLDADAARQLLTRLVRPTGVRARLFSETGELLGDSAV
                     LSEAGRIHVMPLPPPEAATDVEAEFGDRLGDWLARQLSRADRFPAYVERAVPTVRDFP
                     EAASALRGFNASAVRVAPDGRLMLSAAVPVQRYKQVLGALMLTRDNRAIAASLREVRY
                     DMMVIAAAALGITVLLSLYLAGTITQPIVRLARAADEVRLARESRPEIPDLGKRGDEI
                     GDLNDALRSMTDALWQRINTIESFAADVAHELKNPLTS"
     gene            complement(1086..>1701)
                     /locus_tag="VGO02_06725"
     CDS             complement(1086..>1701)
                     /locus_tag="VGO02_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7477413.1"
                     /translation="QIKTYNDGASALEGLAQSPPDLGIFDIKMPRMDGMELLNRIRKT
                     HQFPVIFLTSKDEEIDEVLGLRMGADDFIRKPFSQRLLLERIRAVLRRADTSGPKGEG
                     QAERIVRGELVLDPSRHQCTWKTKEVHLTVTEFLLLKSLAERPGHVKNRDQLMDAAYG
                     ETIYVDDRTIDSHIKRVRRKFREVDGEFEQIETLYGIGYRYRDS"
BASE COUNT          247 a          566 c          621 g          267 t
ORIGIN      
        1 gcgaggtcag cggattcttg agctcgtgcg ccacgtcggc ggcgaagctc tcgatggtgt
       61 tgatgcgctg ccacagcgcg tcggtcatcg agcgcagcgc gtcgttgagg tcgccgatct
      121 cgtcgcctcg tttgccgaga tcgggaatct ccggccggct ctcgcgcgcc agccggacct
      181 cgtcggcggc gcgcgccagg cgcacgatcg gctgggtgat ggtgccggcg aggtagagcg
      241 acagcagcac cgtgatgccg agcgcggcag ctgcgatcac catcatgtcg taacggacct
      301 cgcgcagcga ggcggcgatg gcgcggttgt cgcgcgtcag catcagcgcg cccagcacct
      361 gcttgtagcg ttgcaccggc acggccgcgc tcagcatcag ccggccgtcg ggcgccacgc
      421 gcacggccga ggcgttgaag ccgcgcagcg cgctcgccgc ctcggggaag tcgcgcaccg
      481 tgggcacggc gcgctcgaca taggcgggaa agcggtcggc gcgcgaaagc tggcgggcga
      541 gccagtcgcc cagccggtcg ccgaactcgg cctcgacatc ggtggccgcc tcgggcggcg
      601 gcagcggcat cacgtggatg cggccggcct cgctcagcac tgccgaatcg cccagaagtt
      661 cgccggtttc gctgaacagc cgggcgcgca cgccggtcgg gcggaccagc cgggtcagca
      721 gctggcgcgc ggcgtcggca tcgaggcggt tgaccgtggt ctcgccgccc gccacggcga
      781 cctcgccgat gccggccgcc accatctcgc cctgcaccgc gagcgaggcg agctcggcgt
      841 cgatcagttc ttcctcgtag tcgctgagat agaccgcgcc cagggtcagc agcgccaccg
      901 gcacgaggtt gagcagcagg atgcggcggg tgagcggcga atggcgccgc gacgcgctgc
      961 ggcggcggcg cagcagcgag ggcgattcag cggttcgcgc gggcacaggc gcggcggcgc
     1021 ggcggccgaa cgccgcggcg atccgcgaac ccaggccctg cagtccgaac cagcgtcggc
     1081 cgggctcagg agtcgcggta tcggtatccg atgccataaa gcgtttcgat ctgctcgaac
     1141 tcgccatcaa cctcgcggaa cttgcggcgc acccgcttga tgtggctgtc gatggtgcgg
     1201 tcgtcgacgt agatcgtctc gccgtaggcc gcatccatga gctggtcgcg attcttcaca
     1261 tgccccggcc gctcggccag cgacttcagg agcaggaatt cagtgaccgt gaggtgcacc
     1321 tccttggttt tccaggtgca ctggtggcgg ctcgggtcga gcaccagctc gccgcgcacg
     1381 atgcgctcgg cctggccttc gcccttgggg ccgctggtgt cggcgcggcg cagcacggcg
     1441 cggatgcgct cgagcagcag gcgttgcgag aacggcttgc ggatgaagtc gtcggcgccc
     1501 atgcgcagtc ccagcacctc gtcgatctcc tcgtccttgg aggtgaggaa gatcaccggg
     1561 aactgatgcg tcttgcggat gcggttcagg agctccatgc cgtccatgcg cggcatcttg
     1621 atgtcgaaaa tgccgagatc gggcggtgac tgggcaagtc cttcgagggc cgaggcgcca
     1681 tcgttgtagg tcttgatctg g
//
LOCUS       DASYJA010000307         3388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2043155, whole genome shotgun sequence.
ACCESSION   DASYJA010000307 DASYJA010000000
VERSION     DASYJA010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2043155"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1628)
                     /locus_tag="VGO02_06730"
     CDS             complement(<1..1628)
                     /locus_tag="VGO02_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HEV7477414.1"
                     /translation="MQKRSKSRVKKALKKSASAARRSKVRAHAGKRPAKKPQHAKVAA
                     QARKLKAPKAVAAAKVPAAKLDPKAAAKQAKTPVKSAKQALELLEAKVNEVKAKGKGG
                     RGGRRSGKDAWLVPAELPKEDAETRRTRLKNLIKLGKERGFLTYSEINDHLPDDLVDA
                     EQIEAIISTFGDMGIQVYDQAPDQETLLIASDAAPVAQPDEDVEEQAEAALSTVDSEF
                     GRTTDPVRMYMREMGSVELLTREGEIEIAKRIEDGLRHMIQAISACPTTVQEILDLAT
                     KVEKDEMRIDEVVDGLIDPNAKEDFDPKKAAEPSAEEEEAQIEEEEGEDEEAAEAAQS
                     ASMLKLKGEALEKFARIRRLFEKLGKALENKGSKDKDYLRAREQILSELMTIRFTART
                     VERLSDSVRSLVDEIRRYERAIHDVSVEKAQMPRPHFIKVFAGTEGSKRWLTAEVNSE
                     QPWSEQMRRVEPSILELLGKITAVQQKIGIPIRDLKEINKQMSTGEAKARRAKREMTE
                     ANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAV"
     gene            complement(1766..>3388)
                     /gene="dnaG"
                     /locus_tag="VGO02_06735"
     CDS             complement(1766..>3388)
                     /gene="dnaG"
                     /locus_tag="VGO02_06735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEV7477415.1"
                     /translation="HGEKTPSFTVSPSKQFYHCFGCGVHGNAIGFLMAYAGMGYVDAI
                     KDLASSVGMQVPELQPRTPEEAVRRERETDLYGLMEQAMEFYRAELKKSPRAIEYLKG
                     RGLTGEIAARFRVGYAPDGWQGLKGVFQDYSHSSLVECGLVIEGDEGKRYDRFRDRVM
                     FPILSTRGAVIGFGGRVIGEGEPKYLNSPETPLFEKGREVYGLVQARDAIRTAGRVLV
                     VEGYMDVVALAQFNVGYAVATLGTATTPIHVTKLLKLADELVFSFDGDAAGRKAAWRA
                     LEVSLPLLVDHKPVRFLFLPEGDDPDTFIRKHGGEAFEKQVAGAKTLSEFLIDELRQG
                     ADLNSNEGRAKFLSAAKPYLQKMGGSQLPTLVAKEIASLARQPEEAPSLLARAPAPTN
                     RSVQQRANTAQQFDLEKRVLSLIFAKPALALEVDLQFFNQDYEETAAVAEICELVRRT
                     ENPEELSDAMLIELTRNSEHSRLIAEAQAVWLSFKLSPDQAEGEFRDAMTKLRTSLPK
                     DEKYLSTKVLKGTATAEERRAYEQRLADKRAH"
BASE COUNT          481 a         1114 c         1074 g          719 t
ORIGIN      
        1 accgccttca tcaggccgat gttgccttcc tggatcaggt cgaggaactg caggccgcgg
       61 ttggtgtact tcttcgcgat cgagatcacc aggcgcaggt tggcctcggt catctcgcgc
      121 ttggcgcggc gcgccttggc ctcgccggtc gacatctgct tgttgatctc cttcagatcc
      181 ctgatcggga tgccgatctt ctgctgcacg gccgtgatct tgcccagcag ctcgaggatc
      241 gagggctcga cgcgcctcat ctgctcgctc cagggctgct cggagttgac ctcggcggtg
      301 agccagcgct tggagccctc ggtgccggcg aacaccttga tgaagtgcgg gcgcggcatt
      361 tgcgccttct ccacgctcac gtcatgaatg gcgcgctcgt agcggcggat ctcgtcgacc
      421 aggctgcgca cgctgtcgga cagccgctcg acggtgcgcg cggtgaagcg gatggtcatc
      481 agctcgctca ggatctgctc gcgggcgcgc aggtagtcct tgtccttgga gcccttgttc
      541 tcgagcgcct tgccgagctt ctcgaacagc cgccggatgc gcgcgaactt ctccagcgcc
      601 tcgcctttca gcttcagcat gctcgccgac tgggcggctt cggcggcttc ctcgtcctcg
      661 ccctcttctt cctcgatctg cgcttcttcc tcctcggcgg agggctcggc cgccttcttc
      721 gggtcgaaat cttccttcgc gttgggatcg atcagcccat cgaccacttc gtcgatgcgc
      781 atctcgtcct tctcgacctt ggtggcgaga tccaggatct cctgcaccgt ggtcgggcag
      841 gcgctgatcg cctggatcat gtggcgcagg ccgtcctcga tgcgcttggc gatctcgatc
      901 tcgccctcgc gcgtcaagag ctctaccgag cccatctcgc gcatgtacat gcgcaccggg
      961 tcggtggtgc ggccgaattc ggaatcgacg gtcgagagcg cggcctcggc ctgctcctcc
     1021 acgtcctcgt cgggctgcgc caccggcgcc gcgtcggagg cgatgagcag cgtctcctgg
     1081 tcgggcgcct ggtcgtagac ctggatgccc atgtcgccga aggtggagat gatggcttcg
     1141 atctgctcgg cgtcgaccag gtcgtcgggc aggtgatcgt tgatctcgga gtacgtgagg
     1201 aagccgcgct ccttgccgag cttgatcagg ttcttgaggc gcgtgcgccg ggtctcggcg
     1261 tcttccttgg gcagctcggc cgggaccagc caggcgtcct tgccgctgcg gcggccgccg
     1321 cggccgcctt tgcctttggc cttgacctcg ttgaccttcg cttcgagcag ctcgagcgcc
     1381 tgcttcgcgc tcttgacggg tgttttcgcc tgcttggcgg cggccttggg gtcgagcttg
     1441 gccgcgggaa ctttcgcggc ggcgacggct ttcggcgcct tgagcttgcg cgcctgcgcc
     1501 gctactttcg cgtgctgcgg tttcttggcc ggccgcttgc cggcgtgcgc gcgaaccttg
     1561 ctgcgcctgg cggcactggc gctcttcttg agggccttct tcactctgga cttgctgcgc
     1621 ttctgcatct cttgcttccc ccgggattca gcgaaattcg cggacttggc gaaaagccca
     1681 agattgtacc aaaaggctga acattcaagg gctttgatct attccttccc cttgaatttc
     1741 aagcaagaaa tctgccaacg ccgcgctagt gggcgcgctt gtccgccaga cgctgttcat
     1801 acgctcgccg ttcctcggct gtcgcggttc cctttaagac cttggtcgaa agatactttt
     1861 catccttcgg cagactggtc cgcaacttcg tcatcgcgtc tctgaactcg ccttcggcct
     1921 gatccggact caacttgaaa gaaagccaga cggcctgcgc ttcggcaatc aggcggctgt
     1981 gttccgaatt gcgggtcaat tcgatcagca tggcgtctga gagttcctcg ggattttcgg
     2041 tgcgccttac cagctcgcaa atttccgcca ccgcggccgt ttcctcatag tcctgattga
     2101 agaactgcag gtccacctcg agcgccaaag cgggtttggc gaagatcagg ctgagcaccc
     2161 gcttttccag gtcgaattgc tgggcggtat tggctctttg ctggactgac cggttcgtcg
     2221 gggccggcgc gcgggcgagc aggctcggcg cctcctcggg ctggcgggcg agcgaggcga
     2281 tttccttcgc caccagggtc ggcagctggc tgccgcccat cttctgcagg tagggcttgg
     2341 cggcactcag gaatttggcg cggccctcgt tgctgttgag gtcggcgccc tggcgcagct
     2401 cgtcgatgag gaactccgac agcgtcttgg cgccggcgac ctgtttctcg aaggcttccc
     2461 cgccgtgctt gcggatgaat gtgtccgggt cgtcgccctc gggcagaaaa agaaagcgca
     2521 ccggcttgtg gtcgaccaga aggggcaggc tgacctccag cgcgcgccac gccgccttgc
     2581 ggccggccgc gtcgccgtca aacgagaaca ccagctcgtc ggccagcttg agcagcttgg
     2641 tgacgtggat cggcgtggtt gccgtgccca gcgtcgccac cgcatagccg acgttgaact
     2701 gggcgagcgc tacaacgtcc atgtagccct cgaccaccag gacgcggccg gcagtgcgga
     2761 tcgcgtcccg ggcctgcacc aggccgtaca cctcgcggcc cttctcgaag agcggcgtct
     2821 cgggcgaatt caggtatttg ggctcgccct cgccgatcac ccggccgcca aagccgatga
     2881 ccgcgccgcg ggtgctcagg attggaaaca tgacccggtc gcggaagcgg tcgtagcgct
     2941 tgccttcgtc gccttcgatc accaggccgc actcgaccaa cgaagaatga gaataatctt
     3001 gaaaaacccc tttcaggcct tgccagccat ccggggcata gccgacgcga aagcgcgccg
     3061 cgatctcgcc ggtcaggccc ctgcccttca ggtattcgat cgcgcgcggc gatttcttca
     3121 gctcggcgcg atagaactcc atcgcctgct ccatcaggcc gtagaggtcg gtctcgcgct
     3181 cccggcgcac cgcctcctcg ggcgtgcgcg gctggagctc aggcacctgc atgccgaccg
     3241 aggacgcgag gtccttgatg gcgtcgacgt agcccatgcc ggcgtaggcc atcaggaaac
     3301 ctatggcgtt gccgtgcacc ccgcagccga agcagtgata gaactgcttg gacgggctga
     3361 cggtgaacga gggggttttc tcgccgtg
//
LOCUS       DASYJA010000308         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2043417, whole genome shotgun sequence.
ACCESSION   DASYJA010000308 DASYJA010000000
VERSION     DASYJA010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2043417"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VGO02_06740"
     CDS             complement(<1..324)
                     /locus_tag="VGO02_06740"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011885973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEV7477416.1"
                     /translation="MAAAHRLPARIVAIGDRGLLAGCPQIEHVALAQPRVPGRLDPAN
                     ARYVLAILDRAVRGCLDGEYDAIVTAPVQKSVINDAGIAFTGHTEYLAAAAHAPHVVM
                     MLVGGG"
     gene            complement(387..1694)
                     /locus_tag="VGO02_06745"
     CDS             complement(387..1694)
                     /locus_tag="VGO02_06745"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV7477417.1"
                     /translation="MFTRSLVLLLCLLAWLPAALGQRAALVDRIVAVVNKEVITASEL
                     HEATASAERALGRRGTQLPPRELLERQMLERLILDKAQLQMAREMGIRVDELQLDRAV
                     QRVAEQNRLTLAEFRGALERDGVAFGAYREDLREQIILSRLREREVSDRIQVSDGEID
                     LLLEEMKAAPAERVEFNLAHVLVRIPEQASPERIEAARGRAEKVVLEARAGTDFGRLA
                     ASYSDAPDALQGGALGWRARDRLPDLFVSALAKMSPGEVSEVLRSPAGFHVVKLLERR
                     GAGGPAEAGVPVLQTRLRHILIRTSDSVSEDDARRRLLTLRERIVGGGADFAEMARVH
                     SDDGTAARGGELDWIYPGDTVPDFERGYVELKIGEVSEPVRTPFGYHLIQVLERRSAD
                     MSLERRRMQARQTLRERKMDDAYQEWLRLVRDQTYVELRLEEK"
     gene            complement(1694..>2130)
                     /locus_tag="VGO02_06750"
     CDS             complement(1694..>2130)
                     /locus_tag="VGO02_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477418.1"
                     /translation="VRYTPEPAKVINASYRFTRATDTLAGVNQIDVSGQWPVSAGWYA
                     VGRYNYSFLDGRLLDGLAGFERNAGCWIFRAVVQRVQAAQQVSSTGFFFQLEFNGVGQ
                     IGTSDVVQLLTRNVAGYSVTNPRDDALRPPSLQRKLPFEQVY"
BASE COUNT          303 a          764 c          745 g          318 t
ORIGIN      
        1 gccgccgccg accagcatca tgacgacgtg cggcgcgtgc gcggcggcag ccaggtactc
       61 ggtgtgcccg gtgaaggcga tgccggcgtc gttgatgacg cttttctgca ccggcgcggt
      121 gacgatcgcg tcgtactcgc cgtcgaggca gccgcgcacc gcgcggtcca ggatcgcgag
      181 cacatagcgg gcgttcgccg gatcgagcct gccgggcacg cgcggctgcg cgagcgcaac
      241 gtgctctatc tgcgggcagc cggcgagcag ccctcgatcg ccgatggcga cgatgcgcgc
      301 cggcagcctg tgcgcggcgg ccatgacgca gatctccggc ccgatgccgg cgggctctcc
      361 ggaagtcagc gcgatgaccg gaggctttat ttctcctcca gccgcagctc gacgtaggtc
      421 tggtcgcgta ccaggcgcag ccactcctgg tacgcgtcgt ccatcttgcg ctcgcgcagc
      481 gtctggcggg cctgcattct gcggcgctcg agggacatgt ccgccgagcg ccgttccagc
      541 acctggatca ggtgatagcc gaagggcgtg cgcaccggct cgctcacctc gccgatcttg
      601 agctcgacat agccgcgctc gaaatcgggc acggtgtcgc cggggtagat ccagtcgagc
      661 tcgccgccgc gcgcggcggt gccgtcgtcg gagtgcaccc tcgccatttc ggcgaaatcg
      721 gcgccgccgc cgacgatgcg ctcgcgcagc gtgagcaggc ggcggcgcgc atcgtcctcg
      781 gaaaccgagt cgctggtgcg gatcaggata tgccgcaggc gcgtctgcag caccggcacg
      841 ccggcctcgg cagggccgcc ggcgccgcgc cgctcgagca gcttgacgac gtggaagccc
      901 gccgggctgc gcagcacctc gctgacctcg cccgggctca tcttggcgag cgccgagacg
      961 aacaggtcgg gcagccggtc gcgcgcccgc cagccgaggg cgccgccttg cagcgcgtcg
     1021 ggcgcgtccg agtagctcgc ggccaggcgt ccgaagtccg tgccggcgcg cgcctcgagc
     1081 acgaccttct cggcgcggcc gcgcgccgcc tcgatgcgct cggggctagc ctgctcgggg
     1141 atgcgcacca ggacgtgcgc caggttgaac tcgacgcgct cggcgggcgc cgccttcatc
     1201 tcctcgagca ggaggtcgat ctcgccgtcg cttacctgga tgcggtcgct gacctctcgc
     1261 tcgcgcaggc gcgacaggat gatctgctcg cgcaggtcct cgcggtaggc gccgaaagcc
     1321 acgccgtcgc gctccagcgc gccgcggaac tcggccagcg tcaggcggtt ctgctcggcg
     1381 acgcgctgca ccgcgcgatc gagctgcagc tcgtcgaccc gaatccccat ctcgcgcgcc
     1441 atctgcagct gcgccttgtc gaggatcagc cgctcgagca tctggcgctc gagcagctcg
     1501 cgcggcggaa gctgcgtgcc gcgccggccc agggcgcgct ccgccgaggc ggtcgcctcg
     1561 tgcagctccg aggcggtgat gacttccttg ttcaccaccg ccacgatgcg atcgacgagc
     1621 gcggcgcgct gccccagggc ggcgggcagc caggcaagca ggcaaagcag cagcactagg
     1681 gagcgcgtga acatcaatag acctgctcga agggcagctt gcgctgcagg ctgggcggcc
     1741 gcagcgcgtc gtcgcgcggg ttggtcaccg aataacccgc gacgttgcgc gtcagcagct
     1801 gcactacatc gctggttccg atctggccca ctccgttgaa ttccagctgg aagaagaatc
     1861 ccgtcgagct cacttgttgc gccgcctgga cgcgctgcac tactgcccgg aagatccagc
     1921 agcccgcatt gcgctcgaaa cccgccaggc cgtcgagcag gcgtccgtcc aggaacgaat
     1981 aattgtaccg ccccacggcg taccagcccg cgctcaccgg ccactggccc gacacgtcga
     2041 tctggttcac cccggccagg gtgtcggtgg cgcgggtgaa gcggtagctc gcgttgatga
     2101 ccttggccgg ctcgggggtg tagcgcaccg
//
LOCUS       DASYJA010000309         6106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1316883, whole genome shotgun sequence.
ACCESSION   DASYJA010000309 DASYJA010000000
VERSION     DASYJA010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316883"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..677
                     /locus_tag="VGO02_06755"
     CDS             219..677
                     /locus_tag="VGO02_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477419.1"
                     /translation="MSVAVAALAGGTSHGFPGLYPVILWKTTLIAAGVASCAMVVATG
                     KATAIFPRTFAWLATVKFCIYCVWVALRDDFIIVVIDSGSALLIVATLHAIRGGPPWR
                     WMIGGVAVSVVAAGVQAMHLAPHPHFNHNDLYHVIQIAAMWLFYRGAKLI"
     gene            complement(674..1186)
                     /locus_tag="VGO02_06760"
     CDS             complement(674..1186)
                     /locus_tag="VGO02_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HEV7477420.1"
                     /translation="MSLSMYQASAPRFANTLKNLSALLDKTQAHCEAKKIDPLAITSS
                     RLFPDMFPFNRQIYIATDTAKGAVSRLAGIEIPKYEDTETTIPELKARLAKTIDFVMS
                     VPAAKIEGSAEKDISMKIAGKDTVLKGAQYLLGHAYPNFYFHCTTAYNILRHNGVEIG
                     KRDFLGTIVL"
     gene            1207..2454
                     /locus_tag="VGO02_06765"
     CDS             1207..2454
                     /locus_tag="VGO02_06765"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7477421.1"
                     /translation="MARLFYGWRMVGAATGMQFLQAALVNNAFGAYVAVLAEERGWSK
                     TALSGAAALQQMEAALLGPVLGWLLDRFGAQVFIRAGVFFLGGGLMLLSTVDSLAGFY
                     GAFVVIAMGGSLAGFFPLNVALIHWFERFRARALSSMSLGLAFGGIAAPLVAWSLQTY
                     GWRPTAFYSGVIAIVVGFPLALVMRNKPEDYGLKVDGAVELPFDKSSVNENKESTRDF
                     TAREALRTPAFWLLSLGHGFALFVVHTVLVHAITHLKEGLGYTLAAAALVMGLVTAFQ
                     IVGVMIGWWIGDRFDKRLIAAACMLMHAVGLLLLTYALAMPMLVGFAVLHGIAWGLRG
                     PFMQALRADYFGRASIGMILGLSYMIIIIGQMGGPLIAGAFADAHGNYQVGFTLLAVL
                     AGLGSIFFLLAKKPSRPLLESTA"
     gene            2451..2906
                     /locus_tag="VGO02_06770"
     CDS             2451..2906
                     /locus_tag="VGO02_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477422.1"
                     /translation="MSGGKFSGHSMRILIAVFLAATLLGCDTTVKKSELASVDYGPRP
                     QHFQATIRDYLKIRLTDPTAAIVEFRAGPQQLYQRETPLRKAQWGWGVCVWVNDKNKQ
                     GAFDGFYPMSVLIRNETIAHVNGGPDDDNLVGNRYAREHCEQLGAPFKK"
     gene            2903..3754
                     /locus_tag="VGO02_06775"
     CDS             2903..3754
                     /locus_tag="VGO02_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEV7477423.1"
                     /translation="MSTPPAAVATAIASSRLYKPAVALDFFKHGGKAERIPAGETIFA
                     EGRKGLIFRDKMYLLISGDVELMMRKKVITTLKAGEIFGELAAIDGAPRTASAVAKTN
                     CRLIGLDEKQFYAALRKKPGFALMLMSVMINRLRDTITRLLANGDMSEKEATKEQVTF
                     KPKLLEELVQGLADDPPVFFLQGKQIMQAGQTGLRMYAIVEGHVTVTIGGRVVERLGP
                     GGVFGEAALVDQSTRLASAVAQSDCSLLAISRQAFTALVKVSPEFADAILGSLAERMR
                     FLTARVQ"
     gene            3775..4074
                     /locus_tag="VGO02_06780"
     CDS             3775..4074
                     /locus_tag="VGO02_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477424.1"
                     /translation="MSAPSKETPKAPQHSDPMAELAEKIFVALAGRIYGNLAGGDVKK
                     PDPKALATYSYKLAEAFEQASKETDRARASIEAANKAKVRLDEVDLTSVFADVKK"
     gene            complement(4078..5151)
                     /locus_tag="VGO02_06785"
     CDS             complement(4078..5151)
                     /locus_tag="VGO02_06785"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEV7477425.1"
                     /translation="MEERRESRREEGLRFARRMYLPRAAGLALGAICIAGVLWTNGAS
                     PAAWAGLALSCLAWPHLAYRAARHARDPYRAELRNLSVDSAFGGAWIAAMQFNLLPSV
                     VIFVMLCMDKLSVGGARFLARCLAAQAVAAAAVIAVFGLQFEPATTMLEIVCSLPLLV
                     VYPFAVGTVTYRLTRTVRSQNRMLAELSRTDALSRLPNRGHWEEEVAAAAQRCRSSGQ
                     EAALMMIDIDYFKAINDRYGHPAGDEVIRAVAAILRDSLRGNDIAGRYGGEEFGVLLP
                     ETPAAGATATAERLRARVEAADLERSLGIRGTVSVGIAFFSMRDAGHAQWIARADRAL
                     YMAKGAGRNRVVRDDGADPAPAA"
     gene            complement(5154..5378)
                     /locus_tag="VGO02_06790"
     CDS             complement(5154..5378)
                     /locus_tag="VGO02_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477426.1"
                     /translation="MNPESVLEKTTKGAEEIETRKHKLDAKLRPVLIAVNGKLTAGEL
                     AAQFAQLGDTAPLLDDLMKQGFVRVLALGG"
     gene            5505..5762
                     /locus_tag="VGO02_06795"
     CDS             5505..5762
                     /locus_tag="VGO02_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477427.1"
                     /translation="MELDFKALAAGLATAAVFAFAAHAHPGDKFRSLDRNGDGFLSKS
                     EVSGIEGYEAAFNEADEDHDGRLSPAEFVKALAILDRQAVK"
     gene            complement(5805..>6106)
                     /locus_tag="VGO02_06800"
     CDS             complement(5805..>6106)
                     /locus_tag="VGO02_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477428.1"
                     /translation="VPHTKGKPMEHQQARPSTPEEVPSTQTEAAMKSMRRSVDHAIAS
                     ASDRTREIVQYADRRVQASPWTAVGISFGAGMIFGVLVAMAAASQQPRGFLDRWR"
BASE COUNT          966 a         2060 c         2047 g         1033 t
ORIGIN      
        1 ttcccgcagt tccgcgactt cctgctgctg aagaggaagt acgacaagga agagctcttc
       61 cagagcgact ggtaccggca ctacaagagg atgtacaacc tctgagcatc gccgagccgg
      121 cgacgctgct caccgactac ctgcttgctg ctgtttgcct ctttcttgct tttcgcctgg
      181 taaaggacaa gaggtcgggc tggtgggcga cggctttcgt gtccgtggcg gttgccgcgc
      241 tggcaggtgg aacgtcgcat ggctttcccg gcctgtatcc ggtcattctc tggaaaacaa
      301 cgctcatcgc cgccggtgtc gcgagttgcg cgatggtcgt tgcgaccggg aaagccacgg
      361 ccatctttcc gcgaactttc gcatggctgg caaccgtcaa attctgcatc tactgcgtct
      421 gggtggcgtt gcgcgacgac ttcatcatag tcgtgatcga cagcggcagc gcgctgctga
      481 tcgtcgcgac gctgcacgcc atccgcggcg ggccgccgtg gcgctggatg atcggcggcg
      541 tggcggtatc ggtcgtcgcg gccggggtgc aggcgatgca tctcgcgccg cacccgcact
      601 tcaaccacaa cgacctctac cacgtgatcc agatcgccgc gatgtggctg ttctaccgcg
      661 gcgcgaagct gatctagagg acgatcgtcc ccaggaagtc gcgcttgccg atctcgacgc
      721 cgttgtggcg caggatgttg taggcggtag tgcagtggaa atagaagttc ggataggcgt
      781 ggccgagcag gtactgcgcg cccttcagca cggtgtcctt gccggcgatc ttcatcgaga
      841 tgtccttctc ggccgagccc tcgatcttcg ccgccggcac gctcatcacg aagtcgatgg
      901 tcttcgcgag gcgcgccttg agctccggga tggtggtctc ggtgtcctcg tacttcggga
      961 tctcgatgcc ggcgaggcgc gagacggcgc ccttggcggt atccgtggcg atgtagatct
     1021 gccggttgaa cgggaacatg tcggggaaca gccgggacga ggtgatcgcc agcggatcga
     1081 tcttcttcgc ttcgcagtgc gcctgcgtct tgtcgagcag cgcggagagg tttttgaggg
     1141 tgtttgcgaa acgcggcgcg ctcgcctgat acatggacag cgacatgagc ttactctctg
     1201 gagttgatgg cacgcctatt ctacggctgg cgcatggtgg gcgcggcgac cggcatgcag
     1261 ttcctgcagg cggcgctggt gaacaacgcc ttcggcgcct acgtcgcggt gctcgccgag
     1321 gagcgcggct ggtcgaagac cgcgctctcc ggcgcggcgg cgctgcagca gatggaggcg
     1381 gcgctgctcg gcccggtgct cggctggctg ctcgatcgct tcggcgcgca ggtcttcatc
     1441 cgcgccggcg tcttcttcct cggcggcggc ctgatgctgc tcagcacggt ggactcgctg
     1501 gcgggcttct acggcgcgtt cgtggtcatc gccatgggcg gcagcctggc ggggttcttc
     1561 ccgctcaacg tcgccctgat ccactggttc gagcgcttcc gggcgcgcgc tctgtcctcg
     1621 atgtcgctcg gcctcgcctt cggcggcatc gccgcgccgc tcgtcgcctg gtcgctgcag
     1681 acctacggct ggcgcccgac cgcgttctac tcgggcgtga tcgccatcgt ggtcggcttc
     1741 ccgctcgcgc tggtgatgcg caacaagccg gaagactacg gattgaaggt ggatggcgcg
     1801 gtcgaattgc ctttcgataa aagttctgtg aatgaaaaca aagaatcgac tcgcgacttc
     1861 accgcgcgcg aggcgctgcg cacgccggcc ttctggctgc tctcgctcgg ccacggcttc
     1921 gccctgttcg tcgtgcacac ggtgctggtg cacgccatca cgcacctgaa ggagggcctg
     1981 ggctacacgc tggccgcggc ggcgctggtg atgggcctgg tcaccgcgtt ccagatcgtc
     2041 ggcgtgatga tcggctggtg gatcggcgac cgtttcgaca agcgcctgat cgcggcggcc
     2101 tgcatgctga tgcacgcggt ggggctgctg ctgctcacct atgcgctcgc catgccgatg
     2161 ctggtcggct tcgcggtgct gcacggcatc gcctggggcc tgcgcggccc gttcatgcag
     2221 gcgctgcgcg ccgattactt cggccgcgcc tcgatcggca tgatcctcgg cctgtcctac
     2281 atgatcatca tcatcggcca gatgggcggg ccgctgatcg ccggcgcctt cgccgatgcg
     2341 cacggcaact accaggtcgg cttcaccctg ctcgccgtcc tcgccggcct gggctcgatc
     2401 ttcttcctgc tggcgaaaaa accctcgcgc ccgctgttag aatctacggc atgagtgggg
     2461 ggaagttttc ggggcactcg atgagaattt tgattgcagt ctttctggcc gcaaccctcc
     2521 tgggttgcga caccaccgtg aagaagtccg agctggcgtc ggtcgattac gggccgcggc
     2581 cgcagcattt ccaggcgacg atccgcgact acctgaagat ccgcctcacc gacccgacgg
     2641 ccgcgatcgt cgagttccgc gccggcccgc agcagctcta ccagcgcgag acgccgctgc
     2701 gcaaggcgca atggggctgg ggcgtgtgcg tgtgggtcaa cgacaagaac aagcagggcg
     2761 ccttcgacgg gttctacccg atgagcgtgc tgatccgcaa cgagaccatc gcgcacgtca
     2821 acggcggtcc cgacgacgac aacctggtcg gcaaccgcta tgcgcgcgag cactgcgagc
     2881 agctcggcgc gccgttcaag aaatgagcac gccgcccgcg gctgtcgcca ccgccatcgc
     2941 ttccagccgg ctgtacaagc cggcggtggc gctcgacttc ttcaagcacg gcggcaaggc
     3001 cgagcgcatc ccggccggcg agaccatctt cgccgagggc aggaaggggc tcatcttccg
     3061 cgacaagatg tatctcctga tcagcggcga cgtcgagctg atgatgagga agaaggtcat
     3121 caccaccctc aaggcgggcg agatcttcgg cgagctggcg gcgatcgacg gcgcgccgcg
     3181 caccgcgagc gcggtggcca agaccaactg caggctgatc ggcctggacg agaagcagtt
     3241 ctacgccgcg ctgcgcaaga agccggggtt cgcgctgatg ctgatgagcg tgatgatcaa
     3301 ccggctgcgc gacaccatca cgcgcctgtt ggcgaacggc gacatgtccg agaaggaagc
     3361 caccaaggag caggtgacct tcaagccgaa gctgctcgag gagctggtgc agggcctggc
     3421 cgacgacccg ccggtgttct tcctgcaggg caagcagatc atgcaggccg ggcagacggg
     3481 cctgcgcatg tacgcgatcg tcgaggggca cgtcacggtg accatcggcg ggcgcgtggt
     3541 cgagcgcctc ggcccgggcg gggtgttcgg cgaggcggcg ctggtcgacc agtcgacgcg
     3601 gctcgccagc gcagtcgcgc agagcgattg ctcgctgctt gccatcagcc ggcaggcgtt
     3661 caccgcattg gtgaaggtca gcccggagtt cgccgacgcc atcctcggct cgctcgccga
     3721 gcgcatgcgc ttcctgactg cgcgggtaca gtaaccggta ttacactgtc ggctatgagc
     3781 gcaccctcca aggaaacccc gaaagcacct cagcacagcg acccgatggc cgagctggcg
     3841 gagaagatct tcgtcgcgct cgccgggcgc atctacggca acctcgccgg cggcgacgtg
     3901 aagaagcccg accccaaggc gctcgccacc tacagctaca agctcgccga ggccttcgag
     3961 caggcctcga aggaaaccga ccgcgccagg gcttccatcg aggcggcgaa caaagccaag
     4021 gtccggctcg acgaagtcga cctgaccagc gtcttcgccg acgtcaagaa gtagccctca
     4081 ggctgccggc gcggggtccg cgccgtcgtc gcgcaccacg cggttgcgcc cggccccctt
     4141 ggccatgtac agcgcccgat cggcgcgcgc gatccattgc gcgtggcccg cgtcgcgcat
     4201 gctgaagaag gcgattccca cgctcaccgt gccgcggatg ccgagactgc gctcgaggtc
     4261 cgccgcctcg acgcgcgcgc gcaggcgctc cgcggtggcc gtcgcgccgg ccgccggcgt
     4321 ctccggcagc agcacgccga actcctcgcc gccgtagcgc ccggcgatgt cgttgccgcg
     4381 cagcgagtcg cgcaggatcg ccgcgaccgc gcggatcacc tcgtcgcccg ccgggtgccc
     4441 atagcggtcg ttgatcgcct tgaagtagtc gatgtcgatc atcatcagcg ccgcctcctg
     4501 cccgctcgag cggcagcgct gcgccgccgc ggccacctcc tcctcccagt ggccgcggtt
     4561 gggcaggcgc gagagcgcat cggtgcgcga gagctccgcg agcatgcggt tctggctgcg
     4621 cacggtacgc gtcagccggt aggtgaccgt gcccacggcg aacgggtaga cgacgagcag
     4681 cggcaggctg cagacgatct cgagcatcgt ggtggcgggc tcgaattgca ggccgaagac
     4741 tgcgatcacc gccgccgccg cgacggcctg cgcggcgagg cagcgcgcga gaaacctcgc
     4801 gccgccgacg ctcagcttgt ccatgcacag catgacgaag atcaccacgc ttggcagcag
     4861 gttgaactgc atcgccgcga tccacgcgcc gccgaaggcc gagtcgacgc taaggttgcg
     4921 cagctcggca cggtacgggt cgcgggcgtg gcgcgccgcc cggtaggcaa ggtgcggcca
     4981 ggcgaggcag gagagcgcga gccccgccca cgctgccggc gaggcgccgt tggtccacag
     5041 cacgccggcg atgcagatcg cgccaagcgc gagccccgcc gcgcggggca ggtacatgcg
     5101 ccgggcgaaa cgcagccctt cctcgcgccg gctttcccgc ctctcctcca ctgtcagccg
     5161 ccgagggcga gcacgcgcac gaagccctgc ttcatcagat cgtcgagcag cggcgctgtg
     5221 tcgccgagct gcgcgaactg ggccgccagc tcgcccgccg tcagcttgcc gttgaccgcg
     5281 atcaggacgg ggcgcagctt ggcatcgagc ttgtgcttgc gcgtctcgat ctcctccgcc
     5341 cctttcgtcg tcttctccag gacgctctcg ggattcatcg gtaacctcct tgccgtaaat
     5401 cctacctccc aatcgtcccc cgcgggcgac gaacctgacg agaacccaac ccagggaccg
     5461 cgctctaacc gggagcaggg aatcaagaaa ggaaggagtg aacaatggaa ctggacttca
     5521 aagcgctcgc cgccggcctc gccacggccg ccgtattcgc attcgccgcg cacgcccacc
     5581 cgggcgacaa gttccgcagc ctggaccgca acggcgacgg cttcctgtcg aagagcgagg
     5641 tgagcgggat cgaaggctac gaggccgcgt tcaacgaggc cgacgaagat cacgacggca
     5701 ggctcagccc cgccgagttc gtcaaggcgc tggcgatcct cgaccgccag gccgtgaagt
     5761 aaggactgca cgccgggccc gttcaaggcc cagcggcgcg ccgcctagcg ccagcggtcc
     5821 aagaagccgc gcggctgctg cgaggccgcg gccatggcga ccagcacgcc gaagatcatg
     5881 ccggcgccga agctgatgcc gaccgcggtc cacgggctcg cctggacccg cctgtcggcg
     5941 tattgcacga tctcgcgcgt acggtccgaa gcgctggcga tcgcgtgatc gacggacctg
     6001 cgcatgctct tcatcgccgc ctcggtctgc gtcgatggga cttcctccgg ggtgctcggc
     6061 ctcgcctgct ggtgttccat gggcttccct ttcgtgtgag gtaccc
//
LOCUS       DASYJA010000310        16629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1408085, whole genome shotgun sequence.
ACCESSION   DASYJA010000310 DASYJA010000000
VERSION     DASYJA010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16629
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408085"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VGO02_06805"
     CDS             <1..490
                     /locus_tag="VGO02_06805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEV7477429.1"
                     /translation="PRVEEPRRPVVKRVPSPKAPAPKALPEPAPEAAPAEPAPAAPAE
                     TGTAPAASAAPPPADAIDADELARFRMRVIEAAMQFKRYPRAAQENNWVGRVDVRVTF
                     GADGRRASIVVVRSSGHEILDKQALDTITKALVPVPPALRGKGFALEIPVTYNLNDNP
                     SG"
     gene            complement(482..1570)
                     /locus_tag="VGO02_06810"
     CDS             complement(482..1570)
                     /locus_tag="VGO02_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7477430.1"
                     /translation="MRITIISPVNSPSPLLEVRELSHAYGTHQVVRDLSFSLPRGAIG
                     CLLGPSGCGKTTVLRCIAGFEAAQRGTIHIGGREVSAPGAMLAPEKRRIGMVFQDYAL
                     FPHLSVRGNIVFGLRGPGAERNARVAELAALVGLTAALDKYPHEISGGQQQRVALARA
                     LAPRPDLLLLDEPFSNLDTELRERLSAEVREIIKASGATAVLVTHDQQEAFAVADEIG
                     VLHQGRIQQWDTAYNLYHRPANRFVADFVGQGVFLPARALNGQEIEIELGVLKGTTPH
                     NGHGFEVLLRPDDVVHDDDASTKAEVVHKAFRGAEILYTLRLGSGRKVLALVPSHHNH
                     AIGERIGIRLDVDHVVAFAAAEGGAPLA"
     gene            1573..3210
                     /locus_tag="VGO02_06815"
     CDS             1573..3210
                     /locus_tag="VGO02_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV7477431.1"
                     /translation="MLEWQILKVQTLRVISIAVALLLALPVASVLGSVLAGGGETWSH
                     LAATGLARYVGNTLLLLVGVACGVISIGVLCAWIVTVYRFPGRDFLEWALMLPLAMPA
                     YVMAYAYTDWLQFAGPVQTSLRAATGWQAREYWFPEIRSLGGAIAMLSLALYPYVYLI
                     ARTAFLELSRSAIEAGRLAGLSAWGAFWHVAVPLARPAIAAGAALALMETLADFGTVS
                     YFAVEVFTTGIFKAWLSMGDTVAAAQLSSCLLALVVLVVALERAGRGRAAYAAPGRRP
                     APPQRLHGGASLAALAACAAPLALGFALPAGLLGRLAWDDAGLLVSARLATLVGNSFA
                     VAGLTAALAVVLATLMAYAARIERSGWVNVANRAASLGYAVPGAVIAIGVLVPLGRFD
                     NAAAAWIEHAFGVKMGLLLTGSVAALIYAYLVRLLAVALQTTEAGLAKITPSMEDAAR
                     SLGASPAGALARVHVPLLAPSIAAAALLVFVDVLKELPATFALRPFNFDTLAIEAYNL
                     AKDERLAEAAAPSLVIVAVGLIPVLFVTKRYFQATRS"
     gene            complement(3186..4190)
                     /locus_tag="VGO02_06820"
     CDS             complement(3186..4190)
                     /locus_tag="VGO02_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV7477432.1"
                     /translation="MLAAAALFAAIPAAAQDKVLNLYSSRHYQTDEALYTGFTKATGI
                     KINRIEGGEDALIERVRNEGERSPADVLITVDAGRLWRAEQLGLFQPTRSAMLESRIP
                     ASLREPSGLWFGFSTRARLIAYNKAKVKAGEIQTYEELADPKWKGRVCMRSSTNVYNL
                     SLMGALIDHLGEEKAQAWAQGVRTNLAVDPKGGDTDQLKAVAAGECDVTISNQYYYAR
                     LLRSQKPDEQQIGERLGVVFPNQKTWGTHVNISGAGVLKNAPNREAAVRFLEYLASDE
                     AQRYFADGNNEWPAVPGVRIDNPVLSRLGDFKRDALNVAVLGKNQPSSQKIYDRVAWK
                     "
     gene            complement(4252..4722)
                     /locus_tag="VGO02_06825"
     CDS             complement(4252..4722)
                     /locus_tag="VGO02_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEV7477433.1"
                     /translation="MKQLLLLCGWLCLFPLPALATTAQGASSREGPLAYALRALGARY
                     RYGGSSPETGFDCSGLIAHAFEQAWGLPLPHNAQAQSRLGKPVKRPNLAPGDLVFYNT
                     RDQPYSHVGMYIGNGRFIHAPKPGARVRIERFDTPYWQVRFNGARRLDIDNYSH"
     gene            4779..5567
                     /locus_tag="VGO02_06830"
     CDS             4779..5567
                     /locus_tag="VGO02_06830"
                     /inference="COORDINATES: protein motif:HMM:NF015891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEV7477434.1"
                     /translation="MPLIRSGLAITFLCAAQAWAQGALEIIPLKHRTVDQVLPVLRPL
                     LDPGATLSGQSGQLFVRTSPENLAELRQALEAIDRPSRRLQILVRFDDALESSSRDVA
                     ASGTISNRGSRVELRAQEAQAGATERIDQRVQVVEGGTAWITTGQSRTLPQRQLIRTP
                     GGVVSQETFVVQEATTGFAVSPRLSGSTVHLDISPQREALAGGGAVQSQRIATSASGR
                     LGEWFEIGGIAAASARDERGVASAARTTGGESRRIWVKVEELRN"
     gene            5654..7471
                     /locus_tag="VGO02_06835"
     CDS             5654..7471
                     /locus_tag="VGO02_06835"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEV7477435.1"
                     /translation="MAAAIEEAIGRNGVLVAEAGTGTGKTFAYLVPALLSGGKVIVST
                     GTKTLQDQLFDRDLPAVREALASGASVALLKGRSNYVCLYRLGRARGEARLETREQVA
                     QLARIEQFSRASVTGERADLADVPEDAAVWAHATSTRENCLGQECPDYRDCFVMRARR
                     NALAADVVVVNHHLFFADVVLRDEGVAELLPACNTLVFDEAHQLPETARMFFGETVSS
                     AQLVELSRDLRVELRAAGGASPELDALAGRLEKAARDLRLALGEAGTRLAWSQALTLP
                     GFPDSLKRLTQALRDVQAPLAAQAERSEGLDSCARRAGAASALVLRMHEESSGEVRWV
                     EVFGQAAQLHLTPLSPAELFRKQMSDHPRAWIFTSATLAVGNDFSHFTRELGVAEAET
                     RRWASPFDFARQALLYLPRGLPADPNAPGFTEAVVEAALPVLRASGGRAFLLFTTLRA
                     LRKAHELLRDRIEFPLLVQGTGSRSELLERFRRLGNAVLLGSQSFWEGVDVRGEALSV
                     VVIDKLPFAPPDDPVLAARIDGLKAREGNPFTELQLPRAVLQLKQGAGRLIRDETDRG
                     VLMLCDPRLVSKSYGRGIIRSLPPMKLTRELPEVEGFFG"
     gene            complement(7468..8400)
                     /gene="denD"
                     /locus_tag="VGO02_06840"
     CDS             complement(7468..8400)
                     /gene="denD"
                     /locus_tag="VGO02_06840"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HEV7477436.1"
                     /translation="MSKRILVIGAAGMIGRKLTARLAREGMGELILHDVVPFEGSSMV
                     SDLSVPGEAEKLVAKRPDLIFHLAAIVSGEAEADFEKGYRINLDGTRRLFEAVRKIGD
                     GYKPKLVFTSSIAVFGAPFPEAIGDEFLNAPLTSYGTQKAIGELLLSDYSRRGFFDGI
                     GIRLPTICVRPGKPNKAASGFFSGILREPLAGQEAVLPVPDTVRHWFASPRAAIEFLI
                     HASKLNTELLGTRRNLSMPGLSATIAEEIDSLRRVGGEKAVRLIRREPDPTIMRIVEG
                     WPRNFDAKRSLALGFRADPSFDDIVRYHVADELS"
     gene            complement(8397..9212)
                     /locus_tag="VGO02_06845"
     CDS             complement(8397..9212)
                     /locus_tag="VGO02_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEV7477437.1"
                     /translation="MQRSLLSAALLVAVLLAGGCSLLSSSPDDETIGWSAQRLYGEAK
                     DAQAGRDWPKAVKYLEKLEARYPYGRFAQQAQLEIAYCFWKDDERASAIGAVDRFIKL
                     YPNHPSIDYAWYLKGMINFGLQQNAIAQIATPDVTDRDPKASREAFDAFKEVVTRYPE
                     SKYAEDAEARMYFLVNALASHEVHVARYYMKRGAYLAAANRAQYAIRFYPNAPALEEA
                     VFVLVKAYDALGMDELRDGADRVMRANFPNSRYLKAGGDRRDAPWWRIWDPDW"
     gene            9211..10155
                     /locus_tag="VGO02_06850"
     CDS             9211..10155
                     /locus_tag="VGO02_06850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV7477438.1"
                     /translation="MKGDNYSDDWDAGFAAPMSETVPAGLGGLRLDQVLVRLFPQYSR
                     NRLQAWLKSGHIRVNGASLEAKHPVAGGERIVLEPPPPPAVVPQAQRMPLKIVHEDAS
                     LIVIDKPAGLVVHPGAGQPDGTLLNGLLAHSPALAGVPRAGIVHRLDKDTSGLLVVAK
                     TVVAQASLVKQLADRSMRRVYLAVVQGDPPASGVIDAPVGRDVRARVRMAVRHGGKDA
                     RTSYRVLERFGHAALVECRLETGRTHQIRVHFQHIRHPLVGDTVYRRGTRHGLEFPRQ
                     ALHAAELSLKHPQTGKLMTWQAPLPADMKRLLKRLEDD"
     gene            10148..10798
                     /gene="pgeF"
                     /locus_tag="VGO02_06855"
     CDS             10148..10798
                     /gene="pgeF"
                     /locus_tag="VGO02_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEV7477439.1"
                     /translation="MIEPDWPAHARVRALMTTRPMGDMAGEGRRSLEGLLPGEPAWLT
                     QVHGVSVTEIDSPSRSLKADAAVSRKAGNVCVVGAADCMPLLLSDKSGSVVAAAHAGW
                     RGLSAGVIEAAVRAMGVPPRHVLAWLGPAIGPRAYEIGEYVRAAFASYPDAFSAPTRP
                     GHWLLDLYAVARERLGDMGVTQIHGGGFCTYTDAARFYSYRRDGTKARMAALIWLT"
     gene            10812..11570
                     /locus_tag="VGO02_06860"
     CDS             10812..11570
                     /locus_tag="VGO02_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEV7477440.1"
                     /translation="MTLGWILAASFAGGTLSAALAAFSLYLRASWIPMLVSFAIGTLL
                     GAAFLEVIPAAFDQGDAHEAASAILGGIFGFFVLEKLMLWRHCHTEDCEAHDQHATHD
                     HGRSGALIIVGDTIHNFVDGVLIAAAFLQGIEIGIVAAVAIIAHEIPQEVGDFLILLH
                     SGYTRARALAMNLLSSLATLVGGLLGYYSLQAFEGLEPTLLGIVGASMIYVAVADLIP
                     GLHRRPQLRDGATQTLLIALGIGCIVLVRVMVAH"
     gene            11597..13306
                     /gene="phaC"
                     /locus_tag="VGO02_06865"
     CDS             11597..13306
                     /gene="phaC"
                     /locus_tag="VGO02_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEV7477441.1"
                     /translation="MQHAGSPEALLAAIAQSGDEVTQGWMRLLSSAAGDAGWLADLQR
                     NSARFGSVQAAYFEKQTRLWTGLLSGSDEPVAAPRAGDRRFSGKEWRDNPYYNYLRQS
                     YLLAASYIEELVEGCELKPVAKERLRFAARQWIDAISPANFAATNPAALSQAIATQGE
                     SLTRGLANLLGDVQKKRISQSDEAGFEVGRNIGVTPGSVVFENELIQLIQYRAATEKV
                     AARPLVIVPPCINKYYILDLQAENSFVAHAIRAGHTVFMVSWRNVGPEQGHLTWDDYL
                     EKGVFSALRAAREIAGSEQVNALGFCVGGTLLGAALSVLSRKNQNPVASATFLATMLD
                     FSDTGQIGLFVDESSVAAREAAIGRGGVFPGSDLAFVFSSLRANDLIWPYVVNNYLLG
                     EAPAAFDLLYWNADSTNLPGPMYCYYLRNTYLENNLRKPGALLNCGVPVDLGEVRLPL
                     FVLATCEDHIVPWRSAYRTIGLLGGTDKTFVLGASGHIAGVVNPPAKKRRSYWTGAPY
                     PKDPNEWLDKAKEIPGSWWPVWSEWLSRHKGGEREAPRAAGNVQYKAVEAAPGRYVKQ
                     RLH"
     gene            13316..14494
                     /locus_tag="VGO02_06870"
     CDS             13316..14494
                     /locus_tag="VGO02_06870"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003267761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEV7477442.1"
                     /translation="MEDIVIVGAARTAIGKFGGSLAKTPASDLGALIIRKVLERAGVA
                     PEQVSEVIMGHVLTAGVGQNPARQAAMKAGLPNMIPASTINVVCGSGLKAAMLGAQAI
                     ANGDSDIVVAGGQESMSLSPHVLNGSRDGFRMGDAKLVDSMIVDGLWDVYNQYHMGVT
                     AENVAKEYAVTREEQDEFAVASQHKTEAAQKAGRFKDEIVTVEIASRKGPVMFTDDEF
                     PRHGTTLEAMKGLKPAFDKQGTVTAGNASGINDGAAAIVLCSLKKSKELGLKPIARIK
                     AFASAGVDPKYMGMGPVPASKRCLARAGWQPKDLDLMEINEAFAAQAIAVNRQMGWDT
                     KKVNVNGGAIALGHPIGASGARILVTLLHEMQKRDAKKGLASLCIGGGMGVALAVERT
                     "
     gene            14494..15282
                     /locus_tag="VGO02_06875"
     CDS             14494..15282
                     /locus_tag="VGO02_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HEV7477443.1"
                     /translation="MGTQSEIVADPKTGQPVATRIAFVSGGMGGIGTAICRRLGRAGH
                     TVVAGCLPEYEKKAEWLAQMRGEGIRVHAAEGDVSEFDSCAEMFYHIRSVVGPVEILV
                     NNAGITRDSVLKRMTEQDWMAVINTNLNSVFNVTRQVIDGMSERGWGRIINISSVNAL
                     KGQFGQTNYSAAKAGMAGFSKALAQEVVKKGVTVNTVSPGYVETDMVMAIRKEILEQI
                     VASIPMGRLAKPEEIAAVVAFLASEEAGYITGANISVNGGMHMM"
     gene            15307..16053
                     /gene="phbB"
                     /locus_tag="VGO02_06880"
     CDS             15307..16053
                     /gene="phbB"
                     /locus_tag="VGO02_06880"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEV7477444.1"
                     /translation="MAGKKVAVITGGMGGLGESITTKMADAGYRVVVTYSPSNTKYRA
                     WLEEMKGRGYSFSAFPVDVVDFDDCARKCAQIQGECGAIDILVNNAGITRDMTFKKMT
                     KADWDAVMRTNLDSCFNMTKQVMDGMVERTWGRIINVSSVNGQKGAFGQTNYSAAKAG
                     IHGFTKALALEVARKGVTINTISPGYIGTKMVTAIPKEILDSKILPQIPTGRLGKPDE
                     VAGLIIYLCSDEAAFVTGANISINGGQHMY"
     gene            16079..>16629
                     /gene="phaR"
                     /locus_tag="VGO02_06885"
     CDS             16079..>16629
                     /gene="phaR"
                     /locus_tag="VGO02_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEV7477445.1"
                     /translation="MPETKSENHLRLIKKYPNRRLYDTKTSSYITLADVKQMVLKQED
                     FQVVDAKSGEELTRQILLQIILEEEQGGLPMFSSDLLSQLIRFYGNAMQGLMGTYLEK
                     NIRAFHDIQKTLQEQSARMYGGDTTRGTQDLWAQFMNLQGPAMQTLMQAYVEQSQKMF
                     AQFQDQVQNQARNMFTGFTFPGFT"
BASE COUNT         2642 a         5541 c         5753 g         2693 t
ORIGIN      
        1 gccgcgtgtc gaggagcctc ggcggcctgt ggtgaagcgg gtaccttcgc cgaaagcacc
       61 cgcgcccaaa gcgctcccgg aacctgcgcc ggaagcggca cccgccgagc cggcgcccgc
      121 cgcaccggcc gagaccggca ccgcgccggc ggcgagcgcg gcgccgccac cagccgatgc
      181 catcgatgcc gacgagctcg ccagattccg catgcgggtc atcgaggcgg cgatgcagtt
      241 caagcgctat ccgcgcgcgg cgcaggagaa caactgggtg ggaagggtcg acgtgcgcgt
      301 gactttcggc gccgacgggc ggcgcgcctc gatcgtcgtc gtgcgcagct ccgggcacga
      361 gatactcgac aagcaggcac tcgacacgat caccaaggcg ctcgtgccgg tgccgccggc
      421 gctgcgcggc aaggggttcg cgctcgagat cccggtgacc tacaacctga acgacaatcc
      481 ttcaggctag cggcgcgccg ccctctgccg cggcgaaggc gacgacgtgg tcgacatcca
      541 ggcggatgcc gatgcgctcg ccgatggcgt ggttgtggtg gctgggcacc agcgcgagca
      601 ctttgcggcc gctccccagg cgcagcgtgt agaggatctc ggcgccgcgg aacgccttgt
      661 gcaccacctc ggccttggtc gacgcgtcgt cgtcgtgcac gacatcgtct ggcctgagca
      721 gcacttcgaa gccgtggccg ttgtgcggtg ttgttccttt caacacgccc aactcgatct
      781 cgatctcctg cccgttgagc gcccgggcgg gcaggaacac gccctggccg acgaagtccg
      841 cgacaaagcg gttcgccggg cggtgataca ggttgtacgc ggtgtcccac tgctggatgc
      901 ggccctggtg cagcacgccg atctcgtcgg ccaccgcgaa cgcctcctgc tggtcgtgcg
      961 tcaccaggac cgccgtggcg ccgcttgcct tgatgatctc gcgcacctcg gccgacagcc
     1021 gctcgcgcag ctcggtgtcc aggttcgaga acggctcgtc gagcagcagc aggtcggggc
     1081 gcggcgccag cgcgcgcgcc agcgcgacgc gctgctgctg cccgcccgag atctcgtgcg
     1141 gatacttgtc gagcgccgcc gtcagcccga ccagcgcggc gagctccgcc acgcgcgcgt
     1201 tgcgctcggc gccgggcccg cgcagcccga agacgatatt gccgcgcacc gacaggtgcg
     1261 ggaaaagcgc gtagtcctgg aacaccatgc cgatgcggcg cttctcgggc gcgagcatcg
     1321 cgccgggtgc ggaaacttcc cggccgccga tgtgaatcgt gccgcgctgc gccgcctcga
     1381 agccggcaat gcagcgaagc acggtcgtct tgccgcagcc gctcgggccc aggaggcagc
     1441 cgatcgctcc gcgcggcagc gaaaacgaca ggtcgcgcac cacctggtgc gtgccatagg
     1501 cgtggctcaa ctcccggact tcgagaagcg gcgaggggct gttcacaggg ctaattatag
     1561 taattctcat tattgttaga atggcaaatc ctgaaagttc aaacgcttag agtcatctcg
     1621 atcgccgtcg cgctgcttct ggcgctgccg gtcgcgtcgg tcctcggcag cgtcctggcc
     1681 ggcggcggcg agacctggtc gcacctcgcc gccacgggcc ttgcgcgcta tgtcggcaat
     1741 acgttgctgc tgctggtcgg ggtggcatgc ggagtcatct cgatcggcgt gctgtgcgcc
     1801 tggatcgtga ccgtgtatcg cttccccggg cgcgatttcc tcgagtgggc gctgatgctg
     1861 ccgctcgcca tgccggctta cgtcatggct tacgcgtaca ccgactggct gcagttcgcg
     1921 ggcccggtgc agacgtcgct gcgcgcggcg acgggctggc aagcgcgcga atactggttc
     1981 ccggagatcc gctcgctggg cggcgcgatc gcgatgctct cgctcgcgct gtatccgtac
     2041 gtctacctca tcgcgcgcac cgcgttcctc gagctctcgc gcagcgccat cgaagccggc
     2101 cggctggccg gcctttcggc ctggggggcg ttctggcatg tcgccgtgcc gctcgcgcgc
     2161 ccggcgatcg ccgccggcgc ggcgctcgcg ctgatggaga cgctcgccga tttcggcacc
     2221 gtgtcgtact tcgccgtcga agtgttcacc accggcatct tcaaggcctg gctctcgatg
     2281 ggcgacaccg tcgcggcggc gcagctctcg agctgtctgc ttgccctggt cgtgctggtg
     2341 gtcgcgctcg agcgcgcggg ccgcggtcgc gccgcctacg ccgcgcccgg ccgccgtccc
     2401 gcgccaccgc agcgcctgca tggcggcgcg tcgctggccg cgctcgctgc ctgcgcggcg
     2461 ccgctcgcgc tcggctttgc gctgccggct gggctgctgg ggcgcctcgc ctgggacgac
     2521 gcgggcctgc tggtcagcgc gcgcctcgcc acgctcgtcg gcaacagttt cgcggttgcc
     2581 gggctcaccg cggcgctcgc ggtggtgctg gcgacgctga tggcctatgc cgcgcgcatc
     2641 gagcgcagcg gttgggtgaa cgtggccaac cgcgccgcga gcctgggcta tgccgttccc
     2701 ggcgcggtca tcgcaatagg cgtgctggtg ccgctcggcc gcttcgacaa cgcggctgcg
     2761 gcctggatcg agcacgcgtt cggcgtcaag atgggactgc tgctcaccgg cagcgtcgcg
     2821 gcgctgatct acgcctacct cgtgcgcctg ctggcggttg cgctgcagac caccgaagcc
     2881 ggcctggcga agatcacccc gagcatggag gacgccgcgc gctcgctcgg ggcttcgccc
     2941 gccggtgcgc tggcgcgcgt gcatgtgccg ctgctcgcgc cgagcatcgc cgcggcggcg
     3001 ctgctggtgt tcgtcgacgt cctgaaggag ctgccggcga ccttcgcgct gcggcctttc
     3061 aacttcgaca ccctcgcgat cgaggcctac aacctggcga aggacgagcg gctggccgaa
     3121 gccgccgcgc cgtccctggt catcgtcgcg gtcggcctga taccggtcct gtttgtgacg
     3181 aagcgctact tccaggcgac gcggtcgtag attttctggg agctcggctg gttcttgccg
     3241 agcaccgcta cgttgagcgc gtcgcgcttg aagtcgccca ggcggctgag caccgggttg
     3301 tcgatcctga cgccgggtac cgcgggccac tcgttgttgc cgtcggcgaa atagcgctgc
     3361 gcctcgtcgc tcgccaggta ttcgaggaag cgcaccgccg cctcgcggtt gggcgcgttc
     3421 ttgagcacgc cggcgcccga gatgttgacg tgcgtgcccc aggtcttctg gttcgggaag
     3481 acgacgccga ggcgctcgcc gatctgctgc tcgtcgggct tttgcgagcg cagcagccgc
     3541 gcgtagtagt actggttcga gatcgtcaca tcgcattcgc cggcggcgac cgccttcagc
     3601 tggtcggtgt cgccgccctt cgggtccacg gcgaggttgg tgcgcacgcc ctgcgcccag
     3661 gcctgggcct tctcctcgcc gaggtggtcg atcagcgcac ccatcaggga caggttgtag
     3721 acgttggtcg aggagcgcat gcagacgcgg cccttccact tcgggtcggc gagctcctcg
     3781 taggtctgga tctcgccggc cttcaccttc gccttgttgt aggcgatcag gcgcgcgcgg
     3841 gtggagaagc cgaaccacag gccgctcggc tcgcgcaggc tggccggaat gcggctttcg
     3901 agcattgcgc tcctggtcgg ctggaacagg ccgagctgct cggcgcgcca cagcctgccg
     3961 gcgtccaccg tgatcagcac gtcggcgggg ctgcgctcgc cctcgttgcg cacgcgctcg
     4021 atgagcgcgt cctcgccgcc ctcgatgcgg ttgatcttga tgccggtggc tttggtgaag
     4081 ccggtgtaga gcgcttcgtc ggtctggtag tgccgcgagg agtagaggtt caggactttg
     4141 tcctgggcgg cggcggggat cgcggcgaac agtgcggccg cggccaacat gatatttttc
     4201 cggaggaatt tgggcatgat gcccaaacga taatgactcc tattgccaat gtcaatgaga
     4261 ataattatcg atatcgagcc ttctcgcacc gttgaacctc acctgccaat agggcgtgtc
     4321 gaaacgctct atgcggacgc gggcgcctgg tttcggcgca tggatgaagc ggccgttgcc
     4381 gatgtacatg ccgacgtgcg agtacggctg gtcgcgcgtg ttgtagaaga cgaggtcgcc
     4441 cggcgccaga ttgggccgct tcacgggttt gcccagcctg ctctgggcct gcgcgttgtg
     4501 cggcaggggc agcccccagg cctgctcgaa ggcgtgcgcg atcaggccgc tgcaatcgaa
     4561 gccggtctcg ggcgagctgc cgccatagcg gtagcgcgct ccgagggcgc gcagcgcgta
     4621 ggcgagaggg ccctcgcgcg aagaagctcc ttgcgcggtc gtagcgagcg ccgggagagg
     4681 gaacaggcag agccagccgc agagcaggag aagctgcttc ataatttcca ggtgaacgac
     4741 gcgcaagctt gcgcgttgtg tgagtgggag ccgatgaagt gccattgatc cgatcagggt
     4801 tagcgatcac ttttctttgc gcggcgcagg cgtgggcgca aggcgccctt gagatcattc
     4861 ccctgaagca ccgcacggtc gatcaggtgc tgccggtgct gcgaccgctg cttgatccgg
     4921 gggcgacgct gagcgggcaa tccgggcagc ttttcgtgcg gaccagtcct gaaaacctgg
     4981 ccgagctcag gcaggcgctg gaggccatcg accggccctc ccgccggctg cagatcctgg
     5041 tgcgtttcga cgatgccctg gaatcctcca gccgcgacgt cgccgcgagc ggcaccataa
     5101 gtaatcgcgg ctcgcgggtg gagctgcgcg cgcaggaggc gcaggcgggc gcgaccgaac
     5161 ggatcgacca gcgtgtgcag gtagtcgagg gcggcacggc ctggatcacg accggacagt
     5221 cccgcacgct gccgcagcgc cagctcattc gaacgccagg cggcgtggtt tcccaggaga
     5281 cgttcgtcgt gcaggaggcc acgaccggct ttgccgtgtc gccgcgtctt tcgggcagca
     5341 cggtgcacct cgacatctcg ccgcagcgcg aggcgctggc tggaggcggc gccgtccagt
     5401 cgcagcgcat cgcgaccagc gcgagcggcc gcctgggcga atggttcgag atcggcggga
     5461 tcgccgcggc cagcgcacgc gatgagcgcg gcgtcgcttc ggctgcccgc accaccggcg
     5521 gcgagtcgcg ccggatatgg gtgaaggtcg aggagctgcg caactgacgc gtcctctcga
     5581 gcgcgcgttc gcggcagacg ggccgctcgc cgcgcggatt ccgggcttca agccgcgcgc
     5641 acagcaggtc gagatggcgg ccgcaatcga ggaagccatc gggcgcaacg gcgtgctggt
     5701 cgccgaggcc ggcaccggca caggcaagac cttcgcctac ctcgtgccgg cgctgctctc
     5761 gggcggcaag gtgatcgtct cgaccggcac caagaccctg caggaccagc tattcgaccg
     5821 cgacctgccc gcggtgcgcg aagcgctcgc ctcgggagcg agcgtcgcgc tgctcaaggg
     5881 gcgcagcaac tacgtctgtc tgtatcggct ggggcgcgcg cgcggcgagg cgcggctcga
     5941 aacgcgcgag caggtcgcgc agctcgcgcg catcgagcag ttctcccgcg cgagcgtcac
     6001 cggcgagcgc gccgaccttg ccgacgtgcc cgaggacgcg gccgtctggg cgcatgcgac
     6061 ctcgacgcgc gagaactgcc tcggccagga gtgccccgat tatcgcgact gcttcgtgat
     6121 gcgggcgcgg cgcaacgccc tggccgccga cgtggtggtg gtcaaccacc atctcttctt
     6181 cgccgacgtc gtgctgcgcg acgaaggcgt cgccgagctg ctgccggcct gcaacacgct
     6241 ggtcttcgac gaggcgcacc agctgcccga gacggcgcgc atgttcttcg gtgaaaccgt
     6301 gtcgagcgcg cagctggtcg agctgtcgcg cgacctgcgc gtggagctgc gcgcggccgg
     6361 cggcgcgtcg cccgagctcg acgcgctggc gggccggctg gagaaggcgg cgcgcgacct
     6421 gcgcctcgcc ctcggcgagg cgggcacgcg cctcgcttgg tcacaggcgc ttacgctgcc
     6481 aggttttccg gattctctga aaagacttac ccaggccctg cgcgacgtcc aggcgccgct
     6541 cgctgcgcag gccgagcgtt ccgagggact cgactcttgc gcgcgccggg cgggcgcagc
     6601 gtcggcgctt gtgctgcgga tgcacgagga gagctcgggc gaggtgcgct gggtggaagt
     6661 gttcggacag gccgcgcagc ttcacctgac gccgctttcg ccagcggagc tgttccgcaa
     6721 gcagatgtcg gaccatccgc gcgcctggat ctttacctcg gccaccctgg cggtgggcaa
     6781 tgacttcagc catttcacgc gcgagctggg ggttgccgag gccgagactc gccgctgggc
     6841 gagcccgttc gatttcgcgc gccaggcgct gctctacctg ccgcgcggcc tgccggccga
     6901 tcccaatgcc cccggcttca ccgaggcggt ggtcgaggcg gccctgccgg tgctgcgcgc
     6961 gagcggcggg cgcgccttcc tgcttttcac caccctgcgg gcgctgcgca aggcgcacga
     7021 gctgctgcgc gatcgcatcg agtttcccct cctcgtgcaa ggcaccggct cgcgcagcga
     7081 gctgctcgag cgcttccgcc gcctgggcaa cgcggtgctg ctcggcagcc agtccttctg
     7141 ggagggcgtg gatgtgcgcg gcgaggcgct atcggtggtg gtgatcgaca agctgccttt
     7201 cgcgccgccc gacgacccgg tgctcgcggc gcgcatcgac ggactgaagg cgcgcgaggg
     7261 caatccgttc accgagcttc agctgccgcg ggcggtgctg cagctcaagc agggcgccgg
     7321 ccgcctgatc cgcgacgaga ccgaccgcgg cgtgctgatg ctgtgcgatc cgcgcctcgt
     7381 gtcgaagtcc tacggccgcg gcatcatccg cagcctgccg ccgatgaaac tcacacgcga
     7441 gctgccggag gtcgagggct tcttcggtta ggagagctcg tccgcgacgt ggtagcggac
     7501 gatgtcgtcg aagctcggat cggcgcgaaa gccgagggcg agcgagcgtt tcgcgtcgaa
     7561 gttgcgcggc cagccttcga cgatgcgcat gatggtcggg tcgggctcgc gccggatgag
     7621 gcgcaccgcc ttctcgccgc ccacccggcg cagcgagtcg atttcctcgg cgatggtggc
     7681 cgacagcccc ggcatcgaca ggttgcggcg cgtgcctagc agttcggtat tgagctttga
     7741 agcatggatc agaaattcta tggccgcgcg cggcgacgcg aaccagtggc gcaccgtgtc
     7801 gggcaccggc agcaccgctt cctggccggc cagcggctcg cgcaggatgc cggagaagaa
     7861 gcccgaggcg gccttgttcg gctttcccgg gcgcacgcag atcgtcggca ggcggatgcc
     7921 gatgccgtcg aagaagccgc gccggctgta gtccgagagc agcagctcgc cgatcgcctt
     7981 ctgcgtgccg tagctggtga gcggcgcgtt caggaactcg tcgccgatcg cctcgggaaa
     8041 gggggcaccg aagacggcga tggaggaggt aaagacgagt ttcggcttgt atccatcacc
     8101 gattttgcgt accgcctcaa acaggcgccg cgtgccgtcg aggttgatgc ggtagccctt
     8161 ctcgaagtcg gcctcggcct cgcccgagac gatggcggcg aggtggaaga tcaggtccgg
     8221 gcgcttcgcc accagcttct cggcttctcc cggtacggag agatccgaaa ccatggagct
     8281 gccttcgaac ggcacgacgt cgtgcaggat cagctctccc atgccttccc tggcgagccg
     8341 cgccgtgagc ttgcggccga tcatgccggc cgcgccgatg accagaatcc gcttgctcac
     8401 cagtccggat cccagatgcg ccaccagggc gcatcgcgcc ggtcgccgcc ggccttgaga
     8461 tagcggctgt tggggaagtt ggcgcgcatc acgcggtcgg cgccatcgcg cagctcgtcc
     8521 ataccgaggg cgtcgtaggc cttgaccagc acgaacaccg cttcctcgag cgccggcgcg
     8581 ttggggtaga agcggatcgc gtactgcgcc cggttcgccg ccgcgaggta ggcgccgcgc
     8641 ttcatgtagt agcgcgcgac gtgcacttcg tgcgaggcga gcgcgttgac caggaagtac
     8701 atgcgcgcct cggcgtcctc ggcatacttg gattcggggt agcgcgtcac cacttccttg
     8761 aaagcgtcga acgcctcgcg cgatgccttc gggtcccggt cggtcacgtc cggcgtcgcg
     8821 atctgggcga tggcgttctg ttgcaggccg aagttgatca tgcccttgag ataccaggcg
     8881 tagtcgatgc tggggtggtt cgggtagagc ttgatgaagc ggtcgacggc gccgatggcg
     8941 ctggcgcgct cgtcgtcctt ccagaagcag taggcgattt cgagctgcgc ctgctgagcg
     9001 aagcggccat agggatagcg cgcctcgagc ttttcgaggt acttgaccgc cttgggccag
     9061 tccctgcccg cctgggcgtc cttggcctcg ccgtagaggc gctgcgccga ccagccgatg
     9121 gtctcgtcgt cgggcgagct cgacaggagg ctgcagccgc cggcgagcag gacggcgacg
     9181 agaagcgccg cggaaagtaa actgcgctgc atgaagggcg acaattatag cgacgactgg
     9241 gacgccggct tcgcagcacc gatgagcgag acggtgcccg ccgggctcgg cgggctgagg
     9301 ctggatcagg tgctggtgcg cctgttcccg cagtactcgc gcaaccggct ccaggcgtgg
     9361 ctgaaatccg gccacattcg cgtgaacggt gcttctctgg aagcaaaaca cccggtcgcg
     9421 ggcggcgagc ggatcgtgct cgagcccccg ccgccgccgg cggtcgtgcc gcaggcgcag
     9481 cgcatgccgc tcaagatcgt gcacgaggac gcgtccctca tcgtgatcga caagccggcc
     9541 gggctggtag tgcatcctgg cgcgggccag cccgacggca cgctgctcaa cgggctgctt
     9601 gcgcactcgc ctgcgttagc gggcgtgccg cgcgccggca tcgttcaccg gctcgacaag
     9661 gacacgagcg gcctgctcgt cgtggcgaag acggtcgtgg cgcaggcgag cctggtgaag
     9721 cagctcgccg accgcagcat gcggcgcgtc tacctggcgg tggtgcaggg cgatccgccc
     9781 gcgagcggcg tgatcgacgc gccggtcggc cgcgacgtgc gcgcgcgcgt gcgcatggcg
     9841 gtgaggcacg gcggcaagga cgcgcgcacc tcgtaccgcg tgctggagcg cttcggccat
     9901 gccgcgctcg tcgaatgccg gctcgaaacc ggccgcaccc accagatccg cgtgcatttc
     9961 cagcacatcc gccatcctct ggtgggcgac acggtctacc gccgcggcac gcgccacggc
    10021 ctggaattcc cccggcaggc gctgcatgcc gccgagctct cgctcaagca tccgcagacc
    10081 ggcaagctga tgacctggca ggcgccgctg ccggcggaca tgaagcgcct gctgaagcgc
    10141 ctcgaagatg attgagcccg actggcccgc gcacgcgcgc gtgcgcgcgc tgatgaccac
    10201 gcggccgatg ggcgacatgg ccggcgaggg caggcgctcg ctggaggggc tgctgcccgg
    10261 cgagccggcc tggctgacgc aggttcacgg cgtctcagtt acggaaatag attccccgag
    10321 ccgctcgctc aaggcggatg ccgcagtcag ccgaaaggct ggaaatgtct gcgtggtggg
    10381 cgctgcggat tgcatgccgc tgctgctgag cgacaagtcc ggcagcgtgg ttgcggcggc
    10441 gcacgccggc tggcgcggcc tgagcgcggg cgtcatcgag gccgccgtgc gcgcgatggg
    10501 tgtgccgccg cgacatgtgc tggcctggct cggtccggcg atcggcccgc gcgcctacga
    10561 gatcggcgag tacgtccgcg ccgccttcgc ctcgtacccc gacgcgttct ccgcgccgac
    10621 gcggccgggc cactggctgc tcgatctgta cgcggtggcg cgcgagcgac tcggcgacat
    10681 gggcgtcacg cagatccacg gcggcggctt ctgcacctac accgacgccg ccaggttcta
    10741 ctcctataga agagacggca caaaagcgcg catggcggcg ctaatctggc tcacgtagaa
    10801 tccccacccc catgacgctc ggttggatcc tcgccgcgag cttcgccggc gggacgctgt
    10861 ccgccgcgct cgctgccttc tccctgtacc tgcgcgcgag ctggatcccg atgctcgtgt
    10921 cgttcgccat cggcacgctg ctcggcgccg cgttcctcga ggtgatcccg gcggccttcg
    10981 accagggcga cgcgcacgag gcggcgagcg ccattctcgg cggcatcttc ggcttcttcg
    11041 tgctggaaaa gctgatgctg tggcgccact gccacaccga ggactgcgaa gcgcacgatc
    11101 aacatgcaac acatgaccat ggccgcagcg gcgccttgat catcgtcggc gacacgatcc
    11161 acaacttcgt cgacggcgtg ctgattgccg ccgcgttcct gcagggtatc gagatcggca
    11221 tcgtcgccgc ggtggcgatc atcgcgcacg agatcccgca ggaagtcggc gacttcctga
    11281 tcctgctgca ctccggctat acgcgcgccc gcgcgctcgc catgaacctg ctctcgtcgc
    11341 tcgccacgct cgtcggcggc ttgctcggct actacagcct gcaggccttc gagggcctgg
    11401 aacccacgct gctcggcatc gtcggggcga gcatgatcta cgtcgcggtc gccgacctga
    11461 ttcccggcct gcaccgccgg ccgcagctgc gcgacggcgc cacgcagacc ctgctgatcg
    11521 ccctggggat cggctgcatc gtgctggtcc gcgtcatggt cgcccactag tcgaaaagag
    11581 ctaaaattat cccgcaatgc aacatgcggg gtctccggag gcgctgctcg ccgccatcgc
    11641 ccagtcgggc gacgaggtaa cgcagggctg gatgcggctc ttgtcgagcg ccgccggcga
    11701 cgccggctgg ctcgccgacc tgcagcgcaa cagcgcgcgt ttcggctcgg tgcaggccgc
    11761 ctacttcgag aagcagacgc ggctctggac cgggctgctg tcgggaagcg acgagcccgt
    11821 agccgcgccg cgcgccggcg accgccgctt ctccggcaag gagtggcgag acaaccccta
    11881 ctacaactat ttgcgccagt cctatctgct cgccgcgagc tatatcgagg agctggtcga
    11941 gggctgcgag ctgaagccgg tggcgaagga gcggctgcgt ttcgcggcgc gccagtggat
    12001 cgatgcgatc agcccggcca atttcgcggc gaccaatccc gcggcgctca gtcaggcgat
    12061 cgccacccag ggcgagagcc tgacccgcgg cctcgccaac ctgctcggcg acgtgcagaa
    12121 aaagcgcatc agccagagcg acgaggcggg cttcgaggtc ggccgcaaca tcggcgtgac
    12181 gcccggcagc gtcgtcttcg agaacgagct gatccagctg atccagtatc gggcggcgac
    12241 cgaaaaggtg gcggcgcggc ccctggtcat agtcccgccc tgcatcaaca agtactacat
    12301 cctcgacctg caggcggaga attcctttgt cgcccacgcg atccgcgccg ggcacacggt
    12361 gttcatggtc tcctggcgca acgtcgggcc ggagcagggc catctcacct gggacgacta
    12421 cctcgagaag ggcgtcttca gcgcccttcg cgcggcgagg gaaatcgcgg gaagcgagca
    12481 ggtgaacgcg ctcggcttct gcgtcggcgg caccctgctt ggcgcagcct tatctgtttt
    12541 atctcggaag aatcaaaatc cggtggcgag cgctacgttc ctcgccacca tgcttgattt
    12601 ctccgacact ggccagatcg gactgttcgt cgacgagtcg agcgtcgccg cgcgcgaggc
    12661 cgcgatcggc cgcggcggcg tcttccccgg ctccgacctt gcgttcgtgt tttccagcct
    12721 gagggcgaac gacctgatct ggccgtatgt ggtcaacaac tacctgctcg gcgaggcgcc
    12781 ggcggcgttc gacctgctct actggaacgc cgacagcacg aatctccccg ggccgatgta
    12841 ctgctactac ctgcgcaaca cgtacctgga gaacaacctg cgcaagccgg gtgcgctgct
    12901 gaactgcggc gtgccggtcg acctgggcga ggtcaggctg ccgctgttcg tgctggcgac
    12961 gtgcgaggac cacatcgtgc cgtggcgctc cgcgtaccgc acgatcggac tgctcggcgg
    13021 caccgacaag acgttcgtcc tgggcgcgag cggtcacatc gcaggcgtgg tcaatccgcc
    13081 cgccaaaaag cgtcgcagct actggacagg cgctccctac cccaaagacc ccaatgagtg
    13141 gctggataag gcgaaagaaa tccccggcag ctggtggccg gtgtggagcg agtggctctc
    13201 ccggcacaaa ggcggcgagc gcgaggcacc ccgcgccgcc ggcaatgtcc aatacaaggc
    13261 tgtcgaagcg gcgcccggcc gctacgtgaa gcaaagactg cactaggaga ttcccatgga
    13321 agacatcgtc atcgtcggcg cagcgcgtac cgccatcggc aagttcggcg gcagcctcgc
    13381 caagaccccg gcctccgacc tcggcgcgct gatcatccgc aaggtgctcg agcgcgccgg
    13441 cgtcgccccc gagcaggtct cggaggtgat catgggccat gtgcttacgg ccggagtcgg
    13501 gcagaacccg gcgaggcagg cggcgatgaa ggccgggctg cccaacatga tccccgcctc
    13561 gaccatcaac gtggtctgcg gctcgggcct caaggcggcg atgctcggcg cgcaggccat
    13621 cgccaacggc gattcggaca tcgtggtggc gggcggccag gaatcgatga gcctctcgcc
    13681 gcacgtgctg aacggctcgc gcgacggctt tcgcatgggc gacgccaagc tggtcgacag
    13741 catgatcgtc gacggcctgt gggacgtcta caaccagtac cacatgggcg tgaccgccga
    13801 gaacgtggcc aaggaatacg cggtgacgcg cgaagagcag gacgagttcg cggtggcgag
    13861 ccagcacaag accgaggcgg cgcagaaggc cggcaggttc aaggacgaga tcgtgacggt
    13921 ggaaattgcg tcgcgcaaag gcccggtgat gttcaccgac gacgagtttc cgcgccacgg
    13981 cacgacgctc gaagcgatga agggcctcaa acccgctttc gacaagcagg gcacggtgac
    14041 cgccggcaac gcgtcgggga tcaacgatgg tgcggcggca attgttttgt gtagtttgaa
    14101 aaaatccaaa gaattgggtc tgaaaccgat cgcccgcatc aaagccttcg cctccgccgg
    14161 cgtcgatccg aagtacatgg gcatgggtcc ggtgccggcc tccaagcgct gccttgcgcg
    14221 cgccgggtgg cagccgaagg atctcgacct gatggaaatc aacgaggcct tcgccgcgca
    14281 ggcgatcgcc gtcaaccggc agatgggctg ggacacgaag aaggtcaacg tcaacggcgg
    14341 ggcgatcgcg ctgggtcatc cgatcggcgc ctcgggagcg cgcatcctcg tcacgctgct
    14401 gcacgagatg caaaaacgcg atgccaagaa gggtttggca tctctctgta ttggtggcgg
    14461 gatgggcgta gcgctcgccg tggaaaggac atgatgggaa cgcagtctga aatcgtcgca
    14521 gaccccaaga ccgggcaacc ggtcgcgact cgcatcgcct tcgtctcggg cggcatgggc
    14581 ggcatcggca cggcgatctg ccggcgcctg ggccgcgcgg gtcataccgt ggtggcgggc
    14641 tgcctgcccg aatacgagaa aaaggcggag tggctggcgc agatgcgcgg cgagggaatc
    14701 cgcgtgcacg ccgccgaagg cgatgtcagc gaattcgact cctgcgccga gatgttctac
    14761 cacatccgct cggtggtcgg cccggtggaa atcctggtga acaacgccgg catcacgcgc
    14821 gactcggtgc tcaagcgcat gaccgagcag gactggatgg cggtgatcaa caccaacctg
    14881 aacagcgtgt tcaacgtcac gcgccaggtg atcgacggca tgagcgagcg cggctggggg
    14941 cgcatcatca acatctcgtc ggtgaacgcc ctcaagggcc agttcggcca gaccaactat
    15001 tcggcggcga aggccggcat ggccggcttc tccaaggcgc tggcgcagga agtggtaaaa
    15061 aaaggcgtca ccgtcaacac ggtgtcgccg ggctacgtcg aaaccgacat ggtgatggcg
    15121 atccgcaagg aaattctcga gcagatcgtg gcctcgattc cgatgggccg gctcgccaag
    15181 ccggaggaga tcgcggcggt ggtggcgttc ctcgcctccg aggaggcggg ctacatcacc
    15241 ggcgccaaca tctcggtaaa tggcggcatg cacatgatgt agcattagtt ctcctgagga
    15301 ggagacatgg ccggcaagaa ggtcgccgtc atcaccggcg gcatgggcgg attgggcgag
    15361 tcgatcacca ccaagatggc cgacgcgggc taccgcgtgg tcgttaccta ctcgccttcg
    15421 aacaccaaat acagggcgtg gctggaggag atgaaaggcc gcggctattc gttctccgcc
    15481 ttcccggtgg acgtggtgga cttcgacgac tgcgcgcgca agtgcgcgca gatccagggc
    15541 gagtgcggcg cgatcgacat cctggtcaac aacgccggca tcacgcgcga catgacgttc
    15601 aagaagatga ccaaggccga ctgggacgcg gtgatgcgca ccaacctcga ctcgtgcttc
    15661 aacatgacca agcaggtgat ggacggcatg gtcgagcgca cctgggggcg catcatcaac
    15721 gtctcctcgg tgaacggcca gaagggcgcc ttcggccaga ccaactactc ggcggcgaag
    15781 gccggcatcc acggcttcac caaggcgctg gccctcgagg tggcgcgcaa gggcgtgacc
    15841 atcaacacca tctcgccggg ctacatcggc accaagatgg tcaccgccat cccgaaggag
    15901 atcctcgact cgaagatcct gccgcagatt cccaccgggc ggctgggcaa gcccgacgag
    15961 gtggcggggc tgatcattta cctgtgctcg gacgaggccg ccttcgtcac gggcgccaac
    16021 atctccatca acggcggcca gcacatgtac taaggagtag aatttcctcg tggcgtctat
    16081 tcccgaaacg aagtcggaaa accacctccg gctgatcaag aagtacccca accggcggct
    16141 gtacgacacc aagacgtcga gctacatcac gctcgccgac gtcaagcaga tggtgctcaa
    16201 gcaggaggac ttccaggtgg tcgacgccaa gagcggcgaa gagctgacgc ggcagatcct
    16261 gctgcagatc atcctcgagg aggagcaggg cggcctgccg atgttctcct ccgacctgct
    16321 gtcgcagctg atccgcttct acggcaacgc gatgcagggc ctgatgggca cctacctcga
    16381 gaagaacatc cgcgccttcc acgacatcca gaagacgctg caggagcagt cggcgcgcat
    16441 gtacggcggc gacaccacgc gcggcacgca ggacctgtgg gcgcagttca tgaacctgca
    16501 ggggccggcg atgcagaccc tgatgcaggc ctacgtcgag caaagccaga agatgttcgc
    16561 gcagttccag gaccaggtgc agaaccaggc gcgcaacatg ttcaccggct tcaccttccc
    16621 cggcttcac
//
LOCUS       DASYJA010000311         2547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_136981, whole genome shotgun sequence.
ACCESSION   DASYJA010000311 DASYJA010000000
VERSION     DASYJA010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136981"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VGO02_06890"
     CDS             <1..551
                     /locus_tag="VGO02_06890"
                     /inference="COORDINATES: protein motif:HMM:NF015656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HEV7477446.1"
                     /translation="TKVTIDISGCGYTVDDLQKVLFERYNIQTEKSTFNTLTLLLTIG
                     TTRSKVSRLYDALMRIAREGRQPRRLIQTPDIPAFTKLKYLPRDAYYCGGELLPVLDE
                     RERVNRGLAGRICADQIVPYPPGIPVLVPGQLITRKVAEYVANLLRSQKRMEVHGIVH
                     EGYVPCIRVLKPDEEKSLKRLA"
     gene            complement(548..1321)
                     /locus_tag="VGO02_06895"
     CDS             complement(548..1321)
                     /locus_tag="VGO02_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV7477447.1"
                     /translation="MKFVAGLLSVLLAACAANPPAAEVAPGGTLRVGIGVGPVPSAFW
                     ATRDPASGEPRGVTVDLARAMAKDLGVPLRLVSYANSGEVTAAGPKGEWDVAFMPVDE
                     VRARMVDFGPAYYLTESTYLVAAGSPIRTLADVDRPGVRVAGVEGTTTARSAARSLKN
                     NTVKTYRTADELLELIRSGNADAIALGRESLAGMQAKLPGSRILDGHFQATGTAVAVP
                     KGRAAALAYVTTFVEKAKKDGTVRRALDAVGLKDSPVAP"
     gene            1408..>2547
                     /locus_tag="VGO02_06900"
     CDS             1408..>2547
                     /locus_tag="VGO02_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEV7477448.1"
                     /translation="MSHVMNTYARQPVAFVRGEGVWLWDEAGKKYLDALAGIAVNTLG
                     YAHPRFLKAINERVKSGVIHTSNLFRIPDQEAAADRVAEITGLDEVFFCNSGLEATEA
                     SIKVARKYGHDKGIAEPAIIVMEKAFHGRSLATLSATGSRKVQAGFEPLVQGFVRVPL
                     NDLDAVRQIAERNRSIAAVMIEPIQGEGGINVSRLEYLRGLKQVCDESGWLFISDEVQ
                     CGMGRSGKWFIYQHAGFQPDVVALAKGLASGVPVGACVVGNRAKGVFKPGNHGSTFGG
                     NPLAMTAVRTTIDTMKEDGLLQNAERVGGVIRQGLLDAFAGMKGVAEVRGMGMMIGIE
                     LQEACGELVKQALEAGLVINVTADNVVRLLPPLVMSEAEGRQVVER"
BASE COUNT          433 a          875 c          853 g          386 t
ORIGIN      
        1 cgaccaaggt gaccatcgac atctccggct gcggctacac ggtcgacgac ctgcagaagg
       61 tgctgttcga gcgctacaac atccagaccg agaaatcgac cttcaacacg ctcaccctgc
      121 tgctgacgat cggcacgacg cgctccaagg tgtcgcgcct gtacgatgcg ctcatgcgca
      181 tcgcgcgcga gggtcgccag ccgcggcgcc tgatccagac gccggacatc cccgccttca
      241 ccaagctgaa atacctgccg cgggacgcgt attactgcgg cggcgagctg ctgccggtgc
      301 tcgacgagcg cgagcgcgtc aaccgcgggc tcgccgggcg catctgcgcc gaccagatcg
      361 tcccctatcc gcccggcatc ccggtgctgg tgcccggcca gctgatcacc cgcaaggtcg
      421 ccgagtacgt cgccaacctg ctgcgctcgc agaagcgcat ggaggtgcac ggcatcgtgc
      481 acgagggcta cgtgccctgc atccgcgtgc tgaagcccga tgaggaaaaa agcctgaagc
      541 gcctcgccta aggcgcgacc ggagaatcct tcagcccgac ggcatcgagc gctcggcgca
      601 cggttccgtc cttctttgcc ttttccacga aggtcgtgac ataggccagc gcagcggcgc
      661 gacccttggg taccgcgact gccgttcccg tcgcctgaaa atgtccgtcg aggatcctgg
      721 agccggggag cttcgcctgc atgccggcga gcgactcgcg cccgagcgca atggcgtcgg
      781 cgttgccgct gcggatcagc tcgagcagct cgtcggcggt gcggtaggtc ttcaccgtgt
      841 tgttcttcag cgagcgggcg gcgctgcgcg ccgtggtggt cccttcgacg ccagccacgc
      901 gcacgccggg ccggtcgacg tcggcgagcg tgcggatcgg cgagccggcc gcgaccaggt
      961 aggtgctctc ggtgaggtag taggccgggc cgaaatccac catccgcgcg cgcacctcgt
     1021 ccaccggcat gaaagcaacg tcccactcgc ctttggggcc ggcggcggtc acttcgccgg
     1081 agttggcgta cgacacgagg cgcagcggca cgccgaggtc tttcgccatc gccctggcca
     1141 ggtcgacggt cacgccgcgc ggctctccgc tcgccgggtc gcgcgtcgcc cagaacgccg
     1201 agggcaccgg cccgacgccg atgccgacgc gcagcgtccc gcccggcgcg acttcggcgg
     1261 cgggcgggtt ggcggcgcat gccgcaagca ggacgctcaa caatccagca acaaacttca
     1321 ttgctgcatc ctacacaaga cggtttgccc ctgataggat tcccgaaacg gcggcctttg
     1381 ccgccgtttt tattgggaga aagcgccatg agccacgtca tgaacaccta cgcccggcag
     1441 ccggtggcgt tcgtgcgcgg cgaaggcgtc tggctgtggg acgaagccgg caagaaatat
     1501 ctcgacgcgc tcgccggcat agcggtgaat acgctcggct acgcccatcc caggtttcta
     1561 aaagcgataa atgagagggt caaaagcggc gtcatccaca cctccaacct gttccgcatc
     1621 cccgaccagg aggcggccgc cgaccgcgtc gccgagatca ccggcctgga cgaggtgttc
     1681 ttctgcaact ccggcctcga ggcgaccgaa gcgtcgatca aggtcgcccg caagtacggc
     1741 catgacaagg gcatcgccga gccggcgatc atcgtcatgg aaaaggcgtt ccacggccgc
     1801 tcgctcgcca cgctctccgc caccggcagc cgcaaggtgc aggccggctt cgagccgctg
     1861 gtgcagggct tcgtgcgcgt gccgctcaac gacctggacg ccgtgcggca gatcgccgag
     1921 cgcaaccgca gcatcgccgc ggtgatgatc gagccgatac agggcgaggg cggcatcaac
     1981 gtctcgcgcc tggagtacct gcgcgggctc aagcaggtgt gcgacgagag cggctggctg
     2041 ttcatctccg acgaggtgca gtgcggcatg ggccgcagcg gcaagtggtt catctaccag
     2101 cacgcgggct tccagcccga cgtggtagcg ctcgccaagg gccttgcctc cggcgtgccg
     2161 gtcggcgcct gcgtcgtcgg caaccgcgcc aagggcgtgt tcaagcccgg caaccacggc
     2221 tcgaccttcg gcggcaatcc gctcgccatg acggcggtgc gcaccaccat cgacacgatg
     2281 aaggaagacg gattgttaca gaacgcggag cgcgtcggcg gcgtgatccg ccagggcctg
     2341 ctcgacgcct tcgccggcat gaaaggcgtg gccgaggtgc gcggcatggg catgatgatc
     2401 ggcatcgagc tgcaggaggc ctgcggcgag ctggtgaagc aggcgctcga agccggcctg
     2461 gtgatcaacg tcaccgccga caacgtcgtg cgcctgctgc cgccgctcgt gatgagcgag
     2521 gccgagggcc gccaggtggt cgagcgc
//
LOCUS       DASYJA010000312         3137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1589725, whole genome shotgun sequence.
ACCESSION   DASYJA010000312 DASYJA010000000
VERSION     DASYJA010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589725"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VGO02_06905"
     CDS             complement(<1..950)
                     /locus_tag="VGO02_06905"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV7477449.1"
                     /translation="MSLLLEALKKAEKAKEEAQRRARGESGDAEPAAAAQAAPGGAAQ
                     LALEGEPAPDEATRHVTTRAELPDISAPLDIEREDLGPKAQPREALRLESQKAPPRRP
                     GVQPASQAAAPQDAERATARKVFEAKFREPNPKLPFYITLASLSVFAIGVVVYFWYQL
                     RPPYPLVNSNPVRSTAEAQVAAAPLASAPPAASSAASASSAIPGLPSSPPAPPIQPAT
                     RAPSAPPVERPLLSPAPRLAAPSINSPAGTPVRPLPEVSVTRAPVQVHPKVDAGYAAY
                     QAGDLAKARGEYEQALADEPGNRDALLGLAAVDTRSGRYEA"
     gene            complement(964..2742)
                     /locus_tag="VGO02_06910"
     CDS             complement(964..2742)
                     /locus_tag="VGO02_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HEV7477450.1"
                     /translation="MKPGIRAGLSLCAQLCCGALLLAACAQTPVKPAATHLRAPPPSA
                     EGQIPPPVQVAPLLPKPRPAGRVETYSVVVNNVRVQDLLFALARDARINVDIHSDLSG
                     TVTLNAIDQTLPQLLARIARQVDMRYEIEGQNLSVMRDTPFLRSYKIDYLSAARNVKM
                     SSLASTQFASSNSASGAATTSGTSTGATAQVDVTSENKLWESIVVNVKDILRETDKVI
                     PKLQPAAAVPVGAVIPAPVAGAALAVPQQSVEFIEVASVIANRESGILFVRATSRQHE
                     KVQEFLDQVLVGAKRQVLIEATIAEVQLNNQYQRGIDWQRLRSGATTTGRPAFGTGSS
                     GVEFGQNSTGTPAAVSTTAFVVGGAISSLNFNFALSLLESFGDVKVLSSPKLSVLNNQ
                     TALLRVTRDIIYFSITPAQITVSGGSGSVTIPPSFTTTPLVAAEGFMMSVLPQINEAD
                     TIVLNVRPTIRRRVGNATDPNPALITPNLIPIFETREFDSVLRLQSGQIAVLGGLMQD
                     QRSRTEDTIPGINAIPGLGELLEQRSESNTKTELVIFLRATVVREPSIDGDYRALRGE
                     LPGDDFFLKPNPARVAPPIGPGEAQR"
     gene            complement(2814..>3137)
                     /locus_tag="VGO02_06915"
     CDS             complement(2814..>3137)
                     /locus_tag="VGO02_06915"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEV7477451.1"
                     /translation="LLAQRLAATRELLGRAPDQSYAIELFITGNTDPARMERFLLRAR
                     EQVRLDELAVIPMAEGGQYRLRVVYGEFASREAALEAERRLPARYQDAFRTSPRSFAE
                     LRGQI"
BASE COUNT          407 a         1053 c         1170 g          507 t
ORIGIN      
        1 gcctcgtagc ggccgctgcg cgtgtcgacg gcggccaggc cgaggagcgc gtcgcggttg
       61 cccggttcgt cggcgagcgc ctgctcgtat tcgccgcgcg ccttggctag gtccccggcc
      121 tgataggccg cgtaacccgc atcgaccttg gggtgcacct gcaccggcgc gcgggtgacg
      181 ctgacttcgg gcagcggacg caccggcgtg ccggcgggcg agttgatcga gggcgccgcg
      241 aggcgcggtg ccggcgagag gaggggacgc tcaaccggcg gggcgctggg cgccctggtt
      301 gccggctgga tggggggagc cggcgggctg ctcggcagcc ctggaatggc gctcgatgcg
      361 ctcgcggcac tgctcgcggc gggcggggcg gacgccagcg gcgccgcggc gacctgggcc
      421 tcggccgtcg agcgcaccgg gttcgagttg accagcgggt agggcgggcg cagctggtac
      481 cagaagtaca cgacgacgcc gatggcgaag acgctgagcg acgccagcgt gatgtagaag
      541 ggcagcttcg ggttgggctc cctgaacttc gcctcgaaga ccttgcgcgc ggtggcgcgc
      601 tcggcgtcct gcggcgcagc cgcctggctc gcgggctgca cgcccggacg gcgcggcggc
      661 gccttctggc tttcgaggcg cagcgcctcg cgcggctgcg ccttcgggcc gaggtcctct
      721 ctctcgatgt cgagcggcgc ggagatgtcg ggcagctcgg cgcgcgtcgt gacgtgcctg
      781 gttgcctcgt cgggcgcggg ctcgccttcg agcgcgagct gggcggcgcc tccgggcgcc
      841 gcctgcgcag cggccgcggg ctccgcgtcg ccggattcgc ctcgggcgcg gcgttgcgcc
      901 tcttccttgg ctttctcggc tttcttgagc gcttcgagca gcaggctcat gtcgttcctg
      961 cgctcagcgt tgcgcctcgc ccgggccgat cggcggcgcg acccgcgccg ggttcggctt
     1021 gaggaagaaa tcgtcgcccg gcagctcgcc gcgcagcgcg cggtagtcgc cgtcgatgct
     1081 cggttcgcgc accacggtgg cgcgcaggaa gatcaccagc tcggtcttgg tgttggattc
     1141 gctcctctgc tcgagcagct cgcccagccc gggaatcgcg ttgatccccg gaatcgtgtc
     1201 ttcggtgcgc gaacgctggt cctgcatcag gccgccgagc accgcgatct ggccgctttg
     1261 cagcctgagc accgagtcga actcgcgcgt ctcgaaaatc gggatcaggt tgggcgtgat
     1321 cagcgccggg ttggggtcgg tggcattgcc gacgcgccgc cggatggtcg gccgcacgtt
     1381 gagcacgatg gtgtccgctt cgttgatctg cggcagcacg ctcatcatga agccctcggc
     1441 ggccaccagg ggcgtggtgg tgaacgacgg cgggatggtg acgctgccgc tgccgccgct
     1501 gaccgtgatc tgcgcgggtg tgatgctgaa gtagatgatg tcgcgcgtca cccgcagcag
     1561 cgcggtctgg ttgttgagca ccgacagctt cgggctcgac agcaccttca cgtcgccgaa
     1621 cgattcgagc aggctgagcg cgaaattgaa attcaggctg ctgatcgcgc cgcccaccac
     1681 gaaggcggtg gtgctgaccg ccgccggcgt gccggtggag ttctggccga actcgacgcc
     1741 gctcgagccg gtgccgaacg cgggacggcc ggtcgtggtg gcgcccgagc gcaggcgttg
     1801 ccagtcgatg ccgcgctggt actggttgtt cagctgcacc tcggctatcg tggcctcgat
     1861 cagcacctgg cgcttggcgc cgaccagcac ctggtcgagg aattcctgca ccttctcgtg
     1921 ctggcgcgag gtggcgcgca caaagaggat gccgctttcg cggttggcga tcaccgaggc
     1981 gacctcgatg aactcgaccg actgctgcgg caccgccagc gccgcgcctg cgaccggggc
     2041 ggggatcact gcccccaccg gcacggcggc ggccggctgc agcttgggga tgaccttgtc
     2101 ggtctcgcgc aggatgtcct tcacgttgac gacgatcgac tcccacagct tgttctcgga
     2161 ggtgacatcg acctgcgcgg tcgcgccggt gctcgtgccg ctggtggtcg ccgcgcccga
     2221 ggccgagttg gaggaggcga actgggtcga ggcgagcgag ctcatcttca cgttgcgcgc
     2281 cgcgctcagg tagtcgatct tgtaggagcg caggaacggc gtgtcgcgca tcacgctcag
     2341 gttctgaccc tcgatctcgt agcgcatgtc gacctggcgc gcgatgcgcg ccagcagctg
     2401 cggcagcgtc tggtcgatcg cgttcagcgt caccgtgccg ctgaggtcgg agtggatgtc
     2461 gacgttgatc ctggcgtcgc gcgccagcgc gaacagcagg tcctgcacgc gcacgttgtt
     2521 caccaccacg ctgtaggtct cgacgcgccc ggccggccgg ggcttgggca gcagcggcgc
     2581 cacctgcacc ggcggcggaa tctggccctc ggcgctcggc ggcggggcgc gcaggtgcgt
     2641 cgccgccggc ttcaccggcg tctgggcgca ggcggcgagc agcagcgcac cgcagcacag
     2701 ctgcgcgcac aacgacaggc cggcgcgaat ccccggtttc atcccatgct ccctcctgcg
     2761 gcaagcatag catccggggg agggcgcgac cctcggcagg acagcgggcc tgactaaatt
     2821 tgaccgcgca actcggcaaa actgcgcggc gaagtgcgga atgcgtcctg gtagcgcgcc
     2881 ggcaggcgcc gctcggcctc gagcgccgcc tcgcggctag cgaattcgcc gtagacgacg
     2941 cgcagccggt actggccgcc ctcggccatc ggaatcacgg cgagctcatc gaggcgcacc
     3001 tgctcgcggg cacgcagcag gaagcgctcc atgcgcgccg ggtcggtgtt cccggtgatg
     3061 aacagctcga tcgcatagct ctggtcgggt gcgcgcccga gcagctcgcg tgtggcggcg
     3121 aggcgctgcg cgagcag
//
LOCUS       DASYJA010000313         5043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_955454, whole genome shotgun sequence.
ACCESSION   DASYJA010000313 DASYJA010000000
VERSION     DASYJA010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955454"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /gene="glgP"
                     /locus_tag="VGO02_06920"
     CDS             complement(<1..810)
                     /gene="glgP"
                     /locus_tag="VGO02_06920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEV7477452.1"
                     /translation="MQTGTRYFLEVNPRIPQRLARLQELAGNLWYSWDRPTRALFARL
                     HPALWDAVGHSPKAMLKRIDEQRLLDAAADPAFLDSLSRVLAAFDAYHAEPPFRQNSG
                     GFRPGDLVAYFCAEFGVHESLPIYSGGLGILAGDHCKAASDFKLPFVAVGLLYRQGYF
                     VQTIDGEGKQRAEYSDSDFDDLPIEPVRRQDGAELRIEIQFPGRKVLAKVWQARIGHV
                     TLYLLDTGLPENSEHDRAITHRLYGGDRTTRLEQEIVLGVGGVRALEAMGLK"
     gene            929..1336
                     /locus_tag="VGO02_06925"
     CDS             929..1336
                     /locus_tag="VGO02_06925"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7477453.1"
                     /translation="MNTQKNRQLAEELFARFTAGDIPGVLGLMTDDATWRIPGKKGEM
                     PSSGVHSKEKIARVFHAMVGELRGGLKMTLKSAIAEGDRLAVEAESYGELKNGRVYSN
                     EYHFAMTFRDGKISAVREYLDTQHAFLTWFKKE"
     gene            1341..1682
                     /locus_tag="VGO02_06930"
     CDS             1341..1682
                     /locus_tag="VGO02_06930"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV7477454.1"
                     /translation="MELVKDASGLYEVEGRAEHAARPGFRITEIQLSKTQKVPWHYHN
                     NVQDTFYVLAGSIRIFMQQPKEEVRLAPGETYSVPARRPHLVTNGGDTSATFLVLQGL
                     GEYDFVPLLKP"
     gene            complement(1679..2626)
                     /locus_tag="VGO02_06935"
     CDS             complement(1679..2626)
                     /locus_tag="VGO02_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEV7477455.1"
                     /translation="MLLKAEFQSARTSGLPIHRVISAASTIILGKETQVRLALACLLA
                     RGHLLIEDLPGVGKTTLAHVLARSLGMHFQRIQFTSDMLPADILGVSIYDRDTGSFKF
                     HPGPIFSQVILADEVNRSTPKTQSALLEAMEEHQVTAEGETRKLPEPFFVIATQNPSE
                     QVGTFPLPESQLDRFMMRIELGYPDRDAERALLTGIDRRDLLATLDPCMTPAELVELQ
                     AGVQRVHVAPALLDYVQAIVEHTRRSPDYVAGLSPRAALALVHSARAWALLEGRDKVI
                     PEDVQAILPGVAAHRLRPAHEAVRRVDVGAQLLAAVPVP"
     gene            2665..3618
                     /locus_tag="VGO02_06940"
     CDS             2665..3618
                     /locus_tag="VGO02_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEV7477456.1"
                     /translation="MPAAPAASKSVAFVTHADCLKHDMGSHHPERPQRLGSIEEHIAG
                     SDIGAQLVRHEAPLATDEQLARVHPPDYVRAIRDAAPERGTVHLDPDTAMNPFTLKAA
                     LRAAGAATLAVDLVLTKKHSSAFCSVRPPGHHACRARSMGFCIFNNVAVAARHALAEH
                     GLERVAIIDFDVHHGNGTEDIFEGDEQVLMASIFQHPFYPYSGTDDPASNMFNVPLAA
                     GSGGRELRKAVTEIWLPALNAFEPQLICFSAGFDAHVEDDMAMLRFIDQDYAWVTQQV
                     KAIAERHAGGRIVSVLEGGYSLPALGRSAVEHLRVLAGLGG"
     gene            complement(3620..>5043)
                     /locus_tag="VGO02_06945"
     CDS             complement(3620..>5043)
                     /locus_tag="VGO02_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEV7477457.1"
                     /translation="VGGALALAIIASIDALLCAKILARPGEPKVNGDRLLMRLGAANA
                     LAAGAGGITGGLNIGPSRDNRVFGGRTPLSALVNAAVLLVTLVVLFPALSFLPCVAIS
                     AVILVIAVQHFDPWSLRLVRRVASASSSYRWKVLLDLLVVLLVAVLSVAIDIVVAVFV
                     GVAIAALLFMVRMSRSIIRRQYRGTSVRSRKFRPLPDMDALTRTGSAILVMELQGALF
                     FGSAEKVANEIAAQMREDARYVILDLRRITEIDSTGAEILQDINADLTSQGKFLLLSV
                     AQPSAPAELLADFGVADAVTRDRILPDLDRAIAWAEDDLLRTEARPGDEQAEIALDQV
                     GILAGFTPAEVAEVQKHVERVVYEPGRVIFREGDPGNELFIIAKGTASASLRQASGGD
                     IRLVSFAPGTVFGELAMLDAGARSASVATEHELVCYVLTGKDFASLSERSPAVAIKLL
                     ANLARRMSHRLREANRTIQQLEE"
BASE COUNT          841 a         1685 c         1759 g          758 t
ORIGIN      
        1 cttgaggccc atggcttcca gcgcgcgcac gccgccgacg ccgagcacga tctcctgctc
       61 gaggcgcgtc gtgcggtcgc cgccgtacag gcggtgcgtg atggcgcggt cgtgctcgct
      121 gttctccggc aggccggtgt ccaggaggta cagcgtgacg tggccgatgc gggcctgcca
      181 gaccttcgcc aggacttttc tgccggggaa ctggatctcg atgcgcagct cggctccgtc
      241 ctggcggcgc accggctcga tcggcaggtc gtcgaagtcc gaatccgaat actcggcgcg
      301 ctgcttgcct tcgccgtcga tggtctgcac gaagtagccc tggcggtaga gcaggcccac
      361 ggcgacgaac ggcagcttga agtcgctcgc cgccttgcag tggtcgcccg ccaggatgcc
      421 gaggccgccg gagtagatgg gcaggctctc gtgcacgccg aactcggcgc agaagtaggc
      481 caccaggtcg ccggggcgga agccgccgga gttctgccgg aacggcggct cggcgtgata
      541 ggcgtcgaag gccgccagca cccgggagag gctgtcgagg aacgcggggt cggcggcggc
      601 gtcgagcagg cgctgctcgt cgatgcgctt gagcatcgcc ttcgggctgt ggccgaccgc
      661 gtcccacagc gccggatgca gccgggcgaa cagcgcgcgc gtcggccggt cccagctgta
      721 ccagaggttg ccggccagct cctgcaggcg cgcgaggcgc tgcgggatgc gtggattgac
      781 ttcgagaaag tagcgggtcc cggtctgcat gacgttaccg cacgacagct tcctgcttca
      841 ccacttgcgc gcggagcgcg gattctacat aagcaatgtg tcgtggcaac gtgatgcagg
      901 aagcgcgccc gcggcgcaaa ataccaccat gaacacccag aagaacaggc aactggcaga
      961 ggagttgttc gcacgcttca ccgccggaga tattcccgga gtgctcgggc tgatgaccga
     1021 cgacgcgacc tggaggatcc cgggcaagaa gggcgagatg ccgtcctcgg gcgtgcatag
     1081 caaggagaaa atcgcgcgcg tgttccacgc catggtcggc gagctcaggg gtggcctgaa
     1141 gatgacgctg aagagcgcga tcgccgaagg cgaccggctg gcggtcgagg ccgagtcgta
     1201 cggcgagctg aagaacggcc gtgtctacag caacgagtac catttcgcga tgacgttccg
     1261 cgacggcaag atcagcgcgg tgcgcgagta tctcgacacg cagcacgcct tccttacctg
     1321 gttcaagaag gagtagcccg atggaactgg tgaaagacgc aagcggcctg tacgaggtgg
     1381 agggccgcgc cgagcacgcc gcgcggccgg gcttccgcat caccgagatc cagctctcga
     1441 agacgcagaa ggtgccctgg cactaccaca acaacgtgca ggacacgttc tacgtgctgg
     1501 cgggcagcat ccggatcttc atgcagcagc ccaaggagga agtgcggctc gcgccgggcg
     1561 aaacctactc ggtcccggcg cgccggccgc acctcgtcac caacggcggc gacacctcgg
     1621 ccaccttcct cgtgctgcag ggcctgggcg agtacgactt cgtgccgctg ctgaagcctt
     1681 agggaaccgg gacggcggcg aggagctgcg cgccgacgtc gacgcgccgc acggcttcgt
     1741 gcgccgggcg caggcggtgc gcggcgacgc cgggaaggat ggcctgcacg tcctccggga
     1801 tcaccttgtc gcgtccctcg agcagcgccc aggcgcgcgc cgagtgcacc agggcgagcg
     1861 cggcgcgcgg cgacaggccc gcgacgtagt cgggcgagcg gcgcgtgtgc tcgacgatcg
     1921 cctgcacgta atcgagcagg gccggcgcta cgtggacgcg ctgcacgccg gcctgcagct
     1981 ccaccagctc ggccggcgtc atgcagggat cgagcgtggc gagcaggtcg cgccggtcga
     2041 tgccggtgag cagcgcgcgc tcggcgtcgc ggtcggggta gccgagctcg atgcgcatca
     2101 tgaagcggtc gagctgcgac tcgggcagcg ggaaggtgcc gacctgctcc gaagggttct
     2161 gcgtggcgat gacgaagaac ggctcgggca gcttgcgcgt ctcgccctcg gcggtgacct
     2221 ggtgctcctc catcgcctcg agcagcgccg actgcgtctt cggcgtggaa cggttgacct
     2281 cgtcggcgag gatcacctgc gaaaagatcg gccccgggtg gaacttgaag ctgccggtgt
     2341 cgcggtcgta gatcgagacg ccgaggatgt cggcgggcag catgtcgctg gtgaactgga
     2401 tgcgctggaa gtgcatgccg agcgagcgcg ccaggacgtg cgccagcgta gtcttgccca
     2461 cgccgggcag gtcctcgatc agcaggtggc cgcgcgcgag caggcaggcg agcgcgaggc
     2521 gcacctgggt ttccttgccg aggatgatgg tgctggcggc ggaaatgact cgatggatcg
     2581 gcaggcccga ggtgcgcgcc gactggaatt ccgccttgag gagcatgcga cgattgtact
     2641 cgatgcgcta agatggaaat gtccatgccc gccgctccag ccgcgtcaaa atctgtagct
     2701 tttgtcaccc acgccgactg cctgaagcac gacatgggct cgcaccatcc cgagcgcccg
     2761 cagcgcctgg gctcgatcga agagcacatc gccggctcgg atatcggcgc gcagctcgtg
     2821 cgccacgagg cgccgctcgc caccgacgag cagctcgcgc gggtgcatcc gcccgattac
     2881 gtgcgcgcga tccgcgacgc ggccccggaa cggggcaccg tgcacctcga tccggatacg
     2941 gcgatgaacc ccttcacgct gaaggcggcc ttgcgcgcgg cgggcgccgc gacacttgcc
     3001 gtcgatctgg ttctgacaaa aaaacattct tcggcattct gcagcgtgcg cccgcccggc
     3061 caccatgcct gccgcgcccg ttcgatgggc ttttgtattt tcaacaacgt cgccgtcgcc
     3121 gcgcgccacg ccctcgccga gcacgggctg gagcgcgtcg ccatcatcga cttcgacgtg
     3181 caccacggca acggcaccga ggatatcttc gagggcgacg agcaggtgct gatggcctcc
     3241 atcttccagc acccgttcta tccctacagc ggcaccgacg acccggcatc caacatgttc
     3301 aacgtgccgc tcgccgccgg ctccggcggc cgcgagctgc gcaaggcggt gaccgagata
     3361 tggctgcctg ccctgaatgc tttcgagcct caattgattt gtttttccgc cggcttcgat
     3421 gcgcacgtcg aggacgacat ggcgatgctg cgcttcatcg accaggacta cgcgtgggtc
     3481 acgcagcagg tgaaggcgat tgccgagcgc catgccggcg ggcgcatcgt ctcggtcctc
     3541 gaaggcggct actcgcttcc ggcgctcggc cgcagcgcgg tcgagcacct gcgcgtgctc
     3601 gccgggctcg gcggctgacc tactcttcca gctgctggat ggtgcggttc gcctcgcgca
     3661 ggcggtggct catccggcgc gcgaggttgg caagcagctt gatcgcgacc gccggcgagc
     3721 gctcggagag cgaagcgaaa tccttgccgg tcaggacgta gcagacgagc tcgtgctccg
     3781 tggccacgct tgccgagcgt gctccggcgt ccagcatcgc aagctcgccg aagaccgttc
     3841 ccggggcgaa gctcaccagc cggatgtcgc cgccgctcgc ctggcgcagg gacgcgctgg
     3901 cggtgccctt ggcgatgatg aacagctcgt tgccgggatc gccctcgcgg aaaatcaccc
     3961 ggcccggctc gtagacgacg cgctcgacgt gcttttgcac ctcggccacc tccgccgggg
     4021 tgaagccggc caggatgccc acctggtcca gcgcaatctc cgcctgctcg tcgcccgggc
     4081 gcgcctcggt gcgcagcaga tcgtcctccg cccacgcgat ggcgcgatcg aggtccggca
     4141 gaatcctgtc ccgcgtcacc gcatccgcga cgccgaaatc ggccagcagc tcggccggcg
     4201 ccgacggctg cgccacggac aggagcagga acttgccctg cgacgtgagg tcggcgttga
     4261 tgtcctggag gatctcggcg cccgtgctgt cgatctcggt gatgcgccgc agatccagga
     4321 tcacatagcg ggcgtcctcg cgcatctgcg ccgcgatctc gttggcgacc ttctcggccg
     4381 agccgaagaa cagcgcgccc tgcaactcca tgaccaggat ggcggagccg gtgcgcgtga
     4441 gcgcgtccat gtccggcagc ggcctgaact tgcgcgagcg cacgctggtg ccgcggtact
     4501 ggcggcggat gatcgagcgg ctcatgcgca ccatgaaaag cagggcggcg atcgccacgc
     4561 cgacgaatac cgcgaccacg atatcgatcg ccaccgagag gaccgccacc agcaggacca
     4621 ccagcaggtc gagcagcacc ttccagcgat agctcgacga ggccgacgcc acccgccgga
     4681 ccaggcgcag gctccatgga tcgaagtgct ggacggcgat gaccaggatc accgcggaga
     4741 tcgccacgca gggcagaaag ctgagcgccg gaaagagcac caccagcgtg accaggagca
     4801 cggccgcgtt caccagcgct gagagcggcg ttcgtccgcc aaatacccgg ttgtcgcggc
     4861 tgggcccaat gttcaggccg cccgtgatgc cgcctgcacc ggcggccagg gcgtttgcgg
     4921 cgcccaggcg catgagcagg cgatcgccgt tgaccttggg ctcgcccggc cgggccagga
     4981 tcttggcgca aagcagggcg tcgatcgagg cgatgatcgc gagcgcgagc gcgccgccga
     5041 cga
//
LOCUS       DASYJA010000314         3561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_591908, whole genome shotgun sequence.
ACCESSION   DASYJA010000314 DASYJA010000000
VERSION     DASYJA010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591908"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VGO02_06950"
     CDS             <1..927
                     /locus_tag="VGO02_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEV7477458.1"
                     /translation="RYHRIIIMTDADVDGSHIRTLLLTFFYRQMPELVERGHIYIAQP
                     PLYKAKLGKEERYLKDEHELNEYMLRQALVEAVLYPGAGRDPIQGDPLAELAKSYLLA
                     EAVIGRVSRLIDREVLQAILRGVQVDLTRRDTAEASAEALERAIGGNGVAVAPHFDAK
                     TDRYQVRIERSRHGNVRMSTIDTEFVHSGDYTQIRQTAEMLRGLLGAGAYVQRGDKKQ
                     PVSDFSQVMHWLLAEVEKSMSLQRYKGLGEMNPEQLWETTMDPAVRRLLRVQIDDGIA
                     ADEIFIKLMGDEVEPRRAFIESNALGVRNLDV"
     gene            complement(993..3422)
                     /locus_tag="VGO02_06955"
                     /pseudo
     CDS             complement(993..3422)
                     /locus_tag="VGO02_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
BASE COUNT          591 a         1140 c         1118 g          712 t
ORIGIN      
        1 cgctaccacc gcatcatcat catgaccgac gcggatgtcg acggctcgca tatacgaacc
       61 ctgctgctga cgtttttcta ccggcagatg ccggagctcg ttgagcgcgg ccacatctat
      121 atcgcccagc cgccgctcta caaggcgaag ctcggcaagg aagagcgcta tctcaaggac
      181 gagcacgagc tcaacgagta catgctcagg caggccctgg tggaggccgt gttgtatccg
      241 ggtgcggggc gcgaccccat ccaaggcgac ccgctggcgg aactcgccaa gtcgtacctg
      301 ctggccgagg cggtgatcgg gcgtgtctcg aggctgatcg accgcgaggt gctgcaggcg
      361 atcctgcgcg gcgtgcaggt cgacctcacc aggcgcgaca cggccgaggc ttctgccgag
      421 gcgctcgagc gtgcgatcgg cgggaacggt gtcgcggtgg cgccgcactt cgacgccaag
      481 accgatcgct accaggtaag gatcgagcgc tcgcggcatg ggaatgtgcg catgagcacc
      541 atcgacaccg agttcgtgca ttcgggcgac tacacccaga ttcgacagac tgctgaaatg
      601 ctgcgtggct tgctgggcgc aggcgcctat gtgcagcgcg gtgacaagaa gcagccggtc
      661 agcgatttct cacaggtcat gcactggctt cttgccgagg tggaaaaatc catgagcctg
      721 cagcgctaca aaggcctggg ggagatgaac cccgagcagc tctgggaaac caccatggat
      781 ccggcggtgc gccggctgct gagggtgcag atcgacgacg gcatcgccgc cgacgagatc
      841 ttcatcaagc tgatgggcga cgaggtcgag ccgcgccgcg ccttcatcga atccaacgcc
      901 ctgggcgtca ggaacctgga cgtctaggcg gcgcgccgct ttttcttcgg cggcttttcc
      961 ttttcttcgg cggccggctt ctccggcacg ggcttttcct tgcgcggctg gaactcgaag
     1021 ccgaccttgc catccggcgt cttcaccagg aaagccttga aacgccgacc cttctttgaa
     1081 atgaagccgg tgagcagatc ggtgcggccg gtggcgagga gcttcttcat ctgctcgcgc
     1141 tccacgggct gctgcaggat caggcgcccc gagcggaagt cgcaggtgcg cgctggtccg
     1201 agggctttct cgcaggaata cgtgacgccg tgttcgtaga cgcggttacc gcatttcggg
     1261 cacgggccaa gaggctcctg ctggctgaaa tcgggtgctt cctcgtccgc gcccggttgg
     1321 ccgaagtcga actcgggtcg cagctcgtcg ttcagcttca cgccggcagc gaacggcttg
     1381 ccctgcctgc tgcgaaaacc ggtaagcggc ccgacgtaac gcttcttgat gagctcggcg
     1441 atttcatccg acgaccactc acgccccgag accaccttcc agatcgagaa gtcgcacttc
     1501 tggcactgga acttgcggta attctcctgt acttcgccgc cgcatttggg acagggcgcg
     1561 gaaacggtgg cgaacaccgt atccggaatt tcgccgttct tgatgatcgt caccaccttg
     1621 cgcacgagct cggagatgtg atccatgaac tcttcgcgcg aaaatttccc accttcgatg
     1681 agcttgagct tgtgctccca ttctcccgtc agctcgggcg acgcgagctc cggaatgctc
     1741 atcacgtgca gcgcaaacag caacgaaaaa gccttgggtg tgggcaccag ttcgcggccc
     1801 tcgcggtgca catattcctc gcggatcaga ccttcgatgg tcgccgcacg cgtcgccggc
     1861 gtgccgaggc cctttgtccc catcgcctcg cgcatctcct cgtcctcgac cagcttgccc
     1921 gcgccttcca tggctgaaag cagcgtcgcc tcggtataac gcggcggcgg cttggtctgg
     1981 ttggcctgcg aggccacctc cacgagcttc accttctcgt ccttggtgat cgccggcagg
     2041 ttctcgtttt cctcgccacc ggcgcgcccg tagaccgcca gccagcccgg gttctgcaga
     2101 accttgcctt cggtcttgaa ttggtgctcg ccgacgcgcg tgatgcgcgt cgtctgcagc
     2161 gattcggccg gcggatagaa cacggcgaga aaacgccgca ccaccatgtc gtacagcttc
     2221 gcctccgcct cgttcagctg cttgggtacc tgcagagtcg ggatgatcgc aaagtggtcg
     2281 gaaatcttgg tgttgtcgaa gatccgcttg ttcggcttga cccagccttg cttggtgatc
     2341 tgcttggcga aaggcgcgaa ctccttcaag tcggtgagcg attcgagggt ttttttcacc
     2401 gtgccgatat agtcctcggg caacgcgcgg gcgtccgtgc gcggataggt gagcaccttg
     2461 tggcgctcat agagcgcctg ggcaagcgac agggtcatgc gcgcggggaa gccgaaacgc
     2521 ccgttcgcct cgcgctgcag gctggtcagg tcgtagagca gtggcgagag ctgggtggtt
     2581 ggcttggact cctcgctcgc gacgcccgtc ttgccgcggc aggcggcgac gattgcttca
     2641 gcctttgcgg cgctccacat gcgctccggc tttcgctccg cgtcctcgtc cttcttgaac
     2701 ttcgggtcga accaccgccc cgggtactcg cctgccgccg ccccgaacct ggcgtgcacc
     2761 tcccagtagt ccttcggcac gaaggcgcga atcttctcct cccgctcgac catgatggcg
     2821 agagtcgggg tttgtacgcg gcccaccgtc gtcagataga agccgccctc cttggagttg
     2881 aacgccgtca tcgcacgcgt gccgttgatg cccaccagcc agtcggcctc tgaacggcac
     2941 ttggcggcat cggcgagggg cagcatggcc ttgtcctcgc gcaaatcggc gaagccgtcg
     3001 cggatcgatt gctgtgtcat cgactgcagc cacaggcgct cgagcggctt tttggcgcgg
     3061 gcgtgttgca cgatatagcg gaagatcagc tcgccctcgc gcccggcgtc gcaggcgttg
     3121 accaggccgg ttacgtcctt gcgcttcatg aggcgcagca gcacgttcag ccggttctcg
     3181 ctcttctcga tcggcgccag gtcgaaatgc ggcgggatca ccggcaggtg cttgaacgac
     3241 cacttgccct tctttacgtc gtattcctcc ggcaccgcca gtgtgagcag gtggccgacg
     3301 gccgaagaga ggatgtagtc tttcccttcg aagtagtcgc ccttgcgcgt gaagccgccg
     3361 agcgcgcgcg cgatgtcggc ggcgaccgaa ggcttctcgg cgatgattag tttcttggtc
     3421 attgaaagcg aaaggccgca gaggataccc gcaaaggccg cggcggttca acccatcctg
     3481 cgttttgcct acgcgcgcct cagcgctgtt ttgccggcta cgggttgaac cgctcgtgcg
     3541 tttgcactta tcatctgcgg c
//
LOCUS       DASYJA010000315         2281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_682902, whole genome shotgun sequence.
ACCESSION   DASYJA010000315 DASYJA010000000
VERSION     DASYJA010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682902"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VGO02_06960"
     CDS             complement(<1..765)
                     /locus_tag="VGO02_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEV7477459.1"
                     /translation="MKRRSFLKKVAAGAAVGVVAAPAIAQTQPTVTWRMATSWPKSLD
                     TLFGGAELVAKRVGEITDGKFTIRAFAAGEIVPGLAVLDAVQAGTTECGHTAPYYYFG
                     KDPAFAFGTAICFGGNTRQQAAWWLFGGGQEAMAPLYKDYGCISILAGNTNCQMGGWY
                     RKEIKSVADLKGLKMRIGGFAGVILAKLGVVPQQLAAPDIYPALEKGTVDAAEWVGPY
                     DDEKLGFNKVAPYYYTPGFWEGGPMLHVLVNEKKFAE"
     gene            complement(804..1667)
                     /locus_tag="VGO02_06965"
     CDS             complement(804..1667)
                     /locus_tag="VGO02_06965"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV7477460.1"
                     /translation="MTELRPELQVFEKILAEKEERIDLARACLMIAQDAYPSLDVERY
                     LGDIERMAIRLRGRIPQTGQAEERVIALNQFLYGDLGYWGNTEDYYDPRNSYLNEVLD
                     RRTGIPITLGIVYMELGRRVGLPLEGISFPGHFLVRLHLRRGTLVLDPFAGGAPQADD
                     ELRRRLERVVPEHARGGVPLAELPLEQFLEPASKRQILSRVLRNLKGIYREADKPERM
                     LEVLNRMLVVSPEASGELRDRGYLYQRLECWRPALKDLTDYLERVPDAADVDDVRASL
                     LDLSARCARLN"
     gene            complement(1668..>2281)
                     /locus_tag="VGO02_06970"
     CDS             complement(1668..>2281)
                     /locus_tag="VGO02_06970"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEV7477461.1"
                     /translation="GLRITVLLAVPAAAALAVLALPLVATLFQYGRFTVGDAWMTQRA
                     LVAYSIGLIGMILVKILAPGFYARQNIVTPVKIGILTLVVTQAMNFAFIGPLQHAGLA
                     LAIGLGACLNAFLLFFLLRKQQVYLPQPGWLGFTLKVAAAVALMCAVLYFAMGGAPWW
                     LDNSWQRKVPAVMGLVVLGAAVYAACLAALGLRPRDFSRRGAA"
BASE COUNT          387 a          766 c          768 g          360 t
ORIGIN      
        1 ctcggcaaat ttcttctcgt tgacgagcac gtgcagcatc gggccgccct cccagaagcc
       61 gggggtgtag taatacggcg ccaccttgtt gaagccgagc ttctcgtcgt cgtagggacc
      121 gacccactcg gccgcatcca cggtgccttt ttcgagcgcg gggtagatgt cgggcgcggc
      181 cagctgctgc ggcacgacgc cgagcttggc caggatgaca ccggcaaagc cgccgatccg
      241 catcttcagg cctttcaggt cggcgaccga cttgatctcc ttgcggtacc agccgcccat
      301 ctggcagttg gtgttgccgg cgaggatgga aatgcagccg tagtccttgt acagcggggc
      361 catcgcttcc tgaccgccgc cgaacagcca ccacgccgcc tgctggcgcg tattgccgcc
      421 gaagcagatg gcggtgccaa aggcgaatgc cgggtccttg ccgaagtagt agtacggcgc
      481 ggtgtgcccg cactccgtgg tgccggcctg cactgcgtcg aggaccgcca gtcccggcac
      541 gatctcgcct gcggcaaacg cgcggatggt gaactttccg tcggtgatct cgccgacgcg
      601 cttcgccacc agctcggcgc cgccgaacag cgtatccagg ctcttcggcc agctggtggc
      661 catgcgccag gtgacggttg gctgcgtctg cgcgatcgcc ggcgcggcga cgacccccac
      721 agccgcgcca gcggccacct tcttcagaaa cgatctgcgt ttcatgctgc tccctcctca
      781 caaggaaagc agcgattcta gtctcaattg agtcgagcac agcgcgccga gaggtcgagg
      841 agcgaggcgc gcacgtcgtc gacgtcggcg gcatcgggca cgcgctccag atagtccgtc
      901 aggtccttga gcgccgggcg ccagcactcg aggcgctggt agaggtagcc gcggtcgcgc
      961 agctcgccgc tcgcctccgg cgaaaccacc agcatgcggt tgagcacctc gagcatccgc
     1021 tcgggcttgt cggcctcgcg gtagatgcct ttaaggttgc gcagcacgcg cgacaggatc
     1081 tgccgcttcg atgccggctc gaggaactgc tcgagcggca gctcggccag cggcacgccg
     1141 ccgcgcgcgt gctcgggcac cacgcgctcc aggcgccggc gcagctcgtc atcggcctgc
     1201 ggcgcgccgc cggcgaacgg gtcgagcacc agcgtgccgc ggcgcaggtg caggcgcacc
     1261 aggaaatgcc ccgggaagga gatgccctcg agcggcagcc cgacgcggcg gccgagctcc
     1321 atgtagacaa tgcccagggt gatcgggatg ccggtcctgc gatcgagcac ctcgttgaga
     1381 tagctgttgc gcgggtcgta gtagtcctcg gtgttgcccc aatagccgag gtcgccgtag
     1441 aggaactggt tgagcgcgat cacgcgctcc tcggcctggc cggtctgcgg gatgcgcccg
     1501 cgcaggcgga tcgccatgcg ctcgatgtcg cccaggtagc gctcgacgtc gaggctcggg
     1561 taggcgtcct gcgcgatcat caggcaggcg cgcgcaaggt ctattctttc ctctttctcg
     1621 gcgagaattt tttcgaaaac ctgaagctcg ggccgcagtt cggtcattta tgcggcgccc
     1681 cggcgcgaga agtcgcgcgg acgcaagccg agcgcggcga ggcaggcggc gtagacagcc
     1741 gccccgagga cgaccaatcc catcacggcg ggaaccttac gctgccagct gttatcgagc
     1801 caccatggcg cgccgcccat tgcgaaatac agcacggcgc acatgagcgc cacggcggcg
     1861 gcgaccttga gcgtgaagcc gagccagccg ggctgcggca ggtagacctg ctgttttctc
     1921 aacaggaaaa acagcagaaa ggcattcagg caggcgccca gcccgatcgc cagcgcgagt
     1981 ccggcgtgct gcagcgggcc gatgaaggcg aagttcatcg cctgcgtgac caccagggtc
     2041 aggatgccga tcttcaccgg ggtgacgatg ttctgcctgg cgtagaagcc cggcgcaagg
     2101 atcttcacca ggatcatgcc gatcaggccg atgctgtagg ccaccagcgc gcgttgcgtc
     2161 atccacgcgt ccccgacggt gaagcggccg tactggaaaa gggtggcgac cagcggcagc
     2221 gcgagcaccg ccagcgccgc cgcagccggc acggcgagca gcaccgtgat acgcaggccc
     2281 c
//
LOCUS       DASYJA010000316         4219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_955551, whole genome shotgun sequence.
ACCESSION   DASYJA010000316 DASYJA010000000
VERSION     DASYJA010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955551"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VGO02_06975"
     CDS             complement(<1..905)
                     /locus_tag="VGO02_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEV7477462.1"
                     /translation="MKVSVVGGGPGGLYFALLAKKAWPRWDITVYERNRPDDTFGFGV
                     VFSDQTLDAFKAYDTVTYERIRRRFAYWGDVDVVYKGKVMRSGGNGFCGCSRNALLNI
                     LQDRGRELGVQLRFQREVEGLDQFADSDLVVVADGINSRIREKYKDHFQPSVDLRPNK
                     FTWLGSTKPLDAFKYFFRETKEGLIVAHCYQYEPDRSTWVIEADEQTWKNFGFDKLSE
                     TDMLPVIEGVFAEELDGHRLIANRSLWRNFPTITNKTWVKGNAVLVGDAKATAHFSIG
                     SGTKLAMEDAIALFEAFRSEESVGQA"
     gene            complement(902..1294)
                     /locus_tag="VGO02_06980"
     CDS             complement(902..1294)
                     /locus_tag="VGO02_06980"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV7477463.1"
                     /translation="MQKILQPPGWARAKGFSNGIACSGNLVFIAGQIGWTGQGKWEAK
                     DFAGQFRQALQNIIDVLKEAKGKPEHIVRLTWYVVDKQEYLNSLKNVGEAYRELMGRH
                     YPTMAVVQVSGLMEDEARLEIEATAVLP"
     gene            complement(1297..2907)
                     /locus_tag="VGO02_06985"
     CDS             complement(1297..2907)
                     /locus_tag="VGO02_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7477464.1"
                     /translation="MSYTAHLDSFARDNLPPRALWPELRFDLPELQYPSRLNCAAELL
                     DKPVTRGHGHRVALRSADGECTYTQLFTQANRLANVLVREMGLKPGNRVLLRGPNNPI
                     MAACWFAVMKAGGVCVATMPLLRAKELTEIIEKAEVSHALCDRRLDEELKKVNSSFLK
                     QIKYWYDDTADSLDTLALRQPLWFTNVPTAADDVALIAFTSGTTGKPKGTMHLHRDVI
                     AMCDLFPRSCLGLHKDDVVCGTPPLAFTFGLGGMLCFPMRYGASSVLVEKHTPQTLLE
                     TIEGFKATVCFTTPIMYRQMSLLAKDHDLKSLRACVSAGEALPDATRRAFREASGIEI
                     IDGIGSTEMIHIFISHTPQRVRRGATGYAIPGYQATVLNNELLPCKPGEVGRLAVKGP
                     TGCRYLADERQKNYVQRGWNLTGDAYTMDADGYFFFQARTDDLIISAGYNIAGPEVED
                     ALLIHPAVAECGVVGEPDAERGTIVKAFVVLKKDFPKDDSMVKALQEHVKNAIAPYKY
                     PRAIEFIDALPRTETGKVQRFKLRGLDK"
     gene            complement(2904..4010)
                     /locus_tag="VGO02_06990"
     CDS             complement(2904..4010)
                     /locus_tag="VGO02_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7477465.1"
                     /translation="MDRFAHLDWPFFEDQHRKLARAADAWAAANLGHSHGEDADAICR
                     RLVQDLGCAGYLRHSATEKPDVRSIALLREVFAYHSGLADFSFVMQGLGSGPIALSGS
                     EEQKNSYLKKAAAGDAIAAFALSEPDAGSDVQALTTTARREGKGWVLNGTKTWISNGG
                     IADFYVVFARADEGISAFVVDAKDVDASERIDIVAPHPMAVVKLKNSKGSLLGEPGQG
                     FKLAMRNLDIFRTTVAAAAIGFARRALAESLQRAKDRKMFGQTLGDLQMSQAKLADMA
                     TSIDAAALLTYRSAWKKDVKGERVTREAAMAKMFATEAAQAVVDDAVQLLGGLGVKRG
                     HPVERLYREVRALRIYEGATEVQKLIIAKDLLKG"
     gene            complement(4010..4219)
                     /locus_tag="VGO02_06995"
                     /pseudo
     CDS             complement(4010..4219)
                     /locus_tag="VGO02_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092719.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
BASE COUNT          689 a         1368 c         1333 g          829 t
ORIGIN      
        1 gcttgcccta cggactcttc cgagcggaaa gcttcaaaca gggcgatggc gtcttccatc
       61 gccagcttgg tgcccgagcc gatggagaag tgcgcggtgg ccttggcgtc gcccaccagc
      121 acggcgttgc ccttcaccca cgtcttgttc gtgatggtgg ggaagttgcg ccacagcgag
      181 cggttggcga tcagccggtg gccgtcgagc tcctcggcaa agacgccctc gatcaccggc
      241 agcatgtcgg tctcggagag cttgtcgaag ccgaagttct tccaggtctg ctcgtcggcc
      301 tcgatcaccc aggtcgagcg gtcgggctcg tactggtagc agtgcgcgac gatcaatcct
      361 tccttggttt ccctgaaaaa atatttgaac gcgtccagcg gcttggtcga gcccagccag
      421 gtgaacttgt tgggccgcag gtcgacgctc ggctggaaat ggtccttgta tttctcacgg
      481 atgcgcgaat tgatgccgtc ggcgaccacc accaggtcgg agtctgcgaa ctggtcgagg
      541 ccctcgacct cgcgctggaa gcgcagttgc acgccgagtt cgcggccgcg gtcctgcagg
      601 atgttgagca gcgcgttgcg cgagcagccg cagaagccgt tgccgcccga gcgcatcacc
      661 ttgcccttgt agaccacgtc gacgtcgccc cagtaggcga agcgccggcg gatgcgctcg
      721 taggtgacgg tgtcgtaggc cttgaaggcg tcgagcgtct ggtcggaaaa caccacgccg
      781 aagccgaagg tgtcgtcggg ccggttgcgc tcgtagaccg tgatgtccca gcgcggccag
      841 gccttcttcg ccagcagcgc gaagtacagg ccgcccggcc cgcctcccac cacgctcacc
      901 ttcatggcag caccgccgtc gcttcgatct cgaggcgcgc ctcgtcctcc atcaggccgc
      961 tcacctgcac caccgccatc gtcgggtagt gcctgcccat cagctcccga tacgcctcac
     1021 caacgttttt caatgaattc aaatactcct gcttgtcgac gacgtaccag gtgaggcgca
     1081 cgatgtgctc gggttttcct tttgcttcct tgaggacgtc gatgatgttc tgcagagcct
     1141 ggcggaattg ccccgcgaag tccttcgcct cccatttccc ctggccggtc cagccgatct
     1201 ggccggcgat gaagaccagg ttccccgagc aggcgatgcc gttggagaac cccttggcgc
     1261 gcgcccagcc cggcggctgg aggattttct gaatgctcat ttgtccaaac ctcttagttt
     1321 gaagcgctgc actttcccgg tctcggtgcg cggcagcgcg tcgatgaatt cgatcgcccg
     1381 cggatacttg tagggtgcga tggcgttctt gacgtgctcc tgcagtgctt tcaccatcga
     1441 atcatccttg ggaaaatctt tttttaagac cacaaaggct ttgacgatcg tgccgcgctc
     1501 cgcgtcgggc tcgccgacca cgccgcattc ggccaccgcc gggtggatca gaagcgcgtc
     1561 ctcgacctcc ggcccggcga tgttgtagcc cgccgagatg atcaggtcgt cggtgcgcgc
     1621 ctggaagaag aagtagccgt cggcgtccat cgtgtaggcg tcgccggtca ggttccagcc
     1681 gcgctgcacg tagtttttct gccgctcgtc ggcgaggtag cggcagccgg tcgggccttt
     1741 gacggcgagc ctgccgactt caccgggctt gcaaggaagc aattcgttgt tgagaaccgt
     1801 ggcctgatag ccggggatgg catagccggt cgcgccccgg cgcacgcgct gcggcgtgtg
     1861 cgagatgaag atgtggatca tctcggtcga gccgatgccg tcgatgatct cgatgccgct
     1921 cgcctcgcgg aaggcgcgcc gcgtggcgtc gggcaacgct tcgccggccg aaacgcaggc
     1981 tcgcaggctt ttcagatcat gatcttttgc gagcaaagac atctggcgat acatgatcgg
     2041 cgtcgtgaag cacacggtcg ccttgaaccc ctcgatggtt tccagcaagg tctgcggcgt
     2101 gtgcttctcg accagcacgc tggaagcgcc gtagcgcatc gggaagcaca gcatgccgcc
     2161 caggccgaag gtgaaggcaa gcggcggcgt gccgcagacg acatcgtcct tgtgcaggcc
     2221 gaggcaggag cgcgggaaca ggtcgcacat cgcgatcacg tcgcgatgca gatgcatcgt
     2281 gcccttgggc ttgccggtgg tgcccgaggt gaaggcgatc agcgccacgt cgtcagccgc
     2341 ggtgggcacg ttggtgaacc acagcggctg cctgagcgcg agcgtatcga gcgagtcggc
     2401 tgtgtcgtcg taccagtact tgatttgttt caggaacgaa gaattcactt tcttcagttc
     2461 ctcgtcgagc cgccgatcgc acagcgcgtg cgagacctcg gctttctcga tgatctcggt
     2521 cagctccttc gcgcgcagca gcggcatggt ggcgacgcat acgccgcccg ccttcatcac
     2581 cgcgaaccag cacgccgcca tgatcgggtt gttcggcccg cgcagcagga cgcggttgcc
     2641 gggcttgagc cccatctcgc gcaccagcac gttggcgagg cggttggcct gcgtgaagag
     2701 ctgggtgtag gtgcactcgc cgtcggccga gcgcagcgcg acgcggtggc cgtggccgcg
     2761 cgtcaccggc ttgtcgagga gctcggcggc gcagttcagg cggctcgggt actgcagctc
     2821 gggcaggtcg aagcgcagct ccggccacag cgcgcgcggc ggcagattgt cacgcgcgaa
     2881 gctatccaga tgggctgtgt aactcatcct ttcagaaggt ctttcgcaat gatcaatttc
     2941 tgtacctcgg ttgccccctc gtatatgcgc agcgcacgca cctcgcggta gagccgctcg
     3001 accgggtgcc cgcgcttcac gcccaggccg ccgaggagct gcaccgcgtc gtcgaccacc
     3061 gcctgcgccg cctcggtggc aaacatcttc gccatcgccg cctcgcgggt gacgcgctcg
     3121 cctttcacgt ccttcttcca ggcggaacgg taggtgagca gcgccgccgc gtcgatggac
     3181 gtcgccatgt cggcgagctt ggcctgcgac atctgcagat cgccgagcgt ctggccgaac
     3241 atctttctgt cttttgccct ttgcagcgac tccgccagag cccgtcgggc aaaaccgatc
     3301 gcggcagccg cgacggtagt cctgaagata tcaagattcc tcatcgccag cttgaagccc
     3361 tgccccggct cccccagcaa actgcctttg ctgttcttca gcttgactac tgccatcgga
     3421 tggggcgcca cgatgtcgat gcgctcggag gcgtcgacgt ccttcgcgtc gacgacgaag
     3481 gccgagatgc cctcgtctgc cctggcgaac acgacataga aatccgcgat gccgccattg
     3541 gagatccagg tcttggtgcc attgagcacc cagcccttgc cctcgcgcct tgccgtggtc
     3601 gtcaatgcct ggacgtcgga accggcgtcg ggctcggaca gggcgaacgc ggcgattgca
     3661 tcgcccgctg cggctttctt cagatacgaa ttcttctgct cttcacttcc agaaagagcg
     3721 atcggaccgc tgcccagtcc ctgcatgaca aacgaaaagt cggccaggcc cgagtggtac
     3781 gcgaacacct cgcgcaacag cgcgatcgag cgcacgtcgg gcttctcggt ggcgctgtgg
     3841 cggaggtatc cggcgcaacc caggtcctgc accaggcgcc tgcagatcgc gtcggcgtcc
     3901 tcgccgtggc tgtggccgag gttcgccgcc gcccaggcgt cggccgcgcg cgccagtttt
     3961 cgatgctggt cctcgaaaaa aggccagtcc aggtgggcaa agcgatccat cagtctcctt
     4021 cgaagaccgg cttgcgcttc gccgcaaacg cctcgtaggc gcggtggaag tccttggtct
     4081 gcatgagaat ggcctgggcc tgcgcttcca tttcgatcgc ctcgtcgagg ccggcgttcc
     4141 attcctagtg cagcattttc ttggtcatgg cgtgcgccag catcggaccc gcagcgaggt
     4201 cttgcgcaat ttcaattgc
//
LOCUS       DASYJA010000317         1797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_137363, whole genome shotgun sequence.
ACCESSION   DASYJA010000317 DASYJA010000000
VERSION     DASYJA010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137363"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VGO02_07000"
     CDS             complement(<1..548)
                     /locus_tag="VGO02_07000"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEV7477466.1"
                     /translation="MATHELHSSPETCHWGYFDCQLKPSLRIKSGDIATIHCVSGSAE
                     ILPGEPFNVLPEHREILGTLKPHLGRHILTGPVYVEGAERGDALAVEVLDIKFRTNWG
                     WNAQRPLAGTLPEDFPFYRLTHIPIDSNRGVATLPWGLEIELKPFFGIMGVAPPPEWG
                     QCSSIEPRAFGGNIDNKHFNIGT"
     gene            625..1488
                     /gene="rlmJ"
                     /locus_tag="VGO02_07005"
     CDS             625..1488
                     /gene="rlmJ"
                     /locus_tag="VGO02_07005"
                     /inference="COORDINATES: protein motif:HMM:NF016278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEV7477467.1"
                     /translation="MNYRHGYHAGNFADVLKHTALCELLRLLTAKDKKLFVLDTHAGA
                     GGYDLASGPARRTGEAEAGIARLVGSPRAEMPAAVVRYLAAVAAYDRKFGAAAGGIRR
                     YPGSPRLVRAALRPGDRFIACELHPAEALALKREFAGDRAVEGRQADGYKVLKALLPP
                     LERRGLVLIDPPFEAADEFERLLRALRQGVRRFATGCYALWYPIKDEAAAAFAGELAA
                     FKPLILELHSDAVEPGKLAACGLAVINPPWRFEEAMREALPWIAERLGPGVRLAISAP
                     PASAPAPASAP"
     gene            complement(1430..>1797)
                     /locus_tag="VGO02_07010"
     CDS             complement(1430..>1797)
                     /locus_tag="VGO02_07010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7477468.1"
                     /translation="ALMYIGAGAMLVMWSQSTSAPALAFFALLFGAFYGGFVAIAPSL
                     AADYFGGRALGSIIGALYSGVAFGALLGAPAAGYAFDLFGSYTGVTLAGAALCLVSFV
                     ITVLMPEPARWLAGRKSPT"
BASE COUNT          286 a          589 c          654 g          268 t
ORIGIN      
        1 gtgccgatat tgaagtgctt gttgtcgatg ttgccgccga aggcgcgcgg ctcgatcgag
       61 ctgcactggc cccattccgg cggcggcgcc acgcccataa tgccgaagaa cggcttgagc
      121 tcgatctcga ggccccacgg cagggtagcg acgccgcggt tggaatcgat ggggatgtgg
      181 gtcaggcgat agaacgggaa gtcctcgggc agagtgccgg ccagcggccg ctgggcgttc
      241 cagccccagt tggtgcggaa tttgatgtcg agcacctcga ccgccagcgc atcgccgcgc
      301 tcggcgcctt cgacataaac cgggccggtc aggatgtggc ggccgagatg cggcttcaat
      361 gtgcccagga tctcgcgatg ctcgggcagc acgttgaacg gctcgccggg caggatttcg
      421 gccgagcccg aaacgcaatg gatggtggcg atgtcaccgg atttgatgcg cagcgatggc
      481 ttcagctggc agtcgaaata gccccagtgg caggtctcgg gcgaagaatg aagttcgtgc
      541 gtggccatgg ccgtccccct ttgtgccccc gggttgaagc acataccgtg cccccaaatc
      601 catggctgat gctataggcg tggcatgaac tatcgccatg gctatcacgc cggcaacttc
      661 gccgatgtcc tgaagcacac ggcgctctgc gaactgctgc gcctgctgac ggccaaggac
      721 aagaagctgt tcgtgctcga cacccatgcc ggcgccggcg gctacgatct ggcgagcggg
      781 ccggcgcggc gcaccggcga ggccgaggcc ggcatcgccc gcctggtcgg ctcgccgcgc
      841 gctgaaatgc cagccgccgt ggtgcgctat ctggcggcgg tcgcggccta cgaccgcaag
      901 ttcggtgccg ccgcgggtgg tatccggcgc tatcccggtt cgccgcggct ggttcgcgcg
      961 gcgctgcggc cgggcgatcg cttcatcgcc tgcgaactgc acccggcgga agcgctggcc
     1021 ctcaagcgcg agttcgccgg cgatcgcgcg gtcgaggggc gccaggccga cggctacaag
     1081 gtgctgaagg cgctgttgcc gccgctcgag cgccgcggcc tggtgctgat cgatccgccc
     1141 ttcgaggcgg ccgacgagtt cgagcgcctg ctgcgcgccc tgcgccaggg cgtgcgccgc
     1201 ttcgccaccg gctgctacgc gctctggtat ccgatcaagg acgaggccgc ggccgcgttc
     1261 gccggcgagt tggcggcttt caagccgctc atcctggaac tgcactcgga tgcggtcgag
     1321 ccgggcaagc tcgcggcgtg cgggctggcg gtcatcaatc cgccctggcg tttcgaggag
     1381 gcgatgcgcg aagcgctgcc gtggatcgcc gagcgcctgg ggccgggcgt caggttggcg
     1441 atttccgccc cgccagccag cgcgccggct ccggcatcag caccgtgatg acgaacgaca
     1501 ccaggcagag cgcggcgccc gccagcgtga cgccggtgta ggaaccgaag aggtcgaaag
     1561 cgtagcccgc ggccggcgcg ccgagcaggg cgccgaacgc cacgccgctg tagagcgcgc
     1621 cgatgatcga gccgagcgcg cggccgccga aatagtcggc ggcgagcgag ggcgcgatgg
     1681 cgacgaagcc gccgtagaag gcgccgaaca gcagggcgaa gaaggcgagg gcaggcgcgc
     1741 tggtcgattg cgaccacatc accagcatgg cgccggcgcc gatatacatc agggcga
//
LOCUS       DASYJA010000318         1736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2316773, whole genome shotgun sequence.
ACCESSION   DASYJA010000318 DASYJA010000000
VERSION     DASYJA010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2316773"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..873)
                     /gene="grpE"
                     /locus_tag="VGO02_07015"
     CDS             complement(109..873)
                     /gene="grpE"
                     /locus_tag="VGO02_07015"
                     /inference="COORDINATES: protein motif:HMM:NF010739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV7477469.1"
                     /translation="MTDSTSGRPDDITSMASEPLPGADSAASEAERVAALVEQLAREA
                     SENKDKLLRSLAEMENMRRRTDRQVADAREYGITAFARDVLAVADNMNRALSAIDPQL
                     RETADAGLKSLLEGVELTERELLNVLEKHGVKRFEPTGEKFDPNLHQAMYEVPDSSLP
                     NGSVAKVVQAGYMIGDRVLRPALVGVAKARPKPPPAPPPQELAAKAEEPAEGLADEHV
                     ETSANDNTGEPAPDIVDEQDILDIPNPLRSPKSRPT"
     gene            complement(989..1306)
                     /locus_tag="VGO02_07020"
     CDS             complement(989..1306)
                     /locus_tag="VGO02_07020"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEV7477470.1"
                     /translation="MRALAIANEERLAEFPDLPIMAETYPGFVATSWFALLAPPKTPA
                     AIVDKLWQAVAEALRMPDVEARLRGIGAAPGGSTPAATAAFLRQETERWSKVIAQANI
                     KPE"
     gene            complement(1454..>1736)
                     /locus_tag="VGO02_07025"
     CDS             complement(1454..>1736)
                     /locus_tag="VGO02_07025"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEV7477471.1"
                     /translation="DFPAIPALGEVYSGVVHEDWFVVVAPPRTPPQIANKLSQAIAET
                     LMLPDVAKRVSDAGFTSVGSWPAQTAAMIKAESEKFRPIIAEAGIKIEQ"
BASE COUNT          281 a          594 c          548 g          313 t
ORIGIN      
        1 aagggccaag cctaaacgca atctgacggc gatgtatcac gactttggag gcgtggcagc
       61 agggtcctgc catccaaaca ggcagcagga tgggctcgtc cccttcgctc aggtgggtcg
      121 cgacttcggc gaacgcagcg ggttcggaat gtctaaaatg tcctgctcgt ccacgatgtc
      181 cggcgcaggc tcgccggtat tgtcgttcgc agacgtctcg acgtgctcgt ccgccagccc
      241 ctcggccggt tcttcggcct tggccgcaag ctcctgcgga ggcggggcgg gcggcggctt
      301 gggcctggcc ttggccaccc cgaccagcgc cgggcgcagc acccggtcgc cgatcatgta
      361 gccggcctgc accaccttgg cgacgctgcc gttcggcagc gaagaatcag gcacctcata
      421 catggcctga tgcaggttcg gatcgaactt ctcgccggtc ggctcgaacc tcttaacgcc
      481 gtgcttctct agaacgttga gcagctcgcg ctcggtcagc tcaacgccct cgagcagaga
      541 cttgaggccc gcatccgccg tctcacgcag ctgcggatcg atcgccgaca gcgcgcggtt
      601 catgttgtcg gcaaccgcga gcacgtcgcg cgcgaaagcc gtgatgccat attcgcgcgc
      661 gtccgcgacc tgccggtcgg ttcgacggcg catgttctcc atctcggcga gcgagcgcag
      721 cagcttgtcc ttgttctcgg acgcttcacg cgccagctgc tcgaccagcg ccgccacccg
      781 ctcggcttcg ctcgccgcac tgtccgcgcc cggcagcggc tcagaagcca tcgacgtgat
      841 gtcgtcggga cggccagagg tcgaatccgt catcgtgtgg ttcccgccac aatttgagtg
      901 ctgtctgccg ggatatcagg gctctgcgga caaaaatcaa gctcgtatcg cgccttgctc
      961 gaaaagcctg cacaatgcgg gcctcgcctc attccggctt gatgttggcc tgcgcgatga
     1021 ccttgctcca gcgctcggtc tcctgccgca ggaatgccgc ggttgcggcc ggtgtcgatc
     1081 caccaggcgc agcaccgatg ccgcgcaggc gggcctcgac atccggcatc cgcagcgcct
     1141 cggccacggc ctgccagagc ttgtccacga tggcggccgg cgtcttcggc ggagccagca
     1201 gcgcgaacca gctggtagcc acgaagcccg ggtaggtctc cgccatgatt ggcaggtcgg
     1261 gaaactcggc gagccgctct tcgttggcaa tcgcaagcgc ccgcaacctc ccggcgcgcc
     1321 cggcgcgagc ggcaccacga tcttgatcgt gcggttggga tagtcgccct gcgcgagcgc
     1381 cgaagcaggc gcaagccacg cgagcagcag gagggcccaa attcggccgg cgtcactcat
     1441 tgttcgattt ggctcattgc tcgatcttga tgccggcttc cgcgatgatc ggacggaatt
     1501 tctcgctctc cgccttgatc atcgcggcgg tctgggccgg ccacgagccg accgacgtga
     1561 agcccgcatc gctgacgcgc ttggccacat cgggcagcat gagcgtctcg gcgatcgctt
     1621 gcgagagctt gttggcaatc tgcggcggcg ttctcggtgg cgccaccacc acgaaccagt
     1681 cctcgtgcac cacaccagaa tagacttcgc cgagcgccgg aatcgctgga aaatca
//
LOCUS       DASYJA010000319         1812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_774790, whole genome shotgun sequence.
ACCESSION   DASYJA010000319 DASYJA010000000
VERSION     DASYJA010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774790"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VGO02_07030"
     CDS             <1..808
                     /locus_tag="VGO02_07030"
                     /inference="COORDINATES: protein motif:HMM:NF019272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEV7477472.1"
                     /translation="WTKKNPMALASHHVAFTEYKGKIYAFGGFVLPQSGPPAWVPIDN
                     AWEYDPAKDTWRALAPLPTKRGSPIAVTVGDKLYVMGGATMLAGAAAVHPARPHASVA
                     AVEEYDPATNTWRARSPMPTPRNHAVAGVVNGKVYVIGGRVGGAFITSGSSNASVVEE
                     YDPASDSWGGPKARMPTARSAMAAGVYSNRIYVTGGEFQDASMMATFRSFESYDPATN
                     TWSTLPSMPTSRHGLASAVLGNRLHMVSGDTQSALSGAHGQTEAHDAYEF"
     gene            870..1190
                     /locus_tag="VGO02_07035"
     CDS             870..1190
                     /locus_tag="VGO02_07035"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV7477473.1"
                     /translation="MNQIMGGPAYSPVFGGCVEGERMIVALMRYPAGKPSDAHSHPNE
                     QWIFILEGELEMEFDGKWHKGKVGDVFYVPAGKVHKARNESESDCVFFTCKDASHGLH
                     GTKA"
     gene            1215..1466
                     /locus_tag="VGO02_07040"
     CDS             1215..1466
                     /locus_tag="VGO02_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477474.1"
                     /translation="MANPRIAGIFEDPIAAERAKERLLEAGVALHRIVVSAPANDSIA
                     PQWRSATRVVTVAARSEVEKERIARLMLSSGARDTVTPP"
     gene            complement(1467..1676)
                     /locus_tag="VGO02_07045"
     CDS             complement(1467..1676)
                     /locus_tag="VGO02_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477475.1"
                     /translation="MEDPRPVRAQALRRAAEILGGRSPLRAYLNVSALVLGMWMSGAQ
                     PPPTDVFLKVVDLIIEKDLDSLRRH"
BASE COUNT          354 a          594 c          609 g          255 t
ORIGIN      
        1 gtggacgaag aagaatccaa tggcgctcgc ctcgcaccac gttgccttca ccgagtacaa
       61 gggaaagatc tacgccttcg gcggcttcgt cctgccgcaa tcgggcccgc cggcctgggt
      121 gccgatcgac aacgcctggg aatacgatcc ggcgaaagac acctggcgcg cgctcgcgcc
      181 gctgcccacc aagcgcggct cgccgatcgc ggtgacggtg ggcgacaagc tgtacgtgat
      241 gggcggcgcc accatgctgg ccggcgcggc ggcggtgcac ccggcgcggc cgcacgcttc
      301 ggtggcggcg gtcgaggagt acgaccccgc gaccaacacc tggcgagcgc gctcgccgat
      361 gccgacgccg cgcaaccatg ccgtcgccgg cgtggtcaac ggcaaggtct acgtcatcgg
      421 cgggcgcgtc ggcggcgcct tcattacgag cggcagcagc aacgcgtcag tggtggagga
      481 atacgacccc gcgagcgaca gctggggcgg accgaaagcg cgcatgccga cagcgcgcag
      541 cgcgatggcc gccggggtct acagcaacag gatctatgtc acgggcggcg aattccagga
      601 tgccagcatg atggcgacct tccgctcctt cgagtcctac gacccggcga cgaacacctg
      661 gagcacgctg ccgtcgatgc ccacctcgcg ccacggcctg gcgagcgcgg tgctcggcaa
      721 ccgcctgcac atggtgagcg gcgacacgca atccgcgctc agcggcgcgc acggccagac
      781 agaagcgcac gacgcctatg agttctgaag ccaggacccc gggagcgaaa agggccgcac
      841 ccggcgaaca tgtattcgat ctgaataacg tgaaccagat catgggcggc ccggcgtact
      901 cgccggtatt cggcggctgc gtggaaggcg agcgcatgat cgtggcgctg atgcgctatc
      961 ccgccggcaa gccctccgac gcgcattcgc acccgaacga gcagtggatc ttcatcctcg
     1021 agggcgagct cgagatggag ttcgacggca aatggcacaa aggcaaggtg ggagacgtct
     1081 tctacgttcc tgccggcaaa gtccacaagg cgcgcaacga gagcgagtcc gactgcgtgt
     1141 tcttcacctg caaggacgcc tcgcacggcc tgcacggtac gaaggcctag gcacgagggt
     1201 tgcggggggc cggcatggcc aaccccagga ttgcaggcat cttcgaggat cccatcgccg
     1261 ccgagcgcgc gaaggaaagg ctgctcgagg ccggcgtcgc gctgcaccgg atcgtggtga
     1321 gcgcgccggc caacgacagc atcgccccgc agtggcgcag cgcaacccgc gtggtcaccg
     1381 tcgccgcgcg ctccgaggtg gaaaaggagc gcattgcgag gctcatgctc agcagcggcg
     1441 cacgcgacac cgtcacaccg ccgtagtcag tgcctgcgca ggctgtcgag atccttctcg
     1501 atgatcaggt cgaccacttt gaggaagaca tctgtcggcg gaggttgcgc accggacatc
     1561 cacatgccca gcacaagggc tgagacgttc aggtaagcgc gcagcgggct gcgtccaccg
     1621 aggatttccg ccgcgcgccg caaagcctgg gcgcgtaccg gtctcggatc ttccatccgg
     1681 cgagactagc agaatgaact gacgatagcc tgaagccgag cggacggaat gattattgct
     1741 gctccggcgt accaacccgg aggctcaatg gaaaccaagc aattgaagga gttactgctg
     1801 cagtcgctcg ag
//
LOCUS       DASYJA010000320         1762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2044680, whole genome shotgun sequence.
ACCESSION   DASYJA010000320 DASYJA010000000
VERSION     DASYJA010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2044680"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="VGO02_07050"
     CDS             <1..1135
                     /locus_tag="VGO02_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477476.1"
                     /translation="INGNLRLGFINQNDIGDPDNYSAKNIVVRNISFADSFDMFPSWD
                     PKDSFSITLTNTGGCQAVFDEATDSGPHKCPTRRGGRWNSEYDNLSVMNAERVWIDHN
                     SFSDAPRFDTQFPPVFGAPFNETTQKVQHHDGSVDVTLLATKVTISQNYFFRHDKTNL
                     LGGSDFANLVPDYGPGKIDVTFHGNYYQNMIQRAPRVRFGRVHAYNNFFDVERRTTLP
                     GNVAVEYRLGDPWATGTAGKLYTENNLFEIRNNNLTIPRIINYASTVANRDLCVGVGY
                     APQDCGTYYGNTGTWITMIAINSAGTVTGTSTTLFDTFAALKAIQESSAANTPLLKLD
                     PADPAVFWLPSQSYGYVPMPVSTPEERAALRSSIIAGAGAGKL"
     gene            1144..1620
                     /gene="thpR"
                     /locus_tag="VGO02_07055"
     CDS             1144..1620
                     /gene="thpR"
                     /locus_tag="VGO02_07055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEV7477477.1"
                     /translation="MRLFFALWPPRESALALERWAQPLEGRGTPADKIHLTLAFLGEA
                     DAQKALGAARRVQEAGFRLPLEEAGYWGHNKIVWAGPREAPGGLVRLAENLQLELYKD
                     SFILERRPFAAHITLLRKAPAQSLPALPRIEWLAKEFALVRSSGGWYETLERFSLG"
     gene            complement(1612..>1762)
                     /locus_tag="VGO02_07060"
     CDS             complement(1612..>1762)
                     /locus_tag="VGO02_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV7477478.1"
                     /translation="ARGSRSGSGLGLAIVERVARAHGGSLELLPREGGGLVARLTLST
                     VTPLT"
BASE COUNT          320 a          625 c          554 g          263 t
ORIGIN      
        1 catcaacggc aacctgcgcc tgggcttcat caaccagaac gacatcggcg acccggacaa
       61 ctactcggcg aagaacatcg tcgtgcgcaa catcagcttc gccgactcct tcgacatgtt
      121 cccgagctgg gacccgaagg attcgttcag catcacgctc accaataccg gcggctgcca
      181 ggcggtgttc gacgaggcga ccgattccgg cccgcacaag tgcccgacgc gccgcggcgg
      241 ccgctggaat tccgagtacg acaacctctc ggtgatgaac gccgagcgcg tctggatcga
      301 ccacaacagc ttcagcgacg cgccgcgctt cgatacgcag ttcccgccgg tgttcggcgc
      361 gccgttcaac gagacgacgc agaaggttca gcaccacgac ggcagcgtcg acgtgacgct
      421 gctcgccacc aaggtgacga tctcccagaa ctacttcttc cgccacgaca agaccaacct
      481 gctcggcggc tcggacttcg ccaacctggt tcccgactac ggcccgggca agatcgacgt
      541 caccttccac ggtaactact atcagaacat gatccagcgc gcgccgcgcg tgcgcttcgg
      601 ccgggtccac gcctacaaca acttcttcga cgtcgagcgc aggacgacct tgccgggcaa
      661 cgtggcagtg gagtatcgcc ttggcgaccc atgggccacc ggcaccgccg gcaagctcta
      721 caccgagaac aacctgttcg agatccgcaa caacaacctg accatcccgc gcatcatcaa
      781 ctacgcatcg accgtcgcca accgcgactt gtgcgtgggc gtcggctacg cgccccagga
      841 ctgcggtacg tattacggca ataccggcac gtggatcacg atgatcgcca tcaactcggc
      901 gggcacggtc accggtacca gcaccacgct gttcgacacg ttcgccgcgc tcaaggcgat
      961 ccaggaatcg agcgccgcca acacgcccct gctgaagctc gacccggccg atccggcggt
     1021 gttctggctc cccagccaga gctacggcta tgtcccgatg ccggtcagca ccccggagga
     1081 gcgcgccgcg ctgcgctcaa gcatcatcgc cggcgcgggg gccggcaagc tctaagatgt
     1141 gaagtgcgcc tgtttttcgc cctgtggccg ccgcgggaga gtgcgcttgc gctcgagcgc
     1201 tgggcgcagc cgctcgaagg gcgcggcacg ccggccgaca agatccacct gacgctggct
     1261 ttcctgggcg aggccgacgc gcagaaggcg ctcggcgccg cgcgccgggt gcaagaggcg
     1321 gggttccgcc tgccgctcga ggaggccggc tactggggcc acaacaagat cgtgtgggcc
     1381 ggcccgcgcg aggcgcccgg tgggctggtc cggcttgcgg aaaatctcca gctcgagctc
     1441 tacaaggaca gcttcatcct cgagcgaagg ccgtttgccg cgcatatcac cctgctgcgc
     1501 aaggcccccg cgcagtcgct gccggcgctg ccgcggatcg aatggctggc gaaggagttc
     1561 gcgctggtgc gctcctcggg cggctggtac gaaacgctgg agcgtttttc tctaggttaa
     1621 aggcgtcacg gtcgaaagcg taaggcgcgc gaccaggccg ccgccctcgc gcggcaagag
     1681 ctcgagcgag ccgccgtggg cgcgcgcgac gcgctcgacg atcgccaggc ccaggcccga
     1741 gccgctgcgg ctgccccggg cg
//
LOCUS       DASYJA010000321         6401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_774993, whole genome shotgun sequence.
ACCESSION   DASYJA010000321 DASYJA010000000
VERSION     DASYJA010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774993"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..535)
                     /locus_tag="VGO02_07065"
     CDS             complement(116..535)
                     /locus_tag="VGO02_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEV7477479.1"
                     /translation="MAFGGFDKGSPAPMAEINMIPLIDVMLVLLVIFIITAPLLTHAV
                     KVDLPKASSQANQTKPENVQLAIDRDGGVFWNGERVGMAEWRARMQAAAKIEPQPEIH
                     LRADGEVAYRRVAQVMSDAAKTGLTRIGFVTDPNGAN"
     gene            complement(537..1265)
                     /locus_tag="VGO02_07070"
     CDS             complement(537..1265)
                     /locus_tag="VGO02_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEV7477480.1"
                     /translation="MQAQIGFAHFLAQADGVGATLLAFLLLMSVASWYLIVTKAVALW
                     MEQRRSASFLESFWNAQSVGAVQRHLEERHPDEPFSHLAWHAIVASQHHQRHGANRLN
                     EAGSPSEFLTRAIRRVIDEDTARLESGLTLLASVGSTAPFIGLFGTVWGVYHALVAIG
                     LSGQGTLDKVAGPVGEALIMTALGLAVAIPAVLAYNAFVRSNRLVLARLDAFAHDLFA
                     VLTTGSHLGDTGAVVPLKPAAMKV"
     gene            complement(1269..1910)
                     /locus_tag="VGO02_07075"
     CDS             complement(1269..1910)
                     /locus_tag="VGO02_07075"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEV7477481.1"
                     /translation="MAHGAPIAIHPPRTAQRVGAAFAVGMHVLVGAVLLTYEPARSAL
                     LTVAPVMVSLVTSPRPEVKPVQTPPQPKPVVKPRPKPPEPLPLISAPLEAPSSSPILV
                     PPPPPPEPAPVAAPLPAISAPIFAADYLDNPPPSYPTLSRRVGEQGRVVLRVLVSPGG
                     RADDVEIHSSSGYSRLDEAARDTVRRWRFVPAKRGDAAVPAWVLIPVSFRLEG"
     gene            complement(1913..2086)
                     /locus_tag="VGO02_07080"
     CDS             complement(1913..2086)
                     /locus_tag="VGO02_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HEV7477482.1"
                     /translation="MTRSEPQTKSASDAAAEPPARVSSESLLGPRGELLIVHNGREYR
                     LRLTQNGKLILTA"
     gene            complement(2212..3150)
                     /locus_tag="VGO02_07085"
     CDS             complement(2212..3150)
                     /locus_tag="VGO02_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEV7477483.1"
                     /translation="MKPEIIISAKGHAGTMATLQSDFTAHLLSEAPDRAAFLKQHAGT
                     VRGMATFGPMPVDGKLMDALPKLEIISNFGVGVDAINLDDAKQRNIIVTNTPDVLNDC
                     VADTALALIMNTLRRMPQSEAYLRAGKWAARGPYPLATSIGGKTLGILGLGRIGEAIA
                     KRAMACGMKIRYHNRNKKDVPYPYDPDPVSLAKNSNVLMIVTPGGAETRNLVGAKVLD
                     ALGPKGYVCNIARGSVIDEPVLLRYLQENKIAGAGLDVFADEPRVPPEFFTLENAVLF
                     PHVGSATVETRKAMGDLQIENLRRHFSGQPVLTRVA"
     gene            complement(3160..3924)
                     /locus_tag="VGO02_07090"
     CDS             complement(3160..3924)
                     /locus_tag="VGO02_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HEV7477484.1"
                     /translation="MDVPKNQFKSALKSNQSQIGLWSSLSSAYTVEVIAGAGFDWILL
                     DSEHSPADLENLLTQLQAAAPYPGHAVVRVPWNDMVTIKRTLDVGAQSLLIPYVSTAA
                     EARDAVSYTRYPPAGVRGVAGTTRATRFGRVKDYAKRAHEEICVLVQVETQGALDNIE
                     AICAVEGVDGVFIGPADLHASLGYTGEIANPKVKPLIDDAIRRIRKCGKAPGILTPNE
                     ADARHWLECGALFVAVGSDVGILARGAEALAAKFKS"
     gene            complement(3934..4803)
                     /locus_tag="VGO02_07095"
     CDS             complement(3934..4803)
                     /locus_tag="VGO02_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV7477485.1"
                     /translation="MSDKSESTGMEYLQSLPRRWVTLYVPVGVFLFVLLFPFYWMVMT
                     SFKSNEELVSKAANPFWIVKPTLDHFEKLLFRTEYPQWLWNTMLVSVAATFISLFCAV
                     LAAYAIERLRFRGSRQVGLSIFFAYLVPPSILFIPLALIVFKLGLFDSTWALILTYPT
                     FLIPFCTWLLMGYFRTIPFELEECALIDGATRWQILLKIMLPLAVPGLISAGIFAFTL
                     SWNEFIYALTFISSSENKTVPVGVITELVEGDVYHWGALMAGALLGSLPVAILYSFFV
                     EYYVSGMTGAVKE"
     gene            complement(4800..5705)
                     /locus_tag="VGO02_07100"
     CDS             complement(4800..5705)
                     /locus_tag="VGO02_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV7477486.1"
                     /translation="MTLSRFFDNRNTLGLLFMLPAGVLLLLFLTYPLGLGTWLGFTDA
                     KIGRSGEWIGLENFQFLVGDAVALLSLFNTLFYTVVASVLKFVLGLWLALLLNRHLPF
                     KSFIRAIVLLPFIVPTALSAIAFWWIYDSQFSVVSWTLTKMGLIHRYIDFLGDPWMAR
                     LSVIAANVWRGVPFVAISLLAGLQTIPPSLYEASSIDGATPWQQFWNVTLPLLTPIIA
                     VVMTFSVLFTFTDFQLIYVLTRGGPLNATHLMATLSFQRGIPGGALGEGAALAMLMVP
                     FLLAAILFSYFGLQRRAWQQGGADK"
     gene            complement(5705..>6401)
                     /locus_tag="VGO02_07105"
     CDS             complement(5705..>6401)
                     /locus_tag="VGO02_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV7477487.1"
                     /translation="TWCHWVVWAFGGKMVDKSGKVVIDSPETIKALEYSKELYGTFAP
                     GTLSWLDPSNNKAFLDGQISVTNNGISIYYAAKTSKDPKINALAADINHSVYPAGPGG
                     SNAAFHLFLNQMVFKYTKYPKAAKEFLRFMMEEEQFGPWMQASIGYVAHPLAAYAKNP
                     IWTVDPKHLPYRDAVKELRPAGYDGKLGYASAGALADFIMVDMVAEAASGSKPPKEAA
                     ERAQKRAERYYKV"
BASE COUNT         1110 a         2015 c         2227 g         1049 t
ORIGIN      
        1 acggcgggaa gaagcgcctc gcgcccggtt ttcctgaggt aagctgcggc gatggcgacg
       61 atcaggaaca tttcgattcc ctcgcgcagc gtgactacca gcatctggcc catggctagt
      121 tggctccgtt cgggtcggtg acgaagccga tgcgcgtcag gccggtcttg gcggcatcgg
      181 acatcacttg ggcgacgcgc cggtaggcga cctcgccgtc ggcacgcagg tggatctccg
      241 gctgcggctc gatcttcgct gctgcctgca tgcgagcgcg ccattccgcc atgccgacgc
      301 gctcgccgtt ccagaacacc ccgccgtccc ggtcgatcgc gagctgcacg ttttccggct
      361 tggtctggtt ggcctggctc gaggccttcg gcaggtccac cttcacggcg tgggtgagga
      421 gcggcgccgt gatgatgaag atcaccagca gcaccagcat gacgtcgatc agcgggatca
      481 tgttgatctc cgccatcggg gcgggactgc ctttgtcgaa gccgccgaaa gccatgtcag
      541 actttcattg cggcgggctt gagcggcacc acggcgccgg tatcgcctag atgcgagccg
      601 gtggtgagca ccgcgaagag gtcgtgcgcg aaggcgtcca ggcgcgcgag caccagccgg
      661 tttgagcgca cgaaggcgtt gtaggcgagc accgccggga tcgccacggc gagaccgagc
      721 gcggtcatga tcagtgcctc gcccaccggg ccagcgacct tgtcgagggt gccctggccg
      781 gacaggccga tcgccaccag cgcgtgatag acgccccaga ccgtgccgaa caggccgatg
      841 aagggcgcgg tcgagccgac ggaggcgagc agggtcaggc ccgattcgag gcgcgcggtg
      901 tcctcgtcga tgacgcggcg tatcgctcgc gtgaggaact cggacggcga accggcctca
      961 ttgaggcggt tggcgccgtg cctctggtgg tgctgcgagg cgacgatggc gtgccaggcg
     1021 aggtgcgaga acggctcgtc ggggtgacgc tcttccaggt ggcgctgcac cgcgccgacc
     1081 gactgcgcgt tccagaagct ctccaggaag ctcgcgctgc ggcgctgctc catccagagc
     1141 gcgaccgcct tggtgacgat caggtaccag ctcgcgaccg acatgaggag caggaaggcg
     1201 agcagggtcg cgcccacgcc gtcggcctgc gcaaggaaat gggcgaatcc gatctgcgcc
     1261 tgcatggcct agccttccag tctgaaagaa accgggatca ggacccacgc cggcaccgcc
     1321 gcgtcgccac gcttcgccgg gacgaagcgc cagcggcgca ccgtgtcgcg cgcagcctcg
     1381 tcgagccggg agtagccgct cgagctgtgg atctcgacgt cgtccgcgcg gccgccgggg
     1441 ctgaccagca cgcgcagaac gacgcgtccc tgctcgccga cgcgtcgcga gagggtcggg
     1501 taggacggcg gcggattgtc gaggtagtcc gcggcgaaga tcggcgcgct gatcgccggc
     1561 agcggggccg cgacgggcgc gggctccggg ggtggcggcg gcggcaccag aatcggcgag
     1621 gacgaaggcg cctccagcgg agcgctgatc agcggcagtg gctcgggtgg cttcggccgc
     1681 ggcttcacca ccggcttggg ttgcggcggt gtctgcaccg gcttgacctc cggccggggt
     1741 gacgtgacga gactgaccat gactggcgcc acggtgagca gcgcagagcg cgccggctca
     1801 taggtgagga gcacagcgcc gaccagcacg tgcatgccaa ccgcgaacgc cgcgccgacg
     1861 cgttgcgcgg tacgcggcgg atggatagcg attggcgcgc cgtgtgccat ctctacgcgg
     1921 tcaggatcag cttgccgttt tgcgtgagcc gcaggcggta ctcgcggccg ttgtgcacga
     1981 tcagcagctc gccacgcggt ccgagcagcg actcgcttga cacccgcgca ggtggctccg
     2041 ctgccgcgtc gctggcgctc ttggtctggg gctccgatct ggtcactcga caacgataat
     2101 agttctcatt atcgcagtca atcgttcccg gcgcgtccag cttaaagtta gccgatagtg
     2161 atcgctaact ttaagcgaca actctttgct ttaattgata atgcttcgcg tttaggcgac
     2221 gcgggtcagc accggctggc cggagaagtg gcggcgcagg ttctcgatct gcaggtcgcc
     2281 catcgccttg cgcgtctcga cggtagcgct gccgacgtgc gggaagagca ccgcgttctc
     2341 gagcgtgaag aactcgggcg gcacgcgcgg ctcgtcggcg aatacgtcca ggccggcacc
     2401 ggcgatcttg ttctcctgca ggtagcgcag cagcaccggc tcgtcgatca ccgagccgcg
     2461 cgcgatgttg cagacatagc ccttcggacc gagggcatcg agcaccttgg cgccgaccag
     2521 gttgcgcgtt tccgcgccgc cgggggtcac gatcatcagc acgttgctgt tcttcgccag
     2581 cgacaccgga tccgggtcgt acgggtaagg cacgtccttc ttgttccggt tgtgatagcg
     2641 gatcttcatg ccgcaggcca tggcgcgctt ggcgatggcc tcgccgatgc ggcccaggcc
     2701 gaggatgccg agcgtcttgc cgccgatgga ggtggcgagc gggtaggggc cgcgcgcggc
     2761 ccacttgccg gcgcgcaggt aggcctccga ttgcggcatc ctgcgcagcg tgttcatgat
     2821 gagcgcgagc gccgtgtcgg cgacgcagtc gttcagcacg tcgggcgtgt tggtgacgat
     2881 gatgttgcgc tgcttcgcgt cgtcgaggtt gatggcgtcc acgccgacgc cgaagttcga
     2941 gatgatctcg agcttgggca gcgcgtccat cagcttgccg tcgaccggca tcggcccgaa
     3001 ggtggccatg ccgcgcacgg tcccggcgtg ctgcttcagg aacgcggcgc gatcgggcgc
     3061 ctccgacagg aggtgcgcgg tgaagtccga ctgcagggtg gccatcgtgc cggcgtggcc
     3121 cttggcggag atgatgatct cgggtttcat ggctctaggt tatgacttga attttgccgc
     3181 gagggcctcg gcgccgcgcg cgaggatgcc gacgtcggag ccgacggcga cgaacagcgc
     3241 gccgcactcc agccagtggc gcgcgtcggc ctcgttgggc gtcaggatgc cgggcgcctt
     3301 gccgcacttg cggatgcggc ggatggcatc gtcgatcagc ggcttcacct tgggattggc
     3361 gatctcgccg gtgtagccga gcgaggcgtg caggtcggcg gggccgatga acacgccgtc
     3421 caccccctcg acggcgcaga tggcctcgat gttgtcgagc gcgccctgcg tctcgacctg
     3481 caccagcacg cagatctcct cgtgcgcgcg cttggcgtag tccttgacgc ggccgaagcg
     3541 cgtggcgcgg gtggtgcccg cgaccccgcg cactccggcc ggcggatagc gcgtatacga
     3601 cacggcgtcg cgcgcttccg ccgccgtgga aacgtaaggg atgagcagcg actgcgcgcc
     3661 gacatccagc gtgcgcttga tcgtcaccat gtcgttccac ggcacgcgta ccaccgcatg
     3721 gccgggatag ggcgccgccg cctgcagctg cgtcagcagg ttctcgaggt cggccggaga
     3781 gtgctcgctg tcgagcagga tccagtcgaa cccggcgccg gcgatgactt ccacggtgta
     3841 ggccgaggag aggctcgacc agaggccgat ctgcgattgg ttggacttga gggcgctttt
     3901 gaactggttt tttggaacgt ccatgcggct attttattct tttaccgcgc cggtcatccc
     3961 cgagacgtaa tactcgacga agaacgagta caggatcgca accggcagcg agccgagcag
     4021 cgcgcccgcc atcagcgcgc cccaatggta gacgtcgccc tccaccagct cggtgatcac
     4081 gccgaccggc acggtcttgt tctccgagga ggaaatgaag gtcagggcgt agatgaactc
     4141 gttccacgac agcgtgaagg cgaagatgcc ggccgagatg aggccgggca cggcgagcgg
     4201 cagcatgatc ttcaaaagga tctgccagcg cgtcgcgccg tcgatgagcg cgcattcctc
     4261 cagctcgaac gggatggtgc ggaagtagcc catcagcagc caggtgcaga acgggatcag
     4321 gaacgtcggg taggtgagga tcagcgccca ggtggaatcg aacaggccga gcttgaacac
     4381 gatcagcgcg agcgggatga acaggatcga gggcggcacc aagtaggcga agaagatcga
     4441 caggccgacc tggcgcgagc cgcggaaacg cagccgctcg atggcgtagg cggcgagcac
     4501 cgcgcagaac agcgagatga aagtcgccgc caccgagacc agcatggtgt tccacagcca
     4561 ctgcggatac tcggtgcgga aaagcagctt ctcgaaatgg tcgagcgtcg gttttacgat
     4621 ccagaacggg ttggccgcct tggacaccag ctcctcgttc gacttgaacg aggtcatcac
     4681 catccagtag aaagggaaca gcagcacgaa caggaagaca ccgaccggga cgtagagcgt
     4741 gacccagcgg cggggcagcg attgaaggta ctccatcccc gtgctttcgc tcttgtcgct
     4801 catttgtcgg cgcctccctg ctgccaggcg cggcgctgca gcccgaagta ggagaacagg
     4861 atcgccgcca gcaggaaagg caccatcagc atggcgagcg cggcgccttc gcccagcgcg
     4921 cctcccggga tgccgcgctg gaacgacagc gtggccatga ggtgggtggc gttgagcggc
     4981 ccgccgcgcg tcagcacgta gatgagctgg aaatccgtga acgtgaacag cacgctgaag
     5041 gtcatcacca cggcgatgat cggcgtgagc agcggcagcg tgacgttcca gaactgctgc
     5101 cacggtgtcg cgccgtcgat cgaggacgct tcgtacagcg aaggcgggat ggtctgcagc
     5161 cccgccagca gggaaatcgc cacgaacggc acgccgcgcc agacgttcgc cgcgatgacc
     5221 gacagccgcg ccatccacgg gtcgccgagg aagtcgatgt agcggtggat cagccccatc
     5281 ttggtcagcg tccagctcac caccgagaac tgcgagtcgt agatccacca gaaggcgatc
     5341 gccgaaaggg cggtcggcac gatgaacggc agcagcacga tcgcacggat gaatgacttg
     5401 aagggaaggt gccggttgag gagcagcgcg agccagaggc cgagcacgaa cttgagcacg
     5461 ctcgccacga cggtgtagaa gagcgtgttg aacagcgaaa gcagggcgac ggcgtcgccg
     5521 accaggaact ggaagttctc caggccgatc cattcgccgc tgcggccgat cttcgcgtcg
     5581 gtgaagccca gccaggtgcc caggcccagc ggataggtca ggaaaagcag gagcagaaca
     5641 cccgcgggca gcatgaacag cagcccgagg gtgttccggt tgtcgaagaa cctgctgagc
     5701 gtcatcagac cttgtagtag cgctcggccc gcttctgcgc ccgctcggcg gcttccttcg
     5761 gcggcttcga gcccgatgcc gcctccgcca ccatgtcgac catgatgaag tcggcgagcg
     5821 cgcccgccga ggcgtagccg agcttgccgt cgtagccggc ggggcgcagc tccttcaccg
     5881 cgtcgcgata gggcagatgc ttcggatcga ctgtccagat cgggttcttc gcatacgccg
     5941 ccagcgggtg cgcgacgtag ccgatgctcg cctgcatcca tggcccgaac tgctcctcct
     6001 ccatcatgaa gcgcaggaac tccttcgccg ccttcgggta cttggtgtac ttgaagacca
     6061 tctggttgag gaacaggtgg aacgccgcgt tcgagccgcc gggcccggcc ggatagaccg
     6121 agtggttgat gtccgcggcc agcgcattga tcttcggatc cttcgaggtc ttggccgcgt
     6181 agtagatcga gatgccgttg ttggtcaccg agatctggcc gtcgaggaac gccttgttgt
     6241 tgctcgggtc gagccaagac agcgtgccgg gggcgaaggt gccgtagagc tccttcgagt
     6301 actcgagcgc cttgatggtc tcggggctgt cgatcaccac cttgcccgac ttgtccacca
     6361 tcttgccgcc gaaggcccac accacccagt ggcaccaggt a
//
LOCUS       DASYJA010000322         3221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2044983, whole genome shotgun sequence.
ACCESSION   DASYJA010000322 DASYJA010000000
VERSION     DASYJA010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2044983"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1108)
                     /locus_tag="VGO02_07110"
     CDS             complement(167..1108)
                     /locus_tag="VGO02_07110"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004554989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEV7477488.1"
                     /translation="MLGAISQVLEIMVALVLAPLLVGWVNQWRAWLQNRSAPGVLQPY
                     RVLHKLFMKDVVMAENASPLFRAAPYVVFGCMTLACAIIPTLSTDLPLSPAADAIALV
                     GLFALARVFISLAAMDVGTAFGSMGARREMLIGFLAEPALLMVLFTAALISHSTSLTT
                     IVDTLAHRELAIYPSLAFAGVAFTIVSLAENARVPVDNPATHLELTMVHEAMILEYSG
                     RHLALMEWAAALKLFAYSCAGLALFFPWGVAQADAPLALLLAAPILVVKLALGGVALA
                     VLETLSAKMRIFRVPEFLATAFLLAVIAMLVNILLGA"
     gene            complement(1108..3126)
                     /gene="hyfB"
                     /locus_tag="VGO02_07115"
     CDS             complement(1108..3126)
                     /gene="hyfB"
                     /locus_tag="VGO02_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HEV7477489.1"
                     /translation="MADARLRERRLSLVLGVVGAWLLVGAAGLFALQRLRFVARVLFP
                     LGGLLGLVLAALAAAALFGAPQTAVLPIGLPDLPFHFRLDGLSAFFLMVIAAAAAGVS
                     AFASGYFGKREGAPPGLLCLQYHVFLAAMALVVLADDAYSFMVMWETMALSSFFLVTT
                     NHRIAEIRRAGYLYLLIAHIGAIAILLCFGVLQANTGDYGFDNMRAQALSPFWASIAF
                     LLALFGFGAKAGLLPLHVWLPEAHPAAPSPVSALMSGVMLKTAVYGFLRVTYDLLDAQ
                     IWWWGFVALAVGLASALFGVVFAAVQVDMKRLLAYSSIENIGLMFAAIGLSILFSAYG
                     MKPMAALALTAALYHVASHAFFKSLLFVATGAVLHATSERSLGRLGGLIRYMPWVAWL
                     ALVGALASAGLPPFGGFVAEWLLLQSFLFTPGLPNSFLNMLVPVATALIALVAALAGY
                     TMVKFFGVVFLGRPREEKLAAAQDAGGWERAGMVFLAAGCIALGLAPVQFIQAIDPVT
                     RQLVGGGLGETVAASGWLLVPVSIASASYGPAVFLAGVAASFLLAFVLVRAFYHGRLR
                     RSPPWDCGFPWQTPRMQDTAEGFGQPIRQIFEPLFRMQRELPTPFDAAPRYYVKVEDP
                     FWYWVYLPVALAVERTARLVGLLQQGRIAVYLLYSFVTVIAVLIWVTR"
BASE COUNT          634 a         1072 c         1162 g          353 t
ORIGIN      
        1 ggctggtgcg tcgcgtagcc gacgatggcg gtggaggcga ccagcgtcgc gccctgcagg
       61 gcgaacaggt ggatcagcga aagcacccgc cgctgcgaga tcatggcgaa ggcgagcagc
      121 agcatcagcg cggcgaacag gttgatgagc tgggaggcga gcgggctcac gcgccgagaa
      181 ggatgttcac cagcatggcg atcaccgcca gcaggaacgc ggtggcgagg aattcgggca
      241 cgcggaagat gcgcatcttc gcgctcagcg tctccaggac ggcgagggcc acgccgccaa
      301 gcgccagctt caccaccagg atcggcgcgg caagcagcag cgcgagcggc gcgtcggcct
      361 gcgccacgcc ccatgggaag aacagcgcca gcccggcgca ggagtaggcg aagagcttga
      421 gcgccgccgc ccattccatc agcgccaggt ggcgtcccga atactcgagg atcatcgcct
      481 catgcaccat ggtcagctcc aggtgcgtcg ccgggttgtc gaccggcacg cgcgcattct
      541 cggcgaggga gacgatggtg aaggccacgc cggcgaaggc caggctcggg tagatggcga
      601 gctcgcggtg cgcgagcgtg tcgacgatgg tggtgagcga cgtcgaatgc gagatcagcg
      661 ccgccgtgaa aagcaccatc agcagcgccg gctcggcgag aaagccgatc agcatctcgc
      721 ggcgagcgcc catcgagccg aacgcggtgc ccacatccat ggcggcgagc gagatgaaga
      781 cgcgcgccag cgcgaacagg ccgaccagcg cgatggcgtc ggcggcgggc gagagcggca
      841 ggtcggtcga cagcgtcggg atgatggcgc aggcgagcgt catgcagccg aacaccacat
      901 acggcgccgc acggaacagc ggcgaggcgt tttccgccat caccacgtcc ttcatgaaca
      961 gcttgtgcag cacgcggtag ggctgcagca cccccggggc ggacctgttc tgcagccagg
     1021 cgcgccactg gttcacccag ccgacgagca agggcgcgag cacgagcgcc accatgatct
     1081 ccaggacctg ggaaatcgcg cccagcatca gcgcgtcacc cagatcagga cggcgatgac
     1141 ggtgacgaag ctgtagagca gatacacggc aatgcggccc tgctgcagca ggccgaccag
     1201 gcgcgcggtg cgctcgacgg cgagcgcgac cggcaggtac acccaatacc agaagggatc
     1261 ctcgaccttg acgtagtagc gcggcgcggc atcgaacggc gtaggcagct cgcgctgcat
     1321 gcgaaacagc ggctcgaaga tctggcggat cggctgcccg aagccctcgg ccgtgtcctg
     1381 catgcgcggc gtctgccacg gaaagccgca gtcccacggc ggcgagcgcc gcaggcggcc
     1441 gtggtagaac gcgcgcacca gcacgaaggc gagcagaaag ctcgcggcca cgccggcaag
     1501 gaataccgcc ggcccgtagc tcgccgaagc gatgctcacc ggcaccagca gccagccgct
     1561 ggcggccacc gtttcgccga gcccgccgcc gaccagctgg cgggtgaccg ggtcgatcgc
     1621 ctggatgaac tgcaccggag cgaggccgag cgcgatgcac ccggcggcca ggaacaccat
     1681 gccggcgcgc tcccagccgc cggcatcttg cgcggcggcg agcttttcct cgcgcgggcg
     1741 gccgaggaaa accacgccga agaacttcac catggtgtag ccggcgagcg cggcgaccag
     1801 cgcgatcagc gccgtggcca ccggcaccag catgttgagg aaggagttgg gcaggcccgg
     1861 ggtgaagaga aagctctgca gcagcagcca ctccgcgacg aagccgccga agggcggcag
     1921 gccggcgctt gccagcgcgc cgaccagcgc cagccaggcg acccagggca tgtagcggat
     1981 caggccgccg aggcggccca ggctgcgctc gctggtagcg tgcagcaccg cgcccgtggc
     2041 gacgaacagc aggctcttga agaaggcgtg gctcgcgacg tggtagagcg ccgcggtgag
     2101 ggccagcgcc gccatcggct tcatgccgta cgcggaaaac aggatcgaca gtccgatggc
     2161 cgcgaacatc agcccgatgt tctcgatcga ggagtaggcg agcagccgct tcatatccac
     2221 ctggacggcg gcgaacacca cgccgaacag cgcgctggcg aggccgacgg cgagggcgac
     2281 aaatccccac caccagatct gcgcatcgag caggtcgtag gtgacgcgca ggaagccgta
     2341 gaccgcggtc ttgagcatca cgccgctcat cagcgcggac acgggcgacg gtgcggccgg
     2401 gtgcgcctcg ggcagccaga cgtgcagcgg cagcagcccg gccttggcgc cgaagccgaa
     2461 cagcgcgagc aggaaggcga tcgacgccca gaaaggcgac agcgcctgcg cgcgcatgtt
     2521 gtcgaagcca tagtcgccgg tgtttgcctg cagcaccccg aagcacagca ggatggcgat
     2581 ggcgccgatg tgggcgatga gcaggtacag gtagccggcg cgccggatct cggcaatgcg
     2641 gtggttggtg gtgacgagga agaacgacga cagcgccatc gtctcccaca tcaccatgaa
     2701 cgaatacgcg tcgtcggcga gcacgaccag ggccatggcg gcgaggaaga cgtggtactg
     2761 caggcacagc agtcccggcg gcgcgccttc ccgcttgccg aagtagcccg aggcgaacgc
     2821 cgagacgccg gcggccgcgg cggcgatcac catcaggaag aacgccgaca gtccgtccag
     2881 gcggaaatgg aacggcaggt ccggcagccc gatcggcagc acggcggttt gcggcgcgcc
     2941 gaacagcgcg gccgcggcca gcgcggcgag cactagcccg agcaggccgc cgagggggaa
     3001 caggacccgg gccacgaagc gcaggcgctg cagcgcgaac agtccagcgg cgccaaccag
     3061 cagccaggcg ccgacgacgc ccagtaccag gctcagccgt cgctcccgga ggcgcgcgtc
     3121 agccagccgg gaagctcgcg gccgccggcg ttctcgatga tccattggcg gatcagcttg
     3181 cgtacgacct gcgaaggcgt cacgtcctgc atggcgcaca g
//
LOCUS       DASYJA010000323         3639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2407820, whole genome shotgun sequence.
ACCESSION   DASYJA010000323 DASYJA010000000
VERSION     DASYJA010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2407820"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="VGO02_07120"
     CDS             complement(<1..944)
                     /locus_tag="VGO02_07120"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEV7477490.1"
                     /translation="MGGYYTCVPHPYATLDPVAVLDALDTVGLRGDGRLLTLNSYENR
                     VYQVWLEDNSSVVVKFYRPGRWSAAQIAEEHAFAKELAEREIPVVAPLQQGTFKEFIF
                     AAYPRRGGRTPELDKPDVLEWIGRFLGRIHAVGATRPFVERPSLNQETFGAEPRAWLL
                     EHNCVPAELLDAWKAITEQALSAVHRCFSLAGNPKQIRLHGDCHPGNILWTDGIEGAG
                     PHFVDLDDARMGPAVQDLWMLLSGDRATQTRALSDVLAGYEDFMELDRRELNLVEALR
                     TLRLIHYSAWIARRWDDPAFPAAFPWFNTTRYWQDRIL"
     gene            943..1746
                     /locus_tag="VGO02_07125"
     CDS             943..1746
                     /locus_tag="VGO02_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7477491.1"
                     /translation="MTQNAVEVRDLNFSYGELQIFRRLSLDIPRGKVVAILGGSGSGK
                     STLLKLIGGQLRPASGSVMLEGRNVHQLSSDELYRLRLEIGMMFQVSGLFSDLSVYDN
                     IAFPIRENFDLPEEIVRRMVLMKLHAVGLRGARDMMPGDLSGGMMRRVALARAIATDP
                     KLLMYDEPFAGLDPISLTQIGELISTLNQALGLTSIVVTYDVHEALKVAGYVYVINDG
                     AVLGKGTPDEIASSEDPYLKQFVNALPDGPVRFHFPAAPVAEDLNLPPG"
     gene            complement(1743..2279)
                     /gene="msrB"
                     /locus_tag="VGO02_07130"
     CDS             complement(1743..2279)
                     /gene="msrB"
                     /locus_tag="VGO02_07130"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV7477492.1"
                     /translation="MRRRTFLLGLALAPLAGRAAQPVDMKDIKRMQSDWKTLLAPGTT
                     VPSPTEPLKLSKEEWRKRLPPQAYGVLREENTEHPGTSQLNHEKRPGVFVCAGCGLPL
                     FTSEMKFESGTGWPSFFTAIPGAFETKKDFYLIYPRTEYHCARCGGHHGHLFDDGPPP
                     TSQRWCNNGVALRFIPRS"
     gene            complement(2346..2654)
                     /locus_tag="VGO02_07135"
     CDS             complement(2346..2654)
                     /locus_tag="VGO02_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477493.1"
                     /translation="MLKKTILATLIGSTLLASSASFADPYYRHGHGYGHREVVVQRYY
                     QPARPVVVQRYYQARPVIVERRVVVQRPAPVYAYGNDGLAILAGAVLGGAIAYQIANH
                     "
     gene            2771..>3639
                     /locus_tag="VGO02_07140"
     CDS             2771..>3639
                     /locus_tag="VGO02_07140"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7477494.1"
                     /translation="MSRFIGGFLLFFFTVHGWGQDAYPSKPITMIVPFPPGGVAEIVG
                     RPLAVELEKSLRQPVVLINRPGAGGAVGMASVARAPGDGYTLLMGLSSISIFPVSDRI
                     NGKTPAYELKDFAPIALITADPTVLVVRTDGPYKTLKDFVDAAKANPGKINYSSSGVY
                     GTLHVSMEIFANAAGIKLFHVPYQGGGPAVTALLGGQVEALASGPAPAIGQIRGGKMR
                     ALASWSSERLALLPEIPTFKELGYDAEFYIWTGVFAPATTPPNILSRIRNAVKEAANS
                     AEFKAAMEKVQTPV"
BASE COUNT          598 a         1191 c         1240 g          610 t
ORIGIN      
        1 tcgaggatcc ggtcctgcca gtagcgcgtg gtgttgaacc acgggaaggc ggcggggaag
       61 gcggggtcgt cccagcgccg cgcgatccac gccgagtaat ggatcaggcg cagcgtgcgc
      121 agcgcctcga ccaggttcaa ctcgcgccgg tcgagctcca tgaagtcctc gtagcccgcg
      181 agcacgtcgg acaaggcgcg cgtctgggtg gcgcggtcgc ccgagagcag catccacagg
      241 tcctgcaccg ccggccccat gcgcgcgtcg tccaggtcga cgaaatgcgg acccgcccct
      301 tcgatgccgt cggtccacag gatgttgccg ggatggcagt cgccgtgcag gcggatctgc
      361 tttggatttc ccgccaggga aaaacatctg tgcacggcgg acagcgcctg ctcggtaatc
      421 gccttccagg catccagcag ttccgccggg acgcagttgt gctcgagcag ccaggcgcgc
      481 ggctcggcgc cgaaggtctc ctggttgagg gacggtcgct ccacgaaagg ccgcgtcgcg
      541 cctacggcat ggatgcggcc gaggaagcgg ccgatccatt cgagcacgtc gggcttgtcg
      601 agctcgggcg tgcgcccgcc gcggcgcgga taggccgcga agatgaactc cttgaaggtg
      661 ccttgctgca agggagcgac gacggggatt tcgcgctcgg cgagctcctt cgcgaatgca
      721 tgctcctcgg caatctgcgc cgcgctccag cgcccgggac ggtagaactt cacgactacg
      781 gaggagttgt cctccagcca tacctggtag acgcggttct catagctgtt gagggtaagc
      841 agccggccgt cgccgcgcag gccgaccgtg tcgagcgcgt cgagtacggc caccgggtcg
      901 agcgtcgcgt aggggtgggg cacgcaggta tagtagcccc ccatgacgca gaacgcagtc
      961 gaggtgcggg acctcaattt ctcctacggc gagctgcaga tcttccgccg gctgtcgctc
     1021 gacattccgc gcggcaaggt agtggcgatc ctcggcggca gcggctcggg caagtcgacg
     1081 ctgctcaagc tgatcggcgg ccagctgcgc ccggcgagcg gcagcgtgat gctcgagggc
     1141 cgcaacgtgc accagctcag ctccgacgag ctgtaccgcc tgcgcctcga gatcggcatg
     1201 atgttccagg tctcggggct gttcagcgac ctctcggtgt acgacaacat cgccttcccg
     1261 atccgcgaga acttcgacct gcccgaggag atcgtgcgcc gcatggtgct gatgaagctg
     1321 cacgcggtcg ggctgcgcgg cgcgcgcgac atgatgccgg gcgacctgtc gggcggcatg
     1381 atgcgccgcg tcgcgctggc gcgcgccatc gccaccgacc cgaagctgct gatgtacgac
     1441 gagcccttcg ccggcctgga cccgatctcg ctcacccaga tcggcgagct gatcagcacc
     1501 ctgaaccagg ccctcggcct gacctcgatc gtcgtcacct acgacgtgca cgaggcgctg
     1561 aaggtggcgg ggtacgtcta tgtgatcaac gacggcgcgg tgctcggcaa gggcacgccc
     1621 gacgagatcg cgtcgtcaga ggacccttac ctcaagcagt tcgtcaacgc gctgcccgac
     1681 ggcccggtgc gcttccactt cccggccgcg ccggtggccg aggacctcaa cctgccgccg
     1741 ggttaactgc gcgggataaa gcgcagcgcg acgccgttgt tgcaccagcg ctggctggtc
     1801 ggcggcggcc cgtcgtcgaa gaggtgcccg tggtgcccgc cgcagcgcgc gcagtggtac
     1861 tcggtgcgcg ggtagatgag gtagaagtcc ttcttggtct cgaaggcgcc cgggatggcg
     1921 gtgaagaagc tcggccagcc ggtgcccgac tcgaacttca tctcggaggt gaagagcggc
     1981 agcccgcagc cggcgcagac gaatacgccc gggcgcttct cgtggttgag ctggctggtg
     2041 ccggggtgct cggtgttctc ctcgcgcagc acgccatagg cctgcggcgg caggcgcttg
     2101 cgccactcct ccttcgacag cttgagcggc tcggtgggcg agggcacggt cgtgcccggg
     2161 gcaagcaagg tcttccagtc gctttgcatc cgcttgatgt ccttcatgtc gacgggctgc
     2221 gccgcgcgcc cggcgagcgg ggcgagagcc agcccaagaa gaaaagtcct gcgtctcatt
     2281 actttgatct ccgaaaatgc atccgcccgg cttggtcggc cgggcggtcg ggaccttaca
     2341 ggatatcagt ggttggcgat ctggtacgcg atcgcgccgc cgagcacggc gcccgccaag
     2401 atggcgaggc catcgttgcc gtacgcatat accggcgccg ggcgctgcac cacgacacgt
     2461 cgctccacga tgaccgggcg cgcctggtag taacgctgca ccaccactgg ccgcgccggc
     2521 tggtaatagc gctgcaccac gacctcgcgg tggccgtagc cgtgcccgtg gcgatagtag
     2581 ggatcggcaa acgaggcgct cgaggccaac agggtgctac cgatcagcgt ggcgaggatt
     2641 gtcttcttaa gcatggcagg ctcctttcaa gttgagtgcc tggctatttg acgcgccgcc
     2701 gcgcaaaacg cgaacccaga aagtgcaaca tgtgtaaccg catgtatatt catgccgcag
     2761 gaggcggcat atgtctcgat ttatcggtgg ttttcttctt tttttcttca cagttcacgg
     2821 ctggggccag gacgcctatc cgtccaagcc catcaccatg atcgtgccct tcccgccggg
     2881 cggagtggcc gagatcgtcg ggcggccgct cgccgtggag ctggagaaat cgctgcgcca
     2941 gccggtggtg ctcatcaacc ggcccggcgc gggcggcgcg gtgggcatgg cttcggtggc
     3001 cagggcaccg ggggacggct acacgctgct gatgggcctg tcctcgattt ccattttccc
     3061 ggtgtccgac cgcatcaacg gcaagacgcc cgcctatgag ctgaaggatt tcgcgccgat
     3121 cgcgctgatc accgccgacc ccaccgtgct ggtggtgcgc accgacggtc cgtacaagac
     3181 gctgaaggac ttcgttgacg ccgcgaaggc caaccccggc aagatcaact attcttcctc
     3241 gggcgtgtac ggcacgctgc acgtctcgat ggagatcttc gccaacgccg ccgggatcaa
     3301 gctgttccac gtgccctacc agggcggcgg cccggcggtg accgcgctgc tcggcggcca
     3361 ggtcgaggcg ctcgcctcgg ggccggcgcc ggcgatcggg cagatccggg gcggcaagat
     3421 gcgcgcgctt gccagctgga gcagcgagcg cctcgccctg ctgcccgaga tcccgacctt
     3481 caaggagctc ggctacgacg ccgagttcta catctggacc ggcgtcttcg cgccggcgac
     3541 cacgccgccc aacattcttt caagaataag aaatgcagtg aaagaagcgg cgaactccgc
     3601 ggagttcaag gccgcgatgg agaaggtgca gacgccggt
//
LOCUS       DASYJA010000324         2755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_593237, whole genome shotgun sequence.
ACCESSION   DASYJA010000324 DASYJA010000000
VERSION     DASYJA010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593237"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1818)
                     /locus_tag="VGO02_07145"
     CDS             complement(175..1818)
                     /locus_tag="VGO02_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477495.1"
                     /translation="MYYNPGDGEDIVVPRGYKVEVFAKDLNFPTDVAFLGDKHNFRVL
                     VLESGTGLPSQCNDNNRVPGIGKFDPRNPFTPGLFIYDRNGRKLAGPLGKPTPSGGGF
                     QPDGPAIGLSFEHDTEGGTLFATDSNQGVRGAPGSANNTSRIVRVNISSGSVTPLITG
                     LPTGDHPTEQIIVKDGWLYWSQGSATNSGVTGHDNGGGGNQHDIACETVKLSQNTWDS
                     GDGHHTSGYSNHGVARPGAVVPAFEGATRRGMCTGAILRAKISNPANSIEPVAWGFRN
                     PYGLRFSPSDHPLKGELFVSENGEDERGARPVNNAPDRLALARQNPDGSPSYHGWPDR
                     FGFLESTQAVFNPIGGAGDDLWPDVARIRAEDVPVKPVFAFPPRPPVAPLALEPADVA
                     AVGLDFVPKSFVHGVVKKHAVLVSREGDFGFSPENGNPSAGHDVELVNFTSDDDPAEL
                     LLSRFAFNCARSEQRHDADGTPRCNGDSDQAFTQMLRGINRPTTIRFGPDGAAYLVDY
                     GAVRDPGGADARSRFVNPADAPLVQIPGTGVIWKISRDD"
     gene            2218..>2755
                     /locus_tag="VGO02_07150"
     CDS             2218..>2755
                     /locus_tag="VGO02_07150"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7477496.1"
                     /translation="MGRITTALLALLAAACPAAAAENGVSAGEIVIGQSASLTGGAAE
                     VGQQVRDGALAYFEMLNRSGGVNGRKVKLLSLDDGGQTKKGEENTAKLIEQDKVFLLF
                     GYTGRNTSEAALPVIDKAGVPFFGAATGGETIHGSFNKNVINVRASYKRETQVMVDYL
                     VTIGQKRVGMIYHKDDLTK"
BASE COUNT          440 a          929 c          952 g          434 t
ORIGIN      
        1 ggcttcctcg ccaacgagca ggcgcgctat gcgccgcagc tcgagcgcta cgccgcagcg
       61 ctcgacgcca cccgcctcgg gctctacttc cccgcgctcg ggcgctggcg ggactggaag
      121 aaataaaaag gggacaggcg ctgcgcctgt cccccgtgct gctccctgac tggttcagtc
      181 gtcccgcgag atcttccaga tcacgccggt gccggggatc tgcaccagtg gcgcgtcggc
      241 cgggttgacg aaacgcgagc gcgcgtcggc gccgcccgga tcgcgcaccg cgccgtagtc
      301 caccaggtac gccgcgccgt cggggccgaa gcggatggtg gtcgggcggt tgatgccgcg
      361 cagcatctgg gtgaacgcct ggtcggagtc gccgttgcag cgcggcgtgc cgtcagcgtc
      421 gtggcgctgc tcgctgcgcg cgcagttgaa cgcgaagcgc gagagcagca gctcggccgg
      481 gtcgtcgtcg ctggtgaagt tgaccagctc gacgtcgtgc cccgcgctcg ggttgccgtt
      541 ctcgggcgag aagccgaagt cgccttcgcg cgagaccagc accgcgtgct tcttcaccac
      601 gccgtgcacg aaggacttcg gcacgaagtc caggcccacc gccgccacgt cggccggctc
      661 gagcgccagc ggcgccaccg gcgggcgcgg cgggaacgcg aacaccggct tcaccgggac
      721 gtcctcggcg cggatgcgcg ccacgtctgg ccacaggtcg tcgcccgcgc cgccgatcgg
      781 gttgaacacc gcctgcgtcg actcgaggaa gccgaagcgg tcgggccagc cgtggtacga
      841 cggcgagccg tcggggttct gccgggcgag cgccaggcgg tcgggcgcat tgttgaccgg
      901 ccgcgcgccg cgctcgtcct cgccgttctc cgagacgaac agctcgcctt tcagcggatg
      961 gtcgctcggc gagaagcgca gcccgtaggg attgcggaag ccccacgcca ccggctcgat
     1021 gctgttggcc gggttggaga tcttcgcgcg caggatcgca ccggtgcaca tgccgcgccg
     1081 cgtcgcgcct tcgaacgccg gcacgaccgc gccggggcgc gccacgccgt ggttcgagta
     1141 gccgctggtg tggtgcccgt cacccgagtc ccaggtgttc tgcgacagct tcaccgtctc
     1201 gcaggcgatg tcgtgctggt tgccgccgcc gccgttgtca tgcccggtga cgccggagtt
     1261 ggtcgcggag ccctgcgacc agtacagcca gccgtccttc acgatgatct gctcggtcgg
     1321 gtggtcgccg gtcggcagcc ccgtgatcag cggcgtgacc gaaccgctcg agatattcac
     1381 ccggacgatg cgcgaggtgt tgttggccga gcccggcgcg ccgcgcaccc cctggttcga
     1441 gtcggtggcg aagagcgtgc cgccctcggt gtcgtgctcg aacgagaggc cgatcgccgg
     1501 gccgtcgggc tggaagccgc cgcccgaggg cgtgggcttg ccgagcgggc cggcgagctt
     1561 gcggccgttg cggtcgtaga tgaacaggcc gggcgtgaac gggttgcgcg gatcgaattt
     1621 gccgatgccg ggcacccggt tgttgtcgtt gcactggctg ggcagcccgg tgcccgactc
     1681 gaggaccagc acgcggaaat tgtgcttgtc gccgagaaag gcgacatcgg tcgggaagtt
     1741 gaggtccttg gcgaagacct cgaccttgta gccccgcggt accacgatgt cctcgccgtc
     1801 gcccgggttg taatacacac gctcttcggg acagaccggg ttcgacttat cggccagcgc
     1861 gggcgctggc gcgaatgcga gtccgacaag ggtaaataaa atggcgcgtt gcatgatcgc
     1921 ctcctctttg gtttcccccc tacgtcatct gttttcctct tgcgccgagc gggcgtcaat
     1981 ctcgcgcacc gactttcgtt gctcccgccg tccgaaaatc tgacgaagcc tccagccgcg
     2041 gctgggccaa ggtcgtcgag cagctcgagg ccacgctgct catcgctgcc gatgccggcg
     2101 tttcgggcat caaaccggga aaaaaattga aaaaccccgg cttatccagc gaaatatcac
     2161 tcgcgccggc cgactaaagg tcaacaatat cgcctgagtc caaaagggga gtcaggcatg
     2221 ggcaggatca ccacagcgct cctggcgctg ctcgccgccg cttgtccagc cgccgcggcc
     2281 gagaacggcg tcagcgccgg cgagatcgtc atcggccagt ccgcgtcgct caccggcggt
     2341 gcggcggaag tcgggcagca ggtgcgcgac ggcgcgctcg cctacttcga gatgctcaac
     2401 cgctcgggcg gcgtcaacgg acgcaaggtc aagctgctca gcctggacga cggcggccag
     2461 accaagaagg gcgaggagaa caccgccaag ctgatcgagc aggacaaggt gttcctgctg
     2521 ttcggctaca ccggccgcaa caccagcgag gcggcgctgc cggtcatcga caaggccggc
     2581 gtgccctttt tcggcgccgc gaccggcggc gagacgatcc acggcagctt caacaagaac
     2641 gtgatcaacg tgcgcgcgag ctacaagcgc gagacgcagg tgatggtcga ctacctcgtc
     2701 accatcggcc agaagagggt cggcatgatc taccacaagg acgacctgac caagt
//
LOCUS       DASYJA010000325         4077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2954148, whole genome shotgun sequence.
ACCESSION   DASYJA010000325 DASYJA010000000
VERSION     DASYJA010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2954148"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..979)
                     /locus_tag="VGO02_07155"
     CDS             complement(170..979)
                     /locus_tag="VGO02_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7477497.1"
                     /translation="MQQDHIVEIRDVSFGYDAAKPVLRDIDLAIPRGAVVGIMGQSGC
                     GKTTLLRMIMGALRPSAGTVRVLGRATADLRRDALYAMRREMGMLFQFGALFTDLSVF
                     ENVAFPLREHADLPDELLHDLVLLKLEAVGLRGAAHLMPAELSGGMARRVALARSIAL
                     DPHLILYDEPFTALDPISFGVISRLIRDLNGSLGATSLIVTHDVQEALGVVDYVYFMA
                     AGRIIAKGTPDEIRGSHEPFVNQFVNGKRDGPVPFQYPAPDYAADLELAAA"
     gene            998..1750
                     /locus_tag="VGO02_07160"
     CDS             998..1750
                     /locus_tag="VGO02_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7477498.1"
                     /translation="MRQGKEQGSGNAAVRIAALAKHYGRETALAGVDLEVARGEAFGL
                     VGANGAGKTTLIKCMLDLCAADGGEVEIFGMNARLFRARARLAYVPERFVPPHYLRCG
                     EFLQAAAALAGARYDAARAERLLAELDLDAGALGRPVRSLSKGMTQKLGLAAGFLVER
                     DLYVLDEPMSGLDPAARVAVKSVLRRLGAEGRTLFFTSHVLADVEELCSAMAVLDRGR
                     VRFRGSPAALKSRYGEADLEQAYMRCIRDQQS"
     gene            1734..3143
                     /locus_tag="VGO02_07165"
     CDS             1734..3143
                     /locus_tag="VGO02_07165"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEV7477499.1"
                     /translation="MTSSPSGKPALLVVDDDPLITDTLAFALGTDFEVLACDSRANAV
                     ELLRQLPTAPRVALVDLGLPPVPHTPDEGFALIGDLLAHAPSMKIFVLSGQNDAANAR
                     HARALGAFEFVAKPADPELLRKLLSRALEVPAGEEVDLVGSSAALAKLKNQIAQFAAA
                     PYPVLIEGESGSGKELVAANIHRLSPRSSRPYLTLNCAAISPTLVEPTLFGYVKGAFT
                     GAQSNKAGYFEDAQDGTLLLDEIGELPLELQAKLLRVLENGEYQRVGETQSRISRCRV
                     IAATNRDLRREVKKGAFRADLYHRLSVFTLGVPPLREMEGDKLALLEHFRRHYAARGG
                     AQPFELDEAAARRWQRYEFPGNVRELRNVVIRLATKYAGQRVSPAELEAELDLELDAD
                     PAAAPGAAPEGSILEQAARLLERGATFNLDETLKAWERAYIEAAMRLTRGNVSQAAKL
                     LGVNRTTLYSRMSSGEEKK"
     gene            3144..>4077
                     /locus_tag="VGO02_07170"
     CDS             3144..>4077
                     /locus_tag="VGO02_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7477500.1"
                     /translation="MALYLEHFGLTEPPFRITPHTDFFFEGADRGATLEALIYAVLHD
                     EGIVKVSGEVGSGKTMLCRVLMERLPESVEIIYLAVPSLSRDEMLAAIAGDLGIDTAG
                     ANTTKLVRALQDKLIAVHSAGRQVVALIDEAHAMPLGTLEEIRLLSNLETDKDKLLQI
                     VLFGQPELDVHLALPNMRQLKERITHAFTLGPLPQREIGEYVKFRLRAAGYHGPDLFG
                     PEALRIITEASEGLTRRINIYADKTLLAAFAAGTHTVSADHARAAVNDTQIVLQKRDS
                     SRRMATVALGGIAVGAVLGFLLAHVGSTPAPAPAV"
BASE COUNT          633 a         1467 c         1412 g          565 t
ORIGIN      
        1 tacacctcct tcaccgtcag gcggaagcgg cgcagcgcgg tacccgagtg cagcaccagg
       61 taggcgagga agcgggcggc gaagcccagg cgccagacgt gcgaggtcac ggcgccgccg
      121 aggcgctcga tgcgcgcgcg caagtccgcc cgcagatgcc cgctctccat caggctgcgg
      181 ccagctccag gtcggctgcg tagtcgggcg ccgggtactg gaacggcacc ggcccgtcgc
      241 gcttgccgtt gacgaactgg ttgacgaaag gctcgtggct gccgcggatc tcgtcgggcg
      301 tgcccttcgc gatgatgcgc ccggcggcca tgaagtagac gtagtcgacc acgccgagcg
      361 cctcctgcac gtcgtgggtg acgatgagcg aagtggcgcc gagcgagccg ttcagatccc
      421 ggatcaggcg cgagatgacg ccgaaggaga tcgggtcgag ggcggtgaac ggctcgtcgt
      481 acaggatcag atgcggatcc agggcgatgg aacgcgccag cgccacccgc cgcgccatgc
      541 cgccggagag ctcggccggc atgaggtgcg ccgcgccgcg caggcccacc gcctccagct
      601 tgagcaggac caggtcgtgc agcagctcgt cgggcagatc ggcatgctcg cgcagcggga
      661 aggcgacgtt ttcgaacacc gacaggtcgg tgaacagcgc gccgaactgg aacagcatgc
      721 ccatctcgcg ccgcatggca tagagcgcgt cgcgccgcag gtccgcggtg gcgcgcccca
      781 gcacgcgcac cgtcccggcg ctcgggcgca gcgcgcccat gatcatgcgc agcagcgtcg
      841 tcttgccgca gcccgactgc cccatgatgc cgaccacggc gccgcgcggg atcgccaggt
      901 cgatgtcgcg cagcaccggc ttggccgcgt cgtaaccgaa ggaaacatca cggatttcta
      961 cgatgtgatc ttgctgcacg gggttatatt atcccgcatg aggcagggta aagagcaggg
     1021 ctccggcaac gccgcggtgc gcattgccgc gctggccaaa cattacggcc gcgaaacggc
     1081 gctcgccggc gtcgacctcg aggtggcgcg gggcgaagct ttcgggctgg tcggggccaa
     1141 tggcgcgggc aagaccacgc tcatcaagtg catgctcgac ctgtgcgccg ccgacggcgg
     1201 cgaagtcgag atcttcggga tgaatgcgcg gctcttccgc gcgcgtgccc ggctggccta
     1261 cgtgccggag cgcttcgtgc cgccccacta tctgcgctgc ggcgagttcc tgcaggccgc
     1321 ggccgcgctc gccggcgccc gctacgatgc ggcgcgcgcc gagcggctgc tggccgagct
     1381 cgacctcgac gccggcgcgc tcggccggcc ggtgcgcagc ctgtcgaaag gcatgacgca
     1441 gaagctcggc ctcgccgccg gcttcctggt cgagcgcgat ctctacgtgc tcgacgagcc
     1501 gatgagcggc cttgaccccg ccgcccgggt cgcggtgaaa tccgtgctgc gccggctggg
     1561 ggccgagggc cggactctgt tcttcacttc ccacgtgctc gccgacgtgg aggagctatg
     1621 ctcggcgatg gcggtgctcg acaggggacg ggtccgcttc cgcggctcgc cggcggcgct
     1681 gaaaagccgc tacggcgagg ccgacctcga gcaggcctac atgaggtgca tccgtgacca
     1741 gcagtcctag cgggaaaccg gcgttgctgg tcgtcgacga cgacccgctc atcaccgaca
     1801 cgctggcgtt cgcgctgggc accgatttcg aggtccttgc ctgcgactcg cgcgccaacg
     1861 cggtcgagct gctgcgccag ctgccgaccg cgccgcgcgt cgcgctggtc gacctcggcc
     1921 tgccgcccgt gccccacacc cccgacgagg gcttcgcgct gatcggcgac ctgctggcgc
     1981 acgcgccgag catgaagatc ttcgtgctgt ccggccagaa cgacgccgcc aacgcgcgcc
     2041 acgcgcgagc gctgggcgcc ttcgagttcg tcgccaagcc cgccgacccg gagctcctga
     2101 ggaagctgct cagccgcgcg ctcgaggtac cggcgggcga ggaggtcgac ctggtgggct
     2161 cgagcgcagc gctcgccaag ctgaaaaacc agatcgcgca gttcgccgcc gcgccctacc
     2221 cggtgctgat cgagggcgag tcgggcagcg gcaaggagct ggtggcggcg aacatccatc
     2281 ggctgtcgcc gcgctccagc cggccttacc tcacgctcaa ctgcgccgcc atctcgccca
     2341 ccctggtcga gccgaccctg ttcggctacg tcaagggcgc gttcaccggg gcgcagtcca
     2401 acaaggccgg ctacttcgag gacgcgcagg acggcacgct gctgctcgac gagatcggcg
     2461 agttgcccct cgagctgcag gcgaagctgc tgcgggtcct cgagaacggc gagtaccagc
     2521 gcgtcggcga gacgcagagc cgcatctcgc gctgccgggt catcgccgcc accaaccgcg
     2581 acctgcgccg cgaggtcaag aagggcgcgt tccgcgccga cctctaccac cgcctgtcgg
     2641 tgttcacatt gggcgtgccg ccgctgcgcg aaatggaagg cgacaagctc gccctgctcg
     2701 agcacttccg gcgccactac gcggcgcgcg gcggggcgca gcctttcgag ctcgacgagg
     2761 ccgccgcgcg gcgctggcag cgctacgagt tcccgggcaa cgtgcgcgag ctgcgcaacg
     2821 tcgtcatccg cctcgccacc aagtacgccg ggcagcgggt ctcgcccgcc gagctcgagg
     2881 ccgagctcga cctggagctc gacgccgacc ccgcggccgc gcccggagcc gcgcccgagg
     2941 gctccatcct ggaacaggca gcgcgcctgc tcgagcgcgg cgctacgttc aacctcgacg
     3001 agacgctgaa ggcctgggag cgcgcctaca tcgaggcggc catgcgcctg acgcgcggca
     3061 acgtcagcca ggcggccaag ctgctcggcg tcaaccgcac cacgctctac agccggatga
     3121 gctcgggcga ggagaagaag taggtggcgc tctacctcga gcacttcggc ctgaccgagc
     3181 cgccgttccg catcacgccg cacaccgact tcttcttcga aggcgccgac cgcggcgcga
     3241 cgctcgaggc gctgatctac gcggtgctgc acgacgaggg catcgtcaag gtgtcgggcg
     3301 aggtcggcag cggcaagacc atgctctgcc gcgtgctgat ggagcgcctg ccggagtcgg
     3361 tggaaatcat ctatctcgcg gtgccgagcc tgtcgcgcga cgagatgctc gccgcgatcg
     3421 ccggcgacct cggcatcgac accgccggcg ccaacaccac gaagctggtg cgcgcgttgc
     3481 aggacaagct gatcgcggtg cattccgccg gccgccaggt ggtcgcgctc atcgacgagg
     3541 cgcacgcgat gccgctcggc accctcgagg agatccggct gctgtcgaac ctcgagaccg
     3601 acaaggacaa gctcctgcag atcgtcctct tcggccagcc cgagctcgac gtgcacctcg
     3661 cgctgccgaa catgcgccag ctcaaggagc ggatcacgca cgcgttcacg ctcggtccgc
     3721 tgccgcagcg cgagatcggc gagtacgtga agttccgcct gcgcgccgcg ggctaccacg
     3781 gccccgacct cttcggcccc gaggcgctgc gcatcatcac cgaggcctcc gaaggcctca
     3841 cgcgccgcat caacatctac gccgacaaga cgctcctcgc ggccttcgcg gcgggcaccc
     3901 acacggtgag cgcggaccac gcgcgcgccg ccgtgaacga tacccagatc gtgttgcaga
     3961 agcgcgactc ctcgcggcgc atggcgaccg tggccctcgg cgggatcgcg gtcggcgcgg
     4021 tgctgggatt cctgctggcg cacgtgggtt ccactccggc gccggcaccc gccgtcg
//
LOCUS       DASYJA010000326         2813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1591917, whole genome shotgun sequence.
ACCESSION   DASYJA010000326 DASYJA010000000
VERSION     DASYJA010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591917"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VGO02_07175"
     CDS             <1..640
                     /locus_tag="VGO02_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEV7477501.1"
                     /translation="LAAMSADRMVDRVEPGSYRLGMRLLELGNLVKTRISVRELALPL
                     MRELHAQTGETANLSVRHDDEIVYVERTSSGRSAMRVVHVIGARAPLHVTAAGKLFLL
                     EEGFARLRDYAKRTGLAPQTKNTIASPVALERELERIQRQGWATDNEEAEIGVRCVAA
                     GIRDDAGGLVAALSLSTPADRMKVQWGPLVKETADRVSRTIGHRPMARIGAA"
     gene            complement(637..1605)
                     /gene="egtD"
                     /locus_tag="VGO02_07180"
     CDS             complement(637..1605)
                     /gene="egtD"
                     /locus_tag="VGO02_07180"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEV7477502.1"
                     /translation="MGRDHRSRGNSGIDAAGLAAFRQDVIAGLSLPQKTLPPKHFYDA
                     AGSRLFERICRLPEYYLTRAELALTRAHLPAIARFAGSGGVLLEYGSGESLKTRLLLR
                     AMRPSVYMPVDISEDALRLAVARLGRAFPRLRIAPLAGDFSRPLDLPGFRGPDRRVVY
                     FPGSTIGNLSPQEAQAFLRMTRGQVGPRGAMLVGVDLKKDANLLHAAYNDARGVTAAF
                     NLNLLRRINRELGGDFDPKRFSHYAFYNAPLGRIEMHLVSLAAQSVNVGDHRFGFERG
                     ETIHTENSYKYSVEEFRAVAAESGFRGAKVWTDRRGLFSLHGLIAD"
     gene            complement(1566..2429)
                     /locus_tag="VGO02_07185"
     CDS             complement(1566..2429)
                     /locus_tag="VGO02_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV7477503.1"
                     /translation="MQRKLHPLEGIVAGFALVFVLALTIGFAQAGEQVKKVALRSNAV
                     LVVDAATGEVVMDKNGDAVTPIASITKLMTAIVILESGLDLQTPVALTREDVDLKKGS
                     RSRLRTGVSLTRDELLLIALMASENRAAAALGRSYPGGTQAFVAAMNEMSAELQMNDT
                     RFVDPTGLSPQNVSSARDLAKLVRAAHGYPLIREYSTRGKATVLLSGKQKMNYHNTNG
                     LVRNSRWEIDLSKTGYISEAGRCLVMQVRLASKDLIVVLLDSWGKHSRVGDANRIKKW
                     VETTAAAGIQG"
     gene            complement(2619..>2813)
                     /locus_tag="VGO02_07190"
     CDS             complement(2619..>2813)
                     /locus_tag="VGO02_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV7477504.1"
                     /translation="TLVIAHRLSTVMDADQILVLSHGRIVERATHQQLLELKGEYARM
                     WALQQQQAQAKAVLEEVIQA"
BASE COUNT          442 a          953 c          963 g          455 t
ORIGIN      
        1 cctcgccgcg atgagcgccg accgcatggt cgaccgcgtc gagccgggca gctaccgcct
       61 gggcatgcgg ctgctcgagc tcggcaacct agtgaaaact cgcatcagcg tacgcgagct
      121 cgccctgccg ctgatgcgcg agctgcacgc gcagaccggc gagaccgcca acctctcggt
      181 gcgccacgac gacgagatcg tgtacgtcga gcgcacgtcc tcgggccgct cggcgatgcg
      241 cgtggtgcac gtcatcggcg cgcgcgcgcc gctgcacgtc accgccgccg gcaagctgtt
      301 cctgctcgag gaaggcttcg cccgcctgcg cgactacgcc aagcgcaccg gcctcgcgcc
      361 gcagaccaag aacaccatcg ccagcccggt ggcgctcgag cgcgagctgg agcgcatcca
      421 gcgccagggc tgggcgaccg acaacgagga ggccgagatc ggcgtgcgct gcgtcgccgc
      481 cggcatccgc gacgacgccg gcggcctggt ggccgccctg tcgctctcca ccccggccga
      541 ccgcatgaag gtgcagtggg ggccgctggt caaggaaacc gccgaccgcg tttcacgcac
      601 gatcggccac cggccgatgg cgcgcatcgg cgctgcttag tcggctatca ggccgtgcag
      661 gctgaatagc ccgcgccggt cggtccagac tttcgcgccg cggaagcccg actcggcggc
      721 gacggcgcgg aactcctcga ccgagtattt gtacgaattc tccgtgtgga tggtctcgcc
      781 gcgctcgaag ccgaagcggt ggtcgccgac gttcacgctc tgcgcggcga gcgacaccag
      841 gtgcatctcg atgcggccga gcggcgcgtt gtagaacgca tagtgcgaga agcgcttcgg
      901 gtcgaagtcg ccgcccagct cgcggttgat ccggcgcagc aggttgaggt tgaacgccgc
      961 ggttacgccg cgcgcatcgt tgtaggccgc gtgcagcagg ttggcgtcct tcttcaggtc
     1021 cacgcccacc agcatcgcgc cgcgcgggcc gacctggccg cgcgtcatcc tgaggaacgc
     1081 ctgtgcctcc tgcggcgaga ggttgccgat ggtcgagccg gggaagtaga ccacgcgccg
     1141 gtcggggccg cgaaagcccg gcaggtcgag cggccgcgag aagtcgcccg ccagcggcgc
     1201 gatgcgcagc cgcggaaacg cccgcccgag cctggccacg gcgaggcgca gcgcgtcctc
     1261 cgagatgtcg accggcatgt acaccgaggg ccgcatggcg cgcagcagga gccgcgtctt
     1321 caggctctcg ccgctgccgt actcgagcag cacgccgccg ctaccggcga agcgcgcgat
     1381 ggccggaagg tgcgcgcggg tgagcgcgag ctcggcgcgc gtcaggtagt actcggggag
     1441 tcggcagatg cgctcgaaca ggcggctgcc ggccgcgtcg tagaagtgct tggggggcag
     1501 cgtcttctgc gggagcgaca gccccgcgat gacgtcctgc cggaacgcag ccagcccggc
     1561 cgcgtctatc cctgaattcc cgcggctgcg gtggtctcga cccacttttt gatgcggttg
     1621 gcgtcgccga cccgcgaatg cttgccccac gagtcgagca gcaccacgat caggtccttc
     1681 gacgcgaggc gcacctgcat cacgaggcag cgccccgctt cgctgatgta gccggtcttg
     1741 gacaggtcga tctcccagcg cgagttgcgc accaggccgt tggtgttgtg gtagttcatc
     1801 ttctgcttgc ccgagagcag caccgtcgcc ttgccgcgcg tcgagtattc gcggatcagc
     1861 ggatagccgt gcgccgcgcg caccagcttg gcgaggtcgc gcgcgctcga cacgttctgc
     1921 ggcgaaaggc cggtcgggtc gacgaagcgc gtgtcgttca tctgcagctc ggccgacatc
     1981 tcgttcatcg ccgcgacgaa ggcctgcgtg ccgcccgggt aggagcggcc cagcgcggcg
     2041 gcggcgcggt tctcggaggc catcagagcg atcagcagca gctcgtcgcg cgtcagcgag
     2101 acgccggtgc gcagccgcga gcgcgagccc ttcttcaggt cgacgtcctc gcgcgtcagc
     2161 gccacgggcg tctgcaggtc gaggccgctc tcgaggatca cgatcgcggt catcagcttg
     2221 gtgatcgagg cgatcggcgt caccgcgtcg ccattcttgt ccatcaccac ttcgccggtg
     2281 gcggcgtcga ccaccagcac ggcgttggag cgcagcgcca ctttcttcac ctgctcgccg
     2341 gcctgcgcga agccgatggt gagcgccagt acgaatacga gggcgaagcc ggcgacgatt
     2401 ccctcgagcg gatgaagctt tctttgcatt gcggcagttt ccctgagcga gctttttttc
     2461 gatgtgtcgc ctccatgcac tggttggagc agggaggaga ggtcgcagtt taaacgacag
     2521 ccgaaaaaaa ccgcaaggaa ataatgagct acaggcgttt tgaagaaact acgtgaatta
     2581 attcgctgtc gggcgccggc aacgctgccg gggcggctct atgcctgaat tacctcttcg
     2641 agcaccgcct tggcctgcgc ctgctgttgc tgcagcgccc acatcctggc gtattctcct
     2701 tttaactcaa gtagttgctg gtgcgtggca cgctccacga tgcgcccgtg gctgagcacc
     2761 aggatctggt cggcgtccat caccgtcgag agacggtggg cgatcactaa cgt
//
LOCUS       DASYJA010000327         1855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_504121, whole genome shotgun sequence.
ACCESSION   DASYJA010000327 DASYJA010000000
VERSION     DASYJA010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504121"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /gene="rng"
                     /locus_tag="VGO02_07195"
     CDS             complement(<1..1327)
                     /gene="rng"
                     /locus_tag="VGO02_07195"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEV7477505.1"
                     /translation="MSEEILINVTPQETRVAVVGAGAVQELLVERSASRGLVGNIYMG
                     KVARVLPGMQSAFVEIGLPRAAFLHVADIWEQREAAKPIEKILAEGQPVLVQVVKDPI
                     GTKGARLSTQVSIAGRLLVYLPHDPHIGISQRIEDESGRQALRDRLRELLPADEKGGF
                     IVRTLAESAGEDELRADLDYLRQIWRAIRERSGGCAGSSKPQLLYQDLSLAQRVLRDM
                     ASAGTVRVVIDSRENFQKLAAFAESYMPEVRAKIEHYSGERPLFDLYNVETEIERALS
                     RRVELKSGGTLIIDQTEAMTTIDVNTGGFVGSRSFDDTVFKTNLEAAQAIARQLRLRN
                     LGGIIIVDFIDMESDEHRAAVLEELKRTLARDRTRVTVNGFTTLGLVEMTRKRTRESL
                     AHVLCEPCPTCEGRGAVKTAHTVCYEILREILRESRAFKAKEFRVLASKA"
     gene            complement(1344..>1855)
                     /locus_tag="VGO02_07200"
     CDS             complement(1344..>1855)
                     /locus_tag="VGO02_07200"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEV7477506.1"
                     /translation="RPESKEEVNEDPLAGEAPAAYVERVARAKAESGWRRMMQRNLPQ
                     APVLAADTTVALGSRILGKPASREEAAEMLAALSGTRHEVLTAVALQQGEWLESALSV
                     SEVEFKKLTDEEIRQYVATGECDDKAGAYAIQGRAARFIIELRGSYTGVMGLPLYETS
                     QLIDKLPRR"
BASE COUNT          247 a          653 c          640 g          315 t
ORIGIN      
        1 ccgccttcga ggcgagcacg cggaactcct tcgccttgaa tgcgcgcgac tcgcgcagga
       61 tctcgcgcag gatctcgtag cacacggtgt gcgcggtctt caccgcgccg cggccctcgc
      121 aggtcgggca cggctcgcac agcacgtgcg ccagcgactc gcgggtgcgc ttgcgcgtca
      181 tctccaccag gccgagcgtg gtgaacccgt tcaccgtcac ccgggtgcgg tcccgcgcga
      241 gcgtgcgctt gagctcctcg aggaccgccg cgcgatgctc gtccgactcc atgtcgatga
      301 agtcgacgat gatgatgccg ccgaggttgc gcaggcgcag ctggcgcgcg atcgcctgcg
      361 ccgcctcgag gttggtcttg aacaccgtgt cgtcgaagct gcggctgccg acgaagccgc
      421 cggtgttgac gtcgatggtg gtcatcgcct cggtctggtc gatgatcagg gtaccgccgg
      481 atttcagctc gacgcggcgc gacagcgcgc gctcgatctc ggtctcgacg ttatagaggt
      541 cgaacagcgg ccgctcgccg ctgtagtgct cgatcttggc gcgcacctcc ggcatgtagc
      601 tctcggcgaa ggcggcgagc ttctggaagt tctcgcgcga gtcgatcacc acgcgcaccg
      661 tgcccgcgct cgccatgtcg cgcagcacgc gctgcgcgag cgacaggtcc tggtagagga
      721 gctgcggctt ggagctgccg gcgcagccgc ccgagcgctc gcggatcgcg cgccagatct
      781 gccgcaggta gtcgaggtcg gcgcgcagct cgtcctcgcc ggcgctttcg gcgagcgtgc
      841 gcacgatgaa gccgcccttc tcgtcggcgg gcagcagctc gcgcagccgg tcgcgcagcg
      901 cctgccggcc gctttcgtcc tcgatgcgct gcgagatgcc gatatgcggg tcgtgcggca
      961 ggtagaccag cagccgcccg gcgatcgaca cctgggtcga gagccgcgcg cctttcgtcc
     1021 cgatggggtc tttcaccacc tggaccagga ccggctggcc ctcggcgagg attttttcga
     1081 tgggcttggc ggcttcacgc tgctcccata tgtcggcgac atgaagaaac gccgcgcgcg
     1141 gcaggccgat ctcgacgaac gccgactgca tgccgggcag cacgcgcgcc accttcccca
     1201 tatagatatt gccgaccagg ccgcggctcg cggagcgctc gacgagcagc tcctgcaccg
     1261 cgccggcgcc gaccaccgcg acgcgcgttt cctgcggggt gacgttgatg aggatttcct
     1321 cgctcatggg atcgcgcttg cgctcagcgt cgcggcagct tgtcgatgag ctggctggtc
     1381 tcgtagagcg gcagccccat gacgccggta tagctgccgc gcagctcgat gatgaagcgc
     1441 gcggcgcgcc cctggatggc gtaggcgccg gccttgtcat cgcactcgcc ggtggcgacg
     1501 tactgtcgga tctcctcgtc ggtgagcttc ttgaactcga cctcggacac cgacagcgcg
     1561 ctctccagcc actcgccctg ctgcagcgcc accgcggtga gcacctcgtg gcgcgtgccg
     1621 gatagcgcgg cgagcatctc cgccgcttct tcgcggctgg ccggcttgcc gaggatgcgg
     1681 ctgcccaggg ccacggtggt gtcagcggcg agcaccggcg cctgcggcag gttgcgctgc
     1741 atcatgcggc gccagcccga ctcggccttg gcgcgcgcga cgcgctcgac gtaggcggcc
     1801 ggcgcctcgc cggcgagcgg atcctcgttg acctcctcct tcgactcggg acgcg
//
LOCUS       DASYJA010000328         2065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2046171, whole genome shotgun sequence.
ACCESSION   DASYJA010000328 DASYJA010000000
VERSION     DASYJA010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2046171"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VGO02_07205"
     CDS             <1..966
                     /locus_tag="VGO02_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HEV7477507.1"
                     /translation="RKKIIFGALIAAGGAAVAAVLLLPSQGGAPAAAGPKSAASRAQQ
                     PGAAGAERFAALPDREAVGRPAGQPFGSTRTPPPARAAAAAPVAAAPAAPPMPYRIAG
                     QMVQGGAAQVVLAKGDRVLTVREGDTLEDGYRVEAIGPQGVTLLYVPMNIRETLPFVS
                     ALGLDAPAAQPPAQSAQPPAQPAAQPGSEARPAQLRWEGPERVQAGSTFNVALRVTSD
                     QPVRASPLQLSYDAALLEPVDVRAGGFFSGGSFSYRINPGGSILVGAFGPGDVAADAE
                     FLVVTFRPIRAGATAELKLSSVLLQGAAGRAIVHAQPAAFRTAIQ"
     gene            complement(971..1888)
                     /locus_tag="VGO02_07210"
     CDS             complement(971..1888)
                     /locus_tag="VGO02_07210"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV7477508.1"
                     /translation="MNITSRQLKAFLLTARHQSFSRAAERLFITQSGMSVLVRELEDQ
                     LGFRLFDRTTRRVTLTEHGTKFLPIADRSLLDLESAAANIGRSATAADRHLSLGAPPL
                     IAGKLLAAAIGEYAKRDPELRISLHDGERGKLISMVAAGTLDVGVGCFLQPVTGVRRT
                     PVYRFSLMLLEPAAHAGEAPQPMPWSEIGERPLLTLAADHPVQQVIDLHLQSIGRRAA
                     PHMVFSYFDTQIAMVEAGAGSAVLPTFVLPACLERRVAMHPLTDPVVPIELYQIVNRG
                     KKLPAAAEGFTRFLRSYIAEWAEPWNPGR"
BASE COUNT          305 a          718 c          736 g          306 t
ORIGIN      
        1 cgcaagaaga tcatcttcgg tgcgttgatc gcggccggcg gcgctgccgt cgcggctgtg
       61 ctgctgctgc cgtcgcaagg cggcgcaccc gccgcggcgg ggccgaaatc cgcggcgagc
      121 cgcgcgcagc agcctggcgc agcgggcgcg gagcgcttcg ccgcgctgcc ggatcgcgag
      181 gccgtgggcc gcccggcggg ccagcctttc ggctctaccc ggacgccgcc gcctgcgcgc
      241 gccgccgctg cggcgccggt cgccgccgcg cccgccgcgc cgccgatgcc ctaccgcatc
      301 gccggccaga tggtgcaagg cggcgcagcg caggtcgtgc tggcgaaagg cgaccgcgtc
      361 ctcaccgtgc gcgaaggcga cacgctcgag gacggctatc gcgtcgaggc gatcgggccg
      421 cagggcgtca cgctgctcta cgttccgatg aatatccgcg agacgctgcc gttcgtctcg
      481 gcgctcggcc tcgacgcgcc ggccgcgcag ccgccggcgc agtccgcgca gccaccggcg
      541 cagcctgccg cacagcccgg ttcagaagcg cgaccggccc agctgcgctg ggaagggccc
      601 gagcgggtgc aggccggcag caccttcaat gtcgcgctca gggttacctc ggaccagccg
      661 gtgcgcgcct cgccgctgca gctcagctac gacgccgcgc tgctcgagcc ggtcgacgtg
      721 cgcgccggag gtttcttcag cggtggcagc ttcagctacc gcatcaatcc cggcggctcg
      781 atcctggtcg gggcgttcgg ccccggcgac gtggccgccg acgcggagtt cctggtcgtc
      841 acgttccggc cgatccgcgc cggcgcgacc gccgagctga agctgtcctc ggtgctgctg
      901 cagggcgcgg ccggtcgcgc catcgtgcac gctcagccgg cggcgtttcg caccgctatc
      961 cagtaagtaa ctagcgcccc ggattccagg gctcggccca ctccgcgatg tagctgcgca
     1021 ggaagcgcgt gaacccctcc gcggcggcgg gcagcttctt gccgcgattg acgatctggt
     1081 agagctcgat cggcaccacc gggtcggtga gcggatgcat cgcgacgcgc cgctcgaggc
     1141 aggccggcag cacgaaggtg ggcagcaccg cgctgccggc gccggcttcc accatcgcga
     1201 tctgcgtgtc gaagtagctg aacaccatgt gcggcgcggc gcgccgaccg atgctctgca
     1261 gatgcaggtc gatgacctgc tgcaccgggt ggtcggccgc cagcgtgagc agcggccgct
     1321 cgccgatctc cgaccagggc atcggctgcg gcgcttcgcc ggcgtgcgcc gccggctcga
     1381 gcagcatcag ggagaagcgg tagacgggcg tgcggcgcac gccggtcacc ggctgcagga
     1441 agcagcccac gcctacatcg agcgtacccg ccgccaccat cgagatcagc ttgccgcgct
     1501 cgccgtcgtg caggctgatg cgcagctccg gatcgcgctt cgcgtactcg ccgatcgccg
     1561 ccgccagcag cttgccggcg atcagcggcg gcgcgccgag cgagagatgg cggtcggccg
     1621 cggtggcgga gcggccgatg ttggccgccg ccgattcaag gtcgagcagg ctgcggtcgg
     1681 cgatcggcag gaacttggtg ccgtgctcgg tgagggtcac gcgccgcgtg gtgcggtcga
     1741 agaggcgaaa gccgagctgg tcctccagct cgcgcaccag cacgctcatt ccggattgcg
     1801 tgatgaacag gcgctcggcg gcgcgcgaga agctctggtg gcgcgccgtc agcaggaagg
     1861 ccttcaattg acgggaggtg atattcatgg ctagaagcga ttaattcgag cgttagttca
     1921 tttcagcttc ttgatcaccg ggcccaatcc tatcaagggc tgacaatttt tgacgatccc
     1981 ggtcgaaaca aaaaaaggaa agaattaccg gcctggtttg ctgcaaagca gcagccgcgc
     2041 cagggatcca tattgcgggt atata
//
LOCUS       DASYJA010000329         5266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1319420, whole genome shotgun sequence.
ACCESSION   DASYJA010000329 DASYJA010000000
VERSION     DASYJA010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319420"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VGO02_07215"
     CDS             complement(<1..431)
                     /locus_tag="VGO02_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477509.1"
                     /translation="MDKVLALRGGGEAVALWAQLASVVDLVAGIALAGIGGGLTVLVA
                     QAAEPERRREFLRQALSVGLVLSLAPALGVLLGAALLETGMPRLTVGGAALAGWIVVI
                     PGLLNSYWLGRERRDAQLALAAASALISVAAVAWAPSSWML"
     gene            complement(504..1190)
                     /locus_tag="VGO02_07220"
     CDS             complement(504..1190)
                     /locus_tag="VGO02_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HEV7477510.1"
                     /translation="MRSVIFPGFAAAVMLFAAPAIAQLDQITSREAVAGLKAALEKGS
                     QAAVAALGRVDGFFGNPQVKIPLPESLQRTERLMRRVGMAKYADELILTLNRAAESAV
                     PGARQLLLDSVRKMTVQDAKGILSGGDTAATEYFRRTTQDALRKRFLPIVKQATSRVE
                     LAEKYNEYADTAVAFGLVKKDDANLDDYVTQKALDGLFFMVAEEEKAIRKDPVGSASA
                     ILRKVFGALR"
     gene            1189..1689
                     /locus_tag="VGO02_07225"
     CDS             1189..1689
                     /locus_tag="VGO02_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEV7477511.1"
                     /translation="MKTAIIVGLAALALGVAASFALQQRQASAPEVRFATLSGDSFAT
                     SDLRGKVVLVNFWATSCVSCVQEMPKMIETYKKFAPRGYEMVAVAMSYDHPNQVAQFA
                     KDRALPFKVALDSNGAIAKSFGDIRITPTTFVLDRQGRVLKRYLGEPDWAEFHGLLDK
                     ALRDPA"
     gene            complement(1657..2532)
                     /locus_tag="VGO02_07230"
     CDS             complement(1657..2532)
                     /locus_tag="VGO02_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEV7477512.1"
                     /translation="MNTTLVINSKGGSGKTTITTTLASYFAVHGEPTAVLDYDLQGSS
                     LNWLRVRDPLAPKIHGANAAPQKVGLRSFGRHVPEGTRQLIIDAPAGASGLLLQDMLS
                     RANCIVIPVQPSAIDIHATANFVRDLLLRGNIRARNIRLAVVANRVRKATPVYQPLER
                     FLTSLGLTIVTRLSDSEAFVKAAETGLGIFEMDASQYMAEREQCQPLIEWVDVGHKVR
                     TTSTVIEMPRVALAGRGQSEVRRDLVRTMPPRPALPPRPLLPAKAALLAKPQPAQPKA
                     QLPAWLSRISQRFVE"
     gene            complement(2598..3104)
                     /locus_tag="VGO02_07235"
     CDS             complement(2598..3104)
                     /locus_tag="VGO02_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV7477513.1"
                     /translation="MQTSKPTKPASPEAIRAADAAPKKKQTNYPEPFASRVAGREKRP
                     LGDVFGLSNFGVNLTRLKPGAVSSVRHAHTRQDEFVYVLDGHPTLVTDAGETVLAPGM
                     CAGFKAGTGNGHHLVNRSPSDVVILEVGDRSAGDAATYPDDDLKAVQAPAGGWIFTRK
                     DGTPFQEK"
     gene            complement(3067..3996)
                     /gene="lipA"
                     /locus_tag="VGO02_07240"
     CDS             complement(3067..3996)
                     /gene="lipA"
                     /locus_tag="VGO02_07240"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEV7477514.1"
                     /translation="MREAGVKEKGARKTIRIVPQTPLPKPPWIRVRAASADSRFGEIK
                     RILREQKLHTVCEEASCPNIAECFGKGTATFMILGDICTRRCPFCDVAHGRPLAPDAE
                     EPRHLAETIAALKLSYVVVTSVDRDDLKDGGARHFVDCINAIRETSPRTTIEVLVPDF
                     RGRMERALEILAAAPPDVMNHNLETVPRLYRKARPGGDYAHSLLLLKNFKKLYPAVPT
                     KSGLMVGLGETDEEILAVMRDLRAHDVDMLTIGQYLQPSPHHLPVERYVDPAVFSMYE
                     TEASGMQFRHAAIGPMVRSSYHADQQAHQAGVA"
     gene            complement(3989..4600)
                     /gene="lipB"
                     /locus_tag="VGO02_07245"
     CDS             complement(3989..4600)
                     /gene="lipB"
                     /locus_tag="VGO02_07245"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010922.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEV7477515.1"
                     /translation="MSAVLERIVVKQLGQIEYQAALRAMKSFSSQRKEATPDELWLVE
                     HPAVYTLGQGAKEVVVSNGIPLVKSDRGGEITYHGPGQVVLYALIDLARRGIKVKQFV
                     ALLEQSVIDLVGPRAERKAGAPGVYVGGAKLAALGIRVARGRSYHGLALNVDMDLAPF
                     GAIDPCGFPGLAVTQMKHLGFSMSMEETGKRLAGLLAEGIENA"
     gene            complement(4597..4809)
                     /locus_tag="VGO02_07250"
     CDS             complement(4597..4809)
                     /locus_tag="VGO02_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HEV7477516.1"
                     /translation="MGRKERGFAQNMTEIVLKHAPDFDVASVEMRPSRQGKYLSITCV
                     VRAVSREQLDSLYQELCDHPSVVMVL"
     gene            complement(4850..>5266)
                     /locus_tag="VGO02_07255"
     CDS             complement(4850..>5266)
                     /locus_tag="VGO02_07255"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEV7477517.1"
                     /translation="VLLRQLSAEADAAETILFRDGKLTEASASNVFVVKGGVIQSPPK
                     SNLILSGITYDVIAELAAANALPMEFRDVTEAEVRGADEVWVSSSSKEVFPIVTLDGR
                     QIGDGKPGPVFRRMYQLYQDFKHTVMRSGKREAISA"
BASE COUNT          852 a         1758 c         1747 g          909 t
ORIGIN      
        1 tcgagcatcc agctggacgg cgcccacgca accgccgcca ccgaaatcaa cgccgatgcg
       61 gcggcgagcg cgagctgcgc gtcgcgtcgc tcgcggccga gccagtagct gttgagcagt
      121 cccgggatga ccacgatcca gccggcgagc gcggcgccgc cgaccgtgag ccgcggcatg
      181 ccggtctcga gaagagctgc tccgagcagc acgccgagcg ccggcgcgag cgacagcacc
      241 aggccgacgc tcagcgcctg gcgcaggaac tcgcggcgcc gctcgggctc ggctgcctgc
      301 gcgaccagca cggtgaggcc gccgccgatg ccggcgagag cgatgcccgc caccaggtcg
      361 acgaccgacg cgagctgcgc ccagagcgcc accgcctcgc cgccgccgcg cagggcgagc
      421 accttgtcca ccgccgagcg gccgaccagg ccggcggcga tgatggcgag cgtgagggcg
      481 agcttgatgg gctagccgaa tctctaccgc agggcgccga agactttgcg aaggatggcc
      541 gaggcgctgc cgacgggatc cttgcggatc gccttctcct cctccgccac catgaagaac
      601 aggccgtcga gcgccttttg cgtcacgtag tcgtccaggt tggcgtcgtc cttcttcacc
      661 agaccgaaag cgaccgcggt gtccgcgtat tcgttgtact tctccgccag ctcgacccgc
      721 gaggtggcct gcttcacgat gggcaggaag cgcttgcgca gcgcgtcctg cgtggtgcgc
      781 cggaagtatt cggtggcggc cgtgtcgccg cccgagagga tgcctttcgc gtcctgcacc
      841 gtcatctttc tcaccgagtc gaggaggagc tggcgcgccc cgggcaccgc gctttcagcc
      901 gcgcggttga gggtgaggat cagctcgtcg gcgtacttcg ccattccgac gcggcgcatc
      961 aaccgctcgg tcctttgcag cgactccggc agcgggatct tcacctgcgg attgccgaag
     1021 aagccgtcga ccctgcccag agcggccact gccgcctggg agcctttctc gagcgccgcc
     1081 ttcagcccgg cgacagcttc gcggctggtg atctggtcca gttgggcgat agcgggtgcc
     1141 gcgaacaaca tgacagcggc ggcgaacccg gggaaaatca cgcttctcat gaagacagcc
     1201 attatcgtcg gcctcgcggc gctcgccctc ggcgtggccg cctcattcgc gctccagcag
     1261 cggcaagcga gcgcccccga agtgcgcttc gcgacgctgt cgggcgacag cttcgccact
     1321 tccgacctgc gcggcaaggt ggtgctggtg aatttctggg ccacctcctg cgtcagctgc
     1381 gtgcaggaga tgccgaagat gatcgagacg tacaagaagt tcgcgccgcg cggctacgag
     1441 atggtcgcgg tggccatgag ctacgaccat cccaaccagg tggcgcagtt cgcgaaggat
     1501 cgcgccctgc ccttcaaggt ggcgctcgac agcaacggcg ccatcgcgaa aagcttcggc
     1561 gacatccgca tcacgccgac cacgttcgtc ctcgaccggc aggggcgggt gctgaagcgc
     1621 tacctaggcg agcccgactg ggccgagttc cacggcctac tcgacaaagc gctgcgagat
     1681 ccggcttagc caggcgggca actgtgcctt gggctgcgcc ggttgcggct tcgccagcag
     1741 cgcggccttc gccggcagca gcggcctggg cggcagcgcc ggcctgggcg gcatggtgcg
     1801 caccagatct cgcctcacct cagactgacc ccgtccggcg agcgcgaccc gcggcatctc
     1861 gatcaccgtg ctcgtcgtgc gcaccttgtg ccccacgtcg acccattcga tgagcggctg
     1921 gcactgctcg cgctcggcca tgtactgcga cgcgtccatc tcgaaaatgc cgagcccggt
     1981 ttcggccgcc ttgacgaagg cctccgagtc gctcagccgc gtcacgatgg tcagcccgag
     2041 cgaggtgagg aagcgttcca gcggctggta caccggcgtc gccttgcgca cgcggttggc
     2101 gaccactgcg aggcggatgt tgcgcgcgcg gatgttgccg cgaagcagca gatcgcgcac
     2161 gaaattggcg gtggcgtgga tgtcgatcgc cgagggctgc accgggatca cgatgcagtt
     2221 cgccctcgac agcatgtcct gcagcagcag accagaggcg cccgccggtg cgtcgatgat
     2281 gagctgccgg gtgccctccg gcacgtggcg gccgaagctg cgcagaccca ctttctgcgg
     2341 cgcggcgttg gcgccgtgga tcttcggcgc gagcggatcg cgcacgcgca gccagttgag
     2401 ggaggagccc tgcaggtcat agtccagcac cgcggtcggc tcgccgtgga cggcgaaata
     2461 gctggcaagg gtggtcgtga tcgtggtctt gcccgacccg cccttcgagt tgatcaccag
     2521 cgtggtgttc acccgcgctt cccctctccc ggtacgacct tcacagggta cccaagaaat
     2581 ccccattcgc atgttaacta cttctcctgg aacggtgttc cgtccttgcg ggtgaagatc
     2641 cacccccctg cgggggcctg aacggccttc agatcgtcgt cagggtaggt cgccgcgtcg
     2701 ccggccgagc ggtcgccgac ctccaggatg accacgtccg acggcgaccg gttgaccagg
     2761 tggtgcccgt tccccgtccc ggccttgaac ccggcgcaca ttccaggtgc aagaacggtc
     2821 tcgccggcat cggtcaccag ggtgggatgg ccatcgagca cgtagacgaa ctcgtcctgc
     2881 ctggtgtgcg catggcgcac cgacgagacg gcccccggct tgaggcgggt caggttgacg
     2941 ccgaaattgg acaatccgaa tacatcgccg agggggcgct tctcccgccc ggcgacgcgc
     3001 gaggcgaacg gctcgggata gttggtctgc ttcttcttcg gcgcggcgtc ggccgcgcgg
     3061 atcgcttcag gcgacgccgg cttggtgggc ttgctggtct gcatggtagg aggatcgcac
     3121 catcggaccg atcgcggcgt gtctgaactg catcccggat gcttcagttt cgtacatcga
     3181 gaaaaccgcc ggatcgacat agcgctcgac cggcagatgg tgcggcgagg gctgcaggta
     3241 ctggccgatg gtgagcatgt cgacgtcgtg cgcgcgcagg tcgcgcatca cggcgaggat
     3301 ttcctcgtcg gtctcgccca ggccgaccat caggcccgac ttggtcggca ctgcggggta
     3361 cagcttcttg aaatttttca acaacaaaag agaatgagcg taatccccgc ccgggcgcgc
     3421 cttcctgtag aggcgcggca cggtctctag gttgtgattc attacgtccg gcggcgcagc
     3481 ggccaggatc tccagcgcgc gttccatccg gccgcggaag tcgggcacga gaacctcgat
     3541 cgtcgtgcgc ggcgaggttt ctcttattgc attgatgcag tcgacgaagt gccgggcgcc
     3601 gccgtccttc aggtcatcgc ggtcgacgct cgtcaccacc acgtaggaga gcttgagcgc
     3661 ggcgatggtc tcggcgaggt gacgcggctc ctcggcgtcg ggcgcgagcg gccggccgtg
     3721 cgccacgtcg cagaagggac agcggcgcgt gcagatgtcg cccagaatca tgaaggtcgc
     3781 ggtgcccttg ccgaagcact cggcgatgtt ggggcacgag gcttcctcgc acacggtgtg
     3841 cagcttctgc tcgcgcagga tccgcttgat ctcgccgaag cgcgagtcgg cgctcgccgc
     3901 acgcacacgg atccaaggcg gcttgggcag cggcgtctgc gggacgatgc ggatcgtctt
     3961 cctcgcgccc ttttccttta cgcctgcttc acgcattttc gattccttcg gcaagaagtc
     4021 ctgccagccg ttttccggtt tcttccattg acattgaaaa tccgagatgt ttcatctgcg
     4081 ttaccgccag gcccggaaag ccgcaagggt cgattgcgcc gaacggcgcg aggtccatgt
     4141 cgacattgag cgcgaggccg tggtagctgc ggccgcgcgc gacgcggatg ccgagcgcgg
     4201 cgagcttggc gccgccgaca tagacgccgg gcgcgccggc cttgcgctcg gcgcgcgggc
     4261 cgacgaggtc gatcaccgac tgctcgagta acgccacgaa ttgcttcacc ttgatgccgc
     4321 ggcgcgcgag gtcgatcagc gcgtacagca cgacctgccc gggtccgtgg taggtgatct
     4381 cgccgccgcg gtccgacttt acgagcggga tgccattgct caccactacc tctttcgccc
     4441 cctgccccag cgtatacacc gccgggtgct caaccagcca caactcatcc ggcgtggctt
     4501 cttttcgttg ggaagaaaat gatttcatgg cacgcaatgc cgcctggtac tcgatttgcc
     4561 caagctgctt gacgacgatc cgttcgagaa cggcgctcac agcaccatca ccacggaagg
     4621 atggtcgcac agctcctggt agagcgaatc gagctgctcg cgcgagacgg cgcgcaccac
     4681 gcaggtgatg ctcaggtact tgccctgccg gctgggacgc atttccacgc tggccacatc
     4741 gaagtccggc gcgtgcttga ggacgatctc ggtcatgttc tgggcgaagc cgcgttcttt
     4801 cctgcccatg atcttgattg gaaagtcgca gggaaaggct aacgccgact caggcagaga
     4861 tcgcctcgcg cttgcctgag cgcattaccg tgtgcttgaa gtcctgatag agctgataca
     4921 tgcgccggaa taccgggccc ggcttgccgt cgcctatctg ccggccgtcc agcgtgacga
     4981 taggaaacac ttccttcgac gaggagctga cccaaacctc gtccgcgccg cgcacttcgg
     5041 cctcggtgac atccctgaat tccatcggca gagcgttggc ggcggcgagc tcggcgatca
     5101 cgtcgtaggt gatgccggac aggatcaggt tgctcttcgg cggcgactgg atcacgccgc
     5161 ccttgacgac gaacacgttc gaggcggacg cctcggtgag cttgccgtcg cggaacagga
     5221 tcgtctcggc cgcgtcggcc tcggccgaga gctggcgcag cagcac
//
LOCUS       DASYJA010000330         2931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1410859, whole genome shotgun sequence.
ACCESSION   DASYJA010000330 DASYJA010000000
VERSION     DASYJA010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410859"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..1391)
                     /gene="dnaJ"
                     /locus_tag="VGO02_07260"
     CDS             complement(282..1391)
                     /gene="dnaJ"
                     /locus_tag="VGO02_07260"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006417099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEV7477518.1"
                     /translation="MAKRDYYEVLGVNRDAAEEDLKKAYRRLAMKWHPDRNPDNPKAE
                     EHFKEAKEAYEILSDANKRAAYDQFGHAGVDPQAGAGGMGGFGDIFSDIFGEIFGGGR
                     GGRSTVYRGADLRYNLEITLENAAHGMDTKIRIPTAAVCETCKGSGARAGTQPITCPA
                     CRGAGQVRVSQGPFSIAQTCPRCHGTGSVIANPCNACHGTGRIKLQKTLSVRIPAGVD
                     EGDRVRLSGEGEPGVNGGPPGDLYVQVHIKPHAVFQRNHDDLHCEMPISITTAALGGE
                     IEIPTLDGSARIRVSAETQSGKQFRLKGKGIKGVRSQAPGDLFCHVVVETPVQLTERQ
                     RQLLREFESISNADSARHNPRAKGWLEKVKEFFEG"
     gene            complement(1423..>2931)
                     /gene="dnaK"
                     /locus_tag="VGO02_07265"
     CDS             complement(1423..>2931)
                     /gene="dnaK"
                     /locus_tag="VGO02_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEV7477519.1"
                     /translation="YFNDAQRQATKDAGRIAGLEVKRIINEPTAGALAFGMDKQTKKD
                     EKIAVYDLGGGTFDISAIEIADVEGEKQFEVLATNGDTFLGGEDFDQRLIDYVVGEFK
                     KEQGVDLAKDVLALQRLKETAEKAKIELSSSQQTELNLPYITADQSGPKHLTMKITRA
                     KFESLVDDLIEKTIGPCRIAVKDAGLSVQQIHDVILVGGQTRMPKVQDKVKEFFGREP
                     RKDVNPDEAVAIGAAIQAGVLQGDVKDVLLLDVTPLSLGIETLGGVTTKLIQKNTTIP
                     TKAQQVFSTADDNQNAVTIHVLQGERDMASGNKSLGQFNLTDIPPAPRGMPQIEVTFD
                     IDANGILHVTARDKATGKENKIKIQASSGLNEAEIQRMVKDAEVHAEEDRKTLELINA
                     RNQAEATVHSVKKSLKDYGDKLDAGEKEKIEAALKDAEGVLKSEDKAEIEAKAQALAQ
                     AAQKLGEKMYAEEQAKQQAQQPGDAQAADAGKKDDGNVVDAEFTEVKDKKTG"
BASE COUNT          371 a          981 c          965 g          614 t
ORIGIN      
        1 aagtcgagtc cgcgggcgcg ccgaggctga tggattggat gacgccgacg ccgggcttcc
       61 aggccgaaga ggaccccttc ggcgcgggaa cgctcgaacc cgcgctcgct acgggagcgc
      121 cgttcgtcgt cctgacggcc accggtggag gcggatcgta ataggtcgtg cagcccgcga
      181 ttccgagggc ggcgagcgtg cagagggtca gcgtgaggcg actcatggcg agctccggat
      241 caatgaaggc gcgttgatcc gagcaattca cgtgcccacg actatccctc gaagaactcc
      301 ttgaccttct cgagccagcc cttggcgcgc gggttgtgac gcgcgctgtc tgcgttgctg
      361 atcgactcga actcgcgcag cagctggcgc tggcgctcgg tgagctgcac cggcgtctcc
      421 acgaccacat ggcagaagag gtcgcccggc gcctggctgc gcacgccctt gattcccttg
      481 cccttcaagc ggaactgctt gccgctctgg gtttcggccg acacgcggat gcgcgccgag
      541 ccgtcgaggg tcgggatctc gatctccccg ccgagcgccg cggtggtgat cgaaatcggc
      601 atctcgcagt gcagatcgtc gtggttgcgc tggaataccg cgtgcggctt gatgtgcacc
      661 tgcacgtaga ggtcgcccgg cggtccgccg ttcacgcccg gctcgccctc gcccgagagc
      721 cgcacgcggt cgccttcgtc gacgcccgcg ggaatcctga ccgagagcgt cttctgcagc
      781 ttgatgcggc cggtcccgtg gcaggcgttg cagggattgg cgatgacgct gccggtgccg
      841 tggcagcgcg gacaggtctg ggcgatcgaa aaaggcccct gggaaacacg cacctggccg
      901 gcgccgcggc acgccgggca ggtgatgggc tgcgttccgg cgcgtgcgcc gctacccttg
      961 caggtctcgc acacggccgc ggtcgggatg cggatcttgg tgtccatgcc gtgcgccgcg
     1021 ttctcgaggg tgatctcgag gttgtagcgc aggtccgcgc cgcggtacac ggtcgagcgc
     1081 ccgccgcggc cgccgccgaa gatctcgccg aagatgtcgc tgaagatgtc gccgaagccg
     1141 cccatgccgc cggcgcccgc ctgcgggtcg acgccggcgt ggccgaactg atcgtaggcc
     1201 gcgcgcttgt tggcgtcgga aagaatctcg taggcttcct tcgcctcctt gaagtgctcc
     1261 tcggccttgg ggttgtccgg gttgcggtcc gggtgccact tcatggcgag acgccggtag
     1321 gccttcttca ggtcctcctc ggctgcatcc cggttcactc cgagcacttc gtagtagtcg
     1381 cgcttcgcca tgctcccgtt cttgttttac ctacttgaaa aactatccgg tcttcttgtc
     1441 cttgacctcg gtgaactcgg cgtcgacgac gttaccgtcg tccttcttgc cggcgtcggc
     1501 ggcctgcgcg tcaccgggct gctgcgcctg ctgcttggcc tgctcctcgg cgtacatctt
     1561 ctcgcccagc ttctgcgcgg cctgcgccag cgcctgcgcc ttcgcctcga tctcggcctt
     1621 gtcctccgac ttgagcacgc cctcggcgtc cttcagcgcg gcctcgatct tctccttctc
     1681 gcccgcgtcg agcttgtcgc cgtagtcctt cagcgacttc ttcaccgagt gcaccgtcgc
     1741 ctcggcctgg ttgcgcgcgt tgatcagctc gagcgtcttg cggtcttcct cggcgtgcac
     1801 ctcggcgtcc ttcaccatgc gctggatctc ggcctcgttc aggcccgagc tcgcctggat
     1861 cttgatcttg ttctccttgc cggtagcctt gtcccgggcg gtgacgtgca ggatgccgtt
     1921 ggcgtcgatg tcgaaggtga cctcgatctg cggcatgccg cgcggcgccg gcgggatgtc
     1981 ggtgaggttg aactgcccca gcgacttgtt gcccgaggcc atgtcgcgct cgccctgcag
     2041 cacgtggatg gtcaccgcgt tctggttgtc gtcggcggtg gagaatacct gctgcgcctt
     2101 ggtcgggatg gtggtgtttt tctgaatcag cttggtcgtc accccgccca gtgtctcgat
     2161 gcccagcgac agcggcgtga cgtcgagcag cagcacgtcc ttgacgtcgc cctgcagcac
     2221 gccggcctgg atcgcggcgc cgatggcgac cgcctcgtcc gggttgacgt ccttgcgcgg
     2281 ctcgcggccg aagaattcct tcaccttgtc ctgcaccttc ggcatgcgcg tctggccgcc
     2341 gaccaggatc acgtcgtgga tctgctgcac ggagaggccc gcgtccttca ccgcgatgcg
     2401 gcagggcccg atggtcttct cgatgagatc gtccaccagc gactcgaact tggcgcgggt
     2461 gatcttcatc gtcaggtgct tcgggccgct ctggtcggcg gtgatgtagg gcaggttgag
     2521 ctcggtctgc tgcgaggagg acagctcgat cttcgccttc tccgccgttt ccttcagccg
     2581 ctgcagcgcg agcacgtcct tggcgagatc gacgccctgt tccttcttga actcgccgac
     2641 cacgtagtcg atcaggcgct ggtcgaagtc ttcgccgccg aggaacgtgt cgccgttggt
     2701 ggctagtact tcgaactgct tctcgccctc gacatcggcg atctcgatgg cggagatgtc
     2761 gaaggtgccg ccgcccaggt catagacggc gatcttctcg tccttcttgg tctgcttgtc
     2821 catgccgaag gcgagcgcgc ccgcggtcgg ctcgttgatg atgcgcttca cttcaaggcc
     2881 ggcgatgcgg cccgcgtcct tggtcgcctg gcgctgcgcg tcgttgaagt a
//
LOCUS       DASYJA010000331         6566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2592044, whole genome shotgun sequence.
ACCESSION   DASYJA010000331 DASYJA010000000
VERSION     DASYJA010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6566
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2592044"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="VGO02_07270"
     CDS             complement(<1..1153)
                     /locus_tag="VGO02_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEV7477520.1"
                     /translation="MNKIFPAILAVLLAACATPPAAPPDVASPAAAAPQDSAFPTPFP
                     TQAPAPTPGGTGIVDASKLPPFDGEQRSIKDPLRGLAKLDHTVPPDDLWERIRRGFAM
                     PDIQSKLVQNNQAYYVARPDYLQRVFDRSRLYLYHIVEEVEKRGMPTELALLPMVESA
                     FNPMAYSRAHASGLWQFIPGTGKRYDLAQNWWYDGRRDIVDSTNAALDYLTKLYQIHG
                     DWQLALASYNWGEGAVGRAIAKNRAAGKPTDYASLKMPKETQNYLPKLQALKNIIANP
                     QPFGIELDPIPNQPYFAEYTGLRDIDVQLAAKLAEMPVDEFVALNPGFSRPLIRAAVT
                     PRIVLPADKIDVFHDNLAKLGEGSLVSWMSYTPKKGETLESIAKKHGMSL"
     gene            complement(1150..1914)
                     /gene="gloB"
                     /locus_tag="VGO02_07275"
     CDS             complement(1150..1914)
                     /gene="gloB"
                     /locus_tag="VGO02_07275"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEV7477521.1"
                     /translation="MLEIVPVKAFKDNYVWTLRNATHAAVVDPGEAQPVLDYLAGEKL
                     ELAAILATHHHADHVGGIAELLQRGRVPVYGPRNEPIPTLTQPVSGGDKVSVPQLGVE
                     FSVLDIPGHTRAHIAYYGAGSLFCGDTLFACGCGRLFEGTAEQMYASLDKLARLPDDT
                     KVYCGHEYTLANIGFAKAVEPANAALRKRETRDRGLREAGRPTLPSTLGEEKATNPFL
                     RCQEPAVVDSVNKYLGSRVSDPVRVFAAIRDWKNKF"
     gene            2072..2683
                     /locus_tag="VGO02_07280"
     CDS             2072..2683
                     /locus_tag="VGO02_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV7477522.1"
                     /translation="MQIGLPEVDFLRQSRIPHRFSLALEAGAAVAADPLQIPLASQSI
                     DLIALPHVLESHDNPHEVLREAERVLMPEGRLVISGFNTASLWRLRQLFISKKRAEPP
                     WDARFIGLLRLRDWLRVLGFELDGGRFGCYAPPFRSRLWLDRFAFMEKAGARWWPITG
                     GIYVVRAVKRVAGMRLITPAWRRERARRRALAPIPQRSHKNHD"
     gene            2676..3113
                     /gene="rnhA"
                     /locus_tag="VGO02_07285"
     CDS             2676..3113
                     /gene="rnhA"
                     /locus_tag="VGO02_07285"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEV7477523.1"
                     /translation="MTEGQVVEIFTDGSCRGNPGPGGWGAILRSGSTEKELFGGEAAT
                     TNNKMELTAVIRALEALKQPSKVSLYTDSQYVQKGISEWIHGWKRNGWKTADKKPVKN
                     KELWVELDELRKKHDIEWHWVKGHAGHPENERADQLANKGIPA"
     gene            3110..3814
                     /gene="dnaQ"
                     /locus_tag="VGO02_07290"
     CDS             3110..3814
                     /gene="dnaQ"
                     /locus_tag="VGO02_07290"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEV7477524.1"
                     /translation="MRLIVLDTETTGLNVKLGDRIVEIGCVEVLSRRISERHFHAYVN
                     PEREIDEGASRVHGLTQEDLRSKPKFAEVARDFLDYVQGAELIIHNADFDVEFLDAEL
                     SRAGLGRLAEHVAKVTDTLAFARELHPGKKNSLDALCERYLIDNSNRTLHGALLDARL
                     LAECYLAMTRGQESLVMELEAPAAVLAAAEALRIDVSSLIVVPALPDEVALHETYLDA
                     MEKDAKGPSLWRRLFG"
     gene            complement(3823..4461)
                     /locus_tag="VGO02_07295"
     CDS             complement(3823..4461)
                     /locus_tag="VGO02_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477525.1"
                     /translation="MVSWLALVFSLVACALAALALFRGRAFAFAPEIFGGDVILPRSS
                     TAGGIKFLLPLQFANAGYAGGVVHWIALRLTVDGDTARSMLLSPVAEVDMQSFLRAKR
                     RLETDNVEPFTGFALDGKRALAKFVLFEPAEQRTAPAPRLRPGRYGFELFYKAGAMRE
                     PLIARSFEHVLEQKSIEQYEADETVYLINYQITLPGVRREVAGLEWLPRGRS"
     gene            4520..6115
                     /locus_tag="VGO02_07300"
     CDS             4520..6115
                     /locus_tag="VGO02_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEV7477526.1"
                     /translation="MSRGSPFEQNLDRNSANYAPLSPLSFIERAASVYPERLAVVYGD
                     QRYTWQETYARSRRLASALAKRGIGRGDTVAVMLPNTPPMYEAHFGVPMCGAVLNALN
                     TRLDPEAIAFMLKHGEAKLLIADPEYLPVIKKSNPSIPVIDAEAGYEGLLQEGDPEYA
                     WLRPQDEWDAIALNYTSGTTGDPKGVVYHHRGAYLNAVCNIVTWGMPHHSVYLWTLPM
                     FHCNGWTFPWTMAANAGTNVCLRKVEASEIFSLIKKHKVTHYCGAPIVHSTLLNAPDE
                     MKAGITHKVSGMVAAAAPPAAMIEGMERIGFDLTHVYGLTEVYGPATVCAKHEEWNQL
                     DIGERTRLNGRQGVRYLMEEGLSVMDPETMQPVPADGETMGEIMFRGNITMKGYLKNP
                     KATQAAFRGGWFHSGDLAVMHPDGYVKIKDRSKDIIISGGENISSLEVEDVLFRHPAV
                     LGAAVVAMPDEKWGETPCAFVEIKPGSKVTEKDIIDFCRQHLAKFKAPRAVVFGELTK
                     TSTGKIQKFQLRERAKSTSAIDT"
     gene            6112..>6566
                     /locus_tag="VGO02_07305"
     CDS             6112..>6566
                     /locus_tag="VGO02_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV7477527.1"
                     /translation="MSAVPQADPPHLLREDRDGICTLTMNRPQQMNLLTSGMLSALQN
                     AFDEISANKKIRVVILAATGKGFSAGHDLKEIKALKELPKIEQLFAQCSRMMQTITML
                     PQPVIARVQGAAAAAGCQLVAQCDLAIASDAARFVTSGVTWGFFCSTPGV"
BASE COUNT         1131 a         2157 c         2221 g         1057 t
ORIGIN      
        1 cgagcgacat gccgtgcttc ttcgcgatcg actccagcgt ctcgcccttc ttcggggtgt
       61 agctcatcca ggaaaccagc gagccttcgc cgagcttggc gaggttgtcg tggaacacgt
      121 cgatcttgtc ggccggcagc acgatgcgcg gggtcaccgc ggcgcggatc agcggccggc
      181 tgaagcccgg gttgagggcg acgaactcgt ccaccggcat ctcggcgagc ttggcggcga
      241 gctgcacgtc gatgtcgcgc agcccggtgt actcggcgaa gtagggctgg ttcgggatcg
      301 ggtcgagctc gatgccgaag ggctgcgggt tggcgatgat gttcttgagc gcctgcagct
      361 tcggcaggta gttctgcgtc tccttcggca tcttcaggct ggcgtagtcg gtgggcttgc
      421 ccgctgcgcg gttcttggcg atcgcccggc cgaccgcgcc ttcgccccag ttataggagg
      481 cgagggcgag ctgccagtcg ccgtgaatct ggtagagctt ggtcaggtag tcgagcgccg
      541 cgttggtgga atcgacgatg tcgcgccggc cgtcgtacca ccagttctgc gccaggtcgt
      601 agcgcttgcc ggtgccgggg atgaattgcc acaggccgga ggcatgcgcc cgcgagtagg
      661 ccatcgggtt gaatgcgctc tcgaccatcg gcagcagcgc gagctcggtc ggcatgccgc
      721 gcttctcgac ctcttcgacg atgtggtaca ggtacagccg gctgcggtcg aacacgcgct
      781 gcaggtagtc gggccgcgcc acgtagtagg cctggttgtt ctgcaccagc ttgctctgga
      841 tgtcgggcat cgcgaagccg cgccggatgc gctcccacag gtcgtccggc ggcacggtgt
      901 gatcgagctt ggccaggccg cgcagcgggt ccttgatcga gcgctgctcg ccgtcgaacg
      961 gcggcagctt cgaggcgtcg acgatgccgg tgccgccggg ggtcggggcg ggggcctgcg
     1021 tcgggaaggg ggtcgggaaa gccgagtcct gcggggccgc cgcggcgggc gacgcaacgt
     1081 ccggcggcgc cgccggcggg gtggcacagg cggcgagcag caccgcgagg atcgctggga
     1141 agatcttgtt caaaacttgt tcttccagtc gcgtattgcg gcgaatactc tcacaggatc
     1201 agaaacgcgt gaaccgaggt acttgttgac cgagtcgacc accgccggct cctggcagcg
     1261 taaaaaaggg ttagtggcct tctcctcgcc gagggtgctg ggcagggtgg ggcggccggc
     1321 ttcgcgcagg ccgcggtcgc gtgtttcacg cttgcgcagc gcggcattgg cgggctcgac
     1381 cgccttggcg aagccgatgt tggcgagcgt gtactcgtgc ccgcagtaga cctttgtgtc
     1441 gtcgggcagc cgcgccagct tgtcgagcga ggcgtacatc tgctccgcgg tgccctcgaa
     1501 caggcgcccg cagccgcagg cgaagagcgt gtcgccacag aaaagcgacc ctgccccata
     1561 ataggcgatg tgggcgcgcg tatggcccgg gatgtcgagc accgaaaatt cgacgccgag
     1621 ctgcggcacg ctcaccttgt cgccgccgga aacaggttgc gtgagcgtcg ggatcggctc
     1681 gttgcgcggt ccgtagaccg gaacgcgacc gcgctgcagg agctcggcga tgccgccgac
     1741 gtggtcggcg tggtggtggg tggcgaggat cgcggcgagc tcgagtttct cgccagcgag
     1801 gtagtcgagc accggctgcg cctcgccggg atcgaccacc gcggcgtgcg tggcgttgcg
     1861 cagcgtccaa acatagttgt ccttgaacgc cttgactgga acaatctcga gcatggaccg
     1921 gcaatccttc ctgcaaaacg gcgcgcagaa gtataacggc gtacgctcat cgctctcatc
     1981 gtggctgacc accccccagg gcgcctacgt cctcgactgg gaatttgcac agtttgacag
     2041 cgctgttgat gacgtgttcg ggtaccgggc catgcagatc ggcctacccg aggtcgattt
     2101 cctgcgacaa agccgcattc cgcaccgctt ttccctcgct ctcgaggccg gcgccgccgt
     2161 cgccgccgac ccgctgcaga tacccctcgc ctcgcagagc atcgacctga tcgcgctgcc
     2221 gcacgtcctc gagtcgcacg acaacccgca cgaggtgctg cgcgaggccg agcgcgtgct
     2281 gatgccagag gggcgtctgg tcatctccgg cttcaacacc gcctcgctgt ggcggctgcg
     2341 gcaattgttc atttccaaga agagggccga gccgccctgg gacgcgcgct tcatcggcct
     2401 gctgcggctg cgcgactggc tgcgcgtgct cggcttcgag ctcgacggcg gccgcttcgg
     2461 ctgctacgcg ccgccgttcc gcagccggct gtggctcgac cgcttcgcct tcatggagaa
     2521 ggccggcgcc aggtggtggc cgatcaccgg gggcatctac gtggtgcgcg cggtgaagcg
     2581 cgtggccggg atgcgcctga tcacaccggc ctggcggcgg gagcgggcgc ggcgccgcgc
     2641 gctcgcgccg atcccgcagc gctcgcacaa gaaccatgac tgaagggcag gtggtggaga
     2701 tcttcaccga cggctcctgc cggggaaatc ccggccccgg tggctggggc gccattcttc
     2761 gttctggcag tactgaaaag gaattgttcg gcggcgaggc cgccacgacc aacaacaaga
     2821 tggagctgac cgcggtgatc cgcgcgctgg aggccctcaa gcagcccagc aaggtgagcc
     2881 tctacaccga ctcgcagtac gtgcagaagg gcatcagcga gtggatccac ggctggaagc
     2941 gcaacggctg gaagaccgcc gacaagaagc cggtcaagaa caaggagctg tgggtcgagc
     3001 tggacgagct caggaaaaag cacgacatcg agtggcactg ggtcaagggg cacgccggcc
     3061 atccggagaa tgagcgcgcg gaccagctgg cgaacaaggg gatacccgcc tgaggctgat
     3121 cgtcctagac accgaaacca ccggattgaa cgtcaagctc ggcgaccgca tcgtcgagat
     3181 cggctgcgtc gaggtgctca gccggcgcat cagcgagcgg cacttccacg cctacgtcaa
     3241 cccggagcgc gagatcgacg agggcgcctc gcgggtgcac ggcctgacgc aggaggacct
     3301 gcgttcgaag ccgaaattcg ccgaggtggc gcgcgatttc ctcgattacg tccagggcgc
     3361 cgagctgatc atccacaacg ccgatttcga cgtcgagttc ctcgatgccg agctctcgcg
     3421 cgccggcctg ggcaggctcg ccgagcacgt cgccaaggtc accgacacgc tggccttcgc
     3481 gcgcgagctg cacccgggga agaagaactc gctcgacgcg ctgtgcgagc gctacctgat
     3541 cgacaactcg aaccgcacgc tgcacggcgc gctgctcgat gcgcgcctgc tcgccgagtg
     3601 ctacctcgcc atgacgcgcg gccaggagag cctggtgatg gagctggagg cgccggcggc
     3661 ggtgctggcc gcggccgagg cgctgcgcat cgacgtctcg agcctgatcg tcgtgcccgc
     3721 gctgcccgac gaggtggcgc tgcacgagac ctacctggac gcgatggaga aggacgccaa
     3781 gggtcctagc ctctggcgcc gcttgttcgg ataaatgcgc ggctagctcc ggccgcgcgg
     3841 cagccactcc aggccggcga cctcgcgccg cacgccgggc agggtgatct ggtagttgat
     3901 gaggtagacc gtctcgtcgg cctcgtactg ctcgatgctc ttttgctcga gcacgtgctc
     3961 gaaggagcgc gcgatcagcg gctcgcgcat ggcgcccgcc ttgtagaaca gctcgaagcc
     4021 gtagcggccg gggcgcagcc gcggcgccgg cgcggtgcgc tgctcggccg gctcgaacag
     4081 cacgaacttg gcgagcgcgc gcttgccatc gagggcgaag ccggtgaacg gctcgacgtt
     4141 gtccgtctcc aggcggcgct tcgcccgcag gaagctctgc atgtccactt cggcgaccgg
     4201 gctgagcagc atcgacctgg cggtgtcgcc gtccacggtc agccgcagcg cgatccagtg
     4261 caccacgccg ccggcgtagc cggcgttggc aaactgcagc ggcagcagga acttgatgcc
     4321 gccggcggtg ctggagcgcg gcaggatgac gtcgccgccg aagatttcgg gtgcgaaggc
     4381 gaaggcgcgg ccgcggaaca gggcgagcgc cgcgagggcg caggccacga gcgagaaaac
     4441 cagggcgagc caggagacca tccgccattg aagggctgcg gcgcgttaaa cgtctacccg
     4501 ccggcgctat actgatcgga tgagccgggg ctcccccttc gagcagaatc tcgacaggaa
     4561 ttcagctaat tacgcgccgc tgagccccct gtccttcatc gagcgcgccg cctccgtcta
     4621 tcccgagcgc ctcgccgtgg tctacggcga ccagcgctac acgtggcagg agacctacgc
     4681 ccgctcacgc cggctcgcct cggcgctggc caagcgcggc atcggccgcg gcgacacggt
     4741 ggccgtcatg ctgccgaaca ccccgcccat gtacgaagcg cacttcggcg tgcccatgtg
     4801 cggcgcggtg ctgaacgcgc tcaacacacg cctcgacccg gaggccatcg ccttcatgct
     4861 gaagcacggc gaggccaagc tcctgattgc agaccccgag tatttgcctg taataaaaaa
     4921 atccaatcca tccattcctg tcatcgacgc cgaggccggc tatgagggtc tgctccagga
     4981 gggcgatccc gagtacgcct ggctgcggcc gcaggacgag tgggacgcca tcgcgctcaa
     5041 ctacacctcc ggcacgaccg gcgacccgaa gggggtcgtc taccaccatc gcggcgcgta
     5101 cctgaacgcc gtctgcaaca tcgtcacctg gggcatgccg caccactcgg tgtacctgtg
     5161 gacgctgccg atgttccact gcaacggctg gacgttcccg tggaccatgg cggcgaacgc
     5221 ggggaccaat gtgtgtcttc gcaaggtcga agcttcagaa attttttctc tgataaagaa
     5281 acacaaagtt acgcactact gcggcgcccc gatcgtccat tccacgctgc tcaacgcgcc
     5341 cgacgaaatg aaggcgggca taacccacaa ggtgagcggc atggtcgcgg cggcggcgcc
     5401 accggcggcg atgatcgagg gcatggagcg catcggcttc gacctcaccc acgtctacgg
     5461 cctgaccgag gtctacggcc cggcgacggt ctgcgccaag cacgaggaat ggaaccagct
     5521 cgacatcggc gagcgcaccc ggctgaacgg ccgccagggc gtgcgctacc tgatggagga
     5581 gggcttgagc gtgatggacc cggaaaccat gcagccggtg ccggccgacg gcgagaccat
     5641 gggcgagatc atgttccgcg gcaacatcac catgaagggc tacctgaaga atcccaaggc
     5701 cacccaggcg gcgttccgcg gcggctggtt ccactcgggc gacctggcgg tgatgcaccc
     5761 cgacggctac gtcaagatca aggaccgctc caaggacatc atcatctccg gcggcgagaa
     5821 tatctcctcg ctcgaggtgg aggatgtgct cttccgtcac ccggccgtgc tcggcgcggc
     5881 ggtggtggcg atgcccgacg aaaagtgggg tgagacgcct tgtgcttttg tcgagatcaa
     5941 gcccggctcc aaagtcaccg agaaggacat catcgatttc tgccggcagc acctggccaa
     6001 gttcaaggcg ccacgcgcgg tggttttcgg cgagctgacg aagacctcca ccggcaagat
     6061 ccagaaattc cagctccgcg agcgggcgaa atccaccagc gcgatcgaca catgagcgcc
     6121 gtccctcaag ccgacccgcc ccacctgctg cgcgaagacc gcgacggcat ctgcaccctc
     6181 accatgaacc gcccgcagca gatgaacctg ctgacgagcg gcatgctgtc cgcgctgcag
     6241 aatgctttcg atgaaatttc tgccaacaag aaaattcgcg tagtgatcct cgccgctacc
     6301 ggcaagggct tcagcgccgg ccacgacctc aaggaaatca aggcgctcaa agagctgccg
     6361 aagatcgagc agctcttcgc ccagtgcagc cgcatgatgc agaccattac catgctcccg
     6421 cagccggtta tcgcccgcgt ccagggcgcc gccgccgcgg ccggctgcca gctcgtcgcg
     6481 caatgcgacc tggcaatcgc ttccgatgcc gccaggttcg tcacctcggg cgtcacctgg
     6541 ggcttcttct gctccacccc cggcgt
//
LOCUS       DASYJA010000332         3572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1866413, whole genome shotgun sequence.
ACCESSION   DASYJA010000332 DASYJA010000000
VERSION     DASYJA010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1866413"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VGO02_07310"
     CDS             <1..701
                     /locus_tag="VGO02_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV7477528.1"
                     /translation="SDSLIVILNDKLEEVLGEDVTQEDAFCAADDVLNNAVAGIAEII
                     NNPGLVNVDFQDVRTVMSEQGMAMMGSALASGIDRARIAAEQAIACPLLEGVNLAGAR
                     GVLVNITASRPSLKLRETKEVMNIIRTFAAEDATIIYGGVYDDSLADDLRVTVVATGL
                     GMASAARQPKPQLVVSQKTGTDNMPVSGSVDYNQLDSVPAVIRRNRASTVEALQQSGV
                     DKYDIPAFLRKQAD"
     gene            786..1997
                     /locus_tag="VGO02_07315"
     CDS             786..1997
                     /locus_tag="VGO02_07315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV7477529.1"
                     /translation="MAKEKRTETEAALPDDGVMFRELFENSATGVALFSIGGRFLRVN
                     PAMCRMLGYSEPELQQKTHLDVIHLDDLEAAAMSRAQIISGKAKSRLAERRYVHKDGS
                     TVWAQVAGAVVRDASGAPLCTVLFANDVSGLKRAVRSSEQRFRRMVEMSSDWYWVQDE
                     QFHFVEVPGLDHQGDDTDIVLGKARWEISGLAPLPEKVWEQHRAKLSRHESFSDFMFL
                     RHNKSGELRYLLVSGEPLFDEQGRFRGYHGIGKDITDRARDQKALEDSEERYRMLFDV
                     QPQPMWVVDAKSLAFLAVNGAAMRLYGYSKEEFLAMTADQIRPEEDVEDLRKAFEDRS
                     RNYRERVWRHRKKNGDLIQVKVISFNLDFDGRAARLGVIHDMTEQLNAEERARQTEER
                     YLALLRERQGT"
     gene            2037..2948
                     /gene="lpxC"
                     /locus_tag="VGO02_07320"
     CDS             2037..2948
                     /gene="lpxC"
                     /locus_tag="VGO02_07320"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEV7477530.1"
                     /translation="MLRQRTLKSLIKASGVGLHTGAKVRMTLRPAPPDTGVVFRRVDL
                     ASPVDVPARAELVGESRLASTLVKGDVKIYTVEHLMSALSGLGIDNLYVDLDAPEVPI
                     MDGSASPFVLLLQQAGIEEQGAPKRFMRVKRRVEVKDGDKWARLDPYEGYKLSFSIDF
                     RHPVIERSTQSVSIDFAETSYLKEIARARTFGFMHEVEALRESGLALGGGLDNAVVLD
                     EYRVLNSDGLRFADEFIRHKLLDAVGDLYLLGRPLLGAFSAHKSGHAINNQLLRAAIA
                     AAAFETVIFERAEEVPSGVARLAAQFG"
     gene            2949..3134
                     /locus_tag="VGO02_07325"
     CDS             2949..3134
                     /locus_tag="VGO02_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477531.1"
                     /translation="MIVVVRILALAVALALGATVLAWLLTGERRWLRIAWLIFRYAVF
                     GLLVVLLLFAGETLWPA"
BASE COUNT          626 a         1175 c         1183 g          588 t
ORIGIN      
        1 gatctgactc gctcatcgtc atcctcaacg acaagctcga ggaggtgctg ggcgaagacg
       61 tgacgcagga agacgccttc tgcgctgccg acgacgtgct caacaacgcg gtcgccggga
      121 tcgccgagat catcaacaat ccgggcctcg tcaacgtcga cttccaggac gtgcgcacgg
      181 tcatgtccga gcagggcatg gcgatgatgg gctcggcact ggcgagcggc atcgaccgcg
      241 cgcgcatcgc cgccgagcag gcgatcgcct gcccgctgct cgagggcgtg aacctcgccg
      301 gcgcgcgcgg cgtgctggtg aacatcaccg cctcgcgccc gagcctgaag ctgcgcgaga
      361 cgaaggaggt gatgaacatc atccgcactt tcgcggcgga ggatgccacc atcatctacg
      421 gcggcgtgta cgacgattcg ctggccgacg acctgcgcgt cacggtagtg gcgacgggcc
      481 tgggcatggc gagcgcagcc cgccagccca agccgcaact cgtcgtctcg cagaagaccg
      541 gcaccgacaa catgccggtg tccgggtcgg tggactacaa ccagctcgac tcggtgcccg
      601 ccgtcatccg gcgcaaccgg gcctcgacgg tggaggcgct gcagcagagc ggggtcgaca
      661 agtacgacat cccggcgttc ctgcgcaagc aggccgacta gcgccgctgg agtgtaaaaa
      721 aagcgtcggg cgctcttcct tgccgggtgc ccgacgctcg cttcctgtgt catcataaga
      781 ggctcatggc aaaggaaaaa cgcaccgaaa ccgaagctgc attgcccgac gacggggtga
      841 tgttccgcga gctgttcgag aattccgcaa ccggggtggc gctgttctcg atcggcggcc
      901 gtttcctgcg cgtcaacccg gcgatgtgcc ggatgctcgg ctacagcgag ccggagctgc
      961 agcagaagac ccacctcgac gtgatccacc tcgacgatct cgaggccgcg gccatgagcc
     1021 gggcgcagat catctccggc aaggccaagt cgcgcctcgc cgaaaggcgt tacgtccaca
     1081 aggacggctc cacggtatgg gcgcaggtgg ccggcgccgt ggtgcgcgac gcctccggtg
     1141 cgccgctgtg cacggtgctc ttcgccaacg acgtcagcgg cctcaagcgc gcggtgcgct
     1201 ctagcgagca gcgctttcgc cgcatggtcg agatgagctc ggactggtac tgggtgcagg
     1261 acgagcagtt ccatttcgtc gaggtgcccg gcctcgacca ccagggcgac gacaccgaca
     1321 tcgtgctcgg caaggcgcgc tgggagatct ccggcctcgc gccgctgccc gagaaggtgt
     1381 gggagcagca ccgcgccaag ctgtcgcgcc acgaatcgtt ctcggacttc atgttcctgc
     1441 gccacaacaa gtcaggcgag ctgaggtacc tgttggtctc gggcgagccg ctgttcgacg
     1501 agcaggggcg cttccgcggc taccacggca tcggcaagga catcaccgac cgggcgcgcg
     1561 accagaaggc gctcgaggac agcgaagagc gctaccgcat gctgttcgac gtgcagccgc
     1621 agcccatgtg ggtggtcgac gcgaagagcc tcgcgttcct cgccgtgaac ggcgccgcga
     1681 tgcgcctcta cggctactcg aaggaagagt tcctcgccat gacggccgac cagatccgcc
     1741 cggaagagga cgtggaggat ctgcgcaagg ccttcgagga ccgcagccgc aactatcgcg
     1801 agcgggtctg gcggcaccgc aagaagaacg gcgatctgat ccaggtgaag gtgatctcct
     1861 tcaacctcga cttcgacggc cgcgccgcgc gcctgggcgt gatccacgac atgacggagc
     1921 agctaaacgc cgaagagcgc gcgcgacaga ccgaagagcg ctatctcgca ctgctgcgag
     1981 agaggcaagg cacttaaggc aggtactgcg catagcagct agaatccgcc ccccaaatgc
     2041 tccgccaacg cactctcaaa tcgctcatca aggcctccgg cgtcggtctg cataccggcg
     2101 cgaaggtgcg catgaccttg cgccccgcgc cgcccgacac gggcgtggtg ttccgccgcg
     2161 tcgacctcgc ctcgccggtg gacgtgccgg cgcgcgccga gctggtcggc gagtcgcgcc
     2221 tcgcgtccac cctggtcaag ggagacgtca agatctatac ggtcgagcac ctcatgtcgg
     2281 cgctcagtgg cctgggcatc gacaacctct atgtcgacct ggacgcgccc gaagtgccga
     2341 tcatggacgg cagcgcctcg cccttcgtcc tgctgctgca gcaggcgggc atcgaggagc
     2401 agggtgcgcc caagcgcttc atgcgggtca agcgccgcgt cgaagtgaag gacggcgaca
     2461 agtgggcgcg cctcgatcct tacgagggct acaagctctc tttctcgatc gatttccgcc
     2521 atccggtgat cgagcgctcg acgcagtccg tgtcgatcga tttcgccgag acctcgtatc
     2581 tcaaggaaat agcgcgcgcg cgcaccttcg gcttcatgca cgaggtcgag gcgctcaggg
     2641 aaagcggcct cgcgctcggc ggcgggctcg ataacgcagt ggtgctggac gagtaccggg
     2701 tgctgaattc cgacggcctg cgcttcgccg acgaattcat ccgccacaag ctgctcgacg
     2761 cggtcggcga cctgtacctg ctcggccggc cgctgctcgg cgccttcagc gcccacaagt
     2821 cgggccatgc catcaacaac cagctgctgc gcgcggcgat cgccgccgcg gcattcgaga
     2881 ccgtgatctt cgagcgcgcc gaggaggtgc cgtccggcgt cgcgcgcctc gcggcccagt
     2941 tcggctagtt gatcgtcgtc gtccggatcc tcgcgcttgc cgtggcgctc gcgctcggcg
     3001 ccaccgtcct tgcctggctc ctgaccggtg agcgccgctg gctgcgcatc gcctggctga
     3061 ttttcaggta cgccgttttc ggcctgctgg tggtgctgct ccttttcgcg ggggagactc
     3121 tttggcctgc ctagggtcgt ggcggcgcag aaggctccct agcgcctcgc gcgccgggga
     3181 gttctgcatg ctgtcgtaaa gcgcttgtaa ggaatccagc gtaggtgtgg ataaatacgc
     3241 agttttttgc ggcgcagcaa gcgtatttcc gggcggattt ggttgcaccc tcacggaaac
     3301 tgaactaacc tgacatcgct gttcgatcaa gaagcggctc agggaagggg ccagcagttt
     3361 cagcttggca gccgcggagg aattggcggc cagaagcacc agttctccct ccctgatgtt
     3421 ggccacgcgg acctggcaag ccaggtccgg cggcagaaag ccgtcgacga acccggcgag
     3481 cgcgccgatc tcgcgcgttt tcgctactag gggctgcaat tcgccgtcgg ccgaaagaat
     3541 ccggccgagt ttgggaagtc gcacggggac cg
//
LOCUS       DASYJA010000333         7394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2682639, whole genome shotgun sequence.
ACCESSION   DASYJA010000333 DASYJA010000000
VERSION     DASYJA010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7394
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2682639"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VGO02_07330"
     CDS             complement(<1..618)
                     /locus_tag="VGO02_07330"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF012496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEV7477532.1"
                     /translation="MDKALLSKTAAAMVAKGKGVLAADESSGTCEKRFKSVGVECTEE
                     SRRTYRNLLFSATGIEQYLSGVILFDETARQKAPDGSLIPQYLAKKGILPGIKVDKGV
                     ANIPFTREEKVTEGLDGLQKRMKEYFDLGCRFAKWRAVITIGEGIPTHSCLYANAHVL
                     ARYAAACQGASIVPMIEPEVLLDGDHTAERSEEVHEATLRATYAAM"
     gene            719..2542
                     /gene="typA"
                     /locus_tag="VGO02_07335"
     CDS             719..2542
                     /gene="typA"
                     /locus_tag="VGO02_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEV7477533.1"
                     /translation="MKPDQRNLAIIAHVDHGKTTMVDKLLRQSGTFAAHEHLEERVMD
                     SNDLERERGITILAKNCAVRYKGTHINIVDTPGHADFGGEVERVLSMVDGVLLLVDAV
                     EGPMPQTRFVTRKALALGLKPIVVVNKVDRPGARADWAVNQTFDLFDKLGATEAQLDF
                     PVIYASALEGWATKDLKHPEKDLAALFQAILEYIPVREDEDPEGPLQLQICSLDYSSY
                     VGKIGIGRIRRGRLKAAQEVLVLNGPGAEPIKAKVNQILMFEGLERVQVDEAQAGDIV
                     LVNGVDEVGIGVTLTDLEHPEALPLLKVDEPTLTMNFLVNASPLAGREGKFVTSRQIR
                     ERLERETKSNVAMRVEYPEDTDTFIVHGRGELHLTILLENMRREGYEIAVSRPRVVYK
                     EIDGVRCEPFEFLSVDVEDQNQGAVMEELGSRRGELKDMQSDSKGRVRLDYRIPARGL
                     IGFQSEFMTLTRGTGLMSHVFDDYAPAKTEEMAERRNGVLVSQDDGVAVAYALWKLQE
                     RGRMFVSPGDPVYEGMIIGIHSRDNDLVVNPIKTKQLTNIRASGKDEAIDLTPPIQLT
                     LEYAVEFIGDDELAEITPKSIRLRKRHLQEHDRRRAERAAA"
     gene            2562..3008
                     /locus_tag="VGO02_07340"
     CDS             2562..3008
                     /locus_tag="VGO02_07340"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEV7477534.1"
                     /translation="MRGVAAIFLGFMVAGCASNPVAPVTDPGSADRAAGSALKMVGKP
                     YRYGGASPAGFDCSGLVQYSYKQAGVSLPRATDAQLRISQPLRGQHLRRGDLLFFDQE
                     GKKKSHVGIYLGDGRFVHAPSSGKIVRTDRLDSAFWKKHLSEVRRI"
     gene            complement(3005..4264)
                     /locus_tag="VGO02_07345"
     CDS             complement(3005..4264)
                     /locus_tag="VGO02_07345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7477535.1"
                     /translation="MEEDGRRDRGESRIASAKHDVIVVGAGAAGLAAADALARAGRSV
                     LVLEARSRIGGRCWTRRMPGLPVPVELGAEFIHGRAQATLGLLARAGVPGVDSTRTQR
                     FLEHGRLREANAFVEAHKAVQHAALLKKRDLSFSDFLAAKKLNPKTTKLATMMVQGFD
                     AADPTRVSAREIAEEWGSGALGSSQMRPQGGYGPLLETFLTGKFQVALGRPVREVRWQ
                     GGAVEIDGIRARHAIVTLPLGVLQSGSVRFFPEIEKQAALQKLASGPVVRVALRFREA
                     FWEELCPGTAFFHSPEAPFPTFWTPLPMHAPLLTAWAGGPKAERLSGLPEKQLVRHAL
                     ASVRSVLGRVPKVQGVLVQDWQADPYSLGAYSYVLVGGQGARQQLRRPLLKTLFFAGE
                     ATDTDEAGTVAGALRSGARAAREVLAT"
     gene            complement(4222..5184)
                     /locus_tag="VGO02_07350"
     CDS             complement(4222..5184)
                     /locus_tag="VGO02_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7477536.1"
                     /translation="MRFLALLLLPIWATCLSFGAAAQTYPAKPVRIIVPFAPGGGSDF
                     IARFTAQRLSGSFGSQVIVENKPGAGGLIGIEQGVKADPDGYTFTLIASSYTVNPALY
                     SLKYDPVNDITPVVQISQGPMLVVVNPKVPAKSATELIALAKAKPGTLNFASAGQGSI
                     THMACELFAYMAGIKMNHIPYKGTGPALTDTIGGQTDLFCTSTATALPHVKAGRLRAL
                     AVTTANRLPAEPNVPTLAESGVKGYDVPLWHGLIAPKGMPKVAVDKMNAEVNKLLKSK
                     DTAEQLQTDGVAPKGGTPEAFRDQIRKEVELWKKTVAATGVKAE"
     gene            5229..6149
                     /locus_tag="VGO02_07355"
     CDS             5229..6149
                     /locus_tag="VGO02_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7477537.1"
                     /translation="MIKIENLVKAFGAKRAVDDISFSVERGEVLGFLGPNGAGKSTTM
                     RMVTGFMPPTSGRISIGGHDVVDSPLEAKRLIGYLPENAAAYPDMTVHGFLSFVAELR
                     GLRGDARRKAVQRVVDLCFLESVLPQSIDTLSKGYRHRTCLAQALIHDPEVLIMDEPT
                     DGLDPNQKHEVRNLIRNLGKSKAIVFSTHILEEVDAACTRAIIIDRGRIVANGTPEEL
                     RSMSALAGAVTISAHGASAEKLAELGRVEDLDGAFRVYPHDKSKAKELARRIVDLVNR
                     EGWKVEGMFSEAGQLDEVFRRITLPDTVKR"
     gene            6146..6880
                     /locus_tag="VGO02_07360"
     CDS             6146..6880
                     /locus_tag="VGO02_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7477538.1"
                     /translation="MRAVWIIAKRELASYFTSPVAYVFLVIFLLLTGFFTFTAGNFFE
                     RGEASLAAFFGWHPWLYLVLVPAVGMRLWAEERRSGTLELLLTMPITPWQAIVAKFLA
                     SWVFLAVALLLTFPAVITVNVLGEPDNGIIFAGYLGSFLLAGAYLAISCMTSAMTRNQ
                     VVAFILSVVICLFLLLAGFNPVTDLLVRWASPAVVDTVAAFSVVTHFDGFQRGVIDTR
                     DLAFFLSVIGFTLFATGVIIRGHRAG"
     gene            6870..>7394
                     /locus_tag="VGO02_07365"
     CDS             6870..>7394
                     /locus_tag="VGO02_07365"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HEV7477539.1"
                     /translation="MPASFLRKNEQLVYSAVGLVALFLLLVALNFLLTLAPVRIDLTQ
                     GKLYTLSPGTKKILRNLSSPVKVKLYVSQGESVPVPLRGFAQRVEDLVREFKQAGGSN
                     LIVERYNPRPDSEEEDAAQLDGIEPQQLVSGEQFYLGVAVSQLERKQAIPAISPQRER
                     LIEYDLVRAVARVGS"
BASE COUNT         1219 a         2493 c         2451 g         1231 t
ORIGIN      
        1 catcgccgcg taagtagcgc gcagcgtcgc ttcatgcact tcttccgagc gctccgcggt
       61 gtggtcgccg tcgagcagga cctcgggctc gatcatcggc acgatggaag ccccctggca
      121 ggccgcggca taacgcgcga gcacgtgcgc attggcatag agacagctgt gggtcgggat
      181 gccttcgccg atggtgatca ccgcgcgcca cttggcgaag cggcagccca ggtcgaagta
      241 ctccttcatc cgcttctgca ggccatcgag accctcggtg accttctcct cgcgggtgaa
      301 ggggatgttg gcgaccccct tgtccacctt gatgcccggc aggatgcctt tcttcgccag
      361 gtattgcggg atcaggctgc cgtcgggcgc tttctgccgc gcggtctcgt cgaacaggat
      421 cacgcccgac aggtactgct cgatgcccgt ggcgctgaac agcaggttgc ggtaggtgcg
      481 gcggctttct tcggtgcatt cgacgccgac cgacttgaag cgcttctcgc aagtgccgct
      541 cgattcatcg gcggcgagta cgcccttgcc cttcgcgacc atcgcggccg cggttttcga
      601 aaggagtgct ttgtccatgg attcgtgaaa attggtgctg tgatgagggt ttacgggaag
      661 gaggcaggga gattaagccg agattttaac caattactcg cgtacaatgc gcgcccctat
      721 gaagcccgat cagcgcaatc tcgcaatcat cgcccacgtc gaccacggca agaccaccat
      781 ggtcgacaag ctgctgcgcc agtcgggcac gttcgctgcc cacgagcacc tcgaagagcg
      841 cgtgatggac tccaacgacc tggagcgcga gcgcggcatc acgatcctcg ccaagaactg
      901 cgcggtccgc tacaagggca cccacatcaa catcgtcgat accccgggcc acgccgactt
      961 cggcggcgag gtcgagcggg tgctctccat ggtcgacggc gtgctgctgc tggtcgacgc
     1021 ggtcgaggga ccgatgccgc agacgcgctt cgtcacgcgc aaggcgctgg cgctcggcct
     1081 caagccgatc gtggtggtga acaaggtcga tcgccccggc gcgcgcgccg actgggcggt
     1141 caaccagacc ttcgatctgt tcgacaagct cggcgccacc gaggcgcagc tcgatttccc
     1201 ggtgatctat gcttccgccc tcgagggctg ggccaccaag gatctgaagc acccggaaaa
     1261 ggacctcgcg gcgctgttcc aggccatcct cgagtacatc ccggtgcgcg aggacgagga
     1321 cccggaaggc ccgctgcagc tgcagatctg ctcgctcgac tactcgagct acgtcggcaa
     1381 gatcggcatc ggccgcatcc gccgcgggcg gctgaaggcg gcgcaggagg tgctggtgct
     1441 caacggcccc ggcgccgagc cgatcaaggc caaggtcaac cagatcctca tgttcgaggg
     1501 gctggagcgc gtgcaggtcg acgaggcgca ggccggcgac atcgtcctgg tgaacggcgt
     1561 cgacgaagtg ggcatcggcg tcacgctcac cgacctcgag catcccgagg cgctgccgct
     1621 gctcaaggtc gacgagccga cgctcaccat gaacttcctg gtcaacgctt cgccgctcgc
     1681 cgggcgcgag gggaaattcg tcaccagccg ccagatccgc gagcggctgg agcgcgagac
     1741 caagagcaac gtcgcgatgc gcgtcgagta ccccgaggac accgatacct tcatcgtgca
     1801 cgggcgcggc gagctgcacc tgaccatcct gctcgagaac atgcgccgcg aaggctacga
     1861 gatcgcggtg tcgcggccgc gcgtggtcta caaggaaatc gacggcgtgc gctgcgagcc
     1921 gttcgaattt ctgagcgtcg acgtcgaaga tcagaaccag ggcgcggtga tggaagagct
     1981 gggaagtcgc cggggtgagc tgaaagacat gcagtcagac agtaaagggc gcgtgcgcct
     2041 ggactaccgc atcccggcgc gcgggctgat cggcttccaa agcgagttca tgacgctcac
     2101 ccgcggcacc ggcctgatga gccacgtctt cgacgactac gcgcccgcca agaccgagga
     2161 gatggccgag cggcgcaacg gcgtgctggt gtcgcaggac gacggcgtcg cggtggccta
     2221 cgcgctgtgg aagctgcagg agcgcgggcg catgttcgtc agccccggcg acccggtgta
     2281 cgagggcatg atcatcggca tccacagccg cgacaacgat ctggtggtaa atccgatcaa
     2341 gaccaagcag ctcaccaaca tccgcgcctc cggcaaggac gaggcgatcg acctgacgcc
     2401 gccgatccag ctcacgctgg agtacgcggt ggaattcatc ggcgacgacg agctcgccga
     2461 gatcacgccc aagtcgatcc gcctgcgcaa gcgccacctg caggagcacg accggcgccg
     2521 cgcggagcgt gcagccgcct aagcaggtat cctctctccg catgagaggg gttgccgcga
     2581 tctttctcgg tttcatggtg gcgggatgcg ccagcaaccc cgtcgcgccc gtgaccgacc
     2641 ccggctcggc cgatcgcgcc gccggcagcg cactcaagat ggtgggtaag ccctaccgct
     2701 acggcggcgc cagtcccgcc ggcttcgatt gcagcggcct cgtccagtac agctacaagc
     2761 aggcgggtgt gtcgctaccg cgcgccaccg acgcgcagct gcgcatctcg cagccgctgc
     2821 gcggccagca cctgcgacgc ggcgatctgc tgttcttcga tcaggaaggc aagaagaagt
     2881 cgcacgtcgg catctacctg ggcgacggca ggttcgtgca cgcgccttcg tcgggcaaga
     2941 tcgtgcgcac cgaccggctc gattcggcct tctggaagaa gcacctctcc gaggtgcggc
     3001 gcatctaggt cgcgaggact tcgcgcgccg cgcgcgcgcc gctgcgcaag gcgccggcaa
     3061 ccgttcccgc ttcgtccgta tcggtcgctt caccggcgaa gaagagcgtc ttcagcagcg
     3121 gcctgcgcag ctgctggcgc gcgccctgcc ccccgaccag cacgtagctg taggcgccca
     3181 gcgaatacgg gtcggcctgc cagtcctgca ccagcacgcc ctgcactttg ggaacccgcc
     3241 ccagcacgga ccggacgctg gcgagcgcgt gtcgaacgag ttgtttttcg ggcaagccgg
     3301 aaaggcgctc ggccttgggg ccgccggccc acgcggtgag cagcggtgca tgcatcggca
     3361 gcggcgtcca gaaagtcgga aacggcgcct cgggcgaatg gaagaatgcg gtacccgggc
     3421 acagctcctc ccagaatgct tcgcgaaagc gcagcgcgac cctgacgacg ggacccgagg
     3481 cgagcttttg cagcgcagcc tgcttttcaa tttccggaaa gaacctgacc gaccccgact
     3541 gcagcacgcc tagcggcagc gtgacgatcg cgtgcctggc gcgaatgccg tcgatctcga
     3601 ccgcgccgcc ttgccagcgg acttcacgca ccggcctgcc cagcgcgacc tggaacttgc
     3661 ccgtgaggaa cgtctcgaga agcggtccgt agccgccctg cggccgcatc tgcgacgagc
     3721 cgagcgcgcc gctcccccat tcctcggcga tctcgcgcgc gctgacgcgc gtcgggtcgg
     3781 cggcgtcgaa gccctgcacc atcatggtcg cgagcttcgt ggtttttgga ttgagtttct
     3841 tcgcagcgag gaagtcggag aaggacaaat cccttttctt tagcaatgcg gcgtgctgca
     3901 ccgccttgtg cgcctcgacg aaggcattcg cctcgcgcag cctgccgtgc tccaggaagc
     3961 gctgcgtgcg cgtggaatcg acgcccggaa cgccggcgcg cgcgagcagt ccgagggtgg
     4021 cttgcgcccg cccatggatg aactcggcgc cgagctcgac cgggaccggc aggccgggca
     4081 tgcggcgggt ccagcagcgc ccgccgatgc ggctgcgtgc ctcgaggacc aggaccgagc
     4141 ggccggcgcg cgccagcgcg tcggcggccg ccaggccggc cgcgccggcg ccgacgacga
     4201 ttacatcgtg cttggcggag gctattctgc tttcaccccg gtcgcggcga ccgtcttctt
     4261 ccacagctcg acttccttgc ggatctggtc gcggaacgcc tcgggcgtgc cgcccttggg
     4321 cgctacgccg tcggtctgca gctgctcggc ggtgtccttg gatttgagca gcttgttgac
     4381 ctcggcgttc atcttgtcga ccgcaacctt gggcatgccc ttcggcgcga tcagcccgtg
     4441 ccagagcggc acgtcgtacc ccttgacccc cgactcggcg agcgtcggaa cattcggctc
     4501 ggccggcagt ctattggcag tcgtcaccgc gagggcccgc aggcgccccg ccttgacgtg
     4561 cggcagcgca gtcgcggtgc tggtgcaaaa cagatcggtc tgcccgccga tggtatcggt
     4621 gagcgcggga ccggtcccct tgtaaggaat atggttcatc ttgattcccg ccatataggc
     4681 gaacagctcg cacgccatgt gcgtgatgct gccctgcccg gcggaggcga agttgagtgt
     4741 gccgggtttc gccttggcga gcgcgatcag ctcggttgcg ctcttggcgg gcactttggg
     4801 gttcaccacc accagcatcg gcccctgcga gatctgcacc accggcgtga tgtcgttgac
     4861 cgggtcgtac ttgagggaat agagcgcggg gttcacggtg tagctcgagg cgatgagcgt
     4921 gaacgtgtag ccgtcggggt cggccttgac gccctgctcg atgccgatca ggccgccggc
     4981 gccgggcttg ttctcgacga tcacctgcga gccgaacgag cccgacaggc gctgcgcggt
     5041 gaagcgcgcg atgaagtcgg agccgccgcc gggcgcgaac ggcacgatga tgcgcaccgg
     5101 cttcgccggg taggtctggg cggcggcgcc gaaggaaagg caggttgccc aaatgggaag
     5161 aagcagcagg gcgaggaaac gcatgatttt caggctccaa agttgagcga taattgcgcc
     5221 cctccaacat gatcaagatc gagaacctcg tcaaggcttt cggcgcgaaa cgcgccgtgg
     5281 acgacatttc cttcagcgtc gagcgcggcg aggtgctcgg ctttctcggc ccgaacggcg
     5341 ccggcaagtc gaccaccatg cgcatggtga ccggcttcat gccgccaacc tcgggcagga
     5401 tcagcatcgg cggccacgac gtcgtcgatt cgccgctgga agcgaagcgc ctgatcggct
     5461 acctgcccga gaacgcggcc gcctaccccg acatgaccgt gcacggcttc ctcagcttcg
     5521 tcgccgagct gcgcggcctg cgcggcgacg cgcgccgcaa ggcggtgcaa cgcgtggtcg
     5581 acctgtgctt cctggaatcg gtgctgccgc agtcgatcga cacgctctcc aagggctacc
     5641 ggcaccgcac ctgcctggcg caggcgctga tccacgatcc cgaggtgctg atcatggacg
     5701 agcccaccga cggcctcgac ccgaaccaga agcacgaagt gcgcaacctc atccgcaacc
     5761 tcggcaagtc gaaggcgatc gtcttctcga cccacatcct cgaggaagtc gacgccgcct
     5821 gcacgcgcgc catcatcatc gaccgcggcc gcatcgtggc gaacggcacc cccgaagagc
     5881 tgcgcagcat gtcggcgctg gcgggcgcgg taaccatctc ggcgcacggc gcgagcgcgg
     5941 agaagctcgc cgagctcggc cgcgtcgagg atctggacgg cgcgttccgt gtctacccgc
     6001 acgacaagtc gaaggcaaag gagctcgcgc gcaggatcgt cgacctggtc aaccgcgagg
     6061 gctggaaggt cgagggcatg ttcagcgagg ccggccagct cgacgaggtg ttccgccgca
     6121 tcaccctgcc cgatacggtg aagcgatgag ggcggtctgg atcatcgcca agcgcgagct
     6181 tgccagctac ttcacctcgc cggtggcgta cgtcttcctg gtgatcttcc tgctgctcac
     6241 cggcttcttc accttcaccg cgggcaattt cttcgagcgc ggcgaagcca gcctggccgc
     6301 gttcttcggc tggcatccct ggctctacct ggtgctggtg cccgccgtcg gcatgcgcct
     6361 gtgggccgag gagcgccgct ccggcacgct cgagctgctg ctcaccatgc cgatcacgcc
     6421 ctggcaggcg atcgtcgcga aattcctcgc ctcgtgggtg ttcctcgcgg tggcgctgtt
     6481 gctcaccttt cccgcggtga tcacggtcaa cgtgctcggc gaaccggaca acggcatcat
     6541 cttcgccggc tacctcggca gcttcctgct ggccggcgcc tacctcgcga tctcgtgcat
     6601 gacctcggcg atgacgcgca accaggtggt cgcgttcatc ctgtcggtgg taatttgtct
     6661 gttcttgctg ctcgccgggt tcaacccggt gaccgacctg ctggtgcgct gggccagccc
     6721 ggcggtggtc gacacggtgg ccgcgttctc ggtggtcacg cacttcgacg gcttccagcg
     6781 cggcgtcatc gacacgcgcg acctcgcctt cttcctctcg gtgatcggct tcaccctgtt
     6841 cgccaccggc gtcatcatcc gggggcaccg tgccggctag cttcctcagg aagaacgagc
     6901 agctggtcta ctcggcggtc ggcctggtcg cgctgttcct gctgctggtg gcgctcaact
     6961 tcctgctcac gctcgcgccg gtgcgcatcg acctgacgca gggcaagctc tacaccctgt
     7021 cgccgggaac caagaagatc ctgcgcaacc tgtcctcgcc ggtgaaggtg aagctctacg
     7081 tatcgcaggg cgagtcggtg ccggtgccgc tgcgcggctt cgcgcagcgc gtcgaggacc
     7141 tggtgcgcga gttcaagcag gcgggcggct ccaatctcat cgtcgagcgc tacaacccgc
     7201 ggcccgactc ggaggaggag gacgcggcgc agctcgacgg catcgagccg cagcagctgg
     7261 tgagcggcga gcagttctac ctcggcgtgg cggtgagcca gctcgagcgc aagcaggcga
     7321 tccccgccat ctcgccgcag cgcgagcggc tgatcgagta cgacctggtg cgcgccgtcg
     7381 cgcgcgtcgg ctcg
//
LOCUS       DASYJA010000334         2556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2046903, whole genome shotgun sequence.
ACCESSION   DASYJA010000334 DASYJA010000000
VERSION     DASYJA010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2046903"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VGO02_07370"
     CDS             complement(<1..573)
                     /locus_tag="VGO02_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEV7477540.1"
                     /translation="MNAPLIGARIARKEDYRFLTGAGQYTDDVTLQNQSYAAFVRSPH
                     AHARIRSISIAKAKAAPGVVGVFVGKDLADAKVGGLPCGWLITDVNGQPMKEPPHPCL
                     AQGKVRHVGDQVAVVIAETLAQARDAAELVDVDYEVLPAVVDVSSATKKPPIHDGVPN
                     NTCYVWALGDKAACDTAFAKAAHVTKLEFVN"
     gene            complement(570..1049)
                     /locus_tag="VGO02_07375"
     CDS             complement(570..1049)
                     /locus_tag="VGO02_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEV7477541.1"
                     /translation="MTIKVSLKVNGKAVSAEVEERTLLVTLIRENLRLTGTHVGCDTA
                     QCGACTVHMNGKAIKSCSIVAMQAEGAEVLTIEGVAKADGTLHPMQQAFKDNHGLQCG
                     FCTPGMVMNALDFAKGHPSPTEQEVRESLEGNLCRCTGYHNIVKSIVAGAKAMGGKL"
     gene            1127..1810
                     /locus_tag="VGO02_07380"
     CDS             1127..1810
                     /locus_tag="VGO02_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7477542.1"
                     /translation="MRLLIVEDDAPLASGLRRILEAEGHAVDVTGRGEEAVSAAAHER
                     FDLVILDIGLPQMDGFEVLRRFRAAQTDKGAPMQVLVLTARDAIDDRVRGLDLGADDY
                     MVKPFAMPELTARVRALLRRSQSHGGPRIVHGPLALDTVARRAYLRNEPLELAAREWA
                     VLEVLLAKVEKIVSKEAIIQAVAGWGDDLSPNAIEVYVSRLRSKLEPAGIKIRTVRGF
                     GYMLEEFKG"
     gene            1835..>2556
                     /locus_tag="VGO02_07385"
     CDS             1835..>2556
                     /locus_tag="VGO02_07385"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HEV7477543.1"
                     /translation="MARSLRAHLLRLLLPPIAALLAVGAVVAYYPSMEPATAAYNQAL
                     VDVGIALGSYVRNADTGYSFDLPPAVEQVLRTDRYDTIYYRVITPEGRNIAGEPDLPM
                     PPDATNGSYEAQFKGQRVHVVSVQSPCGSSACNILVAETTVKRTRLTREILFSSLMPQ
                     MVLAVATLVIVWFGVKRGLGPLARLSEEIKGRSPRDLHPIDAAAAPEETRPLLAALNG
                     LLEQVSEASRNQQRFLANAAHQL"
BASE COUNT          398 a          862 c          843 g          453 t
ORIGIN      
        1 gttgacgaac tcgagcttgg tgacgtgcgc ggctttcgcg aacgccgtgt cgcaggcggc
       61 tttgtcgccg agcgcccaca cgtagcaggt gttgttcggc acgccgtcgt gaatgggagg
      121 ctttttcgtt gccgaagaaa catccacaac cgccggcagc acttcgtagt ccacgtcgac
      181 cagctcggcg gcgtccctcg cctgcgcgag cgtctcggcg atcaccaccg cgacctggtc
      241 gccgacgtgg cgcaccttgc cctgcgcgag gcagggatgc ggcggctcct tcatgggctg
      301 gccgttgacg tcggtgatca gccagccgca ggggaggcct cccactttcg cgtcagcaag
      361 atcttttcca acaaaaactc caaccactcc cggcgcggcc tttgcctttg caatcgagat
      421 gctcctgatc cgggcatgcg cgtggggcga acgaacgaat gccgcatagg actggttctg
      481 cagcgtcacg tcatccgtgt actgtccggc gcctgtcagg aacctgtagt cttccttgcg
      541 cgcgatgcgc gcgccgatga gcggggcatt cacaatttgc ctcccatcgc cttggcgccg
      601 gcgacgatgc tcttcacgat gttgtggtag ccggtgcagc ggcacaggtt gccttccagg
      661 ctttcgcgca cctcctgctc ggtcgggctg gggtgtccct tcgcgaagtc gagcgcgttc
      721 atcaccatcc cgggcgtgca gaagccgcac tgcaggccgt ggttgtcctt gaacgcctgc
      781 tgcatcgggt gcagggtgcc gtcggctttc gccacgccct cgatggtgag gacctcggcg
      841 ccctcggcct gcatggcgac gatggagcaa cttttgatcg ctttgccgtt catatgtacg
      901 gtgcaggcgc cgcattgggc ggtgtcgcag ccgacgtggg taccggtcag gcgcaggttc
      961 tcccggatca gggtgacgag cagagtgcgc tcctcgacct cggcggaaac ggccttgcca
     1021 ttgactttca acgagacctt gatggtcatg cggatgcctc ctcggggaaa attatcctag
     1081 cgcgggcgta tcatttacgc tactccaata aagcgagtcc accactatgc ggctcctcat
     1141 cgttgaagac gacgcgcccc tggcctccgg cctgcggcgc attctggaag ccgaaggcca
     1201 cgcggtcgac gtcaccggcc gcggcgagga ggcggtaagc gccgccgcgc acgagcgctt
     1261 cgacctcgtg atcctcgaca tcggcctgcc gcagatggac ggcttcgagg tgctgcggcg
     1321 cttccgtgcc gcacagaccg ataagggcgc gccgatgcag gtgctggtgc tgaccgcgcg
     1381 tgacgccatc gacgaccgcg tgcgcggcct ggacctcggc gccgacgact atatggtgaa
     1441 gccctttgcc atgcccgagc tcacggcgcg ggtacgggca ttgctgcgcc gctcgcagtc
     1501 gcacggcggg ccgcgcatcg tgcacgggcc gctcgccctc gataccgtgg caagaagggc
     1561 ctatttaaga aatgaaccct tggaacttgc ggcgcgcgaa tgggcggtgc tcgaagtgct
     1621 gctcgccaag gtcgagaaga tcgtctcgaa ggaggcgatc atccaggccg tcgcgggctg
     1681 gggcgacgac ctttcgccca acgccatcga ggtctacgtc tcgcggctgc gctccaagct
     1741 cgagccggcc ggcatcaaga tccgtaccgt gcgcggcttc ggctacatgc tcgaggagtt
     1801 caagggttag ggcctatcgg tcagggccag gctgatggcg cgcagcctgc gcgcgcacct
     1861 cctgcgcctg ctgctgccgc cgatcgccgc gcttctcgcg gtgggcgcgg tggtggcgta
     1921 ctacccgtcg atggagccgg cgactgccgc ctacaaccag gcgctggtcg acgtcggcat
     1981 cgccctgggc agctacgtgc gcaacgcgga cacgggctac tccttcgacc tgccgccggc
     2041 ggtcgagcag gtgctgcgca ccgaccgcta cgacaccatc tactaccgag tgatcacgcc
     2101 cgagggccgc aacatcgccg gcgagcccga cctgccgatg ccgcccgacg cgaccaacgg
     2161 atcctacgag gcgcagttca agggccagag ggtgcacgtg gtctcggtgc agtcgccgtg
     2221 cggctcctcg gcatgcaaca tcctcgtcgc cgagaccacg gtgaagcgca cgcgcctgac
     2281 gcgcgagatc ctgttctcca gcctgatgcc gcagatggtg ctcgccgtgg cgacgctggt
     2341 catcgtctgg ttcggcgtca agcgcggcct cggtccgctc gcgcggctct ccgaagagat
     2401 caaggggcgc tcaccgcgcg acctgcaccc gatcgacgct gccgccgcgc cggaggagac
     2461 gcggccgctg ctcgcggcgc tgaacggcct gctcgagcag gtctccgagg cgagccgcaa
     2521 ccagcagcgc tttctggcga acgcggcgca ccagct
//
LOCUS       DASYJA010000335         1962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1773936, whole genome shotgun sequence.
ACCESSION   DASYJA010000335 DASYJA010000000
VERSION     DASYJA010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1773936"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="VGO02_07390"
     CDS             complement(<1..1004)
                     /locus_tag="VGO02_07390"
                     /inference="COORDINATES: protein motif:HMM:NF023683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV7477544.1"
                     /translation="MNTTARYVAAFLLSVLAPLAAGVEVVSNKMSPSDRLTVFDGSQN
                     TWRRVNLPTPADCVSSPAKALQCQDVAVLNTLDGFNIQPRLSIPFSGPIDVSSVNSDS
                     VFLVSLGSTLGWGSFGDKVGINQVVWDTATNTLHVESDELLEQHTRYALVVTNRVRDA
                     SGAPVMAAKKRHADDDDDNDKIEKSAQRHGVRIVGASIFTTQSTTSTLQKVHAQINAA
                     SPARARFTLGAAGERTVFPLGSLTAIQYNLQTGTAPVFDPKRLPLEALGVIPGAVGNI
                     AFGSYASPDYNVSPGEFIPPTGTRTGVPAVQRMNTIYFTLFLPAGSRPAAGWPVAIWG
                     "
     gene            complement(1089..>1962)
                     /locus_tag="VGO02_07395"
     CDS             complement(1089..>1962)
                     /locus_tag="VGO02_07395"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674285.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEV7477545.1"
                     /translation="RDGQQVQVGEVLARIPQEVSKTRDITGGLPRVAELFEARSPKDA
                     GMLAEVTGTVSFGKDTKGKQRLVITDLEKQEHEYLIAKDKHLLVHDGQVVNKGEMIVD
                     GPVDPHDLLRLLGIEELARYVIDEVQDVYRLQGVKINDKHIEVIVRQMLRRVEITDPG
                     ETTYITGEQSERAELFEENEKAVKEGKKPAQYANMLLGITKASLSTDSFISAASFQET
                     TRVLTEAAIMGKRDTLRGLKENVIVGRLIPAGTGLAYHNIRKAQAAEPVLAELPLAAP
                     ETTAPQAEVTTGGS"
BASE COUNT          273 a          639 c          676 g          374 t
ORIGIN      
        1 tggccccaga tcgccaccgg ccagccggcc gcaggccggg agccggccgg caggaacagg
       61 gtgaagtaga tcgtgttcat gcgctgcact gccgggacgc cggtgcgggt gccggttggc
      121 gggatgaact cgccgggcga cacgttgtag tcgggcgagg cgtagctgcc gaaggcgatg
      181 ttgccgaccg cgccggggat cacgccgagc gcctccagcg gaaggcgctt cgggtcaaag
      241 actggcgcgg taccggtctg caggttgtac tggatcgcgg tcaggctgcc gagcgggaag
      301 acggtgcgct cgccggccgc tccgagggta aaccttgccc gggccggcga ggcggcattg
      361 atctgcgcgt ggaccttctg cagcgtcgag gtggtgctct gcgtggtgaa gatgctggcg
      421 ccgacgatgc gcacgccatg gcgctgcgcc gatttctcga tcttgtcgtt gtcgtcgtcg
      481 tcatcggcgt ggcgcttctt tgccgccatg accggcgcgc cgctcgcgtc gcgcacacgg
      541 ttggtcacca ccagcgcata gcgggtgtgc tgctcgagca gctcgtcgga ctccacgtgc
      601 agggtgttgg tggccgtgtc ccagaccacc tggttgatgc cgaccttgtc gccgaaggag
      661 ccccagccca gcgtgctgcc gaggctgacc aggaacacgc tgtcgctgtt gacgctcgag
      721 acgtcgatcg gcccgctgaa cgggatcgac agccgcggct ggatgttgaa cccgtccagg
      781 gtgttcagca ccgcaacgtc ctggcattgc agcgccttcg ccggcgagct cacgcagtcg
      841 gccggggtgg ggaggttcac ccgtcgccag gtgttctggc tgccgtcgaa tacggtgagc
      901 cggtcgctcg gggacatctt gttggagacg acttctacgc ccgcggcgag cggggcaagg
      961 acggacagca ggaatgcagc gacatacctg gcggtggtat tcatgactcc ctcctctgcc
     1021 ggccataata aaagaaaggg ccgcccttgg gcggcccttt cagtcgtgcc gttgatgcaa
     1081 gctacaggtc aggatccgcc ggtagtgacc tccgcctgcg gcgcggtggt ttccggcgcg
     1141 gccagcggca gctcggcgag caccggctcg gctgcctggg ccttgcggat gttgtggtac
     1201 gcgaggcccg ttcccgccgg gatcagccgg ccgacgatca cgttctcctt caggccgcgc
     1261 agtgtgtcgc gcttgcccat gatcgccgcc tcggtcagca cgcgcgtggt ctcctggaag
     1321 gaggccgcgg agatgaacga gtcggtcgac agggacgcct tggtgatgcc gaggagcatg
     1381 ttggcgtact gcgcgggctt cttgccctct ttcaccgcct tttcgttctc ctcgaacagc
     1441 tcggcgcgct ccgactgctc gccggtgatg tacgtggtct cgcccggatc ggtgatttcc
     1501 acgcggcgca gcatctggcg cacgatcacc tcgatgtgct tgtcgttgat cttcacgccc
     1561 tgcagacggt acacgtcctg cacctcgtcg atgacgtagc gcgccagctc ctcgatgccg
     1621 agcaggcgca gcaggtcgtg cggatccacc gggccgtcga cgatcatctc gcccttgttg
     1681 actacctggc cgtcgtgcac cagcaggtgc ttgtccttgg cgatcaggta ctcgtgctcc
     1741 tgcttctcga ggtcggtgat caccaggcgc tgcttgccct tggtgtcctt gccgaagctc
     1801 acggtgccag tgacctctgc gagcatgccc gcgtccttcg gcgagcgcgc ttcgaagagc
     1861 tccgccaccc gcggcaggcc gcccgtgatg tcgcgtgtct tggagacctc ctgcgggatg
     1921 cgcgccagca cttcgccgac ctgcacctgc tggccgtcgc gc
//
LOCUS       DASYJA010000336         4457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_958987, whole genome shotgun sequence.
ACCESSION   DASYJA010000336 DASYJA010000000
VERSION     DASYJA010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958987"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2217)
                     /locus_tag="VGO02_07400"
     CDS             complement(<1..2217)
                     /locus_tag="VGO02_07400"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEV7477546.1"
                     /translation="MDIQVEAPEQVISRDTLLEKYAKGDESSIGEVRRRVARALAQPE
                     APDKRAYWEGRFLQAQEDGFIPAGRINSAAGVQLQATLINCFVQPVGDSISETVDGRP
                     GIYAALQEAAETMRRGGGVGYDFSAIRPKGAEVKGTHSRASGPVSYMRVFDRSCETVE
                     SAGSRRGAQMGVLRCDHPDIEEFIHAKDRGDLSNFNISIGVTDEFMVAVEKDSLFSLI
                     HKAKPWPTSETRQREDGMWIYRNVRARELWHQVMRSTYDHAEPGILFLDRMNLDNNLR
                     YCETIEATNPCAEQPLPPYGCCCLGSVDLTKYVKNPFTEKSSFDFEHFGRTVETSIRM
                     LDNVLEVTAWPLEQQKKEAMAKRRVGLGFTGLGDALIMLRLRYDTAEARAMAARISEV
                     MRDHSYRASAELAKEKGEFPLFNADLYLSGTSFATRLPENIKNEIRKKGLRNSHLLSI
                     APTGTISLAFADNASNGIEPPFSWTYTRKKRMADGTMQEFPVEDHAWRRYKAQGGDVN
                     ALPPYFVTALELSAHAHEQMVASVAPYIDTSISKTVNVPEDYPYAEFQHLYLHAWKSG
                     LKGLATYRPNKILGSVLSVSTPPQDFVQDDFNRRLSIKSVPAPVLSSLRWPGRPDLSG
                     GNPAWTYMIEHPQYRFAVFVGHVENGAPKPFEVWVNGSEQPRGLGALAKTLSMDMRAK
                     DLGWLRLKLDALAKAGGDAFDMPFPPGGESKRMPSLVAAFAQIVRWRVEQLASGLSD"
     gene            complement(2257..2982)
                     /locus_tag="VGO02_07405"
     CDS             complement(2257..2982)
                     /locus_tag="VGO02_07405"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEV7477547.1"
                     /translation="MAAERQELDFLTKLRIRELILMGLITITAVLANLPQEYVEETLG
                     VSPTTLLAVLAIAVIIGLFLYLKFFFFMAVVLLIAGANLPEQIADGLNISKIPILLAL
                     IALVGIAAINYFVKLMPTGLEPKPREKSLEGVKALFYAIEKNNLVYAQKVLSMHFDPN
                     LHHDNGYTPLAYAAMKGSQPMVELLLRNGADPTQTTKEGDTPVELALRFSHGEVADLL
                     KRARQSAEPATAGIRAAASQAAA"
     gene            3055..3594
                     /gene="greB"
                     /locus_tag="VGO02_07410"
     CDS             3055..3594
                     /gene="greB"
                     /locus_tag="VGO02_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEV7477548.1"
                     /translation="MSKAFVKETEHEEPDEEREPALPAGGKNYISLAGFTRLKAELKQ
                     LVELERPEVVRTVSWAASNGDRSENGDYIYGKKRLREIDRRVRYLIKRLENAEVVDAS
                     GRDTDQVFFGATVKITLAGRTQELTIVGADEVDPAQGRVSWISPIAKALIKAREGDTV
                     LLRSPAGEEKVEILEVRYL"
     gene            3591..3962
                     /gene="mscL"
                     /locus_tag="VGO02_07415"
     CDS             3591..3962
                     /gene="mscL"
                     /locus_tag="VGO02_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEV7477549.1"
                     /translation="MSIVKEFREFAVKGNVVDLAVGVIIGAAFGKIVASLVGDIVMPF
                     VGTLIGGVDFSSLSVQFRDAKIQYGKFLQTCVDFLIITWAIFVAIKVINRMQHETPPA
                     PPTPPRQEQLLEEIRDLLKKK"
     gene            complement(4090..>4457)
                     /locus_tag="VGO02_07420"
     CDS             complement(4090..>4457)
                     /locus_tag="VGO02_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV7477550.1"
                     /translation="SSAFDESASFRISSIFALENRARVESAIREELSRAAREGFSAAE
                     VEAGRKSLLEARRLSRTQDRALASRLGSYLFAGRTFAWDADFEKRIAALTPAEINAAL
                     KRNIDPERLSVVTAGAFKK"
BASE COUNT          750 a         1389 c         1515 g          803 t
ORIGIN      
        1 gtccgacaaa ccggaagcca gctgttcgac acgccagcgc acgatctgcg cgaacgcggc
       61 gaccagcgac ggcatgcgct tgctctcgcc gccgggcggg aagggcatgt cgaaggcgtc
      121 gccgcccgcc ttggcgaggg cgtcgagctt gaggcgcagc caacccaggt ccttggcgcg
      181 catgtccatc gacagcgtct tggcgagcgc gcccaggccg cgcggctgct cgctgccgtt
      241 cacccagacc tcgaagggct tgggcgcgcc gttctccaca tggccgacga acaccgcgaa
      301 gcggtattgc gggtgctcga tcatgtaggt ccaggccggg ttgccgccgg agaggtccgg
      361 ccggcccggc cagcgcaggc tcgagagcac cggcgcggga acggacttga tcgacagccg
      421 ccggttgaaa tcgtcctgca cgaagtcctg cgggggcgtg ctcaccgaca ggacagaacc
      481 caggatcttg ttcggccgat aggtcgcgag gcccttcagc cccgacttcc aggcgtgcag
      541 gtagaggtgc tggaactcgg cgtacgggta gtcctcggga acgttgaccg tcttcgagat
      601 gctggtatcg atgtacggcg cgaccgacgc gaccatctgc tcgtgcgcgt gcgcggagag
      661 ctcgagcgcc gtcacgaagt agggcggcag cgcgttcacg tctccgccct gcgccttgta
      721 gcgccgccag gcgtggtcct cgaccgggaa ttcctgcatc gtgccgtcgg ccatgcgctt
      781 cttgcgcgtg taggtccagg agaacggcgg ctcgatgccg ttggaggcgt tgtcggcaaa
      841 ggccaggctg atggtgccgg tcggcgcgat cgacagcaga tgggagtttc tcaggccttt
      901 ttttcttatt tcatttttta tgttttcagg cagccgcgtg gcgaagctgg tcccggacag
      961 gtagaggtcc gcgttgaaca gcggaaactc gcctttttct ttcgccagct cggccgacgc
     1021 tcggtaggag tggtcgcgca tgacctccga gatgcgcgcc gccatggcgc gcgcctcggc
     1081 cgtgtcgtag cgcaggcgca gcatgatcag cgcgtcgccc agcccggtga agcccaggcc
     1141 gacgcggcgc ttggccatcg cctccttctt ctgctgctcg agcggccagg cggtgacctc
     1201 gagcacgttg tcgagcatgc ggatcgaagt ctcgaccgtc ctgccgaaat gctcgaaatc
     1261 aaaagatgat ttttccgtga atgggttctt tacgtatttc gtgagatcaa ccgaacccaa
     1321 acagcaacac ccatagggcg gcagcggctg ctcggcgcac ggattggtcg cctcgatcgt
     1381 ctcgcaatac ctgaggttgt tgtcgaggtt catgcggtcg aggaacagga tgccgggctc
     1441 ggcgtggtcg taggtcgagc gcatcacctg gtgccacagc tcgcgcgccc gcacgttcct
     1501 gtagatccac atgccgtctt cgcgctggcg cgtttcggac gtcggccagg gctttgcttt
     1561 gtgtatcaag gaaaaaagag agtccttttc gaccgcgacc atgaactcat cggtgacacc
     1621 gatggaaatg ttgaaattcg acaggtcgcc gcggtccttg gcgtggatga attcctcgat
     1681 gtcggggtgg tcgcagcgca gcacgcccat ctgcgcgccg cggcgcgagc cggccgactc
     1741 gaccgtctcg caggagcggt cgaagacgcg catgtaggac actgggccgc tcgcgcgcga
     1801 gtgcgtgccc ttgacctcgg cgcccttggg ccggatggcg gagaagtcgt agccgacgcc
     1861 gccgccgcgg cgcatggtct cggcggcctc ctgcagcgcg gcgtagatgc cgggccggcc
     1921 gtcgacggtc tccgagatcg aatcgccgac cggctgcacg aagcagttga tcagggtggc
     1981 ctgcagctgc acgccggcgg cggagttgat gcgcccggcg ggaatgaagc cgtcctcctg
     2041 cgcctgcagg aagcggcctt cccagtaggc gcgcttgtcg ggggcttcgg gctgggcgag
     2101 ggcgcgcgct acccggcggc gcacttcgcc gatgctgctt tcgtcgccct tcgcgtattt
     2161 ctcgagcagg gtgtcgcgtg aaatgacctg ctcaggcgct tcgacctgaa tatccataca
     2221 gtaccgatta taactgctct tgcgggtttg tccgagctac gcggcggctt gcgaagcggc
     2281 tgcgcggatg ccggctgtag ccggctcggc gctctggcgg gcgcgcttga ggaggtcggc
     2341 gacctcgccg tgcgagaagc gcagcgccag ttcgaccggc gtatcgcctt ccttggtggt
     2401 ctgcgtcggg tcggcgccgt tcctgagcag cagctccacc atcggctggc tgcctttcat
     2461 cgcggcatag gcgagcgggg tgtagccgtt gtcgtggtgc aggttgggat cgaaatgcat
     2521 cgacagcacc ttctgcgcgt acaccaggtt gttcttctcg atggcgtaga acagcgcctt
     2581 gacgccctcg aggctctttt cccgcggctt gggctccagg ccggtgggca tcagcttgac
     2641 gaagtagttg atcgccgcga tgcccaccag ggcgatcagg gcgagcagga tcgggatctt
     2701 ggagatgttg aggccgtcgg cgatctgctc gggcaggttg gcgccggcga tcagcagcac
     2761 caccgccatg aagaagaaga acttgaggta gaggaacagg ccgatgatca ccgcgatggc
     2821 gagcaccgcg agcagcgtgg tcgggctgac gccgagcgtc tcctcgacgt attcctgcgg
     2881 caggttggcg agcaccgcgg taatggtgat caggcccatc aggatcagct cgcggatgcg
     2941 cagcttcgtc aggaaatcga gctcttgcct ctcagccgcc atcttgttct ccccttgagg
     3001 tgccaattct ggaagctcgg ccccccgctt gcaagggcta aaatcgacct tgccatgtcc
     3061 aaggcgtttg tcaaggaaac ggagcacgaa gagccagacg aggagcgcga gccggcgctg
     3121 cccgctggag gaaagaatta catcagcctg gcgggcttca cgcggctgaa agccgagctg
     3181 aagcagctgg tggagctcga gcgccccgag gtggtgagga ccgtctcctg ggcggcctcc
     3241 aacggcgacc gctcggagaa cggcgactac atctacggca agaagcggct gcgcgagatc
     3301 gaccggcgcg tgcgctacct catcaagcgg ctggagaacg ccgaggtggt ggatgcctcc
     3361 ggccgcgaca ccgaccaggt gttcttcggc gccacggtta agatcacgct ggcgggccgc
     3421 acgcaggagc tgaccatcgt cggcgcggac gaggtcgatc cggcgcaggg cagggtatcg
     3481 tggatctcgc cgatcgcgaa ggcgctcatc aaggcacgcg agggcgatac cgtcttgctg
     3541 cgcagtcccg cgggtgagga aaaagtcgaa attctcgagg tgaggtacct atgagcatcg
     3601 tcaaggagtt ccgcgagttc gcggtcaagg gcaacgtcgt cgaccttgcc gtcggcgtga
     3661 tcatcggcgc cgccttcggc aagatcgtcg cctcgctggt cggcgacatc gtcatgccgt
     3721 tcgtcggcac cctgatcggc ggcgtcgact tcagcagcct gagcgtgcag ttccgcgacg
     3781 cgaagatcca gtacggcaag ttcctgcaga cctgcgtcga cttcctgatc atcacctggg
     3841 cgatcttcgt cgccatcaag gtgatcaacc gcatgcagca cgagacgccg cccgccccgc
     3901 cgacaccgcc gcgccaggag cagcttttgg aagagatcag ggatcttctg aagaagaaat
     3961 aaatcggggt cagagcccga tttctctttg ccccctgaca agggggaagc gaaaaaccaa
     4021 ccccccgttc gcctgcagct cacgaccccc ttgtcagggg gtcgaagcaa ttcgtgctct
     4081 gacccggatt tatttcttga acgcgccggc ggtgaccacc gacaggcgct ccggatcgat
     4141 gttgcgcttg agcgcggcgt tgatctcggc gggggtcagg gcggcgatgc gcttctcgaa
     4201 gtcggcgtcc caggcgaagg tgcgcccggc gaagaggtag gagccgagcc ggctggcgag
     4261 cgcgcggtcc tgggtgcgcg acaggcgccg cgcctcgagc agcgacttcc tgccggcttc
     4321 gacttccgcc gcgctgaagc cctcgcgcgc ggcgcgcgac agctcctcgc ggatcgcgct
     4381 ctcgacgcgc gcgcggttct ccagcgcgaa gatcgacgag atgcggaacg acgccgactc
     4441 gtcgaacgcg ctcgagg
//
LOCUS       DASYJA010000337         5577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_869063, whole genome shotgun sequence.
ACCESSION   DASYJA010000337 DASYJA010000000
VERSION     DASYJA010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869063"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VGO02_07425"
     CDS             <1..280
                     /locus_tag="VGO02_07425"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HEV7477551.1"
                     /translation="RRLRRGEPFGRLHVLFVGQAEAARLPEILATAKGHGVLTVTETD
                     NALAQGSMINFVAVEQKVRFDVALPPAERDKLKISARLLAVARKVVTG"
     gene            280..1815
                     /locus_tag="VGO02_07430"
     CDS             280..1815
                     /locus_tag="VGO02_07430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV7477552.1"
                     /translation="MTWIRNSVAGKLMSVVLLATLAAVVFTIAAMLAYDLSSYHRSWV
                     ADISTQADLLGRTVAPALAFDDPRVARENLELLRSRPKVRAAAVYNARGRLFATYAAA
                     GEAGFPELPEADGARIAGDDLIVFKRIVDGREILGTVYVRADYELYDRLLGYLGIASI
                     VAIAALLVAYALSLWMQRAVMRPLLAISEVARQVAGQRNYALRAEKLSSDEVGMLAEA
                     FNAMLTDIGEATRGLEASKRDAEREVAERRRAEEEVLRLNAELEQRVKERTAQLEYTN
                     GELESFCYTVSHDLRAPLRAIDGFSQALLEDFPKDVPEEAQRYLGRIRSSTLRMGQLI
                     EDLLNLSRVSRVELARREIDFGEVARQVATDLHVRDPARAVEIVVWDDMPANADPRLL
                     RAALENLMGNAWKFTGKVERPRIEVGALRDGERSTFFVKDNGAGFDMAYANKLFGAFQ
                     RLHSEREYAGTGIGLATVQRIVHRHGGRIWADAKIGKGAVFFFTLEPGAAVDAPAPER
                     MQA"
     gene            1812..3914
                     /locus_tag="VGO02_07435"
     CDS             1812..3914
                     /locus_tag="VGO02_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEV7477553.1"
                     /translation="MSPGDHPVNVLFVEDSEDDVALELRALERDGLQILPRRVDSEAT
                     LREALATMLPDAILSDFSMPGFDGLQALKLSRELAPRVPFIFVSGTIGEERAIEAIRL
                     GATDYVLKNNLRRLSTSVRRALAEASERGRVRVAEAERARLVEIMEATSDYVGMSDPD
                     GRQIYLNAAGRKMVGIPENEVPGKRILEIYPAWARRIIEEEARPAAARDGIWQGETAM
                     LAPDGSEVPVSQVVIAHRNPEGGVRFYSAIARDISERKAYEARIQHLANYDALSGLPN
                     RSLLADRASQVISHARRHQRVCGLIALNVDRFKLLNESYGHAAADVLLKAIGERLAGA
                     VRDGDTVARLGADTFAALASSLARPEDVLSVVRKIQQAARTPFSIEGREVHIDLSIGA
                     SVFPRDGEEFDLLLRNADAAMNRVKAAGGGGFQFYAAAMTKEATDRVEIEGALHGAAA
                     RGELQLHYQPQVELTTGRAIAVEALMRWTHPQRGAVPPGQFVPIAEQSDLIVSLGEWA
                     LQESCRQLRLWPDPGLRIAVNVSPRQFRSPGFAETVARALREHGVEGRRLEIELTEGV
                     LIEDRDEAVAILERLKQIGVQIAIDDFGTGYSSLSYLSGLPIDCLKIDRAFVTRIGKG
                     GRDAAIAQAIISLAHSLGMRVLAEGVETAQQADFLRAHGCDEAQGYLFARPCAAHDAA
                     RLIAADSLPMAAATEERR"
     gene            3911..4348
                     /locus_tag="VGO02_07440"
     CDS             3911..4348
                     /locus_tag="VGO02_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7477554.1"
                     /translation="MSEKMILLVEDNPDDEELTTRALRQAKVANDLVVARDGAEALEY
                     VFGTGAHAGRDLSRMPALILLDLKLPKLSGLDVLQQLRADERSKLIPVVILTSSSEDE
                     DMVKSYRHGANSYVRKPVEFNAFATAVSQLGVYWMLINQPPPR"
     gene            complement(4356..>5577)
                     /gene="boxB"
                     /locus_tag="VGO02_07445"
     CDS             complement(4356..>5577)
                     /gene="boxB"
                     /locus_tag="VGO02_07445"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEV7477555.1"
                     /translation="TFGYVKMPEYRWGIFLAEREADRRIGFGDDVGREVWQQVPGEYR
                     ATLRRLIVTQGDTEPASVEQQRLLGHTCPSLYDLRNLFQINVEEGRHLWAMVYLLHAY
                     FGRDGREEAEELLARHSGDADKPRILTTFNEPISDWLSLFCFTYFTDRDGKYQLKSLA
                     ESAFDPLSRTCRFMLTEEAHHMFVGETGLGRVVRRTLEVMKEIGTDDPAKVRAAGAID
                     LPTLQKYLNFWCSSSLDLFGSEISSNSAANFANGLKGRPDESQYDDHVLRDKIMKMKT
                     PAGEEDVPMLNALNEVMRESYIKDCEIGLKRWNRVIERAGHATRLTLPASSFRRSIGA
                     WAGQPVSPEGKMVSKEEYEKQLFGWIPSQADRAFVHSLMKRVVEPGKMAAWIAPPERG
                     INNLPVEYEYVKLH"
BASE COUNT          875 a         1876 c         1961 g          865 t
ORIGIN      
        1 ccgcaggctg cgccgcggcg agcccttcgg gcgcctgcac gtgctgttcg tcggccaggc
       61 cgaggcggcg cggctgccgg agatcctcgc taccgccaag gggcacgggg tgctcacggt
      121 caccgagacc gacaacgccc tggcgcaggg cagcatgatc aatttcgtcg ccgtcgagca
      181 gaaggtgcgc ttcgacgtgg cattgccgcc cgccgagcgc gacaagctga agatcagcgc
      241 gcgcctgctg gcggtggcgc gcaaagtggt gaccggctga tgacctggat ccgcaattcg
      301 gtcgccggca agctgatgag cgtggtgctg ctcgccaccc tggcggcggt cgtcttcacg
      361 atcgcggcga tgctggccta cgacctgagc agctaccacc gcagctgggt cgccgacatc
      421 agcacgcagg ccgacctgct cggacggacc gtggcgccgg cgctcgcctt tgacgaccct
      481 cgcgtcgcca gggaaaatct cgagctgctg cgctcgcggc cgaaagtgcg cgccgcggcg
      541 gtctacaacg cccgcggcag gctctttgcg acctacgccg ccgccggcga ggcgggcttc
      601 ccggagctgc ccgaggccga cggcgcgcgt attgcaggcg acgacctgat cgtgttcaag
      661 cgcatcgtcg atggccggga gattctcggc accgtctacg tgcgcgccga ctacgagctc
      721 tacgaccggc tgctcggcta cctcggcatc gccagcatcg tggcgatcgc ggcgctgctg
      781 gtggcctacg cgctttcgct ctggatgcag cgcgccgtga tgcgtccgct gctcgccatc
      841 tcggaggtgg cccgccaggt cgccggccag cgcaactatg cgctgcgcgc ggagaagctg
      901 tcgagcgacg aggtcggcat gctggccgag gcgttcaacg ccatgctgac cgatatcggc
      961 gaggcgacgc gcgggctgga ggcatccaag cgcgacgcgg agcgcgaggt ggccgagcgc
     1021 cggcgcgccg aggaggaggt cctgcggctc aacgccgagc tcgagcagcg cgtcaaggag
     1081 cgcaccgcgc agctcgagta caccaacggc gagctggagt cgttctgcta taccgtgtcg
     1141 cacgacctgc gcgcgccgct gcgcgcgatc gacggcttca gccaggcgct gctcgaggat
     1201 ttccccaagg acgtccccga ggaggcgcag cgctacctgg gccgcatccg ctcctcgacg
     1261 ctgcgcatgg gccagctgat cgaggacctg ctgaatctgt cgcgcgtgtc gcgcgtcgag
     1321 ctggcgcggc gcgagatcga tttcggcgag gtggcgcgcc aggtggccac cgacctgcac
     1381 gtgcgcgacc cggcgcgcgc cgtcgagatc gtggtgtggg acgacatgcc ggcgaacgcc
     1441 gatccgaggt tgctgcgcgc ggcgctcgag aacctgatgg gcaacgcctg gaaattcacc
     1501 ggcaaggtcg agcggccgcg catcgaggtc ggcgcgctgc gcgacggcga acgcagcacc
     1561 ttcttcgtca aggacaacgg ggcggggttc gacatggcct acgccaacaa gctgttcggc
     1621 gccttccagc gcctgcactc ggagcgcgaa tacgccggca ccggcatcgg tctggccacc
     1681 gtgcagcgca tcgtgcaccg ccacggcggc aggatctggg ccgacgcgaa gatcggaaaa
     1741 ggcgcggttt tcttcttcac gctggagccc ggcgcggcgg tagacgcgcc ggcccctgaa
     1801 aggatgcagg catgagtccc ggcgaccacc cggtcaacgt gctgttcgtc gaggattcgg
     1861 aagacgacgt cgcactcgag ctgcgcgcgc tggagcgcga cggcctgcag atcctgcccc
     1921 ggcgggtcga ttcggaagcc accctgcgcg aggcgctcgc caccatgctg cccgacgcca
     1981 tcctgtcgga cttctcgatg cccggcttcg acgggctgca ggcgctcaag ctctcgcgcg
     2041 agctcgcgcc gcgcgtgccg ttcatcttcg tctccggcac catcggcgag gaacgcgcga
     2101 tcgaggcgat ccgcctgggc gccacggact acgtgttgaa gaacaacctg cgccgcctgt
     2161 cgacttcggt gcggcgcgcg ctggcggagg cgagcgagcg cggacgcgta cgggtggcgg
     2221 aggcggagcg cgcgcgccta gtggagatca tggaggcgac cagcgactac gtcggcatgt
     2281 cggatcccga cggcaggcag atctatctca acgccgccgg ccgcaagatg gtcggcattc
     2341 ccgagaacga ggtccccggc aagcgcatcc tggagatcta tccggcctgg gcgcgccgca
     2401 ttatcgagga ggaggcgcgg ccggccgcgg cgcgcgacgg tatctggcag ggcgagaccg
     2461 caatgctcgc ccccgacggc agcgaggtgc cggtttccca ggtcgtcatc gcgcaccgca
     2521 atccggaagg cggggtgcgc ttctactccg ctattgcccg cgacatcagc gagcgcaagg
     2581 cctacgaggc gcgcatccag catctcgcga attacgatgc gctgtcgggc ctgccgaacc
     2641 gcagcctgct cgccgaccgc gcctcgcagg tgatttccca tgcgcggcgc caccagcgcg
     2701 tgtgcggact gatcgcgctc aacgtcgatc gcttcaagct gctgaacgag agctacggcc
     2761 acgccgccgc cgacgtcctg ctgaaggcga tcggcgagcg cctcgcgggc gcggtgcgcg
     2821 acggcgacac ggtggcacgc ctgggcgccg ataccttcgc ggcgctcgcc agcagcctgg
     2881 cgcggcccga ggacgtgctc agcgtggtgc gcaagatcca gcaagcggcc aggacgccgt
     2941 tctccatcga ggggcgcgag gtgcacatcg acctgagcat cggcgcgagc gtcttcccgc
     3001 gcgacggcga ggagttcgac ctgcttctgc gcaacgccga cgcggcaatg aaccgcgtca
     3061 aggcggcggg cggcggcggc ttccagttct acgccgcggc gatgaccaag gaggcgaccg
     3121 accgcgtcga gatcgagggc gcgctgcacg gcgcggcggc gcgcggcgag ctgcagctgc
     3181 actaccagcc gcaagtcgag ctgacgaccg ggcgcgccat cgcggtcgag gcgctgatgc
     3241 gctggacgca cccgcagcgc ggcgcggtgc cgccggggca gttcgtgccg atcgccgagc
     3301 agtccgacct gatcgtctcg ctcggcgaat gggcgttgca ggagagctgc cgccagctgc
     3361 ggctctggcc cgacccgggg ctgcgcatag cggtcaacgt ctcgccgcgc cagttccgca
     3421 gcccgggctt cgccgagact gtggcgcgcg cgttgcgcga gcacggcgtc gagggtcggc
     3481 gcctcgagat cgagcttacc gagggcgtgc tgatcgagga ccgcgacgag gcggtggcca
     3541 tcctcgagcg cctgaagcag atcggcgtgc agattgcgat cgacgacttc ggcaccggct
     3601 actccagcct gagctacctt tcggggctgc cgatcgactg cctgaagatc gaccgcgcgt
     3661 tcgtcacccg catcggcaag ggcgggcgcg acgcggcgat cgcgcaggcg atcatctcgc
     3721 tcgcccactc gctgggcatg cgcgtgctcg ccgagggcgt ggagaccgcg cagcaggccg
     3781 atttcttgcg tgcgcacggt tgcgacgagg cgcagggcta cctgttcgcc cgcccctgcg
     3841 cggcgcacga cgcggcgcgc ctgatcgccg cagattccct tccgatggca gctgcaaccg
     3901 aggagcgacg atgagcgaga agatgatcct gctggtcgag gacaaccccg acgacgagga
     3961 gctcaccacc cgcgcgctgc gccaggcgaa agtcgccaac gacctggtgg tggcgcgcga
     4021 cggcgcggaa gcgctcgagt acgtgttcgg caccggcgcg cacgccggcc gcgacctgtc
     4081 gcgcatgccg gcgctgatcc tgctcgacct caagctgccc aagctcagcg gcctcgacgt
     4141 gctgcagcag ttgcgcgccg acgagcgttc caagctgatc ccggtggtca tcctcacctc
     4201 ttcgagcgag gacgaggaca tggtgaagag ctaccgccat ggcgccaaca gctacgtgcg
     4261 caagcccgtg gagttcaacg ccttcgccac agcggtgagc cagctcggcg tctattggat
     4321 gctgatcaac cagccgccgc cgcgctgagc gcggctcaat gtagcttcac gtactcgtat
     4381 tcgaccggca ggttgttgat gccgcgctcg ggcggcgcga tccaggccgc cattttcccc
     4441 ggctcgacga cgcgcttcat cagcgaatgt acgaaggcgc ggtcggcctg cgacgggatc
     4501 cagccgaata gctgcttctc gtattcttcc tttgaaacca tcttgccttc cgggctgacc
     4561 ggctggccgg cccaggcgcc gatggagcgg cggaagctcg acgcgggcag ggtcaggcgg
     4621 gtggcgtggc cggcgcgctc gatcacccga ttccagcgct tgaggccgat ctcgcagtcc
     4681 ttgatgtagg actcgcgcat cacctcgttg agcgcgttca gcatggggac gtcttcctcg
     4741 cccgccggag ttttcatttt cattattttg tctctcaaga catggtcgtc atactgcgac
     4801 tcgtccgggc ggcccttcaa tccattggcg aagttggccg ccgaattgga cgagatctcg
     4861 gagccgaaga ggtcgagcga ggacgagcac cagaagttga ggtacttctg cagcgttggc
     4921 aggtcgatcg cgccggcggc gcgcaccttg gccgggtcgt cggtgccgat ttccttcatc
     4981 acctcgaggg tgcgcctgac cacgcggccc aggccggtct cgccgacgaa catgtggtgc
     5041 gcttcctcgg tgagcatgaa gcggcaggtg cgcgacagcg gatcgaacgc gctctcggcg
     5101 agacttttca gctggtactt gccgtcgcgg tcggtgaagt aggtgaagca gaagagcgac
     5161 agccagtcgg agatcggctc gttgaaggtc gtgaggatgc gcggcttgtc ggcgtcgccg
     5221 gaatggcgcg cgagcagctc ctcggcctcc tcgcggccgt cgcggccgaa gtaggcgtgc
     5281 aggagataga ccatggccca caggtggcgg ccttcctcga cgttgatctg gaacaggttg
     5341 cgcaggtcgt agagggaagg gcaggtgtgg ccgagcaggc gctgctgctc cacggaggcg
     5401 ggctcggtgt cgccctgcgt gacgatcagg cggcgcaagg tcgcgcgata ctcgcctggc
     5461 acctgctgcc atacttcgcg tccaacgtcg tcgccgaagc cgatccgccg gtccgcttca
     5521 cgttcggcga ggaatatgcc ccagcgatat tcgggcatct tcacatagcc gaacgtc
//
LOCUS       DASYJA010000338         1789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2773837, whole genome shotgun sequence.
ACCESSION   DASYJA010000338 DASYJA010000000
VERSION     DASYJA010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2773837"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1772)
                     /locus_tag="VGO02_07450"
     CDS             complement(3..1772)
                     /locus_tag="VGO02_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEV7477556.1"
                     /translation="MSTYAAPLKDMRFVLNELAGMAAVAKLPGYEEATPDTVDAILEE
                     ASKFATEILDPINYSGDQEGSIWKDGAVTTPKGFKQAYQQYVQGGWGALPFGPEWGGQ
                     GLPKLVATAVEEMLTASNMSFSLCPLLTQGAIHALELCGSDQLKQAYLGNMIQGTWTG
                     TMNLTEPQAGSDLALVRTKAERSGDHYRISGQKIFITYGEHDMAENILHLVLARTPDA
                     PEGVKGISLFLVPKFLVKPDGSLGERNPARCASIEHKLGIHASPTAVMVFDKAVGYLV
                     GEENKGLSYMFIMMNAARFSVGLEGVAIAERAFQRALAFAKERLQGKDLVEGGQTVPI
                     IRHPDVRRMLMLMKSQTEAMRALAYTTAAALDFSYKTQDKQHQAFVDLMIPVVKGWCT
                     ETGIEVASLGVQVHGGMGFIEETGAAQHLRDARITTIYEGTTGIQAMDLVGRKIAREG
                     GATARAWLSVLKGFDSEIGKSKNPDLQAIRASLAAGAQAVADGVDFIVANSGKDLKGV
                     FAGAVPFLKLMGIVAGGWQMARAALSAERLSSGDSNFYQAKIKTARFYAEHVLVQASG
                     LRDTVVKGAGGVMALSEDQFLTA"
BASE COUNT          292 a          611 c          556 g          330 t
ORIGIN      
        1 ctctaggcgg taagaaactg atcttcggag agcgccatca ccccgcccgc gcccttcacc
       61 accgtgtcgc gcaggcccga cgcctgcacc agcacgtgct cggcgtagaa ccgggcagtc
      121 ttgatcttcg cctggtagaa attcgaatca cctgaagaca atctttcagc cgacagcgcg
      181 gccctcgcca tctgccagcc gccggcgacg atgcccatca gcttgaggaa cggcaccgcg
      241 cccgcgaata cgcctttcag gtccttgccc gagttggcga cgatgaagtc gacgccgtcg
      301 gcgaccgcct gcgcgccggc ggccagcgag gcgcggatcg cctgcaggtc cggattcttg
      361 gattttccga tctcagaatc aaaacctttt aaaacagaaa gccaagcgcg ggcagtcgcc
      421 ccgccctcgc gggcgatctt gcgcccgacc aggtccatcg cctggatgcc ggtggtgcct
      481 tcgtagatgg tggtgatgcg cgcgtcgcgc aggtgctgcg cggcgccggt ctcctcgatg
      541 aagcccatcc cgccgtgcac ctgcacgccg agcgaagcca cctcgatgcc ggtctcggtg
      601 caccagcctt tcaccaccgg gatcatcagg tcgacgaagg cctgatgctg cttgtcctgc
      661 gtcttgtatg aaaaatccag agccgccgcc gtcgtgtagg ccagcgcacg catcgcttcg
      721 gtctgcgact tcatcagcat cagcatgcgg cgcacgtcgg ggtggcggat gatcggcacc
      781 gtctggccgc cctcgaccag gtccttgccc tgcagccgct ccttggcgaa ggcaagcgcg
      841 cgctggaagg cgcgctcggc gatcgccacg ccctcgagcc cgacgctgaa gcgcgcggca
      901 ttcatcatga tgaacatgta ggacaggccc ttgttctcct cgcccaccag gtagccgacg
      961 gccttgtcga acaccatcac cgcggtcggg ctggcgtgga tgccgagctt gtgctcgatc
     1021 gaggcgcagc gcgcggggtt gcgctcgccg agcgagccgt cgggcttgac caggaacttc
     1081 ggcaccagga acagcgagat gcccttgacc ccttcgggcg cgtccggcgt gcgcgcgagc
     1141 acgaggtgca ggatgttctc cgccatgtcg tgctcgccgt aggtgatgaa gatcttctgg
     1201 cccgagatgc ggtagtggtc gccggacctt tccgccttcg tcctgaccag cgccaggtcg
     1261 gagccggcct gcggctcggt gaggttcatg gtcccggtcc aggtgccctg gatcatgttg
     1321 ccgaggtagg cctgcttcag ctggtccgag ccgcagagct cgagcgcgtg gatcgcgccc
     1381 tgcgtcagca gcgggcagag cgagaacgac atgttcgacg cggtgagcat ctcctctacc
     1441 gcggtggcga ccagcttcgg caggccctgg ccgccccact cgggcccgaa cggcagcgcg
     1501 ccccatccgc cttgcacgta ctgctggtac gcctgcttga agcccttcgg cgtcgtgacc
     1561 gccccatcct tccagatcga gccttcctga tcaccagaat agttaatagg atcaagtatt
     1621 tccgttgcaa acttcgaggc ttcctcgagg atggcgtcga ccgtgtccgg cgtcgcttcc
     1681 tcgtagccgg ggagcttggc gactgccgcc atcccggcca gctcattgag gacgaaccgc
     1741 atgtccttca gaggggccgc ataggtactc atttcttcag ctcctcgac
//
LOCUS       DASYJA010000339         3634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1411771, whole genome shotgun sequence.
ACCESSION   DASYJA010000339 DASYJA010000000
VERSION     DASYJA010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411771"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /gene="argA"
                     /locus_tag="VGO02_07455"
     CDS             <1..526
                     /gene="argA"
                     /locus_tag="VGO02_07455"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEV7477557.1"
                     /translation="TRRVESSLLLELFTHEGVGTMINAEPVEKLRPARIEDVRGMLAL
                     IEPLEADGTLVKRSRELLESEIGNFQVVEHDGVIVGRAALYPFPEDKSAEFACLAVAP
                     DYRDAAYGERLLKACEERAKALKLRRLFALTTHAAHWFLEQGFRAGEPSALPSQRQAM
                     YNWRRNSKVFLKRL"
     gene            537..809
                     /locus_tag="VGO02_07460"
     CDS             537..809
                     /locus_tag="VGO02_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEV7477558.1"
                     /translation="MARMVNCVKLGREAEGMAFPPYPGPLGKRIWESVSKEAWAQWLK
                     QQTMLVNENRLNLADPKARKYLMEQTERHFFGGGADTASGYVPPQK"
     gene            complement(813..1511)
                     /gene="phoU"
                     /locus_tag="VGO02_07465"
     CDS             complement(813..1511)
                     /gene="phoU"
                     /locus_tag="VGO02_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV7477559.1"
                     /translation="MAEHTSKQFDTELENVRSSVLQMGGLVEEQITRAVEALITGEME
                     LIDRVIADDHRVNALEVGLDEACSQIIARRQPAAGDLRLLIAVVKTITDLERIGDEAA
                     KIARMAKLIHTAERLHMPRIDLSHVADRALAMLRQSLDAFARLDVSEAMRVVKQDQAV
                     DDEFRAVMRQLITFMMEDPRTITRSLEILFIAKAVERIGDHAKNMAEYIVYMVKGRDV
                     RHISIEEVEKEILE"
     gene            complement(1548..2216)
                     /gene="rpiA"
                     /locus_tag="VGO02_07470"
     CDS             complement(1548..2216)
                     /gene="rpiA"
                     /locus_tag="VGO02_07470"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEV7477560.1"
                     /translation="MTQDELKEAVAREALKHVVQDAVVGVGSGSTVNKFIDCLSEIKN
                     KIEGAVAASEASAERLKKHGIRVFDLNSVNELPVYVDGADEITAHLHMIKGGGGALTR
                     EKIVAAVARKFVCICDASKLVPVLGKFPLPLEVVPMARSHVGRELRRRGAHPVLREGF
                     KTDNGNLILDCHGLTLLDPPAMEAELNNIAGVVSNGIFARRPADVLLLAEPGGVRTVS
                     AGKA"
     gene            complement(2213..>3634)
                     /gene="lpdA"
                     /locus_tag="VGO02_07475"
     CDS             complement(2213..>3634)
                     /gene="lpdA"
                     /locus_tag="VGO02_07475"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEV7477561.1"
                     /translation="GPSAPAPSASADIECDVVVLGSGPGGYSAAFRAADLGLKTVLVE
                     RYPVLGGVCLNVGCIPSKALLHTAAVMDAARALADHGVAFAEPKVDLAKLRAFKDKVV
                     GKLTGGLGSMAKMRKVTVVTGVGSFLDRNTLQVDGKKTIRFTSAIIAAGSQAAKLPFL
                     PEDPRIFDSTGALELKSVPKRMLVIGGGIIGLEMGTVYSTLGTRLDVVEMMDGLMLGA
                     DRDLVAVWQKFNAKRFDNVFLGTKTSKVEASKDGLKVSFEGKPLQTYDAILVSVGRVP
                     NGRKIGADKAGVAVTERGFIETDSQMRTNVPHIYAIGDITKNPMLAHKAVHEGHVAAE
                     AAAGHKSHFDARVIPSVAYTDPEIAWVGLTEDEAKKSGASIGVAKFPWAASGRAIANA
                     RDEGFTKLIFDADNHRVLGGGIVGTGAGDLISELALAVEMGADATDIGKTIHPHPTLS
                     ESVGMGAELFEGVCTDLPPQRKK"
BASE COUNT          588 a         1207 c         1182 g          657 t
ORIGIN      
        1 cacccggcgg gtggagagct cgctgctgct cgagctgttc acgcacgagg gcgtcggcac
       61 gatgatcaac gccgagccgg tggaaaagct gcggccggcg cgcatcgagg acgtgcgcgg
      121 catgctggcg ctgatcgagc cgctggaggc cgacggcacg ctggtcaagc gcagccgcga
      181 gctgctcgaa tccgagatcg gcaacttcca ggtggtcgag catgacggcg tgatcgtcgg
      241 ccgcgccgcg ctctatccgt tccccgagga caagagcgcg gagttcgcct gccttgcggt
      301 ggcgccggac taccgcgacg ctgcctatgg cgagcggctc ctgaaggcct gcgaggagcg
      361 cgcgaaggcg ctcaagctgc ggcgcctgtt cgcgctaacg acgcacgccg cgcactggtt
      421 tctcgagcag ggctttcgcg ccggcgagcc ctcggcgctg ccctcgcagc gccaggcgat
      481 gtacaactgg cgccgcaact cgaaggtgtt cctcaagcga ctgtagaatt gcctgcatgg
      541 cacgaatggt gaattgcgtg aagctcggcc gcgaggccga gggcatggcg tttccgccct
      601 acccggggcc gctcggcaag cgcatctggg agagcgtgtc caaggaagcc tgggcgcagt
      661 ggctcaagca gcagaccatg ctggtgaacg agaaccggct gaacctcgcc gatcccaagg
      721 cgcggaaata cctgatggag cagaccgagc ggcacttctt cggcggcggc gccgataccg
      781 cctcgggcta cgttccgccg cagaagtaac cactactcga gaatttcctt ctcgacttcc
      841 tcgatggaga tgtgccgcac gtcgcggccc ttcaccatgt agacgatgta ctcggccatg
      901 ttcttggcat ggtcgccgat gcgctcgacc gccttggcga tgaacaggat ctcgagcgag
      961 cgcgtgatgg tgcgcggatc ctccatcatg aaggtgatga gctggcgcat caccgcgcgg
     1021 aactcgtcgt cgaccgcctg atcctgcttc accacgcgca tcgcctcgga aacgtccagg
     1081 cgggcgaacg cgtcgagcga ctggcgcagc atggcgaggg cgcggtcggc gacgtgcgaa
     1141 aggtcgatgc gcggcatgtg cagccgctcg gcggtgtgga tcagcttcgc catgcgcgcg
     1201 atcttcgccg cctcgtcgcc gatgcgctcc aggtcggtga tggtcttcac caccgcgatc
     1261 agcaggcgca ggtcgcccgc ggccggctgg cggcgcgcga tgatctggct gcaggcctcg
     1321 tccaggccca cctcgagcgc gttgacgcgg tggtcgtcgg cgatcacgcg gtcgatgagc
     1381 tccatctcgc cggtgatcag cgcctccacc gcgcgcgtga tctgctcctc gaccagcccg
     1441 cccatctgca gcacgctcga gcggacgttc tccagctcgg tgtcgaattg cttggacgta
     1501 tgttcagcca taacgcgcca ccctagcagc ctgatgtgac tgaaatgtta cgcctttccg
     1561 gcgctcaccg ttctgacgcc gccgggctcg gccagcagga gcacgtcggc cggcctgcgg
     1621 gcaaagatgc cgttgctcac cacgccggcg atgttgttga gctcggcctc catggccggc
     1681 gggtcgagca gcgtgaggcc gtggcagtcg aggatcaggt taccgttgtc ggtcttgaag
     1741 ccctcgcgca gcaccgggtg cgcgccgcgc cggcgcagct cgcggccgac gtggctgcgc
     1801 gccatcggca ccacctcgag cggcagcggg aacttcccca gcaccggaac aagtttggag
     1861 gcgtcgcaga tgcagacgaa cttcctcgcc accgcggcga cgatcttctc gcgcgtcagc
     1921 gcgccgccgc cgcccttgat catgtgcagg tgcgcggtga tctcgtcggc gccgtcgaca
     1981 tagaccggca gctcgttgac gctgttcagg tcgaacacgc ggatgccatg tttcttcagc
     2041 cgctctgcgc ttgcttcgga agcggcgacg gcgccttcaa ttttgttttt gatttcagat
     2101 aaacaatcaa taaatttgtt caccgtcgac cccgagccga cgcccaccac ggcgtcctgg
     2161 acgacgtgct tgagcgcttc cctcgcgacg gcttccttca gctcgtcttg cgtcatttct
     2221 tgcgttgggg tgggaggtcg gtgcacacgc cctcgaacag ctccgcgccc atgccgaccg
     2281 attcgctgag cgtcggatgc ggatggatgg tcttgccgat atcggtggcg tcggcgccca
     2341 tttcgaccgc gagggcaagc tcgctgatca ggtcgcccgc gccggtgccg acgattcccc
     2401 cgccgagcac gcgatggttg tcggcatcga agatcagctt ggtgaagccc tcgtcgcgcg
     2461 cgttggcgat ggcgcgcccg gaagccgccc aagggaactt ggcgacgccg atgcttgcac
     2521 cgctcttctt cgcctcgtcc tcggtgaggc ccacccaggc gatctcggga tcggtatagg
     2581 cgaccgacgg aatcacgcgg gcgtcgaagt gcgatttgtg gcctgcggcg gcttcagccg
     2641 ccacatgccc ttcatgcacc gccttgtgcg cgagcatcgg gttcttggtg atgtcgccga
     2701 tggcatagat gtgcggcacg ttggtgcgca tctgcgaatc ggtttcgatg aagccgcgct
     2761 cggtgaccgc gacccctgcc ttgtccgcgc cgattttcct gccgttgggc acccggccca
     2821 ccgataccag aatggcgtca taggtctgca gcggtttgcc ctcgaaggag actttcaagc
     2881 cgtccttgga cgcctctact ttcgaggtct tggttcccag aaagacgttg tcgaagcgct
     2941 tggcgttgaa tttctgccac accgccacca ggtcgcggtc ggcgccgagc atcaggccgt
     3001 ccatcatctc cacgacatcc aagcgagtac caagcgtcga gtacacggtg cccatctcca
     3061 ggccgatgat gccgccgccg atgaccagca tgcgcttggg cacgctcttc agctcgagcg
     3121 cgccggtgga atcgaagatg cgggggtctt cgggcaggaa cggcaacttc gccgcctgcg
     3181 aaccggcggc gatgatcgcg ctcgtgaagc ggatggtctt cttgccgtct acctggagag
     3241 tattcctgtc gagaaaactg ccgacgccgg tcaccacggt gaccttgcgc atcttggcca
     3301 tcgatcccag gccgcctgtc agtttcccta ctaccttgtc cttgaaggcg cgcagcttgg
     3361 cgagatccac cttcggctcg gcgaaggcga cgccgtggtc ggccagcgcg cgcgcggcat
     3421 ccatcaccgc cgcggtgtgc agcagcgcct tcgaaggaat gcagccaacg ttgaggcaca
     3481 cgccgccgag caccggatag cgctcgacga gcaccgtctt cagcccaagg tctgcggcgc
     3541 ggaacgccgc cgaatagcct cccggccccg agccaagcac gaccacgtcg cactcgatgt
     3601 ccgctgaagc ggatggcgcg ggcgccgatg gacc
//
LOCUS       DASYJA010000340         1699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2229610, whole genome shotgun sequence.
ACCESSION   DASYJA010000340 DASYJA010000000
VERSION     DASYJA010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2229610"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1425
                     /locus_tag="VGO02_07480"
     CDS             <1..1425
                     /locus_tag="VGO02_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011655609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEV7477562.1"
                     /translation="TLAWGIAIYFLFGNMELLGKYTGIPDIPPISIGGLKLNDGAKIY
                     YLVWVVALALLVASANLLDSRAGRAIRALRFRAVMAESFGVDTARLKSVVFLCAALFA
                     ALSGWLYAHFLRFVSPNAFNVNAGIEYLFMAVIGGASHVWGAVIGASVLTLLKEWLKD
                     LLPTRFGASGNYEAVVFGALMLLLLHRASSGLGPLLARRFIRPQRRAISLRAEPLPRR
                     EFPKEEFILKVHEAKKRFGGIIAVRELSFEMRRGEILGLIGPNGAGKSTMFNLITGVL
                     PCDGGQIDCRGRRIDGLAPTAIARLGIARTFQHVNLVGAMSVLENVAIGAHLRGRRGV
                     LAAMLRTDRAEERRLLAEAARQIERVGLGAHLHDAAGSLPLGKQRLVEIARALSADPA
                     LLLLDEPAAGLRYAEKQALADLLRRLRGEGMSILLVEHDMDFVMRLVDRLVVLDFGEK
                     LAEGLPQAIQADPKVMEAYLGSVQ"
     gene            1422..>1699
                     /locus_tag="VGO02_07485"
     CDS             1422..>1699
                     /locus_tag="VGO02_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006415001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7477563.1"
                     /translation="MSLLEVRDLRVFYRRVEGLHRASLKVEEGAIVTVIGPNGAGKST
                     LLGAVMGMLPCTGEVRFQGAALGAEAVESRVTRGLCLVPERHELFGEMT"
BASE COUNT          244 a          574 c          597 g          284 t
ORIGIN      
        1 accctcgcat ggggcatcgc catctatttc ctcttcggca acatggagct cctcggcaag
       61 tacaccggca ttccggatat accgccgatt tccattggcg gtctcaagct gaatgacggc
      121 gcgaagatct actaccttgt ctgggtcgtg gcgctggcgc tgctcgtcgc ctcggcgaac
      181 ctgctcgact cgcgcgccgg acgcgccatc cgcgcgctgc gcttccgcgc ggtgatggcg
      241 gagagcttcg gcgtcgacac ggcgcgcctg aagtcggtgg tgtttctctg cgcggcgctc
      301 ttcgccgcgc tctccggctg gctgtacgcg cacttcctgc gcttcgtcag ccccaacgca
      361 ttcaacgtca acgcggggat cgagtacctg ttcatggccg tgatcggcgg cgcgagccac
      421 gtctgggggg cggtgatcgg cgcctcggtg ctgacgctcc tcaaggaatg gctgaaggac
      481 ctgctgccca cccgtttcgg cgcgagcggc aactacgagg cggtcgtgtt cggcgcgctg
      541 atgctcctgc tcctgcaccg cgcctcgtcc ggcctgggtc cgctgctcgc gcgccggttc
      601 atcaggccgc agcggcgcgc tatttccttg cgtgccgagc cgcttccgcg gagggagttt
      661 ccaaaagaag aatttatttt gaaagtgcac gaagcgaaaa agcgtttcgg cggaatcatc
      721 gccgtacgcg agctgagctt cgagatgcgc cgcggcgaga tcctcgggct gatcgggccg
      781 aacggcgccg gcaagagcac catgttcaac ctcatcaccg gggtgctgcc ctgcgacggc
      841 gggcagatcg actgccgcgg ccggcgcatc gacggcctcg cgcccacggc catcgcccgc
      901 ctgggcatcg cgcgcacctt ccagcacgtg aacctggtag gcgcgatgtc ggtgctcgag
      961 aacgtcgcca tcggcgcgca cctgcgcggg cggcgcggcg tgctcgccgc gatgctgcgc
     1021 accgaccgcg cggaggagcg ccgcctgctg gccgaggcgg cgcgccagat cgagcgcgtc
     1081 ggcctcggcg cgcacctgca cgacgccgcg ggcagcctgc cgctcggcaa gcagcgcctg
     1141 gtcgagatcg cgcgagcgct gagcgccgat ccggcgctgc tcctgctgga tgagcccgcg
     1201 gccggcctgc gctacgccga gaagcaggcg ctcgccgatc tgctgcgccg gctgcgcggc
     1261 gaaggcatga gcatcctgct ggtcgagcac gacatggact tcgtcatgcg cctggtcgac
     1321 cgcctggtgg tgctggactt cggcgagaag ctggccgagg ggctgccgca ggcgatccag
     1381 gccgatccga aggtgatgga agcctacctc gggagcgtgc agtgagtctc ctcgaagtca
     1441 gggacctgcg cgtcttctac cgccgcgtgg aaggtctgca ccgcgcgagc ctcaaggtcg
     1501 aggagggcgc catcgtcacg gtgatcggcc cgaacggcgc cggcaagtcg acgctgctcg
     1561 gcgcggtgat gggcatgctg ccgtgcacgg gcgaagtgcg cttccagggc gctgcgctcg
     1621 gcgccgaggc ggtggagagc cgggtaacgc gcggcctttg cctagtgccg gagcggcacg
     1681 agctcttcgg cgaaatgac
//
LOCUS       DASYJA010000341         3557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1957134, whole genome shotgun sequence.
ACCESSION   DASYJA010000341 DASYJA010000000
VERSION     DASYJA010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1957134"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VGO02_07490"
     CDS             complement(<1..906)
                     /locus_tag="VGO02_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEV7477564.1"
                     /translation="MVTRERIAETAQLIARYVRRTPVIDPDGDGRRVFKLELLQHSGS
                     FKARGAFANLLTREVPAAGVVAASGGNHGAAVAYAAHKLGKPARIYVPTVASPAKIER
                     IRSYGAELVVTGERYADALAASEKWAADSGAMQIHAYDQEGALLGQGTVGMEFEEQES
                     LDTLLVAVGGGGLIGGIAAWYAGKVRIIGVEPVLAPTLARALEAGRPVDAPAGGIAAD
                     SLAPKRVGELMFPLAQKYVEKVVLVEDDAIIASQKALWDQLRIAAEAGGAAAYAALLS
                     KAYRPGKDERVGVLVCGGNTTAVNFE"
     gene            942..1256
                     /locus_tag="VGO02_07495"
     CDS             942..1256
                     /locus_tag="VGO02_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011548450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7477565.1"
                     /translation="MARVAPLAAALRALANERRLQILEWLRQPRRHFPPQVDGDLVED
                     GVCTLLIAEKLGVSQPTVSEHLRVLAAAGLIRGKPIKQWIFYKRDEAAIGEIKKVIQA
                     KI"
     gene            1259..2086
                     /locus_tag="VGO02_07500"
     CDS             1259..2086
                     /locus_tag="VGO02_07500"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEV7477566.1"
                     /translation="MPKPLVSTDELAAHLAQWRVFDCRHDLMKPDLGAQQYREAHIPG
                     ALFASLDRDLSAPKTGKNGRHPLPDQDSFVAWLGRQGVRPEDQVVCYDAANGSTAARL
                     WWMLRWVGHRSVAVLDGGLAKWLKEGRPLDAVVPQPKPSTYASKRTAGTLDAGAVAAN
                     LKNLFLLDARAPARYRGEQEPIDPVAGHIPGAKNRFNMDNVLPDGTFKQSDALKRDFL
                     AVLKNQKPEDVVHYCGSGVAACHNLLAMEIAGLPGGKLYPGSWSEWLADPARPRGKA"
     gene            2086..2775
                     /locus_tag="VGO02_07505"
     CDS             2086..2775
                     /locus_tag="VGO02_07505"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006164139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEV7477567.1"
                     /translation="MPQHIGIVACSAEGAALCYKTICTEGAELLGAHAHPEVSMHTPS
                     LADYMGLIYRGDWQGVGDLMLESAQKLSAIGADFLICPDNTIHQGLPYIERRSPLPWL
                     HIAESVAAQAAERGFRRLGLTGTRWLVDSAVYPEKLQARGMVCVRPSDAERDEINRII
                     MDELVYGVFKPDAIACFQRVMRQMKAQGCDAVILGCTEIPLVMSDANSPLPTLDSTRL
                     LARAALRRALR"
     gene            complement(2772..>3557)
                     /locus_tag="VGO02_07510"
     CDS             complement(2772..>3557)
                     /locus_tag="VGO02_07510"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV7477568.1"
                     /translation="PADKYPARDPKLRYKPPLAPLEDYLVHARALGFERFVFVQPSVY
                     LRDNSCMLDAMREMPGSRGVVDVDEDASDQELAKMHALGVRGVRINVSPVHPPEAGLA
                     LKLLARIEKLDARLAGIGWHLDFLLPGWLTDELMRKIGSLQCDCTLAHMGMFLARDGV
                     QQAGFQRLLDLLRNGRGKVWVKLTGVYRVSQAPGFADAAPLARALIEAAPDRLIWGSD
                     YPHLSFADKVNPMQLFELFRGWTRDEATRRRILVDNPAKLFGF"
BASE COUNT          589 a         1264 c         1150 g          554 t
ORIGIN      
        1 ttcgaaattg actgcggtgg tgtttccgcc gcagaccagc acacctactc tctcgtcttt
       61 tcccggccga taagccttcg acagcagcgc cgcatacgcg gccgctccgc ccgcttccgc
      121 ggcgatcctc aactgatccc aaagcgcctt ttgtgaggcg atgatcgcgt cgtcctcaac
      181 cagcactact ttctcgacgt atttctgagc aagcgggaac atcagctcgc ccacgcgctt
      241 gggcgcgagc gagtcggcgg cgataccgcc ggcgggcgca tcgaccggcc tgcccgcctc
      301 gagcgccctc gcgagcgtcg gcgcgagaac gggctcgacg ccgatgatcc gcaccttgcc
      361 ggcataccag gcggcgatgc cgccaatcag gccgccgccg cccacggcga ccagcagcgt
      421 atccagggat tcttgctcct cgaattccat gccgactgtc ccctggccga gcagcgcgcc
      481 ctcctggtca taggcatgga tctgcatggc gccgctgtcg gcggcccatt tctcggaggc
      541 agcgagcgca tcggcgtagc gctcgccggt gaccaccagt tccgctccgt aggaccggat
      601 gcgctcgatc ttcgcaggcg acgcaaccgt cgggacgtag atgcgcgccg gcttgcccag
      661 cttgtgcgcg gcataagcca ccgcggcgcc atggtttccg ccggacgccg ccaccacacc
      721 cgctgccggc acttcgcgtg tcagcaggtt ggcaaacgcg ccgcgcgcct tgaacgaccc
      781 cgagtgctgc agcagctcga gcttgaatac gcgccggccg tcgccgtccg gatcgatcac
      841 cggcgtgcga cgcacgtacc tcgcgatcaa ctgggccgtt tcggcgatgc gctctcgggt
      901 gaccatgccg tgctagtata tagccaactg gctaattgcc tatggcccgc gtagcccctc
      961 tcgccgcggc gctgcgcgcg ctcgccaacg agcgccgcct gcagattctc gagtggctca
     1021 ggcagccgcg gcgccatttc ccgccccagg tcgacggcga cctggtggag gacggcgtct
     1081 gcacgctgct catcgccgag aagctcggcg tgagccagcc gaccgtcagc gagcacctgc
     1141 gggtgctcgc cgcggcgggg ctgatccgcg gcaagccgat caaacagtgg attttctaca
     1201 agcgcgacga ggcggcgatc ggcgagatca agaaggtgat tcaggcgaaa atctgaccat
     1261 gcccaagcct ctggtttcca ccgacgagct cgccgcgcac cttgcgcaat ggcgcgtgtt
     1321 cgactgccgc cacgacctga tgaagcccga cctcggcgcg cagcaatacc gcgaggcaca
     1381 catccccggt gcgctgttcg ccagcctcga ccgcgacctg tcggcgccga agaccggcaa
     1441 gaatggccgg catcccctgc ccgaccagga cagcttcgta gcctggctcg gccgccaagg
     1501 cgtgcgcccg gaggaccagg tcgtctgcta cgacgcggcc aacggctcca cggcggcacg
     1561 cttgtggtgg atgctgcgct gggtcggcca ccgctcggtg gcggtgctcg acggcggcct
     1621 cgccaagtgg ctgaaggaag gccggccgct cgacgccgtg gttccgcaac cgaagccttc
     1681 gacgtatgca tcaaaaagga ctgccggcac gctcgacgcg ggcgcggtgg cggcaaacct
     1741 gaagaatctt ttcctgctgg atgcgcgcgc gccggcgcgc tatcgcggcg agcaggaacc
     1801 gatcgatccg gtcgccggcc acattccggg cgcgaagaac cgcttcaaca tggacaatgt
     1861 cctgcctgac ggcaccttca agcagagcga cgccttgaag agggattttc ttgccgtgtt
     1921 gaaaaatcaa aaacctgaag acgtcgtgca ctactgcggc tcgggtgtcg cggcctgcca
     1981 caaccttctc gcaatggaga tcgccggcct gccgggcggc aagctgtatc ccggctcttg
     2041 gagcgagtgg ctcgccgacc cagcgagacc gcgaggaaaa gcgtaatgcc ccagcacatc
     2101 ggcatcgtcg cctgctcggc cgaaggcgcg gcgctgtgct acaagacgat ctgcacggag
     2161 ggcgcggagc tgctcggcgc gcacgcgcac ccggaagtct cgatgcatac gccctcgctc
     2221 gccgactaca tggggctcat ctaccgcggc gactggcagg gcgtgggcga cctgatgctc
     2281 gagtcggcgc agaagctctc ggcaatcggc gccgatttcc tgatctgccc ggacaacacc
     2341 atccaccagg ggctgccgta catcgagcgc cgctcgccgc tgccgtggct gcacatcgca
     2401 gagagcgtcg ccgcgcaggc tgccgagcgc ggcttccggc gcctcgggct gaccggcacc
     2461 cgctggctcg tcgacagcgc cgtctacccc gagaagctcc aggcccgcgg catggtctgc
     2521 gtacgcccga gcgacgccga gcgcgacgag atcaaccgga tcatcatgga cgagctggtg
     2581 tacggcgttt tcaagcccga cgcgatcgcc tgcttccagc gcgtcatgcg acagatgaag
     2641 gcccagggct gcgacgccgt catcctcggc tgcaccgaga taccactggt catgagcgac
     2701 gcgaactcgc cgctgccgac gctcgactcg acgcggctgc tcgcgcgcgc ggcgctgcgc
     2761 cgcgcgctgc gctaaaagcc gaacagcttc gccgggttgt cgaccaggat gcggcggcgc
     2821 gtcgcttcgt cccgcgtcca gcccctaaac agctcgaaca gctgcatcgg gttcactttg
     2881 tcggcaaagg acaggtgcgg atagtcgctg ccccagatca ggcggtccgg cgccgcctcg
     2941 atcagtgcgc gcgccagcgg cgcggcgtcg gcgaaccccg gcgcctggga cacgcggtag
     3001 acgcccgtca gcttgaccca gaccttgccg cggccgttac gcagcaggtc gagcagccgc
     3061 tggaagcccg cctgctgcac gccgtccctg gcgaggaaca tccccatgtg ggcgagcgtg
     3121 cagtcgcact gcagcgagcc gatcttcctc atcagctcgt cggtcagcca gccgggcagc
     3181 aggaagtcga ggtgccagcc gattccggcc aggcgcgcgt ccagcttctc gatgcgggcc
     3241 agcagcttca gcgccaggcc cgcctcgggc ggatggacgg gcgacacgtt gatgcgcacg
     3301 ccgcgcacgc ccagcgcgtg catcttcgcc agctcctggt cggacgcatc ctcgtcgaca
     3361 tccacgacgc cgcggcttcc cggcatctcg cgcatcgcgt cgagcatgca gctgttgtcc
     3421 cgcagataga cgctcggctg cacgaagacg aagcgctcga agcccaaggc gcgcgcgtgc
     3481 accaggtagt cctcgagcgg cgcgagcggc ggcttgtagc gcagcttcgg atcgcgagcc
     3541 ggatacttgt cggccgg
//
LOCUS       DASYJA010000342         2375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1957140, whole genome shotgun sequence.
ACCESSION   DASYJA010000342 DASYJA010000000
VERSION     DASYJA010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1957140"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VGO02_07515"
     CDS             <1..241
                     /locus_tag="VGO02_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477569.1"
                     /translation="TRPVGYKMAYGTDKHEDYFVEAAERARGGKMLREELFHLREELE
                     AMEARGALPAKLRDLKENLVRLRMETAGEEGKAEA"
     gene            242..1423
                     /locus_tag="VGO02_07520"
     CDS             242..1423
                     /locus_tag="VGO02_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEV7477570.1"
                     /translation="MEAILLGIYAFFVWLIFIKLKWLPWNLYSQVTCAIIPVIGLAAL
                     ILLLNVFAPSSSDVRVFKYTVPIVSQVRGRVVEVPVIEGNVLVKKGEVLFKIDPEPYQ
                     LAVNQLEAQLASAVAGQRELREALKGAKGAIDQAAARAQETSARLDLARTRVVQNREL
                     SRTGAGNKFELEQAEANQKELQGQYDAARAAQVQAVAGERQIFEKLGANVKGEFAQVA
                     QIRAQLETARWELEQTTTVSPCDCYVINLQLRPGAFVAGFPINPVMTLVEATGQVIAL
                     YNQNELHQVQPGNEAEFTLITDPGRVVKGKVDSIVWAQGAGQLQAGGTLPMTGVLHAP
                     PNRFAVKFDIADKDKDLFMAAGAAGSAAIYTDHLHAVHVIRKVILRVGAYTNYLVLKL
                     H"
     gene            1425..>2375
                     /locus_tag="VGO02_07525"
     CDS             1425..>2375
                     /locus_tag="VGO02_07525"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEV7477571.1"
                     /translation="MRAARRLVAPILLAAAAGCALKSPPAPEETRKQALPNLAVPEVW
                     SAEAATGAAAAGWLASFNDPQLEAMVREALANNPDLGVAAARVEQAAGFVKASGATLY
                     PQVSLMARGGGRMSGDSSGLEGAGVFATWELDLWGRVRAERQTARLQYDSTALDAEYA
                     RQSIAALVAKSWFLATEARMQRALADEMVTSSVRLVSLAGDRLRVGNGNEYDLALARA
                     NVQTFQDAQHQLDAAYRQALRALEALAGRYPAAALQVPARLVERPAPVPAGIPSELLE
                     RRPDVAAAERRVAAAFYRIETAKAARLPSISLTASGSSISS"
BASE COUNT          365 a          821 c          873 g          316 t
ORIGIN      
        1 cacgcgcccg gtcggctaca agatggctta tggcaccgac aagcacgagg actacttcgt
       61 cgaggcggcg gagcgggcgc gcggcggcaa gatgctgcgc gaggagctgt tccacctgcg
      121 cgaggagctc gaggcgatgg aggcgcgcgg cgcgctgccg gcgaagctgc gcgacctcaa
      181 ggaaaatctg gtgcgcctgc gcatggagac cgccggcgaa gagggcaagg cagaggcgta
      241 gatggaagcc attctcctcg gtatctacgc gttcttcgtc tggctgatct tcatcaagct
      301 gaagtggctg ccgtggaacc tctacagcca ggtgacctgc gccatcatcc cggtcatcgg
      361 cctggcggcg ctgatcctgc tgctcaacgt gttcgcgccg tcctcgtccg acgtgcgcgt
      421 cttcaagtac accgtgccga tcgtgtcgca ggtgaggggg cgggtggtcg aggtgccggt
      481 gatcgagggc aacgtgctgg tgaagaaggg cgaggtgctc ttcaagatcg acccggagcc
      541 ctaccagctc gccgtcaacc agctcgaggc gcagctcgcc agcgccgtgg ccggccagcg
      601 cgagctgcgc gaggcgctga agggcgcgaa aggcgcgatc gaccaggcgg cggcgcgcgc
      661 gcaggagacc agcgcccggc tcgacctcgc gcgcacgcgc gtggtgcaga accgcgagct
      721 gtcgcgcacc ggcgccggca acaagttcga gctggagcag gccgaggcca accagaagga
      781 gctgcaggga cagtacgacg cggcgcgcgc cgcgcaggtg caggcggtcg ccggcgagcg
      841 gcagatcttc gagaagctgg gcgccaacgt gaagggcgag ttcgcgcagg tggcgcagat
      901 ccgcgcgcag ctcgagaccg cccgctggga gctcgagcag acgacgaccg tgtcgccgtg
      961 cgattgctac gtcatcaacc tgcagctgcg gcccggcgcg ttcgtcgccg gcttcccgat
     1021 caacccggtg atgacgctgg tcgaggcgac gggccaggtg atcgcgctct acaaccagaa
     1081 cgagctgcac caggtgcagc cgggcaacga ggccgagttc acgctgatca ccgacccggg
     1141 tcgcgtcgtc aagggcaagg tcgattccat cgtctgggcg cagggggcgg gccagctgca
     1201 ggccggcggc acgctgccga tgacgggcgt tctgcacgcg ccgcccaacc gcttcgcggt
     1261 caagttcgac atcgccgaca aggacaagga cctgttcatg gcggcgggcg ccgccggcag
     1321 cgccgccatc tacaccgacc acctgcatgc cgtgcacgtc atccgcaagg tgatcctgcg
     1381 cgtcggcgcg tacaccaact atctggtcct gaagctgcac tgagatgcgc gcagcgcgtc
     1441 gactggtcgc cccgatcctg ctcgccgcgg cggccggctg cgcgctgaag tcgccgcccg
     1501 cgccggagga gacgcgcaag caggcgctgc ccaacctcgc ggtgccggag gtctggtcgg
     1561 ccgaggcggc taccggcgcg gcggccgccg gctggctggc gagcttcaac gacccccagc
     1621 tcgaggcgat ggtgcgcgag gcgctcgcca acaatccgga cctcggcgtg gcggcggcgc
     1681 gcgtcgagca ggcggccggc ttcgtcaagg cgtccggcgc cacgctctac ccgcaggtca
     1741 gcctcatggc gcgcggtggc ggccgcatga gcggcgactc gagcgggctg gagggcgccg
     1801 gcgtgttcgc cacctgggag ctcgacctct gggggcgggt gcgcgccgag cggcagaccg
     1861 cgcggctgca gtacgactcg accgcgctcg acgccgagta cgcgcgccag tcgatcgccg
     1921 cgctggtcgc caagagctgg ttcctcgcca ccgaggcccg catgcagaga gcgctggccg
     1981 acgagatggt gacctcgtcg gtgcggctgg tatcgctcgc cggcgaccgc ctgcgcgtcg
     2041 gcaacggcaa cgagtacgac ctggcgcttg cccgcgccaa cgtgcagacc tttcaggacg
     2101 cgcagcacca gctcgacgcg gcgtaccgcc aggccctgcg cgccctcgag gcgctcgccg
     2161 ggcgctatcc ggcggctgcg ctgcaggtgc cggcgcgact cgtcgagcgg ccggcgccgg
     2221 tgcccgccgg catcccctcg gagctgctcg agcggcgccc cgacgtggcg gcggccgagc
     2281 gccgggtggc ggcggcgttc taccgcatcg agaccgccaa ggcggcgcgc ctgccgagca
     2341 tttcgctcac cgccagcggc tcgagcatct cgagc
//
LOCUS       DASYJA010000343         2485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_50516, whole genome shotgun sequence.
ACCESSION   DASYJA010000343 DASYJA010000000
VERSION     DASYJA010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50516"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="VGO02_07530"
     CDS             complement(<1..178)
                     /locus_tag="VGO02_07530"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV7477572.1"
                     /translation="MSHASPGAPPAVPPMVLTFAASDPTGGAGVQADLLTIAALGCHP
                     LSVVTALTVQDTHGV"
     gene            217..387
                     /locus_tag="VGO02_07535"
     CDS             217..387
                     /locus_tag="VGO02_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEV7477573.1"
                     /translation="MQYKTWMCLICGFVYDEAAGLPDEGLAPGTRWEDVPMNWTCPEC
                     GARKEDFEMVEI"
     gene            414..974
                     /locus_tag="VGO02_07540"
     CDS             414..974
                     /locus_tag="VGO02_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEV7477574.1"
                     /translation="MSSINLTHHFLIAMPSMADPYFSKTLTYICEHNDQGALGLVVNR
                     PIDMTLQALFERLSLSLRDAGLVHAPIYFGGPVQTDRGFVLHEPAGNWQSTLRVRDAI
                     GLTTSKDILEAVGRGEGPKRMMVTLGYAGWSPGQLEHELSQNAWLTVEARDGIIFETP
                     AEERLPAAMELLGIDFATLQDAAGHA"
     gene            967..1377
                     /gene="ruvX"
                     /locus_tag="VGO02_07545"
     CDS             967..1377
                     /gene="ruvX"
                     /locus_tag="VGO02_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV7477575.1"
                     /translation="MPEQAPAGKHPVRTVLAFDFGLKRIGVAVGEPELRTAHPLSAVA
                     HFSQIEKLVEEWKPASLVVGLPTSVQGVAHAMTKQAEDFARRLEKRFKLPVARVDERY
                     TSVEAESRLKGLKKKAIDSVAAQLILEQYFDEAA"
     gene            1364..1864
                     /gene="pyrR"
                     /locus_tag="VGO02_07550"
     CDS             1364..1864
                     /gene="pyrR"
                     /locus_tag="VGO02_07550"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEV7477576.1"
                     /translation="MKLPDVEQLCEQLAAELRPRIGPKSAMIGIHTGGAWVAERLHAL
                     LKLPEPLGLMDIAFYRDDYHAQGLHHDPKRTKIPFDVNGRELLLVDDVLYSGRTVRAA
                     MNELFDYGRPASISLAVLVDRGGRQLPICAQHCGARLDVPAGMRLRLKRGADGKLALA
                     LESHGE"
     gene            1863..2485
                     /gene="pyrB"
                     /locus_tag="VGO02_07555"
                     /pseudo
     CDS             1863..2485
                     /gene="pyrB"
                     /locus_tag="VGO02_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600263.1"
                     /note="catalyzes the transfer of the carbamoyl moiety from
                     carbamoyl phosphate to L- aspartate in pyrimidine
                     biosynthesis; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
BASE COUNT          439 a          843 c          820 g          383 t
ORIGIN      
        1 cgacgccgtg cgtatcctgc acggtgagtg cggtgacgac cgagagcgga tgacagccga
       61 gcgcggcgat ggtgaggaga tccgcttgca cgccggcgcc gccagtcgga tcggatgcgg
      121 cgaaggtcaa caccataggc ggaacggcgg gcggcgctcc gggcgaagcg tgcgacatgg
      181 gttaatatcc gggcccattc tatggccgaa aatcaagtgc aatacaagac gtggatgtgc
      241 ctcatctgcg gcttcgtgta cgacgaggcg gccgggctgc ccgacgaagg cctcgcgccg
      301 ggcacgcgct gggaggacgt gccgatgaac tggacctgcc ccgagtgcgg cgcgcgcaag
      361 gaagacttcg aaatggtgga gatctgatgc ggtaaaatgc gctggaaggg cgcatgtcct
      421 cgatcaatct gacgcatcac ttcttgatcg ccatgcccag catggccgat ccgtactttt
      481 cgaagaccct gacgtacatc tgcgagcaca acgaccaggg cgcgctcggc ctggtcgtca
      541 accgcccgat cgacatgacc ctgcaggccc tgttcgagcg cctgtcgctt tcgctgcgcg
      601 acgcaggcct ggtgcatgcg ccgatctact tcggcgggcc ggtgcagacc gaccgcggct
      661 tcgtgctgca cgagccggcg ggcaactggc aatcgacgct gcgcgtgcgc gacgccatcg
      721 gcctcaccac ctcgaaggac atcctcgagg cggtcggccg cggcgagggg cccaagcgca
      781 tgatggtcac cctgggctac gccggctggt cgccggggca gctcgagcac gagctgtcgc
      841 agaacgcctg gctcacggtc gaggcgcgcg acggcatcat cttcgagacc ccggcggagg
      901 agcgcctgcc ggcggcgatg gagctcctcg gcatcgattt cgcgacgctg caggacgcgg
      961 cgggacatgc ctgagcaggc gccagccggc aaacaccctg ttcgtactgt tctagccttc
     1021 gatttcggtc tcaagcgcat cggcgtcgcc gtcggcgagc cggaactgcg cacggcgcat
     1081 ccgctttccg cggtcgcgca tttttctcaa atagagaaac tcgtagaaga atggaaaccc
     1141 gccagcttgg tggtcggtct gccgacctcg gtgcagggag tcgcgcacgc catgacgaag
     1201 caggccgagg atttcgcccg ccggctcgag aagcgcttca agctgccggt tgcccgggtc
     1261 gacgagcgct atacctcggt cgaggcggag tcccggctga aaggcctcaa gaagaaagcg
     1321 atcgactcgg tggctgcgca actgatcctg gaacagtact tcgatgaagc tgcctgacgt
     1381 cgaacaactc tgcgagcagc tcgccgccga gctcaggccg cgcatcgggc cgaagagcgc
     1441 gatgatcggc atccataccg gcggcgcctg ggtcgccgag cggctgcacg cgctgctcaa
     1501 gctgcccgag ccgcttggcc tgatggacat tgcgttctac cgcgacgact accacgcgca
     1561 gggcctgcac cacgatccga agcgcaccaa gatcccgttc gacgtcaacg gccgcgagct
     1621 gctgctggtc gacgatgttc tctacagcgg gcgcacggtg cgtgcggcga tgaacgagct
     1681 cttcgactac ggcaggccgg cgagcatctc gctggcggtc ctcgtcgacc gcggcggccg
     1741 ccagctgccc atttgcgcgc agcattgcgg cgcgcgcctg gacgtgcccg ccggcatgcg
     1801 cctgcgcctg aagcgcggcg ccgacggcaa gctcgcgctc gcgctggaga gccacggtga
     1861 ataacccaca gctcaacgcc aacggcgagc tgcagcacct gctctccacc gagggcctgc
     1921 cgcgcgaaat cctgacgcag atcctcgaca ccgccgaatc cttcgccggc gtcacggagc
     1981 gcgaggtgaa gaaagtgccg ctgctgcgcg gcaagagcgt gttcaacctg ttcttcgagc
     2041 cctcgacgcg cacccgcacg accttcgaga tcgcggcgaa gcgcctgtcg gccgacgtca
     2101 tcaacctgaa catcgccatc tcgtcgcaga ccaagggcga atcgctgctc gacacggtgg
     2161 ccaacctctc ggcgatgcag gccgacatct tcgtcgtgcg ccacgcctcg gccggcgcgc
     2221 cgttcctcat cgcgcgtcac gtcaagccgc acgagcacgt ggtgaacgcg ggcgacggcc
     2281 ggcacgccca cccgacgcaa ggactgctcg acctgttcac catccgccac tacaagaagg
     2341 acttcagcaa gctcacggtg gcgatcgtcg gcgacatcct gcactcgcgc gtggcgcgct
     2401 cgctgatcca cggcctcacc acgctcggcg cgccggaggt gcgcgtcatc gggccgcaga
     2461 cgttgctccc ggccggggtc gaggc
//
LOCUS       DASYJA010000344         3411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2320187, whole genome shotgun sequence.
ACCESSION   DASYJA010000344 DASYJA010000000
VERSION     DASYJA010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2320187"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="VGO02_07560"
     CDS             <1..859
                     /locus_tag="VGO02_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7477577.1"
                     /translation="LGVAAAGRGCVVGTIPPQHVYGFESTVMLPLQSGAALHSGRPLL
                     PADIAAALQETSAPRWLATTPVQLRACVGEAAALPQLAGVLSATMPLDAPLAAQAERL
                     WSAPVHELYGCTEIGGIALRRAAGGAAWRTLQGMQLEQRGEETWARGAQLAAPARLPD
                     RIALLSPTEFTLLGRPEDLVKIAGKRASLEDLNRELLAVRGVRDGAFFVPEDDGGAQA
                     ARLAALAVAPGCAAREILDALRGRIDAAFLPRPLLIVEALPRNATGKLPREALLALAR
                     SAAHGRRSA"
     gene            856..1143
                     /locus_tag="VGO02_07565"
     CDS             856..1143
                     /locus_tag="VGO02_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase"
                     /protein_id="HEV7477578.1"
                     /translation="MIRIADEVGPAHPALAGHFPGNPIVPGALLLARVARAAAAAFGA
                     GVAGAPAVKFLAPLRPGERFEVELEAAKALVKFRVLRGATLIAAGTLKLAQ"
     gene            1197..2036
                     /locus_tag="VGO02_07570"
     CDS             1197..2036
                     /locus_tag="VGO02_07570"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEV7477579.1"
                     /translation="MARLSLGLGRGLTRCLLPPLCLYYYATAPRARRASREFLARALG
                     REPRLRDALRHYHAFASTMHDRLLLAAGHHAALDIAVSGAEQIDRLLAAGRGCLLVGS
                     HLGSFEVLRALERTSVHRVSVVMHEENARRMQALISRLAPGLQQRVIASGRPDTMLRI
                     KERLERGGIVGILGDRPLSEERTASRDFLGAPARFPLGPWLLAATLGCPVGLFFGLYR
                     GGARYEIELETFSEGERVPRQERDAAAASALARFVARLEHHARRAPDNWFNFYDFWEA
                     GRA"
     gene            2038..2586
                     /locus_tag="VGO02_07575"
     CDS             2038..2586
                     /locus_tag="VGO02_07575"
                     /inference="COORDINATES: protein motif:HMM:NF039988.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-related protein"
                     /protein_id="HEV7477580.1"
                     /translation="MALVCAARICAAQALAPADLMRQLAGVESSRATFVETRHSALLK
                     EPLVLRGTLAWQRPASLEKRVTQPYDERIALDGDVLTIENRRGRKLKTSVSGSPAIAA
                     LVEGIRASRAGDLAALERHFAVRVDGGAGRWTLSLKPIDEEVARQVASIAISGAQGRI
                     SRVEVQETGGDRSVMEIREEIR"
     gene            2583..>3411
                     /locus_tag="VGO02_07580"
     CDS             2583..>3411
                     /locus_tag="VGO02_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV7477581.1"
                     /translation="MKPARLAVIGLWLLALAACAVWLHGHLSVQSEMTVFLPPSATPA
                     QRLLLSQLREGTASRLILIELRGAAAPELARASGELARRLRESGLFSSVNNGDVAALA
                     AERQLLLQHRYLLSPAVNAERFSSAGLAAALQESLELLASPAGALLRASIPVDPTGEL
                     RELARLVLPGDGPPRRHGVWFSRDGAAALLVAETRAAGFDVDAQARAAAAVREAFGAA
                     LAGRGSLAMASPGVFAVQMRETIEAESWRLSLAAALLVLLILVLAYRCAAAVAVCAVP
                     "
BASE COUNT          384 a         1284 c         1263 g          480 t
ORIGIN      
        1 cctgggcgtc gcggcggccg ggcgcggctg cgttgtcggc acgattccgc cgcagcacgt
       61 ctacggcttc gagagcacgg tgatgctgcc gctgcagagc ggcgccgcgc tgcattccgg
      121 ccggccgctg ctgccggccg acatcgccgc ggcgctgcag gagacttcgg cgccgcgatg
      181 gctcgcgacg acgccggtgc agctgcgcgc ctgcgtcggc gaggcagcgg cgctgccgca
      241 gcttgccggc gtgctcagcg ccaccatgcc gctcgacgcg ccgctcgccg cgcaggccga
      301 gcggctctgg tcggcgccgg tgcacgagct ctacggctgc accgagatcg gcggcatcgc
      361 cttgcggcgc gcggcgggcg gcgcagcatg gcgcacgttg cagggcatgc agctggaaca
      421 gcgcggcgag gaaacctggg cccggggcgc ccagctcgct gcgccggcgc gcctgcccga
      481 ccgcatcgct ctcttgagcc cgaccgagtt cacgctgctc ggccggcccg aggacctggt
      541 caagatcgcc ggcaagcgcg cctcgctcga ggacctcaac cgcgagctgc tggcggtgcg
      601 cggcgtgcgc gacggcgcct ttttcgtgcc ggaggacgac ggcggcgccc aggccgcgcg
      661 gctggccgcg ttggcggtcg cgccgggctg cgccgcgcgc gagatcctcg acgcgctgcg
      721 cgggcgcatc gatgccgcgt tcctgccgcg gccgctgctc atcgtggaag cgctgccgcg
      781 caacgccacc ggcaagctgc cgcgcgaggc gctgctcgcg ctggcgcgct cggcggcgca
      841 cggccggcgc agcgcatgat ccgcatagcc gacgaggtcg gccccgcgca tccagccctc
      901 gccggccatt tcccggggaa tccgatagtg cccggagcgc tgctgctcgc gcgcgtcgcg
      961 cgcgctgccg cggctgcctt cggcgccggt gtcgccggcg cgccggcagt gaagttcctc
     1021 gctcctctgc ggcccggcga gcgcttcgag gtcgagctcg aggcggcaaa ggcgctggtg
     1081 aaattccgcg tcttgcgcgg cgcgacgctg atcgccgccg gaaccttgaa gctggcccag
     1141 tgagcacggc gtggcgcgaa gcagccgagc gcggcacagc gagtggcctg acgctgatgg
     1201 cccgcctcag cctcggcctg gggcgcggcc tcacccgctg cctgctgcct ccgctgtgcc
     1261 tctactacta cgcgacggcg ccgcgggcgc gccgcgcctc gcgcgagttt ctcgcccgag
     1321 cgctcggccg cgagccacgc ctgcgggacg cgctgcgcca ctaccacgct ttcgcctcga
     1381 ccatgcacga ccgtctgctg ctcgccgccg gacaccacgc ggcgctcgac atcgcggtgt
     1441 cgggagccga gcagatcgac cgcttgctcg ccgccggccg cggctgcctg ctggtgggct
     1501 cgcacctggg cagcttcgaa gtgctgcgcg cgctggagcg cacgtcggtc catcgggtaa
     1561 gcgtggtgat gcacgaggag aacgcgaggc ggatgcaggc gttgatctcg cgcctggcgc
     1621 cgggattgca gcagcgggtc atcgcttccg ggcggcccga caccatgctg cgcatcaagg
     1681 agcgcctgga gcgcggcggg atcgtcggca tcctcggcga ccgtccgctg agcgaggagc
     1741 gcaccgcgag ccgcgacttc ctcggcgccc cggcgcgctt tccgctgggg ccgtggctgc
     1801 tcgcggcgac gctcggctgc ccggtcggct tgttcttcgg cctgtaccgc ggcggcgcgc
     1861 gctacgagat cgagctcgag acgttcagcg agggcgagcg cgtcccgcgg caggagcgcg
     1921 acgccgcggc ggcgagcgcc ttggcgcgct tcgtcgcgcg cctcgagcac cacgcgcgcc
     1981 gcgcgcccga caactggttc aacttctatg atttctggga agcgggccgc gcttagcgtg
     2041 gcactcgtct gtgcggcgcg gatctgcgcc gcgcaggcgc tcgccccggc cgacctgatg
     2101 cgccagctcg ccggcgtcga gagctcgcgc gccaccttcg tcgagacgcg gcacagcgcg
     2161 ctgctgaagg agccgctggt cctgcgcggc acgctcgcct ggcagcgtcc cgcctccctc
     2221 gagaagcgcg tcacgcagcc ctacgacgag cgcatcgcgc tcgacggcga cgtcctcacg
     2281 atcgagaacc ggcgcggcag gaagctgaag acctcggtgt cgggctcgcc ggcgatcgcg
     2341 gcgctggtgg agggcatccg cgccagccgc gccggcgatc tcgcggccct cgagcggcac
     2401 ttcgcggtgc gcgtcgacgg cggcgcgggt cgatggaccc tgtcgctcaa gccgatcgac
     2461 gaggaggtgg cgcgccaggt cgcctcgatc gcgatctccg gcgcgcaggg ccgcatctcg
     2521 cgcgtggagg tgcaggagac cggcggcgat cgctcggtga tggaaatccg cgaagagatc
     2581 cggtgaagcc ggcccggctg gccgtgatcg gcttgtggct gcttgcgctc gccgcctgcg
     2641 ccgtctggct gcacggccac ctctcggtgc agagcgagat gacggtgttc ctgccgcctt
     2701 cggcgacccc ggcgcagcgc ctgctcctga gccagctgcg cgagggcacg gcgtcgcgcc
     2761 tgatcctgat cgagctgcgc ggcgcggccg cgcccgagct cgcgcgcgcg agcggcgagc
     2821 ttgcgcggcg cctgcgcgaa agcggcctgt tctcttcggt caacaacggc gatgtcgccg
     2881 cgctcgccgc ggagcgccag ctcctccttc agcaccgcta cctgctcagc ccggcggtaa
     2941 acgccgagcg cttctccagc gccggcctcg ccgccgcgct gcaggagagc ctcgagctac
     3001 tcgcttcgcc cgccggcgcc ctgctgcgag cgtcgatccc ggtcgatccg acgggcgagc
     3061 tgcgcgagct ggcccggctt gtgctgcccg gcgacgggcc gccgcggcgc cacggcgtct
     3121 ggttcagccg cgacggcgca gccgcccttc tcgtcgccga gacgcgcgcc gccggattcg
     3181 acgtcgacgc gcaggcccgc gccgcagccg ccgtgcgcga ggcgttcggc gccgcgctgg
     3241 cgggacgcgg cagcctcgcg atggccagtc cgggcgtgtt cgccgtgcag atgcgcgaga
     3301 cgatcgaggc cgagtcctgg cgcctgtccc tcgccgccgc gctcctggtc cttctcatcc
     3361 tggtgctcgc ctatcgctgc gcggcggcgg tcgccgtatg cgccgtgccg g
//
LOCUS       DASYJA010000345         4959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2683648, whole genome shotgun sequence.
ACCESSION   DASYJA010000345 DASYJA010000000
VERSION     DASYJA010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2683648"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1523)
                     /gene="alaS"
                     /locus_tag="VGO02_07585"
     CDS             complement(<1..1523)
                     /gene="alaS"
                     /locus_tag="VGO02_07585"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEV7477582.1"
                     /translation="MKSNEIRSGFLEYFRSKGHAIVPSSPLVPQNDPTLLFVNSGMVQ
                     FKDVFLGKDKRPYTRATTSQRSVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     KDAIQFAWELLTKVYKLPAERLWITVYQTDDEAYDLWTKLIGVPKERVVRIGDKPGGP
                     KYQSDNFWQMADTGPCGPCSEIFYDHGAGIAGGPPGSPDADGDRYIEIWNLVFMQFNR
                     DEKGVLHPLPKPSVDTGMGLERIAAVLQHVHSNYEIDLFQDLIKAAARETGTKDLKNP
                     SLNVIADHIRACSFLVVDGVIPGNEGRGYVLRRIVRRAIRHGYKLGQKQPFFHRLVPD
                     LDKAMGDAYPELRAAKQKVADVLKQEEERFAETLENGMGVLESALASGEKLLDGETVF
                     RLYDTFGFPVDLTADICRERGVMIDMAGYEAAMAQQRERARAASKFSMAAGVEYSGGK
                     TVFHGYEKLAHPGKVVALYREGSQVQSLRSGEAGTVVLNETPFYAESGGQVGDRGELV
                     "
     gene            complement(1596..1934)
                     /locus_tag="VGO02_07590"
     CDS             complement(1596..1934)
                     /locus_tag="VGO02_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477583.1"
                     /translation="MDQAQALALVRSLANGVDPESGEIFPAESAYQRPQIVRALYEAA
                     SALERIERFERRRAQMPAKTGEAWSEEEDRKLLAAFDAGRALQELAAAHERTMGAVRA
                     RLLKYGRINA"
     gene            complement(2032..2472)
                     /gene="recX"
                     /locus_tag="VGO02_07595"
     CDS             complement(2032..2472)
                     /gene="recX"
                     /locus_tag="VGO02_07595"
                     /inference="COORDINATES: protein motif:HMM:NF001055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEV7477584.1"
                     /translation="MKEKPDTPAQLKTRALRFLVRREHSRAELVGKLAPYAESEQALN
                     AVVDLLQSKKQLSDERFAEERARSLSRKYGAAMIRQDLKERGIAEEIVGRISAEGDLD
                     RAKAILSRKYRAAATTREEHAKRARFLQSRGFSYEVIRSVLKPE"
     gene            complement(2469..3551)
                     /gene="recA"
                     /locus_tag="VGO02_07600"
     CDS             complement(2469..3551)
                     /gene="recA"
                     /locus_tag="VGO02_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV7477585.1"
                     /translation="MDDSKSKALAAALSQIEKQYGKGSIMKMDDAAIRDIDVVSTGSL
                     GLDLALGVGGLPRGRVVEIYGPESSGKTTLTLQVIAEMQKVGGTAAFIDAENALDISY
                     AAKLGVKTEDLLISQPDTGEQALEIADMLVRSGAVDVIVIDSVAALTPKAEIEGEMGE
                     PQMGLQARLMSQALRKLTGNIKRTNTLVIFINQIRMKIGVMFGNPETTTGGNALKFYA
                     SVRIDIRRIGSIKKGDEVTGSETRVKVVKNKVAPPFRQAEFDILYGEGISREGEMLEL
                     GVNHGVLEKSGAWYIYNGDRLGQGKDNSRDFLKENPALAKEIEGKIREKAGVKAATPA
                     AAAAAVEEPEDKKVERLPTRKRSNEG"
     gene            3656..4579
                     /locus_tag="VGO02_07605"
     CDS             3656..4579
                     /locus_tag="VGO02_07605"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7477586.1"
                     /translation="MTATCQLPENSLGDKKMAKVAFLGLGVMGYPMAGHLLKKGGHEV
                     TVYNRSAAKAQQWVKEYGGRSAATPREAAKDCDFVMLCVGNDDDVRSVVYGDSGALAG
                     MKKGAILVDHTTASAVLARELFEKSKSVSVGFIDAPVSGGQAGAVNGQLGIMCGGEEA
                     TFAKAKPVIDVYAKLAALIGGPGAGQLTKMVNQICIAGLVQGLAEGLAFAKRSNLDVE
                     KVVGVISKGAAQSWQMENRWKQMNEVKFDGFGFAVDWMRKDLGICIEESKKNGARLPV
                     TALVDQFYAHIQARGGKRWDTSSLMHLLMKD"
     gene            complement(4576..>4959)
                     /locus_tag="VGO02_07610"
     CDS             complement(4576..>4959)
                     /locus_tag="VGO02_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEV7477587.1"
                     /translation="GLALVLAACAPRREAPPPSGIDAYKRIVALDVLQHAPGTYCQPG
                     EEMLKSVVILEIAIDAEGRLLGAAVVRSNGYPELDQRALDTVQSAAPFSAPDADLVNG
                     AASVKFLETFLFREDDCFHIRSLAR"
BASE COUNT          808 a         1620 c         1607 g          924 t
ORIGIN      
        1 accagctcgc cgcgatcgcc cacctggccg cctgactcgg cgtagaacgg cgtctcgttc
       61 agcaccaccg tgcccgcttc gccgctcctc aggctttgca cctggctgcc ttcgcgatag
      121 agcgcgacca ccttgcccgg atgcgcgagc ttctcgtagc cgtggaaaac ggtcttgcca
      181 cccgagtatt cgacccctgc ggccatcgag aacttcgatg cggcgcgcgc gcgctcgcgc
      241 tgctgcgcca tcgccgcctc gtagcccgcc atgtcgatca tcacgccgcg ctcgcggcag
      301 atgtcggcgg tgaggtcgac ggggaagccg aaagtgtcgt agagccggaa gaccgtctcg
      361 ccgtcgagca gcttctcgcc cgaggcgagc gccgactcga gcacgcccat gccgttctcg
      421 agcgtctccg cgaagcgctc ttcctcctgc ttcaggacgt cggcgacctt ctgcttcgcc
      481 gcgcgcagct ccgggtaggc gtcgcccatc gccttgtcca ggtccggcac cagccggtgg
      541 aagaacggct gcttctggcc gagcttgtag ccgtggcgaa tggcccttct cacaattctt
      601 ctcaagacat acccgcggcc ctcattcccc gggatcacgc cgtcgacgac caggaaggag
      661 caggcgcgga tgtggtcggc gatgacgttg agcgaggggt ttttcagatc cttggtaccg
      721 gtctcgcgag ccgcggcctt gatcaggtcc tggaacagat cgatctcata gttcgagtgc
      781 acgtgctgca gcaccgcggc gatgcgctcc aggcccatgc cggtgtcgac cgagggcttg
      841 ggaagggggt gcagcacgcc cttctcgtcg cggttgaact gcatgaagac caggttccag
      901 atctcgatgt agcggtcgcc gtcggcatcg ggcgagcccg gcgggccgcc ggcgatgccg
      961 gcgccgtggt cgtagaagat ctcgctgcac gggccgcacg ggccggtgtc ggccatctgc
     1021 cagaaattgt ccgactggta cttcgggccg cccggcttgt cgccgatgcg cacgacgcgc
     1081 tccttcggca cgccgatgag cttggtccac aggtcgtagg cctcgtcgtc ggtctggtag
     1141 accgtgatcc acagacgctc cgccggcagc ttgtagacct tggtgagcag ctcccaggcg
     1201 aactggatcg cgtccttctt gaagtagtcg ccgaacgaga agttgccgag catctcgaag
     1261 aacgtgtggt ggcgcgccgt gtagccgacg ttctcgaggt cgttgtgctt gccgccggcg
     1321 cgcaccgagc gctgggaggt ggtggcgcgc gtgtagggtc gcttgtcctt gccgaggaac
     1381 acgtccttga actggaccat gcccgagttg acgaacagca gcgtggggtc gttttgcggc
     1441 accagcgggc tcgagggcac gatggcgtgg cccttggagc ggaagtactc caggaagccg
     1501 gagcggatct cgtttgattt cactacggga ttctactgca gcaggagaaa aagccggggt
     1561 gccgcggaca ccccggaacc actctctcca ggaaactagg cgttgattcg cccgtacttc
     1621 aagagcctcg cgcgcaccgc gcccatcgtg cgctcgtgcg ccgccgccag ctcctgcaac
     1681 gcgcgccctg cgtcgaacgc cgccagcagc ttgcggtcct cctcctcgct ccacgcctcg
     1741 ccggtcttgg ccggcatctg cgcgcggcgc cgctcgaagc gctcgatgcg ctcgagcgcg
     1801 ctcgccgcct cgtacagcgc ccgcacgatc tgcggccgct ggtaggcgct ctccgccggg
     1861 aatatctccc cgctttccgg atcgacgccg ttcgccagcg acctgacgag cgccaatgct
     1921 tgtgcttgat ccattccgtt cacctcccct ccacgcttgt tgcgtgaggt atgaacgctc
     1981 cgtttctcgc ggcggatgac tgccgcgaaa gaaaaatacc ggaagataaa actactcggg
     2041 cttgaggacg ctgcggatca cttcgtagga gaagccgcgg ctctgcagga aacgcgcgcg
     2101 cttcgcgtgc tcctcgcgcg tcgtcgcggc cgcgcggtac ttgcgggaaa gaatggcctt
     2161 tgctctgtcg agatcgcctt cggccgaaat gcgcccgacg atttcttcgg caatcccgcg
     2221 ctccttcaga tcctggcgga tcatcgcggc gccgtacttg cgggaaagag aacgagcgcg
     2281 ttcttcagcg aagcgctcgt ccgaaagctg cttcttcgac tgcaggagat caacaacggc
     2341 attcaatgct tgttccgatt cggcgtacgg cgcgagctta ccgaccagct cggcccgcga
     2401 gtgctcgcgc ctcacgagga atcgcagcgc ccgcgtcttc agctgcgcgg gcgtgtcagg
     2461 cttttctttc aaccttcgtt gctacgtttc cgggtgggaa ggcgttcgac tttcttgtct
     2521 tccggctctt ccacggccgc cgccgcggcg gcaggcgtcg ccgccttcac gccggccttt
     2581 tcgcggatct tgccttcgat ctccttcgcc agcgcggggt tctccttgag gaagtcgcgc
     2641 gagttgtcct tgccctggcc gaggcgatcg ccgttgtaga tgtaccaggc gccggatttc
     2701 tccagcacgc cgtggttcac gccgagctcc agcatctcgc cttcgcgcga gatgccctcg
     2761 ccgtacagga tgtcgaactc cgcctgccgg aaaggcggcg ccaccttgtt cttcaccacc
     2821 ttgacccggg tctcggagcc ggtcacctcg tcgcccttct tgatcgagcc gatgcgccgg
     2881 atgtcgatgc gcaccgaggc atagaacttg agcgcgttgc cgccggtggt ggtctcgggg
     2941 ttgccgaaca tgacgccgat cttcatgcgg atctggttga tgaagatcac cagcgtgttg
     3001 gtgcgcttga tgttgccggt gagcttgcgc agcgcctggc tcatgaggcg cgcctgcaac
     3061 cccatctgcg gctcgcccat ctcgccctcg atctcggcct tgggcgtgag cgccgccacc
     3121 gagtcgatga cgatgacgtc gaccgcgccg gagcgcacca gcatgtcggc gatctcgagc
     3181 gcctgctcgc cggtgtcggg ctgcgagatc agcaggtctt cggtcttgac gccgagcttg
     3241 gcggcatagg aaatgtcgag cgcgttctcc gcatcgatga aggcggcggt gccgccgacc
     3301 ttctgcattt cggcgatgac ctggagggtc agggtcgtct tgcccgagga ctccgggccg
     3361 tagatctcga ccacccggcc gcgcggcagg ccgcccacgc ccagcgccag atccaggccg
     3421 agcgagccgg tggacaccac gtcgatgtcc cggatcgccg cgtcgtccat cttcatgatc
     3481 gagcccttgc cgtactgctt ctcgatctgc gacagggccg ccgccaatgc cttagacttg
     3541 ctgtcatcca tttgctgcac ctctttttct aggttggcat ctacggcagc cgcattttca
     3601 acgaggcggt gggccaacac cgggtactgt ataaacgtcc agctatcgta ggtcgttgac
     3661 cgcgacctgt caacttccgg aaaattcttt aggagacaag aagatggcga aagtagcgtt
     3721 ccttggcctc ggcgtgatgg gctaccccat ggcgggccac ctgctgaaga agggcggcca
     3781 cgaggtcacc gtctacaacc gcagcgcggc caaggcccag cagtgggtca aggagtacgg
     3841 cggcaggagc gcggccaccc cgcgcgaagc cgcgaaggac tgcgacttcg tcatgctgtg
     3901 cgtcggcaac gacgacgacg tgcgctcggt ggtgtacggc gacagcggcg ccctggcggg
     3961 gatgaagaag ggggccatcc tggtggacca cacgacggcc tccgcggttc tggcaagaga
     4021 attatttgaa aaatcaaaat ccgtttccgt gggctttatc gacgcccccg tctcgggcgg
     4081 ccaggcgggt gccgtcaacg gccagctcgg catcatgtgt ggcggcgagg aagcgacgtt
     4141 cgccaaagca aagccggtga tcgacgtcta cgccaagctg gcggcgttga tcggcggccc
     4201 cggcgccggc cagctcacca agatggtcaa ccagatctgc atcgccggcc tggttcaagg
     4261 gctggccgaa ggcttggctt ttgcaaaaag aagcaatctg gatgttgaaa aagtcgtagg
     4321 cgtcatctcc aaaggggcgg cgcagtcctg gcagatggag aaccgctgga agcagatgaa
     4381 cgaggtcaag ttcgacggct tcggcttcgc ggtcgactgg atgcgcaagg acctcggcat
     4441 ctgcatcgag gaatccaaga agaacggcgc ccggctgccg gtcacggcgc tggtcgacca
     4501 gttctacgcc cacatccagg cgcgcggcgg caagcgctgg gacacctcca gcctcatgca
     4561 cctcttgatg aaggactagc gcgccaggct ccggatgtgg aagcagtcgt cttcgcggaa
     4621 caggaaggtc tcgaggaatt tgacgctggc ggcgccgttg acaaggtcgg cgtcgggcgc
     4681 cgagaacggc gccgcgctct gcacggtgtc gagcgcgcgc tggtcgagct cgggatagcc
     4741 gttcgagcgg accaccgcgg cgccgagcag gcggccttcg gcgtcgatgg cgatctcgag
     4801 gatcaccacc gacttcagca tctcctcgcc gggctggcag tacgtgcccg gcgcatgctg
     4861 caggacgtcg agcgcgacga tacgcttata ggcgtcgatg cccgacggcg gcggcgcctc
     4921 gcggcgtggc gcgcacgcgg cgaggacgag cgccaggcc
//
LOCUS       DASYJA010000346         3127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1140391, whole genome shotgun sequence.
ACCESSION   DASYJA010000346 DASYJA010000000
VERSION     DASYJA010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140391"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VGO02_07615"
     CDS             complement(<1..667)
                     /locus_tag="VGO02_07615"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7477588.1"
                     /translation="MCVDAARCLRAYSRAKVVAYYAGYVALEKPEQAVLRTLAECLGS
                     LRMLDIGVGGGRMTEHFAPRVREYKGIDISPAMIDTCRQRFAGRIPPERFAVRDMRDL
                     DGYPAHSFELVLNSYNTMDHLDPEERSRFIGEVRRITAPGGYFCFSTHNIRSLARREP
                     IEWSWRRPHAAVQALLHRARYLKLNRGALEQIPHAEYVIINNGTHDDFELQLYYVQPE
                     AQLA"
     gene            complement(667..1884)
                     /locus_tag="VGO02_07620"
     CDS             complement(667..1884)
                     /locus_tag="VGO02_07620"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEV7477589.1"
                     /translation="MPTEALDLLIVGAGPAGLSAARTAAGCGLRYLVLERGRIAQTIQ
                     DYPLGKPLHSPAQDVEMRWGELYARDARFAARAEVIAHYEMFAAGLHVRTGEAVQSLE
                     RSASGFRVYSDQACYEARRVLVATGGFGVPRRLGVPGEDAARISYWFRDALPYSGKDV
                     LVVGGGNFAAETALWLHEAGARVTLSLRRSSFAPRDGVRDSFTSVKTYNSERLEALAA
                     RGALRILFDSQVIAIGADAATVRTPAATLAVLCCHVFALVGADPDVSLLRGIGAQIAA
                     DGRPVYDPETFETTVPALFVAGHLTRTLHLAPAIALPRRIVRRMAVRQVTARGAGWIA
                     SSLAALVKAARKKSALARAIVRALPPVRRVVQRIDAANVMLDTRPSLVKRLVRRHPGL
                     HRVARRLRALAVS"
     gene            complement(1874..2638)
                     /locus_tag="VGO02_07625"
     CDS             complement(1874..2638)
                     /locus_tag="VGO02_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477590.1"
                     /translation="MSNTCQQFNVLGCHPLQHLAWQGVRRNFGLRQNLRKVFAPLAPM
                     GEPSQYAEGQWVQVLDAEEIKRTLDARSRTRGLLFLAYQWPYCGGVFRVQSVMRRMID
                     DHGFLRPVSRTVLLEGIDCGGVGGTHGCGRRCPIMYRDEWLRPAQPPAPAATLASAVY
                     RRVRPLAEIRATLDWQGKRDGLMFMPEMTRWAGQRFRLARTVQRVFELGRDTSTARTF
                     YILDGLHCSGDVLGGKGPCHRACSILWHGDWLSDAD"
     gene            2801..>3127
                     /locus_tag="VGO02_07630"
     CDS             2801..>3127
                     /locus_tag="VGO02_07630"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEV7477591.1"
                     /translation="MFDLVQKYKRVIQVFLGLIALTFATWGIESYTRFRGATDAVATV
                     NGLDITRREFEDELRRQQDQLRQMFGASFNVEAFDTPEARRVLLDGLVAQRLVSSAAM
                     QSRLTVT"
BASE COUNT          497 a         1066 c         1114 g          450 t
ORIGIN      
        1 cagcgagctg ggcctccggc tgcacatagt agagctgcag ctcgaaatcg tcgtgcgtgc
       61 cgttgttgat gatcacgtac tcggcgtgcg ggatctgttc cagggcgccg cgattcaact
      121 tcagatagcg cgcgcggtga agcaacgcct gcaccgccgc gtgcgggcgg cgccaggacc
      181 actcgatcgg ctcgcgccgc gccagcgagc ggatgttgtg cgtcgagaag cagaaatagc
      241 cgcccggcgc cgtgatgcgc cgcacctcgc caatgaagcg cgagcgctct tccgggtcga
      301 gatggtccat ggtgttgtag ctgttcagga ccagctcgaa gctgtgcgcg ggatagccgt
      361 cgagatcgcg catgtcgcga acggcgaagc gttccggcgg gatcctgccg gcgaagcgct
      421 ggcggcaggt gtcgatcatc gccgggctga tgtcgatgcc cttgtactcc cgcacccggg
      481 gcgcgaagtg ctcggtcatg cgcccgccgc ccacgccgat gtcgagcatg cgcagcgagc
      541 ccaggcactc ggccagggtg cgcagcacgg cctgttcggg cttttccagc gcgacgtagc
      601 cggcgtagta cgccaccacc ttggcgcggg aatatgcgcg caggcagcgc gcggcgtcga
      661 cgcacatcat gaaaccgcga gggctcgcag gcgccgcgcc acgcgatgca gcccggggtg
      721 gcgccgcacc agccgcttca cgagcgaggg ccgtgtgtcg agcatgacgt tggcagcgtc
      781 tatgcgctga accacacggc ggactggcgg cagcgcgcgc acgatggcgc gcgccagcgc
      841 gcttttcttg cgcgcggctt tgacgagtgc ggcgagcgag ctggcgatcc atcctgcgcc
      901 gcgtgcggtg acctgccgga cggccatgcg acgaacgatg cgccggggca gggcaatcgc
      961 gggcgcgagg tgcagcgttc gggtgaggtg tccggccacg aacagcgcgg gaaccgttgt
     1021 ctcgaaagtc tccgggtcgt agaccggccg cccgtcggcg gcgatctgcg cgccgatgcc
     1081 gcgcagcagg ctcacgtcgg ggtcggcgcc gacgagggcg aagacgtggc agcagagcac
     1141 cgccagcgtc gcggcgggcg tgcgcaccgt cgccgcgtcc gcgccgatcg cgatcacctg
     1201 cgaatcgaac aggatgcgca gcgcaccgcg cgcggcgagg gcctcgagcc gctcgctgtt
     1261 gtacgtcttc accgacgtga acgaatcgcg tacgccgtcg cgcggcgcga aggaggagcg
     1321 gcgcagggac agcgtcacgc gcgctccggc ctcgtgcagc cataacgccg tttcggccgc
     1381 gaagttgccg ccgccgacga ccagcacgtc cttgcctgag taggggagcg cgtcgcgaaa
     1441 ccagtaggag atccgcgctg cgtcctcgcc gggcacgccg agccgccgcg gcacgccgaa
     1501 gccgcccgtg gcgacgagca cgcgccgcgc ctcgtagcag gcctggtcgg aatacacgcg
     1561 gaaacccgac gccgagcgct cgagcgactg gacggcctct cctgtgcgga catgcagtcc
     1621 cgccgcgaac atttcgtaat gcgcgataac ctcggcgcgc gcggcaaagc gggcgtcgcg
     1681 cgcgtagagc tcgccccagc gcatctccac gtcctgcgcc ggggaatgga gcggcttgcc
     1741 cagcggatag tcctggatcg tctgggcgat gcgcccgcgc tcgagcacga gatagcgcag
     1801 gccgcagccg gcggcggtgc gggcggcgga cagcccggcg ggaccggcgc cgacgatgag
     1861 cagatccagc gcttcagtcg gcatcgctca gccagtcgcc gtgccagagg atcgagcagg
     1921 cgcggtgaca ggggcccttg ccgccaagga cgtcgcccga gcagtgcagc ccgtcgagga
     1981 tgtagaaggt gcgcgccgtg gacgtgtccc ggccgagctc gaatacgcgc tgcacggtcc
     2041 tcgccaggcg gaagcgctgg ccggcccagc gcgtcatctc gggcatgaac atcagcccgt
     2101 cgcgcttgcc ctgccagtcg agagtcgcgc ggatctccgc cagcggccgc acccggcggt
     2161 agaccgcgct cgccagcgtc gcggccggcg ccgggggctg cgcaggcctg agccattcgt
     2221 cgcgatacat gatcgggcag cggcgtccgc agccgtgggt gccgccgacg ccgccacagt
     2281 cgattccttc gagcaggacg gtgcgcgaga ccggccgcag gaagccgtgg tcgtcgatca
     2341 tccggcgcat cacgctctgc acgcggaaca cgccgccgca atagggccac tggtaggcga
     2401 ggaacagcag gccgcgcgtg cgcgagcgtg cgtccagggt gcgcttgatc tcctcggcgt
     2461 cgagcacctg cacccactgc ccctcggcat attgcgaggg ctcgcccatc ggcgccagcg
     2521 gcgcgaacac ctttcgcagg ttctggcgca ggccgaaatt gcggcgcacg ccctgccacg
     2581 cgagatgctg cagcggatgg cagccgagca cgttgaactg ctgacaggtg ttggacatca
     2641 tgcgagcctc ccggacggac tatcgtgtcc gcgggaatat cttgccgagc gcggaaacag
     2701 gggtcttgca tgcgccggcg agccacgttg cgccgttcgg cctaggacgc gatgctagaa
     2761 ttcgagccgc aagctttcat caacctgtct tccggcccga atgttcgatc tcgtacagaa
     2821 atacaagcgc gtaatccagg tgttcctggg cctgatcgcg ctcaccttcg ccacctgggg
     2881 aatcgagtcc tacacccgct tccgcggcgc caccgacgcg gtggcgacgg tgaacgggct
     2941 cgacatcacg cggcgcgagt tcgaggacga gctgcgccgg cagcaggacc agctgcgcca
     3001 gatgttcggt gcctcgttca acgtcgaggc gttcgacacg ccggaggcgc ggcgcgtgct
     3061 gctcgatggc ctggtcgcgc agcgcctggt gtcctcggcg gcgatgcaat cccgcctcac
     3121 cgtgacc
//
LOCUS       DASYJA010000347         4240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_50961, whole genome shotgun sequence.
ACCESSION   DASYJA010000347 DASYJA010000000
VERSION     DASYJA010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50961"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VGO02_07635"
     CDS             complement(<1..228)
                     /locus_tag="VGO02_07635"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEV7477592.1"
                     /translation="MNPFQISRVLDAPRDRVWKSWTEAERLKHWWGPAGFKVHTCKVD
                     LRPGGSFLYGMTAPDGIDVWGRFVYREIKAPD"
     gene            complement(225..581)
                     /locus_tag="VGO02_07640"
     CDS             complement(225..581)
                     /locus_tag="VGO02_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HEV7477593.1"
                     /translation="MYVDGFVLPVPKRKRSAYLKMARKAGKVWREHGALDYKECVADD
                     VKPGKWTSFPQSVKLKKDEVVWFSFIVYKNRKQRDRVMAKVMKDKRLASIMEPKSMPF
                     DGKRMIFGGFKVAVDL"
     gene            complement(598..1038)
                     /locus_tag="VGO02_07645"
     CDS             complement(598..1038)
                     /locus_tag="VGO02_07645"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEV7477594.1"
                     /translation="MEKILMRESLVLVRTFTVVPKKVWRAWIRPDALRVWFGQSGAAG
                     WKAELDVRVGGRYHLVMQHPRGSYYDVRGEYREVVPDRRLVFTWEQRGSDLAEGQALV
                     SVELRPAAGGTELRFVLEPLFDPTARAAWEGDFRRLARMLQPKD"
     gene            complement(1041..1496)
                     /locus_tag="VGO02_07650"
     CDS             complement(1041..1496)
                     /locus_tag="VGO02_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEV7477595.1"
                     /translation="MEQAKLAEKLALSLQRRYPVAPEKVWRAWTDPQALKQWWGPGGP
                     EAVSLVQLDVRVGGRFRIVFGGPQGDEHEVQGTYKEVVANRRLAFTWTWPRTTPERES
                     LVTITFKPAAGGTELEFVHAQHFDEKVRDGHLRGWTEAFVKLERYLQSV"
     gene            complement(1391..1834)
                     /locus_tag="VGO02_07655"
     CDS             complement(1391..1834)
                     /locus_tag="VGO02_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV7477596.1"
                     /translation="MVKYSDDDLDTVFAALSDRTRRHVLQSLAAGDRQVTELANEHEM
                     SLPGFMKHLRVLADAGLIARSKEGRVVSCELSAQPMQAASTWMSRYEKFWTEKLDSLA
                     RYLYQQEELHTWNKPSSPKSSRSASSGATPLPRKRSGGRGPTRKR"
     gene            complement(1889..3421)
                     /locus_tag="VGO02_07660"
     CDS             complement(1889..3421)
                     /locus_tag="VGO02_07660"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF002868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEV7477597.1"
                     /translation="MSLLADVVRTSTEVASTSSRLAKVRSLADCVKRLDAEEVEVAIP
                     YLSGDIRQGKLAVGFQMLRAARLPANAPSLTLIEVDSAFANLKAVKGKGAAQRREASL
                     KSLFERATAEEQDFLTRLIIGELRQGALEGVMLDAVASAAGLPAAEVRRAATFAGGVA
                     QVARAALAGGAGALGQFSIRLMQPVMPMLAQPAEDLAAALAALGSALLEWKLDGARVQ
                     VHKAGDEVKVYTRSLNEVTAAVPEVVEAVRRAKAQSLILDGEVIALRPDGRPLPFQTT
                     MRRFSRKLDIEALRHELPLSVFFFDCLFKDGESLVDRGTADRRRLLEGVFAGDNVVPG
                     LATADLAKAQAFYEDVLARGHEGVMAKAPDAPYEAGRRGASWFKVKRAKTLDLVVLAA
                     EWGSGRRRGFLSNLHLGARDPAGGFVMLGKTFKGLTDETLAWQTRELQAREVQRDEWT
                     VYVRPELVVEIAFNDLQESPHYPGGLALRFARVKGYRPDKSAAEADTIDAVRQIYQNS
                     LK"
     gene            complement(3421..4240)
                     /gene="accD"
                     /locus_tag="VGO02_07665"
                     /pseudo
     CDS             complement(3421..4240)
                     /gene="accD"
                     /locus_tag="VGO02_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
BASE COUNT          669 a         1493 c         1349 g          729 t
ORIGIN      
        1 gtccggcgcc ttgatctcgc ggtacacgaa gcggccccac acgtcgatgc cgtcgggcgc
       61 ggtcatgccg tagagaaagc tgccgccggg gcgcaggtcg accttgcagg tatgcacctt
      121 gaagccagcc ggtccccacc agtgcttgag ccgttcggcc tcggtccagg acttccagac
      181 ccggtcgcgc ggcgcgtcga ggacgcggct gatctggaaa gggttcacag gtccaccgca
      241 accttgaagc cgccgaagat catgcgcttg ccgtcgaacg gcatgctctt cggctccatg
      301 atgctggcga ggcgcttgtc cttcatcacc ttggccatca cgcggtcgcg ctgcttgcgg
      361 ttcttgtaca cgatgaacga gaaccagacc acctcgtcct tcttcagctt cacgctttgc
      421 gggaacgagg tccattttcc aggcttgacg tcgtccgcca cgcactcctt gtagtcgagc
      481 gcgccgtgct cgcgccacac tttcccggcc ttgcgcgcca tcttcagata ggccgagcgt
      541 tttctcttcg gcaccggcaa tacgaatccg tcaacgtaca tgttgcctcc ttggcagtta
      601 gtctttcggt tgaagcatcc gggcgagccg ccggaagtcg ccttcccagg cagcgcgcgc
      661 cgtcggatcg aacagcggct ccagcacgaa gcgcagctcg gtgccgcccg cggccgggcg
      721 cagctcgacg ctgaccagcg cctggccctc ggcgagatcg ctgccgcgct gctcccaggt
      781 gaacaccagc cgccggtccg gcaccacctc gcgatattcg ccgcgcacgt cgtagtagga
      841 gccgcgcgga tgctgcatca cgaggtggta gcggccgcct acgcgtacgt ccagctcggc
      901 cttccagccc gctgcgccgg actggccgaa ccagacgcgc agcgcgtcgg gcctgatcca
      961 ggcgcgccac accttcttcg gcacgaccgt gaaagtgcgg accaggacga gcgactcgcg
     1021 catcagtatc ttttccattg ctatacgctc tgcaagtagc gctccaactt gacgaacgct
     1081 tcggtccagc cgcgcaggtg accatcgcgc accttctcgt cgaagtgctg tgcatgcacg
     1141 aattcgagct cggtgccgcc cgccgccggc ttgaacgtta tcgtgaccag cgactcgcgc
     1201 tccggtgtgg tgcgcggcca ggtccaggtg aaggcgagcc tgcggttcgc caccacttcc
     1261 ttgtaggtgc cctgcacctc gtgctcgtcg ccctgcggcc cgccgaatac gatacggaaa
     1321 cggccgccta cgcgcacgtc gagctgcacc agcgagaccg cctcggggcc gccgggcccc
     1381 caccactgct tcagcgcttg cgggtcggtc cacgcccgcc agaccttttc cggggcaacg
     1441 gggtagcgcc gctggaggct gagcgcgagc ttttcggcga gcttggcttg ttccatgtgt
     1501 gcagttcctc ttgctggtac aggtagcggg ctagcgaatc gagtttctcg gtccagaact
     1561 tctcgtagcg cgacatccag gtggaggcgg cctgcatggg ctgcgcggac agctcgcagc
     1621 tcaccaccct gccctccttg gagcgcgcga tcagcccggc gtccgcgagc acccgcaggt
     1681 gcttcatgaa gccgggcagc gacatctcgt gttcgttcgc cagctcggtg acctgccggt
     1741 cgcccgcggc gagcgactgc agcacgtggc gccgggtacg gtcggacagc gcggcaaaca
     1801 ccgtgtcgag atcgtcgtct gaatacttaa ccattaggtt tagtatcagg cagcgccggg
     1861 gcggctgtca actacttttt ggccggggct atttcaggct gttctgataa atctgccgga
     1921 cggcgtcgat ggtgtccgcc tctgcggcgg acttgtccgg gcgatagccc ttgacccgcg
     1981 cgaagcgcaa cgcgagcccc ccagggtaat ggggactctc ctgcaggtcg ttgaacgcga
     2041 tctccacaac aagctcaggc cgcacgtata cggtccactc gtcgcgctgc acttcgcgtg
     2101 cctgcagctc gcgcgtctgc cacgccaggg tctcatcggt cagtcccttg aacgtcttgc
     2161 ccagcatgac gaagccgccc gccgggtcgc gcgcaccgag gtgcaggttg gagaggaagc
     2221 cgcgccggcg accgctaccc cactccgccg ccaggaccac gaggtcgagc gtcttcgcgc
     2281 gcttcacctt gaaccagctg gcgccccgcc ttccggcttc gtacggcgca tccggcgctt
     2341 ttgccatgac gccctcatgg cccctggcga gcacgtcttc gtagaacgcc tgtgccttgg
     2401 caaggtccgc ggtcgccagg cccgggacga cgttatcccc tgcaaatact ccttcaagga
     2461 gccgcctgcg atcggcggtt ccacggtcca ccagcgactc tccgtccttg aagagacagt
     2521 cgaaaaagaa caccgacaga ggcagctcgt ggcgcagcgc ctcgatatcg agcttgcggc
     2581 tgaagcggcg catcgtcgtc tggaacggca gcgggcgccc gtcgggccgc agcgcgatca
     2641 cctcgccgtc gaggatcagg gactgcgctt tggcgcgcct cacggcttcc actacctcgg
     2701 gaaccgccgc cgtgacttcg ttgaggctgc gcgtgtagac cttcacctcg tcgcccgcct
     2761 tgtgcacctg gacgcgtgcg ccgtcgagct tccattcgag tagcgcgctg ccaagggcag
     2821 cgagcgcggc ggcgaggtcc tcggccggct gcgccagcat gggcatcact ggttgcatca
     2881 accggatcga gaactgcccg agcgcgccgg cgccgcccgc gagcgccgcc cgcgccacct
     2941 gcgcgacgcc gccggcgaag gtggcggcgc ggcgcacctc ggcagcaggc aggccggccg
     3001 ccgatgcgac cgcatcgagc atcacgcctt cgagcgctcc ttgccgcagc tcgccaatga
     3061 tcaggcgggt caggaaatcc tgctcttcgg cggtcgccct ttcaaacagg ctcttcagcg
     3121 acgcttcgcg ccgctgcgca gcgcccttgc ctttgacggc cttcagattc gcgaatgcgg
     3181 aatcaacttc gatcagcgta agtgaaggag cgttagcggg taagcgtgcg gcgcgcagca
     3241 tctgaaagcc gacggccagc ttgccctggc gaatgtcgcc gctcaggtag ggaatcgcga
     3301 cctcgacctc ttcggcgtcg aggcgtttca cgcagtcggc caggctgcgc accttcgcca
     3361 ggcggctcga cgtgctcgcc acctcggtgg aggtgcgcac cacgtcggcc agcagggaca
     3421 cgccgcagcg tcggagggca ggcggagcag cagggaaagg acgctgtgca gcttgtcgcg
     3481 caactggcgg cggtcgacga tcatgtcgat cgcgcccttc tccagcagga actcggcgcg
     3541 ctggaagccc tcgggcagct tctgccgcac cgtctgctcg atgacgcgcg gcccggcaaa
     3601 gccgatcagc gccttcggct cggcgatcac cacgtcgccg atcatggcga agctcgccga
     3661 aacgccgccc atggtcggat cggacagcac cgagacgaac ggcagcttgc gctcggcgag
     3721 gtcggtgagc gacgcagtgg tcttcgccat ctgcgcgagc gacagcaggc cttcctgcat
     3781 gcgcgcgccg cccgaggcgg tgacgcagac gaacggcagc ttctgctcga tcgccacgcg
     3841 caccccgcgc acgaagcgct cgcccaccac cgagcccatc gagccgccca tgaagtcgaa
     3901 ctcgaacgcg gcgcacacca cgccgagcgc cttgatcgag ccctgcatca ccaccagcgc
     3961 gtcgccctcg gcggtctgct cctgcgcttc cgccaggcgc tcgaagtact tgcgcgagtc
     4021 cttgaacttg agcgtgtcga tcggcagcac ctcggcgccg atctcgaagc ggccatccgg
     4081 gtcgagcagc gcatcgaggc gcggccgcgc cgccaggcgc tcgtggtggc cgcacttcgg
     4141 gcacacgttc tggttcttct cgaggtcggt ggcgtagagc acggcgtcgc aggccgggca
     4201 cttcgtccac aggccttccg gcacggtctt gcgcgtagtg
//
LOCUS       DASYJA010000348         2271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2320427, whole genome shotgun sequence.
ACCESSION   DASYJA010000348 DASYJA010000000
VERSION     DASYJA010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2320427"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..587)
                     /locus_tag="VGO02_07670"
     CDS             complement(90..587)
                     /locus_tag="VGO02_07670"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEV7477598.1"
                     /translation="MSQPPRPRIYLVAAVASNGIIGANGRLPWHFPEELKHFKEVTLG
                     HPVIMGRRTWESLKGALPGRKNIVVTRTPGYRAPGASVAVSLQAALALCADAPKAMVI
                     GGSQLFAESLPIAAGLELTEIRRDYPGDTWFPEYDRAPWKEIRREPHAAPDGTRFDYV
                     LYEKA"
     gene            629..2092
                     /locus_tag="VGO02_07675"
     CDS             629..2092
                     /locus_tag="VGO02_07675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV7477599.1"
                     /translation="MAAHWVPYPRSFLGLLLTGFTLVAVPLVSALAYSAWNTERLAEQ
                     SRSAVFSASQAARASRSLVNRIGSIERLAPQVAMLDDPELVVDYRRVHGSFRQVAAEL
                     MQLPLDDAQRAALLKTVAQEQDLYDRLTARPRVRLDVRQIGRLTDDLAENAYEVLAIS
                     YLVADREVVRLRASAEIVQRQLILLVLFGTAVALATALVLSRYIARPIAELDGSIRQL
                     GDADFSRPIKVRGPEDLQTLGERLDWLRRRLGELEAEKNRFLRHLSHELKTPLTALRE
                     GAELLNDQVGGPLAPPQRQVVTIMRDNSVKLQRLIEDLLDYQRALHAAASLEPRPVAL
                     DALAREVIRSHELAMRAKAQALALDLDKVTVEADPEKLRSILDNLLGNAVKFTPGGGT
                     ITVAVHLGSLGAEAVIDVVDSGPGVPMEERDAIFNSFFRGRAQASGRIEGTGLGLAIA
                     REFVEAHGGRISVVPRKDGGHFRVTLPRKAVRVLAAA"
     gene            2089..>2271
                     /locus_tag="VGO02_07680"
     CDS             2089..>2271
                     /locus_tag="VGO02_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477600.1"
                     /translation="MKTPAALVCLVLACLLPVGCSVLQASAADTVVGEALVAARAPAA
                     EQKAALARAQAAFTSDM"
BASE COUNT          326 a          792 c          791 g          362 t
ORIGIN      
        1 atcgccaacg ccggcgccta taccacggcc tacgcgagcc agttcaacgg cttcccgtta
       61 cccgaggtgc gggtgttcga gtcgaaaaac taggcttttt cgtagaggac gtagtcgaac
      121 cgcgtgccgt ccggggcggc atgcggttct cgtcttatct ccttccacgg cgcccggtcg
      181 tactccggga accaggtatc gccggggtaa tccctgcgga tctcggtcag ctccaggccg
      241 gcggcgatcg gcagcgattc cgcgaacagc tggctgccgc cgatgaccat cgccttcggt
      301 gcgtccgcgc atagcgccag cgccgcttgc agcgagacgg ccaccgaggc gccgggcgcc
      361 cggtagcccg gcgtgcgcgt caccacgatg ttcttgcggc ccggcagggc gcctttgagc
      421 gattcccagg tccgccggcc catgatgacc gggtggccga gcgtgacttc cttgaagtgc
      481 ttcaattcct cgggaaagtg ccacggcaga cggccgttgg cgcctatgat cccattcgaa
      541 gcgacggcgg cgaccaggta gatccgcggc cgggggggct gtgacattgg tcctcgcagg
      601 tagcgatttt tgttgaaatt ataaggacat ggctgcgcac tgggtccctt atccgcgctc
      661 ttttctcggg ctgctgctga ccggcttcac gctggtcgcg gtgccgctgg tcagcgcgct
      721 cgcctactcg gcgtggaata ccgagcgcct ggccgagcaa agccgcagcg cggtgttcag
      781 cgcctcgcag gccgcgcgcg ccagccgctc gctggtgaac cgcatcggct cgatcgagcg
      841 gctggcgccg caggtggcga tgctcgacga cccggagctg gtcgtcgact accggcgcgt
      901 gcacggcagc ttcaggcagg tagccgcgga gctcatgcag ctgcccctcg acgacgcgca
      961 gcgcgccgcg ctcctgaaga ccgtcgcgca ggagcaggac ctgtacgacc gcctcacggc
     1021 gcggccccgc gtgcgcctcg atgtgcgcca gatcggccgg ctgaccgacg acctggccga
     1081 aaacgcctac gaagtgctcg ccatcagcta tctggtggcc gaccgggaag tggtgcgcct
     1141 gcgcgccagc gccgagatcg tgcagcgcca gctgatcctg ctggtgctgt tcggcacggc
     1201 ggtggcgctc gccaccgcgc tcgtcctttc gcgctacatc gcgcgcccga tcgccgagct
     1261 cgacggctcc atccgccagc tcggcgacgc cgatttctcg cggccgatca aggtgcgcgg
     1321 ccccgaggac ctgcagacgc tgggcgagcg cctggactgg ctgcggcgca ggctgggcga
     1381 gctcgaggca gagaagaacc gcttcctgcg ccacctgtct catgagctta agacgccgct
     1441 cacggcgttg cgggaggggg cggagctgct gaacgaccag gtcggcgggc cgctcgcgcc
     1501 gccccagcgt caggtggtga cgatcatgcg agacaacagc gtcaagctgc aacggctcat
     1561 cgaggacctg ctcgactacc agcgcgcgct gcatgcggcg gcgtccctcg agccgcgtcc
     1621 ggtcgcgctc gacgccctgg cgcgcgaggt tatccgctcg cacgagctcg cgatgcgcgc
     1681 caaggcgcag gcgctcgccc tggacctcga caaggtgacg gtagaggccg acccggagaa
     1741 gctgcgctcg atcctggaca acctgctcgg caacgccgtc aagttcaccc cgggcggcgg
     1801 cacgatcacc gtcgcagtcc atctgggctc cctcggcgcc gaggcggtga tcgacgtggt
     1861 cgattccgga cccggcgtgc cgatggaaga gcgcgacgcg atcttcaatt cgttcttccg
     1921 cggccgggcg caggcgagcg ggcgcatcga gggcaccggc ctcggcctgg ccatcgcgcg
     1981 cgagttcgtc gaggcgcacg gcgggcggat cagcgtggtg ccgcgcaagg acggcggcca
     2041 tttccgcgtc accctgccgc gcaaggcggt gcgcgtcctg gcggcggcat gaagactccg
     2101 gccgccctcg tctgcctggt gctcgcctgc ctcttgcccg tcggttgttc ggtgctgcag
     2161 gcgagcgccg cggatacagt ggtgggcgaa gccttggtcg ccgcgcgcgc ccccgccgcg
     2221 gagcagaagg cggcgctcgc ccgggcgcag gcggccttca cctccgacat g
//
LOCUS       DASYJA010000349         1644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_141328, whole genome shotgun sequence.
ACCESSION   DASYJA010000349 DASYJA010000000
VERSION     DASYJA010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141328"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VGO02_07685"
     CDS             <1..537
                     /locus_tag="VGO02_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEV7477601.1"
                     /translation="AMVFALARRTLSWRALYAALVESARTTSMLFMILIGALMFAEFV
                     NITSMPTDLKNFVSRFQVSPVAVVAVIMIIYVLLGTAMEELSMILLTVPVFFPLVVSL
                     GVDPIWFGILIVVVVEIGLISPPVGMNLFVLNTLLPQVPTRVIFRGVLPFMAIDCVRL
                     AILIAFPILSLWLPGFMR"
     gene            534..1280
                     /locus_tag="VGO02_07690"
     CDS             534..1280
                     /locus_tag="VGO02_07690"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEV7477602.1"
                     /translation="MNQRDKDYFKERAAREMEEDLPVVEGSTAELLAHTCRILAAEGH
                     ESGLAGQITARGEKPGTFWTLQLGYGFDEAGAERMVLVDGDLNPLTGGRVNAGVRFHV
                     WIYNHRPEVNAIVHTHPPHAAALSLTGKPLKAAHMDSSMLYGNCAYLPDWPGLPVADD
                     EGRIISQALGGNKAILLANHGLLTTGGYVEEAAYLAVFFERAARMELRALAAGGIREP
                     RPELLREAGEFLLKPQIVKGTFAYWARRCG"
     gene            1277..>1644
                     /locus_tag="VGO02_07695"
     CDS             1277..>1644
                     /locus_tag="VGO02_07695"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV7477603.1"
                     /translation="MSERALAFTFLALANLFWAGNWVLGRALRDAVDPISLNFYRWVI
                     AVVVLAPFALPGLAAQRAVIRRHAGRLALLALVGVSVFQSLIYLGLQSTTAVNAVLIN
                     CAGPLFILLCAWALDGERATL"
BASE COUNT          226 a          596 c          522 g          300 t
ORIGIN      
        1 gccatggtgt tcgcgctggc gcgcaggacg ctcagctggc gcgcgctcta tgccgcgctc
       61 gtcgagagcg cgcgtaccac ctcgatgctg ttcatgatcc tcatcggcgc gctgatgttc
      121 gccgagttcg tcaacatcac ctcgatgccc acggacctga agaatttcgt gtctcgcttc
      181 caggtgagcc cggtggcggt ggtggcggta atcatgatca tctatgtcct gctcggcacg
      241 gcgatggagg agctttccat gattctcctt acggtgccgg tgttcttccc gctggtcgtg
      301 agcctcggcg tcgacccgat ctggttcggc atcctcatcg tcgtcgtcgt ggagatcggc
      361 ctcatcagcc cgccggtggg catgaacctg ttcgtcctca acaccctgct gccgcaggtg
      421 cccacgcgcg tcatcttccg cggcgtgctg ccgttcatgg ccatcgactg cgtgcgcctc
      481 gcgatcctga tagccttccc gatcctctcg ctctggctgc cgggcttcat gcgatgaatc
      541 aacgcgacaa agattacttc aaggaacgcg ccgcgcgcga aatggaggaa gaccttccgg
      601 tggtggaagg ctccaccgcg gagctgctcg cgcacacctg ccgcatcctc gccgccgaag
      661 ggcacgagtc gggcctcgcc ggccagatca ccgcgcgcgg cgagaagccg gggaccttct
      721 ggacgctgca gctcggctac ggcttcgacg aagcgggcgc cgagcgcatg gtgctggtcg
      781 atggcgacct caatccgctg accggcgggc gcgtgaatgc cggcgtgcgc ttccacgtct
      841 ggatctacaa ccaccggccc gaagtgaacg ccatcgtgca cacccacccg ccgcacgccg
      901 cggcgctctc cctcaccggc aagccgctca aggccgcgca catggattcg tcgatgctct
      961 acggcaactg cgcctacctg cccgactggc ccggcctgcc ggtcgccgac gacgagggcc
     1021 gcatcatctc gcaagcgctc ggcggcaaca aagccatcct cctcgccaac cacggcctgc
     1081 tcaccaccgg cggctacgtc gaggaagcgg cctacctcgc ggtgttcttc gagcgcgccg
     1141 cgcgcatgga gctgcgcgcc cttgccgccg ggggcatccg cgagccccgg cccgagctgc
     1201 tgcgcgaggc cggcgaattc ctcctcaagc cgcagatcgt aaagggaacc ttcgcctact
     1261 gggcgcgccg ctgcgggtga gcgagcgcgc gctggctttc accttcctcg cgctggccaa
     1321 cctgttctgg gccggcaact gggtcctcgg ccgggcgctg cgcgacgcgg tcgacccgat
     1381 ctcgctgaac ttctaccgct gggtcatcgc cgtggtggtg ctcgcgccgt tcgcgctgcc
     1441 cggtctcgcc gcgcagcgcg cggtcatccg ccggcacgcc gggcgtctcg ccctgctcgc
     1501 gctggtcggg gtgtcggtgt tccagagcct gatctatctc ggcctgcagt cgacgaccgc
     1561 ggtgaacgcc gtcctcatca actgcgcggg gccgctgttc atcctgctct gcgcctgggc
     1621 gctcgacggc gagcgtgcca cgct
//
LOCUS       DASYJA010000350         2593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2320557, whole genome shotgun sequence.
ACCESSION   DASYJA010000350 DASYJA010000000
VERSION     DASYJA010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2320557"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..1130
                     /locus_tag="VGO02_07700"
     CDS             207..1130
                     /locus_tag="VGO02_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV7477604.1"
                     /translation="MTFSLVFEQLLNGLQYGVMLFLMAAGLTLVLGIMNMVNLAHGSL
                     YMVGAYLAVAATQWTGSYVAGLLLGLAGTLLVGMLVEVIALRALYARDHLDQVLATFG
                     LILFFNELVAILWGRAALFASPPDWLKGHVELFTGSRYPLYRLVIIGVGLLVGILLWY
                     VVTRTRLGMLIRAGASNRAMVGALGVNIRLLYTVVFGFGAALAGLAGIMAGPIYNVQP
                     GMGELILIQVFVVIVIGGIGSIRGALLGALIVGIVDTLGRAFLKPLLGHLISPPAAES
                     AGPALASMLIYLIMAVVLAIRPAGLFPSRGG"
     gene            1130..2056
                     /locus_tag="VGO02_07705"
     CDS             1130..2056
                     /locus_tag="VGO02_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV7477605.1"
                     /translation="MKRTLIFWGVLALVLIALPPVMLALKQPFYLDLVRRIMILAIAA
                     VSLNLILGYGGMVSFGHAAYIGIGAYAVGILGFYGISNGWAHMAVAIGASALVALAIG
                     AVSIRTSGIYFIMITLAFTQMLYYLGISIAEFGGDDGMRLKSKSQFGLVDLRDPVVFY
                     YVALALMLLSVYLVFRIVNSRFGMVLRATRSNDARSRAIGFSPYPYRLAAFVISGALC
                     GLAGALYANHTNYITPGLMSWLQSGDLMFMVILGGIGSTAGPVLGSFALVLVEDLLKG
                     FTQYYYVILGPLLVLSVIFFKRGLAGIFFRNE"
     gene            2049..>2593
                     /locus_tag="VGO02_07710"
     CDS             2049..>2593
                     /locus_tag="VGO02_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7477606.1"
                     /translation="MNSLLTVEKLSKSFGALRASDAIDFDVAEGETHAVIGPNGAGKT
                     TFISQLAGNLRPDAGRIAFAGEDITRLPAARRARRGLARSFQITSVYLEFSALQNVAL
                     AVQARSGHSFSFWRDARNDAALLEPARLVLRDVGIDEAKFNMLAANLAHGEQRQLEVA
                     MALATRPRLLLLDEPMAGMGT"
BASE COUNT          378 a          872 c          825 g          518 t
ORIGIN      
        1 agttcgagta caacgtcaac caccacccga tccagaactt ctacctgctg aagacggtga
       61 agaccggcgg cggcgacatc gagatgcaca tcgagaagac catcttcgag aagcacaagg
      121 actcgtacta ccaggattgc aagatgaaat ggtagttttt aaaaaatagc ttttccagga
      181 agggcgcgca ggcgcccttt tttttgatga ccttttcgct cgtattcgag cagttgctga
      241 acggcctgca gtacggcgtc atgctgttcc tcatggcggc cgggctgacg cttgtgctcg
      301 gcatcatgaa catggtcaac ctggcgcacg gctcgctcta catggtcggc gcctacctcg
      361 ccgtagcggc gacgcagtgg accggctcct acgtcgccgg cctgctgctc ggcctcgccg
      421 gcacgctgct ggtcggcatg ctggtggagg tgatcgcgct gcgcgcgctg tacgcgcgcg
      481 accacctcga ccaggtgctg gcgaccttcg gcctgatcct gttcttcaac gagctggtcg
      541 ccattctgtg gggacgcgcg gcgctcttcg cctcgccgcc ggactggctg aaggggcacg
      601 tcgagctgtt taccggctcg cgttatccgc tgtatcggtt ggtgatcatc ggggttggtc
      661 tgctggtagg aattcttttg tggtacgtgg tgacgcgcac gcgcctcggc atgctgatcc
      721 gcgccggcgc ctccaaccgc gcgatggtcg gcgcgctcgg cgtgaacatc cgcctcctct
      781 acaccgtcgt cttcggcttc ggcgcggcgc tggccggcct cgccgggatc atggcggggc
      841 cgatctacaa cgtgcagccg gggatgggcg agctgatcct gatccaggtg ttcgtggtga
      901 tcgtgatcgg cggcatcggc tcgatccgcg gcgcgctgct cggcgccctg atcgtcggca
      961 tcgtcgacac gctgggccgc gcctttctca agccgctgct cgggcacctg atctcgccgc
     1021 cggcggccga gtcggccggg ccggcgctcg cctccatgct gatctacctg atcatggcgg
     1081 tggtgctggc gatccgccct gcggggctgt tcccctcccg gggcggatag tgaagaggac
     1141 gctcattttc tggggcgtgc tggcgctggt gctgatcgcg ctgccgccgg tcatgctcgc
     1201 gctcaagcag ccgttctacc tcgacctcgt gcgccgcatc atgatcctcg ccatcgccgc
     1261 ggtcagcctc aacctgatcc tcggctacgg cggcatggtg tccttcggcc acgccgccta
     1321 catcggcatc ggcgcctatg cggtcggcat cctcggcttc tacggcatca gcaacggctg
     1381 ggcgcacatg gcggtcgcca tcggcgcctc ggcgctggtc gcgctcgcca tcggcgcggt
     1441 gtcgatccgc accagcggca tctacttcat catgattacc ctcgcgttca cgcagatgct
     1501 ctactacctc ggcatctcga tcgccgagtt cggcggcgac gacggcatgc gcctgaagag
     1561 caagagccag ttcggcctgg tcgacctgcg tgacccggtg gttttctact atgtggcgct
     1621 cgcgctgatg ctgctgtcgg tctacctggt tttcaggatc gtgaattcca gattcggcat
     1681 ggtgcttcgc gccaccaggt cgaacgacgc ccgctcgcgc gccatcggct tctcgcccta
     1741 cccgtaccgc ctcgcggcct tcgtcatctc cggggcgctg tgcggcctcg ccggggcgct
     1801 gtacgccaac cacaccaact acatcactcc aggcctgatg agctggctgc agtccgggga
     1861 cctcatgttc atggtgatcc tgggcggcat cggcagcacc gccgggccgg tgctcggctc
     1921 gttcgccttg gtgctggtcg aggacctgtt gaagggcttc acccagtact actacgtgat
     1981 cctcggcccg ttgctggtgc tgtcggtgat cttcttcaag cgcggcctgg cgggcatctt
     2041 tttccgcaat gaatagtctt ctcacggtag aaaaactatc caagagcttc ggcgccttgc
     2101 gcgcgagcga cgctatcgac ttcgacgtag ccgagggcga gacgcacgcg gtcatcgggc
     2161 cgaacggcgc cggcaagacg accttcatca gccagctcgc cggcaacctg cggcccgacg
     2221 ccgggcgcat cgccttcgcc ggcgaggaca tcacgcgcct gccggcggcg cgccgcgcgc
     2281 gcaggggcct ggcgcgctcc ttccagatca ccagcgtgta cctcgagttc agcgccctgc
     2341 agaacgtcgc gctcgcggtg caggcgcgct cgggccacag cttcagtttc tggcgcgatg
     2401 cgcgcaacga tgccgcgctt ctcgagcccg cgcgccttgt actccgagat gtaggaattg
     2461 acgaagcgaa attcaacatg ctggcggcca acctggcgca tggcgagcag cgccagctcg
     2521 aggtcgcgat ggccctcgcc acgcgcccgc gcctgctgct gctcgacgag ccgatggccg
     2581 gcatgggcac cga
//
LOCUS       DASYJA010000351         3305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1412594, whole genome shotgun sequence.
ACCESSION   DASYJA010000351 DASYJA010000000
VERSION     DASYJA010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412594"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="VGO02_07715"
     CDS             complement(<1..158)
                     /locus_tag="VGO02_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEV7477607.1"
                     /translation="MKPSLQFRLSQHLTLTPQLQQSIRLLQLSTLELNQEIERLLQEN
                     PLLEKTED"
     gene            complement(189..911)
                     /gene="lptB"
                     /locus_tag="VGO02_07720"
     CDS             complement(189..911)
                     /gene="lptB"
                     /locus_tag="VGO02_07720"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEV7477608.1"
                     /translation="MSQLNAQSLRKRYKSRTVVADVSLEVSSGEVVGLLGPNGAGKTT
                     CFYMIVGLVPADAGSIHLDTQDLTHLPIHRRARMGISYLPQENSVFRRLTVEENVQAI
                     LELQGLQSGEIEERLAQLLKDLNIAHLRKHSAISLSGGERRRLEIARALGTKPAFILL
                     DEPFAGVDPIAVLDIQRIIRFLKERGIGVLITDHNVRETLGICDRAYIINEGAVLASG
                     HPEEIVYNDSVRRVYLGENFRM"
     gene            complement(908..1426)
                     /gene="lptA"
                     /locus_tag="VGO02_07725"
     CDS             complement(908..1426)
                     /gene="lptA"
                     /locus_tag="VGO02_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEV7477609.1"
                     /translation="MKFFWVFLLVFSLSAQAEKADKEKPTELEANRMSADEVRRLNIF
                     DGNVVLVKGTMRLLAERIVVRQDAEGYQYATATGKPVRFRQRQDPRPPEKEGVWLDGE
                     ALRIEIDERASTVKLFENARVNRGGDQVAGNYILVDQTSEFFSVSAGKDGDQRVKMII
                     QPKPAATPAPKK"
     gene            complement(1423..2001)
                     /gene="lptC"
                     /locus_tag="VGO02_07730"
     CDS             complement(1423..2001)
                     /gene="lptC"
                     /locus_tag="VGO02_07730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEV7477610.1"
                     /translation="MRVPTTRLFPLGLMLALAALTFYLDRAVNDEDTHPAMRRHDPDY
                     IVVNFTTTTYNRDGAAESTLSASRMEHFPDDDTTELTAPRVVQTKPDQPRMTLRADRG
                     KLSRDGEDVYLYDNVVLEREAGAGHTAARLTTDYLQVVRDRSLVRTDRPVNIVEETRA
                     LSGKGMEYSNETQHFTLNADVRGRFEAKSAPQ"
     gene            complement(1998..2447)
                     /locus_tag="VGO02_07735"
     CDS             complement(1998..2447)
                     /locus_tag="VGO02_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEV7477611.1"
                     /translation="MIFDVDGVLTDGTLWYGPAGEALKAFSAHDGHGIKLLAAAGVAV
                     ALLSGRRSKAVAVRARELGIKRVFQGVDDKLSVFEKLLKKERLKPESTGFMGDELVDL
                     PVLERCGFACAPREAPEAVRSRVHYVTEAPAGRGAVRELCDLMVRTA"
     gene            complement(2495..>3305)
                     /locus_tag="VGO02_07740"
     CDS             complement(2495..>3305)
                     /locus_tag="VGO02_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEV7477612.1"
                     /translation="GHIARKIASTLASTGTPAYFVHPAEASHGDLGMVDSGDVFIAIS
                     YSGGSEELLDIVPLVKRRGAKLIAITGNENSALARDADVHLDAAVAQEACPLNLAPTA
                     STTAALALGDALAVALLDARGFTKDDFARSHPRGRLPRQALLHVSDVMRKGERLPVVP
                     ASAPLKEALREMTRGGIGMTAVRDAAGRVGGIFTDGDLRRALEKGADFAAARVADVMT
                     TGPRVIRADALAVEAVQLMEAHKVNQLLVVDAGGELVGALNMHDLFRAKVI"
BASE COUNT          490 a         1112 c         1148 g          555 t
ORIGIN      
        1 tcgtcctcgg tcttctcgag cagcgggttt tcctggagca ggcgctcgat ttcctggttg
       61 agctcgagcg tcgaaagttg caacagccgg atggactgct gcagctgggg agtcagcgtg
      121 aggtgctggg agagccggaa ctgaagcgag ggcttcatca gaaaaccagg gggacgacct
      181 cgggcaacct acattcggaa attctcgccc agatatactc ggcgaacgct gtcattatag
      241 acaatctcct cgggatggcc cgaagcgagc acggccccct cgttgataat gtacgcgcga
      301 tcgcagatgc cgagcgtttc gcgaacgtta tggtctgtta tgagcacccc gatgccgcgc
      361 tccttgagga acctaatgat cctctggatg tcgaggacgg cgatcgggtc gaccccggcg
      421 aagggctcgt cgagcaggat gaaggccggc ttggtgccga gggcgcgcgc gatctccagc
      481 cggcgccgct cgccgcccga cagcgagatc gcggagtgct tgcgcaggtg cgcgatgttg
      541 aggtccttga gcagctgggc gaggcgctcc tcgatctcgc cggactgcag gccctgcagc
      601 tcgaggatcg cctgcacgtt ctcctccacc gtcaggcggc ggaacaccga gttctcctgc
      661 ggcaggtagg agatgcccat gcgggcgcgg cggtggatcg gcaggtgggt gaggtcctgc
      721 gtgtcgaggt ggatcgagcc ggcgtcggcc ggcaccagcc cgacgatcat gtagaagcag
      781 gtggtcttgc cggcgccgtt ggggccgagc agcccgacca cctcgccgct cgacacctcg
      841 agcgacacgt cggcgaccac ggtgcgcgac ttgtagcgct tgcgcaggct ctgcgcgttg
      901 agctggctca tttcttcgga gccggcgtag ccgccggctt gggctggatg atcatcttca
      961 cccgctggtc gccgtccttg ccggcgctca ccgagaagaa ctcggaggtc tggtccacca
     1021 ggatgtagtt gcccgccacc tggtcgccgc cgcggttgac gcgcgcgttc tcgaacagct
     1081 tcaccgtcga ggcgcgctcg tcgatctcga tgcgcagcgc ctcgccgtcg agccacacgc
     1141 cctccttctc cggcggccgc gggtcctggc gctggcggaa gcgcaccggc ttgccggtgg
     1201 cggtggcgta ctgatagccc tcggcgtcct gccgcaccac gatgcgctcg gcgagcagcc
     1261 gcatggtccc tttcaccagg accacgttgc cgtcgaagat gttgaggcgg cgcacctcgt
     1321 cggcgctcat gcggttggcc tcgagctcgg tgggcttttc cttgtccgct ttctcggcct
     1381 gcgcggaaag ggaaaacacc agcagaaaaa cccagaagaa cttcattgcg gcgcggactt
     1441 cgcctcgaag cggccgcgca cgtcggcgtt cagcgtgaag tgctgcgtct cgttgctgta
     1501 ctccatgccc ttgccggaaa gcgcgcgcgt ctcctccacg atgttcaccg ggcggtcggt
     1561 gcgcaccagc gagcggtcgc gcaccacctg caggtagtcc gtggtgagcc gagccgcggt
     1621 gtgcccggcg ccggcttcgc gctcgagcac cacgttgtcg tagaggtaga cgtcctcgcc
     1681 gtcgcgcgac agcttgccgc ggtcggcgcg cagcgtcatg cgcggctggt cgggcttggt
     1741 ctgcaccacg cgcggcgcgg tgagctcggt ggtgtcgtcg tccgggaagt gctccatgcg
     1801 gctcgccgac agggtgctct ccgcggcgcc gtcgcggttg taggtggtgg tggtgaagtt
     1861 caccacgatg tagtcggggt cgtgccggcg catcgcgggg tgcgtgtcct cgtcgttcac
     1921 ggcgcggtcg aggtagaagg tgagagcggc gagcgcgagc atcagtccga gcgggaacag
     1981 gcgcgtcgtc ggcactctca cgccgtgcgc accatcaaat cgcacagctc gcgcacggcg
     2041 ccgcgacccg ccggggcctc ggtcacatag tgcacgcggc tgcgcacagc ttcgggcgcc
     2101 tcgcgcggcg cgcaggcgaa gccgcagcgc tcgagcaccg gcaggtcaac cagctcgtcg
     2161 cccatgaagc cggtcgactc gggcttcagg cgttctttct tcagcagctt ttcaaaaaca
     2221 gacagcttgt catccacacc ctgaaagacg cgcttgatgc ccagctcgcg cgcacgcacc
     2281 gcgacggcct tcgagcggcg gccggagagg agcgccaccg cgacgccggc cgcagcgagc
     2341 agtttgatgc cgtgcccgtc gtgcgccgag aacgccttga gcgcctcgcc ggcagggccg
     2401 taccagagcg tgccgtcggt gagcacgccg tcgacatcga agatcatcag gcgaacgccc
     2461 cgggcccttc gcagaacctc ttgcgcgacc tctttcaaat caccttggca cggaacagat
     2521 cgtgcatgtt gagcgcgccg accagctcgc cgccggcgtc gaccaccagc agctggttga
     2581 ccttgtgcgc ctccatcagc tgcaccgcct cgaccgccag cgcgtcggcg cggatcaccc
     2641 gcgggccggt ggtcatcaca tcggcgacgc gcgcggcggc gaagtcggcg cccttctcga
     2701 gcgcgcggcg caggtcgccg tcggtgaaga tgccgccgac ccggccggcg gcgtcgcgca
     2761 ccgccgtcat gccgatgccg ccgcgggtca tctcgcgcag cgcttccttg agcggcgcgc
     2821 tcgccgggac caccggcagg cgctcgccct tgcgcatgac gtcggacacg tgcagcagcg
     2881 cctggcgcgg cagccggccg cgcggatggc tgcgtgcgaa atcgtccttg gtgaagccgc
     2941 gcgcatcgag cagcgccacg gcgagcgcgt cgccgagggc gagcgcggcg gtggtgctcg
     3001 ccgtcggcgc gaggttgagc gggcaggcct cctgcgccac cgctgcgtcg agatgcacgt
     3061 cggcatcgcg cgccagcgcg gaattctcgt tgcccgtgat ggcgatcagc ttggcgccgc
     3121 gccgcttgac cagcggcacg atgtccagga gctcttcgct tcctcctgag tacgagatgg
     3181 cgatgaacac gtcgcccgag tcgaccatgc cgagatcgcc gtggctcgcc tcggccgggt
     3241 gcacgaaata ggccggcgtg ccggtgctcg ccagggtcga ggcgatcttg cgcgcgatgt
     3301 ggccc
//
LOCUS       DASYJA010000352         3534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1231274, whole genome shotgun sequence.
ACCESSION   DASYJA010000352 DASYJA010000000
VERSION     DASYJA010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231274"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VGO02_07745"
     CDS             complement(<1..904)
                     /locus_tag="VGO02_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEV7477613.1"
                     /translation="MPRPLPGSIDETQALLASGEYVADRSLATSLFLSLAMKRPLFLE
                     GEAGVGKTEIAKVVAQALGRELIRLQCYEGLDISQAAYEWNYSRQMIEIRLAEAAGEK
                     SKDRLAADIYSEKFLIKRPLLKALEGHSPVLLIDELDRTDEAFEAFLLEVLADFQVTI
                     PELGTIKAAHPPIVIITSNRTREIHDALKRRCLYHWVGYPNAERELKILRAKVPGIAK
                     KLSEQVVAFVQALRKEDLFKSPGVAETLDWATALTELDAVALDPATVSDTLGVLLKYQ
                     DDIARLDGGKVKELLDQVQAELRAA"
     gene            928..1914
                     /locus_tag="VGO02_07750"
     CDS             928..1914
                     /locus_tag="VGO02_07750"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7477614.1"
                     /translation="MTSRRAFLCAALTLASARLVAQEKPAARVYRIGVLDPVSQQANG
                     ANMEQFLKGLKDLGYTQGGNLTIEYLSADGRSEQFAKLAGDLVRSQVELIVTNGTPAT
                     LAAKNASAGKVPVVTATVIDPVETKVVDSLERPGGNVTGVAIETADLEAKRLDLLRAI
                     APGRSRIAVIMDMSNPASAETWKAMQPAAKAQAVELELIDARTADDVARALAKAVQLK
                     AGAATVRLGALTPGSRRTIVEAAAQHKLPTMYASRQFVESGGLVSYGLNTPQMYYRAA
                     AFVDKILKGAKPADLPMERPTRFELVLNRLAAHRLGLVIPPDLFLKSDQVVG"
     gene            complement(1919..2716)
                     /locus_tag="VGO02_07755"
     CDS             complement(1919..2716)
                     /locus_tag="VGO02_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEV7477615.1"
                     /translation="MHAFHYHRPASAKDAVSSIGKNSEAKYLAGGQSLVQAMKLRLSS
                     PSDLVDLGTIKELSGIKVSGGSVEIGAMTRHADVAASADVKKAIPALAALAGMIGDRQ
                     VRNMGTIGGSLANNDPAADYPAAALGLGATITTNKRKIEADKFFTGLYETALEPGELV
                     TSVSFPAPKRAAYMKFKNPASRFAIVGVFVADFGGKVRVGVTGAGPSVFRQAEMEKAL
                     SAKFAPESVANIKVKAEGLNNDLHASPEYRAHLITVMAKRAVEAALK"
     gene            complement(2726..>3534)
                     /locus_tag="VGO02_07760"
     CDS             complement(2726..>3534)
                     /locus_tag="VGO02_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV7477616.1"
                     /translation="QVVADRLGMAIENVSIVHGDTSKVLFGMGTYGSRSIAVGGTAIV
                     KALDKVIAKGRKIAAHLMEAAESDVEWDRGTYKVAGTDKKKTFGEVAFAAYVPHNYPH
                     DKLEPGLNENAFYDPANFTFPAGTYVCEVEIDRDTGVVKIERFTAVDDFGKIINPMIV
                     EGQVHGGLAQGIGQALLENSIYDKEGQLVTGSFMDYCIPRASDVPMFQVDTRETPCTH
                     NPLGVKGCGEAGAIGSPAALMNAITDALGVKDLAMPATAQTVWRSLQKAA"
BASE COUNT          540 a         1184 c         1181 g          629 t
ORIGIN      
        1 ccgcggcccg cagctccgcc tgcacctgat cgagcagctc cttgaccttg ccgccgtcga
       61 gccgcgcgat gtcgtcctgg tatttcagca gcacgcccag tgtgtcggac actgtcgccg
      121 gatcgagcgc gacggcgtcg agttcggtca acgcggtcgc ccagtcaagc gtctcggcaa
      181 cgcccggtga cttgaacagg tcttccttgc gcagcgcctg cacgaaggcg accacctgtt
      241 cggacaattt cttggcgatg cccggcactt tggcgcgcaa gattttcagc tcgcgctcgg
      301 cattcggata gccgacccag tgatagaggc agcgccgctt gagcgcgtcg tggatttcgc
      361 gcgtccggtt ggaggtgatg atgacgatcg gcgggtgcgc ggctttgatg gtgccaagct
      421 ccgggatcgt cacctggaaa tccgccagca cctccaagag gaaggcctcg aaggcctcgt
      481 cggtgcggtc gagttcgtcg atcagcagca ccggcgaatg gccttcgagc gctttcagca
      541 gcggccgctt gatcaggaat ttctccgagt agatgtcggc ggccagccgg tccttcgact
      601 tctcgcccgc cgcctcggcc agccggatct cgatcatctg ccgcgagtag ttccactcgt
      661 aggccgcctg cgagatatcg aggccctcgt agcactgcag ccggatcagc tcgcggccga
      721 gcgcctgcgc caccaccttg gcgatctcgg tcttgcccac ccccgcctcg ccctccagga
      781 acagcggccg cttcatggcc agggacagga aaagggaggt ggcgaggctg cggtcggcca
      841 cgtactcgcc cgaggcgagc agggcctgcg tttcgtcgat cgatccaggg agcgggcgcg
      901 gcatggaaag gattatagtg gcgtcaaatg acgtcccggc gggccttcct ctgcgcggcg
      961 ctcacccttg cctcggcccg gcttgtggcg caggaaaagc ccgcggcgcg ggtctaccgc
     1021 atcggcgtgc tcgacccggt gtcgcagcag gccaacggcg ccaacatgga gcagttcctc
     1081 aagggcctga aggacctcgg ttacacccag ggcggcaacc tcaccatcga atacctgtcg
     1141 gcggatggcc gcagcgagca gttcgcgaag ctcgccggcg acctggttcg cagccaggtc
     1201 gaactgatcg tgaccaacgg cacgcctgcg acgctcgcgg cgaagaacgc ctcggcgggc
     1261 aaggttcccg tcgtcacggc gacggtcatc gacccggtgg agacgaaggt ggtggacagc
     1321 ctggagcgac ccggcggcaa cgtcaccggc gtagccatcg agaccgccga cctggaggcg
     1381 aagcgcctcg acctgctgcg cgccatcgcc cccgggcgct cgcgcatcgc cgtcatcatg
     1441 gacatgagca accccgcgtc cgccgagacg tggaaggcga tgcagccggc ggccaaggcg
     1501 caggcggtgg agctcgagct gatcgacgcc cgcacggccg acgatgttgc gcgcgcgctc
     1561 gccaaggccg tgcagctcaa ggccggcgcg gccacggtgc gcctcggcgc gctcacgccc
     1621 ggcagccggc gcaccatcgt cgaagccgcg gcccagcata agctgcccac gatgtatgcc
     1681 tcgcgccagt tcgtcgagtc gggcgggctg gtttcctacg gtctgaacac tccgcagatg
     1741 tactaccgcg ccgcggcgtt cgtcgacaag atcctgaaag gcgcgaagcc ggccgacctg
     1801 ccgatggagc ggccgacgcg cttcgagctg gtgctcaacc gcctcgcggc gcaccggctc
     1861 ggtctcgtta tcccgcccga tctgttcctc aagtccgacc aggtggtcgg ctagctacct
     1921 actttagtgc cgcctcgacg gcgcgcttgg ccattaccgt gatcaggtgc gcgcggtact
     1981 cggggctggc gtgcaggtcg ttgttcagcc cttcggcttt gaccttgatg ttggccaccg
     2041 actcgggcgc gaacttggcg gacagggctt tttccatctc ggcctggcgg aacacgctgg
     2101 gtccggcgcc ggtgacgccg acgcgcactt tgccgccgaa atccgccacg aacacgccga
     2161 cgatggcgaa gcgcgacgcc gggttcttga acttcatgta cgcggcgcgc ttgggcgcgg
     2221 ggaagctgac cgaggtgacg agctcgcccg gctcgagagc cgtctcgtac aggccggtga
     2281 agaacttgtc ggcctcgatc ttgcgcttgt tggtggtgat ggtcgcgccc aggccgagcg
     2341 ccgcggccgg gtagtcggcc gccgggtcgt tgttggcgag cgagccgccg atggtgccca
     2401 tgttgcgcac ctgccggtcg ccgatcatgc cggcgagggc ggcgagcgcg gggatcgcct
     2461 tcttgacgtc ggccgatgcc gcgacgtcgg cgtgacgcgt catcgcgccg atctccacgg
     2521 agccgcctga caccttgatt cccgaaagct ccttgatggt gcccagatcc accaggtccg
     2581 agggcgacga gagccgcagt ttcatcgcct ggaccaggct ctggcctccc gccaggtatt
     2641 tggcttccga gtttttgccg attgaagaaa ccgcgtcctt cgcgctcgcg gggcggtggt
     2701 agtggaatgc gtgcatgtcg ttctcctaag cggctttttg cagcgagcgc caaacggtct
     2761 gcgcggtggc gggcatcgcc aggtccttca cgccgagcgc gtcggtgatc gcgttcatga
     2821 gcgcggcggg cgagccgatg gcgccggctt cgccgcagcc cttcacgccg agcgggttgt
     2881 gggtgcaggg cgtctcgcgc gtgtcgacct ggaacatcgg cacgtcggac gcccgcggga
     2941 tgcagtaatc catgaacgat ccggtcacca attgcccttc cttgtcatag attgaatttt
     3001 ccagcagcgc ttgcccgatg ccctgcgcga ggccgccgtg cacctgcccc tcgacgatca
     3061 tcgggttgat gatcttgccg aagtcgtcga ccgcggtgaa gcgctcgatc ttcacgacgc
     3121 cggtgtcgcg gtcgatctcg acctcgcaga cgtaggtgcc ggccgggaag gtgaagttcg
     3181 ccggatcgta gaaggcgttt tcgttgagcc cgggctcgag cttgtcgtgc gggtagttgt
     3241 gcggcacgta ggccgcgaac gccacctcgc cgaaggtctt cttcttgtcg gtgccggcca
     3301 ccttgtaggt gccgcggtcc cactcgacgt cgctctccgc cgcctccatc aggtgcgcgg
     3361 cgatcttcct gcccttggcg atgaccttgt cgagcgcctt gacgatggcg gtgccgccca
     3421 cggcgatcga gcgcgagccg taggtgccca tgccgaacag gaccttcgag gtatcgccat
     3481 gcacgatcga gacgttctcg atcgccatgc cgagccgatc cgccaccacc tgcg
//
LOCUS       DASYJA010000353         2504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2048346, whole genome shotgun sequence.
ACCESSION   DASYJA010000353 DASYJA010000000
VERSION     DASYJA010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2048346"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="VGO02_07765"
     CDS             complement(<1..865)
                     /locus_tag="VGO02_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEV7477617.1"
                     /translation="MAQPQPAAHQVLVRVRAAGLNRGEFIAGHGLTAPGAAKPAGQEA
                     AGEVLGSGERVMGRAPGAFAEYALMDRREAIPIPAGMSFEQAASIPLTFMVVHDMLVE
                     QGGLKAGEWLLVTGVSSGVGVAALQAAKALGAKVIGTSGSAEKLDRLKPLGLDLALQT
                     RKDFSKNALEATAGLGVNLIVNTVGGSVFAEAQKSLAFQGRMAIVGYVDGVLKGEIDL
                     DAVHAKRLRIFGVSNKLRNAEQRAATVRGFTADFLPLIAAGRIAPLVDKVFAFGELAA
                     AKAYMEADKHVG"
     gene            complement(919..1872)
                     /locus_tag="VGO02_07770"
     CDS             complement(919..1872)
                     /locus_tag="VGO02_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7477618.1"
                     /translation="MRFVLAVVALSFSCFSTGQTFPSKPITMVVGFEPGGGTDTVARV
                     VAKFLADNVGQQVVVENRAGAGGNIAVDYTIKSAPDGYTLVLANVGSLTVNPHLMKLG
                     YDPLTDLAPVTMAVVFPNIIVTHPKANARTLAEFVKRAREKPNTVTYGSSGVGGAGHL
                     AGALLEQMAHIQLVHVPYKGGGPAMRGLVGGEIDAYISTPVAAMPHIQSGRVFAIAST
                     GAKRSTALPNVPTIAESGYPGYEAVNWYAYLVPAKTPRPIVEQLNRELGKVLRTPAAV
                     KLLENQGVDPEPTTPEALGAYMKREYETWGKVVKQADIKAE"
     gene            complement(1874..>2504)
                     /locus_tag="VGO02_07775"
     CDS             complement(1874..>2504)
                     /locus_tag="VGO02_07775"
                     /inference="COORDINATES: protein motif:HMM:NF004781.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV7477619.1"
                     /translation="IVLAGAGDKAFVSGADISQFEKERSSADAVQRYEELAEGAAAKL
                     QGCDKPLIAMIRGYCLGGGMNIAVLCDLRIAADDARFGIPAAKMGLGYRASSMKNLVD
                     VVGAAYAREIMITARQFTAAEAKEMGLVNKVVAVSELEKAVAEYTQMIAGNAPLTMRT
                     AKRIIREIGAVNYDAAKCKAWAKECFESADYKEGRKAFMEKRKPVFTGK"
BASE COUNT          371 a          864 c          822 g          447 t
ORIGIN      
        1 tgccgacgtg cttgtcggct tccatgtagg ccttcgccgc ggcgagctcg ccgaacgcaa
       61 acaccttgtc gacgagcggc gcgatgcggc cggcggcgat caaggggagg aagtcggcgg
      121 tgaagccgcg caccgtcgcc gcgcgctgct cggcgttgcg cagcttgttc gacacgccga
      181 agatgcgcag ccgcttcgcg tgcaccgcgt cgaggtcgat ctcgcctttg aggacgccat
      241 cgacgtagcc gacgattgcc atgcgtccct ggaacgcgag cgatttctgc gcttctgcga
      301 acaccgagcc gcccacggta ttcactatca ggttgactcc caaccctgcg gtcgcttcaa
      361 gcgcgttttt cgaaaaatct tttcttgttt gaagcgcgag atcgaggcca agcggcttga
      421 ggcggtcgag cttctccgcg gagccggaag tgccgatgac tttcgcgccg agcgccttgg
      481 cggcctgcag cgcggcgacg ccgacgcccg acgagacgcc ggtgaccagc agccactcgc
      541 cggctttcag gccgccctgc tccaccagca tgtcgtgcac caccatgaag gtgagcggga
      601 tcgacgcggc ctgctcgaag ctcatgccgg cggggatggg aatcgcctcg cgccggtcca
      661 tcagcgcgta ctcggcgaag gcgcccgggg cgcggcccat cacgcgctca ccgctgccga
      721 gcacctcgcc ggccgcctcc tggcccgcgg gcttcgccgc accaggcgcc gtcaggccgt
      781 ggccggcgat gaactcgccg cgattcagcc ctgcggcgcg cacgcgcacg aggacctggt
      841 gcgcggcggg ctgcggctgc gccacctccc gcagttcaag cctggtttct tttttcaaag
      901 actctagaaa gtaggctttc actcggcctt gatgtcggct tgcttgacga ccttgcccca
      961 ggtctcgtac tcgcgcttca tgtacgcgcc gagcgcctcc ggcgtggtcg gctccgggtc
     1021 gacgccctgg ttctcgagga gcttcacggc cgcgggcgtg cgcagcacct tgcccagctc
     1081 gcggttgagc tgctcgacga tcgggcgcgg cgtcttcgcc ggcaccaggt aggcgtacca
     1141 gttcaccgcc tcgtagccgg gatagcccga ctcggcgatg gtcggcacgt tgggcagcgc
     1201 ggtggagcgc ttcgcgccgg tgctcgcgat ggcgaacacc cggccgctct ggatatgcgg
     1261 catggccgcg accggcgtcg agatgtaggc gtcgatctcg ccgcccacca gcccgcgcat
     1321 cgccggcccg ccgcccttgt acggcacgtg caccagctgg atgtgcgcca tctgctcgag
     1381 cagcgcgccg gccaggtgcc cggcgccgcc cacgcccgag gagccgtaag tgacggtatt
     1441 gggcttctcc cttgcgcgct tgacgaactc ggccagcgtt cttgcgttgg ctttcggatg
     1501 cgtgacgatg atgttgggga acaccaccgc catggtcacc ggcgcgaggt cggtcagcgg
     1561 atcgtagccg agcttcatca ggtgcggatt caccgtcagg gagccgacat tggcgagcac
     1621 cagggtgtag ccgtcgggcg cgctcttgat cgtgtagtcg accgcgatgt tgccgccggc
     1681 gccggcgcgg ttctcgacca ccacctgctg gccgacgttg tcggcgagga acttggcgac
     1741 gacccgcgcc acggtgtcgg tgccgccgcc gggctcgaag cccaccacca tggtgatcgg
     1801 cttggagggg aaagtctgcc cggttgaaaa acaggagaac gacaacgcga caacggcaag
     1861 gacgaatctc atctcacttt cccgtgaata cgggcttgcg tttctccatg aaggccttgc
     1921 gtccctcctt gtagtccgcg ctctcgaaac actccttcgc ccatgccttg cacttggcgg
     1981 cgtcgtagtt cacggctccg atctcgcgga tgatgcgctt cgcggtgcgc atggtgagcg
     2041 gcgcgttgcc cgcaatcatc tgggtgtact cggcaaccgc cttttccagc tcggagacgg
     2101 caacaacctt gttgaccaat cccatctcct tagcctcggc tgcggtgaac tggcgcgcgg
     2161 tgatcatgat ctcgcgcgcg taggcggcgc cgacgacgtc caccaggttc ttcatcgacg
     2221 aggcgcggta acccaggccc atcttcgccg ccgggatgcc gaagcgcgcg tcgtcggcgg
     2281 cgatgcgcag gtcgcacagc accgcgatgt tcatgccgcc gccgaggcag tagccgcgga
     2341 tcatcgcgat cagcggcttg tcgcagccct gcagcttcgc cgcggcgccc tcggcgagct
     2401 cctcgtagcg ctgcaccgcg tcggcgctcg agcgctcctt ctcgaactgc gagatgtccg
     2461 cgcccgagac gaacgccttg tcgcctgccc cggcgaggac gatg
//
LOCUS       DASYJA010000354         3252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2138904, whole genome shotgun sequence.
ACCESSION   DASYJA010000354 DASYJA010000000
VERSION     DASYJA010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2138904"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VGO02_07780"
     CDS             <1..238
                     /locus_tag="VGO02_07780"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEV7477620.1"
                     /translation="IVDSKGISTQLYRIAGQEKLKTPIGEFDTLKLVRSRDNESAEIW
                     LASQLGNFPVRVVVVYKDGKRLEQMAVRVSVPAP"
     gene            235..1479
                     /locus_tag="VGO02_07785"
     CDS             235..1479
                     /locus_tag="VGO02_07785"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEV7477621.1"
                     /translation="MRLTRGLFRHAETALTGLLQFSGPADEQLSRYFREHRELGQQDR
                     AFVAETVFAVLRRKRSLEAAAGSAEPHALLAAALMRLQGLSARALEGLVDSSLAAGIR
                     GARAELPIAVRADLPDWLWERLAAGYGEAEALRIAQGMLNPAPLDLRVNLARTGREAV
                     LERLARDGIDAAPTVHSPAGVRLAAKPAINRHELFREGLIEVQDEGSQLLAWLLAPRR
                     GEMVADYCAGAGGKTLALAMLMRGAGRLYALDVSAKRLAALAPRAARAGISNVHSIVL
                     AGDNDARARRLAGKIDRVLVDAPCSGFGTLRRNPDLKWRQGPRAIEELAAKQARILEA
                     AAPLVKAGGRLVYATCSLLREENEAIADAFAAAHPEFEALSCGELLASQRIAMEGERL
                     RLWPHRHGTDGFFAAAFARRTA"
     gene            1612..2790
                     /locus_tag="VGO02_07790"
     CDS             1612..2790
                     /locus_tag="VGO02_07790"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEV7477622.1"
                     /translation="MLFSGLLDLPWWGCVLVLLALTHITIASVTIYLHRCQAHRAVEL
                     HPIVSHFFRFWLWLTTGMVTKEWAAIHRKHHAKCETGDDPHSPQVHGINRVLWLGVFL
                     YVKESHHPETMERYGHGTPDDWVERNVYSRSSVWGLWLMGAINVALFGLVPGMLILTA
                     QIVWIPFWAAGVINGIGHFWGYRSWDVPAPDASRNIFPIGMLIGGEELHNNHHAFPAS
                     ARFSVKWYEFDLGWMYITALEALGLAIVKKVAPKPRFAQAKAAADLETLHAVIANRYD
                     VLSRYTKSVQRTYADELERLKHWSPRDAEVLRSLKRALLRGQALAGAESAKLGEALKK
                     SRALATVIAMRDELVALWERSSASKEQLLRQLQDWCHRAEASGVAPLVDFSHRLRSYA
                     "
     gene            complement(2854..3009)
                     /gene="rpmG"
                     /locus_tag="VGO02_07795"
     CDS             complement(2854..3009)
                     /gene="rpmG"
                     /locus_tag="VGO02_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV7477623.1"
                     /translation="MREKIKLESSAGTGHFYTTTKNKRTTPDKLEIKKYDPKARKHVM
                     YKEGKLK"
     gene            complement(3020..>3252)
                     /gene="rpmB"
                     /locus_tag="VGO02_07800"
     CDS             complement(3020..>3252)
                     /gene="rpmB"
                     /locus_tag="VGO02_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV7477624.1"
                     /translation="QVTGKKPMKGHLVSHANNKTRRLFQPNLHYRRFWVESQSRWVRL
                     RVSTAGLRRIDKLGVEAVMAEIKAKAKSAAKA"
BASE COUNT          500 a         1081 c         1081 g          590 t
ORIGIN      
        1 catcgtggac agcaagggga tttccaccca gctctaccgc atcgccgggc aggaaaaact
       61 caagaccccg ataggggagt tcgacacgct caagctggtg cgctcgcgcg acaacgaatc
      121 cgccgagatc tggctcgcca gccaattggg caatttcccg gtgcgcgtgg tggtcgtgta
      181 caaggacggc aagcgcctgg agcagatggc ggtgcgggtt tcggttcccg ctccgtgagg
      241 ctgacccggg ggctgttccg gcacgcggaa acggcgctga ccggcctgct gcaattctcc
      301 ggtcccgcgg acgagcagct gtcgcgctac tttcgcgagc accgcgagct cggccagcag
      361 gaccgcgcat tcgtcgcgga gactgtgttc gcggtgctgc gccgcaagcg ttcccttgag
      421 gcggcggcgg gttccgccga gccgcacgcc ctgctcgccg ccgcgctgat gcgcctgcag
      481 ggcttgtcgg cgcgcgctct ggaaggcctg gttgattcct ctctggcggc cggcatccgc
      541 ggcgcgcgcg cagagttgcc gatagcggtg cgcgccgatc ttccggactg gctgtgggag
      601 cggctcgcgg cgggttatgg cgaggcggaa gcgctgcgca tcgcgcaggg catgctcaat
      661 ccggcgccgc tcgatttgcg cgtcaacctc gcgcgtaccg ggcgcgaagc cgtcctggaa
      721 cgcctggccc gcgacggcat cgacgccgcg cctaccgtgc actcccccgc aggagtccgc
      781 ctcgcggcca agccagccat caatcgccac gagttgtttc gcgaggggct gatcgaagtc
      841 caggacgagg gcagtcagct gctcgcctgg ctgctcgcgc cgcgccgcgg cgagatggtc
      901 gccgactact gcgccggggc aggcggcaag acgcttgccc tcgccatgtt gatgcgcggc
      961 gccgggcgcc tctatgcgct cgacgtctcg gcgaagcgtc tagccgcgct cgcgccgcgc
     1021 gcggcgcgcg ccggtatctc caatgtgcat tcgatcgtgc tcgcgggaga caacgacgcc
     1081 cgcgcccgac gcctggcggg aaagatcgac cgcgtgctgg tcgacgcgcc ctgcagcggc
     1141 ttcggcacct tgcggcgcaa cccggacctg aagtggcggc agggtcctcg cgcgatcgag
     1201 gagctcgccg cgaagcaagc gcgcatcctg gaggccgcgg cgccgctggt gaaggcgggc
     1261 ggccgcctgg tctacgccac ctgcagcctg ctgcgcgaag aaaacgaggc gatcgccgac
     1321 gcgttcgctg ccgcgcaccc ggagttcgag gcgctctcct gcggcgagct gctggcatcc
     1381 cagcgcatcg cgatggaagg cgagcggctg cgactgtggc cgcaccggca cggcaccgac
     1441 ggttttttcg ccgccgcctt tgcacggcgt accgcctgaa cgaatcccgg ttcgatatgt
     1501 cctagaaaag ttcctttttg aacaatgctc aggacaaagt tcacgacccg gccggagcat
     1561 ttcgcgtaga atggccgctg cctcttataa cgattactaa ggcaccctct gatgctattt
     1621 tcaggcctgc tcgatctccc ttggtggggc tgcgtgctcg tcctgctggc tctgacgcac
     1681 atcaccatcg cgtcggtgac catctacctg catcgctgcc aggcgcaccg cgcagtagag
     1741 ctgcatccga tcgtctccca tttcttccgc ttctggctct ggctcaccac cgggatggtg
     1801 accaaggagt gggccgcgat tcaccgcaag caccacgcga agtgcgagac cggagacgat
     1861 ccgcacagcc cgcaggtcca tggcatcaac cgcgtgctgt ggctgggcgt gttcctctat
     1921 gtgaaggagt cgcaccaccc ggaaacgatg gaacgctacg gtcacggcac gcccgatgac
     1981 tgggtcgagc gcaacgtcta ctcgcgtagc tcggtgtggg ggctgtggct gatgggcgcg
     2041 atcaacgttg cgctgttcgg cctggttccc ggcatgctga tcctgaccgc gcagatcgtc
     2101 tggatcccct tctgggcggc aggcgtgatc aacggcatcg gccatttctg gggctatcgc
     2161 agctgggacg tgccggcgcc cgatgccagc cgcaacattt tcccgatcgg catgctgatc
     2221 ggcggcgaag agctgcacaa caatcaccac gcattccctg cctccgcccg cttctcggtc
     2281 aagtggtatg agttcgatct cggctggatg tacatcacgg cgctcgaggc tctcggcctc
     2341 gccatcgtga agaaggtcgc gcccaagccg cgcttcgcgc aggccaaggc ggccgccgac
     2401 ctcgagacgc tgcatgcagt gatcgccaac cgctacgacg tgctttcccg ctacaccaaa
     2461 tcggtacaac gtacctacgc cgacgagctg gaacggctga agcactggtc gccgcgcgac
     2521 gcggaagtgc tgcgctcgct caagcgcgcg ctgctgcgcg gccaggcgct ggccggcgcg
     2581 gaaagcgcga agctcggcga agcgctgaag aagtcgcgcg cgctcgccac ggttatcgcg
     2641 atgcgcgacg agctggtcgc gctctgggag cgatcgagcg cgtcgaagga gcagctgctg
     2701 cgccagttgc aggactggtg ccaccgcgcc gaggcgagcg gcgtcgcgcc gctggtcgac
     2761 ttctcgcacc gcctgcgctc ctacgcgtaa acaggacccc gcaaaagaaa aagcccgctg
     2821 acgcgggctt ttttttcgag tgcgggtttt gcgttacttc agcttgcctt ccttgtacat
     2881 cacgtgcttg cgcgccttcg gatcgtactt cttgatctcc agcttgtcgg gcgtagtgcg
     2941 cttgttcttg gtggtggtgt agaagtggcc ggtgccggcc gacgattcaa gcttgatctt
     3001 ttcgcgcatg gcgttctcct caggccttgg ccgcggactt ggccttggct ttgatctcgg
     3061 ccatcacggc ctcgacgccg agcttgtcga tgcggcgcag gccggcggtc gacacgcgca
     3121 ggcgcaccca gcggctctgg ctctctaccc agaagcgccg gtagtgcagg ttgggctgga
     3181 acaggcgccg cgtcttgttg ttggcgtgcg agacgaggtg gcccttcatc ggcttcttgc
     3241 cggtcacttg gc
//
LOCUS       DASYJA010000355         1540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2957515, whole genome shotgun sequence.
ACCESSION   DASYJA010000355 DASYJA010000000
VERSION     DASYJA010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2957515"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="VGO02_07805"
     CDS             <1..1235
                     /locus_tag="VGO02_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007581951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HEV7477625.1"
                     /translation="RVEEHQFKTEGQVLQSAGWLAVYGRTAGEDNQNLPAIEPSEKVP
                     VLEVSAQANQTKPPPRYTEATLLSAMEGAGKLVEDDELRAAMEAKGLGTPATRAATIE
                     GLIREEYLNRDGRELVPTPKAFSLLFALHMLHIVELASPELTGEWEHKLKLIEAGKLK
                     RDEFMKEISELVRKVVTVIKTGEIPDVVYATVPAKCPKCGGVVQENYRKFQCQKCDFS
                     LWKVTSGREWSPEEVAELITKRVIGPLTGFRSRMGKPFAAVMRLTDDLRLEFDFGQAS
                     GEGEEAPDFSGQEPLGACPKDGGRVFEHGMAYTCENAVGPKKSCDFRSGRMILQQPIE
                     RDQMKKLLETGRTELLTGFVSKKGRKFKAFLVKQPDGKIGFEFQPRAPKEKAEKPEKA
                     AEKPATRRGSAPRARSTR"
     gene            complement(1192..>1540)
                     /gene="gyrB"
                     /locus_tag="VGO02_07810"
     CDS             complement(1192..>1540)
                     /gene="gyrB"
                     /locus_tag="VGO02_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232946.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HEV7477626.1"
                     /translation="IRQTAEMLRGLIGTGAYVQRGDKKQPVRDFSEAMKWLLGEVEKA
                     TTLQRYKGLGEMNSEQLWETTMDPAMRRLLRVQIEDGIAADEIFTKLMGDEVEPRRAF
                     IELNALGVRNLDV"
BASE COUNT          291 a          504 c          520 g          225 t
ORIGIN      
        1 cgcgcgtcga ggagcaccag ttcaagaccg agggacaggt gctgcagagc gcgggctggc
       61 tggcggtgta cggacgcacg gcgggcgaag acaaccagaa cctgccggcg atcgagccga
      121 gcgaaaaagt gccggtgctg gaagtgagtg cgcaggcaaa ccagaccaag cccccgccgc
      181 gctataccga agcgacgctg ctctccgcga tggagggcgc cggcaagctg gtggaggacg
      241 acgagctgcg cgccgccatg gaagcgaagg gcctgggcac gccggcgaca cgcgccgcca
      301 ccatcgaagg cctgatccgc gaggagtacc tcaaccgcga cgggcgtgag ctggtgccca
      361 cgcccaaggc gttttcgctc ctcttcgcgc tgcacatgct gcacatcgtc gaactcgcct
      421 cgcccgagct caccggcgag tgggagcaca agctgaagct gatcgaagcc ggcaagctga
      481 agcgcgacga gttcatgaag gagatctccg agttggtgcg caaggtggtc acggtcatca
      541 agaccggcga gatccccgac gtggtctacg ccaccgtgcc ggccaagtgc cccaagtgcg
      601 gcggcgtggt gcaggagaac taccgcaagt tccagtgcca gaagtgcgac ttctcgctct
      661 ggaaagtcac ctcggggcgg gaatggtcgc ccgaggaagt cgccgagctc atcaccaagc
      721 gggtgatcgg cccccttacc ggcttccgca gccgcatggg caagcccttc gccgcggtga
      781 tgcgcctcac cgacgacctg cgcctcgagt tcgacttcgg ccaggcttca ggcgagggcg
      841 aggaggcgcc ggacttcagc ggccaggagc cgctcggcgc gtgccccaag gacggcggcc
      901 gggtgttcga gcacggcatg gcctatacgt gcgaaaacgc ggtcgggccg aagaaaagct
      961 gcgatttccg ctccggccgc atgatcctgc agcagccgat cgagcgcgac cagatgaaga
     1021 agctgctcga gaccgggcgt acggagctgc taaccgggtt cgtgtcgaag aagggacgca
     1081 agttcaaggc gttcctggtg aagcagcccg acggcaagat cggcttcgag ttccagccgc
     1141 gcgcgccgaa ggagaaggca gaaaaacccg agaaggccgc ggagaagccc gctacacgtc
     1201 gaggttccgc accccgagcg cgttcaactc gatgaacgcg cggcgcggct cgacctcgtc
     1261 gcccatcagc ttggtgaaga tctcgtcggc ggcgatgccg tcctcgatct gcaccctgag
     1321 cagccggcgc attgcgggat ccatggtcgt ctcccagagc tgctccgagt tcatctcgcc
     1381 cagccccttg tagcgctgca gcgtggtggc cttctccacc tcgccgagca gccacttcat
     1441 cgcttcggag aagtcgcgca ccggctgttt cttgtcgccg cgctgcacgt acgcgccggt
     1501 gccgatcagg ccgcgcagca tctccgcggt ctggcggatc
//
LOCUS       DASYJA010000356         7347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_414780, whole genome shotgun sequence.
ACCESSION   DASYJA010000356 DASYJA010000000
VERSION     DASYJA010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7347
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414780"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /locus_tag="VGO02_07815"
     CDS             complement(<1..1350)
                     /locus_tag="VGO02_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEV7477627.1"
                     /translation="MAHDAAPSGRLRGNEWRALGRLAPYLLEYKWRVGIALAFLVVAK
                     LANVAVPLIMKQVVDGLDPRLQALAVPVALLAIYGALRFSATLFAELRDVVFVRVAQR
                     AIRRVALGVFRHLHSLSLRFHLERQTGGMTRDIERGTRGISTLLSYLLFSIIPVILEF
                     TLVAAVLLGKFDWRFAAITFGAVAVYIAFTVAITEWRMEIRRRANDLDSRANTRAIDS
                     LLNYETVKYFGNEEFEARRYDENLKKYEDAATRSEASLGLLNIGQSLIIALALTALMV
                     LAAGGVVGGGLTLGDLVLVNGLLIQLYIPLNFLGMVYREIKQSLLDMDKMFRLLSENR
                     EVEDKPGAAPLAVRGGSVRFENVEFSYDGRREILRNVSFEVPAGKKVAVVGTSGSGKS
                     TLARLLFRFYDVGKGRITIDGQDLRDVTQESVRAALGVVPQDTVLFNDTILYNISYGR
                     "
     gene            complement(1363..2424)
                     /locus_tag="VGO02_07820"
     CDS             complement(1363..2424)
                     /locus_tag="VGO02_07820"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV7477628.1"
                     /translation="MTERADAAVGCLLGAAVGDARGLPWEGLSRRRQKRMAQYMGESL
                     IAGRDMCSDDTDHACMVAQAIAVSAGSPRRFVSSLAWRLRFWLAGLPAGIGLGTLVAI
                     LKLWLFIPPQFSGVRSAGNGPAMRSAILGVAFSESDPLLVMHVSASSAITHSDPRAQF
                     GALAVALAARSAMRGEIPEEYLFHLERFAAPFGDAGKELVDLAQRAAASAAAGEQTVA
                     FAASIGCERAVSGYIYHSVPVALHAWLRNPGNLETAIEAVLACGGDTDTVAAITGGIV
                     GAGVGRAGIPESHLARLWDWPRGARWIEDAARNALAAADTRIPRAQLYLSPFKLLGRN
                     LVLLGVVLAHGFRRLLPPY"
     gene            complement(2408..2725)
                     /locus_tag="VGO02_07825"
     CDS             complement(2408..2725)
                     /locus_tag="VGO02_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477629.1"
                     /translation="MTGGSMVGLVLLSMAWLFFAGGAVTNFSVLRMARRAKEGERLPS
                     GIPFIPGVAGSLAAFFTLPALAQYGIDVPWPWLWILLPLFLDVYCLGWIFFLSRTKND
                     RAG"
     gene            2795..3664
                     /locus_tag="VGO02_07830"
     CDS             2795..3664
                     /locus_tag="VGO02_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEV7477630.1"
                     /translation="MGLRLKFNLVLLTVFVVGLGVTGYVSYELLHRNARDEVLRNAGV
                     MMEASLSMRSYTVGQVRPNLAVDPDKFLPQSVPAFGATEIMTLLRKKYPDYAYKEAAL
                     NPTNPRNRAVEWETDIVNSFRANPTQGEISGTRETPTGRSLYLARPFQIKDPACLSCH
                     TNADMAPVAMVKIYGPNNGFGWQLNEVIGAQIVSVPMDLPIRNANHAFVTFMSSLTVV
                     FAVLFVILNAMLSAMIVQPITQLSRAADQISKGRLDAAEFKSTGKDEVSQLGQAFNRM
                     RRSLEKAISLIEK"
     gene            3681..5063
                     /locus_tag="VGO02_07835"
     CDS             3681..5063
                     /locus_tag="VGO02_07835"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV7477631.1"
                     /translation="MSAQALPAGYGLLEYRIEKVLGVGGFGLTYLATDANLNLKVALK
                     EYLPGDIALRGADQTIAPRSPDTAESFGWGKQRFLDESRTLASFRHPNIVRVMRFFEA
                     KGSAYMVMEFVDGAALGEWVKARRPIAEAQAAAIVAPLLDGLEVVHKSGFLHRDIKPG
                     NIYVREDGSPVLLDFGSARQQSAELTAVVTPGYAPFEQYHTQGNQGPWSDLYALAGVL
                     YWMVTGNPPAEAAARVREDKMPGALTVGDHARFRADFLRAIDWALMPFEDKRPQSVAE
                     WRARLLSSPSKGVSAEPKTEKLAPQPQSALVFDPALLKRLETALAQHAGPIAPLVVRN
                     AAKKARAQPELIQLVAGEIGDPATRAAFEKSFSEDSRPPSQPSGGPRTAREPSSALAA
                     SRLSPEALERAERRLAEYIGGISRVLVKRAALKAHTEAELYLLLADEIADKEEKKAFM
                     RKGLSKSGRS"
     gene            5113..5808
                     /locus_tag="VGO02_07840"
     CDS             5113..5808
                     /locus_tag="VGO02_07840"
                     /inference="COORDINATES: protein motif:HMM:NF037008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7477632.1"
                     /translation="MNPPWSPAMPLRAVKPPHETRTRILDAAEELFMQHGFEATSMRL
                     LTTRAEVNLAAVNYHFGSKHALIEAVFRRRLDPMNAARVAALEQLEADGRATDPERII
                     RAFVGESLRMIEDTRSGGRNFIRLLGRTYTEPAKPIRALIGQLYVPVMARFKAAFERA
                     LPHMPREEVVWRMHFMFGTLSYTLAATDAVQLIAGFKPEDRHDAHLLEDRLTAFLLAG
                     LAAPLKTAVKKAA"
     gene            5818..>7347
                     /locus_tag="VGO02_07845"
     CDS             5818..>7347
                     /locus_tag="VGO02_07845"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEV7477633.1"
                     /translation="MAGLGLALGALAVFLLFAYFQLPLIWWSVAAGLLAWGLSALGDF
                     SFVSNIFLAAACVVVAAVLNLPFLRRILITNNILAVYRRILPDMSQTEKEAIDAGTVW
                     WDGDLFSGKPDWDKLLAVPAPRLSAEEQAFLDGPVEELCAMCNDWEITHERQDLPPRV
                     WQFIKDKGFLGMIIPKKFGGLGFSALAHSAVVMKLSTRSAATAITVMVPNSLGPGELL
                     LHYGTDEQKDHYLPRLAKGLEVPCFALTNPEAGSDAAAIPDRGVVCKGIWQGREVLGM
                     RVTWDKRYITLGPVATLLGLAFRLYDPEHLLGEKEDLGITCALVPTNTSGVNIGRRHL
                     PLNAAFQNGPNWGKDVFMPLDWIIGGRDFAGKGWMMLMGCLAAGRAISLPTSAVGGTK
                     ALTRFTGAYARVRAQFKTPIGRLVGVEEALGRIAAHTYMMDATRVMTAGAVDLGEKPA
                     VLSAIAKYHMTERARACVNDAMDIHGGKGICLGPNNWIGRGYQIAPVAITVEGANILT
                     RT"
BASE COUNT         1249 a         2556 c         2445 g         1097 t
ORIGIN      
        1 cctgccatat gaaatgttgt acaggatcgt gtcgttgaag agcacggtgt cctggggcac
       61 gacgccgaga gccgcgcgca cgctctcctg ggtgacatcg cgcaggtcct ggccgtcgat
      121 ggtgatgcgg cccttgccga cgtcgtagaa acggaacagc agccgcgcga gcgtggactt
      181 gcccgagccg ctcgtgccga ccaccgcgac cttcttcccc gccggcacct cgaagctcac
      241 gttcctcagg atctcgcgcc ggccgtcgta gctgaattcc acgttctcga agcgcacgga
      301 gccgccgcgc acggcgagcg gcgccgctcc gggcttgtcc tcgacttcgc ggttctcgga
      361 gagcagccgg aacatcttgt ccatatcgag cagcgactgc ttgatctcgc ggtacaccat
      421 gccgaggaag ttgagcggga tgtagagctg gatcagcagc ccgttcacca gcaccagatc
      481 gccgagcgtc aggccgcccc ccaccacgcc gccggcggcc agcaccatca gcgccgtcag
      541 cgccagcgcg atgatcaggc tctggccgat gttgagcagg ccgagcgagg cttcgctgcg
      601 cgtcgccgcg tcctcgtact tcttcaggtt ctcgtcgtag cgccgcgcct cgaattcctc
      661 gttgccgaag tacttcaccg tctcgtagtt gagcaggctg tcgatggcgc gcgtgttggc
      721 gcgcgaatcc aggtcgttgg cgcgccggcg gatctccatg cgccactcgg tgatcgccac
      781 ggtgaaggcg atgtagacgg cgacggcgcc gaaggtgatg gcggcgaagc gccagtcgaa
      841 cttgccgagc agcaccgcgg cgaccagggt gaactcgagg atcaccggga tgatcgagaa
      901 cagcagatag gacagcagcg tcgagatccc gcgcgtgccg cgctcgatgt cgcgcgtcat
      961 gccgccggtc tgccgctcga ggtggaagcg caggctcaag ctgtgcaggt ggcggaacac
     1021 gccgagcgcc acgcgccgga tcgcccgctg cgcgacgcgc acgaacacca cgtcgcgcag
     1081 ctcggcgaac agcgtcgccg agaagcgcag cgcgccgtag atcgcgagca gcgccaccgg
     1141 caccgcgagg gcctgcaggc gcgggtccag gccgtcgacc acctgcttca tgatcagcgg
     1201 cactgcgacg ttggcgagct tcgcgaccac caggaaggcg agggcgatgc cgacgcgcca
     1261 cttgtattcc aggaggtagg gcgcaagcct gccgagcgcg cgccactcgt tgcctctcaa
     1321 ccgcccgctg ggcgctgcgt catgggccat cgcccaatta tctcaatacg gcggcagcag
     1381 cctgcgaaac ccgtgggcga gaacgacccc gaggaggacg aggtttctgc ccagcagctt
     1441 gaaaggcgag aggtagagct gcgcgcgcgg gattcgcgtg tcggcggcgg cgagcgcatt
     1501 gcgcgccgcg tcctcgatcc agcgcgcgcc gcgcggccag tcccagaggc gcgccaggtg
     1561 cgactcgggt attccggcgc gtccaacccc tgcgccgacg atgccgccgg tgatcgccgc
     1621 gacggtatcg gtgtcgccgc cgcaggcgag caccgcctcg atcgcggtct ccaggttgcc
     1681 ggggttcctg agccaggcgt gcagcgccac cgggacgctg tggtagatgt agccgctcac
     1741 cgcgcgctcg cagccgatcg atgccgcgaa ggccacggtc tgctcgcccg ccgcggcgct
     1801 cgccgccgcg cgctgcgcga gatcgaccag ctccttgccg gcgtcgccga agggcgcggc
     1861 gaagcgctcg aggtggaaca ggtactcctc ggggatctcg ccgcgcatcg ccgagcgcgc
     1921 ggcgagcgcc accgccagcg cgccgaactg cgcgcgcggg tcgctgtggg tgatcgccga
     1981 gctggccgag acgtgcatca ccagcagcgg gtcgctttcc gaaaaagcca cgccgaggat
     2041 ggcgctgcgc atcgccgggc cgttgccggc cgagcgcacg ccggagaact gcggcgggat
     2101 gaacagccag agcttgagga tggcgacgag cgtgcccagg ccgatgccgg ccggcaggcc
     2161 cgccagccag aagcgcagcc gccaggcgag gctggagacg aagcggcgcg ggctgcccgc
     2221 cgacaccgcg atcgcctgcg ccaccatgca ggcgtggtcg gtgtcgtccg agcacatgtc
     2281 gcgcccggcg atcagcgact cacccatgta ctgggccatg cgcttttgcc gccggcgcga
     2341 cagcccttcc cacggcaggc cgcgcgcgtc gcccaccgcg gcgccgagga ggcagccgac
     2401 ggcggcgtca gcccgctctg tcattttttg ttctcgagag gaaaaaaatc cagcccaggc
     2461 aatacacgtc gaggaacagg ggcagcagga tccacagcca cggccagggc acgtcgatgc
     2521 cgtattgcgc gagggccgga agtgtaaaaa atgcagccaa tgagccggct acgcccggaa
     2581 tgaaggggat gccggaaggc agccgctcgc cttccttcgc ccgccgcgcc atgcgcagga
     2641 ccgagaaatt cgtcacggcg ccgccggcga agaacagcca ggccatggaa agcagaacca
     2701 gtccaaccat ggagccgccg gtcatcggat cagtgtaagc tcatccagtg ggcagcgact
     2761 cacgtacagg gagtgggaac acaaggggag aatcatggga ctgcgactga aattcaacct
     2821 ggtgctgctg accgttttcg tggtcgggct gggcgtcacc ggatacgttt cgtacgagct
     2881 tctgcaccgc aacgcgcgcg acgaggtgct gcgcaatgcc ggcgtaatga tggaagcctc
     2941 gctttccatg cgcagctaca ccgtcgggca ggtgcggccc aacctcgcgg tcgaccccga
     3001 caagttcctg ccgcagagcg tgccggcgtt cggcgccact gagatcatga cgctgctgcg
     3061 caagaagtac cccgactacg cctacaagga agcggctctc aacccgacca acccgcgcaa
     3121 ccgcgccgtc gagtgggaga ccgacatcgt caactccttc cgcgccaacc cgacgcaggg
     3181 cgagatctcg ggcacgcgcg agacgcccac cgggcgctcg ctgtacctgg cgcggccgtt
     3241 ccagatcaag gacccggcct gcctgtcctg ccacaccaac gcggacatgg cgccggtggc
     3301 gatggtcaag atctacggcc cgaacaacgg cttcggctgg cagctgaacg aggtgatcgg
     3361 cgcgcagatc gtctcggtgc cgatggacct gccgatccgc aacgccaacc acgccttcgt
     3421 caccttcatg agctcgctca cggtggtgtt cgcggtgctg ttcgtgatcc tcaacgccat
     3481 gctgagcgcg atgatcgtgc agccgatcac gcagctgtcg cgcgccgccg accagatctc
     3541 caagggccgc ctggacgccg ccgagttcaa gagcaccggc aaggacgagg tgtcgcagct
     3601 cggccaggcc ttcaaccgca tgcggcgcag cctcgagaag gcgatcagcc tgatcgaaaa
     3661 atagtccgta ggggtttttc atgtccgcgc aggccctccc ggctggctac ggcctgctgg
     3721 aataccgcat cgagaaagtg ctcggcgtcg gcggcttcgg cctgacctac ctcgccaccg
     3781 acgccaacct caacctcaag gtggcgctga aggaatacct gcccggcgac atcgcgctgc
     3841 gcggcgccga ccagaccatc gcgccgcgct cgcccgacac ggcggagagc ttcggctggg
     3901 gcaagcagcg cttcctcgac gagtcgcgca ccctcgcctc gttccgccac ccgaacatcg
     3961 tgcgcgtgat gcgcttcttc gaggcgaagg gctcggccta catggtgatg gagttcgtcg
     4021 acggggcggc gctcggcgaa tgggtcaagg cgcgccgccc gatcgccgag gcgcaggcgg
     4081 cggcgatcgt cgcgccgctg ctcgacgggc tcgaggtggt gcacaagtcg ggcttcctgc
     4141 accgcgacat caagccgggc aacatctacg tgcgcgagga cggcagcccg gtgctgctcg
     4201 acttcggctc ggcgcgccag caatccgccg agctcaccgc ggtggtgacg ccgggctacg
     4261 cgccgttcga gcagtaccac acgcagggca accagggacc gtggagcgac ctgtacgcgc
     4321 tcgccggggt gctgtactgg atggtgaccg gcaatccgcc ggccgaggcc gccgcgcgcg
     4381 tgcgcgagga caagatgccc ggcgcgctca ccgtcggcga ccatgcgcgc ttccgcgcgg
     4441 acttcctcag ggcgatcgac tgggcgctga tgcccttcga ggacaagcgg ccgcagtcgg
     4501 tggccgaatg gcgcgccagg ctgctttctt ctccttcgaa gggagtttct gcagagccga
     4561 aaaccgaaaa gctcgcgccc cagccccaat cggccctggt gttcgacccg gcgctgctca
     4621 agcggctgga gaccgcgctc gcgcagcacg ccggcccgat cgcgccgctg gtggtgagga
     4681 acgcggcgaa gaaagccagg gcgcagcccg agctgatcca gctggtcgcc ggcgagatcg
     4741 gcgacccggc gacgcgcgca gcgttcgaga agtcgttttc ggaagactcg cgcccgccct
     4801 cgcagccgtc gggcggaccg agaaccgcgc gcgagccgtc gagcgccctc gccgccagcc
     4861 gcttgtcgcc cgaagcgctc gagcgggcgg agcggcggct cgccgagtac atcggcggca
     4921 tttcgcgcgt gctggtcaag cgcgccgccc tgaaggcgca taccgaagcc gagctctacc
     4981 tcctgctcgc cgacgagatc gcggacaagg aggagaagaa ggctttcatg cgcaaagggc
     5041 tttcgaagtc cggaagatcc tgaattccgc ttgacgcggc ggcacggccg cggctagcat
     5101 tcaaacgttc gtttgaatcc gccctggagc cccgccatgc ccctgcgcgc cgtcaagccg
     5161 ccgcacgaaa cccgcacccg catcctcgac gccgccgagg agctgttcat gcagcacggc
     5221 ttcgaggcca cctcgatgcg cctgctcacg acgcgcgccg aagtcaacct cgccgcggtc
     5281 aactaccact tcggctcgaa gcacgcgctg atcgaggcag tcttccgccg gcggctcgac
     5341 ccgatgaacg cggcgcgcgt cgccgcgctc gagcagctcg aggccgacgg gcgcgccacc
     5401 gaccccgagc gcatcatccg cgccttcgtc ggcgagagcc tgcgcatgat cgaggacacc
     5461 cggagcggcg ggcgcaactt catccgcctg ctcggccgca cctacaccga gccggccaag
     5521 ccgatccgcg cgctgatcgg gcagctgtac gtgccggtga tggcgcgctt caaggcggcc
     5581 ttcgagcgcg cgctgccgca catgccgcgc gaggaagtcg tctggcgcat gcacttcatg
     5641 ttcggcacgc tgtcctacac gctcgccgcc accgacgcgg tgcagctcat cgccggcttc
     5701 aagcccgagg accgccacga cgcgcacctg ctcgaggatc ggctcaccgc cttcctcctc
     5761 gccggcctcg ccgcaccgct gaaaaccgcc gtcaagaaag ccgcctagga gatcgccatg
     5821 gccggactcg gactcgcact cggtgcgctc gcagtctttc ttctgttcgc ctactttcaa
     5881 ttgccgctca tctggtggag cgtcgcagcc ggcctgctcg cctggggcct gagcgcgcta
     5941 ggcgattttt cctttgtttc caatattttt ctggcagcgg catgcgtggt cgttgccgcc
     6001 gttctcaacc tgccgttcct tcgcaggatt ctcatcacca acaacatcct ggccgtctac
     6061 cgccgcatcc ttcccgacat gtcgcagacc gagaaggagg cgatcgacgc cggcacggtg
     6121 tggtgggacg gcgatctgtt ctcgggcaag cccgactggg acaagctgct cgcggtgccg
     6181 gcgccgcgcc tgagcgccga ggagcaggcg ttcctcgacg gcccggtcga ggagctgtgc
     6241 gcgatgtgca acgactggga gatcacccac gagcgccagg acctgccgcc gcgcgtgtgg
     6301 cagttcatca aggacaaggg cttcctcggc atgatcatcc cgaagaagtt cggcggcctg
     6361 ggcttcagcg cgctggcgca ttcggcggtg gtgatgaagc tctcgacgcg ctccgcggcg
     6421 acggcgatca cggtgatggt gcccaactcg ctcgggccgg gcgagctgct gctgcactac
     6481 ggcaccgatg agcagaagga ccattacctg ccgcgcctcg ccaagggcct cgaagtgccc
     6541 tgcttcgcgc tcaccaatcc cgaggccgga tccgacgcgg cggcgatccc cgaccgcggc
     6601 gtggtgtgca agggcatctg gcagggcagg gaagtgctcg gcatgcgcgt cacctgggac
     6661 aagcgctaca tcacgctcgg gccagtggcg acgctcctcg gtcttgcttt tcgcctttac
     6721 gatcctgaac acctcctggg agaaaaggaa gatctcggca tcacctgtgc gctggtgccg
     6781 accaatacct cgggggtgaa catcgggcgg cgccatctgc cgttgaatgc ggccttccag
     6841 aacgggccga actggggcaa ggacgtgttc atgccgctcg actggatcat cggtggccgc
     6901 gacttcgccg gcaaaggctg gatgatgctg atgggctgcc tcgccgcggg ccgcgccatc
     6961 tcgctgccca cctccgccgt aggcggcacg aaagccttga cgcgcttcac cggcgcgtac
     7021 gcacgcgtgc gcgcgcagtt caagacgccg atcggccgcc tggtaggcgt cgaggaagcc
     7081 ctcggccgca tcgccgcgca cacctacatg atggacgcta cgcgggtcat gaccgccggc
     7141 gcggtcgacc tcggcgagaa accggcagta ctctcggcga tcgccaagta ccacatgacc
     7201 gagcgagcgc gcgcctgcgt caacgacgcc atggacatcc acggcggcaa gggcatctgc
     7261 ctcgggccga acaactggat cggccgcggc taccagatcg cgccggtcgc catcacggtg
     7321 gaaggcgcga acatcctgac gcgcacg
//
LOCUS       DASYJA010000357         5050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1413525, whole genome shotgun sequence.
ACCESSION   DASYJA010000357 DASYJA010000000
VERSION     DASYJA010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413525"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="VGO02_07850"
     CDS             complement(<1..149)
                     /locus_tag="VGO02_07850"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515026.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEV7477634.1"
                     /translation="MTNDPIVIVAAARTPMGAFQGELKSFAAPELGAAAIRAAVERAK
                     IKPEDV"
     gene            complement(151..753)
                     /gene="can"
                     /locus_tag="VGO02_07855"
     CDS             complement(151..753)
                     /gene="can"
                     /locus_tag="VGO02_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HEV7477635.1"
                     /translation="MSLDQLLQNNRAWAKEMLSQDKFFFSRLAQQQLPQYLWIGCSDS
                     RVPANQITGLAPGEIFVHRNIANVVVHTDLNCLSVIQFAVDVLRVRHIILCGHYGCSG
                     VHAAMTKHRIGLSDNWLRHVQDVAGKHDSELRTLADENLRHRRLCEMNAREQVQNIVQ
                     CTAVQEAWERGQPLEVHGWIYDLTDGLLRDLQVSQSEPRS"
     gene            complement(750..1895)
                     /locus_tag="VGO02_07860"
     CDS             complement(750..1895)
                     /locus_tag="VGO02_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEV7477636.1"
                     /translation="MNYLKFDHGEAVAMLRDSVRDFAVKEIAPRAAQIDASNQFPPDL
                     WRKMGSLGLLGITVEEEYGGSAIGYLAHIVAMEEISRASASIGLSYGAHSNLCVNQIR
                     RNGSPAQKKKYLPKLVSGEHVGALAMSEPGAGSDVVGMKLKAERRGERYMLNGNKMWI
                     TNGPDADVLVIYGKTSDQGISAFLVEKGMKGFSTAQKLDKLGMRGSNTCELVFRDCEI
                     SKDQILGEEGKGVKVLMSDLDYERAVLAGGPLGIMAACLDVVVPYVHERKQFGQPIGE
                     FQLMQGKLADMYTTFSACRAYVYAVGQALDRNETTRKDAAGAILYAAEKATWMAGEAI
                     QALGGMGYINETPTGRLWRDAKLYEIGAGTSEIRRWLIGRELFDETA"
     gene            complement(1892..2329)
                     /locus_tag="VGO02_07865"
     CDS             complement(1892..2329)
                     /locus_tag="VGO02_07865"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003274768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV7477637.1"
                     /translation="MNAPADPHFAARVRESFGRQKAMGLIGAALGAVEPGYVEVALPF
                     REDLTQQKGFIHGGILGMIADTACGYSAFSLMPADCSLVTVEYKINILTPARGSLLAK
                     GQVVKAGRTLTVARAEVYSEDGKHVATMQQTLMMLAGSPDTIE"
     gene            complement(2366..2746)
                     /locus_tag="VGO02_07870"
     CDS             complement(2366..2746)
                     /locus_tag="VGO02_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV7477638.1"
                     /translation="METERAEFSISELAREFAITPRAIRFYEDQGLLAPRREGQRRIY
                     TPRDRTRLKLTLRGKRLGLTLSEIRELIDMYEPGRDERPQLERFLAVLESHRASLLQQ
                     RADIEAQLAEIQGFEKKIRKRLRS"
     gene            2770..3804
                     /locus_tag="VGO02_07875"
     CDS             2770..3804
                     /locus_tag="VGO02_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7477639.1"
                     /translation="MSSLVKSSLEYPFAEVPAPGATLEVAPGVRWLSMPLPFQLDHIN
                     LWLLEDGDGSTVVDTGIGNAQTRALWEQILTRKVKRVILTHYHPDHAGNAAWLLARDG
                     APMWTTQGEYLTAHAVRSSSAGYTADAVLAVFRANGLDAERLKGMAEMRGNNRYAELV
                     PEFPHSYRRIIDADRVRIGEHEWTAIIGHGHAPEHLSLFSKELNTLIAGDMLLSTIST
                     NVSVWSIDPEGDPLRLFLDSIARYRELPQDVLVLPSHGRPFRGAHTRVAELERHHQEK
                     FEILSGVLRARPQSAGELLGVLFPKRKLDLHQTFFAMGEAIAHLHYLYYAGRATREPG
                     DDGIMRYATR"
     gene            3791..>5050
                     /locus_tag="VGO02_07880"
     CDS             3791..>5050
                     /locus_tag="VGO02_07880"
                     /inference="COORDINATES: protein motif:HMM:NF018827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEV7477640.1"
                     /translation="MQPANQDEQKMLQAVAERSSRILGEFAQKQAESLSSAMRDELGI
                     AKAFMDLYSHAAMDPSVLASASVNWWIDSMHLWQSSWMKMLGGQAQPVAAPEKGDARF
                     KDQDWSANFVFDYIKQSYLIAARHTRAAVAGVEGLSEESEKKVAFFTRQYVDALAPTN
                     FLMTNPQVLRETLASGGKNLVKGLNNLLADLEKGHGELRVSMTDESAFQLGKNVATTP
                     GKVVFQTDLMQLIQYLPTTPDVYKRPLVIIPPWINKYYILDLREKNSFIRFALEQGHT
                     VFVLSWVNPDARLAQKGFDDYMIEGPLAALDYIEKATGESKVNFIGYCLGGTLLGAAL
                     GYLQEKKQDRVACATFFVSLLDFSQPGELGVFIDEAQVANLERKMNQRGYLEGSEMAG
                     TFNLLRANDLVWSFVVNNYLMGKDPFPF"
BASE COUNT          863 a         1632 c         1625 g          930 t
ORIGIN      
        1 acgtcctcgg gcttgatctt cgcgcgctcc acggcggcgc ggatcgccgc ggcgccgagc
       61 tcgggcgcgg caaagctttt tagctcgccc tggaaggcgc ccataggcgt gcgggcggcg
      121 gcaacgatga cgataggatc gttggtcatg tcagctcctt ggttcggact gcgacacctg
      181 aagatcgcgc agcaggccat cggtgagatc ataaatccag ccgtgcactt cgagcggctg
      241 gccgcgctcc cacgcttcct gcaccgcggt gcactgcacg atgttctgca cctgctcgcg
      301 cgcgttcatt tcgcacaggc gccggtgccg cagattctcg tcggccagcg tcctgagctc
      361 ggagtcgtgc tttccggcga cgtcctgcac gtggcgcagc cagttgtcgg acaggccgat
      421 gcggtgcttg gtcatcgccg cgtgcacgcc gctgcagccg tagtggccgc acaggatgat
      481 gtggcggacc ctgaggacgt cgacggcgaa ctggatcacc gacaggcaat tgaggtcggt
      541 gtgcaccacg acgttggcga tgttgcggtg gacgaagatc tcgccggggg cgaggccggt
      601 gatctggttg gcgggcacgc gcgagtcgga gcagccgatc cagaggtact gcggcaattg
      661 ctgctgcgcc agccggctga aaaaaaactt gtcctgagaa agcatttcct ttgcccaggc
      721 gcggttgttc tgcaggagct ggtccaggct catgccgtct cgtcgaacag ctcgcggccg
      781 atcagccagc ggcggatctc cgaagtgccg gcgccgatct cgtagagctt ggcgtcgcgc
      841 cacagtcgcc cggtcggcgt ctcgttgata tagcccatgc cgccgagcgc ctggatcgcc
      901 tcgcccgcca tccaggtggc cttctcggcg gcgtacagga tcgcgccggc ggcatctttc
      961 ctggtcgttt catttctatc caaagcttga ccaaccgcgt acacgtacgc acgacaagcg
     1021 gagaaagtcg tgtacatgtc ggcgagcttg ccctgcatga gctggaactc gccgatcggc
     1081 tggccgaact gcttgcgctc gtggacgtag ggcaccacca cgtcgaggca ggcggccatg
     1141 atgccgagcg gcccgccggc aagaaccgcg cgctcgtagt ccagatcgga catcagcacc
     1201 ttcacgccct tgccttcctc gcccaatatc tggtcttttg atatttcaca gtccctgaag
     1261 acgagctcgc aggtgttcga cccgcgcatg ccgagcttgt cgagcttctg cgcggtggag
     1321 aatcccttca tgcccttttc gaccaggaag gcgctgatgc cctgatcgct tgtcttgccg
     1381 tagatcacca gcacatcggc gtcggggccg ttggtaatcc acatcttgtt gccgttcagc
     1441 atgtagcgct cgccgcgcct ttcggctttc aacttcatcc cgaccacgtc cgagccggcg
     1501 ccgggctcgg acatggcgag cgcgccgacg tgctcgcccg acacgagctt cggcaggtac
     1561 tttttcttct gcgcaggcga gccgttgcgg cggatctggt tgacgcacag gttggagtgg
     1621 gcgccgtagg aaaggccgat cgacgccgag gcgcgcgaga tctcctccat cgccacgatg
     1681 tgggcaaggt agccgatggc gctgccgccg tattcctcct ccacggtgat gccgagcagg
     1741 cccaggctgc ccatcttgcg ccacaggtcc gggggaaact ggttggaggc gtcgatctgc
     1801 gcggcgcgcg gcgcgatttc cttcaccgcg aaatcgcgca ccgagtcgcg cagcatggcg
     1861 acggcttcgc cgtggtcgaa tttcaggtag ttcattcgat ggtgtccgga gaaccggcga
     1921 gcatcatcag cgtctgctgc atggtcgcca cgtgcttgcc gtcctcagag tacacctcgg
     1981 cgcgcgccac ggtcagcgtg cgcccggctt tcaccacctg gcccttggcg agcagcgagc
     2041 cgcgcgccgg cgtgaggatg ttgatcttgt actcgacggt caccagcgag cagtcggcgg
     2101 gcatcagcga gaacgcggaa tagccgcacg ccgtgtcggc gatcatgcct aggatgccgc
     2161 catggatgaa gcccttctgc tgcgtcaggt cttcacggaa gggcagggcg acctccacgt
     2221 agccgggctc gaccgcgccc agggcggcgc cgatcagccc catcgccttc tgccgaccga
     2281 agctctcccg cacgcgggca gcgaaatgag gatcggcggg agcgttcata cgaaccggct
     2341 aggctacttt acgtttacgt aaacgtcaac ttcgcaagcg tttgcggatt ttcttttcga
     2401 agccctggat ctcggcgagc tgcgcctcga tgtcggcgcg ctgctgcagc aggcttgccc
     2461 tgtgcgactc gagcaccgcg aggaagcgct cgagctgcgg ccgctcgtcg cgccccggct
     2521 cgtacatgtc gatcagctcg cggatctcgg agagcgtgag gcccaggcgc ttgccgcgca
     2581 gcgtgagctt gaggcgggtg cggtcgcgcg gcgtgtagat gcgccgctgg ccctcgcggc
     2641 gcggcgcgag cagcccctgg tcctcgtaga agcggatcgc gcgcggcgtg atcgcgaact
     2701 cgcgtgcgag ctcgctgatg gagaattcgg cgcgctccgt ttccatggta gcttcaaaga
     2761 ttctagctga tgtcctcgct cgtgaagtcc tctctggaat atcccttcgc cgaagtcccg
     2821 gcgccgggcg cgacgctcga agtcgcaccc ggcgtgcgct ggctgtcgat gccgctgccg
     2881 tttcagctcg accatatcaa cctgtggctg ctggaggacg gcgacggctc cacggtggtg
     2941 gataccggaa tcggcaacgc tcagacgcgg gcgttgtggg aacagattct tacgcgaaaa
     3001 gtgaagcggg tgatcctcac ccactaccac cccgaccacg ccggtaacgc ggcctggctg
     3061 ctggcgcgcg acggcgcgcc gatgtggacc acgcaaggcg aatacctcac cgcgcacgcg
     3121 gtgcgctcgt cgagcgcggg ctataccgcc gacgcggtcc tggcggtgtt ccgcgccaac
     3181 ggcctggacg ccgagcgcct gaagggcatg gcggagatgc gcggcaacaa ccgctatgcc
     3241 gagctggtgc ccgagttccc gcattcctac cgccgcatca tcgacgccga ccgcgtgcgc
     3301 atcggcgagc atgagtggac ggcgatcatc ggccacggcc atgcgccgga gcatctgtct
     3361 cttttttcga aggaattgaa tacgctgatc gccggcgaca tgctgctgtc gaccatctcg
     3421 accaacgtca gcgtctggtc gatcgacccc gagggcgacc cgctgcgcct gttcctcgac
     3481 tcgatcgcgc gctatcggga acttccgcag gacgtgctgg tcttgccttc gcacggcagg
     3541 ccgttccgcg gcgcgcatac ccgcgtcgcc gagctggagc gccaccacca ggagaaattc
     3601 gagatcctgt cgggggtgct gcgcgcacgg ccgcaaagcg caggcgagct gctcggcgtg
     3661 ctgtttccga agcgcaagct cgatctgcac cagactttct tcgcgatggg cgaggcgata
     3721 gcccacctgc attatctcta ctacgccggc cgcgcaacgc gcgaaccggg cgacgatggc
     3781 atcatgcgct atgcaacccg ctaatcagga cgaacagaaa atgctgcagg cggtggccga
     3841 gcgctcatcg cgcatcctcg gcgagttcgc ccagaagcag gccgagagcc tttcctcggc
     3901 gatgcgcgac gagctgggca tcgccaaggc gttcatggat ctctactcgc acgcggcgat
     3961 ggatccgagc gtgctggcga gcgcctccgt gaactggtgg atcgactcga tgcacctgtg
     4021 gcagtcgagc tggatgaaga tgctgggcgg gcaggcgcag ccggttgcgg cgccggagaa
     4081 aggcgacgcg cgcttcaagg accaggactg gtcggccaat ttcgtgttcg actacatcaa
     4141 gcagtcctac ctcatcgccg cgcgccacac ccgcgccgcg gtagcgggcg tcgagggcct
     4201 gtccgaggaa tcggagaaga aagtcgcgtt cttcacccgg cagtatgtcg acgcgctcgc
     4261 gccgaccaac ttcctgatga ccaacccgca ggtgctgcgc gagaccctgg cgagcggcgg
     4321 gaagaacctt gtgaagggcc tcaacaacct gcttgcggac ctggagaagg gccacggcga
     4381 gctgcgcgtc tcgatgaccg acgagagcgc gttccagctc ggcaagaacg tcgccaccac
     4441 cccgggcaag gtggtgttcc agaccgacct gatgcagctc atccaatacc tgccgaccac
     4501 gccggacgta tacaagcgac cgctggtcat catcccgccc tggatcaaca agtactacat
     4561 cctcgatttg cgcgagaaga attcctttat ccgctttgcg ctcgagcagg gccacacggt
     4621 gttcgtgctc tcatgggtca accccgacgc gcgcctggcg cagaagggct tcgacgacta
     4681 catgatcgaa ggcccgttgg cggcgctcga ttacatcgaa aaggccaccg gcgagagcaa
     4741 ggtcaatttc attggctatt gccttggggg cacgcttctt ggcgctgcct tgggttattt
     4801 acaggagaaa aagcaagatc gcgtagcctg cgcgactttt ttcgtcagcc tcctcgactt
     4861 ctcccagccg ggcgagctcg gcgtgttcat cgacgaagcg caggtggcga acctcgagcg
     4921 caagatgaac cagcgcggct acctcgaggg ctcggagatg gccggcacct tcaacctgct
     4981 gcgtgccaac gacctggtgt ggtccttcgt ggtcaacaac tatctgatgg gcaaggaccc
     5041 gtttcctttc
//
LOCUS       DASYJA010000358         7354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2321614, whole genome shotgun sequence.
ACCESSION   DASYJA010000358 DASYJA010000000
VERSION     DASYJA010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7354
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2321614"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VGO02_07885"
     CDS             <1..791
                     /locus_tag="VGO02_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477641.1"
                     /translation="EVLNFGVSGFGTAQEYVMLESSAIRYRPDLVLLQFTNGNDVRDN
                     SFALDPDKNRPFYMFARDGELRIDESFAYSEDFAARTSFRGELARKLTDKVRLLQLAR
                     SLKDTSFVPKAQAGLQGVEQGLEPMVLAAPRDRLWDEAWRITEGLIAKAGDFAQRNGA
                     RFAIVTVPYAVQVHPDAKVRAALQAKLGVSDLFYPDQRIAALAKKNGLLAVTLAPEMQ
                     PLAEKGGVYFHGFDNVGLGRGHWNAAGHKAAAGIIARRLCAAGA"
     gene            complement(757..1548)
                     /locus_tag="VGO02_07890"
     CDS             complement(757..1548)
                     /locus_tag="VGO02_07890"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV7477642.1"
                     /translation="MALYGVVADIHGNREALDVALAALERRGIRKLLCLGDVVGYNAD
                     PDECAEMLRSRGALAIAGNHDLIGTGRLGFERCSNQAMYALKRTRRSLARGTAEWLAS
                     LPPNRVIGDDVVLVHGGVRDVQQYVKHTALVEQNAEWLRADFPKARLCLFGHSHEQKI
                     YEVDGAAVRELPHADRNLLNRNLVYFVNPGSVDASRKNSQKLAEFAILDTLDWSVEFL
                     RLRYDSAATEAKAAVFGYRIGRWTDRAYTLRRRLLKLRPRTTAAR"
     gene            complement(1527..2729)
                     /locus_tag="VGO02_07895"
     CDS             complement(1527..2729)
                     /locus_tag="VGO02_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477643.1"
                     /translation="MTQTRLPVVLLGGLDIVRALGLAGIPVIIASSQRRTPSMASRYC
                     AGSIELPPISERNNVIETLAGAGRDLAVKHGARIPLFYDNDDRLALVQDYREALAPHF
                     ALLLNEPALGNALLDKTAFQDLAERAGLPVPRRLGWEARAAESGPGLAKPKTRTAWDG
                     SAVRLQLFGGAGKARVFGSGREARANPLAAQLAAQLAFQEYVPGGDEAIWSFHGFAAP
                     GGEVLAGFVGRKIRTYPAFTGDSSYVRLAREPELEALGRGIAARLGLAGIFKMDFKRS
                     SASGRCYLLEINTRFNLWHHVGARNGVNLPRIAYEFLTLHKRPEQMDAATRYRWLALR
                     YDLPAYRELRERRALNLAGWLLSLAYMPKVYELFSWSDPLPFARYWTTRLRAALARRT
                     PRWLSTAS"
     gene            2840..4003
                     /locus_tag="VGO02_07900"
     CDS             2840..4003
                     /locus_tag="VGO02_07900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7477644.1"
                     /translation="MSAAVLPRAVVHARRPAGTGVVVEVRKDAAALRPLVPEWEALAA
                     EAAEPNPFYEHWMLLPALQAYGAEDLRCIAVWDNGTLGALFPMRLERSWRHLPVAALR
                     SWSHRNMLVCTPLVRAKSAEKCVAALLQSGLAPVIEFDWIFAGGHFCGALAEAAAAGG
                     LPWMVTDAYTRALLLRERDPRERFNSNMKNNLRRWQARLGAQGKLSPARLSPGDDLAA
                     WTEEFIRLEASGWKGRAGTALGCREDDRRFVAEVFAEAFRRGRLQISGLDLDGRALAR
                     HIMLTAGEGSFSFKLAYDDTHASCSPGILAEVDNVRQFMETPGLRWIDSNTARQSTGY
                     GRVWKDRHTVQRVAVGVRGAGGPAVAALPLLRLARRVFVRTRAPDGSDQPRAG"
     gene            4063..4770
                     /locus_tag="VGO02_07905"
     CDS             4063..4770
                     /locus_tag="VGO02_07905"
                     /inference="COORDINATES: protein motif:HMM:NF020463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HEV7477645.1"
                     /translation="MRIAIHQPHFLPWLGYLHRMAQVDAFVLLDHVQFERRNYQNRTM
                     IRMNGAAHWLTVPVVQRSRDERIVDKEVDNTESGERSWGKVCARTLQHIYGKAPFFRE
                     HAPALKAILDARWEKLVDVNQAGLDFLREVFGIRTPLLRSSELGVQGAKADLVLEICR
                     ATQADVLLAGMGGSRGYLSVEEFARRGVRIEWHQFQHPVYPQPAPQPFTPGLSAIDML
                     FHIGPELLQRAPQMKAA"
     gene            4767..5459
                     /locus_tag="VGO02_07910"
     CDS             4767..5459
                     /locus_tag="VGO02_07910"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEV7477646.1"
                     /translation="MNAAVLPIGRLPIARKAACTFPARYAGRTVLAVGAHPDDIELGV
                     GATVALLARAGARVVMAVCSVPGDYDRRRSEALDAAAILGCELRILMDGGSRRIEDVK
                     AYQLVGMIDDMVRELRPAAMLTHGSTDFHGDHVAVHHASVPSQRLHAFDFYCYLPTMV
                     RPVPVPFQPRAYIDVSTTIETKIDAIAAHKSQFGSRGISFDYYREIAHLNGVMCGAEY
                     AEGLDIGRIVFA"
     gene            complement(5456..5875)
                     /locus_tag="VGO02_07915"
     CDS             complement(5456..5875)
                     /locus_tag="VGO02_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010098173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEV7477647.1"
                     /translation="MGTGMAAFGELASQAARREKEAELLFVGDAYRQAIGQYYESSPA
                     GHKRYPASLEQLLKDERFAFNRRYLRRLYADPATGKAFATMQAPEGGIMGVFSTSEAA
                     PAKSGNFPPEYIAFEGAARLSEWTFLYVPGAGATASR"
     gene            complement(6081..6440)
                     /locus_tag="VGO02_07920"
     CDS             complement(6081..6440)
                     /locus_tag="VGO02_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEV7477648.1"
                     /translation="MKKRSGFTLVELMVVLTVIALLLSVVVPDYVGRMHNAEEAVLRE
                     NLTMMRDALDKHYADAGRYPAALNDLVTKRYLRSIPPDPFTRSPATWVAVPPPGQTKD
                     KGNVYDVKSGAKGYEGW"
     gene            complement(6443..6886)
                     /locus_tag="VGO02_07925"
     CDS             complement(6443..6886)
                     /locus_tag="VGO02_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEV7477649.1"
                     /translation="MKGFTLIEMLIAAAIVALLATVALPLAEVTVQRNKEQDLRRSLR
                     EVRGAIDAYKRAMDEGRIEKKVDRSGYPPSLTVLVEGVKDVRSPKDEKIFFLRRLPRD
                     PLTGQQWGLRSYASAADSPQAGDDVYDVFTRSSSVGLNGVPYKEW"
     gene            complement(6883..>7354)
                     /locus_tag="VGO02_07930"
     CDS             complement(6883..>7354)
                     /locus_tag="VGO02_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477650.1"
                     /translation="RTPAGTVSYQVGTRNAATTLRLKDGETQVLAGLINDEDRRAATQ
                     VPGLANLPLLGRLFRNNNDTVNKTEIVLLITPRVVRGIERPEARLEEFKSGTELELGG
                     AGSGGNVPQPVAPTQPSQPQPPQPVRESQPQTPYLEPGSPRPPAAGPAPQGKPQ"
BASE COUNT         1068 a         2540 c         2618 g         1128 t
ORIGIN      
        1 tcgaggtgct gaacttcggc gtctcgggct tcggcacggc gcaggaatac gtgatgctcg
       61 agtcgagcgc catccgctac cggcccgacc tggtgctgct gcagttcacc aacggcaacg
      121 acgtgcgcga caactccttc gcgctcgacc cggacaagaa ccggccgttc tacatgttcg
      181 cccgggacgg cgagctgcgc atcgacgagt cgttcgcgta ctcggaggac ttcgcggcgc
      241 gcacctcctt caggggcgag ctcgcgcgca agctgaccga caaagtccgg ctgctgcagc
      301 tggccagatc cctgaaggac acctccttcg tgccgaaagc gcaggccggg ttgcagggcg
      361 tcgagcaggg gctggagccg atggtgctcg ccgcgccgcg cgaccggctg tgggacgaag
      421 cctggcgcat caccgagggg ctgatcgcca aggccggcga cttcgcgcag cgcaacggcg
      481 cccgcttcgc catcgtcacc gtgccgtacg ccgtgcaggt gcatcccgac gccaaggtgc
      541 gcgccgccct gcaggccaag ctcggcgtga gcgatctctt ctatcccgac cagcgcatcg
      601 ccgcgttggc gaagaaaaac gggctactcg cggtgacgct ggcccccgag atgcagcccc
      661 tcgccgagaa aggcggggtg tacttccacg gctttgacaa tgtcggcctg ggccgcgggc
      721 actggaacgc cgccggccac aaggccgccg ccggcatcat cgcgcggcgg ttgtgcgcgg
      781 ccggagcttg aggagcctgc gcctcagggt gtaggcgcgg tcggtccagc gcccgatgcg
      841 gtagccgaac actgccgcct tcgcctcggt ggcggcgctg tcgtagcgca ggcgcaggaa
      901 ctcgacgctc cagtcgagcg tgtcgaggat cgcgaactcg gcgagcttct ggctgttctt
      961 gcgcgaggcg tcgaccgatc cgggattgac gaagtacacg agattcctgt tcagcagatt
     1021 cctgtccgcg tgcggcagct cgcggacggc tgcgccgtct acttcgtaga tcttctgctc
     1081 gtggctgtgg ccgaacaggc acaggcgcgc cttgggaaag tcggcgcgca gccactcggc
     1141 gttctgctcg accagcgcgg tgtgcttcac gtactgctgc acgtcgcgca cgccgccgtg
     1201 caccaggacc acgtcgtcgc cgatgacgcg gttcggcggc aggctggcga gccactcggc
     1261 ggtgccgcgc gccaggctgc ggcgcgtgcg cttgagcgcg tacatcgcct ggttggagca
     1321 gcgctcgaag cccaggcggc cggtgccgat gaggtcgtgg ttgccggcga tcgccagcgc
     1381 gccgcggctg cgcagcatct cggcgcactc gtcggggtcg gcgttgtagc cgacgacgtc
     1441 gcccaggcag aggagcttgc ggatgccgcg gcgctccagc gccgcgaggg ccacatccag
     1501 cgcctcgcgg ttgccgtgga tgtccgctac gacgccgtag agagccatcg cggggtccgg
     1561 cgcgcgaggg cggcccgcag gcgggtcgtc cagtagcgcg cgaaaggcag gggatcgctc
     1621 caggagaaga gctcgtagac cttcggcatg taggcgagag acagcagcca gcctgccagg
     1681 ttcagcgcgc ggcgctcgcg cagctcccgg taggcgggca ggtcgtacct cagggcgagc
     1741 cagcggtagc gcgtcgcagc gtccatttgt tcggggcgtt tgtgcagggt gaggaactcg
     1801 taggcgatgc gcggcaggtt gacgccgttg cgggcgccga cgtggtgcca caggttgaag
     1861 cgggtgttga tctcgagcag gtagcagcgg ccgctggcgc tgctgcgctt gaagtccatc
     1921 ttgaagatgc ccgccaggcc gaggcgcgcg gcgataccgc ggccgagcgc ttcgagctcc
     1981 ggctcgcggg cgagccgcac gtaggagctg tcgccggtga aggcggggta agtgcggatc
     2041 ttgcgcccga cgaatccggc cagcacctcg ccgccgggcg cggcgaagcc gtggaacgac
     2101 cagatggcct cgtcgccgcc gggcacgtac tcctggaacg cgagctgcgc ggcgagctgc
     2161 gcggcgagcg ggttggcgcg cgcctcgcgg ccgctgccga agacgcgcgc cttgccggcg
     2221 ccgccgaaga gctgcaggcg cacggccgag ccgtcccacg cggtgcgcgt cttgggcttg
     2281 gcgagccccg gcccgctctc ggccgcgcgc gcctcccagc cgaggcggcg cggcaccggc
     2341 agcccggcgc gctcggcgag gtcctggaac gcggtcttgt cgagcagcgc gttgcccagc
     2401 gccggctcgt tcaggagcag cgcgaaatgc ggcgcgagcg cctcgcggta gtcctgcacc
     2461 agcgcgagcc ggtcgtcgtt gtcgtagaag agcgggatgc gtgcgccatg cttgaccgcc
     2521 agatcccggc cggcgcctgc cagggtctct attacattgt ttctttcgga aatcggcggc
     2581 aactcgatgc tcccggcgca atagcgcgag gccatggagg gcgtgcgccg ctgggacgag
     2641 gcgatgatca ccgggatccc cgccaggccg agcgcgcgca cgatgtcgag cccaccgagc
     2701 aggacgaccg gcagtcgggt ctgcgtcatc gcttcccgag gctagcaacg cagcgctgca
     2761 ttccggggcg cggcgcttga gccgtccggc gcaggcgcgc cgcgcgacgc gggacgccag
     2821 ggctttgtaa ggtagtcgaa tgtcggccgc cgttctcccc agggccgtcg tccacgcgcg
     2881 ccgtcccgca ggaaccgggg tcgtcgtcga ggtgcgcaag gacgcggccg cgttgcgccc
     2941 gctcgtgccc gagtgggaag cgctcgccgc cgaggccgcc gagcccaacc ccttctacga
     3001 gcactggatg ctgctgcccg cgctgcaggc ctatggcgcc gaggacttgc gctgcatcgc
     3061 ggtctgggac aacggcacgc tcggcgcgct gttcccgatg cgcctggagc gcagctggcg
     3121 gcacctgccg gtcgccgcgc tgcgctcctg gagccaccgc aacatgctcg tctgcacgcc
     3181 gctggtgcgc gcaaaaagcg ccgagaaatg cgtcgccgcg ctgctgcaaa gcggtctcgc
     3241 gccggtgatc gagttcgact ggatctttgc cggcgggcat ttctgcggcg ccctggccga
     3301 ggccgcggcg gccggcggat tgccgtggat ggtgaccgac gcctacacgc gcgcgctgct
     3361 cctgcgcgag cgcgatccgc gcgagcgctt caactcgaac atgaagaaca acctgcggcg
     3421 ctggcaggcg cggctgggcg ctcaaggcaa gctgagcccc gcgcgcctct cgccgggcga
     3481 cgacctcgcc gcgtggaccg aggagttcat ccggctcgag gcgagcggct ggaaggggcg
     3541 cgccggcacc gcgctcggct gccgcgagga cgaccggcgc ttcgtcgccg aggtcttcgc
     3601 cgaggcgttc cgccgcgggc gcctgcagat cagcggcctc gacctcgacg gccgggcgct
     3661 tgcccgccac atcatgctca cggcgggcga gggctccttc agcttcaagc tcgcctacga
     3721 cgatacccac gcgagctgct cgccggggat cctggccgaa gtcgacaacg tgcgccagtt
     3781 catggagacg ccggggctgc gctggatcga ctccaatacc gcacggcaaa gcaccggcta
     3841 cgggcgcgtc tggaaggacc ggcacacggt gcagcgcgtc gcggtcgggg tgcgcggcgc
     3901 gggagggccc gccgtggccg cgctgccgct cctgcgcctg gccaggcgcg tttttgtaag
     3961 aacgcgtgcg ccggacggct cagatcagcc gcgcgcgggg tgatcgatct gcccgggccg
     4021 cccggctacg ctaagcctag gagaaaccgg gagaattgcg ccttgagaat tgcgatccac
     4081 cagccgcatt tcctgccctg gctgggctat ctgcaccgca tggcgcaggt ggacgccttc
     4141 gtcctgctcg accacgtgca gttcgagcgc cgcaactacc agaaccgcac catgatccgc
     4201 atgaacggcg cggcccactg gctgaccgtg ccggtggtgc agcgctcgcg cgacgagcgc
     4261 atcgtcgaca aggaggtcga caacaccgag agcggcgagc gctcgtgggg caaggtctgc
     4321 gcgcgcacgc tgcagcacat ctacggcaag gcgccgtttt tccgcgagca cgcgccggcg
     4381 ctgaaggcga tcctcgatgc gcgctgggaa aagctggtgg acgtcaacca ggcggggctc
     4441 gattttctgc gcgaagtgtt cggcatccgc acgccgctgc tgcgcagctc cgagctcggc
     4501 gtgcagggcg cgaaagccga cctggtcctc gagatctgcc gcgcgacgca ggccgacgtc
     4561 ctgctcgccg gcatgggcgg ctcgcgcggc tatctgagcg tcgaagagtt cgcgcgccgc
     4621 ggcgtgcgca tcgagtggca ccagttccag catcccgtct acccgcaacc cgcgccgcag
     4681 ccgttcacgc ccgggctctc cgccatcgac atgctgttcc acatcggtcc cgaactgctg
     4741 cagcgcgctc cgcagatgaa agccgcatga acgcagccgt cttacccatt gggcggcttc
     4801 ccattgcccg caaggcggcg tgcactttcc cggcgcgcta cgccgggcgt accgtgctcg
     4861 ctgtcggcgc gcaccccgac gacatcgagc tcggcgtcgg cgccacggtc gcgctgcttg
     4921 cccgcgccgg ggcgcgcgtg gtgatggcgg tctgctcggt gccgggcgac tacgacaggc
     4981 gccgcagcga agccctggac gccgccgcga tcctcggctg cgagctgcgc atcctgatgg
     5041 atggcggcag ccggcgcatc gaggacgtca aggcctacca gctggtcggc atgatcgacg
     5101 acatggtgcg cgagctgcgg ccggcggcca tgctgacgca cggcagcacc gacttccacg
     5161 gcgaccacgt ggccgtgcac cacgcctcgg tcccgtcgca gcgcctgcac gccttcgatt
     5221 tctactgcta cctgccgacc atggtgcggc cggtgccggt gccgttccag ccgcgcgcct
     5281 acatcgacgt cagcaccacc atcgagacca agatcgatgc catcgccgcc cacaagagcc
     5341 agttcggctc ccgcggcatc agcttcgatt actaccgcga gatcgcgcac ctgaacggcg
     5401 tcatgtgcgg cgccgagtac gccgaaggcc tcgacatcgg ccgtatcgtg tttgcctagc
     5461 gggaggcggt agccccggcg cccggcacgt agaggaaggt ccactccgac aggcgcgccg
     5521 caccttcgaa cgcgatgtac tccggcggaa agttgcccga cttggccggc gccgcctccg
     5581 aggtgctgaa gacgcccatg atgccgccct ccggcgcctg catggtcgcg aacgccttgc
     5641 cggtggccgg gtcggcatac agccggcgca ggtagcggcg gttgaaggcg aagcgctcgt
     5701 ccttgagcag ctgctccagg ctcgccgggt agcgcttgtg cccggcgggg gagctctcgt
     5761 agtactgccc gatcgcctgc cggtacgcgt cgccgacgaa cagcagctcc gcctccttct
     5821 cgcgccgcgc cgcctgcgag gcgagctcgc cgaacgcggc catgccggtg cccatcgccg
     5881 cgacgatcag tagtagtcca aggtacgtga agcctcgctg cgaattccgg gtcagagcac
     5941 gatatgcttc gatcccctga caagggggtc gcgagccgga ggcgagcggg cggttggttt
     6001 ttttcgcgcg tcgaaaaaac caaagccgaa accccccggc gctgtgcgcc accccccttg
     6061 tcaggggggc aaaacccgag ctaccagccc tcatagccct tcgcaccgct cttcacatcg
     6121 tagacattgc ccttgtcctt cgtctggccg ggcggcggca ccgcgaccca ggtggcgggg
     6181 ctgcgcgtga acgggtcggg cgggatggag cgcaggtagc gcttggtcac caggtcgttg
     6241 agcgcggccg ggtagcggcc ggcgtcggca tagtgcttgt cgagcgcgtc gcgcatcatg
     6301 gtgaggttct cgcgcagcac cgcctcctcg gcgttgtgca tgcggccgac gtagtccggc
     6361 accacgacgg aaaggagcag agcgatgacg gtgagcacca ccatcagctc caccagagta
     6421 aaccctgagc gttttttcat ggttaccact ccttgtaggg cacgccgttc agcccgaccg
     6481 acgagctgcg ggtgaacacg tcgtagacgt cgtcgccggc ctgcggcgag tcggcggcgc
     6541 tggcgtagct gcgcaggccc cattgctggc cggtgagcgg gtcgcgcggc agccggcgca
     6601 ggaagaagat cttctcgtcc ttcgggctcc tgacgtcctt cacgccttcg accagcacgg
     6661 tgagggacgg cgggtagccc gagcggtcga ctttcttctc gatgcggccc tcgtccatgg
     6721 cgcgcttgta ggcgtcgatc gccccgcgca cctcgcgcag cgagcggcgc aggtcctgct
     6781 ccttgttgcg ctggaccgtg acctcggcga ggggcagcgc gaccgtggcg agcagcgcca
     6841 cgatcgccgc ggcgatcagc atctcgatga gagtaaagcc cttcattgcg gcttgccttg
     6901 cggcgccgga cctgcagccg gcggacgcgg cgagcccggc tcgaggtagg gcgtttgcgg
     6961 ctgcgactcg cgcaccggct gcgggggctg cggctgcgac ggctgcgtcg gcgcgacggg
     7021 ctgcggcacg ttgccgccgc tgccggcgcc gcccaactcg agctcggtgc ccgacttgaa
     7081 ctcctcgagg cgcgcctcgg gccgctcgat gccgcgcact acgcgcggcg tgatcagcag
     7141 gacaatctcg gtcttgttga cggtgtcgtt gttgttcctg aacaggcggc cgagcagcgg
     7201 caggttggcg aggcccggca cctgggtggc ggcgcggcgg tcctcgtcgt tgatcaggcc
     7261 ggccagcacc tgcgtctcgc cgtccttcag ccgcagcgtg gtggcggcgt tgcgcgtgcc
     7321 gacctgatag gagacggtgc ccgccggcgt gcgg
//
LOCUS       DASYJA010000359         1985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2685035, whole genome shotgun sequence.
ACCESSION   DASYJA010000359 DASYJA010000000
VERSION     DASYJA010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2685035"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1005
                     /locus_tag="VGO02_07935"
     CDS             88..1005
                     /locus_tag="VGO02_07935"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEV7477651.1"
                     /translation="MEDGTIVLVGATTENPSFELIAPLLSRARVLVFKPLDAAAVDKL
                     LARAEMIEGRTLPLDAEARQSLARMADGDGRAALTLVEEVWRAAREGEIFDAAKLQDV
                     VQRRAPIYDKKQDGHYNLISALHKSVRGSDPDAALYYLCRMLDAGEDPLYLARRVVRM
                     AVEDIGLADPQALVICNAAKDAYDFLGSPEGELAIAQAVIYIATAPKSNAAYMAYGAA
                     MRTAKEAGSLLPPKHILNAPTKLMQSEGYGDGYDYDHDAPEAFSGQDYFPDALGRQKF
                     YDPPERGFEREIRKRLEYWAKLRKERGRA"
     gene            1041..1352
                     /locus_tag="VGO02_07940"
     CDS             1041..1352
                     /locus_tag="VGO02_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477652.1"
                     /translation="MVATGMPASAAEPFDGRWAPDAQACIGERAVASPLVVNSFTLQW
                     RDAACVVRTSYRVRGAWHIGARCWGEGVVSNVPITLRMRGEQLLLDWAGAPAEELRRC
                     P"
     gene            1349..>1985
                     /locus_tag="VGO02_07945"
     CDS             1349..>1985
                     /locus_tag="VGO02_07945"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV7477653.1"
                     /translation="MSTATKPARGDATVAERVPPSTGVQTAKVSADEAGMRVDRFLEA
                     RFPGLSFSHIQRVIRKGELRVNGKRAQPKQRLEAGQAVRIPPLRLDQPKPRTPEHEAD
                     AKTRDFLKSITLHEDADVLVLNKPMGLAVQGGSGTTRHLDGMLEVLRDAQGQRPRLVH
                     RLDKDTAGCLLVAKTRFAATALARTFRTRSARKVYWALVAGVPKPRQGRIST"
BASE COUNT          333 a          668 c          682 g          302 t
ORIGIN      
        1 cggcgcgaga ccgggcaggg gacgctgctc ttcgtcgacg agatccatcg cttcaatcgc
       61 gcgcagcagg attccttcct gccggtcatg gaagacggca ccatcgtgct cgtgggcgct
      121 accacggaga atccgtcgtt cgagctgatt gcgccattgc tctcgcgtgc gcgcgtattg
      181 gtgttcaagc cgctggacgc tgccgcggtg gacaagctct tggcccgtgc cgagatgatc
      241 gagggcagaa ccctcccgct cgatgccgag gcgcgccaat cgctcgcccg catggccgac
      301 ggcgacggtc gcgcggcgct cacgctggtg gaagaagtgt ggcgggcggc gcgcgagggc
      361 gagatcttcg acgcggcgaa actgcaggac gtggtgcaac gccgcgcgcc gatctacgac
      421 aagaagcagg acggccacta caacctgatc agcgcgctgc acaaatcggt gcgcggctcg
      481 gaccccgacg ccgcgctgta ctatctctgc cgcatgctgg atgcaggcga ggacccgctc
      541 tacctcgccc ggcgcgtcgt gcgcatggca gtcgaggata tcgggctggc cgacccgcag
      601 gcgctggtca tctgcaatgc cgccaaggac gcctatgatt ttctgggctc gccggaaggc
      661 gaattggcga tcgcgcaggc cgtgatttat atcgcaaccg cgccgaaatc gaatgccgcc
      721 tatatggcct acggcgccgc catgcgtacc gccaaggagg cgggctcgct gctgccgccg
      781 aagcacatcc tcaacgcgcc gaccaagctg atgcagtccg aaggctatgg cgacggctac
      841 gattacgacc acgatgcgcc ggaggcgttt tccggccagg actatttccc cgacgcgctc
      901 ggccggcaga agttctatga tccgcccgag cgcggtttcg agcgcgagat caggaaacgg
      961 ctggaatatt gggccaagct tcggaaagag cggggccggg cgtgacagcc gcgcgagcaa
     1021 ctgcgtgggc ggccagcgtt gtggtcgcca cgggcatgcc ggcgagcgcg gccgagccgt
     1081 tcgatggccg ctgggcgccg gatgcgcagg cgtgcatcgg ggagcgcgcg gtcgcgtcgc
     1141 ccctcgtcgt caattcgttc accttgcagt ggcgcgatgc cgcctgcgtc gtgcgaacga
     1201 gctaccgggt gcgcggcgcc tggcacatcg gcgcgcgctg ctggggcgaa ggtgtggtca
     1261 gcaacgttcc gatcactttg cggatgcgtg gcgagcagct cctgctcgat tgggccggtg
     1321 cgccggcgga agaactgcgg cggtgtccat gagcacggcg accaaaccag cgcgcggcga
     1381 cgccaccgtt gctgagcgag tgccgccatc gaccggcgtg cagaccgcca aggtgagcgc
     1441 cgacgaagcc ggcatgcgcg tcgaccgctt cctcgaggcg cgcttccccg ggctttcgtt
     1501 ctcccacatc cagcgcgtga tccggaaggg cgagctgcgc gtcaacggca agcgcgcgca
     1561 gcccaagcag cgtctcgagg ccgggcaggc cgtgcgcatc ccgccgctgc ggttggatca
     1621 gcccaagcca cgcacacccg aacacgaggc ggatgccaag acccgcgatt tcctcaaatc
     1681 catcacgctc cacgaagatg ccgacgtgct ggtgctcaac aagcccatgg ggctcgcggt
     1741 gcagggcggc tcgggcacca cgcgccatct cgacggcatg ctcgaggtgc tgcgtgacgc
     1801 ccagggccag cgcccacgac tggtgcaccg gctcgacaag gacactgccg gctgcctcct
     1861 cgtggccaag acccggtttg cagccacggc gctcgccagg acgttccgca cccgatcggc
     1921 gcgaaaagtc tactgggccc tggtcgcagg cgtgcccaag ccgcggcagg gccggatctc
     1981 gacct
//
LOCUS       DASYJA010000360         2340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1232270, whole genome shotgun sequence.
ACCESSION   DASYJA010000360 DASYJA010000000
VERSION     DASYJA010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232270"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VGO02_07950"
     CDS             complement(<1..569)
                     /locus_tag="VGO02_07950"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEV7477654.1"
                     /translation="MRRTSRQVAVGDVLIGGGAPVVVQSMTNTDTEDAQSTARQVEEL
                     ARAGSELVRITVNTPEAAAQVPRIRARLDAIGCRVPLVGDFHYNGHKLLTQFPDCAQA
                     LAKYRINPGNVGKGKKHDEQFATMIECALRYAKPVRIGVNWGSLDADLLARLMDENAR
                     LSQPSDSVAVTREAVIRSALDSARYAEELG"
     gene            complement(575..1435)
                     /locus_tag="VGO02_07955"
     CDS             complement(575..1435)
                     /locus_tag="VGO02_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEV7477655.1"
                     /translation="MTDGSVGAALSAARQELGLGLPEVAQQLKFGLRQLEALEADRFD
                     QLPGGTFARGMVRNYARLLKIDPDPLLARIADRYDAPDSNQLAARFSQPVPFSDNARR
                     STLVYFGLSLVILVVVGGVAYQWQHERNAPKQMAFVKPKAEAPARKPALPAQQASIPA
                     PIAEEKKTEEKKIEEKKIEEKKVEEAKPVERKPAPSVKTVAAPPASGVHRIVLRVETD
                     EAWLEVTDGAGRQLVSSLNRAGSERVVQGRGPFTLVIGNASHVRVLHNDHELDLKPYT
                     RVEVARFTIP"
     gene            complement(1428..2207)
                     /gene="pilW"
                     /locus_tag="VGO02_07960"
     CDS             complement(1428..2207)
                     /gene="pilW"
                     /locus_tag="VGO02_07960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEV7477656.1"
                     /translation="MRRALFLAAAALLAGCAAQPVEGPSVETGQVTGESGDPRNRARL
                     HTELAGLYYSRGNMNVALEELRAAVAADSNYATAYGLFGLVYMDLKENSLAQQNFDRA
                     LRLAPNEPDINHNYGLYLCQTAREPESIKYFLQAIRNPLYQAPWRSYAAAGTCSLRVK
                     NVKDADEFFQRALKMEPDEPSSLLQLGQIRYQQGRTDDARTLVSRHNKVVTPSAESLW
                     LALRIERKTGQRAAEQSYATQLRRRYPTSDEYRKLQRGEFD"
     gene            complement(2204..>2340)
                     /locus_tag="VGO02_07965"
     CDS             complement(2204..>2340)
                     /locus_tag="VGO02_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEV7477657.1"
                     /translation="AGVTVTTRKTRGDDIAAACGQLAGDVADRTRRSARRVALKEVRA
                     "
BASE COUNT          310 a          780 c          843 g          407 t
ORIGIN      
        1 cccagctctt cggcgtagcg cgccgaatcg agcgccgagc ggatgacggc ttcccgggtt
       61 actgcaacag agtctgaagg ttgagaaagc cgggcattct catccatcaa acgcgcgagg
      121 aggtcggcgt cgaggctgcc ccagttgacg ccgatgcgca ccggcttggc gtagcgcagg
      181 gcgcactcga tcatggtggc gaactgctcg tcgtgcttct tccccttgcc gacgttgccg
      241 gggttgatgc ggtacttggc gagcgcctgc gcgcagtccg ggaactgggt cagcagcttg
      301 tggccgttgt agtggaagtc gcccaccagc ggcacgcggc agccgatcgc gtccagccgt
      361 gcgcggatac gcggcacctg ggcggccgcc tccggcgtgt tgacggtgat gcgcacgagt
      421 tccgagccgg cgcgcgccag ctcttccacc tggcgcgccg tggactgcgc gtcctcggtg
      481 tcggtgttgg tcatcgactg caccaccacg ggagcgccgc cgccaatgag gacatcgccg
      541 accgcgacct ggcgcgaggt cctgcgcatg aaagtcatgg gatggtgaag cgcgccacct
      601 cgacccgggt gtacggcttg aggtcgagct cgtggtcgtt gtgcagcacg cgcacgtgcg
      661 acgcgttgcc gatgactaga gtgaacgggc cgcggccctg caccacgcgc tcgctgcccg
      721 ctcggttgag cgaagagacc agctggcggc cggcgccgtc ggtgacctcg agccaggcct
      781 cgtcggtctc gacgcgcagg acgatgcggt ggacaccgga ggcaggcggc gcggcgaccg
      841 tcttgactga aggagcgggc tttctttcga ccggctttgc ttcctccacc ttcttttcct
      901 cgatcttctt ttcctcgatc ttcttttctt ccgtcttctt ttcctcggcg atcggcgccg
      961 gaatgctcgc ctgctgggca ggtagcgcgg gcttgcgcgc cggcgcttcg gccttcggct
     1021 tgacgaaggc catctgcttg ggcgcgttgc gctcgtgctg ccactggtag gcgaccccgc
     1081 cgaccaccac caggatgacc agcgacaggc cgaaatagac cagcgtgctg cgccgcgcgt
     1141 tgtcggagaa cggcaccggc tggctgaagc gcgcggcgag ctggttggag tccggcgcgt
     1201 cgtagcggtc ggcgatgcgc gcgagcagcg gatcggggtc gattttcagg agccgcgcgt
     1261 agttgcgcac catgccgcgg gcgaaggtgc cgccgggcag ctggtcgaag cggtcggcct
     1321 ccagcgcctc gagctggcgc aggccgaact tgagctgctg cgcgacctcg ggcaggccga
     1381 gtcccagctc ctgccgggct gccgaaagcg cggcgccgac gctgccgtca gtcaaactcg
     1441 ccccgctgga gcttgcggta ctcgtccgac gtggggtagc gccgccgcag ctgcgtggcg
     1501 tagctctgct cggcggcgcg ctgtcccgtc ttgcgctcga tgcgcagcgc gagccacagc
     1561 gactcggcgc tcggcgtcac caccttgttg tggcgcgaaa ccagcgtgcg cgcgtcgtcc
     1621 gtgcggccct gctgatagcg gatctgcccg agctgcagca gcgagctcgg ctcgtcgggc
     1681 tccatcttca gggcgcgctg gaagaattcg tcggcgtcct tgacgttctt gacgcgcagg
     1741 ctgcaggtgc cggccgccgc gtaggaacgc cagggcgcct ggtacagcgg gttgcggatc
     1801 gcctgcagga agtacttgat cgactccggc tcgcgcgcgg tctggcacag gtacaggccg
     1861 taattgtggt tgatgtcggg ctcgttcggc gccaggcgca gcgcgcgatc gaagttctgc
     1921 tgcgccaggc tgttctcctt gaggtccatg tagaccaggc cgaacaggcc ataggccgtg
     1981 gcgtagttgg agtcggcagc caccgcggcg cgcagctcct ccagcgcgac gttcatgttg
     2041 ccgcggctgt agtagaggcc ggcgagctcg gtatgcagcc gcgcgcggtt gcgcgggtcg
     2101 cccgactcgc cggtcacctg gccggtctcg accgagggtc cctcgaccgg ctgggcggcg
     2161 cagcctgcga gcaacgccgc cgcggcgagg aacagtgcgc gcctcacgcg cgcacttcct
     2221 tcagcgccac ccggcgggcg ctgcggcggg tgcggtcggc gacgtcgccg gcgagctggc
     2281 cgcaggcggc ggcgatgtcg tcgccgcgcg tcttgcgcgt ggtcaccgtg accccggcgc
//
LOCUS       DASYJA010000361         3798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2685079, whole genome shotgun sequence.
ACCESSION   DASYJA010000361 DASYJA010000000
VERSION     DASYJA010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2685079"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /gene="rpoN"
                     /locus_tag="VGO02_07970"
     CDS             <1..825
                     /gene="rpoN"
                     /locus_tag="VGO02_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEV7477658.1"
                     /translation="VPCEGDDAPSHVLALEIAEKHLELLAGRDYTRLKSVTGANEETL
                     RAAQRLIQTLNPRPGAAFARVEARYVIPDVVVRKSKNVWRASLNPDAMPRLRINRLYA
                     EIAASARGGAGGASVSSQLQEARWLIKNVQQRFETILRVSQAIVDRQRHFLEHGEVAM
                     RPLVLREIAGTLGLHESTISRVTTQKFMATPRGTFELKYFFGSHVATEAGGAASSTAI
                     RALIKQLVGAENAKAPLSDSRIAQILGEQGIVVARRTIAKYREALQIPPVNQRKAL"
     gene            848..1156
                     /gene="raiA"
                     /locus_tag="VGO02_07975"
     CDS             848..1156
                     /gene="raiA"
                     /locus_tag="VGO02_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEV7477659.1"
                     /translation="MNLSVSGHHLEVTPALKSYVQAKLERVTRHFDHVIDAHVILTVE
                     KLKQKAEVTLHVRGKDLHCESEEGDLYAAIDLLADKLDRQVLRYKDKLYDKRGPDLAT
                     "
     gene            1153..1638
                     /gene="ptsN"
                     /locus_tag="VGO02_07980"
     CDS             1153..1638
                     /gene="ptsN"
                     /locus_tag="VGO02_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEV7477660.1"
                     /translation="MSLVAKLLSPTHVLTDLQVSSKKRLFEQAGLFFENHHRIARSLV
                     FDSLFAREKLGSTGLGQGVAIPHGRIKGLKEALGAFLRLAQPVPFDAPDGNPVSLVFV
                     LLVPEKATEKHLQILSELAQMFSDKALREAMNAAADAAALHHLITAWQPDAPGQRRAA
                     A"
     gene            1607..2518
                     /gene="hprK"
                     /locus_tag="VGO02_07985"
     CDS             1607..2518
                     /gene="hprK"
                     /locus_tag="VGO02_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEV7477661.1"
                     /translation="MHQVNVARLHDDNREALSLAWVAGRDGGTAVRSEAVAAAALIGH
                     LNLTHPNSIQVIGAYEAGLVSGFIERLFESQPAAIIVADGVKPPAALVEAATRAHTPL
                     FASPLPAPRVIEKLSRYLAKALAAHEERHGVFMDVLGLGVLITGDSGVGKSELGIELI
                     SRGHGLVADDVVDLSRIGDGGIEGRCPPMLKDFIEVRGLGLLNIRTIFGETAVRRKMK
                     LRLITHLAKPLPGGRDPTERLPLAELSEEILGVTVRKVIIPVAAGRNLAVLVEAAVRN
                     EILKLRGIDSTQEFLNRQQQKMQQEED"
     gene            2533..3381
                     /gene="rapZ"
                     /locus_tag="VGO02_07990"
     CDS             2533..3381
                     /gene="rapZ"
                     /locus_tag="VGO02_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEV7477662.1"
                     /translation="MQLILVSGLSGSGKSIALHVLEDGGFYCVDNLPAGSLDQVLGYL
                     AEAGHERVAVSVDARSAALGALPEHIGRLRSRGVDCRVLYLEATTAALLRRFSETRRR
                     HPLAGTGIALQEAIERERTLLADVAPLGHRIDTSELQPRVLQNWIRDLLGIGAGTITL
                     LFESFAYRDGIPLDADWVLDARMLPNPHYEPQLRPFTGRDAAVCEYLGAEAAVGRWLE
                     DVHTFLARWLHEIVRENRSYVTVAIGCTGGRHRSVYLVERLADSFRGEWKVLVRHRGL
                     ARAAPE"
     gene            3378..>3798
                     /locus_tag="VGO02_07995"
     CDS             3378..>3798
                     /locus_tag="VGO02_07995"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEV7477663.1"
                     /translation="MTDDLPLFPLNTVLFPGGRLPLRIFEQRYMDMTKACLKDGSPFG
                     VCLIREGAEVGEPAIPAHVGTLARITTWDMPQLGVLHVIAVGAQRFRIKDHRVEASGL
                     SRGEVDLLEADQDCAIPADCARCVTLLARVIKEQSNLF"
BASE COUNT          615 a         1378 c         1268 g          537 t
ORIGIN      
        1 gtgccgtgcg agggcgacga tgcgccgtct catgtgctcg cgctcgaaat cgccgagaag
       61 cacctcgagc tgctcgccgg ccgcgactac acgcgcctga agagcgtcac cggcgcgaac
      121 gaggaaacgc tgcgagcggc gcagcgcctg atccagacgc tcaacccgcg ccccggcgcc
      181 gccttcgcgc gcgtcgaggc gcgctacgtg atccccgacg tggtggtgcg caagagcaag
      241 aacgtgtggc gcgccagcct caaccccgac gccatgccgc ggctgcgcat caaccgcctg
      301 tacgccgaga tcgcggccag cgcccgcggc ggcgccggcg gagcgagcgt gtcctcgcag
      361 ctgcaggagg cgcgctggct gatcaagaac gtgcagcagc gcttcgagac catcctgcgc
      421 gtgtcgcagg ccatcgtcga ccggcagcgc cacttcctcg agcacggcga ggtcgcgatg
      481 cggcccctgg ttctgcgcga aattgccggc accctcggcc tgcacgagag caccatctcg
      541 cgcgtcacca cgcagaagtt catggccacg ccgcgcggca ccttcgagct gaagtacttc
      601 ttcggcagcc acgtcgccac cgaggccggc ggcgccgcct cctcgaccgc gatccgcgcg
      661 ctgatcaagc agctggtcgg cgccgagaac gccaaggcgc cgctgtccga ctcgcgcatc
      721 gcccagatcc tcggcgagca gggcatcgtg gtcgcccgcc gcaccattgc gaagtaccgg
      781 gaagcgctgc agattcctcc cgtcaaccag agaaaggccc tgtaaccatg aaccgtggag
      841 acagcaaatg aacctaagcg tcagcggcca tcatctcgaa gtgaccccgg ctctcaagag
      901 ctacgtgcag gccaagctgg agcgcgtgac gcggcatttc gaccacgtca tcgacgccca
      961 cgtcatcctg acggtggaga agctcaagca gaaggccgag gtgacgctgc acgtgcgcgg
     1021 caaggacctg cactgcgaga gcgaggaggg cgacctgtac gcggcgatcg atctgctcgc
     1081 cgacaagctc gaccggcagg tgctacgcta caaggacaag ctgtacgaca aaagagggcc
     1141 cgacctcgca acatgagcct cgtcgccaag ctgctgtccc cgacgcacgt cctgaccgac
     1201 ctgcaggtgt cgagcaagaa gcgcctgttc gagcaggccg gcctgttctt cgagaatcac
     1261 caccgcatcg ccaggagcct ggtcttcgac agcctgttcg cgcgcgagaa gctcggctcg
     1321 acgggcctgg gccagggcgt cgccatcccg cacggccgca tcaagggcct gaaggaggcg
     1381 ctcggcgcgt tcctgcgcct ggcgcagccg gtgccgttcg acgcgcccga cggcaacccg
     1441 gtcagcctgg tctttgtgct gctggtgccg gagaaggcca ccgaaaagca cctgcagatc
     1501 ctctccgagc tggcgcagat gttcagcgac aaggcgctgc gcgaggccat gaacgccgcg
     1561 gcggatgctg cggcgcttca tcacttgatc accgcatggc agccggatgc accaggtcaa
     1621 cgtcgcgcgg ctgcatgacg acaaccgcga ggcgctgtcg ctcgcctggg tggccgggcg
     1681 cgacggcggc accgcggtgc gcagcgaggc cgtcgcggcc gccgcgctga tcggccacct
     1741 caacctcacc caccccaaca gcatccaggt gatcggcgcg tacgaggccg gcctggtgtc
     1801 cggcttcatc gagcgcctgt tcgagtcgca gcccgccgcg atcatcgtcg ccgacggcgt
     1861 caagccgccc gccgcgctgg tcgaggccgc gacgcgcgcg cacacgccgc tcttcgcctc
     1921 gccgctgccg gcgccgcgcg tcatcgagaa gctctcgcgc tacctcgcca aggcgctcgc
     1981 cgcgcacgag gagcgccacg gcgtgttcat ggacgtcctc ggcctgggcg tgctgatcac
     2041 cggcgactcg ggcgtgggca agagcgagct cggcatcgag ctgatcagcc gcggccacgg
     2101 cctggtggcc gacgacgtgg tcgacctctc gcgcatcggc gacggcggca tcgaaggacg
     2161 ctgcccgccg atgctgaagg acttcatcga ggtgcgcggc ctcgggctgc tgaatatccg
     2221 caccatcttc ggcgagaccg cggtgcggcg caagatgaag ctgcgcctca tcacccacct
     2281 ggcgaagccg ctgcccggcg ggcgcgatcc gaccgagcgg ctgccgctcg ccgagctgtc
     2341 ggaggagatc ctgggcgtga cggtgcgcaa ggtgatcatc ccggtggccg ccgggcgcaa
     2401 cctcgccgtg ctggtcgagg cggcggtgcg caacgaaatc ctcaagctgc gcggcatcga
     2461 ttcgacgcag gaattcctca accggcagca gcagaagatg cagcaggaag aagactaagg
     2521 tgaagcgggg acgtgcagct catccttgtt agcggcctgt cgggctcggg caagagcatc
     2581 gcgctgcacg tgctcgagga cggcggcttc tattgcgtcg acaacctgcc ggcgggctcg
     2641 ctcgaccagg tgctgggcta ccttgccgag gccgggcacg agcgcgtcgc ggtgagcgtc
     2701 gacgcgcgca gcgcggcgct gggggcgctg cccgagcaca tcggccgcct gcgcagccgc
     2761 ggcgtcgact gccgcgtgct ctacctggag gccaccaccg cggcgctgct gcggcgcttc
     2821 tcggagacgc gccgccgcca tccgctcgcc ggcaccggca tcgccctgca ggaggcgatc
     2881 gagcgcgagc gcaccctgct cgccgacgtc gcgccgctcg gccaccgcat cgacacgagc
     2941 gagctgcagc cgcgcgtgct gcagaactgg atccgcgatc tgctcggcat cggcgcgggc
     3001 accatcaccc tgctgttcga atccttcgcc tatcgcgacg gcatcccgct cgacgccgac
     3061 tgggtgctcg atgcgcgcat gctgcccaac ccgcactacg agccgcagct gcgccccttc
     3121 accgggcgcg atgcggcggt gtgcgagtac ctgggcgcgg aagccgcggt cggacgctgg
     3181 ctggaagacg tgcacacctt cctcgcgcgc tggctgcacg agatcgtgcg cgagaaccgc
     3241 agctacgtga ccgtggcgat cggctgcacc ggcggccggc accgctcggt ctacctcgtc
     3301 gagcgcctcg ccgactcgtt ccgcggcgaa tggaaagtcc tcgtgcgcca ccgcggcctc
     3361 gcgcgcgcgg cgccggaatg acggacgacc tcccgctctt ccccctcaac accgttctct
     3421 tccccggcgg ccgcctgccg ctgcgcatct tcgagcagcg ctacatggac atgaccaagg
     3481 cctgcctgaa ggacggctcg cccttcggcg tctgcctgat ccgcgaagga gccgaagtgg
     3541 gcgagcccgc catcccggcg cacgtcggca cgctcgcccg catcaccact tgggacatgc
     3601 cgcagctcgg cgtgctgcac gtgatcgccg tcggcgcgca gcgttttcgc atcaaggacc
     3661 accgcgtcga ggcgagcggc ctgtcgcgcg gcgaggtcga cctcctcgaa gccgatcagg
     3721 actgcgccat cccggctgac tgcgccaggt gcgtcacgct cctcgcgcgc gtcatcaagg
     3781 agcagtccaa cctcttcg
//
LOCUS       DASYJA010000362         2492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2140145, whole genome shotgun sequence.
ACCESSION   DASYJA010000362 DASYJA010000000
VERSION     DASYJA010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2140145"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VGO02_08000"
     CDS             <1..203
                     /locus_tag="VGO02_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477664.1"
                     /translation="LGLLQTRTQSKATGAAAAESNHSELEMHVGIGGMLLLGRGWSLR
                     LEFERVNDSKIDLSTLGAQYQF"
     gene            226..1263
                     /gene="ruvB"
                     /locus_tag="VGO02_08005"
     CDS             226..1263
                     /gene="ruvB"
                     /locus_tag="VGO02_08005"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV7477665.1"
                     /translation="MSVETDRLISAKPSSPQEQQFERSLRPATLGEYVGQEKVRGQLE
                     IFIQAARARGEALDHVLLFGPPGLGKTTLAHIIARELGVNLKQTSGPVLERPGDLAAI
                     LTNLEAKDVLFIDEIHRLSPIVEEILYPALEDYQIDIMIGEGPAARSIKLDLPPFTLV
                     GATTRAGMLTNPLRDRFGIVSRLEFYSQSELAQIVQRSARLLQVSLEEEGASEISRRA
                     RGTPRVANRLLRRVRDYAQVKANGVVTKPVADAALKMLDVDSLGLDVMDRKLLLAVIE
                     KFSGGPVGLENLAHAIGEEADTIEDVLEPYLIQQGYLQRTLRGRMATLTAYRHFGIAA
                     PAVTRDLLSDN"
     gene            1274..1684
                     /gene="ybgC"
                     /locus_tag="VGO02_08010"
     CDS             1274..1684
                     /gene="ybgC"
                     /locus_tag="VGO02_08010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEV7477666.1"
                     /translation="MKSPFSIPVRVYYQDTDAGGVVFHAQYLAFMERARTELLNAARI
                     DLARVADEDRILFLVFEISAKYHQPARLNDMLAVSAEVVKMGRASLLVRHRVERAAQL
                     LVEADVTLALVDRDRMKPARLPAKLAEELAQALK"
     gene            1681..2367
                     /gene="tolQ"
                     /locus_tag="VGO02_08015"
     CDS             1681..2367
                     /gene="tolQ"
                     /locus_tag="VGO02_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEV7477667.1"
                     /translation="MNLTQDLEIWTLILNASIVVKVVMLVLLAVSFMSWMFIFQKWFS
                     IRRAGRQTELFEREFWGGRDLNALYQGAVNNRHSIGNLERIFEAGFREFAKLRGQKGA
                     NTDASDMVDGARRAMRATFQREMDFLERHLAFLASTGSVSPYVGLFGTVWGIMHSFRS
                     LANVQQATLAQVAPGIAEALVATAIGLFAAIPAVVAYNRYSYDIERLANRFESFMEEF
                     SNILHRQAVR"
     gene            2401..>2492
                     /locus_tag="VGO02_08020"
     CDS             2401..>2492
                     /locus_tag="VGO02_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEV7477668.1"
                     /translation="MHEINVVPYVDVMLVLLVIFMVTAPLVTPAV"
BASE COUNT          417 a          854 c          816 g          405 t
ORIGIN      
        1 ggctgggcct gctgcagacc aggacgcagt cgaaggcgac gggtgcggct gccgccgaga
       61 gcaaccacag cgagctcgag atgcacgtcg gcatcggcgg catgttgctt ctcgggcgcg
      121 gctggagcct gcgcctcgag ttcgagcgcg tgaacgacag caagatcgat ctctccacgc
      181 tcggcgcgca gtaccagttc tgacccgctc tggcagaatg cgcggatgag cgtcgagaca
      241 gaccgcctca tctcggccaa gccgtcgtcg ccacaggagc agcagttcga gcgctcgctg
      301 cgcccggcga cgctcggcga atacgtcggc caggagaagg tgcgcggcca gctagagatc
      361 ttcatccagg cggcgcgcgc gcgcggcgag gcgctcgatc atgtcctgct gttcgggccg
      421 cccgggctgg gcaagaccac cctcgcgcac atcatcgccc gcgagctggg cgtcaacctg
      481 aagcagacct cggggccggt gctcgagcgc ccgggcgatc tggccgccat cctgaccaac
      541 ctcgaggcca aggacgtcct gttcatcgac gagatccacc gcctgtcgcc gatcgtcgag
      601 gagatcctgt acccggcgct cgaggactac cagatcgaca tcatgatcgg cgaggggccg
      661 gcggcgcgct cgatcaagct cgacctgccg ccgttcacgc tggtgggcgc gacgacgcgc
      721 gccggcatgc tgaccaaccc gctgcgcgac cgcttcggca tcgtctcgcg gctcgagttc
      781 tattctcaaa gcgagcttgc gcaaatcgtg cagcgctccg cacgcttgct gcaggtgtcc
      841 ctcgaagagg agggcgcctc ggagatctcg cgtcgcgcgc gcggcacgcc gcgcgtcgcc
      901 aaccggctgc tgcggcgagt gcgcgactat gcccaggtca aggcgaacgg cgtcgtcaca
      961 aaaccggtgg ccgatgcggc gctcaagatg ctggatgtcg actccctcgg cctggatgtg
     1021 atggatcgca agctgctgct cgcggtgatc gagaagtttt ccggcgggcc ggtcggcctg
     1081 gagaacctgg cgcacgccat cggcgaggag gccgacacca tcgaggacgt gctcgagccc
     1141 tatctcatcc agcagggcta cctgcagcgc acgctgcgcg ggcgcatggc cacgctcacc
     1201 gcctaccgcc acttcggcat cgccgcgccg gcggtcacgc gcgacttatt gtcggataac
     1261 tgaagcggga caattgaaaa gtcccttcag tatccccgtg cgcgtgtact accaggacac
     1321 ggacgcgggc ggcgtcgtgt tccacgccca gtacctcgcc ttcatggagc gcgcgcgcac
     1381 cgaattgctc aacgccgcgc gcatcgacct ggcgcgcgtc gccgacgagg accggatcct
     1441 gttcctggtg ttcgagatct cggccaaata ccaccagccg gcgcggctga acgacatgct
     1501 cgccgtcagc gccgaagtgg ttaaaatggg ccgcgcgagc ctgctggtcc gccaccgcgt
     1561 ggaacgcgct gcacagctgc tggtcgaagc cgacgtgacg ctcgcgctgg tggaccgcga
     1621 ccgcatgaag ccggcgcggc tgccggccaa gctcgccgaa gaactcgcac aggcgctcaa
     1681 atgaacctga cccaagacct cgaaatctgg acgctcattc tcaacgcgag catcgtcgtc
     1741 aaggtggtca tgctggtgct gctcgcggtg tccttcatgt cgtggatgtt catcttccag
     1801 aagtggttct cgatccgccg cgccgggcgg cagaccgagc tgttcgagcg cgagttctgg
     1861 ggcggccgcg acctgaacgc gctctaccag ggcgcggtca acaaccgcca ttcgatcggc
     1921 aacctggagc gcatcttcga agcgggcttc cgcgagttcg ccaagctgcg cggccagaag
     1981 ggcgccaaca ccgacgccag cgacatggtc gacggcgcgc gccgcgcgat gcgcgccacc
     2041 ttccagcgcg agatggattt cctcgagcgg cacctggcgt tcctcgcctc gaccggctcg
     2101 gtcagcccct atgtcggcct gttcggcacg gtgtggggca tcatgcattc gttcaggagc
     2161 ctggccaacg tgcagcaggc gacgctcgcg caggtcgcgc cgggcatcgc cgaggcgctg
     2221 gtcgccaccg cgatcggcct gttcgccgcg atcccggcgg tggtcgccta caaccgctac
     2281 tcgtacgata tcgagcgcct cgccaaccgc ttcgagagct tcatggagga gttctcgaac
     2341 atcctgcacc gacaggccgt gaggtagagc gtggccacgc tgcgtcgcaa gcggggcccg
     2401 atgcacgaga tcaacgtcgt gccctacgtc gacgtgatgc tggtccttct cgtcatattc
     2461 atggtcaccg cgcctctggt caccccggcg gt
//
LOCUS       DASYJA010000363         2430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_597926, whole genome shotgun sequence.
ACCESSION   DASYJA010000363 DASYJA010000000
VERSION     DASYJA010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597926"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VGO02_08025"
     CDS             complement(<1..340)
                     /locus_tag="VGO02_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477669.1"
                     /translation="MSFGALLHSELPWLALIALALSGVLLHTRRHERKEYQNTLWLFL
                     FGVLGQIVAVAVHALDFPAAAAAVHMVFRVVAAIALIRLLGFVAFRLALPFAGRTPPR
                     IVEDLTIIAAY"
     gene            502..861
                     /locus_tag="VGO02_08030"
     CDS             502..861
                     /locus_tag="VGO02_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477670.1"
                     /translation="MPIISRHWSFNPLVISGAPEEPGVYALFEDDEIVYYGCAMQGST
                     IQSALSEILARVRAGRAGCLQRVNRYTWEITYRPRLREAELLREFEAANQHPPRGNQP
                     ELAAQAASSVVGQRRGT"
     gene            915..>2430
                     /locus_tag="VGO02_08035"
     CDS             915..>2430
                     /locus_tag="VGO02_08035"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEV7477671.1"
                     /translation="MPTILVADDHRANRDALGFLLEEAGYRVLSAANGAEALAMALEH
                     QPDLVISDVLMPKMDGYGLARRLRADPATAATRLMFYTAYFDRREAKELARAHGVARV
                     LAKPSENDDILRAVREALAASATPLEAVAPDLDRDRLRMMVDQLLEKTDALEAEQRRV
                     ERLNRTLSVLSAVNALIARLPERQALLEEACRIAVEKGGFGFAEITLHGAGKELGASR
                     GEPEVAFPAEVVLPLKVSEELAGSLLLRARAPDFFDEEEMRLLNELAADVSYALHHLE
                     QKARMDYLACHDSLTDLPNRSLFADRVAQALGAARRERRFAAALFLDIERFRMVNETF
                     GRKSGDDLLREVARRLRAAAREQDTVARVGADHFAIAVAGFDHPGDTTHWFMERLERE
                     FAEAIVIDGVELLVTLKAGVAVFPTDGESPEALCGNAETALRQAKQTGQRYLFYAPHM
                     NAQVAESLAMEHRLRRALEEGRLALHYQPKVDLRSGALTGLEALIRWHDAELGVV"
BASE COUNT          399 a          846 c          850 g          335 t
ORIGIN      
        1 cataggccgc gatgatcgtc aggtcctcga cgatgcgcgg cggcgtgcgc ccggcgaagg
       61 gcagcgccag gcggaaggcg acaaagccga gcaggcggat gagcgcgatc gcggcgacga
      121 cgcggaacac catgtgcacc gccgctgcgg ctgccggaaa gtcgagcgcg tggaccgcca
      181 ccgccacgat ctggccgagc acgccgaaga ggaacagcca cagcgtgttc tggtattcct
      241 tgcgctcgtg ccggcgggtg tggagcagca cgcccgacag agcgagcgcg atgagcgcca
      301 gccagggcag ctccgagtgc agcagcgcgc cgaagctcac agccggatcc agaaatccgc
      361 aaagcgtgaa gaagtgccgg cactcatccg cagattgtcg ttcagccgtg acagagctgt
      421 agccgtaatt ctacagttta catcacgaga tcattgcaag cgtttgagat gtcataaaaa
      481 agggcgtacc gtcgagggga aatgccgatc atcagccgcc attggagctt caaccccctg
      541 gtgatctcgg gcgcgccgga agagcccggc gtgtacgcgc tgttcgagga cgacgaaatc
      601 gtctactacg gctgcgcgat gcagggctcg acgatccagt ccgcgctctc cgagatcctc
      661 gcgcgcgtgc gcgccggccg cgccggatgc ctgcagcgcg tcaaccgcta cacctgggaa
      721 atcacctatc gcccgcggct gcgcgaggcc gagctgctgc gcgagttcga ggcagcgaac
      781 cagcatccgc cgcgcggcaa tcagcctgag ctcgccgcgc aagctgcctc gagcgtcgtc
      841 gggcagcgac gcggcacgta gcccgcgctg aggaacgatc gcgcgttcgc ccggcctaat
      901 cccgggttgc tctgatgcct accattctcg tcgccgacga tcaccgcgca aaccgcgacg
      961 ccctcggctt cctgctcgag gaggcgggct accgcgtgct gagcgcggcg aacggcgccg
     1021 aggcgctcgc catggcgctg gagcaccagc ccgacctcgt gatctccgac gtgctgatgc
     1081 cgaagatgga cggttatggc ttggcgcggc gcctgcgcgc tgatccggcg acggccgcga
     1141 cgcgcctgat gttctacacc gcgtatttcg accgccgcga ggcgaaagag ctggcgcggg
     1201 cgcacggcgt cgcgcgcgtg ctcgccaagc cctccgagaa cgacgacatc ctgcgcgcgg
     1261 tgcgcgaggc gctggcggcg agcgccacgc ccctcgaggc agtggcgccc gacctcgatc
     1321 gcgaccgcct gcgcatgatg gtcgaccagc tgctggagaa gaccgacgcg ctggaagccg
     1381 agcagcgccg cgtcgagcgc ctgaaccgca cgctgtcggt gctcagcgcc gtgaacgcgc
     1441 tgatcgcgcg cctgcccgag cgccaggcgc tgctcgagga ggcctgccgc atcgcggtgg
     1501 agaagggcgg cttcggcttc gccgagatca cgctgcatgg cgcaggcaag gagctgggcg
     1561 cgtcgcgcgg cgagccggag gtggcgtttc ccgccgaggt cgtgctgccg ctcaaggtgt
     1621 cggaggagct agccggcagc ctgctgcttc gcgcccgcgc gcctgacttc ttcgacgagg
     1681 aggagatgcg cctgctcaac gagctcgccg ccgacgtctc ctacgcgctg caccacctcg
     1741 agcagaaggc gcgcatggac tacctcgcct gccacgactc gctcaccgac ctgcccaacc
     1801 gcagcctgtt cgccgaccgc gtggcgcagg cgctgggcgc ggcgcggcgc gagcggcgct
     1861 tcgccgcggc cctgttcctc gacatcgagc gcttccgcat ggtgaacgag accttcggcc
     1921 gcaagtcggg cgacgacctg ctgcgcgagg tggcgcgccg cctgcgcgcc gcggcgcgcg
     1981 agcaggacac ggtcgcccgc gtcggggcgg accatttcgc catcgccgtc gccggcttcg
     2041 accatcccgg cgacaccacg cactggttca tggagcggct cgagcgcgag ttcgccgagg
     2101 cgatcgtgat cgacggcgtc gagctgctgg tgacgctgaa ggcgggggtg gcggtatttc
     2161 cgaccgacgg cgagtcgccc gaggcgctgt gcggcaacgc ggaaaccgcg ctcaggcagg
     2221 ccaagcaaac cggccagcgc tacctcttct acgcgccgca catgaacgcc caggtggccg
     2281 agtcgctcgc catggagcac cggctgcgcc gcgcgctgga agaaggacgc ctcgcgctgc
     2341 actaccagcc gaaggtcgac ctgcgcagcg gcgcgctcac cgggctggag gcgctcatcc
     2401 gctggcacga cgccgagctc ggcgtggtgc
//
LOCUS       DASYJA010000364         2306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2322079, whole genome shotgun sequence.
ACCESSION   DASYJA010000364 DASYJA010000000
VERSION     DASYJA010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2322079"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VGO02_08040"
     CDS             <1..890
                     /locus_tag="VGO02_08040"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEV7477672.1"
                     /translation="GTDALFLSLKALGIDKGDEVITTPYTFFATIGAIVTAGARPVFV
                     DIGDDYNIDPTKIEAAVTPRTRAILPVHWSGKPCDMDPIMEIAARRKLTVVEDACHAV
                     KATYRGRGAGVLGTVGCFSFHPLKNLNVWGDGGIATTNSEEIAAKLRLLRNHGLRNRD
                     ECEVFAYNSRLDTVQAVVARHLLQKLEHITESRIAHAAYFDNALSAIPQIRVPQREPW
                     IKQVYHLYIVRAERRDALQKFLAERDVDAKVHYPIPMHLQPAAREWGYKHGQFPIASR
                     STSTRMPACRGAGCSTPPG"
     gene            791..1345
                     /locus_tag="VGO02_08045"
     CDS             791..1345
                     /locus_tag="VGO02_08045"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477673.1"
                     /translation="MGLQARSVPDRFTLDLDPDAGLPWGRLLDATRLTKTLLDQLKLA
                     SFLKTTGGKGLHIVVPTEPKLGWHEVKEFTRRIALFLEKAQPDLFLANMSKAKRKGKI
                     FVDYLRNAETASAVAAYSPRARPEGGVSTPLAWDELGRADPRGRHTVKTVPLRLRRLR
                     ADPWKDYFSEKQAITAAMRRALGG"
     gene            1640..>2306
                     /locus_tag="VGO02_08050"
     CDS             1640..>2306
                     /locus_tag="VGO02_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEV7477674.1"
                     /translation="MPHLLLVDDDPEALEWLTELAKGEGFTVASADSLRNARIHMARL
                     QPDILLTDLQLPDGKGVELVADLETREATEVVMITGHASVESAVEALRLGATDYMVKP
                     VDVERLKAILRRVPRTGELRAEIGELRDELRKLGRFGHILGSSTPMQKLYDQLGRVAP
                     TSATVLLVGESGTGKELAAQTIHDLSRRKKSPFLPLNCGAVSPQLIESELFGHEKGSF
                     TGAD"
BASE COUNT          411 a          770 c          769 g          356 t
ORIGIN      
        1 gcggcacgga cgccctgttt ctcagcctga aggcgctcgg catcgacaaa ggcgacgagg
       61 tcatcactac gccgtatacg ttttttgcca cgatcggcgc aattgtgacg gcaggagcgc
      121 ggccggtgtt tgtcgacatc ggcgacgact acaacatcga cccgacaaag atcgaggctg
      181 cggtcactcc gcgcaccagg gccatccttc ccgtgcactg gtccggcaag ccctgcgaca
      241 tggacccgat catggaaatt gcggcgcgcc gtaagctgac cgtggtggag gacgcctgcc
      301 atgcagtcaa ggcgacgtac cgcggccgcg gagccggggt cctgggcacg gtgggctgct
      361 tcagcttcca tccgctgaag aacctcaacg tttggggaga cggcggcatc gccacgacca
      421 actcggagga aattgccgcg aagctgcgcc tgctgcgcaa ccacggcctg cgcaaccggg
      481 acgagtgcga ggtgtttgcc tacaactcgc gtctcgacac cgtgcaggcg gtcgtggcgc
      541 gccacctgct gcagaaactc gagcacatca ccgagtcgcg catcgcacac gccgcctatt
      601 tcgacaatgc gctaagcgcc atcccgcaga tccgggtgcc gcagcgcgag ccgtggatca
      661 agcaggtcta tcacctgtac atcgtacgcg ccgagcggcg cgacgccctg cagaagttcc
      721 tggccgagcg cgacgtggac gcgaaggtcc actatcccat tccgatgcac ctgcagccgg
      781 cggccaggga gtggggctac aagcacggtc agttcccgat cgcttcacgc tcgacctcga
      841 cccggatgcc ggcctgccgt ggggcaggct gctcgacgcc acccggctga cgaagacgct
      901 cctcgaccag ctgaagctcg cctcgttcct caagaccacc ggcggcaagg gactgcacat
      961 cgtggtgccg accgagccga agctgggctg gcacgaggtc aaggagttca cgcgccgcat
     1021 cgcgctgttc ctggagaaag cgcagccgga tctgttcctg gccaacatgt cgaaggcgaa
     1081 gcgcaagggg aagatattcg tcgactacct gcgcaacgcc gagacggcga gtgcggttgc
     1141 cgcttactcg ccgcgcgcgc ggcccgaggg cggcgtgtcg acgccgctcg cctgggacga
     1201 gctcggccgc gccgacccgc gcggccgcca cacggtgaag accgtgccgc tacggcttag
     1261 gcgcctgcgt gccgatccct ggaaggacta tttttccgaa aagcaggcga ttaccgcggc
     1321 gatgcggcgc gctctcggcg gatgacgctt ttcaaagctt ccgcccgggc gctgccgcgg
     1381 tggggagatt gcggtggagc gcagcggcga ccgggcggat gcgcagcgcg ttgcaggtaa
     1441 tgtgcccgcg gcgaattgcg tcgcctcgca gcgaattgcg tcgccgcgcg gcgacgaaat
     1501 cgcccgccgc atgcggaaaa attgcgtgta gccgttttca catgctcagg taaatcaatg
     1561 aggtgcgtca ccgggggcgg gtggtacgca agttgcgttc ctttgcgaac tatttacccg
     1621 cccggagtaa atcctacaga tgccgcatct cctgttggtc gacgacgatc ctgaagcgct
     1681 ggagtggctc accgagctgg cgaaaggcga aggcttcacc gtcgcctcgg ccgattcgct
     1741 gcgcaacgcg cgcatccaca tggcgcgcct gcaacccgac atcctgctga ccgacctgca
     1801 gctgcccgac ggcaagggcg tcgagctggt cgccgacctc gagacgcgcg aggcgaccga
     1861 ggtggtgatg atcaccggcc acgccagcgt ggagagcgcg gtcgaggcgc tgcgcctggg
     1921 cgccaccgac tacatggtga agccggtcga cgtcgagcgc ctgaaggcga tcctgcgccg
     1981 cgtgccgcgc accggcgagc tgcgcgccga gatcggcgag ctgcgcgacg agctgcgcaa
     2041 gctcggccgc ttcggccaca tcctgggctc ctcgacgccg atgcagaagc tctacgacca
     2101 gctcgggcgc gtcgcgccga cttcggccac cgtgctgctg gtgggcgaga gcggcaccgg
     2161 caaggagctg gcggcgcaga ccatccatga cctgagcagg cggaagaagt cccccttcct
     2221 gcccctgaac tgcggcgcgg tatcgccgca gctcatcgaa agcgagctgt tcggccacga
     2281 gaagggctcg ttcaccggcg ccgacc
//
LOCUS       DASYJA010000365         5095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2232207, whole genome shotgun sequence.
ACCESSION   DASYJA010000365 DASYJA010000000
VERSION     DASYJA010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2232207"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VGO02_08055"
     CDS             <1..749
                     /locus_tag="VGO02_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEV7477675.1"
                     /translation="VKVICKQGVGFDTIDIQAAAKHGIPVLRTPGVNSEAVAEMAMAL
                     ALSATRRVTETDRMLRAGAKIERPDILGIEMWQKTVGVIGLGNIGSRVARKWQGAFEA
                     NVIFFDPYVQSKPPYRKVSHLDELLKEADLVSIHVPLTDETRKMIGAPQLALMKHTAV
                     LVNTSRGGIIDEAALYQALKSGRLFAAGLDVWEVELPPRDAPLLSLPNVVGTPHAAGG
                     TFDTQERSSMQVAIQVVEVLQGKPPQNRVN"
     gene            751..1569
                     /locus_tag="VGO02_08060"
     CDS             751..1569
                     /locus_tag="VGO02_08060"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEV7477676.1"
                     /translation="MEIRALTANTGVEVLGVDLRGVVEKKKLNDLLAKHSVVVFREQK
                     LGPPEYMKAAQIWGEIMPQMLERFTLPEYPLVGFVSSEDTDKPGGKRMIRGEQYHTDH
                     SNYPAPPKATLLHAVTIPSVGGDTQFVNVHASYEDLSQQTKSKIDGLKVLHVYLSSRS
                     PRRKAVLSEEEKNRIPETWQPLVPRHPESGRKGLYLNTAHMERIEGMADEEAFKLIGE
                     LMAHATQPKYEYRHKWRDGDMVIWDNRSVMHQANGDYAEKRYLLRLMVKGVPLS"
     gene            complement(1566..2438)
                     /locus_tag="VGO02_08065"
     CDS             complement(1566..2438)
                     /locus_tag="VGO02_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7477677.1"
                     /translation="MKQFVLAVLALACLPVWAQAPARPMIETTKVEGTDNVYIFRHGN
                     HQSIFVVTPEGVIATDPVAYGRPQGGAAYVAEIRKVTDKPIKYLVYSHHHYDHIAGGQ
                     AFKDAGAKIVAHSKAKERLAVLKDPNTPLPDETFAKSRTLKLGGTALEMTYVGLNHSD
                     STLVFRLPKEKLLFVVDLIPVGQFPGRGMIDFYPLEAQDSIKRILAMDWERLIPGHPG
                     VGGRLGTKKDAQDQLELLQYASDTVKGPAQEGKCWDGVEKETKLPKYENWPGYENGLP
                     LVLRRYCGLWGRGT"
     gene            complement(2449..3528)
                     /locus_tag="VGO02_08070"
     CDS             complement(2449..3528)
                     /locus_tag="VGO02_08070"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEV7477678.1"
                     /translation="MKITAVDTCVLTIPTRHQMALQFPHHKFVVAEISTDSGAKGLGY
                     SMVFNGGGAEAVHAYLETRLKPALLGEDPLYVERLWEKMFRVDMGIKKQGVAAYALSA
                     ADIALWDLVAKVAKLPLHKLWGAHSDLVPCYGSGGWGKYSEKDLIAEAERYASRGVKY
                     YKMKLHDPDLKANSKRVEAVHRALGGKMRMMVDINQRLDVLGNVRQAALLEEFDLLWY
                     EEPVLADDLSALQQVAERIRIPIATGENNFTRWEFRDLIERKAATFLMPDVCRANGYS
                     ETMRIGRLAAAHQLQVAPHVAHEISLPVVGALPNGFLVEYMDWHPDDLFEETPACREG
                     AFRIGDKPGHGISLTREAFRKYRTT"
     gene            3619..5085
                     /locus_tag="VGO02_08075"
     CDS             3619..5085
                     /locus_tag="VGO02_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477679.1"
                     /translation="MSFQSWLLWPENVGLSLLVLVVLAMAFLYAARRPMHELIRSLGQ
                     ALGGPLRLGARWLFATADEMTRRNKAVLLAHGRQEVGARIEREFERLGALVERDLQGY
                     PTLQRKLLDEITRIEEDYKKCGEVPPPPPDWVEAVAAIANVKSGGGELVLKVLEEIKR
                     SITTIHDKAIGEYRHSYEERHKILEGFMPFWRSVDKNLAQVDKNLAALQASATTVDSH
                     MERYEQINKGTDKAEQALTVSAFTQFAIAALVMTVAAGGAFINYKLIALPMSEMVGAG
                     DYITASLRTSEVAALVIIFVEASMGLFLLEAMRITHLFPRIANLNDQMRRRMMWIALA
                     LLVTLAGVEAALALMRDMLIADKQALLQSLATVQAAPTEGWVGRIPTAGQMLLGFILP
                     FALAFIAIPLESLIHSSRTVGGVFLAAAVRALAFALRLAGNIAKHACKVLVRLYDVAI
                     VLPLLAERMVRGARHRHAGKRGPKAANDVDIDVERTHA"
BASE COUNT          874 a         1668 c         1681 g          872 t
ORIGIN      
        1 aggtgaaggt gatctgcaag cagggcgtcg gcttcgacac catcgacatc caggcggcgg
       61 cgaagcacgg catcccggtg ctgcgcacgc ccggcgtcaa cagcgaggcg gtggcggaga
      121 tggcgatggc gctcgcgctc tcggcgacgc gccgcgtcac cgagaccgac aggatgctgc
      181 gcgccggggc gaagatcgag cggcccgaca tcctcggcat cgagatgtgg cagaagaccg
      241 tcggcgtgat cggcctgggc aacatcggct cgcgcgtggc gcgcaagtgg cagggggcat
      301 tcgaggcaaa cgtgatcttt ttcgatccgt acgtccaatc caagcctccc tatcggaagg
      361 tttcccattt ggatgaattg ctgaaagagg cagatcttgt aagcatccac gtgccgctga
      421 ccgacgagac tcgaaagatg atcggcgcgc cgcagctcgc gctgatgaaa cacaccgccg
      481 tcctggtcaa cacctcgcgc ggcggcatca tcgatgaagc ggcgctgtat caagcgctga
      541 agagcgggcg cctcttcgcc gccggcctgg acgtctggga agtcgagctg ccgcccaggg
      601 acgcgccgtt gctttcgctg cccaacgtgg tcggcacgcc gcacgccgcc ggcggcacct
      661 tcgatacgca ggagcgcagc tccatgcagg tcgcgatcca ggtggtcgag gtgctgcagg
      721 gcaagccgcc gcagaacagg gtgaactgac atggaaatcc gcgcattgac ggcgaatacc
      781 ggggtcgaag ttctcggcgt cgatcttcga ggcgtcgtgg agaaaaagaa gctcaacgac
      841 cttctggcaa agcactcggt ggtcgttttc cgcgagcaga agctcgggcc gccggagtac
      901 atgaaggcgg cgcagatctg gggcgagatc atgccgcaga tgctcgagcg cttcacgctg
      961 cccgagtatc cgctggtcgg cttcgtctcg agcgaggaca ccgacaagcc cggcggcaag
     1021 cgcatgatcc gcggcgagca gtaccacacc gaccactcca actacccggc gccgccgaag
     1081 gccacgctgc tgcacgcggt gacgataccg agcgtgggcg gcgatactca gttcgttaac
     1141 gtgcacgcaa gttatgaaga cctttcacaa caaaccaaat caaagatcga cggattgaag
     1201 gtcctgcatg tctacctgag cagccgcagc ccgcgcagaa aggccgtgct gagcgaagag
     1261 gagaagaacc gcatccccga gacctggcag ccgctcgtgc cgcgccaccc ggagagcggc
     1321 cgcaagggcc tctacctcaa caccgcccac atggagcgca tcgagggcat ggccgatgaa
     1381 gaggcgttca agctgatcgg cgagctgatg gcgcacgcca cgcagccaaa atacgagtac
     1441 cgccacaagt ggcgcgacgg cgacatggtg atctgggaca accgcagcgt gatgcaccag
     1501 gcgaacggag actacgccga gaagcgctat ctccttcggc tgatggtcaa aggtgtgcct
     1561 ctgagctagg ttccgcggcc ccacaagccg cagtagcggc gcagcacgag cggcaggccg
     1621 ttctcgtagc ccggccagtt ctcgtatttc ggcagcttgg tctccttctc gacgccgtcc
     1681 cagcacttgc cttcctgcgc cgggcccttc acggtatcgg aggcgtactg cagcagctcg
     1741 agctggtcct gcgcgtcctt cttcgttccc aggcgcccgc cgacgcccgg atggccgggg
     1801 atcaggcgct cccagtccat cgccaggatg cgcttgatgg agtcctgcgc ctcgagcgga
     1861 tagaagtcga tcatgccgcg ccccgggaac tggccgaccg ggatgagatc gaccacgaac
     1921 agcagcttct ccttcggcag gcggaagacg agcgtgctgt cggagtggtt gaggccgacg
     1981 taggtcatct cgagcgcggt gccgccgagc ttgagggtgc ggctcttggc gaaggtctcg
     2041 tcgggcagcg gggtgttcgg gtccttcagc accgcgagcc gctccttcgc cttgctgtgc
     2101 gccacgatct tcgcgcccgc gtccttgaac gcctggccgc cggcgatgtg gtcgtagtgg
     2161 tggtggctgt agaccaggta cttgatcggc ttgtcggtca ccttcctgat ctccgcgacg
     2221 taggcggcgc cgccctgcgg ccggccgtag gccaccgggt cggtggcgat cacgccttcg
     2281 ggcgtgacga cgaagatcga ctggtggttg ccgtggcgga agatgtagac gttgtcggtg
     2341 ccctcgacct tggtggtctc gatcatcggg cgggcgggtg cctgggccca gacgggcagg
     2401 catgcaagag caaggacagc caggacgaac tgtttcatct tcccctcctc aggttgtacg
     2461 gtacttcctg aacgcttccc gcgtcagcga tattccatgt ccgggcttgt cgccgatgcg
     2521 gaaggcacct tcgcggcagg caggtgtctc ctcgaaaagg tcgtccgggt gccagtccat
     2581 gtactccacc aggaagccgt tgggcagcgc cccgaccacc ggcagcgaga tctcgtgcgc
     2641 cacgtgcggc gcgacctgga gctggtgcgc ggccgccagg cggccgatgc gcatggtttc
     2701 gctatagccg ttggcgcggc acacgtcggg catcaggaag gtcgcggctt ttctttcgat
     2761 caggtccctg aattcccacc gggtgaaatt attttcaccc gttgcgatcg ggatcctgat
     2821 ccgctccgca acctgttgaa gcgcagaaag atcgtcggcg agcaccggct cttcatacca
     2881 gagcagatcg aactcctcga gtagcgcggc ctgtcgcacg ttgcccagca cgtcgaggcg
     2941 ctggttgata tccaccatca tgcgcatctt gccgccgagc gcccggtgca ccgcctcgac
     3001 ccgcttgctg ttcgccttca ggtcgggatc gtggagtttc atcttgtagt acttgacgcc
     3061 gcgcgaggcg tagcgctcgg cctcggcgat caggtccttc tccgagtact tgccccagcc
     3121 gccgctgccg tagcagggga cgaggtcgct atgcgcgccc cagagcttgt gcagcggcag
     3181 cttggccacc ttggcgacga ggtcccacag ggcgatgtcg gcggcggaaa gcgcgtacgc
     3241 cgcgacgccc tgcttcttga tacccatgtc gacgcggaac atcttctccc ataggcgctc
     3301 gacgtagagc gggtcctcgc cgaggagcgc cggcttgagc cgcgtctcga ggtaggcatg
     3361 caccgcctcg gcgccgccgc cgttgaacac catgctgtag ccaaggcctt tcgcgcccga
     3421 atcggtcgag atctccgcca ccacgaactt gtggtgcggg aactgcagcg ccatctggtg
     3481 gcgcgtcggg atggtgagga cgcaggtgtc gaccgcggtg attttcatca cgcgatgata
     3541 gtgccaaagt tcctcggcgc agggggcgtt tctggcctaa cctcttggtc ggggcccccg
     3601 ccccgtggga ggtattccgt gagctttcag tcctggctgc tgtggcccga aaacgtcggg
     3661 ctttccctgc tggttctcgt cgtgctggcg atggcattcc tttacgccgc gcgccggccc
     3721 atgcacgagc tgatccgctc gctcggccag gcgctcggcg gcccgctgcg cctcggcgcg
     3781 cgctggctgt tcgccaccgc cgacgagatg acccggcgca acaaggcggt gctgctcgcc
     3841 cacgggcgcc aggaagtcgg cgcgcgcatc gagcgcgagt tcgagcgcct cggcgcgctg
     3901 gtcgagcgcg acctgcaggg ctacccgacg ctgcagagga agctgctcga cgagatcacg
     3961 cgcatcgagg aggactacaa gaagtgcggc gaagtgccgc cgccgccccc ggactgggta
     4021 gaagcggtcg ccgccatcgc caacgtcaag tccggcggcg gcgagctggt gctcaaggtg
     4081 ctcgaggaga tcaagcgctc gatcaccacc atccacgaca aggcgatcgg cgagtaccgc
     4141 cattcctacg aggagcgcca caagatcctc gagggcttca tgccgttctg gcgctcggtc
     4201 gacaagaacc tcgcgcaggt cgacaagaac ctcgccgccc tgcaggcgag cgccaccacg
     4261 gtcgactcgc acatggagcg ctacgagcag atcaacaaag gcaccgacaa ggcggagcag
     4321 gcgctgaccg tgtcggcctt cacgcagttc gccatcgccg ccctggtgat gacggtcgcg
     4381 gccggcggcg cgttcatcaa ctacaagctg atcgcgctgc cgatgtccga gatggtcggc
     4441 gccggcgact acatcaccgc gtcgctgcgc acctccgagg tggccgcgct ggtgatcatc
     4501 ttcgtcgagg cgtcgatggg cctgtttctg ctcgaggcga tgcgcatcac gcacctgttc
     4561 ccgcgcattg cgaacctgaa cgaccagatg cgccggcgca tgatgtggat cgcgctcgcg
     4621 ctgctggtga ccctggccgg cgtcgaggcg gccctcgccc tgatgcgcga catgctgatc
     4681 gccgacaagc aggcgctgct gcaatcgctc gccacggtgc aggccgcgcc gaccgaaggc
     4741 tgggtcgggc gcatcccgac cgccggccag atgctgctcg gcttcatcct gccgttcgcg
     4801 ctcgccttca tcgccattcc gctggagtct ctgatccact cctcgcgcac ggtcggcggc
     4861 gtgttcctcg ccgcggcagt gcgcgcgctg gctttcgcgc tgcgcctcgc cggcaacatc
     4921 gcaaagcacg cgtgcaaagt gctcgtccgc ctgtacgacg tggcgatcgt gctgccgctg
     4981 ctcgccgagc ggatggtgag gggggcgcgc caccgccatg cgggcaagcg cgggccgaaa
     5041 gcagccaacg acgtcgatat cgacgtggag cgcacccatg cttaagaaac tgctc
//
LOCUS       DASYJA010000366         1828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2503426, whole genome shotgun sequence.
ACCESSION   DASYJA010000366 DASYJA010000000
VERSION     DASYJA010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2503426"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VGO02_08080"
     CDS             <1..431
                     /locus_tag="VGO02_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477680.1"
                     /translation="LKRFMIASTPKEFERERVSAAAFGRLTAEQSRELQQSRDLGNVP
                     AGDDYPAFLDRQPPGKAAALAELARQPDTLSILHGTRIPPARRDDKPQYLLSSTTLLL
                     VRGRVLSLAVYAAYESPADPDWIRGATLRWIEELKRLNGR"
     gene            435..1247
                     /locus_tag="VGO02_08085"
     CDS             435..1247
                     /locus_tag="VGO02_08085"
                     /inference="COORDINATES: protein motif:HMM:NF013788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HEV7477681.1"
                     /translation="MARLFLLLALACAASSAPCNPAEGDPRPPYAVRSALERLPPQRP
                     GTVDLYAIVVGGDAEEVFRREVETVRRVLDERLGTAGRSLAMVNDLTQPEPEVTLNSL
                     AYALRAVAKRMDPDEDVLLLHLTSHGLRNSELSLRHPATPLYSLSPLYLRSMLDEAGV
                     RNRVIIVSSCFSGGFVAPLATGDTLVLTAASADRPSYGCGSDSQITDFSRALYLKALS
                     RTRSLREAAAQTLELVRDDEKARGYTPSLPQLRSGAAIDERLRLIERQLGAR"
     gene            complement(1244..>1828)
                     /locus_tag="VGO02_08090"
     CDS             complement(1244..>1828)
                     /locus_tag="VGO02_08090"
                     /inference="COORDINATES: protein motif:HMM:NF015081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7477682.1"
                     /translation="DIESYADRVAAVLEALRLPRDTDILGNGFGGFIAVMLALRHGKR
                     FDRLIAAPALAGFPEAAKAPFHRMAGLVSSQGMGAVLDAAIQRMLPAAFIEKNPEIVA
                     QRRQSLAKADPACFATACLALARLDLSGVLQEIKNPTLVIAGSEDATTPPGLARELAA
                     GIPGAKFLELKGCGHCPQVENPGAFVAALEDFLG"
BASE COUNT          261 a          701 c          605 g          261 t
ORIGIN      
        1 atctgaagcg cttcatgatc gcctcgacgc cgaaggagtt cgagcgcgag cgggtcagcg
       61 ccgccgcctt cggccgcctg acggcggaac agtcgcgcga gctccagcag tcgcgcgacc
      121 tcggcaatgt ccctgccggc gacgactatc ccgccttcct cgacaggcag ccgcccggga
      181 aggccgctgc gctcgccgag ctggcgcgcc agcccgacac gctgtccatc ctgcacggaa
      241 cgcgcatccc cccggcgcgc cgcgatgaca agccgcaata cctcctctcg agcacgacgc
      301 tgctgctggt gcgcggcagg gtgctcagcc tggcggtcta cgccgcctac gaaagccccg
      361 ccgacccgga ctggatccgc ggcgccacgc tgcgctggat cgaggagctg aagcggctga
      421 acggccgcta gcgcttggcc aggcttttcc tcctcctcgc gctcgcctgc gccgcctcga
      481 gcgcgccgtg caacccggcc gaaggcgacc cgcgcccgcc ctacgccgtg cgctcggcgc
      541 tcgagcgcct gccgccgcag cggcccggca cggtcgacct ctacgccatc gtcgtcggcg
      601 gcgacgccga ggaagtgttc cgccgcgagg tggagacggt gcgccgcgtg ctggacgagc
      661 ggctcggcac cgccggccgc tcgctcgcca tggtcaacga cctgacgcag cccgagcccg
      721 aggtgacgct caactcgctg gcctatgcgc tgcgcgcggt cgccaaacgc atggacccgg
      781 acgaggatgt cctgctcctg cacctgacct cgcacggcct gcgcaacagc gagctgtcgc
      841 tgcgccaccc ggcgacgccg ctctactccc tgagcccgct gtacctgcgc tcgatgctgg
      901 acgaggcggg cgtgcgcaac cgcgtgatca tcgtttcctc ctgcttctcg ggcggcttcg
      961 tcgcgccgct cgccaccggc gacacgctgg tgctcaccgc cgccagcgcc gaccgcccct
     1021 cctacggctg cggcagcgac tcgcagatca cggatttctc gcgcgcgctg tacctgaagg
     1081 ccttgagccg cacccgctcg ctgcgcgagg cggcggcgca gaccctcgag ctggtccgcg
     1141 acgacgagaa ggcgcgcggc tacacccctt cgctgccgca gctgcgctcc ggcgccgcga
     1201 tcgacgagcg cctgcggctc atcgagcgcc agctcggcgc ccgctagccc aggaagtcct
     1261 ccagcgccgc gacgaaagcg ccgggattct cgacttgcgg gcagtgcccg cagcctttca
     1321 actccaggaa cttcgcgccc gggatgccgg ccgccagctc gcgcgccagg cccggcggcg
     1381 tggttgcgtc ttcgctgccg gcgatgacca gcgtcggatt ttttatctcc tgcagaaccc
     1441 ctgaaagatc cagcctggcg agcgcgaggc acgccgttgc gaagcaggcc ggatcggcct
     1501 tggcgaggga ttgcctgcgc tgcgcgacga tttcagggtt cttctcgatg aacgcggccg
     1561 gcagcatgcg ctggatcgcc gcgtcgagca cggcgcccat gccctgcgac gaaaccaggc
     1621 ccgccatgcg atggaacggc gccttcgccg cctcggggaa cccggcaagc gctggcgcgg
     1681 cgatcagccg gtcgaagcgc ttgccatgac gcaacgccag catcacggcg atgaaaccgc
     1741 cgaagccgtt gccgaggatg tcggtgtcgc gcggcagccg cagcgcttcg aggacggccg
     1801 ccacccggtc ggcgtagctc tcgatgtc
//
LOCUS       DASYJA010000367         1678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_508093, whole genome shotgun sequence.
ACCESSION   DASYJA010000367 DASYJA010000000
VERSION     DASYJA010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508093"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /gene="alr"
                     /locus_tag="VGO02_08095"
     CDS             complement(<1..658)
                     /gene="alr"
                     /locus_tag="VGO02_08095"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV7477683.1"
                     /translation="MPRPIRATVSAGALAHNLLVAKAYAGGGKLWAVLKANAYGHGLE
                     RAARALEGADGFAVLDFQEAARLRVAGIAKPILMLEGFFKPVDLPLLAKYALTPVIHN
                     AEQVELLTAAELGGEIDVYLKANSGMNRLGFTVEGLRAAYNSVRMHRQVRSVTLMSHF
                     ADADGPSGVQAQLQWLEEMARPIEGLARAPRSLANSAALIRFAEARAGWARPGIMLYG
                     C"
     gene            747..>1678
                     /gene="lplT"
                     /locus_tag="VGO02_08100"
     CDS             747..>1678
                     /gene="lplT"
                     /locus_tag="VGO02_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HEV7477684.1"
                     /translation="MNAGFYIIMAAQFFSSLADNALLVAAIALLMQADAPSWLTPYLK
                     FFFVISYVVLAPYVGVFADRLPKGQIMLLANAIKIVGCLVMLFGTDPLISYALVGLGA
                     AAYSPAKYGILTEYLPHRKLVIANGWIEGLTVASILLGTVLGGVLIHARVSGVLLAID
                     MPFIETSIDTPAEAAIALIVAVYAIAAVFNLHVPNTGVALKPLPANPVDTLQDFWRCV
                     RYLWRDRLGQISLATTTLFWGAGATLQFIVIEWARVALGFDLSRAAMLQGITAIGIAI
                     GAVLASLWVSLDRSVRVIPLGIVMGLVVIVMNYV"
BASE COUNT          240 a          589 c          559 g          290 t
ORIGIN      
        1 agcagccgta gagcatgatg ccggggcgcg cccagccggc gcgcgcttcg gcaaagcgga
       61 tcagcgccgc cgagttggcg agcgagcgcg gcgcccgcgc gagcccctcg atcggccgcg
      121 ccatctcctc gagccactgc agctgcgcct ggacaccgct cggcccgtcg gcatcggcga
      181 agtggctcat cagcgtcacg ctgcgcacct gccggtgcat gcgcacgctg ttgtaggcgg
      241 cgcgcaggcc ctcgacggtg aagcccagcc ggttcatccc gctgttcgcc ttcaggtaga
      301 cgtcgatctc gccgccgagc tcggcggccg tgagcagctc gacctgctcg gcattgtgga
      361 tgaccggcgt cagcgcgtac ttggcgagca gcggcaggtc gaccggcttg aagaagcctt
      421 ccagcatgag gatcggcttg gcgatgccgg cgacgcgcag ccgcgcagcc tcctggaagt
      481 cgagcaccgc gaagccgtcg gcgccttcga gggcgcgcgc ggcgcgctcg aggccatggc
      541 cgtaggcgtt cgccttgagc accgcccaga gcttgccgcc gccggcgtag gccttggcga
      601 ccagcagatt gtgcgcgagc gcgcctgccg agacggtggc gcggatgggg cgaggcatcg
      661 ggcgttataa accttcaggg ccgtaataac agaagcctat tgtgctataa cccgccgacc
      721 gtaaaaacta cgatgtgctg aaaggcatga acgccgggtt ctacatcatc atggcggcgc
      781 agttcttctc ctcgctcgcc gacaacgcgc tgctggtggc ggcgatcgcg ctgctgatgc
      841 aggccgatgc gccgtcctgg ctgacgccgt acctcaagtt cttcttcgtc atctcgtacg
      901 tggtgctcgc gccctacgtc ggcgtgttcg ccgaccgcct gcccaagggg cagatcatgc
      961 tgctcgccaa cgccatcaag atcgtcggct gcctggtgat gctgttcggc accgacccgc
     1021 tcatctccta cgccctggtc ggcctgggcg ccgcggccta ctcgccggcc aagtacggca
     1081 tcctcaccga gtacctgccg caccgcaagc tggtgatcgc caacggctgg atcgaagggc
     1141 tgacggtggc ctcgatcctg ctcggcacgg tgctcggcgg ggtgctgatc catgcgcgcg
     1201 tctccggcgt gctgctggcc atcgacatgc cgttcatcga gacctcgatc gacacgccgg
     1261 ccgaggcggc gatcgccctc atcgtggcgg tctacgccat cgccgcggtg ttcaacctgc
     1321 acgtgccgaa caccggcgtc gcgctgaagc cgctgccggc gaacccggtc gacacgctgc
     1381 aggacttctg gcgctgcgtg cgctacctct ggcgcgaccg gctggggcag atctcgctcg
     1441 ccacgacgac gctattctgg ggcgccggcg ccaccctgca gttcatcgtc atcgagtggg
     1501 cgcgcgtcgc gctcggcttc gacctgtcgc gcgccgccat gctgcagggc atcaccgcca
     1561 tcggcatcgc catcggcgcg gtgctcgcct cgctctgggt gtcgctcgac cgctcggtgc
     1621 gcgtgatccc gctcggcatc gtcatgggcc tggtggtgat cgtcatgaac tacgtgca
//
LOCUS       DASYJA010000368         4467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_872274, whole genome shotgun sequence.
ACCESSION   DASYJA010000368 DASYJA010000000
VERSION     DASYJA010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872274"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VGO02_08105"
     CDS             <1..293
                     /locus_tag="VGO02_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEV7477685.1"
                     /translation="RELVYQDLIRPMKQLRYVPIVSREAAPGALSGRIPAAIADGRLE
                     AAAGVRLAAETSQVMLCGNPQMLKDAAAVLMERGMRKNRRRTPGQITVESFW"
     gene            290..1144
                     /locus_tag="VGO02_08110"
     CDS             290..1144
                     /locus_tag="VGO02_08110"
                     /inference="COORDINATES: protein motif:HMM:NF039714.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HEV7477686.1"
                     /translation="MRRALVLLAALAAGAVLSGCSSMRLAYDNADTYLLWRAGSYFDL
                     HGAAGDEFEERIDAFFDWHRANALPQYARLSEEAAKRLSDGLSPDDLVWGYDALMAQA
                     RVSLRTAATQIAPLLDRMSAEQIGHLERRLAEDNRKFAKENLRGSEAERRARRAKQMK
                     NRLEDWVGRLTQAQVERVKLYAARTPLTDEFRDRDRKRLQAEVLGMVRKREAGKRLPD
                     LAANWQKGRDPAFVTVNETWRREFYALLLDIDRSLSPAQRAKAVANFRRYAEDFAVLA
                     GQAEPRVQ"
     gene            1141..4065
                     /locus_tag="VGO02_08115"
     CDS             1141..4065
                     /locus_tag="VGO02_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEV7477687.1"
                     /translation="MNNPAPYPGIDPNTLEQTPASYAGRTRYTQLEFESILANAALGI
                     AFTRERVFYLCNPKFAEILGYEPHELIGRSGEMVYVSPESYTALGRIAVPVLSAGRQL
                     DVEWEFRRKDGSTFIARVIARAVSEENTQQGTVWIVEDISERRRHADEVARLLREQEA
                     ILGTASVGVVFVKDRRIVRCNARYEQMYGYGPGDLDGQPTRILYPDDAEYDAAALGYV
                     GFARGQPARRIGLRKRKDGSIFWNRADGRAVDPQNPQRGAVWTVEDVTEQRHAEEELQ
                     RLLAEQQALIENVSVGIAFSRKRKTVRCNRRYEEMFGYAPGEVVGRPTRDHYFTDDEY
                     QEIGRVYKDLDGGVSHSREQWLRRQDGSGFWCRVSGRAVQAGDADRGYVWLLTDITQE
                     RAAEERVQRALAEQELILDHASVGIAFVRNRFIQRCNRFLEDMVGAGPGELVGESTAV
                     LFPSADEWEAAGLRSYGNTPPGGTFEKEITLRRRDDTTFICRSRGRRIDAGDVEQEWI
                     WSYEDVTAEHQARELAAASRDALERAVAERTAELQTANARAQHLADHDALTGLPNRRL
                     LEDRLKQALALSYRNRKQTAVMFIDLDRFKTINDSLGHAVGDVLLKEVAQRLVKQLRE
                     GDTICRIGGDEFMVVLPEVKRSSHVAQVAQKIIEQLSQPAVVEERELTVTPSIGIAVF
                     PDDGRDAETLIRNADAAMYHAKEMGRANYQFFTEQMNLIAARRLALEGDLRRSLGRDE
                     MRLYYQPIVDTVSGKVAAHEALLRWQHPVRGLLSPDEFIQAAEETGLILKIGEWVLRE
                     ACMWATFIGTERKLLINVNLSARQFNDPKLPQLVSAALRESGLPAELLELEITESTAM
                     QQTDLTLGTLKKLKDLGVSIAIDDFGTGYSSLAYLKRFPVDKLKIDRSFVADIAKMKE
                     QRAIVEAIVALAHALDLEVIAEGVENEAQRKFLRGCGCEYIQGYASGKPAEADRAAEK
                     YI"
     gene            4013..>4467
                     /locus_tag="VGO02_08120"
     CDS             4013..>4467
                     /locus_tag="VGO02_08120"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEV7477688.1"
                     /translation="MPPANPPRRTARPKNISKEEDFLGGARAGLPVVLGYLGIGLAAG
                     VVERAAGMSYAEVLLISTVLYAGSAQFVVTSMLSLASPAAAIVLTVFFLNLRHLLLSA
                     ALAPALRHVPAWKNALVGLQLTDETFVVAAGKPTLSPAWMAGLNLAAWTT"
BASE COUNT          736 a         1585 c         1528 g          618 t
ORIGIN      
        1 ccagggaact cgtctaccag gatctgatca ggccgatgaa gcagttgaga tatgtcccca
       61 tcgtgagccg cgaggcggcg ccgggcgcgc tctccggccg catcccggcg gccattgccg
      121 acggccggct ggaggccgcc gcgggcgtgc gcctcgccgc cgagacctcg caggtcatgc
      181 tctgcggcaa cccgcagatg ctcaaggacg cagccgcggt gctgatggag cgcggcatgc
      241 gcaagaaccg ccgccgcact cctgggcaga tcacggtgga gagcttctgg tgaggcgcgc
      301 tcttgtgctt ctcgccgcgc tggcggccgg cgcggtcctt tcgggctgca gctcgatgcg
      361 gctcgcctac gacaacgccg acacctatct gctctggcgc gccggcagct acttcgatct
      421 gcacggcgcc gccggcgacg agttcgaaga gcgcatcgac gctttcttcg actggcatcg
      481 cgccaacgcc ctgccgcaat acgcccgcct ctcggaggag gcggcgaagc ggctgagcga
      541 cggtctgtcg cccgatgacc tggtttgggg ttacgatgcc ctgatggcgc aggcgcgggt
      601 gagcctgcgc acagcggcca cccagatcgc gccgctgctc gatcgcatga gcgccgagca
      661 gatcggccat ctcgagcggc gcctcgccga ggacaaccgc aagttcgcga aggagaatct
      721 gcggggcagc gaagcggagc gccgcgcgcg gcgcgccaag cagatgaaga accggctgga
      781 ggattgggtg gggaggctga cgcaggcaca agtcgagcgg gtgaagctct acgcggcgcg
      841 cacgccgctg accgacgaat ttcgcgaccg cgaccgcaag cgcctgcagg ccgaggtcct
      901 cggtatggtc cgcaagcggg aggcgggcaa gcgcctgccg gatctcgccg ccaactggca
      961 gaagggccgc gaccccgcct tcgtcaccgt gaacgagacc tggcgccgcg agttctacgc
     1021 gctgctcctc gacatcgacc gctcgctcag ccccgcgcag cgcgccaagg cggtggcgaa
     1081 cttccggcgc tacgccgagg atttcgcggt tctggccggc caggccgaac ctcgcgtcca
     1141 gtgaacaacc cggcccccta cccgggaatc gatccgaaca ccctggaaca gacgccggcg
     1201 agctacgccg gccgcacccg ctacacccag ctcgagttcg agtcgatcct cgccaacgcg
     1261 gcgctcggca tcgcgttcac gcgcgagcgg gtcttctacc tgtgcaaccc gaagttcgcc
     1321 gagatcctcg gctacgagcc gcacgagctg atcggccgct cgggcgagat ggtgtacgtc
     1381 tcgcccgaga gctacacggc gctcggccgc atcgccgtgc cggtcctctc cgccggccgc
     1441 cagctcgacg tcgagtggga gttccgccgc aaggacggct cgaccttcat cgcgcgcgtc
     1501 atcgcgcgcg cggtgagcga ggagaacacg cagcagggca cggtctggat cgtcgaggac
     1561 atcagcgagc ggcggcgcca cgccgacgag gtggcgcgcc tgctgcgcga gcaggaggcg
     1621 atcctcggca cggcgtcggt cggcgtggtg ttcgtcaagg accgccgcat cgtgcgctgc
     1681 aacgcacgct acgagcagat gtacggctac gggccgggcg atctcgacgg ccagccgacg
     1741 cgcattctct acccggacga cgccgagtat gacgcggcgg ccctgggcta cgtcggcttc
     1801 gctcgcggcc agccggcgcg gcgcatcggc ctgcgcaagc gcaaggacgg cagcatcttc
     1861 tggaaccgcg ccgacggccg cgcggtcgac ccgcagaacc cgcagcgcgg cgcggtgtgg
     1921 acggtggagg acgtcaccga gcagcgccac gccgaggaag agctgcagcg cttgctcgcc
     1981 gagcagcagg cgctgatcga gaacgtctcg gtcggcatcg ccttcagccg caagcgcaag
     2041 acggtgcgct gcaaccggcg ctacgaggag atgttcggct acgcgcccgg cgaggtcgtc
     2101 ggccgcccga ctcgcgacca ctactttacc gatgacgaat accaggagat cgggcgcgtc
     2161 tacaaggacc tcgatggcgg tgtgtcgcac tcgcgcgagc agtggctgcg ccgccaggac
     2221 ggctcgggtt tctggtgccg ggtcagcggc cgcgcggtgc aggccggcga cgcagatcgc
     2281 ggctacgtct ggctgctcac cgacataacc caggagcgcg ccgccgagga gcgcgtgcag
     2341 cgcgcgctcg ccgagcagga gctgatcctc gaccacgcct cggtcggcat cgccttcgtg
     2401 cgcaaccgct tcatccagcg ctgcaaccgc ttcctcgagg acatggtggg cgccggcccg
     2461 ggcgagctgg tgggcgaaag caccgcggtc ctcttcccaa gcgcggacga gtgggaggcg
     2521 gcgggcctgc gctcctacgg caacacgccg ccgggcggca ccttcgagaa agagataacg
     2581 ctgcgccggc gcgacgacac caccttcatc tgccgctcgc gcggccggcg catcgacgcc
     2641 ggagacgtcg agcaggagtg gatctggagc tacgaggacg tcaccgccga gcaccaggcg
     2701 cgcgagctcg ccgccgcgtc gcgcgacgcg ctggagcgcg cggtcgccga gcgcaccgcc
     2761 gagctgcaga cggccaacgc gcgcgcccag cacctcgccg accacgacgc gctcaccggc
     2821 ctgcccaacc ggcggctgct ggaagaccgg ctgaagcagg cgctcgcgct cagctaccgc
     2881 aaccgcaagc agaccgcggt gatgttcatc gacctcgacc gcttcaagac catcaacgac
     2941 tcgctcggcc acgcggtggg cgacgtcctg ctgaaggaag tcgcgcagcg cctggtaaag
     3001 cagctgcgcg agggcgacac catctgccgc atcggcggcg acgaattcat ggtggtgctg
     3061 cccgaggtca agcgctcctc gcacgtcgcg caggtggcgc agaagatcat cgagcagctg
     3121 tcgcagccgg cggtggtcga ggagcgcgag ctcaccgtga cgccgtcgat cggcatcgct
     3181 gtgttccccg acgacggccg cgacgccgag acgctgatcc gcaacgccga cgcggcgatg
     3241 taccacgcca aggagatggg gcgcgccaac taccagttct tcaccgagca gatgaacctg
     3301 atcgcggcgc ggcgcctcgc gctggagggc gacctgcgcc gctcgctggg gcgcgacgag
     3361 atgcggctgt actaccagcc gatcgtcgac acggtgagcg gcaaggtcgc cgcgcacgag
     3421 gcgctgctgc gctggcagca cccggtgcgc ggcctgctct cgcccgacga attcatccag
     3481 gcggccgagg aaaccggcct gatcctgaag atcggcgagt gggtgctgcg cgaggcgtgc
     3541 atgtgggcca ccttcatcgg caccgagcgc aagctgctga tcaacgtcaa cctgtcggcg
     3601 cgccagttca acgatcccaa gctgccgcag ctcgtctcgg ccgccctgcg cgagagcggc
     3661 ctgcccgcgg agctgctcga gctcgagatc accgagtcga ccgcgatgca gcagaccgac
     3721 ctcaccctcg gcacgctgaa gaagctgaag gacctgggcg tgtccatcgc catcgacgac
     3781 ttcggcaccg gctactcgag cctggcttac ctcaagcgct tcccggtcga caagctgaag
     3841 atcgaccgct cgttcgtcgc cgacatcgcc aagatgaagg agcagcgcgc catcgtcgag
     3901 gcgatcgtcg cgctggccca cgccctcgac ctcgaggtga tcgccgaggg cgtcgagaac
     3961 gaggcgcagc gcaagttcct gcgcggctgc ggctgcgagt acatccaggg ctatgcctcc
     4021 ggcaaacccg ccgaggcgga ccgcgcggcc gaaaaatata tctaaagaag aagacttcct
     4081 cggcggcgcc cgggccggcc tgccggtggt gctcggctac ctcggcatag gcctcgccgc
     4141 cggcgtggtg gagcgcgccg ccggcatgtc gtacgccgag gtgctcctca tctccaccgt
     4201 gctctacgcc ggctcggcgc agttcgtagt gaccagcatg ctgagcctcg ccagccccgc
     4261 cgcggcgatc gtcctcaccg tcttcttcct caacctgcgc catctcctgc tctcggccgc
     4321 gctcgccccg gcgctgcgcc acgtcccggc gtggaaaaac gcgctcgtcg ggctgcagct
     4381 caccgacgag actttcgtgg tcgccgcagg caagccaacg ctgtcaccgg catggatggc
     4441 cgggctgaat ctcgctgcgt ggaccac
//
LOCUS       DASYJA010000369         3896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2413789, whole genome shotgun sequence.
ACCESSION   DASYJA010000369 DASYJA010000000
VERSION     DASYJA010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2413789"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VGO02_08125"
     CDS             complement(<1..306)
                     /locus_tag="VGO02_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477689.1"
                     /translation="MARIICGHFAESVKADAALQALLQAGFARDRIDSFYTPPPGQHA
                     LHPLGGDAHSDAGARKAGAGAAIGAAVGAAIGALIGFGAIRAGIIPPSDLTPVAVLFA
                     "
     gene            complement(308..1690)
                     /locus_tag="VGO02_08130"
     CDS             complement(308..1690)
                     /locus_tag="VGO02_08130"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEV7477690.1"
                     /translation="MGPAIALRLRQHLTLTPQVQQALRLLQMSALEFAQEMDQALQEN
                     PFLEEKEPAPEAADAPAAEAGPGIDDLAPAPEAPAASSSDREQEEWSGAADTAHSLQD
                     HLREQLMISQMGSRDRALAHMIIDSLDDDGYFKIGFDELAALVPAEHDVRAEDFAAAL
                     RLVQSLDPAGVGARSLEECLCLQIEALPDDTPARDTALAICHGHLALLGNREWARLQH
                     AVGCDEAQLHRARNLIKSLDPRPGHRFGTSEARYVIPDVIVRKAREKWSASINPASLP
                     RVRLNRAYAEAIASRGQSNQSLSRQLQEARWLLRSIEQRFVTIQRVADAIVARQRGFF
                     EYGEVAMKPLTLKLIAGELGLHESTVCRVTNSKYMSTPRGLFEFKHFFSRKLATDSGG
                     AASATAVRAVMKEMIAAEDPRAPLSDAELARLLAQQGLRVARRTITKYRALMRLPSVD
                     VRRLELRRGA"
     gene            1828..>3896
                     /gene="ligD"
                     /locus_tag="VGO02_08135"
     CDS             1828..>3896
                     /gene="ligD"
                     /locus_tag="VGO02_08135"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEV7477691.1"
                     /translation="MTERLERYRERRDFSRTPEPEGARSRAKGTRELRYYIQRHDARR
                     LHYDFRLELDGVLKSWAVPKGPSLDPGVKRLAVEVEDHPLEYGEFEGLIPEKQYGAGD
                     VLLWDKGTWIAEDRDPEAALRKGRLHFQLKGVKLQGRWVLFRTKDKQWILRKREDDAA
                     RAGEITQEKPESVKKAKIKKGKARRTGYLPPFIPPQLATLVEEPPRTGSWVYEVKYDG
                     YRLLAKLTKDGVHLFTRAGNDWTSKLPRLAAELKKLKLDGTWLDGEIVVPAQDGRASF
                     QQLQNAFDAGNDARIVYFVFDAPFLGGEDLRSLPLLERKKRLRESLKGNKVVRYSEHL
                     SADPDEALAHACKLKLEGLIAKRTGSVYVSGRSRDWLKLKCRQRQDFVIGGYTAPRGS
                     RQGFGALLVGYHEPGGKLRYAGKVGTGFDDKMLSSLKEKFSKLESKDAPFENPPRERG
                     VTWLKPSLVAEVEYAERTEEGLLRQAAFMGLRRDLPAKQVAEEKPVKLVKITHPDRLI
                     WPKLKVTKAAFAGYVEEIGDWLLPHLAKRPLSLVRCPDGAAGKCFYQRHPAAGLKLKT
                     IGDYVYLETLNDVVAAVQYGAVEFHTWGASVPDVARPDRLTLDLDPDAGLPWGRLLDA
                     TRLTKTLLDQLKLVSFLKTTGGKGLHIVVPIEPKLGWDEVKEFTRRIALFLEKAQPDL
                     FLANMSKA"
BASE COUNT          647 a         1281 c         1379 g          589 t
ORIGIN      
        1 ggcgaacagc acggcgaccg gggtcaaatc cgagggcggg atgatgccgg cgcggatggc
       61 gccgaaaccg atcagggcgc cgatcgccgc gccgacggcg gcgccgatcg cggcgcccgc
      121 tccggccttg cgcgcgccgg cgtcgctgtg cgcgtcgccg cccagcggat gcagcgcgtg
      181 ctggcccggc ggcggggtat agaacgaatc gatccggtcg cgcgcaaagc ctgcctgcag
      241 gagggcctgc agcgcggcgt ctgccttgac gctctcggcg aaatgcccgc agatgatgcg
      301 cgccacgtca cgccccccgg cgcagctcga ggcggcgcac gtccacgctc ggcagccgca
      361 tgagcgcgcg gtacttggtg atggtgcgcc gcgccacgcg caggccctgc tgcgcgagca
      421 ggcgcgcgag ctcggcgtcg gacagcggcg cgcgcgggtc ctcggccgcg atcatctcct
      481 tcatcaccgc gcgcaccgct gtggccgagg cggcgccgcc cgagtcggtg gcgagcttgc
      541 gcgagaagaa gtgcttgaac tcgaacaggc cgcgcggcgt cgacatgtac ttgctgttgg
      601 tgacgcggca cacggtggac tcgtgcaggc cgagctcgcc ggcgatcagc ttgagggtca
      661 ggggcttcat cgccacttcg ccgtactcga agaagccgcg ctggcgcgcg acgatggcgt
      721 cggcgacgcg ctgaatggtg acgaagcgct gctcgatcga gcgcagcagc cagcgcgcct
      781 cctgcagctg gcgcgaaagc gactggttgg actggccgcg gctcgcgatc gcctcggcgt
      841 aggcgcggtt caggcgcacg cgcggcagcg aggccgggtt gatcgaggcg ctccacttct
      901 cgcgcgcctt gcgcacgatg acgtcgggga tgacgtagcg cgcctcggac gtgccgaagc
      961 ggtgccccgg ccgcggatcg agcgacttga tcaggttgcg cgcgcggtgc agctgcgcct
     1021 cgtcgcagcc gaccgcgtgc tgcagccgcg cccattcgcg gttgccgagg agggcgaggt
     1081 ggccgtggca gatggcgagc gcggtgtcgc gcgccggcgt gtcgtcgggc agcgcctcga
     1141 tctgcaggca caggcactcc tcgagcgagc gggcgccgac gccggcggga tccaggcttt
     1201 gcaccaggcg cagcgccgca gcgaagtcct cggcgcgcac gtcgtgctcg gcgggcacca
     1261 gcgccgccag ctcgtcgaag ccgatcttga aatagccgtc gtcgtccagg ctgtcgatga
     1321 tcatgtgggc gagcgcgcgg tcgcggcttc ccatctgcga gatcatcagc tgctcgcgca
     1381 ggtggtcctg caggctgtgc gcggtgtcgg cggcacctga ccactcctcc tgctcgcgat
     1441 cagaggagct ggccgcgggc gcttcgggcg cgggggcgag gtcgtcgatg ccggggccgg
     1501 cttccgcggc gggcgcgtcg gcggcctcgg gcgcgggctc cttctcttcc aggaacgggt
     1561 tttcctgcag cgcctggtcc atttcctggg cgaattcgag ggcggacatc tgcagcaggc
     1621 gcaaagcctg ctggacttgc ggggtaaggg tgaggtgctg cctgaggcgc agtgcaattg
     1681 ccgggcccat gttcttcctt tcagcccccc gatctgcaaa ggccatgcca gtggctttag
     1741 gcctgtggac gcaagcaatc tcctgtaata gtttccggcg ccggtagcaa ttaccgtacg
     1801 ggcccgcggt ttgctgcctg cagtcgcatg accgaacgcc tggagcgcta tcgcgagagg
     1861 cgcgatttct cgcgcacgcc cgaaccggag ggcgcgagaa gccgcgccaa aggcacgcgt
     1921 gaactgcgct actacatcca gcgccatgac gcgcgccgcc tgcactatga ctttcgcctc
     1981 gagctcgacg gcgtgctgaa gagctgggcg gtgccgaagg ggccgagcct ggaccccggc
     2041 gtcaagcgcc tggcggtcga agtcgaggac catccgctgg aatacggcga gttcgagggc
     2101 ctgatccccg aaaagcagta cggcgcgggc gacgtcctgc tgtgggacaa gggcacctgg
     2161 atcgccgagg accgcgaccc ggaagcggcg ctgcgcaagg ggcggctgca cttccagctg
     2221 aaaggcgtga agcttcaggg ccgctgggtt ctattcagga caaaggacaa gcaatggatc
     2281 ctgcgcaagc gcgaggacga cgcggccagg gccggcgaaa tcacgcagga gaaacccgag
     2341 agcgtaaaga aagcgaagat caagaaaggg aaagcgcgcc gcaccggcta cctgccgccg
     2401 ttcattcccc cccagctcgc cactcttgtc gaagagccgc cgcgcaccgg cagctgggtc
     2461 tacgaggtga agtacgacgg ctaccggctg ctcgccaagt tgaccaagga cggcgtgcac
     2521 ctgttcacgc gcgccggaaa cgactggacc tccaagctgc cgcgcctcgc ggcggagctg
     2581 aagaagctga agctcgacgg cacctggctc gatggcgaga tcgtggtgcc ggcgcaagac
     2641 ggccgcgcca gcttccagca gctgcagaac gcattcgacg ccggcaacga tgcgcggatc
     2701 gtttactttg tcttcgacgc gccttttctc gggggcgagg acctgcgctc cctccctctg
     2761 ctcgagcgca agaagcgtct gcgagagtcc ctgaagggaa ataaggtcgt gagatatagc
     2821 gagcaccttt cggccgaccc ggacgaagcg ctcgcgcatg cctgcaagct caagctagaa
     2881 ggtctcatcg cgaagcgcac cgggagcgtc tacgtgtccg gacgcagccg cgactggctg
     2941 aagctcaagt gccgccagcg gcaggacttc gtcatcggcg ggtacactgc gccgcgtggc
     3001 tcgcggcagg gcttcggcgc gctcctggtc ggctaccacg agcccggcgg caagctgcgc
     3061 tacgcaggca aggtcggtac tggcttcgac gacaaaatgc tgtcgagcct gaaggagaag
     3121 ttctcgaagc tcgagagcaa agatgcgcct ttcgaaaatc cgccgcgcga gcggggcgtt
     3181 acctggctca agccgtcgct cgtcgccgag gtcgagtacg ccgagcgcac cgaggagggc
     3241 ctgctgcgcc aggcggcttt catgggcctg cgccgcgact tgccggcaaa gcaggtggca
     3301 gaggagaagc cggtgaagct ggtcaagatc acccaccccg accggctcat ctggccgaag
     3361 ctcaaggtga ccaaggccgc cttcgccggc tatgtcgagg agatcggcga ctggctgctt
     3421 ccgcacctcg cgaaacggcc gctatcgctc gtgcgctgcc ccgacggcgc cgcaggcaag
     3481 tgcttctatc agcgccatcc ggccgccggg ctgaagctga agaccatagg ggactacgtc
     3541 tatctggaga cgctgaacga cgtcgtcgcg gccgtgcagt acggtgcggt ggagttccat
     3601 acgtggggcg ccagcgttcc cgacgtggcc cgccccgatc gcctcacgct cgacctcgac
     3661 ccggatgccg gtctgccgtg gggcaggctg ctcgacgcca cccggctgac gaagacgctc
     3721 ctcgaccagc tgaagctcgt ctcgttcctc aagaccaccg gcggcaaggg actgcacatc
     3781 gtggtgccga tcgagccgaa gctgggctgg gacgaggtca aggagttcac gcgccgcatc
     3841 gcgctgttcc tggagaaagc gcagccggat ctgttcctgg ccaacatgtc gaaggc
//
LOCUS       DASYJA010000370         2888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1688992, whole genome shotgun sequence.
ACCESSION   DASYJA010000370 DASYJA010000000
VERSION     DASYJA010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1688992"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VGO02_08140"
     CDS             complement(<1..500)
                     /locus_tag="VGO02_08140"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7477692.1"
                     /translation="MRSPLLAALALVCAVAGAEEGAQIPPFITTPDEVVERMLRLAGT
                     GPADVVIDLGSGDGRIVIAAAQKFGARGIGIELDPRLVEKSRDSARVAEVAERVSFIE
                     GDVLATDISQASVVTVYLLPFLIDKLQPRFLELKPGTRVVSHAFGMVGWKPDRAETMR
                     LTKPHPG"
     gene            complement(500..1033)
                     /locus_tag="VGO02_08145"
     CDS             complement(500..1033)
                     /locus_tag="VGO02_08145"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEV7477693.1"
                     /translation="MLAALLAASAGPAWAQGIIKSLPTGPVVPAQVPALKPWSGGPTP
                     ALDLPGLDGAEHRLRDYRGKVVLVNFWATWCTPCRDEMPSIERLRASLGGRPFAVLAV
                     NVGETERAARAFLQTMPVGFTVLLDRELKASRTWGARVLPASYLVGPDGAIRYSYFGE
                     LDWAKDSVVQAIAQMMA"
     gene            complement(1115..1330)
                     /locus_tag="VGO02_08150"
     CDS             complement(1115..1330)
                     /locus_tag="VGO02_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477694.1"
                     /translation="MKKLLVALIAAAFAATAFAQAPKSTEPATKTEKSDKKAKAKGEK
                     KAKSTMAKSDKKSTDKKAGGMEKKDSK"
     gene            1501..2073
                     /locus_tag="VGO02_08155"
     CDS             1501..2073
                     /locus_tag="VGO02_08155"
                     /inference="COORDINATES: protein motif:HMM:NF016627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEV7477695.1"
                     /translation="MELQDFMAGLPGGDLWVFGYGSLIWSPGFSYKEKRVGRAHGYHR
                     ALCILSTRYRGTQRRPGLVMGLCRGGSCWGMAFRVDAARVRRVLGRLWSREMLRRVYE
                     PRLIRVQTRGGRRVHALAFLADPAHPAFVRELDLHGRARMVAQGIGQRGHCIDYIRNT
                     IEHMHELGVRDPHLERILHAALALRENGAK"
     gene            2099..>2888
                     /locus_tag="VGO02_08160"
     CDS             2099..>2888
                     /locus_tag="VGO02_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7477696.1"
                     /translation="MIPLWPARLHHLRRDSPQPERLAKFYGELLGDRVEPLGGETWLV
                     AGSERRLVIGKGAAGAVPYFALQMQDLSQLAAYRHALTGTEDFHSTLLTEGAFALTDP
                     DGRRVAFGLPRVGLSQGGMPGRLQHFVCATKQLPEMVDFYRGLGMVESDRVLDKEELA
                     ASFLRSDPEHHSFAAFRAPESRPDHHCYETHGWLDIRDWADRMGKLGIALWWGPGRHG
                     PGNNLFFMVEDPDGYKVEFSAELELIPKEVPYRTWPHEQRTLNLW"
BASE COUNT          436 a          994 c          986 g          472 t
ORIGIN      
        1 ccgggatgcg gcttggtcag gcgcatcgtt tcggcgcggt cgggcttcca gccgaccatg
       61 ccgaaggcat gcgatacgac ccgcgtgccg ggcttgagct cgagaaagcg cggctgcagc
      121 ttgtcgatga ggaagggcag caggtagacg gtgactacgc tcgcctgcga gatgtcggtg
      181 gcgaggacat cgccctcgat gaatgagacg cgctcggcga cctcggcgac gcgcgcgctg
      241 tcgcgtgact tttccaccag cctggggtcg agctcgatgc cgatgccgcg cgcgccgaac
      301 ttttgcgccg ctgcgatgac gatgcgcccg tcgcccgagc cgaggtcgat cacgacgtcg
      361 gcgggcccgg tgccggcgag gcgcagcatg cgctcgacca cctcgtccgg ggttgtgatg
      421 aacggcggga tctgcgcgcc ctcctccgcg ccggcgacag cgcagacgag cgcgagcgct
      481 gcaagcagcg gcgagcgcat cacgccatca tctgcgcgat cgcctgcacc acgctgtcct
      541 tggcccaatc gagctcgccg aagtagctgt agcggatcgc gccgtcgggg ccgacgaggt
      601 agctcgccgg caggacgcgc gcgccccatg tgcggctcgc cttgagctca cggtcgagca
      661 ggacggtgaa gccgacgggc atcgtctgca ggaaggcgcg cgccgcgcgc tcggtctcgc
      721 cgacgttcac cgccagcacg gcgaacggcc ggccgccgag cgaagcgcgc agccgctcga
      781 tgctcggcat ctcgtcgcgg cagggcgtgc accaggtagc ccagaaattc accagcacca
      841 ccttgccgcg atagtcgcgc agccggtgct cggcgccgtc gaggccgggg agatcgagcg
      901 ccggggtcgg cccgccgctc cagggcttga gcgcaggcac ctgcgcgggc accaccggac
      961 ccgtgggcag ggacttgatg atgccctgcg cccacgccgg gccggcggac gcggcgagca
     1021 gggcggccag cattcggcgt cgcgtcagca cgcgcgaact gtaccgcaaa aaaaaagccc
     1081 ggcgcgaagc cgggctgcgg ttggtgcgaa agacttactt cgagtctttc ttctccatgc
     1141 cgccggcttt cttgtcggtc gacttcttgt ccgactttgc catggtggac ttggctttct
     1201 tctcgccctt ggccttggct ttcttgtccg atttctcggt cttggtcgcc ggctcggtcg
     1261 acttcggggc ctgggcaaac gcggtggccg cgaaggcggc agcaatcagc gcaacgagca
     1321 gcttcttcat gtggactgtc tcctagagaa tggatgatcc aatagcggat ctggttcgaa
     1381 taacgcctca gcggcccggg cgttgacagg catcctaacc ggggggcatt acgccaattg
     1441 gagacaatcc ccggccgttg ccgaacggcg acaggccagt cctataatcg gcgggcaaga
     1501 atggagcttc aggatttcat ggccgggctg ccgggcggcg acctctgggt cttcggctac
     1561 ggctcgctca tctggtcgcc cggcttcagc tacaaggaaa agcgcgtcgg ccgggcgcac
     1621 ggctaccacc gcgccctctg cattctttcg acccgctacc gcggcacgca gcgcaggccc
     1681 ggcctggtga tgggcctgtg ccgcggcggc tcctgctggg gcatggcgtt ccgcgtcgac
     1741 gccgcgcgcg tgcgccgcgt gctcggccgg ctgtggagcc gcgagatgct gcgccgcgtc
     1801 tacgagccgc gcttgatccg ggtgcagact cgcggcgggc gccgcgtgca cgcgctcgcc
     1861 ttcctcgccg atccggcgca tccggctttc gtgcgcgagc tcgacctgca cggccgtgcg
     1921 cgcatggtgg cgcagggcat cggccagcgc ggccattgca tcgactacat ccgcaacacg
     1981 atcgagcaca tgcacgagct cggcgtgcgc gacccgcacc tcgagcgcat cctgcacgcc
     2041 gcgctcgctt tgcgggagaa tggagcgaag tgatgcctcg cgaacatcgt attcgcccat
     2101 gatccccctc tggcccgcac ggctccacca cctgcgccgc gacagcccgc agcccgagcg
     2161 cctggcgaag ttctacggcg agctgctcgg cgaccgcgtc gagccgctcg gcggcgagac
     2221 ctggctggtg gcgggcagcg agcggcgcct ggtgatcggc aagggggccg ccggcgcggt
     2281 gccctatttc gcgctgcaga tgcaggacct gtcccagctg gcggcgtatc gtcacgcgct
     2341 gacagggacc gaagacttcc attcgaccct gctgaccgaa ggcgccttcg cgctcaccga
     2401 tcccgacggc cgccgggttg cctttggtct tcccagagtt ggtctttctc aaggggggat
     2461 gccggggcgt ctccagcact tcgtgtgcgc gaccaagcaa ctgccggaga tggtcgattt
     2521 ctaccgcggc ctgggaatgg tggagtccga ccgcgtgctc gacaaggagg agctcgccgc
     2581 gagcttcctg cgctccgatc ccgagcacca cagcttcgcc gccttccgcg cgcccgagtc
     2641 gcgccccgac caccactgct acgagaccca cggctggctc gacatccgcg actgggccga
     2701 ccgcatgggc aagctcggca tcgcgctgtg gtggggaccc gggcgccacg ggccgggaaa
     2761 caacctgttc ttcatggtcg aggatcccga cggctataag gtcgagttct cggccgagct
     2821 cgagctgatt ccgaaggaag ttccctatcg cacctggccc cacgagcagc gcacgctcaa
     2881 cctctggg
//
LOCUS       DASYJA010000371         1659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_873833, whole genome shotgun sequence.
ACCESSION   DASYJA010000371 DASYJA010000000
VERSION     DASYJA010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873833"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VGO02_08165"
     CDS             complement(<1..443)
                     /locus_tag="VGO02_08165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEV7477697.1"
                     /translation="MRKSILTLSVAAALATPGLAAAQAAAPAAAPAPSPVTGNMTIAS
                     DYRFRGISQTYLGPAIQGGIDYAHPSGFYIGNWNSNVASQVYSGGSGIEMDFYGGYKK
                     AFGDIGLDVGFIYYYYHNAEFLSKKFNNQEIYIGGSWKWLSAKLN"
     gene            631..873
                     /locus_tag="VGO02_08170"
     CDS             631..873
                     /locus_tag="VGO02_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEV7477698.1"
                     /translation="MIDRRLLDDLRSRIEAALHDSPAQDIERNLRALLASWFDRLDLV
                     LREDFEVQKKLLERAQMKLAELEARVAELEASKPPR"
     gene            927..>1659
                     /locus_tag="VGO02_08175"
     CDS             927..>1659
                     /locus_tag="VGO02_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEV7477699.1"
                     /translation="MSVATVASRALAGIDAPEVTVEVHLGPGLPAFHIVGLPDAEVRE
                     ARDRVRAALNHAHFEFPARRMTVNLAPAELPKDSTRFDLPIALGILAASGQLAAAALA
                     GQEFAGELSLTGDLRPVRGALAMALSARTAGRAFVLPEGNAPQAALAEGARILPARTL
                     LEVVAHLAGESPLAQYAGSGASAAPVYPDFGDVKGQQQVKRALEVAAAGGHSVLMIGP
                     PGTGKSMLAKRLATILPPLTLEEALE"
BASE COUNT          289 a          515 c          549 g          306 t
ORIGIN      
        1 tagttcagct tggcggacag ccacttccag gatccaccga tgtagatttc ctggttgttg
       61 aacttcttcg agaggaattc ggcgttgtgg tagtagtagt agatgaagcc gacgtcgagg
      121 ccgatgtcgc cgaaggcttt cttgtagccg ccgtagaaat ccatctcgat gcccgagccg
      181 ccgctgtata cctggctggc gacgttcgag ttccagttgc cgatgtagaa gccgctcgga
      241 tgggcgtagt cgatgccgcc ctgtatggca ggtcccaggt aggtttggga aatgccgcgg
      301 aaccggtagt cgctggcgat cgtcatgttg ccggtgacgg gcgacggggc cggcgcggcg
      361 gcgggggcgg cggcctgcgc tgccgcgagg ccgggcgtag caagagccgc tgctacggac
      421 agcgtgagaa tggacttacg catcgaaaat ctccctaagg gttggaaatt aagtattgca
      481 gtcactcaat tagcatctcc tgtgccacac aaatttacat tgatctttca atgaattagg
      541 ataatcggaa cgggacgatg caccgatgga gtgcgccgcc tctcggtctg cgcccggagt
      601 tggtgcggaa gcgagtgata ccttcgcccc atgatcgatc gccgcctgct ggatgacctg
      661 cgcagccgta tcgaggctgc actgcacgac tctcccgccc aagacatcga gcggaacctg
      721 cgcgccttgc tcgcgtcgtg gttcgaccgc ctggatctcg tgctgcgcga ggacttcgag
      781 gtgcaaaaga agctgctcga gcgcgcgcag atgaagctcg ccgagctcga agcacgggtc
      841 gcggagctcg aagcgtccaa accgccccgg taaacgttcc ttgctcagcc gcgtttctcg
      901 ttccagagga tcggaaaggg gaccgcttgt cagtcgccac ggtggcgagc cgcgcgctcg
      961 cgggtattga tgcgcccgaa gtaactgtag aagttcacct cgggccggga ctacccgcat
     1021 ttcatatcgt cgggctgccc gacgccgaag tgcgcgaagc gcgcgatcga gtgcgtgcgg
     1081 cgctcaacca tgcgcatttc gagtttccgg cccggcgcat gacggtcaac ctcgcgccgg
     1141 ccgagctgcc gaaagactcg acccgtttcg acctgccgat tgcgctcggc atcctcgcag
     1201 cgagcggcca gctcgccgcc gccgcgctcg ccggccagga attcgcgggt gagctgtctc
     1261 tcaccggaga tctgcggccg gtgcgcggcg cgctggcgat ggcgctttcg gcgcgcacgg
     1321 cgggccgcgc tttcgtgctg ccagagggca atgcgccgca ggccgcgctt gccgaaggcg
     1381 cgcgcattct tcccgcccgc acgctgctcg aagtcgtcgc tcacctggcc ggggaaagcc
     1441 cgcttgctca gtatgccggc agcggtgcca gcgcggcacc cgtctatccg gacttcggcg
     1501 atgtaaaagg acagcagcag gtcaaacgcg ccctcgaggt cgcggccgcc ggagggcaca
     1561 gcgtgctgat gatcggcccg ccgggcaccg gcaagtcgat gctcgcgaaa cggcttgcga
     1621 ccattctgcc gccgctcacg cttgaggaag cgctggaga
//
LOCUS       DASYJA010000372         3738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_691071, whole genome shotgun sequence.
ACCESSION   DASYJA010000372 DASYJA010000000
VERSION     DASYJA010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691071"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VGO02_08180"
     CDS             complement(<1..963)
                     /locus_tag="VGO02_08180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7477700.1"
                     /translation="MADSVFHKDFKSMPWWWEWWHPSNELSQDPPRKTDVLVVGAGYG
                     GLSTALELARSGVEVTVLERGEFGVGASTRNGGGVSGGTTLGKGFSGKGVKSDPEEWK
                     KVMARMLTDAADSLAQVQTVIEREGIECHWRMSGRFSGAFTPRHYAEQAAKVGTYNAS
                     AGLGTYMIPREQQREEIASDYYYGGMVVERTGQLHPALYYGGLLKAASRAGAKLCAQC
                     DAEKIERKPGGFTVLTNKGPIEAREVVIATNGYTGDLTPTLKRRLIPVASHIIATEEL
                     PEDLVKSLIPKGRVVSDTKRVLCYYRQSPDSKRVIFGGRARFTQV"
     gene            complement(976..1755)
                     /locus_tag="VGO02_08185"
     CDS             complement(976..1755)
                     /locus_tag="VGO02_08185"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7477701.1"
                     /translation="MSRRLLVAACGLIYFFLMLPLLVVFPISLSSAPYMQFPPPGLSW
                     QWYERYLEDPQWIDATWRSLYIGGATAVLALALGVPLAFSLVRGRFPGRLLIDRLALA
                     PIVVPTIILSVAVYGLFAKLKLIGAWYGLVVAHTVLALPFVVLVMTAGLRDFDRGLEQ
                     AAEGLGASRISTLWRITLPLLRPSLVSAGLLAFLSSFDELVVALFLAGPNMTLPKKMF
                     DNIMMEIDPTIAAVSVMQILLVTLVMVLIGRFGRGLRGSAR"
     gene            complement(1752..2561)
                     /locus_tag="VGO02_08190"
     CDS             complement(1752..2561)
                     /locus_tag="VGO02_08190"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7477702.1"
                     /translation="MSARGFWPALPALILLLAFFLFPVVRMLGFSVEAGTLDWYAKAL
                     GEGLYLQVFWNTFEIALLVTGVCLLLGYPLGFLIATTTPTWATLGFIFVLLPLWTSVL
                     VRTYAWMVLLGRNGVFNRWLIDAGIISDPLPLLHNFTGVLIGMVHVLLPYMVLPIYGA
                     VRRLDPAIVSAAEGLGASNWRIFWRIYVPLTLNGIFAGSVLVFVLSLGFFITPALLGG
                     GRVMMIAVLIEQQVRETLNWPFAAALSAVLLAFTFAVYAVAQRFTRAETAS"
     gene            complement(2676..3680)
                     /locus_tag="VGO02_08195"
     CDS             complement(2676..3680)
                     /locus_tag="VGO02_08195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7477703.1"
                     /translation="MTKRFGEVLALDGVSLAVAEGELLTILGPSGSGKTTLLKVVAGF
                     ETPDAGTVRVDGAEITALPPARRDIGMVFQNYALFPHLTVKGNVAFPLEMRNVARAEI
                     ERRVGEALALVELAGYEARLPRQLSGGQQQRVALARAIVFNPRLLLLDEPFGALDRKL
                     RETMQLEVRRLQRRLGLTTIFITHDQEEALVLSDRVAVMNKGTIQQIATTTEIYERPA
                     NDFVADFVGESNIFHGTSTEPGVITLSGGRRLLVNGAARIGDRLGVLMRPERFAASGV
                     NTFSGEVLEAVYLGASFKLRLACADGLELLVRQPARGVLPAAGQHIEVGIDPDSIHVF
                     "
BASE COUNT          683 a         1266 c         1253 g          536 t
ORIGIN      
        1 gacctgggtg aagcgggcgc ggccgccgaa gatcacgcgc ttgctgtcgg gcgattgccg
       61 gtaatagcac agcacccgct tggtatcgct gacgacgcgg cctttcggga tcaggctctt
      121 caccaggtcc tcgggcagtt cctcggtggc gatgatgtgg ctcgccaccg gtatcagccg
      181 ccgcttgaga gtcggcgtga gatcgccggt gtaaccattc gtggcgatca cgacctcgcg
      241 cgcctcgatc ggccccttgt tggtaagcac ggtgaaacca cccggcttgc gctcgatctt
      301 ttccgcgtcg cattgcgcgc agagtttcgc gccggcgcgg ctcgcggcct tgagcaggcc
      361 gccgtaatag agcgcgggat ggagctggcc ggtgcgctcc acgaccatgc cgccgtaata
      421 atagtcggag gcgatctctt cgcgctgctg ctcgcgcggg atcatgtagg tgccgagacc
      481 ggcgctcgca ttgtaggtgc cgaccttggc ggcctgctcg gcgtagtggc gcggcgtgaa
      541 ggcgccgctg aagcggccgc tcatgcgcca atggcactcg atgccttcac gctcgatcac
      601 cgtctgcacc tgggccagcg aatcggcggc gtcggtcagc atccgcgcca tcaccttctt
      661 ccattcctcg ggatcgctct tgacgccctt gccggagaag cccttgccca gcgtcgtgcc
      721 gccgctcacg ccgccgccgt tgcgcgtcga ggcgccgacg ccgaactcgc cgcgctccag
      781 caccgtcacc tcgacacccg agcgcgccag ctcgagcgcc gtcgacagcc cgccataacc
      841 tgcgcccacc accagcacgt cggtcttgcg cggcggatcc tgcgacagct cgttgctggg
      901 atgccaccat tcccaccacc acggcatcga cttgaaatcc ttgtggaaca cgctgtcagc
      961 catgacgccc tctccttaac gcgcgctgcc gcggaggccc cggccgaagc gtccgatcag
     1021 gaccatgacc agggtcacca gcaggatctg catcaccgac accgcggcga tggtgggatc
     1081 gatttccatc atgatgttgt cgaacatctt cttgggcagg gtcatgttgg ggccggccag
     1141 gaacagcgcc accaccagct cgtcgaatga actgaggaaa gccagcaggc cggccgagac
     1201 caggctcggc cgcagcagcg gcagggtgat gcgccagagc gtgctgatgc ggctggcgcc
     1261 gagcccctcc gccgcctgct cgagcccgcg atcgaagtcg cgcaaacccg ccgtcatcac
     1321 cagcaccacg aagggcagcg ccagcacggt gtgggccacg accagcccgt accacgcgcc
     1381 gatcagcttc agcttggcga acagaccgta gaccgcgacc gagaggatga tggtcggcac
     1441 gacgatgggc gccagcgcca gccggtcgat cagcaggcga ccggggaacc tgccgcgcac
     1501 caggctgaag gcgagcggca cgcccagcgc cagcgccagc accgcggtcg ccccgccgat
     1561 atagagcgag cgccaggtgg catcgatcca ttgcggatcc tcgagatagc gctcgtacca
     1621 ctgccacgag agaccgggcg gcgggaactg catgtagggc gcggagctga gcgagatcgg
     1681 gaacaccacc agcaacggca gcatcaggaa aaagtagatc agcccgcagg ccgcgacgag
     1741 caggcggcgg ctcatgatgc ggtctccgcc cgcgtgaagc gctgcgccac cgcatagacg
     1801 gcgaaggtga aggcgagcag cacggccgag agcgccgcgg caaacggcca gttcagggtt
     1861 tcgcgcacct gctgctcgat caggacggcg atcatcatca cccggccgcc gcccagcagg
     1921 gcgggcgtga tgaagaagcc gagcgacagc acgaagacca gcaccgagcc ggcaaagatg
     1981 ccgttcaagg tgagcggcac gtagatgcgc cagaagatgc gccagttcga ggcgccgagc
     2041 ccctcggccg cggagacgat ggcgggatcg agccggcgca ccgcgccgta gatcggcagc
     2101 accatgtagg gcagcaggac gtgcaccatg ccgatcagca cgccggtgaa attgtgcaac
     2161 agcggcagcg ggtcgctgat gatgccggca tcgatcagcc agcgattgaa cacaccgttg
     2221 cggcccagca gcaccatcca ggcgtaagtg cgcaccagga cggaggtcca cagcggcaac
     2281 agcacgaaga tgaaaccgag cgtcgcccag gtgggtgtcg tggtggcgat cagaaagccc
     2341 agcggataac ccaacagtag gcagaccccg gtgaccagca gggcgatctc gaaggtgttc
     2401 cagaacacct ggagataaag cccttcgccc agcgccttgg cataccagtc gagcgtaccc
     2461 gcctcgacgc tgaaccccag catgcgcacg accgggaaga ggaagaaggc gagcaggagg
     2521 atgagggcgg gcagcgcggg ccagaagcca cgcgcgctca tcggacgaat tccctgtcat
     2581 cctgagcgta gcgaaggatc tcatcgccgc tggccaaggg catgagatcc ttcgctgcgc
     2641 tcaggatgac aacgtgggga tctcgatccg cgtgactaga agacatggat agaatccggg
     2701 tcgatgccga cctcgatgtg ctggccggcc gccggcagga cgccgcgggc aggctggcgg
     2761 accagcagct cgagtccgtc ggcgcaggcc aggcggagct tgaaggaggc gccgagatag
     2821 accgcctcca gcacctcgcc ggagaaagtg ttgacgccgc tcgcggcaaa tcgctcgggc
     2881 cgcatcagca cgcccaggcg atcgccgatg cgcgccgcgc cgttcaccag gagccgccgg
     2941 ccgcccgaca gcgtgatcac gccgggctcg gtcgaggtgc cgtggaagat gttgctctcg
     3001 cccacgaagt cggcgacgaa atcgttggcc ggccgctcgt agatctcggt ggtggtggcg
     3061 atctgctgga tggtgccctt gttcatcacc gcgacccggt cggacagcac cagtgcttct
     3121 tcctggtcgt gggtgatgaa gatcgtggtc aggccgagcc ggcgctgcag gcgccgcacc
     3181 tcgagctgca tggtctcgcg cagcttgcgg tcgagcgcac cgaacggctc gtcgagcagc
     3241 agcaggcgcg ggttgaagac gatggcacgg gcgagcgcca cgcgctgctg ctggccgccc
     3301 gagagctggc gcggcaggcg ggcctcgtag cccgcgagtt cgaccagcgc caaggcctcg
     3361 ccgacccggc gttcgatctc ggcgcgggcg acgttgcgca tctccagcgg gaaggcgacg
     3421 ttgcccttca ccgtgagatg gggaaacagc gcgtagttct ggaacaccat gccgatgtcg
     3481 cgccgcgccg gcggcagggc ggtgatctcg gcaccgtcga cccgcaccgt acccgcgtcg
     3541 ggcgtctcga agcccgccac gaccttgagc agggtggtct tgcccgatcc gctggggccg
     3601 aggatcgtca ggagctcgcc ttcggccacc gcgagcgaca cgccgtcgag cgccagaacc
     3661 tcgccgaagc gcttggtcac gccttcgact cgcagctccg ccgtacgcgc cctcgcctgc
     3721 tccgccactc cctacgcc
//
LOCUS       DASYJA010000373         3383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_418189, whole genome shotgun sequence.
ACCESSION   DASYJA010000373 DASYJA010000000
VERSION     DASYJA010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418189"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VGO02_08200"
     CDS             <1..662
                     /locus_tag="VGO02_08200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7477704.1"
                     /translation="ITQGELVLTLDGKETRHRAGDWYQVRAKAMHAARFEVETSEIEF
                     WFRAPAAGAVLIRKSVQADFAAMLAIVNDGAQAYRGVIPADRWHEPYMPADELAKEIA
                     AGIVFWVAEEAGRILGVMGMQDKGDVALVRHAYVATATQRSGVGTKLLRHVHGLTHQP
                     VLIGTWAAASWAVDFYKRNGFSVVSHADKEHLLRKYWSIPARQIETSVVLADARWMKE
                     G"
     gene            complement(665..1351)
                     /locus_tag="VGO02_08205"
     CDS             complement(665..1351)
                     /locus_tag="VGO02_08205"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV7477705.1"
                     /translation="MNFTVWRLALLGLGCAAVWAFIEIAEGVSEGGSREFDERLLLAL
                     RNRSDPADPIGPRWVEEMARDFTGLGGIGILGLITAASVVYLLMMRKRNAAVLVAAAV
                     GGGILLSALFKAGFDRPRPDLVSHLSQVYTTSFPSGHSMMSAVAYLTLGALLAQLHKG
                     RAVQAFILAVAILITVLVGLSRVYLGVHWPTDVLAGWAAGAAWACLCWLVAQWFQKRG
                     ALERGVEVSA"
     gene            1341..1919
                     /locus_tag="VGO02_08210"
     CDS             1341..1919
                     /locus_tag="VGO02_08210"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEV7477706.1"
                     /translation="MKFIAALWLALLASGAPAQSDRPNAVLLIAKPELIDPNFSRTVV
                     LVTQAESGETVGVILNRPSGRTHPRSADALFSGGPVMREVLLAVFRSTQPPEAPAFHV
                     LRGVYLTMHPANLDKLLSGEARDYRLYSGFSGWAPGQLESELQRDSWFVLPATENLLF
                     RADTNGMWEQLLEQVRGGRTDSRIIPQLALSK"
     gene            1967..2596
                     /locus_tag="VGO02_08215"
     CDS             1967..2596
                     /locus_tag="VGO02_08215"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEV7477707.1"
                     /translation="MTTALRWLVALAVCLLPGLAQAQQEDAVILVAHPAFRDLEYRQS
                     VLVAAPAPNGGHVGVIINRPTKRSLGSLFPEHEPSKKVVDPVYYGGPFSRGALVALVR
                     TDTAPGAGSVPLMKNLYLAFRANTIDHVIETTPNEARYFVGYVGWRPGELKSEIDRGL
                     WAVQDAELEMVFRKDTENLWEELQQASKRIRADSGASAATVPRLAAVGR"
     gene            2672..2950
                     /locus_tag="VGO02_08220"
     CDS             2672..2950
                     /locus_tag="VGO02_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477708.1"
                     /translation="MDAETGSRALHAANDLVEVLRLAQGAVERVAQEVHGAPFEEADL
                     VAQEMHRLRRLAERLQNDIERFVSNAESASVGRGHPLRRASDRHPSHP"
     gene            3202..>3383
                     /locus_tag="VGO02_08225"
     CDS             3202..>3383
                     /locus_tag="VGO02_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477709.1"
                     /translation="MPGGEMRFANEAQVEGTIDVQARVSRTDWVKNFAYPAPRTFTYS
                     EIVAADAGYVIGVDSNG"
BASE COUNT          570 a         1162 c         1115 g          536 t
ORIGIN      
        1 tcatcaccca gggcgaactt gtcctgaccc ttgatggcaa ggagactcgc caccgtgccg
       61 gcgattggta tcaggtccgc gcgaaagcaa tgcacgccgc gcgtttcgaa gtggaaacct
      121 cggagatcga gttctggttt cgcgcgcccg ccgcgggagc ggtgctgatc cgcaaaagcg
      181 tgcaggcgga tttcgcggcg atgctggcga tcgtcaacga cggcgcgcag gcctatcgcg
      241 gcgtgatacc ggcggaccgc tggcacgagc cgtacatgcc ggcggacgag ctggcgaagg
      301 aaatcgcggc gggcatcgtc ttctgggtgg cggaggaggc aggccgcatc ctgggcgtga
      361 tggggatgca ggacaaaggc gacgtcgctc tcgtgcgcca cgcgtacgtc gccaccgcca
      421 cgcaaagatc gggcgtgggg acgaagctgc tgcgccacgt gcacgggctg actcaccagc
      481 ccgtcttgat cggcacctgg gcggccgcgt cgtgggccgt cgatttctac aagcgcaacg
      541 gcttcagcgt ggtttcgcac gccgacaagg agcacctgct gcgcaagtac tggtcgatcc
      601 ccgcgcggca gatcgaaaca tcggtggtcc ttgccgatgc gcgctggatg aaggaaggtt
      661 agcgtcaggc agacacttcg acgccgcgct cgagcgcgcc gcgcttctgg aaccactgcg
      721 cgacgagcca gcacaggcaa gcccaggcgg cgcccgctgc ccacccggcg agaacgtcgg
      781 tcggccagtg cacgccgagg tagacccggc tcaggcccac caggacggtg atcaggattg
      841 cgacggccag aatgaaggcc tgcactgccc tgcccttgtg cagctgcgcg agcagggcgc
      901 cgagcgtgag gtaggccacg gccgacatca tcgagtggcc gctcggaaag ctcgtcgtgt
      961 acacctgcga gagatgcgac accaggtcgg ggcgcggccg gtcgaagccc gccttgaaca
     1021 gcgcgctgag gaggatcccg ccgccgactg ctgcggcgac caggactgcg gcgttacgct
     1081 tgcgcatcat cagcaagtag accacgcttg cggccgtgat cagcccgagg atgccgatgc
     1141 cgcccaaccc ggtgaagtcg cgggccatct cctccaccca gcgcgggccg atcgggtctg
     1201 ccggatcgct gcggttcctc agcgcgagca gcaagcgctc gtcgaactcg cgcgagccgc
     1261 cttcgctcac cccttcggca atttcgatga acgcccagac cgccgcgcag ccgagcccga
     1321 gcagcgccag cctccagacg gtgaaattca ttgccgcatt atggctcgcg ctgctcgcct
     1381 cgggcgcgcc agcgcagtcc gaccggccga acgcggtgct gctcatcgcc aagcccgagc
     1441 tgatcgaccc gaacttctcg cgcacggtgg tgctggtgac gcaagccgag tcgggcgaga
     1501 cggtgggtgt gatcctgaac cggccgagcg ggcgcacgca tccgcgcagc gcagacgcgc
     1561 tgttctccgg cgggccggtg atgcgcgagg tgctgctcgc tgtgttccgc tccacgcagc
     1621 cgcccgaagc tcccgcgttc cacgtgctgc gcggcgtgta cctcaccatg catccggcga
     1681 atctcgacaa gctcttgtca ggtgaggcgc gcgactaccg cctctactcc ggtttctctg
     1741 gttgggcgcc gggccagctc gagtccgagc tgcagcgcga ttcctggttt gtgctgcccg
     1801 ctaccgagaa cctgctgttc cgcgccgaca cgaacggcat gtgggagcag ctgctcgagc
     1861 aggtgcgcgg cgggcgcacc gattccagaa ttattccaca gctcgcgctg tcgaaatagt
     1921 tcaccccctt ttggcttggg cgaaaggcgt atacactttt actcagatga cgaccgcact
     1981 gcgttggctg gttgccctgg ccgtgtgtct ccttcccggc ctggcgcagg cgcagcagga
     2041 agacgccgtc atcctggtgg cgcacccggc gttccgcgac ctcgaatacc ggcagtccgt
     2101 tctggtcgcc gcgccggcac ccaacggcgg ccacgtcggc gtgatcatca accgccccac
     2161 caagcgctcg ctcggcagtc tgttcccgga gcatgagcct tcgaaaaagg tcgtcgaccc
     2221 ggtctattac ggcggtcctt tctcgcgcgg cgcgctggtc gcgctggtgc gcaccgacac
     2281 cgcgccgggg gcgggctcgg tgccgctcat gaagaacctg tatctcgcgt tccgtgccaa
     2341 caccatcgac catgtcatcg agaccacgcc gaacgaggcg cgctacttcg tcggctatgt
     2401 cggctggcgg ccgggagagc tgaagagcga gatcgaccgc ggcctgtggg cggtgcagga
     2461 cgccgagctc gagatggtgt tccgcaagga caccgagaac ctgtgggaag agctgcagca
     2521 ggcctcgaag cggattcgtg cagacagcgg cgcctcggcc gcgactgttc ccaggctcgc
     2581 cgccgtcggc cgctagcgac gcgccggccg gcgcaggccg cacccgcggc ggcaagccgg
     2641 aatttcttcc gccctggact atctttccag catggacgct gaaaccggca gccgcgcgct
     2701 gcacgccgcc aacgacctgg tggaagtcct gcgcctcgcg cagggtgccg tagagcgcgt
     2761 cgcccaggaa gtgcacggcg cgcctttcga agaagccgat ctggtcgccc aggaaatgca
     2821 ccgcctgcgc cgcctcgccg agcgcctgca aaacgacatc gagcgcttcg tcagcaacgc
     2881 ggaatccgcc tccgtcggcc gcggccaccc cctgcggcgc gcttccgacc gccatccctc
     2941 ccacccgtag ccgggaatag cccctgatcg gggctgtttg cagtacgctg cctatctcaa
     3001 cctctgggag gagggaccct gaagcgtctg ctcgcgttat tctcggtact ttccctgctg
     3061 tcctgcgcca ccggcccttc gaaggagcag cagctggtaa gcaaggcggt cgaggcgctg
     3121 ggcggcgccc aagccatcgc agacatccgc accatcacct acaagggcac ctggaagcaa
     3181 tgggagcccg agcaatccga cgtgcccggg ggcgaaatgc gcttcgccaa cgaggcgcag
     3241 gtcgagggca cgatcgacgt tcaggcgcgt gtttcgcgca ccgactgggt caagaacttc
     3301 gcctatcccg cgccgcgcac tttcacctac agcgagatcg tcgccgccga cgccggctac
     3361 gtcatcggcg tcgacagcaa cgg
//
LOCUS       DASYJA010000374         4212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1145058, whole genome shotgun sequence.
ACCESSION   DASYJA010000374 DASYJA010000000
VERSION     DASYJA010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145058"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VGO02_08230"
     CDS             complement(<1..513)
                     /locus_tag="VGO02_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV7477710.1"
                     /translation="MQVTWLFPGLFVVLWSTGFIGAKLGLPYAEPFTFLSLRFLCVLA
                     ILLPLCWLLKARWPNRQGIMHMAVAGVLMQGGYLGGVFASVHHGMPAGVSALITGLQP
                     VFTAVLGGWLLHERTTRRQWAGLALGLLGVVLVVGDRIAVQHLTVLAVLLSALALASI
                     TLGTVWQKRHG"
     gene            660..1202
                     /locus_tag="VGO02_08235"
     CDS             660..1202
                     /locus_tag="VGO02_08235"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEV7477711.1"
                     /translation="MYVTPEQIQAAGKANMEALLSVANAQFAAFEKLATINATAVKSA
                     FEDSIANTRALIGAKDVQEAVSLQGAFAQPAIEKAIAYSKSVYEVASEAGGELSKVAE
                     RRVAEWNENFVTLLDKVSKNAPAGSDVAVAAIKSMMAAGNAAYDNLNKVAKQATEIAE
                     ANVAAATETVKGLAKAKKAA"
     gene            complement(1304..2041)
                     /locus_tag="VGO02_08240"
     CDS             complement(1304..2041)
                     /locus_tag="VGO02_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEV7477712.1"
                     /translation="MFGAFIIGDELLVGKRQDKHLPFVIEALAKRGLRLSWAHYLGDE
                     PELIIEALRRSFASKDVVFSFGGIGATPDDHTRRCAAQALNVQLKLHPEAEREIRGRF
                     GSETTPQRLMMGEFPDGSSIIPNPVNRVPGFSLGEHHFVPGFPQMAWPMVEWVLDHRY
                     RSLFDRDRWSEASILVYEAGESQLIGAMEAVGAQYTGVKVFSLPSMGPKGERVHVELG
                     VRGDPAQVGGAIESLRSAVSKLGFPHK"
     gene            complement(2074..2811)
                     /locus_tag="VGO02_08245"
     CDS             complement(2074..2811)
                     /locus_tag="VGO02_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEV7477713.1"
                     /translation="MFTNREGQKVPQVTFRARTESDWKPVTTDDIFKGKTVVVFSLPG
                     AFTPTCSSTHVPRYNELAPAFLANGVERIVCLSVNDAFVMNEWARTQECGNVTLLPDG
                     NGEFTEKMGMLVDKSELGFGKRSWRYSMLVKDGVIQKMFIEPEKQGDPFEVSDADTML
                     DFINPKARKPDQVAILTRDGCGYCAKAKALLKELGYAYSEVKLDHSDRSRVVGAITGR
                     GTVPQVFVNGQHIGGLEDLQRWSKKAA"
     gene            complement(2854..3624)
                     /locus_tag="VGO02_08250"
     CDS             complement(2854..3624)
                     /locus_tag="VGO02_08250"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HEV7477714.1"
                     /translation="MSVARSMLYGFINLLHPRMLWLMVWPMLVSLAVWGTVAIVLWVR
                     TAVWLGGVLRQWLESAPYLGQLNLADATLIAAHVLLFLLFVPLVYLTALFILGIFGMQ
                     AMVEHVARRSYPQLELRRGGSAFGSVWNGLVALAGMALLALVSFPLWLAPPLWPVLAV
                     AILGWGNQRLLRYDALAEHADRGEMQRVFRGHRLRLYVMGALLALIAYVPLLGLLAPV
                     AFGLAFIHYLLGALAAERIGPAYRGHPNKELDGGNAAP"
     gene            complement(3640..4068)
                     /locus_tag="VGO02_08255"
     CDS             complement(3640..4068)
                     /locus_tag="VGO02_08255"
                     /inference="COORDINATES: protein motif:HMM:NF023326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEV7477715.1"
                     /translation="MASLFGDIGTGVFNSCAKNFPRESEDVKRQLERLLGPGWLARTS
                     WPRLQHFARYLKAASLRLDKLRADPARDARLAAEIASLQAAWLREVAAQARYGAPSAE
                     LEQFGWLLEELRVSLFAQELKTPVPVSPKRLAKLWQTMRK"
BASE COUNT          835 a         1379 c         1336 g          662 t
ORIGIN      
        1 gccgtgccgc ttctgccaga ccgtgcccag cgtgatgctg gcgagcgcca gggcggaaag
       61 aaggaccgcg agcacagtca ggtgctgcac ggcgatccgg tcgcctacga ccagaacgac
      121 gcctagcagc ccgagcgcca ggccggccca ttgccgccgg gtggtccgtt catggagtag
      181 ccagccgccg aggactgccg tgaacaccgg ctgcaggcct gtgatcaggg ccgacacccc
      241 cgccggcatg ccgtgatgca ccgaagcaaa aacacccccc agataccccc cttgcatgag
      301 cacccccgcc acagccatat gcatgatgcc ctggcggttc ggccagcgag ccttcaagag
      361 ccagcagagg ggcagcagga tggcaaggac gcagaggaag cgaagagaaa ggaaagtgaa
      421 gggttccgcg taaggaagcc ctaacttcgc cccgatgaac ccggtgctcc agaggaccac
      481 gaacagcccc ggaaatagcc acgtaacctg catcgggcac gattgtagtg ccggagacga
      541 tagggcaatc acgattgctg ctacgcacaa atttcttgac ttcctacacg atcagcctag
      601 aatctaactt tgctgcattg cacaaaacgc actaacccct aattcaggag ctgacgaaaa
      661 tgtacgtaac cccggaacaa attcaagcgg ccggcaaggc caacatggaa gcgctgcttt
      721 ccgtggcgaa cgcgcaattc gcggcgttcg aaaagctggc gaccatcaac gccacggcag
      781 tcaagagcgc cttcgaggac tcgattgcca atacccgcgc cctgatcggc gcgaaagacg
      841 tgcaggaagc cgtaagcctg cagggcgcct ttgcccagcc cgccatcgag aaggccatcg
      901 cctactcgaa gagcgtgtac gaagtcgcca gcgaagccgg cggcgagctg tcgaaagtgg
      961 ccgagcgccg cgtcgcggag tggaacgaga acttcgtcac cctgctcgac aaggtgtcga
     1021 agaacgcgcc ggccggctcg gatgtcgcgg tcgccgcgat caagtcgatg atggccgcgg
     1081 gcaatgccgc gtatgacaac ctcaacaagg tcgcgaagca ggcgaccgag atcgccgaag
     1141 ccaatgtcgc cgccgccacc gagacggtga aggggctggc caaggcaaaa aaggccgcct
     1201 aagcgccaaa ccgcgcggga agcagaagtc acccgcccga tctagcgcga agaagaaagc
     1261 cgcaaagaaa gctgtaaaga aagcccgccg ctaggcgggc tttttatttg tgcgggaatc
     1321 ccagcttgct cacggccgag cgcagggatt cgatcgctcc gcccacttgg gcgggatcgc
     1381 cccgcacgcc cagctcgacg tggacccgct cgcccttcgg gcccatggag ggcaggctga
     1441 agactttcac gcccgtgtac tgcgcgccca ccgcctccat ggcgccgatg agctggcttt
     1501 cgcccgcctc gtagaccagg atcgaggcct cgctccagcg atcgcggtcg aacaggctcc
     1561 gatagcggtg gtcgagcacc cactccacca tcggccaggc catctgcgga aagcccggca
     1621 cgaagtggtg ctcgccgagc gagaacccgg gaacgcggtt gaccggattg ggaatgatgc
     1681 tcgagccatc gggaaactcg cccatcatca gccgctgcgg cgtcgtctcg ctgccgaagc
     1741 gcccgcgaat ttcccgctcc gcctcggggt ggagtttcaa ttggacattc aaggcttggg
     1801 cggcacatcg gcgcgtgtgg tcgtcaggcg tggcgccgat accgccaaag ctgaacacca
     1861 cgtcctttga ggcgaagctg cgccgcaacg cctcgatgat cagctccggc tcgtcgccga
     1921 gatagtgcgc ccaggaaagg cgaagccctc gcttggcgag ggcttcgatg acgaacggca
     1981 ggtgcttgtc ctggcgcttt cctacgagaa gctcatcccc gatgatgaaa gcgccgaaca
     2041 aatttcaggc tgctttctgc tcttcgagcg gcttcacgcg gccttctttg accaccgttg
     2101 caggtcttca aggccgccga tgtgctggcc gttgacgaag acctgcggca ccgtgccgcg
     2161 gccggtaatg gcgccgacga cgcgcgagcg gtcgctgtgg tcgagtttca cttccgagta
     2221 agcatagccc agctctttca gcagcgcttt cgctttcgcg cagtagccgc agccgtcgcg
     2281 ggtcaggatc gccacctggt cgggcttcct cgccttcggg ttgatgaagt cgagcatggt
     2341 gtcggcgtcc gacacctcga acgggtcgcc ctgcttctcc ggctcgatga acatcttctg
     2401 gatcacgccg tccttcacca gcatcgagta gcgccacgag cgcttgccga agccgagctc
     2461 cgacttgtcg accagcatgc ccatcttctc ggtgaattcg ccgttgccgt cgggcagcag
     2521 cgtcacgttg ccgcactcct gggtgcgcgc ccattcgttc atgacgaaag catcattcac
     2581 ggagaggcac acgatgcgct cgacgccgtt ggcgaggaac gccggcgcca gctcgttgta
     2641 gcgcggcacg tgagtggacg agcacgtcgg ggtgaacgcg ccgggcagcg agaacacgac
     2701 cacggtcttg cccttgaaga tgtcgtccgt cgtcaccggc ttccagtcgc tctcggtgcg
     2761 cgcgcggaac gtaacctgcg gaactttctg gccttcgcga ttcgtaaaca cgtctgctgc
     2821 tcctgtcctg aatggtgagg aatggacagc agtctaggga gcggcgttcc cgccatccaa
     2881 ttctttgttc ggatggcccc gataggcagg gccaatccgc tccgccgcca gcgcgcccag
     2941 caggtagtgg atgaaggcca ggccgaaggc gaccggcgcg agcaggccca gcagcggcac
     3001 gtacgcgatc agcgcgagga gcgcacccat gacgtacagg cgcaggcggt ggccgcgaaa
     3061 cacgcgctgc atctcgccgc ggtcggcgtg ctcggcgagc gcgtcgtagc gcagcagccg
     3121 ctggttgccc cagcccagga tggccaccgc gaggaccggc cacagcggcg gcgcgagcca
     3181 tagcggaaag ctgacgaggg cgagcagcgc cattccggcc agcgcgacca acccgttcca
     3241 gacgctgccg aatgcgctgc cgcctcggcg cagctcgagc tgcggatagg agcggcgcgc
     3301 gacgtgctcg accatcgcct gcatgccgaa gatgccgagg atgaacagcg ccgtcaggta
     3361 gaccagcggc acgaacagca ggaacagcag cacgtgcgcc gcgatcaggg tggcgtctgc
     3421 caggttgagc tgcccgaggt atggcgccga ctccagccat tgcctgagga cgccgccgag
     3481 ccagaccgcg gtgcgcaccc acagcacgat ggccacggtg ccccatacgg cgagcgacac
     3541 cagcatcggc cacaccatca accacagcat gcgcgggtgc agcaggttga tgaagccgta
     3601 cagcatggag cgggcgacgc tcatagagtc attttcgtct catttcctca tggtctgcca
     3661 gagcttggcg agtctttttg gcgataccgg gaccggcgtc ttcaattcct gcgcgaagag
     3721 cgacacgcgc agctcctcga gcagccagcc gaactgctcg agctcggcgg agggtgcgcc
     3781 gtagcgcgcc tgcgctgcta cttcgcgcag ccaggccgcc tgcagcgacg cgatctcggc
     3841 ggcgaggcgc gcgtcgcgcg ccggatcggc gcgcagcttg tccagacgca gcgacgccgc
     3901 cttgagatag cgcgcaaaat gctgcagccg cggccacgac gtgcgcgcga gccagccggg
     3961 cccgagcagc cgctcgagct ggcgcttcac atcctcgctc tcccgcggaa agttcttcgc
     4021 gcaggaattg aagacgccgg tcccgatatc gccaaaaaga ctcgccaagc tctggcagac
     4081 catgaggaaa tgagacgaaa atgattctat gagcgtcgcc cgctccatgc tgtacggctt
     4141 catcaacctg ctgcacccgc gcatgctgtg gttgatggtg tggccgatgc tggtgtcgct
     4201 cgccgtatgg gg
//
LOCUS       DASYJA010000375         2868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1962382, whole genome shotgun sequence.
ACCESSION   DASYJA010000375 DASYJA010000000
VERSION     DASYJA010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1962382"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..116
                     /locus_tag="VGO02_08260"
     CDS             <1..116
                     /locus_tag="VGO02_08260"
                     /inference="COORDINATES: protein motif:HMM:NF015691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HEV7477716.1"
                     /translation="PVSADTLCLHGDQPGAVAFAKEIRKVFSERGISLGAP"
     gene            103..1218
                     /locus_tag="VGO02_08265"
     CDS             103..1218
                     /locus_tag="VGO02_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEV7477717.1"
                     /translation="MAHPKLFTPLPLGGIVLPNRIAVSPMCQYSADDGCMTDWHLIHL
                     GQLAYSGAGLLVVEATGVTREGRITHGCTGLYTDDNEAAMARVIQACRRLSQNPIGVQ
                     LAHAGRKASVHVTWKGGKPLGPGESPWETVAPSPIPFGEGWHVPHEMEVAEIKKLVEN
                     FVASALRAKRIGFDAVELHSAHGYLLHQFLSPLSNQRSDSYGKNRMQFPLEVAQAVRD
                     VWPKERALGARISGSDWMEGGLGPDDAVAYAKELKRIGYDYVCVSSGALSPLARMKVE
                     PNYQVPFAERVKKNVDINVRAVGMIADPEQAEDIVASGKADMIAMARAFLDNPRWVWH
                     AAERFGIKLDYPPQYSRSRPDVWPGSKLARAPSEQRS"
     gene            1222..1740
                     /locus_tag="VGO02_08270"
     CDS             1222..1740
                     /locus_tag="VGO02_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
                     /protein_id="HEV7477718.1"
                     /translation="MTIEGFLAAARQALAGASPQQAMRSLLDATVEALHARTSRWIEH
                     DEDEMLLASSAQLTVYHIALSPRIHYPPHDHRVPAMIALYQGAETSFSYRRKGRALVQ
                     SKRHDHAAPCVASLPADAIHSVVNLGDVRSAAIHVYFGDLTAVERSIWDADLREERRF
                     DNRFYFEQARKL"
     gene            complement(1762..2217)
                     /locus_tag="VGO02_08275"
     CDS             complement(1762..2217)
                     /locus_tag="VGO02_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEV7477719.1"
                     /translation="MVDILHRVGIKASVDQVYKALTTRAGLAGWWTTDTQGDSKVGGI
                     LKFRFTRDGKELGGFDMKVLELQPGRRVLWQVVEGPAEWVGTKIGFELRQEGDYSIVL
                     FKHQGWKEPAEFMHHCSTKWAIFLMSLKSLVETGKGQPTPDDVQISDWH"
     gene            2322..>2868
                     /locus_tag="VGO02_08280"
     CDS             2322..>2868
                     /locus_tag="VGO02_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004544296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7477720.1"
                     /translation="MDSLITAAGRALAAGDPLGALKLVALREDAPALALRGIAMAQLG
                     DLARAKALLRSAARAFSPKEAVSRARCIVAKAEVALASRDLGWDAKALDAARATLEAH
                     GDRVNAAHARYLEVRRLPLIGRLDEAERKLELLDPAPLPPALRAAHELGVAGIAMRRL
                     RTKAARAALARAEHAARHARIP"
BASE COUNT          488 a          979 c          916 g          485 t
ORIGIN      
        1 tgccggtttc ggccgacacg ctgtgcctgc acggcgacca gcccggcgcc gtggcgttcg
       61 cgaaggaaat cagaaaagta ttttccgaaa gaggaatttc tcttggcgca ccctaagctc
      121 tttacgccgc taccgctcgg cggcatcgtg ctgcccaacc gcatcgcggt ctcgccgatg
      181 tgccagtact cggccgacga cggctgcatg accgactggc acctgatcca tctcggccag
      241 ctcgcctatt ccggcgccgg cctgctggtg gtcgaagcca ccggcgtgac gcgcgagggc
      301 cgcatcacgc acggctgcac cggcctctac accgacgaca acgaagcggc gatggcgcgc
      361 gtcatccagg cctgccggcg cctgtcgcag aatcccatcg gggtccagct tgcccatgcc
      421 ggccgcaagg cctcggtcca cgtgacctgg aagggtggca agccgctggg cccgggcgag
      481 tccccctggg agaccgtcgc gccttcgccg attcccttcg gcgaggggtg gcatgtcccg
      541 catgaaatgg aagttgctga gatcaagaag ctggtggaga acttcgtcgc gtcggcgctg
      601 cgcgccaagc gcatcggctt cgacgcggtc gagctgcatt cggcgcacgg ctacctgctg
      661 catcagtttc tgtctccgct ttccaaccag agaagcgatt cctacgggaa aaacaggatg
      721 caattccccc tggaagtcgc gcaagcagtg cgcgacgtct ggccgaaaga gcgcgctctc
      781 ggcgcgcgca tcagcggcag cgactggatg gaggggggcc tcgggcccga cgacgcagtc
      841 gcctatgcga aggaactgaa gcgcatcggc tacgactacg tctgcgtctc gagcggcgcc
      901 ctgtcgccgc tggcgcgtat gaaggtggaa cccaactacc aggttccgtt tgcagaacgc
      961 gtaaagaaaa acgtagacat taacgtccgc gccgtcggca tgatcgccga cccggagcag
     1021 gccgaggaca tcgtcgcctc cggcaaggcc gacatgatcg ccatggcacg cgccttcctc
     1081 gacaacccgc gctgggtctg gcacgccgcc gagcgcttcg gcatcaagct cgactacccg
     1141 ccgcaatact cccgcagccg cccggacgtc tggcccggtt cgaagctcgc cagggcacct
     1201 tcagaacagc gctcctagga aatgaccata gagggctttc ttgccgccgc ccgccaagcg
     1261 ctggccggcg cttcgccgca gcaggcaatg cgctcgctgc tcgatgccac ggtcgaggcg
     1321 ctgcacgccc gaaccagccg atggatcgag cacgacgagg acgaaatgct gctggcttcc
     1381 tctgcgcagc tcaccgtcta tcacatcgcg ctgtcgccgc gtatccacta tccgccgcac
     1441 gaccatcgcg tgcccgcgat gatcgccctc taccaggggg cggagacgag cttctcgtac
     1501 cggcgcaaag gcagggcgct ggtgcagtcg aagaggcacg accacgcggc accctgcgta
     1561 gcgtctctgc cggccgacgc aatccattcc gtagtcaacc tgggcgatgt gcgttccgct
     1621 gccatccacg tctatttcgg cgatttgacc gcggtagagc gcagcatctg ggacgccgat
     1681 ctgcgcgagg agcgacggtt cgacaaccgc ttctacttcg agcaggcgcg aaagctgtga
     1741 ccggagaacc agagccgctc ctcagtgcca gtcgctgatc tgcacgtcgt cgggggtggg
     1801 ctggcctttc ccggtctcca ccagcgactt caggctcatc agaaaaatcg cccacttggt
     1861 gctgcagtgg tgcatgaact ccgccggctc cttccagccc tggtgcttga acaggacgat
     1921 ggaatagtcg ccttcctgtc tgagctcgaa accgatcttt gttccgaccc attcggcggg
     1981 cccttcgacc acttgccaca gcacgcgcct gccgggctgg agctcgagga ccttcatgtc
     2041 gaagccgccg agctctttgc cgtcccgggt gaagcggaac ttgaggatgc cgccgacctt
     2101 gctgtcgcct tgcgtgtcgg ttgtccacca gccggccagg cctgcgcgcg tggtcagcgc
     2161 cttgtagacc tgatcaacgg acgccttgat gccgactctg tgaagaatat cgaccatgat
     2221 gcttctcctt tcgggttgat tgaaaacgac gctgtcgtcg tggccgagag tctcggcgcg
     2281 aagccccgaa gggtgggagt gacaagtgtg tcgggatttg catggactca ttgatcacgg
     2341 ccgcagggcg ggcactggcg gcgggcgatc cccttggcgc cttgaagctg gtcgcgctgc
     2401 gcgaagacgc gccggcgctc gcgctgcgcg gcatcgccat ggcgcaactg ggtgatctcg
     2461 ctcgcgcaaa ggcgctgctg cgaagcgccg cgcgcgcctt cagtcccaaa gaagccgtgt
     2521 cccgcgcgcg ctgcatcgtc gccaaagccg aggtcgcgct cgcctcgcgc gacttgggct
     2581 gggatgcaaa ggcgctcgac gccgcgcgcg caacgctcga agcccacggc gatcgcgtga
     2641 acgccgcgca tgcgcgctat ctggaagtcc ggcgcctccc gctgatcggt cgcctcgacg
     2701 aagccgagcg caagctcgag ctgctggacc ccgcgcccct gccgcccgcg ttgagggcgg
     2761 ctcatgaact gggggtggcg ggcatcgcga tgcgccgctt gcgcacgaag gcggcgcgcg
     2821 cggcgcttgc ccgcgccgag cacgcagcgc gccacgcacg cattcccg
//
LOCUS       DASYJA010000376         5643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1417092, whole genome shotgun sequence.
ACCESSION   DASYJA010000376 DASYJA010000000
VERSION     DASYJA010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417092"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VGO02_08285"
     CDS             complement(<1..324)
                     /locus_tag="VGO02_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477721.1"
                     /translation="MGGTRAVFLSLLVGLLPAPALAASSPSRIPDAPVFDSSTLSRIA
                     GWLKRDGREGWLGADVADAVGIPRLATEDLLEARQRGFRDDEALRIVQVSADERRDFL
                     LFMVQH"
     gene            complement(360..569)
                     /locus_tag="VGO02_08290"
     CDS             complement(360..569)
                     /locus_tag="VGO02_08290"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HEV7477722.1"
                     /translation="MERKRVNSSKLRSVGYDEPTRTLEVEMSNGQVFQYPGVYPEVYR
                     RFMAAPNPTSFFDDKIAEEYTARKV"
     gene            complement(571..1389)
                     /locus_tag="VGO02_08295"
     CDS             complement(571..1389)
                     /locus_tag="VGO02_08295"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV7477723.1"
                     /translation="MAFAVSLNFSFPAGAEDVKPVKDAGPYVPSPQSVVSDMLRHAEV
                     GPADFLIDLGSGDGRIVLTAAKVFGARGFGVEIKDDLVTKSNQAAQNEGLADRVKFIK
                     QDLFKTDLSQATVVTMYLLPDTVNMLKDKFLAELKPGTRIVSHDYPLTGWIPEKYVQM
                     DLEDKVQISGVTTTLIYLYVVPAKVAGNWSAKMPPAVSKAPATLSLRQQLTRVSGSLR
                     IDGKDVPLEDVKLRGDRLTFKLAGRKGEYAGQIKGKAIEGTFDGRTPWSATLGS"
     gene            complement(1398..3089)
                     /gene="recJ"
                     /locus_tag="VGO02_08300"
     CDS             complement(1398..3089)
                     /gene="recJ"
                     /locus_tag="VGO02_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEV7477724.1"
                     /translation="MKIIERSWSARDRDQLVAGGVHPVLARVYAGRSIRNSKDLEYDP
                     ALLHSPALLKGIDDAARLLADAIAAKKRLLIVADYDADGATACAVGLRALRAMMGNSG
                     ATVDYLVPDRFKLGYGLSPELVDLAAQKTPDLLITVDNGIASVEGVARAAALGIATLI
                     TDHHLPGDALPAANCIVNPNQLGCGFPSKALAGVGVMFYVMLALRSELRKRDFFKEQK
                     EPNLGALTDLVALGTIADVVPLDANNRNLVSQGLKRLRAGRGQHGIVALLRAAGRNPA
                     EASSFDLGFIAGPRLNAAGRLADMSLGIECLVADDEARAANLAQQLDRFNRERKKIES
                     DMLDGALSLLENLSSEEQGSFFEPTWHEGVVGILASRLKDKLHRPVVCFARAADGGLL
                     KGSGRSIAGLHLRDCLDLVAKRHPGLILRFGGHAMAAGLTIAESGYARFAAAFERSLD
                     EMLPESARLRVVETDGALEPAHRTLEVARLIETQIWGQGFPQPLFCDTFAVESQRVVG
                     DKHLKLMLAKDGRRIEAMRFGALQPLPAQVRAAYRLGVNDFNGLKTVQLNVEHFE"
     gene            complement(3086..4075)
                     /locus_tag="VGO02_08305"
     CDS             complement(3086..4075)
                     /locus_tag="VGO02_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477725.1"
                     /translation="MHCELIVPSLFATATEARYPALELLLARGRRESAESRTLEQWLR
                     QAFEQPGEALAAGALSLAAHGGSPGERWWARADPVHLRVMRDRAIVAPGEALEVASDE
                     ADALVEALNRHFAGAVEFRVLEPRRWVARLEPGTGVTEGPALYAAGRDAAIARGEEGL
                     RAALLTEIQMLLHAHPVNEAREARGEPALNSLWIWGGGQPPRAAHGPWRSVAAADPSA
                     LGLAQLAGMRGLDLPPSAAAWLDLLPVDGRHLAVLDVLRVPTALEQQAEYGVGIEALE
                     RLWFAPLLAALRAERIGMVTLRVPDAAPGAGFETVRGDLRRFWRRPRALGRYA"
     gene            complement(4060..5463)
                     /locus_tag="VGO02_08310"
     CDS             complement(4060..5463)
                     /locus_tag="VGO02_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEV7477726.1"
                     /translation="MRLLDAPLAIVDLETTGTDPAGDRITEIAVLEVQGFALTSEWST
                     LVNPGAGTPSPIQALTGITNEMVARAPSFAQVAEELHELLRGRVFVAHNARFDYGFLR
                     REFDRAGIRFTAKTLCTVRLSRRLYPREPHHNLDSLIARHGIDCRQRHRAMGDADALW
                     QFLQIAEREHGEEVVAVAARQIARQPTLPPQLERAAVDAVPEAPGVYLFYDEASVPLY
                     IGKSASMRTRVMAHFCGASSQRSVELSKRVRRIDWKRTAGELGALLLEARLVKELAPE
                     FNRQLRATEGLCGFAFDGRRLRFAGAHEIDGETLPFVYGVFRTKRAALQALRGLADEH
                     RLCLQALGFETGPQHACFRHQLGRCAGVCAGRESVHLHHARLAAALASWKAADWPHSG
                     PLGIVERGRDGEEAEMHVVDRWCYLGAARSDAEVAELLEGGRRGRFDFDHYRILARHL
                     GKRGVRTVRLAAPCTAS"
BASE COUNT          854 a         1923 c         1994 g          872 t
ORIGIN      
        1 atgctgcacc atgaacagca ggaagtcgcg ccgctcgtcg gccgagacct gcacgatgcg
       61 cagcgcctcg tcgtcgcgga agccgcgctg gcgcgcctcg agcaggtctt cggtggcgag
      121 gcgagggatg ccgaccgcgt cggcaacgtc ggcgccgagc cagccctcgc ggccgtcgcg
      181 cttcagccag ccggcgatcc ggctgagggt gctgctgtcg aagacgggtg catccgggat
      241 gcggcttgga gaagacgcgg cgagcgccgg cgccggcaaa agaccaacca gcaggctgag
      301 aaagactgcg cgagtgcccc ccataggccc ttccctttct tgccttcagt ctacgccgat
      361 cagactttcc tcgcggtgta ctcctccgcg atcttgtcgt cgaagaacga ggtggggttg
      421 ggcgccgcca tgaagcgccg gtagacctcg ggatagacgc ccgggtactg gaacacctgc
      481 ccgttcgaca tctcgacttc cagcgtgcgg gtcggctcgt cgtagccgac cgagcgcagc
      541 ttggaagaat ttacacgctt acgttccacg tcagctacct agagtcgcgc tccacggcgt
      601 cctgccgtcg aaagtgccct cgatcgcctt gcccttgatc tggccggcgt actcgccctt
      661 gcggccggcg agcttgaagg tcaggcggtc gccgcgcagc ttcacgtcct cgagcgggac
      721 gtccttgccg tcgatgcgca ggctgcccga gacgcgcgtc agctgctggc gcagcgagag
      781 cgtcgccggc gccttgctca ccgcgggcgg catcttagcg ctccagttgc cggcgacctt
      841 cgccggtacg acgtagagat agatcagcgt ggtggtgacg ccgctgatct gcaccttgtc
      901 ctcgaggtcc atctgcacgt acttctcggg gatccagccg gtgagcgggt agtcgtgcga
      961 cacgatgcgc gtgccgggct tgagctcggc gaggaacttg tccttcagca tgttcacggt
     1021 atcgggcagc aggtacatcg tcaccaccgt ggcctgcgac agatcggtct tgaacagatc
     1081 ctgcttgatg aacttgacgc ggtcggcgag gccttcgttc tgcgctgcct ggttcgactt
     1141 ggtgaccaga tcgtccttga tctcgacgcc gaagccgcgc gcgccgaaca ccttggccgc
     1201 ggtgagcacg atgcgcccgt cgcccgagcc caggtcgatg agaaagtcgg cgggcccgac
     1261 ctcggcgtgg cgcagcatgt cggagaccac gctctgcggc gagggcacgt aggggccggc
     1321 gtccttcacc ggctttacgt cttcagcgcc ggccgggaag gagaaattca gggaaactgc
     1381 aaatgccaag gcgcggatca ttcgaagtgc tccacgttga gctggacggt cttcagtccg
     1441 ttgaagtcgt tgacgcccag gcggtaggcg gcgcgcacct gcgcgggcag cggctgcagg
     1501 gcgccgaagc gcatcgcctc gatcctgcgg ccatctttcg ccagcatcag cttgaggtgc
     1561 ttgtcgccga ccaccctctg gctttcgacg gcgaatgtat cgcagaagag cggctgcgga
     1621 aagccctggc cccagatctg cgtctcgatg aggcgcgcca cctcgagcgt gcggtgcgcc
     1681 ggctcgaggg cgccgtcggt ctcgaccacg cgcagccgcg cgctttccgg cagcatttcg
     1741 tcgagcgagc gctcgaacgc cgcggcgaag cgcgcgtagc cgctctcggc aatggtcagg
     1801 ccggcggcca tggcgtgccc gccgaagcgc aggatcaggc cgggatggcg cttggcgact
     1861 aggtcgaggc agtcgcgcag gtgcagcccc gcgatcgagc gccccgagcc cttcagcagg
     1921 ccgccgtccg ccgcgcgcgc gaagcagacc acggggcggt gcaatttgtc cttgagccgc
     1981 gacgcaagga tgcccaccac gccttcgtgc catgtgggct cgaagaagct gccttgctct
     2041 tcactcgaaa gattttcaag gagagaaaga gccccatcca gcatgtccga ctcgatcttc
     2101 ttgcgctccc ggttgaagcg atcgagctgc tgcgcgaggt tggcggcgcg cgcctcgtcg
     2161 tcggcgacca ggcactcgat gcccaggctc atgtcggcga ggcgccccgc ggcgttgagc
     2221 cgcggcccgg cgatgaagcc caggtcgaag ctcgaggcct cggcggggtt gcgcccggcg
     2281 gcgcgcagca gcgcgacgat gccgtgctgg ccgcggccgg cgcgcaggcg cttgaggccc
     2341 tgcgagacga ggttgcggtt gttggcgtcg agcggcacca cgtcggcgat cgtgccgagc
     2401 gcaacgaggt cggttagcgc tccaagattt ggctcttttt gctctttgaa gaaatctctc
     2461 tttcttaatt cacttcttaa agcgagcatg acgtagaaca tgacgcccac tcccgccagg
     2521 gccttggagg gaaaaccaca gcccaactgg ttcgggttga cgatgcagtt cgcggcgggc
     2581 agcgcatcgc ccggcaggtg gtggtcggtg atcagcgtcg cgatacccag cgccgcggcg
     2641 cgcgccacgc cctcgacgct ggcgatgccg ttgtcgacag tgatcagcag gtccggggtt
     2701 ttctgcgcgg cgagatcgac cagctcgggc gacaggccgt agccgagctt gaagcggtcg
     2761 gggaccaggt agtcgacggt agcgcccgaa tttcccatca tggcgcgcag cgcgcgcagt
     2821 ccgacggcgc aggcggtcgc tccgtcggcg tcgtagtcgg cgacgatcag cagccgcttt
     2881 tttgcggcga tggcgtcggc aagaaggcgc gcagcgtcgt cgatgccttt gagcaacgcg
     2941 ggtgaatgga ggagagcggg gtcgtactcg aggtcttttg aattccggat cgagcgacct
     3001 gcatagaccc gtgccagcac cgggtgaacg ccgcccgcga ccaactggtc ccggtccctg
     3061 gccgaccagc tgcgctcgat gatcttcatg cgtaccgccc aagcgcgcgc gggcggcgcc
     3121 agaagcggcg cagatctccg cgcaccgtct cgaagccggc gcccggcgcg gcgtcgggca
     3181 cgcgcaaggt gaccatgccg atgcgctcgg cgcgcaacgc cgcgagcagc ggggcgaacc
     3241 agaggcgctc cagcgcctcg atgcccaccc cgtactccgc ctgctgctcg agggcggtcg
     3301 gcacgcgcag cacgtcgagc accgcgaggt ggcggccatc cacgggcaac agatcgagcc
     3361 aagccgcggc ggagggcggc agatcgaggc cgcgcatgcc ggcgagctgc gccaggccga
     3421 gcgccgatgg atcggcggcg gccaccgagc gccacgggcc gtgcgccgcg cgcggcggct
     3481 ggccgccacc ccagatccac aggctgttca gcgccggctc gccgcgcgcc tcgcgcgctt
     3541 cgttgaccgg gtgcgcgtgc agcagcatct ggatttctgt aagaagcgcg gctcgcaggc
     3601 cctcttcgcc ccgcgcgatc gcggcatcgc ggcccgccgc atacagcgcc gggccttcgg
     3661 taacgcccgt gccgggttcc aggcgcgcga cccagcggcg aggctcgagc acgcgaaatt
     3721 ccaccgcgcc ggcgaagtgc cggttgagcg cctcgaccag cgcgtcggct tcgtcggagg
     3781 cgacctccag tgcctcgccc ggcgccacga tcgcgcgatc gcgcatgacc cgcaggtgca
     3841 ccgggtcggc ccgcgcccac caccgctcgc ccgggcttcc gccgtgcgcg gcgagcgaga
     3901 gcgcgccggc ggcgagcgcc tcgccgggct gctcgaatgc ctggcgcagc cattgctcga
     3961 gggtgcggga ttccgcgctc tcgcgccggc cgcgcgcgag cagaagctcg agggcggggt
     4021 agcgcgcctc ggtcgccgtg gcgaacaggc tcgggacgat cagctcgcag tgcacggcgc
     4081 ggcgaggcgg acggtgcgca cgccgcgctt gcccagatgg cgcgcgagga tgcggtagtg
     4141 gtcgaaatcg aagcgtccgc gccgcccgcc ctcgagcagc tcggccacct cggcgtcgga
     4201 gcgcgccgcg ccgaggtagc accagcggtc gacgacgtgc atttcggctt cttcgccgtc
     4261 gcgaccgcgc tcgacaatac ccagcggtcc cgaatgcggc cagtcggcgg ccttccacga
     4321 ggcgagcgcc gcagcgaggc gcgcatgatg caggtgcacg ctttcgcgcc ctgcgcagac
     4381 gccggcgcag cggccgagct ggtgccggaa gcaggcgtgc tgcggccccg tttcaaagcc
     4441 gagggcctgc aggcagaggc ggtgctcgtc cgccaggccg cgcagcgcct gcagcgcggc
     4501 gcgcttcgtg cggaacacgc cgtaaacgaa aggcagcgtc tcgccgtcga tctcgtgcgc
     4561 gccggcgaag cgcaggcggc ggccgtcgaa ggcaaagccg cacaggcctt cggttgcgcg
     4621 cagctggcgg ttgaactccg gcgccagctc cttgaccagc cgcgcctcga ggagcagggc
     4681 gccgagctcg ccggcggtgc gcttccagtc gatgcgccgt actcttttcg atagttcaac
     4741 agatcgctgg gagctcgcgc cgcagaaatg cgccatgacg cgcgtacgca tggaagcgct
     4801 tttgccgatg tatagaggga cgcttgcctc gtcgtagaaa aggtagacgc ccggtgcctc
     4861 gggaaccgcg tcgaccgcgg cgcgctcgag ctgcggcggc agcgtcggct ggcgcgcgat
     4921 ctgccgcgcg gcgacggcga cgacttcctc gccgtgctcg cgctcggcga tctgcaggaa
     4981 ctgccacagc gcgtcggcgt cgcccatcgc gcgatggcgc tggcggcagt cgatgccgtg
     5041 gcgcgcgatc aggctgtcga ggttgtggtg cggctcccgc ggatagagcc ggcgcgacag
     5101 gcgcaccgtg cacagcgtct tggccgtaaa gcggatgccg gcacggtcga actcgcggcg
     5161 caggaagccg tagtcgaagc gcgcgttgtg cgcgacgaag acccttccac ggaggagctc
     5221 gtggagctcc tccgcaacct gggcgaagct cggggcgcga gcgaccatct cgttggtgat
     5281 gccggtcagc gcctggatcg ggctgggggt gccggcgccg gggttgacca gcgtcgacca
     5341 ttcggaggtg agcgcgaagc cctgcacctc gagcaccgcg atttcggtta tgcggtcgcc
     5401 ggccgggtcg gtgccggtgg tctccaggtc gacgatcgcc agcggcgcgt ccagcaggcg
     5461 catctcactg cgccttgacg ttcgccgtct tgatcacctc accccacttc gccgcgtcgg
     5521 agcggatgcg ctcgacgaac tgctcgggcg tgctgccgac cggctccgcg ccgaccgcct
     5581 cgaagcgctc cagcaagtcg ggcgacttca gcccggcgag cacctcgcgg tgcagccgcg
     5641 cca
//
LOCUS       DASYJA010000377         2372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_875433, whole genome shotgun sequence.
ACCESSION   DASYJA010000377 DASYJA010000000
VERSION     DASYJA010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875433"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VGO02_08315"
     CDS             <1..243
                     /locus_tag="VGO02_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEV7477727.1"
                     /translation="PKAWLVATSAAGTYLQAGASPLAQALWMTALFLAAALPSGFVWL
                     AFGTALRRWLSSPRRLRVFNVAMGVLLAASVTPLVL"
     gene            complement(224..1450)
                     /locus_tag="VGO02_08320"
     CDS             complement(224..1450)
                     /locus_tag="VGO02_08320"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7477728.1"
                     /translation="MHVLRLRDYRLYLCGRFFAAMSQQMLAVAIGWYLYDLTGDPMTL
                     GYAGLATFVPIALFTLPGGDLADRFDRRQILALAHLVQATCSAVLVYLAMTRTVTPWP
                     FYAVLAASGMARAYSAPAVQSFAPFLVPREQFAESVAWSASANRVATIVGPALGGLIY
                     LLGPEAAFAACLVQSLLVSGSMISIRTTVAPAVEKGSTGIQRAIAGLRYVRRQPVVLG
                     AITLDLFAVTLGSITALLPVYARDVLDVGPGGLGAMRSSLALGAVATALLIAALPARR
                     LPHAGRAMFVGVAVFGAAILAFGVSTSFALSIAALAVMGAADMISVWVRSTVVQLATP
                     DDMRGRVSAVHMLFVGTSNEFGDFRAGAVAAWLGALPAVMAGGICTLLVTALCARFFP
                     ELLRIDRLAEVTALKG"
     gene            complement(1451..>2372)
                     /gene="asnS"
                     /locus_tag="VGO02_08325"
     CDS             complement(1451..>2372)
                     /gene="asnS"
                     /locus_tag="VGO02_08325"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004244664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEV7477729.1"
                     /translation="HTPIITTSDAEGAGAMFRVSTLDLANLPRTPEGKVNWAEDFFGK
                     EASLTVSGQLNVEAYCLALSKVYTFGPTFRAENSNTSRHLAEFWMIEPEIAFADLSDD
                     ATLAEELLKYIFKSLLNERPEDMTFFDDRIEKGLVAKLEGIVTSEFVRMDYSEAIAVL
                     ERAKEKFEFPVKWGIDLQSEHERYLTEKHAKKPVIVMNYPKAIKAFYMRQNDDGKTVA
                     AMDVLAPGIGEIIGGSQREERLDRLDARMEEMSIKRENYEWYRDLRRYGTVPHAGFGL
                     GFERTIAYVTGLSNVRDAIPFPRTPGNAKY"
BASE COUNT          407 a          816 c          774 g          375 t
ORIGIN      
        1 ccgaaggcct ggctggtcgc gaccagcgcg gccgggacct atctccaggc aggcgcgagc
       61 ccgctggccc aggcgctatg gatgacggcg ctgttcctcg ccgcggcgct gcccagcggc
      121 ttcgtctggc tcgcgttcgg caccgcgctg cggcgctggc tgagctcgcc gcgccgcctg
      181 cgcgtcttca acgtcgcgat gggcgtgctg ctcgccgctt cggtcacccc tttagtgctg
      241 tgacctcggc cagccggtcg atgcgcagca gctcgggaaa gaagcgcgcg cagagcgccg
      301 tgaccagcag cgtgcagatg ccgccggcca tcaccgccgg cagcgcgccg agccaggccg
      361 ccaccgcgcc ggcgcggaaa tcgccgaact cgttcgaggt gccgacgaac agcatgtgca
      421 cggcgctgac acggccgcgc atgtcgtcgg gcgtggcgag ctgcaccacg gtggacctca
      481 cccagacgct gatcatgtcc gccgcgccca tgaccgcgag cgcagcgatc gacagcgcaa
      541 agctggtgga cacgccgaac gccaggatcg ccgcaccgaa cacggccacg ccgacgaaca
      601 tcgcccggcc ggcgtgcggc agcctgcgcg ccggcagcgc cgcgatcagc aacgcggtgg
      661 ccaccgcgcc gagcgcaagg ctgctgcgca tcgcacccag tccgcccggc ccgacatcca
      721 gcacgtcgcg cgcatagacg ggcaataagg cggtaatgct tcccagcgtt accgcaaaca
      781 aatcgagggt gatcgcgccg agcaccaccg gctggcggcg tacgtagcgc agacccgcga
      841 tggcgcgttg aataccggtg ctgccctttt ccactgccgg ggcgacggtc gtgcggatcg
      901 agatcatcga gcccgacacg agcagcgact ggaccaggca ggcggcgaag gccgcttccg
      961 gccccagcag gtagatcagg ccgccgagcg ccggtccgac gatcgtcgcc accctgttcg
     1021 ccgatgcgct ccacgccacc gattcggcga attgctcgcg cggcacgaga aacggcgcga
     1081 aggactgcac cgccggcgcc gagtaggcgc gcgccatgcc ggaagccgcc agcaccgcat
     1141 agaacggcca cggcgtgacc gttcgcgtca tcgccaggta aacgagtacc gccgagcacg
     1201 tggcctgcac gaggtgcgcg agggcgagga tctgccggcg gtcgaagcgg tccgcgaggt
     1261 cgccgccggg cagcgtgaac agcgcgatcg gcacgaaggt ggcgagcccg gcgtagccaa
     1321 gggtcatcgg atcgccggtg aggtcgtaca ggtaccagcc gatcgctacc gcgagcatct
     1381 gctgcgacat cgccgcgaag aaccgcccgc acaggtaaag gcggtaatcg cgcagcctca
     1441 ggacgtgcat ttagtacttg gcgttacccg gcgtgcgcgg gaacgggatc gcatcgcgca
     1501 cgttggaaag accggtcacg taggcgatgg tgcgctcgaa gccgaggccg aatccggcgt
     1561 gcggcacggt gccatagcgg cgcaggtcgc gataccactc gtagttctcc ctttttatgc
     1621 tcatttcttc cattcgcgca tcgaggcgat ccagccgctc ctcgcgctgg ctgccgccga
     1681 tgatctcgcc gatgcccggc gcgagcacgt ccatcgccgc caccgtcttg ccgtcgtcgt
     1741 tctggcgcat gtagaacgcc ttgatcgcct tcgggtagtt catgacgatc accggcttct
     1801 tcgcgtgctt ctcggtgaga tagcgctcgt gctccgactg caggtcgatg ccccacttca
     1861 ccgggaactc gaacttctcc ttggcgcgct caagaacggc gatcgcctcc gagtagtcca
     1921 tgcggacgaa ttccgaggtg acgatgccct cgagctttgc caccaggccc ttttctatcc
     1981 tgtcatcgaa aaacgtcatg tcctccggtc gctcgttcag cagcgacttg aatatgtact
     2041 tgagtaactc ctctgccaga gtggcatcgt ccgacaggtc ggcgaaggcg atctcgggct
     2101 cgatcatcca gaattccgcc aggtggcggc tggtgttcga gttctcggcg cgaaaggtcg
     2161 ggccgaaggt atagaccttc gacagcgcga ggcagtaggc ctcgacgttg agctgcccgg
     2221 agacggtcag gctcgcttcc ttgccgaaaa aatcctcggc ccagttgacc ttcccctcag
     2281 gcgtgcgcgg caggttcgcc agatccagcg tactgacgcg gaacatcgcg cccgcgcctt
     2341 ccgcatccga agtcgtaatg atcggcgtgt gc
//
LOCUS       DASYJA010000378         8571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_146899, whole genome shotgun sequence.
ACCESSION   DASYJA010000378 DASYJA010000000
VERSION     DASYJA010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8571
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146899"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VGO02_08330"
     CDS             <1..693
                     /locus_tag="VGO02_08330"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7477730.1"
                     /translation="LDAARALQDAKRVSLGFLYWLQTEAPATAARLGAPEIRLRPDVM
                     GSADGLSKHPYIRESRRIKAKKTIVEQEVSEHHQKGPRAAHFADSVGVGWYPVDIHRS
                     GPEDVGASCRTKPFQIPLGALLPVRIANLIAGAKNIGTTHITNGCYRLHPVEWNIGEA
                     AGALAAFALRERASPAAVREDAGARERFQRSLLDEGVPLGWVTGTGVGEARFVSEQLK
                     FMMEAVREGVLP"
     gene            690..1592
                     /locus_tag="VGO02_08335"
     CDS             690..1592
                     /locus_tag="VGO02_08335"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEV7477731.1"
                     /translation="MTPQQVLALKPKVLTQAQRQSYFRDGFLLVERAVGQDWIGKLRA
                     ATDELIERSRRIAKSDPVFDLEPKHRPDAPRLRRVSNPVEQHAVFWEYCLNSAMADIV
                     ADLVGPDVKFHHSKLNFKWANGGEEVKWHYDISFYPHTNYSPLTVGTYLYDCGMEQGP
                     LACLPASHQIEPMLSQYNAEGSWTGCLSDADVAKLDLSKMKYLPGPAGCLTIHNCRTL
                     HYSAKNDSDLGRPLLLNAMSSADAFPYTVNPIKPKHDQYILRGKRAQFAHHDPRPCLM
                     PPDWSKGYTSIFSLQQEEEAEIAR"
     gene            1632..3185
                     /locus_tag="VGO02_08340"
     CDS             1632..3185
                     /locus_tag="VGO02_08340"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEV7477732.1"
                     /translation="MNRREFLAIGAASIGAACSLPPARAPAAASQAVPLSAVRKLVVE
                     LVDPLLGRCQYVADTGTQRIARPILLARRGDRFDAVIENRLPQPTTIHFHGLTLSEAQ
                     DGAGFDPIPPGGSKPLAFRIRNRSGLYWFHPHPHGFTAEQVYSGLVGLVVVGDEDDAA
                     LDQAMALSPTNRLALAIADVRVAGGALRPYAPSSEDCHTGWLGNRVMVNGAIEARYPV
                     APGWVRLQLLNACNARGLLLAFRAADLPVPFFLLGTDGGLLSAPRALDRVFLHPAERV
                     DVVLDARPHGSVDAISLEYDPRRHAPAPMIARPHPARARFPALAAENICGPAGARPAG
                     EVVAEGERLPLFSLTVEGEHLPPRALPQRLSSLAEATPPLDAGARRMRLDLSEQRGFL
                     IDNQPYELDEIAFSAARGAREVWEVRNSPVSMPHPMHLHGFGFRVLRRQGTFGPARAL
                     AGESAGRLPTDLGVKDTVVVWPNETLWLAVDFTLPDEAAFRGRQRYMFHCHNLEHEDG
                     MMMRNFAVL"
     gene            complement(3193..4131)
                     /locus_tag="VGO02_08345"
     CDS             complement(3193..4131)
                     /locus_tag="VGO02_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEV7477733.1"
                     /translation="MTRILACFILVSMTTPADAGLALVGNENGGTISLIDTAKDEVVG
                     EIKTGGKPRGTAIHAARKLAYVSDQPSNALLVIDLDKRAVVKKIDLGESPEGVYISPD
                     GKLVAAAVELTSSVAFIDTASDAVVFRVKIKGDNPEHAVFSPDGRFVYVSAEEDDKLE
                     VIDVAARTLVAQLKVGTRPRGIAFTPDGAKAYVACERADTVYVLDARQHKILRTIKVG
                     LRSNGLAMHPGGKRLYLSNGGAANVMVIDTGTDQVVATIPVGQRPWNMALTRDGAKLY
                     VANGRSDSVSVIDTAKGERIKDIPVGKTPWGVHILD"
     gene            complement(4193..6454)
                     /locus_tag="VGO02_08350"
     CDS             complement(4193..6454)
                     /locus_tag="VGO02_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV7477734.1"
                     /translation="MDVVEVIAPTPVPGLGVPRDRLPANVQILRSQELEDAQAVNVPE
                     VMRLRLPSVNVNEIQSNPFQPDVSFRGFTASPLLGTPQGISIYVDGVRVNEPFGDVVN
                     WDLIPMEAIDSMVLVPGSNPLFGLNTLGGALSLRTKSGERNPGGEIELSGGSFGRLSG
                     EFSYGRRLAQGMHFFAAGTWFEEDGWRDYSPSKVKQLFAKVGRRSGNFEWDLGLTAAD
                     TDLTGNGLVPQSIHDQRREAIFTRPDRTRNELGMLSFNGSWYLSDNARLSTVLYYRRV
                     KTRTLNGDINDGFEAGPNDATGGGTGLDSGTSASHRTSTRQEAYGGALQWSADHRNHR
                     IALGTAADFGRSKFEQSTALGVFDATRAVVETDPEALENALDGRTRTMSVFATDTWSL
                     RPNLHLTLSARYNTTTVELRDTGPSAPALDGEHSFSKLNPAAGVAYQASRALTWYAGF
                     NQGNRAPTPIELGCADPARPCTLPNALAADPPLNQVVARTFELGARGRLAGDLRWNAG
                     VFQTTNTDDILFVGTTTSAGFFTNFGKTRRRGIELGLSGSTGALQWNAAYSFVRATFE
                     SSACIVSENNSSRGTSAECSPEDSGNPGTFLGDGLIAVNPGNRIPGIPEHSFKLALWL
                     PVAERLLLGADLVAFSSQYVRGNENNRHEAGTAEDLNGDTRTFLGPGKVAGYGLVNLR
                     ARYNLLQGWELFARINNLFDRRYATAGALAENPFDSAGRFQTNSDDWTRETFIAPGAP
                     RAGWIGVRYRFKE"
     gene            complement(6570..7097)
                     /locus_tag="VGO02_08355"
     CDS             complement(6570..7097)
                     /locus_tag="VGO02_08355"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEV7477735.1"
                     /translation="MSPRYTRTAIALHWVLAALLLGQLALGWSMIEIPKEPVGVRAWW
                     FNLHKSVGLSIGLLMLARLGWRAAHPAPPLPAELPRWQALAARANHALLYACLLVMPL
                     SGYLGSSFTKYPIKVYGATLPHWGWEAPGLKELCSLVHYATVWILMTLVAVHVGAALK
                     HLLVARDGVFWRMWP"
     gene            complement(7094..7753)
                     /locus_tag="VGO02_08360"
     CDS             complement(7094..7753)
                     /locus_tag="VGO02_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEV7477736.1"
                     /translation="MFPARTLGVLLVCATHTIALAQQGAININAELLQAARRGSEPEV
                     RALLEKGASLESRNRLGDTPLNVAAKNGHTALGVMLIERGANVNQGNLAGVTPLMSAA
                     YNGDLDLVRALLRRKADLAPVDRMKKTALLYAAANGHEQVLGALLEQGVDVNARYDNE
                     LTALMWAAGFGRDQAVKLLISRGADLNARDNRGKTALAIAEEEGRQATAGLLRAAGAQ
                     K"
     gene            7832..>8571
                     /locus_tag="VGO02_08365"
     CDS             7832..>8571
                     /locus_tag="VGO02_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEV7477737.1"
                     /translation="MTKNTRRSLAIAAATLGALALAVPAFGQEVKPAQATVGGEVPKG
                     INVSQDQLTKAGNDSRNWLHTNGNYAQTRYYPGTAINTQNVRSLRPVFVFQTEVVESM
                     ETAPIVIDGVMYLTTAFNHVYAADAASGKEFWHYKHKMGPVTTFCCGPNNRGVAISGG
                     KVFMGTLDAKLVALDAKSGKVLWETQIEDPEKGYSETMSPTVIENKVLIGTNGGEYGI
                     RGFVKAYDADSGKLLWTFYTIPEKGHEGV"
BASE COUNT         1381 a         2843 c         2909 g         1438 t
ORIGIN      
        1 ctcgatgccg cccgagcctt gcaggacgcg aagcgggtaa gcctcggctt cctgtattgg
       61 ctgcagactg aagccccggc gacggcagcg cgcctcggcg cgcccgagat acgtctgcgt
      121 cccgatgtca tgggtagcgc ggacggcctc tccaagcatc cgtacatccg cgagtcgcgg
      181 cgcatcaagg cgaagaagac gatcgtcgag caggaagttt ccgagcacca ccagaaaggg
      241 ccgcgcgcgg cgcatttcgc cgattccgtc ggagtcggct ggtacccggt cgacatccac
      301 cgctccggcc ccgaagacgt cggggcaagc tgccgcacca agccgttcca gattcccctc
      361 ggcgcactgc tgccggtgcg catcgccaat ctcatcgccg gcgcgaagaa catcggcacc
      421 acgcatatca ccaacggctg ctaccggctg cacccggtcg aatggaacat cggcgaggca
      481 gcgggggcgc tggcggcttt cgcgttgcgg gagcgcgcct cgcccgctgc agtgcgggag
      541 gatgccggag cccgggagcg ctttcagcgc agcctgctgg acgaaggggt cccgctcggc
      601 tgggtcacgg gtacgggcgt cggcgaggcg agattcgtct ccgagcaatt gaagtttatg
      661 atggaggctg ttcgcgaagg agtcttgcca tgacaccgca acaggttctc gccctcaagc
      721 cgaaggtcct gacgcaggcg cagcgccagt cctatttcag ggacggcttc ctgcttgtcg
      781 agcgcgcggt cggtcaggac tggatcggca agctgcgcgc cgcgacggac gagctgatcg
      841 agcgcagccg caggatcgcc aagtcggacc cggtcttcga cctcgagccc aagcaccggc
      901 cagacgcgcc gcgcctgcgc cgggtgtcga acccggtcga gcagcacgcc gtgttctggg
      961 agtactgcct caattcggcg atggcggaca tcgtcgctga cctggtcggt ccggacgtga
     1021 agttccacca ctccaagctc aatttcaaat gggcgaacgg cggcgaggag gtgaagtggc
     1081 actacgacat cagcttctac ccgcacacca attattcgcc gctgacggtc ggcacctacc
     1141 tgtatgactg cggcatggag cagggtccgc tcgcttgtct gcccgcaagc caccagatcg
     1201 agccgatgct ctcccaatac aacgccgaag gcagctggac cggatgcctg tcggacgccg
     1261 atgtggcgaa gctggacctg tcgaagatga agtacctgcc cggcccggcg ggttgcctga
     1321 cgattcacaa ctgccgcacg ctgcactatt cggcgaagaa cgattcggac ctcggccggc
     1381 cgctgctgct gaacgcgatg agctccgccg acgcgtttcc ctacacggtc aacccgatca
     1441 agccgaagca cgaccagtac atcctgcgcg gcaagcgcgc gcagttcgcg caccatgacc
     1501 cgcggccctg cctgatgccg ccggactggt cgaagggcta tacctcgatc ttctcgctgc
     1561 agcaggaaga agaggcggaa atcgcgcgct agcgttaggt ttgccctggc ggcgagtcaa
     1621 ggcaggtccg aatgaaccga cgtgaattcc tggcgatcgg cgccgcgtcg atcggcgcgg
     1681 catgcagctt gcctcccgcg cgcgcccctg ccgcggcgtc gcaggccgtg ccgctcagcg
     1741 cggtgcgcaa gctggtcgtc gagcttgtcg atccgctcct tggccgctgc cagtacgtgg
     1801 cagataccgg gacgcagaga atcgcccgac ccattcttct cgcgcggcgc ggtgatcgct
     1861 tcgacgccgt gatcgagaat cggttgccgc agcccacgac cattcacttt cacggcttga
     1921 cgctctccga agcccaggac ggagcgggtt tcgatccgat ccctccgggc ggatcgaaac
     1981 cgctcgcctt ccggatccgc aatcgcagcg ggctttactg gtttcacccg cacccgcacg
     2041 gattcaccgc ggaacaggtt tactcggggc tcgtcggtct ggtggtcgtc ggcgacgaag
     2101 acgacgccgc gctcgaccaa gcgatggcgc tttcgcccac caaccggctc gcgctcgcga
     2161 tcgcggacgt gcgcgtcgcc ggcggcgcct tgcgccccta tgctccgagc agcgaggact
     2221 gccataccgg ctggctgggc aatcgagtca tggtgaacgg ggcgattgag gcgcgatatc
     2281 cggtcgcgcc gggctgggtg cggcttcagc tcctcaacgc gtgcaatgca cgcggcctgc
     2341 tccttgcgtt tcgcgcggct gatttgcccg taccgttctt cctgctcgga accgatggcg
     2401 ggctcctttc cgctccgcga gcgctcgatc gcgtcttcct gcacccggcc gagcgcgtcg
     2461 acgttgtgct ggacgcccgt cctcacggga gcgtcgatgc gatcagcctc gaatacgacc
     2521 cgcgccggca cgcgccggcg ccgatgattg cgcgtccgca tccggcgcgc gcccgctttc
     2581 ccgccctcgc cgcggagaac atctgcgggc cggcaggtgc gcgccccgcg ggcgaagtcg
     2641 tggccgaagg cgagcggctg ccgcttttct cactgaccgt ggagggcgag catctgccgc
     2701 cgcgggcttt gccgcagcgc ctgtcgtcgc ttgcggaagc gacgccgccg ctggacgccg
     2761 gcgcacggcg aatgcggctc gacttgagcg agcagcgcgg ctttctcatt gataaccagc
     2821 cctatgagct ggacgagatc gcgttcagcg cggcgcgcgg cgcgcgcgag gtgtgggaag
     2881 tcaggaacag tcccgtctcg atgccgcatc cgatgcacct gcacggcttc ggctttcgcg
     2941 tattgcggcg ccaggggacg ttcgggccgg cgcgcgcgct ggcgggggag tccgcggggc
     3001 ggctgccgac cgatctcggg gtgaaggaca ccgtggtcgt ctggccgaac gaaacgctat
     3061 ggctggcggt ggatttcacg ctgccggacg aagctgcgtt tcgcggccgg cagcgctaca
     3121 tgttccattg ccacaacctc gagcacgagg acggcatgat gatgcgcaac ttcgccgtgc
     3181 tctgagcgac gctcagtcga ggatgtgcac gccccagggc gtcttgccga ccgggatgtc
     3241 cttgatgcgc tcgccctttg cggtatcgat gaccgatacg ctgtccgagc ggccgttcgc
     3301 cacgtagagc ttcgcgccgt cgcgggtcag cgccatgttc cagggccgct ggccgaccgg
     3361 gatcgtcgcg acgacctggt cggtcccggt gtcaatcacc atcacgttgg cggcgccgcc
     3421 gttgctcagg tacaggcgct tgccgcccgg atgcatcgcc agaccgttgg agcgcaggcc
     3481 gactttgatg gtgcgcagga tcttatgctg gcgcgcgtcg agcacgtaga cggtgtcggc
     3541 cctttcgcag gcgacgtacg ccttcgcgcc gtcgggcgtg aaggcaatgc cgcgcgggcg
     3601 cgttccgacc ttgagctgcg cgaccagcgt gcgcgccgcg acgtcgatga cttcgagctt
     3661 gtcgtcctcc tcggcgctga cgtagacgaa gcggccgtcg ggcgagaaca ccgcgtgctc
     3721 aggattatcg cccttgatct tgacgcgaaa gaccaccgca tcgctcgcgg tatcgatgaa
     3781 cgccacgctg ctggtgagct cgaccgcggc tgcgaccagc ttgccgtcgg gcgagatgta
     3841 gacgccctcg ggcgattcgc cgaggtcgat cttcttcacc acggcgcgct tgtcgaggtc
     3901 gatcacgagc agcgcgttgc tcggctgatc ggacacgtac gccagcttgc gcgcggcgtg
     3961 gatcgccgtg ccgcgcggct tgccgccggt cttgatctcg ccgacgacct cgtccttggc
     4021 ggtgtcgatc agcgaaatcg tgccgccgtt ctcgttgccc acgagggcaa gccccgcgtc
     4081 ggcgggagtc gtcatcgata ccaggatgaa acaagcaaga atgcgtgtca tcatcgtaat
     4141 gctccggtca aaccccagtg aaatggtgca tcgctagtct agcgctcgtg ggtcactcct
     4201 tgaagcggta gcgcaccccg atccagcccg cgcgcggcgc gccgggcgca atgaaggtct
     4261 cgcgcgtcca gtcgtctgag ttggtctgga accggccggc tgaatcgaac gggttctcgg
     4321 ccagcgctcc ggcggtggcg tagcggcggt cgaaaagatt gttgatgcgc gcgaagagct
     4381 cccatccctg gagcaggttg tagcgcgcac gcaggttcac gaggccatag ccggcgacct
     4441 tgcccggccc gaggaaggtg cgggtatcgc cgttcaaatc ctcggcggtg ccggcctcgt
     4501 gccggttgtt ctcgttgccg cgcacgtatt ggctcgagaa cgccaccagg tccgcgccga
     4561 gcagcaggcg ctccgcgacc ggcagccaga gcgcgagctt gaagctgtgc tcagggatgc
     4621 cgggaattcg attcccgggg ttgaccgcga tcagaccgtc gccgaggaag gtgccgggat
     4681 tgcccgagtc ctctggcgag cattcggccg aggtgccgcg gctcgagttg ttctcggaca
     4741 cgatgcaggc gctcgattcg aaggtcgcgc gcacgaagct gtaggcggcg ttccattgca
     4801 gggcgccggt cgagcccgag aggccgagct ctatgccgcg gcggcgagtc ttgccgaagt
     4861 tggtgaagaa gcccgcgctg gtggtagtgc cgacgaacag gatgtcgtcg gtgttcgtgg
     4921 tctggaagac gcccgcgttc cagcgcaggt cgccagcgag gcggccgcgc gcgccgagct
     4981 cgaaggtgcg ggcgaccacc tgattgagcg gcggatcggc cgcgagcgcg ttcggcagcg
     5041 tgcacgggcg ggcggggtcg gcgcagccga gctcgatcgg cgtcggtgcg cgattgccct
     5101 ggttgaagcc ggcgtaccag gtgagcgccc gggacgcctg gtatgccacg ccggcagccg
     5161 ggttgagctt gctgaagctg tgctcgccgt cgagcgccgg cgccgagggc ccggtgtcgc
     5221 gcagctcgac cgtggtggtg ttgtagcgcg cggacagggt gagatgcaga ttcggccgca
     5281 gcgaccaggt gtcggtcgcg aacacgctca tcgtgcgggt acgcccgtcc agggcgtttt
     5341 ccagcgcttc cgggtcggtc tcgaccaccg cccgcgttgc gtcgaacacg cccagcgccg
     5401 tgctctgctc gaacttgctg cggccgaaat cggccgccgt gccgagagcg atccggtggt
     5461 tcctgtggtc tgcgctccat tgcaacgcgc cgccgtaggc ttcctggcgg gtcgaggttc
     5521 tgtggctggc gctggtgccg ctgtcgaggc cggtacctcc gccggtggcg tcgttcgggc
     5581 ccgcctcgaa accgtcgttg atgtcgccgt tcagggtgcg cgtcttgacg cgcctgtaat
     5641 agaggaccgt ggacaggcgc gcgttgtcgg agaggtacca gctcccgttg aagctcagca
     5701 ttccgagctc gttgcgggtg cggtccggac gcgtgaagat cgcctcgcgg cgctgatcgt
     5761 ggatcgattg cggcaccagc ccgttcccgg tgaggtccgt atcggccgcc gtcaggccga
     5821 gatcccattc gaagttgccc gagcgccggc ctaccttggc gaagagctgc ttgaccttcg
     5881 agggcgagta gtcgcgccag ccgtcttctt cgaaccaggt accggcggcg aagaaatgca
     5941 ttccctgcgc gaggcgccgg ccgtaactga actcgcccga aagccgcccg aacgagccgc
     6001 cggagagctc gatttctccg ccgggattgc gctcgccgct tttcgtgcgc agcgagagcg
     6061 cgccgccgag cgtgttcagt ccgaacagcg gattcgagcc cggtaccagg accatgctgt
     6121 cgatcgcctc catcgggatc aagtcccagt tcaccacgtc gccgaacggc tcgttgacgc
     6181 gcacgccgtc cacgtagatc gagatgcctt gcggcgtgcc gaggagcggc gaggcggtga
     6241 agccgcggaa gctcacgtcg ggctggaagg ggttgctctg gatttcgttg acgttgacgc
     6301 tcggcagcct cagcctcatc acctcgggaa cgtttacggc ctgcgcgtcc tcgagctctt
     6361 gcgagcgcag gatctggacg ttcgccggca agcggtcgcg cggcaccccg agcccgggca
     6421 ccggcgtcgg cgcaatgacc tccacgacgt ccaccgtcgg cagcatcgtt gcagattgct
     6481 gcgctgcggc ggtacccgcc cctgcgtaca gtaccgctgc gagaacgtgt tttacccgac
     6541 aacaccagcg ggacccccgc gccggcacgc tatggccaca tgcgccagaa cacgccatcg
     6601 cgcgcgacga gcagatgctt cagcgccgcc cccacgtgca ccgcgacgag cgtcatgaga
     6661 atccacaccg tggcatagtg caccaggctg catagctcct tcagtcccgg agcttcccag
     6721 ccccagtggg gaagcgtggc gccgtagacc ttgatcgggt atttggtgaa cgacgagcca
     6781 agatatccgg acagcggcat cacgagcagg caggcataga gcagggcgtg attggccctc
     6841 gccgcgagcg cctgccagcg cggcagctca gccggaagcg gtggcgccgg atgcgcggcg
     6901 cgccagccaa gccgcgcgag catcagcagc ccgatggaga gcccgacgga cttgtgcagg
     6961 ttgaaccacc aggcacgcac gcccacgggc tccttcggaa tctcgatcat cgaccagcca
     7021 agcgcgagct gcccgagcag cagcgccgcc aggacccagt gcagcgcgat ggcggtgcgg
     7081 gtataccgtg ggctcacttc tgcgcgccag cggcgcgcag caggcccgcg gtggcctgcc
     7141 gaccctcctc ttccgcgatt gcaagcgcgg ttttgccgcg gttgtcccgg gcattgaggt
     7201 cggccccgcg cgagatcagc agcttcaccg cctggtcccg accaaagccg gcggcccaca
     7261 tcagggccgt cagctcgttg tcatagcgcg cgttgacgtc cacgccctgc tcgagcagcg
     7321 cgcccagcac ttgctcgtgc ccattggcgg ccgcgtacag cagcgcggtc ttcttcatcc
     7381 ggtcgacggg agcgaggtcg gccttgcggc gcaggagcgc gcgcaccagg tcgaggtcgc
     7441 cgttgtacgc cgcgctcatc agcggggtca cccccgccag gttcccctgg ttgacgttgg
     7501 cgccgcgctc gatgagcatc acgccgagcg cggtatggcc gttctttgcg gcgacgttca
     7561 gcggcgtgtc gccgagccgg ttgcgcgatt ccaggctcgc ccccttctcc agcagtgcgc
     7621 gcacctccgg ctcgctgccg cggcgcgccg cctgaagcag ctcggcgttg atgttgattg
     7681 cgccctgctg ggcgagggcg attgtgtgcg tcgcacaaac gagcaggacg cccagcgttc
     7741 tggcgggaaa cactcgctct cctgttgaac cggcgtcgcc cgagggttag actggccgag
     7801 gaaatcttag cagagcccaa aggagacgac tatgacgaag aacaccaggc gctcgcttgc
     7861 aatcgccgct gctactctcg gcgccctcgc gctcgctgtg cccgcattcg ggcaggaagt
     7921 gaagccggcg caggcaaccg tcggcggcga agtgcccaag ggcatcaacg tgtcgcagga
     7981 ccagctgacc aaggccggca acgactcgag gaactggctg cataccaacg gcaactacgc
     8041 gcagacccgg tactacccgg gcacggcgat caatacgcag aatgtccgaa gcctgcggcc
     8101 ggtgttcgtg ttccagaccg aggtggtcga gtcgatggaa accgcgccga tcgtgatcga
     8161 tggcgtgatg tacctcacga ccgcgttcaa ccatgtgtac gcggccgatg cggcgagcgg
     8221 caaggagttc tggcactaca agcacaagat ggggccggtg acgaccttct gctgcggccc
     8281 gaacaaccgc ggcgtcgcca tctcgggcgg caaggtcttc atggggacgc tggacgccaa
     8341 gctcgtggcg ctcgacgcga agagcggcaa ggtgctgtgg gagacgcaga tcgaggaccc
     8401 ggaaaagggc tacagcgaga ccatgtcgcc caccgtgatc gagaacaagg tgctgatcgg
     8461 caccaacggc ggcgagtacg gcatccgcgg cttcgtcaag gcttacgacg ccgactcggg
     8521 caagctgctg tggaccttct acacgatccc cgagaagggc cacgagggcg t
//
LOCUS       DASYJA010000379         2428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1509614, whole genome shotgun sequence.
ACCESSION   DASYJA010000379 DASYJA010000000
VERSION     DASYJA010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509614"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="VGO02_08370"
     CDS             complement(<1..1031)
                     /locus_tag="VGO02_08370"
                     /inference="COORDINATES: protein motif:HMM:NF000658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEV7477738.1"
                     /translation="MGELAFDNTFVRDLPADPVSDNLPRQVSGASYTRVAPTPVTAPR
                     LLAWSDELGERLGIGRPDATMVEVLAGNRVLPGMQPYAARYGGHQFGHWAGQLGDGRA
                     ITLGEAFARDGTRQELQLKGAGKTPYSRTADGRAVLRSSLREFACSEAMHWLGVPTTR
                     ALSLVATGESVIRDMFYDGNPAPEPGAVICRVAPSFLRFGNFQIHAARSEPELLKQLV
                     DYVMKLHFQKIKTYEEFFSEVCARTARLMVDWMRLGFVHGVMNTDNMSILGLTIDYGP
                     YGWLEGFDTQWTPNTTDAPTLRYIYGNQAAIAHWNLARLAEALVALIDKKHLEDGLAL
                     FADTFQSGW"
     gene            1050..2171
                     /locus_tag="VGO02_08375"
     CDS             1050..2171
                     /locus_tag="VGO02_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEV7477739.1"
                     /translation="MSNADAVALTRSLLGFETVNPPGRERDCARYAGAMLQEWGFQVD
                     YHEYADSRTSVVARLGGSQEKAPLCLTGHLDIVPLGARPWTKDPFRGETDGDKLYGRG
                     SSDMKAGVAAILLAARSFSKKLSNTPGIVIVLTAAEEGGCIGSRHLAQTRLLGKAGAM
                     VVGEPTSNYPLVGHKGSVKFYAAFKGVGAHGSMPELGVNAIYKAARAIAKLEDFSFGV
                     EKHSVMGGPTLNVGTIEGGNTVNAVPDAARIGVDIRTVPGMNHDELLSRLKRLIGEAE
                     IEVFSNLPPVWTAPDEDWMQRVFEICTPYVGEAPAARTAPYMTDAANLLKVYTGAPTV
                     VLGPGEAAMAHQTDEYCSMERLRQSVEIYEKIISDWCRV"
     gene            2237..>2428
                     /locus_tag="VGO02_08380"
     CDS             2237..>2428
                     /locus_tag="VGO02_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477740.1"
                     /translation="MSGEQSPQFAWHDTPTRLRTALKQDEFVLFCQPILALRGGGGFA
                     LAEVLIRLREEEQALLPPGE"
BASE COUNT          402 a          789 c          843 g          394 t
ORIGIN      
        1 tcccatccgc tctggaaggt atcggcgaac agcgccagcc cgtcttccag atgctttttg
       61 tcgatcaagg caaccagggc ttcggccagg cgcgcgagat tccagtgcgc aatcgccgcc
      121 tggttgccgt agatgtagcg cagcgtcggc gcgtcggtgg tattcggcgt ccattgcgtg
      181 tcgaagccct cgagccagcc gtaggggcca tagtcgatgg tgaggccgag gatcgacatg
      241 ttgtcggtgt tcatcacgcc gtgcacgaag cccaggcgca tccagtcgac catgagccgc
      301 gcggtacgcg cgcacacctc ggaaaagaat tcctcataag tttttatttt ttgaaaatga
      361 agcttcatta cgtagtccac cagctgcttc agcagctcgg gctcgctgcg cgcggcgtgg
      421 atctggaagt tgccgaagcg caggaacgag ggcgcgacgc ggcagatcac ggcgccgggc
      481 tcgggcgccg gattgccgtc gtagaacatg tcgcggatca cgctctcgcc ggtggccacc
      541 aggctgagcg cgcgcgtcgt cggcacgccg agccagtgca tcgcttcgct gcaggcgaac
      601 tcgcgcagcg acgagcgcag caccgcgcgg ccgtcggcgg tgcgcgagta gggggtcttg
      661 ccggcgccct tgagctgcag ctcctggcgc gtgccgtcgc gcgcgaacgc ttcgcccagc
      721 gtgatggcgc ggccgtcgcc gagctggccc gcccagtggc cgaactggtg gccgccgtag
      781 cgcgcggcat acggctgcat gccgggcagc acgcggttgc cggcgagcac ctccaccatc
      841 gtggcgtccg ggcgcccgat gcccaggcgc tcgcccagct cgtcggacca ggcgagcagg
      901 cgcggcgccg tcaccggcgt cggcgccacg cgcgtgtacg aagcgccgct gacctggcgg
      961 ggcaggttgt cgctcaccgg atcggcgggc aggtcgcgca cgaacgtgtt gtcgaatgcg
     1021 agctcgccca taggtcgtat catctcatca tgagcaatgc ggatgccgtg gcgctgacca
     1081 ggtctttgct cggcttcgag acggtcaatc cccctgggcg cgaacgggat tgcgcgcgct
     1141 acgccggggc gatgctgcag gagtggggct tccaggtcga ctatcacgag tacgccgaca
     1201 gccgcaccag cgtggtcgcg cgcctgggcg ggtcgcagga gaaggcgccg ctgtgcctca
     1261 ccggccacct cgacatcgtg ccgctcggcg cgcgcccctg gacgaaggac ccgttccgcg
     1321 gcgagaccga cggcgacaag ctctatggcc gcggctcttc ggatatgaaa gccggggtgg
     1381 cggccatcct tcttgcagct cgctcgtttt caaaaaagtt aagtaatacc cctggaatcg
     1441 tgatcgtcct tacggcagct gaggagggcg gctgcatcgg ctcgcgccac ctcgcgcaga
     1501 cccggctgct cggcaaggcc ggcgccatgg tggtgggcga gccgacctcg aactacccgc
     1561 tggtcggcca caagggctcg gtcaagttct acgccgcctt caagggcgtc ggtgcgcacg
     1621 gctcgatgcc cgagctcggc gtcaacgcga tctacaaggc ggccagggcg atcgccaagc
     1681 tggaagattt ttcttttggc gttgaaaagc attccgtgat gggcgggccg acgctcaacg
     1741 tcggcacgat cgagggcggc aacacggtga acgcggtgcc cgacgcggca cgcatcggcg
     1801 tcgatatcag gaccgtgccc gggatgaatc atgacgagct gctttcgcgc ctgaagaggc
     1861 tgatcggaga agccgagatc gaggttttct ccaacctgcc tcccgtctgg accgcgcccg
     1921 acgaggactg gatgcagcgc gtgttcgaga tctgcacgcc gtacgtcggc gaggcgccgg
     1981 cggcgcgcac cgcgccgtac atgaccgacg cggcgaacct gctcaaggtc tacaccggcg
     2041 cgccgacggt ggtgctcggg ccgggcgagg cggcgatggc gcaccagacc gacgagtact
     2101 gcagcatgga gcggctgcgc cagtcggtcg aaatatacga aaagataatt tccgactggt
     2161 gccgggtgtg aaatatcgca agaaagtccc gcagtagtcc ggctgcccgc tgcgcgccgc
     2221 cgcatactcc ggggaaatga gcggcgagca atccccgcaa ttcgcctggc acgacacgcc
     2281 gacgcgcctg cgcacggcgc tgaagcagga cgagttcgtc ctgttctgcc agccgatcct
     2341 cgcgctgcgc ggcggcggcg gcttcgcgct cgccgaggtg ctcatccggc tgcgcgagga
     2401 ggagcaggcg ctgctgccgc cgggggag
//
LOCUS       DASYJA010000380         1767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1146700, whole genome shotgun sequence.
ACCESSION   DASYJA010000380 DASYJA010000000
VERSION     DASYJA010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146700"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VGO02_08385"
     CDS             complement(<1..505)
                     /locus_tag="VGO02_08385"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEV7477741.1"
                     /translation="MNLIEEAAKRLRQLEAAGVARTPHAPQEAPGTQPRLVPAEPGRR
                     PHEARPAPARTLDLARLQASGLITPALQHSKLLQEFRLVKRPLIKHALGRSAAQTPNR
                     NLIMVTSALPGEGKTFVAVNLAMSLAMEVDCRVLLVDADVLAPSVPEVLRIEPAKGLM
                     DVLTGAGT"
     gene            complement(510..>1767)
                     /locus_tag="VGO02_08390"
     CDS             complement(510..>1767)
                     /locus_tag="VGO02_08390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XrtA system polysaccharide chain length
                     determinant"
                     /protein_id="HEV7477742.1"
                     /translation="ARENLLDGLSRTVYIKSIGQNLYSIGFTHRDRAQATRVVQSLLS
                     IFVESGLAPKGKDAGQAQRFIEEQIKQYEQKLSEAENRLKEFKLKNIDLNVPQGRDFF
                     GTMATLTESLQEARLLLQEAEKSRDALKQQITDEEERPPVLLGALSENAALVVTPELD
                     ARIATLTKNLDDMLLRYTDGHPDVLNTRRIIREAETQREQERRRLAEEQERRRRAAGP
                     AAANPVYPQLKLAMAESEAQVARLRARVHEQEQRLEKLRLAARSVPEREAQLTQLNRD
                     YAIQKQNYDALVSRRESALMSGEVQAATGVASFRIVDPPQVSPHPVAPNRKMLVLLAL
                     LASLGAGLAASYFFSLLHPTFHDARGLKKVTHRPVLGTVSLVATPEVLWRRRRGTLAF
                     VGGLGGLGAAYFGVLALVFFRALLPF"
BASE COUNT          256 a          587 c          622 g          302 t
ORIGIN      
        1 tggtgccggc gccggtcagc acgtccatca ggcccttcgc cggctcgatt cgcagaacct
       61 cggggaccga gggtgcgagc acgtcggcgt cgaccagcag cactctgcag tcgacttcca
      121 tggccaggct catcgcgaga ttgaccgcca cgaaggtctt gccctcgccg ggcagggcac
      181 tggtcaccat gatgaggttg cggttggggg tctgcgccgc cgaccttccc agggcatgct
      241 tgatcagggg ccgcttcacc agccgaaact cctgcaacag cttggagtgc tggagcgcgg
      301 gggtgatcag cccggaggcc tgaagccggg ccaggtccag ggtgcgcgcc ggagcggggc
      361 gcgcctcgtg gggacgcctg ccgggttcgg ccgggaccag cctgggctgg gtcccgggcg
      421 cctcctgtgg cgcatgcggc gtgcgcgcca cgccggccgc ttcgagctga cgcagccgct
      481 tggctgcctc ttcgatgagg ttcataggct cagaacggca gcaaggcgcg gaagaaaacc
      541 agcgccagca cgccgaaata ggcagcgccg agcccgccca gcccgccgac aaaggcaagc
      601 gtgccgcgcc ggcgccgcca aaggacttcg ggcgtggcga ccagggacac ggtcccgagc
      661 accggccggt gggtgacttt cttgaggccg cgtgcgtcgt ggaacgtagg atgcagcagg
      721 ctgaagaaat agctggcagc caacccggcg ccgagcgacg caagcagcgc gaggagcacc
      781 agcatcttgc ggttcggcgc gaccggatga ggcgagacct ggggcggatc gacgatgcgg
      841 aagctcgcca cgccggtcgc cgcctggact tcgcccgaca tcagggccga ttcgcggcgg
      901 gagacgagcg cgtcgtagtt ctgcttctgg atcgcgtagt cgcggttgag ctgcgtaagc
      961 tgggcttccc tttcgggcac cgagcgcgcc gcgagccgca gcttttcgag gcgctgctcc
     1021 tgctcgtgca cccgcgcacg caggcgcgcg acctgcgcct cggactcggc catggcgagc
     1081 ttcagctgcg gatagaccgg attggctgcg gccggaccgg cggcgcgcct gcgccgctcc
     1141 tgttcttccg cgagccgcct gcgttcctgc tcgcgctgcg tttccgcttc cctgatgatg
     1201 cgccgcgtgt tcagcacgtc cggatgaccg tcggtatagc gcagcagcat gtcgtcgaga
     1261 ttcttcgtca gcgtggcgat gcgcgcgtcg agctccggcg tgacgacgag agcggcgttc
     1321 tcggaaagcg cgccgaggag caccggcggg cgctcctcct cgtcggtgat ctgctgcttc
     1381 agcgcatcgc gcgacttctc ggcctcctgc agcagcaggc gggcctcctg caggctttcg
     1441 gtcagcgtcg ccatcgtgcc gaagaagtcg cggccctgcg gcacgttcag gtcgatgttc
     1501 ttcagcttga attccttgag gcggttctcc gcctcggaga gcttctgctc gtactgcttg
     1561 atctgctcct cgatgaagcg ctgggcctgg ccggcgtcct tgcccttggg cgcgagcccg
     1621 gattccacga agatcgagag cagcgactgc accacgcgcg tggcctgggc ccggtcgcga
     1681 tgcgtgaagc cgatgctgta caggttctgc ccgatgctct tgatgtagac ggtgcggctc
     1741 agcccgtcga gcaggttctc ccgtgct
//
LOCUS       DASYJA010000381         7163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2417355, whole genome shotgun sequence.
ACCESSION   DASYJA010000381 DASYJA010000000
VERSION     DASYJA010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7163
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2417355"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="VGO02_08395"
     CDS             <1..1000
                     /locus_tag="VGO02_08395"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEV7477743.1"
                     /translation="LLAVAAARANGHKIDWQSYTPPAPAKPGITVLRDYPIAELVDYI
                     DWTPFFQAWELSGPYPKILQDPVVGDAARKVFAEAQEMLEKIVRGNWVTAHGVLGIFP
                     AAQAGGEDIEIYRDDARREQLMTWHNLRQQNQKPTGRANLCLADFVAPRESGVRDWAG
                     AFAVCAGGIEQRVADFEARHDDYNAIMLKVLADRLAEAFAERLHERVRREFWGYASDE
                     RLTSVQLIGEAYRGIRPAPGYPACPDHTAKGDLFRLLDAERNADMRLTESFAMLPTAA
                     VSGFYLSHPDSTYFAVGKIGRDQLEDFARRAGMSVADAQKWLAPYLDYETEAATTL"
     gene            1007..1108
                     /locus_tag="VGO02_08400"
     CDS             1007..1108
                     /locus_tag="VGO02_08400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HEV7477744.1"
                     /translation="MGKGDKRTRKGKIYKGSYGKKRSHRVKKTKKKS"
     gene            complement(1109..1294)
                     /locus_tag="VGO02_08405"
                     /pseudo
     CDS             complement(1109..1294)
                     /locus_tag="VGO02_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299529.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(1562..2029)
                     /locus_tag="VGO02_08410"
                     /pseudo
     CDS             complement(1562..2029)
                     /locus_tag="VGO02_08410"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2009..2356)
                     /locus_tag="VGO02_08415"
                     /pseudo
     CDS             complement(2009..2356)
                     /locus_tag="VGO02_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2361..4439)
                     /locus_tag="VGO02_08420"
     CDS             complement(2361..4439)
                     /locus_tag="VGO02_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV7477745.1"
                     /translation="MRLLLVIGGAVAATGVFLLATASSESARLGMHYPLLLMLNAALA
                     ALLAALVVYQLVVLVRRYRARVFGARLTLRLLGRFAVLAVVPGLIVYAVSVQFLTRSI
                     ESWFDVKVDAALEGGMNLGQQAIDQMMLEMQGKARTMAAELSERSAPQVVARLERLRA
                     QVGVEEALVVSASGRLVASATAEVTKLVPDLPSGQTLRQARTNRGYAAVDAVAGSPLS
                     LRVLIPLEARGIADEAQFLQVRSNVPASLARNAEAVEVAYRDYRELALSRDGLKRIYV
                     VTLTFALLMALFVAVAVAVTQSNLLAEPLANLAQATQAVARGDFSRRAPVTSRDELGV
                     LTESFNSMTRQLEEARRTAESSRVALEAAKGYLENILANLSAGVLVFDHEFVLSTSNH
                     GAQSILGPELDAFAGRMREYFLAHGEESWQLELALKGTAKTLHARGARLPRATGGGYV
                     VVIDDVTQLIHAQRATAWAEVARRLAHEIKNPLTPIQLSAERLEMKLSDRLSREDAET
                     LRRGTETIVNQVAALKSMVDDFRDYARLPAPAPKPLDLNALVRDVLVLYENSSVPIAL
                     QLSAGLPPVWADSAQMRQVIHNLVQNAQDALESAAATDGIKPAIEVRTELAGDRVRLA
                     VSDNGGGFPEELMARIFEPYVTTKPRGMGLGLAIVKKIVDEHHGSVAIENRPSRGASV
                     SVLLPLAKAA"
     gene            complement(4436..5029)
                     /locus_tag="VGO02_08425"
     CDS             complement(4436..5029)
                     /locus_tag="VGO02_08425"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEV7477746.1"
                     /translation="MRRIAHVTALLLAMAVFGASANEAEVRSASLEAVDDGLVLNADF
                     TFELSPRLEEVVANGVPLYFRVEFELTRPRWYWLDEKAASRVLQLRLSYHALSRHYRL
                     STGLLQQNFTTLNEALNVLKRVRNWLVVDRTATFSDADYEAAVRLRLDAGMLPRPFQL
                     SALTSRDLQLESAWKRFIVRPAQLPAPVESRNPQGAR"
     gene            complement(5040..5636)
                     /locus_tag="VGO02_08430"
     CDS             complement(5040..5636)
                     /locus_tag="VGO02_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477747.1"
                     /translation="MQDAAAQRAAPCLDLAPGLRVLDACAAPGGKAAHILETADVELT
                     AIDLDPARTERIGGNLERLGLAAKRIASADCRHPAQWWDGQPFDRILADVPCSASGVV
                     RRHPDIKWLRREQDVAAFAARQGAILDALWQVLGPGGKLLYVTCSVFPQENEAVAEAF
                     LRRAPHAARLELADGDPAQWLPGAEHDGFYYALIQKRA"
     gene            complement(6004..6273)
                     /locus_tag="VGO02_08435"
                     /pseudo
     CDS             complement(6004..6273)
                     /locus_tag="VGO02_08435"
                     /inference="COORDINATES: protein motif:HMM:NF013216.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
     gene            complement(6315..>7163)
                     /gene="fmt"
                     /locus_tag="VGO02_08440"
     CDS             complement(6315..>7163)
                     /gene="fmt"
                     /locus_tag="VGO02_08440"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEV7477748.1"
                     /translation="HDIALVLTQPDRPAGRGLRPLANAVKQLALARGLPLVQPERLQA
                     EDLERLRAVRADALVVAAYGLLLPPPALAVTTHGAWNIHASLLPRWRGAAPIQRALLA
                     GDRETGISIMQMEAGLDTGPVLAQQRLPISDDDDAGSLHDRLATLGAEMMVAALNEIE
                     AGRAHPTPQPGEGATYARKIEKREALLDWNHPAGELERTVRAFNPVPGAGTVLEGEPV
                     KVWRARLAAGKGQPGEVLEAGSDLVIACGEEALQILELQRSGAKRLEAAQFLRGRPLA
                     AGARFG"
BASE COUNT         1201 a         2460 c         2388 g         1114 t
ORIGIN      
        1 cttgctcgcg gttgccgccg cccgcgccaa cggccacaag atagattggc agagctacac
       61 gccgccggcg cccgccaaac cagggattac ggtcctcagg gattacccga tcgccgaact
      121 ggtcgactac atcgactgga cgccgttctt ccaggcctgg gagctttcag gcccttatcc
      181 caagattctc caggacccgg tagtcggcga tgcggcgcgc aaggtctttg cggaggctca
      241 ggagatgctc gagaagatcg tgcgcggcaa ctgggtcacc gcccacggcg tgctcggtat
      301 ctttccggcg gcgcaagccg gcggcgagga tattgaaatc taccgcgacg acgcgaggcg
      361 cgagcagctc atgacctggc ataacctacg ccagcagaac cagaagccca ccggccgcgc
      421 caacctgtgc ctcgcggact tcgtcgcgcc acgcgaaagc ggcgtgcggg actgggcagg
      481 tgcgttcgcc gtctgcgccg gtggcatcga gcaacgcgtg gccgacttcg aggcacggca
      541 cgacgactac aacgccatca tgctgaaggt gctcgccgac cgcctggccg aggccttcgc
      601 cgagcggctg catgagcgtg taaggcgcga gttctggggc tatgcctcgg acgagaggct
      661 gacgagcgtt cagctgatcg gcgaagccta tcgcggcatc cggcctgcgc cgggctaccc
      721 cgcctgtccc gaccacaccg caaaaggcga cctcttccgc ctgttagacg ccgagcgcaa
      781 cgccgacatg cgactcacag agtccttcgc catgctgccc actgctgcgg tgtcgggctt
      841 ctacctgtcg catcccgact cgacctactt cgcggtcggc aagatcgggc gcgaccagct
      901 cgaggatttc gcacgccgcg cgggaatgag cgtcgccgac gcgcaaaaat ggcttgctcc
      961 ctaccttgac tacgagaccg aggcggcgac aacgctataa tcagccatgg gtaaaggcga
     1021 caagcgcacg cgcaagggca agatctacaa gggcagctac ggcaagaagc gctcgcaccg
     1081 cgtcaaaaaa acgaagaaaa agagctagtc agctctcctc gcggcggccc accggcagtc
     1141 cgagcgcctt caatttgcgg tagaggtgcg tgcgctcgag gcccgagcgc tcggcaacgc
     1201 gcgacatgct gccatgctca cgcgctagga gctgctcgaa gtagacccgc tcgaacgcct
     1261 cccgcgcctc gcggtagggg cgatcgaaag cgacctcggg aagcgccccc tgcgagcggc
     1321 gagcggcgag cgagcgctcg acgtcctcga gctggatttc ctcctctagc gcagtaagcg
     1381 cgagatcctt gaccacgctt tccagctccg cgagatttcc cggccaggcg tggtggcgca
     1441 gcgcatttag ggccgcgatc gccaggcgcc gctgcgggca gagccgcgcc gcgaccagct
     1501 gagccagcat gagcgaggcg aggtcgggga tgtcctcggc gaattcgcgc agcgcgggca
     1561 gcctgaaggt cagctcgccg agccgtgcac cgagttcggc gtcgaactcg tgcttctcgg
     1621 cgagcgtgcg catgtcgagc ggagaaaacg acaccacgcg caccttgtgc ttctcggtac
     1681 gcgccagcat gaaatccaga ttgcgttgct cgacgcgacc cagccgcgac aggtcgggca
     1741 cgaagagcac gcctccggcc gccttcgtga gcagctcggt cggtggctcg gcgagctgct
     1801 cggcgcccga gaggaagggc gcgccctggg cggcgagcag gcgcgcgaac atctccgggt
     1861 gcatgccgcg ctcaccgcgc agcagcagcg gcgcgccgga ctgggcgagc tgtgccaagc
     1921 gcttcttggc gtcggcgagc gcggcagagc gaccaagggc ggccagcgcg aggcgcggcg
     1981 cgaccgccgc ctcgggatgg cgcagcgcgc gcttgacggt cgagagcagg cgctgcagcg
     2041 caatcggctt ctccagaaaa tcgagtgcgc cgatgcgtgt ggcttcaacc gccgtttcga
     2101 tggtggcgtg gccggacatc atgatcaccg gcatggtgag ctggccgttc gccgcccact
     2161 ccttcagtag cgtgatgccg tccgtgtcgg gcatccagat gtcgagcagc accaggtcgg
     2221 gccgtgctcg ctcgcgcagc cggcgcgcat caccggcgct ctccgccagc gccacctggt
     2281 gaccctcgtc ggcaagaatc tccgagagca gctcgcggat gcccacctcg tcgtcgacta
     2341 caaggatctg agccatggcg ctatgccgcc ttcgcgagcg gcagcagcac gctcaccgag
     2401 gcgcctcggc tcggccggtt ctcgatcgcc acgctgccgt gatgctcgtc gacgatcttt
     2461 ttcacgatgg cgagcccgag gcccataccg cgcggtttgg tggtcacgta gggctcgaag
     2521 atacgcgcca tcagctcctc cggaaaaccg ccgccgttgt ccgacacggc gaggcgcacc
     2581 cggtcgccgg cgagctcggt gcgcacttcg atcgcgggct tgatgccgtc ggtggccgcg
     2641 gcggactcga gggcgtcctg cgcgttctgc accaggttgt ggatcacctg gcgcatctgc
     2701 gcgctgtcgg cccagaccgg cggcaggccg gcgctcagct gcaaggcgat cggcacgctg
     2761 gaattctcgt acagcacgag cacgtcgcgc accagagcgt tcaggtcaag gggcttgggc
     2821 gcaggcgcgg gcaggcgtgc gtagtccctg aaatcgtcca ccatcgactt cagggccgcg
     2881 acctggttga cgatggtctc cgtaccccgc cgcagcgttt ccgcgtcctc gcgcgacagg
     2941 cgatcggaca gcttcatctc cagccgctcg gccgaaagct gaataggtgt gagcggattc
     3001 ttgatttcgt gcgcgaggcg ccgggcgact tctgcccagg cggtggcgcg ttgcgcgtgg
     3061 ataagctgcg ttacatcgtc gatcaccacc acgtaaccgc cgcccgtagc gcgcggcagc
     3121 cgggcgccgc gcgcgtgcag cgtcttggcc gtgcccttca gcgccagctc gagctgccag
     3181 ctctcctcgc cgtgcgcgag gaaatattcg cgcatccggc cggcgaaagc gtcgagctcg
     3241 ggacccagta tcgattgcgc gccgtggttg gacgtcgaca gcacaaactc gtggtcgaac
     3301 accagcacgc cggccgacaa gttggccagg atgttctcga ggtatccctt ggcggcctcc
     3361 agcgccacgc ggctcgactc ggcggtgcgc cgcgcctcct ccagctgccg ggtcatcgag
     3421 ttgaacgatt cggtgagcac accgagctcg tcgcggctgg tgacgggcgc gcgccgggaa
     3481 aaatcgcccc gcgccacggc ctgcgttgcc tgcgcgaggt tggcaagcgg ttctgcgaga
     3541 aggttggatt gggtcaccgc caccgccacc gcaacgaaca gcgccatcag cagggcgaag
     3601 gtcagcgtca ccacgtagat cctcttcagg ccgtcacgtg aaagggcgag ttcgcgatag
     3661 tcgcggtacg ccacctcgac cgcctccgcg ttgcgcgcga gcgaggccgg tacattggag
     3721 cgcacctgca ggaattgcgc ctcgtcggcg atgccgcgcg cttcgagcgg gatcagaacg
     3781 cgcagcgaaa gcggacttcc cgcgaccgcg tccactgcgg cataaccgcg gttggtgcgt
     3841 gcctgacgaa gcgtttggcc actcggcagg tcgggcacca gcttggtcac ctctgcggtc
     3901 gcgctcgcca ccaggcggcc gcttgcgctc accacgaggg cctcctccac cccgacctgg
     3961 gcgcgcagcc gttcgaggcg tgcaacgacc tgcggcgcag accgctcgct cagctcggcc
     4021 gccatggtgc gcgccttgcc ttgcatctcg agcatcatct ggtcgatcgc ctgctggccg
     4081 agattcatgc cgccttcgag cgccgcgtcg accttgacgt cgaaccacga ctcgatcgag
     4141 cgcgtcagga attgcaccga gaccgcgtag acgatcaagc ctggcaccac ggcaagcacg
     4201 gcgaagcgtc cgagcagccg cagcgttagg cgcgcgccga agacacgcgc ccgatagcgc
     4261 cgcaccagga caacgagctg gtagacgacg agcgcggcga gcagcgcggc gagcgctgca
     4321 ttcaacatca gcagcagcgg atagtgcatg ccgaggcgcg cgctttccga gcttgccgtg
     4381 gcgagcagga acacccctgt cgcagccact gcgccgccga tcacgaggag cagcctcacc
     4441 ttgccccctg cggattgcgg ctctcgaccg gagccggcaa ctgggcgggc cgtacaatga
     4501 atcgcttcca cgccgactcg agctgcaggt cccggctggt aagcgcgctc agctgaaaag
     4561 gcctgggcaa catgccggcg tcgagccgca ggcgcaccgc ggcttcgtag tccgcgtccg
     4621 aaaacgtggc ggtgcggtca acgaccagcc aattgcgcac gcgcttgagc acattcaagg
     4681 cttcgttgag agtggtgaaa ttctgctgca acaggcccgt ggacagccga tagtgccgcg
     4741 acagcgcatg ataggacagc ctgagctgca gcacgcggga agcggccttt tcgtccagcc
     4801 agtaccagcg cggccgcgtc agttcgaact cgacgcggaa atagagcgga acgccgttgg
     4861 caacgacctc ctcgaggcgc gggctgagct cgaacgtgaa atccgcgttg agcaccagcc
     4921 cgtcgtcgac cgcctcgagg ctcgccgagc gcacctcggc ttcgttcgcg ctcgcgccaa
     4981 agacggccat ggcgaggagc agcgcggtga catgagcgat gcggcgcatc gcgcgtccgt
     5041 caggctcgtt tttggatcaa ggcgtagtag aagccatcgt gctcggcgcc gggcaaccac
     5101 tgcgccgggt cgccgtcggc gagctcgagc cgcgctgcgt gcggcgcgcg acggaggaaa
     5161 gcctcggcca cggcttcgtt ttcctgcgga aacaccgagc aggtgacata cagcaattta
     5221 ccacccggcc ccagcacctg ccacagcgcg tcgaggatcg caccctgccg cgcggcgaac
     5281 gccgcaacgt cttgctcccg gcgcagccat ttgatgtcgg ggtggcgtcg taccaccccg
     5341 gatgccgagc aaggaacatc ggccaggatg cggtcgaacg gctggccgtc ccaccattgc
     5401 gcggggtggc ggcagtcggc gctcgcgatc ctcttcgcag cgaggccgag gcgctcgaga
     5461 ttgccgccga tacgctcggt gcgcgccgga tcgaggtcga tcgctgtcag ctcgacgtcg
     5521 gccgtctcca ggatgtgcgc ggccttgcca ccgggcgcgg cgcacgcgtc gagtacgcgt
     5581 agccccggcg caaggtccag gcatggggcg gcgcgctgcg ctgctgcgtc ctgcacagac
     5641 acctcgcctg ccgcaaaaac ccggcaggcg ctccgcaggc atgggccgct caagcacgag
     5701 agcgtctccc acgacacggc gcgccacgat tccctcggcc gcgagcctcg cttcgtaccc
     5761 ttcggcagat atgcgtctgc ggttcacgcg cagcgtcatc ggcggatgcg tgttgccagc
     5821 cgccagtact gttccccact caccgggcca ggcggcacgc accgcgtcta tccaccagcg
     5881 cggatgctgg tgcaatgcct ccggatcgcc agccagtcgt gtctccagtg tcgcgcggct
     5941 gcgcagagcc ccgcgcagca ctgcgtttac gtaacccttt gcattccatt tctgcagcag
     6001 ccggcaggcc cgcactgcct gatcgacgac ggtatattcg gcgtagcgtc ccgattcgag
     6061 cgcgtagagc gagcaccaga gcagggcgtc gagcagagga tcggagcttt cgcggcgtga
     6121 caattcgctc acgatcttct gcacccgtcc gtagcgccgc agcgtgccgt gcgtgagatc
     6181 gagcaacgcg gcgcgcggcg tggcgctgcc ttcctcggca atgcgctcga attctcccgc
     6241 aaggctggcg ccgccagcga cacgggcaac catctgggct gcgtcgccca gtgcgcccgc
     6301 cagcgcgtgc gcgctcaccc gaagcgagcg cctgcggcga gcggccgccc gcgcaggaac
     6361 tgtgcagcct cgaggcgctt cgcgccagag cgctgcaact cgaggatctg cagcgcttcc
     6421 tcgccgcagg cgatcaccag atcagagccg gcttccagga cctcgccggg ctgtcccttt
     6481 cccgcggcga gacgggcgcg ccacaccttc accggttccc cctccagaac ggttcctgca
     6541 cccggcacag ggttgaaggc gcgcacggtc cgctcgagtt cgcccgcggg atgattccag
     6601 tcgagcaatg cctcgcgttt ttcgatcttg cgcgcgtagg tggcgccttc tcccggctgc
     6661 ggtgtgggat gtgctcggcc ggcttcgatt tcgttcagtg cggcaaccat catttcggcg
     6721 ccgagcgtgg caagcctgtc gtggagcgat cccgcatcgt cgtcatcgga gatcggcagg
     6781 cgttgctgcg cgaggaccgg gccggtatcg agcccggctt ccatctgcat gatggaaatg
     6841 ccggtctcac gatcccctgc gaggagcgca cgctggatcg gcgcagcgcc gcgccagcgc
     6901 ggcagcaggg aagcgtggat gttccaggcg ccatgcgtgg tcaccgccag cgcgggtggc
     6961 ggcagcaata gaccgtacgc ggcaactacc aaggcgtcgg cgcgcaccgc tctgagacgc
     7021 tcgagatcct cggcctgcag acgctccggc tggaccagcg gcagaccgcg cgccagggca
     7081 agctgtttca cggcattggc cagtggtcgc aagccgcgcc cggcgggtcg atcgggctgc
     7141 gtcagaacga gcgcgatgtc gtg
//
LOCUS       DASYJA010000382         2492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1057016, whole genome shotgun sequence.
ACCESSION   DASYJA010000382 DASYJA010000000
VERSION     DASYJA010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057016"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="VGO02_08445"
     CDS             complement(<1..901)
                     /locus_tag="VGO02_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7477749.1"
                     /translation="MATLTETSARASGMTKDEKFVIFASSLGTVFEWYDFYLYATLAP
                     FFAALFFPSGNDTAALLSAFATYAAGFLVRPFGALIFGRIGDLVGRKYTFLVTIVVMG
                     TATFLVGLLPTFSQIGWLAPVLLVTLRLCQGLALGGEYGGAATYVAEHSRPHERGYTT
                     SFIQTTATLGFFLALVVIGLCRIYMDAKMFADWGWRIPFLVSLILLVFSVYIRLRLSE
                     SPIFMRMKEEGKGSKAPLTDSFLRYPNNRLVLLALLGATAGQGVVWYTGQFYALFFLL
                     IYLKLDFLKTYQLVGLSLLIGTPF"
     gene            complement(1262..2230)
                     /locus_tag="VGO02_08450"
     CDS             complement(1262..2230)
                     /locus_tag="VGO02_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV7477750.1"
                     /translation="MTAVATPVFALEAARPSQGCRAERPPGLILRRRHGSVAPTAGIS
                     MRPRGPRTPESYQPRKPGQPGKTGWKRTPKRGRSPRGAETRAGDGAVILYGWHTVKAA
                     LENPARRIRRLFATENAARRLAEDGLAPGVETELVRPDAIAHRLGPDAVHNGLLAEAD
                     PLPSPELDELEPAGIVLVLDQITDPHNVGAILRTAAGFAVAAVVTTARHSPEATGVLA
                     KSASGALEYVPIVSVQNLARALELLRERGYLLVGLDSTGDIDLGDAPLRAPLALVLGA
                     EGKGLRQLTRATCDRVARLDLPGRIKSLNVSNAAALALYIASKRAS"
     gene            2341..2426
                     /locus_tag="VGO02_08455"
     tRNA            2341..2426
                     /locus_tag="VGO02_08455"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2375..2377,aa:Tyr,seq:gta)
BASE COUNT          430 a          774 c          871 g          417 t
ORIGIN      
        1 agaagggggt gccgatcagc aacgagagac cgaccaactg gtaggtcttg aggaagtcga
       61 gcttcaggta gatcagcagg aagaagagcg cgtagaactg gccggtgtac cagaccacgc
      121 cctggccggc ggtcgccccc agcagggcca gcaggaccaa ccggttgttg gggtagcgca
      181 ggaagctgtc ggtcaggggc gccttggagc ctttgccctc ttccttcatg cgcatgaaga
      241 tcggggactc gctcagcctg agccggatat agaccgagaa caccagcagg atcagcgaca
      301 ccaggaatgg aatgcgccag ccccagtcgg cgaacatctt tgcgtccatg tagatccggc
      361 agaggccgat cacgacgagg gcgaggaaga agccgagcgt ggccgtggtc tggatgaagc
      421 tcgtggtgta gccgcgttcg tgcgggcgcg aatgctcggc gacataggtt gcggcgccgc
      481 cgtattcgcc gccgagggcg aggccctggc acagacgcaa cgtcaccagg aggacaggcg
      541 ccagccagcc gatctgggag aaggtcggca gcagccccac gaggaacgtc gcagtaccca
      601 tcacgacgat ggtcacgagg aaggtgtatt tgcgtccaac cagatcgccg atgcggccga
      661 agatcagcgc gccgaacggg cgcaccagaa agcccgccgc gtaggtagcg aaggctgaca
      721 gcagtgcggc cgtgtcgttg ccggacggga agaataacgc ggcgaagaac ggcgcgagcg
      781 tcgcatagag atagaagtcg taccattcga acaccgtgcc gagcgatgat gcgaagatga
      841 cgaacttctc gtccttcgtc atgccgctcg cgcgggctga ggtttctgtt agcgttgcca
      901 taggcgtttt ccctgactgg ttctgctgcc tgggaggcca atgaacggct gccgaggcgc
      961 cccacttgct gcgacgcttc cgatctgggg tcgggtggcg aagcggcagg aaggtaccat
     1021 tggcgtgtcg ggcgcccgta taagactttc ggcctaggaa acggcctcta cgtgttgaaa
     1081 acattgcgat gacggtacct ggccagctgc cacctcgggt tgcgggttat tgcgaagcgg
     1141 cggccgtggg tctcatctgg ggtttctcgc gcgtccacgc gtccgcgata tgtctgacgc
     1201 ggccggcatt attgacatta ttgacattga cgggggagga ccgctgctgc gcggtcgagc
     1261 atcagcttgc gcgcttgctg gcaatataaa gcgccagcgc cgcggcgttc gagacgttga
     1321 ggctcttgat ccgaccgggg agatcgagcc gcgccactcg gtcgcacgtg gcgcgggtca
     1381 attgccgcag tcccttgccc tccgcgccga gaaccagcgc gagcggggcg cgcagcggcg
     1441 cgtcgccgag gtcgatgtct cccgtgctgt cgaggccgac caggaggtat ccgcgctcgc
     1501 gcagcagctc gagcgcgcgg gcgagattct gcaccgagac gatgggcaca tattcgagcg
     1561 cgccggaggc ggattttgcc agcactccgg tcgcttccgg actgtgccgc gcggtggtga
     1621 ccaccgccgc caccgcgaag cccgccgcgg tgcgcagaat ggcgccgaca ttgtgcggat
     1681 cggtgatctg gtcgagcacg agcacgatgc cggcgggctc gagctcgtcg agctcgggcg
     1741 agggcagggg atcggcttcg gccaacagtc cgttgtgcac ggcatcaggt ccgagccggt
     1801 gcgcgatcgc gtccgggcgc acgagctcgg tctcgacgcc cggcgccagg ccgtcctcgg
     1861 ccaggcggcg cgccgcgttc tcggtggcga acagacgccg gatccgccgt gccggattct
     1921 ccagcgccgc cttcaccgtg tgccagccgt agaggatcac cgcgccgtcg cccgcccggg
     1981 tctcggcgcc acgcggtgag cgaccgcgct tcggggtgcg cttccagccg gttttgcccg
     2041 gctgacccgg cttgcgcggc tggtaggact cgggcgtgcg tgggccgcgt gggcgcatgg
     2101 atattcctgc tgtcggcgcc acactcccgt gccgccggcg caagatcaag ccgggcgggc
     2161 gctcggcgcg gcacccttgc gacgggcgcg cggcttcgag cgcaaaaacc ggcgtggcca
     2221 cggccgtcat gggcgacccg ccggagccgt tttcgcgtgg ttgacaccgc ccaaggcgct
     2281 ccccataacc ccgccctgct cacgggaacg cgcatccgca gggatgcgac gttcgacggc
     2341 gggggagtgt cccgagtggc aaagggggcg gactgtaaat ccgctggctt acgccttcgt
     2401 aggttcgagt cctacctctc ccaccatctt gttttcattg aataatcttg aaataggccg
     2461 ggccaaaatc cggctgtttt catgttgcgc tt
//
LOCUS       DASYJA010000383         1875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_329053, whole genome shotgun sequence.
ACCESSION   DASYJA010000383 DASYJA010000000
VERSION     DASYJA010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329053"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VGO02_08460"
     CDS             complement(<1..399)
                     /locus_tag="VGO02_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7477751.1"
                     /translation="MLLELTGVRAGYGDAIVLDDVSLSLPENGSLAVLGRNGVGKSTL
                     LLTVMGMTRVSRGEILFAGRSITSLPPHRRARLGLGWVAQEREIFPSLTVEENLTVAA
                     RPGPWSLSSINQLFPRLQERKGNLGNQLSGG"
     gene            complement(389..1090)
                     /locus_tag="VGO02_08465"
     CDS             complement(389..1090)
                     /locus_tag="VGO02_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7477752.1"
                     /translation="MVAQDIDLALPKGVRYALIGPNGAGKTTLINLMTGMLRPDSGQI
                     FLGEDDITALPPDKRVHRGLARTFQINTLFPQLTPLESVALAVFERQGISRVWWRNLP
                     SYRDAIEEAHGILTSLRLHEVANRQTKELAYGQQRLLEIALALATKPRVLLLDEPAAG
                     VPKDESAELFEAIASLSAELTILFIEHDMGVVFRFASRILVMVGGRILVEGTPHEISR
                     DERVREVYLGRRHAA"
     gene            complement(1107..>1875)
                     /locus_tag="VGO02_08470"
     CDS             complement(1107..>1875)
                     /locus_tag="VGO02_08470"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV7477753.1"
                     /translation="HAAFFGVGAYTAGLIAKHYWTEPISGLFLAAIFSGLLGYALSHI
                     IVRFRHLALIMLTLGFGLLLHEAANSASMLTGGADGLQGFRVPGVLNFQFDLYGKTAY
                     AYCLAVLFICFLFSRRIIHSPFGLALRGIRENATRMPAIGAASQSHLRKAYTLSAVLA
                     GIAGALLTHTTSTVSLETLSFGRSADVLVILILGGAGRLYGGILGAIIYMVARDQFSG
                     VAPQFWYFWIGLLLVAVVMFLPNGILGGLSALHKKNQ"
BASE COUNT          358 a          586 c          635 g          296 t
ORIGIN      
        1 gccgccagag agctggttgc cgaggttgcc tttcctctct tgcagccgcg gaaaaagctg
       61 gttgatggaa gaaaggctcc acggccccgg acgcgcggcc accgtcaggt tctcctccac
      121 cgtgagcgag gggaagatct cgcgctcctg cgcgacccag cccaggccga ggcgcgcccg
      181 ccggtgaggc ggcagggagg tgatgcttct tcctgcaaaa aggatctcac cccgcgacac
      241 ccgggtcatg cccatcacgg tgagcagcag ggtcgacttg ccgacgccgt tgcggccgag
      301 gaccgcgagg ctgccgttct cgggcaggga aagggaaacg tcgtccagca cgatcgcgtc
      361 gccgtagccg gcgcgcacgc ctgtcagctc aagcagcatg gcgcctgccg aggtagacct
      421 cgcgcacgcg ctcgtcgcgc gagatctcgt gcggcgtgcc ttcgaccagg atgcggccgc
      481 cgaccatgac caggatgcgg ctggcgaagc ggaacaccac ccccatgtcg tgctcgatga
      541 acaggatcgt gagctccgcg gagaggctgg cgatcgcctc gaacagctcc gcgctctcgt
      601 ccttcggcac gccggccgcc ggctcgtcga gcagcagcac gcgcggcttg gtggcgaggg
      661 cgagcgcgat ttcgagcagg cgctgctggc cgtacgcgag ctcctttgtt tgccggttgg
      721 cgacctcgtg cagccgcagg gaggtcagga tgccgtgcgc ctcttcgatg gcgtcgcggt
      781 agctcggcag gttcctccac cacacccttg aaattccttg cctctcaaaa acggcaaggg
      841 cgaccgactc gagcggcgtc agctgtggga acagcgtgtt gatctggaag gtgcgcgcaa
      901 ggccgcggtg tacccgcttg tcggggggta gcgcggtgat gtcatcctcg ccgaggaaga
      961 tctgccccga gtccgggcgc agcatgccgg tcatcaggtt gatcagcgtg gtcttgccgg
     1021 cgccgttcgg gccgatcagc gcgtagcgca cgcccttggg cagcgcgaga tcgatgtcct
     1081 gcgctaccac cagcgaaccg aatttcttac tgattttttt tgtggagagc acttaagccg
     1141 ccgagaatgc cattgggcag gaacatcacg actgccacca gcagcagccc gatccagaag
     1201 taccagaact gcggcgcgac gcccgagaac tggtcgcgcg ccaccatgta gatgatcgcg
     1261 ccgaggatgc cgccgtacag ccggccggcg ccgccgagga tcaggatcac cagcacgtcg
     1321 gccgagcggc cgaaggacag cgtctcgagc gagaccgtcg aggtggtatg cgtcagcagc
     1381 gctccggcga ttcccgccag caccgcggaa agggtgtatg ccttgcgcag gtgcgactgg
     1441 ctggcggcgc cgatcgccgg catgcgcgtc gcgttttccc tgatacctct gagggccaga
     1501 ccgaaagggg agtgaataat acggcgtgaa aataaaaagc aaataaacag gactgcaagg
     1561 caatacgcat aggcggtctt gccgtagaga tcaaactgga aattaagcac cccgggcacg
     1621 cgaaaaccct gcaaaccgtc ggcgccgccg gtgagcatgc tcgcgctgtt ggccgcctcg
     1681 tgcagcagca ggccgaagcc cagggtcagc atgatgagcg cgaggtggcg gaagcgcacg
     1741 atgatgtggc tcagcgcata cccgaggagg cctgaaaaaa tggctgccag gaaaagaccg
     1801 ctgatcggtt cggtccagta gtgcttggcg atcaggccgg cggtataggc gccgacgccg
     1861 aagaacgcgg catgg
//
LOCUS       DASYJA010000384         3345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1510077, whole genome shotgun sequence.
ACCESSION   DASYJA010000384 DASYJA010000000
VERSION     DASYJA010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510077"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VGO02_08475"
     CDS             <1..212
                     /locus_tag="VGO02_08475"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEV7477754.1"
                     /translation="QPGASRSEFAGEHGGRIKVRLAARAVDGKANQALIEFLSEHFKV
                     PKRNVRIESGLKSRRKRVVIEGINP"
     gene            complement(202..1974)
                     /gene="aceK"
                     /locus_tag="VGO02_08480"
     CDS             complement(202..1974)
                     /gene="aceK"
                     /locus_tag="VGO02_08480"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HEV7477755.1"
                     /translation="MAAVQPVARIQDRRGGLPLEVARAILAGFDKHYGLFRQAAVRAK
                     TLFEGAAWPEMRALARERIQMYDRRVEEAVRGLLDRFPQAEIDESVWPAIKLAYLGLL
                     HEHKQPECAETFYNSVACEVLHRRHYHNEFIFWRPGVATEHLEGEAPTYRGYYPLKDG
                     LRKTLKAIAGSFGLANRWEDPRRDLRNVVHALRGHFPRPARAQPDLQIQVLGSLFFRN
                     KAAYVVGRIINAHRDLPFAVPILQNRRGELFLDALLVGEDQLLVLFSFARAYFFVDME
                     VPAGYISFLRWLMPKKPRAELYMSVGLAKQGKTLFYRDLHYHLKHSTDRLVIAPGIPG
                     LVMLVFTLPSFPFVFKVIRDRFAPPKEIDRQTVKDKYLLVKLHDRVGRMADTLEYSRV
                     ALPLARFEPALVEELRRECASVLEFDGDDLVLGHVYIERRMQPLNLHIDELKRDGDEA
                     RLRVALREYGNAIKELAGAGIFPGDMLLKNFGVTRHERVVFYDYDEIQPMEEVVFRRI
                     PPAKSYEEETAAEPYWAIGANDVFPEQFEPFLVSDPRSKQMFYEDHRDLLDAAFWAGK
                     QERVRAGMQEDVFPYPQEIRFARG"
     gene            complement(1976..3304)
                     /gene="aceA"
                     /locus_tag="VGO02_08485"
     CDS             complement(1976..3304)
                     /gene="aceA"
                     /locus_tag="VGO02_08485"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEV7477756.1"
                     /translation="MSDRQKEIQTLEQDWAKNPRWHGVKRGYTAADVVRLRGSLKIEH
                     TLARHGAEKLWQLCHERPFVNSLGALTGNQAMQQVRAGVPAIYLSGWQVAADANDSLS
                     MYPDQSLYSTTSVPSVVRRINNALMRQDQIQTMEGKGDIDWFAPIVADAESGFGGVLN
                     AFELMKAMIDAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLIAARLAADVMGV
                     PTVLLARTDAEAADIVTSDIDENDKPFITGERTSEGFYKTRKGFDQALSRGLAYAEYA
                     DMVWCETGTPDLAFARKFAEGIRKKYPGKMLAYNCSPSFNWKRNLDDATIGKFQRELG
                     AMGYKFQFITLAGFHSLNYSMFELAYGYARNNMSAFVELQEKEFAAAEKGFTAVKHQR
                     EVGTSYFDQITQVVTGGKASTTALHGSTEDEQFFGEDALSQSKAKAKRAA"
BASE COUNT          531 a         1082 c         1137 g          595 t
ORIGIN      
        1 tccagccagg cgcatcacgc tccgaattcg ccggcgagca tggcgggcgc atcaaggtga
       61 gactggcggc acgcgcggtc gacggcaagg cgaaccaagc cctgatcgaa tttctgtccg
      121 agcatttcaa ggtcccgaag cgcaacgtgc gcatcgaatc ggggctgaag tcgaggcgga
      181 agcgcgtggt gatcgagggg atcaacccct agcgaaacgg atttcctgcg ggtaggggaa
      241 cacgtcctcc tgcatcccgg cgcgcacgcg ttcctgcttg ccggcccaga acgcggcgtc
      301 gagcaggtcg cggtggtcct cgtagaacat ctgcttcgag cgcgggtcgc tcaccaggaa
      361 aggctcgaac tgctcgggga acacgtcgtt ggcgccgatc gcccagtacg gctcggcggc
      421 ggtttcctcc tcgtagctct tggccggcgg gatgcgccgg aacacgacct cttccatcgg
      481 ctggatctcg tcgtagtcgt agaacaccac gcgctcgtgt cgcgtgacgc cgaaattctt
      541 gagcagcatg tcgccgggga agatgccggc gccggcgagc tccttgatgg cgttgccgta
      601 ctcgcgcagc gccacgcgca gccgcgcttc gtcgccgtcg cgcttcagct cgtcgatgtg
      661 caggttgagc ggctgcatgc gccgctcgat gtagacgtgg ccgagcacca gatcatcgcc
      721 gtcgaattcc aggaccgagg cgcattcgcg cctgagctcc tcgaccagcg ccggctcgaa
      781 gcgagcgagc ggcagcgcga cgcgcgagta ctcgagggtg tcggccatcc gcccgacgcg
      841 atcgtgcagc ttcaccagca ggtacttgtc cttcaccgtc tggcggtcga tctccttggg
      901 cggcgcgaag cggtcgcgga tcaccttgaa cacgaagggg aacgagggca gggtgaacac
      961 cagcatcacc agccccggga tccccggggc gatgaccaag cggtcggtcg agtgcttcag
     1021 gtggtagtgc aggtcgcggt agaagagggt cttgccctgc ttcgccaggc cgaccgacat
     1081 gtagagctcg gcgcgcggct tcttcggcat cagccagcgc aggaacgaga tgtagcccgc
     1141 cggcacttcc atgtcgacga agaaataggc gcgggcgaac gagaacagca ccagcagctg
     1201 gtcctcgccg accagcagcg cgtccaggaa cagctcgccg cggcggttct gcaggatcgg
     1261 cacggcgaaa ggcaggtcgc ggtgcgcgtt gatgatgcgc ccgacgacgt aggcggcctt
     1321 gttgcggaag aagagcgagc cgagcacctg gatctgcagg tcgggctggg cgcgggccgg
     1381 gcgcgggaag tggccgcgca gggcgtgcac gacgttgcgc aggtcgcgcc gcggatcttc
     1441 ccagcggttg gcgaggccga agctgccggc aatggccttg agcgttttcc tcaggccgtc
     1501 cttcagcggg tagtagccgc gataggtcgg cgcctcgccc tcgaggtgct cggtggcgac
     1561 cccgggccgc cagaagatga actcgttgtg gtagtggcgc cggtgcagca cctcgcaggc
     1621 gaccgagttg tagaaggttt cggcgcattc gggctgcttg tgctcgtgca gcaggccgag
     1681 ataggcgagc ttgatcgccg gccatacgga ttcgtcgatc tcggcctgcg ggaagcggtc
     1741 gagcagcccg cgcaccgctt cctccacccg ccggtcgtac atctggatgc gctcgcgcgc
     1801 gagcgcgcgc atctccggcc aggcggcgcc ttcgaacagc gtcttggccc gcaccgcggc
     1861 ctgccggaac aggccgtagt gcttgtcgaa cccggcgagg atggcgcgcg ccacctccag
     1921 cggcaggcca ccgcgccggt cctggatcct cgctacgggc tggactgctg ccacttcacg
     1981 ctgcgcgctt tgcctttgcc ttcgactgcg acagcgcgtc ctcgccgaag aactgctcgt
     2041 cctcggtcga accgtgcagc gcggtggtgg aggccttgcc gccggtcacc acctgcgtga
     2101 tctggtcgaa gtagctggtg ccgacctcgc gctggtgctt gaccgcggtg aagcccttct
     2161 cggccgcggc gaactccttt tcctgcagct cgacgaatgc gctcatgttg ttccgcgcgt
     2221 agccgtaggc gagctcgaac atcgagtagt tgagcgaatg gaagccggcc agcgtgatga
     2281 actggaactt gtagcccatc gcgccgagct cgcgctggaa cttgccgatg gtggcgtcgt
     2341 ccaggttgcg cttccagttg aacgacggcg agcagttgta ggcgagcatc ttgcccgggt
     2401 acttcttgcg gatgccttcc gcgaacttcc tggcgaaggc gaggtccggc gtgccggtct
     2461 cgcaccagac catgtcggca tactcggcgt aggccaggcc gcgcgacagc gcctggtcga
     2521 accccttcct cgtcttgtag aacccctcgg aggtgcgctc gccggtgatg aacggcttgt
     2581 cgttctcgtc gatgtcgctg gttacgatat ccgccgcttc ggcgtcggtt cgcgcgagca
     2641 gcacggtagg cacgcccatc acgtcggccg cgaggcgcgc cgcgatcagc ttctgcaccg
     2701 cctcctgggt cggcaccagc accttgccgc ccatatggcc gcacttcttc accgaggcga
     2761 gctggtcctc gaagtgcacg ccggcggcgc cggcgtcgat catcgccttc atcagctcga
     2821 aggcgttcag cacgccgccg aagcccgact cggcgtcggc cacgatcggg gcaaaccagt
     2881 cgatatcccc tttgccttcc atcgtctgga tctggtcctg gcgcatgagc gcgttgttga
     2941 tgcgcctcac caccgacgga acggaggtgg ttgaatagag cgactggtcg gggtacatcg
     3001 agagggaatc attggcgtcg gcggcgacct gccagccgga gagatagatc gccggcacgc
     3061 cggccctcac ctgttgcatc gcctgattgc cggtgagcgc gccgagcgaa ttcacgaacg
     3121 gccgctcgtg gcagagctgc cacagcttct ccgcgccgtg gcgcgcgaga gtgtgctcga
     3181 tcttcaggct gccgcgcagg cgaaccacgt cggcggcggt gtagccacgc ttgacgccgt
     3241 gccagcgcgg gttcttcgcc cagtcctgct cgagggtttg aatctctttt tgtcggtcgc
     3301 tcacgggtgt ctccggggtg aaatgaagtg gttgatgatc ccccg
//
LOCUS       DASYJA010000385         2758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_602353, whole genome shotgun sequence.
ACCESSION   DASYJA010000385 DASYJA010000000
VERSION     DASYJA010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602353"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="VGO02_08490"
     CDS             complement(<1..1116)
                     /locus_tag="VGO02_08490"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEV7477757.1"
                     /translation="MDQTLDVKKAIRDLGVAARAASRLLARAGTAAKNQALSAAASAI
                     RSSQKKILEANKLDVAGAKKAGRDAAFIDRLALSAELVEQMAEGVEQVAALADPVGAI
                     SERVTRPTGIEVARMRVPIGVIALIYESRPNVTADAAALCVKSGNACILRGGSESLHS
                     NRVIAAGMRSGLKAAGLPESAVQLVEFSDRAAVGELITLTEYVDILVPRGGKELIARL
                     TRESRIPMIKHLDGVCHVYVDDSADPAMAVRIADNAKTQRYGTCNTMETLLVAEGIAR
                     KVLPELGRIFLSKKVEMHGDDATQKLIPGIKKATDSDYYTEWLAPVVSIRVVKGLDEA
                     IEHIAKYGSQHTDAIVAGDRARAERFLREVDSSSVMWN"
     gene            complement(1100..2068)
                     /gene="holA"
                     /locus_tag="VGO02_08495"
     CDS             complement(1100..2068)
                     /gene="holA"
                     /locus_tag="VGO02_08495"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV7477758.1"
                     /translation="MQLRAEQLEGHLAKALAPAYAIHGDEPLLALEAVDALRAAARSR
                     GFTERQVFEPGRGFDWSEFAHATQSLSLFAEKKVLELRLPSGKPGTQGGEAIATYCAQ
                     PAADLLLLVSVPRLDRATQNSAWFGALGRAGVVLDIWPVERARLPAWIAARLARQRQS
                     ASPEVLEFLADRVEGNLLAAHQEVRKLSLVAGEGALTLEAVQEAVSNVARYDPNDAAE
                     ALLAGDLARYARVIEGLRGEGEAPPYLLFAVSSALFALQRGERVFNRRVQAAVEAAAR
                     RYTPARIEQALAHTAAIDRAIKGVGAGEPWQEFLALGLKLTHGSNA"
     gene            complement(2068..2556)
                     /gene="lptE"
                     /locus_tag="VGO02_08500"
     CDS             complement(2068..2556)
                     /gene="lptE"
                     /locus_tag="VGO02_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEV7477759.1"
                     /translation="MRILSALLCLLLAACGFQLRGTADVPFQTIYVPSSGGGIGLELR
                     RNIQAGTKAKVVDDAKQAEAVLEFTQEAREKEILSLTGTGRVREFQLRYRVGFRVHDG
                     KGREFLPVNTVVLVRDISFNDTDVLAKEAEEQLLYRDMQSDMVQQILRRLAAAKPAAQ
                     AQ"
     gene            complement(2556..>2758)
                     /locus_tag="VGO02_08505"
     CDS             complement(2556..>2758)
                     /locus_tag="VGO02_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477760.1"
                     /translation="ALEQDEIEYVVQINGVKRGSVRAPKAADKTALEVLVTSLDFVKK
                     YLGEKIIKRIVIVPGRLINIVV"
BASE COUNT          386 a          979 c          933 g          460 t
ORIGIN      
        1 gttccacatc accgagctcg agtcgacctc gcgcaggaag cgctcggcgc gcgcgcggtc
       61 gccggcgacg atggcatcgg tgtgctgcga gccgtacttg gcgatgtgct cgatcgcctc
      121 gtcgaggccc ttgacgacgc gtatcgacac caccggcgcg agccattccg tgtagtagtc
      181 actgtcggtg gccttcttga tcccggggat cagcttctgc gtcgcgtcgt cgccgtgcat
      241 ctccaccttc ttcgacagga agatcctgcc gagctcgggc aggaccttcc tggcgatgcc
      301 ctcggccacc agcagcgtct ccatcgtgtt gcaggtgccg tagcgctgcg tcttcgcgtt
      361 gtcggcgatg cgcaccgcca tcgccggatc ggcgctgtcg tcgacgtaga cgtggcagac
      421 gccgtcgagg tgcttgatca tcgggatgcg cgactcgcgc gtcagccgcg cgatcagctc
      481 tttcccgcct cgcggcacta gtatgtccac gtattcggtc agcgtgatca gctcgcccac
      541 cgcggcgcgg tcggagaact cgaccagctg caccgcgctc tccggcaacc cggcggcttt
      601 caggccgctg cgcatgccgg cggcgatgac gcggttcgaa tgcagcgact cggagccgcc
      661 gcgtaggatg caggcgttgc ccgacttgac gcacagcgcg gcggcatccg ccgtcacgtt
      721 cggccgcgac tcgtagatga gggcgatgac gccgatcggc acgcgcatgc gcgccacttc
      781 gatgccggtt ggccgcgtca cccgctcgga gatcgcgccg accgggtcgg cgagcgccgc
      841 cacctgctcg acgccttcgg ccatctgctc caccagctcg gcgctcagcg ccaggcggtc
      901 gatgaaggcg gcgtcgcggc cggccttctt cgcgccggcg acgtcgagct tgttcgcctc
      961 gaggatcttt ttctggctcg agcggatggc cgacgcggct gcgctcagcg cctggttctt
     1021 cgcggcggtc ccggctcgcg ccagcaggcg cgacgcggcg cgggccgcca cgcccaggtc
     1081 gcgaatcgcc ttcttgacgt caagcgtttg atccatgagt caattttaga ccaagcgcga
     1141 ggaactcctg ccacggctcg ccggcgccca cgcccttgat cgcgcggtcg attgcagcgg
     1201 tgtgcgcgag cgcctgctcg atgcgcgcgg gggtgtagcg ccgcgcggcc gcctccaccg
     1261 cggcctgcac ccgccggttg aacacgcgct cgccgcgctg cagcgcgaac agcgccgagg
     1321 acaccgcgaa cagcaggtag ggcggcgcct cgccctcgcc gcgcaggccc tcgatcacgc
     1381 gtgcatagcg ggcaaggtcg ccggcgagca gcgcctcggc cgcgtcgttc gggtcgtagc
     1441 gcgcgacgtt gctcaccgct tcctgcaccg cctcgagcgt gagcgcgcct tcgcccgcga
     1501 cgagggacag cttcctcacc tcctggtgcg cggccagcag gttgccctcg acgcggtcgg
     1561 cgaggaactc gagcacctcg ggcgacgcgc tttggcgctg gcgggcgagg cgcgcggcga
     1621 tccacgccgg cagccgggcg cgctcgaccg gccagatgtc gagcaccacg ccggcgcggc
     1681 cgagcgcgcc gaaccaggcc gagttctgcg tcgcgcggtc caggcgcggc acggatacga
     1741 gcagcagcag gtctgcggcc ggctgcgcgc agtaggttgc gatcgcctcg ccgccctgcg
     1801 tgcccggctt gcccgagggc aggcgcagct cgaggacctt cttctcggcg aacagcgaca
     1861 ggctctgcgt ggcgtgcgcg aactcgctcc agtcgaagcc gcgccccggc tcgaacacct
     1921 ggcgctcggt aaaaccgcgg ctgcgcgccg ccgcgcgcag cgcgtcgacg gcctcgagcg
     1981 cgagcagcgg ctcgtcgccg tggatcgcgt aggccggcgc gagagccttg gcgaggtggc
     2041 cttcgagctg ctcggcgcgc agctgcacta ttgggcctgc gccgccggct tggcggcggc
     2101 gaggcgccgc aggatctgct gcaccatatc gctttgcatg tcgcgataca gcagctgctc
     2161 ctccgcctcc ttcgccagca cgtcggtgtc gttgaagctg atgtcgcgca ccagcaccac
     2221 cgtgttaacc ggcaggaatt cgcggccctt gccgtcgtgc acgcggaagc cgacgcggta
     2281 gcgcagctgg aattcgcgca cgcggccggt gccggtgagc gagagaatct ccttctcgcg
     2341 cgcctcctgc gtgaactcga gcaccgcctc ggcctgcttg gcgtcgtcga ccaccttggc
     2401 cttggtgccc gcctggatgt tgcgcctcag ctcgaggccg atgccgccgc cagaagacgg
     2461 cacgtagatg gtctggaacg gcacgtccgc cgtgccgcgc agctggaagc cgcaggcggc
     2521 gagcagcaag cagagcaggg cggagaggat tctcatcaca ccacgatatt aatcagtcgc
     2581 ccggggacga tgacgatgcg cttgatgatc ttttctccga ggtatttttt tacgaaatcg
     2641 agcgacgtca ccagaacttc caaagctgtc ttgtcggcgg ccttaggcgc tcgcacgctg
     2701 ccgcgcttca caccgttgat ctgcacgaca tactcgatct cgtcctgctc gagggcct
//
LOCUS       DASYJA010000386         4510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_512946, whole genome shotgun sequence.
ACCESSION   DASYJA010000386 DASYJA010000000
VERSION     DASYJA010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512946"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1651
                     /gene="hscA"
                     /locus_tag="VGO02_08510"
     CDS             <1..1651
                     /gene="hscA"
                     /locus_tag="VGO02_08510"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEV7477761.1"
                     /translation="QARQADDPKNTVVSVKRFMGRGVKDVDHLQNAPYDFVDAPGMLQ
                     IRTAAGVKSPVEVSAEILKVLRERAELSLQGNLSGAVITVPAYFDEAQRQATKDAGRL
                     AGLNVLRLLNEPTAAAIAYGLENASEGIYAVYDLGGGTFDLSILKLSRGVFEVVATSG
                     DSMLGGDDFDQRVFCWAVEQAKLPPLGARDMRLLQMKAREAKEYLTFHGEAPLTAKLS
                     SGEVVDLSLDTETFCSITQTLVNKTLGPVRKALRDAGLTVEEIKGVVMVGGATRMPQV
                     QRAVAEFFRRDPLNNLDPDKVVALGAAMQANVLAGNQPTGQDWLLLDVIPLSLGVETM
                     GGLAEKVIARNTTIPAAKAQEFTTFKDGQTGMSFHVVQGERELVADCRSLARFELKGI
                     PPMAAGAARVRVTYQVDADGLLQVSARENSSGVEASVTVKPSYGLVDADIERMLRDSF
                     EHAKEDVHARALAEYRVDGRRLLEATRSALLQDEKLLSAEELESIRKAVLSLEGLLAG
                     TDHRAIKQAVDALNRATEEFAQRRMDSGIRRALSGKKIGSL"
     gene            1651..1998
                     /gene="fdx"
                     /locus_tag="VGO02_08515"
     CDS             1651..1998
                     /gene="fdx"
                     /locus_tag="VGO02_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEV7477762.1"
                     /translation="MPKLKVLPHAALCPNGADLSVQTGKSLCDALLDNHIEIEHACEK
                     SCACTTCHVVVRQGFDSLDEAEEKEEDMLDKAWGLEATSRLSCQAKMGDADLIIEIPK
                     YTINYEREGGAKK"
     gene            1995..2189
                     /gene="iscX"
                     /locus_tag="VGO02_08520"
     CDS             1995..2189
                     /gene="iscX"
                     /locus_tag="VGO02_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEV7477763.1"
                     /translation="MKWTDTREIAIALCEKFPDVDPQTVRFTDLHKWVMALPGFDDQP
                     NRSGEKILEAIQMTWIDEAE"
     gene            2245..2820
                     /locus_tag="VGO02_08525"
     CDS             2245..2820
                     /locus_tag="VGO02_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEV7477764.1"
                     /translation="MRLLFCLLLLAASGSSMAQVYRWVDAKGTVHYSNEKPAADVKAT
                     VVNIDAKTGPASPDTLDCYTVRCQGERLEQRLARREELEARDAEQRTAAAPKPARGLE
                     FRKYISIQRGMSEGELLGIAGAPDLLVDNGGAWFPAPAAVQSGRNVRSGAIVGGAMRT
                     YTYMPTSADPFTTTITLIGGRVSEMERIRKF"
     gene            2868..3059
                     /locus_tag="VGO02_08530"
     CDS             2868..3059
                     /locus_tag="VGO02_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477765.1"
                     /translation="MASKDKVEGEGSYSGTKDYNQRTKKFIESGKVEQAAEDAKPESE
                     QQAHEMHKAERIGKQKAKK"
     gene            complement(3075..3668)
                     /gene="msrA"
                     /locus_tag="VGO02_08535"
     CDS             complement(3075..3668)
                     /gene="msrA"
                     /locus_tag="VGO02_08535"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEV7477766.1"
                     /translation="MNRWILLLAFLLPLAAQAQKTEKAAFAAGCFWCTEEAFEKVPGV
                     ISAVSGYMGGKTQHPTYEQVSRGITGHTEMVEVTFDPAKVSYEKLLDVFWVNHDPTVL
                     DRQFCDGGSQYRPEIFYYSDEQKRLADASKAKWEKAKPFRQPILTPITKAGPFWPAED
                     YHQDYYKKNALQYKFYVTGCGRYARLDSLWGSLRNSK"
     gene            complement(3665..4147)
                     /locus_tag="VGO02_08540"
     CDS             complement(3665..4147)
                     /locus_tag="VGO02_08540"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEV7477767.1"
                     /translation="MVRGNLLEEPVDAIVNAANGHLAHGGGVAGIIARAAGPALQEES
                     DRLVRTQGPFSTGEAVVTTAGKLPFKGVIHAVGPRFGEGDEEAKLVQALLSCWQCAIE
                     RGWTTVSFPAVSSGIFAVPLDICARAYLAAAHRARLSNVRLCLRDQPVIDAVLKELGT
                     "
     gene            complement(4204..>4510)
                     /locus_tag="VGO02_08545"
     CDS             complement(4204..>4510)
                     /locus_tag="VGO02_08545"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV7477768.1"
                     /translation="DELATKNSKDAGTAQRGGDLDWNVPGAYDRVFSEAMVKLEKGKY
                     TEVPVQTRFGYHVILLDDVRPVKFPTLAEVKPRLQQQLVQGKIEELVKGLRAKAKIE"
BASE COUNT          799 a         1486 c         1520 g          705 t
ORIGIN      
        1 gcaggcgcgc caggccgacg atccgaagaa caccgtcgtc tcggtgaagc gcttcatggg
       61 acgcggcgtg aaggacgtcg accacctgca gaacgcgccc tacgacttcg tcgacgcgcc
      121 cggcatgctg cagatccgca ccgccgccgg cgtcaagagc ccggtcgagg tgtccgccga
      181 aatcctcaag gtcctgcgcg agcgcgcgga gctgtccctg cagggaaatc tctcgggagc
      241 cgtcatcacg gttcccgcgt acttcgacga ggcgcagcgc caggcgacga aggacgcggg
      301 gcgcctcgcc ggcctgaacg tccttcggtt gctgaatgag cccacggcgg cggcgatcgc
      361 gtacgggctg gagaatgcgt ccgaaggcat ctacgccgtc tacgacctcg gcggcggcac
      421 gttcgacctg tcgatcctca agctctcgcg cggcgtgttc gaggtggtgg cgaccagcgg
      481 cgactcgatg ctcggcggcg acgatttcga ccagcgcgtg ttctgctggg ccgtcgagca
      541 ggcgaagctg ccgccgctcg gggcgcgcga catgcgcctg ctgcagatga aggcgcgcga
      601 ggccaaggag tacctcacgt tccacggcga ggcgccgctc accgccaagc tctccagcgg
      661 tgaggtggtg gatctgtccc tggacacgga aacgttctgc agcattacgc agactctggt
      721 caacaagacg ctcggcccgg tgcgcaaggc gctgcgcgac gccggcctga cggtggagga
      781 gatcaagggc gtggtgatgg taggtggcgc gacgcgcatg ccgcaggtgc agcgcgcggt
      841 ggccgagttc ttccgccgcg acccgctcaa caacctcgac cccgacaagg tggtggcgct
      901 gggtgccgcg atgcaggcga acgtgctcgc cggcaaccag ccgaccggcc aagactggct
      961 gctgctcgac gtcatcccgc tctcccttgg cgtcgagacc atgggcggcc tggccgagaa
     1021 ggtcatcgcg cgcaacacca ccatcccggc ggcgaaggcg caggagttca ccaccttcaa
     1081 ggacggccag accggcatga gctttcacgt cgtgcagggc gagcgcgagc tggtcgctga
     1141 ctgccgctcg ctcgcgcgct tcgagctgaa gggcatcccg ccgatggccg ccggcgcggc
     1201 gcgcgtgcgc gtcacctatc aggtcgacgc cgacggcctg ctgcaggttt cggcgaggga
     1261 gaattcctcc ggagtggagg cctccgtcac ggtcaagccg tcctacggcc tggtcgacgc
     1321 cgacatcgag cgcatgctgc gcgattcctt cgagcacgcg aaggaggacg tccatgcgcg
     1381 cgcgctcgcc gagtaccgcg tcgacgggcg gcgcctgctg gaggccaccc gttccgcgct
     1441 tttgcaggac gagaaattgc tgagcgccga ggagctcgaa tcgatcagga aagccgtgct
     1501 ttcgctcgag ggattgctgg ccgggaccga ccaccgcgcc atcaagcagg cggtcgacgc
     1561 gctcaaccgc gccacggagg agttcgcgca gcgccgcatg gacagcggca tccgccgcgc
     1621 gctgtcgggc aagaagatcg gcagcctctg atgcccaagc tgaaggtgct gccgcatgca
     1681 gcgctgtgcc cgaacggtgc cgatttgagc gtgcagaccg gaaaatcgct ctgcgacgcg
     1741 ctgctcgaca atcacatcga gatcgagcac gcctgcgaga agtcctgcgc gtgcacgacc
     1801 tgccacgtcg tcgtgcgcca gggcttcgac tcgctcgacg aggccgagga aaaggaggag
     1861 gacatgctcg acaaggcctg gggcctcgag gccacctcgc gcctgtcgtg ccaggcgaaa
     1921 atgggcgacg cggacctgat catcgagatc ccgaagtaca ccatcaacta cgagcgggaa
     1981 ggcggcgcca agaaatgaag tggaccgaca cgcgcgagat cgccatcgcc ctgtgcgaga
     2041 aattccccga cgtggacccg cagacggtgc gcttcaccga cctgcacaag tgggtcatgg
     2101 cgctgcccgg gttcgacgac cagcccaacc gcagcggaga gaaaatcctc gaggcgatcc
     2161 agatgacctg gatcgacgaa gcggagtagc tttcgggtaa caagttggta cagatagcga
     2221 ttccctagcg gtagacttgg cgcaatgcgg cttcttttct gcctgctgct cctggctgcc
     2281 tcgggttcct cgatggcgca ggtctaccgc tgggtggacg ccaagggcac ggtgcactac
     2341 tccaacgaga agccggcggc cgacgtcaag gcgacggtgg tcaacatcga cgccaagacc
     2401 ggcccggcgt cgcccgacac gctcgactgc tacacggtgc gctgccaggg cgagcgcctc
     2461 gagcagcggc tcgcacggcg cgaggagctg gaggcgcgcg atgcggagca gcgcaccgcg
     2521 gccgcgccga agccagcgcg cggcctggag ttccgcaagt acatttccat ccagcgcggc
     2581 atgagcgaag gcgagctgct cggcatcgcc ggcgctcccg acctgctggt cgacaacggt
     2641 ggagcctggt ttccggcccc ggcggcggtg caaagcggcc gcaacgtacg cagcggcgcg
     2701 atcgtcggcg gggcgatgcg cacctacacc tatatgccga cctccgccga tccgttcacc
     2761 acgacgatca cgctgatcgg cggacgcgtc agcgagatgg agcgtatccg caagttctag
     2821 gcacgccgat tgctcgtgga ggaggagttc tctccaggag gcgaatcgtg gcaagcaagg
     2881 acaaggtcga aggcgaaggc agctacagcg gcacgaagga ctacaaccag cggaccaaga
     2941 aattcatcga gtcgggcaag gtcgagcagg cggccgagga cgcaaagccg gaatccgagc
     3001 agcaagcgca cgagatgcac aaggccgagc gcatcggcaa acagaaagcc aaaaagtagc
     3061 gcggggcggt ttccttactt gctgttgcgc aacgatcccc aaagggagtc gaggcgcgcg
     3121 tagcgcccgc agccggtaac gtagaacttg tactgcagcg cgttcttctt gtagtagtcc
     3181 tgatggtagt cctcggccgg ccagaacgga cccgccttgg tgattggcgt gaggatcggc
     3241 tggcggaatg gcttcgcctt ttcccatttc gctttcgagg cgtcggctag gcgcttctgc
     3301 tcatccgagt agtagaagat ctcgggccgg tattgcgagc cgccgtcgca gaactggcgg
     3361 tcgagtaccg tgggatcgtg gttcacccag aaaacgtcga gcagcttctc gtagctcacc
     3421 ttggccggat cgaacgtcac ctccaccatc tcggtatggc cggtgatgcc gcgcgatacc
     3481 tgctcgtagg tgggatgctg cgtcttgccg cccatgtagc cggacaccgc cgagatgacc
     3541 ccgggcacct tctcgaacgc ttcctcggtg caccagaagc agccggcggc gaacgccgcc
     3601 ttctccgtct tttgcgcctg cgccgcgagc ggcagcagga aagccagcag caggatccac
     3661 ctgttcatgt tccaagctcc ttgagtaccg cgtcgatgac cggttggtcg cgcaagcata
     3721 gccggacatt actgagcctg gcgcggtgcg ccgccgccag gtaggcgcgg gcgcagatgt
     3781 cgagcggcac ggcgaagatg ccgctcgaga ccgccggaaa ggacaccgtg gtccagcctc
     3841 gctcgatcgc gcactgccag caggaaagca gggcctgaac cagcttggct tcctcgtcgc
     3901 cctcgccgaa gcggggcccc acggcgtgga tcacgccctt gaacggcagt ttgccggccg
     3961 tggtgacgac tgcctccccg gtgctgaagg gaccctgggt gcgcaccagc cggtcggatt
     4021 cctcctgcag cgccgggccg gctgcgcgcg cgatgatgcc ggctacgccg ccgccgtgcg
     4081 ccaggtggcc gttggccgcg ttgacgatgg cgtccaccgg ctcctcgagc aggttgccgc
     4141 gcaccaccag aaacaaggcg ccggcgccgg tctgctcgcg atgcagctcc tgcgccgtgg
     4201 cggctactcg atcttcgcct tggcgcgcag gcccttcacc agctcctcga tcttcccctg
     4261 caccagctgc tgctgcaggc gcggcttcac ctcggcgagc gtcgggaact tcaccgggcg
     4321 cacgtcgtcg agcaggatca cgtgatagcc gaagcgcgtc tgcaccggca cttcggtgta
     4381 cttgcccttc tcgagcttca ccatggcttc cgaaaacacg cgatcgtagg cgcccggcac
     4441 gttccagtcg aggtcgccgc cgcgctgggc ggtgccggcg tccttcgagt tcttggtggc
     4501 gagctcgtcg
//
LOCUS       DASYJA010000387         3038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_785354, whole genome shotgun sequence.
ACCESSION   DASYJA010000387 DASYJA010000000
VERSION     DASYJA010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785354"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VGO02_08550"
     CDS             <1..290
                     /locus_tag="VGO02_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477769.1"
                     /translation="AGRWMMGGCGLLWDEREARWRRPVPLGVIAGTRPLGLGRAFGRL
                     PGENDGVVCVSETTVDGMTARALVPQGHSMLIVSGQVGRLVERFLSSARFE"
     gene            287..1294
                     /locus_tag="VGO02_08555"
     CDS             287..1294
                     /locus_tag="VGO02_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEV7477770.1"
                     /translation="MKNLLLFVLVLTAGCESLSYYSQAIRGQLGLMGAARPVDAWLAD
                     PATPPELRSRLQVAQRIREFGSQRLALPQNGSYGSYAELDRPYAVWNVYAAGRFSVDP
                     KQECFPFTGCVSYRGFFSEEQARRHAARLREEGYDVYLGGVPAYSTLGWFDDPLLSTF
                     IKYPDVQLARLVFHELAHQRVYLKGDTTFNESFAVTVEEEGVRRWLETAGRGGELAAL
                     RAARLRRQEFAARVSQARERLAQVYQEKIPEEAMLARKREEYDKLRADYPGIVPAEPN
                     NAYLVSIALYTQLVPDFERLLAACGGDLAEFYDRVEALAAASRREARLALTSAGCRRP
                     A"
     gene            complement(1263..1880)
                     /locus_tag="VGO02_08560"
     CDS             complement(1263..1880)
                     /locus_tag="VGO02_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEV7477771.1"
                     /translation="MLKIWGRVNSVNVKKALWAAEELGLKYERVDAGMQFGVTKTPEY
                     LRMNPNSLVPTIEDDGFVLWESGAVVRYLCAKHSMGKLCPSDLRARADAERWMDWAFT
                     FQSAMRNVFWGLIRTPPEKRDHKAIEEGRVQSARLAEVLERNLEKKPYVAGDAFTMGD
                     IPIGCEVQRWMRVPIERPALPRVQAWFDRLRERAPFRQVVDIPLT"
     gene            complement(1880..>3038)
                     /locus_tag="VGO02_08565"
     CDS             complement(1880..>3038)
                     /locus_tag="VGO02_08565"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEV7477772.1"
                     /translation="GRKGFLVSPTIAGQWANQVPELINQPGFAKSFLPDGRPPHPGER
                     FKFPAHARALEEIAASKGKAFYRGKLADKLEAAAKKQGGALRASDMAEHESDWVKPLE
                     MDYRGYTLHEIPPNGQGIVALMALGMLEHFDLRSHPVDGPDSLHLQIEAQKLAFADAR
                     AFVADVDYMKDVTPESLLNKSYLRERAKLIDLKRAQDFGHGTPPRSGTVYLSAADASG
                     MMVSFIQSNYMGFGSGVVVDGISLQNRGGTFVVQPGHPNCVGPGKRPYQTIIPGFVTK
                     DRKPVMSFGVMGGTMQPQGHAQVMVRIADYAQSPQAACDGARFRVVQGLDVSVEDGFP
                     AASLEELKRRGHRIVTVDDYNQFGCAQLIWKLDGGYFAASDPRRDGQAVGF"
BASE COUNT          450 a         1053 c         1001 g          534 t
ORIGIN      
        1 acgccgggcg ctggatgatg ggcggttgcg gcctcttgtg ggacgagcgc gaggcgcgct
       61 ggcggcgtcc ggtgcccctc ggcgtcatcg ccggcacgcg gccgctgggc ctgggccgcg
      121 cctttggccg gctgccgggc gagaacgacg gcgtggtttg cgtgtccgag actactgtcg
      181 acggcatgac tgcccgggcg ctggtgcccc agggccatag catgctgatc gtttccggac
      241 aggtgggcag gctggtggag cggtttctct ccagcgcgcg gttcgaatga aaaatttact
      301 cctcttcgta ctcgtcttga ctgcaggctg cgaatcgctt tcctactatt cgcaggcgat
      361 ccgcggccaa ctcggcctga tgggcgcggc gcgcccggtc gacgcctggc tcgccgaccc
      421 ggccacgccg cccgagctgc gcagccgcct gcaggtggcg cagcgcatcc gggagttcgg
      481 ctcgcagcgc ctggcgctgc cgcagaacgg cagctacggc tcctacgccg agctcgaccg
      541 gccctacgcg gtctggaacg tgtatgccgc ggggcgcttt tccgtcgatc cgaagcagga
      601 gtgctttccg ttcaccggct gcgtcagcta ccgcggcttc ttctccgagg aacaggcgcg
      661 ccgccatgcc gcgcgcctgc gcgaggaggg ctacgacgtc tacctcggcg gcgtgccggc
      721 gtactcgacg ctcggctggt tcgacgaccc gctgctctcc accttcatca agtaccccga
      781 cgtgcagctg gcgcggctcg tgttccacga gctcgcgcac cagcgcgtct acctgaaggg
      841 cgacaccacg ttcaacgaat ccttcgccgt gacggtggag gaggaggggg tgcggcgctg
      901 gctcgagacc gcaggccgcg gcggcgagct cgcggcgctg cgcgccgcgc ggctgcggcg
      961 gcaggagttc gccgcgcggg tgtcccaggc acgcgagcgc ctggcccagg tgtaccagga
     1021 aaagattccg gaagaggcga tgctggcgcg caagcgcgag gaatacgaca agctgcgcgc
     1081 cgactacccg ggaatcgtgc ccgccgagcc gaacaacgcc tacctcgtct cgatcgcact
     1141 ctacactcag ctggtgcccg acttcgagcg gctgctcgcc gcctgcggcg gcgacctggc
     1201 cgagttctac gaccgggtcg aggcgcttgc cgccgccagc cggcgcgagg cgcgcctcgc
     1261 gctcacgtca gcgggatgtc gacgacctgc ctgaacggcg cccgctcgcg cagccggtcg
     1321 aaccacgcct gcacccgcgg caacgccgga cgctcgatcg gcacgcgcat ccagcgctgc
     1381 acctcgcagc cgatcgggat gtcgcccatg gtgaaggcgt cgccggccac gtagggcttt
     1441 ttctccagat tccgctcgag cacctcggcc agccgcgcgc tctgcacccg gccttcctcg
     1501 attgccttgt ggtcgcgctt ctccggtggc gtgcggatca gtccccagaa cacgttgcgc
     1561 atggcgctct ggaaggtgaa ggcccagtcc atccagcgct ccgcgtcggc gcgcgcccgg
     1621 agatccgacg ggcagagctt gcccatgctg tgcttcgcgc acagatagcg caccaccgca
     1681 cccgactccc acagcacgaa gccgtcgtcc tcgatcgtcg gcaccagcga attgggattc
     1741 atccgcaggt actcgggcgt cttggtgacg ccgaactgca tgccggcgtc gacgcgctcg
     1801 tacttcaggc cgagctcctc cgccgcccat agcgcttttt ttacattgac tgaattcaca
     1861 cggccccaga tctttagcat cagaacccca ctgcctgacc atcgcgccgc gggtcgctcg
     1921 cggcgaaata gccgccgtcg agcttccaga tgagctgcgc gcagccgaac tggttgtagt
     1981 cgtcgaccgt cacgatgcga tgcccgcggc gcttgagctc ttcgagcgat gccgccggga
     2041 agccgtcctc gacgctgacg tcgagcccct gcacgacgcg gaaacgcgcg ccgtcgcacg
     2101 ccgcctgcgg gctctgcgcg tagtcggcga tgcgtaccat cacctgcgcg tgcccctgcg
     2161 gctgcatggt gccgcccatg accccgaagc tcatcaccgg cttgcgatcc ttcgtcacga
     2221 agcccgggat gatcgtctgg tacggccgct tccccggtcc gacgcagttc gggtggccgg
     2281 gctgcacgac gaacgtgccg ccgcggttct gcagcgagat gccgtcgacc accacgccgg
     2341 agccgaagcc catgtagttc gactggatga aggaaaccat catgccggag gcgtcggcgg
     2401 cgctgaggta caccgtgccg ctgcgcggcg gggtgccgtg gccgaagtcc tgagcacgtt
     2461 tcaggtcgat cagcttggct ctctctctta aatatgattt attcaagagg ctttctggtg
     2521 ttacatcctt catgtagtcg acatcggcaa cgaaggcgcg cgcgtcggcg aaggcgagct
     2581 tctgcgcctc gatctgcagg tgcaggctgt cgggcccgtc caccgggtgc gagcgcaggt
     2641 cgaaatgctc gagcatgccg agcgccatca gcgcgacgat gccctggccg ttgggcggga
     2701 tctcgtgcag cgtgtagccg cggtagtcca tctcgagcgg cttcacccaa tccgactcgt
     2761 gctctgccat gtcggatgcg cgcagcgcgc cgccctgttt cttcgccgcg gcttccagct
     2821 tgtccgccag ctttccccgg tagaacgctt ttcctttcga cgctgcaatt tcttccagcg
     2881 ccctggcgtg cgccggaaac ttgaatcgct cccctgggtg gggagggcga ccgtccggca
     2941 ggaaagactt ggcgaagcca ggttgattga ttaattcggg gacctggttg gcccattgcc
     3001 ccgcaatggt cggcgaaacg aggaagccct tgcgcccg
//
LOCUS       DASYJA010000388         5484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3236513, whole genome shotgun sequence.
ACCESSION   DASYJA010000388 DASYJA010000000
VERSION     DASYJA010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3236513"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="VGO02_08570"
     CDS             <1..1198
                     /locus_tag="VGO02_08570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEV7477773.1"
                     /translation="RVNPQKARNIEDLRRLARRRLPRIVRDYIEGGAEDHVTLRANRE
                     SLEAIRFAPRALVDVSRRSQKTVLFGREFGAPFGIAPIGAAGLVWHEAEIALARAARA
                     ANLPSVLSTHSFVPLARVARHAGGAPWFQLYLPNNRIDAGNLVALALDAGCEALVLTV
                     DVPVGGNREYNERNGFRMPLRLGPRTLLDGALHPRWLAGVYLRSNWRGKLAEWGTRRD
                     TQTWQDLDWLRRAWPRRLLVKGILTPEDALLALERGADGIFVSNHGGRQLDGAPAAIE
                     ALPPIAAAVGGRMAVLVDGGFRRGADLVKALALGADMAFVGRAALYGAAAGGEAGVRR
                     ALHILKSEVDRVLALLGCASSADLGPRHVRLAGRALFGLDRGRAGDVREQRDFLADQP
                     VELLRR"
     gene            complement(1109..2074)
                     /locus_tag="VGO02_08575"
     CDS             complement(1109..2074)
                     /locus_tag="VGO02_08575"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7477774.1"
                     /translation="MTSQKVPALIALVALLAASPVSSQDFPNRSLRIVVGFTPGGGPD
                     LTARHIAQKLAESLKQQVLVENRPGAGGTLAAAQVARAAPDGYTLLSVSSAHAAAAAI
                     YTKLPYETKELAGVTQTGSSKYVLVVAPSLKMNSLAQLIAEAKASPGRINFSSAGVGS
                     GTHFAGEIFKSMAAIDVVHIPFKGIPEALTEVLTGRVHFFMAPIANSVAQVKDGRLLG
                     LGVSSLARDPLLPEVPTIDEGGVRGYQSELWFGLLAPAATPQPVLARLNEEIARILRD
                     ADIRARWAPIGIEPRPSTPQEFDRLIREEIALFTNIARAASIKAE"
     gene            2046..2195
                     /locus_tag="VGO02_08580"
     CDS             2046..2195
                     /locus_tag="VGO02_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477775.1"
                     /translation="MSAGTFWLVMSLAVAVGLIGALFLSGWRHRKDRMPQVPPLPPEE
                     DDWRK"
     gene            complement(2205..2333)
                     /locus_tag="VGO02_08585"
     CDS             complement(2205..2333)
                     /locus_tag="VGO02_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477776.1"
                     /translation="MDAPKKSKPWAEMSGNQKFAFVCKLVLSIVSFGFIYPNLMND"
     gene            complement(2414..2650)
                     /locus_tag="VGO02_08590"
     CDS             complement(2414..2650)
                     /locus_tag="VGO02_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477777.1"
                     /translation="MGNLHPAIEGAIVGVVIAIVLYGLEYYLLKKQAKERAIRQHKPN
                     VVEFDSTEKARLNSVRNMCAFVPVFFAAMYWMIS"
     gene            complement(2660..3313)
                     /locus_tag="VGO02_08595"
     CDS             complement(2660..3313)
                     /locus_tag="VGO02_08595"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEV7477778.1"
                     /translation="MNWLYQVARKPAELFFPVSGVLFKTPEQTWSQYAPIFRRHATTT
                     VTPELLAALAQVEGAGNPVARTYWRWRLTHEPFEIYRPASSAVGMYQITDGTFREARR
                     YCVRDHQVIEDGPWNDWKSCWFNSLYLRVVPSHAVEMTAALLDRQVASIAANAAAAQK
                     QDLAAVIHLCGAAGGAAYAARGFRAAPGQRCGDHDLRAYLARVNAMKRMFARFAALS"
     gene            3459..3695
                     /locus_tag="VGO02_08600"
     CDS             3459..3695
                     /locus_tag="VGO02_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477779.1"
                     /translation="MGDESLRRVSELVFVHGEAKAILEWIDSAGLRTPIYIDMDRSKL
                     RMTRGIRTLYYYDGTTVDPRGMDLKEPPEQYKPH"
     gene            3789..4874
                     /locus_tag="VGO02_08605"
     CDS             3789..4874
                     /locus_tag="VGO02_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477780.1"
                     /translation="MPYKILIFGASYGSLLATKMALGGHSAKMICLPNEAELFNKEGA
                     IVRMPVKGREGLVQVESRKLPGKITAGGPGGVNPAEYDLVVLGMQEPQYRSAGVRELL
                     DAVAKSKVPTMSIMNMPPLPYLKRIRGIDADAIRPCYTDATVWDSFDPKFMTLCSPDP
                     QAFRPPEEKVNVLQVRLPTNFKSAAFESPAHTAILRKLEADIEAARFEGMELPVKLKV
                     HDSVFVPLAKWAMLLAGNYRCVTKQGIRSIKDAVHSDLAASRAVYEWVVKLCVSLGAE
                     PKDLVPFDKYAAAAQGLASPSSAARALYAGAPNIERVDRLVQTIAATRGMRLAAVDET
                     VALVDAKLEENRRNAGAAAKTKPAQAA"
     gene            5068..5259
                     /locus_tag="VGO02_08610"
     CDS             5068..5259
                     /locus_tag="VGO02_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477781.1"
                     /translation="MNKLLIAAASLMIATSVQANQPEFTLVEVEMTPIAEVQKPIVVA
                     KWDSRFGAYALENIAAGGQ"
BASE COUNT          934 a         1878 c         1821 g          851 t
ORIGIN      
        1 acgtgtgaac ccgcagaagg cgcgcaacat cgaggacctg cgccgcctcg cgcgccgccg
       61 cctgccgcgc atcgtgcgcg actacatcga gggcggcgcc gaggaccacg tgacgctgcg
      121 cgccaaccgc gagtcgctcg aggccatccg cttcgcgccg cgcgccctgg tcgacgtctc
      181 gcgccgctcg cagaaaaccg tgctcttcgg ccgcgagttc ggcgcgccct tcggcatcgc
      241 gccgatcggc gcggccggcc tggtctggca cgaggccgag atcgcgctgg cgcgcgcagc
      301 gcgcgccgcg aacctgccgt cggtgctctc gacgcacagt ttcgtgccgc tcgcccgcgt
      361 ggcgcgccat gccggcggcg cgccgtggtt ccagctgtac ctgcccaaca accgcatcga
      421 cgcgggcaac ctggtcgcgc tggcgctcga tgccggctgc gaagcgctgg tgctgaccgt
      481 cgacgtgccg gtaggaggca accgcgagta caacgagcgc aacggctttc gcatgccgct
      541 gcgcctcggg ccgcgcacgc tgctcgacgg cgcgctgcat ccgcgctggc tcgccggcgt
      601 ctacctgcga agcaactggc gcggcaagct ggcggaatgg ggcacgcggc gcgacacgca
      661 gacctggcag gacctcgact ggctgcggcg cgcctggccg cgcaggctcc tcgtcaaagg
      721 catcctcacc cccgaagacg ccctgctcgc gctcgagcgc ggcgccgacg gcatcttcgt
      781 ctccaaccac ggcggccggc agctcgacgg cgcgcccgcg gcgatcgagg ccctgccgcc
      841 gatcgcggcc gcggtcggcg gccggatggc ggtgctggtg gacggcggct ttcgccgcgg
      901 cgccgacctc gtcaaggcgc tcgcgctcgg cgccgacatg gccttcgtcg gccgtgccgc
      961 gctctacggc gcggcagccg gcggcgaagc cggcgtgcgg cgcgccctgc acatactgaa
     1021 aagcgaagtc gatcgggtgc tggcgctgct cggctgcgcg tcgagcgccg acctcggccc
     1081 gcgccacgtg cgcctggctg gccgcgcgct attcggcctt gatcgaggcc gcgcgggcga
     1141 tgttcgtgaa caacgcgatt tcctcgcgga tcagccggtc gaactcctgc ggcgttgagg
     1201 gccgcggctc gatgccgatc ggcgcccagc gcgcgcggat atcggcgtcg cgcaggatgc
     1261 gcgcgatctc ttcgttcagc cgcgcgagca ccggctgcgg cgtggcggcg ggcgcgagca
     1321 ggccgaacca gagctccgat tgatagccgc gcacgccgcc ctcgtcgatc gtcggcactt
     1381 cgggcagcag cggatcgcgc gcgagcgacg agacgcccag gccgagcagc cgcccgtcct
     1441 tgacctgcgc caccgagttc gcgatcggcg ccatgaagaa atgcacccgg ccggtcagca
     1501 cttcggtgag cgcctccggg atgcccttga acggaatgtg cactacgtcg atcgccgcca
     1561 tcgacttgaa gatctcgccg gcgaagtgcg tgccgctgcc cacccccgcc gaggaaaaat
     1621 tgatgcgtcc cgggctggcc ttcgcctcgg cgatcagctg cgcgagcgag ttcatcttga
     1681 gcgaaggcgc gaccaccagc acgtacttgg agctgccggt ctgggtgacg ccggcgagct
     1741 ccttcgtctc gtacggcagc ttggtataga tcgccgccgc cgcggcatgc gccgaggaca
     1801 ccgacagcag cgtgtagccg tcgggcgccg cgcgcgccac ctgcgccgcg gcgagcgtgc
     1861 cgccggcgcc gggacggttt tccaccaata cctgctgttt caggctttcc gcaagcttct
     1921 gcgcgatgtg acgcgccgtg aggtcgggtc cgccgcccgg cgtgaaaccg actacgatgc
     1981 gcagcgatct attggggaag tcctgcgatg aaaccggcga cgctgcaagc agcgctacga
     2041 gcgcgatgag cgccggaact ttctggctgg tcatgtcgct cgcggtcgcc gtcggcctga
     2101 tcggcgcgct gtttctctct ggctggcggc accgcaagga ccggatgccg caggtgccgc
     2161 cgcttccccc ggaagaggac gactggagga agtgaggagt acggctagtc gttcatcagg
     2221 ttcgggtaga tgaacccgaa cgagacgatc gagagcacca gcttgcagac gaacgcgaac
     2281 ttctgattgc cgctcatctc ggcccagggc ttggacttct tcggtgcatc catcgagcag
     2341 actccctttg gagggtgccg gcgagtctac aggggggtgc agtgcgctgc ctgacggcag
     2401 actgacagcc aagctaggaa atcatccagt acatcgccgc gaagaacacc ggcacgaagg
     2461 cgcacatgtt cctgacggag ttcagccgcg ccttctcggt cgagtcgaat tcgaccacgt
     2521 tcggcttgtg ctggcggatc gctcgctcct tggcctgctt cttcagcagg tagtactcga
     2581 gcccatacag cacgatagcg atgacgacgc cgacgatggc gccttcgatc gcgggatgca
     2641 gattacccat cgacccactc tagcttaagg cggcaaaccg ggcaaacatg cgcttcatcg
     2701 cgtttacccg ggccagataa gcgcgcaggt cgtggtcgcc gcagcgctgg ccgggcgcgg
     2761 cgcgaaaccc gcgcgcggca tacgctgcgc cgccggcggc tccgcacaga tggatcaccg
     2821 ccgccagatc ctgtttctgc gccgccgcgg cgttcgcggc aatcgacgcc acctggcggt
     2881 cgagcagcgc cgcggtcatc tccacagcat ggctgggcac tacgcggaga tagagcgagt
     2941 tgaaccagca ggacttccag tcgttccacg gcccgtcctc gatcacctgg tggtcgcgga
     3001 cgcagtagcg cctggcttcg cggaaggtgc cgtcggtgat ctggtacatg ccgaccgcgc
     3061 tggaagctgg ccggtagatc tcgaacggct cgtgggtcaa ccgccagcgc cagtaggtgc
     3121 gcgcaacggg gttgccggcg ccctcgacct gagccagcgc ggcgagcagc tcaggcgtca
     3181 ccgttgtcgt cgcatggcgg cgaaagatgg gggcgtactg gctccaggtc tgctccggcg
     3241 tcttgaaaag aacgccgctc accggaaaga acagctccgc gggcttgcgg gccacctggt
     3301 agagccaatt cacggcaagg gccgccgcca gcacggcaca ggccgccagg gaatacctcg
     3361 cccagcgcgg tgcggcacgc cacctgctgg tccagcgtat ggagaagaat cgtcgtctca
     3421 tcaggcgcgt agggctacgg gtgatccacc gtcacagcat gggtgacgag tccctgcggc
     3481 gagtttcgga gctggttttc gtgcacggcg aggcaaaagc catcctggag tggatcgaca
     3541 gcgccggatt gcgcacccca atctatatcg atatggaccg gtcgaagctg cggatgaccc
     3601 ggggcatccg taccctctac tactatgacg gcacgacggt cgacccgcgc gggatggact
     3661 tgaaagagcc gccggagcaa tacaagccgc actagcggtg gtttgctgca ccgcacccca
     3721 aagtcctttg gggtcatggt gttagactcc ggttccctgt tctatcccgc attgcgagga
     3781 gcgttttcat gccctacaag atcctcatct tcggcgcctc ctacggctcc ctgctcgcca
     3841 ccaagatggc cctcggcggc cactcggcca agatgatctg cctgccaaac gaggccgagc
     3901 tcttcaacaa ggaaggcgcc atcgtgcgca tgccggtgaa gggccgcgag ggcctggtcc
     3961 aggtcgagtc gaggaagctc cccggaaaga tcaccgccgg cggcccgggc ggcgtcaacc
     4021 cggccgagta tgacctcgtg gtgctcggca tgcaggagcc gcaataccgc tctgccggcg
     4081 tgcgcgagct gctcgatgcc gtcgcgaagt cgaaggtacc gaccatgtcg atcatgaaca
     4141 tgccgccgct gccgtacctc aagcggatcc gcggcattga cgcggacgcg atccgcccct
     4201 gctataccga cgccaccgta tgggacagct tcgatccgaa gttcatgacg ctgtgcagcc
     4261 ccgacccgca ggccttccgc ccccccgagg agaaagtgaa cgtgctgcag gtgcgcctgc
     4321 cgaccaattt caagtcggcg gccttcgagt cgccggcgca caccgcgatc ctgcgcaagc
     4381 tcgaggcgga catcgaggcg gcgcgcttcg aaggcatgga actgccggtg aagctcaagg
     4441 tgcacgactc ggtgttcgtg ccgctcgcca aatgggcgat gctgctcgcc ggaaattatc
     4501 gctgcgtgac caagcagggc atccgctcca tcaaggacgc agtgcacagc gacctcgctg
     4561 cctcgcgcgc ggtttacgag tgggtggtga agctgtgcgt ttcgctcggc gccgagccga
     4621 aggacctggt gcccttcgac aagtatgccg ccgcggcgca gggactcgcc agcccgtcct
     4681 cggccgcgcg cgctctctac gccggcgcgc cgaacatcga gcgcgtcgac cgcctggtgc
     4741 agaccatcgc cgcgaccagg ggcatgcgcc tggcggccgt cgacgagacg gtggcgctgg
     4801 tggacgccaa gctcgaggag aaccgccgca acgcgggcgc cgccgcgaag accaagcccg
     4861 cgcaggccgc ctgacgggcg gcttgcccgg ctaggacagc gcctgacaat gtcgacggcc
     4921 gcccaacaag gccgttgccg ggccgacagg caggccccga aaagcgtgat tttccgcacg
     4981 tttcaggccg tttctggctg gcacaccccg tgcaataggt aaggcaaccc ggctcaccga
     5041 gcctggaaag acaaacggga gatgaaaatg aacaagcttc taatcgccgc cgccagcctg
     5101 atgattgcga cctccgtcca agccaaccag ccggagttca cgctggtcga agtggagatg
     5161 accccgatcg cggaagtgca gaagccgatc gtggtcgcca agtgggactc gcgcttcggt
     5221 gcctacgccc tcgagaacat cgccgccggc ggccagtaac tgaaaacgca gcagacgtag
     5281 aagcagaagc agcagcaagt acctccccgc ctctccaggc gcaacggggc ccggtttcag
     5341 gaaccgggcc ccgttgtctt tacaggtcct gacgaagtcg acgcactgca gacagcgctg
     5401 tcgtgaaccc gacgtcgtac cccctgaatc tgcccaaaaa gtcacgcaaa acaggtggtc
     5461 tggcctggca cacgccgtgc atat
//
LOCUS       DASYJA010000389         1669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1782331, whole genome shotgun sequence.
ACCESSION   DASYJA010000389 DASYJA010000000
VERSION     DASYJA010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1782331"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="VGO02_08615"
     CDS             <1..1026
                     /locus_tag="VGO02_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007733779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEV7477782.1"
                     /translation="CGVRSGVTTLVDQGGPSCMTLPGFREFVVKPKLSRVVTFLSSYL
                     VGGMEGHYYPSLYKPDCVDIDATVKSAEANRDIVKGFKAHAELGGFARWGIEVIKMSA
                     EIARRANLPVYIHFGQLWGLPESGTNGVDPDTILDQVVPLLKPGDILAHPFTRHPGGF
                     VDADGKVHPIVKEALAMGLKTDVGHGSHFSYRMARIALDAGIVPDTLGADMHGYNTRV
                     PRPAGTPEEHSDKDHMFFGSTRFSLVSAMSSMLALGLKLEQVVPMVTSNPAKMLGLER
                     EIGLLKPGVEADVSVLHDERGRWTLSDNEGNQVRAERMLRPAFCLRAGRRHAADASIL
                     PPLAAAA"
     gene            1023..>1669
                     /locus_tag="VGO02_08620"
     CDS             1023..>1669
                     /locus_tag="VGO02_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV7477783.1"
                     /translation="MTAKGVGARLPRKEDRRFLRGEGEYVGNIRLPGMLEVAFVRSPM
                     AHGRIKSIQKPAGVFTADDLKDVKPIRAVSSLKGFKVSEQPILAKDKVRQVGELVAMC
                     VAATRAEAEDLADATELVLEELPAVVDMIEARTKPPALVHEHWGDNVFLESLVDDDIA
                     SVEKKAHIKIKRSIRTARQHMSPMEGRGVVCEWNRRLGQLVMHSAAQMPHINRAGL"
BASE COUNT          293 a          578 c          549 g          249 t
ORIGIN      
        1 tgcggcgtgc gctcgggcgt caccacgctc gtcgaccagg gcggcccctc gtgcatgacg
       61 ctgcccggct tccgcgagtt cgtcgtcaag cccaagctga gccgcgtggt caccttcctt
      121 tcctcctacc tcgtcggcgg gatggaaggg cactactacc cctcgctcta caagccggac
      181 tgcgtcgaca tcgacgcgac ggtgaagagc gccgaagcga accgcgacat cgtgaagggc
      241 ttcaaggcgc acgccgagct cggcggcttc gcgcgctggg gcatcgaggt gatcaagatg
      301 tcggccgaga tcgccaggcg cgcgaatctg ccggtctaca tccacttcgg ccagctctgg
      361 ggtctgcccg agtcgggcac caacggcgtc gatcccgata ccatcctcga ccaggtggtg
      421 ccgctgctca agcccggcga catcctcgcg cacccgttca cgcgccaccc cggcggcttc
      481 gtcgacgccg acggcaaggt gcacccgatc gtgaaggaag cgctggcgat gggcctgaag
      541 accgacgtcg gccacggctc gcacttcagc taccgcatgg cgcgcatcgc gctcgacgcc
      601 ggcatcgtgc ccgacacgct cggcgccgac atgcacggct acaacacgcg cgtgccgcgc
      661 cccgccggca cgcccgagga gcactccgac aaggaccaca tgttcttcgg cagcacgcgc
      721 ttctcgctgg tgtcggcgat gagctcgatg ctcgcgctcg gcctgaagct cgagcaggtg
      781 gtgccgatgg tgaccagcaa cccggcgaag atgctgggcc tggagcgcga gatcggcctg
      841 ctcaagcccg gcgtcgaggc cgacgtcagc gtgctgcacg acgagcgcgg ccgctggacg
      901 ctctccgaca acgagggcaa ccaggtgcgc gccgagcgca tgctgcgccc cgctttctgc
      961 ctgcgcgccg gccggcgcca cgcagccgac gcctcgatcc tgccgccgct cgcggcggcg
     1021 gcgtgaccgc caagggcgtc ggggcgcgtc ttccgcgcaa ggaagaccgc cgcttcctgc
     1081 gcggcgaagg cgagtacgtc ggcaacatcc gcctgccggg catgctggaa gtggcttttg
     1141 tgcgctctcc catggcgcac gggcgaatca agagcattca gaaacctgcc ggggttttta
     1201 ctgctgatga tctgaaggac gtcaagccga tccgggctgt ctccagcctg aaaggcttca
     1261 aggtctccga gcagccgatc ctcgccaaag acaaggtgcg ccaggtcggc gagctggtgg
     1321 caatgtgcgt cgcggcgacg cgcgccgagg ccgaggacct cgccgacgca accgaactgg
     1381 tgctcgaaga acttcccgcg gtcgtcgaca tgatcgaggc gcgcaccaaa ccacctgcgc
     1441 tggtgcacga gcactggggc gacaacgtgt tcctcgaatc attggtcgac gacgatatcg
     1501 cttctgtgga aaaaaaagcc cacataaaaa tcaagagaag catccgcacc gcgcggcagc
     1561 acatgtcgcc gatggagggc cgcggcgtgg tgtgcgagtg gaaccggcgc ctcggccagc
     1621 tggtcatgca cagcgcggcg cagatgccgc acatcaaccg cgcgggact
//
LOCUS       DASYJA010000390         2099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2327962, whole genome shotgun sequence.
ACCESSION   DASYJA010000390 DASYJA010000000
VERSION     DASYJA010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2327962"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..946
                     /locus_tag="VGO02_08625"
     CDS             275..946
                     /locus_tag="VGO02_08625"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEV7477784.1"
                     /translation="MNPVPALAVRAFAATRAMLALIGFAAVAGYLLMPQSARLQSWVA
                     ENAAAESAAGGASTPLQAAAESPLEQEQRAVTEYIAKRYRVSDHAVAGYVSLAYHAGA
                     QQSVDPLLILAVMAIESRYNPVAESSMGARGLMQVIPKYHLEKLLDHGGEPALLEPAV
                     NIAVGAQILREYQRRFRDTETALQMYGGALDDAATQYASKVLAEKARLDALRAKARKQ
                     TAQSV"
     gene            complement(906..2069)
                     /locus_tag="VGO02_08630"
     CDS             complement(906..2069)
                     /locus_tag="VGO02_08630"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV7477785.1"
                     /translation="MPTPTARRADEIEPFEVMDVLSRAHALEAAGRHVVHMEIGEPDF
                     TAPAPVVEAGIRAIRDGLTAYTATLGLPALREAIAAHYQTRFSKKIPPERIAITSGAS
                     GGLLTAAALYVDAGDEILVPDPGYPGYRHFVRAFEGVAKTLPVSAQNNFQPTLEMVRA
                     AWGPKTKGLLLGSPSNPTGTLIDENQLKGIADFIAGRGGVLLVDEIYQGLVYGSEPRS
                     AAGLPGEVVLINSFSKYFCMTGWRLGWVVLPETAVRGFEKLAQHLFISAPSAAQHAAL
                     AAFLPESISILEARRGEFARRRDFLVPALKRSGLSIPAEPHGAFYIYADCGGDARAFA
                     RQLLEKEAVAATPGVDFGANGTARCVRFAYTRSMDDLEEAVRRIARFVCAPSL"
BASE COUNT          345 a          722 c          709 g          323 t
ORIGIN      
        1 cgcgtggccg ttccccaccg gttcccggcc gtaaccggga accaccgggg gacgcaggcg
       61 ggagtgaaga ctcccgccgg ccctcgaagc tcagcggacc ggcttggtca ttcgacccaa
      121 gaacggagac ttaggccggt ccgcgaggct tcccaacgag aatcccattt tacccggggg
      181 ctcctgcgca agcggaactg attgacaaca aaagggaatc caacctatga ttccgtccgc
      241 tgcaccgcac catccagatt cgaaggccct ccccatgaac cccgtacccg cccttgccgt
      301 ccgcgcgttc gccgccaccc gggcgatgct tgccctgatc ggctttgccg ccgtggccgg
      361 gtatctgttg atgccgcaat cggcacggct gcaatcctgg gtcgcggaaa atgcagcggc
      421 cgagagcgcg gcgggcgggg cgagcactcc gctgcaggcc gcggcggaat cgcccctcga
      481 gcaggagcag cgcgcggtca ccgaatacat cgccaagcgc taccgcgtat ccgaccacgc
      541 ggtggccggt tacgtttcgc tcgcctacca tgcaggcgcc cagcaatcgg tcgacccgct
      601 gctgatcctg gcggtgatgg cgatcgagtc gcgctacaac ccggtggccg agagctcgat
      661 gggcgcgcgc ggcctgatgc aggtgatccc gaagtaccat ctcgagaagc tgctcgacca
      721 cggcggcgag ccggcgctgc tcgagccggc ggtcaacatc gcggtgggcg cgcagatcct
      781 gcgcgagtac cagcgccgct tccgcgacac cgagaccgcg ctgcagatgt acggcggcgc
      841 gctcgacgac gcggccacgc agtacgcctc gaaggtgctt gccgagaaag cgcgcctgga
      901 cgcgctcaga gcgaaggcgc gcaaacaaac cgcgcaatcc gtctgacggc ttcctccagg
      961 tcgtccatgc tgcgggtata agcgaagcgc acgcagcgcg cggtgccgtt ggcgccgaag
     1021 tcgacccccg gggtggccgc caccgcctcc ttttccagca gctggcgcgc gaacgcgcgg
     1081 gcgtcgccgc cgcagtcggc gtagatgtag aacgcaccgt gcggctcggc cgggatgctc
     1141 aggccggagc gcttcagcgc cggcacgagg aagtcgcggc gccgcgcgaa ctcgcctctt
     1201 ctcgcctcca gtatcgaaat gctttccggc aggaaggccg ccagggcggc gtgctgcgcg
     1261 gcggacggcg ccgagatgaa aaggtgctgc gccagcttct cgaagccgcg caccgcggtc
     1321 tcgggcagca ccacccagcc cagccgccag ccggtcatgc agaagtactt cgagaagctg
     1381 ttgatcagca ccacctcgcc gggcagcccg gcggcgctgc gtggctcgct gccgtagacc
     1441 aggccctggt agatctcgtc gaccagcagg accccgccgc gcccggcgat gaagtcggcg
     1501 atgcctttca gctggttttc gtcgatcaac gtgccggtcg ggttggacgg cgatccgagg
     1561 agcaggcctt tggtcttcgg gccccacgcg gcgcgcacca tctcgagcgt cggctggaag
     1621 ttgttctgcg ccgacaccgg cagcgttttc gccacgccct cgaaggcgcg cacgaaatga
     1681 cggtagccgg gatagccggg gtcgggcacc aggatctcgt cgcctgcatc tacatagagc
     1741 gctgccgcgg tcagcagccc gccggacgca cctgaggtga tcgcgatcct ttccgggggt
     1801 atcttttttg aaaaacgcgt ctggtagtga gccgcaatag cctcgcgcag cgccggcagg
     1861 ccgagcgtcg cggtgtaggc ggtcaggccg tcgcggatcg cgcgtatgcc ggcctccacc
     1921 acgggcgccg gcgcggtgaa atcgggctcg ccgatctcca tgtgcaccac gtggcgcccc
     1981 gccgcctcga gcgcgtgcgc gcgcgacagc acgtccatca cctcgaaggg ctctatttcg
     2041 tcggcgcggc gcgccgtggg ggtcggcatg agggtgaggg attataattt tttggtgga
//
LOCUS       DASYJA010000391         4344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_694167, whole genome shotgun sequence.
ACCESSION   DASYJA010000391 DASYJA010000000
VERSION     DASYJA010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694167"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /gene="purD"
                     /locus_tag="VGO02_08635"
     CDS             <1..1216
                     /gene="purD"
                     /locus_tag="VGO02_08635"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003272623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEV7477786.1"
                     /translation="AQSPRVQKVYVAPGNAGTATEDGLENLDLTQIADLVAFAEKNDI
                     HLTVVGPEAPLSAGVVDAFRAAGLKIFGPTRAAAQLEASKDFAKSFMVRHKLPTALHR
                     TFDNADEAKAFVARRGAPIVVKADGLAAGKGVVVAMSVAEATAAIDAMLTQKSFGAAG
                     ARVVIEEFLEGEEASFIVMCDGTHILPLATSQDHKRLKDGDQGPNTGGMGAYSPAPVV
                     TPKIHSRVMREIIQPAVQGMASDGVPYTGFLYAGLMIDQAGNPKTLEFNCRMGDPEAQ
                     PIILRLKSDLLDLVEHAVNGKLNEIEADWDRRAALGIVLAAHGYPEEPRKGDRIEGLP
                     KPAEDCRVFHAGTRLEGRNALTNGGRVLCVTALGHSVRSAATRAYEALEGIRFDGMQY
                     RRDIGHRAMTRK"
     gene            1213..2073
                     /gene="hemF"
                     /locus_tag="VGO02_08640"
     CDS             1213..2073
                     /gene="hemF"
                     /locus_tag="VGO02_08640"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEV7477787.1"
                     /translation="MKVLEYFTDLQASIVDALEALDGNPFTTDTWKRPEGGGGTTRIL
                     EGGNLFERGGVNLSHVKGSQLPPSATASRPQLAGRPWEAMGVSLVLHPRNPYCPTVHL
                     NVRYFSAGDTWWFGGGMDLTPYYGFEEDARHFHATCKKASGEHYPRFKKWCDEYFHIK
                     HRNEPRGIGGIFFDDFSEQNFDASFSLTRSVGDHFITAYAPIIERRKAMSYGEKERAF
                     QAYRRGRYVEFNLVYDRGTLFGLQSGGRTESILVSLPPAAAWRYDWRPEPGSPEARLY
                     ADFLRPRDWV"
     gene            2078..2560
                     /gene="msrB"
                     /locus_tag="VGO02_08645"
     CDS             2078..2560
                     /gene="msrB"
                     /locus_tag="VGO02_08645"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV7477788.1"
                     /translation="MKVHVYDSQGELVGPVESSKVVKSDAEWQSQLTPEQYEVARGKG
                     TEAPFCGLLLDNKKDGVYACVCCSLPLFSSGSKFNSDTGWPSFFQPVAPQNVVEEVDD
                     SYGMRRSEILCARCDCHLGHVFEDGPRPTGLRYCVNSESLMFVEREDLAELADPAAAG
                     "
     gene            2599..3156
                     /locus_tag="VGO02_08650"
     CDS             2599..3156
                     /locus_tag="VGO02_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477789.1"
                     /translation="MRLGHAVRALLCCALAACGAQEPVTDADKALFLRVADLAEFGVR
                     YPNAEAAESFSKAKQVDGSYELTYKFETSGDVRPLFIHSSVNVGRDTSEAALVESAEK
                     VGLLIAFKKSGVEERAVPGVRAGKLTLLVKGDVPIGNVFTVRDGKKTHLLILSGLYVR
                     DPAVWQKLMAPKLEQLSNYSPGPKS"
     gene            complement(3153..>4344)
                     /locus_tag="VGO02_08655"
     CDS             complement(3153..>4344)
                     /locus_tag="VGO02_08655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HEV7477790.1"
                     /translation="KALAWLLAVFAAAVALVILGRVDAGYALFVYPPYRVEMSMLLFV
                     LALLFAFLLLYAVFRALSHALALPAHVRAFRARRRRERAHAALAAALQAYYEGRYVRA
                     EKEAAEAFEGGQATGVAALLAARAAHQMRDFERRDRWLERAEGAGESTQAARLVSRAE
                     LALEERDYGAARDALRSLHGAGPKHIATSRMLLRAERGSGDWDEVLRLSTQLAKRDAI
                     APALAEEYKVQATVELLRRASADASTLERRWRSVPDADRTQPRIAAAAAQQAAKLGRA
                     ALARQIVEAALDAEWSPQLVALYGQLPERLDGEALKAEARARIERGERWLIQHERDAQ
                     LLATLGRLCAQAELWGKARSFLEASLSFEEGRAAHLDLARLTERIGQPADAQRHYRRA
                     AELP"
BASE COUNT          723 a         1512 c         1474 g          635 t
ORIGIN      
        1 cgcgcagagc ccgcgcgtgc agaaggtcta cgtcgcgccg ggcaacgccg gcaccgcgac
       61 cgaggacggc ctggagaacc tcgatctgac gcagatcgcc gacctggtgg ccttcgcgga
      121 aaaaaacgac atccacctca ccgtggtcgg ccccgaggcg ccgctttcgg caggcgtggt
      181 ggatgctttc cgggcggccg gcctgaagat cttcgggccg acgcgcgccg ccgcgcagct
      241 cgaggcttcc aaggacttcg ccaagtcctt catggtgcgc cacaagctgc cgaccgcgct
      301 gcaccgcacg ttcgacaacg ccgacgaagc gaaagcgttc gtcgccaggc gcggtgcgcc
      361 gatcgtggtc aaggccgacg gcctcgccgc cggcaagggc gtggtcgtgg cgatgagcgt
      421 cgcggaggcg acggccgcga tcgacgccat gctcacgcag aagagcttcg gcgccgcggg
      481 cgcgcgcgtc gtgatcgagg aattcctgga aggcgaggaa gcgagcttca tcgtcatgtg
      541 cgacggcacg cacatcctgc cgcttgccac cagccaggac cacaagcgcc tgaaggacgg
      601 cgaccagggg ccgaacacgg gcggcatggg cgcctactcg ccggcgccgg tggtgacgcc
      661 gaagatccat tcgcgcgtca tgcgcgagat catccagccc gcggtgcagg gaatggcgag
      721 cgacggtgtg ccgtataccg gcttcctcta cgccgggctg atgatcgacc aggccggcaa
      781 cccgaagacg ctggagttca actgccgcat gggcgatccc gaggcgcagc cgatcatcct
      841 gcgcctcaag tcggacctgc tggacctggt cgagcacgcg gtaaatggaa aactgaatga
      901 aatagaggcc gactgggatc gacgcgccgc cctcggtatc gtgctcgcag cgcacggcta
      961 tccggaggag ccgcgcaaag gcgaccgcat cgagggcctg cccaagccag ccgaggactg
     1021 ccgcgtgttc catgccggca cgaggctcga gggcaggaac gcgctcacca acggcggccg
     1081 cgtgctgtgc gtgaccgcgc tcggccattc ggtgcgcagc gcagccacgc gcgcctacga
     1141 ggcgctcgaa ggtatccgct tcgacggcat gcagtaccgc agggacatcg gccaccgggc
     1201 aatgacgcgc aagtgaaggt ccttgaatac ttcacagacc tgcaagcgag catcgtcgac
     1261 gctctagagg ctctcgacgg gaacccgttc acaacagaca cctggaagcg cccggagggc
     1321 ggcggcggaa caacccgcat tctcgaggga ggcaacctct tcgagcgcgg cggggtcaac
     1381 ctttcccacg tcaaaggcag ccaactccct ccctcggcga ccgcctcgcg cccgcagctc
     1441 gccggcaggc cctgggaagc gatgggcgtc tcgctggtgc ttcacccgcg caacccttac
     1501 tgccccacgg tccacctcaa cgtgcgctat ttctccgccg gcgacacctg gtggttcggc
     1561 ggcggcatgg acctcacgcc gtactacggc ttcgaggagg atgcgcggca ttttcatgcg
     1621 acgtgcaaga aggcgtccgg cgagcactac cctcgcttca agaagtggtg cgacgagtac
     1681 ttccatatca agcaccgcaa cgagccgcgc ggcatcggcg gcatcttctt cgacgatttt
     1741 tcagagcaga attttgatgc ttcgttttct ctcacgcgct cggtcggtga ccacttcatc
     1801 accgcctatg caccgatcat cgagcgccgg aaagcaatgt cgtacggaga aaaagaaagg
     1861 gccttccagg cctaccgccg cggccgctac gtcgagttca acctggtcta cgaccgcggc
     1921 acccttttcg gcctgcagtc gggcgggcgc accgaatcga tcctggtgtc gctgccgccc
     1981 gctgccgcct ggcgctatga ctggcggccc gagcccggct cgcccgaggc caggctctat
     2041 gccgattttc tccggccgcg cgactgggtc tgacgccatg aaagtccacg tctatgactc
     2101 gcagggcgag ctggtcggcc cggtggaaag ctcgaaggtg gtgaagagcg acgccgagtg
     2161 gcagagccag ctcacgcccg agcaatacga ggtggcgcgc ggcaaaggta ccgaggcgcc
     2221 gttttgcggc ctgctcctcg acaacaagaa ggacggcgtc tacgcctgcg tgtgctgctc
     2281 gctgccgctt ttttcctccg gctccaagtt caactcggac acgggctggc cgagcttctt
     2341 ccagccggtc gcgccgcaga acgtggtcga ggaagtcgac gacagctacg gcatgcgccg
     2401 cagcgaaatc ctctgcgcgc gctgcgactg ccacctcggc cacgtcttcg aggacggccc
     2461 tcgccccacc ggcctgcgct actgcgtcaa ctccgaatcg ctgatgttcg tcgagcgcga
     2521 ggacctcgcc gagctggccg atccggccgc ggccggctag ccgtactaca ctagcgaccc
     2581 cgcaaagaag gagctctcat gaggttggga cacgcagttc gcgcgctgct gtgctgcgcg
     2641 ctcgccgcct gcggcgcgca ggagccggtc accgatgccg acaaggcgct gtttctgcgg
     2701 gtcgccgacc tcgccgagtt cggcgtgcgc tatccgaacg ccgaggccgc ggagagcttc
     2761 tcgaaggcga agcaggtgga cggctcgtac gagctcacct acaagttcga gacctcgggc
     2821 gacgtgcggc ccttgttcat ccacagcagc gtgaacgtcg ggcgcgacac ctcggaggcc
     2881 gcgctggtcg agagcgcgga aaaagtcggc ctgctgatcg cattcaagaa aagcggcgtc
     2941 gaggagcgcg cagtacccgg cgtgcgcgcc ggcaagctga ccctgctggt caagggcgac
     3001 gtgccgatcg gcaacgtgtt taccgtgcgc gacggcaaga agacgcacct gctcatcctc
     3061 tccggcctgt acgtgaggga cccggcggtc tggcagaagc tgatggcgcc gaagctcgag
     3121 cagctgtcga actactcgcc ggggccgaag agctagggga gttcggccgc gcgccggtag
     3181 tgacgctggg cgtcggcggg ctggccgatg cgctcggtga ggcgcgcgag gtcgaggtgc
     3241 gccgccctgc cctcctcgaa cgaaaggctc gcctcgagga aactgcgcgc ctttccccac
     3301 agctcggcct gcgcgcacag gcgccccagc gtggcgagaa gctgcgcgtc gcgctcgtgc
     3361 tggatgagcc agcgctcgcc gcgctcgatg cgcgcacgtg cctccgcctt cagcgcctcg
     3421 ccgtccagcc gctcgggcag ctggccgtac agagcgacca gctggggcga ccactccgcg
     3481 tcgagcgccg cttcgacgat ctgccgcgcc agcgccgccc ttcccagctt ggcggcctgc
     3541 tgcgccgccg ccgccgcgat gcgcggctgc gtgcggtcgg catccggaac gctgcgccag
     3601 cgccgctcca gggtcgaggc gtccgccgag gcccggcgca gcagctccac cgtcgcctgg
     3661 accttgtatt cctcggccag cgccggcgcg atggcgtcgc gcttggcgag ctgggtcgac
     3721 aggcgcagca cctcgtccca gtcgccgctg ccgcgctcgg cgcgcagcag catgcggctg
     3781 gtggcgatgt gcttgggtcc ggcgccgtgc aggctgcgca gcgcgtcgcg cgcggcgccg
     3841 taatcgcgct cctccaatgc cagctcggcg cggctcacca ggcgcgccgc ctgcgtgctc
     3901 tcgccggcgc cctcggcgcg ctcgagccag cggtcgcggc gctcgaagtc gcgcatctgg
     3961 tgggcagcgc gcgccgcgag cagcgccgca acgcctgtgg cctgcccgcc ttcgaaggcc
     4021 tcggccgctt ccttctcggc gcgcacgtag cgcccctcgt agtaggcctg cagggctgcg
     4081 gcgagcgcgg cgtgcgcgcg ctcgcggcgg cgccgagcgc ggaatgcacg cacgtgcgcc
     4141 ggcagtgcca gcgcgtgcga cagggcgcgg aacaccgcgt acaggaggag aaacgcgaac
     4201 agcagggcga ggacgaacaa cagcatcgac atctcgacgc gatagggcgg atagacgaaa
     4261 agcgcgtacc cggcatcgac gcgcccgagt atcaccaggg cgacggccgc cgcgaacacc
     4321 gcgagcagcc aggcgagggc cttc
//
LOCUS       DASYJA010000392         1740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1328758, whole genome shotgun sequence.
ACCESSION   DASYJA010000392 DASYJA010000000
VERSION     DASYJA010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328758"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VGO02_08660"
     CDS             <1..196
                     /locus_tag="VGO02_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477791.1"
                     /translation="YLFELEVEGLPAEQRWSLLHVKGRELARSAALFLRESLLYNGAG
                     KEEGNGDPARLQRGSRPDRP"
     gene            147..1640
                     /locus_tag="VGO02_08665"
     CDS             147..1640
                     /locus_tag="VGO02_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEV7477792.1"
                     /translation="MEIPRDYNAAVDLIGRNLAAGRGAKVAYIDDGGTCTYAQLAERV
                     DRAANALRSLGIGREERIAIAMLDSADWVAVFLGAIKAGIVPVALNTLLTPADYEYQI
                     KDSGAKALFVSDPLLKNFGKLERVIPQSQLNDLLQKQKATAQAAPTTRDDICFWLYSS
                     GSTGAPKGTVHLHSHLILTAELYAKPVLGIRESDTVFSAAKLFFAYGLGNAMTFPLAV
                     GATTVLMAERPTPDAVFKRLTEKKPTIFYGVPTLYAALLASPAFPEKAKLSLRVCTSA
                     GEALPAEICKRWTERTGAEVLDGIGSTEMLHIFLSNRPGDVRPGTTGKPVPGYSLRLV
                     DDNGNLVTQPGELGELQISGPTSAIMYWNQREKTKNTFQGPWTRSGDKYSMDEKGYFA
                     YGGRSDDMLKVSGIYVSPVEVEAALITHEAVLECAVVGAEDESKLIKPKAYVVLKQGQ
                     SPSDSLKSALQQHVKDKLAPYKYPRWIEFLNELPKTATGKIQRFKLR"
BASE COUNT          336 a          597 c          528 g          279 t
ORIGIN      
        1 ctaccttttc gagctggaag tcgagggcct gccggccgag cagcgctgga gcctgctgca
       61 cgtgaagggg cgtgaattgg cacgctcggc tgcattattt ctccgcgagt ctctcctcta
      121 caatggcgcc ggcaaggagg agggcaatgg agatcccgcg cgactacaac gcggcagtcg
      181 acctgatcgg ccgtaacctc gccgccgggc gcggcgctaa ggttgcctac atcgacgacg
      241 gcggaacctg cacctatgcg cagctcgccg agcgcgtcga ccgcgccgcc aacgcgctgc
      301 gctcgctcgg catcggccgc gaggaacgaa tcgccatcgc catgctcgac tcggccgact
      361 gggtagcggt gttcctcggt gcgatcaaag cgggcatcgt gcccgtcgcg ctcaacacgc
      421 ttctgactcc ggcggactac gaataccaga tcaaggacag cggcgcgaag gcgctcttcg
      481 tgtccgatcc tctgctgaag aacttcggca agctcgaacg cgtgattccg cagtcccagt
      541 tgaacgacct ccttcaaaag caaaaggcga ctgcgcaagc cgcccccacg acccgcgacg
      601 acatctgctt ctggctctac tcgtcgggct ccaccggcgc gccgaagggc accgtccacc
      661 tgcactcgca cctgatcctc accgccgagc tctacgcgaa gccggtgctc ggcatccgtg
      721 aatcggacac cgtcttctcg gcggcgaagc tcttcttcgc ctacggcctg ggcaacgcca
      781 tgaccttccc gctcgccgtg ggcgcgacga ccgtgctgat ggccgagcgg cccacgccgg
      841 atgcggtctt caagcggctg accgagaaaa agccgacgat tttctacggc gtgccgacgc
      901 tttatgcggc gcttctcgcc agcccggcgt ttcccgagaa agcgaagctg agcctgcgcg
      961 tctgcacctc cgcgggcgag gccctgcccg ccgagatctg caagcgctgg accgagcgca
     1021 cgggcgccga ggtgctcgac ggcatcggct cgaccgagat gctgcacatc ttcctctcca
     1081 atcgacccgg tgatgtgcgc ccagggacca ccggcaagcc ggtgccgggc tattcgttgc
     1141 gcctggtcga cgacaacggc aacctcgtga cccagccagg cgagctcggc gagctgcaga
     1201 tcagcggccc gacctcggcg atcatgtact ggaaccagcg cgagaagacc aagaacacct
     1261 tccagggccc ctggacgcgc agcggcgaca agtactccat ggacgagaaa ggctatttcg
     1321 cctacggcgg ccgctccgac gacatgctca aggtaagcgg catctacgtc tcgccggtcg
     1381 aagtcgaagc cgccctcatc actcacgagg cggtgctcga atgcgccgtg gtcggcgccg
     1441 aggacgagtc gaagctcatc aagcccaagg cgtacgtcgt cttgaaacag ggccagtcgc
     1501 cctcagattc cctgaagagc gccttgcagc agcacgtaaa ggacaagctc gccccctaca
     1561 aatacccgcg atggatcgaa ttcctcaacg agctgccgaa gacggcgacc ggaaagattc
     1621 agaggttcaa gctccggtga aaagcatttc tcgcgtcact cgcgtgatcc ccagcggccc
     1681 ttcctcgcag gtcgacaaca agcagctggt gatcggcgcc gagggctggg agaagacctc
//
LOCUS       DASYJA010000393         1775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_603437, whole genome shotgun sequence.
ACCESSION   DASYJA010000393 DASYJA010000000
VERSION     DASYJA010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603437"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /gene="queG"
                     /locus_tag="VGO02_08670"
     CDS             complement(<1..1009)
                     /gene="queG"
                     /locus_tag="VGO02_08670"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEV7477793.1"
                     /translation="MDYTGLVQRIKAWGAELGFQGVGIAEADLSAAEPRLLDWLSKGW
                     HGEMEYMARHGALRARPEELRPGTLRVISCRMNYAADVSEGISTEPENAYIGRYARGR
                     DYHKVLRDRLQKLCDRIAAEAGPFGYRAFTDSAPVMEVELATRAGLGWRGKHTLLLSR
                     DAGSWFFLGEIYCDLPLPIDQEEKDHCGTCQRCIDICPTQAIRGPYQLDARRCISYLT
                     IEHKSAIPEELRPLIGNRVYGCDDCQVVCPWNGFAQNTSEDDFKSRNGLDRSTLVELF
                     AWTEAEFGERMQGSAIRRIGYERWLRNLAVGLGNAPTSPEVVEALKARENHSSALVKE
                     HV"
     gene            1008..1472
                     /gene="tsaE"
                     /locus_tag="VGO02_08675"
     CDS             1008..1472
                     /gene="tsaE"
                     /locus_tag="VGO02_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV7477794.1"
                     /translation="MSPDLNLPDAAATERLGAALAAGIAPGRVLHLRGDLGAGKTTLV
                     RGLLHTLGHAGRVKSPTYTLVEPYTLSRLDLYHFDFYRFHDKSEWVASGFRDYFSPET
                     ACIVEWPERAGDLLAPPDLRIRLEMAGEGRRALMEACSPSGAAWLSSLPASL"
     gene            1421..>1775
                     /locus_tag="VGO02_08680"
     CDS             1421..>1775
                     /locus_tag="VGO02_08680"
                     /inference="COORDINATES: protein motif:HMM:NF023169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HEV7477795.1"
                     /translation="MQSIRRCLALVLAGFFVGFAGVGAAQAQVLSTRIWPARDYTRLT
                     IESKDEIQYSIFSIKDPARLVLDLEVGELPPALAELHTKVAEDDPYIQGLRTARNRPG
                     VVRLVLDLKTDVKPQA"
BASE COUNT          298 a          579 c          571 g          327 t
ORIGIN      
        1 cgacgtgttc tttcaccaac gcggaagaat ggttttctct tgctttcaat gcttctacca
       61 cctcggggga cgtcggagcg ttcccaagcc ccaccgcgag gttgcgcagc cagcgctcgt
      121 agccgatgcg gcggatggcc gagccctgca tgcgctcgcc gaactctgcc tcggtccagg
      181 cgaacagctc caccagcgtg ctgcggtcaa ggccgttgcg ggacttgaaa tcatcttcgg
      241 aggtattttg tgcgaatccg ttccagggac acacgacctg gcagtcgtcg cagccgtaga
      301 cgcggttgcc gatcagcggc cgcaactcct ccgggatggc gctcttgtgc tcgatcgtca
      361 ggtaggagat gcagcggcgc gcgtcgagct ggtagggccc gcggatcgcc tgcgtcgggc
      421 agatgtcgat gcagcgctga caagttccac aatgatcttt ttcttcttga tcaatgggaa
      481 gaggaagatc gcaatagatc tcgccgagga agaaccagga gccggcgtcg cggctgagga
      541 gcagcgtgtg cttgccgcgc cagcccaggc cggcgcgcgt cgcgagctcg acctccatca
      601 ccggcgccga atcggtgaac gcccggtagc cgaacggccc ggcctcggcg gcgatgcgat
      661 cgcacagctt ctgcagccgg tcgcgcagaa ccttgtgata gtcgcgccca cgcgcatagc
      721 gcccgatata cgcattttca ggttcggtgg agattccctc ggagacgtcg gcggcgtagt
      781 tcatgcggca cgagatcacg cgcaaggtgc ctgggcgcag ttcttcgggc cgcgcgcgga
      841 gcgcgccgtg gcgggccata tactccatct cgccatgcca gcccttcgac agccagtcca
      901 gcaggcgcgg ctcggcggcc gaaaggtcgg cctcggcaat gccgacgccc tggaagccga
      961 gctcagcgcc ccaggccttg atccgctgca cgagtcctgt gtagtccatg tcgcccgatc
     1021 ttaatttgcc ggacgcggcg gccaccgagc ggctgggggc ggccctggcc gccggcatcg
     1081 ctccgggacg ggtcctgcac ctgcgaggcg acctgggtgc cggcaagacc acgctggtcc
     1141 ggggcctcct tcatacgctg ggtcatgcgg gacgggtaaa aagtcctacc tataccctgg
     1201 ttgaacctta tacactttcg agattagact tgtatcattt tgatttctat cgattccatg
     1261 acaagagcga atgggtagcc tcaggtttcc gcgactactt ctccccggag acggcctgca
     1321 tcgtggaatg gccggagcgg gcgggcgacc tgctggcgcc gcccgacctg cggattcgcc
     1381 tcgagatggc cggcgaagga cgccgcgcgt tgatggaggc atgcagtcca tccggcgctg
     1441 cctggctctc gtccttgccg gcttctttgt aggctttgca ggggtagggg ccgctcaggc
     1501 ccaggtactc tccacccgca tctggccggc ccgcgactac acgcgcctca cgatcgagtc
     1561 caaggacgag atccagtaca gcatcttcag catcaaggac cccgcgcgtc tcgtgctcga
     1621 cctcgaagtc ggcgagcttc cgccggccct ggccgagctg catacgaagg ttgccgagga
     1681 cgatccctac attcaggggc tgcgcaccgc gcgcaaccgc cccggcgtgg tgcgcctggt
     1741 gctcgacctg aagaccgatg tgaagccgca ggcct
//
LOCUS       DASYJA010000394         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1328897, whole genome shotgun sequence.
ACCESSION   DASYJA010000394 DASYJA010000000
VERSION     DASYJA010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328897"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VGO02_08685"
     CDS             complement(<1..582)
                     /locus_tag="VGO02_08685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7477796.1"
                     /translation="MAARAPFLPEKVARYIAEHAVREAPVLRELRKATQKVSYSGMQI
                     GADQGNLMALLVKLMGAKRCLEIGTYTGYSALAVALALPKDGTIVCCDISEEWTSVGK
                     PFWKKAGVEDRIDLRIGPALETLQNLKGPFDFVFIDADKPNYARYYERCLELVRPGGL
                     IAIDNVLWSGEVANEKAKDEQTVALRKFNDKLHK"
     gene            complement(582..2105)
                     /gene="ilvA"
                     /locus_tag="VGO02_08690"
     CDS             complement(582..2105)
                     /gene="ilvA"
                     /locus_tag="VGO02_08690"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEV7477797.1"
                     /translation="MKRSDYLQRILKSRVYDVASESPLELAPALSKRIGNRLLLKRED
                     LHPVFSFKLRGAYNKMAGLPAARLKKGVIAASAGNHAQGVALAAQKLGCRALIVMPIT
                     TPRIKIEAVKSRGATVVLHGDSYSDAYAKALKLKRARGLTFIHPYDDPDVIAGQGTIG
                     MEILRQHQKPIDAIFVPVGGGGLISGIAAYVKKINPKIRIIGVEPVDAAAMTESLKKG
                     KRIILEHVGLFADGVAVKQVGEETFKLCRKLVDEMVLADTDEMCAAIKDVFEDTRVVL
                     EPAGALAVAGAKAWIERKNGKKRARGMTLVAICSGANTNFDRLRFIAEEAEVGEHREA
                     ILAVTIPERPGSFKKFCATLGARSVTEFNYRIADSTDAHIFVGVEVQGRKETARLVST
                     LRRHGLKTLDLSDNEMAKWHVRHMVGGRAPAKDELLYRFEFPERPGALVRFLDNMRSD
                     WNISLFHYRNHGADYGRVLVGMQVPRGETGAFRAFLKKLGYPCADETRNPAYRLFLV"
BASE COUNT          327 a          719 c          672 g          412 t
ORIGIN      
        1 cttatgcagc ttatcgttga acttgcgcag cgcgacagtc tgctcgtcct tggccttctc
       61 gttcgccacc tcgcccgacc acagcacgtt gtcgatcgcg atcagtccgc cggggcgcac
      121 gagctccaga caacgctcgt agtacctggc gtagtttggc ttgtccgcat cgatgaagac
      181 gaagtcgaac ggccccttca ggttctgaag ggtctccaag gcagggccga tcctcaagtc
      241 gattctgtct tcgaccccgg ccttcttcca gaagggtttg ccgacgctgg tccattcctc
      301 gctgatgtcg cagcagacga tcgtgccgtc cttcggcagc gcgagcgcca cggcgagcgc
      361 gctgtagccg gtgtaggtgc cgatctcgag gcagcgcttc gcccccatca gcttgaccag
      421 cagcgccatc aggttgccct ggtcggcgcc gatctgcatg ccggaatagg acactttctg
      481 cgtcgccttg cgcagctcgc gcagcaccgg cgcctcgcgc accgcgtgct cggcgatgta
      541 gcgcgcgacc ttttccggca ggaacggcgc gcgagcggcc atcagaccag gaacaggcgg
      601 taggcggggt tgcgcgtctc gtcggcgcag ggatagccga gcttcttcag gaacgcgcgg
      661 aaggcgccgg tctcgccgcg cggcacctgc atgccgacca gcacccggcc gtagtcggcg
      721 ccatggttgc ggtagtggaa caggctgatg ttccagtcgc tcctcatgtt gtcgaggaag
      781 cgcaccagcg cgccggggcg ctcgggaaat tcgaaccggt acagcagctc atccttcgcc
      841 ggtgcgcgcc ctccgaccat gtgcctgacg tgccatttcg ccatctcgtt gtcggagagg
      901 tcgagcgtct tcaggccgtg gcggcgcaag gtgctcacca ggcgcgcggt ctccttgcgc
      961 ccctgcacct cgacgccgac gaagatgtgc gcatcggtcg agtcggcgat gcgatagttg
     1021 aactccgtga cgctgcgtgc gccgagcgtg gcgcagaatt tcttgaagct gccggggcgc
     1081 tccggaatgg tcaccgccag gatcgcctcg cggtgttcgc cgacctcggc ctcctcggcg
     1141 atgaagcgca ggcggtcgaa gttggtgttg gcgcctgagc agattgcgac cagagtcatg
     1201 ccgcgagcgc gctttttccc attttttcgc tcgatccagg ccttggcgcc ggccacggcg
     1261 agggcgccgg ccggctcgag caccacgcgc gtgtcctcga acacgtcctt gatcgccgcg
     1321 cacatctcgt cggtgtcggc gagcaccatc tcgtcgacca gcttgcggca cagcttgaag
     1381 gtctcctcgc cgacctgctt caccgccacg ccgtcggcga acaggccgac atgctcgagg
     1441 atgatccgct tgcccttctt cagcgactcg gtcatggctg ccgcgtcgac cggttcgaca
     1501 ccgataattc ttatttttgg attgattttc tttacatacg cggcgatgcc cgagatcagc
     1561 ccgccaccgc ccaccggcac gaaaatcgcg tcgatcggct tctgatgctg gcgcaggatc
     1621 tccatgccga tcgtgccctg gccggcgatc acgtccgggt cgtcgtaggg atggatgaag
     1681 gtcaggccgc gcgcccgctt cagcttcagc gccttcgcgt aggcatccga ataagagtcg
     1741 ccgtgaagca cgaccgttgc gccccgcgac ttcaccgctt ctatctttat tcggggcgtg
     1801 gtgatcggca tgacgatcag cgcgcggcag ccgagcttct gcgccgccag cgccacgccc
     1861 tgcgcgtggt tgccggcgct cgccgcgatc acgcccttct tcaggcgcgc ggccggcagc
     1921 cccgccatct tgttgtaggc gccgcgcagc ttgaaggaga acaccgggtg cagatcctcg
     1981 cgcttgagca gcagccggtt gccgatccgc ttcgagagcg ccggcgcgag ctcgagcggc
     2041 gactcgctcg ccacgtcgta gacgcgcgac ttcaggatcc gctggagata atcgctgcgc
     2101 ttcatcgggc cgctatactg ccatgatgac
//
LOCUS       DASYJA010000395         6734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2055673, whole genome shotgun sequence.
ACCESSION   DASYJA010000395 DASYJA010000000
VERSION     DASYJA010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6734
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2055673"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..279)
                     /locus_tag="VGO02_08695"
     CDS             complement(112..279)
                     /locus_tag="VGO02_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477798.1"
                     /translation="MKTYPTLAELYSLELQARRMQQREIGRLLRKGAQAVKAALANAV
                     SHFNAKGVRHA"
     gene            394..1332
                     /gene="gcvA"
                     /locus_tag="VGO02_08700"
     CDS             394..1332
                     /gene="gcvA"
                     /locus_tag="VGO02_08700"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HEV7477799.1"
                     /translation="MIYRLPPLNALRVFEAAARHLSFKEAAAELHITQAAVSHQIKSL
                     EEYLGVDLFRRAGRGVQLTEAAKACLPRLREGFDALAAAVELIRERGGEAELTITSPP
                     VFTARWLMPRLADFAKKEPKIDVRVVANSRMVDSGALDSPVFIGTDPRSDASGVEIHL
                     GVGEYPGHRADRLFGVKMVLVASPQLIDGNPPLREPADLAHHVLLHDDAMELVAHGSA
                     WQKWLEAAGVADRVDGSRGPRFSSNILSLEAASQKLGVALALRPLIDADVASGRLRKP
                     FDIEVNPRSAYYLVCPEVIAERPAVKAFRSWLLEQV"
     gene            1341..1610
                     /gene="infA"
                     /locus_tag="VGO02_08705"
     CDS             1341..1610
                     /gene="infA"
                     /locus_tag="VGO02_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV7477800.1"
                     /translation="MAKEEMLEMEGTVQEVLPNTQFRVALENGAIVLAYAGGKVRQHR
                     IRILAGDRVRLEISPYDITRGRINYRHKDENPVRMAPRRPTYKKH"
     gene            1704..2105
                     /locus_tag="VGO02_08710"
     CDS             1704..2105
                     /locus_tag="VGO02_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEV7477801.1"
                     /translation="MRSRDVSSWMWSEALRLLEQADRLHRQFFLTREPASWEPPLDVV
                     ESGDELRLQLALPGVSPDAVTVVTDADGVVVSAIRGFACRSREARIHRVEIPYGRFER
                     RVELPFDQLELAGKTLQDGILTLTFRRKEGA"
     gene            2102..4507
                     /gene="lon"
                     /locus_tag="VGO02_08715"
     CDS             2102..4507
                     /gene="lon"
                     /locus_tag="VGO02_08715"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEV7477802.1"
                     /translation="MNQDVLAKDDNAERNAAATPGGRGAALKPIPDDALILIPMRNTV
                     LFPGVISPITVGRKVSVAAAQEAVRSEKRIGFLLQKDAQKNDVRPDDLYWVGTSGTVV
                     RYITGAEGAHHLVVQGQERFRVLEFLQGWPFLVARVALVTDASEMTPEIEARFLQLKQ
                     QAAEAIGLLTNAPEELGTLVQGVASPSVLADMVANLLDVKNEEKQALLETFELKTRLD
                     KVLALLGERVGVLRISKEIGDRTKKEFDERQREHVLREQMRQIQKELGEGEDSAAELD
                     ELKKAIDDAGMPEDTQKYARKEFKRLQRMGEGSAEGSMLRTYLEWLAELPWKDSSTQE
                     IDVAKARQVLDEDHFGLDKIKRRILEHLAVRKLNPNGKSPILCFVGPPGVGKTSLGQS
                     IARATGRKFQRISLGGVHDEAEIRGHRRTYIGALPGAIIQAIRRAESRTPVLMFDEID
                     KVGAGGFHGDPSSALLEVLDPEQNAKFRDNYLGVDFDLSKVMFITTANMLDTIPGPLR
                     DRMEIIALPGYTTEEKMEIANRYLVKRQLEANGLSSSQVELREDAIRAIIEDYTREAG
                     VRTLERDIGAVLRNVAMKVAEGASSKIEIRAEDLHAVLGSRKFENETAQRTAVPGVAT
                     GLAWTPVGGDILFIEASKVPGSGKLILTGQLGDVMKESAQAALTLAKGYTGEILDKVD
                     IHVHVPAGATPKDGPSAGVAMFLALTSLLYNKPVRADVAMTGEISLRGLVLPIGGVKE
                     KTLAALRAGIRTVMLPRRNEKDLEDVPAEARAKLEFVFLDRVEDAFRTAIGELPAPEK
                     KAA"
     gene            complement(4458..4646)
                     /locus_tag="VGO02_08720"
     CDS             complement(4458..4646)
                     /locus_tag="VGO02_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477803.1"
                     /translation="MSKQEPEFRHPPIWIHASEAGKWLLSRREAKRRAVLAILDRVPD
                     AATKPLSSRAPAARRSRS"
     gene            4830..6218
                     /locus_tag="VGO02_08725"
     CDS             4830..6218
                     /locus_tag="VGO02_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477804.1"
                     /translation="MNRIRSWRRLLAIGIALCVSGCAAVRSYDHELGGTLDRAALGNV
                     DGAIKLLDDANADKRNLLYQLELGMLQRLGSRYDESQKAWTSASAAIEGSRSAGVLDL
                     AKMAGGAASYVLSDKLRPYEAHDYEQVMLLTYMALNHLAMGDYDGARVAIKQTHELEA
                     EVAERRARQLAAVEEEAKKRGARSSFKDLNGYPVATIDNPAVNALRNGYQSALSHYLA
                     GFIYEALGEPSLAAPGYRLANELQPDQPLLEEALAGLDARTAAPDDGMTDVLLIVGSG
                     SAPALQSRQFTTPILINNKLIFIPAAYPVMTPVEAARPLQLVVDGRALPLAPLTSIDL
                     MARRSLQDDMPSIMLRATIRSTTSAVLQYQAQNQRGDRDGAGVALAAGLLAIGAVMLN
                     SADDRTWRALPSDVAIARTRLPPGQHKVLLQTLEGEQAVPIGVSGRHAVVDFRLFRRQ
                     VFVSAPKAGVAR"
     gene            6241..6675
                     /locus_tag="VGO02_08730"
     CDS             6241..6675
                     /locus_tag="VGO02_08730"
                     /inference="COORDINATES: protein motif:HMM:NF018889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HEV7477805.1"
                     /translation="MNAKWIVTAAALGLLAAGCATKKGFEVPGFAFGCPVGETKVGSM
                     DDLVKAKVVSEGRRSTVQATEVRCSRTGDLLKIDANLNNDSKSVKRVSYKFRWIDREG
                     MRAWEEESWKPIMLYDNSNLMVNTVSPTNKAVDFRLILRSDE"
BASE COUNT         1190 a         2267 c         2251 g         1026 t
ORIGIN      
        1 ccgaagccga gttcccagcg ttccgcagcg tgcatctgca gcgagtagcg ctgctgcacc
       61 tgcttgggca gggaatcgag cgcatctttc cagaatggcg tgacgaattc gttacgcatg
      121 gcgaaccccc ttggcgttga aatgcgaaac tgcattcgca agcgccgcct tgactgcctg
      181 cgctcctttt cggagcagcc ggccgatctc gcgctgctgc atgcggcggg cctgcagctc
      241 gagtgagtac aattcggcaa gggtcggata cgtcttcatt gcctttcctt tcgtgcctcc
      301 ttctcttttc atgcctgcac aagataaggc cctgtaccgc aagggacaaa cgatgaattc
      361 tcattcctcg cataagaaaa actcattgaa agcgtgatct atcgcctgcc tccgctgaat
      421 gcattgcgcg tgttcgaggc cgccgcgcgc catctgtctt tcaaggaagc cgccgccgag
      481 ctgcacatca cgcaggccgc ggtcagccac cagatcaaga gcctcgagga gtacctcggc
      541 gtggacctgt ttcgccgcgc cgggcgcggc gtgcagctca ccgaggcggc gaaagcctgc
      601 ctgccgcggc tgcgcgaggg cttcgacgcg ctcgctgcgg cggtcgagct gatccgcgag
      661 cgcggcggcg aagccgagct gaccatcacc tcgccgccgg tgttcaccgc gcgctggctg
      721 atgccgcgcc tggccgactt cgcgaagaaa gagccgaaga tcgacgtgcg cgtggtggcc
      781 aacagccgca tggtcgactc cggcgcgctc gattcgccgg tgttcatcgg caccgacccg
      841 cgcagcgacg cctcgggggt cgagatccac ctcggcgtgg gcgagtatcc cggccatcgc
      901 gccgaccgcc tgttcggcgt gaagatggtg ctggtggcga gcccgcagct gatcgacggc
      961 aatccgccgc tgcgcgagcc ggccgacctt gcccaccacg tcctgctgca cgacgacgcg
     1021 atggagctgg tggcgcacgg cagcgcctgg cagaagtggc tcgaggcggc gggggtcgcc
     1081 gaccgcgtcg acggcagccg cgggccgcgc ttctcgtcca acatcctgtc gctcgaggcg
     1141 gcgtcgcaga agctcggcgt cgcgctcgcg ctgcgcccgc tgatcgacgc cgacgtggcc
     1201 tcgggacgcc tgcgaaaacc cttcgacatc gaggtcaatc cgcgctccgc ctattacctc
     1261 gtctgccccg aggtcatcgc cgagcgcccc gcggtcaagg cgttccggtc ctggctgctc
     1321 gagcaggtat aatccttcgc atggccaagg aagagatgct cgagatggaa ggcacggtgc
     1381 aggaagtgct tcccaatacg cagttccgcg tcgcgctgga aaacggcgcg atcgtgcttg
     1441 cctacgccgg gggcaaggtg cgccaacacc gcatccgcat cctcgccggc gaccgcgtcc
     1501 gcctggagat ctcgccgtac gacatcaccc gcggccgcat caactaccgg cacaaggacg
     1561 agaaccccgt ccgcatggcg ccgcgccgcc cgacctacaa aaagcattga atctctgtag
     1621 ttgaagtaaa tccctacgct ccgtagggat cgctgcttgc aagaggcttg attcggcggc
     1681 ttggcgtcgc cagataagcc cttgtgagat cccgcgacgt ttcaagctgg atgtggagcg
     1741 aggcgctgcg tctgctcgag caggccgacc ggctgcatcg ccagttcttc ctcacccgcg
     1801 agcccgcctc gtgggagccg ccgctcgacg tggtcgagag cggcgacgag ctgcgcctgc
     1861 agctcgccct tcccggcgtc tcgcccgacg cggtgaccgt agtcaccgac gcggacgggg
     1921 tcgtcgtttc cgcgatccgc ggcttcgcct gccgcagccg cgaagcgcgc atccaccgcg
     1981 tcgaaatccc ctacggccgc ttcgaaaggc gggtcgagct gcccttcgac cagctggagc
     2041 tcgccggaaa aaccctacaa gacggcattc ttactttgac gttccgcagg aaggaaggcg
     2101 catgaaccag gacgtgctgg caaaagacga caacgctgag cgcaacgccg cggcgacacc
     2161 cggcggccgc ggcgcggccc tcaagccgat ccccgacgac gcgctgatcc tcatcccgat
     2221 gcgcaacacg gtgctgttcc ccggcgtgat ctcgccgatc acggtcgggc gcaaagtgtc
     2281 ggtggccgcc gcgcaggagg cggtgcgcag cgagaagcgc atcggcttcc tgctgcagaa
     2341 ggacgcgcag aagaacgacg tgcggcccga cgacctgtac tgggtcggca cctccggcac
     2401 ggtcgtgcgc tacatcaccg gcgccgaggg cgcgcaccac ctcgtggtgc agggccagga
     2461 gcgcttccgc gtgctcgagt tcctgcaggg ctggccgttc ctcgtcgcgc gcgtcgcgct
     2521 ggtgaccgac gccagcgaga tgacccccga gatcgaggcg cgcttcctgc agctcaagca
     2581 gcaggcggcc gaggcgatcg gcctgctcac caacgcgccc gaggagctcg gcacgctggt
     2641 gcagggcgtc gcctcgccct cggtgctcgc cgacatggtg gccaacctgc tcgacgtgaa
     2701 gaacgaggag aagcaggcgc tgctcgagac cttcgagctc aagacgcggc tcgacaaggt
     2761 gctcgcgctg ctcggcgagc gcgtcggcgt gctgcgcatc tccaaggaga tcggcgaccg
     2821 gacgaagaag gagttcgacg agcgccagcg cgagcacgtg ctgcgcgagc agatgcgcca
     2881 gatccagaag gagctgggcg agggcgagga ttcggccgcg gagctcgacg agctgaagaa
     2941 ggccatcgac gacgccggca tgcccgagga cacgcagaag tacgcgcgca aggagttcaa
     3001 gcgcctgcag cgcatgggcg agggcagcgc cgagggctcc atgctcagga cgtacctgga
     3061 atggcttgcc gagcttccat ggaaagactc ttcaacccag gagatcgacg ttgccaaagc
     3121 acgtcaggtt cttgatgaag accatttcgg cctcgacaag atcaagcggc gcatcctcga
     3181 gcacctcgcg gtgaggaagc tcaatccgaa cggcaagagc ccgatcctgt gcttcgtcgg
     3241 gccgccggga gtcggcaaga cctcgctcgg ccagtcgatc gcgcgcgcca ccggacgcaa
     3301 gttccagcgc atctcgctcg gcggcgtgca cgacgaggcc gagatccgcg gccaccggcg
     3361 cacctacatc ggcgcgctgc cgggcgccat catccaggcg atccgccgtg ccgagtcgcg
     3421 cacgccggtg ctgatgttcg acgagatcga caaggtcggc gcgggcggct tccacggcga
     3481 cccgtccagc gccttgctcg aagtactgga cccggagcag aacgcgaaat tccgcgacaa
     3541 ctacctgggc gtcgacttcg acctgtcgaa ggtgatgttc atcaccaccg ccaacatgct
     3601 cgacacgatc cccgggccgc tgcgcgaccg catggagatc atcgcgctcc ccggctatac
     3661 gaccgaggag aagatggaga tcgccaaccg gtaccttgtg aagcggcagc tcgaagccaa
     3721 cggcctttcc tcttcccagg tagaactgcg tgaagatgcg ataagggcaa tcatcgagga
     3781 ctacacgcgc gaagcgggcg tgcgcacgct cgagcgcgac atcggcgcgg tgctgagaaa
     3841 tgtcgccatg aaggtcgccg aaggcgcctc ctcgaagata gaaatccgtg cagaggactt
     3901 gcacgcagtc ctgggttcga ggaaattcga gaatgaaacc gcgcaaagaa ccgcggtgcc
     3961 cggcgtcgcc accggcctcg cctggacgcc ggtgggcggc gacatcctgt tcatcgaggc
     4021 gtccaaggtg cccggctcgg gcaagctgat cctcaccggc cagctcggcg acgtgatgaa
     4081 ggagtcggcg caggccgcgc tgacgctggc gaaaggctac accggcgaga tcctcgacaa
     4141 ggtcgacatc cacgtccacg tgccggccgg cgcgacgccg aaggacgggc cgtcggcggg
     4201 cgtggcgatg ttcctggctc tgacttccct gctctacaac aagccagtca gggccgacgt
     4261 ggccatgacc ggcgagatct cgctgcgcgg gctggtgctg ccgatcggcg gggtgaagga
     4321 gaagacgctc gccgcgctgc gcgccggcat caggacggtg atgctgccgc ggcgcaacga
     4381 gaaggacctc gaggacgtgc cggccgaggc acgggcgaag ctggagttcg tgttcctcga
     4441 ccgcgtcgag gacgcgttca ggaccgcgat cggcgagctg ccggcgcccg agaagaaagc
     4501 ggcttagtcg cggcgtccgg cacgcggtcc aggatggcga ggaccgcgcg ccgcttcgcc
     4561 tcgcggcgcg agagcagcca cttgcccgcc tcggaggcgt ggatccagat cggcgggtgg
     4621 cggaactcgg gctcttgctt cgacatgcgg ggagaagcct acgttatccc gggatggttt
     4681 tccatctccc ggcgtccgac atgaaaacat ccgccgcttc tgctgcgctg cgatcaccat
     4741 aaaatcaagc gcctgcctcg gattgcattt ttttccgtga gccttgcggc gtaaaatggc
     4801 gctcgcaata gaaatatcgc aggggaggta tgaaccggat tcgcagctgg cgccgcctgt
     4861 tggccatcgg catcgcgctg tgcgtctccg gctgcgccgc cgtgcgcagc tacgaccacg
     4921 agctcggcgg caccctggac cgggcggcgc tcggcaacgt cgatggcgcg atcaagctgc
     4981 tcgacgacgc caacgccgac aagaggaacc tgctctacca gctcgagctc ggcatgctgc
     5041 agaggctcgg cagccgctac gacgagagcc agaaggcctg gacctcggcg agcgccgcca
     5101 tcgagggctc gcgctcggcg ggcgtgctcg atctcgccaa gatggcgggc ggcgccgcga
     5161 gctatgtgct cagcgacaag ctgcggccct acgaggcgca cgactacgag caggtcatgt
     5221 tgctcaccta catggcgctc aatcacctcg cgatgggcga ctacgacggc gcgcgcgtgg
     5281 cgatcaagca gacccacgag ctcgaggccg aggtcgccga gcggcgcgcc cgccagcttg
     5341 cggcggtgga ggaggaagcg aagaagcgcg gcgcgcgctc gagcttcaag gacctgaacg
     5401 gctacccggt ggcgaccatc gacaaccccg cggtgaacgc gctgaggaac ggctaccaga
     5461 gcgcgctctc gcactacctc gccggcttca tctacgaggc gctcggcgag ccgagcctcg
     5521 ccgcgccggg ctatcgcctg gcgaacgagc tgcagcccga ccagccgctc ctggaagagg
     5581 cgctcgccgg gctcgacgcg cggacggcgg cgcccgacga cggcatgacc gacgtgctgc
     5641 tcatcgtcgg cagcggctcg gcgccggcgc tgcagtcgcg ccagttcacg acgccgatct
     5701 tgatcaacaa caagctgatc ttcattcccg cggcgtatcc ggtcatgacc cccgtggagg
     5761 cggcgcggcc gctgcagctg gtcgtggacg ggcgggcgct gccgctcgcg ccgctcacca
     5821 gcatcgacct catggcgcgc cgcagcctgc aggacgacat gccgtccatc atgctgcgcg
     5881 ccaccatccg cagcaccacc agcgcggtcc tgcagtacca ggcgcagaac cagcgcggcg
     5941 atcgtgacgg cgccggggtc gcgctggcgg ccgggctgct cgccatcggc gcggtgatgc
     6001 tcaattcggc cgacgaccgc acctggcgcg cgctgccctc cgatgtcgcc atcgcgcgca
     6061 ctcgcctgcc gcccgggcag cacaaggtgc tgctgcagac gctggaaggc gagcaggccg
     6121 tgccgatcgg cgtttccggc cggcacgcgg tggtagactt tcgtctgttc cgccgtcagg
     6181 tttttgtaag tgcacccaaa gccggtgtcg cgcgttaaac gctttgggag ggaggtcaag
     6241 atgaatgcga aatggatcgt taccgcggct gccctgggct tgctcgcggc cggctgcgcc
     6301 accaagaagg gcttcgaggt gccgggcttt gccttcggct gcccggtcgg cgagaccaag
     6361 gtgggctcga tggacgacct ggtcaaggcg aaggtggtga gcgagggccg gcgcagcacc
     6421 gtgcaggcca ccgaggtgcg ctgctccagg accggcgacc tgctgaagat cgacgccaac
     6481 ctcaacaacg acagcaagag cgtgaagcgc gtctcctaca agttccgctg gatcgaccgc
     6541 gagggcatgc gcgcctggga agaggagtcc tggaagccga tcatgctcta cgacaactcc
     6601 aacctgatgg tcaacacggt ctcgccgacc aacaaggcgg tcgacttccg cctgatcctg
     6661 aggagcgacg aatgaaagcc acccgaggtt tggcgctcgc cgcggcgctc gccgccgccg
     6721 cgctcgccgg cggc
//
LOCUS       DASYJA010000396         2823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1966052, whole genome shotgun sequence.
ACCESSION   DASYJA010000396 DASYJA010000000
VERSION     DASYJA010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1966052"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="VGO02_08735"
     CDS             complement(<1..1133)
                     /locus_tag="VGO02_08735"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEV7477806.1"
                     /translation="MKPLAAAVLVVAALELFVFHFFRSFENWVLDAFVKHQAAALAPD
                     PEIVLVNIDEKSLVTMEGGSIGAGRFPWPREVYATLIEGLAAQQPRAIVFDIMFSEPD
                     RFRPQSDQAFIETASRHENIYYPMVRLDSKEGARAAEVAPLIGLVRRPGADPEARIAV
                     IPPLVLPPKLWRTGTINFLPDEDSVGRRYHLRQRIGGWELPSLPARVAMDLGYPLPDA
                     DDIVLAWRGRADAFPRISFSDLYEDFEREKRQRPADEFRGKIVLIGANAAGLGDLRVT
                     PLSNTQPGGEILGTAIENLKNDRHMRYAPVWWPAGIGLALLSFLYYAFRRALDVRVTG
                     AVLAAATILLLGLSWFLAGRLVLLPLLTPLAAAWTFYAWAGLS"
     gene            complement(1130..1954)
                     /gene="metF"
                     /locus_tag="VGO02_08740"
     CDS             complement(1130..1954)
                     /gene="metF"
                     /locus_tag="VGO02_08740"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEV7477807.1"
                     /translation="MADNSFEFFPPRTAEGKLRLRDTWQELAKLKPRFFSCTYGAGGS
                     TRDGTLETVMEIRGAGHQAAPHLSCVASTRQDIAEQLERYKASGIRHLVALRGDLPSG
                     VAASGEFRYASELVAFIRETTGDWFHVEVACYPEYHPQTRYAADEVRNFKKKVDAGAN
                     AAITQYFYNVDAYFRFVEDCRAAGISIPIVPGVMPIGNFAQLARFSDACGAEIPRWMR
                     LKLDGYRDDAASIRAFGLDTVTALCEKLIASGAPGLHFYTLNQAALSAEIWRRLRL"
     gene            complement(1944..>2823)
                     /locus_tag="VGO02_08745"
     CDS             complement(1944..>2823)
                     /locus_tag="VGO02_08745"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEV7477808.1"
                     /translation="GYFGARLVQGGADVSFLARGKHLAAIREKGLTIESPQGNVRLKV
                     KVFEDPAEAGQVDAVMFAVKLWDTEAAATQIKPIIGKRTVVIPLQNGVESIDALRKLL
                     PASAVMGGSAYIATRVREPGVIEHTGNMARLQFGALLASQHNAADAFLAACRQAGLNA
                     ELCEDIVRANWEKFVFLVGVSSATAATRKPLGTVRADADMRWLFEQTMRETWHLARKR
                     GVELPDDFVESRLAFADTLHTEMRASLLHDLEAGHRLEAPWLCGAVARMSAQAGLDAP
                     VNRAIFAALKPYVNGR"
BASE COUNT          445 a          942 c         1009 g          427 t
ORIGIN      
        1 tccgacagcc ccgcccaggc gtagaaggtc cacgcggcgg cgaggggagt gagcagcggc
       61 aggagcacca gtcgccccgc caggaaccag ctgagtccga gcagcaggat cgtcgccgcg
      121 gcgagcaccg cgccggtaac ccttacgtcg agggctcgcc tgaaagcgta gtacaggaag
      181 gacagcaggg ccaagccgat tcctgccggc caccacactg gcgcgtagcg catatggcga
      241 tcgttcttca ggttctcgat cgccgtgccg aggatctcgc cgcccggctg ggtgttcgac
      301 agcggcgtca cgcgcaggtc gccgagcccc gccgcgttcg cgccgatgag cacgatcttg
      361 ccgcggaact cgtcggccgg ccgctggcgt ttctcgcgct cgaagtcctc gtacaggtcg
      421 ctgaaggaga tccgcgggaa ggcatcggcc cggccgcgcc aggcgagcac gatgtcgtcg
      481 gcgtccggca gcggataacc gagatccatg gcgacccgcg ccgggagcga cggcagctcc
      541 cagccgccga tacgttgccg caagtggtag cgccggccga cgctgtcctc gtcgggaaga
      601 aagttgatgg ttccggtgcg ccacagcttc ggcggcagga cgagcggcgg gatgaccgcg
      661 atgcgcgcct cggggtcggc gccgggccgg cgcaccaagc cgatgagcgg cgctacctcg
      721 gcggcgcgcg cgccctcctt cgaatcgagg cgcaccatcg ggtagtagat gttctcgtgg
      781 cgcgaggccg tctcgatgaa cgcctggtcg ctctgcggcc ggaaccggtc cggctccgag
      841 aacatgatgt cgaagacgat ggcccgcggc tgctgcgcgg cgagcccctc tatcagggtg
      901 gcgtacacct cgcgcggcca ggggaagcgg ccggcgccga tgcttccccc ctccattgtg
      961 acgaggcttt tctcgtcgat gttgacgagg acgatttcgg gatcgggcgc gagcgccgcc
     1021 gcctggtgct tgacgaaggc gtcgagcacc caattctcga agcttctgaa aaaatggaaa
     1081 acaaacagct ccagcgccgc aacgaccagc accgcggcgg cgagcggctt cacagcctga
     1141 ggcgccgcca gatttccgcg ctgagcgcgg cctggttgag ggtgtagaaa tgcaggcccg
     1201 gcgcgccgga ggcgatgagc ttctcgcaca gcgcggtgac cgtgtcgagg ccgaaggcgc
     1261 ggatcgaggc ggcgtcgtcg cggtagccgt cgagcttgag gcgcatccag cgcgggatct
     1321 cggcgccgca ggcgtcggag aagcgcgcca gctgcgcgaa gttgccgatc ggcatcacgc
     1381 cgggaacgat cgggatcgag atgcctgcgg cgcggcaatc ctcgacgaag cggaaatacg
     1441 cgtccacgtt gtagaaatac tgcgtgatgg ccgcgttggc gccggcgtcg accttcttct
     1501 tgaagttgcg cacttcgtcg gcggcgtagc gcgtctgcgg gtggtactcg gggtagcagg
     1561 ccacctcgac atggaaccag tcgccggtgg tctcgcggat gaacgcgacc agctcggagg
     1621 cgtagcggaa ctcgccggag gcggcgacgc cggagggcag gtcgccgcgc agcgccacca
     1681 ggtgccggat gccgctcgcc ttgtatcgct cgagctgctc ggcgatgtcc tggcgcgtcg
     1741 aggcgacgca ggaaagatgc ggcgcggcct ggtggccggc gccgcggatc tccatcaccg
     1801 tctcgagcgt gccgtcgcgc gtcgagccgc cggcgccgta ggtgcaggag aagaagcgcg
     1861 gcttgagctt ggccagctcc tgccaggtgt cccgcaggcg cagttttcct tccgctgtgc
     1921 gcggcggaaa gaactcgaag ctgttatcgg ccatttacgt agggcttgag ggcggcgaag
     1981 attgcgcggt tgaccggggc gtcgagccca gcctgcgcgc tcatgcgcgc cacggcgccg
     2041 cacagccagg gcgcctcgag gcggtgcccg gcctcgaggt cgtgcaagag agacgcgcgc
     2101 atttccgtgt gcagggtatc cgcgaaggcg aggcggctct cgacgaaatc atccggcagc
     2161 tccacgccgc gcttgcgcgc gaggtgccag gtttcgcgca tcgtctgctc gaacagccag
     2221 cgcatgtccg cgtcggcgcg caccgtgccg agcggcttgc gcgtcgccgc ggtggcgctg
     2281 gagacgccga ccaggaacac gaacttctcc cagttcgctc gcacgatgtc ctcgcacagc
     2341 tcggcgttga gcccggcctg gcggcacgca gcgaggaagg cgtcggctgc gttgtgctgc
     2401 gaggcgagca gcgcgccgaa ctgcagccgc gccatattgc cggtgtgctc gatcacgccc
     2461 ggctcgcgca cgcgggtggc gatgtacgcc gagccgccca tcaccgcgct cgccggcagc
     2521 agcttgcgca gggcgtcgat cgactcgacg ccgttctgca acgggatcac cacggtgcgc
     2581 ttgccgatga tcggcttgat ctgcgtcgcg gcggcttcgg tgtcccacag cttgaccgcg
     2641 aacatcaccg cgtcgacctg gcccgcctcg gccggatcct cgaacacctt gaccttgagc
     2701 cggacgttgc cctgcgggct ctcgatcgtc aggcccttct cgcggatcgc ggcgaggtgc
     2761 ttgccgcgcg caaggaacga cacatcggcg cccccctgga cgaggcgcgc accgaagtag
     2821 ccg
//
LOCUS       DASYJA010000397         1985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_514423, whole genome shotgun sequence.
ACCESSION   DASYJA010000397 DASYJA010000000
VERSION     DASYJA010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514423"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="VGO02_08750"
     CDS             complement(<1..1177)
                     /locus_tag="VGO02_08750"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HEV7477809.1"
                     /translation="MRLDPNAISALASRLSRGSVLVSATNGKTTTAAMAAGILNRASV
                     PLVHNRAGANMAGGIASTLLTASRPRGGIAGELGLFEIDEFWLGMLAAQLQPRVIVLC
                     NLFRDQLDRYGELESIAERWAQAVSESAANGARLVLNADDPMVADLGRTHINAGESRS
                     PEGGPDPRVLYYGVQDDSLALAGMAHAADAKHCRRCGAPYVFDAIYLGHLGHYHCTSC
                     GQTRPVPAVSASAVTLQGVRSARFTLHTPSGEAQVTLALPGLYNVYNALAAAALATAL
                     GIGLQDIVAGLQETKAAFGRAETVVFDGRELQILLIKNPAGANEVLRTLALEDGEHDL
                     LAVLNDKTADGRDVSWIWDADFELLAPRLRRVVCAGTRAADLAARLTYAGVDPSRIEI
                     "
     gene            complement(1279..>1985)
                     /locus_tag="VGO02_08755"
     CDS             complement(1279..>1985)
                     /locus_tag="VGO02_08755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEV7477810.1"
                     /translation="ALSGDGMVGAIADVLREHPDALLGILPGGRGNDLARVLGIPEDP
                     VAACAVIAHGVPRATDLGEVDGHAFVGIASAGFDSEANRIANEAPARLGGLVYAYGAL
                     RALFSWRPANFEIELDPPGRRISFTGYTIAAANSGVYGGGMRLAPDARLDDGLLDIVA
                     LERVGRLQFLVDLPKAFKGTHVRLPYVQVLRAAEVSISADRPFTLYADGDPIAELPVR
                     VRALRGAVQVLVAEER"
BASE COUNT          337 a          666 c          667 g          315 t
ORIGIN      
        1 cgatctcgat gcggcttgga tcgacgccgg cgtacgtcag ccgggcggcg aggtcggccg
       61 cgcgcgtgcc ggcacacaca acgcgccgca gtcgcggcgc cagaagctcg aagtcggcat
      121 cccagatcca ggagacgtcg cggccatcgg cagtcttgtc attgagcacc gcaagcagat
      181 catgctcgcc atcctccaac gcaagcgtgc gcagcacctc gttggcaccg gcggggttct
      241 tgatcagcag gatttgtagc tcgcggccgt cgaacacgac ggtctctgct cggccgaatg
      301 ctgctttggt ttcctgcaag ccggcaacaa tgtcctgcaa tccgattccg agcgccgtgg
      361 ccagggcggc cgcggcgagt gcgttgtaga cgttgtagag accgggcagc gcaagtgtca
      421 cctgcgcttc gccggagggc gtatgcagcg tgaagcgggc ggaacgcacc ccttgcagcg
      481 tcacggcgct cgcgctcact gcggggacgg gacgggtctg cccacagctt gtgcagtggt
      541 agtgccccag atggcccagg tagattgcgt cgaacacgta cggtgctcca caacggcggc
      601 agtgcttggc atcggcggca tgcgccatcc ccgccaaggc caacgagtcg tcctgcacgc
      661 cgtagtagag aacgcgagga tcgggacctc cttcaggact acggctctcg ccggcattta
      721 tgtgcgtgcg gcctagatcc gcgaccattg gatcgtccgc gttcaggacc agccgcgcgc
      781 cgttcgccgc gctctcgctc acggcttgtg cccagcgctc ggcaatgctc tcgagctctc
      841 cgtagcggtc gagctgatca cggaagaggt tgcagagcac gatcacccgt ggctgcagct
      901 gagcggcaag catgcccagc cagaactcgt cgatctcgaa caggccgagc tctccggcga
      961 tgcctccccg cgggcgggag gcggtcagca gcgtcgaggc gatgccaccc gccatgttgg
     1021 ctcctgcccg attgtgcacg agcggcacgc ttgcacggtt gagaatgccg gccgccatcg
     1081 cggcggtagt tgtcttcccg ttcgtggcag agacaagcac gctgccgcgt gacagacggg
     1141 acgccagtgc gctgatggcg ttggggtcga ggcgcatcag aacctttcca ggcgcgcttg
     1201 tcccaccact gccgcgtacg cgcgcgagtg caccgacgag ccgtgccaag gccaacttgg
     1261 gagcgagcgc tccagccatc agcgctcctc ggcgaccaat acctgcacgg ctccgcgcag
     1321 cgcgcgcacc cgcacgggca gctctgcgat tgggtcaccg tcggcgtaca gcgtgaacgg
     1381 cctatctgct gagatcgaga cttccgccgc gcgcagcacc tgcacatagg gcagccgcac
     1441 atgggtgccc ttgaatgcct tgggtaaatc cacgaggaac tgcagtcgtc ccacgcgttc
     1501 aagcgcgacg atgtcgagca ggccgtcatc gaggcgggcg tcgggagcaa ggcgcatacc
     1561 gccaccgtag acgccggagt tggcggcggc gatcgtgtag ccggtgaagc tgatccgtcg
     1621 accgggcggg tccagctcga tctcaaagtt tgccgggcgc cacgagaaca gcgcacgcaa
     1681 cgctccgtag gcgtacacaa ggccgccaag ccgggcgggt gcttcgttgg cgatccggtt
     1741 cgcctcgctg tcaaagccgg cgctggcgat accgacgaac gcatggccgt cgacctcgcc
     1801 gaggtcggtt gcgcgcggaa cgccatgcgc gatcactgcg cacgcagcga ccgggtcctc
     1861 ggggatgccg agcacccggg cgagatcgtt gccgcggccg cctggcagaa tccccaggag
     1921 cgcgtccggg tgctcgcgca gcacgtcggc gatcgcgcca accatgccgt cgccgctgag
     1981 cgcta
//
LOCUS       DASYJA010000398         1857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2329199, whole genome shotgun sequence.
ACCESSION   DASYJA010000398 DASYJA010000000
VERSION     DASYJA010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2329199"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VGO02_08760"
     CDS             <1..517
                     /locus_tag="VGO02_08760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7477811.1"
                     /translation="LPDVALRTFAADPATEMSEAFVEATRAKGDECYAIRDGANLAAY
                     GWYATTPTTAGSPELLLHFGDEYVYMYKGFTDPRYRGQRLHAIGMTRALEEYRRRGYR
                     AMVSYVESTNFDSLKSCFRMGYRVFGSIYVIRLLGRSFAFSSPGCTPFGFRLEIARER
                     AAGAVIGKSPS"
     gene            complement(522..1571)
                     /locus_tag="VGO02_08765"
     CDS             complement(522..1571)
                     /locus_tag="VGO02_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEV7477812.1"
                     /translation="MDTPALILDLDAFEKNLKTLQEAAGSRVRVRPHAKTHKCPEVAK
                     RQIALGAVGMCCQKVSEAEAMVEGGIADVLVSNEVVGAPKLARLAELSRRARLGVCVD
                     NAANLRDIGSSGARLDVYVELEVGMRRCGVAPGEPAVALAREIGKYPNLRFGGLQAYH
                     GRAQHIRGMDERHGVIETAAQHVFHTKKQLEKAGIACPVVTGAGSGTFMFEVGSGAWD
                     EIQPGSYAFMDADYARNEWAAPLPRFGHALFVQTTVMSRPSDQMAIVDAGLKASSVDS
                     GMPAVWQRSGLAYTHASDEHGWVEGPAVPGLGEKILLVPGHCDPTINLYDWYVCVRGG
                     VVEALWPITARGALL"
     gene            complement(1612..>1857)
                     /locus_tag="VGO02_08770"
     CDS             complement(1612..>1857)
                     /locus_tag="VGO02_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7477813.1"
                     /translation="GWNFLYIGGTTLLTETYRSEERAKAQGANDQAIFIMMALSSFTS
                     GLTVTSAGWATVNLYALPLVALIALAIGWYALRRRTA"
BASE COUNT          295 a          649 c          600 g          313 t
ORIGIN      
        1 cctcccggac gtcgccctgc gcaccttcgc cgccgatccc gcgaccgaga tgtcggaagc
       61 cttcgtcgaa gccacgcgcg cgaagggcga cgagtgctac gcgatccgcg acggcgcgaa
      121 ccttgccgcc tacggctggt acgcgaccac gccgacgacg gccggctcgc ccgagctgct
      181 gctgcatttc ggcgacgaat acgtgtacat gtacaaaggc ttcaccgacc cgcgctaccg
      241 cggccagcgg ctgcacgcga tcggcatgac gcgcgcgctg gaggaatacc gccgccgcgg
      301 ctaccgcgcg atggtgtcgt acgtcgagtc gaccaacttc gattccctga aatcctgctt
      361 caggatgggc tacagggtgt tcggctcgat ctatgtgatc cgcctcctcg gccgttcctt
      421 cgccttctcc agccctggct gcacgccgtt tggcttccgc ctcgagatcg cccgcgagcg
      481 cgccgccggc gcggtgatcg gcaagagccc ctcttagtcc gctacagcaa cgcccctctg
      541 gcggtgatcg gccagagcgc ttccaccacg ccgccgcgca cgcagacgta ccagtcgtag
      601 aggttgatgg tcgggtcgca gtggcctgga acgagcagga tcttttcacc gagccccggg
      661 acagcaggac cttccaccca accgtgctca tccgaggcat gggtataggc aagcccggac
      721 cgttgccata ccgccggcat gccggaatcg acgctcgaag ctttcaggcc tgcatccact
      781 atcgccatct ggtccgacgg gcggctcatc acggtggtct gcacgaacag cgcatgcccg
      841 aagcgcggca gcggcgccgc ccattcgttg cgcgcgtagt cggcgtccat gaaggcgtac
      901 gagccgggct ggatctcgtc ccaggcaccc gagccgacct cgaacatgaa cgtgccgctg
      961 cccgcgccgg tgacgacggg acaagcgatg cccgcctttt caagttgttt ctttgtatga
     1021 aaaacatgct gggcggccgt ttcgatcaca ccgtggcgct cgtccatgcc gcgaatgtgc
     1081 tgcgccctac cgtggtaagc ctgcaggccg ccaaagcgca ggttcgggta cttgccgatt
     1141 tctcgcgcca gcgcgactgc cggctcgccg ggtgcgacgc cgcagcggcg catgccgact
     1201 tcgagctcga cgtagacatc gagccgggcg cccgagctgc cgatgtcgcg cagattggcg
     1261 gcgttgtcga cgcacacgcc gagccgcgcg cggcgcgaca gctcggcgag gcgcgcgagc
     1321 ttcggcgcgc cgaccacctc gttgctgacc agcacgtcgg cgatgccgcc ttccaccatg
     1381 gcttcggcct cggagacctt ctggcagcac atgccgaccg cgccgagcgc gatctggcgc
     1441 ttcgccacct cggggcattt gtgcgtcttg gcgtgcggcc gcacgcgcac gcggctgcct
     1501 gccgcttcct gaagggtttt caaattcttc tcgaacgcat ccaggtcgag gatcagggcg
     1561 ggagtatcca cctgctccag cttctgcccg accgtggctg gggcgcgcat atcaggcggt
     1621 gcggcggcgc agcgcgtacc agccgatcgc cagcgcgatc agcgcgacca gcggcagcgc
     1681 gtagagattc accgtcgccc agccggcgct ggtcacggtg aggccggagg tgaaggaaga
     1741 cagcgccatc atgatgaaga tcgcctggtc gttggcgccc tgggccttgg cgcgctcctc
     1801 cgaccggtag gtctcggtga gcagggtcgt gccgccgatg tagaggaaat tccagcc
//
LOCUS       DASYJA010000399         2538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2510164, whole genome shotgun sequence.
ACCESSION   DASYJA010000399 DASYJA010000000
VERSION     DASYJA010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2510164"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..840)
                     /locus_tag="VGO02_08775"
     CDS             complement(46..840)
                     /locus_tag="VGO02_08775"
                     /inference="COORDINATES: protein motif:HMM:NF018439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HEV7477814.1"
                     /translation="MAPLAAAQSESRFEAFPVGTGFRSPLADPTEPRVYLGRLTVKRD
                     AGDVTAARAGIGYDLGVVRLQAGRPDDGWQLGVFGSIDSLFDLDLPGDALVNTDYRVG
                     LPLSWRNGAFSARARVYHQSSHLGDELILGGNAPSRINLSFQAVDALVAWQYGGWRVY
                     GGGSHVLTSSTDLYKGNGTQLGFDFVGSTSFYGMRPTAGVDVKWLEAADWHSGVSAKV
                     GLMFGRFSPDRRGFTIFLEAYDGFAPFGQFFAEDVKYYGAALQFDF"
     gene            complement(812..1246)
                     /locus_tag="VGO02_08780"
     CDS             complement(812..1246)
                     /locus_tag="VGO02_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7477815.1"
                     /translation="MAKLRHIAITVPDPWKAAEFYMRAFGLKKVGETDWANARGVYLS
                     DGEINIALLHYKTEEAAGHRGRDFVGVHHIGFLVDDVAASRRAIEAAGGTHWSGEPID
                     GGGFYEVKFHDPDGLVFDITKNGWAGASGDRAADGAARGGAE"
     gene            1276..2532
                     /locus_tag="VGO02_08785"
     CDS             1276..2532
                     /locus_tag="VGO02_08785"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006408212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV7477816.1"
                     /translation="MSLIVQKFGGTSVGSVDRIRNVARRVAKWQRAGHQLVVVPSAMA
                     GETNRLISLAKELQAQPDPRELDVIASTGEQVTIGLLSMALLELGVKARSYTGWQVRV
                     LTDSAFSKSRIISIDHEKLQYDLNDGYVVVVAGFQGVDAQGNVTTLGRGGSDTSAVAL
                     AAALKADECHIYTDVDGVYTTDPRLVPEARRLRTVTFEEMLEMAGAGSKVLQIRSVEF
                     AGKYKVPTRVLSSLTDPDLPVAEEGQAGTLVTFEEDPHMAMEKAVISGVAFNRDEAKI
                     TVKDVPDRPGIAYAILGPIADAHIDVDVIVQNVGHDGMTDMTFTVNRSDYAKALKILN
                     DKVKPHIKCREVSGDERIAKVSVVGMGMRSHAGIAAQMFRTLAEEGINIQMISTSEIK
                     ITVVVDEKYMELAVRVLHRAFELEQA"
BASE COUNT          467 a          819 c          851 g          401 t
ORIGIN      
        1 gtgcgatctc catgactctc ggagatcgca ccctgacccc gaattctaga agtcgaactg
       61 cagcgccgcg ccgtagtact tcacatcctc ggcgaagaac tggccgaacg gcgcgaagcc
      121 gtcgtaggcc tcgaggaaga tggtgaagcc gcgccggtcc ggcgagaagc ggccgaacat
      181 caggccgacc ttcgcgctga cgcccgagtg ccagtcggcg gcctccagcc atttcacgtc
      241 gacgcctgcg gtcgggcgca tgccgtaaaa cgaggtcgag ccgacgaaat cgaagccgag
      301 ctgcgtgccg ttacccttgt acaggtcggt gctcgaggtc agcacgtggc tgccgccgcc
      361 gtacacgcgc cagccgccgt attgccaggc gacaagggca tcgaccgcct ggaagctcag
      421 gttgatgcgg ctcggcgcgt tgccgccgag gatcagctcg tcgccgaggt gggagctctg
      481 gtggtacacg cgggcgcgcg cggaaaaagc gccgttgcgc caggacagcg gcagcccgac
      541 gcggtagtcg gtgttgacca gcgcgtcgcc gggcaggtcg aggtcgaaga gcgagtcgat
      601 cgagccgaac acgccgagct gccagccgtc gtcggggcgc ccggcctgca ggcgcacgac
      661 gccgaggtcg tagccgatgc cggcgcgcgc cgcggtgacg tcgccggcat cgcgcttcac
      721 ggtcaagcgc cccaggtaca cccgcggctc ggtgggatcg gcgagcggag agcgaaagcc
      781 ggtgccgacg ggaaaagcct cgaacctcga ttcactctgc gccgccgcga gcggcgccat
      841 cagcagccct atcgccagaa gcgccggccc agccgttttt cgtgatgtcg aagaccaggc
      901 catcgggatc gtggaatttc acctcataga agccgccgcc gtctatcggc tctccggacc
      961 agtgggtgcc tccggcggct tcgattgcgc gacgcgaagc agcgacgtcg tcgaccagga
     1021 atccgatgtg gtgcacgccg acgaaatcgc gcccgcgatg gcccgcggct tcctcggtct
     1081 tgtaatggag cagggcgatg tttatctcgc cgtcgctcaa gtacacgccg cgcgcgttgg
     1141 cccagtcggt ctcgccgacc ttcttcaggc cgaaggcgcg catgtagaac tcggctgctt
     1201 tccaagggtc cgggaccgtg attgcaatgt ggcggagttt ggccatgggt taaaatcctt
     1261 tgctttttca aagaaatgtc gctcatcgta caaaaattcg gcggcacctc ggtcggctct
     1321 gtggatcgca tccgcaacgt cgcgcgccgg gtcgccaagt ggcagcgggc cgggcaccag
     1381 ctggtggtcg tgccttcggc catggcgggc gagaccaacc gcctgatctc gctggcgaaa
     1441 gagctgcagg cgcagcccga tccgcgcgag ctcgacgtga tcgcctcgac cggcgagcag
     1501 gtgacgatcg gcctgctgtc gatggccctg ctcgagctgg gcgtgaaggc gcgcagctac
     1561 accggctggc aggtcagggt gctgaccgat tctgcttttt cgaagtccag aataatttca
     1621 atagaccacg aaaagctgca atacgatctg aatgacggct acgtggtggt cgtcgcgggc
     1681 ttccagggcg tggacgcgca gggcaacgtc accacgctcg gccgcggcgg ctccgacacc
     1741 tcggccgtgg cgctcgccgc ggctctcaag gccgacgagt gccatatcta taccgacgtc
     1801 gacggcgtct acaccaccga tccgcgcctg gtaccggaag cgcgcagact gcgcaccgtc
     1861 accttcgagg agatgctcga gatggcgggc gcaggctcca aggtgctgca gatccgctcg
     1921 gtggagttcg ccggcaagta caaggtgccg acgcgcgtgc tgtccagcct caccgacccc
     1981 gacctgccgg tggccgaaga ggggcaggcc ggcacgctcg tcactttcga ggaagacccg
     2041 cacatggcga tggaaaaggc agtgatatcc ggggtcgcgt tcaaccgcga cgaggcgaag
     2101 atcacggtga aggacgtgcc cgaccggccg ggcatcgcct acgccatcct cgggccgatc
     2161 gccgacgcgc acatcgacgt cgacgtcatc gtgcagaacg tcggccacga cggcatgacc
     2221 gacatgacct tcaccgtcaa ccgcagcgac tacgccaagg cgctgaagat cctcaacgac
     2281 aaggtgaagc cgcacatcaa gtgccgtgag gtgtcgggcg acgagcgcat cgccaaggta
     2341 tcggtggtcg gcatgggcat gcgctcgcac gccggcatcg cggcgcagat gttccgcacg
     2401 ctggcggagg agggcatcaa catccagatg atctccacct cggagatcaa gatcaccgtg
     2461 gtggtggacg agaagtacat ggagctcgcg gtgcgcgtgc tgcaccgcgc cttcgagctc
     2521 gagcaggcct gaccgtga
//
LOCUS       DASYJA010000400         4222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3056371, whole genome shotgun sequence.
ACCESSION   DASYJA010000400 DASYJA010000000
VERSION     DASYJA010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3056371"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /gene="pncB"
                     /locus_tag="VGO02_08790"
     CDS             complement(<1..816)
                     /gene="pncB"
                     /locus_tag="VGO02_08790"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEV7477817.1"
                     /translation="MIIQSLLDTDLYKFTMMQVVLHHFPGAQVEYKFRCRTEGVDLQP
                     DLPEIRAELEKLCSLRFHDEELAYLRSLRFMKSDFVDFLALFHFSERYVRLGPGEKPG
                     EIDITVGGPWLHTIMFEIPLLAIVSELYFRRTQPRPDLAEGRRRLKAKIDVARAVQPE
                     LGFRISDYGTRRRFSRDWQEEVLQTFKREIPQNFAGTSNVWFAMRNNVTPLGTMAHEY
                     MQACQAVGPRLRDSQVFAFNTWAREYRGDLGIALSDVCGMDAFLRDFDLFFCKL"
     gene            856..1410
                     /locus_tag="VGO02_08795"
     CDS             856..1410
                     /locus_tag="VGO02_08795"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEV7477818.1"
                     /translation="MCGRYALHSNPEVVALQFHLDAVPAFLPRYNIAPAAQILAIKPE
                     GAVLLKWGWRGKAHNLRAETTRRGRRCLIPANGFYEWRKVGAGKQPYYVRPARGALFG
                     FAGIWDEATCAILTVEANRTLRAIHHRMPAIVAPQSYARWLGGDDDLLAPAPEEETVA
                     YPVGPAINSGSAESPRLIEPSGID"
     gene            complement(1396..2427)
                     /gene="dinB"
                     /locus_tag="VGO02_08800"
     CDS             complement(1396..2427)
                     /gene="dinB"
                     /locus_tag="VGO02_08800"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEV7477819.1"
                     /translation="MRRIVHLDMDAFYASVEQRDAPALRGRPVAVGGRPEGRGVVAAS
                     SYEARQFGVRSAMPMARALRLCPQLEIVRPDFEKYRAVSGQVMAILRDCTPLVEPLSL
                     DEAYLDVTTNSFNEEFGSSVARRLKQQIKDQTGLSASAGVAPNKFLAKIASGWRKPDG
                     LTVIAPERVEAFLQQLPVEALWGVGPVTARKLREAGIERLVQVRAFDPAELAKLVGGM
                     ADWLRRLSYGDDPRPVQPDRPAKSSSSENTYAEDLVDLGAIRAEIDRMARENAEWLAR
                     KRLAAKSIDIKVRYADFSTVTRSHTLSVPTSDGDVIASWAMMLLEKTEAGRRPVRLLG
                     AGVHGLVDA"
     gene            2447..3073
                     /gene="lexA"
                     /locus_tag="VGO02_08805"
     CDS             2447..3073
                     /gene="lexA"
                     /locus_tag="VGO02_08805"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEV7477820.1"
                     /translation="MDELTDRQGEILRLIRELTEVSGFPPTRAEIAERMGFRSVNAAE
                     QHLRALEKKGAIEITAGSSRGIKVCDSRSASRGRLMELPVIGRVAAGSPMLAEEHVQE
                     RYQVDPNLFTPRADYLLRVRGMSMRDAGILEGDLLAVHRTEEARTGQIVVARLADEVT
                     VKRLRRRGHAVQLVAENPDFEPIEVDLRRDPLVIEGIAVGVIRNGKSL"
     gene            3113..>4222
                     /locus_tag="VGO02_08810"
     CDS             3113..>4222
                     /locus_tag="VGO02_08810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV7477821.1"
                     /translation="MDPTPTATVQGAEPLPASSAGSVSASIRDRADPRLVLADRVEQL
                     YSQMWLGILMTFAIGAIATFELWENRLRELVLFWWGIVLLITASSAGLVIAYRRAPDR
                     ATQPEQWLRWLGIAALANGAMWGFAGAVFFPSHADEQQVFLAFLLAGMMSAGIPVYAA
                     SWPIFAVYAAGIVAPFTYVLATFGNRLFTELAVLVPMFYAINVAIAYRLSDVFHSGYR
                     LRRAYGRLTDDYSVLNQQLERQLVELEEARRQVEASGRKLALFAERAPIAVLELDPHG
                     TVTQINHAAELLFGYAAAELVGASVKRIVRPEFHAEFDSQWAALIASREPVAAIKIRN
                     PRKDGQDLVCEWAVTPLVNPEGTILSIIAQGQDVTQ"
BASE COUNT          677 a         1465 c         1409 g          671 t
ORIGIN      
        1 cagcttgcag aagaaaagat cgaaatcgcg caggaaggcg tccatgccgc agacgtcgga
       61 cagcgcgatt cccagatcgc cgcggtattc gcgcgcccag gtgttgaacg cgaacacctg
      121 cgaatcgcgc agccgcggtc ccaccgcctg gcaggcctgc atgtactcgt gcgccatggt
      181 gccgaggggc gtcacgttgt tgcgcatcgc gaaccagacg ttggaggtgc cggcgaagtt
      241 ctgcgggatc tcgcgcttga aggtctgcag cacctcctcc tgccagtcgc gcgagaagcg
      301 ccgccgcgtg ccgtagtcgg agatcctgaa gcccagctcg ggctgcaccg cgcgcgccac
      361 gtcgatcttg gccttcagcc gcctgcggcc ttcggcaaga tcgggcctgg gctgcgtgcg
      421 cctgaagtag agctcggaga cgatggcgag cagcgggatc tcgaacatga tggtgtgcag
      481 ccacggcccg ccgaccgtga tgtcgatctc gccgggcttc tcgccgggcc cgaggcgcac
      541 gtagcgctcg ctgaaatgga acagggcgag gaaatcgacg aagtccgact tcatgaaccg
      601 caggctgcgc aggtaggcga gctcctcgtc gtggaagcgc aggctgcaca gcttttcgag
      661 ctcggcgcgg atctcgggca ggtcgggctg cagatcgacg ccctcggtgc ggcagcggaa
      721 cttgtattcg acctgcgccc ccgggaaatg atgcagcacc acctgcatca tcgtgaactt
      781 gtagaggtcg gtgtcgagca gcgactggat gatcatgccg cggccattgt aaccggggcg
      841 gttatcctat atgccatgtg cggccgttac gcgctccaca gcaacccgga agtcgtcgcg
      901 ctgcagtttc acctggacgc ggttccggcg ttcctgccgc gctacaacat cgcgcccgcc
      961 gcccaaatac tggcgataaa gccggagggc gccgtcctgc tgaaatgggg atggcgcggc
     1021 aaggcgcaca acctgcgcgc cgaaacaacc cgcaggggcc gccgctgcct gatcccggcg
     1081 aacggcttct acgagtggcg caaggtcggg gcgggcaagc agccgtacta cgtgcgcccc
     1141 gcacggggcg cccttttcgg tttcgccgga atctgggacg aggcaacctg cgccatcctc
     1201 acggtcgaag cgaaccgtac gctgcgcgcc atccaccatc gcatgccggc gatcgtcgcg
     1261 ccgcaaagct atgcgcgctg gcttggcggc gacgatgacc tgctcgcgcc ggcgccggaa
     1321 gaagagaccg tcgcctatcc cgtcggcccc gcgatcaaca gcggcagcgc ggagtcgccg
     1381 cggctgatcg agccctcagg catcgactag cccgtgcacg ccggcgccga gcaggcgcac
     1441 cgggcggcgg ccggcttcgg tcttctcgag cagcatcatc gcccaggagg cgatcacgtc
     1501 gccgtcgctg gtcgggaccg aaagcgtgtg gctgcgcgtg acggtggaga agtcggcgta
     1561 gcgcaccttg atgtcgatgc tcttcgcggc gagccgcttg cgcgccagcc actcggcgtt
     1621 ttcgcgtgcc atgcggtcga tctcggcgcg gatcgcgccc aggtcgacca ggtcctcggc
     1681 gtacgtgttc tcgctgctgg aggatttcgc cgggcggtcg ggctgcaccg ggcgcgggtc
     1741 gtcgccgtac gaaaggcgcc gcagccagtc ggccatgccg ccgaccagct tcgccagctc
     1801 ggcggggtcg aaggccctca cctgcaccag ccgctcgatg ccggcctcgc gcagcttgcg
     1861 ggcagtgacc ggcccgacgc cccatagcgc ctcgaccggc agctgctgca aaaacgcctc
     1921 gacccgctcg ggcgcgatca ccgtcaggcc gtcgggcttg cgccagcccg aggcgatctt
     1981 ggcgagaaac ttgttcggcg ccacgccggc cgaagcgctc aggccggtct gatccttgat
     2041 ctgctgtttc aacctgcgtg caacgctgct gccgaattct tcattgaaag aattggtcgt
     2101 gacgtcgagg taggcctcgt ccagcgagag cggctcgacc agcggcgtgc aatcgcgcag
     2161 gatcgccatc acctggcccg acacggcgcg gtacttctcg aagtcgggcc gcacgatctc
     2221 gagctgcggg cacaggcgca gggcgcgcgc catcggcatc gccgagcgca cgccgaactg
     2281 gcgcgcctcg tagctcgatg ccgcgaccac gccgcgcccc tcgggccggc cgccgaccgc
     2341 caccggcctg ccgcgcagcg ccggtgcgtc gcgctgctcg accgacgcgt agaaggcgtc
     2401 catgtccaaa tggacaatcc gccgcatact gtatatattg ccacatatgg atgaattaac
     2461 agaccgccag ggagaaatcc tccgactgat ccgggagttg accgaggttt cgggcttccc
     2521 acccacccgc gccgagattg cggagcgaat ggggttccgc tcggtgaatg ccgccgagca
     2581 gcacctgcgg gcgctggaga agaagggggc gatcgaaatt accgcgggct cctcgcgcgg
     2641 catcaaggta tgcgacagcc gttccgccag ccgcgggcgc ctgatggagc tgcccgtgat
     2701 cggccgcgtg gccgccggca gcccgatgct cgccgaagag cacgtgcagg agcgctacca
     2761 ggtcgacccc aaccttttca ccccgcgcgc cgactatctg ctgcgcgtgc gcggcatgtc
     2821 gatgcgcgac gccggtatcc tcgagggcga cctgctcgcc gtgcatcgca ccgaggaggc
     2881 gcgcaccggg cagatcgtgg tcgcccggct ggccgacgaa gtcaccgtaa aaaggctgcg
     2941 gcgccgcggc cacgcggtgc agctcgtagc ggagaatccg gacttcgagc cgatcgaagt
     3001 cgacctgcgg cgcgatccgc tggtcatcga gggaatcgcc gtaggcgtaa tccgcaacgg
     3061 caaaagcctg taagttgtcg ctagaattgc gcaggctccc tgcctgcgct tgatggaccc
     3121 aacccctacc gcgaccgttc aaggcgccga acccctgccc gcatcctccg cggggagcgt
     3181 ctcggccagc atccgcgacc gcgccgatcc gcggctggtg ctcgccgacc gcgtcgagca
     3241 gctgtacagc cagatgtggc tcggcatcct gatgaccttc gccatcggcg ccatcgccac
     3301 cttcgagctg tgggagaacc gcctcaggga gctggtgctg ttctggtggg gcatcgtgct
     3361 gctcatcacc gcctcgagcg ccggcctggt gatcgcatac cggcgcgccc ccgaccgcgc
     3421 cacccagccc gagcagtggc tgcgctggct cggcatcgcc gcgctcgcca acggcgcgat
     3481 gtggggcttc gccggcgcgg tcttcttccc gtcgcacgcc gacgagcagc aggtgttcct
     3541 cgccttcctg ctcgccggca tgatgtcggc cggcatcccg gtgtacgccg cctcctggcc
     3601 gatcttcgcc gtgtatgccg ccggcatcgt ggcgccgttc acctatgtcc tcgccacctt
     3661 cggcaaccgc ctgtttaccg agctggccgt gctggtgccg atgttctacg ccatcaacgt
     3721 ggcgatcgcc taccggctga gcgacgtgtt ccactcgggc taccggctgc gccgcgccta
     3781 cggcaggctg accgacgact acagcgtgct caaccagcag ctcgagcgcc agctggtcga
     3841 gctggaagag gcgcgccgcc aggtcgaggc gagcggccgc aagctcgccc tgttcgccga
     3901 gcgcgcgccg atcgcggtgc tcgagctcga cccgcatggc accgtcaccc agatcaacca
     3961 tgccgcggag ctcctcttcg gctatgccgc cgccgagctg gtcggcgctt ctgtgaagcg
     4021 catcgtgcgg ccggaattcc acgccgagtt cgacagccag tgggcggcgc tgatcgcttc
     4081 gcgcgagccg gtggcggcga tcaagatccg caacccgcgc aaggatggcc aggacctggt
     4141 gtgcgagtgg gcggtgacgc cgctggtgaa cccggagggc accatcctct cgatcatcgc
     4201 gcaggggcag gacgtcaccc ag
//
LOCUS       DASYJA010000401         4523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2965254, whole genome shotgun sequence.
ACCESSION   DASYJA010000401 DASYJA010000000
VERSION     DASYJA010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2965254"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1516
                     /locus_tag="VGO02_08815"
     CDS             <1..1516
                     /locus_tag="VGO02_08815"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEV7477822.1"
                     /translation="WSILATPKLNGRLAVRRAAGDLALAGPPEVPLELSRATLDARFT
                     DDRVAAAIEVATRLGSARIEGEAAGFTPQSAASYTAEIDLKELRLLTEPLFTQVRISG
                     RVGATLKGAGTLGKPLLTGTLRGDALGVEMPPYGVALTGGRLRAELEGDRLRIVEAAI
                     AGGEGRFSLRGSMPLRLAEGGTALDWQAEKFRVLNRPDTRLVVSGKGSATFDGKKLGL
                     AGELRADSGHFEVRGSGLPVLDDDVVVAGRGPSAREKRAPLPIDLDLRLDLGERLTLR
                     AFGFDGGVAGQLRVVTDTSGQLLAQGRLRAVRARFAAYGQQLEVDPGMLIFAGPLDRP
                     ALDITAWRRHQAVEAGVHITGSVELPRVELVSNPPVNDADKLTWLVLGRAPSTASGAD
                     LAILQAASGALVGMSGDQVPVQRRIASRLGLDEITVRSSTELATRNSTDPTTNVFALG
                     KRLSDKLYVSFEQAIGTTAEYLVKLDYSLTQRVSVRGQTGTTSGVGLFYRYAWD"
     gene            1536..1943
                     /locus_tag="VGO02_08820"
     CDS             1536..1943
                     /locus_tag="VGO02_08820"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV7477823.1"
                     /translation="MARLAALFFLLLAGCGAADPGAGLATIESAAVGLALRELPRPIL
                     RSLGLPYGLAVVKTEAAAAHAGLRPGDVIYGVNQSRIGSIDDFARLVAEQPPGVPLAL
                     LVRRGGTDFYLALDPGGIARSPRRRPTDTLLRT"
     gene            complement(1918..2172)
                     /locus_tag="VGO02_08825"
     CDS             complement(1918..2172)
                     /locus_tag="VGO02_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477824.1"
                     /translation="MKHIIFSAAMVLSASAFGQAAKPEPIKAEPIKAEPAKPQAAASV
                     AAKPKAPKPAKHTNRRQEDARQCLERPTNTEIIKCAEEYL"
     gene            complement(2220..3467)
                     /locus_tag="VGO02_08830"
     CDS             complement(2220..3467)
                     /locus_tag="VGO02_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEV7477825.1"
                     /translation="MDGDMGDGSLASFTAPVHGRLELASAFQPIFSPAHRRAVGYEGL
                     IRARDKRGREVPAGETRILLDRQCRSLHVANFMDFGDERSWLFLNIDPYVAIEGRNYG
                     SFFAGMLRAAGLSPHRVAVELIESPLADEGRLAAAMEYYRELGCIIVLDDFGAGHSNF
                     DRIWRLNPDIVKIDREMTRRVTVEPLARRMFSGIVSVLHEAGALVCVEGIETEDEALC
                     ATDANADLVQGHYFASPARELPAEEICRDTFTHLFSGFRAEMLEAQARREARLEPYLE
                     ALKTAGRALAAGDGHGAAVVPLLALSCTQRCYLIAGDGSQRGDNLESEHNVSARDPRL
                     EPMRPTPGTNWQNKPYFRRAVAAPGAVQVTRPYLSVTGPKLCVTLSLACEVEGALYVL
                     CADLDFTALAGEDLSFHSARPAA"
     gene            3628..4137
                     /locus_tag="VGO02_08835"
     CDS             3628..4137
                     /locus_tag="VGO02_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEV7477826.1"
                     /translation="MTIKVGDKLPDGKLMESTEFDSATQCAMPPKEVNVGEAVKGKKI
                     AVFGLPGAYTPTCSAKHVPGYVQNLDQLKKKGVSEIWCVATNDAFVMAHWGKDQKALG
                     KIRFLADGSAQWAKALGLELDLNARGMGTRMNRFSMLVDNGVVKQLNVEGPGKFEVSD
                     AATMLKQLG"
     gene            4220..>4523
                     /locus_tag="VGO02_08840"
     CDS             4220..>4523
                     /locus_tag="VGO02_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477827.1"
                     /translation="MFKTIFAALLLAASTSLFAQAPQGDAAKAPHKARFDCSQAKDPK
                     ACEERRAKMKAAHEKAAKACESKKGADHVACMRTEMCAQSKDPKGCEAKAKEATARR"
BASE COUNT          720 a         1529 c         1606 g          668 t
ORIGIN      
        1 ctggtcgatc ctggcgacgc cgaagctgaa cggccgcctc gcggtgcgcc gcgccgcggg
       61 cgacctggcg ctcgccgggc cgcccgaggt gccgctcgaa ctgtcgcgcg ccacgctcga
      121 tgcgcgcttc accgacgatc gcgtcgccgc cgcgatcgag gtggccacgc gcctgggcag
      181 cgcgcgcatc gaaggcgagg ccgcggggtt cacgccgcaa tcggcggcca gctacaccgc
      241 cgagatcgat ctgaaagagc tgcgtctgct gaccgagccg ctcttcacgc aggtgcgcat
      301 ctccggacgc gtcggcgcga cgctcaaggg cgccggcacg ctgggcaagc ccctcctgac
      361 gggaacattg cgcggcgacg cgctcggcgt cgaaatgccg ccctacggcg tcgccctcac
      421 cggcggccgc ctgcgcgccg agctggaggg cgaccggctg cgcatcgtcg aggcggcgat
      481 cgccggcggc gaaggccgct tcagcctgcg cggctcgatg ccgctgcggc tcgccgaagg
      541 cggcaccgcg ctcgactggc aggcggagaa gttccgcgtg ctgaaccgcc ccgacacgcg
      601 gctcgtcgtc tccggcaagg gcagcgcgac gttcgacggc aagaagctcg gcctggccgg
      661 cgagctgcgc gccgacagcg gccatttcga ggtacgcggc agcggcctgc cggtgctcga
      721 cgacgacgtg gtcgtcgccg ggcgcggccc cagcgcccgc gagaagcgcg cgccgctgcc
      781 gatcgacctc gacctgcgcc tggacctcgg cgagcggctg acgctgcgcg ccttcggctt
      841 cgacggcggc gtcgccgggc agctgcgcgt agtgaccgac acctcgggcc agctgctcgc
      901 gcaaggcagg ctgcgcgccg tgcgcgcgcg cttcgccgcg tacgggcagc agctcgaggt
      961 cgaccccggc atgctgatct tcgccgggcc gctcgaccgc ccggcgctcg acatcacggc
     1021 ctggcgccgc caccaggcgg tggaggccgg ggtgcacatc accggcagcg tggagctgcc
     1081 ccgggtcgag ctggtctcca acccgccggt gaacgatgcc gacaagctga cctggctggt
     1141 gctcggccgc gcgcccagca cggcgagcgg cgcggacctc gccatcctgc aggcggcgag
     1201 cggcgcgctg gtcggcatga gcggcgacca ggtgccggtg cagcggcgca tcgcctcgcg
     1261 cctcggcctg gacgagatca ccgtgcgcag ctcgaccgag ctcgcaacgc gcaactcgac
     1321 cgacccgacg accaacgtct tcgcgctcgg caagcgcctt tcggacaagc tctacgtgag
     1381 cttcgagcag gcgatcggca cgaccgcgga atacctggtc aagctcgact actcgctcac
     1441 gcagcgtgtt tcggtgcgcg gccagaccgg cacgaccagc ggcgtgggcc tgttctaccg
     1501 ctacgcctgg gactgaaatt tgcatcgggc cggccatggc ccggcttgcc gctttgtttt
     1561 tccttcttct ggcaggctgc ggcgcagcgg acccgggcgc cggcctggcg acgatcgaaa
     1621 gcgcggcggt cggcctggcg ttgcgcgagc tgccgcgccc gatcctgcgc tcgctggggt
     1681 tgccctacgg gctggcagtg gtcaagacgg aggccgcggc ggcgcacgcc ggactgcgcc
     1741 cgggggacgt gatctacggg gtgaaccaga gccgcatcgg cagcatcgac gacttcgccc
     1801 ggctggtagc ggagcagccg cccggggtgc cgctcgcgct gctcgtgcgg cgcggcggga
     1861 ccgacttcta tctggcgctg gaccctggcg gaatcgcgcg ctcgccgcgc cggcggccta
     1921 cagatactct tctgcgcact tgatgatttc ggtgttggtc ggccgctcga ggcattgacg
     1981 cgcgtcctct tggcgccgat tggtgtgctt cgccggcttg ggcgccttgg gcttggcggc
     2041 aacgctcgcc gcggcctgcg gcttggcggg ctcggctttg ataggttcgg ccttgatcgg
     2101 ctcaggcttg gcggcctggc cgaaggcgct ggccgacaga accatggctg cagaaaagat
     2161 aatgtgtttc atggacctac cccttgttcc ttaagggata ggcgaagcat atcctatatt
     2221 caggcggcgg gccttgcgct gtggaaggac aggtcctcgc cggcaagggc ggtgaagtcg
     2281 aggtcggcgc acagcacgta cagcgcgcct tcgacctcgc aggcaagcga cagggtgacg
     2341 cacagcttcg ggccggtgac cgagaggtaa ggccgggtca cctgcaccgc gccgggcgcg
     2401 gcaacggctc ggcgaaagta gggcttgttc tgccagttgg tgcctggggt cgggcgcatc
     2461 ggctcgaggc gcgggtcgcg cgccgagacg ttgtgctcgg actcgaggtt gtcgccgcgc
     2521 tgcgagccgt cgccggcgat caggtagcag cgctgggtgc aggacagcgc gagcagcggc
     2581 accactgctg ccccgtgccc gtcgcccgcg gcgagcgcgc gcccggcagt cttcagggcc
     2641 tccaggtagg gctcgaggcg cgcttcgcgg cgcgcctggg cctcgagcat ctcggcgcga
     2701 aagccgctga acaggtgcgt gaaggtgtcg cggcagatct cctcggccgg cagctcgcgc
     2761 gccgggctgg cgaagtaatg cccctgcacc aggtcggcgt tggcgtcggt cgcgcacagc
     2821 gcctcgtcct cggtctcgat gccctcgacg cacaccagcg cgcccgcctc gtgcagcacc
     2881 gagacgatgc cgctgaacat gcgccgcgcc agcggctcga ccgtcacgcg gcgcgtcatc
     2941 tcgcggtcga tcttcacgat gtcgggattg agccgccaga tgcggtcgaa gttggaatgg
     3001 ccggcgccga aatcgtcgag cacgatgatg cagcccagct cgcggtagta ctccatcgcc
     3061 gcggcgaggc ggccctcgtc ggcgagcggc gactcgatca gctcgaccgc cacgcggtgc
     3121 ggcgacaggc cagccgcgcg cagcatgccg gcgaagaacg agccgtagtt gcggccctcg
     3181 atggcgacgt agggatcgat gttgaggaac agccagctgc gctcgtcgcc gaagtccatg
     3241 aagttggcga cgtgcaggct gcggcactgc cggtcgagca ggatgcgcgt ttccccggcc
     3301 ggcacctcgc ggccgcgttt gtcgcgcgcg cggatcagcc cctcgtagcc gaccgccctg
     3361 cggtgcgcgg ggctgaagat cggctggaaa gcgctggcaa gctcaaggcg cccatgcacc
     3421 ggcgccgtga acgaagcgag cgacccatcc cccatgtctc cgtccatgcg ttgtcttgcc
     3481 gtcccctgag cggcagaagt gtgccagcaa tttagtgcct gttttcaggg ctgaatgtga
     3541 aatattgggt ggagggcggt tgcgccgcac aaaagggcat ttgggtatca tctgccctcc
     3601 cccgaacacc ccaacaggag attccccatg acgatcaagg tcggcgacaa gctgcctgac
     3661 ggcaaactga tggaaagcac cgaattcgac tccgccacgc agtgcgcgat gccgcccaag
     3721 gaggtcaacg tcggcgaggc ggtcaagggc aagaagatcg cggtgttcgg cctgccgggc
     3781 gcctacacgc cgacctgctc ggcgaagcac gtgccgggct acgtgcagaa cctcgaccag
     3841 ctgaagaaga agggcgtgag cgagatctgg tgcgtcgcga ccaacgacgc cttcgtcatg
     3901 gcgcattggg gcaaggacca gaaagcgctc ggcaagatcc gcttcctcgc cgatggcagc
     3961 gcgcaatggg cgaaggcgct cggcctggag ctcgacctca acgcccgcgg catgggcacg
     4021 cgcatgaacc gcttctcgat gctggtcgac aacggcgtgg tcaagcagct caacgtggaa
     4081 ggccccggca agttcgaggt gtcggatgcc gcgaccatgc tgaagcagct cggatagata
     4141 caaagggtta caaagaagct gtcagcggcc gggctggaag caacgtttta ggaaggtctc
     4201 ttccagaaaa ggatctttca tgttcaaaac gatatttgct gcgctgctcc tggcagcttc
     4261 caccagcctg ttcgcccagg cgccgcaggg cgacgcggcc aaagctccac ataaggcgcg
     4321 cttcgactgc tcgcaggcga aggatcccaa agcctgcgag gagcgccgcg ccaagatgaa
     4381 ggccgcgcac gagaaggccg ccaaggcctg cgaaagcaag aaaggcgcgg accacgtggc
     4441 ctgcatgcgc accgagatgt gcgcccagtc gaaggacccg aagggctgcg aggcgaaggc
     4501 gaaggaagcc acggcccggc gcg
//
LOCUS       DASYJA010000402         6013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_240983, whole genome shotgun sequence.
ACCESSION   DASYJA010000402 DASYJA010000000
VERSION     DASYJA010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240983"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VGO02_08845"
     CDS             complement(<1..344)
                     /locus_tag="VGO02_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEV7477828.1"
                     /translation="MLSFEEEGKVGAIPGLVVAPTSAFVPGGAAKEPPARVDSRLRRV
                     SVEDKLVINSKADVNQLVPFKYKWAWEKYIAACANHWMPQEINMSRDIQLWKDPNGLT
                     EDERRVVKRNLG"
     gene            complement(331..675)
                     /locus_tag="VGO02_08850"
     CDS             complement(331..675)
                     /locus_tag="VGO02_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477829.1"
                     /translation="MISLGGRSFAGPFLAPVWTAPRAAGLYAVMVPGWRLLTFHPVHI
                     GHASDLAYAGLLRGHDHYAEWLSLAGTEWNLYVAACEMAGSSELERQAACRELARTQQ
                     TVTLKGPSHAQL"
     gene            complement(672..3470)
                     /locus_tag="VGO02_08855"
                     /pseudo
     CDS             complement(672..3470)
                     /locus_tag="VGO02_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
     gene            complement(3889..4431)
                     /gene="ampD"
                     /locus_tag="VGO02_08860"
     CDS             complement(3889..4431)
                     /gene="ampD"
                     /locus_tag="VGO02_08860"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEV7477830.1"
                     /translation="MKLGPDGWLEDARRVPSPNRDERPEGSEISLLLLHAISLPPGEY
                     GGDAIERLFTNTLDPDAHPYFREVFCLQVSSHFLVRRDGALLQFVPVHERAWHAGASA
                     WRGRSRCNDFSIGIELEGSEEEAFAGVQYSTLAALARCLRASLSLRDIAAHSEVAPGR
                     KTDPGPHFDWARLLADLAQA"
     gene            complement(4428..5807)
                     /locus_tag="VGO02_08865"
     CDS             complement(4428..5807)
                     /locus_tag="VGO02_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEV7477831.1"
                     /translation="MKRAERRSGPRGAQVLVVDDEPDIRELLELTLVKMGLGVASVGS
                     IAEAKELLKGEERYALCLTDMRLADGEGLDLVRHIAGLGADLPVAVITAYGSAENAVA
                     ALKAGAFDYVSKPVGLEQLRALVKSALSLPDRVMAPAKGQQLLGASQPLVLVREMIGK
                     LARTQAPVYISGESGSGKELAARLIHDNGARREQPFVPVNCGAIPETLMESEFFGYKK
                     GAFTGAGEDRDGFFQAANGGTLFLDEVAELPLPMQVKLLRTIQEKRVRKVGATQEDPV
                     DVRIICATHQNLGALVAAGRFRQDLFYRLNVIELVMPPLRDCREDIALIAAAILARLG
                     GQAGGGPARLSPRALEELARYDFPGNIRELENVLERAVALSGAEEIGVEDLRLEPQRL
                     EADRDSAGPSVESLPDYLDGLERKAILEALAKTGFNRTAAAKLLGITFRQLRYRMQRL
                     GIREEKPAA"
     gene            complement(5804..>6013)
                     /locus_tag="VGO02_08870"
     CDS             complement(5804..>6013)
                     /locus_tag="VGO02_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV7477832.1"
                     /translation="LSVIDNGPGVPAADQGQLFEPFFTTESKGTGLGLYLARELCSAN
                     RAILEYVDDLPGAHFRILCREARAA"
BASE COUNT          933 a         2015 c         2017 g         1048 t
ORIGIN      
        1 aagccgaggt tgcgcttcac cacccggcgc tcgtcctcgg tcaggccgtt cgggtccttc
       61 cacagctgga tgtcgcggct catgttgatt tcctgcggca tccagtggtt ggcgcaggcg
      121 gcgatgtact tctcccaggc ccacttgtac ttgaacggca ccagctggtt gacgtcggcc
      181 ttggaattga tgaccagctt gtcctcgacg ctgacgcgcc gcaggcggct gtcgacgcgc
      241 gccggcggct ccttggcggc gccgcccggc acgaaggcgc tggtcggcgc caccaccagg
      301 ccggggatcg cgcccacctt gccctcttcc tcaaagctga gcatggctcg gccccttcaa
      361 ggtgacggtc tgctgcgtcc tggcgagctc gcggcacgcc gcctgccgct cgagctcgct
      421 cgagccggcc atctcgcagg cggcgacgta caggttccac tcggtgccgg cgagggacag
      481 ccactcggcg tagtggtcgt gtccgcgcag cagcccggcg taggccaggt cggaggcatg
      541 gccgatgtgc accgggtgga aggtgagcag gcgccagccc ggcaccatca ctgcgtacag
      601 cccggcggcg cgcggcgccg tccacaccgg cgcgaggaac ggcccggcga agctgcgccc
      661 gccgagggag atcattggca ggcctcgcac tcgggattgt cgatcgagca gaacttcggg
      721 tccgccgccg cggcctcggc ggtatccgcg gctccggaca gctgggcgcc ggagctcgac
      781 accgcgttga gcgcgccggc cttgctggtc gacttctcgg cgtgcgtcgc gcccatcgag
      841 cgcaggtagt aggtggtctt caggccgcgg atccaggcga gcttgtaggt ctcgtcgagc
      901 cgcttgcccg aggcgccggc catgtagatg ttgagcgact gcgactggtc gatccatttc
      961 tggcggcgcg cgcccgcctc gaccagccac ttcggctcga cctcgaacgc ggtcgcatac
     1021 agccggcgca gctccgccgg cgcgcggtcg atgcgcgcca ggttgccgtc gaagtacttg
     1081 aggtcggcga tcatcacctc gtcccacagc ccggcctttt tcaggtcggc caccaggtac
     1141 tcgttggtca ccgtgaactc gcccgacagg ttcgatttca cgaacaggtt ctggtaggtc
     1201 ggctcgatcg aggcggagac gccgacgatg ttggcgatgg tcgcggtcgg cgcgatggcg
     1261 atgcagttgg agttgcgcat gccgtggcgc ttgatgcggc cgcgcagcgc ttcccagtcg
     1321 aggctcgccg agcggtcgac ctcgacgtag ccgccgcgct cggcggcgag cagatcgagc
     1381 gtgtcctgcg gcagcaggcc gcggtcccac agcgagccgg ggaagctcga gtagcggccg
     1441 cgctcctcgg cgagctcggt cgaggcccag taggcggcga agcacaccgt ctccatcgag
     1501 cggtcggcga agtcgaccgc ttcctgcgag gcgtagggga tgcgcagacg gtgcaggcag
     1561 tcctggaagc ccatgatgcc caggccgacc gggcggtggc gcaggttgga attgcgcgcc
     1621 ttgtcgaccg cgtagtagtt gatgtcgatg acgttgtcga gcatccgcat ggccgtgcgg
     1681 atagtgttct tcaatttgga gaagtccaac ccctggtcat tcaggtgcgc aaccaggttt
     1741 acggagccga ggttgcacac cgcgatctca tcggccgagg tgttgagggt gatctcggtg
     1801 cacaggttgg agctgtgcac cacgccgacg tgctgctgcg ggctcctcag gttgcacggg
     1861 tccttgaagg tgatccacgg atgcccggtc tcgaacagca tcgacagcat cttgcgccac
     1921 agctgcactg ccgggagctt cttgtgcagc ttgagatcgc cgcgcgccac cttgtcctcg
     1981 tagcgcaggt aggcctcctc gaagtccttg ccgaacttgt cgtgcagctc gcgcacatcc
     2041 gaggggctga acaacgtcca gtctgcattt tccattaccc gcttcatgaa caggtccggg
     2101 atccagttgg ccgtgttcat gtcgtgggtg cggcgccggt cgtcgccggt gttcttgcgc
     2161 agctcgagga actcctcgat gtcgagatgc caggtctcca gatatgagca gacggcaccc
     2221 ttgcgtttgc cgccctgatt caccgccacc gcggtgtcgt tcaccacttt caggaacggc
     2281 accacgccct gcgacttgcc gttggtgccc ttgatgtgcg agccgagggc gcgcaccggg
     2341 gtccagtcgt tgcccaggcc gccggcatac ttggcgagca gggcgttctc cttgatcgcg
     2401 tcgtagatgc cctcgaggtt gtcggacacc gtggtcaggt agcagctgga aagctgcgag
     2461 cgctgcgtgc cggcgttgaa cagggtcggc gtcgagctca tgaagtcgaa gctcgacagc
     2521 acgtcgtaga acgcaattgc tttttcttcc ctattttttt ctttaagagc caaccccatg
     2581 gctacgcgca tgaagaacgc ctgcggcagc tcgatgcgct ggtcgcgcac gtgcaggaag
     2641 tagcggtcat acagcgtctg cagcccgagg tagccgaact gcagatcgcg gcgggccttg
     2701 agcgcttttc ccaatacatc caaattaaaa cgaccaagct ccggatcgag cagctcggcg
     2761 gcgatgccgc gcgcgatgaa ctgcgggaag taggcggcgt agcgggtctg catctccgcc
     2821 tggctcgcct ccccgcccag cacctcgagg cggatggtgt tgaggagcag gcgcgcggtc
     2881 acatagctgt aggccgggtc tttttcgatc agcgcgcggg cggcgagcac cgccgacttg
     2941 cgcacctcgt ccatcggcac gccgtcgtac aggtcgcgca gcgtcgaatc gaggatcgcc
     3001 tgcgggctcg cgtcgggcag gccctcgcag gccgccgtca ccagctgcgc cagcgcctcg
     3061 cggtcgagcg gctttctctg cccctgctcg acgacatgga tgccgccctc gccgccgttc
     3121 gccaagcggc gcgccgcctt ctcgttcgag cgcgccttgg agcgctcctc ccggtagagc
     3181 acataggagc gcgccacgtc gtgctcgccc gagcgcatca gcgcgagctc gacctggtcc
     3241 tggatgtctt ctatatggaa ggtgccgccg gtcggctggc gccggatgag cgcgccgacc
     3301 accgcctcgg tcagcccgga cacgatgtcg cggatacgcg ccgagccggc tccctgggtg
     3361 ccgttcaccg ccaggaaggc cttggtcatg gccaccgaga tcttggccgg ctcgaagccg
     3421 accaccgcgc cgttgcgccg gatgatcttg tagtcggcgt aggggctgcg cccggtactc
     3481 gactcaggaa tggcgacgcc caaggcctgg tttgcggtgg cgttttcctg agcgatttgc
     3541 atgtcaccgt ctcccgtgtg tctgactgcg ttgccgggac cgtccgccgc ttttgtatat
     3601 tgcggttctg tgacgacctt acctatcacc tacaacatct agtgcaactg ctctattcgt
     3661 ctacaagatg taggtcggag cctacacacg aaaaaatttt tccgcaagcg tgcggacgcc
     3721 ttacagccac tagatattgg atgcggaggg gggacgaccg ctacatattg ggtactaaag
     3781 gcgtgcctgc tgcactgcca caaagcatgc gtactacata gccgggataa cctggaaagc
     3841 agtcctacat ctagtaggtg tcggcaggct gccagaccta caacaacttc aggcttgcgc
     3901 caggtccgcc agcaaccgcg cccagtcgaa atgcggcccc ggatcggtct tgcgccccgg
     3961 cgcgacttcg ctgtgcgcgg cgatatcgcg caaggaaaga gaagcgcgca ggcagcgcgc
     4021 gagcgccgca agtgtggaat attgcacgcc ggcgaaggcc tcttcctcgc tgccttccag
     4081 ttcgatgccg atcgaaaagt cgttgcagcg ggaacgtccg cgccaggccg aggcgccggc
     4141 gtgccaggcg cgctcatgca ccggaacgaa ttgcagcaat gcaccgtccc ggcgcaccag
     4201 aaaatgagac gagacttgca aacaaaagac ttccctgaaa tacggatgcg cgtcggggtc
     4261 cagggtgttg gtgaagaggc gctcgatggc gtcgccgccg tactcgccgg gcggcaggct
     4321 gatggcgtgc agcagaagca acgagatctc gctgccctcc ggccgctcgt cccggttcgg
     4381 cgaaggcacg cgccgggcgt cctccagcca accgtccggc cccagcttca tgctgcgggc
     4441 ttctcctcgc ggatgcccag gcgctgcatg cggtagcgca gctggcggaa ggtgatgccc
     4501 agcagcttcg ccgcggcggt gcggttgaag ccggtcttgg cgagggcctc gaggatcgcc
     4561 ttgcgctcca ggccgtccag gtagtcgggc agcgattcga cgctcgggcc ggcgctgtcg
     4621 cgatcggcct cgagccgctg cggctcgagg cgcagatcct cgacgccgat ctcctcggcg
     4681 ccggagagcg ccaccgcacg ctccagcacg ttctccagct cgcggatgtt gccgggaaaa
     4741 tcgtagcgcg ccagctcctc gagcgcccgc ggcgagaggc gcgccgggcc gccgccggcc
     4801 tggccgccca ggcgcgccag gatggccgcc gcgatgagcg cgatgtcctc gcggcagtcg
     4861 cgcagcggcg gcatcaccag ctcgatcacg ttcaggcggt agaagagatc ctggcggaag
     4921 cggccggcgg ccaccagcgc gccgaggttc tggtgggtgg cgcagatgat gcgaacgtcg
     4981 accggatcct cctgcgtcgc gccgaccttg cgcacgcgct tttcctggat cgtgcgcagc
     5041 agcttcacct gcatcggcag cggcagctcg gcgacttcgt ccaggaacag cgtgccgccg
     5101 ttcgccgcct ggaagaagcc gtcgcgatcc tcccccgccc cggtaaacgc gcccttcttg
     5161 tagccgaaga actcgctttc catgagggtc tcggggatgg cgccgcagtt gaccggcacg
     5221 aaaggctgct cgcgccgcgc gccgttgtcg tggatcaggc gcgcggcgag ctccttgccg
     5281 ctgcccgact cccccgagat gtagaccggg gcctgggtgc gggcgagctt gccgatcatc
     5341 tcgcgcacca gcacgagcgg ctggctcgcg ccgaggagct gctgcccctt ggccggcgcc
     5401 atgacgcgat cgggcagcga gagcgccgat ttcaccagcg cgcgcagctg ctccaggccg
     5461 accggcttgg agacgtagtc gaaggcgccg gccttgagcg cggccaccgc gttctcggcg
     5521 ctgccgtagg cggtgatcac cgccaccggc aggtccgcgc ccaggccggc gatgtggcgc
     5581 accaggtcga gcccctcgcc gtcggcgagg cgcatgtcgg tcaggcaaag cgcgtagcgc
     5641 tcctcgccct tcagcagctc cttggcctcg gcgatcgagc cgacgctcgc cacccccagg
     5701 cccatcttga ccagcgtcag ctcgagcagc tcgcggatgt cgggctcgtc gtcgaccacc
     5761 agcacctgcg cgccacgcgg gccggagcgg cgttcagcgc gcttcatgcc gccctcgcct
     5821 cgcggcacag gatcctgaaa tgggcgccgg gcaggtcgtc gacatactcg aggatggcgc
     5881 ggttcgcgct gcacagctcg cgcgccaggt acaggcccag gccggtgccc ttgctctcgg
     5941 tggtgaagaa gggctcgaac agctgcccct ggtcggcggc cggcacgccc ggcccgttgt
     6001 cgatcacgct caa
//
LOCUS       DASYJA010000403         5061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2510494, whole genome shotgun sequence.
ACCESSION   DASYJA010000403 DASYJA010000000
VERSION     DASYJA010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2510494"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1539)
                     /locus_tag="VGO02_08875"
     CDS             complement(1..1539)
                     /locus_tag="VGO02_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477833.1"
                     /translation="MAARYLLYFSTEDHYLYRSVRGELELEATFSDDEAGVGALREFL
                     RGRKGALVSILADLSGEDFHEEQIPLLRGADREAVVQRRLAQRYRDTRLAAALSLGMV
                     LAGERRNERLLLASFTNTQSLAPWLDALEESDARLAGVFSVPLLAPALAARLGARSGR
                     AFVVTATRAGLRQSFVEDGKLRFARLERTIEMTPQALAMFVRSETLRLAQYLVTLRVL
                     PREAGPVQALVVAPPGQRAAFEQVLQSDTRLVFRTLDATEAEAAVGLRRKPAGTAGEA
                     LYLHLAAKNPPREQFASSTERHRYLVWRLQRTVLAAGAIGFAACALFAGAKWLDVLQA
                     RNRAELQAQEARRSAEAYARITSSFPVTQTSSENLKVAVVEFTRIAQRASAPERILAH
                     VSRALEQFPQFEIDAISWAAGRPGERRDARGAPPKPEAQAETAETVEVSGRVNATQKS
                     DYRGITAQVQRFAEALGSSGYQLTRTQLPFDITSEGTLTGDIGGAETGEAPRFTIVVT
                     RRLP"
     gene            complement(1546..2751)
                     /locus_tag="VGO02_08880"
     CDS             complement(1546..2751)
                     /locus_tag="VGO02_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV7477834.1"
                     /translation="MPLFTYRAIDAQGKSVPGRIEAINLFDLEQRLSRMGLDLVNGAP
                     TSQRTRLIGGSRVSRQDLINFCFHLEQLATAGVPVLEGLNDLRDSVENPRFREVVSGL
                     VESIEGGRNLSGALAEYPEVFSKVFVSLVRSGEQTGKLPEVLKSLSESLKWEDELAAQ
                     TKKILMYPAFVGSIVLLVTFFLMIYLVPQMTGFIRNMGQEIPLQTLVLIAVSDFFVNF
                     WWAVMAAPFVAWAGLKFAKSYNPAVQYAIDHYKISAPLIGPILRKIILSRFASSFAMM
                     YASGITVLDAIRGCEEIVGNKPLENALRTAGQQIAEGKNMTAAFADLGLFPPLIIRML
                     KIGESTGALDTALLNVSYFYNREVRESIGKVQAMIEPALTLVLGALLGWVMLSVLGPV
                     YDAISKMKF"
     gene            complement(2756..4465)
                     /locus_tag="VGO02_08885"
     CDS             complement(2756..4465)
                     /locus_tag="VGO02_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEV7477835.1"
                     /translation="MNSPANPQAKRHIGQILIDQGILTEDQLRIALLEQTKSHVPVGR
                     LLVQLGFVSEATLRDALSEKLGLQSVDLAHIIVDPAALKALPREMARRYRIFPVALDR
                     QQKKFIVALADTNNIVAIDQLRAHLKGEFEIELRLAGDSEIERAVEHYYGHEFSIDGI
                     LREIETGEVDYTTITEGDEYSQPVVRLISALLADAVERAASDIHFEPEQNFLRIRYRI
                     DGVLRQIRSLHKTYWPAMAVRLKVMAKMNIAETRAPQDGRISATMAGRTVDFRVASLP
                     TTHGENLVLRILDRQKGIVPLEKLGLEESQLELLKRMLARPEGIIRVTGPTGSGKTTT
                     LYSVLNHINSDAINIMTLEDPVEYPMTLIRQTSVAESAKLDFANGIRALMRQDPDAIL
                     VGEIRDRDTAEMAFRAAMTGHQVYSTLHTNSAVGAFPRLLDIGIVPDILSGNIIGIIA
                     QRLVRKLCRTCRQSYDPDVRERRLLGITPTEPAALFRAVGCEQCDYQGYRGRGAILEI
                     LKIDGGIDELIARRATAREILTEARAKGFKTLADDGTRLVRSGSTSLDELMRVVDLTD
                     RMT"
     gene            complement(4546..>5061)
                     /locus_tag="VGO02_08890"
     CDS             complement(4546..>5061)
                     /locus_tag="VGO02_08890"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV7477836.1"
                     /translation="AKARGEYEQALADEPGNRDALLGLAAVDTRSGRYEAAEAHYLRL
                     LQVDPRDAHAQAGLFSLRSARLDPLASESRAKSMLAQDPGSNVLNFALGNQLAQQGRW
                     AEAQQEYFKAFAGDPENADFAYNLAVSLDHLRQARLARDYYLRAAALAEKRGASFDPA
                     TARLRAGQLAN"
BASE COUNT          733 a         1705 c         1818 g          805 t
ORIGIN      
        1 tcatggcagc cgccgcgtca cgacgatggt gaagcgcggc gcttcgcccg tctcggcgcc
       61 gccgatgtcg ccggtcagcg tgccctcgga ggtgatgtcg aacggcaact gggtgcgggt
      121 gagctggtag ccggaggagc cgagcgcctc ggcgaagcgc tgcacctggg cggtgatccc
      181 ccgataatcg ctcttctgcg tggcgttgac gcggcccgag acctcgactg tctccgccgt
      241 ctcggcctgc gcctcgggct tgggcggcgc accgcgcgcg tcgcgccgct cgcccggccg
      301 cccggccgcc cagctgatcg cgtcgatctc gaattgggga aactgctcga gcgcgcgcga
      361 aacgtgcgcc aggatccgct cgggcgccga ggcgcgctgc gcgatgcgcg tgaactcgac
      421 caccgccacc ttgaggttct cgctcgaggt ctgcgtcacc gggaagctcg acgtgatgcg
      481 ggcgtaggct tccgccgagc ggcgcgcctc ctgcgcctgc agctcggcgc ggttgcgcgc
      541 ctgcaggacg tcgagccatt tcgccccggc gaacagcgcg caggcggcga agccgatggc
      601 gccggcggcg agcaccgtgc gctgcagccg ccagacgagg tagcggtgcc gctcggtgct
      661 cgaggcgaac tgctcgcgcg gcgggttctt cgccgcgagg tgcaggtaca gggcctcgcc
      721 ggcggttccc gccggcttgc gccgcaggcc gacggctgct tcggcttcgg tggcgtccag
      781 cgtgcggaac acgaggcggg tgtcggactg cagcacctgc tcgaacgcgg cacgctgccc
      841 cggcggggcc accaccagcg cctggaccgg cccggcctcg cgcggcagga cccgcagcgt
      901 caccaggtac tgcgcgaggc gcagcgtctc cgagcgcacg aacatggcga gcgcctgcgg
      961 cgtcatctcg atggtgcgct ccaggcgcgc gaagcgcagc ttgccgtcct cgacgaagct
     1021 ctggcgcagg ccggcgcgcg tcgcggtgac gacgaacgcc cggccgctgc gcgcgccgag
     1081 ccgcgcggcg agcgccggcg cgagcagcgg caccgagaac acgccggcca gcctggcgtc
     1141 ggactcctcc agcgcgtcga gccacggcgc gaggctctgc gtgttggtga aggaggcgag
     1201 cagcagccgc tcgttgcgcc gctcgccggc cagcaccatg cccagcgaca gcgccgcggc
     1261 gaggcgcgtg tcgcggtagc gctgcgccag gcgccgttgc acgacggcct cacggtcggc
     1321 gccgcgcagc agcgggatct gctcctcgtg gaagtcctcg ccggacaggt cggccaggat
     1381 ggagaccagg gcgcccttgc gcccgcgcag aaactcgcgc agggcgccga cgccggcctc
     1441 gtcgtcgctg aacgtcgcct cgagctcgag ctcgccgcgc acgctgcggt agaggtagtg
     1501 gtcctcggtc gagaagtaga ggaggtagcg cgcagccatg ctcgcctaga atttcatctt
     1561 cgagatcgcg tcgtacaccg gcccgagcac cgacagcatc acccagccga gcagcgcgcc
     1621 gagcaccagc gtgagcgccg gctcgatcat cgcctgcacc ttgccgatcg actcgcgcac
     1681 ctcgcggttg tagaagtagc tgacgttgag cagcgccgtg tcgagcgccc cggtgctctc
     1741 gccgatcttc agcatgcgga tgatgagcgg cgggaacagg cccaggtcgg cgaacgccgc
     1801 ggtcatgttc ttgccctcgg cgatctgctg gcccgcggtg cgcagcgcgt tctccagcgg
     1861 cttgttgccg acgatctcct cgcagccgcg gatcgcgtcg agcacggtga tgcccgaggc
     1921 gtacatcatg gcgaagctcg aggcgaagcg cgacaggatg atcttgcgca ggatcgggcc
     1981 gatgagcggc gccgagatct tgtagtggtc gatggcgtac tgcaccgccg ggttgtagct
     2041 cttggcgaac ttgaggcccg cccaggcgac gaacggcgcg gccatgaccg cccaccagaa
     2101 attgacgaag aagtccgaca ccgcgatcag caccagagtc tgcagcggga tctcctggcc
     2161 catgttgcgg atgaagccgg tcatctgcgg caccaggtag atcatcagga agaaggtcac
     2221 cagcagcacg atgctgccga cgaaggccgg gtacatgagg atcttcttgg tctgcgccgc
     2281 gagctcgtct tcccacttca gcgactccga caggctcttc agcacctcgg gcagcttgcc
     2341 ggtctgctcg cccgagcgca ccaggctgac gaacaccttg ctgaatacct cgggatactc
     2401 ggcgagcgcg cccgacaggt tgcgcccgcc ctcgatcgac tcgaccaggc ccgacacgac
     2461 ctctcgaaag cgtggatttt ccacgctatc gcgcagatcg ttcagcccct cgagcaccgg
     2521 cacgccggcg gtggcgagct gctcgagatg gaagcagaag ttgatcaggt cctggcgcga
     2581 gacgcggctg ccgccgatca ggcgcgtgcg ctggctcgtc ggcgcgccgt tgaccaggtc
     2641 gaggcccatg cgcgagagcc gctgctcgag gtcgaacagg ttgatcgcct cgatgcggcc
     2701 cggaacgctt ttcccctggg cgtctatcgc tcggtaggta aacagcggca ttgcatcagg
     2761 tcatccggtc ggtgaggtcg acgacgcgca tcagctcgtc gaggctggtg gagcccgagc
     2821 gcaccaagcg cgtgccgtcg tcggccagcg tcttgaagcc cttggcgcgc gcttcggtga
     2881 ggatctcgcg cgccgtcgcg cgccgcgcga tcagctcgtc gatgccgccg tcgatcttga
     2941 ggatctcgag gatcgcgccg cgcccgcggt agccctggta gtcgcactgc tcgcaaccca
     3001 ccgccctgaa cagggccgcc ggctcggtcg gcgtgatgcc gagcaggcgc cgctcgcgca
     3061 cgtccgggtc gtaggactgg cggcaggtgc ggcacagctt gcgcaccagg cgctgcgcga
     3121 tgatgccgat gatgttgccc gacaggatgt cgggcacgat gccgatgtcg agcaggcgcg
     3181 ggaaggcgcc gaccgcggag ttggtgtgca gcgtcgagta gacctggtgg ccggtcatgg
     3241 cggcgcggaa cgccatctcc gccgtgtcgc ggtcgcggat ctcgccgacc aggatcgcgt
     3301 cggggtcctg gcgcatcagg gcgcggatgc cgttggcgaa gtcgagcttg gccgactcgg
     3361 ccaccgaggt ctggcggatc agcgtcatcg ggtactcgac cgggtcctcg agcgtcatga
     3421 tgttgatcgc gtcggagttg atgtggttga ggaccgagta gagcgtggtg gtcttgccgc
     3481 tgccggtcgg gccggtcacc cggatgatgc cctcggggcg cgccagcatg cgcttgagca
     3541 gctcgagctg cgactcctcc aggccgagct tctccagcgg cacgatgcct ttctggcggt
     3601 cgaggatgcg cagcaccagg ttctcgccgt gcgtggtcgg caggctggcg acgcggaagt
     3661 cgaccgtgcg ccccgccatg gtcgccgaga tgcgcccgtc ctgcggcgcg cgcgtctcgg
     3721 cgatgttcat cttcgccatg actttcagtc gcaccgccat cgccggccag taggtcttgt
     3781 gcagcgagcg gatctggcgc agcacgccgt cgatccgata cctgatgcgc aggaagttct
     3841 gctcgggctc gaagtgaata tcggacgcgg cccgctcgac cgcgtccgcg aggagcgccg
     3901 aaatgaggcg caccaccggc tgcgagtact cgtcgccctc ggtgatggtg gtgtagtcca
     3961 cctcgccggt ctcgatctcg cgcaggatgc cgtcgatcga gaactcatgg ccgtagtagt
     4021 gctcgaccgc gcgctcgatc tccgaatcgc cggccaggcg cagctcgatc tcgaactcgc
     4081 ccttcaggtg ggcgcgcagc tggtcgatgg cgacgatgtt gttggtgtcg gcgagcgcga
     4141 cgatgaactt cttctgctgg cggtcgagcg ccaccgggaa gatgcggtag cggcgcgcca
     4201 tctcgcgcgg cagcgccttc agcgccgccg ggtcgacgat gatgtgcgcc aggtcgaccg
     4261 actgcaggcc gagcttctcg gacagcgcgt cgcgcagcgt cgcttccgac acgaagccga
     4321 gctgcaccag caggcgcccg accgggacgt gcgacttggt ctgctcgagc agcgcgatcc
     4381 tcagctggtc ctcggtgagg atgccctggt cgatcaggat ctggccgatg tggcgcttgg
     4441 cctggggatt cgcgggcgaa ttcatccgga taggatgata accggggggg tacgccgggg
     4501 ggtgtcaact ttgcccatga gcgtggggcc catggtcaga ttgtgtcagt tcgccagctg
     4561 acccgcccgg aggcgggccg tcgcgggatc gaagctcgcg ccgcgcttct ctgcgagcgc
     4621 ggcggcgcgc agatagtaat cgcgcgcgag gcgcgcctgg cgcaggtggt cgaggctgac
     4681 cgccaggttg taggcgaagt ccgcgttctc cgggtcgccg gcgaatgcct tgaaatattc
     4741 ctgctgcgcc tcggcccagc gaccctgctg cgcgagctgg ttgccgaggg cgaagttcag
     4801 cacgttcgag cccgggtcct gcgcgagcat cgacttcgcg cggctctcgg aggcgagcgg
     4861 gtcgaggcgc gcgctgcgca gggagaacag cccggcctgc gcgtgcgcat cgcgcggatc
     4921 cacctgcagc agccgcaggt agtgggcctc cgccgcctcg tagcggccgc tgcgcgtgtc
     4981 gacggcggcc aggccgagga gcgcgtcgcg gttgcccggt tcgtcggcga gcgcctgctc
     5041 gtattcgccg cgcgccttgg c
//
LOCUS       DASYJA010000404         3218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1784190, whole genome shotgun sequence.
ACCESSION   DASYJA010000404 DASYJA010000000
VERSION     DASYJA010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1784190"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1540
                     /locus_tag="VGO02_08895"
     CDS             <1..1540
                     /locus_tag="VGO02_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEV7477837.1"
                     /translation="TVTDERLFEVIASTPAFQEGGIFSKTAYVNLLRSLNPPMTPSQY
                     EARVRHDLALQQLAMAVGAAIPSRTVSERVAAIESQKREVSEARIASQQFLPQAKIDD
                     AKLKEYYEANAAEFRRPERVRAEYIVLSADQLARNEQVSEADLKAAYQQRLAQMTDAE
                     QRRASHILVKTKDEAEKINLEIKKNPNRFADLAKKHSQDTGSAEKGGDLGWFGRGMMV
                     KPFEDAVFAMKQGETSAPVQSDFGFHVIRVTGVKAAKARSFEEARAEIEAELKKSRAA
                     KRFNEVADAFQNMVYEQADSLKPAAERFKLPVQSTGWIVKSSAQELGALDNPKLVAAL
                     FSPDAIQSKRNTDAIEVAPATLVSARVVEHQPAAQRSFDEVKQELAESLRKREAVALA
                     QKEGMAKLELLRKGGDAGVSWGKPRTVSRRDAKGMAPELVRQVVAADVSKLPAYVGAP
                     AQEGGYVLLRISKVTDAEPAEKSPDSGQRMAATLGAAQYQAYVSSLRSRASIEVRSLS
                     PEKK"
     gene            complement(1521..2327)
                     /gene="fabI"
                     /locus_tag="VGO02_08900"
     CDS             complement(1521..2327)
                     /gene="fabI"
                     /locus_tag="VGO02_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEV7477838.1"
                     /translation="MGFLAGKKILVTGLLSNRSIAFGVAKAARREGAELALTYQNERF
                     KDRAADMAKELGAQVVLPCEVTSDAEIQGVFTELKKHWDGLDGLVHAIAFAPREAIAG
                     DFLEGLSRAAFAQAHDISVYSFPALAKAALPMMQGRRAALVTLTYLGAARVVPNYNTM
                     GLAKASLEASVRYLAASLGPKGIRVNGISAGPIKTLAAAGISGFGKILKFVEEHSPMR
                     RNVSTEDVGNAAAFLLSDLAAGITGEILYVDSGFSHVVAGLGGEATSSPG"
     gene            2349..>3218
                     /locus_tag="VGO02_08905"
     CDS             2349..>3218
                     /locus_tag="VGO02_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7477839.1"
                     /translation="MRIFLILLLFLAACGDAWNDPYPAGEHGKSILYSAFTERPKHLD
                     PVQSYTEDEARFTQQVYEPPLQYHYLKRPYELIPLTALEIPKARVIQGGKFTEYEVRI
                     RPGIKYQPHPAFVSENQKLKSATIEKLKTPYELPLGTRELTADDYIYQIKRLAHPRLH
                     SPILGLMSEYIVGLDDLSKQLKNKTSSGWLDLRETPLKGVERVDAHTFRVRLNGSYPQ
                     FVYWLAMPFFSPVPWEAEKFFEQPGMAEKNFTLDWWPVGTGPYMLTENNPNARMLLER
                     NPNFHGETYPAEGE"
BASE COUNT          611 a         1045 c         1079 g          483 t
ORIGIN      
        1 caccgtgacc gacgagaggc tgttcgaggt gatcgcgtcg accccggcat tccaggaggg
       61 cgggatattc tcgaagaccg cgtacgtcaa cctgctgcgc tcgttgaatc cgcccatgac
      121 gccctcacag tacgaggcgc gcgtgcgcca cgacctcgcc ctgcagcagc tcgccatggc
      181 ggtgggcgcg gcgatcccgt cgcgcaccgt gtccgagcgc gtcgccgcga tcgagtcgca
      241 gaagcgcgag gtgtccgagg cgcgcatcgc ctcgcagcag ttcctgccgc aggctaagat
      301 cgacgacgcg aagctgaagg aatattacga ggcgaatgcc gccgagttcc gccgcccgga
      361 gcgcgtgcgc gccgagtaca tcgtgctctc ggccgaccag ctggcgcgca acgagcaggt
      421 gagcgaggcc gacctgaagg cggcctacca gcagcgcctg gcgcagatga ccgacgccga
      481 gcagcgtcgc gccagccaca tcctggtcaa gacgaaggac gaagcggaaa aaatcaattt
      541 ggaaatcaaa aaaaatccaa accggttcgc cgacctggcc aagaagcact cgcaggatac
      601 gggctcggcg gagaagggcg gcgacctcgg ctggttcggc cgcggcatga tggtcaagcc
      661 gttcgaggac gcggtgttcg cgatgaagca gggcgagacc agcgcgccgg tgcagagcga
      721 cttcggcttc cacgtcatcc gcgtcaccgg cgtgaaggcc gccaaggcgc gctccttcga
      781 ggaagcgcgc gccgagatcg aggccgagct gaagaagagc cgcgccgcca agcgcttcaa
      841 cgaggtggcc gacgccttcc agaacatggt gtacgagcag gccgacagcc tcaagccggc
      901 cgccgagcgc ttcaagctgc cggtgcagag caccggctgg atagtgaagt cgtccgcgca
      961 ggagctcggc gcgctcgaca acccgaagct ggtcgccgcg ctgttctcgc ccgacgcgat
     1021 ccagagcaag cgcaacaccg atgccatcga ggtggcgccg gcgacgctgg tatcggcgcg
     1081 ggtggtcgag caccagcccg cggcgcagcg cagcttcgac gaggtgaagc aggagctggc
     1141 cgagtcgctg cgcaagcgcg aggcggtggc gctggcgcag aaggagggca tggccaagct
     1201 cgagctgctg cgcaagggcg gcgatgccgg cgtgtcctgg ggcaagccgc gcacggtatc
     1261 gcggcgcgac gcgaagggga tggcgccgga gctggtgcgc caggtcgtgg cggccgacgt
     1321 gagcaagctg ccggcctacg tcggcgcgcc ggcgcaggaa ggcggctacg tgctgctgcg
     1381 catctcgaag gtgaccgacg cggagcccgc cgagaagtcg cccgactcgg gccagcgcat
     1441 ggcggccacg ctgggcgcgg cgcagtacca ggcgtacgtg tcgagcctgc gcagccgcgc
     1501 cagcatcgaa gtgagaagcc tcagcccgga gaagaagtag cctcgccgcc cagcccggcg
     1561 acgacgtggc tgaagccgga atcgacgtac aggatctcgc cggtgatgcc ggccgccagg
     1621 tccgagagca ggaaagccgc cgcgttgccc acatcctcgg tgctgacgtt gcgccgcatg
     1681 ggcgagtgct cctcgacgaa cttcaggatc ttgccgaagc cgctgatgcc ggcggcggcg
     1741 agcgtcttga tcgggccggc cgagatgccg ttgacgcgga tacccttcgg gccgaggctc
     1801 gcggcgaggt agcgcaccga ggcttccagg ctcgccttcg ccaggcccat cgtgttgtag
     1861 ttcggcacca cgcgcgcggc gccgagatag gtgagggtga cgagcgcggc gcgccgcccc
     1921 tgcatcatcg gcagcgctgc cttggcgagc gccgggaagc tgtagaccga gatgtcgtgc
     1981 gcctgggcga aggccgcgcg cgacagccct tcgaggaagt cgccggcgat cgcctcgcgc
     2041 ggcgcgaagg cgatggcgtg caccaggccg tcgaggccat cccaatgctt tttcaactca
     2101 gtaaacactc cctgaatttc agcgtcgctc gtgacctcac aggggagaac aacctgtgca
     2161 ccgagctcct tcgccatgtc ggcggcacgg tccttgaagc gctcgttctg gtaggtcagg
     2221 gcgagctcgg cgccttcgcg gcgcgcggcc ttcgccacgc cgaaggcgat ggaccggttg
     2281 ctgagcagcc cggtgacgag aatctttttg ccggctagga atcccatccc accgattata
     2341 ctgtccgcgt gcgcatcttc ctgatcttgc tgttgtttct agcggcttgt ggtgacgcct
     2401 ggaacgatcc gtatcccgcc ggcgagcacg gcaagagcat tctgtacagc gccttcaccg
     2461 agcgccccaa gcacctcgac ccggtgcagt cctataccga ggacgaagcg aggttcacgc
     2521 agcaggtcta cgagccgccg ctgcagtacc actatctgaa gcgcccgtac gagctgattc
     2581 cgctcaccgc gctcgagata ccgaaggcgc gcgtgatcca gggcggcaag ttcacggagt
     2641 atgaagtgcg catccgcccg ggcatcaaat accagccgca ccccgcattt gtttctgaaa
     2701 atcagaaact gaagagcgca acgattgaaa agctgaaaac cccatatgag ctcccgcttg
     2761 ggacgcgcga gctcacggcc gacgactaca tctaccagat caagcgcctt gcccatccgc
     2821 gcctgcattc gccgatcctc ggcctgatga gcgaatacat cgtcggcctc gatgatcttt
     2881 caaagcaatt aaaaaacaag acgagtagcg gctggctcga tctgcgtgag acccccctga
     2941 agggcgtgga gcgcgtcgac gcccatacct tccgggtcag gctgaacggc agctatccgc
     3001 aatttgtcta ctggctggcg atgccgtttt tctcgccggt gccgtgggag gctgagaagt
     3061 tcttcgagca gcccggcatg gcggagaaga acttcacgct cgactggtgg ccggtcggca
     3121 ccgggcctta catgctgacc gagaacaacc cgaacgcgcg catgctgctc gagcgcaacc
     3181 cgaatttcca cggcgagacc tatcccgccg aaggcgag
//
LOCUS       DASYJA010000405         3472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2783703, whole genome shotgun sequence.
ACCESSION   DASYJA010000405 DASYJA010000000
VERSION     DASYJA010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2783703"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VGO02_08910"
     CDS             <1..451
                     /locus_tag="VGO02_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV7477840.1"
                     /translation="GLELALGCHYRIAAPGTNVALPEVKLGLIPGAGGTQRLPRAVGK
                     AKAMDMCLTGRMMDAQEAERAGLVSRVVPLERLMEEAFAAAEKIAGYSLPVAMMIKES
                     INRAYESSLAEGVRVERRVFHSQFALEDQKEGMAAFVEKRKPNFKNK"
     gene            489..1634
                     /locus_tag="VGO02_08915"
     CDS             489..1634
                     /locus_tag="VGO02_08915"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7477841.1"
                     /translation="MAGFSTFAALLPELRDLWGLSNSQAGIVGGTFFAGYIATVSFWS
                     ALTDRLDARKVYLAGGLLSAAGGAGFGLAADGFLSAVLFQCLLGIGIAATYMPGLRLL
                     SDRISGPGQSRYIAFYTSFFGIGTALSLAMAGALAPAFGWRAAFVASAVGPVMASLLV
                     WFFIGGIEPKAPSSRALFPVAAWRKVLADRASAGYTFGYFAHCLELFGSRAWMVAFLV
                     FSATKNEGFPWAPAAIAAVVNLLSVPASIFGNEIALRIGRRRWILIAMAGSGLTGMLV
                     GLSAPWHWALVLALIVLYSMLVMAESATLTAGLVAAAPAELRGAAMGLYSLIGFGGGM
                     LGPVVFGAALDAAGGAQASLAWVAAYAAIGAGCLAAPLVSRMNKRPL"
     gene            1643..3307
                     /locus_tag="VGO02_08920"
     CDS             1643..3307
                     /locus_tag="VGO02_08920"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEV7477842.1"
                     /translation="MDRIWLKSYPPGVPADIDPNEYKSLVELFEAGIRKYAARPAFTN
                     MGKTITYGELDGLSRAFGAWLQAKGLGKGARVALMMPNCLQYPVAMFGTLRAGCTVVN
                     VNPLYTPRELEHQLKDSGAEAIVILENFGHVLQEVRAKTPLKHIVVTSLGEMLGLKGL
                     LVNLVVRKVKKMVPAFELPGCVGFKEVIDSGKEKKLITPALGHADIAYLQYTGGTTGV
                     SKGAILSHRNIVSALLQYRAWLGPGMGERPVIITALPLYHIFSLTVNCLVMMVVGGEN
                     VLITNPRDIPGFVKELARHKYTMITGVNTLFNALLNHPDFAKLDFSRVKVAIGGGMAV
                     QKAVAERWKQVTGTTLIEGYGLTETAPSATANPLDLAAFSGSIGVPMPSTEIALRDDA
                     DKDVPLGQPGEICIRGPQVMAGYWQQPAETAKVLGKDGYLHTGDIGVMDDKGFIRIVD
                     RKKDMILVSGFNVYPNEIEQVVAMHPGVLEVAAIGVPDAHSGEVPKVFVVKKDPGLTE
                     ADILEHCKKELTGYKRPKYVEFRTELPKTNVGKILRRALREEKKAA"
BASE COUNT          558 a         1158 c         1183 g          573 t
ORIGIN      
        1 cggactggaa ctggcgctgg gttgccatta ccgcatcgcc gcacccggca ccaacgtggc
       61 cttgcccgaa gtgaagctgg gcctgatccc cggcgccggc ggcacgcagc gcctgccgcg
      121 cgcggttggc aaggcgaagg cgatggacat gtgcctcacg gggcgcatga tggacgcgca
      181 ggaggccgag cgcgccggcc tggtgtcgcg cgtggtgccg ctcgagcggc tgatggagga
      241 agcgttcgcc gcggcggaga agatcgccgg ctattcgctg cccgtcgcca tgatgatcaa
      301 ggagtcgatc aaccgcgcct acgagagctc gctcgccgag ggcgtgcgcg tcgagcggcg
      361 cgtgttccat tcgcagttcg cgctggaaga ccagaaagag ggcatggccg ccttcgtcga
      421 aaaacgcaag ccgaacttca aaaataaata agaccccgtc ggtcctgctg ggcgcccaca
      481 tcctgtcgat ggcgggcttc tccaccttcg ccgcgctgct gcccgagctg cgcgacctgt
      541 ggggcctgtc caactcgcag gccggcatcg tcggcggcac cttcttcgcc ggctatatcg
      601 ccacggtctc cttctggagc gcgctcaccg accggctgga cgcgcgcaag gtgtacctcg
      661 cgggcgggct gctctcggcc gcgggcggcg ccggcttcgg cctggccgcc gacggcttcc
      721 tcagcgccgt attgttccag tgcctgctcg gcatcggcat cgcggcgacc tacatgcccg
      781 ggctgcggct gctttccgac cgcatctccg gtcccggcca gagccgctat atcgccttct
      841 acacgtcctt cttcggcatc ggcaccgcgc tctcgctcgc catggcgggc gcgctcgcgc
      901 ccgcattcgg ctggcgcgcg gccttcgtgg cctccgccgt gggaccggtg atggccagct
      961 tgctggtctg gtttttcatc ggtgggattg aacccaaagc gccttcatcg agagccctct
     1021 ttcccgtggc ggcgtggcgc aaggtgctcg ccgaccgggc atcggccggc tacaccttcg
     1081 gctatttcgc gcactgcctc gagctcttcg gctcgcgcgc ctggatggtc gcgttcctgg
     1141 ttttttccgc gacgaaaaac gaaggctttc cgtgggcccc cgccgccatc gccgcggtgg
     1201 tgaacctgct ctccgtgccg gcgtcgatct tcggcaatga gatcgcgctg cgcatcggcc
     1261 ggcggcgctg gatcctgatc gccatggcgg gctcgggcct caccggcatg ctggtcgggc
     1321 tttccgctcc ctggcactgg gcgctggtgc tcgcgctgat cgtgctctat tcgatgctgg
     1381 tgatggccga gtcggcgacg ctcaccgccg gcctggtggc ggcggcgccg gccgagctgc
     1441 gcggcgcggc gatgggcctg tattcgctga tcggcttcgg cggcggcatg ctcgggccgg
     1501 tggtgttcgg ggccgcgctc gacgccgcgg gtggcgccca ggcgtcgctc gcatgggtgg
     1561 cggcctacgc cgcgatcggc gccggctgct tggccgcacc gctcgtgagt agaatgaata
     1621 aaaggcccct ataggaggag gcatggaccg catctggctc aagagctatc cccccggcgt
     1681 tcccgccgac atcgacccga acgagtacaa gtcgctggtc gagctgttcg aggcgggcat
     1741 ccgcaagtac gccgcgcggc ccgccttcac caacatgggc aagacgatca cctacggcga
     1801 gctcgacggg ctgtcgcgcg ccttcggcgc ctggctgcag gcgaaggggc tgggcaaggg
     1861 cgcgcgcgtc gcgctcatga tgcccaactg cctgcagtac ccggtggcga tgttcggcac
     1921 gctgcgcgcc ggctgcacgg tggtgaacgt caatccgctc tacaccccgc gcgagctcga
     1981 gcaccagctg aaggactccg gcgccgaggc gatcgtgatc ctggagaatt tcggccacgt
     2041 cctgcaggag gtgcgcgcga agaccccgct gaagcacatc gtcgtcacct cgctgggcga
     2101 gatgctcggc ctgaagggcc tgctggtgaa cctggtggtg cgcaaggtga agaagatggt
     2161 gccggcgttc gagctgccgg gttgcgtcgg cttcaaggag gtgatcgatt ccggaaaaga
     2221 aaaaaagctg atcacgcccg ctctggggca cgccgacatc gcgtatctgc aatacaccgg
     2281 cggcacgacc ggcgtctcca agggcgcgat cctgagccac cgcaacatcg tctcggccct
     2341 gctgcagtac cgggcctggc tcggtccggg gatgggcgag cggccggtga tcatcaccgc
     2401 gctgccgctc taccacatct tctcgctgac ggtgaactgc ctggtgatga tggtggtcgg
     2461 cggcgagaac gtgctcatca ccaacccgcg cgacataccg gggttcgtga aggagctcgc
     2521 caggcacaag tacacgatga tcaccggcgt gaacacgctg ttcaacgcgc tgctcaacca
     2581 cccggacttc gccaagctcg actttagccg cgtgaaggtg gcgatcggcg gcggcatggc
     2641 ggtgcagaag gcggtggccg agcgctggaa gcaggtgacc ggcaccacgc tgatcgaagg
     2701 ctacggcctg accgagaccg cgccgtcggc gaccgccaac ccgctcgacc tggcggcctt
     2761 cagcggctcg atcggcgtgc cgatgccttc caccgagatc gcgctgcgcg acgacgccga
     2821 caaggacgtg ccgctcggcc agccgggtga gatctgcatc cgcggcccgc aggtgatggc
     2881 cggctactgg cagcagcccg ccgagaccgc caaggtgctc ggcaaggacg gctacctgca
     2941 caccggcgac atcggcgtga tggacgacaa gggcttcatc cgcatcgtcg accgcaagaa
     3001 ggacatgatc ctggtgtcgg gcttcaacgt gtacccgaac gagatcgagc aggtggtggc
     3061 gatgcatccc ggggtcctcg aggtggcggc gatcggcgtg cccgacgcgc actcgggcga
     3121 ggtgccgaag gtgttcgtgg tgaagaagga cccgggcctc accgaggccg acatcctcga
     3181 gcactgcaag aaggagctca ccggctacaa gcggccgaaa tacgtcgagt tccgcaccga
     3241 gctgccgaag accaacgtcg gcaagatcct gcgccgcgcg ctgcgcgagg aaaaaaaagc
     3301 ggcctagtgc cgccccggaa acagaaggcc acccaggaag gttggttgaa acgaggaatt
     3361 tcgattagca cattgctgct cgccgtggtc ttcgcgcagg gttgcgccac caagcactac
     3421 ggccgccagg atgccctcac gccctacgag aaagacaagc tcacgtgccg gg
//
LOCUS       DASYJA010000406         4518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1149516, whole genome shotgun sequence.
ACCESSION   DASYJA010000406 DASYJA010000000
VERSION     DASYJA010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149516"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VGO02_08925"
     CDS             complement(<1..382)
                     /locus_tag="VGO02_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEV7477843.1"
                     /translation="MNTMTAQNPMLPLLARVLIGALFVTAGARKALTIAATAGYFAKL
                     GFPAPEVLAYLAILIEVGGGLALILGWQTRWIAWLLTIFVVIATGMAHRFWEFDAAQY
                     NAQLNNFLKNVAIIGGLLMFATYGP"
     gene            434..1630
                     /locus_tag="VGO02_08930"
     CDS             434..1630
                     /locus_tag="VGO02_08930"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7477844.1"
                     /translation="MQRRYDRVIVAGGGPVGSLAALLLARAGVPVTLLEREAGVQLDY
                     RASTFHPPTLDLLEECGAEAALLRMGLKCTTSQFRDRMKGKIAEFDLGALRRDTRHPY
                     RLQCEQFKLVDWLHGELRRVPGVELLFEKNITHMEADEDGVAVHVESGEAFHCDYLIG
                     ADGGRSAVRKAAGIAFEGFTFPEHFLVAGTRYDFKRHMPDICSVNYTADPVEWYLLLE
                     IPDMWRVITAADPAIEPDEAVQDARLQASLQNLLPRSEPYEILVKGVYRVHQRVAAAY
                     RLGRVFLAGDAAHLNNPLGGMGLNGGLQDVLSLVPRLVDVWRGEKPDAELGGYEAQRR
                     PEAIHAINAITARNKKLMEERDPAVRERNLRQMTEIASDPRRAYDYMLEASMITSLRR
                     CGMLPT"
     gene            complement(1623..2135)
                     /locus_tag="VGO02_08935"
     CDS             complement(1623..2135)
                     /locus_tag="VGO02_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEV7477845.1"
                     /translation="MKCLKAVVAGATLAIAASASAQQPNPMDNVPDKMPNDIPYGAPI
                     SLERAQAAINAAVAESKKRGWKLNVAMVDSGGHLVAFARMDGAQLGSIAISEHKARAA
                     AKFRRETKAFEAGVQAGNNYLLTLDDVIASRGGIPVLQNGQIIGAIGCSGGTGSQDEV
                     VCKAGAEAVK"
     gene            complement(2160..2561)
                     /locus_tag="VGO02_08940"
     CDS             complement(2160..2561)
                     /locus_tag="VGO02_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEV7477846.1"
                     /translation="MKTRPMLTLDDCKKISAAAEAEAKKNSWNVCIAILDDGGHLLHL
                     ARMDGASPANARIAIAKGRTAAESRRSTQVWEDRIKAGRNSMLGMPGITPVQGGLPIM
                     VEGTCVGGIGASGVQSHEDEQIVGAGIKALG"
     gene            complement(2558..4426)
                     /locus_tag="VGO02_08945"
     CDS             complement(2558..4426)
                     /locus_tag="VGO02_08945"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HEV7477847.1"
                     /translation="MKYSEFHRRSLDDRDAFWTEQAKLIDWHKPFGKVLDYSRPPFAR
                     WFVGGETNLCHNALDRHLEARGAQKALIYVSTETGEERSYSYKELFREVNQAAAMMRS
                     LGVKQGDRVIVYMPMIPEALFSMLACARLGAVHSVVFGGFAAASLAARIDDARPALMV
                     TADGGSRMGKVVTYKSLVDEALNLAKHPPKKVLIFKRGLDPSMPVVSGRDVDWKELSK
                     GQTGNIDCVWLESSEPSYILYTSGTTGRPKGVQRDTGGYAVALAASMKHIFCGEPGET
                     MFTTSDIGWVVGHSYIIYAPLIAGMTTIMYEGVPIRPDAGIWWKIVQDHKVAVMFSAP
                     TAIRVLKKQDPAYLKKYDLSSLKHLFLAGEPLDEPTHVWISQALGRPVIDHYWQTETG
                     WPLLSAHPGVEKLPLKMGSPSFPAYGYDVRLLHEGSGKEVKDGEKGVVTVTPPLPPGC
                     MSTLWGDDQRFVNTYFKDFKGQQVYSTFDWGIRDKDGYYFILGRTDDVINVAGHRLGT
                     REIEEAVSMHPNVAECAVVGVADQIKGQMPLAFAVLKDTSKATSSQEVMETVDRQLGA
                     IARPKAVYFVTLLPKTRSGKTLRRSIQAVAEGRDPGDLTTIEDPNALEQIRKALHS"
BASE COUNT          750 a         1476 c         1460 g          832 t
ORIGIN      
        1 cgggcccgta ggtggcgaac atcagcaggc cgccgatgat ggcgacgttc ttgaggaaat
       61 tgttcagctg ggcgttgtac tgggcggcgt cgaactccca gaagcggtgc gccatgccgg
      121 ttgcgatgac gacgaagatc gtaagcagcc aggcgatcca gcgcgtctgc cagccgagga
      181 tcagcgccag tccgccgccg acctcgatca agatcgcgag gtaggccaag acttcgggtg
      241 ccgggaagcc gagcttggcg aaatagccgg cggtggcggc gatggtcaag gctttgcggg
      301 cgccggcggt gacgaacagc gcaccgatca ggacgcgggc cagcagcggc aacatcggat
      361 tttgcgcagt catggtattc atggtcccct cctccttgtg gcggcgatta tgctccatac
      421 tcgctaggct cgcatgcagc gcagatacga ccgcgtcatc gtggcgggcg gcggccccgt
      481 cggctcgctt gctgcgctgc tcctggcgcg cgccggcgtg ccggtcacgc tcctcgagcg
      541 cgaggccggg gtgcagctcg actaccgcgc ctccaccttc cacccaccca cgctcgacct
      601 gctcgaggaa tgcggcgccg aggccgcgct gctgcggatg ggtctcaagt gcaccaccag
      661 ccagttccgc gaccggatga aaggcaaaat cgccgagttc gacctcggcg cgctgcggcg
      721 cgacacacgc cacccctatc gcctgcaatg cgagcaattc aagctggtcg attggctgca
      781 tggggagctg cgtcgcgtcc ccggtgtgga attgttgttt gagaaaaaca taacccacat
      841 ggaagccgat gaagatggcg tagcggtgca tgtcgaaagc ggagaagctt tccactgcga
      901 ctacctgatc ggcgctgacg gcgggcgcag cgcggtgcgc aaggctgcgg gcatcgcctt
      961 cgagggcttc accttccccg agcacttcct ggtcgcgggc acgcgctacg acttcaagcg
     1021 ccacatgccc gatatctgct cggtcaacta cacggcagat ccggtcgagt ggtacctgct
     1081 gctcgagatc ccggacatgt ggcgcgtgat cacggcggcc gacccggcca tcgagcccga
     1141 cgaggcggtg caggacgctc gcctgcaggc cagcctgcag aacctgctgc cgcgctccga
     1201 gccctacgag atcctggtga aaggcgtcta ccgggtgcac cagcgagttg ccgccgccta
     1261 tcgcctcggg cgcgtgttcc tcgccggcga cgcggcgcac ctcaacaatc cgctgggcgg
     1321 catgggcctg aacggcgggc tgcaggacgt gctgagcctg gtgccgcggc tggtcgacgt
     1381 gtggagggga gaaaagccgg atgccgagct cggcggctac gaggcgcagc ggcggcccga
     1441 ggcgatccac gccatcaatg ccatcacggc gcgcaacaag aagctgatgg aggagcgcga
     1501 tccggcggtg cgggagcgca atctccgcca gatgacggag attgcgtccg acccgcgccg
     1561 cgcctacgac tacatgctcg aagcgtcgat gatcacgtcg ctccggcgct gcgggatgct
     1621 gcctacttga cggcttcggc gcccgccttg cagaccacct cgtcctgcga gccggtgccg
     1681 ccggagcagc cgatggcgcc gatgatctga ccgttctgca gcacggggat gccgccacgc
     1741 gaggcgatca cgtcgtcgag ggtaagcagg tagttgttgc cggcctgcac gcccgcctcg
     1801 aacgccttgg tctcgcggcg gaatttcgcc gcggcgcgtg ccttgtgctc ggagatggca
     1861 atcgagccga gctgcgcacc gtccatccgg gcgaaggcga ccaggtgccc gcctgaatcg
     1921 accatggcca cgttcagctt ccagccgcgc ttcttcgact cggccaccgc ggcgttgatc
     1981 gccgcctggg cgcgctcgag cgagatcggc gctccatagg ggatgtcgtt cggcatcttg
     2041 tcgggcacgt tgtccatcgg attgggctgc tgcgctgacg cgctggctgc gatggcgagc
     2101 gtagcgcccg ccactactgc tttcaggcat ttcacgttct ttctcctcaa aaaatcgggt
     2161 taaccgagcg ccttgattcc tgcgccgaca atctgctcgt cttcgtgcga ctgcacgccg
     2221 gaggcaccga tgcctccgac gcaggttcct tcgaccatga tgggcaggcc gccctgcacc
     2281 ggggtgatgc ccggcatgcc gagcatggaa ttgcgcccgg ccttgatgcg atcttcccat
     2341 acctgggtcg agcggcgcga ctcggcggcg gtgcgccctt tcgcgatcgc gatgcgtgcg
     2401 ttggccggcg aggcgccgtc catgcgcgcc aggtgcagca ggtgcccgcc gtcgtcgagg
     2461 atcgcgatgc acacgttcca gctgttcttc ttcgcttccg cctcggcggc ggcagagatc
     2521 ttcttgcaat cgtcgagggt cagcatcggt cttgtcttca tgagtgcagg gcctttctga
     2581 tctgttccaa agcgttggga tcttcgatgg tggtgaggtc gcccgggtcg cgtccttccg
     2641 cgaccgcctg gatcgagcgg cgcagcgtct tgccggaccg ggtcttgggc agcaaggtca
     2701 cgaaatacac tgctttcggc cgcgcgatcg cccccagctg cctgtctacc gtctccatga
     2761 cctcctggga cgaggttgcc ttcgatgtgt ccttcaagac ggcaaacgca agaggcatct
     2821 gacccttgat ctggtccgct acgccgacca ccgcgcactc ggcgacgttc gggtgcatgc
     2881 tcaccgcctc ttcgatctcg cgcgtgccga ggcggtgccc cgcgacgttg atcacgtcgt
     2941 cggtgcggcc gaggatgaaa tagtagccgt ccttgtcgcg aatgccccag tcgaaggtcg
     3001 aatagacctg ctgccctttg aagtccttga aataggtatt cacgaagcgc tggtcgtcgc
     3061 cccacagcgt cgacatgcag cccggcggca gcggtggagt gaccgtgact acgccttttt
     3121 ccccatcctt gacttctttt ccagaacctt catgaagtaa acgcacgtca tagccgtagg
     3181 ccggaaagct cggcgagccc atcttcagcg gcagcttctc cactcccgga tgagccgaga
     3241 gcagcggcca gccggtctcg gtctgccagt agtgatcgat caccggcctg ccgagcgcct
     3301 ggctgatcca gacgtgcgtc ggctcgtcga gcggctcgcc ggcgaggaac agatgcttga
     3361 gcgaggacag atcgtatttc ttcaggtacg cgggatcctg cttcttgagc acgcggatgg
     3421 cggtaggcgc cgagaacatc acggctacct tatggtcctg cacgatcttc caccagatgc
     3481 cggcgtcggg ccggatcggc acgccttcgt acatgatggt cgtcataccc gcgatgagcg
     3541 gtgcgtaaat gatgtacgaa tggcccacca cccagccgat gtccgaggtg gtgaacatcg
     3601 tctcgcccgg ctcgccgcag aagatgtgct tcatcgacgc cgccagcgcc accgcatagc
     3661 caccggtgtc gcgctgcacg cccttcggcc gcccggtggt gcccgaggta tagaggatgt
     3721 aagacggctc gctcgattcg agccatacgc aatcgatatt cccggtttgc cctttggaca
     3781 gttccttcca gtccacgtcg cgtccactga cgacgggcat cgaggggtca aggcctctct
     3841 tgaaaatcag caccttcttc ggcggatgct tcgccaggtt gagcgcctcg tctacgagcg
     3901 acttgtaggt cactaccttg cccatgcggc tgccgccgtc ggcggtgacc atcagcgcgg
     3961 gcctggcgtc gtcgatgcgc gctgcgaggc tggctgcggc gaagcctccg aacaccaccg
     4021 agtgcaccgc gccgaggcgc gcgcaggcga gcatcgagaa cagcgcctcg gggatcatcg
     4081 gcatgtatac gattacccgg tcaccctgct tcacgccgag cgagcgcatc atcgccgcgg
     4141 cctggttcac ttcgcggaac agctccttgt atgaatagga tctttcttcg ccggtctccg
     4201 tcgagacgta gatcagggcc ttctgcgcgc cgcgcgcctc gaggtggcgg tcgagcgcgt
     4261 tgtggcagag gttggtctcg ccgccgacga accacctggc gaacggcggg cgcgaatagt
     4321 cgagcacctt gccgaacggc ttgtgccaat cgatcagctt cgcctgctcg gtccagaacg
     4381 cgtcgcggtc atccagagaa cggcgatgga actcggaata cttcacttct tgcccttcag
     4441 gcgctcgagc acgggtatca ggcgccgaaa tacctggccg gcgcccgcac cgagccgctc
     4501 cggtatgagg cgctcgag
//
LOCUS       DASYJA010000407         1968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1785169, whole genome shotgun sequence.
ACCESSION   DASYJA010000407 DASYJA010000000
VERSION     DASYJA010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1785169"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VGO02_08950"
     CDS             <1..237
                     /locus_tag="VGO02_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477848.1"
                     /translation="PLLLPAIIAGWLMLFVIFIRELGATILLYAQGTETISVALVVLS
                     EHSIGRVAALAVIQLILLLFAFTLFRATRASLLG"
     gene            248..1363
                     /locus_tag="VGO02_08955"
     CDS             248..1363
                     /locus_tag="VGO02_08955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7477849.1"
                     /translation="MAFLSITNLRKRFDGLIAVDGASLEVEEGHTLALLGPSGCGKTT
                     ILRCLAGLETADEGAIEIAGKPVFDHAAGVNLMPEQRELGIVFQSYAVWPHMSVAENV
                     AFPLMVRGVPKQETKERTARILELVGLTAAGHKGATALSGGQQQRVALARALIHEPRL
                     VLFDEPMSNLDAQLRAQMRMELGVLQDRLGFTAIYVTHDQAEAFALAETVAVMNQGRI
                     ETVGEPRRVFHRPATPFVAQFLGLNVLRGRVVAAQGEFSRVALADGVEIWGSAASPLA
                     VGTAALLCVRKEHVAVRRAAAAAAAPATVQPPAEQVFPAEIRTASFLGLQEEYIVAIG
                     GVELGSIQPPHGLRAGERVEAAIRPADCVLFADASAG"
     gene            1406..>1968
                     /locus_tag="VGO02_08960"
     CDS             1406..>1968
                     /locus_tag="VGO02_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477850.1"
                     /translation="MRRSLSYLFVAAPAGLIASGAGAQEPPALQGVSAQEKSRVTALI
                     DAAKQEGALTYWDVVIQPETNDALAAEFRKTYGLPSSFQVNYQLSATANLVTRVEQEI
                     AANRVTIDIAAIGSPSWVFERANKGDALEYDSPQYKFFEQAFKNGLGLPKFFAFNGAY
                     LFVPMWDGAKTKFAGKSWKDVLGAVPAG"
BASE COUNT          304 a          672 c          675 g          317 t
ORIGIN      
        1 ccgctgctgc tgccggcgat catcgcgggc tggctgatgc tgttcgtgat cttcatccgc
       61 gagctgggcg ccaccatcct gctctatgcc cagggcaccg agaccatcag cgtcgcgctg
      121 gtggtgctgt ccgagcacag catcggacgg gtggcggcgc tcgcggtgat ccagctcatc
      181 ctgctgctgt tcgccttcac gctgttccgc gcgacgcgcg cgtcgctcct gggttgagga
      241 gcgcgccatg gccttcctgt cgatcacaaa cctgcgcaag cgttttgacg gcctgatcgc
      301 cgtcgacggc gcctcgctcg aggttgagga gggccacacg ctcgccctgc tcggcccgtc
      361 gggctgcggc aagaccacga tcctgcgctg cctcgccggg ctcgagaccg cggacgaggg
      421 cgcgatcgag atcgccggca agccggtgtt cgaccatgct gccggcgtca acctgatgcc
      481 cgaacagcgc gagctcggca tcgtgttcca gtcctatgct gtgtggccgc acatgagcgt
      541 cgccgaaaac gtggcgttcc cgctcatggt gcgcggcgtg cccaagcagg aaaccaagga
      601 gcgcacggcg cgcatcctcg agctcgtcgg cctcacggcg gcggggcaca agggcgcgac
      661 cgcgctctcg ggcggccagc agcagcgcgt ggcgctcgcc cgcgcgctca ttcacgagcc
      721 gcggctggtg ctgttcgacg agccgatgtc caatctcgac gcgcaattgc gcgcccagat
      781 gcgcatggag ctcggcgtgc tgcaggaccg gctcggcttc accgcgatct atgtcaccca
      841 cgaccaagcg gaagccttcg cgctggccga aacggtcgcg gtcatgaacc agggccggat
      901 cgagacggtg ggcgagccac gccgggtctt ccaccggccg gcgacgccct tcgtggcgca
      961 attcctcggg ctcaatgtgc ttcgcggccg cgtggtggcg gcgcagggcg aattttcccg
     1021 ggtcgcgctc gccgacggcg tcgaaatatg gggcagcgcc gcaagccccc tggcggtggg
     1081 cacggcggcg ctgttgtgcg tgcgaaaaga gcatgtcgcg gtgcgcaggg cggcggcggc
     1141 ggcagccgcg cccgccaccg tccagccgcc ggccgagcag gtgtttccgg ccgagatccg
     1201 gacggcctcg ttcctcggcc tgcaggagga atacatcgtc gccatcggcg gcgtggagct
     1261 cggctcgatc cagccgccgc acggcctgcg cgccggcgag cgcgtcgagg cggcgatcag
     1321 gcccgcggac tgcgtccttt ttgcagatgc ctcggctggc tgagccggta agatcatgtc
     1381 gctggaattc aataggagaa ggccgatgcg ccgaagtctg tcatacctgt ttgtcgcggc
     1441 ccccgcgggc ctgatcgcga gcggcgccgg cgcgcaggag ccaccggccc tgcagggcgt
     1501 gtcggcgcag gagaagtcgc gggtgaccgc tctgatcgac gccgccaagc aggaaggcgc
     1561 gctgacctat tgggacgtcg tcatccaacc cgagaccaac gacgcgctgg cggcggaatt
     1621 ccgcaaaacc tacggactgc cgagctcgtt ccaggtcaac taccagctgt ccgccaccgc
     1681 caacctggtc acccgcgtcg agcaggagat cgcggccaac cgcgtcacca tcgacatcgc
     1741 ggcgatcggc tcgccctcat gggtgttcga gcgcgccaac aagggcgacg cgctggaata
     1801 cgattcgccg cagtacaaat tcttcgagca ggcgttcaag aacggcctcg gcttgccgaa
     1861 attcttcgct ttcaacggcg cctatctgtt cgtgccgatg tgggacggcg ccaagaccaa
     1921 gttcgcgggc aaatcctgga aggacgtgct gggcgccgtc ccggccgg
//
LOCUS       DASYJA010000408         1684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_606009, whole genome shotgun sequence.
ACCESSION   DASYJA010000408 DASYJA010000000
VERSION     DASYJA010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606009"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1655)
                     /locus_tag="VGO02_08965"
     CDS             complement(<1..1655)
                     /locus_tag="VGO02_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477851.1"
                     /translation="MTRLEQQLAFARESETRITQQVAQLTEQIGALAGQDQALLGDRA
                     AVETAIDDALSAREATGSEERAAAAALPLLERAVAEKAAVLAELQQQVALAEQTIRVI
                     ETRRENLGRVQAQLAERRDRLNGELAALAAPMTAKIADVEDQHRQETAELATREAGLN
                     ALRDAVQGLQDRQRTASDAAQQAGTTLADIAARAEALSALQAKIGQGKDSTQWLDDKG
                     LASARRLWQGLDIEPGWEDALEAVLRERLNAVELPRLEVARDWHDARPAGAGGPPRVA
                     VYERGSGTAGDAGAGTDALYARVRRVGPEIARLLADWLRGVRCRDDVAVAIRDRHALA
                     VGESYVTPQGHLVTAQSISFFAPDSDLHGVLARQRELGELEHRIGSAREAAQAARAAL
                     SAVEAELDDAQQRYHAESMAFASQQRRCHDLELELLQLTQAAEVAAKRRVQIDAELAE
                     VAAQEEAEHAQEAALARELADAQLRLHDEAAKRDGARHGQNEAEVEFARGRERVRVAE
                     RVAQEAAFAERASRERLAELERRREALAAQTAQQQALLTQLTS"
BASE COUNT          181 a          643 c          578 g          282 t
ORIGIN      
        1 tcggaggtca gttgcgtcag cagtgcctgc tgctgcgcgg tctgcgccgc cagcgcttcg
       61 cgccgtcgtt cgagctccgc gagccgctcg cgactcgcgc gctcggcgaa cgcagcctcc
      121 tgcgccacgc gctcggcgac gcgtacgcgc tcgcggccgc gcgcaaattc gacctccgcc
      181 tcgttctgac cgtggcgcgc gccatcgcgc ttcgcggcct catcgtgcag gcgcagctgc
      241 gcatcggcga gctcgcgcgc gagcgccgcc tcctgcgcgt gctcggcctc ctcctgcgcc
      301 gcgacctcgg cgagctcggc gtcgatctgc acgcggcgct tcgcggcgac ttcggcggcc
      361 tgcgtgagct gcagcagctc gagctcgaga tcgtggcatc gccgctgctg cgacgcgaag
      421 gccatgctct ccgcgtggta gcgctgctgc gcgtcgtcca gctcggcctc gactgccgac
      481 agcgcggcgc gtgccgcctg ggcggcctcc cttgcgctgc cgattcgatg ctcgagctct
      541 cccaactcgc gttggcgtgc gagcacgcca tgcaagtcgc tatccggcgc gaaaaagctg
      601 atcgactgcg cggtgacgag gtggccctgc ggggtcacgt acgactcgcc caccgcgagc
      661 gcatgcctgt cgcgaatcgc cacggcgacg tcgtcgcggc agcgcacgcc gcgaagccag
      721 tcggccagga gccgcgcgat ctccggtccc acgcggcgca cgcgtgcata gagcgcgtcc
      781 gtgccggcgc ctgcgtcgcc cgcagttccg ctgccgcgct cgtagacggc gacgcggggc
      841 ggtccgcccg ctcctgccgg ccgcgcatcg tgccagtcgc gcgcgacctc cagacgcggg
      901 agctcgaccg cattgaggcg ctcgcgcagc actgcctcga gcgcgtcctc ccacccgggc
      961 tcgatgtcga ggccctgcca caggcggcgg gcgctcgcga gtcccttgtc gtcgagccac
     1021 tgcgtgctgt ccttgccctg gccaatcttc gcctgcagcg cggagagtgc ctcggcgcgc
     1081 gcggcgatat cggcgagcgt cgttcctgcc tgctgcgcgg catcgctcgc ggtgcgctgc
     1141 cggtcctgca atccctgcac ggcgtcgcgc agcgcgttca ggcccgcttc gcgcgtcgcg
     1201 agctcggccg tttcctgacg gtgctgatcc tcgacatcgg cgatctttgc cgtcatcggc
     1261 gccgcgaggg ccgcgagctc gccgttgagt cggtcgcggc gctccgcaag ttgcgcctgc
     1321 acgcgaccca ggttttcgcg ccgggtttcg atcacgcgga tcgtctgctc ggcgagcgcg
     1381 acttgttgct ggagctcggc gagcaccgcg gccttttccg cgacggcacg ctcgagcagc
     1441 ggcagtgccg ccgccgccgc acgttcctcc gaacccgttg cctcgcgcgc ggacagcgca
     1501 tcgtcgatcg ccgtttcgac cgcggcgcgg tcgccgagga gcgcctggtc ctgtccggcc
     1561 agggcaccga tctgctcggt gagttgcgcg acctgctgcg tgatccgcgt ctcgctctcc
     1621 cgggcgaatg cgagctgctg ctcgaggcgc gtcacctcgg cgttcgcttc gtagaaagcg
     1681 ccct
//
LOCUS       DASYJA010000409         2748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2694291, whole genome shotgun sequence.
ACCESSION   DASYJA010000409 DASYJA010000000
VERSION     DASYJA010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2694291"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..848
                     /locus_tag="VGO02_08970"
     CDS             99..848
                     /locus_tag="VGO02_08970"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEV7477852.1"
                     /translation="MHEGINAAALELALAGRATAISCLVGAPSWRHGVSRLREVDPRA
                     VDVGLHLDLTGFPLHAPRRGLPWLIAAALSRQLDELALRDEVRRQLDAFEYALGRAPS
                     HVDGHQHVHQLPQVREVLVAALVERHGRLPWLRDTRRPAGEARPKPWLIEQLGASGLR
                     RLARTAGLAQNAHLLGVYDFSDSPAAYEAHLVRWLREARPGDLLMCHASAACDAPDPI
                     IAARRNEHRTLSSAWFTEQLAKEGLTIARLG"
     gene            complement(850..1347)
                     /locus_tag="VGO02_08975"
     CDS             complement(850..1347)
                     /locus_tag="VGO02_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV7477853.1"
                     /translation="MSKPFKPDPKLDLVLERVVDVPPALVWACWTMPKHMKKWFTPAP
                     WKTVEVEVDLRPGGRLYFVMQSPEGENFPNTGCFLEVAKNERLVWTNALLPGYRPASS
                     GAASADDLTFTAVITMKKQGKGTKYTALAIHRNEDEAQKHAKMGFHDGWGKALDQLVA
                     LAKKM"
     gene            complement(1344..1682)
                     /locus_tag="VGO02_08980"
     CDS             complement(1344..1682)
                     /locus_tag="VGO02_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV7477854.1"
                     /translation="MPNQSADLGRVFQALADPTRRAVLERLAGGPAPMSELAQPFSMA
                     LPSFSQHLDVLEDCGLVSSSKDGRVRTYRIAPRAMKAAEGWLAKQRVLWGRRLDQLDD
                     YLDELKEQKR"
     gene            complement(1747..1947)
                     /locus_tag="VGO02_08985"
     CDS             complement(1747..1947)
                     /locus_tag="VGO02_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477855.1"
                     /translation="MKAKLICSAILALASFNTFAQDAAADANDPAVVCRVLVCDKSVS
                     ASEAAFERSMLVFQQRRQLGLN"
     gene            complement(2159..2353)
                     /locus_tag="VGO02_08990"
     CDS             complement(2159..2353)
                     /locus_tag="VGO02_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477856.1"
                     /translation="MKTELILIAILAMAASLSAFAQDAAQDLNQPGRWSTPAPERAAA
                     DEYTGVGLQEGLCTVSQRKI"
     gene            complement(2363..>2748)
                     /locus_tag="VGO02_08995"
     CDS             complement(2363..>2748)
                     /locus_tag="VGO02_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477857.1"
                     /translation="MASFGSLAQDKTASCIDSLAADSRLQVIADKVALARSGQASPMR
                     ALDRVANDQERAAVAVWFGRRQECFEAGSRKRRALSTPQEIAFLRSVFVFQQRLVGDL
                     QDGRLTYAEFNQRRGELLQAAGKEI"
BASE COUNT          404 a          939 c          929 g          476 t
ORIGIN      
        1 gatcgccgcc accccggaga tggtcctctg gcgctatgaa ctcgtatcgg cacccggtga
       61 atgcaagaac cttcacagtc ggcattgatg atttcggctt gcatgaggga atcaatgccg
      121 cggcgctgga gctggcccta gccggccgcg caacggcgat cagttgcctg gtaggcgcgc
      181 cgtcctggcg gcacggcgtg tcgcgcctgc gcgaagtcga tccgcgcgcc gtggacgtag
      241 gcctgcacct cgacctcacc gggtttcccc tgcacgcgcc gcgccgcggc ctgccctggc
      301 tgatcgcggc cgccttgagc cgccagctcg acgagctggc gctgcgcgac gaggtccggc
      361 gccagctcga tgccttcgaa tatgcgcttg gacgcgctcc gagccacgtg gacgggcacc
      421 agcacgtcca ccagctgccg caggtgcgcg aagtgctcgt cgccgccctc gtcgagcgcc
      481 atgggcgcct gccctggctg cgcgacacga ggcgaccggc tggagaggcg cgcccgaagc
      541 cctggctgat cgagcagctc ggcgcctccg gcctgcggcg gctggcgcgg accgccgggc
      601 tcgcgcaaaa cgcgcatctg ctcggtgtct acgacttctc ggactcgccg gcagcctacg
      661 aagcgcacct cgtgcgctgg ctgcgcgaag cgcgccccgg cgatctgctg atgtgccacg
      721 ccagcgcggc ttgcgacgcg ccggacccga tcatcgcggc ccgccgcaac gagcaccgca
      781 cgctgtcgtc cgcgtggttc acagaacagc tggcgaagga aggcttgacg atcgcccgtc
      841 tcggctgacc tacatcttct tggcgagcgc gacgagctgg tcgagcgcct tgccccagcc
      901 gtcgtggaag cccatcttcg cgtgcttctg cgcctcatcc tcgttgcggt ggatcgccag
      961 cgcggtgtac ttcgtgccct tgccctgctt cttcatggtg atgacggccg tgaaggtgag
     1021 gtcgtcggcg ctcgcggcgc cgctgctggc cgggcggtag ccgggaagca gcgcgttggt
     1081 ccacaccaga cgctcgttct ttgcgacctc gaggaaacag ccggtgttcg ggaagttttc
     1141 accctccggg gactgcatga cgaagtacag gcggccgccg ggtcgcaggt cgacctcgac
     1201 ctcgaccgtc ttccagggcg ccggggtaaa ccacttcttc atgtgctttg gcatcgtcca
     1261 gcaggcccag accagagccg gcggcacgtc gacgacgcgc tccaggacga ggtccagctt
     1321 gggatcgggt ttgaacggct tgctcatctc ttctgctcct tgagttcgtc gaggtagtcg
     1381 tcgagttggt cgaggcggcg cccccaaagc acgcgctgct tcgccagcca gccttcggcc
     1441 gccttcatgg cccgcggggc gatgcggtag gtgcgcaccc ggccatcctt gctcgagctc
     1501 accaggccgc agtcctccag cacgtcgagg tgctgcgaga aggacggcag cgccatgctg
     1561 aaaggctgcg ccagctcgct catgggcgcg gggccgccgg cgagccgctc gagcaccgcg
     1621 cgccgggtag ggtcggcgag cgcctggaag acgcgtccga ggtcggccga ttggttaggc
     1681 atgtgcctaa ctatacgcgc aaataaaaag ggccgctctc gcggcccctc ttagatgtct
     1741 agagtgctag ttcaggccga gctgccgccg ctgctggaac accagcatgc tgcgctcgaa
     1801 cgcggcttcc gacgcgctga cgctcttgtc gcacaccagc acgcggcaga ccaccgccgg
     1861 gtcgttggcg tcggcggcgg cgtcctgggc gaaggtattg aagctggcca gggccaggat
     1921 cgcgctgcag atcagtttcg ctttcatctc tatctccctg ccggcaaacc aggccgccgg
     1981 tctgtgagtg catttgaagc caacgcgcag cgcgcgccta taggggatcg gtcaggactt
     2041 gtgcgactgg acacagcctc acaggagtgt cgaatcgacg acgattgtcg gagaaccgac
     2101 aaaaaaaagg gccgctcacg cggcctcttg ttattgtccc ccgggtattc cccccggttc
     2161 agatcttccg ttgcgacacc gtgcaaaggc cttcctgcag ccccacaccg gtgtactcgt
     2221 cggcggcggc ccgctcgggc gccggcgtgc tccaccggcc cggctggttg aggtcctgcg
     2281 cggcgtcctg ggcgaacgcg ctcaggctgg cggccatggc gaggattgcg atgaggatca
     2341 gttcggtttt catgttttct ccctagatct ctttgccggc ggcctggagc agctcgccgc
     2401 ggcgctggtt gaactcggca taggtcaggc ggccgtcctg caggtcgccc accaggcgct
     2461 gctggaagac gaagacgctg cgcagaaagg cgatctcctg cggggtcgac agggcgcgcc
     2521 gtttccggct gccggcctcg aagcactcct ggcgcctgcc gaaccacacc gcgacggcgg
     2581 cgcgctcctg gtcgttcgcc acgcggtcga gcgcgcgcat cggggaggcc tggccgctgc
     2641 gggcgagcgc caccttgtcg gcgatcacct gcaggcggct gtcggccgcc agcgagtcga
     2701 tgcagctcgc ggtcttgtcc tgggcgaggc tgccgaagct ggccatcg
//
LOCUS       DASYJA010000410         3015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_606237, whole genome shotgun sequence.
ACCESSION   DASYJA010000410 DASYJA010000000
VERSION     DASYJA010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606237"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2296
                     /gene="mfd"
                     /locus_tag="VGO02_09000"
     CDS             <1..2296
                     /gene="mfd"
                     /locus_tag="VGO02_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV7477858.1"
                     /translation="GLRVLVAAESPGRRETMLTYFAEYGLTPDPAGNFSDFLSSSAKF
                     AITVAPLAHGFAVPAEGWAIVTEAELYAGVVRRRGRAAEKRASVEGMLRDLSELKVGD
                     PVVHEQHGIGRFQGLVSLNLDEGQSEFLLLEYEGGDKLYVPVAQLGVIGRYSGAQPEE
                     APLHKLGSGQWDKAKARAARQVRDAAAELLALYARRAARLGFAFSVDQKSLEAFADGF
                     GFEETPDQASAIEAVVQDMAAGKPMDRLVCGDVGFGKTEVALRAAFVAVADKKQVAVL
                     CPTTLLAEQHFQTFGDRFADWPVELAELSRFRSQKQISESLKKLESGEIDIAIGTHKL
                     LGADIKFSRLGLVIIDEEHRFGVRQKEALKKLRAEVDVLTLTATPIPRTLALSLEGIR
                     DFSVIATAPERRLAIKTFVQPFSEGLIREAVLRELKRGGQIYFLHNEVDSIERMAERL
                     RRLLPEARLAIAHGQMRERELERVMRDFTAQRCNVLLCSTIIETGIDIPTANTIVIDR
                     ADKFGLAQLHQLRGRVGRSHHQAYAYFLTPPHDALNKNAIKRLEAIQMMEELGSGFYL
                     AMHDLEIRGAGEVLGESQSGEIQEIGFSLYARMLDRAVRRLKSGKDIDWEGSVDVSTE
                     VNLHVPALLPASYCSDVHERLSLYKRLADADSREALDLLREELVDRFGDLPEPARALL
                     ECHQVRIAARALGVARVDATHEAVQLQFVKNPPLDGAKVIAFIKRRSGARLTGQDRLR
                     VEMKLPAWQERGQAVQDILKQLAA"
     gene            2321..>3015
                     /gene="serB"
                     /locus_tag="VGO02_09005"
     CDS             2321..>3015
                     /gene="serB"
                     /locus_tag="VGO02_09005"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEV7477859.1"
                     /translation="MNLILQSPTLERGDVDAIADGVRPSSMEKLSARAFRLKGATPHD
                     SVAKLAEARRIDYAWMEPGRRFADLKLLAMDMDSTLITIECIDELGAIAGKGAEIAAI
                     TEAAMRGEIADYKDSLRRRVSLLAGQPAGILESVYQSKLRLTPGAEQLLDACKRHGVK
                     TLLVSGGFTFFTGRLKDRLRLDYTISNTLQTKAGSLTGALLGDIVDADAKAAKFVAVM
                     KELKAGKQQTVAIG"
BASE COUNT          528 a         1015 c         1031 g          441 t
ORIGIN      
        1 cggcctgcgc gtgctggtgg cggccgagtc gcccggccgg cgcgagacga tgctcacgta
       61 tttcgccgag tacggcctga cgccggaccc tgccgggaat ttttccgact ttctttcgtc
      121 aagcgccaaa ttcgccatca cggtcgcgcc gctcgcgcac ggcttcgccg tccccgccga
      181 gggctgggcg atcgtcaccg aagccgagct ctacgccggc gtggtgcggc gccgcggccg
      241 cgccgccgag aagcgcgctt cggtcgaggg catgctgcgc gacctgtcgg agctcaaggt
      301 cggcgacccg gtggtgcatg agcagcacgg catcggcaga ttccagggac tggtcagtct
      361 caatctggac gaagggcaaa gcgagttcct gctcctcgag tacgaaggcg gcgacaagct
      421 ctacgtgccg gtggcccagc tcggcgtcat cgggcgctac agcggcgcgc agcccgagga
      481 agcgccgctg cacaagctcg gcagcgggca atgggacaag gcgaaggcgc gcgccgcaag
      541 gcaggtgcgc gacgccgcgg ccgagctgct cgcgctgtac gccaggcgcg cagccagatt
      601 gggttttgct ttttcagtcg atcaaaaatc cctggaggcc ttcgccgatg gcttcggttt
      661 cgaggaaacg cccgaccagg catccgccat cgaggcggtg gtccaggaca tggccgccgg
      721 caagccgatg gaccggctgg tgtgcggcga cgtcggcttc ggcaagaccg aggtcgcgct
      781 gcgcgccgcc ttcgtcgcgg tcgccgacaa gaagcaggtg gcggtgctct gccccaccac
      841 gctcctcgcc gagcagcact tccagacctt cggcgaccgc ttcgccgact ggccggtgga
      901 gctcgccgag ctgtcccgct tcaggagtca gaaacagata tccgaatcgt tgaaaaagct
      961 ggaaagcggc gagatcgaca tcgccatcgg cacccacaag ctgctcggag ccgacatcaa
     1021 gttctcgcgc ctggggctgg tgatcatcga cgaggagcac cgcttcggcg tgcggcagaa
     1081 ggaggcgctg aagaagctgc gcgccgaggt cgacgtcctc acgctcaccg cgacgccgat
     1141 cccgcgcacc ctggcgctgt cgctggaggg gatccgcgac ttctcggtga tcgccaccgc
     1201 gccggagcgg cgcctcgcca tcaagacctt cgtgcagccc ttctcggagg gcctgatccg
     1261 cgaggcggtg ctgcgcgagc tgaagcgcgg cggccagatc tacttcctgc acaacgaggt
     1321 cgactcgatc gagagaatgg cggaaaggct gcggcgcctg ctgccggagg cgcggctcgc
     1381 gatcgcccac gggcagatgc gcgagcgcga gctcgagcgc gtgatgcgcg atttcaccgc
     1441 gcagcgctgc aacgtgctgc tgtgctcgac catcatagag accggcatcg acattccgac
     1501 cgccaacacc atcgtcatcg accgcgccga caagttcggc ctggcgcagc tgcaccagct
     1561 gcgcggccgc gtcggccgct cgcaccacca ggcctacgcg tacttcctta ccccgccgca
     1621 cgatgcgctg aacaagaacg cgatcaagcg gctggaggcg atccagatga tggaggagct
     1681 cggctcgggc ttctacctgg cgatgcacga cctcgagatc cgcggcgccg gcgaggtgct
     1741 gggcgagtcg cagtcgggcg agatccagga gatcggcttc tcgctctacg cccggatgct
     1801 cgaccgcgcc gtgcggcgcc tcaaatcggg caaggacatc gattgggagg gcagcgtcga
     1861 cgtgagcacg gaagtgaatc tgcacgtgcc cgcgctcctc ccggcaagct actgcagcga
     1921 cgtgcacgag cggctctcgc tgtacaagcg cctggccgac gccgactcgc gcgaggcgct
     1981 cgacctgctg cgcgaggagc tggtcgaccg cttcggcgac ctgcccgagc cggcgcgcgc
     2041 gctgctcgag tgccaccagg tgcgcatcgc ggcgcgagcg ctcggcgtcg cgcgcgtcga
     2101 cgccacgcac gaggcggtgc agctgcagtt cgtcaagaat ccgccgctcg acggcgccaa
     2161 ggtcatcgcc ttcatcaagc ggcgcagcgg ggcgcgcctc accggccagg accgcctgcg
     2221 cgtcgagatg aagctgccgg cctggcagga gcgcggccag gcggtgcagg acatcctcaa
     2281 gcagctcgcc gcctgaaatc ggggtcagag cccgaattca atgaacctca tcctgcaaag
     2341 cccgaccctc gagcgcggcg acgtcgacgc gatcgccgac ggcgtgcggc catcgtccat
     2401 ggaaaagctg tcagccaggg cgttcagatt gaaaggcgcg acgccgcacg actcggtagc
     2461 gaagctcgcg gaggcgcggc gcatcgacta tgcctggatg gagcccggcc ggcgcttcgc
     2521 cgacctcaag ctcctcgcca tggacatgga ctcgaccctg atcaccatcg agtgcatcga
     2581 cgagctcggc gccatcgccg gcaagggcgc ggagatcgcg gcgatcaccg aagcggcgat
     2641 gcgcggcgag atcgccgact acaaggacag cctgcggcgg cgcgtgtcgc tcctggccgg
     2701 gcaaccagcc gggatcctgg agagcgtcta tcaaagcaag ctgaggttga cgccaggagc
     2761 cgagcagttg ctggacgctt gcaagcggca tggcgtgaag accctgctcg tttcgggcgg
     2821 cttcacgttc ttcaccggac ggctgaagga ccgcctgcgg cttgactaca cgatctcgaa
     2881 tacgctgcag accaaagcgg gcagcctgac cggcgctttg ctcggcgaca tcgtcgacgc
     2941 ggacgcgaaa gcggccaagt tcgtcgccgt catgaaagag ctgaaggcag gcaagcagca
     3001 gaccgtggcg atcgg
//
LOCUS       DASYJA010000411         2768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_970298, whole genome shotgun sequence.
ACCESSION   DASYJA010000411 DASYJA010000000
VERSION     DASYJA010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970298"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1528)
                     /locus_tag="VGO02_09010"
     CDS             complement(26..1528)
                     /locus_tag="VGO02_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEV7477860.1"
                     /translation="MSRIAIGGFQHETNTFAPSKADYAAFEAGGGWPGAKYGEPIFGA
                     VEGANIPAAGAVQALRALNHTMVGTAWAAASPSAHVTADAFERILSGLVENLKNAGLV
                     NGVYLDLHGAMVSEEFEDGEGEILRRVRELVGPRVPIVASLDLHANMTRAMVETVDAM
                     VAYRTYPHVDMADTGARAARLLDQMLKSGARLKRGFHTLDYLTGISSQCSFIEPCKSI
                     YGELSNLENKNNCMLSFTPGFPMADFEECGMSVFGYGPDEKSTNKAVEQLRRIVADAE
                     KDFALELHTAGDAVRRAKKLGEPGKPVVLADTQDNPGAGGNGDTTGLLKALIQQDAQD
                     AVLGLLIDAPSAKRAHEAGKGANAVFTLGGLSKIPGDAPLGGEFTVEALADGRFTCTG
                     PMFKGFRMTLGNMALLRSRAAPGVRVALASRKVQAADQEMFRHLGVEPRTQRILALKS
                     SVHFRADFEPMAREVLVVKSPGPALADPAEFNWTRLRKDIRLRPLGPVRA"
     gene            1564..>2768
                     /locus_tag="VGO02_09015"
     CDS             1564..>2768
                     /locus_tag="VGO02_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7477861.1"
                     /translation="MNRRLIAVLSLILCVSSVDAQEKVLRVVPHSNLSILDPIWTTQF
                     MARNHGYMIYDTLFGTDEKNRVQPQMVDKWTVSPDQRLWTFTLRPGLEFHDGRPVSGE
                     DVTASIERWGKRDAMGQKLMTFVERMDSPAPNSFRIFLREACGIMLEALGKPSSNVPF
                     IMPKRVAETPADKQIEEHIGSGPYVFKRDDFKPGDKAVYMKNTKYVPRKEAPSGTAGG
                     KFVYVDRVEWNLALRDGQAQVNALAKGEVDVLERPAFESYEQLKKADGVVLVNKDPLG
                     FQYMGRFNHLHPPFNNAKVRQAAIAAMQQEPFLRAQVGIKEYYKTCASMYTCGTPYGT
                     AKGSEIQSKASMKMAQEMLKASGYDGTPVVILKPTDLAAIQKLPDVAAQLLRQAGFKV
                     DLQAMDWNTV"
BASE COUNT          501 a          925 c          881 g          461 t
ORIGIN      
        1 cgcgcctaga gcgagcgcgc gcaggctatg cacggaccgg tccgagcggg cgcaggcgga
       61 tgtccttgcg cagcctggtc cagttgaact cggccgggtc ggcgagcgcg gggcccggcg
      121 atttcaccac cagcacttcg cgcgccatcg gctcgaagtc ggcgcggaaa tgcaccgagc
      181 tcttcaacgc caggatgcgc tgggtgcgcg gctcgacgcc gaggtggcgg aacatctcct
      241 gatcggcggc ctgcaccttg cgcgaggcga gggccacgcg taccccgggc gccgcccggc
      301 tgcgcagcag cgccatgttg ccaagcgtca tgcgaaagcc cttgaacatc ggcccggtgc
      361 aggtgaagcg gccgtcggcc agcgcttcga ccgtgaactc cccgccaagc ggcgcgtcgc
      421 ccggaatctt cgacaggccg ccgagagtga ataccgcgtt cgcccccttc cctgcttcgt
      481 gcgcgcgttt cgcagagggc gcatcgataa gcaatcccag tacggcgtcc tgggcatctt
      541 gctggattaa cgccttcaac aagcccgttg tgtcgccgtt gccaccagcg ccggggttgt
      601 cctgcgtgtc cgccagcacc accggcttgc ccggctcgcc gagcttcttc gcgcggcgca
      661 ccgcgtcgcc ggccgtgtgc agctcgagcg cgaaatcctt ctctgcgtcc gcgacgatcc
      721 gtcgcagttg ctctacagcc ttgttcgtgc ttttttcgtc cgggccatag ccaaacaccg
      781 acatgccgca ctcttcgaag tcggccatgg ggaaacccgg cgtgaacgac agcatgcaat
      841 tgtttttatt ttctaaatta ctcaattcgc cataaatgct cttgcagggc tcgatgaagg
      901 agcactgcga ggagatgccg gtgagatagt cgagcgtatg gaagccacgc ttgaggcgcg
      961 cgccgctctt cagcatctgg tcgagcaggc gagcggcgcg cgcgccggtg tcggccatgt
     1021 cgacgtgcgg gtaggtgcgg taggccacca tcgcgtcgac ggtttcgacc atcgcccgcg
     1081 tcatgttggc gtgcaggtcc aggctggcga cgatcggcac gcgcggcccg accagctcgc
     1141 gcacgcggcg caggatctcg ccttcgccgt cctcgaattc ctcggaaacc atcgcgccgt
     1201 gcaggtcgag gtacacgccg ttgaccaacc ctgcgttttt tagattttca accaaaccac
     1261 tcaaaattct ttcaaaagca tcggccgtca cgtgcgcgga cgggctcgcc gcggcccagg
     1321 cggtgccgac catggtgtgg ttcagtgcgc gcagcgcctg gactgcacca gcggcgggaa
     1381 tattggcgcc ctcgaccgca ccgaaaatgg gctcgccgta cttcgccccg ggccagccgc
     1441 cgcccgcctc gaacgccgca tagtccgcct tggacggggc gaaggtgttg gtctcgtgct
     1501 ggaagccgcc gatggcgatt cgactcatgg taggctggaa ttgtctctca ggaaggacgc
     1561 caaatgaacc gcagactgat cgctgttctt tcattgatcc tgtgcgtttc cagtgtagac
     1621 gcacaggaga aagtgctgcg cgtcgttccg cactccaacc tgagcatcct cgacccgatc
     1681 tggaccacgc agttcatggc gcgcaaccac ggctacatga tctacgacac cctgttcggc
     1741 accgacgaga agaaccgcgt gcagccgcag atggtggaca agtggacggt cagccccgac
     1801 cagcgcctgt ggaccttcac gctgcgcccg ggcctcgagt tccacgacgg cagaccggtc
     1861 agcggcgagg acgtcaccgc ctcgatcgag cgctggggca agcgcgacgc catggggcag
     1921 aagctgatga ctttcgtcga gcgcatggac tcgcccgcgc cgaacagctt ccgcatcttc
     1981 ctgcgcgagg cctgcggcat catgctcgag gcgctgggca agccctcctc caacgtgccg
     2041 ttcatcatgc ccaagcgcgt cgccgagacg ccggccgaca agcagatcga ggagcacatc
     2101 ggctcgggcc cgtacgtctt caagcgcgac gacttcaagc cgggcgacaa agcggtctac
     2161 atgaagaaca ccaagtacgt gccgcgcaag gaagcgccct cgggcaccgc cggcggcaag
     2221 ttcgtgtatg tcgaccgcgt cgaatggaat ctcgcgctgc gcgacggcca ggcgcaggtg
     2281 aacgcgctcg ccaagggcga ggtcgacgtg ctcgagcgcc cggcgttcga gagctatgag
     2341 cagctgaaga aggccgacgg cgtggtgctg gtcaacaagg acccgctcgg cttccagtac
     2401 atggggcgct tcaatcacct gcacccgccg ttcaacaacg ccaaggtgcg ccaggcggca
     2461 atcgccgcga tgcagcagga gccgttcctg cgcgcgcagg tcggcatcaa ggagtactac
     2521 aagacctgcg cttccatgta cacctgcggc acgccctacg gcaccgccaa gggcagcgag
     2581 atccagtcca aggccagcat gaagatggcg caggagatgc tgaaagcctc cggctacgac
     2641 ggcacgccgg tggtgatcct caagccgacc gacctcgccg cgatccagaa gctgcccgac
     2701 gtcgcggcgc agctcctgcg ccaggcgggc ttcaaggtcg acctgcaggc gatggactgg
     2761 aacacggt
//
LOCUS       DASYJA010000412         2376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2149685, whole genome shotgun sequence.
ACCESSION   DASYJA010000412 DASYJA010000000
VERSION     DASYJA010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2149685"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="VGO02_09020"
     CDS             complement(<1..1096)
                     /locus_tag="VGO02_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEV7477862.1"
                     /translation="MPKRLGFTCGPDELRGLAQAVLERAKLGGASGCECDVSEGYGLS
                     VTVRKGQADVVEHNRDRSIGVSVYLGSRPKARRGHASTSDFSRAALEQTVDAALAIAR
                     HTAEDDCAGLPEPEQLARSQPDLDLYHPWSIDTEEAIELARRCEAAAFGVSKKISNSE
                     GATVSAQQSQFVFANSLGFSGGHAGSRHYLACSVIAENKGLMQRDDWHSASRVPERIA
                     EPRALGRYAAERALARLGGRKIPTRQAPVLFEAPVAIGLIGHFISAVNGGNLYRKTSF
                     LADSLGQQVFSPRVGIEERPFERQGLASSAFDEEGVATRKRAIVRDGVVEGYFLGSYS
                     ARKLGMQSTGSAGGHHNLVVSHGDLDLRGML"
     gene            1118..1615
                     /gene="yjgA"
                     /locus_tag="VGO02_09025"
     CDS             1118..1615
                     /gene="yjgA"
                     /locus_tag="VGO02_09025"
                     /inference="COORDINATES: protein motif:HMM:NF016626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HEV7477863.1"
                     /translation="MQDEIVSKTKRKREMHDLQALGVALVALPESQLDGLSLDDNLHK
                     AVLEAKRISAHEGRRRQMQYIGRLMRDVDPAPIRAKLEEISGGSAQANARHKRLESLR
                     ERLLADDDALTQFAAAHPSADLQALRALIRNSRKEQKEGKPPRAYRELFRLLKAIESS
                     TPLSS"
     gene            1587..2036
                     /locus_tag="VGO02_09030"
     CDS             1587..2036
                     /locus_tag="VGO02_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HEV7477864.1"
                     /translation="MNLQPLFRAEITLAAAQELGETPHGRRRIIPITGGRIAGERLSG
                     RVLPGGADWQVVRADGVAELDARYTIETADGALVYVANRGVRHGPPEVIRKLAAGEPV
                     DPALYYMRTTPWFETGDARYAWLNRIVCVATGARRAAAVELEVFEIK"
     gene            2033..>2376
                     /gene="mog"
                     /locus_tag="VGO02_09035"
     CDS             2033..>2376
                     /gene="mog"
                     /locus_tag="VGO02_09035"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HEV7477865.1"
                     /translation="MTELVVGLVSISDRASAGVYKDEGIPALKEWLNAAIASPAWRDE
                     TRLIADEQPTIEGTLKELVDRQGCHLVLTTGGTGPAPRDVTPEATLAVAEKVMPGFGE
                     QMRAVSLKYVPTA"
BASE COUNT          372 a          835 c          832 g          337 t
ORIGIN      
        1 gcagcatgcc gcgcaagtcg agatcgccgt ggcttaccac caggttgtgg tggccgccgg
       61 cgctgccggt gctttgcatg ccgagcttgc gcgcggaata gctgcccagg aaatagccct
      121 cgaccacgcc gtcgcgcacg atggcgcgct tgcgcgtggc gacgccctcc tcgtcgaacg
      181 cgctgctcgc caacccctgg cgctcgaaag gccgctcctc gatgcccacc cgcggcgaaa
      241 acacctgctg gcccaggctg tccgcgagga acgaggtctt gcggtagagg ttgccgccgt
      301 tcacggcgga gatgaagtgc ccgatcaggc cgatcgccac cggcgcctcg aacagcaccg
      361 gcgcctggcg ggtgggaatc ttgcggccgc ccaggcgcgc cagcgcgcgc tcggcggcat
      421 agcggccaag ggcgcgcggc tcggcgatgc gctccggcac gcgcgaggcg ctgtgccagt
      481 cgtcgcgctg catgagcccc ttgttctcgg cgatgaccga gcaggctagg tagtggcgcg
      541 agccggcgtg cccgccgctg aaccccaggc tgttggcgaa gacgaactgc gattgctgcg
      601 cggagacggt ggcgccctcg gagttgctga tctttttcga cacgccgaag gcggccgcct
      661 cgcagcgccg ggcgagctcg atcgcctcct cggtgtcgat gctccaggga tggtagagat
      721 cgaggtcggg ctggctgcgc gccagctgct ccggctcggg cagcccggcg cagtcgtcct
      781 ccgcggtatg gcgcgcgatg gcaagcgcgg cgtccaccgt ctgctcgagc gccgcgcgcg
      841 agaaatccga tgtgctggca tgcccgcggc gcgccttcgg ccggctgccg aggtacaccg
      901 agacgccgat cgagcggtcg cggttgtgct cgacgacgtc ggcttggccc ttgcgcaccg
      961 tcacgctcag gccgtagccc tcgctcacgt cgcactcgca gccgctcgcg ccgccgagct
     1021 tcgcacgctc gagcaccgcc tgcgcgaggc cgcgcaattc gtccggcccg caggtgaatc
     1081 cgaggcgctt cggcatgccg cgctatgata gcgcgccatg caggacgaaa tcgtcagcaa
     1141 gacgaaacgc aagcgcgaga tgcacgacct gcaggcgctc ggcgtggcgc tggtggcgct
     1201 gccggagtcg cagctcgacg gcctttcgct cgacgacaat ctccacaagg cggtgctcga
     1261 ggcaaagcgt atctccgccc acgagggccg gcgccggcag atgcagtaca tcggcaggct
     1321 gatgcgcgac gtcgacccgg cgccgatccg cgccaagctc gaggagatct cgggcggctc
     1381 ggcgcaggcg aacgcccggc acaagcgcct cgagtcgctg cgcgagcggc tgctggccga
     1441 cgatgacgcg ctcacgcagt tcgccgcggc gcatccatcg gcagaccttc aggcgctgcg
     1501 ggccctgatc aggaattcga ggaaggaaca aaaggaaggc aagccgccgc gggcctaccg
     1561 cgaattgttc aggctgttga aggcgattga atcttcaacc cctctttcga gctgagatca
     1621 cgctcgcggc tgcgcaggag ctgggcgaga ccccgcacgg gcggcgccgc atcatcccga
     1681 tcaccggcgg gcgcatcgcc ggcgagcgcc tgtcgggccg cgtgctgccg ggcggggccg
     1741 actggcaggt ggtgcgcgcc gacggcgtcg ccgagctcga cgcgcgctac accatcgaga
     1801 ccgccgacgg cgccctcgtc tacgtcgcca accgcggcgt gcgccacggc ccgcccgaag
     1861 tcatccgcaa gctggccgcc ggcgagcccg tcgaccccgc gctttactac atgcgcacca
     1921 ccccttggtt cgagaccggg gacgcgcgct acgcctggct gaaccgcatc gtgtgcgtcg
     1981 ccaccggcgc gcgccgcgcc gccgcggtcg aactcgaggt gtttgaaatc aaatgactga
     2041 actggtagtg ggtctggtgt ccatcagcga tcgcgcctct gccggcgtgt acaaggacga
     2101 aggcatcccg gcgctcaagg aatggctcaa cgccgcaatc gcctcgccgg cctggcgcga
     2161 cgagacgcgc ctgatcgccg acgagcagcc gacgatcgag ggcacgctca aggagctagt
     2221 cgaccgccag ggctgccacc tggtgctcac gaccggcggc acgggaccgg cgccgcgcga
     2281 cgtcacgccc gaggcgactc tcgccgtcgc ggaaaaggtg atgccggggt tcggggagca
     2341 gatgcgggca gtgagcctga agtatgtgcc cacagc
//
LOCUS       DASYJA010000413         1706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1878969, whole genome shotgun sequence.
ACCESSION   DASYJA010000413 DASYJA010000000
VERSION     DASYJA010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1878969"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VGO02_09040"
     CDS             complement(<1..599)
                     /locus_tag="VGO02_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV7477866.1"
                     /translation="MRAAKSFIGLWLAVLSLSAFAQARELPDFTRLVEEQGGSVVNIS
                     TTQAARRSALPQVPGMEDDEVMEFFRRFVPRQPPGQGPERGPGRPESRSLGSGFILSA
                     DGYILTNAHVVDSADEINVKLTDKREFKAKVIGADKRTDVALIKIEGTSLPVVKFGDP
                     NKLKVGEWVVAIGAPFGFENTVTAGIVSAKGRSLPQENFV"
     gene            complement(633..1598)
                     /locus_tag="VGO02_09045"
     CDS             complement(633..1598)
                     /locus_tag="VGO02_09045"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HEV7477867.1"
                     /translation="MTLCRALGFSALLCAGLAQAQTPEALDWLRRIHDATHRLSYSGT
                     FVYQNGNRSETSRITRYVDAGGDIEKVEVMDGVPREIVRTRGTVRCYLPDSRVVKVEQ
                     QIDRAGAERGFPAFLPERINALARHYDIAMGDTQRVAGYDCRTVTLIPKDDLRYGYML
                     YADVKSGMLLKAETFDAKGALVEQFSFTQLAIGNVSRDKVKTRHAAQSWRVENSEAAP
                     ASLAGWGLSSELPGFRKVAELRRRVGEAGAVGQVVYSDGLAAVSVFIEPLEGRRDPVR
                     TGLASVGAIHIYTREVAKHMVTVVGEAPAASVQRIANAVEFRRPQ"
BASE COUNT          240 a          598 c          573 g          295 t
ORIGIN      
        1 acgaagttct cctgcggcag cgagcgcccc ttggcgctga cgatgccggc ggtgaccgtg
       61 ttctcgaagc cgaacggggc gccgatcgcc accacccatt cgccgacctt gagcttgttc
      121 gggtcgccga acttgaccac cggcaggctg gtgccctcga tcttgatcag cgccacgtcg
      181 gtgcgcttgt cggcgccgat caccttggcc ttgaactcgc gcttgtcggt cagcttgacg
      241 ttgatctcgt cggcgctgtc gacgacgtgg gcgttggtga ggatgtagcc gtcggcgctc
      301 aggatgaagc ccgagccgag cgagcgactc tcggggcgtc cgggtccccg ctcggggccc
      361 tgcccggggg gctggcgcgg cacgaagcgg cggaagaact ccatcacctc gtcgtcctcc
      421 atgccgggca cctgcggcag cgcgctgcgc cgcgccgcct gggtggtgct gatgttgacg
      481 accgaaccgc cctgctcttc caccagccgg gtgaagtcgg gtagctcgcg cgcctgcgcg
      541 aacgcgctca ggctgaggac agccagccac aggccaataa aggattttgc tgctcgcatc
      601 ggtttctcca gtttcttgat ggtactgctt tgctactgcg ggcggcggaa ctccacggca
      661 tttgcgatgc gctgcacgct cgccgcgggt gcctcgccga ccacggtaac catgtgcttc
      721 gccacttccc tggtgtagat atggatcgcg ccgacgctgg ccaggccggt gcgaaccggg
      781 tcgcggcgcc cctcgagcgg ctcgatgaac accgagaccg ccgccaggcc gtccgagtag
      841 accacctgcc cgaccgcccc tgcctcgccc acgcggcgcc tcagctccgc caccttcctg
      901 aatcccggca gctcgctcga caggccccag cccgcgaggc tcgccggcgc ggcttcggaa
      961 ttctccaccc gccagctctg cgcggcgtgg cgcgtcttta ccttgtcgcg cgagacgttg
     1021 ccgatggcga gctgggtgaa gctgaactgc tcgaccagag cgcccttggc gtcgaacgtc
     1081 tcggccttga gcagcatgcc gctcttcacg tccgcgtaca gcatgtagcc gtagcgcaga
     1141 tcgtccttgg ggatcagcgt caccgtgcgg cagtcgtagc ccgccacgcg ctgggtgtcg
     1201 cccatcgcga tgtcgtagtg gcgcgcgagc gcgttgatgc gctccggaag aaacgccggg
     1261 aagccgcgct ccgccccggc gcggtcgatt tgctgctcga ccttcaccac ccggctgtcg
     1321 ggcaggtagc agcgcacggt gccgcgcgtg cgcacgatct cgcgcggcac gccgtccatc
     1381 acctcgacct tctcgatgtc gccgccggcg tcgacgtagc gcgtgatgcg cgaggtctcg
     1441 ctgcggttgc cgttctggta gacgaaggtg ccgctgtagg agagcctgtg cgtcgcatcg
     1501 tggatcctgc gcagccagtc gagggcttcg ggcgtctgcg cctgggcgag cccggcgcac
     1561 agcagcgcgg agaagcccag cgcccggcaa agggtcactt gcgcggcccc cggccctgct
     1621 cggaaacgct gcgcacgtaa ggcgccatgc cctgcaggga gacgcgcggc gaaaacccct
     1681 ggtgcgcgag cagatagtcg ttggtc
//
LOCUS       DASYJA010000414         3005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_61945, whole genome shotgun sequence.
ACCESSION   DASYJA010000414 DASYJA010000000
VERSION     DASYJA010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61945"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VGO02_09050"
     CDS             <1..778
                     /locus_tag="VGO02_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEV7477868.1"
                     /translation="GEIDAYTEFVKALGAKGLAYIKVNEKAKGAEGLQSPIVKNLHLA
                     AIKAILERSGAQDGDLIFFGADREAACNAYMGALRQKVGHDRGLAQKGWRPLWVVDFP
                     MFEYDEETKEWRARHHPFTSPKDGHEAYIGAAPEKAYAKAYDLVLNGWEIAGGSVRIH
                     RQEVQQKVFEALKISPEQQRAKFGFLLDALQYGAPPHGGIAFGFDRLVTLMAGAEAIR
                     DVIAFPKTQRGQDLLTGAPTPVSEKQLRELHLRLRNPPQS"
     gene            775..1101
                     /locus_tag="VGO02_09055"
     CDS             775..1101
                     /locus_tag="VGO02_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HEV7477869.1"
                     /translation="MRLLAALLALAFSFAVLGKDVRSGELPKEARQTLELIRSNGPFP
                     YRQDGATFGNREKRLPLRANGYYREYTVKTPGAKDRGPRRIIAGNGGEFYYTEDHYRS
                     FMRVRE"
     gene            1101..1505
                     /locus_tag="VGO02_09060"
     CDS             1101..1505
                     /locus_tag="VGO02_09060"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEV7477870.1"
                     /translation="MGKLLQRLQDASRSGVYRVRTDAEVLDALRGSGLKKISLQGITT
                     KSGLLERIARTLEFPEWFGGNWDALEDCLSEVRGYLLVYDYQAIPADDLGVLLDVLAS
                     SAEFHAEQGEPFFAIFVDPQNALDLDDLFREA"
     gene            1502..1942
                     /gene="nudB"
                     /locus_tag="VGO02_09065"
     CDS             1502..1942
                     /gene="nudB"
                     /locus_tag="VGO02_09065"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEV7477871.1"
                     /translation="MSYKLPVSVLVVVHSAALEVLLLERASHPGYWQSVTGSLDRLDE
                     PLEQAAAREVREETGIEIARGSLVRWNVTNNFEIYATWRHRFAPGVTHNTEHVFGLTL
                     PGRMPVTLSAEEHTAFQWLPWREAAEKCFSWSNRDAIRMLGERA"
     gene            1939..2685
                     /locus_tag="VGO02_09070"
     CDS             1939..2685
                     /locus_tag="VGO02_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003269634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV7477872.1"
                     /translation="MSQLRVVTLNIHKGLSQFNRRMVLHDLREGLRALSPDVMFLQEV
                     QGLNQRFALRYAVSPPGAQHEFLAGDHWQHVYGCNAVYDHGHHGNAILSRFPFVSSEN
                     TDVSDHRFERRGLLHSVVAVPGWRRNLHLVCVHLSLHERGRSRQIEAISGRLEELASR
                     DVPIIVAGDFNDWRHRASAILERRLGMTEVSVAHHGRALATYPSLLPFLRLDRIYVRG
                     FQVTASKVHRGKPWSMLSDHLAVSAELERG"
     gene            complement(2695..>3005)
                     /locus_tag="VGO02_09075"
     CDS             complement(2695..>3005)
                     /locus_tag="VGO02_09075"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEV7477873.1"
                     /translation="RETVLIVCSGSGNNFNFEDFYGAGYFVECFAARLGAAADFSDSA
                     KAARALYHQARAPEALLDCRIGRLMASRGLAHEVEFACRLDAFPVVPALQGGSLRLVD
                     "
BASE COUNT          519 a         1000 c         1016 g          470 t
ORIGIN      
        1 cggcgagatc gatgcctaca ccgagttcgt caaggcgctc ggcgccaagg ggctcgccta
       61 catcaaggtg aacgagaagg cgaagggcgc cgaaggcctg cagtcgccga tcgtgaagaa
      121 cctgcatcta gctgcgatca aggcgattct cgagcgctcc ggcgcccagg acggcgacct
      181 gattttcttc ggcgcggacc gggaagccgc gtgcaacgcc tatatggggg cgctgcggca
      241 gaaggtgggc catgaccgcg ggctcgcgca gaagggctgg cggccgctgt gggtggtcga
      301 tttcccgatg ttcgagtacg acgaggaaac gaaggagtgg agggcgcgcc accacccgtt
      361 caccagcccg aaggatgggc acgaggcgta catcggggcc gcaccggaga aggcgtacgc
      421 gaaggcctac gacctggtgc tgaacggctg ggagatcgcc ggcggctcgg tgcgcatcca
      481 ccgccaggaa gtgcagcaga aggtgttcga ggcgctcaag atcagccccg agcagcagcg
      541 cgccaagttc ggcttcctgc tcgacgcgct gcaatacggc gctccgcccc atggcggcat
      601 cgccttcggc tttgaccgtc tggtgacgct gatggccggc gcggaggcca tccgtgacgt
      661 catcgccttc ccgaagacgc agcgcggcca ggacctgctg accggcgcgc ccaccccggt
      721 gagcgaaaag cagctgcgcg agctgcacct tcgcctgagg aacccgccgc agtcgtgaga
      781 ctgctcgccg cgctcctggc gctcgctttc tcgtttgccg tcctcggcaa ggacgtccgt
      841 tccggcgagc tgccgaaaga agcgaggcag accctcgagc tgatcaggag caacggtccg
      901 tttccgtacc gccaggacgg cgccaccttc ggcaaccgcg agaagcgcct gccgctgcgc
      961 gccaacggtt actaccgcga gtacacggtg aagacgccgg gagcgaagga tcgcggtccg
     1021 cgccgcataa tcgctggtaa cggcggcgag ttctactaca cggaagacca ctaccgatcg
     1081 ttcatgagag tgcgcgaata atgggcaagt tgctgcaacg cctgcaggac gcttcgcgct
     1141 cgggggtcta ccgcgtgcgc accgacgccg aagtgctgga cgctttgcgc ggaagcgggc
     1201 tgaaaaaaat ctctctccag ggaatcacga cgaaaagcgg tctcctggag cggattgccc
     1261 gcacgctcga gttccccgag tggttcggcg gcaactggga cgcgctggag gattgtctct
     1321 cggaggtgcg cggctatctg ctcgtctatg actaccaggc aatcccggcg gacgatctcg
     1381 gcgtgctgct cgacgtgctt gcatcgagcg ccgagttcca cgcggagcag ggcgagccgt
     1441 tcttcgcgat tttcgtcgac ccgcagaacg ccctcgacct ggacgacctg ttccgcgagg
     1501 catgagctac aagctgccgg tgtcggtgct ggtagtggtg cacagcgcgg ccctcgaggt
     1561 gctcctcctc gagcgcgcct ctcatccggg ctattggcag tcggtcaccg gctcgctcga
     1621 ccggctcgac gagcccctcg agcaggcggc cgcccgggaa gtgcgcgagg aaacgggcat
     1681 agagattgcg cggggtagcc tggtgcgctg gaatgtcacg aacaatttcg agatctatgc
     1741 cacgtggcgc caccgcttcg cgccgggcgt cacgcacaat accgaacatg ttttcggact
     1801 cacgctgcca gggcgcatgc cggtgaccct ctctgccgag gagcacacgg ctttccagtg
     1861 gctgccgtgg cgcgaggcgg ccgagaagtg cttttcctgg tcgaaccgcg atgcgatccg
     1921 catgctgggc gagagagcat gagccagctg cgcgtcgtca cgctcaacat ccacaaaggc
     1981 ctgtcgcagt tcaaccggcg catggtgctg cacgacctgc gcgaagggct gcgcgcgctc
     2041 tcgcctgacg tcatgttcct gcaggaggta caggggctga accagcggtt cgccctgcgc
     2101 tacgccgtat ctccgccggg agcgcagcac gagttcctgg cgggcgacca ctggcagcac
     2161 gtctacggtt gcaacgcggt ctacgaccat ggccaccacg gcaacgctat cctgtcgcgc
     2221 ttcccgttcg tctcgtccga gaacaccgac gtctccgacc accgcttcga gcgccgcggg
     2281 ctgctgcaca gcgtggtggc ggtgcccggc tggcggcgca acctgcacct ggtatgcgtc
     2341 catctctcgc tgcacgagcg cggccgaagc cgacagatcg aggcgatctc ggggcggctg
     2401 gaggagctcg cctcgcgcga cgtgccgatc atcgtggcgg gcgacttcaa cgactggcgc
     2461 caccgcgcca gcgccatcct cgagcggcgc ctgggcatga ccgaggtgtc ggtggcgcac
     2521 cacggccgcg ccctcgccac ctacccgagc ctgctgccgt tcctcaggct cgaccgcatc
     2581 tacgtgcgcg gcttccaggt caccgcctcg aaagtgcacc gcggcaagcc ctggtcgatg
     2641 ctctcggacc acctcgcggt cagcgccgag ctcgagcgcg gctagagcgc gccgtcagtc
     2701 gaccagccgc aggctgccgc cttgcagcgc cggcaccacc gggaaggcgt cgagccggca
     2761 ggcgaattcc acttcgtggg cgaggccgcg cgaggccatc aggcggccga tgcggcagtc
     2821 gagcagggcc tcaggcgcgc gggcttggtg gtacaaggcc cgcgcagcct tggctgagtc
     2881 ggagaaatcc gccgccgcgc ccaggcgagc ggcgaagcac tcgacgaaat agccggcgcc
     2941 gtagaaatcc tcgaagttga agttgttgcc cgacccggag cagacgatga gcacggtctc
     3001 ccgcg
//
LOCUS       DASYJA010000415         7073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_971607, whole genome shotgun sequence.
ACCESSION   DASYJA010000415 DASYJA010000000
VERSION     DASYJA010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971607"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VGO02_09080"
     CDS             <1..289
                     /locus_tag="VGO02_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7477874.1"
                     /translation="QALQRTANFAISNPAREVLFTVLARDEKYKAKNVIDIVAVRGAD
                     AAGGWFFTGLRSLGMEVRSIPLVAILLAAVCLALATALGRAQERRAAAPRL"
     gene            324..911
                     /locus_tag="VGO02_09085"
     CDS             324..911
                     /locus_tag="VGO02_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV7477875.1"
                     /translation="MGFWIFLGIIVALVVWAISIYNGLVTLRNRFKNAFAQIDVQLKR
                     RYDLIPNLVEIAKGYIKHERETLEAVIKARNSAQSAAQAAAAAPGNPAAMQQLGAAEG
                     ALTGVLGRMFALAEAYPDLKANQNMLSLQEELSSTENKVAFARQAFNDSVMEYNTKRE
                     SFPDSIFAGMFGFGPAELLAATESAEERKAPKVSF"
     gene            918..2852
                     /locus_tag="VGO02_09090"
     CDS             918..2852
                     /locus_tag="VGO02_09090"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEV7477876.1"
                     /translation="MNFFEHQALARRNSRMMVVLFLLAVVAVVSAVDCVIAAVYLGAA
                     DAPVHQVPASVYVWGALITAGTIFVVSLIHVTRLGGGGAVVAAMAGARRVASNSTDPL
                     ERRLVNIVEEMAIASGVRVPQVYIMDKESGINAFAAGWEVSNAVVAVTRGALDTLNRD
                     ELQGVVGHEFSHILNGDMRLNIRMLGVLAGIVFIGSVGAFGMRSAGEADDIRAAAVVF
                     VIGLALFIVGYVGLFFGHLIKAAIARQREYLADASSVQFTRNPDGIAGALDQIRGAQK
                     GALISARCAEDLSHMFFGESLRQRLGGLLATHPSLDARIKRVNPRFQPSTYRQRRPAQ
                     AAEPAPAEAAGFAGEAAPAGRRAGDAGAAWGRSPAESANLVGSVDAGKLDHAARLLAS
                     LPAPLREALREPDGASAAMVALLIAPKEDVMRQQLDAVARATTLAERVRGLLAHTSGL
                     GLGFHLPVIDLALATIKPAPESTKRNVVAALEAAAYADRRVSLHEFVVLTLVRDQLAP
                     RTKPGAVPSKRIAQLEGAAGTVLALMAHAGTRGDATGARQDALQAALRAGATIMGIKE
                     AAAANAALNLDAARNALEALKELAPMQKAVLVKGLFAAVTADGTIRVAEAGLMRLVGA
                     VLDCPLPPLLAEVDPASLQE"
     gene            complement(2943..3950)
                     /locus_tag="VGO02_09095"
     CDS             complement(2943..3950)
                     /locus_tag="VGO02_09095"
                     /inference="COORDINATES: protein motif:HMM:NF033920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA2778 family cysteine peptidase"
                     /protein_id="HEV7477877.1"
                     /translation="MVVLLQEVTALGRKAVENARVAAGVFTFIAFAALQGCANLVPQT
                     SALLEGLPGDLPPRVELTGVPFFPQSEYQCGPAALATVLANAGVATTPEALVPQVYLP
                     ARKGSLQVEMLAAARRNGLVSYQLAPSFTDLMRELNAGNPVIVLQNHGAIFDGWHYAV
                     AVGYDYKLGMMILRSGITEREEMPFGVSEFVWKRASYWAMVAVPPERIPVTAEEGRWL
                     GSLVAFERLNDPQKNRAAYSAFLQRWPGNVNAAVGLANAHHALGELPQAEKVLRAAMT
                     REPDSAIVLNNLAQTLSDLGRDAEALPIVERAVAAGGPHAAAVRQTRDEILKKIETKK
                     N"
     gene            complement(3926..4291)
                     /locus_tag="VGO02_09100"
     CDS             complement(3926..4291)
                     /locus_tag="VGO02_09100"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HEV7477878.1"
                     /translation="MKSVWTRTICRVLVALMIWTPYYIAQAGVITTDQAVGSAASQAD
                     RTTVLNFLTRGDVTSQLQSLGVDPSSAKQRVAALSDAEAASLAQRINAMPAGASDDWA
                     WVLLVIVIAAGVWWFFFRK"
     gene            4440..>7073
                     /locus_tag="VGO02_09105"
     CDS             4440..>7073
                     /locus_tag="VGO02_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HEV7477879.1"
                     /translation="MVEIRRLEAALRTAVAGEVRFDAGARAAYSADASNYRQVPIGVV
                     LPRSAADIEAAVAVCREHGAPILPRGCGTSLNGQTVNVAVVIDCSKYLDRIVALDPAA
                     RLARVEPGVVCDSLREAAEAHGLTFGPDPATHSRCTLGGMIGNNSCGAHSVMAGKTAE
                     NIARLEVLTYSGCRFWCGPTNSAEFDSIQRKGGEQAEIYLRLKRLIDKYGDLVRKQFP
                     KIRRRVSGYNLDQLLPENGFNVARALVGTEGGCAITLQAEARLVTSPPSRVLLVMAFA
                     DIYAAGDAVPQVLAAGPIACEGLDEAIIGGLRSRGLRLEDIALLPPGKAWLMVEFGAD
                     TQPEAIEKAKQLDGNLIVKKSLISRLWTIRETGASATALNLRGEGADPVVGWEDAAVD
                     PMRLGDYLREFQALIDRMGYRTSLYGHFGDGCIHARIDFRLRTPEGLAQWRRFLTEAA
                     HLVVKYGGSLSGEHGDGQAKAEFLPIMYGPELMQAFREFKAIWDPRHRMNPNKLIDPR
                     RADEDLRLGPQYKPVTLNTKFAFTTDVGTGFTRATEHCIGMGKCRSASGGTMCPSYRA
                     TREERYSTRGRGRLLAEMLRGEVITDGWRSEEVKEALDWCLACKGCRSDCPTHTDMAA
                     YKAEFLSHYYETKARPLVAHLMGRIGEWAPLAARASWAVNGLSAVLRPPGIARQRSLP
                     KFRKTFRSTFASGGQQGERVVLFDDTFNNHFRPQTARAAQKLLESAGCAVELPREHLC
                     CGRPYYDYGMLDRAKGALNRVLEVLEPGVPVVMLEPGCLSVFRDEMKQLFPRDERAAR
                     LEVFSLSEFLLKRNFKVKNAASVVVHSHCHQKAIWGEKSDIDLLRAAGCSVTAPDTGC
                     CGMAGSFGYKAQHYEASKLIFG"
BASE COUNT         1133 a         2395 c         2447 g         1098 t
ORIGIN      
        1 ccaggcgctg cagcgcacgg cgaacttcgc catctccaac cccgcgcgcg aggtgctgtt
       61 cacggtcctc gcgcgcgacg agaagtacaa ggcgaagaac gtgatcgaca tcgtcgcggt
      121 gcgcggcgcc gacgccgcgg gcggctggtt cttcaccggg ctgcgctccc tcggcatgga
      181 ggtccgctcg attccgctgg tcgcgatcct gctcgccgcc gtctgcctcg cgctcgccac
      241 ggcgctcggc cgggcgcagg agcgccgcgc ggcggcgcct aggctatagt aaggcgacga
      301 aggtaaaaat cagggggtag gacatgggct tctggatctt cctcggcatc atcgtcgcgc
      361 tggtcgtctg ggcgatctcg atctacaacg gcctggtgac gctgcgcaac cgcttcaaga
      421 acgcgttcgc gcagatcgac gtgcagctca agcggcgcta cgacctgatc cccaacctgg
      481 tcgagatcgc caagggctac atcaagcacg agcgcgagac gctcgaggcg gtgatcaagg
      541 cgcgaaattc tgcacaaagc gccgcccagg ccgcagccgc cgcgccgggc aacccggcag
      601 ccatgcagca gctgggcgcc gcggaaggcg cgctcaccgg cgtgctcggc cgcatgttcg
      661 ccctcgccga ggcgtacccg gacctcaagg cgaaccagaa catgctctcg ctgcaggagg
      721 agctctccag caccgagaac aaggtggcgt tcgcgcgcca ggcgttcaac gactcggtga
      781 tggaatacaa caccaagcgc gaatcgttcc ccgacagcat cttcgccggc atgttcggct
      841 tcggcccggc ggagctgctc gccgccaccg agtcggctga agagcgcaag gcacccaaag
      901 tctctttcta gcctggcttg aatttcttcg agcatcaggc gctcgcccgg cgcaacagcc
      961 ggatgatggt ggtgctgttc ctccttgccg tggtggcggt ggtgagcgcg gtcgattgcg
     1021 tgatcgcggc ggtctacctg ggcgccgccg atgccccggt acaccaggtg ccggcgagcg
     1081 tctacgtctg gggcgccttg atcacggccg ggactatctt cgtggtgagc ctgatccacg
     1141 tcacccggct cggcggcggc ggcgccgtgg tggcggcgat ggccggcgcg cggcgcgtgg
     1201 cgtcgaacag caccgacccg ctcgagcggc ggctggtgaa catcgtcgag gagatggcga
     1261 tcgcctcggg ggtgcgggtg ccgcaggtgt acatcatgga caaggagagc ggcatcaacg
     1321 ctttcgccgc cggctgggag gtatccaacg cggtggtggc ggtgacgcgc ggcgcgctcg
     1381 acacgctcaa ccgcgacgag ctgcagggcg tggtcggcca tgagttcagc cacatcctga
     1441 acggcgacat gcggctcaac atccgcatgc tcggcgtgct cgccggcatc gtgttcatcg
     1501 gctcggtcgg cgcgttcggc atgcgcagcg cgggcgaagc ggacgacatc cgcgccgcgg
     1561 ccgtggtctt cgtgatcggg ctggcgctgt tcatcgtcgg ctatgtcggc ctgttcttcg
     1621 gccacctcat caaggccgcg atagcgcgcc agcgcgagta cctggccgat gcctcgtcgg
     1681 tgcagttcac ccgcaacccg gacggcatcg cgggcgccct cgaccagatc cgcggcgcgc
     1741 agaagggggc gctgatctcg gcgcgctgcg ccgaggacct gtcgcacatg ttcttcggcg
     1801 agagcctgcg ccagcggctc ggcggactgc tcgccaccca tccttcgctc gacgcgcgca
     1861 tcaagcgcgt caacccgcgc ttccagcctt cgacctaccg ccagcggcgg ccggcccagg
     1921 cagcggagcc agcgccggct gaggctgccg ggttcgccgg cgaggccgcg cccgcggggc
     1981 gccgcgccgg cgatgcgggc gccgcctggg gccgctcgcc cgccgagagc gccaacctcg
     2041 tcggctcggt cgacgccggc aagctcgacc atgcggcgcg cctgctcgct agcctgccgg
     2101 cgccgctgcg cgaagcgctg cgcgagcccg acggcgcgag cgccgcgatg gtcgcgctgc
     2161 tcatcgcgcc caaggaggac gtcatgcgcc agcagctcga cgcggtcgcc cgcgcgacca
     2221 cgctggccga gcgcgtgcga gggctgctgg cgcacaccag cggcctcggc ctgggcttcc
     2281 acctgccggt gatcgatctc gcactggcga cgatcaagcc cgcgcccgag tccaccaaga
     2341 ggaacgtggt tgccgcgctc gaggccgcgg cctatgccga ccggcgcgtt tcgctgcacg
     2401 agttcgtggt cctaaccctg gtgcgcgacc agctcgcgcc gagaaccaag cctggcgccg
     2461 tgccctcgaa gcggatcgcg cagctcgaag gcgcagccgg cacggtgctc gcgctgatgg
     2521 cgcacgccgg gacgcgcggc gacgcgaccg gcgcgcgcca ggacgccttg caggcggcgc
     2581 tgcgcgccgg ggcgacgatt atgggcatca aggaagctgc ggcggcgaac gccgcgctca
     2641 acctcgacgc cgcgcgcaac gcgctcgaag cgctgaaaga actcgcgccg atgcagaagg
     2701 cggtcctggt gaagggcctg ttcgccgcgg tgacggccga cggcacgatc cgcgtcgccg
     2761 aagccggcct gatgcgcctg gtgggggcgg tgctcgattg cccgctgccg ccgctgctcg
     2821 ccgaggtcga ccccgccagc ctgcaggagt aggaattcgg ggtcagggac cgatctctgc
     2881 agaatcgccg acggcgattc tctggcggag atcggaccct gaccccgaat tcagctgcag
     2941 gcctagtttt tcttggtttc gatttttttc aggatctcgt cgcgcgtctg gcgcaccgcc
     3001 gcggcatgcg gcccgccggc ggcgacggcg cgctcaacga tcggcagcgc ctccgcgtcg
     3061 cgtcccaggt cggagagtgt ctgcgccagg ttgttgagca cgatcgccga atccggctcg
     3121 cgcgtcatgg cagcgcgcag caccttctcc gcctgcggca gctcgccgag cgcgtggtgc
     3181 gcattggcga ggccgacggc agcgttcaca ttgcccggcc agcgctgcag gaatgcggaa
     3241 taggcagcgc ggtttttttg cgggtcgttc aggcgttcga aagcgaccag cgagcccagc
     3301 cagcggccct cctcggcggt gaccggtatg cgctcgggcg gcaccgccac catcgcccag
     3361 tagctggcgc gcttccagac gaactcgctc accccgaacg gcatctcttc gcgctcggtg
     3421 atacccgagc gcaggatcat catgccgagc ttgtagtcgt agcccaccgc cacggcgtaa
     3481 tgccagccgt cgaagatggc gccgtggttc tgcagcacga tgaccgggtt gccggcattc
     3541 agctcgcgca tcaggtcggt gaagctcggg gcgagctggt aggagacgag gccgttgcgg
     3601 cgcgccgcgg cgagcatctc gacctgcagg ctgcccttgc gcgcgggcag gtagacctgc
     3661 ggcaccagcg cttccggcgt ggtggcgacg cccgcgttgg ccagcacggt ggcgagcgcc
     3721 gccggcccgc actggtactc gctctgcggg aagaacggca ccccggtcag ctcgacgcgc
     3781 ggcggcagat ccccgggcag cccctcgagg agcgccgagg tctgcggcac caggttcgcg
     3841 cagccctgca gcgccgcgaa cgcgatgaac gtgaaaacgc ccgcggcgac gcgggcgttt
     3901 tcgactgcct tgcggccgag cgcggttact tcctgaagaa gaaccaccac actcccgcgg
     3961 cgatgacgat gaccagcagc acccacgccc agtcgtcgga ggcgccggcc ggcatcgcgt
     4021 tgatgcgctg cgcgaggctc gccgcctcgg cgtcggagag cgccgcgacg cgctgcttcg
     4081 cgctcgacgg gtcgacgccg agcgactgca gctggctcgt cacgtcgccg cgggtgagga
     4141 agttgagaac ggtcgtgcga tcggcctgcg atgcagcgct gccgacggcc tggtcggtcg
     4201 tgatgacgcc cgcctgggcg atgtaatacg gggtccagat catcagcgcg acgaggaccc
     4261 ggcagatcgt tcttgtccaa actgatttca ttttagctcc tggctccaaa ggatgaatga
     4321 gggaagggga acaccgcaac gagcatgagc aagaaaggtt ccgttccgtg cgaaatgcgc
     4381 tggccagcgt acgcgagccg atgtgacagg tcgacgtgct gtgacagaat ccgcgacgga
     4441 tggtagagat tcgtcgactt gaagcagcac tgcgcacggc ggtcgcaggc gaagtgcgct
     4501 tcgacgcggg ggcgcgcgcc gcctattcgg ccgacgcgtc gaactaccgg caggtgccga
     4561 tcggtgtcgt gctgccgcgc agcgcggcgg acatcgaggc ggcggtcgcc gtctgccgcg
     4621 agcacggcgc gccgatcctg ccgcgcggct gcggcacctc gctcaacggg cagacggtca
     4681 acgtcgcggt ggtcatcgac tgctcgaagt acctcgaccg catcgtcgcg ctcgatcccg
     4741 cggcgcgcct cgcgcgcgtc gagcccggcg tggtgtgcga cagcctgcgc gaggccgccg
     4801 aagcgcacgg cctcaccttc ggtcccgatc ccgctacgca ctcacgctgc accctgggcg
     4861 gcatgatcgg caacaactcg tgcggcgcgc actcggtgat ggccggcaag accgccgaga
     4921 acatcgcgcg gctggaggtt ttgacctatt ccggttgcag gttttggtgc ggccctacga
     4981 actctgcaga gttcgactcc atacagagga aaggcggaga gcaggccgag atctacctga
     5041 ggctgaagag gctgatcgac aaatacggcg acctggtgcg caagcagttt ccgaaaataa
     5101 ggcgccgcgt gtcgggctac aacctcgacc agctcctgcc ggagaacggc ttcaacgtgg
     5161 cgcgcgccct ggtgggcacc gagggaggct gcgcgatcac gctgcaggcg gaggcgcggc
     5221 tggtcaccag cccgcccagc cgcgtgctgc tggtgatggc gttcgcggac atctatgccg
     5281 ccggcgacgc ggtgccgcag gtcctcgccg ccgggccgat cgcctgcgaa ggcctcgacg
     5341 aggcgatcat cggcgggctg cgcagccgcg gcctgcggct ggaggacatc gcgctgctgc
     5401 cgccgggcaa ggcgtggctg atggtggaat tcggcgccga cacgcagcca gaggccattg
     5461 agaaagcaaa acaactcgat ggaaatttga ttgtcaagaa atccctcatt tcccgcctgt
     5521 ggaccatccg cgagaccggc gcctcggcca ccgcgctcaa cctgcgcggc gaaggcgccg
     5581 acccggtggt cggctgggag gacgccgccg tcgacccgat gcggctgggc gactacctgc
     5641 gggagttcca ggccctcatc gaccgcatgg gctaccgcac ttcgctctac ggccatttcg
     5701 gcgacggctg catccacgcc cgcatcgact ttcgcctgcg cacgcccgaa ggcctcgcgc
     5761 agtggcgccg cttcctcacc gaggctgcgc acctggtggt gaagtacggc ggctcgctct
     5821 ccggcgagca cggcgacggc caggcgaagg ccgagttcct gccgatcatg tacgggcccg
     5881 agctgatgca ggcgttccgc gagttcaagg cgatctggga cccgcgccac cggatgaacc
     5941 cgaacaagct gatcgatccg cgccgcgccg acgaggacct gcggctggga ccgcagtaca
     6001 agccggtcac cctgaatacg aagttcgctt tcacaaccga cgtgggcacc gggtttacgc
     6061 gcgccacaga gcactgcatc ggcatgggca aatgccgctc cgcctccggc ggcaccatgt
     6121 gcccgagcta ccgcgcgacg cgcgaggagc gctattccac gcgcggccgc ggccggctgc
     6181 tcgcggagat gctgcgcggc gaggtgatca ccgacggctg gcgcagcgaa gaggtgaagg
     6241 aggcgctcga ctggtgcctc gcctgcaagg gctgccgcag cgactgcccg acgcacaccg
     6301 acatggccgc ctacaaggcc gagtttctct cgcactacta tgaaacgaag gcgcgaccgc
     6361 tcgtagcgca tctcatgggg cgcatcggcg aatgggctcc gctggcggcg cgcgcatctt
     6421 gggctgtcaa tggtctctct gcggtcctgc ggccccctgg tatcgcgcgg cagcgctctc
     6481 ttccgaaatt caggaaaacc ttccgctcca cgtttgcgtc cggagggcaa cagggtgaaa
     6541 gggttgtgct cttcgacgac acgttcaaca accatttccg cccgcagacc gctcgcgcgg
     6601 cgcagaagct gctggagtcc gccggctgcg cggtggagct gccgcgcgag catctgtgct
     6661 gcggcaggcc gtactacgac tacggcatgc tcgatcgcgc caagggcgcg ctgaatcgcg
     6721 tgctcgaggt gctcgagccg ggagtgccgg tggtgatgct ggagccgggc tgcctttcgg
     6781 tgtttcgtga cgaaatgaag cagctctttc cacgcgacga gcgtgctgct cggctcgagg
     6841 ttttctcgct ttccgaattt ctcctgaaga gaaacttcaa ggtgaaaaac gctgccagcg
     6901 tcgtcgtcca ctcgcattgc catcaaaagg caatttgggg agaaaagagc gatatcgatc
     6961 tgctgcgagc cgcaggctgc agcgtaacgg cgcccgatac gggctgctgc ggcatggcgg
     7021 gctccttcgg ctacaaggcg cagcattacg aggcatcgaa gctgattttc gga
//
LOCUS       DASYJA010000416         2808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2059734, whole genome shotgun sequence.
ACCESSION   DASYJA010000416 DASYJA010000000
VERSION     DASYJA010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2059734"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1597)
                     /locus_tag="VGO02_09110"
     CDS             complement(<1..1597)
                     /locus_tag="VGO02_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEV7477880.1"
                     /translation="MADYKDTLNLPETSFPMRGDLAKREPQWVKEWQENGLYRRLRQV
                     AKGRPRFVLHDGPPYANGDIHIGTAVNKILKDVVVKSKMLAGFDAPYVPGWDCHGMPI
                     EVQIEKKHGKNLPVEQTLKLCRAFATEQIANQKKDFQRLGVLGDWDNPYLTMAPGNEA
                     DEIRVLGKLLEKGYVYRGLKPVNWCFDCGSALAEAEIEYEERKDPAIDVGFAFLENDK
                     LAKAFNTKVAKPGFAVIWTTTPWTLPSNQALNVHPEVTYHLVDTPRGLLILAADLQEA
                     CLKRFSLQGVTVGTCKGKALEKIRFKHPFYDRESPVYLGEYVTLDAGTGIVHSAPAYG
                     VEDFESCRRYGMKDDEILTPVMGDGRFAASLPLFGGLSIWDANKKIVEELERRGVLFH
                     FELYTHSYMHCWRHKTPVILRATTQWFASMDKPIDGETLRARALRGVENTEFFPAWGK
                     ARLHGMIANRPDWTLSRQRQWGVPMPFFLHKETGALHPRTPELLEAVAKRVEQGGIEA
                     WQSVTAEELGVDAKQYEKSRDTLD"
     gene            complement(1590..2504)
                     /locus_tag="VGO02_09115"
     CDS             complement(1590..2504)
                     /locus_tag="VGO02_09115"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEV7477881.1"
                     /translation="MVVTHGSLQSARGRCALTIGNFDGVHRGHRALIDRVTAKARELR
                     LTSCVLTFEPHPREFFARDAAPARLTRLRDKLELMQAAGVKRVHIARFDARFAALSAA
                     RFVDEVLVRGLGMRWLLVGRDFRFGERRGGDFGTLEDAALRHGFRLEEMPDVLFEGER
                     VSSSAVRAALKAGDLKAAERLLGHPYTISGRVAHGAKLGRGLGFPTANIVLRRTAPIA
                     GIFAVEVEGLGRGVASVGRRPTVNPVPIPLLEVHLFDWDRDLYGDHIKVRFLKKLRDE
                     KKYDGLPALQAAIAEDARQAREFFAAHG"
     gene            2539..>2808
                     /locus_tag="VGO02_09120"
     CDS             2539..>2808
                     /locus_tag="VGO02_09120"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HEV7477882.1"
                     /translation="MKTLEDQMAFYAAYHQDGRNKATHFIGVPMIVVSLFIPLAWLKI
                     QVGMFVITGAIVFAAVLLIYYVFLDPVLALATALFTSLFLVTAAWL"
BASE COUNT          442 a          971 c          871 g          524 t
ORIGIN      
        1 catcgagcgt atcgcggctt ttctcgtact gcttggcgtc caccccgagc tcttccgcgg
       61 tcactgactg ccaggcctcg atgccgccct gctccacgcg cttcgccacc gcctcgagca
      121 gctccggggt gcgcggatgc agcgcaccgg tctccttgtg caggaagaac ggcatcggca
      181 cgccccactg ccgctggcgc gagagcgtcc agtccgggcg gttggcgatc atgccgtgca
      241 ggcgcgcctt tccccaggcg ggaaagaact cggtgttctc cactcctcgc aacgccctgg
      301 cccgcagcgt ttctccatcg ataggcttgt ccatcgaggc gaaccactgc gtcgtcgcgc
      361 gcaggatcac cggcgtcttg tggcgccagc aatgcatgta gctgtgcgtg tagagctcga
      421 agtggaacag cacgccgcgc cgctccagct cctcgacgat cttcttgttc gcgtcccaga
      481 tcgacagccc gccgaacagc ggcagcgagg cggcgaacct gccgtcaccc atcaccgggg
      541 tgaggatctc gtcgtccttc atcccgtagc gccggcagga ctcgaagtcc tcgacgccgt
      601 aggccggcgc cgagtgcacg attcccgtgc ccgcatccag ggtgacgtac tcgccgaggt
      661 agaccggcga ctcgcggtcg tagaacgggt gcttgaagcg gatcttctca agcgcctttc
      721 ctttacaagt tcctaccgtt accccttgca aggaaaatct cttcagacac gcttcctgca
      781 agtcggccgc gaggatcagc aggccgcgcg gcgtgtcgac caggtggtac gtgacctcgg
      841 gatggacgtt gagcgcctgg ttcgacggca gggtccacgg cgtggtggtc cagatgacgg
      901 caaagccggg cttggcgact ttcgtgttga atgctttcgc cagcttgtcg ttttcgagaa
      961 acgcaaagcc gacgtcgatc gccggatcct tgcgctcctc gtactcgatc tccgcctcgg
     1021 cgagcgccga gccgcagtcg aagcaccagt tcaccggctt caggccgcgg tagacgtagc
     1081 ctttctcgag cagcttgccg agcacacgga tctcgtcggc ttcgttgccg ggcgccatcg
     1141 tcaggtatgg gttgtcccag tcccccagca cgccgagtcg ctggaaatcc ttcttctggt
     1201 tggcgatctg ctcggtggcg aaggcgcggc acaacttcag cgtctgctcg accggcaggt
     1261 tcttgccgtg cttcttctcg atctgcacct cgatcggcat gccgtggcag tcccagcccg
     1321 gcacgtaggg cgcatcgaag ccggccagca tcttggactt gacgacgacg tccttcagga
     1381 tcttgttgac cgcggtgccg atgtggatgt cgccgttggc gtacggcggc ccgtcgtgca
     1441 ggacgaagcg cgggcggccc ttcgcgacct ggcgcaggcg ccggtagagg ccgttctcct
     1501 gccactcctt cacccactgc ggctcgcgct tggcgaggtc cccgcgcatc gggaaagatg
     1561 tctccggcag attcagcgtg tctttgtaat cagccatgcg ccgcgaagaa ctccctcgcc
     1621 tgccttgcgt cctcggcgat cgccgcctgc agggccggca agccatcgta ctttttctcg
     1681 tcgcgcagct tcttcaggaa gcgcaccttg atgtggtcgc cgtacaggtc gcggtcccag
     1741 tcgaaaagat ggacctcgag cagcggaatc ggcaccgggt tgaccgtcgg ccggcgtccc
     1801 acgctcgcca cgccgcggcc gagaccttcc acctcgacgg cgaagattcc ggcgatcggc
     1861 gccgtgcgcc gcagcacgat gttggcggtg ggaaagccca gcccccgccc gagcttggcg
     1921 ccgtgcgcca cgcgcccgct gatggtgtaa ggatggccca gcagccgctc ggcagccttc
     1981 aggtcgcccg ccttgagcgc ggcgcgcacc gccgagctcg acacccgctc cccctcgaag
     2041 agcacgtccg gcatctcttc gagccggaag ccgtggcgca gcgctgcgtc ctccagcgtg
     2101 ccaaagtcgc cgccgcgccg ctcgccgaag cgaaaatcgc ggcctaccag cagccagcgc
     2161 atgcccaggc cgcgcaccag cacttcgtcc acaaaacgag cagcagaaag cgctgcaaaa
     2221 cgggcgtcga agcgcgcgat gtgcacccgc ttcactccgg cggcttgcat cagctcgagc
     2281 ttgtcgcgca ggcgcgtcag gcgcgcaggc gcggcgtcgc gcgcgaagaa ctcgcgcgga
     2341 tgcggctcga atgtcaggac gcaggaggtc aatcgcagct cgcgtgcctt ggccgtgacg
     2401 cggtcgatga gcgcgcggtg gccgcggtgc acgccgtcaa agttgcctat ggtcagcgca
     2461 caccggcccc gggcgctctg caatgagccg tgcgtgacga ccatggactc ggttaaaacc
     2521 ctgaattata gtgtctttgt gaagacgctc gaagaccaga tggcttttta tgcggcctac
     2581 caccaggacg gccgcaacaa ggccacgcac ttcatcggcg tgccgatgat cgtcgtttcg
     2641 ctcttcatcc cgctcgcctg gctcaagatc caggtcggca tgttcgtgat caccggcgcg
     2701 atcgtgttcg ccgccgtcct cctcatctac tacgtgtttc tcgatccggt gctcgcgctc
     2761 gcgacggcgc tcttcacctc gctctttctc gtgacggccg cctggctc
//
LOCUS       DASYJA010000417         3894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_425458, whole genome shotgun sequence.
ACCESSION   DASYJA010000417 DASYJA010000000
VERSION     DASYJA010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425458"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VGO02_09125"
     CDS             <1..892
                     /locus_tag="VGO02_09125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV7477883.1"
                     /translation="KALSAVTAPFAGRIASVLVEPGQRVRAGTVLFSIESADVLSVRA
                     SLDQARIKLRTAEEVFARQTEMTKRGVGTEAERFDAEMKLREARAEAARAEGNAALAG
                     PGSGTLVSVRTQVDGVVVSVKATVGATVQPGGEPLVEVGDPSALWVLGDVPEGEAAQL
                     AKGAKAEVAISALNVTAAGHVAGIAPRSDPETRRTQFYVELERAPANLRAGLLVRIML
                     RSPAVAGELWLPVTAVLLKDGGRRVVYVEDQGGRFTPRDVEVGDARGGQVRVLRGLQA
                     GERVVMRGALLVDREAEQLL"
     gene            912..>3894
                     /locus_tag="VGO02_09130"
     CDS             912..>3894
                     /locus_tag="VGO02_09130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEV7477884.1"
                     /translation="MLDKLIETCVHRRVATIFTVLIFAVLGVRAYLNTPIEAFPDVTN
                     AQVTVIAQMPGYAPEEIERAVTVPLERALNGTPRMIQLRSESLFGLSLVTLTFDDDAD
                     PFTSRMIVAQRMQEADLPDGVTPRLSPEATPLGEVYQFRMVSDRHDMYELRAELQWTV
                     TRLLKQVPGVADVVSFGGYLKEIHVRPALDRMRAHGITLTDIADALAKANINVGGGFV
                     RVGDQELAVRGIGYLTSVEDIKRVVLKTREGTPVMVGDVAEIVQSNTPRRGAVGYNEQ
                     KEGVEGFALMRRGENPSRVLEALHARIKELNDKVLPRGMRVEPFYDRTLLVGNTLSTV
                     NKNLLHGFLLVVAVAWLFFRSITGSLIVAVVIPLALLGAFIGLYLLKLPANLISMGAI
                     DFGILVDGAVVLVENVLHAVQHERPAQRRDLLRLVIRSAEQVARPTLYAMLIIIAALV
                     PVFTLQQVEGRIFRPLALTYSFALIAALVLALTLVPALCAVGFARGKVAREPRWLEAV
                     RGRYRTMLGNLQPRRSLVAGLAALLLIGGVGMGARLGTEFLPELDEGDILVFVEMPPS
                     IALEASQTMLVEVRKRILTFPEVAQVLSEIGRPEDGTDNENPNMSETFVRLKPRDEWR
                     AGYDKKRVVDEIRELVTEIPGVRYNFSQPIKDNVEEAVSGVRGKVVLKVFGTGMKEMR
                     DSLEQARLKLANVPGIVDLGLYRDATVPQLQIRLDRDSLARAGVPVATANEFVGTALA
                     GKVTTIFWEGERPVPVRVILPLAARDNAKVIGELAVPRPGGGSVPLNELSQLSIATGA
                     ANIYREANSRYLALKFNVEGRDMGSVVKDAIGVVSEGVKLPDGYYFAWGGEFENQARA
                     VKRLEIIIPIALAAVLALLYGATQSGRAALAILFTAPFALTGGAFALAVAGMPLSVSA
                     IVGFIALLGQVSLLGLLLVSAVETKRSQGVPLDTALIEGSVEKLRAILMAALLALFGL
                     LPMAVSTGIGSETQRPFALVIV"
BASE COUNT          559 a         1335 c         1420 g          580 t
ORIGIN      
        1 gaaggcgctg tccgcggtga ccgcgccgtt cgccggccgc atcgcctcgg tgctggtcga
       61 gcccgggcag cgcgtgcgcg ccggcacggt gctcttcagc atcgagagcg ccgacgtgct
      121 gtcggtgcgc gcctcgctcg accaggcgcg catcaagctg cgcaccgccg aggaagtgtt
      181 cgcgcgccag accgagatga cgaagcgcgg cgtcggcacc gaggccgagc gcttcgatgc
      241 cgagatgaag ctgcgcgagg cgcgcgccga ggcggcgcgc gccgagggca acgcggcgct
      301 cgccggcccc ggcagcggca cgctggtcag cgtgcgcacc caggtcgacg gcgtggtggt
      361 ctcggtgaaa gccacggtcg gcgcgacggt gcagcccggc ggcgagccgc tggtcgaggt
      421 cggcgacccg agcgcgctgt gggtgctggg cgacgtgccc gagggcgaag ccgcccagct
      481 ggcgaaaggc gccaaggccg aggtggcgat ctcggcgctc aacgtcacgg ccgccggcca
      541 cgtcgccggc atcgcgccgc gcagcgaccc tgagacgcgc cgcacgcagt tctacgtcga
      601 gctagagcgc gcgccggcca acctgcgcgc cgggctgctg gtgcggatca tgctgcgctc
      661 gccggccgtc gccggcgagc tgtggctgcc ggtgaccgca gtgctgctca aggacggcgg
      721 ccgccgcgtg gtgtacgtcg aggaccaggg gggcaggttc acgccgcgcg acgtcgaggt
      781 tggcgacgcg cgcggcggcc aggtgcgcgt gttgcggggc ctgcaggcgg gcgagcgggt
      841 ggtaatgcgc ggcgcgctgc tggtcgaccg cgaagccgag cagctgctct agccaggcga
      901 gcctccgccc gatgctcgac aagctgatcg agacctgcgt gcatcgtcgc gtcgcgacga
      961 tcttcaccgt tctgatcttc gccgtgctcg gcgtgcgcgc ctacctcaac acgccgatcg
     1021 aggcgttccc cgacgtaacc aacgcgcagg tgacggtgat cgcgcagatg cccggctacg
     1081 cccccgagga gatcgagcgc gcggtcaccg tgccgctcga gcgcgcgctg aacggcacgc
     1141 cgcgcatgat ccagctgcgc agcgagagcc tgttcggcct gtcgctggtc accctcacct
     1201 tcgacgacga cgcggacccg ttcacctcgc gcatgatcgt cgcgcagcgc atgcaggagg
     1261 ccgacctgcc cgacggggta acgccgcggc tctcgccgga ggcgacgccg ctcggcgagg
     1321 tgtaccagtt ccgcatggtg agcgaccgcc acgacatgta cgagctgcgc gccgagctgc
     1381 agtggaccgt gacccggctg ctgaagcagg tgccgggggt cgccgacgtg gtgagcttcg
     1441 gcggctatct caaggaaatc catgtccgcc cggcgctcga ccggatgcgc gcgcacggca
     1501 tcacgctgac cgacatcgcc gacgcgctcg ccaaggcgaa catcaacgtg ggcggcggct
     1561 tcgtgcgcgt cggcgaccag gagctcgccg tgcgcggcat cggctacctc acctcggtcg
     1621 aggacatcaa gcgggtggtg ctgaagactc gcgagggcac gcctgtgatg gtcggcgacg
     1681 tcgccgagat cgtgcagtcg aacacgccgc ggcgcggcgc ggtcggctac aacgagcaga
     1741 aagagggggt cgagggcttc gccctgatgc gacgcggcga gaacccctcg cgcgtgctcg
     1801 aggccctgca cgccaggatc aaggagctga acgacaaggt cctgccgcgc ggcatgcgag
     1861 tcgagccgtt ctacgaccgc accctgctgg tcggcaacac gctgtcgacg gtcaacaaga
     1921 acctgctgca tggcttcctc ctggtggtgg cggtggcgtg gcttttcttc cgcagcatca
     1981 ccggctcgct tatcgtcgcc gtagtgattc cgctcgcgct gctcggcgcc ttcatcggcc
     2041 tgtaccttct caagctgccc gccaacctga tctcgatggg cgccatcgac ttcggcatcc
     2101 tggtcgacgg cgcggtggtg ctggtggaaa atgtgctgca cgcggtgcag cacgagcgcc
     2161 cggcgcagcg ccgcgacctg ctgcgcctgg tgatccgctc ggcggagcag gtggcgcgcc
     2221 cgacgctgta cgcgatgctg atcatcatcg ccgcgctggt gccggtgttc acgctgcagc
     2281 aggtcgaggg gcgcatcttc cggccgctgg cgctcaccta ctcctttgcg ctgatcgcgg
     2341 cgctggtcct cgcgctcacc cttgtgccgg cgctgtgcgc ggtcggtttc gcgcgcggca
     2401 aagtggcgcg cgagccgcgc tggctggagg cggtgcgcgg ccgctaccgc accatgctgg
     2461 gcaacctcca gcccaggcgc agcctggtag ccgggctggc tgcgctgctg ctcatcggcg
     2521 gcgtcggcat gggcgcgcgg ctgggcaccg agttcctgcc ggagctcgac gagggcgaca
     2581 tcctggtgtt cgtcgagatg cctcccagca tcgcgctcga ggcgtcgcag accatgctgg
     2641 tcgaagtgcg caagcgcatc ctgactttcc ccgaggtggc gcaggtgctg tcggagatcg
     2701 gccgccccga ggacggcacc gacaacgaga acccgaacat gagcgagacc ttcgtgcgcc
     2761 tgaagccgcg cgacgaatgg cgcgcgggct acgacaagaa gcgcgtggtg gacgagatcc
     2821 gcgagctggt gaccgagatc ccgggcgtgc gctacaactt ctcgcagccg atcaaggaca
     2881 acgtggagga ggcggtctcc ggcgtgcgcg gcaaggtggt gctcaaggta ttcggcaccg
     2941 gaatgaagga gatgcgcgac tcgctcgagc aggcgcggct caagctcgcc aacgtgccgg
     3001 gcatcgtcga cctcggcctg taccgggacg ccaccgtgcc gcagctgcag atccgcctcg
     3061 accgcgactc gctggcgcgc gccggggtgc cggtggccac cgccaacgag ttcgtcggca
     3121 ccgccctcgc cggcaaggtc accaccatct tctgggaggg cgagcggccc gtcccggtgc
     3181 gggtgatcct gccgctcgcg gcgcgcgaca acgccaaggt gatcggcgag ctcgcggtgc
     3241 cgcgccccgg cggcggcagc gtcccgctga acgagctcag ccagctgtcg atcgcgaccg
     3301 gcgccgcgaa catctaccgc gaggccaaca gccgctacct ggcgctcaag ttcaacgtcg
     3361 agggccgcga catgggctcg gtggtgaagg acgccatcgg cgtcgtgtcc gagggagtca
     3421 agctgcccga cggctactac ttcgcctggg gcggcgagtt cgagaaccag gcgcgcgcgg
     3481 tcaagcggct ggagatcatc attccgatcg cgctcgccgc ggtgctcgcg ctgctctacg
     3541 gcgccaccca gtcgggccgc gccgcgctcg ccatcctgtt cacggcgccg ttcgccctga
     3601 ccgggggcgc attcgccctg gcggtcgcgg gcatgccgct gtcggtgagc gcgatagtgg
     3661 gctttattgc acttctgggt caggtctcgc tgctggggct gctgctggtg agcgcggtcg
     3721 agaccaagcg cagccagggg gtgccgctcg acacggcgct gatcgaaggc agcgtggaga
     3781 agctgcgcgc gatcctgatg gcggcgctgc tcgcgctctt cggcctgctg ccgatggccg
     3841 tatcgacggg catcggcagc gagacgcagc gcccgttcgc gctggtgatc gtcg
//
LOCUS       DASYJA010000418         6814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3151473, whole genome shotgun sequence.
ACCESSION   DASYJA010000418 DASYJA010000000
VERSION     DASYJA010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6814
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3151473"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2323
                     /locus_tag="VGO02_09135"
     CDS             <1..2323
                     /locus_tag="VGO02_09135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HEV7477885.1"
                     /translation="APAAPETKPEPAAPAPQAAPEAKPEAAAEAKPEAAAEAKPEAAA
                     EAKPEAAAEAKPEAKPETAPDAKPEPKIEAQPLPATAPAAAAPAAAAAPSMYEVRKGD
                     TLGAIARQHAPAGVTHNQMLIALFRNNPDAFIRENINLVRAGQILNIPDADAVASIEQ
                     TDANRLVSAHIADFNDYRSRLAGAPAAAESAPGQRESAGRIEPKPAAAPPAGTQDQLK
                     LSKADPQKPSASGSRAAQGDDAVARERALKEAQSRVTDLEKNVADLQKLLELKNQQLA
                     ELEKKAGGKPAAAAPAPAPAPAAKAPEPAKPAAPAPAPAPAAKAPEPAKPAAPPSPPV
                     VAQAPEPAKPAAEPAKPAAEPAKPGAEPAKPAAPAAPAPAAKAPETKPAPKPEPSLLD
                     EFLGNEFALYGLGFVVVLLVGYGVFAWSRKKKAQARFQDSVLGATSGAASVFGTPSAA
                     PVSAGPGASQASVSQPAGMDAEEVDPIAEADVYMAYGRDAQAEEILKEALQKDNKRTA
                     VHVKLLEIYANRKDTQGFEQSAVQLKSLTNGAGPEWEKAAALGRSIDPQNGLYGGAGG
                     GAAAAPAAAAPALDFDLGGASSTQGAMPDISLDAPTQEPSASGGSVDFDLGGTPAAAT
                     PPTDFTPEGTLIMGAQTPESTASGLDFDLGSPKTEVPAAPAESPPSDGGLDFDLKLDD
                     MPGAKSEPSAAPATPDLSEISLDLGAPAEPSAPSGGGSDPKWQEVATKLDLAKAYEEM
                     GDKDGARELLNEVMKDGDGAQKGQAQQLLAKLG"
     gene            2335..3084
                     /gene="truA"
                     /locus_tag="VGO02_09140"
     CDS             2335..3084
                     /gene="truA"
                     /locus_tag="VGO02_09140"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEV7477886.1"
                     /translation="MALGLEYDGSRFLGWQTQPGGGAVQDALEPALAQIAGEPVNVTC
                     AGRTDRGVHARAQVVHFDTGAQRPASAWVRGVNALLPDTVAVLWSQPVADEFHARFGA
                     FARTYRYELLNRAVRPALAATRVGWFHLPLDAERMREAAALLVGEHDFSAFRSAECQA
                     KSPVRTLHSLEIERHGERIDFVLRANAFLHHMVRNIVGTLVYVGKGSLSPFQVKEVLL
                     SKDRSKAAPTFGPEGLYLEKIEYEAKWSLPQ"
     gene            3066..3701
                     /locus_tag="VGO02_09145"
     CDS             3066..3701
                     /locus_tag="VGO02_09145"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEV7477887.1"
                     /translation="MEPSSVRTRVKICGITRPGDARAAAEAGADAIGLVFYPKSPRYL
                     STERAMEIRDALPPFVQTVALFVNADAAQVAQVLGRVRPGMLQFHGEETPQFCAQFGV
                     PYVKACRVRPGVDLLEYLRPFSSAAAWLLDSFVEAYGGVGERFDWSLVPRGLERPLIL
                     SGGLECGNVAEAVRKLRPWGVDVSSGVESAKGIKDAAKIAAFISEVRNADV"
     gene            3694..4887
                     /gene="trpB"
                     /locus_tag="VGO02_09150"
     CDS             3694..4887
                     /gene="trpB"
                     /locus_tag="VGO02_09150"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV7477888.1"
                     /translation="MYDLPDARGHFGPYGGVFVAETLSAALDELRTAYEQARQDPAFD
                     AEFRYELKHYVGRPSPVYFAKRLTEKCGGARIYIKREDLNHTGAHKINNTIGQALLAR
                     NMKKPRVIAETGAGMHGVATATVAARYGMECVVYMGVEDVKRQAANVHRMGVLGARVV
                     PVESGSRTLKDALNEAMRDWVTNVDSTFYIIGTVAGPHPYPMMVRDFQKVIGEESIVQ
                     MQELAGRQPDAVIACVGGGSNAMGIFYPYIPHGKVKLIGVEAAGEGLETGKHAASLCA
                     GRPGVLHGNRTYLLQDANGQITETHSVSAGLDYPGVGPEHAWLKDSKRAEYVAVTDAE
                     ALAAFHELCRSEGIIPALESAHAVAQAMKMAPSMPKDAILLVNLSGRGDKDMHTVAER
                     SGTKL"
     gene            4887..5684
                     /gene="trpA"
                     /locus_tag="VGO02_09155"
     CDS             4887..5684
                     /gene="trpA"
                     /locus_tag="VGO02_09155"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEV7477889.1"
                     /translation="MSRIQGCFEALAKAKRKALIPYITAGDPNPALTVPLMRALVESG
                     SDILELGVPFSDPMADGPVIQRSGERALKHGVGLVDVLSMVKDFRKSDDKTPVVLMGY
                     ANPIEAMGMEKFAASAKAAGIDGVIVVDYPPEECLEFSALMKRSDVDPIFLLAPTSTK
                     KRIDEVARSGSGYLYYVSLRGVTGAANIDLSDVAAHIPQIRRATKLPIGVGFGIRDAE
                     SARRVAQTADAVVIGSRIIQEIEGGRPEEAVMRVKNFLKPIRQALDA"
     gene            5681..6565
                     /gene="accD"
                     /locus_tag="VGO02_09160"
     CDS             5681..6565
                     /gene="accD"
                     /locus_tag="VGO02_09160"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEV7477890.1"
                     /translation="MSWLQKLLPPKIQRTGSGARKTVPEGLWTKCASCDAVLYSTDLE
                     KNLNVCPKCGHHERLGARPRLDALLDAEGRFEIGAEVLPVDTLKFKDSRRYPERLAEA
                     QEQTAEGDALVVMQGSIKALGVVCAAFEFDFMGGSMGSVVGERFVRGVRVAIEQKLPF
                     VCVTASGGARMQEGLLSLAQMAKTTASLTDLAERKLPFVSVLTDPTMGGVSASFAMIG
                     DVVIAEPKALIGFAGPRVIEQTVRQKLPEGFQRAEFLLEKGAIDMIVDRRQLRDKLHL
                     LLSLLLKLPAEATTTATA"
     gene            6562..>6814
                     /locus_tag="VGO02_09165"
     CDS             6562..>6814
                     /locus_tag="VGO02_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477891.1"
                     /translation="MTRLAALVRVSNQVAATPSRLAKIRELADFLRPLERAEIEIALP
                     YLSGDIRQGRLTVGFAALQAARRSPAPSPTLGLRDVDRAF"
BASE COUNT         1145 a         2379 c         2360 g          930 t
ORIGIN      
        1 ggcgcctgcc gcgccggaga ccaaacccga gcccgcggcg ccggcgccgc aagccgcccc
       61 cgaggcgaag ccggaagccg cagccgaggc caagccggaa gccgcagccg aggcgaagcc
      121 ggaagccgca gccgaggcca agccggaagc cgcagccgag gcgaagccgg aagccaagcc
      181 agagaccgcg ccggatgcca agccggagcc caagatcgaa gcgcagccgc tgccggccac
      241 ggcacctgcg gcagccgcgc cggccgccgc cgcagcgccg agcatgtacg aggtcaggaa
      301 gggcgacacg ctgggtgcga tcgcgcgcca gcatgcgcct gccggcgtga cgcacaacca
      361 gatgctgatc gcgctgttcc gcaacaatcc ggatgcgttc atccgcgaga acatcaacct
      421 ggtgcgcgcc ggccagatcc tcaacattcc cgacgccgac gccgtcgcct cgatcgagca
      481 gaccgacgcg aaccgcctgg tgtcggcgca tatcgccgat ttcaacgact accgcagccg
      541 cctcgccggc gcgccggccg ccgccgaaag cgcgccgggc cagcgcgaat ccgccggccg
      601 tatcgagccc aagcccgcgg cggcgccgcc cgccgggacg caggatcagc tgaagctgtc
      661 caaggccgac ccgcagaaac cgtccgccag cggctcgcgc gcagcgcagg gcgacgatgc
      721 cgtggcgcgc gagcgtgctc tgaaggaagc gcagtcccgc gttaccgacc tcgagaagaa
      781 cgtcgccgac ctgcagaagc tgctcgagct gaagaaccag cagctcgccg agctggagaa
      841 gaaggccggc ggcaagcccg cggcggccgc gccggcgccc gctcccgctc cggccgcgaa
      901 ggcgcccgag cccgccaaac ctgctgcccc cgctcccgca ccggcgcctg cggcaaaggc
      961 ccctgaaccc gcgaagcccg ctgcgccgcc atccccgcct gtagtggcgc aggcgccgga
     1021 gcctgcgaag cccgctgccg aaccggccaa gccggccgcc gagccggcca aaccgggtgc
     1081 cgaaccggca aagccggcgg cgcccgccgc acccgcgccc gccgccaagg cgcccgagac
     1141 caagcccgcg cccaagccgg agcccagcct gctcgacgag ttcctgggca acgagtttgc
     1201 cctctatggc ctgggcttcg tcgtcgtcct gctggtcggc tacggcgtgt tcgcctggag
     1261 cagaaaaaaa aaggcccagg caaggtttca agatagcgta ctaggcgcga cctccggcgc
     1321 ggcctcggtg ttcggcacgc cttccgccgc gcccgtctcg gcgggcccgg gcgcctcgca
     1381 ggcgtcggtc agccagcccg ccggcatgga tgcggaggaa gtcgacccga tcgcggaagc
     1441 cgacgtctac atggcgtacg gacgcgatgc gcaggccgag gagatcctca aggaagctct
     1501 gcagaaggac aacaagcgca cggcggtgca cgtcaagctg ctcgagatct acgccaaccg
     1561 caaggacacg caggggttcg agcagagcgc ggtccagctg aagagcctga ccaacggcgc
     1621 cgggccggag tgggaaaagg ccgcggcgct cggccgctcg atcgatccgc agaacgggct
     1681 gtacggcggc gccggcggcg gcgccgctgc ggcgcctgcg gcggccgcac cagcgctcga
     1741 cttcgacctg ggcggggcga gctccaccca gggggcgatg cccgacattt cgctcgatgc
     1801 gccgacgcag gaaccttccg cttccggcgg cagcgtggac ttcgacctcg gcggcacacc
     1861 ggccgccgcc acgccgccga ccgacttcac gcccgagggc acgctcatca tgggcgcgca
     1921 gacgcccgaa tccaccgcgt cgggcctgga tttcgacctg ggttcgccca agacagaagt
     1981 gcctgcggcc ccagccgagt cgccgccatc cgacggcggg ctggacttcg atctcaagct
     2041 cgacgacatg cccggcgcga agtccgagcc gtcggccgcg ccggccacgc ccgacctgtc
     2101 cgagatcagc ctcgacctcg gcgcgccagc cgagccctcc gcgccgtcgg gcggcggctc
     2161 ggatccgaaa tggcaggaag tcgccaccaa gctcgacctg gccaaggcct acgaggaaat
     2221 gggcgacaag gacggcgcgc gcgagctgct gaacgaagtc atgaaggacg gcgacggagc
     2281 gcagaagggc caggcgcagc agctgctcgc caagctaggc taggtcctat caggatcgct
     2341 ctaggactcg agtacgacgg cagccgcttt ctcggctggc agacccagcc cggcggcggc
     2401 gcggtgcaag acgcgctcga gccggccctc gcccagatcg ccggcgagcc agtgaacgtg
     2461 acctgcgcgg gcaggaccga ccgcggcgtg catgcgcgcg cgcaggtcgt gcacttcgat
     2521 accggcgcgc agcgcccggc atcggcctgg gttcgagggg tcaacgcgct gctgccggac
     2581 accgtggcgg tgctctggtc gcagccggtg gccgacgagt tccacgcccg cttcggcgcg
     2641 ttcgcgcgca cctaccgcta cgagctgctc aaccgtgccg tgcgtccggc gctcgccgcg
     2701 acccgggtcg gctggtttca cctgccgctc gatgccgagc gcatgcgcga ggcagcggcg
     2761 ctgctcgtcg gcgagcatga tttctccgcc ttccgctccg ccgagtgcca ggcgaagtcg
     2821 cccgtgcgca cgcttcactc gctcgagatc gaaaggcacg gcgagcgcat agacttcgtg
     2881 ctgcgcgcca atgccttcct ccaccacatg gtgcgcaaca tcgtcggtac cctggtctac
     2941 gtcggcaagg gcagcctttc gccgttccag gtgaaggagg tcctgctgtc gaaagaccgc
     3001 agcaaggcgg cgccgacctt cggcccggag ggcctctatc tggagaaaat agaatacgag
     3061 gcgaaatgga gccttcctca gtgaggacgc gggtcaagat ttgcggcatc acccggccag
     3121 gagacgcgcg tgccgccgcc gaggccggcg ccgacgccat cggcttggtg ttctatccaa
     3181 agagcccgcg ctatctctcg acggagcgcg cgatggaaat ccgcgacgcg ctgccgccgt
     3241 tcgtgcagac ggtggcgctg ttcgtcaacg ccgacgcggc gcaggtggcg caggtgctcg
     3301 gccgggtcag gcccggcatg ctgcagttcc acggcgagga gacgccgcag ttctgcgcgc
     3361 agttcggcgt gccttacgtg aaggcctgcc gggtgagacc gggggtcgat ttgctagaat
     3421 acctgcggcc tttttcgagc gcggcggcgt ggctgctcga cagcttcgtc gaggcgtacg
     3481 gcggggtcgg cgagcgcttc gactggtcgc tggtgccgcg cgggctcgag cggcccctca
     3541 tcctctccgg cgggctcgag tgcggcaacg tcgccgaagc ggtccgcaag ctgcgtccgt
     3601 ggggcgtcga tgtgtcgagc ggcgtcgagt cggcgaaggg catcaaggac gcagcgaaga
     3661 tcgcggcgtt catctcggag gtcaggaatg cggatgtatg acttgcccga tgcgcgcggc
     3721 cacttcggcc cctacggcgg cgtcttcgtc gccgagacgc tgagcgcggc gctcgacgag
     3781 ctgcgcaccg cctacgagca ggcgcgccag gatcccgcgt tcgacgccga gttccgctac
     3841 gagctcaagc actacgtggg gcgcccgagt ccagtttatt ttgcaaaaag attgacggaa
     3901 aagtgcggcg gcgcgcgcat ttacatcaag cgcgaggacc tgaaccacac cggcgcgcac
     3961 aagatcaaca ataccatcgg ccaggcgctg ctcgcgcgca acatgaagaa gccgcgcgtc
     4021 atcgccgaga ccggcgccgg catgcacggc gtcgccacgg cgaccgtggc cgcgcgctac
     4081 ggcatggagt gcgtcgtgta catgggcgtc gaggacgtca agcggcaggc ggcgaacgtg
     4141 caccgcatgg gcgtgctcgg cgcaagagtg gtgccggtcg agtccggctc gcgcacgctg
     4201 aaggacgcgc tcaacgaggc gatgcgcgac tgggtcacca acgtcgacag caccttctac
     4261 atcatcggca ccgtggccgg cccgcacccg tacccgatga tggtgcgcga tttccagaag
     4321 gtgatcggcg aggagagcat cgtgcagatg caggagctcg ccggccgcca gcccgatgcg
     4381 gtcattgcct gcgtcggtgg cggctccaac gccatgggca tcttctatcc atacatcccg
     4441 cacggcaagg tgaagctgat cggcgtcgag gcggcgggag agggcctcga gacggggaag
     4501 cacgcggcct cgctgtgcgc cggcaggccc ggcgtgctgc acggcaaccg cacctatctg
     4561 ctgcaggacg cgaacggcca gatcaccgag acgcactcgg tatcggccgg gctcgactat
     4621 cccggcgtgg ggcccgagca cgcctggctg aaagacagca agcgcgccga gtacgtggcg
     4681 gtcaccgacg ccgaagcgct cgcggctttc cacgagctgt gccgcagcga aggcatcatc
     4741 ccggccctgg agtcggcgca cgccgtggcg caggcgatga agatggcgcc gtcgatgccg
     4801 aaggacgcga tcctcctggt caacctctcc ggccgcggcg acaaggacat gcatacggtc
     4861 gccgagcgct ccgggacgaa gctttgatgt cgcggatcca gggctgcttc gaggcgctcg
     4921 ccaaggcgaa gcgcaaggcg ctcatcccct acatcactgc cggcgatcct aatcccgcgc
     4981 ttaccgtgcc gctgatgcgg gcgctggtgg aatccggctc cgacattctc gagctgggcg
     5041 tgccgttctc cgatccgatg gccgacggcc cggtgatcca gcgctccggc gaacgggcgc
     5101 tgaagcatgg cgtaggcctg gtcgacgttc tctcgatggt gaaggatttc cgcaaaagcg
     5161 acgacaagac cccggtcgtg ctgatgggct atgccaaccc gatcgaggcg atgggcatgg
     5221 agaagttcgc cgcttcggcg aaggccgcgg gcatcgacgg cgtcatcgtg gtcgactatc
     5281 cgcccgagga gtgcctcgag ttctcggccc tcatgaagcg cagcgatgtc gacccgattt
     5341 tcctgctggc tccgacctcg accaagaagc gcatcgacga agtcgcgcgc agcggcagcg
     5401 gctacctgta ctacgtatcg ctgcgcggcg tgaccggcgc cgccaacatc gatctttcag
     5461 atgttgcagc ccacattccg cagatccgca gggcgacgaa gctgccgatc ggcgtcggct
     5521 tcggcattcg cgacgccgag tcggcgcggc gcgtcgccca gaccgcggac gcggtggtca
     5581 tcggcagccg gatcatccag gagatcgaag gcgggcggcc ggaggaggcg gtgatgcggg
     5641 tcaagaactt cctgaaaccc atccgacagg ccctggacgc atgagctggc tgcaaaaact
     5701 cctgccgccc aagatccagc gcaccggcag cggcgcgcgc aagaccgtgc ccgaagggct
     5761 gtggaccaag tgcgcgtcct gcgacgcggt gctgtacagc accgacctgg agaagaacct
     5821 caacgtctgc ccgaagtgcg ggcaccacga gcgcctcggc gcgcggccgc gcctcgacgc
     5881 gctgctcgac gccgagggcc gcttcgagat cggcgccgag gtgctgccgg tcgatacgct
     5941 caagttcaag gacagccgcc gctatcccga gcggctggcg gaggcgcagg agcagaccgc
     6001 cgagggcgac gcgctggtgg tgatgcaagg ctcgatcaag gcgctcggcg tggtgtgcgc
     6061 cgcgttcgag ttcgacttca tgggcggctc gatgggctcg gtggtgggcg agcgcttcgt
     6121 gcgcggggtg cgcgtggcca tcgagcagaa gctgccgttc gtctgcgtca ccgcctcggg
     6181 cggcgcgcgc atgcaggagg ggctgctttc tctcgcgcag atggccaaga ccaccgcgtc
     6241 gctcaccgat ctcgccgagc gcaagctgcc tttcgtctcg gtgctgaccg atccgaccat
     6301 gggcggcgtt tcggcgagct tcgccatgat cggcgacgtg gtaatcgccg agccgaaggc
     6361 gctgatcggc tttgccgggc cgcgcgtcat cgagcagacc gtgcggcaga agctgcccga
     6421 gggcttccag cgcgccgagt tcctgctgga gaagggcgcg atcgacatga tcgtcgaccg
     6481 ccgccagctg cgcgacaagc tgcacctgct cctttcgctg ctgctgaagc tgcccgccga
     6541 ggccacgacg accgccactg cgtgacgcgt cttgccgcgc tggttcgcgt ctcgaaccag
     6601 gtcgctgcaa cgccgagccg cctcgccaag atccgcgaac tggcggattt cctgcggccg
     6661 ctcgagcgtg ccgaaatcga gatcgcgctg ccttatcttt ccggcgacat ccggcaaggc
     6721 cggctcacgg tcggctttgc agcgctgcag gcggcgcggc gctcgccggc gccgtcaccg
     6781 acgctcggcc tgcgcgacgt cgaccgcgcg ttcg
//
LOCUS       DASYJA010000419         3297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1333405, whole genome shotgun sequence.
ACCESSION   DASYJA010000419 DASYJA010000000
VERSION     DASYJA010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333405"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1634)
                     /locus_tag="VGO02_09170"
                     /pseudo
     CDS             complement(1..1634)
                     /locus_tag="VGO02_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814283.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            complement(1754..2641)
                     /locus_tag="VGO02_09175"
                     /pseudo
     CDS             complement(1754..2641)
                     /locus_tag="VGO02_09175"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2694..>3297)
                     /locus_tag="VGO02_09180"
     CDS             complement(2694..>3297)
                     /locus_tag="VGO02_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEV7477892.1"
                     /translation="FGKLTLADPDSVDLTNLQRQILYGTSSVGSPKVDEARKSLHAFN
                     PEVQVVALQKKLLKEDLDRAVADCSVVLDCSDNFATRHAVNRACVRHRKPLVSGAAIR
                     FDGQLAVFDLRKADSACYACLYPEDGEVEEVQCSTMGVFAPLTGVIGAMQAMEAIKLV
                     AGAGEAPAGLLIFDAARTEWRRLGLKRDPACPVCGNQGRM"
BASE COUNT          504 a         1076 c         1106 g          611 t
ORIGIN      
        1 gcgtcgacca gccggtgcag cacgcggatc agcttctcga cgtcggccat gtgcaggccg
       61 accgtgggtt cgtcgagcac atagagagtg gaagttgtat ttgttttcga taattcataa
      121 accaatttga tgcgctgtgc ttcccctccg gagagggtgg ggctttgctg gccgagcgtc
      181 agatagccca gccccacgtc ctgcagcagg cgcaggcagc ggtggatcga ggggtgcgcg
      241 gcgaagaact ccaccgcctc gtcgatgctc atgccgagca cttcgcccac ggtcttgccg
      301 cgcagccgca cgctcagcgt ctcggggttg aagcgcgcgc cgctgcagcc ctcgcaggcc
      361 acgcgcacgt cgggcaggaa ggacatctcg atcttcttca tcccctggcc atcgcacgct
      421 tcgcagcgcc cgcccttggt gttgaacgag aagcggctcg cgctccagcc gcgcacgcgc
      481 gcctcgctgg tgtcggcgaa caggcgccgg atcgcgtccc agaagccgac gtaggtggcc
      541 gggcaggagc gcggcgtctt gccgatcggc gtctggtcga cctccagcac gcgctcgacg
      601 cgctggcggt tgcggatggc tttgcagccg accagggtct tctccgacaa agatctgaac
      661 aggatgtcgc gggcgagcgt cgacttgccg gagccggaca cgccggtcac cgcgatcagc
      721 cggccgagcg ggaagcgggc gtcgatgcct ttgaggttgt gcagcttggc tttttcaact
      781 tccaaaaagg aaacatcctt cgaaatgcct cttctgggct gcaacgggtg cttcagggga
      841 tttttcagga agcgcccggt gatcgaatca gggtgccgca tgagctcctc ggcgtcgcct
      901 tcggccacca cgaagccgcc gagcttgccg gcgccggggc caaggtcgag cacatggtgg
      961 gcgcggcgga tggtgtcctc gtcgtgctcg accacgacca gcgagttgcc cttcgcctcg
     1021 agcttctcca gcacgtcgag gaggatcttg ttgtcgcggt ggtgcaggcc gatggtcggc
     1081 tcatcgagga tgtagcagac gccgcgcagg ttggagccga gctgcgccgc gaggcggatg
     1141 cgctgcgcct cgccgccgga gagcgtcggc gccgagcggt cgagcgtcag gtagccgagc
     1201 ccgacttcct ggaggaaacc caggcgggac ttcagctcgg ccacgatatc gcgggcgatt
     1261 tccgcttctc ttccctgggt ttttattttt gagaagagat tcctgagcga ctcgatcggc
     1321 tcgcccgcgt attgcgcgat gttccgatcg cgccagcgca ccgcgagcgc ctcggggttg
     1381 agacggcttc ccttgcacgc ttcgcactcc tggtcgacct cgagccactc gatccacgag
     1441 tcgagcacgt tgtcctcggc gccggtgcgc gcgcgctcgt cggaccagtc gacctcgtcg
     1501 atgttgagcc cggtgccgta gcacttggcg caccagccgt gcttcgagtt gtaggagaac
     1561 aggcgcgggt cgagctcggc gaagctgcgg ccgcaggagc ggcaggcgcg cttggtggag
     1621 aacacctgga tctgcttgcc gcgcagtttg cccgcttgga tgtgcaccac gcccttgccg
     1681 aactcgagcg ccgtgcgcgc cctttcggcc cgcgcggcga gggaaaggtc cacagctgct
     1741 cggcgacctt cggcgcgtcg cgcttggcga ccgcccaggc gctcggcgga ttcatgatca
     1801 gcgcgccctt gccggcgacc agccatctgt tgttggaggc gtcgtcccag gcgaagacgt
     1861 cgcccggcag caccgccacc agcttcttca tgtactcgag cacctcgcgc accgcgtcgg
     1921 acttgacggt gacgttgccc ttggcgtcga ccagcaccgc gccgtaggcg ctgaacaggg
     1981 cgcccatcgt gtcgaccgaa tccggcgtct gccccaggcc gatgccgaag ggcaccccgg
     2041 ccttgtggca cttctgcgcc gcggtgagga acccttccca ggtccaggcc tccgacagcg
     2101 ccttgtcggg cgcggcgccg gcgggataca tcttctgcac gtcgaggccg gcgtgctgct
     2161 tgagcagatc gatgcgcccg caggggccct tgatctggct gcccggggtg gccgggatgg
     2221 caatccactt gccgtcagac ttggcgaggt attcgatgag aggcgagacc gcgccattgg
     2281 cggcgacgac cgccttcatc acgtcgtcga ccggctcgag caggtgtccc ttgtccgccg
     2341 gctgccaggt cgggaacgcc agcatgtcgt ggccggcctt ggcttgcgcc tcggcggcga
     2401 tggtcagcat gttcttgttg ccctgcgaag ggatgtagtc gatcttgatg tcgaccttct
     2461 ccttggcggc ccactcgttg cacatcgcgg tgagcacgtt gttggcgccg gggacccagt
     2521 ggtcccagaa gcccaccgac agcgatcccg cggcgtgcga agtgcgcagg tagggagctg
     2581 ctagggtgac tgctgcggcg ctgccgatga agcgacgtcg ggtgaatttc cttgaagcca
     2641 tggtctcgtt cctcctcagg ttgagactag ccaagcgtga aattccgtcg tgcttacatc
     2701 ctaccttgat tcccgcagac cgggcaggcc gggtcccgct tcagccccag cctgcgccac
     2761 tcggtcctgg ctgcgtcgaa gatcagcagg ccggcgggtg cctcgcccgc cccggcgacg
     2821 agcttgatcg cctccatcgc ctgcatggcg ccgatgacgc cggtgagcgg cgcgaacacg
     2881 cccatcgtcg agcactgcac ttcctccact tcgccgtcct cggggtagag gcaggcatag
     2941 caggccgaat cggctttgcg caggtcgaag acggcgagtt gcccgtcgaa gcggatcgcc
     3001 gcaccggaaa ccagcggctt gcgatgcctc acgcacgcgc ggttcaccgc gtggcgcgtc
     3061 gcgaagttgt cgctgcagtc aagcacgacg ctgcagtcgg cgaccgcgcg atccaggtct
     3121 tccttgagga gcttcttctg tagagcaacg acctgaactt ccggattgaa ggcgtggagc
     3181 gacttgcgcg cttcgtctac tttcggcgaa ccgaccgagc tcgtgccgta caggatctgg
     3241 cgttgcagat tggtgaggtc gacgctgtct ggatcggcga gcgtcagctt gccgaag
//
LOCUS       DASYJA010000420         2617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2423008, whole genome shotgun sequence.
ACCESSION   DASYJA010000420 DASYJA010000000
VERSION     DASYJA010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2423008"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..860)
                     /gene="rsmI"
                     /locus_tag="VGO02_09185"
     CDS             complement(33..860)
                     /gene="rsmI"
                     /locus_tag="VGO02_09185"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV7477893.1"
                     /translation="MPAGTLYVVATPIGNLADASPRAIEALRSAQVIACEDTRTTRTL
                     LARYAIEARSVALHEHNERAATGKLIALLLEGKNIALVSDAGTPALSDPGALLVEQAH
                     RAGIRVSPLPGPSAAAAALSVSGFATDRFLFVGFLPAAATARRKALQALELPWPVVLY
                     EAPHRIAATLEDLRTRFGEQREVLIARELTKKFEEVARLSLGQAAGWLAAQPHRQQGE
                     FVLVLAPGGEAPPAIDADRVLAALLEAVSPSEAAKLAARITGAPRNELYRKALARAK"
     gene            914..1252
                     /locus_tag="VGO02_09190"
     CDS             914..1252
                     /locus_tag="VGO02_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEV7477894.1"
                     /translation="MNWSGARAEDLCAQVLQGAGLRLVARNWRCRLGEIDLIADEGGT
                     LVFAEVRMRSGGGFGGAAESVTAAKRGRLLAAARLYLSRRPEAPTRFDVLLVDGTAGR
                     VEWIRDAFGE"
     gene            1320..1610
                     /locus_tag="VGO02_09195"
     CDS             1320..1610
                     /locus_tag="VGO02_09195"
                     /inference="COORDINATES: protein motif:HMM:NF020376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HEV7477895.1"
                     /translation="MQSSPLAGFRYHAAAEVWQELRIGDLLELQREPDNPHDANAVSV
                     SWRGRKLGYLPRRENAAASWSLDRGQRLSARISRLAQHPNPARRIEVEVYAE"
     gene            1669..2262
                     /locus_tag="VGO02_09200"
     CDS             1669..2262
                     /locus_tag="VGO02_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEV7477896.1"
                     /translation="MLAARVAAQFAESARLKLEAAQSLVGPLVRAGTLLAEALRQGGK
                     VLACGNGGSAADAQHFAAELVNRFEMERPPLAGIALTTDTSTLTSIANDYAYLQVFSK
                     QVRALGRRGDVLLAISTSGNSGNVVEAISAAHELGLRVIALTGNGGGKMAAMLGPDDV
                     HVCVPHKTTARIQEVHLLALHCMCDGIDFQLFGTKGR"
     gene            2259..>2617
                     /locus_tag="VGO02_09205"
     CDS             2259..>2617
                     /locus_tag="VGO02_09205"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEV7477897.1"
                     /translation="MTRLAWLLALLAALWLAGCAPVLVGAAAGGAFSMSEDRRSSGAQ
                     LDDQSIEWRSSSRIGERFGSKAHINVTSYNRAALLTGEVPDERMRADADALVRSVPSV
                     HGTTNEVVVAEPTSLGSR"
BASE COUNT          383 a          886 c          958 g          390 t
ORIGIN      
        1 cttgagagtg agccggtcca tccgccccga ttttacttgg cgcgcgcgag cgccttgcga
       61 taaagctcgt tgcggggggc gccggtgatg cgggcggcga gcttcgctgc ctcgctgggc
      121 gagacagctt cgagcagcgc cgcgagcacg cggtcggcat cgatggccgg gggcgcctcg
      181 ccgccgggag ccagcaccag cacgaactcg ccctgctggc ggtgcggctg ggcggcgagc
      241 cagcctgccg cctggccaag ggaaagccgc gccacttcct cgaatttctt ggtgagctcg
      301 cgggcgatca gcacctcgcg ctgctcgccg aagcgcgtgc gcagatcctc cagcgtcgcg
      361 gcgatgcggt gcggcgcctc gtacagaacg accggccagg gcagctcgag cgcctgcagc
      421 gccttgcggc gcgcagtggc cgccgcgggc aggaagccga cgaacagaaa cctgtcagtg
      481 gcaaagccac tgactgacag cgcggcggcg gccgcgctcg gcccgggcag cgggcttacg
      541 cggatgcctg cgcggtgcgc ctgctccacc agcagcgcac cgggatcgga cagcgcaggc
      601 gtgcccgcgt cgctcaccag ggcgatgttc ttgccctcga gaagaagggc gatcagcttg
      661 ccggtcgcgg cgcgctcgtt gtgctcgtgc agcgcgacgc tgcgcgcctc tatggcgtat
      721 cgcgccagga gcgtacgggt ggtgcgggtg tcctcgcagg cgatcacctg cgccgagcgc
      781 agcgcctcga tggcgcgcgg gctggcgtcg gcgaggttgc cgatcggagt ggcgacgacg
      841 tacagggtcc cggcaggcat gggttttgga gtcaggggtc aaccaccgcg gcggtgtctt
      901 cgttaagact gccatgaact ggtcgggcgc gcgagccgag gatctctgcg cccaggtcct
      961 gcaaggggcc gggctgcggc tggtggcgcg caactggcgc tgccgcctcg gcgagatcga
     1021 cctgatcgcc gatgagggcg gtaccctggt gttcgccgag gtgcgcatgc gcagcggcgg
     1081 cggcttcggc ggggccgcgg aaagcgtcac cgcggcgaag cgcgggcgcc tgctggcggc
     1141 ggcgcggctc tacctgtcgc gccgtccgga ggcgccgacg cgcttcgatg tgcttctggt
     1201 cgacggcaca gcgggccggg tggagtggat ccgggatgcg ttcggggagt agcgggttcg
     1261 cggttctcgg attgatatgt atccaagcat gggcgcagga gccatcggta cgcctcctcg
     1321 tgcagagttc gccgctcgcc gggtttcgct accacgccgc cgccgaggta tggcaggagc
     1381 tgcgcatcgg cgacctgctc gagctgcagc gcgagccgga caatccgcac gatgcgaacg
     1441 cagtgagcgt ttcctggcgc gggcgcaagc tcggctacct gccgcgccgc gaaaacgccg
     1501 ccgcttcctg gagcctggat cgcggccagc ggctgagcgc gcgcatcagc aggctggcgc
     1561 agcatccgaa cccggcgcgc cgcatcgaag tcgaggtcta tgcggagtga cggtgtaaaa
     1621 tcccccgcca tggcaatcgc gcagcagaac gcggcggaac agggtgcggt gcttgcggcg
     1681 cgcgtggccg cgcagttcgc cgagagcgcg cggctcaagc tcgaagccgc gcagtcgctg
     1741 gtcgggccgc tggtgcgggc cgggaccctc ctcgccgaag cgctgcgcca gggcggcaag
     1801 gtgcttgcct gcggcaacgg cggctccgcg gctgacgcgc agcacttcgc cgccgagctg
     1861 gtcaaccgct tcgaaatgga acgcccgccg ctcgccggga tagcgttgac caccgacacc
     1921 tccacgctca cctcgatcgc caacgactac gcttacctgc aggtattttc caagcaggtc
     1981 cgcgcgctcg gccggcgcgg cgacgtgctg ctggcgattt cgaccagcgg caactccggc
     2041 aatgtggtcg aggcgatcag cgccgcgcac gagctcggct tgcgagtgat cgccctcacc
     2101 ggcaacggcg gcggcaagat ggcagccatg ctcggcccag acgacgtgca cgtgtgcgtg
     2161 ccgcacaaga ccaccgcgcg catccaggaa gtgcacctgc tcgccttgca ctgcatgtgc
     2221 gacggcatcg atttccagct gttcggaaca aagggaagat gacaagactc gcctggctgc
     2281 tcgccttgct tgccgcgctc tggcttgccg gatgcgcgcc cgtgctggta ggggctgccg
     2341 ccggcggcgc gttctcgatg tcggaggatc gccgcagctc gggcgcgcag ctcgacgatc
     2401 agagcatcga gtggcgctcg tccagccgca tcggcgagcg cttcggctcg aaggcccaca
     2461 tcaacgtgac ttcgtacaac cgcgccgcgc tgctcaccgg cgaggtgccc gacgagcgca
     2521 tgcgcgccga cgccgacgcc ctggtgcgct cggtgccgag cgtgcacggc accaccaacg
     2581 aggtggtggt ggccgagccc acttcgctcg gcagccg
//
LOCUS       DASYJA010000421         2634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_244617, whole genome shotgun sequence.
ACCESSION   DASYJA010000421 DASYJA010000000
VERSION     DASYJA010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244617"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VGO02_09210"
     CDS             <1..247
                     /locus_tag="VGO02_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477898.1"
                     /translation="PPVITGDAMDRKDKVEGEGSYTGTKDYNKRTQKFVDSGKVEQAA
                     NDAAPKSEEEAREMQEAERTGKQHIKDEDPAVRGKKP"
     gene            complement(249..839)
                     /gene="msrA"
                     /locus_tag="VGO02_09215"
     CDS             complement(249..839)
                     /gene="msrA"
                     /locus_tag="VGO02_09215"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEV7477899.1"
                     /translation="MKRLVVLLALALPAAAQAQQTEKAAFAMGCFWCAEEAFEKVPGV
                     VQVVSGYTGGKVKNPSYEQVSSGRTGHTEAVQVTFDPSKVSYDKLLDTFWLNHDPTVT
                     DRQFCDAGSQYRPEIFYHSEEQKRLAEASKAKYEKAKPFRQPLLTPITPASEFYPAED
                     YHQDYYKKNPVQYRFYVTGCGRYSRLDQLWGALRKH"
     gene            complement(836..1318)
                     /locus_tag="VGO02_09220"
     CDS             complement(836..1318)
                     /locus_tag="VGO02_09220"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEV7477900.1"
                     /translation="MVRGDLLEEPVEAIVNAANGRLAHGGGVAGIISRAAGPALQAES
                     DALVAARGSFPTGSAIVTTAGALPFKGVIHAVGPRYGEGDEERKLFEALVNSFRLALE
                     NGWNSLAFPAVSSGIFAVPLEICARAYLRAARVSPLRNVRLCLRDQPVIDAVLKEMKA
                     "
     gene            complement(1339..1548)
                     /locus_tag="VGO02_09225"
     CDS             complement(1339..1548)
                     /locus_tag="VGO02_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HEV7477901.1"
                     /translation="MARYGRKSRQKVKRAMHERKRGTLRSGRSGKKVKSRKQAIAIGL
                     SEARRAGAKVPRRKGRKRRSSRSKK"
     gene            complement(1569..2420)
                     /locus_tag="VGO02_09230"
     CDS             complement(1569..2420)
                     /locus_tag="VGO02_09230"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV7477902.1"
                     /translation="MRNWMYAAALLAAPALAQLPLPTKDEPAKKESAKKETAKSQPAA
                     QGTTIAKVNGVAVPRARQDLLMQQQSARGMPDNEQTRALLRDELINREVLFQEAQKSG
                     VGKKPEVQAQLEMTRQEVLVSAYVRDWVRTHPVTDADVQKEYERAKTQAGDKEYRARH
                     ILVETEDQAKGLIEELKKGGKFEDLATKNSKDPGSAQRGGDLDWNVPGTYDKMFADAL
                     LKLEKGKYTDAPVHTRFGYHIIMLEDVRPVKFPALNEVRQRISQQITQARIEELVKSL
                     RAKAKVE"
     gene            complement(2417..>2634)
                     /locus_tag="VGO02_09235"
     CDS             complement(2417..>2634)
                     /locus_tag="VGO02_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEV7477903.1"
                     /translation="VDESEQHRGHAGYREGGGTHWRLAIVSRRFSGRSTLERHRMIYA
                     ALDNLMQHPIHALAITARSPEETKGKQ"
BASE COUNT          377 a          897 c          868 g          492 t
ORIGIN      
        1 tcctccggtc atcacaggag acgccatgga tcgcaaggac aaggtcgaag gcgaaggcag
       61 ctacaccggc accaaggact acaacaagcg cacgcagaag tttgtcgact ccggcaaggt
      121 cgagcaggcg gcgaacgacg ccgcgccgaa gtccgaggaa gaggcacgcg agatgcagga
      181 ggccgagcgc accggcaagc agcacatcaa ggatgaagac ccggcggtac gcggaaagaa
      241 accctaggtc agtgtttccg tagcgcgccc cagagctgat ccaggcgcga gtagcgcccg
      301 cacccggtga cgtagaagcg gtattgcacc gggttcttct tgtagtaatc ctggtggtag
      361 tcctcggccg gatagaactc gctcgccggg gtgatcggcg tgagaagcgg ctggcggaac
      421 ggcttggcct tctcgtactt ggccttcgac gcttccgcga ggcgcttctg ctcctcggag
      481 tggtagaaga tctccgggcg gtattgcgag cccgcgtcgc agaactgccg gtcggtcacc
      541 gtcgggtcgt ggttgagcca gaaggtgtcg aggagcttgt cgtagctcac cttcgagggg
      601 tcgaaggtca cctgcaccgc ctcggtgtgg ccggtgcggc cgctcgagac ctgctcgtag
      661 ctggggttct tgaccttgcc gccggtgtag cccgaaacca cctggaccac gccgggcacc
      721 ttctcgaaag cctcctcggc gcaccagaag cagcccatgg cgaacgccgc cttttcggtc
      781 tgctgcgctt gcgccgcggc cgggagcgcg agcgccagaa gaacgacgag ccgtttcatg
      841 ccttcatctc cttgagaacc gcatcgatga ccggttggtc gcgcaagcat agccgcacat
      901 tacgcagggg agagacgcgc gcggcccgga ggtaggcccg ggcgcagatc tccagcggca
      961 ccgcgaagat gccgctcgac accgcgggga aggccaggga attccagccg ttctccagag
     1021 ccagcctgaa ggaatttacc aaggcctcaa agagcttgcg ctcctcgtcg ccttcgccgt
     1081 agcgcggtcc gacggcgtgg atcacgccct tgaagggaag cgctccggcg gtggtgacga
     1141 tcgccgagcc ggtcgggaaa gagccgcgcg cagcgacgag cgcgtcggac tcggcctgca
     1201 gggccggccc ggcggcgcgc gagatgatcc cggcgacgcc tccgccgtgc gcgagacgcc
     1261 cgttggcggc gttgacgatc gcctcgaccg gctcctcgag gagatcgccc cgaaccacct
     1321 ggaacaggcg ccccggcgct acttcttgct gcgcgaggac ctgcgctttc tgccctttct
     1381 gcgcggcacc ttggcgcccg cgcgccgcgc ctccgacaag ccgatggcga tcgcttgctt
     1441 gcggctcttc accttcttgc cggagcggcc gctcctcagc gtgccgcgtt tcctttcgtg
     1501 catcgcccgc ttcaccttct gccgcgactt cctgccgtac ctcgccatgg ttcctcctgt
     1561 gaggtccgct actccacctt cgccttcgcg cgcagcgatt tcaccagctc ctcgatccgg
     1621 gcctgggtga tctgctggct gatgcgctgg cgcacttcgt tcagcgccgg gaacttcacc
     1681 gggcgtacgt cctccagcat gatgatgtgg tagccgaagc gggtgtgcac cggcgcgtcg
     1741 gtgtacttgc ccttctccag cttcagcaac gcgtccgcga acatcttgtc gtaggtcccc
     1801 ggcacgttcc agtcgaggtc gccgccgcgc tgcgcgctgc ccgggtcctt cgagttcttc
     1861 gtcgccaggt cctcgaactt gccgcccttc ttcagctctt cgatcaggcc cttcgcctgg
     1921 tcctcggtct ccacgaggat gtggcgggcg cggtattcct tgtcgccggc ctgcgtcttg
     1981 gcgcgctcgt actccttctg cacgtcggcg tcggtcaccg ggtgggtgcg cacccaatcc
     2041 cggacatagg cgctcaccag cacctcctgg cgcgtcatct cgagctgggc ctgcacctcg
     2101 ggcttcttgc cgacgccgct cttctgcgct tcctggaaca gcacctcgcg gttgatgagc
     2161 tcgtcgcgca ggagcgcgcg cgtctgctcg ttgtcgggca tgccgcgggc gctctgctgc
     2221 tgcatcagga ggtcctggcg cgcgcgcggc acggccacgc cgttgacctt ggcgatcgtg
     2281 gtgccctggg ccgcgggctg tgacttggcc gtctccttct tcgcggattc cttcttggcg
     2341 ggctcgtcct tcgtcggcag cggcagctgc gccagcgccg gggcggccag cagggccgcg
     2401 gcgtacatcc agtttctcat tgctttcctt tcgtctcttc cgggctgcgc gccgtgatcg
     2461 cgagcgcgtg gatggggtgc tgcatcagat tgtcgagggc ggcgtagatc atgcggtgcc
     2521 gctcgagcgt gctgcgcccg ctgaagcgcc gcgacacgat ggccaggcgc cagtgcgtgc
     2581 cgccgccttc ccggtagccg gcatggccgc ggtgctgctc gctctcgtcg acga
//
LOCUS       DASYJA010000422         3351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_153437, whole genome shotgun sequence.
ACCESSION   DASYJA010000422 DASYJA010000000
VERSION     DASYJA010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153437"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2309
                     /locus_tag="VGO02_09240"
     CDS             <1..2309
                     /locus_tag="VGO02_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HEV7477904.1"
                     /translation="VEIMQQTPEIPESAQWAIFLRNHDELTLEMVTSRERDYMYRQYA
                     ADTQARINLGIRRRLAPLMENDSDRIRLMNSLLLSMPGSPIIYYGDEIGMGDNIYLGD
                     RNGVRTPMQWSPDRNAGFSRADPQKLYLPPIMDPVYGYDAVNVESQMRDPSSQLNWMK
                     RMLATRATSKAFGRGKLTFLRAGNRKILSFLRETKEEAILCVANLARTAQPVELELKR
                     YKGRVPVEMLGRTAFPPIGELPYMLTLPAHAFYWFRLATDATPPDWHRDMLVREEAPV
                     MVLFDGWTSFFRDQVVPWRIGMAEKLRAELEGQVLPRYVEAQRWYAAKGEPVKQARIV
                     DHAVWQEGPVSWLLAFLGVSSDKYFVPLALAWEDEEEQVRALAAGTVARIRQQANVGL
                     IGDALADEAFCRHVVKAIGGSRTLPTAAGKVHFAPTRTFGALATEDLAALSMGPVKTA
                     STNTSVQIGERFFLKCYRRLRSGMHPELEIGRFLTEVAQFPHCVPLAGSVEYVAKEGG
                     EAGSEPATLMLLQAYLPNQGDGWQYTLGFLQRYLESAGTQEGLGAYLSLIQTLGVRTA
                     ELHRAFTTRSGDPAFEPEPFTAQDAQEWKARVRQEAESTFALLKEDQFDDARKGRLLA
                     RIASCAAPKKPALKTRHHGDYHLGQVLLANNDFVIIDFEGEPGRTHAESRRKRSPLRD
                     VAGMLRSFSYARWSASDKDNPVLDDWEAQTRRAFLGAYGVDDDQRGLLALAEIEKLLY
                     ELRYELANRPDWIHVPLRGALGVLGEKA"
     gene            2306..2974
                     /locus_tag="VGO02_09245"
     CDS             2306..2974
                     /locus_tag="VGO02_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477905.1"
                     /translation="MNGIDVYLEPLRVFLHQAADFLPRLGLALVVLIAGWILAKMARF
                     AIVRTLRAINFNILTERAGVDGFLRDGGIQADTTEILALLTYWLVILAALVIGFNGLG
                     LTYITDLLGRVLLFVPKVMVALLILAFGAYFARFVGNAVCAYFRNVHLQDADLLGKLA
                     QYAILTFVVLIALDQVNVGGDIVRQTFLIVLAGVVFALALAFGLGGKDWAAEMLERWW
                     PRRK"
     gene            2956..>3351
                     /locus_tag="VGO02_09250"
     CDS             2956..>3351
                     /locus_tag="VGO02_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006767354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477906.1"
                     /translation="MAETQIGTRPVQQLSSFGAQLTPEGVRFRLWAPAAKSVDVVFIE
                     TTRPMHPAGDGWFEHLEPEAGAGTLYQYQIDSKLLVPDPASRFQPRDVDGPSEVIDPA
                     FAWEEWKGRPWEEAVIYELHVGTFTPAGTF"
BASE COUNT          561 a         1157 c         1100 g          533 t
ORIGIN      
        1 tggtcgagat catgcagcag acgccggaga tcccggaaag cgcgcagtgg gcgatcttcc
       61 tgcgcaacca cgacgagctg accctcgaga tggtgaccag ccgcgagcgc gactacatgt
      121 accgccagta cgccgccgac acgcaggcgc gcatcaacct cggcatccgg cggcgcctcg
      181 cgccgctgat ggagaacgac tccgaccgca tccggctgat gaacagcttg ctgctgtcga
      241 tgccgggctc gccgatcatc tactacggcg acgagatcgg catgggcgac aacatctatc
      301 tcggcgaccg caacggcgtg cgcacgccca tgcagtggag ccccgaccgc aacgccggct
      361 tctcgcgcgc cgacccgcag aagctgtatc tgccgccgat catggacccg gtctacggct
      421 acgacgcggt caacgtcgag tcgcagatgc gcgacccgag ctcgcagctc aactggatga
      481 agcgcatgct cgccacgcgc gccaccagca aggcgttcgg ccgcggcaaa ttgacgttcc
      541 tgcgggccgg caacagaaag atcttgagct tcctaagaga aacaaaagag gaagccatcc
      601 tctgcgtggc caacctcgcc cgcaccgcgc agccggtcga gctcgagctc aagcgctaca
      661 aggggcgcgt gccggtcgag atgctcggcc gcaccgcctt cccgccgatc ggcgagctgc
      721 cctacatgct gacgctgccg gcgcacgctt tctactggtt ccgcctcgcc accgacgcca
      781 cgccgcccga ctggcaccgc gacatgctgg tgcgcgagga ggcgccggtg atggtgctgt
      841 tcgacggctg gaccagcttc ttccgcgacc aggtggtgcc gtggcgcatc ggcatggccg
      901 agaagctgcg cgccgagctc gagggccagg tcctgccgcg ctacgtcgaa gcgcagcgct
      961 ggtacgcggc gaagggcgag ccggtcaagc aggcgcgcat cgtcgaccac gcggtgtggc
     1021 aggaagggcc ggtcagctgg ctgctcgcgt ttctcggagt ctcctccgac aagtactttg
     1081 ttccgctggc gctcgcctgg gaggacgagg aggagcaggt gagggcgctc gccgccggca
     1141 cggtggcgcg catccgccag caggcgaacg tcggcctgat cggcgacgcg ctggctgacg
     1201 aggctttctg ccgccacgtg gtgaaggcga tcggcggctc ccgcactttg ccgactgccg
     1261 ccggcaaagt gcactttgcg ccgacccgca ccttcggcgc gctcgccacc gaggacctgg
     1321 cggcgctcag catgggtccg gtgaagacgg cgagcaccaa cacttcggtg cagatcggcg
     1381 agcgcttctt cctcaagtgc taccggcgcc tgcgctcggg gatgcatccc gagctcgaga
     1441 tcgggcgctt cctcaccgag gtggcgcagt tcccgcactg cgtgccgctg gcgggctcgg
     1501 tcgagtatgt cgcgaaggag ggcggcgagg caggctccga acccgccacc ttgatgctgc
     1561 tgcaggccta tcttcccaac cagggcgacg gctggcagta cacgctcggt ttcctgcagc
     1621 gctatctgga gagcgcgggc acgcaggaag ggctcggcgc ctacctcagc ctgatccaga
     1681 ccctcggcgt gcgcaccgcc gagctgcacc gcgccttcac cacgcgctcg ggcgatccgg
     1741 cgttcgagcc ggagccattc accgcgcagg acgcgcaaga gtggaaggcg cgcgtcaggc
     1801 aggaggccga gtcgaccttc gcgctgctga aggaggatca gttcgacgac gcgaggaaag
     1861 gccgccttct cgcgagaatc gcctcgtgcg ccgcgccgaa aaagcccgcg ctcaagacac
     1921 ggcaccatgg cgactaccac ctcggacagg tgctgctcgc caacaacgac ttcgtcatca
     1981 tcgacttcga gggcgagcct ggccgcacgc acgccgagtc ccggcgcaag cgctcgccgc
     2041 tgcgcgacgt cgccgggatg ctgcgctcct tcagctatgc gcgctggagc gcatccgaca
     2101 aagacaaccc ggtgctcgac gattgggagg cgcagacgcg ccgcgcgttc ctcggcgcct
     2161 acggcgtcga cgacgaccag cgcgggctgc tcgccctggc cgagatcgag aagctgctct
     2221 acgagctgcg ctacgagctc gccaaccgtc ccgactggat ccacgtgccg ctgcgcggcg
     2281 cgctcggcgt cctaggagaa aaagcatgaa cggcatagac gtatacctgg agcccttgcg
     2341 cgtgttcctg caccaggcgg ccgacttcct gccgcgcctg ggcctcgcgc tggtggtgct
     2401 gatcgccggc tggatcctcg ccaagatggc gcgcttcgcc atcgtgcgca ccctgcgcgc
     2461 gatcaacttc aacatcctca ccgagcgcgc cggagtcgac ggcttcctgc gcgacggcgg
     2521 catccaggcc gacaccaccg agatcctggc gctgctcacc tactggctgg tgatcctcgc
     2581 tgcgctggtg atcggcttca acggcctggg cctcacgtac atcaccgacc tcctcggccg
     2641 ggtgctgctg ttcgtgccca aggtgatggt ggcgctgctg atcctcgcct tcggcgccta
     2701 cttcgcgcgc ttcgtcggca acgcggtctg cgcctatttc cgcaacgtgc acctgcagga
     2761 cgcggatctg ctgggcaagc tcgcgcaata cgcgatcctc acctttgtgg tgctgatcgc
     2821 gctcgaccag gtgaacgtcg gcggcgacat cgtgcgccag accttcctca tcgtgctggc
     2881 cggcgtggta ttcgcgctgg cgctcgcctt cgggctgggc ggcaaggact gggcggcgga
     2941 aatgctggag cgctggtggc cgagacgcaa atagggacac gccccgtcca gcagctctcc
     3001 tccttcggcg cgcagctcac gcccgaaggc gtgcgctttc ggctgtgggc gcccgccgcg
     3061 aaatccgtag acgttgtttt catcgaaaca acaagaccaa tgcatccggc cggcgacggg
     3121 tggttcgagc atctcgagcc ggaggccgga gcaggcacgc tgtaccaata ccagatcgac
     3181 agcaagctgc tggtgcccga tcccgcttcg cgctttcagc cgcgggacgt cgacggtccc
     3241 agcgaggtca tcgatccggc cttcgcatgg gaagagtgga aaggccggcc gtgggaagaa
     3301 gctgtcatct acgagctgca tgtaggtact ttcacgcccg caggaacctt t
//
LOCUS       DASYJA010000423         4306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1334181, whole genome shotgun sequence.
ACCESSION   DASYJA010000423 DASYJA010000000
VERSION     DASYJA010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334181"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VGO02_09255"
     CDS             complement(<1..625)
                     /locus_tag="VGO02_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEV7477907.1"
                     /translation="MLNATLSELSAALQAKKISAVELTQQYLGRIGRFGRALNAFITV
                     DESRALADARRADERRARGESGPLLGIPVAHKDIFCTRGMLTTCGSKMLANFKSPYDA
                     HVIERFDAAGAVLLGKCNMDEYAMGSSSETSHFGPVRNPWDQRAVPGGSSGGSAAAVA
                     ARLAPAATGTDTGGSIRQPASFSGVSGLKPTYGTVSRYGIVAFASSLD"
     gene            complement(626..913)
                     /gene="gatC"
                     /locus_tag="VGO02_09260"
     CDS             complement(626..913)
                     /gene="gatC"
                     /locus_tag="VGO02_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004574467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEV7477908.1"
                     /translation="MSLSDDQVRRIARLARLDLAPQEVAPMAERLNRLLGLIEQMRAV
                     DTAGIDPMAHPLDASQRLRPDEVTEQDRREAYQSVAPQKQDGLYLVPKVIE"
     gene            1092..2135
                     /locus_tag="VGO02_09265"
     CDS             1092..2135
                     /locus_tag="VGO02_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEV7477909.1"
                     /translation="MFGFLRSYFSNDLAIDLGTANTLIYVRGKGIVLDEPSVVAIRQE
                     AGPNGKKTIQAVGKEAKQMLGKTPGNIIAIRPMKDGVIADFTVTEQMLKQFIKKVHDN
                     KLFSPSPRIIICVPCGSTQVERRAIRESAIGAGAGQVFLIEEPMAAAIGADLPVADAT
                     GSMVVDIGGGTTEVGVISLGGMVYSGSVRVGGDKFDEAIINYIRRNYGMLIGETTAEL
                     IKKEIGSAFPGSEVREMEVKGRNLAEGIPRSFTISSNEILEALTEPLNQIVSAVKSAL
                     EQTPPELGADIAEKGMVLTGGGALLRDLDRLLMEETGLPVIVADDPLTCVVRGSGKAL
                     EQMEHFGPIFTND"
     gene            2148..3077
                     /gene="mreC"
                     /locus_tag="VGO02_09270"
     CDS             2148..3077
                     /gene="mreC"
                     /locus_tag="VGO02_09270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEV7477910.1"
                     /translation="MEHSPPPFFKRGPAPLVRLFFFASLSLALLVLDARFQYGQGVRS
                     LLALVAYPLQVIATAPIDLAQGIGGYFSNQSQLIEDNAELRSKALTYARDAQRYQAAE
                     AEAAQLRRLIGATERLGVRAQPTELLYAGRDPYSQKVFIDRGATHGVRPGSPVADEAG
                     IVGQVTRVHPLLSEVTLITDRDQATPVQVVRNGLRAVAFGGGASGMLELRFMAAIAEI
                     QEGDRLVTSGIDGTYPAGLPVATVVKVERDPEQGFAHVVCKPAAGVDRGRYLLVLADD
                     AVRPPRPEEAQQGKDRREKSRRTKSRESNDGSR"
     gene            3064..3573
                     /gene="mreD"
                     /locus_tag="VGO02_09275"
     CDS             3064..3573
                     /gene="mreD"
                     /locus_tag="VGO02_09275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEV7477911.1"
                     /translation="MGPDSSRHILMPARLSTVALSFAIALVLDFLPWKDLRLVPEFAA
                     LVLTFWCVRQPRLVGLGIGWSIGLISDAGNGVLLGQHALAYSVLAFLALWLSRRILWF
                     GAGLQALHVALMLLACEGVTLLVRLAAGDQSPGWPIIIGPLAGAALWPMVSWLLLLPQ
                     RRLEREQTI"
     gene            3576..>4306
                     /locus_tag="VGO02_09280"
     CDS             3576..>4306
                     /locus_tag="VGO02_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEV7477912.1"
                     /translation="MSFAELRNYERDLHQFHLRLAVAGIAVFVAFGLLAARFIFLQVV
                     QHDVYASKAEDNRISIVPIPPNRGLIVDRNGVVIARNYSGYTLDIFPRRVKSVEGTIN
                     ELAELIDITPRDRARFKKVMLETRNPESVPIRTRLSDEEVARFAVNRYRYEGVEIKAR
                     LFRQYPFGDVASHAIGYMGRVNKDDQARLEEEGIDANYRGTDFTGRTGVEGSYESELH
                     GTTGFEQVEIDAAGRGIRTLSRSAP"
BASE COUNT          710 a         1461 c         1454 g          681 t
ORIGIN      
        1 ggtcgagcga cgaagcgaag gcgacgatgc cgtaacgcga gaccgtgccg taggtcggct
       61 tgagccccga tacgccggag aacgaggccg gctggcggat cgagccgccg gtgtcggtcc
      121 cggtggcggc gggcgccagc ctcgccgcca cggcggccgc ggatccaccg gaagagccgc
      181 cgggaacggc gcgctggtcc caggggttgc gcaccgggcc gaaatgcgag gtctcgctcg
      241 aggaacccat cgcgtactcg tccatgttgc acttgccgag cagcacggcg ccggcggcgt
      301 cgaagcgctc gatgacgtgc gcgtcgtagg ggctcttgaa gttggccagc atcttcgagc
      361 cgcaggtggt gagcatcccg cgggtgcaga agatgtcctt gtgcgcgacc gggataccaa
      421 ggagcggccc gctctcgcct cttgcccggc gctcgtcggc ccgcctggca tcggcgagcg
      481 cgcgcgactc gtcgacggtg atgaaggcgt tcagggcgcg gccgaagcgg ccgatacgtc
      541 ccaggtactg ctgagtcagc tcgaccgcgg agatcttctt cgcctgaagg gctgccgaga
      601 gctccgaaag cgtcgcgttc agcacctatt cgatcacttt cggcacgaga tagaggccat
      661 cctgcttctg cggagcgacg gactggtagg cctcccgccg gtcctgctcg gttacctcat
      721 ccggccgtaa ccgctgcgat gcgtccaagg ggtgcgccat ggggtcgatg ccggcggtgt
      781 ccaccgccct catctgctcg atcaggccga gcagccggtt gagccgctcg gccatcggcg
      841 ccacctcctg cggcgcgagg tcgaggcgcg ccaggcgggc gatgcggcgg acttggtcgt
      901 cggaaagcga catgaaaatg cggtgggacc tacgggccgg ctcccgcgac gcgggacttt
      961 tcccttcccc tcgggcgttg ttagcgtatc atatccgccg ttcaggtccg tggcttaggc
     1021 cctgaatcta aggcgaatta gtagaaataa cggaaagacg gtcaaagcgg aaaggcgctc
     1081 acagcttgcc catgttcgga ttcctgcgaa gctacttctc gaacgatctg gcgatcgacc
     1141 tcgggactgc aaatactctc atctacgtac gcggcaaagg catcgtgctc gacgagcctt
     1201 cggtcgtggc catccggcaa gaagccggcc cgaacggcaa gaagacgatt caagccgtcg
     1261 gcaaggaggc gaagcagatg ctcggcaaga cgccgggcaa catcatcgcc atccgcccga
     1321 tgaaggacgg cgtcatcgcc gacttcaccg tcaccgagca gatgctcaag cagttcatca
     1381 agaaagtgca cgacaacaag ctgttctcgc ccagcccgcg catcatcatc tgcgtgccgt
     1441 gcggctccac tcaagtggag cggcgcgcca tacgggaaag tgccatcggc gcgggcgccg
     1501 gccaggtctt cttgattgaa gaaccaatgg ctgcagccat tggtgccgac ctgcccgttg
     1561 ctgatgcgac gggctccatg gtcgtcgata tcggcggcgg caccaccgag gtgggcgtga
     1621 tctccctcgg cggcatggtg tactcgggct cggtgcgcgt cggcggcgac aagttcgacg
     1681 aagcgatcat caactacatc cgccgcaact acggcatgct gatcggcgag accaccgcgg
     1741 agctgatcaa gaaagagatc ggctcggcct tccccggctc cgaagtgcgc gagatggaag
     1801 tgaagggccg caacctcgcc gagggcattc cgcgcagctt caccatctcc tccaacgaga
     1861 tcctcgaggc gctgaccgag ccgctcaacc agatcgtctc ggcggtcaag tcggcgctcg
     1921 agcagacgcc gcccgagctc ggcgcggaca tcgccgagaa gggcatggtg ctcaccggcg
     1981 gcggcgcgct gctgcgcgac ctcgaccggc tgctgatgga ggaaacgggc ctgccggtga
     2041 tcgtggccga cgatcccctg acctgcgtgg tacgcggttc gggcaaggcg ctcgagcaga
     2101 tggagcactt cggcccgatc ttcaccaacg actgagtgta gggggcgatg gagcatagcc
     2161 ccccgccgtt cttcaaacgg ggaccggcgc ctctcgtcag gctgttcttt ttcgcctcgc
     2221 tttcgctggc actgctggtc ctcgacgcgc gtttccaata cggccagggc gtgcgctcgc
     2281 tgctggcgct cgtcgcctac ccgctgcagg tgatcgccac ggcgccgatc gacctcgcgc
     2341 agggaatcgg cggctacttc tccaaccagt cgcagctgat cgaggacaac gcggagctgc
     2401 gctccaaggc gctgacctac gcgcgcgacg cgcagcgcta ccaggcggcc gaagccgaag
     2461 ccgcgcagct gcggcgcctc atcggcgcca ccgagcggct gggagtacgc gcgcagccga
     2521 ccgagctgct ctatgccggg cgcgacccgt attcgcagaa ggtgttcatc gatcgcggcg
     2581 cgacgcacgg cgtgcgcccg ggcagcccgg tggccgacga agccggcatc gtcggccagg
     2641 tgacgcgggt gcatccgctg ctctcggaag tgacgctgat caccgaccgc gaccaggcga
     2701 ctccggtgca ggtcgtgagg aacggcctgc gcgcggtggc cttcggcggc ggcgcctcgg
     2761 gcatgctcga gctgcgcttc atggcggcga tcgccgagat ccaggagggc gaccgcctgg
     2821 tcacctccgg catcgacggc acctacccgg cggggctgcc ggtcgccacg gtggtgaaag
     2881 tcgagcgcga ccccgagcag ggattcgccc acgtcgtgtg caagccggcc gccggcgtcg
     2941 accgcggccg ctacctgctg gtgctcgccg acgacgcggt gcgcccgccg cggcccgaag
     3001 aggcgcagca aggcaaggac cgccgcgaga agtcgcggcg cacgaagtcg agagagtcga
     3061 acgatgggtc ccgatagctc gcggcacatc ctgatgccgg cgcggctcag caccgtcgcg
     3121 ctgtcgttcg ccatcgcgct ggtgctcgac ttcctgccgt ggaaggacct gcgcctggtg
     3181 ccggagttcg ccgcgctggt gcttactttc tggtgcgtgc ggcagccgcg cctggtcggc
     3241 ctgggcatcg gctggtccat cggcctgatc agcgacgccg gcaacggcgt gctgctcggg
     3301 cagcacgcgc tcgcctattc ggtgctcgct ttcctcgccc tgtggctctc gcggcgcatc
     3361 ctgtggttcg gcgcggggtt gcaggccttg cacgtcgcgc tcatgctgct ggcctgcgag
     3421 ggcgtcacgc tgctggtgcg cctggcggcc ggcgaccagt cacccggctg gccgatcatc
     3481 atcgggccgc tggccggcgc cgcgctctgg ccgatggtga gctggctgct gctcctgccg
     3541 cagcggcgcc tggaacgcga gcagacgatc tagccatgag cttcgccgag ctgcgcaact
     3601 acgaacgcga cctgcatcag ttccacctgc ggctggcggt cgccggcatc gccgtgttcg
     3661 tcgccttcgg cctgctcgcc gcgcgcttca tcttcctgca ggtggtgcag cacgacgtct
     3721 acgcctccaa ggcggaagac aaccgcatct cgatcgtccc gatcccgccc aaccgcgggc
     3781 tgatcgtcga ccgcaacggc gttgtcatcg cgcgcaacta ctcgggctat acgctggaca
     3841 tcttcccgcg ccgcgtcaaa agcgtcgagg gaacgatcaa cgagctcgcc gagctgatcg
     3901 acatcacgcc gcgcgaccgc gcccgcttca agaaggtgat gctcgagacg cgcaacccgg
     3961 aaagcgtgcc gatccgcacc cgtctcagcg acgaggaggt ggcgcgcttc gcggtgaacc
     4021 gctaccgcta cgaaggcgtc gagatcaagg cgcgtctttt ccgccagtac cccttcggcg
     4081 acgtcgcctc gcacgccatc ggctacatgg gacgtgtcaa caaggacgac caggcgcgcc
     4141 tcgaggagga aggcatcgac gccaactacc gcggcaccga cttcaccggc cgcaccggcg
     4201 tggagggaag ctacgagagc gagctgcacg gcaccaccgg cttcgagcag gtagagatcg
     4261 acgccgccgg acgcggcatc cgcacgctgt cgcgcagcgc cccgca
//
LOCUS       DASYJA010000424         8864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3242153, whole genome shotgun sequence.
ACCESSION   DASYJA010000424 DASYJA010000000
VERSION     DASYJA010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8864
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3242153"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VGO02_09285"
     CDS             complement(<1..350)
                     /locus_tag="VGO02_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEV7477913.1"
                     /translation="MTRRGYEGVAVAVPVTVPYVRYSIRSAHWWLARALDSLLKQSKL
                     SKNEIDGLTISSFTLQPDTAIGLTQHLGLAPRWLDHIPLGGASGVVALRRAARAVQSG
                     DASVVACLAGDTNHV"
     gene            complement(347..1795)
                     /locus_tag="VGO02_09290"
     CDS             complement(347..1795)
                     /locus_tag="VGO02_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7477914.1"
                     /translation="MESVYAAFEHTARRHGGRPFLHPPGFAYAETLEKVGALAARYRA
                     RGYGAGHRVALKLGNRPEFLLHFLALNSLGASIVPLNPEYRQAELDYVLRHSEACLVV
                     DENFAELPAAPPPGDAAECALLYTSGTTGKPKGCLLTNFYFLNVARRYLAEGGLCEVR
                     EGEERLITPLPLFHMNSLAVSTVAMILTGGCVIQLDRFHPRSWWQEVALSHATIVHYL
                     GVMPAILLGQAPGPYDRAHRVRFGYGANANPKDHASFEERFGFPLIEAWAMTETGGGA
                     LIAASREPRHVGSRCIGFPPDALEVHVEEETGELLVRQKGPDPRKGFFSGYYKDEAAT
                     EEAWHGGWFHTGDAVRLGPDGQLHFVDRRKNIIRRSGENIAALEVEAALAGHPAIQQV
                     AVIAAPDPVRDEEVMACVILTDQKQKNIETAKSIQDWCLERLSYFKAPGYVAFLDELP
                     VTSTNKVQKGKLADFAVNPSNCFDLRDRKKRT"
     gene            2048..2452
                     /locus_tag="VGO02_09295"
     CDS             2048..2452
                     /locus_tag="VGO02_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7477915.1"
                     /translation="MSVSTTNPTKVLVIDDSNTIRRSAEMFLRQAGYEVILAEDGFDA
                     LAKISDHQPQVIFVDIMMPRLDGYQTCALIKQNPVLKATPVIMLSSKDGVFDRARGRL
                     AGSDRYLTKPFTKEALIEAVEQTAKPATLSSS"
     gene            2474..2839
                     /locus_tag="VGO02_09300"
     CDS             2474..2839
                     /locus_tag="VGO02_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7477916.1"
                     /translation="MPIKNILVVDDSPTDRQHLSDILTKSGYKVTTANSAEEALAKVK
                     LSAPDLVLMDVVMPGQNGYQATRQLTKDDATKHIPVIICTTKGQETDKLWGMRQGARD
                     YIVKPVIQSDLLAKIAALG"
     gene            2859..3368
                     /locus_tag="VGO02_09305"
     CDS             2859..3368
                     /locus_tag="VGO02_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEV7477917.1"
                     /translation="MAERLSLKDYQRGLAERLREAGAGRMASKLGMQLGTETWLVDLG
                     DAGEVIPVPAVTPVPLTRPWFKGVTNVRGNLYSVIDFPAFLGVGAVALGEHSRLLLLG
                     ERFRTSAALLIDRSLGLRNPEQLTPRAANGEAPAWLRGEFDDAEGKAWKELDVPQLVR
                     HADFLSVSA"
     gene            3383..4507
                     /locus_tag="VGO02_09310"
     CDS             3383..4507
                     /locus_tag="VGO02_09310"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7477918.1"
                     /translation="MKKIVFATLACAALPAPAQQPGVSADRILLGQAAVFTGPAAQLG
                     IQMRNGIKSYFDFVNEKGGVHGRKLELVSEDDKYEPAVATTASQALIEKHKVFALMGY
                     VGTPTGVAHLKVTTAAKVPLVGMFTGAEVLREPMSRYVFHVRASYYDETDKIVEQVVS
                     TGGKKIAVFYQADAYGEAGRMGTEIALKKRGLQIHSNGTVERNTVAVEKAVETIQASE
                     PDAVVMVGAYTACAEFIRQMKKAGSGAQFYNVSFVGSAALAKALDKEGVGVAISQVVP
                     FPWATGVPVVKEYQALAKKAGFADYNFSAMEGFLVAKVMVEGLRRTGKNLTREGFVDT
                     MEKMQDVDIGGFFVTYSPKSHTGSKFVDLTIIGREGKFLR"
     gene            4520..6619
                     /locus_tag="VGO02_09315"
     CDS             4520..6619
                     /locus_tag="VGO02_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEV7477919.1"
                     /translation="MAFALKLPFFGRRADAEEAEAPAPAAERPKSRGFGTGRKLQILI
                     TLLIVLLAVDGVIVALDARQGTFGTLYIASVGKIRMLSQRLAKAAQQASQGNIEAFKQ
                     LKESREEFASLMKLLTDGGQTAGTSLPPTSAAARPALDALDKEWKKTEKNSGLVVNEQ
                     ANLVALGNAVRSINASNPTLLELADEIAAMSVQAGGSLRQNAITSQLVMLTQRMAKNA
                     NTMLAGDVVDPEVAFLLGKDTNTFRDTLQGLLSGNEGQRITRVGDAEMRGKLSELESG
                     FKEYQRAVSDILGNMQRLVNAKRATRDIFNDSEALLRTSEALNERYEAELGGRQTNFI
                     ALAVVSLLAFVVLLLVGKVYVDDSRIRAEDSEKVNRTNQEAILRLLDEIGNLANGDLT
                     VRARVTEDITGAIADSINYTIDELRRLVSGITEASTQVTAATQEASTVTGQLLQATQK
                     QSTEIQGAGQSVTQMAQQMTDVSVKANDSAKVAETSVSAAQKGTAAVQNAIKGMNDIR
                     EQIQETSKRIKRLGESSQEIGEIVQLISDITEQTNVLALNAAIQAASAGEAGRGFTVV
                     AEEVQRLAERSGEATKHIGAIVKSIQRDTQDAVEAMERSTANVVAGTKTADEADQALR
                     EIGSVSNTLADLIRSISTSTQQQSASATRVAANMKVILGITQLTTEGTKKTAGSAARL
                     TTLADGLRSSVSGFKLA"
     gene            6652..>8864
                     /locus_tag="VGO02_09320"
     CDS             6652..>8864
                     /locus_tag="VGO02_09320"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEV7477920.1"
                     /translation="MANATLLSWINAEVDQALKLVRDNIAKVSGKPEDDAMLRVCPDH
                     LHQVSGALRMVGLAGATRFCEAIEGSFSGLSSQAPRNNGAAIGTIDRAVLALKEFVAD
                     LARGQADVPLRLYPVYKELATLSGKNDASEKELFFPDLTQSAPAHPEAKTLLQADLVP
                     FLQAQRAVFQRGMLGWLRKQPAGLEEMRQALDALYRIAAQLPEPRALWWAALGLVEGM
                     GQSQDADWQTSVKPLCNKIDFQMRDLAAGSSKVQDTLLRDLLYAVAKCKPVTPRIKEI
                     RQLYQLESLFPEAPKAGGGVMEFDIEFLEVALYDLHSRLEALKGAWVQYVSGEQKSAG
                     RFRELVGAFKAKASELGNQHLIKLLDAIGLVAAKLPDPYPRQNQFMVIEMASAFLLVE
                     QVIDHFTTPPDDLAPQIVIMGGWLLDAAKGKSSGEVPAGMRPDLTQQIGAMQLRAQVA
                     KEIIANLQHVEQVLDAYVRDASKRDTLAALPPYLRQIHGALMVLRFDRAAEALAICEK
                     MIAACAEKELADSAEDIDWIAEGLSSLGFFLEPCLHGREPAEQAIQLFFERYGKRSGA
                     AEVAPATPAAAAAEGADLTIKLDFAAMAADVTQKMSAAADAPRPSVAPPVRRAAVDAE
                     LLGIFLDEAGEVLQTINDTVPVCRAEPGNLDAMTTIRRGFHTLKGSGRMVGLTDLGDV
                     AWQCEQVLNKWLKDEKPATAPLRAFIELARESFSRWVSELQENSAAAIDGTEIAR"
BASE COUNT         1620 a         2910 c         3033 g         1301 t
ORIGIN      
        1 acgtggttgg tatcgccggc gaggcaggcg accacgctgg catcgcccga ctgcaccgcg
       61 cgcgccgcgc gccgcagcgc caccacgccg ctcgccccgc ccaggggaat gtggtcgagc
      121 cagcgcggcg cgagaccgag gtgctgcgtc aggccgatcg ccgtgtcggg ctgcagcgtg
      181 aagctggaaa tggtcagccc atcaatttca tttttggata attttgattg tttcaaaagc
      241 gaatccaggg cccgcgccag ccaccagtgc gccgagcgga tggaatagcg cacgtagggc
      301 accgtgaccg ggaccgcgac ggcgacgccc tcatagccgc ggcgcgtcat gtgcgcttct
      361 tgcggtcgcg caggtcgaag cagttcgacg ggttgaccgc gaagtcggcg agcttccctt
      421 tctgcacttt gttggtcgaa gtcaccggca gctcgtcgag gaaagcgacg tagcccggcg
      481 ccttgaagta cgaaagacgt tccaggcacc agtcctggat tgattttgcg gtttcaatat
      541 ttttctgttt ttgatcggta aggatcacgc acgccatgac ttcttcgtcc cgcacgggat
      601 cgggagcggc aatcacggcc acctgctgga ttgcggggtg ccccgcgagc gcggcctcga
      661 cttccagcgc ggcgatgttc tcgccggagc gccggatgat gttcttgcgc cggtcgacga
      721 agtgcagctg cccgtccggc ccgagccgca ccgcgtcccc ggtgtggaac cagcctccgt
      781 gccaggcctc ttcggtggcg gcctcgtcct tgtagtaccc cgaaaagaag cctttcctgg
      841 gatccggccc tttctgccgc accagcagct cgccagtttc ctcttccacg tggacttcga
      901 gagcatccgg agggaaaccg atgcatctcg atccgacgtg ccgcggctcg cggctcgccg
      961 cgatcagcgc gccgccgccg gtctcggtca tcgcccaggc ctcgatcagc gggaagccga
     1021 accgttcctc gaacgaggca tggtcctttg ggttggcgtt ggcgccgtag ccgaagcgca
     1081 cgcggtgcgc gcggtcgtac ggccccggcg cctggccgag caggatggcg ggcatcacgc
     1141 cgagatagtg cacgatggtg gcgtgggaga gggcgacctc ctgccaccag ctgcgcggat
     1201 ggaagcggtc gagctggatc acgcagccgc cggtcaggat catcgccacc gtcgacacgg
     1261 cgagcgaatt catgtggaac agcggcagcg gcgtgatgag ccgctcttcg ccctcgcgca
     1321 cctcgcacag gccgccttcg gcaagatagc gccgcgccac gttgagaaag taaaagttgg
     1381 tcaagaggca gcccttcggc ttgcccgtcg tgcccgaggt gtagagcagg gcgcattcgg
     1441 ctgcgtcgcc gggcgggggc gcggccggca gttctgcaaa attctcatcg actaccaggc
     1501 aggcttcgct gtgccgcagc acgtagtcga gctccgcctg gcggtactcg ggattgaggg
     1561 ggacgatgct tgcgccgagc gagttaagcg caaggaaatg caggaggaat tccggccggt
     1621 tccccagctt gagcgctacg cggtggcccg cgccatagcc gcgcgcgcgg tacctggcgg
     1681 ccagagcgcc gaccttctcg agagtctcgg cataggcgaa gcccggcggg tggaggaacg
     1741 gcctgccgcc gtggcggcgc gcggtgtgct cgaacgcggc gtagaccgat tccacccggg
     1801 cgattctagg cggtaaaaat cggtaagaaa taccgcccct aagtgtgagt ttctgataac
     1861 cctgatgtat ttcccgggga tagtcggata acgtctccct cgccgcgcaa catgctggaa
     1921 taatgtatta ttccacgtga atattttgcg gtaatagcct gatccgcaaa ctaataacaa
     1981 gatcgcggaa cggcccagcc cgaactcggt gcataagtag gaagcagaca gagaaagagg
     2041 gggagcgatc tccgtgagca cgacgaaccc cacgaaggtt ctcgtcatcg atgacagcaa
     2101 cacgatccgg cgcagcgcgg aaatgtttct gcgccaggcc ggctacgaag tcattctcgc
     2161 ggaagacgga ttcgacgcct tggccaagat aagcgaccac cagccgcaag tgattttcgt
     2221 cgacatcatg atgccgcgcc tcgacggcta tcagacctgc gcgctgatca agcagaaccc
     2281 ggtgctgaaa gccacgccgg tgatcatgct gtcgagcaag gacggcgtgt tcgaccgcgc
     2341 gcgcggcagg ctggcgggct ccgaccggta tctcaccaaa cccttcacca aggaagcgct
     2401 gatcgaggcg gtcgagcaga cggccaagcc cgccacgctg agctcttcct aattcctcga
     2461 agaaaggctc gttatgccca tcaagaacat tctcgtagtc gacgactctc ccaccgaccg
     2521 ccagcacctc tccgacatcc tgacgaagag cggctacaag gtcaccaccg ccaacagcgc
     2581 cgaagaggcg ctcgccaagg tgaagctgtc ggcgcccgac ctggtgctga tggacgtggt
     2641 gatgccggga cagaacggct accaggcgac gcgccagctc accaaggacg acgccaccaa
     2701 gcacatcccg gtcatcatct gcaccaccaa gggccaggag accgacaagc tctggggcat
     2761 gcgccagggc gcgcgcgact acatcgtcaa gccggtgatc cagtccgacc tgctcgccaa
     2821 gatcgcggcg ctcggctagg aacaggcccg gaaaagacat ggctgagcgg ctctccctca
     2881 aggactacca gcgcgggctg gcggagcgcc tgcgcgaggc aggcgccggg cgcatggcct
     2941 cgaagctcgg catgcagctc ggcaccgaga cctggctggt cgacctgggc gatgccggcg
     3001 aggtgatccc ggtgccggcg gtgacgccgg tgccgctgac gcggccctgg ttcaaggggg
     3061 tcaccaacgt gcgcggcaat ctctacagcg tgatcgactt cccggcgttc ctcggcgtgg
     3121 gcgccgtggc gctgggcgag cactcgcggc tgctgctgct cggcgagcgc ttccgcacct
     3181 cggcggcgct gctgatcgac cgctcgctcg ggctgcgcaa tcccgagcag ctcacgccgc
     3241 gcgcggccaa cggcgaggcg ccggcatggc tgcgcggcga gttcgacgat gcggaaggca
     3301 aggcatggaa ggagctagac gtgccgcaac tagtacggca cgcggacttc ttgtcggtaa
     3361 gcgcatgagg aggacagccg tgatgaagaa aatcgttttt gcaaccctgg cctgcgcggc
     3421 gctgcccgcg ccggcgcagc agccgggcgt cagcgcggac aggatcctgc tgggacaggc
     3481 cgcggtcttc accggcccgg ctgcgcagct gggaatccag atgcgcaacg gcatcaagtc
     3541 ctatttcgac ttcgtcaacg aaaagggcgg cgtgcacggc cgcaagctcg agctggtctc
     3601 ggaggacgac aagtacgagc cggcggtggc gaccaccgcc agccaggcgc tgatcgaaaa
     3661 gcacaaggtg ttcgcgctga tgggctacgt cggcaccccg accggggtgg cgcacctgaa
     3721 ggtgaccacc gctgccaagg tgccgctggt gggcatgttc accggcgccg aggtgctgcg
     3781 cgagccgatg agccgctacg tgttccacgt gcgcgccagc tactacgacg agaccgacaa
     3841 gatcgtcgag caggtggtgt cgaccggagg caagaagatc gcggtcttct accaggccga
     3901 cgcctacggc gaggccgggc gcatgggcac cgagatcgcg ctcaagaagc gcggcttgca
     3961 gatccactcg aacggcaccg tggagcgcaa caccgtcgcg gtcgagaagg cggtagagac
     4021 catccaggcc tctgagcccg acgccgtggt gatggtcggc gcctacacgg cctgcgccga
     4081 gttcatccgc cagatgaaga aggccggcag cggcgcgcag ttctacaacg tgtctttcgt
     4141 cggtagcgcc gcgctcgcca aggcgcttga taaggagggc gtcggcgtgg ccatttctca
     4201 ggtggtgccg ttcccctggg cgaccggcgt gccggtggtc aaggagtacc aggcgctggc
     4261 gaagaaggcc ggcttcgccg actacaactt cagcgcgatg gagggcttcc tggtcgccaa
     4321 ggtgatggtt gaaggcctgc gccgcaccgg caagaacctg acgcgcgaag gcttcgtcga
     4381 cacgatggag aagatgcagg acgtcgatat cggcggtttc ttcgtcacct attcccctaa
     4441 gagccacacg ggctcgaagt tcgtcgacct gacgatcatc ggccgtgaag gcaagttcct
     4501 gcgataagag aggcacacca tggcattcgc gctgaaactc ccattcttcg gacgccgcgc
     4561 cgacgcggaa gaggccgagg cgcccgcgcc cgcagccgag cggcccaagt cccgcggctt
     4621 cggcaccggc cgcaagctgc agatcctgat cacattgctg atcgtgctgc tcgcggtcga
     4681 cggcgtgatc gtcgcgctcg acgcgcggca gggcaccttc ggcacgctct acatcgcgag
     4741 cgttggcaag atccgcatgc tgtcgcagcg cctggcgaaa gcggcgcagc aggcctcgca
     4801 gggcaacatc gaggccttca agcagctcaa ggagtcgcgc gaggagttcg cctcgctgat
     4861 gaagctgctc accgacggcg ggcagacggc gggcaccagc ctgccgccga cttcggccgc
     4921 ggcgcggccg gcgctcgacg cgctcgacaa ggagtggaag aagaccgaga agaactcggg
     4981 cctggtggtc aacgagcagg cgaacctggt ggccctgggc aacgcggtgc gctcgatcaa
     5041 cgccagcaac cccacgctgc tcgagctggc cgacgagatc gcggcgatga gcgtgcaggc
     5101 cggcggctcg ctgcggcaga acgccatcac ctcgcagctg gtgatgctga cgcagcgcat
     5161 ggcgaagaac gcgaacacca tgctggcggg cgacgtcgtc gacccggagg tggcgttcct
     5221 gctgggcaag gacaccaaca ccttccgcga cacgctgcag ggcctgctct cgggcaacga
     5281 gggccagcgc atcacgcgcg tcggcgacgc cgagatgcgc ggcaagctct ccgagctcga
     5341 gtcgggcttc aaggagtacc agcgcgcggt gagcgacatc ctgggcaaca tgcagcgcct
     5401 ggtgaacgcc aagcgcgcga cgcgcgacat cttcaacgac agcgaggcgc tgctgcgcac
     5461 ctcggaagcg ctgaacgagc gctacgaggc cgagctgggc ggccggcaaa cgaacttcat
     5521 cgcgctggcg gtagtctcgc tgctcgcctt cgtcgtgctg ctcctggtgg gcaaggtgta
     5581 cgtcgacgac agccgcatcc gggcggagga cagcgagaag gtcaaccgca ccaaccagga
     5641 ggcgattctt cgcctgctcg acgagatcgg caacctggcg aacggcgacc tcacggtgcg
     5701 cgccagggtg accgaggaca tcaccggcgc catcgcggac tcgatcaact acacgatcga
     5761 cgagctgcgc cgcctggtgt cgggcatcac cgaggcctcc acccaggtga ccgcggcgac
     5821 gcaggaagcc tcgacggtga ccggccagct gctgcaggcg acgcagaagc agtcgaccga
     5881 gatccagggc gcgggccagt cggtcacgca gatggcgcag cagatgaccg acgtgtcggt
     5941 caaggcgaac gactcggcga aggtcgcgga gacctcggtg agcgccgcgc agaaaggcac
     6001 cgcggcggtg cagaacgcga tcaaggggat gaacgacatc cgcgagcaga tccaggagac
     6061 ctcgaagcgg atcaagcgcc tcggcgagag ctcgcaggag atcggcgaga tcgtgcagct
     6121 gatctcggac attaccgagc agaccaacgt gctggcgctg aacgccgcga tccaggcggc
     6181 ctccgccggc gaggcggggc gcggctttac ggtggtcgcg gaagaggtgc agcgcctggc
     6241 ggaacgctcg ggcgaggcca ccaagcacat cggcgcgatc gtcaagtcga ttcagcgcga
     6301 cacgcaggac gcggtcgagg ccatggagcg cagcacggcg aacgtggtgg ccggaaccaa
     6361 gaccgcggac gaagcggacc aggcgctgcg cgagatcggc tcggtgtcga acacgctcgc
     6421 cgacctgatt cgctcgatct cgacctcgac ccagcagcag tcggcttcgg cgacgcgcgt
     6481 ggcggcgaac atgaaggtga ttctgggcat cacgcagctc accaccgagg gcaccaagaa
     6541 aaccgccggc tccgccgcgc gcctgaccac cctcgccgac gggctgagga gctcggtctc
     6601 gggcttcaag ctggcctaaa gacctagaca gaaaagcgaa gagcgatact gatggcgaac
     6661 gccacgctcc tgtcctggat caacgccgag gtcgatcagg ccttgaagct ggtccgcgac
     6721 aacatcgcca aggtctcggg caagcccgag gacgacgcga tgctgcgcgt gtgccccgac
     6781 cacctgcacc aggtgagcgg ggcgctgcgc atggtggggc tggccggcgc gacgcgcttc
     6841 tgcgaggcga tcgagggcag cttctcgggc ctgtcgtcgc aggcgccgcg caacaacggc
     6901 gccgcgatcg gcaccatcga ccgcgcggtg ctggcgctga aggagttcgt ggcggacctg
     6961 gcgcgcggcc aggcggacgt gccgctgcgc ctgtatccgg tctacaagga gctggcgacc
     7021 ctctccggca agaacgacgc gtcggagaag gagctgttct tccccgacct cacgcagtcc
     7081 gcgccagcgc accccgaggc gaagaccctg ctgcaggcgg acctggtgcc gttcctgcag
     7141 gcgcagcgcg cggtcttcca gcgcggcatg ctcggctggc tgcgcaagca gccggccggc
     7201 ctcgaggaga tgcgccaggc gctcgacgcg ctgtaccgga tcgccgccca gctgcccgag
     7261 ccgcgcgcgc tgtggtgggc ggcgctcggc ctggtcgagg gcatgggcca gtcgcaggac
     7321 gccgactggc agacgagcgt caagccgctg tgcaacaaga tcgatttcca gatgcgcgat
     7381 ctggccgccg ggtcgagcaa ggtccaggac acgctgctgc gcgacctgct ctacgccgtc
     7441 gccaagtgca agccggtgac gccgcgcatc aaggagatca ggcagctgta ccagctcgag
     7501 agcctgttcc ccgaggcgcc caaggcgggc ggcggcgtga tggagttcga catcgagttc
     7561 ctcgaggtcg cgctgtacga cctgcattcg cgcctcgagg cgctgaaagg cgcctgggtg
     7621 cagtacgtct cgggcgagca gaagagcgcc gggcgcttcc gcgagctggt gggcgcgttc
     7681 aaggccaagg cgagcgagct cggcaaccag cacctgatca agctgctcga cgccatcggc
     7741 ctggtggccg ccaagctgcc cgacccgtac ccgcgccaga accagttcat ggtgatcgag
     7801 atggcctcgg cgttcctgct ggtggagcag gtcatcgacc atttcaccac gccgcccgac
     7861 gacctcgcgc cgcagatcgt gatcatgggg ggctggctgc tcgacgcggc gaagggcaag
     7921 tcgagcggcg aggtgccggc cggcatgcgg cccgacctca cccagcagat cggcgcgatg
     7981 cagctgcgcg cgcaggtcgc caaggagatc atcgccaacc tgcagcacgt cgagcaggtg
     8041 ctcgacgcgt acgtgcgcga cgcctccaag cgcgacacgc tcgccgcgct gccgccctac
     8101 ctgcgccaga tccacggcgc gctgatggtc ctgcgcttcg accgcgccgc cgaggcgctc
     8161 gccatctgcg agaagatgat cgccgcgtgc gccgagaagg agctggccga ttccgccgag
     8221 gacatcgact ggatcgccga gggcctgtcc agcctgggct tcttcctcga gccctgcctg
     8281 cacggccgcg agccggccga gcaggcgatc cagctgttct tcgagcgcta cggcaagcgc
     8341 tccggcgccg ccgaggtggc gccggcaacc cccgctgccg ccgccgccga gggcgcggac
     8401 ctgacgatca agctcgactt cgccgcgatg gcagccgacg tgacgcagaa gatgagcgct
     8461 gccgcggacg cgccgcgccc gtcggtggcg ccgccggtgc ggcgagcggc ggtcgatgcc
     8521 gagctgctcg gcatcttcct cgacgaggcc ggcgaggtgc tgcagacgat caacgacacg
     8581 gtgccggtat gccgcgccga gcccggcaac ctcgacgcca tgacgaccat ccgccgcggc
     8641 ttccacacgc tcaagggcag cggccgcatg gtggggctca cggacctggg cgacgtcgcg
     8701 tggcagtgcg aacaggtgct gaacaagtgg ctgaaggacg agaagcccgc caccgcgccg
     8761 ctgcgcgcgt tcatcgagct tgcgcgcgag tcgttctcgc gctgggtctc ggagctgcag
     8821 gagaattccg ccgccgccat cgacggcacc gagatcgcgc gcga
//
LOCUS       DASYJA010000425         1781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2423779, whole genome shotgun sequence.
ACCESSION   DASYJA010000425 DASYJA010000000
VERSION     DASYJA010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2423779"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..866)
                     /locus_tag="VGO02_09325"
     CDS             complement(99..866)
                     /locus_tag="VGO02_09325"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7477921.1"
                     /translation="MNALEASGVVKRYERDGKSLSIVEIAEFAVREGEFVTVIGPSGC
                     GKSTFLHIVGGFIPPESGSIRVYGKEVSGPGPDRGMMFQEFSLFPWRSVAGNIAWGLE
                     AQGFDKDRINEIVDRQLAMIGLTDFRNHYPAELSGGMKQRVALARVLAFDPKVLLMDE
                     PFGALDAQTREALQEDFILISKKLQTTVVFVTHSIDEALVLSDRIIVLSSRPGRIKKT
                     IVPPFAKSRAATDLRSLPEFAVCAQEVRNLLREERAP"
     gene            complement(863..1291)
                     /locus_tag="VGO02_09330"
     CDS             complement(863..1291)
                     /locus_tag="VGO02_09330"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7477922.1"
                     /translation="MARIRHIAITAEDPFATAELFKKGFGLQELSRGDSELAREVYLT
                     DGYINIAIVCWKRTKETPNPYPDGYGLDHFGFQVDDLEGAEKRAKQAGATPQPPPPVD
                     LSKLGDRIFFETKLQLAGVKFDLSGHGWPVTVEKKQAEKK"
     gene            complement(1305..>1781)
                     /locus_tag="VGO02_09335"
     CDS             complement(1305..>1781)
                     /locus_tag="VGO02_09335"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEV7477923.1"
                     /translation="PAPQLASADEFRALFAQFTDPRVVKLCSQLRSAGEIITTDVEEL
                     RQERWGEGRAVLLGDAVHAMTPNIGQGAGMAMEDAAVLAEELGSSSSIENSLRNYAQR
                     RKPRVETIVRVSRNVGTEGQMSGIIDCWLRERRVRRAGRDRAKMLAELEQLLVFEG"
BASE COUNT          274 a          583 c          597 g          327 t
ORIGIN      
        1 ccgaaagacg ttcggatggc tcttcagcca cgccttccaa tcggcgcgac gctccgcggg
       61 cggtcgcggc gtcaccggct tcggggcgac gacctcgctc atggcgccct ctcttcccgg
      121 agcaggttcc tgacttcctg cgcgcacacc gcgaactcgg gaaggctgcg caggtcggtg
      181 gccgcgcggc ttttcgcgaa cggcggaacg atcgtcttct tgatacgacc cggtcgcgaa
      241 ctgagaacga tgatgcggtc ggacagcacc agggcttcgt cgatgctgtg cgtgacgaag
      301 accaccgtcg tctgcagctt cttcgagatc aggatgaagt cttcctgcag cgcctcgcgc
      361 gtctgggcgt cgagggcgcc gaagggctcg tccatcagga ggaccttggg atcgaaggcg
      421 aggacgcggg cgagcgcgac gcgctgcttc atgccgccgg agagctcggc gggatagtgg
      481 ttgcggaagt cggtgaggcc gatcatcgcc agctgccggt cgactatctc gttgatcctg
      541 tctttgtcga agccctgcgc ttccaggccc caggcgatgt tgccggcgac gctgcgccag
      601 ggaaacaggg agaactcctg gaacatcatg ccgcggtcgg ggccggggcc gctcacttcc
      661 ttgccgtaga cgcggatgct gccgctctcc ggcgggatga agccgccgac gatgtgcagg
      721 aaggtgctct tgccgcagcc gctcgggccg atgacggtga cgaactcgcc ctcgcgcacg
      781 gcgaactcgg cgatctcgac tatggagagg gacttgccgt cgcgctcgta gcgcttgacg
      841 acgccggagg cctccagcgc gttcacttct tttcggcctg cttcttttcc actgttaccg
      901 gccagccgtg gccggagagg tcgaacttga cgccggcgag ctgcagtttg gtctcgaaga
      961 agatgcggtc gccgagcttg gacaggtcca ccggcggcgg cggctgcggc gtggcgccgg
     1021 cctgcttcgc gcgcttctcg gcgccctcga gatcgtcgac ctggaagccg aagtgatcca
     1081 ggccgtagcc gtcgggatac gggttgggag tctccttggt gcgcttccag cagacgatgg
     1141 cgatgttgat gtagccgtcg gtgaggtaga cctcgcgcgc gagctcgctg tcgccgcgcg
     1201 agagctcctg cagcccgaag cccttcttga acagctccgc ggtcgcgaac ggatcttccg
     1261 ccgtgatcgc aatgtgcctg atcctcgcca tcgtgcttct cctttcagcc ttcgaacacc
     1321 agcagctgct cgagctccgc cagcattttt gcccggtccc ggccggcgcg ccgcacgcgg
     1381 cgctcgcgca gccagcagtc gatgatgccc gacatttggc cttcggtgcc cacattgcgc
     1441 gacacgcgca cgatggtctc gacgcgcggc ttgcgccgct gggcgtaatt tctcaaagaa
     1501 ttttcaatac tggaagaaga accaagttct tctgcaagga ccgcggcgtc ctccatggcc
     1561 atgccagcgc cctggccgat attgggcgtc atggcatgca ccgcgtcgcc cagcaggacc
     1621 gcgcggccct cgccccagcg ctcctggcgc agctcctcga catcggtggt gatgatctcg
     1681 ccggcggagc gcagctggga acagagcttc accacgcgcg gatcggtgaa ctgcgcgaac
     1741 agggcgcgga actcgtcggc gctggcgagc tgcggcgcgg g
//
LOCUS       DASYJA010000426         1969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_883131, whole genome shotgun sequence.
ACCESSION   DASYJA010000426 DASYJA010000000
VERSION     DASYJA010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883131"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VGO02_09340"
     CDS             complement(<1..774)
                     /locus_tag="VGO02_09340"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEV7477924.1"
                     /translation="MDDLFISYSRRDNEQGRVTQLVERIKAGFATIAGRELAVFFDEH
                     DILGMEDWRQRILHGLRESRLLLTCISPGYLASEWCQAEFVEYLKYEIGHRHGSHGVA
                     PVYFIEIEGWKFQGFEQQCAAWVAELRQRNYFDLRLWHDLGEEALRDPAVQERLDQLT
                     RQIAETIQRGERAERSLGNVDAPNPHFIGRHANLRQLRQNFVTPGQLGVVTAVNGLGG
                     LGKSALAVEYAYSFAHEYGGGRWQVPCAGQEDLGLALAGL"
     gene            1077..>1969
                     /locus_tag="VGO02_09345"
     CDS             1077..>1969
                     /locus_tag="VGO02_09345"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEV7477925.1"
                     /translation="MEDSIFTRRQFLERTGMGFGAMSLASVLGLGAGASAAPSAAVLS
                     NPLAPKEPPFPAKAKAVIHIFAEGGPSHVDTWDPKPELAKYADKTLPGLEGLAFPSPF
                     KFNKMGKSGIEVSEVFPKLGQQVDEMAIIRSLWTDIPAHEVAQRFMNTGSLQLPKPSM
                     GSWVVYGLGTENQNMPGFITLSGKPEWRQSSFLPGVYQGCNVNYSSTMRLDEVLQNIK
                     NQFSGVDRQRRQLDLVHKLNDLHAEALQKDAQLEARIEAFEMAFKMQAEATDAFDISK
                     ESAETKDLYGASDMGSKLLVAR"
BASE COUNT          360 a          638 c          639 g          332 t
ORIGIN      
        1 gaggccggcg agggcgaggc cgaggtcctc ctggccggcg cacggcacct gccagcggcc
       61 gccgccgtat tcatgggcaa aggagtaggc gtactcgacg gcgagcgcgc ttttgcccag
      121 cccgccgagc ccgttgacgg cggtgacgac gccgagctgg ccgggcgtga cgaagttctg
      181 gcgcagttgc cgcagatttg cgtggcggcc gatgaagtgc gggttgggag catcgacatt
      241 gccgagactg cgctccgccc gctcgccgcg ctggatggtt tcggcgatct ggcgggtgag
      301 ctggtccaga cgctcctgca cggcggggtc gcgcagcgcc tcctcgccca ggtcgtgcca
      361 caggcggagg tcgaagtagt tgcgctgccg cagctcggcc acccacgcgg cgcactgctg
      421 ctcgaagccc tgaaacttcc acccctcgat ctcgatgaag tagaccggcg cgacgccatg
      481 ggagccatgc cgatggccga tctcgtactt gaggtactcg acgaactcgg cctggcacca
      541 ttcgctcgcg aggtagcccg gggagatgca ggtgagaagc aggcgggatt cgcgcaagcc
      601 gtgcaggatg cgctgccgcc aatcctccat gccgaggatg tcgtgctcat cgaagaacac
      661 ggccagctcg cgcccggcga tcgtcgcgaa gccggccttg atgcgctcga cgagctgcgt
      721 gacgcgcccc tgctcgttat cgcggcggga gtaggagatg aagaggtcgt ccatggtgtc
      781 tccggaagcg gcacggtagc gaggccgggg caggggcgta acgcagaaag ttcgaccgaa
      841 gccggcggcg ttgcagccac tgccctgtgc gccgtctcgt aaccgcacca tggcgagcgg
      901 aatgggcggt cctccctccg gcccgattct gaacgttctg atcaaacaaa agcgacaatt
      961 tcggcgaaac tttcccggat cgtggtagtt aatagctcgt tcgcattcca ttccacactt
     1021 ccatgaacga ttcctgcgct tccacttcca agtacactcg cgaacatacc tctcccgtgg
     1081 aggactccat tttcacccgc cggcaattcc tggaacggac gggcatgggc ttcggggcga
     1141 tgagtctcgc ctcggtcctc ggcttgggcg ccggcgcctc ggccgcgccc tcggcggccg
     1201 tgctgtccaa tccgctggcg ccgaaggagc cgcctttccc ggccaaggcc aaggcggtga
     1261 tccacatctt tgccgaaggc ggcccgtcgc acgtcgatac gtgggatccg aagccggaat
     1321 tggcgaagta tgcggacaag acgctgccgg gactggaagg gctcgcgttc ccgtcgccgt
     1381 tcaagttcaa caagatgggc aaatcgggga tcgaggtcag cgaggtgttc ccgaaactcg
     1441 gccagcaggt cgacgagatg gcgatcattc gctccctgtg gaccgacatc ccggcgcatg
     1501 aagtggcgca gcgcttcatg aacaccggct cgctccagct cccgaagccc tcaatgggct
     1561 cctgggtggt gtacggcctc gggaccgaaa accagaacat gcccgggttc atcacgctca
     1621 gcggcaagcc ggaatggcgc cagtcctcct ttcttccggg cgtttaccag ggctgcaacg
     1681 tgaactacag ctcgaccatg cggctggatg aggtgctgca gaacatcaag aaccagttct
     1741 ccggcgttga ccgccagcgc cgccagctcg acctcgtcca caagctcaac gatctgcatg
     1801 ccgaggcgct gcaaaaggac gcgcagctcg aagcgcgcat cgaggccttc gagatggcgt
     1861 tcaagatgca ggccgaagcc accgacgcct tcgacatctc caaggaatcc gcggaaacca
     1921 aggatctgta cggcgcgagc gacatgggct ccaagctcct cgtcgcccg
//
LOCUS       DASYJA010000427         2477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1606543, whole genome shotgun sequence.
ACCESSION   DASYJA010000427 DASYJA010000000
VERSION     DASYJA010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606543"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..704
                     /locus_tag="VGO02_09350"
     CDS             33..704
                     /locus_tag="VGO02_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV7477926.1"
                     /translation="MDQILEVFFNVAVFERAWPFLWRGVQMTFALSLVIVPMGVMSGL
                     AVAMLYHLHNKWLNRLLMAYVDFFRAFPPLVLLLFIYGGLPFLGLDLPLFATVALAFL
                     LNTSSYYGEIFRAGLESVPAGQVEAARSTGLSRWQTMVWVVLPQATRNVLPDLVSNTL
                     EVIKLTTLASVVGFTELLNNARNAQALTYNATPIVAAALIYLIVLWPLVRLVSRLEHK
                     SIALR"
     gene            720..1382
                     /locus_tag="VGO02_09355"
     CDS             720..1382
                     /locus_tag="VGO02_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEV7477927.1"
                     /translation="MSETILVINPNSTERMTREIDAACAPLRIPGGPGIETATLKEGP
                     PGIESQQDVDGLIPPLVAFVSQKKNYDAFVIACYSDPGLHSVREVTRKPVLGIAECGI
                     LTALTLGHKFGVIAILQKSIPRHMRYMGALGVMDRLAGELPVGLGIAEMADKKKTFAR
                     MVEVGKELKDEHGANVIVMGCAGMAEHRKPLQDAVGLPVVEPTQAAVTMAIGRVRLGW
                     EA"
     gene            complement(1385..1993)
                     /locus_tag="VGO02_09360"
     CDS             complement(1385..1993)
                     /locus_tag="VGO02_09360"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEV7477928.1"
                     /translation="MHLEFAQLPPLERYKLLIGLVIPRPIAWISTRSANGVANCAPFS
                     FFNVFSEDPPLCVIGINPRSDGAMKHTLKNIRRTREFVVNLADESTANAMHVSSYEFS
                     EEESEFEKTGLTPAPALRVQHPRIAEAAASLECRLYKLLEISATRELVLGEILVVHAR
                     DGIIDPKTKRISEENYRPVGRLFANRYCTTRQRFNLPGDLPK"
     gene            complement(1993..2280)
                     /locus_tag="VGO02_09365"
     CDS             complement(1993..2280)
                     /locus_tag="VGO02_09365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEV7477929.1"
                     /translation="MFVLQVSIRIKPENVDAFMKKCIENGNAAREEPGCRQFDILVDP
                     GDQTRVMLYEVYNDEKAFGAHQATPHFKKYVAEAVPLLASRERMVWQRASP"
     gene            complement(2291..>2477)
                     /locus_tag="VGO02_09370"
     CDS             complement(2291..>2477)
                     /locus_tag="VGO02_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019651946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-carbamoyl-D-amino-acid hydrolase"
                     /protein_id="HEV7477930.1"
                     /translation="PTGEIAVKSQTEEDEVICTNADIDLAANLKKTMFNFAAHRRPEH
                     YKLIVERVGAEVTPANK"
BASE COUNT          381 a          787 c          828 g          481 t
ORIGIN      
        1 ggtcgagacg cgcttcgcct ggaagcgcta gcgtggacca gatcctcgag gtgttcttca
       61 acgtcgcggt cttcgagcgc gcctggccgt tcctctggcg cggcgtgcag atgaccttcg
      121 cgctctcgct ggtcatcgtg ccgatgggcg tgatgagcgg cctggccgtg gcgatgctgt
      181 accacctgca caacaagtgg ctcaaccgcc tgctgatggc gtacgtagat ttcttccgcg
      241 cctttccgcc gctggtgctg ctgctcttca tctacggcgg gctgccgttc ctcggcctgg
      301 acctgcccct gttcgccacg gtggcgctcg ccttcctgct gaatacctcg agctattacg
      361 gcgagatctt ccgcgccggg ctggagagcg tgccggccgg gcaggtcgag gcggcgcgct
      421 ccaccgggct gtcgcgctgg cagaccatgg tctgggtggt cctgccgcag gcgacgcgca
      481 acgtgctgcc cgacctggtg agcaacacgc tggaagtgat caagctcacc acgctcgcca
      541 gcgtcgtcgg cttcaccgag ctgctgaaca acgcgaggaa cgcgcaggcg ctgacctaca
      601 acgccacgcc gatcgtcgcg gcggcgctga tctacctgat cgtgctctgg ccgctggtgc
      661 gcctggtttc gcgcctggag cacaagagca tcgccctgcg ctagaggtat catcgcgcca
      721 tgtccgaaac gatcctcgtc atcaatccca actcgaccga gcggatgacg cgcgagatcg
      781 acgcggcctg cgcgccgctg cgtatccccg gtgggcccgg catcgaaacc gctacgctga
      841 aagaaggtcc tccgggcatc gaatcgcaac aggatgtgga tggcctgatc ccgcccctgg
      901 ttgcttttgt ttcacagaag aagaactacg acgcctttgt gattgcctgt tatagcgatc
      961 caggattgca ctcggtgcgc gaggtgacac gcaagcctgt ccttggcatc gccgagtgcg
     1021 gcatcctcac tgcgctcacg ctcggccaca agttcggcgt catcgccatc ctgcagaaat
     1081 ccatcccccg gcatatgcgc tacatgggcg cgctgggcgt gatggaccgg ctggccggcg
     1141 agctgccggt cggcctgggc atcgccgaga tggccgacaa gaagaagacc ttcgcccgca
     1201 tggtcgaagt ggggaaagag ctgaaagacg agcacggcgc caacgtcatc gtcatgggct
     1261 gcgccgggat ggcggagcac cgcaagccgc tgcaggacgc ggtcggcctg ccggtggtcg
     1321 agccgaccca ggcggcggtg acgatggcga tcggccgcgt gcgcctgggc tgggaagcct
     1381 gacgttactt gggtagatcg ccgggcagat tgaaccgctg ccgggtcgtg cagtagcggt
     1441 tcgcgaagag ccgtccgacg ggcctgtagt tttcctcgga aattcgcttg gtcttggggt
     1501 cgatgatgcc atcgcgcgcg tgcactacca gtatttcgcc caataccagc tcgcgcgtgg
     1561 cgctgatctc gagcagcttg tacagcctgc attcgaggga cgcggcggcc tcggcgatgc
     1621 gcgggtgctg cacgcgtagc gccggcgcgg gcgtgaggcc ggtcttctcg aactcacttt
     1681 cctcctcgct gaactcgtag ctcgagacgt gcatggcgtt ggccgtcgac tcgtcggcca
     1741 ggttgacgac gaactccctg gtgcggcgga tgttcttcag cgtgtgcttc atggcgccgt
     1801 cgctgcgcgg gttgatgccg atcacgcaga gcggcgggtc ctcgctgaac acgttgaaga
     1861 aggagaacgg cgcgcagttg gcgacgccgt tcgcgctgcg ggtggagatc caggcgatcg
     1921 gccgcgggat caccaggccg atcagcagct tgtagcgctc gaggggcggg agctgcgcga
     1981 actccaggtg cattacggcg aggcgcgctg ccagaccatg cgctcgcgcg aggcgagcag
     2041 cggcaccgcc tcggcgacgt acttcttgaa gtgcggcgtt gcctggtgcg ccccgaatgc
     2101 cttctcgtcg ttatagacct cgtagagcat cacccgggtc tggtcccccg ggtcgaccag
     2161 gatgtcgaac tgccggcagc cgggctcctc gcgcgccgcg ttgccgttct cgatgcactt
     2221 cttcatgaac gcgtcgacgt tctcgggctt gatcctgatg ctcacctgca ggacgaacat
     2281 gccgggctcc ttacttgttg gcgggcgtca cttcggcgcc gacccgctcg acgatcagct
     2341 tgtagtgctc ggggcggcgg tgggcggcga agttgaacat cgttttcttg aggttggcgg
     2401 cgaggtcgat atcggcgttg gtgcagatga cctcatcctc ctcggtctgg ctcttcaccg
     2461 ctatttcgcc agtcggc
//
LOCUS       DASYJA010000428         4913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_336125, whole genome shotgun sequence.
ACCESSION   DASYJA010000428 DASYJA010000000
VERSION     DASYJA010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336125"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VGO02_09375"
     CDS             complement(<1..883)
                     /locus_tag="VGO02_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006758876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7477931.1"
                     /translation="MAPVISISKLTKTYASGLQALKEVSLDIRRGEIFALLGPNGAGK
                     TTLINIVCGIVTPTSGTVLADGHDIVREYRAARSKIGLVPQELHTDMFETTWGTVRFS
                     RGLFGRAPNAAHLEKVLRELSLWDKRNDKIMTLSGGMKRRVLIAKALAHEPQILFLDE
                     PTAGVDVELRRDMWALVRRLREGGVTIILTTHYIDEAEEMADRIGVISKGELIVVEEK
                     AVLMKKLGKKQLSLQLQHPLAAVPSDLSSFSLILKEGGNELEYTFDAHEKQGIAQLLR
                     RLSELGIGFKDLHTSQSS"
     gene            complement(895..3390)
                     /locus_tag="VGO02_09380"
     CDS             complement(895..3390)
                     /locus_tag="VGO02_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV7477932.1"
                     /translation="MQKERARSLLSGIPLGRVLIALGVVLVAINIAAAVWDARTAYER
                     VERRAQRDFSNLTRLLAEQTAASLESVDFMLRDAARAGSAQAVAARLPRLGEDLAHVS
                     QVAAFVVLDADGTVLARTGESAVPDAEIGALPFFAEHRNSRAPGLRLSGPYQGGAERA
                     QWRFLLSRPIARPGGAFGGAVAAAIEVESFDRLYRAIDVGDGGFIALITHDGILMTRV
                     PDPGSVRGQPYPPGVIMPAVREAGRFEGWTRSPITGEQVLLAASAVRGFPLVVASGSR
                     EAAVFAPWRDEAWLIFDRTLLTSTAMFALIALAAWGLARRERALARSWRKYQSMIEHS
                     SDAVILTRPKAGGVLYASPAIGRVLGYSIEDLRGREVIDLIHPGYREAALKAREELLG
                     VPGKVAVDETKVLHKDGSYRWIELTRSNLLHEPSVGSVVMNFRDITERKAAEAERLRL
                     EQRLRQAEKLEAVGRLAGGIAHDFNNILGGILGYAEMLAESAPAGSPLKRYAGNVLTG
                     ANRASDLVAQILSYSRSQRGQRAPVDLGRVVAETIELVRGSLPPGIRLEAALPAAPLH
                     VVGDETQLHQVAMNLCTNAIHAMSRSGTIRVSLEAVDEATDRVFAHATLRSGSYVCLA
                     VQDDGSGMDKATLARIFEPFFTTKEVGKGTGLGLALVYGIVTDSGGAIDVASAPGRGS
                     RFAIYLPRVEAPAAAEDLAAEPLARGNGERVLVVDDEEALVAVTSEVLKRIGYEPLGC
                     ADGAAALAAFEAADGSIDAAILDEVMPEISGTDLAQRLRRQRPNLPIVLVSGYTGPML
                     VERALGVGITEILKKPVQSRDMAAALARALKRA"
     gene            complement(3399..3812)
                     /locus_tag="VGO02_09385"
     CDS             complement(3399..3812)
                     /locus_tag="VGO02_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7477933.1"
                     /translation="MNDESKPTGEILVIDDDAIMRDLIADWLEAAGYKVRKVAGCEAG
                     VAELERSPPALVVSDMWMPGPCGAEAIQWMQREFPAVRIIAVSGHFGFGHGCSRDAAV
                     AAGAAKALSKPVKRADLLAAVAELIGSPVTSVTSK"
     gene            3971..4684
                     /locus_tag="VGO02_09390"
     CDS             3971..4684
                     /locus_tag="VGO02_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7477934.1"
                     /translation="MPHVLAVDDDPTIRQLIADYLGQNELRVTTVADGAAMRAVLAEE
                     VVDLIVLDLKLRAEDGMALARSMRDESAIPIIMLTGRSEEADRVMGLELGADDYLTKP
                     FSPRELLARIRTVLRRRRTEVRQGRPDGIRAYRFDGWELNLNTRRLVASDGREAPLSN
                     GEFALLVVMLGAPQRILTRDQLLDLSRLHNDEVFNRSVDVQIMRLRRKIEKDPAQPRY
                     IRTERGTGYLFGVPVETVY"
     gene            complement(4681..>4913)
                     /locus_tag="VGO02_09395"
     CDS             complement(4681..>4913)
                     /locus_tag="VGO02_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477935.1"
                     /translation="PEARVAAIQKNRILLKDGVAIAALEGGELRRLAPSELTDDALKA
                     LAARPSHRPLHPYLRSLSGRKPKPPSIVRMTF"
BASE COUNT          749 a         1726 c         1674 g          764 t
ORIGIN      
        1 gcgagctctg gctggtatgc aggtccttga agccgatgcc gagctcgctc agccggcgca
       61 gcagctgggc tatgccttgc ttctcgtgcg catcgaaggt gtactcaagc tcgtttccac
      121 cttctttcaa aatcaaggaa aaacttgaaa gatcggacgg aactgcagcg agcggatgct
      181 gcagctgcag gctgagctgc ttcttgccga gctttttcat cagcacggcc ttctcctcca
      241 ccacgatcag ctcgcccttg ctgatcacgc cgatgcggtc ggccatctcc tcggcctcgt
      301 cgatgtagtg ggtggtgagg atgatggtga ccccgccctc gcgcagccgc cgcaccagcg
      361 cccacatgtc gcggcgcagc tcgacgtcga cgcccgcggt gggctcgtcg aggaacagga
      421 tctgcggctc gtgggcgagc gccttggcga tcagcacgcg ccgcttcatg ccgccggaga
      481 gggtcatgat cttgtcgttg cgcttgtccc acagcgacag ctcgcgcagc accttttcga
      541 ggtgcgccgc attcggcgcg cggccgaaga gcccgcggct gaagcgcacc gtgccccagg
      601 tggtctcgaa catgtcggtg tgcagctcct gcggcaccag gccgatcttc gagcgggcgg
      661 cgcggtactc gcgcacgatg tcgtggccgt cggcgagcac cgttccgctg gtgggcgtca
      721 cgatgccgca gacgatattg atcagggtgg tcttgcctgc cccgttgggg ccgagcagcg
      781 cgaagatctc tccgcggcga atgtcgaggc tgacttcctt cagcgcctgc aacccggagg
      841 cgtaggtctt cgtcagcttc gagatcgaaa tgaccggcgc cacggtgcgg cgcctcaggc
      901 gcgcttcagg gcgcgcgcca gcgccgcagc catgtcgcgc gactgcaccg gcttcttgag
      961 gatctcggtg atgccgacac caagcgcgcg ttcgaccagc atcgggccgg tatacccgct
     1021 caccaggacg atcggcagat tgggccgctg tctgcgcagg cgctgcgcga gatcggtgcc
     1081 ggagatctcc ggcatcacct catcgaggat ggccgcgtcg atgctgccgt cggccgcctc
     1141 gaacgcggcg agcgcggcgg cgccatcggc gcagcccagc ggctcgtagc cgatgcgctt
     1201 caggacctcg gaggtcacgg cgacgagcgc ttcctcgtcg tcgacgacca gcacccgctc
     1261 gccgttgccg cgcgcgagcg gctccgcggc caggtcttcc gcggcggcgg gtgcctcgac
     1321 gcgcggcaga tagatggcga agcggctgcc gcgcccgggg gcgctggcca cgtcgatggc
     1381 gccgcctgaa tccgtgacga tgccgtacac cagcgcgagg cccagcccgg tgcccttgcc
     1441 cacttccttg gtggtgaaga acggctcgaa gattcgcgcg agggtcgctt tgtccatgcc
     1501 gctgccgtcg tcctgcaccg ccaggcagac ataagagccg gaacgcagcg tggcgtgcgc
     1561 gaatacacgg tcggtcgctt cgtcgacggc ctcgagcgag acgcgtatcg tgccggagcg
     1621 gctcatggcg tggattgcgt tggtgcacag gttcatcgcc acctggtgga gttgcgtctc
     1681 atcgcccacg acatgcagag gcgccgcggg cagcgcggcc tccaggcgga tccccggcgg
     1741 cagtgagccg cgcaccagct cgatcgtctc ggcaaccacg cggccgaggt cgaccggggc
     1801 gcgctggccg cgctggctgc ggctatacga gaggatctgc gccaccaggt cgctcgcgcg
     1861 gttcgcgccg gtcagcacgt tgcccgcgta gcgcttgagc ggcgagccgg ccggcgcgct
     1921 ctcggcgagc atctcggcgt agccgaggat gccgcccagg atgttgttga aatcgtgcgc
     1981 gatgccgccg gcgaggcgcc cgaccgcctc cagcttctcc gcctgccgca gccgctgctc
     2041 caggcgcagg cgctcggcct cggctgcctt gcgctcggtg atgtcgcgga agttcatcac
     2101 caccgagccg acgctcggct cgtgcagcag gttgctgcgc gtgagctcga tccagcggta
     2161 cgagccgtcc ttgtgcagca ccttggtctc gtcgaccgcg accttgcccg ggacgcccag
     2221 cagctcctcg cgcgccttga gcgcggcttc gcggtacccg gggtggatga gatcgatcac
     2281 ttcgcgcccg cgcaggtcct cgatgctgta gccaagcacg cgcccgatcg ccgggctggc
     2341 atagagcacc ccacccgcct tcgggcgggt gaggatcacc gcgtccgagc tgtgctcgat
     2401 catcgactgg tatttgcgcc agcttcgcgc cagcgcccgc tcgcggcgcg caaggcccca
     2461 ggcggcaagc gcgattagcg cgaacatggc agtggaagtg agcagcgtgc ggtcgaagat
     2521 cagccaggcc tcatcgcgcc agggcgcgaa aaccgccgcc tcacgactgc cgctggcgac
     2581 gaccagcgga aagccgcgca ccgcagaggc ggcaaggagg acctgctccc cggtgatcgg
     2641 gctgcgcgtc cacccctcga agcgtccggc ctcgcgtacg gcgggcatga tgacgcccgg
     2701 gggatacggc tggccgcgca cgctgcccgg atccggcacc cgcgtcatga ggatgccgtc
     2761 gtgcgtgatg agggcgatga acccgccgtc ccccacatcg atcgcgcgat agagccggtc
     2821 gaagctctcc acctcgatcg ccgccgcgac ggcgccgccg aacgcgccgc ccgggcgggc
     2881 gatcggcctc gacagcagga agcgccactg cgcgcgctcg gcgccgccct ggtaaggccc
     2941 gctcaggcgc aatcccggcg cgcggctgtt gcggtgctcg gcgaagaagg gaagcgcccc
     3001 gatctccgcg tcgggcactg ccgactcgcc cgtgcgggcg agtaccgtgc cgtctgcgtc
     3061 cagcaccacg aaggccgcga cctgcgaaac gtgcgccagg tcctctccga ggcgcggcag
     3121 ccgggccgcc acggcttgcg cgctgccagc ccgcgcggcg tcgcgcagca tgaagtcgac
     3181 ggattcgagc gaggccgcgg tctgctcggc gagcagccgc gtcaggttgg agaaatcgcg
     3241 ctgcgcgcgg cgctcgacgc gctcgtacgc ggtgcgtgcg tcccagacgg cggcggcgat
     3301 gttgatcgcc accagcacca cgccgagcgc gatcaggacg cgacccagcg ggatgcctga
     3361 aaggagggag cgcgcgcgct ccttctgcat ggctgccttt acttggaggt gacggaggtg
     3421 accggcgagc cgatcagctc ggcgaccgcg gcgagcaggt cggcgcgttt caccggcttg
     3481 ctgagcgcct ttgccgcgcc cgccgccacg gcggcgtccc ggctgcagcc gtgcccgaag
     3541 ccgaaatggc cggagaccgc gatgatgcgc accgccggga actcgcgctg catccactgg
     3601 atcgcttcgg cgccgcaggg gccgggcatc cacatgtcgc tcaccaccag cgccgggggc
     3661 gagcgctcga gctcggcgac cccggcctcg cagccggcca ccttgcgtac cttgtagccc
     3721 gccgcctcga gccagtccgc gatgaggtcg cgcatgatcg cgtcatcgtc gatgaccagg
     3781 atttcgcccg tcggcttgct ttcgtcgttc acgcacccat tccactacac aggaattgcg
     3841 tgcaatttac agttaccatg gtgttacgtt acacctgtaa cggcgtttgg cacgcagcgg
     3901 ctagactctg cgcgccgtga gaagccaagt gagcacgact tcacgacccg aggccccttc
     3961 ccaggtggcc atgccgcatg tgttggccgt cgacgacgac ccgacgatac gccagctcat
     4021 cgccgactac ctcggccaga acgagctgcg cgtaaccacc gtggccgacg gcgccgcgat
     4081 gcgcgcggtc ctggccgagg aagtggtcga cctcatcgtg ctggacctca agctgcgggc
     4141 cgaagacggc atggcgcttg cccgcagcat gcgcgacgaa tcggcgatcc cgatcatcat
     4201 gctgaccggc cgcagcgaag aggccgaccg cgtcatgggc ctggagctgg gcgccgacga
     4261 ctacctgacc aagccgttca gcccgcgcga gctgctcgcg cgcatccgca ccgtgctgcg
     4321 ccgccggcgg accgaggtgc gccagggccg ccccgacggc atccgcgcct accgcttcga
     4381 cggctgggag ctcaacctca acacgcgccg cctggtcgcc tcggacggtc gcgaggcgcc
     4441 cctcagcaac ggcgagttcg ccctgctggt ggtcatgctg ggcgcgccgc agcgcatcct
     4501 gacgcgagac cagctgctcg acctatcgcg cctgcacaac gacgaagtct tcaaccgctc
     4561 ggtcgacgtg cagatcatgc ggctgcggcg caagatcgag aaggacccgg cgcagccgcg
     4621 ctacatcagg accgagcgcg gcaccggcta cctgttcggc gtgccggtcg agacggtcta
     4681 ctagaaggtc atccgcacga tcgacggcgg cttcggcttg cggccggaga gcgagcgcag
     4741 gtagggatgc agcggccggt ggctcgggcg cgccgcgagc gccttgagcg catcgtcggt
     4801 gagctccgaa ggcgccaggc gccgcagctc gccgccttca agcgcggcga ttgctactcc
     4861 atccttcaaa agaattctat ttttttgaat cgcagccacg cgagcctccg gtg
//
LOCUS       DASYJA010000429         1741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1063957, whole genome shotgun sequence.
ACCESSION   DASYJA010000429 DASYJA010000000
VERSION     DASYJA010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063957"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /locus_tag="VGO02_09400"
     CDS             <1..1449
                     /locus_tag="VGO02_09400"
                     /inference="COORDINATES: protein motif:HMM:NF044969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEV7477936.1"
                     /translation="DALRIDGGELRCKVVAEGGNLGFTQRGRIEAAFAGVRLNSDAID
                     NSAGVDTSDHEVNIKILLGLAISDGEMTEKQRNALLAQMTDDVAMLVLRDNYFQTQAL
                     AVGARLGPSYLDQEARFIRFLEKTGNLNRAIEFLPAEDEIAERKVRGAGLTTPERAVI
                     FAYSKMWLFDEIMASDLPEDPWIGTALARYFPALLREKFGAYIPRHPLKREIVATHVL
                     NSMVNRVGSTFVHRLSERTGARPAQVVRAYLAAREVLGDVAVWQQVEALDNKVPDAVQ
                     SEMLIEEGWLTARATTWFLRSRRLAEPLEHTFRRFIPAVQALRARLEPEAAASPQATT
                     WIAAGVPSPLALRVASADHLIAALDVAEVTETVQRNFDEVSEVHVGIGTRLGLSRLRL
                     QINELPSDSHWQALAKAALGDDLAGLQRALAQDVLQQGLGSPAELLATWETGNAIALE
                     RAQRLLAELADAKGGADLSMLSVALRELRNLG"
BASE COUNT          274 a          611 c          599 g          257 t
ORIGIN      
        1 gatgcactgc gcatcgacgg tggcgagctg cgctgcaagg tcgtggccga gggcggcaac
       61 ctcggcttca cccagcgcgg gcgcatcgag gcggcattcg ccggcgtgcg cctcaactcc
      121 gacgcgatcg acaactcggc cggggtcgat acctcagacc acgaagtcaa catcaagatc
      181 ctgctcggcc tcgcgatcag cgacggcgag atgaccgaga agcagcgcaa cgccctgctc
      241 gcgcagatga ccgacgacgt tgccatgctg gtgctgcgcg acaactactt ccagacgcag
      301 gcgcttgcgg tcggcgcgcg cctcggtccg agctacctcg accaggaggc gcgcttcatc
      361 cgcttcctcg agaagaccgg gaacctgaac cgcgccatcg agttcctgcc ggccgaagac
      421 gagatcgccg agcgcaaggt gcgcggcgcc ggcctcacca cccccgagcg ggcggtgatc
      481 ttcgcttaca gcaagatgtg gctgttcgac gagatcatgg cctccgacct gccggaggac
      541 ccctggatcg gcaccgcgct ggcgcgctac ttcccggcgc tgctgcgcga gaagttcggt
      601 gcctacatcc cgcgccatcc gctcaagcgc gagatcgtgg ccacgcacgt gctcaacagc
      661 atggtcaacc gcgtcggttc gaccttcgtg catcgcctgt cggagcgcac cggcgcgcgg
      721 ccggcgcagg tggtgcgcgc ctacctggca gcgcgcgagg tgctaggcga cgtcgccgtc
      781 tggcagcagg tcgaggcgct cgacaacaag gtgcccgacg ccgtgcagtc ggagatgctg
      841 atcgaggagg gctggctcac cgcgcgtgcc accacgtggt tcctgcgctc gcggcgcctg
      901 gccgagccgc tggagcatac gttccgccgc ttcatcccgg cggtgcaggc gctgcgcgcg
      961 cggctcgagc cggaggccgc cgcgtcgccg caggcgacca cctggatcgc cgccggcgtg
     1021 ccgtcgccgc tggccctgcg cgtggccagc gccgaccacc tcatcgccgc actcgatgtc
     1081 gccgaggtga ccgagacggt gcagcgcaac ttcgacgagg tgagcgaagt tcatgtcggc
     1141 atcggcaccc gcctcggcct ctcgcggctg cgcctgcaga tcaacgagct gccgtccgac
     1201 agccactggc aggctctggc gaaggccgcg ctcggcgacg acctcgccgg gctgcagcgc
     1261 gcgctcgcgc aggacgtgct gcagcagggg ctgggcagcc ccgccgagtt gctggcgacc
     1321 tgggagacgg gcaacgcgat cgcgctcgag cgcgcgcagc ggctgctcgc cgagttggcc
     1381 gacgccaagg gaggcgccga cctatcgatg ctctcagtcg cgctgcggga gctgcgcaac
     1441 ctcggctagt ggaccctcgt tcgcctggct cggcaggctg ccggggtcac gtggccgagc
     1501 cgctgctaga ccttttgatt tgatccgaca agaagcggaa gatgcgttcg tcctcgctga
     1561 agcgacgttg aagcgaagcc tagagcgagt actcgtggtg caatccatcc aacacggatt
     1621 tcgctcgcac gagcgcaccc ggcgtccatg aatgtcggga ttcggaattc ggcgcgcgcg
     1681 cagatcccgc gggagggccg ggaacgccgg gtcccagcac gctctgcgga cgcccgccgt
     1741 t
//
LOCUS       DASYJA010000430         4802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1517028, whole genome shotgun sequence.
ACCESSION   DASYJA010000430 DASYJA010000000
VERSION     DASYJA010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517028"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /gene="glcE"
                     /locus_tag="VGO02_09405"
     CDS             complement(<1..893)
                     /gene="glcE"
                     /locus_tag="VGO02_09405"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HEV7477937.1"
                     /translation="MSLAALQERIREAAGRGTALRLRGGGSKDFYGNAPRGEILDTRA
                     FAGVVAYEPTELVVTARCGTSLLELERTLNEKNQFLPFEPPHFGDAATVGGCVAAGLS
                     GPRRAAAGALRDFLLGTKLIDGRGNLLVFGGQVMKNVAGYDVSRLLAGSLGTLGLIAE
                     ASIKVLPRPAVEATLRLRMTEERTLEALNRWAGQPFPVSATAWHDGDLSMRLSGAQAG
                     VRAAAAKLGGDELPPAQAAAFWQGIREHSDPFFAGEEPLWRIAVPPAASPLRLPARQM
                     IEWSGGLRWLKSTADPQVIREA"
     gene            complement(890..2377)
                     /locus_tag="VGO02_09410"
     CDS             complement(890..2377)
                     /locus_tag="VGO02_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEV7477938.1"
                     /translation="MGAALLARQREVADALRAFLPARAVLYEEEDTRPYECDGLTAYR
                     QLPMVVALPETEEQVQRVLKTCSSMKVPVVPRGAGTGLSGGALPPGDGVLLSMAKFMR
                     VLRVDPQSRVAVVQPGVRNAAISDVAAPFGLYYAPDPSSQIACTIGGNVSENSGGVHC
                     LKYGLTVHNVLRVRGYTAAGEPFEVGSEAPDAPGYDLLALAIGSEGMLMVVTEVTVKL
                     LPKPQSARVIMASFDDVERAGQAVADIIAAGIIPAGLEMMDRPAIHAVEQFVGAGYDL
                     AAAAILLCESDGGAEEVEEEIGRMKALLERSGATRMTVSRDEAERLKFWSGRKAAFPA
                     MGRISPDYYCMDGTIPRKRLGEVLTFIAGMEKKYGLRCPNVFHAGDGNLHPLILFDAN
                     SADELHRTEQFAAEVLEKCIAVGGTITGEHGVGVEKINQMCAQFGAGELEIFQGIKRA
                     FDPPGLLNPGKAVPTLARCGEYRSLRAGGGAGPGIAASHADLPRF"
     gene            complement(2341..3132)
                     /gene="otnI"
                     /locus_tag="VGO02_09415"
     CDS             complement(2341..3132)
                     /gene="otnI"
                     /locus_tag="VGO02_09415"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HEV7477939.1"
                     /translation="MPKLAANLSTLFTEVPFLERFVAAARAGFRAVEYQYPYEWPPRD
                     IAAAAGEAGVEVVLHNMPPGDAQLGERGTTCLTGREQRFREDLERAIEYARAARCKSL
                     HLMAGAVPTGAQRDALHATYVANLKYAAGRLGREGMRLLIEPLSERTVPNCFLRSSAQ
                     AAQVLEEVAAGNALIQYDLFHMQIMEGNLAATIERLLARIGHLQLADVPARNEPGTGE
                     INFEFLLGHIDRIGYSGWIGCEYNPRGDTLEGLKWAQPYLRGKGK"
     gene            3157..4017
                     /locus_tag="VGO02_09420"
     CDS             3157..4017
                     /locus_tag="VGO02_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV7477940.1"
                     /translation="MEAAAAHWWIWPLALFAMSFAIGIVAVLGGVGGGVLFVPIVGGF
                     FPFHLDFVRGAGLVFALAGTLAAGPTLLRNRMASLRLVMPLALAGSVASFVGAVVGLS
                     MSTELLQTALGLTVIVIAILIAASRDAFAAGAVESDALGAALRMKGDYEDPAAGARID
                     WRTHRTIAGFGAFGAIGFLGGLFGLGAGFANVPALNLLMAAPLKVAVGSSGLIISIIN
                     SSAAWVYIHRGALLPILAVPAILGVMLGSRIGARLLAVTPAATVRRVVVAVMLLAGTR
                     ALLRGLGLWT"
     gene            4008..4421
                     /locus_tag="VGO02_09425"
     CDS             4008..4421
                     /locus_tag="VGO02_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477941.1"
                     /translation="MDVREHDAEVQEIYARWLDLGTRAGFAASLVAFLVYVFGAVPPY
                     LPLDALPRYWGLPVGEFLRQTGAPSGWDWLRHLGGGAEYLNLACMALLGVVTVLCYMR
                     IVPVLLRLGERVQATLAIAQIAVLLAAASGFFAGG"
     gene            4475..>4802
                     /locus_tag="VGO02_09430"
     CDS             4475..>4802
                     /locus_tag="VGO02_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477942.1"
                     /translation="MGAIFQSLGRTITAGVVLLLVIMAIAGTYASVGNMDWWRFFFRW
                     LHVLSGVMWIGLLWYFNFVQTPSMPKIPDEQKPAVSKVIAPTALFWFRWGAMATIVTG
                     LILAWMN"
BASE COUNT          681 a         1644 c         1669 g          808 t
ORIGIN      
        1 gcttcgcgga tcacctgcgg gtcggcggtc gatttcagcc agcgcaggcc gccgctccac
       61 tcgatcatct gcctggccgg caggcgcaac ggcgaagcag ccggcggcac ggcgatgcgc
      121 cacagcggct cctcgccggc aaagaacgga tcggagtgct cgcggatgcc ttgccagaaa
      181 gcggcggcct gggccggcgg cagctcgtcg ccgccgagct ttgcggcggc agcgcgcacc
      241 ccggcttgcg cgccggacag gcgcatgctc aggtcgccgt cgtgccatgc agtcgccgag
      301 accgggaaag gctgtccggc ccagcgattg agcgcctcga gggtccgctc ctcggtcatc
      361 ctcagccgca acgtcgcctc gaccgccggc cgcggcagca ccttgatcga agcctcggcg
      421 atgaggccaa gggtgccgag cgagccggcg agcagacgcg aaacatcgta gccggcgacg
      481 ttcttcatca cctggccgcc gaagaccagc aggttgccgc ggccgtctat cagcttggtg
      541 cccagcagga agtcgcgcag cgcgcccgcg gctgcgcgtc gcgggccgga caatccggcc
      601 gccacgcagc caccgacggt cgcagcgtca ccgaagtgcg gcggctcgaa gggaagaaac
      661 tggttttttt cattcaatgt ccgttccagc tcgagaagcg aagtgccgca gcgcgcggtg
      721 accaccagct cggtcggctc gtaggcgacg acgccggcaa aggcgcgcgt gtcgaggatc
      781 tcgccgcgcg gcgcgttgcc gtagaagtcc ttgctgccgc cgccgcgcag gcgcagcgcg
      841 gtgccgcggc ccgccgcttc gcggatacgc tcctgtagcg ctgccaggct caaaagcgcg
      901 gcaaatcggc gtgagatgcg gcgatgcccg ggccggcgcc gccgccggcg cgcaggctcc
      961 gatactcgcc gcagcgcgcg agcgtcggca cggccttgcc gggattgagc aggcccggcg
     1021 gatcgaaggc gcgcttgatg ccctggaaga tctcgagctc gcccgcgccg aactgcgcgc
     1081 acatctggtt gatcttctcg accccgacgc cgtgctcgcc ggtgatggtg ccgcccaccg
     1141 cgatgcattt ctcgagcacc tcggcggcga actgctcggt gcggtgcagc tcgtcggcgc
     1201 tgttggcgtc gaacaggatc agcggatgca ggttgccgtc gccggcgtgg aacacgttcg
     1261 ggcagcgcag cccgtatttc ttctccatgc cggcgatgaa agtgagcacc tcgcccagcc
     1321 gcttgcgcgg gatggtgccg tccatgcagt agtagtcggg cgagatgcgc cccatcgccg
     1381 gaaaagccgc cttgcgcccg gaccagaact tcaggcgctc ggcctcgtcg cgcgacaccg
     1441 tcatgcgcgt cgcgccggag cgctccagca gcgccttcat gcggccgatt tcctcctcca
     1501 cttcctccgc gccgccgtcg gattcgcaca gcaggatcgc ggccgcggcg aggtcgtagc
     1561 cggcgccgac gaactgctcg acggcgtgga tggcgggccg gtccatcatc tccaggcccg
     1621 ccgggatgat gccggcggcg atgatatcgg cgacggcctg cccggcgcgt tcgacgtcgt
     1681 cgaacgaggc catgatcacc cgcgcgcttt gcggcttggg cagcagcttg acggtcacct
     1741 cggtgaccac catcagcatg ccttccgagc cgatggcgag cgcgagcagg tcgtagcccg
     1801 gcgcgtccgg cgcttcggag ccgacctcga acggctcgcc ggcggcggtg tagccgcgca
     1861 cgcgcagcac gttgtgcacc gtcaggccgt acttgaggca gtgcaccccg cccgaattct
     1921 ccgagacgtt gccgccgatg gtgcaggcga tctggctgga agggtcgggc gcgtagtaca
     1981 gcccgaacgg ggccgcgacg tcggagatcg cggcgttgcg cacgccgggc tgcactaccg
     2041 cgacgcggct ctgcggatcg acgcgcagca cgcgcatgaa cttcgccatg gaaaggagca
     2101 cgccgtcgcc cggcggcagc gccccgccgg agaggccggt cccggcgccg cggggtacca
     2161 cgggaacctt catggaagaa caggttttca gtacgcgctg cacctgctct tcagtttccg
     2221 gcagcgcgac caccatcggc agctgccggt aggcagtcag gccgtcgcat tcgtacggcc
     2281 gcgtgtcctc ttcctcgtag agcacggcgc gcgccggcag aaaggcgcgc agggcgtcgg
     2341 ctacttccct ttgcctcgca agtaaggctg cgcccacttc aggccctcga gcgtgtcacc
     2401 ccggggattg tattcgcaac cgatccagcc ggaatatccg attcggtcga tgtgaccgag
     2461 caggaactcg aagttgatct cccccgtgcc gggctcgttg cgcgcgggca cgtcggcgag
     2521 ctgcaggtgc ccgatgcgcg ccagcaggcg ctcgatggtc gcggcgaggt tgccttccat
     2581 gatctgcatg tggaacaggt cgtactggat gagcgcattg ccggcggcca cctcttccag
     2641 cacctgcgcg gcctgcgcgc tcgagcgcag gaagcaattg ggcaccgtgc gctcggagag
     2701 cggctcgatc agcaggcgca tgccttcgcg acccaggcgc ccagcggcgt acttgaggtt
     2761 ggcgacatac gtcgcgtgca gggcgtcacg ctgcgcgccg gtggggacgg cgcccgccat
     2821 caggtgaagg ctcttgcagc gcgccgcgcg cgcgtactcg atcgcgcgct cgagatcttc
     2881 gcggaagcgc tgctcgcgtc cggtcaggca ggtcgtgccg cgctcgccga gctgtgcatc
     2941 gcctggcggc atgttgtgca gcaccacctc gacgccggct tcgccggcgg cggccgcgat
     3001 gtcccgcggc ggccactcgt acggatactg gtactcgacg gcgcgaaacc cggcgcgcgc
     3061 cgcggcgacg aaacgctcca ggaacggcac ttcggtgaac agcgtcgaga ggttggcggc
     3121 gagcttcggc ataatgcgag cataagctga aaacacatgg aagccgctgc cgcccactgg
     3181 tggatctggc cgctcgcgct gttcgcgatg agcttcgcca tcggcatcgt ggcggtgctc
     3241 ggcggcgtcg gcggcggcgt gctgttcgtg ccgatcgtcg gcggcttctt tccgttccac
     3301 ctcgacttcg tgcgcggcgc cggcctggta ttcgcgctcg ccgggacgct tgccgccggc
     3361 ccgacgctgc tgcgcaatcg catggccagc ctgcgcctgg tgatgccgct cgccctcgcc
     3421 ggctcggtgg cgtcgttcgt cggcgccgtg gtcggacttt cgatgtccac cgagctcctg
     3481 cagaccgcgc tcggcctgac ggtgatcgtg atcgccatcc tgattgccgc ctcgagggac
     3541 gccttcgccg ccggcgcggt cgagtccgac gcgctcggcg cggcgttgcg catgaaaggc
     3601 gactacgagg atccggccgc cggcgcgcgc atcgactggc gcacccaccg caccatcgcg
     3661 ggattcggcg ccttcggcgc gatcggcttc ctgggcgggc tgttcggcct gggcgccggc
     3721 ttcgccaacg tgccggcgct caatctgctc atggcggcgc cgctcaaggt cgcggtcggc
     3781 tccagcgggc tgatcatttc catcatcaac agctccgcgg cctgggtgta catccaccgc
     3841 ggcgcgctgc taccgatcct cgccgtgccg gcgatcctcg gcgtcatgct cggctcgcgc
     3901 atcggtgccc ggctgctggc ggtgacgccg gcggccacgg tgcgacgcgt ggtggtcgcg
     3961 gtgatgttgc tcgccgggac aagagcgctg ctgaggggat tgggcttatg gacgtgagag
     4021 agcacgacgc cgaggtgcag gaaatctatg cgcgctggct cgatctgggc acgcgcgcgg
     4081 gcttcgcggc gtcgctcgtg gcctttcttg tctatgtgtt cggcgcggtg ccaccctatc
     4141 tgccgctcga tgcgctgccg cgctattggg ggctgccggt aggtgaattc ctccgccaga
     4201 ccggggcgcc ctccggctgg gactggctca ggcatctcgg cggtggcgct gagtatctca
     4261 acctcgcttg catggcgctg ctgggggtag tcacggtgct gtgctatatg cgaatcgttc
     4321 ctgttctgct ccggctgggc gagcgcgtgc aggccaccct cgcgatcgcg cagatcgccg
     4381 tcctgctggc ggccgcctcg ggatttttcg ccggcggttg acgcgtgcta gacttttttc
     4441 gccgcaaacc caccacacaa agagggagaa acccatgggt gccatcttcc aatctctagg
     4501 tcggaccatc actgccggcg tcgtgctcct gctggtaatc atggccatcg ccggaaccta
     4561 cgcgagcgtc ggcaacatgg actggtggag gttcttcttc cgctggctgc acgtgctgtc
     4621 gggggtgatg tggatcgggc tgctgtggta cttcaacttc gtgcagacgc cctcgatgcc
     4681 caagatcccg gacgagcaga agccggcggt aagcaaggtg atcgcgccga cggcgctgtt
     4741 ctggttccgc tggggcgcca tggcgaccat cgtcacgggc ctgatcctcg cctggatgaa
     4801 cg
//
LOCUS       DASYJA010000431         3700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_336909, whole genome shotgun sequence.
ACCESSION   DASYJA010000431 DASYJA010000000
VERSION     DASYJA010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336909"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VGO02_09435"
     CDS             <1..203
                     /locus_tag="VGO02_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477943.1"
                     /translation="RVPRRRHRRYLAASVAAAILLVGAGVGARISAEGQDRFLAGMHA
                     KYRAATAQGKSYMEALVRKAKF"
     gene            203..1354
                     /locus_tag="VGO02_09440"
     CDS             203..1354
                     /locus_tag="VGO02_09440"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV7477944.1"
                     /translation="MAKKKLARVGRYKILGELGRGAMGIVYKAEDPNLDRVVALKTIL
                     LSDDAEGRKDYHKRFFLEAKAAGKLTHPHIVTTYDFGEEDDLAYLAMELLEGDDLRTR
                     MKEGPLAIADSVDITQQVADGLGFAHERGVVHRDIKPGNIMLLPRGQAKIMDFGIARM
                     RSDDFKTSTGIVMGTPRFMSPEQVAGDPVDQRSDIFSLGVVLYEMLTGTALFAGEDTP
                     QIAHNVANVEHVPPSRLNPEVPSMLDFAVARALKKDPAVRYQDAYEFAVDLRTCLAEL
                     RSRETAAKRDSNGGTKTLRLDSTGDRAPLAPAARSIASDTRLPLSRQFDSFAAVQRLT
                     AQSRRDRKVLAKPPRPVKFLRRVFRDAGPRLLFLTSLVAAAAGVYVGFG"
     gene            complement(1351..3528)
                     /locus_tag="VGO02_09445"
     CDS             complement(1351..3528)
                     /locus_tag="VGO02_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV7477945.1"
                     /translation="MSSRRDFLKGSGALVLLFSFNHSYAQGGPARLPGSLNGNRMLSG
                     WLRIDADGTVTIFSGKIELGQGIGTALAQIAAEELDVDLKRVSIVHGDTALTPNEGQT
                     AGSQSVQDSGTAVRFACAEARAMLLTAAAAKLGTNELTVVDGTINGSLTYWTLASELD
                     LKKEATGSAKPKPAAEHKLVGKSVPRRDIPKKFTGGAAYVQDIRLPRMVFGRVVRPPS
                     PGATLISLDENQIKKMPGVVAVVRDGNFLAVAAEREEQAIKARAALGKSAVWKESATL
                     PPSGDALYEHMVGLNVPAQVMLDKSGAAAPAARKLEARYTRPFQAHGSIGPSCAVAVW
                     ENEKKLHVWTHSQGVYPLRGDLANVFGLEPSAVRCTHAEGAGCYGHNGADDVALDAAL
                     LARATSGRPVKLQWMREDEFMWEPYGSAMVMRLGGALDAQGNIVSWSHELWSHPHSRR
                     PGQSKGSHTLAARHLARPVGAEPVGDVPLPAGGSDRNAIPGAYDIPNVKVSKHFIIES
                     PLRTSALRTLGGYGNVFALESFMDELAAAAGADPVEFRLRHVKDPRARAVIEATATRA
                     GWRSNPSNWGFAYARYKNVGCYCAVAAEVAVDPKTGQVLVKRAVSAVDVGQAVNPDGI
                     VNQIEGGIIQSASWTLKEAVSFDRTRVRTRSWADYPILRFDEVPQVDVVILNQPDQPF
                     LGVGEGSQGPAAAAIANALASATGKRLRDLPFTPARVKATLAA"
     gene            complement(3525..>3700)
                     /locus_tag="VGO02_09450"
     CDS             complement(3525..>3700)
                     /locus_tag="VGO02_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012217239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEV7477946.1"
                     /translation="CGYCGNGMVMSAKALLARNPRPTNEEIRKALNGHLCRCASHNRI
                     VRAVQRAAKEMQS"
BASE COUNT          553 a         1313 c         1235 g          599 t
ORIGIN      
        1 atcgtgtacc ccgccggcgc caccgtcggt atctggcggc cagcgtggcg gcggcgatcc
       61 tgctcgtggg cgccggcgtc ggcgcgcgca tctccgccga gggccaggac cgcttcctcg
      121 ccggcatgca cgccaagtac cgcgccgcca ccgcgcaggg gaagagctac atggaggcgc
      181 tggtgcgcaa ggcgaagttc tgatggcgaa gaagaagctc gcccgcgtcg ggcgctacaa
      241 gatcctcggc gagctcggcc gcggcgcgat gggcatcgtg tacaaggccg aggacccgaa
      301 cctcgaccgc gtggtggcgc tgaagaccat cctgctgtcc gacgacgccg aggggcgcaa
      361 ggactaccac aagcgcttct tcctcgaggc caaggccgcc ggcaagctga cccacccgca
      421 catcgtcacc acctacgact tcggcgagga ggacgacctc gcttacctcg cgatggagct
      481 gctcgagggc gacgacctgc gcacgcggat gaaggagggg ccgctcgcca tcgccgactc
      541 ggtcgacatc acgcagcagg tggccgacgg cctgggcttc gcgcacgagc gcggcgtcgt
      601 gcaccgcgac atcaagcccg gcaacatcat gctgctgccg cgcggccagg cgaagatcat
      661 ggacttcggc atcgcgcgca tgcgcagcga cgacttcaag accagcaccg gcatcgtcat
      721 gggcacgccg cgcttcatgt cgcccgagca ggtggcgggc gacccggtgg accagcgctc
      781 cgacatcttc tccctcggcg tggtgctcta cgagatgctc accggcaccg ccctcttcgc
      841 cggcgaggac acgccgcaga tcgcgcacaa cgtggccaac gtcgagcacg tgccgccctc
      901 gcgcctcaac cccgaggtgc cttcgatgct cgatttcgcg gtggcgcgcg cgctgaagaa
      961 ggacccggcg gtgcgctacc aggatgccta cgagtttgcc gtcgacctgc gcacctgcct
     1021 ggccgagctg cgctcgcgcg agaccgcggc gaagagggac agcaacggcg gcaccaagac
     1081 cctcaggctc gattcgaccg gcgaccgcgc gccgctcgcg ccggcggcgc gctcgatcgc
     1141 ctccgacacg cggctgccgc tgtcgcgcca gttcgattcc ttcgccgccg tgcagcgcct
     1201 gacggcgcag agccggcgcg accgcaaggt gctcgccaag ccgccgcggc cggtgaaatt
     1261 cctgcgccgc gtcttccgcg acgccggtcc gcgcctgctc ttcctgacga gcctcgtcgc
     1321 cgctgctgcc ggcgtttacg tcggtttcgg ctaggcggcg agcgtcgcct tcacgcgagc
     1381 gggcgtgaag ggaaggtcgc gcagccgttt cccggtggcg ctggccaggg cattggcgat
     1441 cgccgccgcc gcaggtccct gcgagccttc gcccacgccg agaaacggct ggtccggctg
     1501 gttcaggatc accacgtcga cctgcggcac ttcgtcgaag cgcaggatcg ggtagtcggc
     1561 ccaggagcgc gtcctgacgc gcgtgcggtc gaagctgacc gcttccttga gcgtccagct
     1621 cgccgactgg atgatgccgc cttcgatctg gttgacgatg ccgtccgggt tgaccgcctg
     1681 ccccacgtcg acggccgaga cggcgcgctt taccagcact tgtccggtct tgggatccac
     1741 cgcgacctcg gcagcaacgg cgcaatagca gccaacgttc ttatatctgg cataagcgaa
     1801 accccaatta cttgggtttg atcgccatcc ggcgcgcgtc gcggtggcct cgatcacggc
     1861 gcgggcgcgc ggatccttta catgccgcag gcggaattcc accgggtcgg cgccggccgc
     1921 cgccgccagc tcgtccatga acgattcgag cgcgaagacg ttgccgtagc cgccgagggt
     1981 gcgcagggcc gaggtgcgca gcggcgattc gatgatgaag tgcttggata cctttacgtt
     2041 cgggatgtcg tacgcccccg ggatcgcgtt gcggtcggag ccgcccgccg gcagcggcac
     2101 gtcgcccacc ggctcggcgc cgaccggtct tgcgagatgg cgcgccgcca gcgtgtgcga
     2161 gcccttcgac tggcccggac gccggctgtg cggatggctc cacagctcgt gcgaccagga
     2221 gacgatgttg ccctgcgcgt cgagcgcgcc gcccaggcgc atcaccatcg ccgagccgta
     2281 cggctcccac atgaactcgt cctcgcgcat ccactgcagc ttgaccggcc ggccggaggt
     2341 ggcgcgcgcg agcagcgccg catcgagcgc gacgtcgtcg gcgccgttgt gcccgtagca
     2401 gccagcccct tcggcgtgcg tgcagcgcac cgcggatggc tcgaggccga acacgttggc
     2461 gaggtcgccg cgcaaggggt agaccccctg gctatgcgtc cacacgtgta attttttttc
     2521 attttcccaa acagcgacag cgcacgaagg cccaatggag ccgtgcgcct ggaaggggcg
     2581 cgtatagcgc gcttcgagct tcctggccgc cggcgccgcg gcgccggatt tatcgagcat
     2641 cacctgcgcg ggcacgttca ggccgaccat gtgctcgtag agcgcatcgc cggagggcgg
     2701 cagcgtggcg ctttccttcc acaccgcgct tttgccgagc gccgcgcgag ccttgatcgc
     2761 ctgctcctcg cgctcggcgg caacggccag gaaatttcca tcccgcacca ccgcaacgac
     2821 cccgggcatt ttcttaattt gattttcatc cagagagatc agagttgcgc ccggcgaagg
     2881 cgggcgcacg acgcggccga acaccatgcg cggcaggcgg atgtcctgca cgtaggccgc
     2941 cccgccggtg aatttcttcg ggatgtcgcg ccgcggcacg ctcttgccga ccagcttgtg
     3001 ctcggccgcg ggcttgggct tcgcgctgcc ggtggcttcc ttcttcaggt cgagctcgct
     3061 cgccagagtc caataggtca gggagccgtt gatggtccca tcgacgaccg tcagttcatt
     3121 cgtgccgagc ttcgccgccg cggcggtgag aagcatcgcg cgcgcttcgg cgcaggcgaa
     3181 gcgcaccgcg gtgccgctgt cctgcaccga ctggctgccc gcggtctgcc cttcgttcgg
     3241 cgtcagcgcc gtgtcgccgt gcacgatgga cacgcgcttc aggtcgacgt cgagctcctc
     3301 ggcggcgatc tgcgcgagcg cggtgccgat gccctggccg agctcgatct tgccgctgaa
     3361 aatcgtcacc gtgccgtcgg cgtcgatgcg cagccagccg ctcagcatgc ggttgccgtt
     3421 caggctgccc ggtagcctcg ccggtccgcc ttgtgcatat gaatgattga atgaaaacag
     3481 caggaccagc gcaccgctgc ccttcaggaa atcgcgccgc gagctcatga ctgcatctcc
     3541 ttcgccgcgc gctgcaccgc ccgcacgatg cggttatgcg aggcgcagcg gcacaggtgg
     3601 ccgttgagcg ccttgcggat ctcctcgttg gtcggccgcg ggttgcgcgc gagcagcgcc
     3661 ttcgccgaca tcaccatgcc gttgccgcag tagccgcact
//
LOCUS       DASYJA010000432         1513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1881524, whole genome shotgun sequence.
ACCESSION   DASYJA010000432 DASYJA010000000
VERSION     DASYJA010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881524"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..1142
                     /locus_tag="VGO02_09455"
     CDS             84..1142
                     /locus_tag="VGO02_09455"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEV7477947.1"
                     /translation="MSAPSHISIEQEELRGISRTVAEIHWLLLILVLLFLVFGGERDD
                     AEASAAVAAGLFFYAAMVMSFRYANFYRRETRWKIAIETWGMIAFLTWTLRFTDGLGS
                     PLVNAYLLPVITAALTLGKITTLGIVGLIAACYIYLGDVSAPELASLRFVAGFAAQLA
                     PVLLVAYITTMFSADIRYGLQRAKLLSETDDLTGMFNTRGFAIAANRLFAQAVRHNRS
                     ASVLMIDSDNLKAVNDSHGHDAGNRLLRHLASSIQAELRFTDVAARYGGDEFIVLLPE
                     TPSKGAFEVAERIRNAITARPLEMGERRIVSSVSIGIATFPEDGASLDSLAAHADRAM
                     YAAKEAGRNRSMKFRAVA"
     gene            complement(1139..>1513)
                     /locus_tag="VGO02_09460"
     CDS             complement(1139..>1513)
                     /locus_tag="VGO02_09460"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7477948.1"
                     /translation="AKFIKRFDAARERCWIAQMDGEPVGSVFLVKETNTIAKLRLLLV
                     EPRARGQGLGRRLVRECIAFARARGYRKLALWTQSNLAAARAIYKAEGFTLAGTEKHA
                     SFGVKLTGEYWELNLKGRELKL"
BASE COUNT          241 a          549 c          472 g          251 t
ORIGIN      
        1 ggcggcgccc gcggtgacga gcacgcgcaa tccattggcg gacaattcca tggcgttgga
       61 taatacggca agaacacgcg cccatgtccg ccccaagcca catctccatc gaacaggaag
      121 agctgcgcgg catctcgcgc accgtcgccg agatccactg gctgctgctg atcctggtgt
      181 tgctgttcct ggtgttcggt ggcgagcgcg acgacgccga ggcctccgcg gcggtcgccg
      241 ccgggctgtt cttctacgcc gcgatggtga tgagcttccg ctacgcgaac ttctaccggc
      301 gcgagacgcg ctggaagatc gccatcgaga cctgggggat gatcgccttc ctcacctgga
      361 cgctgcgctt caccgacggc ctgggcagcc cgctggtcaa cgcctacctg ctgccggtga
      421 tcaccgcggc gctgacgctg ggcaagataa ccacgctcgg catcgtcggg ctgatcgccg
      481 cgtgctacat ctacctcggc gatgtctcgg caccggagct cgcctcgctg cgcttcgtgg
      541 cggggttcgc cgcgcagctc gcgccggtgc tgctggtcgc ctacatcacc accatgttct
      601 cggccgacat ccgctacggc ctgcagcgcg ccaagctgct ctccgagacc gacgacctta
      661 ccggcatgtt caacacgcgc ggcttcgcca tcgcggcgaa ccgcctgttc gcccaggcgg
      721 tgcgccacaa ccgctcggcc agcgtgctga tgatcgactc cgacaacctc aaggcggtga
      781 acgacagcca cggccacgac gccggcaacc ggctgctgcg ccatctggcg agctcgatcc
      841 aggccgagct gcgcttcacc gacgtcgccg cgcgctacgg cggcgacgag ttcatcgtgc
      901 tgctgcccga gacgccgtcc aaaggcgcct tcgaggtcgc cgagcgcatc cgcaacgcca
      961 tcaccgcgcg gccgctcgag atgggtgagc ggcgcatcgt ctcgagcgtg agcatcggca
     1021 tcgccacctt ccccgaggac ggcgccagcc tcgactcgct cgccgcccac gccgaccgcg
     1081 cgatgtacgc ggcgaaagag gccgggcgca accgctcgat gaagttccgc gccgtcgcct
     1141 agagcttcaa ttccctgcct tttagattca attcccaata ctcgccggtg agcttcacgc
     1201 cgaagctggc gtgcttctcg gtgcctgcca gcgtgaagcc ctccgccttg tagatggcgc
     1261 gcgccgcggc gaggttggac tgcgtccaca gcgcgagctt gcgatagccc ctggcgcgcg
     1321 cgaaggcgat gcattcgcgc accaggcgcc ggccgagccc ctggccgcgc gcccgcggct
     1381 cgaccagaag cagccgcagt ttcgcaatgg tgtttgtttc cttgaccaga aagacggaac
     1441 caacgggctc accgtccatc tgcgcgatcc agcagcgctc gcgcgcggcg tcgaaccgct
     1501 tgatgaactt cgc
//
LOCUS       DASYJA010000433         1846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1154606, whole genome shotgun sequence.
ACCESSION   DASYJA010000433 DASYJA010000000
VERSION     DASYJA010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154606"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..634)
                     /gene="apaG"
                     /locus_tag="VGO02_09465"
     CDS             complement(224..634)
                     /gene="apaG"
                     /locus_tag="VGO02_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEV7477949.1"
                     /translation="MAARVYNAGVAQEKQYRVAVSATTQFIAEQSDEAAGRYVFAYTI
                     TIRNGGNVAAQLISRHWVITDAQGLVQEVRGLGVVGAQPLLQPGETYEYTSGTSIGTQ
                     VGTMRGSYQMLAADGTRFEAPIPEFTLSVPRVLH"
     gene            633..1298
                     /gene="rpe"
                     /locus_tag="VGO02_09470"
     CDS             633..1298
                     /gene="rpe"
                     /locus_tag="VGO02_09470"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV7477950.1"
                     /translation="MTFRIAPSILSADFARLGEEVRAVDQAGADLIHFDVMDNHYVPN
                     LTVGPLVCAALRPHVTVPLDVHLMVRPVDRLVADFAKAGANIISFHPEASDHVDRTIA
                     LIKENGCKVGLVLNPATPLAWLDCTLEKLDLVLLMSVNPGFGGQQFIASVLPKIEETR
                     RRIRATGKPIWLEVDGGIKADNIAQVARAGADTFVAGSAIFGAKDYAKAIAGMRAELS
                     KVL"
     gene            1426..>1846
                     /locus_tag="VGO02_09475"
     CDS             1426..>1846
                     /locus_tag="VGO02_09475"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:NF016593.4"
                     /note="with component II, the glutamine amidotransferase,
                     catalyzes the formation of anthranilate from chorismate
                     and glutamine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEV7477951.1"
                     /translation="MTETQFLELAAAGYRRIPVLAEARADLYTPLAVYVTLAGGPYSY
                     LLESVVGGERFGRYSFIGLGCAERIEVRGGGIRRLLRDARGADVCLESVEGDPFEYAR
                     AWLKRHRTAPVPAALRFGGGLVGYFGYETVQHVEKNCL"
BASE COUNT          278 a          615 c          623 g          330 t
ORIGIN      
        1 cgagcgtggc gcggctccac cccggcagcg cctcgaagga catcgccagg tagcgcgcgg
       61 tttcgggcgt cggcggcttg ggctgcggcg gcgtctgcgc gcagccggcc accattagaa
      121 tcagcgcgat gtgggtagtc ttcatcgaaa tcggggtcgc gctcttcctg gccgtgttca
      181 tcgtctggtt cacctggccg aaaaagaaag aggaagacaa agatcagtga agcacgcgcg
      241 gcactgacag cgtgaactcc gggatcggcg cctcgaagcg ggtgccgtcc gcggcgagca
      301 tctggtagct gccgcgcatg gtgccgacct gggtgccgat cgaggtgccg ctggtgtact
      361 cgtaggtctc gcccggctgc agcagcggct gcgcgccgac cacgcccagg ccgcgcactt
      421 cctgcaccag gccctgggca tcggtgatca cccaatgccg gctgatcaac tgcgccgcga
      481 cgttgccgcc gttgcggatc gtgatggtgt aggcgaagac gtagcgtccg gccgcctcgt
      541 cggactgctc cgcgatgaat tgcgtcgtcg cactgactgc gacacggtat tgcttctcct
      601 gcgccacgcc tgcattgtaa actcgcgcgg ccatgacgtt ccgcatcgcc cccagcatcc
      661 tgtccgccga cttcgcgcgg ctgggcgaag aggtgcgcgc ggtggatcaa gccggggcgg
      721 atctgatcca tttcgacgtg atggacaacc actacgtccc caacctcacc gtcgggccgc
      781 tggtgtgcgc ggcgctgcgg ccgcacgtga cggtgccgct cgatgtccac ctgatggtgc
      841 ggccggtcga ccgcctggtg gccgatttcg ccaaggcggg ggcgaacatc atcagcttcc
      901 atcccgaagc ctccgatcac gtggaccgca ccatcgcgct gatcaaggag aacggctgca
      961 aggtgggcct ggtgctcaac ccggcgacgc cgctggcgtg gctggattgc acgctggaga
     1021 agctcgatct ggtgctgctg atgtcggtca accccggctt cggcgggcag cagttcattg
     1081 cctcggtgct gccgaagatc gaggagacgc ggcgtcgcat ccgggccacc ggcaagccga
     1141 tctggctcga ggtcgacggc ggcatcaagg ccgacaacat cgcgcaagtg gcgcgcgccg
     1201 gcgcggatac cttcgtcgcc ggctcggcga tattcggcgc caaggactac gccaaggcca
     1261 tcgccgggat gcgtgccgag ctttcgaagg tgctatagtc tgcagcgtcg tgtttttcct
     1321 ccccgcacaa ctcgtctccc tccactggcg tagctggcgc cgctagttcc gcacgtcgtt
     1381 tcgccgtagt tccccagcag tagccgttgc cgtacggaga gagttgtgac cgaaacccag
     1441 tttctcgagc tcgcagccgc gggctaccgc cgcatcccgg tgctcgccga ggcgcgcgcc
     1501 gacctctaca cgccgctcgc ggtctacgtc accctggccg gcggacccta ttcttacctg
     1561 ctggagtcgg tcgtcggcgg cgagcgcttc gggcgctact cgttcatcgg tctgggctgc
     1621 gccgagcgca tcgaggtacg cgggggtggc atccggcggc tgctgcgcga cgcccgaggc
     1681 gccgacgtgt gcctcgaatc ggtcgaaggc gatccgttcg aatatgcccg cgcctggctg
     1741 aagcgccatc gcaccgcgcc ggttccggcg gcgctgcgct tcggtggcgg gctggtcggc
     1801 tatttcggct acgagaccgt gcagcacgtc gagaagaatt gcctga
//
LOCUS       DASYJA010000434         2737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2516501, whole genome shotgun sequence.
ACCESSION   DASYJA010000434 DASYJA010000000
VERSION     DASYJA010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2516501"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..161
                     /locus_tag="VGO02_09480"
     CDS             <1..161
                     /locus_tag="VGO02_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEV7477952.1"
                     /translation="WYTFDPRNNKPRVGRVLIARGRDAADVAISSTFGPNTLTSFRVW
                     TDEVRAGN"
     gene            212..844
                     /locus_tag="VGO02_09485"
     CDS             212..844
                     /locus_tag="VGO02_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477953.1"
                     /translation="MRQSVGFTLLLAMLWGCSHTELVSVPPKMSLKSYGTLGIVDFAS
                     NSDPAINSRATREFQAQIHAAQPGTRLVELGSRETLLAAAGGRQLDVEALRKIAAKYG
                     VDAIFLGDITYSDPVTNVKIGDLSKLEGGMHTEVRGDMSARLVETGTGASVWSSSSWA
                     RRQVNRLSISSEQGISGSTGNANPQAEMLPALIGHLTSDFRPTSTRQQVK"
     gene            complement(871..1623)
                     /locus_tag="VGO02_09490"
     CDS             complement(871..1623)
                     /locus_tag="VGO02_09490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7477954.1"
                     /translation="MAQKRVALVTGAGSGIGKSAALALIKDGYHVALVGRRKDMLEKT
                     AAESGAKDRALVLPGDITQDSSVKEIFSKVKAAWGRLDVLFNNAGMGAPAVPMEDLPI
                     EKFREVVNINLVAMFMCTQEAIRIMKAQDPKGGRIINNGSISAHAPRPMSVAYTSTKH
                     AVTGLTKCISLDGRKNDITASQIDIGNALTDLAARMANGVPQADGSVKPEPLMDVKHV
                     GDAVLHMANLPLDVNVQFMTIMATKMPLVGRG"
     gene            complement(1551..2396)
                     /locus_tag="VGO02_09495"
     CDS             complement(1551..2396)
                     /locus_tag="VGO02_09495"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7477955.1"
                     /translation="METVFVGPAPRIALSVHGNGPLVLFLHGIGGNRHHWREQLPIFA
                     TRYKAAAWDARGYGESDDYAGPLDFGSFTQDLLRVLDFFKAQKAHLVGLSLGGRIARN
                     FALRHPQRVARLVLANTTPGFSAFTPQELKKFVEERRNRTPESIRALISRHARPGAYE
                     ELLASFKALRQPSYLKTLEASVAQDRAAPIEKIRVPTLVITSDEDKVYPPSIARAMAK
                     RIPGAQLAVISGAGHLSNLEQPGQFNQVVLRFLSAQEKQPWHKKESRSSRALAAGSAS
                     PRRSR"
     gene            complement(2398..>2737)
                     /locus_tag="VGO02_09500"
     CDS             complement(2398..>2737)
                     /locus_tag="VGO02_09500"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEV7477956.1"
                     /translation="LKAGRLKAIAIGGPRRSPLLPDVPTFTELGFPEVETHAWFGLFL
                     PAGSPREAVSRIYSDTKRILDEPEFRQKQLIDRGYEPIGSSPDVFSDFIKTDKENRGR
                     AVKISGAKAE"
BASE COUNT          439 a          888 c          934 g          476 t
ORIGIN      
        1 gctggtacac gttcgacccg cgcaacaata agccgcgcgt cggcagggtc ctcattgcgc
       61 gcggccggga cgccgccgac gtcgcgatca gcagcacctt cggccccaac accctcacca
      121 gcttcagggt ctggaccgac gaggtgcgcg ccgggaattg aagctcgccg gccgggttac
      181 tatgcgtcca gactagaaag ggaggtgtgc aatgcgacag tccgttggat ttacgctgct
      241 gcttgccatg ctgtggggat gctcgcacac agagctcgtc tccgtgccgc cgaagatgag
      301 cctgaagagt tacgggacgc tcggcatcgt ggatttcgct tccaattccg atccggcgat
      361 caattcgcgc gccacgcggg agttccaggc gcagatccac gccgcccagc cggggacgcg
      421 cctggtggag ctggggagcc gcgagacgct gctcgcggcg gcgggcggcc ggcagctcga
      481 cgtcgaggcg ctgcgcaaga tcgccgcaaa gtacggcgtg gacgcgatct tcctcggcga
      541 cattacctat tccgacccgg tcaccaacgt caagatcggc gacctgtcca agctggaggg
      601 cggcatgcac accgaggtgc ggggcgacat gtccgcaagg ctggtcgaga ccggcacggg
      661 ggcgagcgtg tggagcagct cgtcatgggc gcggcggcag gtcaaccgcc tcagcatctc
      721 gtcggagcag ggcatcagcg gctcgacggg caacgccaac ccgcaggcgg agatgctgcc
      781 ggcgctcatc gggcacctga cgagcgattt caggccgact tcgacgcgcc agcaggtgaa
      841 atagctagcc gcgcccgacc gcgggtgggc ttatccccgc cctacgagcg gcatcttggt
      901 ggccatgatg gtcatgaact gcacgttgac gtcgagcggc aggttggcca tgtgcaggac
      961 cgcgtcgccg acgtgcttca cgtccatcag cggctcgggc ttgaccgagc cgtcggcctg
     1021 cggcacgccg ttggccatgc gcgcggcgag gtcggtgagg gcgttgccga tgtcgatctg
     1081 gctggcggtg atgtcgttct tgcggccgtc gagcgagatg cacttggtca ggccagtcac
     1141 tgcgtgcttg gtggaggtgt aggccaccga catcggccgc ggcgcgtgcg cggagatgga
     1201 gccgttgttg atgatgcgcc cgcccttggg gtcctgcgcc ttcatgatgc ggatcgcctc
     1261 ctgcgtgcac atgaacatgg cgaccaggtt tatgttgacc acctcgcgga acttctcgat
     1321 cggcaggtct tccatcggca ccgccggcgc gcccatgccg gcgttgttga acagcacgtc
     1381 gaggcggccc cacgccgcct tcactttcga gaaaatctct tttacggagg aatcctgagt
     1441 gatatcgccg ggcaacacga gcgcgcggtc cttggcgcca gactcggcgg cggtcttttc
     1501 cagcatgtcc ttgcggcggc cgaccagcgc gacgtggtag ccgtccttga tcagcgcgag
     1561 cgccgcggac ttgccgatcc cgctgccagc gcccgtgacg agcgcgactc ttttttgtgc
     1621 catggctgct tctcctgtgc cgaaagaaac cgcaagacta cttgattgaa ctggcccggc
     1681 tgctccaggt tcgaaaggtg cccggcgccg ctgatcacgg cgagctgcgc gccgggtatg
     1741 cgcttcgcca tggcgcgcgc gatcgaaggc gggtacacct tgtcctcgtc gctcgtgatg
     1801 accagcgtcg gcacgcggat cttctcgatc ggcgccgcgc ggtcctgcgc caccgaggcc
     1861 tccagcgtct tcaggtacga cggctggcgc aacgccttga agctcgcgag gagctcttcg
     1921 taggcgcccg ggcgggcatg ccggctgatc agcgccctga tcgactccgg cgtgcggttc
     1981 ctgcgctctt cgacgaactt cttcagctct tgcggcgtga acgcgctgaa gcccggcgtg
     2041 gtgttggcga gcaccaggcg cgcaacgcgc tgcgggtggc gcagggcgaa gttgcgcgcg
     2101 atgcggccgc ccagcgacag gccgaccagg tgggccttct gggccttgaa gaagtcgagg
     2161 acgcgcaaca gatcctgcgt gaaggagccg aagtcgagcg gccctgcata gtcgtccgac
     2221 tcgccgtagc cgcgcgcgtc ccacgccgcc gccttgtagc gcgtggcgaa gatcggcagc
     2281 tgctcgcgcc aatggtgccg gttgccgccg atgccgtgca ggaacagcac cagcggccca
     2341 ttgccgtgga cgctcagcgc gatgcggggg gcggggccga cgaagacggt ttccacgcta
     2401 ctccgccttg gcgcccgaga ttttcactgc gcgtccacga ttttccttat ccgtttttat
     2461 gaagtcggaa aaaacatcgg gcgagctgcc gatgggctcg tagccgcgat cgatgagctg
     2521 cttctggcgg aactcgggct catcgaggat gcgcttggta tcggaataga tgcgcgacac
     2581 cgcctccctc ggcgagcccg ccggcaggaa caggccgaac caggcgtgcg tctcgacttc
     2641 cgggaaaccc agttccgtga aggtcggcac atcgggcagc agcggcgagc gcctcggccc
     2701 gccgatggcg atcgccttca accggcccgc cttgagc
//
LOCUS       DASYJA010000435         2290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2698931, whole genome shotgun sequence.
ACCESSION   DASYJA010000435 DASYJA010000000
VERSION     DASYJA010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2698931"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VGO02_09505"
     CDS             complement(<1..506)
                     /locus_tag="VGO02_09505"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV7477957.1"
                     /translation="MAEPWLPLAGLRVVDFSMFVPGPFCSALLADLGAEVIKVEQPKG
                     DPGRSYVPVQFNTENRNKRSLAVDLKNRNSAALIERLIKQTDIVLEGFRPGVAKRLGI
                     DFLSLSKFNSSLIYCSISGYGQTGPWRERPGHDVNYVAAAGALCFPGQWLKPPSRSSL
                     AIADMAGG"
     gene            complement(508..1203)
                     /locus_tag="VGO02_09510"
     CDS             complement(508..1203)
                     /locus_tag="VGO02_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7477958.1"
                     /translation="MLLEGDAALVTGAGNGIGRGIADALEQEGAKVLRADLAEADLSR
                     PEGAVQLAEEAIRKLGKVSIYVHCASPRRQESQTALNVSYEQWREMLAVNLDAAFLIS
                     QKISRHMIAQGVKGRILLVSSLHAESPRNLPHYSAAKAGQVMLVKELARALGPHGIRV
                     NAIAPGAIPGGGFQAPPSMLERIALRRFGTPADVASMAVAVLSERFGAYVTGSTVVVD
                     GGMALHNWIDPPR"
     gene            complement(1193..1561)
                     /locus_tag="VGO02_09515"
     CDS             complement(1193..1561)
                     /locus_tag="VGO02_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV7477959.1"
                     /translation="MRYLVRPADVTPYSPANHTGTRNFRLIGPETVGASQVEVLIGEI
                     ERGKGALPHAHPGIEQVCYLLEGAAHVEVAGEKFEMRPGEACFFPADAMHVFTVTSER
                     AKVMVIYAPPYLEKNARLAP"
     gene            1645..2220
                     /locus_tag="VGO02_09520"
     CDS             1645..2220
                     /locus_tag="VGO02_09520"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7477960.1"
                     /translation="MTVAHRERVLRAATSSFLAHGYRSSVDEIARRAGVAKQTVYHHF
                     PNKDELFKEVARDLAQRVLIELESPPADLREGLLRFALAYRQRTLGAQAISAFRTLVP
                     EVPRFRAVARAMYGSSAGEMVRRLAEYLERAMQSGHLRRDDAHFAAEMLLSMLAGLDR
                     MKRLFSVADEGESEAHRTARIVDCFLRAYQK"
BASE COUNT          382 a          779 c          766 g          363 t
ORIGIN      
        1 ctgccgcccg ccatgtcggc gatggcgagg ctggaacgcg agggcggctt cagccactgg
       61 ccgggaaagc acagcgcgcc cgccgcggcg acgtagttga cgtcgtggcc cggccgctcg
      121 cgccacggcc cggtctggcc gtagcccgaa atggagcagt agatcaaaga ggaattgaat
      181 tttgacaaag acagaaaatc tattcccagg cgcttcgcga cgcccggacg gaaaccctcg
      241 agcacgatgt cggtctgctt gattagtctt tcaatcagcg ctgctgaatt cctgttcttc
      301 aaatcgacgg caaggctgcg cttgttgcgg ttctcggtat tgaactgcac cggcacgtag
      361 gagcggcccg gatcgccctt cggctgctcg accttgatca cctcggcgcc cagatcggcg
      421 agcagcgccg agcagaacgg ccccggcacg aacatcgaga agtcgacgac gcgcaggccg
      481 gcaaggggaa gccagggctc ggccatgtca gcgcggcggg tcgatccagt tgtgcagcgc
      541 catgccgccg tcgaccacca cggtcgagcc ggtgacgtag gcaccgaagc gctcggagag
      601 caccgccacc gccatcgacg ctacgtcggc cggggtgccg aagcggcgca gcgcaatcct
      661 ctcgagcatg ctgggcgggg cctgaaagcc gccacccggg atcgccccgg gcgcgatggc
      721 gttcacgcgg atgccgtgcg ggccgagcgc gcgcgccagc tccttcacca gcatcacctg
      781 ccccgccttg gccgcgctgt agtgcggcag gttgcgcggg ctctcggcgt gcagcgaaga
      841 gacgagcagg atccttccct tcacgccctg cgcgatcata tgtctggaaa ttttttgaga
      901 aatcaagaaa gcggcatcga ggttgaccgc gagcatttcg cgccactgct cgtaactgac
      961 attcagcgca gtctggcttt cctgccggcg cgggctggcg caatgaacgt agatcgagac
     1021 cttgccgagc ttgcggatcg cctcctcggc gagctgcacc gcgccctccg ggcgcgacag
     1081 gtcggcctcg gcgaggtcgg cgcggagtac tttcgccccc tcctgctcca gcgcatccgc
     1141 gatgcctcgg ccgatgccat tgccggcgcc ggtgaccagc gcggcatccc cctcaaggag
     1201 caagccgggc attcttctcg aggtaaggcg gcgcgtagat gaccatcacc ttggcgcgtt
     1261 cgctggtgac ggtgaagacg tgcatggcgt cggccgggaa gaagcaggcc tcgcccggac
     1321 gcatctcgaa tttctcgccg gccacttcga cgtgcgccgc gccctcgagc aggtagcaga
     1381 cctgctcgat gccggggtgg gcatggggca gcgccccctt gccgcgctcg atctcgccga
     1441 tcagcacctc gacctggctc gcccccaccg tttccgggcc gatcagccgg aagttgcgcg
     1501 tgccagtgtg gttggcgggg gagtaaggcg ttacgtcggc aggccggacg aggtagcgca
     1561 tcgggccagt atcccacgct tctggtagac tggtcagtct agtcaggatc gctgcttgcc
     1621 gaggcttccc ggggacaaaa gagcatgacc gtcgcacacc gcgagcgcgt tctgcgcgcc
     1681 gccaccagct ccttcctcgc ccacggctac cgctcgagcg tggacgagat cgcgcggcgc
     1741 gccggggtcg ccaagcagac cgtctaccac cacttcccga acaaggacga gctgttcaag
     1801 gaggtggcgc gcgacctggc gcagcgcgtg ctgatcgagc tcgagtcgcc gccggccgac
     1861 ctgcgcgagg gcctgctgcg cttcgccctc gcctaccgcc agcgcacgct cggcgcgcag
     1921 gccatctccg ccttccgcac gctggtgccc gaggtgccgc gcttccgcgc ggtggcgcgg
     1981 gcgatgtacg gcagcagcgc cggcgagatg gtgcgccgcc tcgccgagta cctggagcgc
     2041 gcgatgcagt ccggccacct gcgccgcgac gacgcgcact tcgccgccga gatgctgctc
     2101 agcatgctcg ccgggctgga ccgcatgaag cggctgttca gcgtcgccga cgaaggggaa
     2161 agcgaggcgc accgcacggc gcgcatcgtc gactgttttc tacgggcgta ccagaaatga
     2221 aaaatctact gatcgtcatc gcggcgctgg tcgtcaccgg ctgtacccag ggcgacgcga
     2281 agcagaagga
//
LOCUS       DASYJA010000436         3999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_338230, whole genome shotgun sequence.
ACCESSION   DASYJA010000436 DASYJA010000000
VERSION     DASYJA010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338230"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /gene="tolB"
                     /locus_tag="VGO02_09525"
     CDS             <1..1262
                     /gene="tolB"
                     /locus_tag="VGO02_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEV7477961.1"
                     /translation="VLAFLLLLAAPIARAQLSIEITGGGAQRIPIAVVAFAGEAALGA
                     SITAIVRADLERSGLFRALEVPPLVPHPTETAKVNYPEWRSRLADALVLGSVAARPDG
                     RFEVRFKLFDTAKQQDLSGIAYTLSKDQARATAHRIADFVYEKLIGEKGTFSTRIAYV
                     VKRGNRYELQIADADGLGEETALASFEPIISPVWSPDGKRLAYVSFEAKRPVVYVHSL
                     LDGKRQVAANFRGSNSAPAWSPDGTRLAVCLSREGGSQIFLINPDGANLRRLTSSSAI
                     DTEPRFSPDGQWIYFTSDRGGSPQIYRIAAGGGEPQRVTFEGSYNVTPRLSPDGKMLA
                     YITRNDGKFQVALLDLANRQTQIITDSDRDESPSFSPNGRMILLATVTGGRGVLSAVS
                     ADGRFKQRLSAAAGGDLREPAWGPFIE"
     gene            1280..1822
                     /gene="pal"
                     /locus_tag="VGO02_09530"
     CDS             1280..1822
                     /gene="pal"
                     /locus_tag="VGO02_09530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEV7477962.1"
                     /translation="MRQIAIAAFSTTVLFLAACATTSDTSAPAPVDTGKPPAQVQTQP
                     VKPVDVTGKPGATALSALKDPKSILSQRSIFYDYDKYDVRDEYRALVEAHAKYLRENP
                     SARMLIQGNTDERGSREYNVGLGQRRSDGVKRMLTLLGARDNQVESVSLGEEKPQDPA
                     HSEAAWSKNRRSDILYSGEY"
     gene            1822..2616
                     /gene="ybgF"
                     /locus_tag="VGO02_09535"
     CDS             1822..2616
                     /gene="ybgF"
                     /locus_tag="VGO02_09535"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEV7477963.1"
                     /translation="MRKNLANALAAAALASAAFPCVAQGLFDDNEARRRIELLRQSVE
                     TSQRTMEERLTKIEGAASDRGAMLELSSQLEALRTEMARMRGQIEVLTNQTEMADKRQ
                     KDLYLDIDTRLRKLEQSREQQAAASDKPAAAEAEASPAETKAYTAALDQFKLGNYALA
                     VSAMQGFMVTYPNSSLAPNAQYWIGMAHSGQRDYKSAIAAQRKLLAAWPDNPKAPDAM
                     LSIASAQETMGAKGEAQKTLQELIARYPGSSASVSARQRLAASGKR"
     gene            2691..3731
                     /locus_tag="VGO02_09540"
     CDS             2691..3731
                     /locus_tag="VGO02_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEV7477964.1"
                     /translation="MGNKTRFCTMGAVSDWVNMGDLGRMRMWLLAIAVALLGSSALHL
                     AGVIDLGKSIYPGPNFTWLSYIVGGFLFGVGMTLGSGCGSKTLIRIGHGSLKSLVVYV
                     FLGIAAYMTLRGILGAFRVEVLEKATLALAPGQDLPSLFASSTGVAKGTWIVIIALLI
                     GGAMAAFVYADREFRGTFDYTLGGVVTGLVVVGGWYVSGHVGYLAEDPNTLQEAFVRT
                     NTGRMESFSFVSPLAYTLEYLMFWTDKSKVITYGIASAAGVIAGSAAYAIATRTFRWE
                     GFRDAEDTATHIIGGLLMGFGGITALGCTIGQAITGFSTLALGSIMTFIAIVAGAAAT
                     MKLQYWRMTREG"
     gene            3780..3855
                     /locus_tag="VGO02_09545"
     tRNA            3780..3855
                     /locus_tag="VGO02_09545"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3813..3815,aa:Lys,seq:ttt)
BASE COUNT          679 a         1410 c         1280 g          630 t
ORIGIN      
        1 ccgtcctagc cttcttgctc ctcctcgctg cgcccatcgc ccgggcgcag ctttccatcg
       61 agatcacggg cggcggcgcg caacgcatcc cgatcgcggt ggtggccttc gccggcgagg
      121 cggcgctcgg cgcgagtatt accgcgatcg tgcgcgccga cctggagagg agcgggctgt
      181 tccgcgccct cgaggtgccg ccgctcgtgc cgcatccgac cgagacggcc aaggtcaact
      241 acccggagtg gcgctcgcgg ctcgccgacg cgctggtgct cggctcggtc gcggcgcggc
      301 ccgacggccg cttcgaggtg cgcttcaagc tgttcgacac cgccaagcag caggacctga
      361 gcggcatcgc ctataccctc tccaaggacc aggcgcgcgc cacggcgcac cgcatcgccg
      421 acttcgtcta cgagaagctg atcggcgaaa agggcacctt ctccacccgc atcgcctacg
      481 tggtgaagcg cggcaaccgc tacgagctgc agatcgcgga tgccgacggc ctgggcgagg
      541 agaccgcgct ggcttcgttc gagccgatca tctcgccggt ctggtcgccc gacggcaagc
      601 gcctcgccta cgtgtcgttc gaggccaagc gcccggtggt ctacgtgcat tcgctgctcg
      661 acggcaagcg ccaggtggcg gcgaacttcc gcggctcgaa ctcggcgccg gcctggtcgc
      721 ccgacggcac gcggctggcg gtgtgcctgt cgcgcgaggg cggctcacag atcttcctga
      781 tcaaccccga cggagccaac ctgcgccggc tgaccagctc gtcggcgatc gacaccgagc
      841 cgcgcttctc ccccgacggc cagtggatct acttcacctc ggaccgcggc ggcagcccgc
      901 agatctaccg cattgcggcc ggcggcggcg agccgcagcg ggtgaccttc gagggtagct
      961 ataatgtgac cccgcgcttg agccccgacg gcaagatgct cgcctacatc acgcgcaacg
     1021 acggcaaatt ccaggtggcg ctgctcgacc tcgccaaccg ccagacgcag atcatcaccg
     1081 acagcgaccg tgacgaatct cctagctttt cgcccaacgg ccgcatgatc ctgttggcca
     1141 ccgtgaccgg cgggcgcggt gtgctttcgg cggtctccgc cgacggccgc ttcaagcagc
     1201 gcctgagcgc cgccgccggc ggcgacctgc gcgagcccgc ctggggcccg ttcatcgaat
     1261 gaagaaaagg agtgatgcca tgcgccagat agcaatcgca gccttctcga caaccgtcct
     1321 gttcctggcc gcctgcgcca cgacgtcgga cacctcggcg ccggccccgg tcgacaccgg
     1381 caagccgccg gcgcaggtgc agacccagcc ggtcaagccg gtcgacgtca ccggcaagcc
     1441 cggcgcgacg gcgctcagcg cgctcaagga cccgaagagc atcctctcgc agcgctcgat
     1501 cttctacgac tacgacaagt acgacgtgcg ggacgagtac cgcgcactgg tcgaggcgca
     1561 cgccaaatac ctgcgggaga acccgagcgc gcgcatgctg atccagggca acaccgacga
     1621 gcgcggcagc cgcgagtaca acgtcggcct cggccagcgc cgttccgacg gcgtcaagcg
     1681 catgctgacg ctgctcggtg cgcgcgacaa ccaggtcgag tcggtgagtc tgggcgagga
     1741 gaagccgcag gaccccgccc acagcgaggc ggcgtggtcg aagaaccgcc gcagcgacat
     1801 cctgtatagc ggcgagtatt gatgcgcaag aatctggcaa atgcgctcgc cgccgccgcg
     1861 ctcgcctcgg cagcgttccc ttgcgtagcg cagggcctgt tcgacgacaa cgaggcgcgc
     1921 cgccgcatcg agctgctgcg ccagagcgtc gagaccagcc agcgcacgat ggaggagcgg
     1981 ctgaccaaga tcgagggcgc cgcatccgac cgcggcgcga tgctcgagct gtcgagccag
     2041 ctcgaggcgc tacgcaccga gatggcgcgc atgcgcgggc agatcgaggt gctcaccaac
     2101 cagaccgaga tggccgacaa gcggcagaag gacctctacc tcgacatcga cacgcggctg
     2161 cgcaagctcg agcagtcgcg cgagcagcag gccgccgcca gcgacaagcc cgccgcggcg
     2221 gaggccgagg cctcgccggc ggaaaccaag gcctacacgg cggccctcga ccagttcaag
     2281 ctcggcaact acgcgcttgc ggtgtcggcg atgcagggct tcatggtcac ctaccccaac
     2341 agcagcctgg cgccgaacgc ccagtactgg atcggcatgg cgcactccgg ccagcgcgac
     2401 tacaagagcg cgatcgcggc gcagaggaag ctgctcgccg cctggcccga caatccgaag
     2461 gcgcccgatg cgatgctcag catcgcgagc gcgcaggaaa cgatgggcgc gaagggcgag
     2521 gcgcagaaga ccctgcagga gctgatcgcg cgctaccccg gtagcagcgc ctcggtcagt
     2581 gcgcgccagc gcctggctgc ctccggcaaa cgttgaacga agccagtccg gcacagcttg
     2641 ctccgtacgt aatcggcggg gcgtttctgc tggccttcgt cttcggcgcc gtgggcaaca
     2701 agactcgctt ctgcaccatg ggcgcggtgt ccgactgggt caacatgggc gacctcggcc
     2761 gcatgcgcat gtggctcctc gccatcgcgg tggcgctgct cggctcgagc gcgctgcacc
     2821 tcgccggcgt catcgacctg ggcaaatcca tctacccggg accgaatttc acctggctgt
     2881 cgtacatcgt cggcgggttc ctcttcggcg tcggcatgac cctcggctcg ggctgcggct
     2941 cgaaaacgct gatccgcatc ggccacggca gcctgaagtc gctggtggtc tacgtattcc
     3001 tcggtatcgc cgcttacatg acgctgcgcg gcatcctggg cgccttccgc gtcgaggtcc
     3061 tcgagaaggc cacgctcgct ctcgcgccgg ggcaggatct gccttcgctc tttgcttcct
     3121 ccaccggggt cgccaagggc acctggatcg tcatcattgc tctcctgatc ggcggcgcga
     3181 tggctgcctt cgtctacgcg gaccgggaat tccgcggcac cttcgactac accctggggg
     3241 gcgtggtcac cggcctggtg gtcgtcgggg gctggtacgt gagcgggcat gtcggctacc
     3301 tcgccgagga cccgaacacg ctgcaggagg ccttcgtgcg caccaacacc ggccgcatgg
     3361 agtccttctc cttcgtttcc ccgcttgcct acacgctcga atacctgatg ttctggaccg
     3421 acaagtccaa agtcatcacc tacggcatcg cctccgcggc cggcgtcatc gccggctcgg
     3481 cggcctacgc catcgccacc cgcaccttcc gctgggaggg attccgcgac gccgaagaca
     3541 ccgcgacgca catcatcggc ggcctgctga tgggcttcgg cggcatcacc gccctgggct
     3601 gcaccatcgg ccaggcgatc accggcttct ccacgctcgc gctcggctcg atcatgacgt
     3661 ttatcgccat cgtcgccggc gctgcagcaa ccatgaagct ccaatactgg cgaatgaccc
     3721 gcgagggctg ataaaattcc gccctttcct gcagttagca ggtctgcgac agaaactacg
     3781 ggtcgttagc tcagctggta gagcagcgga cttttaatcc gttggtcggc ggttcgaatc
     3841 cgccacggcc taccaacaat taaggctcta gcctcggcta gggccttttt tcttggactt
     3901 tccgtcgcaa gtagagactc gaaaggaagc gagcatgcct aaagcgtact gggtgatctg
     3961 ccatcgttcg accaagagcc tggagaaatt cgcagcatt
//
LOCUS       DASYJA010000437         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_793092, whole genome shotgun sequence.
ACCESSION   DASYJA010000437 DASYJA010000000
VERSION     DASYJA010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793092"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VGO02_09550"
     CDS             <1..834
                     /locus_tag="VGO02_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HEV7477965.1"
                     /translation="LLVPLAAKRLQACGAGALAGPMISTWPNERRRQLAVSRLCGLLA
                     SAVADSMRKARLPCVIGGDHSCAGGTWTGVARALHEKGHGSLGLVWIDAHMDAHTPAT
                     SHTGRLHGMPLAWLLGQDDDPLYGLASGVIEPQHVCLVGVRSFEREEQQRLEQLGVRV
                     IHMDEVRGRGVDAALQEAVGIASRGTAAYGISIDLDVVAPEEAPGVGTPVAGGITGSD
                     LARALARIGGGARLAAVELVEYLPRLDRDGCSAAVAVELIAAALCGPRQDAQVLADAI
                     E"
     gene            complement(784..1845)
                     /gene="ribD"
                     /locus_tag="VGO02_09555"
     CDS             complement(784..1845)
                     /gene="ribD"
                     /locus_tag="VGO02_09555"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006496600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEV7477966.1"
                     /translation="MFSAFDHAMMRRALDLAERGLFTTTPNPRVGCILTQRDEVVGAG
                     WHEKAGGPHAEALALAQAGARSAGATVYVTLEPCNHQGRTPPCVEALKRAKVARVVAA
                     MRDPNPQAARGGKALATAGVRFESGLMEEEAKELNIGFVSRMTRGRPWVRLKIAATLD
                     GRTALEDGKSQWITGPEARRDGHRWRARACAVLTGAGTVLADDPRLTVREVETPRQPL
                     RVVVDSRLETPPGARILKGDSVLVFSGRAGKLENAEVVALPNQERKVDLAGMLEELAR
                     REINELHVEAGSRLNASLMREGCVDEFLLYLNPSFLGDSAQGMFDFPTLASLDERIKL
                     GIVSLDRVGEDLRILARPA"
     gene            complement(1865..2332)
                     /gene="nrdR"
                     /locus_tag="VGO02_09560"
     CDS             complement(1865..2332)
                     /gene="nrdR"
                     /locus_tag="VGO02_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEV7477967.1"
                     /translation="MKCPFCGSEDTQVVDTRANLEANTIRRRRKCVQCEKRFTTYERV
                     DLKMPRLVKKDGSRTDFDREKLMGSMMLALRKRPVATEEVEGAVDRVVERLRSLGERE
                     VSTSRVGDFVIRELAKLDKVAYIRFASVYRSFETPEDFRDALLEVKAAKKRRK"
BASE COUNT          320 a          838 c          829 g          358 t
ORIGIN      
        1 ctactcgtac cgcttgccgc caagcgcctg caggcgtgcg gcgccggggc gctcgccggg
       61 ccgatgattt cgacctggcc gaacgagcgg cgcaggcagc tcgcggtgag ccgcctgtgc
      121 ggcctgctcg cctcggccgt cgccgattcg atgcgcaagg cgcgcctgcc atgcgtgatc
      181 ggcggcgacc acagttgcgc cggcggcacc tggaccggcg tggcgcgggc gctgcacgag
      241 aaaggccacg gctcgctcgg cctggtgtgg atcgacgccc acatggatgc gcatacgccg
      301 gccaccagcc acaccggacg cctgcatggc atgccgctcg cctggctgct cggccaggat
      361 gacgacccgc tttatggcct cgcctccggc gtgatcgagc cgcagcacgt ctgcctggtg
      421 ggggtgcgca gcttcgagcg ggaggaacag cagcgtctgg agcagctcgg ggtgcgcgtc
      481 attcacatgg acgaggtgcg cgggcgcggc gtcgacgcgg cgctgcaaga agcggtcggc
      541 atcgccagcc ggggcaccgc ggcctacggc atcagcatcg acctcgacgt ggtcgcgccc
      601 gaagaagcgc ccggcgtggg cacgccggtc gccggcggca tcacaggcag cgacctggcg
      661 cgcgcgctcg cgcgcatcgg cggcggcgcg cgcctggccg ccgtcgagct ggtcgagtac
      721 ctgccgcgcc tggatcgcga cggctgcagc gcggccgtgg cggtcgagct gatcgccgcg
      781 gcgctatgcg ggccgcgcca ggatgcgcag gtcctcgccg acgcgatcga gtgacacgat
      841 gcccagcttg atccgctcgt cgagcgaggc gagcgtgggg aaatcgaaca tgccctgcgc
      901 gctgtcgccg agaaagctcg ggttgaggta gagcaggaac tcgtcgacgc agccctcgcg
      961 catcagcgac gcgttgaggc gcgacccggc ctcgacgtgc agctcgttga tctcgcgccg
     1021 ggcgagctcc tccagcatcc cggccagatc gaccttgcgc tcctggttcg gcagcgccac
     1081 gacctcggcg ttttccagtt ttcccgcccg ccccgaaaaa accaggacgc tgtcgccctt
     1141 caggatcctc gcccccggcg gcgtctccag gcggctgtcg accactacgc gcaggggctg
     1201 gcgcggcgtc tcgacctccc gcacggtgag ccgcggatcg tcggcgagca ccgtgccggc
     1261 gccggtgagc acggcacagg cgcgcgcgcg ccagcggtgg ccatcgcgcc gcgcttcggg
     1321 gccggtgatc cactgcgact tgccgtcctc gagcgccgtg cggccgtcga gcgtcgccgc
     1381 gatcttgagc cgcacccacg gccggccgcg cgtcatgcgc gagacgaagc cgatgttgag
     1441 ctccttcgcc tcctcctcca tcaggccgga ttcgaagcgc acgccggcgg tggcgagcgc
     1501 cttgccgccg cgcgccgcct gcgggttcgg atcgcgcatg gccgcgacga cgcgcgccac
     1561 cttcgcccgc ttcagcgcct cgacgcaggg cggcgtgcgg ccctgatggt tgcagggctc
     1621 gagcgtcacg tacacggtcg caccggcgga gcgggcgccg gcctgggcaa gggcgagagc
     1681 ctcggcgtgc ggtccaccgg ctttttcgtg ccagcccgca ccgacgactt cgtcgcgctg
     1741 cgtaaggatg catcccaccc ggggattggg cgtcgtcgtg aacaacccgc gctccgcgag
     1801 atcgagcgca cgccgcatca tggcgtggtc gaaggcggag aacaggctca ctttgcgctg
     1861 cggatcattt acgtcgtttc ttcgccgcct tcacttcgag cagcgcgtcg cggaaatctt
     1921 ccggcgtctc gaagctccgg tagaccgagg cgaagcggat gtaggccacc ttgtcgagct
     1981 tggcgagctc gcggatgacg aaatcgccga cccggctggt cgacacctcg cgctcgccga
     2041 gggagcgcag gcgctcgacc acgcggtcga ctgcgccctc gacctcctcg gtggccaccg
     2101 ggcgcttgcg cagcgcgagc atcatgctgc ccatcagctt ctcgcggtcg aagtcggtgc
     2161 ggctgccgtc cttcttcacc aggcgcggca tcttcaggtc gacccgctcg taggtggtga
     2221 agcgcttctc gcactgcacg cacttcctgc ggcggcgaat cgtgttcgcc tcgaggttcg
     2281 cccgggtgtc gaccacctgg gtgtcctccg agccgcagaa cgggcatttc atgggtagac
     2341 gggga
//
LOCUS       DASYJA010000438         3025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_975173, whole genome shotgun sequence.
ACCESSION   DASYJA010000438 DASYJA010000000
VERSION     DASYJA010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975173"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VGO02_09565"
     CDS             <1..296
                     /locus_tag="VGO02_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HEV7477968.1"
                     /translation="YKMKGNIHWVSARHAYAAPVHLYDRLFSVANPGADRDFVEDLNQ
                     DSKRTITAQLEPALRYAKVGESFQFERHGYFVKDPKDGGFNRTVILRDSWTKG"
     gene            293..775
                     /locus_tag="VGO02_09570"
     CDS             293..775
                     /locus_tag="VGO02_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEV7477969.1"
                     /translation="MTSLGAAVGALLKERGQTLAVAESSAGGLINAALVAVPGASAYY
                     LGGVIVYTRPGRSALLGITKEDMAGIRSASEPFAKLLAARAQRNLGATWGLAETGAAG
                     PTGNRYGDPAGHACLAVAGPVEAAITFKTWSADREANMWAFTRRALELLEECLRKAAK
                     "
     gene            810..2099
                     /locus_tag="VGO02_09575"
     CDS             810..2099
                     /locus_tag="VGO02_09575"
                     /inference="COORDINATES: protein motif:HMM:NF013254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477970.1"
                     /translation="MPAKFLFVSYTGLIADIAWQIVREGHEARLYIEDPEEKEIGDGF
                     VPKTTDWRKEVDWADVVVFDDVLGMGAMAEELRGKGKLVVGGSAYTDRLEDDRGFGQQ
                     ELKNAGVSIIPQENFTSFDDAVSYVTANPNRYVIKPSGEAQNNKRLLFVGEEDDGKDV
                     IQVLGDYKRAWSDKIKEFQLQRRVVGIEVAVGAFFNGKEFVLPINVNFEHKKLFPGDI
                     GPPTGEMGTSMFWSEPNRIFNATLKKMEAKLAEEKYTGYIDVNCIVNNNGIYPLEFTA
                     RFGYPCISIQHEGLLTPLGELLRGLADGSLSRVRAKSGFQIGVRIVVPPFPYRDMETL
                     ESTSKDAVILFKTPSREGYHIEDVRTAEGEWLVTGTSGVVLIACGTGPTMKQAQRMAY
                     NRVKNVMIPHMYYREDIGDRWFEEDSDRLHAWGYLRD"
     gene            complement(2118..2465)
                     /locus_tag="VGO02_09580"
     CDS             complement(2118..2465)
                     /locus_tag="VGO02_09580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7477971.1"
                     /translation="MVDDNADMRLAAKLLLESEGYVVELAKNGGDALRIQRERPVQIL
                     VTDLFMPDVDGLETVQRFRAEYPDMPIIVISGGGSKLPAKADHLAVARELGVHTLRKP
                     FPPQALLDALLTV"
     gene            2606..>3025
                     /locus_tag="VGO02_09585"
     CDS             2606..>3025
                     /locus_tag="VGO02_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477972.1"
                     /translation="MTKLAAVIALSVFAATASAQGWRASSDQTVGGFKFPESAAYDAK
                     GKVLYVGNFGGDKLAPAEKDGQGYISKVDLNGKMIEERWVPAKGGEPLNKPKGIWIQG
                     ERLWVTDIDVVWIFDLKTKKGRKLALPGVSFANDPAVM"
BASE COUNT          564 a         1009 c          995 g          457 t
ORIGIN      
        1 agtacaagat gaaaggcaac atccactggg tgagcgcgcg gcacgcctat gccgcgccgg
       61 tgcacctcta cgaccgcctg ttcagcgtcg ccaaccccgg cgccgaccgc gatttcgtcg
      121 aggacctgaa ccaggactcc aagcgcacca tcaccgccca gctcgagccg gcgctgcgct
      181 atgcgaaggt gggcgagagc ttccagttcg agcgccacgg ctacttcgtc aaggacccga
      241 aggacggcgg cttcaaccgg accgtcatcc tgagggattc ctggaccaaa ggctgacctc
      301 gctcggcgcg gcagtcggcg cgctcctcaa ggagcgcggg cagacgctcg ccgtggccga
      361 atcctcggcc ggcggcctga tcaacgcggc gctggtcgcc gtgccgggcg cctccgcgta
      421 ctacctcggc ggcgtcatcg tctatacccg gcccgggcgc tcggccctgc tcggcatcac
      481 gaaagaggac atggccggga tccgctcggc gagcgagcct ttcgcgaagc tcctcgccgc
      541 ccgcgcgcag cgcaacctgg gcgcgacgtg gggcctcgcc gagaccggcg ccgccggccc
      601 gaccggcaac cgctacggcg atccggccgg ccacgcctgc ctggccgtcg ccggtccggt
      661 cgaggcggcg atcaccttca agacctggag cgccgaccgc gaagccaaca tgtgggcctt
      721 cacgcgccgc gcgctcgagc tgctcgaaga atgcctgcgc aaggccgcga aataattccg
      781 tcacgcgggc ttaacatact cgcgcctgca tgcccgccaa attcctcttc gtctcgtata
      841 ccggcctgat cgccgatatc gcctggcaga tcgtcaggga gggccacgag gcgcgcttgt
      901 acatcgagga cccggaggaa aaggagatcg gcgacggctt cgtgccgaaa accaccgact
      961 ggaggaagga agtggactgg gccgacgtcg tggtgttcga cgacgtgctg ggcatgggcg
     1021 cgatggccga ggaactgcgc gggaagggca agctggtggt cggcggctcg gcctacaccg
     1081 accgcctgga ggacgaccgc ggcttcggcc agcaggagct gaagaatgcc ggcgtctcga
     1141 tcatcccgca ggagaacttc acctccttcg acgacgccgt cagctacgtc acggccaacc
     1201 ccaaccgcta cgtcatcaag cccagcggcg aggcgcaaaa caacaagcgc ctgctgttcg
     1261 tcggcgagga ggacgacggc aaggacgtga tccaggtgct cggcgactac aagcgcgcct
     1321 ggtcggacaa gatcaaggag ttccagctgc agcggcgggt ggtcggtatc gaagtggcgg
     1381 tcggcgcgtt cttcaacggc aaggagttcg tgctgccgat caacgtcaac ttcgagcaca
     1441 agaagctgtt ccccggcgac atcggcccgc ccaccggcga gatgggcacc tcgatgttct
     1501 ggagcgagcc caaccggatt ttcaacgcca cgctgaagaa gatggaggcg aagctggcgg
     1561 aggagaaata caccggctac atcgacgtca actgcatcgt caacaacaac ggcatctatc
     1621 ccctggaatt caccgcgcgc ttcggctatc cctgcatcag catccagcac gagggcctgc
     1681 tcaccccgct gggcgagctc ttgcgcggcc tggccgacgg ctcgctatcg cgcgtgcgcg
     1741 cgaagagcgg cttccagatc ggcgtgcgca tcgtggtgcc gcccttcccc tatcgcgaca
     1801 tggagaccct cgagtcgacc tcgaaggacg cggtgatcct gttcaagacg ccgagccgcg
     1861 agggctacca catcgaggac gtgcgcaccg ccgagggcga gtggctggtg acgggcacct
     1921 ccggcgtggt gctgatcgcc tgcggcaccg ggccgaccat gaagcaggcg cagcgcatgg
     1981 cctacaaccg ggtgaagaac gtgatgatcc cgcacatgta ttaccgcgag gacatcggcg
     2041 accgctggtt tgaggaggac agcgaccggc tgcacgcctg gggctacctg cgggattgaa
     2101 agccggactg aagcctttca gacggtgagc agcgcgtcga gcagcgcctg cggcgggaag
     2161 ggcttgcgca gggtgtgcac gcccagctcg cgcgccacgg ccaggtggtc ggccttcgcc
     2221 ggcagcttgc tgccgccgcc cgagatcacg atgatcggca tgtcgggata ctcggcgcgg
     2281 aagcgctgca ccgtctcgag gccgtcgacg tcgggcatga acagatcggt gaccagaatc
     2341 tgcaccggac gctcgcgctg gatgcgcagg gcgtcgccgc cgttcttcgc caactcgacc
     2401 acgtagcctt cgctctcgag cagcagcttc gccgcgaggc gcatgtccgc gttgtcgtcg
     2461 accaccaaca cccgtgttct gcgcccgaag cgcgaccgtc ccatcctcta cacccccatg
     2521 gcgcattgtc cggctcgcgg tcgcggcacg acaaccgcac ggccgtaggt cttacaatgg
     2581 ttcacaattc gttgaggagg aaaacatgac aaaactcgcc gcagttatcg ccttatccgt
     2641 gttcgccgct actgcgtccg cgcagggctg gcgggcttcg tccgaccaga cggtgggcgg
     2701 cttcaagttt cccgagtcgg ccgcctacga cgcgaaaggc aaggtgctct acgtcggcaa
     2761 tttcggcggc gacaagctcg cgccggccga gaaggacggc cagggctaca tcagcaaggt
     2821 cgacctgaac ggcaagatga tcgaggagcg atgggtaccg gcgaagggcg gcgagccgct
     2881 caacaagccc aagggcatct ggatccaggg cgagcgcctg tgggtcaccg acatcgacgt
     2941 ggtgtggatc ttcgacctga agaccaagaa gggccgcaag ctcgccctgc ccggcgtgag
     3001 cttcgccaac gatcccgcgg tgatg
//
LOCUS       DASYJA010000439         5284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2971456, whole genome shotgun sequence.
ACCESSION   DASYJA010000439 DASYJA010000000
VERSION     DASYJA010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2971456"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1736
                     /locus_tag="VGO02_09590"
     CDS             <1..1736
                     /locus_tag="VGO02_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEV7477973.1"
                     /translation="FDEFCRIAVARGGFALARIIQIDPAGKASIAATTEADPAVFQAL
                     VDTYNRDPAGADSLLAKALRSGQAVISNDVAADERATNRAELTRGGNYALALLPVVVE
                     ERVAGLVALRAREPGFFDHEELRLLSEMIANISFALELIGKQDRISYLALYDTLTGLP
                     NRTLFHERLTQAIDAAQRAAAPLALVLIDLERFKAINDTLGQDVGDRVLQQVAQRLRD
                     STGDLHRVARLGNDVFALMFTDITTAEEVARRLESSAAKFFAKPFQVDSHEVRLAGKA
                     GIAVYPDDGVDADTLFRNAEASLKRAKETGERYLFYAPHINARVSEQVDLENRLRKAV
                     DEGELFLHYQPKVDMGSRAIVGVEALMRWQQPGEGGLISPARFVPVLEQTGLIFEAGR
                     QAVAAASAMHRRWQAAGLPAPRIAVNVSALQLRRRTFVEDVLGAVGDLGADGGGLDLE
                     ITESLLMSDVDESIRKLRSLRETGMHIALDDFGTGYSSLAYLSKLPVDTLKIDRGFVR
                     GMTEKSEDTSIISIIISLAQALRLKTVAEGVESEQQAQLLRLLRCDQAQGYLFSPPVA
                     AERIEALLKAV"
     gene            1759..2985
                     /gene="hemA"
                     /locus_tag="VGO02_09595"
     CDS             1759..2985
                     /gene="hemA"
                     /locus_tag="VGO02_09595"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEV7477974.1"
                     /translation="MSSLYALGLNHQTAPLAVRERVVFHVERLREALGEITRGLAREA
                     AILSTCNRTELYLAGEEPAAAAQWLARYHSLSPSELAPYLYTLPAEPAARHAFRVASG
                     LDSMVLGEPQILGQMKDAARTAESAGTLGTVLHKLFQRSFAVAKEVRSTTQVGATSVS
                     MAAAAVKLAARIFPSLKDQSVLLIGAGEMIELCATHFAAQGPARLTVANRTIERAERL
                     AHRFNGQAIELKELAAHLHEHDIVVSCTASSLPILGKGLVERALRARRRKPVFMVDLA
                     VPRDIEQEAGELDDVFLYTIDDLGEIVAANLDVRRSAVTQAEAIIETQVGQFMHWMQA
                     RDSVPLIRMLREQAEVARQHEIERALKALQRGEPPAQIIETLSQALTNKLMHAPTQAL
                     NDASLAELVARLYRLK"
     gene            2982..4067
                     /gene="prfA"
                     /locus_tag="VGO02_09600"
     CDS             2982..4067
                     /gene="prfA"
                     /locus_tag="VGO02_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEV7477975.1"
                     /translation="MKPTLRTKLDSLVGRLEELNQALAAENATRDLDQFRRLSREYAE
                     VSAVVSLFKDYLQVERDADEARDMAADSGMKSYADEELKKARAAMERLEAELQKALLP
                     KDPNDERNTFLEIRAGTGGDESGLFAGDLFRMYTRYAERRGWQVEIISESPSELGGYK
                     EVIVRILGQGVFSRLKFESGGHRVQRVPQTEAQGRIHTSACTVAVLPEADAINEVVLN
                     PAELRVDTFRASGAGGQHVNKTDSAIRVTHLPTGIVVECQDSRSQHKNREKALGVLAA
                     RIRDKQLSEQQAKTASTRKTLIGSGDRSQRIRTYNFPQGRVTDHRINLTLYKIERIMD
                     GELDELLDALAAEHLAEQLSQLAEEAA"
     gene            4064..4855
                     /gene="prmC"
                     /locus_tag="VGO02_09605"
     CDS             4064..4855
                     /gene="prmC"
                     /locus_tag="VGO02_09605"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV7477976.1"
                     /translation="MNVADALRASSIDLREARLLLAAASGFSEAAIAASLERALAPEV
                     EGLFFEYTERRRGGEPVAYIVGFKEFYSMQLAVNPAVLIPRPETELLVDLALQLGFSS
                     IVDLGTGSGAIALAIKRQRPEARVVATDSSAAALVVAQRNAVRHGLEVEFRHGRWLEP
                     LAGERFDLIVANPPYVTDGDHHLRQLGFEPQEAIIGGVDGLDGIREIAREAPGHLLPG
                     GRLLLEHGMGMEQAVREVLDRAGLEDIENWPDLARIPRVTGGKVK"
     gene            4852..5184
                     /gene="grxD"
                     /locus_tag="VGO02_09610"
     CDS             4852..5184
                     /gene="grxD"
                     /locus_tag="VGO02_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEV7477977.1"
                     /translation="MSDMDTQQRIHEQVTSHPVVLFMKGTPQMPQCGFSQLAIQILNA
                     CGVPNPHTVNVLADNEIRQGIKQYANWPTIPQLYINGEFVGGSDIVKEMYESGELQKL
                     LAEAPQKA"
BASE COUNT          884 a         1863 c         1787 g          750 t
ORIGIN      
        1 tgttcgacga gttctgccgc atcgccgtgg cgcgcggcgg cttcgcgcta gcgcgcatca
       61 tccagatcga tcccgccggc aaggcgagca tcgccgccac caccgaagcc gatccagccg
      121 tcttccaggc cttggtcgac acctacaatc gcgatccggc cggcgccgac tcgctgctcg
      181 ccaaggcgct gcgcagcggc caggcggtga tctccaatga tgtcgccgcc gacgagcgcg
      241 ccaccaaccg cgcggagctg acgcgcggcg gcaactacgc cctcgccctg ctgccggtgg
      301 tggtcgagga gcgcgtcgcc gggctggtgg cgctgcgcgc gcgggagccg gggttcttcg
      361 accatgagga gctgcgcctg ctttccgaga tgatcgccaa catctcgttc gcgctcgagc
      421 tgatcggcaa gcaggaccgc atcagctacc tcgcgctcta cgacacgctc accgggctgc
      481 ctaaccgcac gctgttccac gagaggctga cgcaggcgat cgacgccgcg cagcgtgccg
      541 ccgcgccgct cgccctcgtg ctgatcgacc tggagcgctt caaggcgatc aacgacaccc
      601 tcggccagga cgtcggcgac cgggtgctgc agcaggtggc gcagcggctg cgcgactcga
      661 ccggcgacct gcaccgcgtg gcgcgcctgg gcaacgacgt cttcgccctc atgttcaccg
      721 acatcacgac ggcggaggag gtggcgcggc gcctcgagag cagcgcggcg aagttcttcg
      781 ccaagccgtt ccaggtcgac agccacgagg tacggctggc cggcaaggcg ggcatcgccg
      841 tgtacccgga cgacggcgtc gacgccgaca cgctgttccg caacgccgag gcttcgctca
      901 agcgggccaa ggagaccggc gagcgctatc tcttctacgc gccgcacatc aacgcgcgcg
      961 tctcggagca ggtcgacctg gagaaccgcc tgcgcaaggc ggtcgacgag ggcgagctgt
     1021 tcctgcacta ccagccgaag gtcgacatgg ggagccgcgc catcgtcggc gtcgaggccc
     1081 tgatgcgctg gcagcagccg ggcgagggcg ggctgatttc gcccgcgcgc ttcgtgccgg
     1141 tgctcgagca gaccgggctc atcttcgagg ccggcaggca ggcggtggcg gcggcctccg
     1201 ccatgcaccg gcgctggcag gcggccgggc tgcccgcgcc gcgcatcgcc gtgaacgtct
     1261 cggcgctgca gctgcgccgg cgcaccttcg tcgaggacgt cctcggcgcg gtgggcgatc
     1321 ttggcgccga cggcggcggc ctcgacctgg agatcaccga gagcctgctg atgagcgacg
     1381 tcgacgagag catccgcaag ctgcgctcgc tgcgcgagac gggcatgcac atcgcgctcg
     1441 acgacttcgg caccggctac tcgtcgctcg cctacctcag caagctgccg gtcgacacgc
     1501 tcaagatcga ccgcggcttc gtgcgcggca tgaccgagaa gtccgaggac accagcatca
     1561 tctcgatcat catttcgctc gcgcaggcgc tgcgcctgaa gaccgtggcc gagggcgtcg
     1621 aatcggagca gcaggcgcag ctgctgcgcc tgctgcgctg cgaccaggcg cagggctacc
     1681 tgttcagccc gccggtggcg gccgaacgca tcgaggcgct gctcaaggcc gtataatctg
     1741 cggtcccagg cgggccgcat gtcgtctctc tacgcactcg gactcaatca ccagaccgcg
     1801 ccgctcgcgg tgcgcgagcg cgtggtgttc cacgtcgagc gcctgcgcga ggcgctcggc
     1861 gagatcacgc gcggcctggc gcgcgaggcg gcgatcctgt ccacctgcaa ccgcaccgag
     1921 ctgtacctcg cgggcgagga gccggccgcg gccgcgcagt ggctcgcgcg ctaccacagc
     1981 ctgtcgccga gcgagctcgc gccatacctg tacacgcttc ccgccgaacc cgccgcgcgg
     2041 catgcgttcc gcgttgcttc gggcctcgac tcgatggtgc tcggcgagcc gcagatcctc
     2101 ggccagatga aggacgccgc gcgcaccgcc gagtccgccg gcacgctcgg cacggtgctg
     2161 cacaagctgt tccagcgcag cttcgccgtc gccaaggaag tgcgctcgac gacgcaggtc
     2221 ggcgccacca gcgtgtcgat ggccgccgcg gcggtcaagc tcgcggcgcg catcttcccg
     2281 agcctcaagg accagagcgt gctgctgatc ggcgccggcg agatgatcga gctgtgcgcc
     2341 acccacttcg ccgcgcaggg accggcccgc cttacggtcg ccaaccgcac catcgagcgc
     2401 gccgagcgcc tcgcgcaccg cttcaacggc caggcgatcg agctgaagga gctcgccgcg
     2461 cacctgcacg agcacgacat cgtggtgtcg tgcaccgcct cttcgctgcc gatcctcggc
     2521 aaagggctgg ttgagcgcgc cctgcgggca cgccggcgca agccggtctt catggtcgat
     2581 ctcgccgtgc cgcgcgacat cgagcaggag gcgggcgagc tggacgacgt gttcctgtac
     2641 accatcgacg acctgggcga gatcgtcgcc gccaacctgg acgtgcgccg ctccgccgtg
     2701 acgcaggccg aggcgatcat cgagacccag gtcggccagt tcatgcactg gatgcaggcg
     2761 cgcgacagcg tgccgctgat ccgcatgctg cgcgagcagg ccgaggtggc gcggcagcac
     2821 gagatcgagc gcgccctgaa ggcgctgcag cgcggcgagc cgccggccca gattattgag
     2881 acgctctcgc aagcactgac caacaagctg atgcacgcgc cgactcaggc cctgaacgac
     2941 gcatcactgg cggagttggt tgcccgcctg taccgcctga agtgaaaccc acgctgcgga
     3001 ccaagctcga ctccctcgtc gggcgcctgg aggaattgaa ccaggcgctc gccgccgaga
     3061 acgccacgcg cgacctggac cagttccgcc gcctgtcgcg cgaatacgcc gaggtgtccg
     3121 cggtcgtttc cctgttcaag gactatctcc aggtagagcg tgatgccgat gaagcccgcg
     3181 acatggcggc cgactccgga atgaagtcct acgccgacga ggagctgaag aaggcgcgcg
     3241 cggcgatgga gcggctggaa gccgagctgc agaaggcgct gctgccgaag gacccgaacg
     3301 acgaacgcaa caccttcctc gagatccgcg ccggcaccgg cggcgacgag tcgggcctct
     3361 tcgccggcga cctgttccgc atgtacacgc gctacgccga gcgccgcggc tggcaggtcg
     3421 agatcatctc cgagagtccc tccgagctcg gcggctacaa ggaggtgatc gtgcgcatcc
     3481 tcgggcaggg ggtgttctcg cgcctcaagt tcgagtccgg cggccaccgc gtgcagcgcg
     3541 tgccgcagac cgaggcgcag gggcgcatcc acacctcggc ctgcacggtg gcggtgcttc
     3601 ccgaggccga cgccatcaac gaggtggtgc tcaatcccgc cgagctgcgc gtcgacacct
     3661 tccgcgcctc cggcgccggc ggccagcacg tcaacaagac cgactcggcg atccgcgtta
     3721 cccacctgcc caccggcatc gtcgtggaat gccaggactc gcgctcgcag cacaagaacc
     3781 gcgagaaggc gctcggcgtg ctggcggcgc gcatccgcga caagcagctc tccgagcagc
     3841 aggcgaagac ggcttcgacg cgcaagacgc tgatcggctc gggcgaccgc tcgcagcgca
     3901 tccgcaccta caacttcccg caggggcggg tcaccgacca ccgcatcaac ctgacgctct
     3961 acaagatcga gcgcatcatg gacggcgagc tcgacgagct gctcgacgcg ctcgccgccg
     4021 agcacctcgc cgagcagctc tcgcagctcg cggaagaggc cgcttgaacg tcgccgacgc
     4081 gctgcgcgcc agcagcatcg atctgcgcga ggcgcggctt ctgctcgccg ctgccagcgg
     4141 cttctctgaa gccgcgatcg ccgcttccct ggaacgcgca ctcgctcccg aggtcgaagg
     4201 gctgttcttc gaatacacgg aaagaagaag gggcggcgag ccggtggcgt acatcgtcgg
     4261 cttcaaggag ttctacagca tgcagctggc ggtcaacccg gcggtgctga tcccgcggcc
     4321 ggagaccgag ctgctggtgg atctggcgct gcagctgggc ttctcctcga tcgtcgacct
     4381 gggcaccggc agcggcgcga tcgcgctcgc gatcaagcgc cagcggcccg aggcgcgcgt
     4441 cgtggcgacc gactcgagcg cagcggcgct ggtcgtggcg cagcgcaacg cggtaaggca
     4501 cggcctggag gtcgagttcc ggcacggtcg ctggctcgag ccgctcgccg gcgagcgctt
     4561 cgacctgatc gtggcaaatc ccccctacgt taccgacggt gaccaccacc tgaggcaatt
     4621 gggcttcgag ccgcaggagg ccatcatcgg gggcgtagat ggcctcgacg gcatcaggga
     4681 gatcgctcgc gaggcgcccg ggcacctcct gccgggcggg cggctgctgc tcgagcacgg
     4741 catggggatg gagcaggcag tgcgcgaggt gctcgaccgg gcggggcttg aagacatcga
     4801 gaactggccc gatctggcga ggatccccag ggtcaccggc ggaaaggtaa aatgagcgac
     4861 atggacaccc aacagcgcat tcacgagcag gtcactagcc acccggtggt gctcttcatg
     4921 aagggcacgc cgcagatgcc gcagtgcggc ttctcgcagc tcgcgatcca gatcctgaac
     4981 gcctgcggcg tgccgaaccc gcacacggtc aacgtgctgg ccgacaacga gatccgccag
     5041 ggcatcaagc agtacgccaa ctggccgacc atcccgcagc tctacatcaa cggcgagttc
     5101 gtcggcggct ccgacatcgt gaaagagatg tacgagtcgg gcgaactgca gaaactcctc
     5161 gccgaggcgc cgcaaaaagc ctgattcaca aaatggggtc agaccccatt tttccgcagc
     5221 aggcctagcg tcgcctccac ctgctcgagg tagccgccac cgaagatgtt gaggtggttg
     5281 agca
//
LOCUS       DASYJA010000440         2684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3063204, whole genome shotgun sequence.
ACCESSION   DASYJA010000440 DASYJA010000000
VERSION     DASYJA010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3063204"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="VGO02_09615"
     CDS             <1..1142
                     /locus_tag="VGO02_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEV7477978.1"
                     /translation="QGGAPRVHGEAQAALPGKMSLKIESVGLRAVAVPMKRPLQTSIA
                     AVTIAPLLLLDLKTDGGIVGRSYLFAPGKQHLPALVKLVEAMAEVLEGDELAPFDIEK
                     KLRARYALLGVHNMVLFAMSGIDMAVWDALGQALGQPLVRLLGAAPRPIPAYNSNGLG
                     LMPLKPLVKQARELVDEGFWAIKLRLGRPDAREDLAALRAVKEEIGPDVHLMADFNQG
                     LSVAEAIKRGRMIDEEGGTYWIEEPVRADDFSGCSEVRKEVHTPIQIGENFMGPEQMA
                     QALAAGACDYVMPDAERIGGVTGFLRAAALAQGAGREMSSHLFPEMSCHVLAATPTCH
                     WLEYVDWASPILKHPLPVRNGHVSAPDRPGLGMEWDEKAVQRFAV"
     gene            1142..>2684
                     /locus_tag="VGO02_09620"
     CDS             1142..>2684
                     /locus_tag="VGO02_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEV7477979.1"
                     /translation="MFTRHFAHWPPGQPRTIELPRCTVYHNLDARAREHPQRVAVDYY
                     GRRLTYAELKRQVDAMAGFLQRRCGVARGERVLLYMQNCPQFIVAYYAILRADAVVVP
                     VNPMNRTEELRHYVGDSDAAVAIVGQDVFRHIEPIQGLKHFLVATYSEYLGEKSDLPL
                     PDFVRAPRQRFSSEVKYWEEALGAGLQPGNHQAQPEDIAVMPYTSGTTGKPKGCIHTH
                     ASVQATTVPYLYWRGVQAHSVVLSALPMFHVTGMQAGMNAPLHLGATLVVLSRWDREC
                     AAMQIERAGVTNWSAITTMMVDFLANPNLGRYDLSSLKQLGGGGAAMPEAVARKLEEV
                     IGLPFVEGYGLSETMAPTHINPPQRPKRQCAGIPFFNTDARVLDLQDSHELEANQVGE
                     IIVSGPQVFQGYWKQPAASAEAFVEHDGKRFFRTGDLGYYDEDGYFFVTDRLKRMINC
                     SGFKVWPAEVEAMLYAHPAIHEACVIGARDEYRGETVKALVVLKIEFLKKIEPKEIID
                     WAREKM"
BASE COUNT          469 a          870 c          908 g          437 t
ORIGIN      
        1 tacaaggagg ggcgccgcgc gttcatggag aagcgcaagc cgcgcttcca gggaaaatga
       61 gcctgaagat agagagcgtt ggcctgcgcg cggtagcggt gccgatgaag cgtccgttgc
      121 agaccagcat cgccgcggtg acgattgcgc cgctgctgct cctcgacttg aagaccgacg
      181 gcggcatcgt cggccgctcg tacctcttcg ctccgggtaa gcagcacctc ccggcgctgg
      241 tcaagctggt cgaggcgatg gcggaggtgc tggagggcga cgagctcgcg ccgttcgaca
      301 tcgagaagaa gctgcgcgcg cgctatgcgc tgctcggcgt gcacaacatg gtgctgtttg
      361 ccatgtcggg catcgatatg gccgtctggg acgcgctcgg ccaggcgctc gggcagccgc
      421 tcgtgcgcct gctcggcgct gcgccgcggc cgattccggc gtacaactcc aacgggctcg
      481 gcttgatgcc cctcaagccg ctggtgaagc aagcgcgcga attggtggac gagggattct
      541 gggcgatcaa gctgcgcctc ggccggcccg acgcgcgcga ggacctcgcg gcgctgcggg
      601 cggtgaagga ggaaatcggc cccgacgtgc acctgatggc ggacttcaac cagggcctga
      661 gcgtcgccga agccatcaag cgcggccgca tgatcgacga agagggcggt acctactgga
      721 tcgaggagcc ggttcgcgcg gatgattttt cgggatgcag tgaggtacgc aaggaagtgc
      781 acaccccgat ccagatcggc gagaacttca tggggccgga gcagatggcg caggcgcttg
      841 ccgcgggcgc ctgcgactac gtcatgcccg acgccgagcg catcggcggc gtcaccggct
      901 tcctgcgcgc ggcggcgctg gcgcagggcg cgggcaggga gatgtcgagc catctcttcc
      961 cggagatgag ctgccacgtg cttgccgcca ccccgacctg ccactggctg gagtatgtgg
     1021 attgggcctc tccgatcctc aagcatccgc tgccagtccg caacggccat gtttccgcgc
     1081 cagatcgccc cggtcttggc atggagtggg acgagaaagc ggtccagcgc ttcgccgtct
     1141 gatgttcacc cggcatttcg cgcactggcc gccggggcag ccgagaacca tcgagctgcc
     1201 gcgctgcacc gtctatcaca acctcgacgc gcgcgcgcgc gagcatccgc agcgcgtcgc
     1261 cgtcgactac tacggccgcc gcctgaccta cgccgagctc aagcgccagg tcgacgcaat
     1321 ggccggcttc ctgcagcgcc gctgcggcgt ggcgcgcggc gagcgcgtgc tgctctacat
     1381 gcagaactgc ccgcagttca tcgtcgcgta ctacgcaatc ctgcgcgccg acgcggtggt
     1441 ggtgccggtg aatccgatga accgcaccga ggagctgcgc cactatgtcg gggattccga
     1501 cgccgcggtg gcgatcgtcg gccaggatgt cttcaggcac atcgaaccga tccagggcct
     1561 gaagcatttc ctggtggcga cctattccga gtatctcggc gagaaatcgg atttgccgct
     1621 acccgacttc gtgcgcgcgc cgcgccagcg gttttcgtcc gaagtgaaat actgggagga
     1681 ggcgctgggc gcgggccttc aacctgggaa ccaccaagcg caacccgaag acatcgcggt
     1741 gatgccctac acgtccggta ccaccggcaa gccgaagggc tgcatccaca cccacgccag
     1801 cgtgcaggcg accaccgtgc cgtacctgta ctggcgcggc gtgcaggcgc acagcgtggt
     1861 gctgtccgcg ctgccgatgt tccacgtcac aggcatgcag gccggcatga acgcgccgct
     1921 gcacctcggc gcgacgctgg tggtgctgtc gcgctgggac cgcgagtgcg ccgcgatgca
     1981 gatcgagcgg gcaggcgtga ccaactggtc ggcgatcacc accatgatgg tcgacttcct
     2041 cgccaacccg aatctcggcc gctacgacct gagctcgctc aagcagctcg gcggcggcgg
     2101 cgcggcgatg cccgaggcgg tggcgcgcaa gctggaggag gtcatcggcc tgcccttcgt
     2161 cgaaggctac ggcctgtcgg agaccatggc gccgacgcac atcaacccgc cgcagcgccc
     2221 caagcgccag tgcgccggca ttccgttctt caatacggat gcccgggttc tcgaccttca
     2281 ggattctcat gaactggaag ccaaccaggt cggcgagatc atcgtgagcg gtccacaagt
     2341 cttccagggc tactggaagc agccggcggc gagcgccgag gccttcgtcg agcacgacgg
     2401 caagcgcttc ttccgcaccg gcgacctggg ctactacgac gaggacggct atttcttcgt
     2461 caccgaccgc ctgaagcgca tgatcaactg ctcgggtttc aaggtttggc cggcggaggt
     2521 cgaggcgatg ctctacgcgc atccggcgat ccatgaggcc tgcgtcatcg gcgcgcgcga
     2581 cgagtaccgc ggcgagaccg tcaaggcgct ggtggtttta aaaattgaat ttttaaaaaa
     2641 aatcgaacct aaggagataa tcgattgggc gcgcgagaag atgg
//
LOCUS       DASYJA010000441         5720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2699384, whole genome shotgun sequence.
ACCESSION   DASYJA010000441 DASYJA010000000
VERSION     DASYJA010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2699384"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="VGO02_09625"
     CDS             <1..830
                     /locus_tag="VGO02_09625"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV7477980.1"
                     /translation="SHNHGQGHETTFAQIVSRVLGIAVESIRLRTSEAEHNFVANPTG
                     GSRTLHGLGSAMHWASQEIAKNGANLAAEHLESAVQDIEFDLGTYRIKGTDRKVRIEE
                     LAKKYPGRLDLDFKDRPKVPATFPNGCHVAEVEIDPETGDCDIVSYVACDDVGNIINH
                     QIVEGQMQGGVTQGAGHIFLEQGVYDAAGQLLTGSFMDYAMPRAGLVGGLTVTDHAVP
                     TATNPLGAKGVGEGGVTGSMPALMNAVLDALRGAGVKEFEMPASPHRVWRALQAAKG"
     gene            978..1382
                     /locus_tag="VGO02_09630"
     CDS             978..1382
                     /locus_tag="VGO02_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477981.1"
                     /translation="MVDIAMGIAMRSVMALLLVAAADAAAQAMPGEAPAAGSMPSLAL
                     GLSSGGVRALNEEFDEQRRALDAFGGRTSVDRFTNGGFEKPRHWTLYGRLGVFGFQDQ
                     LDAGRSDGIQFKLGRTGPKLTGRTYVGIHRTF"
     gene            1401..1589
                     /locus_tag="VGO02_09635"
     CDS             1401..1589
                     /locus_tag="VGO02_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477982.1"
                     /translation="MADAKEEDPVTPRRSSWSAALAQLPGFPDKVPLPGLPPRGAQAV
                     SPLGKDADETDEPGTPEQ"
     gene            1593..2387
                     /locus_tag="VGO02_09640"
     CDS             1593..2387
                     /locus_tag="VGO02_09640"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEV7477983.1"
                     /translation="MNSGRARLVIAALIALFALVGYFTKTTVNPITGETQRVALTPEE
                     EVALGLKAAPEMAAQMGGLSRNEKARALVQRVGTKLASGGFAAKSPYKFSFHVLADPE
                     TVNAFALPGGPVFITEGLLRRLRTEGELAAVLGHEVGHVLARHSSERLAKEQLTQGLI
                     SAVVVGTGDYTSAQIAQMAGSMINMKYGRGDELEADALGIRIAFESGYDPRAMIGVME
                     VLAQASGGSRQPEFASTHPAPENRQARIKEEIGKRFPSGVPDGLTR"
     gene            2402..2587
                     /locus_tag="VGO02_09645"
     CDS             2402..2587
                     /locus_tag="VGO02_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477984.1"
                     /translation="MLGVLGLLGVGAGDPIPVEPELEPDPVLPDELDPLLGVLLEPEP
                     VLLPVLPEPLLLELAPY"
     gene            2618..2890
                     /locus_tag="VGO02_09650"
     CDS             2618..2890
                     /locus_tag="VGO02_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477985.1"
                     /translation="MPTHWLGRALGLLLSLDELLLPELIEPEPLVPAPALPPAAGLVV
                     ELPGLVAELPVLPEVCAHEALAKPSSAAATAALSVFTITMYAPLVG"
     gene            complement(3006..3710)
                     /locus_tag="VGO02_09655"
     CDS             complement(3006..3710)
                     /locus_tag="VGO02_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7477986.1"
                     /translation="MREPQAIVLVPGLYSTPRFYELQLPALWAFGPVTIADHKRDDSM
                     AGIARRILASAPPRFALAGHSMGGYVAFEILRQAPGRVVKLALLDTAARPDTPEQSER
                     RRSQIAIAAGGRMQAIVELQLPVLVHASRRADAALRRQIELMAEETGAEAFIRQQTAI
                     MNRPDSRPTLAAIRCPSLVLVGEQDELTPPDRAREMAEGIAGARLVTVADCGHMCALE
                     QPERVTQELVAWLERP"
     gene            complement(3710..5236)
                     /locus_tag="VGO02_09660"
     CDS             complement(3710..5236)
                     /locus_tag="VGO02_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEV7477987.1"
                     /translation="MRSVIVPLGLLLSGCGGLPSLEGRSASFALEDTAGTRLARAVAP
                     HVAAHPARTGIYALAEPVDAFAARVLLAAAADRSLDVQYYIWHGDQTGWLLFEAIHRA
                     AQRGVRVRLLLDDNGVAGLDPTLAALDAHPNIEVRLYNPFAQRRARALQYATDFTRLN
                     RRMHNKSFTADSQASIVGGRNVGNEYFGAGRETLFEDLDVLAVGPAVGEVSKAFDLYW
                     NSASAYPVASLLSAPGPQAAAQLEADFAATRADPGSAAYVKALRETPLVSELLAGRLA
                     LEWAEARLLRDDPAKTLDTTGRTDVLLLAELLRSTARAEKSFDLISPYFVPGKEGSAG
                     LEALARRGVAVRVLTNSLAATDVGAVHAGYAKRREALLRAGVRLYELKPTAERDAPRG
                     KRASGSSSASLHAKTFSLDRSRSFVGSFNFDQRSALLNTEMGLMIDSPVLGRRLAEVL
                     DEKLPRAAYEVRLAADGGLEWIERTAAGETRYTTEPGAGMLRRAWIRFLSVLPIEWLL
                     "
     gene            5290..>5720
                     /locus_tag="VGO02_09665"
     CDS             5290..>5720
                     /locus_tag="VGO02_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEV7477988.1"
                     /translation="MKLQKIAVVAAAAAAFAGCSTMNDMRAVMPGSMGGWTTLVDGGK
                     GLENFDRIGNSNWRVEDGAIVADRNPDKTPSYLVTRTSYSNFVIKAEFWASDDANSGI
                     FSRCKDPKNVGDETCYEANIYDQRPDPSYGTGAIVKVAKVDP"
BASE COUNT          889 a         1954 c         2070 g          807 t
ORIGIN      
        1 cgtcccacaa ccacggccag ggccacgaga ctacgttcgc gcagatcgtc tcgcgggtgc
       61 tgggcattgc cgtggagagc atccgcctgc gcacctccga ggcggagcac aacttcgtcg
      121 ccaacccgac cggtggctcg cgcacgctgc atggcctggg cagcgcgatg cactgggcct
      181 cgcaggagat cgcgaagaac ggcgcgaacc tcgcggccga gcacctcgag agcgcggtgc
      241 aggacatcga attcgacctg gggacctacc ggatcaaggg aaccgaccgc aaggtgcgga
      301 tcgaggagct ggcgaagaaa tacccgggca ggctcgacct cgacttcaag gaccgcccca
      361 aggtgccggc cactttcccg aacggctgcc acgtcgccga agtggagatc gacccggaaa
      421 cgggcgactg cgacatcgtg tcctacgtcg cctgcgacga cgtcggcaac atcatcaacc
      481 accagatcgt cgaagggcag atgcagggcg gcgtcactca gggcgcgggg cacatctttc
      541 tcgagcaggg cgtctacgac gccgccggcc agctgctgac cggaagcttc atggactacg
      601 ccatgccgcg cgccgggctg gtcggcgggc tcaccgtgac cgaccatgcc gttccaaccg
      661 cgaccaaccc gctcggcgca aagggcgtcg gggagggcgg cgttaccggc tcgatgccgg
      721 cgctgatgaa cgcggtgctc gacgcgctgc gcggcgcggg agtgaaggag ttcgaaatgc
      781 cggccagccc gcaccgcgtg tggcgggcgc tgcaggccgc gaagggatag ttcttccggc
      841 gcagggctgc gactttctgc cgtagctttc acgcgacagg aggctcggca acgctacagc
      901 ggactattgg ttgctcgcac tcgggttcat ctgcgacgcc gatcatggcg tggtagcgga
      961 gagagcgagt gcgcgtagtg gtcgacatag caatgggcat cgccatgcgg agcgtgatgg
     1021 cgctgttgct tgtcgccgcc gcggatgccg cggcgcaggc catgccgggc gaggcgccgg
     1081 ccgccggatc gatgccctcg ctcgcgctcg gcctttcctc cggcggcgtg cgcgcgctga
     1141 acgaggagtt cgacgagcag cggcgcgccc tcgacgcttt cggcgggcgc accagcgtcg
     1201 atcgcttcac caacggcggc ttcgagaagc cccggcactg gacgctgtat gggcggctcg
     1261 gcgtgttcgg cttccaggac cagctcgatg ccgggcgctc ggacggcatc cagttcaagc
     1321 tgggccgcac cggcccgaag ctgaccgggc ggacttacgt cgggattcac cgcacgttct
     1381 gaacgtggca gaatcgaatc gtggcagacg ctaaggaaga ggatcccgtc acgccccgac
     1441 gctcgagctg gtcggccgcc ctggcgcagt tgcccggctt cccggacaag gttccgctgc
     1501 cgggcctgcc gccgcgcggc gcgcaggcgg tgtcgccgct gggcaaggac gccgacgaaa
     1561 cggacgagcc gggcacgccc gagcagtagc gggtgaacag cggcagggcc aggctcgtca
     1621 tcgcggcgct gatcgcgctt ttcgcgctgg tcggctattt caccaagacc accgtcaacc
     1681 cgatcaccgg ggagacgcag cgcgtggcgc tgacgcccga ggaggaggtc gccctcgggc
     1741 tgaaggcggc acccgagatg gcggcgcaga tgggcggatt gtcgcgcaac gagaaggccc
     1801 gcgcgctggt gcagcgcgtc ggcaccaagc tggcgagcgg cggcttcgcc gccaagagcc
     1861 cctacaagtt cagcttccat gtcctcgccg accccgagac ggtgaacgcc ttcgcgctgc
     1921 ccggcgggcc ggtgttcatc accgagggcc tgctgcgccg gctgcgcacg gagggcgagc
     1981 tcgcggcggt gctcggccac gaggtgggtc acgtgctagc gcggcattcg tccgagcggc
     2041 tcgccaagga gcagctcacc cagggcctga tcagcgcggt ggtggtgggc accggcgact
     2101 acacgagcgc ccagatcgcc cagatggcgg gcagcatgat caacatgaaa tacggccgcg
     2161 gcgacgagct ggaagccgac gcgctcggca tccgcatcgc gttcgagagc ggctacgacc
     2221 cgcgcgcgat gatcggggtg atggaggtgc tggcgcaggc ctccggcggc tcgaggcagc
     2281 cggaattcgc ctcgacgcac ccggcgccgg aaaaccgcca ggcacgcatc aaggaggaaa
     2341 tcggaaaacg tttcccctcg ggcgtgcccg acggcctgac caggtagcga gctacttcgt
     2401 agtgctcggc gtgctggggc tgctcggtgt gggggccggg gatcccatcc cggtcgagcc
     2461 ggagctggag cccgatcccg tgctgcctga cgagctcgat ccgctgctcg gcgtgctgct
     2521 cgagcccgaa ccggtgctgc tgcccgtgct gcccgagccg ctgctgctcg agttggcgcc
     2581 gtactgacgc acgcagtcgt cgcgctcgag gccggtcttg cccacgcact ggctgggcag
     2641 ggcgctgggg ctgctgctgt cgctggacga gctgctgctg cccgagctga tcgagcccga
     2701 accgctggtg ccggcgcctg cgctgccgcc ggcggcgggg ctggtcgtcg agctgccggg
     2761 gctggtcgcc gagctgcccg tgctgcccga ggtctgcgcc cacgaggcgc tcgccaagcc
     2821 gagcagcgct gcggccactg cggcactaag tgtctttacc atcacgatgt acgctccttt
     2881 ggttgggtaa gaagtagaga ctgcaggcgg aggccatgca atccggatgc cgcccggaaa
     2941 aaaacctgga gcaggtacgc aagctgcagg gcgtcaaccc ggcgtgaggg ctggacttcg
     3001 agccgctagg gccgctccag ccacgccacc agctcctgcg tgacgcgctc gggctgctcg
     3061 agggcgcaca tgtgcccgca atcggcgacg gtaacgagcc gcgccccggc gatgccctcg
     3121 gccatctcgc gcgcgcgatc cggcggggtg agctcgtcct gctcgccgac caggaccagg
     3181 ctggggcagc ggatcgcggc gagtgtcggg cgcgagtcgg gccggttcat gatcgcggtc
     3241 tgctggcgga tgaatgcctc cgcgcccgtt tcctccgcca tcagctcgat ttgccggcgc
     3301 agcgccgcgt cggcgcggcg cgaggcgtgc acgagcaccg gcagctgcag ttcgacgatc
     3361 gcctgcatgc ggccgccggc ggcgatcgcg atctggctgc gccggcgctc gctctgctcc
     3421 ggcgtgtccg gccgcgccgc ggtgtcgagc agcgcgagct tcactacccg cccgggggcc
     3481 tggcgcagga tctcgaaggc gacgtagccg cccatggaat ggccggcgag cgcgaagcgc
     3541 ggcggcgcgc tcgccaggat gcggcgcgcg atgccggcca tgctgtcgtc gcgcttgtga
     3601 tccgcgatgg tcaccgggcc gaaggcccac agcgccggca gctgcagctc gtagaaccgc
     3661 ggcgtggagt agaggcccgg caccaggacg atggcttgcg gctcgcgcat cagagcagcc
     3721 attctatcgg cagcaccgac aggaaccgga tccaggcgcg ccgcagcatg cccgcgccgg
     3781 gctcggtggt gtaacgcgtc tcgcccgccg ccgtgcgctc gatccattcc aggccgccgt
     3841 cggccgcgag ccgcacctcg tacgccgcgc gcggcagctt ctcgtcgagc acctcggcga
     3901 ggcgccgccc gagcaccggg ctgtcgatca tcagccccat ctcggtattc agcagggccg
     3961 agcgctggtc gaagttgaac gagccgacga agctgcggct gcggtcgagc gagaaagtct
     4021 tggcgtgcag gctggccgag gagctgcccg aagcgcgctt gcccctgggg gcgtcgcgct
     4081 ccgccgtggg cttgagctcg tagagccgca cgccggcgcg cagcagcgct tcgcgccgct
     4141 tcgcatagcc cgcgtgcacc gcgccgacgt cggtcgccgc gagcgagttg gtgaggacgc
     4201 gcaccgcgac gccgcgccgc gcgagcgcct ccaggccggc gctgccctcc ttgcccggca
     4261 cgaagtacgg cgagatgagg tcgaagcttt tttccgcgcg cgccgtgctg cgcagcagct
     4321 cggcgagcag cagcacgtcg gtgcgcccgg tcgtgtcgag cgtcttggcc gggtcgtcgc
     4381 gcagcaggcg cgcttcggcc cattccagcg ccagccggcc ggcgaggagc tcgctcacca
     4441 gcggcgtctc gcgcagcgcc ttgacgtagg ccgccgaccc gggatcggcg cgcgtcgcgg
     4501 cgaagtccgc ctcgagctgc gcggcggctt gcgggccggg cgcgctcagc agggacgcca
     4561 cgggataggc cgaggcgctg ttccagtaca ggtcgaaggc cttcgacacc tcgccgactg
     4621 ccggcccgac ggcgaggacg tcgagatcct cgaacagcgt ctcgcgcccg gcgccgaaat
     4681 actcgttgcc gacgttgcgc ccgccgacga tgctggcctg gctgtcggcg gtgaaggatt
     4741 tgttgtgcat gcgccgattg aggcgcgtga agtcggtcgc gtactggagc gcgcgcgcgc
     4801 ggcgctgcgc gaacgggttg tacaggcgca cctcgatgtt ggggtgcgcg tcgagcgcgg
     4861 caagtgtcgg gtcgaggccg gcgacgccgt tgtcgtcgag cagcaggcgc acgcgcacgc
     4921 cgcgctgcgc ggcgcggtgg atcgcttcga acagcagcca cccggtctgg tcgccgtgcc
     4981 agatgtagta ctggacatcg agcgagcggt ccgcggcggc ggcgagcagc acgcgcgccg
     5041 cgaacgcgtc gaccggttcc gccaaggcgt agatgcccgt cctggcggga tgcgcggcga
     5101 catggggcgc gacggcgcgc gcgagccttg tgccggccgt gtcctcgagc gcgaagctcg
     5161 cgctgcggcc ttccagcgac ggcaggccgc cgcaccccga gagcagcagg ccgagcggca
     5221 cgatgacgga cctcatgccc ccatcataga aggcgcggtg ctagactggg caacaggaga
     5281 ggagaccaaa tgaaactgca aaaaatcgcc gtcgtcgccg cggctgccgc ggccttcgcc
     5341 ggctgcagca ccatgaacga catgcgggcc gtgatgccgg gctcgatggg cggctggacc
     5401 acgctggtcg atggcggcaa ggggctggag aacttcgacc gcatcggcaa ttccaactgg
     5461 cgcgtcgagg acggcgccat cgtcgccgac cgcaacccgg acaagacgcc gagctacctg
     5521 gtcacgcgca cttcctactc gaacttcgtc atcaaggcgg agttctgggc gagcgacgac
     5581 gccaacagcg gcatcttctc gcgctgcaaa gatccgaaga acgtgggcga cgagacctgc
     5641 tacgaggcca acatctacga ccagcgcccc gacccgagct acggcaccgg cgcgatcgtc
     5701 aaggtggcca aggtcgaccc
//
LOCUS       DASYJA010000442         3576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1973070, whole genome shotgun sequence.
ACCESSION   DASYJA010000442 DASYJA010000000
VERSION     DASYJA010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1973070"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VGO02_09670"
     CDS             <1..440
                     /locus_tag="VGO02_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV7477989.1"
                     /translation="GPDVGVPELAKDPRVATIAERRKNQQLVWTLLNKFAEKYTKREL
                     MAILNPIDVPCGPIMSTQDLATDEHVRGRDMWVELDHPQRGKWWNVGMPIKLSASPTQ
                     IKRSPTLGEHTAEILKEVLGYDDARISNLKSAGAFSVPPKKAA"
     gene            437..1369
                     /locus_tag="VGO02_09675"
     CDS             437..1369
                     /locus_tag="VGO02_09675"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEV7477990.1"
                     /translation="MKIAIVGQQDFGKAVLEAFLARGDQVGAVFCAPEKEGARPDSLR
                     VAAQEKGLQVHQFKSLKAPEAAAAMKQARADIAIMAFVLQFAPQEFVNIPKKGTIQYH
                     PSLLPKYRGPSSINWPISRGEKETGLSIFRPTDGLDEGAVILQKRTAVGENDTLGSVY
                     FDRLFPMGVQAMLEAADLVLGNKHKEIPQDESQASYEGWFREAEAKIHWASHVDAIHN
                     LIRGADPAPGAWTTLNGVKLQLFDSRKELVRTFGAVKGKIGEVVEAAEGRLVITAQGG
                     RIHAGKAKLGDGKKVTAGDLISSGQIKAGTLLGS"
     gene            1523..>3576
                     /locus_tag="VGO02_09680"
     CDS             1523..>3576
                     /locus_tag="VGO02_09680"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV7477991.1"
                     /translation="MKSAFWKTDWFLGTVVVLAVLAFSKFSDLIPSLERKAYDLGVQA
                     TSRSASDKIAVIAIDDTSIANIGRWPWSREVHAKMTDLLAGAKAKVIVNTVFFSEAQV
                     DPGYAYVTKLLDLPAVKNEPPSAEMAPVLALLKEAEQSLNTDRKLAESYGKAGNVLLP
                     MLFQLGQPRGKPDRALPEFALKNAIKAQGSEDFPPLPSVAVQLPIEAVGRSAAALGHL
                     NAYPDVDGGVRTEPLVLQYYDQYFPSLSMMVAAKSLNLGAADINVKLGESVSLGRLKI
                     GTDTASQMYTYFYRNRGDGPAFQVDSFYDVLTGKIPATKYADKIVLVGPTAAGVGSTQ
                     VTPVSPAMAPVLTLAHSVSSILQEHFFTAPAWAYWGELAAWLLVAAYLIALLPRLKAG
                     PALVLSAAVFVALLATHFALMISAGVWMQLMLPATLLLVGHAALVSKRFIATEAGKQK
                     SDESSAESNRMLGLAYQGQGQLDLAWDKFRQVPLSDALLDNLYNLALDYERKRQFNKA
                     ESVFRYMHEFNPKFRDLEQKLSRAQKLSETVILGGGGSHPGGSLNLAVGEKPKFGRFE
                     VQKELGKGAMGVVYLGKDPKIGREVAIKTMALAQEFEADELAEVKERFFREAETAGRL
                     SHPNIVTIYDAGEEHDLCYIAMELLKGKDLAPFVKPGALLPMEKAASIVARVADALGY
                     AH"
BASE COUNT          673 a         1160 c         1192 g          551 t
ORIGIN      
        1 tcggccctga cgtaggcgtt cccgagctgg cgaaggaccc gcgcgttgcg acaatcgctg
       61 agcggcgcaa gaaccagcag ctggtatgga ccctgctcaa caagttcgcc gagaaatata
      121 ccaagcgcga gctgatggcg atcctgaacc cgatcgacgt tccgtgcggg ccgatcatgt
      181 cgacgcagga cctcgccacc gacgagcacg tgcgcggccg cgacatgtgg gtcgagctcg
      241 accacccgca gcgcggcaag tggtggaacg tcggcatgcc gatcaagctg tccgcctcgc
      301 cgacccagat caagcgctcg ccgacgctgg gcgagcacac cgcggaaata ctaaaggaag
      361 tgctcggtta cgacgatgca aggatctcca atctaaaaag cgcgggcgca tttagcgttc
      421 cacccaagaa agcagcatga aaatcgccat cgtcgggcag caggatttcg gcaaggcggt
      481 gctcgaggcg ttcctcgcgc gcggcgacca ggtcggcgcg gtgttctgcg cgccggagaa
      541 ggagggcgcg cggccagact cgctgcgcgt cgcggcgcag gagaagggcc tgcaagtgca
      601 ccagttcaag tcgctgaagg cgcccgaggc ggccgctgcg atgaagcagg cccgcgcaga
      661 catcgccatc atggccttcg tgctgcagtt cgcgccgcag gagttcgtca acatcccgaa
      721 gaagggcacg atccagtacc acccgtcgct gctgccgaag taccgcggcc cgtcgtcgat
      781 caactggccg atctcgcgcg gcgaaaagga aaccggcctc agcatcttcc ggccgaccga
      841 cggcctggac gagggcgcgg tgatcctgca gaagcgcact gcggtgggtg agaatgacac
      901 cctgggcagc gtctacttcg atcggctgtt ccccatgggg gtccaggcga tgctggaagc
      961 cgcagattta gtccttggca acaaacataa agaaatcccc caggacgagt cgcaggcctc
     1021 ctacgagggc tggttccgcg aggccgaggc gaagatccac tgggcgagcc atgtcgacgc
     1081 gatccacaac ctgatccgcg gcgccgaccc ggcgccgggg gcctggacca ccctgaacgg
     1141 ggtcaaattg caactgtttg attcccgcaa ggagttggtg cgcaccttcg gcgcggtcaa
     1201 gggcaaaatc ggcgaggtgg tcgaggcggc cgaggggcgg ctggtaatca ctgcgcaggg
     1261 cggccgcatc cacgccggca aggcgaagct cggcgatggc aaaaaagtca cagccgggga
     1321 ccttatctcc tcaggtcaaa tcaaagccgg gaccctgctc ggcagctagt tgtaaatctt
     1381 gcgtccgagc gaaacccccg ttcgcacgta aatatttgac ggagcttaca tttaactgca
     1441 aaaatgcggc ttaggccgct gttttttgtg gggagttgcc gcgcgtcagg gccgcgcgga
     1501 gctggcgaaa ggggaggggc gaatgaaatc cgcgttctgg aagaccgact ggttccttgg
     1561 aaccgtcgtg gtgctcgccg tcctcgcctt cagcaaattc agcgacctga ttcccagcct
     1621 cgagcgcaag gcctacgacc tgggcgtgca ggccacctcg cgcagcgcct ccgacaagat
     1681 cgcggtgatc gcgatcgacg acaccagcat cgccaacatc ggccgctggc cctggtcgcg
     1741 cgaggtgcac gccaagatga ccgatctgct cgccggcgcc aaggcgaagg tgatcgtcaa
     1801 cacggtgttc ttcagcgagg cgcaggtcga cccgggctac gcctacgtca ccaagctgct
     1861 cgacctgccc gcggtgaaga acgagccgcc gagcgccgag atggcgccgg tgctggcgct
     1921 gctgaaggaa gccgagcaga gcctcaacac cgaccgcaag ctcgccgaga gctacggcaa
     1981 ggccggcaac gtgctgctgc cgatgctgtt ccagctcggc cagccgcgcg gcaagcccga
     2041 ccgggcgctg cccgagttcg ccctgaagaa cgcgatcaag gcgcagggca gcgaggactt
     2101 cccgccgctg ccgagcgtcg cggtgcagct gccgatcgag gcggtcgggc gcagcgccgc
     2161 ggcgctgggc cacctgaacg cctacccgga cgtggacggc ggcgtgcgca ccgagccgct
     2221 ggtgctgcag tactacgacc agtacttccc gtcgctgtcg atgatggtcg cggcgaagag
     2281 cctcaacctc ggcgcggccg acatcaacgt gaagctcggc gagagcgtgt cgctcggcag
     2341 gctgaagatc ggcaccgata ccgcttcgca gatgtacacc tacttctacc gcaaccgcgg
     2401 cgacggcccg gccttccagg tcgactcgtt ctacgatgtc ctcaccggca agattccggc
     2461 caccaagtac gccgacaaga tcgtgctggt cgggccgacc gccgccggcg tcggcagcac
     2521 ccaggtcacg ccggtgtcgc cggccatggc gccggtgctg accctcgccc actcggtgtc
     2581 ctcgatcctg caggagcact tcttcaccgc gccggcgtgg gcctactggg gcgagctggc
     2641 ggcgtggctg ttggtggccg cctacctgat cgcgctgctg ccgcggctga aggcggggcc
     2701 cgccctagtg ctctcggccg cggtgttcgt cgcgctgctc gccacccact tcgcgctgat
     2761 gatctcggcc ggcgtctgga tgcagctcat gctgccggcg acgctgctgc tggtcgggca
     2821 cgccgcgctg gtgtcgaagc gcttcatcgc caccgaggcg ggcaagcaga agtccgacga
     2881 gagctcggcc gagtcgaacc gcatgctcgg cctcgcctac cagggccagg gccagctcga
     2941 cctcgcctgg gacaagttcc gccaggtgcc gctgtccgat gcgctgctcg acaacctcta
     3001 caacctggcg ctcgactacg agaggaagcg ccagttcaac aaggcggaga gcgtgttccg
     3061 ctacatgcac gagttcaacc cgaagttccg cgacctcgag cagaagctgt cgcgcgcgca
     3121 gaagctgtcc gagacggtga tcctcggcgg cggcggcagc cacccgggcg gctcgctcaa
     3181 cctggcggtc ggcgagaagc cgaagttcgg ccgcttcgag gtgcagaagg agctcggcaa
     3241 gggcgcgatg ggcgtggtgt acctcggcaa ggacccgaag atcggccgcg aggtggcgat
     3301 caagaccatg gcgctggcgc aggagttcga ggccgacgag ctggcagaag tgaaggaacg
     3361 ttttttccgc gaagccgaaa ccgccgggcg gctgtcgcat ccgaatatcg tcaccattta
     3421 cgacgcgggc gaagagcacg acctgtgcta catcgccatg gaattgttga agggcaagga
     3481 cctggccccg ttcgtcaagc cgggtgccct gcttccaatg gaaaaggccg catcgattgt
     3541 cgcgcgcgtg gcggatgccc tgggctatgc gcacaa
//
LOCUS       DASYJA010000443         1595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_885720, whole genome shotgun sequence.
ACCESSION   DASYJA010000443 DASYJA010000000
VERSION     DASYJA010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885720"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VGO02_09685"
     CDS             <1..201
                     /locus_tag="VGO02_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477992.1"
                     /translation="PHLANPHFTLQASAWYQHLPQYWPPQYLSGRDQALRNAPRERAE
                     QTELRIKARPETHEVKDTKKAA"
     gene            198..881
                     /locus_tag="VGO02_09690"
     CDS             198..881
                     /locus_tag="VGO02_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEV7477993.1"
                     /translation="MITLWELGGKDARRYSLFSWRTRMALMHKGLAYETQPVCMSDKP
                     AIAFSNGKTVPVIKDGETVVRESWKIAEHLEDRYPQAPALFGGAIGRGVSQAFNTWVD
                     RAVVPAMLPVIVADIHERVDPRDDGFFRDMMEKIVKKTLEQTRAERDDALKRLGRALE
                     PMQAALKRQSYVCGREPAYADYVLFSVFQWARVMSPQEVLGPEDPLCGWRERVLDLFG
                     GFARNVPTA"
     gene            865..1395
                     /locus_tag="VGO02_09695"
     CDS             865..1395
                     /locus_tag="VGO02_09695"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV7477994.1"
                     /translation="MSRRPERKRCRWPGNPLAIAYHDKEWGVPVHNDRKLFEFLILEG
                     AQAGLSWDTILAKRENYCKVLHDFDFEKISRYTVKDKRRLLANPGIVRNRAKIDATIV
                     NARCFIALRKEFGSFSRYVWGFAGKSAEALSKDLRKRGFNFVGPTIMYAFMQAVGMVN
                     DHEKTCFRWRELNAKN"
     gene            1406..>1595
                     /locus_tag="VGO02_09700"
     CDS             1406..>1595
                     /locus_tag="VGO02_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7477995.1"
                     /translation="MLLALAASPAFAAYDANGVALGGSEDDVKRKFPSVRCQPLEWKS
                     KAADRRCDDAKIVFAGGVP"
BASE COUNT          297 a          501 c          523 g          274 t
ORIGIN      
        1 ccacaccttg ccaacccgca tttcacgctg caggcttcgg cgtggtacca gcacctgccg
       61 cagtactggc cgccgcagta tctttcgggt cgcgaccagg cattgcgcaa cgcgccgcgc
      121 gagcgcgccg agcagacgga gctgcgcatc aaggcgcgcc cggaaaccca cgaggtcaag
      181 gacaccaaga aggcagcgtg attactctct gggaactggg cggcaaggac gccaggcgct
      241 acagcctgtt ctcctggcgc acgcgcatgg cgctcatgca caagggtctc gcgtatgaga
      301 cgcaacccgt ttgcatgagc gataagccgg caatcgcctt ttcgaacggc aagaccgttc
      361 cggtcatcaa ggacggagaa accgtggtgc gcgagtcctg gaagattgcc gagcatctcg
      421 aggaccgtta tccgcaggcg ccggcgctgt tcggcggcgc catcggtcgt ggcgtgtcgc
      481 aggccttcaa cacctgggtc gaccgcgcgg tggtgccggc gatgctgccg gtgatcgtcg
      541 ccgacatcca cgagcgggtc gatccgcgcg acgacggctt tttccgcgac atgatggaga
      601 agatcgtgaa gaagaccctc gagcagacgc gcgccgagcg cgacgacgcg ctcaagcgcc
      661 tggggcgcgc gctcgagccg atgcaggcgg cgctcaagcg ccagtcctac gtctgcgggc
      721 gcgagccggc ctacgccgac tacgtcctgt tctcggtctt ccagtgggcg cgcgtcatga
      781 gcccgcaaga ggtgctcggc ccggaggacc cgctgtgcgg ctggcgcgag cgcgtgctcg
      841 atctcttcgg cggcttcgcg cgcaatgtcc cgacggcctg agcggaaaag atgtcgatgg
      901 ccgggcaatc cgctcgccat cgcctatcac gacaaagagt ggggcgtgcc ggtccacaac
      961 gaccgcaagc tcttcgagtt cctgatattg gagggtgcgc aggccggcct gtcgtgggac
     1021 accatcctcg ccaagcgcga aaactactgc aaggtcctgc acgattttga ttttgaaaaa
     1081 atttccagat acaccgtaaa agacaaacgc cgtctgctgg ctaacccggg catcgttcgc
     1141 aatcgcgcca agatcgatgc caccatcgtc aacgcccgct gcttcattgc gttgaggaag
     1201 gagttcggca gtttttcgcg ctatgtctgg ggcttcgccg gcaagagcgc cgaggcgctg
     1261 tcgaaggacc tgaggaagcg cggcttcaac ttcgtcgggc cgactatcat gtacgctttc
     1321 atgcaggcgg tgggcatggt gaacgaccac gagaaaacct gttttcgctg gcgggagctg
     1381 aatgcgaaaa actgaactgg cggttgtgct gctggcgctt gctgcgagcc cggcttttgc
     1441 cgcctatgac gccaacggcg tcgcgctcgg cggcagcgag gacgacgtga agcgcaagtt
     1501 ccctagcgtg cgctgccagc cgctcgagtg gaagagcaag gccgccgacc ggcgctgcga
     1561 cgatgccaag atcgtcttcg ccggcggcgt gccgg
//
LOCUS       DASYJA010000444         3226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1790930, whole genome shotgun sequence.
ACCESSION   DASYJA010000444 DASYJA010000000
VERSION     DASYJA010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1790930"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1625)
                     /gene="boxC"
                     /locus_tag="VGO02_09705"
     CDS             complement(45..1625)
                     /gene="boxC"
                     /locus_tag="VGO02_09705"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HEV7477996.1"
                     /translation="MISFETDPSRYNHWKLGFDGPLATLTLDVAEDKPLVPGYKLKLN
                     SYDLGVDIELADALNRVRFEHPEVKAVVLTSGKSRMFCSGANIYMLGHSSHAWKVNFC
                     KFTNETRNGIEDASQHSGLKFLAALNGTTAGGGYELALACDEIAMIDDRSSTVSLPEV
                     PLLGVLPGTGGLTRLVDKRKVRRDLADVFCTTAEGVRADRAREWKLIDHTAKPQQFAD
                     AVKARALELAKLSDRPGGQGVALTPLGENRIISVKLDEASRTAEILVKGPQGGSMEKS
                     AEWYPLRLARELDRAILDLRQNQPDIGLWVLKTQGDIASVLQLDDLLSKNQNDWFVRE
                     TVGFLRRVFSRLDVSSRSIFAIVEPGSCFAGTLAELMFAADRSYMLEITGSEMSGPRI
                     ALSEINFGAYPMASGISRLQAHYCGEVPAIEQQKLYSTEDARKIGLVTATPDDIDWED
                     EVRLAIEERTSLSPDALTGMEASLRFTGAETMQTRVFGRLTAWQNWIFIRPNAVGEQG
                     ALKVYGTGARAKFNWERV"
     gene            complement(1622..2383)
                     /locus_tag="VGO02_09710"
     CDS             complement(1622..2383)
                     /locus_tag="VGO02_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV7477997.1"
                     /translation="MNAPLTPAQQESAYLARLGERVRAWRMEHGTTRKVLAQSSGVSE
                     RYLAQLEAGEGNISVLLLRKVARAMSVPVEQLVREEERAPGAERIALLGLRGAGKSTL
                     GKKLAESLGMPFVELDLEVEKEAGAKLGEVFAMYGQDAYRRFERRALERVLRETPRAV
                     IAAGGSLVTDPGTYDLLLERCRCVWLKTSPEEHMSRVIAQGDMRPFKGRSAALAEIRK
                     LLEDRDKLYARAGLAIDTSGKSARQSLDQLKKALT"
     gene            2433..2903
                     /locus_tag="VGO02_09715"
     CDS             2433..2903
                     /locus_tag="VGO02_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEV7477998.1"
                     /translation="MRINILVGTMTGTAQLCAQEMELALGDDDVRIETILMDSLDSSV
                     FEREGVFLVCTSTYGQGDVPDNAKALYEDLKGKRPDLSRVRYGVFGLGDRTYAETFNF
                     GGKRFDDILGELGAERIGERHTHDASSGILPEETALEWGQDWLRRVRERLAQPA"
     gene            2947..>3226
                     /locus_tag="VGO02_09720"
     CDS             2947..>3226
                     /locus_tag="VGO02_09720"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEV7477999.1"
                     /translation="MRALKWVGWLLLALAVLFTILVFSLGQFKGPISRAVSKATGREL
                     LIEGDLRVVPSLVHPRFRAERVTFANADWGQYDYLLQADAIEATVNLLG"
BASE COUNT          511 a         1021 c         1085 g          609 t
ORIGIN      
        1 caggttgacg ttgttcggga tcttttccga ataattgatt ccgctcatac gcgctcccaa
       61 ttgaatttgg ctctcgcgcc ggtgccgtaa accttgaggg caccttgctc gccgaccgca
      121 ttcggtcgga tgaaaatcca gttctgccac gccgttagcc gcccgaaaac gcgcgtctgc
      181 atcgtctccg caccggtgaa gcgcaggctc gcctccatcc cggtcagcgc atcgggcgag
      241 agactggtgc gctcctcgat cgccaggcgc acttcgtctt cccagtcgat gtcgtccggg
      301 gtggcggtca cgagcccgat ttttctcgcg tcttcggttg aatagagctt ttgctgctcg
      361 atcgccggga cttcgccgca gtaatgcgcc tgcagcctgg aaatcccgct ggccatcggg
      421 taggcgccga agtttatttc ggagagcgcg atccgaggac cgctcatttc acttcctgtg
      481 atctcgagca tgtagctgcg gtcggcggcg aacatcagct cggcgagcgt cccggcgaag
      541 cacgagcccg gctcgacgat ggcgaagatc gagcgcgagg agacgtccag gcgcgagaag
      601 acgcggcgca ggaagccgac ggtctcgcgc acgaaccaat cattctggtt ttttgaaaga
      661 agatcatcga gttgcaggac tgaagcgata tcaccctgcg tcttcaaaac ccagaggccg
      721 atatccggct ggttctggcg caggtcgagg atggcgcggt cgagctcgcg ggcaaggcgc
      781 agcggatacc actccgcgct cttttccata ctgccgccct gcggtccctt cacgagaatc
      841 tcggcagtcc tcgatgcttc gtccaatttc accgaaataa ttcggttctc gccgagcggt
      901 gttagtgcaa cgccctggcc gccggggcgg tcggagagct tggcgagctc gagcgcgcgt
      961 gccttgacgg cgtcggcgaa ctgctgcggc ttcgccgtgt ggtcgatgag cttccattcc
     1021 ctggcgcggt cggcgcgcac gccctcggcg gtggtgcaga agacgtcggc aaggtcgcgt
     1081 ctgacttttc tcttgtcgac caatcttgtc aacccgcccg tgccgggcaa aacccccagc
     1141 agcggcacct cgggcaggct tacggtcgag gagcggtcgt cgatcatcgc gatctcgtcg
     1201 caggcgaggg ccagctcgta gccgccgccg gcggtggtgc cgttcagcgc ggcgaggaac
     1261 ttgaggccgg agtgctggct cgcgtcctcg atgccgttgc gcgtctcgtt ggtgaacttg
     1321 cagaagttca ccttccaggc gtggctcgag tggccgagca tgtagatgtt ggcgcccgag
     1381 cagaacatgc ggctcttgcc gctggtgagc accaccgcct tgacctcggg atgctcgaag
     1441 cgcacgcggt tcagcgcgtc ggcgagctcg atgtcgacgc ccaggtcgta gctgttgagc
     1501 ttcagcttgt agccgggcac caggggcttg tcctcggcga cgtcgagggt gagggtggcg
     1561 agcggcccgt cgaagccgag cttccagtgg ttgtagcgcg agggatcggt ctcgaagctg
     1621 atcatgtgag ggctttcttc agctggtcca ggctctgtct tgcggatttg ccggaggtgt
     1681 cgatggccag gccggcgcgg gcgtacagct tgtcgcggtc ttccagcagc ttcctgattt
     1741 ccgccagcgc ggcggagcgg cccttgaacg gccgcatgtc gccttgggcg atgacgcggg
     1801 acatgtgctc ttcgggagag gtcttgagcc agacgcagcg gcagcgctcg agcagcagat
     1861 cgtaggtgcc ggggtcggtg accaggctgc cgccggcggc gatgaccgcg cgcggcgtct
     1921 cgcgcagcac gcgctccagg gcgcggcgct cgaagcggcg gtaggcgtcc tggccgtaca
     1981 tggcgaacac ttcgccgagc ttcgcgccgg cttccttctc gacctcgagg tcgagctcga
     2041 cgaacggcat gcccaggctt tcggcgagct ttttgccgag ggtcgatttt ccggcgccgc
     2101 gcaatcccag cagggcgatg cgctcggcgc ccggggcgcg ctcttcttcg cgcaccagct
     2161 gctcgaccgg gacgctcatg gcacgcgcga ctttgcgaag cagcaggacc gagatgttgc
     2221 cctcgccggc ctcgagctgc gccaggtagc gctccgagac gccggaggac tgcgccagca
     2281 ctttgcgcgt cgtgccgtgc tccatccgcc aggcccgcac gcgctcgccg aggcgggcga
     2341 ggtaggcgct ttcctgctga gcgggggtta gcggagcatt catgaagtat agtgcttgac
     2401 gtggattcta gtctgcacta tattgactgt caatgagaat caacatcctg gtcggcacga
     2461 tgaccggcac cgcacaactg tgcgcccagg agatggagct cgcgctggga gacgacgacg
     2521 tccggatcga gacgatcctc atggacagcc tcgacagctc ggtcttcgag cgcgaaggcg
     2581 tgttcctggt ctgcacctcg acctacggcc agggtgacgt tccggacaac gccaaggcgc
     2641 tctacgagga cctgaagggc aagcgccccg acctatcgcg cgtgcgctac ggcgtgttcg
     2701 gcctgggcga tcgcacctac gccgagacct tcaacttcgg cggcaagcgc ttcgacgaca
     2761 ttcttggcga gctcggcgca gagcgcatcg gcgagcgcca tacgcacgat gcctcgagcg
     2821 gcatcctgcc ggaggaaacg gcgttggagt ggggccagga ctggttgcgc cgcgtgcgcg
     2881 aaaggctggc ccagccggcc taggcagcag cggaaacggc ggcacgccgt ttgcactcca
     2941 agtggtgtgc gcgccctcaa gtgggtcggc tggctgctcc tggcgctggc cgtcctgttt
     3001 accatcctgg ttttctcgct cggccagttc aaggggccga tctcgcgcgc cgtcagcaag
     3061 gcgaccgggc gcgagctgct gatcgagggc gacctgcggg tcgtgcccag cctggtccac
     3121 ccgcgcttcc gcgccgagcg ggtcaccttc gcgaatgccg actgggggca gtacgactac
     3181 ctgctccaag ctgacgcgat cgaagcgacg gtcaacctcc tggggc
//
LOCUS       DASYJA010000445         4354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2882493, whole genome shotgun sequence.
ACCESSION   DASYJA010000445 DASYJA010000000
VERSION     DASYJA010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2882493"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="VGO02_09725"
     CDS             complement(<1..840)
                     /locus_tag="VGO02_09725"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV7478000.1"
                     /translation="MFSSEARLAKHAIAAVAAFIVAFAAWAQPLPVAKPETVGMSSQK
                     LAKISQALKKEVDDGSFRGAVVMVARKGKLVYQDAIGMQTASAKMTRDSMFRIYSMTK
                     PLVSVAAMMLVEDGTIQLTDPVSKYLPGFDKLQVSVPGKNDAGQTTYTLVPQERQMTV
                     QDLLRHTSGLAYGEITQNAPVKEGLQKIGVYYPDFDFNARNLAPADQVAKMATVPLAF
                     QPGTTWHYSLASDMLGRVVERASGKRLGDFLQERLFAPLKMVDSGFWVPAPKMPRLAE
                     SLEK"
     gene            complement(869..1207)
                     /locus_tag="VGO02_09730"
     CDS             complement(869..1207)
                     /locus_tag="VGO02_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478001.1"
                     /translation="MTTERLIFTLISVPVLLGALFLLYAAPVRAVSALASSNSREVRV
                     PAMWLLGAWAVFVAMLAAYFVSGWWYRENIVIRPVDPLEVFALLMVLVFGIHLPSVAY
                     GRLAKARKTR"
     gene            complement(1204..1500)
                     /locus_tag="VGO02_09735"
     CDS             complement(1204..1500)
                     /locus_tag="VGO02_09735"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV7478002.1"
                     /translation="MPAPKAAKWWLYVLECHSGLLYTGIAKDVDARFAAHRNGTGAMF
                     TRLNRPVAILGKVSLRTRGQALRAEYALKQLSRADKLRWCAAGLRAFVRTSPAP"
     gene            complement(1487..2353)
                     /gene="ylqF"
                     /locus_tag="VGO02_09740"
     CDS             complement(1487..2353)
                     /gene="ylqF"
                     /locus_tag="VGO02_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEV7478003.1"
                     /translation="MSVNWFPGHMDTARKEAAKTMAKIDVVIEILDARLPGASSNPMI
                     ERLRLHRARPCLKVLNKSDLADPNVTAAWVRHFGKDKTVKAVALSCKKPAEVARIPAL
                     AASLAPHRDGGVKSLRMMVMGVPNAGKSTFINALLKRKVAKTGDEPAITRQQSRFQLG
                     NDMELIDTPGLMWPSIKHPSDGLILAASHVIGENAFISEEVARFLAEVLLERYPGSIE
                     KRYQFRTNTLDGPGLIEAIARKRGYLLKGGRVDYEKAGNALVHDYRSGALGRISLETP
                     ESRAAMLSHAGA"
     gene            complement(2350..2892)
                     /locus_tag="VGO02_09745"
     CDS             complement(2350..2892)
                     /locus_tag="VGO02_09745"
                     /inference="COORDINATES: protein motif:HMM:NF018813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HEV7478004.1"
                     /translation="MALGATIYHFTIRLSDVDRGVYETLELKVAKHPSESDEYLVTRV
                     LAYCLEYAEGIAFSRGLSSPDEPPIAVRDLTGVLKAWIEVGSPDAARLHKAGKAAPRV
                     AVYTHKDPAQLLRSLDGERIHRVEALELYSLGRELVAALVARLERRTALELSVSDRHL
                     YATIGGETLAGEVERLMMTA"
     gene            complement(2894..3295)
                     /locus_tag="VGO02_09750"
     CDS             complement(2894..3295)
                     /locus_tag="VGO02_09750"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEV7478005.1"
                     /translation="MITGGCLCGRVRYEARGEALFGVVCHCRDCQRASGAGHMPVIGF
                     RKSNFSLTGETRSYAVVGGSGKLAVRHFCPNCGSMLLGMPEIAPDLVTLYAGSLDEPY
                     EFKPDYVQFRRDCRDWDGLRFDVPQHHAAGQ"
     gene            complement(3292..3657)
                     /locus_tag="VGO02_09755"
     CDS             complement(3292..3657)
                     /locus_tag="VGO02_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478006.1"
                     /translation="MKSLLPELEHGAQPVIPRVQFAVRLAHSGVIALVLIAISLSIGM
                     IGYHALEGLNWLDSFLNAAMLLGGMGPVNAPVTYAGKLFAGLYALYCGLAVLLVAGVL
                     LAPVAHRILHRFHVESRSK"
     gene            complement(3654..>4354)
                     /locus_tag="VGO02_09760"
     CDS             complement(3654..>4354)
                     /locus_tag="VGO02_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV7478007.1"
                     /translation="WTLARDTTLGLNFVGINYYDGQGFMVQKKLKVKSAKQLNGATIC
                     MQPGTTTELNLADYFRANKMTFKPVVIEKLEEVLNAYFNGRCDVFTTDHSGLIALRAS
                     RAQKPDDHVILPEIISKEPLGPAVRHGDDRWFDVVKWSMFAMIEAEELGLSSKNIDKE
                     ASSINPTVQRFVGGSGDIGKMLGLDNKWAFNIVRQVGNYAESFDANLKPLGFERGLNR
                     LWNQGGLMYAPPIR"
BASE COUNT          713 a         1431 c         1435 g          775 t
ORIGIN      
        1 cttctccagc gattcggcga ggcgcggcat cttcggcgcc ggcacccaga acccggagtc
       61 caccatcttg agcggtgcaa acaggcgctc ctgcaggaag tcgcccaggc gcttgccgga
      121 ggcgcgctcc accacgcggc cgagcatgtc cgaggcgagg ctgtagtgcc aggtcgtgcc
      181 cggctggaat gcgagcggca cggtggccat cttcgcgacc tggtcggccg gcgccaggtt
      241 gcgcgcgttg aaatcgaagt ccgggtaata gacgccgatc ttctgcagcc cctccttcac
      301 cggcgcgttc tgcgtgatct cgccgtaggc gaggcccgag gtgtggcgca acaggtcctg
      361 caccgtcatc tggcgctcct gcggcaccag tgtgtaggtg gtctggcccg catcgttctt
      421 tccgggcacg ctcacctgca gcttgtcgaa gcccggcagg tacttgctca ccgggtcggt
      481 gagctggatc gtgccgtcct cgaccagcat catcgccgcc accgagacca gcggcttggt
      541 catcgagtag atgcggaaca tcgagtcgcg cgtcatcttg gccgacgcgg tctgcatgcc
      601 gatcgcgtcc tggtagacca gcttgccctt cctcgccacc atgaccacgg cgccgcggaa
      661 gctgccgtcg tcgacttctt ttttcagggc ctggctgatc ttcgccagct tctgcgacga
      721 catgccgacc gtctcgggct tggcgaccgg cagcggctgc gcccaggctg cgaatgcaac
      781 gatgaatgcc gcgaccgcgg cgatggcgtg cttcgccaga cgagcttcgg acgaaaacat
      841 ggtggtccct cctctaggga cttccacctt agcgtgtctt gcgggccttg gccagccgcc
      901 catacgcaac gctgggcagg tggatgccga aaaccaggac catgagcagg gcgaagacct
      961 cgagcggatc caccggccgg atgacgatgt tctcccggta ccaccatccg ctcacgaaat
     1021 aggccgccag catggcgaca aaaacggccc atgcccccag cagccacatc gcgggtaccc
     1081 gcacctcccg gctgttcgaa gacgcgagcg cggacaccgc ccgcacaggc gcagcgtaga
     1141 gaaggaacaa ggcccctagg agcaccggca ccgaaatcag cgtgaagatc aggcgttctg
     1201 tcgtcatggt gctggtgatg ttcgcacaaa ggcgcgcagc ccggccgcgc accagcgcag
     1261 cttgtcggcg cgggacagct gcttgagcgc gtattcggcg cgcagcgcct gtccacgggt
     1321 acgcagtgac accttgccga gaatcgcaac cggccggttc agccgggtga acatcgcgcc
     1381 ggtcccgttg cggtgcgctg caaagcgcgc gtcgacgtct ttcgcgatgc cggtgtagag
     1441 aagcccgctg tggcattcca gcacgtagag ccaccacttc gcagccttag gcgccggcat
     1501 gcgacagcat cgcggcgcgg ctctcgggag tctcgaggct gatacggccc agcgcaccgc
     1561 tgcggtagtc gtgcaccagc gcattgccgg ccttctcgta gtcgacgcgt ccgcccttga
     1621 gcaggtagcc gcgcttgcgc gcgatcgctt cgatgaggcc tgggccatcc aaagtatttg
     1681 ttctgaattg atacctcttt tcgatcgagc ccggatagcg ttcgagcaat acctcggcga
     1741 ggaacctggc cacttcctcg ctgatgaagg cgttctcgcc gatgacgtgg ctcgcggcga
     1801 ggatcaggcc gtcgctggga tgcttgatcg acggccacat gaggccgggc gtgtcgatga
     1861 gctccatgtc gttgccgagc tgaaaccggc tctgctgccg ggtgatggcc ggctcatcgc
     1921 cggtcttggc caccttgcgc ttgagcagcg cattgatgaa ggtcgacttg ccggcgttcg
     1981 gcacgcccat caccatcatc cgcagggact tcactccgcc gtcgcgatgc ggcgcgaggc
     2041 tcgccgccag cgccggaatc ctggcgacct ctgctggctt cttgcacgac agcgccacgg
     2101 ccttcaccgt cttgtccttg ccgaagtggc gcacccaggc ggccgtgaca ttgggatcgg
     2161 cgagatccga cttgttcagc accttcaggc acgggcgcgc ccggtgcagg cgcagccgct
     2221 cgatcatggg attgctgctc gcgccgggca ggcgcgcgtc gaggatctcg atcaccacgt
     2281 cgatcttcgc catggtcttg gccgcttcct tgcgcgccgt gtccatgtgg cccggaaacc
     2341 agttgacgct catgccgtca tcatgagcct ctccacctcg ccggccaagg tttcgccgcc
     2401 gatggtcgcg tagaggtggc ggtcgctgac cgacagctcg agcgcggtgc gccgctcgag
     2461 gcgcgcgacc agcgccgcga ccagctcgcg gccgagcgag tagagctcga gggcctcgac
     2521 gcggtggatg cgctcgccat ccagcgagcg cagcagctgc gccggatcct tgtgcgtgta
     2581 caccgcgaca cgcggcgccg ccttgcccgc cttgtgcagc cgcgctgcgt ccggcgagcc
     2641 gacctcgatc caggccttga gcacgccggt caggtcgcgc accgcgatcg gcggctcgtc
     2701 gggcgacgac agcccgcgcg agaaggcgat gccttcggcg tactccaggc agtacgccag
     2761 cacccgcgtc accaagtatt cgtcgctctc cgacggatgc ttggccacct tgagctccag
     2821 cgtctcgtag acgccgcggt cgacgtcgct cagccggatg gtgaagtggt agatcgtcgc
     2881 gccgagcgcc acgtcactga cctgccgcgt gatgctgcgg aacgtcgaat cgcagcccgt
     2941 cccagtcgcg gcagtcgcgc ctgaactgca cgtaatcggg cttgaactcg tacggctcgt
     3001 ccaggctacc ggcgtagagg gtgacgaggt cgggagcgat ttccggcatg ccgagcaaca
     3061 tgctgccgca gttcgggcag aagtggcgga ccgccagctt gccgctaccg cccaccacgg
     3121 cgtagctccg cgtttcgccg gtcaggctga aattcgattt gcggaatccg atgacgggca
     3181 tgtgtccggc gcccgaggcc ctttggcagt cccggcaatg gcataccacg ccaaacagcg
     3241 cctcgccgcg cgcttcatac cgcacgcgtc cgcacaggca tccgccggta atcacttgct
     3301 tcggctctcc acgtgaaacc ggtggaggat gcggtgcgcg acgggcgcga ggaggacgcc
     3361 ggcgacgagg aggaccgcca ggccgcagta aagggcatag aggcccgcaa agagcttgcc
     3421 ggcgtaggta accggcgcat tcacgggacc catcccgccc aggagcatcg ccgcgttcag
     3481 gaacgaatcg agccagttca gcccctcgag cgcgtggtaa ccgatcatgc cgatcgacag
     3541 tgaaatcgca atcagcacca gcgcgataac gccgctgtgg gcgagccgca cagcgaactg
     3601 cacccgggga attacgggct gggcgccatg ctcgagctcc ggcagcaggc ttttcatctg
     3661 attgggggcg cgtacatcag gccgccctgg ttccagaggc ggttgaggcc gcgctcgaag
     3721 ccgaggggct tgaggttggc gtcgaagctc tcggcgtagt tgccgacctg tcttacgatg
     3781 ttgaacgccc acttgttgtc gaggccgagc atcttgccga tgtcgccgct gccgccgacg
     3841 aagcgctgca cggtcgggtt gatgctcgag gcttccttgt cgatgttctt cgatgagagg
     3901 ccgagctcct cggcttcgat catcgcgaac atggaccact tgacgacgtc gaaccagcgg
     3961 tcgtcgccgt ggcgcacggc ggggccgagc ggctccttgg agatgatctc gggcaggatg
     4021 acgtggtcgt cgggcttttg cgcgcgcgag gcgcgcagcg cgatcagccc ggagtggtcg
     4081 gtggtaaaga cgtcgcagcg gccgttgaag taggcgttga ggacttcctc gagcttctcg
     4141 atcaccaccg gcttgaaggt catcttgttg gcgcggaagt agtcggcgag gttgagctcg
     4201 gtggtggtgc cgggctgcat gcagatcgtg gcgccgttca gctgcttggc gcttttcacc
     4261 ttcagcttct tctgcaccat gaagccctgg ccgtcgtagt agttgatgcc gacgaaattg
     4321 aggccgagcg tggtgtcgcg cgccagggtc cagg
//
LOCUS       DASYJA010000446         2981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2427140, whole genome shotgun sequence.
ACCESSION   DASYJA010000446 DASYJA010000000
VERSION     DASYJA010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2427140"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VGO02_09765"
     CDS             <1..748
                     /locus_tag="VGO02_09765"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEV7478008.1"
                     /translation="VAAEQVARATPDGHTLMLGDSGALAINVALNPKLSYQPLKDFTL
                     ITALAAVPTVLVVNPGVSAHSLQEFVSLGKSKSLAYGSAGNGSVHHLTMAVFLARAHV
                     EMLHVPYKGGTALVAAVLGGEVQSGWSGIPNVAAHIRSGRLRVLCISTARRSASLPEV
                     ATAIELGYPGFDIATTIGLQAPAGLPREVTARLQAAVAKALREPDLAERMANLGMELA
                     ENGTDHYARFVRDDLERFAAAVKTAGVKHE"
     gene            741..1889
                     /locus_tag="VGO02_09770"
     CDS             741..1889
                     /locus_tag="VGO02_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEV7478009.1"
                     /translation="MSELDRVRTDVVGSLLRPAAWKEARLRFEAGLMPAHEFALMEDG
                     FVKANLALQEAVGLDVVTDGELSRLNFQDSFGLSVSGYDTARETVATQERRAAGGAPL
                     SRWEIPDLAAPGTPVVHRRPVKERLQLTRNVPLEEYLRASPHARKPVKVTLVGPDRIA
                     QRFDHAASRNVYPSIDDFMADVVAVQRQMIAQLAAAGCRYVHIDAPGYTAYVDPPSLA
                     AMRARGEDPEATFSRSLAADAAVVAGFPGVTFGIHLCRGNQRSMWHREGAYDAIAERL
                     FGELPHQRFLLEYDSPRAGSFAPLRFVPRGRTVVLGLVSSKVPELEPLDEIRRRVDEA
                     ARLVPLEQLALSPQCGFGSDAVGNLVSEDDQKRKLERVVEAARTIWGK"
     gene            1886..2914
                     /locus_tag="VGO02_09775"
     CDS             1886..2914
                     /locus_tag="VGO02_09775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7478010.1"
                     /translation="MMQFGIFDWVEANDALAPADIYEHKLQLAAAADKAGFHGMFFAE
                     HQGTPLSIDASPAVLLAALFQRTQRLRAGALTFCLPWYDPYRFYNEVCMLDQLSGGRL
                     ELGVGRGVSPIESLIFGLTSIEQSRERYRETLDAFFAACSSPLLNFKSQRFEYRDVEL
                     FNKPVQRPYPPLWFPSSNRDSIEFTARHGYHTALIAKTVESKALFNQYREVWEKHKGD
                     QERHNAHVAAPFLAKTQHIVIAETYVEAEKIGLQAYESWKGHMHHLTRKYDRPEAMKL
                     EPFGEDSVQRLIVGPPNTVLEKLQEAIDATGMNYLLCIFSFGNLPPQAALRSLEMFAD
                     EVMPKLRD"
BASE COUNT          478 a         1053 c          986 g          464 t
ORIGIN      
        1 ggtcgccgcc gagcaggtgg cgcgcgcgac gccggacggc cacaccctga tgctcggcga
       61 ctcgggcgca ctcgccatca acgtcgccct gaaccccaag ctctcctatc agccgctcaa
      121 ggatttcacg ctcatcaccg ccctcgccgc cgtgccgacg gtgctggtgg tcaatccggg
      181 cgtaagcgcg cattcgctgc aggaatttgt ttctcttgga aaatcgaaga gtctcgccta
      241 cggctcagcc ggcaacggct cggttcacca cctcaccatg gcggtgttcc tcgcgcgtgc
      301 gcacgtggaa atgctccacg tgccctacaa aggcgggaca gcgctggtcg ccgcggtgct
      361 ggggggcgaa gtgcagtcgg gctggtcggg gataccgaac gtcgcggcgc acatccgctc
      421 gggcaggctg cgcgtgctgt gcatcagcac ggcgcggcgc tcggcctcgc tgcccgaggt
      481 ggccaccgcg atcgagctgg gctatcccgg cttcgacatc gccaccacca tcgggctgca
      541 ggcgcccgcc gggctgccgc gcgaggtcac cgcgcgcctg caggcggcgg tggcgaaggc
      601 gctgcgcgag cccgacctcg ccgagcgcat ggccaacctc ggcatggagc tggcggaaaa
      661 tggaaccgac cactacgcgc gcttcgtgcg cgacgatctg gagcggttcg ccgccgcggt
      721 gaagaccgcc ggggtcaagc atgagtgagc tcgaccgggt gcgtaccgac gtcgtcggca
      781 gcctgctgcg cccggcggcg tggaaggagg cccgcctgcg cttcgaagcg ggtctgatgc
      841 ccgcccatga gtttgcgctg atggaagatg gttttgtaaa agccaacctg gccctgcagg
      901 aagccgtggg tctcgacgtg gtcaccgacg gcgagctgag ccggctcaat ttccaggaca
      961 gcttcgggtt gtcggtgagc ggctatgaca ccgcgcgcga aacggtggcg acgcaggagc
     1021 ggcgcgccgc ggggggcgcg cccctcagcc gctgggagat cccggatctc gccgcccccg
     1081 gcacgccggt cgtgcaccgc cgcccggtga aggaaaggct gcagctgacc cgcaacgtgc
     1141 cgctcgagga atacctgcgc gcgtcgccgc acgcaagaaa acctgtaaaa gtcacgctgg
     1201 tcggccccga ccgcatcgcg cagcgcttcg accatgccgc ctcgcgcaac gtctacccct
     1261 cgatcgacga cttcatggcc gatgtggtgg cggtccagcg gcagatgatc gcgcagctcg
     1321 ccgcggccgg ctgccgctac gtgcatatcg acgcgcccgg ctacaccgcc tacgtcgatc
     1381 cgccgtcgct cgccgcgatg cgcgcgcggg gcgaggaccc ggaggcgacc ttctcgcgct
     1441 cgctcgccgc cgacgccgcc gtcgtcgccg gctttcccgg cgtcaccttc ggcatccatc
     1501 tttgccgcgg caaccagcgc agcatgtggc accgcgaggg cgcgtacgac gccatcgccg
     1561 agcgcctgtt cggcgagctg ccgcatcagc gcttcctcct tgaatacgac agcccgcgcg
     1621 cgggcagctt cgcgccgctg cgcttcgtgc cgcggggccg cacggtcgtg ctgggcctgg
     1681 tcagcagcaa ggtgcccgag ctcgagccgc tcgacgagat ccggcgccgc gtcgacgagg
     1741 cggcgcgcct ggtgccgctc gagcagctcg ccctcagccc gcagtgcggc ttcggctccg
     1801 acgccgtcgg caacctggtc agcgaggacg atcaaaaacg caagctggag cgggtggtcg
     1861 aagccgcccg gacaatctgg gggaaatgat gcaattcgga atcttcgact gggtggaggc
     1921 gaacgatgcg cttgcccccg cggacatcta cgagcacaag ctgcagctcg ccgccgccgc
     1981 cgacaaggcc ggcttccacg gcatgttctt cgccgagcac cagggcacgc cgctttccat
     2041 cgacgcctcg cccgccgtgc tgctcgcggc gctgttccag cgcacgcagc gcctgcgcgc
     2101 cggcgcgctg accttctgcc tgccgtggta cgacccgtac cgcttctaca acgaggtgtg
     2161 catgctcgac cagctctcgg gcgggcggct cgagctgggc gtcgggcgcg gggtgtcgcc
     2221 catcgagtcc ctgatttttg gtttaacttc tatagagcaa agcagagagc ggtaccgcga
     2281 aaccctcgat gcatttttcg ccgcgtgcag ctcgccgctg ctcaatttca agagtcagcg
     2341 tttcgagtac cgcgacgtcg agctcttcaa caagccggtg cagcgcccct accccccgct
     2401 ctggttcccg agctcgaacc gcgactcgat cgagttcacc gcgcgccacg gctaccacac
     2461 cgcgctcatc gcgaagaccg tcgagtcgaa ggcgctgttc aaccagtatc gcgaggtgtg
     2521 ggagaagcac aagggcgacc aggagcggca caacgcccac gtcgcggcgc cgttcctcgc
     2581 caagacccag cacatcgtga tcgccgagac ctacgtcgag gccgagaaga tcggcctgca
     2641 ggcctacgag agctggaagg gccacatgca ccacctgacg cgcaagtacg accgccccga
     2701 ggcgatgaag ctcgagccct tcggcgagga ctcggtgcag cgcctgatcg tcggcccgcc
     2761 gaacaccgtg ttggagaagc tgcaggaagc gatcgacgcg accggcatga actacctgct
     2821 gtgcatcttc tctttcggca acctgccgcc gcaggccgcg ctgcgctccc tcgagatgtt
     2881 cgctgacgag gttatgccca agctcaggga ctgacgcgct tcagcgccct gtcgtagtag
     2941 ctgaggtcca ggtagcgctc gggcccgggc gccttgccgc c
//
LOCUS       DASYJA010000447         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2609996, whole genome shotgun sequence.
ACCESSION   DASYJA010000447 DASYJA010000000
VERSION     DASYJA010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2609996"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VGO02_09780"
     CDS             <1..850
                     /locus_tag="VGO02_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEV7478011.1"
                     /translation="SIEAAEGIATGISAHDRALTVKVASSANARPEDIVQPGHVFPLI
                     AQQGGVLVRAGHTEACCDLARLAGLAPAAVLCEIMRDDGSMARLPDLLEFATEHKLKI
                     GTIADLIEYRSRNESLVQRGAGREITTPWGDFSLVPYRDLAVGSLHLALVLGKIDSKK
                     ETLVRVHEPLSIIDLLDVGKSTHSWGVGEALQAVQQAGNGVVVLMNCSQTGEGLGDAK
                     PPKAGRSMDLRLYGIGAQILRDLGVGRMRLMAAPRKMPSMAGFGLEVVGYLNGPSAGP
                     GAAAKR"
     gene            856..1272
                     /gene="ribH"
                     /locus_tag="VGO02_09785"
     CDS             856..1272
                     /gene="ribH"
                     /locus_tag="VGO02_09785"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEV7478012.1"
                     /translation="MRLGIVASRFNEQIAGELLKRARAEARKLGIEFEEKSVPGALEI
                     PIALQWMAQSGRYDGLVALGAVIRGDTYHFEVVCNESARGVMDVALESGLPVANGILT
                     TEDEAQANARLDKGAEAVRVAVEMCKLRAQIAPGGR"
     gene            1269..>1501
                     /locus_tag="VGO02_09790"
     CDS             1269..>1501
                     /locus_tag="VGO02_09790"
                     /inference="COORDINATES: protein motif:HMM:NF013216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV7478013.1"
                     /translation="MKTAKRQAREIALQALYAWQLSGGDPVEEARSNEDFDKIDAKFV
                     EALLRGVLERSGELEQIITPHLDRQFKKLSPIER"
BASE COUNT          252 a          493 c          540 g          216 t
ORIGIN      
        1 ctcgatcgag gccgccgagg gcatcgccac cggcatttcg gcccatgacc gcgccctgac
       61 cgtaaaagtg gcttcttctg caaatgcaag accggaagat atcgtgcagc ccggacacgt
      121 ctttccgctg atcgcgcagc agggcggcgt gctggtgcgc gccgggcata ccgaggcctg
      181 ctgcgacctg gcgcggctcg ccgggctggc gcccgccgcg gtgctgtgcg agatcatgcg
      241 cgacgacggc agcatggcgc gcctgcccga cctgctcgag ttcgccaccg agcacaagct
      301 caagatcggc accatcgccg acctgatcga ataccgcagc cgcaacgagt cgctggtgca
      361 gcgcggcgcg gggcgcgaga tcacaacgcc atggggagat ttttcccttg tgccgtaccg
      421 tgacctggcg gtcggttccc tccacctcgc gttggttctt gggaagatcg attccaaaaa
      481 agaaacgctg gtgcgagtgc acgagccgct gtcgatcatc gacctgctgg acgtgggcaa
      541 gagcacgcac tcctggggcg tgggcgaggc gctgcaggcg gtgcagcagg cgggcaacgg
      601 cgtcgtggtc ctcatgaatt gcagccagac gggcgaaggg ctgggggacg cgaagccgcc
      661 gaaagccggc cgctcgatgg acctgcgcct gtacggcatc ggcgcgcaga tcctgcgcga
      721 cctgggcgtg gggcgcatgc gcctgatggc ggcgccgcgc aagatgccca gcatggccgg
      781 attcgggctg gaggtcgtgg ggtacctgaa cgggccatcc gccgggcccg gcgccgcggc
      841 gaagcgctga gcgccatgcg cctgggcatc gtcgccagcc gcttcaacga gcaaatcgcc
      901 ggcgagctcc tgaagcgggc ccgcgccgag gcgcgcaagc tgggcatcga gttcgaggag
      961 aagtcggtcc ccggcgccct cgagattccc atcgcgctgc agtggatggc gcagtccggc
     1021 cgctacgacg gcctggtggc gctcggcgcg gtgatccggg gcgataccta ccatttcgaa
     1081 gtggtgtgca acgaatcggc gcgcggcgtg atggacgtcg cgctcgagtc cggcctgccg
     1141 gtcgccaacg gcatcctgac gaccgaggac gaggcgcagg cgaacgcgcg gctggacaag
     1201 ggcgcggaag cggtgcgggt ggccgtcgag atgtgcaagc tgcgggcgca aatcgcgccc
     1261 ggcggccgat gaaaaccgcc aagcgccagg cgcgcgagat cgcgctgcag gcgctctatg
     1321 cctggcagct ttccggcggc gatccggtcg aggaggcccg ctccaacgag gacttcgaca
     1381 agatcgacgc caagttcgtc gaggccctgc tgcgcggcgt gctcgagcgc agcggcgagc
     1441 tcgagcagat catcaccccg cacctcgacc gccagttcaa gaagctttcg cccatcgagc
     1501 g
//
LOCUS       DASYJA010000448         2195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1973591, whole genome shotgun sequence.
ACCESSION   DASYJA010000448 DASYJA010000000
VERSION     DASYJA010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1973591"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1915)
                     /locus_tag="VGO02_09795"
     CDS             complement(83..1915)
                     /locus_tag="VGO02_09795"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7478014.1"
                     /translation="MISATHAPAADAPPPGLLAFYWHFVKQTKGWYAAMFAASLATAL
                     LDILIPVFLGQMVTLMAAADRQAALLAKWPILAGMAAALLIVRPIVVYIDMWVRHSAI
                     LPGVTSLVRWQSHWHVVRQSWPFFQNDFAGRVANRVIQTANALRESVMGVIRGVWYIG
                     IFGTTTFVLLAASDWRLAAPLVVWVMGYVIFLRHYVPRLRELSMKSSEARSLVMARVV
                     DSYTNILTVKLFARMADEDAYVRESVDAHQHAIRGHMEVVTGFVWWMNALNAALFAAI
                     AVIGIWLWAEGAIGGGVVAMALPLAWQITMVGSAVSFEATSIFENVGVVQEGMQSIAV
                     PHGGADRPGAQPLEVSRGEIEFRNVIFAYGRRDAPAVLSDFNLKIQPGERVGVVGRSG
                     AGKTTLVNLLLRFYELESGKILVDGQDIARATQESVRAAVGMVTQDTSLLHRSIAENI
                     RYGRPSASEAQVIEAARKAQADEFIAGLQDWKGNTGYAAHVGERGVKLSGGQRQRIAV
                     ARVVLKDAPVLVLDEATSALDSEVEAAIQEELLDLMQGKTVIAIAHRLSTIARMDRLV
                     VLERGRIVESGTHAELLRLGGLYEALWRRQSGGFIDAGVAQAAE"
     gene            1977..>2195
                     /locus_tag="VGO02_09800"
     CDS             1977..>2195
                     /locus_tag="VGO02_09800"
                     /inference="COORDINATES: protein motif:HMM:NF018180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HEV7478015.1"
                     /translation="MPEPRRYKLNVPESAIVDLRERLERTRFPDEPPGAPWTTGTSVA
                     YLKELLDYWRTRYSWREHEAKLNSFKQYK"
BASE COUNT          353 a          792 c          719 g          331 t
ORIGIN      
        1 gtgcacgaac ctgacggcac accggcgcag atggctcctc cctgccggca cgcggtcgga
       61 accaccttcc ataagtcgca acttactccg ccgcctgcgc cacgcctgcg tcgatgaaac
      121 cgcccgactg gcggcgccag agcgcctcgt acaggccgcc caggcgcagc agctccgcgt
      181 gcgtgccgct ctcgacgatg cggcccctct cgagcacgac cagccggtcc atgcgcgcga
      241 tggtagagag gcggtgcgcg atcgcgatca ccgtcttgcc ctgcatgagg tcgaggagct
      301 cttcctggat ggcggcctcg acctcgctgt cgagcgccga ggtggcctcg tcgagcacca
      361 ggacgggcgc gtctttcagc accacccgag ccaccgcgat gcgctggcgc tggccgccgg
      421 agagcttgac gccgcgctcg ccgacgtgcg ccgcgtagcc ggtgttgccc ttccagtcct
      481 gcagcccggc gatgaactcg tcggcctgcg ccttgcgcgc cgcttcgatc acctgcgcct
      541 cgctcgccga gggccggccg tagcggatgt tctcggcgat ggagcggtgc aggagcgaag
      601 tatcctgcgt caccatgccg accgccgcgc gcacgctctc ctgcgtggcg cgcgcgatgt
      661 cctggccgtc gaccaggatc ttccccgact ccagctcgta gaagcgcagc agcaggttga
      721 cgagcgtcgt cttgcccgcg ccggagcgcc cgaccacgcc gactcgctcg ccgggctgga
      781 tcttcaggtt gaagtcgctc agcaccgcgg gcgcgtcgcg ccggccgtag gcgaagatca
      841 cgttcctgaa ctcgatctcg ccgcgcgaca cctcgagcgg ctgggcgccg ggccggtcgg
      901 cgccgccgtg cggcacggcg atgctctgca tgccctcctg caccacgccg acgttctcga
      961 agatgctggt cgcctcgaag ctcaccgcgc tgcccaccat ggtgatctgc caggcgaggg
     1021 gcagcgccat ggcgaccacg ccgccgccga tcgcgccctc ggcccacagc cagatgccga
     1081 tcaccgcgat ggcggcgaac agcgccgcgt tgagcgcgtt catccaccag acgaagcccg
     1141 tcaccacctc catgtggccg cggatggcgt gctggtgagc gtcgacgctc tcgcgcacgt
     1201 atgcatcctc gtccgccatg cgcgcgaaca gcttgaccgt gaggatgttg gtgtagctgt
     1261 cgaccacgcg cgccatgacc agcgagcgcg cctccgagct cttcatcgac agctcgcgca
     1321 ggcgcggcac gtagtggcgg aggaaaatga cgtagcccat cacccacacg acgaggggcg
     1381 cggccaggcg ccagtcgctc gccgcgagca ggacgaaggt cgtggtgccg aagatgccga
     1441 tgtaccagac gccgcggatg actcccatca cgctctcgcg cagcgcgttg gcggtctgga
     1501 ttacccggtt ggcgacgcgg ccggcgaaat cattctggaa gaagggccag ctctggcgca
     1561 ccacgtgcca gtggctctgc cagcgcacca ggctggtgac gcccggcagg atcgcgctgt
     1621 ggcgcaccca catgtcgatg tacacgacga tcgggcgcac gatcagcagc gccgccgcca
     1681 tgcctgcgag gatcggccac ttggcgagca gcgcggcctg gcggtcggcg gcggccatca
     1741 gcgtcaccat ctggccgagg aacaccggta tcaggatgtc gagcagcgcc gtggcgaggc
     1801 tcgccgcgaa catcgccgcg taccagccct tcgtctgctt gacgaagtgc cagtagaacg
     1861 cgagcagccc gggcggcggt gcgtccgcgg cgggcgcatg cgtcgcgcta atcacatggg
     1921 gtttcaacgg gttctgtctt tccacttcca aagcggcgcc acgataacat ggccgcatgc
     1981 ccgagccgcg ccgctacaag ctcaacgttc ccgaatccgc gatcgttgac ctgcgcgagc
     2041 gcctcgagcg cacgcgcttc cccgacgagc cgcccggcgc gccctggacg acgggtacca
     2101 gcgtcgccta tttgaaagaa ttactcgact actggcgcac ccggtactcg tggcgcgagc
     2161 acgaggccaa gctgaacagc ttcaagcagt acaag
//
LOCUS       DASYJA010000449         6461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2517822, whole genome shotgun sequence.
ACCESSION   DASYJA010000449 DASYJA010000000
VERSION     DASYJA010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2517822"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1625)
                     /locus_tag="VGO02_09805"
     CDS             complement(<1..1625)
                     /locus_tag="VGO02_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEV7478016.1"
                     /translation="MKIEDYALIGDCHSAALVGRDGSIDWLCWPRFDSPACFAALLGT
                     PDNGFWKIAPVAPEARSTRRYRPDSLILETEFETAEGAVTLIDFMPVRDGHADLVRIV
                     TGKRGRVAMESVGALRFDYGAALPWITLDEGGHRLRAIAGPDLIELRSPVPLESRERR
                     TCARFAVGEGESVAFVMTHAPSHLAVRGALDPKAALAETERFWTKWAARCTVQGEFAG
                     QARRSLITLKALTYAPTGGIVAAPTASLPEKIGGVRNWDYRFCWLRDATLSLLAFMNG
                     GYYEEARAWREWLVRAVAGSPAQMQIMYGIAGERRLTEWQLDWLAGYEGSKPVRCGNA
                     AAEQLQLDVYGEVMDALYQACKGGVARHDQAWSLQRALLEHLETAWIQPDHGIWEVRG
                     APRHFTHSKVMAWVAFDRGVKMAEHFGMQGPVARWREVRARIHDEVCSRGVSTKRGCF
                     VQSYGSEELDASLLLIAQTGFLPARDPRVAATIDAVQRELTVDGLVLRYLTHESLDGL
                     PPGEGAFLACSFWLADALCMLGRRDEAHALFKRL"
     gene            1772..3067
                     /locus_tag="VGO02_09810"
     CDS             1772..3067
                     /locus_tag="VGO02_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEV7478017.1"
                     /translation="MILLWVLPALGVLAYILLGVNRVQRRAARLRRGTPRHRSGLSQP
                     AGEPGTHFAPLARLVGEIVGRPLLPGNSVEPLLNGVEAYPAMLEAIGGAQRSIMLGSY
                     LFDDDGVGAQFVEALVAAKARGVEIRVLIDDVSNRFSRHAASKPLRRAGIEVGIFNPP
                     FLPARLHAANLRNHRKIMVVDGALGFTGGMNIASRYWREGPDQAFRDLHFRLRGPVVT
                     HLAEVFCDDWAFAVAERLQGETWFPPLAPAGSVLARGIESGPDEAFERVRWAMIGALN
                     AAQRSVRVLTPYFIPDGTLISCLNAAALRGVDVDIVLPEETDLPHVRWAAFGQLWQVL
                     DHGCEVYLRPGPFDHSKLLVVDGAYVLLGSANWDARSLRLNFEFDVECYSVELGAKLD
                     GLVEARISQSRPYTMASSNARPLPVKLRDGIARLFAPYL"
     gene            complement(3078..4361)
                     /locus_tag="VGO02_09815"
     CDS             complement(3078..4361)
                     /locus_tag="VGO02_09815"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEV7478018.1"
                     /translation="MKSLAALLTVALLLTPARSAARCAPDNGGLTLPAGFCASVFADN
                     LGQARHLAVAVDGTVYVNTWRSPYREGAKVAPGGFLVALRDADGDGVAELVERFGEDS
                     SGRATGGTGIAIHKGQLYAEESGRIVRYALVLGKLVPRGERDIVVDNLPTKGGHTMHP
                     FVISRDGALFINVGSASNACQVKDRAPHSPGRQPCEELDERAGLWRYDASLPAQLFER
                     GGRYATGLRNAVALAMHPSAGLVVLQHGRDQLHENWPEKFSLQQGAELPAEAMFRIKY
                     GADYGWPYCYYDGREDRHVLAPEYGGDGRETGRCRGLPAPVAAYPAHWAPNALLFYTG
                     SAFPERYRGGAFIAFHGSWNRPQQQGYNLVFQPLDRDGAPSGGFEVFADGFAGPVRTP
                     EGARHRPSGVAMGPDGALYVSDDQRGRVWRIVPGK"
     gene            4472..5236
                     /locus_tag="VGO02_09820"
     CDS             4472..5236
                     /locus_tag="VGO02_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEV7478019.1"
                     /translation="MKSHKRTTRKKFSAAEMQKRVDLAAAYRLVHLFGMDEMIANHIS
                     ARVPGKDHVFYINPYGMLYEQMHASCFVELDLDGNVVYNPTEYGINQAGFVLHSAVHK
                     ARHDVDCVIHTHTLAGMAVSAMKCGLLPLAQTAMRFTDVAYHDYEGVALNLDEQARVV
                     RDLGTREAMILRNHGLLVVGASIAEAFNNIFRLERACQLQVTALSCNTELAMPPKDLI
                     EATNKLYLPGVRRRFGLLEWPALLKKLDQLDPSYRK"
     gene            5243..5635
                     /locus_tag="VGO02_09825"
     CDS             5243..5635
                     /locus_tag="VGO02_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEV7478020.1"
                     /translation="MNADERRIRDLIAQWHRATAAGNVDAILRLMSEDVVFLVPGKPP
                     MQGRDQFEKGLRGLLKSHRIESSGKIEEVRVAGDLAYSWTTLTVRITPLSGGDGMERS
                     GSALSVFRKQANGTSWLLVRDANLLPPA"
     gene            5735..>6461
                     /locus_tag="VGO02_09830"
     CDS             5735..>6461
                     /locus_tag="VGO02_09830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEV7478021.1"
                     /translation="MCILCDEGKPQDHARSRTQLGRRDFLKASTATAAAAAGMSLFNA
                     PPAAAHDGDGPPRDSGKQGRRYVIRNGHVMSMDPTVGDIVRGDVLVEGKKIKAIGRNL
                     QAGGADEIDAEGRIVMPGFIDTHHHQFETALRSFLANGILINDGSGSPSSNPTYFEFI
                     LLKFAPLYRPQDVYISELFGGLAQLDNGVTTVHDVSQIHHSPQHSDAAVQALFDTGRR
                     AAFGFFESAGANILGTNPGNKYPT"
BASE COUNT         1006 a         2342 c         2161 g          952 t
ORIGIN      
        1 aggcgcttga aaagcgcgtg cgcctcgtcg cgccgcccga gcatgcacag cgcgtcggcg
       61 agccagaagc tgcaggcgag gaacgcgccc tcgcccgggg gcaggccgtc gagcgactcg
      121 tgcgtcaggt agcgcagcac caggccgtcg acggtgagct ctcgctgcac agcgtcgatg
      181 gtggcggcca ccctcgggtc cctggcggga aggaaaccgg tctgcgcaat gagcagcagg
      241 ctcgcgtcga gctcctccga gccgtagctc tgcacgaagc agccgcgctt cgtgctcaca
      301 ccgcggctgc agacctcgtc gtggatgcgc gcgcggacct cgcgccagcg cgcgaccggc
      361 ccctgcatgc cgaagtgctc ggccatcttc acgccgcggt cgaaggcgac ccacgccatg
      421 accttggaat gggtgaagtg gcgcggcgcg ccgcgcactt cccagatgcc gtggtcgggc
      481 tggatccacg ccgtctccag gtgctcgagc agcgcgcgct gcagcgacca ggcctggtcg
      541 tgccgcgcca cgccgccctt gcacgcctgg tagagcgcat ccatcacctc gccgtagacg
      601 tcgagctgca gctgctcggc ggcggcgttg ccgcagcgca ccggcttcga gccttcgtag
      661 ccggcgagcc agtcgagctg ccactcagtc aggcggcgct cgccggcgat gccgtacatg
      721 atctgcatct gcgccgggct gccggccacc gcgcgcacca gccactcgcg ccaggcgcgc
      781 gcttcctcgt agtagccgcc gttcatgaag gcgagcagcg agagcgtggc gtcgcgcagc
      841 cagcagaagc ggtagtccca gttgcgcacg ccgccgatct tctccggcag cgaggcagtg
      901 ggcgcggcga cgataccgcc ggtgggcgcg taggtaagcg ccttcagcgt gatcagcgag
      961 cggcgtgcct ggcccgcgaa ctcgccctgc accgtgcagc gcgcggccca cttagtccag
     1021 aatcgctcgg tctccgcaag cgcggccttc ggatcgagcg cgccgcgcac ggcaaggtgc
     1081 gacggcgcgt gcgtcatgac gaaggcgacg ctttctccct cgcccaccgc gaagcgcgcg
     1141 caggtgcgcc gctcgcggct ctcgagcggc accggcgagc gcagctcgat caggtctggg
     1201 ccggcgatcg cgcgcagccg atgcccgccc tcgtcgagcg tgatccaggg cagcgcggcg
     1261 ccgtagtcga agcgcagcgc gcccaccgac tccatggcca cgcggccgcg cttgccggtc
     1321 acgatgcgca ccaggtcggc gtgcccatcg cgcaccggca tgaaatcgat cagggtgacc
     1381 gcgccctcgg cggtctcgaa ctcggtctcg aggatcaagc tgtcggggcg gtagcggcgc
     1441 gtcgagcgcg cctcgggcgc gaccggggcg atcttccaga agccgttgtc cggcgtgccg
     1501 agcaacgcgg caaagcaggc gggcgaatcg aagcgcggcc agcacagcca gtcgatcgag
     1561 ccgtcgcggc cgaccagcgc cgcactgtgg cagtcgccga tcagcgcata gtcctctatc
     1621 ttcatgagaa actgaccgcg ccgcgacggg aatagttcat ctgaccgaca ccctctacct
     1681 cgccttcacc ctcggcgccg ccggcgcggc gctggtcgct tcggcgcatg ccgtcatcta
     1741 caagcgcgaa tcacgctcgg cgtcgatctg ggtgatcctg ctgtgggtgc tgccggcgct
     1801 cggcgtgctg gcctacatcc tgctcggcgt caatcgggtg cagcgccgcg ccgcgcggct
     1861 gcgccgcggc acgccgcgcc accgcagcgg cctgtcgcag cctgccggcg agccaggcac
     1921 gcacttcgcg ccgctcgcgc gcctggtcgg cgagatcgtc ggccggccgc tgctgccggg
     1981 caattcagtc gagccgctgc tgaacggcgt cgaggcctac ccggccatgc tcgaggcgat
     2041 cggcggcgcg cagcgctcga tcatgctcgg ctcctacctc ttcgatgacg acggcgtcgg
     2101 cgcgcagttc gtcgaggcgc tggtcgcggc gaaggcgcgc ggcgtggaga tccgcgtgct
     2161 gatcgacgac gtctccaacc gcttctcgcg ccacgcggcg tccaagccgc tgcggcgcgc
     2221 cggtatcgag gtcggcatct tcaacccgcc gttcctcccg gcgcggctgc acgccgccaa
     2281 tctgcgcaac caccgcaaga tcatggtggt cgacggcgcg ctggggttca ccggcggcat
     2341 gaacatcgcg agccgctact ggcgcgaagg ccccgaccag gcgttccgcg acctgcactt
     2401 ccgcctgcgc gggccggtgg tgacgcacct cgccgaggtg ttctgcgacg actgggcgtt
     2461 tgccgttgcc gagcggctgc agggcgagac gtggtttccg ccgctcgcgc cggcgggcag
     2521 cgtgctcgcg cgcggcatcg aatcgggccc cgacgaggcc ttcgagcgcg tgcgctgggc
     2581 gatgatcggc gcgctcaacg cggcgcagcg ctcggtgcgc gtcctcacgc cctacttcat
     2641 ccccgacggc acgctcatct cctgcctgaa cgccgccgcg ctgcgcggcg tcgacgtcga
     2701 catcgtgctg ccggaggaga ccgacctgcc gcacgtgcgc tgggccgcct tcggccagct
     2761 ctggcaggtc ctcgaccacg gctgcgaggt gtacctgcga cccgggccgt tcgaccacag
     2821 caagctgctg gtggtcgacg gcgcctacgt gctgctcggc tcggcaaact gggacgcgcg
     2881 cagcctgcgc ctcaacttcg agttcgacgt cgagtgctac agcgtcgagc tcggcgccaa
     2941 gctggacggc ctggtcgagg cgcgcatttc gcaatcacgc ccttacacca tggcgagctc
     3001 gaacgcccgg ccgctgcctg tcaagctgcg ggacggcatc gcacggctgt tcgctcccta
     3061 cctgtgacag ctccgcgtca tttgcccggc acgatgcgcc acacacggcc gcgctgatcg
     3121 tcgctgacat acagcgcgcc gtcgggtccc atcgccacgc ccgagggccg gtgcctggcg
     3181 ccttcgggcg tcctgaccgg gccggcgaag ccgtcggcga atacctcgaa gccgccgctc
     3241 ggcgcgccgt cgcggtccag cggctggaac accaggttgt agccttgctg ctgcggccgg
     3301 ttccacgagc cgtggaaggc gatgaacgcg ccgccgcgat agcgctccgg gaacgcgctg
     3361 cccgtataga agagcagcgc attcggcgcc cagtgcgccg gatacgccgc caccggagcc
     3421 ggcaggccgc ggcagcggcc ggtctcgcgc ccatcgccgc cgtactcggg cgcaagcaca
     3481 tgcctgtctt cgcggccgtc gtagtagcag taaggccagc cgtagtcggc gccgtacttg
     3541 attcggaaca tcgcttcggc cggcagctcg gcgccctgct gcaggctgaa tttctccggc
     3601 cagttctcgt gcagctggtc tcggccgtgc tgcagcacga ccaggcctgc ggacgggtgc
     3661 atggcgaggg ccaccgcgtt gcgcagcccc gtcgcatagc ggccgccgcg ctcgaagagc
     3721 tgcgcgggca ggctggcgtc gtaccgccag aggccagcgc gctcgtcgag ctcctcgcag
     3781 ggctgccggc ccggggagtg gggggcccgg tccttcacct ggcaggcatt cgacgccgag
     3841 ccgacgttga tgaacagggc accgtcacga ctgatcacga acgggtgcat ggtgtgcccg
     3901 ccctttgtcg gcaggttgtc gacgacgatg tcgcgctcgc cgcgcggcac cagcttccct
     3961 agcaccagcg cgtagcgcac gatgcggccg ctttcctccg cgtagagctg gcccttgtgg
     4021 atagcgatgc cggtgccgcc ggtcgccctg cccgagctgt cttcgccgaa acgctcgacc
     4081 agctcggcga cgccgtcgcc gtccgcgtcg cgcagcgcca ccaggaaacc gccgggcgcg
     4141 actttcgcgc cctccctgta gggcgagcgc caggtattca cgtagacggt gccatcgacg
     4201 gctaccgcca ggtgccgggc ctggcccagg ttgtcggcaa aaacgctcgc gcagaagccg
     4261 gcgggcagcg tcaggccgcc gttatccggc gcgcaccgcg ccgcggaccg ggcgggggtg
     4321 agaagcagcg cgaccgtcag cagcgctgcc agcgatttca tgcgagaacg ctacaccgcc
     4381 acccgccggc gctgttcctg atccgtgcgc ggccatgcgc gcttttttgc taagctgcgg
     4441 aacggcatcg cacgggctat ccgtccccac catgaaatcg cacaagcgaa ccacccgcaa
     4501 gaagttcagc gctgcggaga tgcagaagcg ggtcgacctc gcggccgcgt atcgcctggt
     4561 ccatctcttc ggcatggacg agatgatcgc caaccacatc tcggcacggg tgccgggcaa
     4621 ggaccacgtc ttctacatca acccgtacgg catgctctac gagcagatgc acgcctcctg
     4681 cttcgtcgag ctcgacctcg acggcaacgt ggtctacaac ccgaccgagt acggcatcaa
     4741 ccaggcgggc ttcgtgctgc acagcgccgt gcacaaggcg cgccacgacg tcgactgcgt
     4801 gatccacacc cacacgctgg ccggcatggc ggtttcggcc atgaaatgcg gcctgctgcc
     4861 gctcgcgcag accgcgatgc gcttcaccga cgtcgcctac cacgattacg agggcgtggc
     4921 cctcaacctc gacgagcagg cccgcgtggt gcgcgacctg ggaacgcgcg aggcgatgat
     4981 cctgcgcaac cacggcctgc tggtggtcgg cgcgagcatc gccgaggcct tcaacaacat
     5041 cttccgcctc gagcgcgcct gccagctgca ggtgacggcg ctgtcctgca acaccgagct
     5101 cgccatgccg cccaaggacc tgatcgaggc gaccaacaag ctctacctgc ccggcgtgcg
     5161 ccgccgcttc ggcctgctcg aatggccggc gctgctgaag aagctcgacc agctcgatcc
     5221 gtcgtaccgg aaatagatcg ccttgaacgc agacgagcgc aggatccgcg acctgatcgc
     5281 gcagtggcat cgcgcgacgg cggcgggaaa tgtggatgcc atcctgcgcc tcatgtccga
     5341 ggacgtggtc ttcctggtgc ccggcaagcc gccgatgcag ggccgcgacc agttcgagaa
     5401 ggggctgcgc ggactgctca agtcgcaccg cattgagtcg agcggcaaga tcgaggaagt
     5461 ccgcgtggcg ggggatctcg cctacagctg gaccaccctc accgtgcgca tcacgccgct
     5521 gtccggcggc gacggcatgg agcgctcggg cagcgcgctg tcagtcttcc gcaagcaggc
     5581 gaacggcacc agctggctcc tggtgcgcga cgccaacctt ctgccaccgg cctagggctc
     5641 aaaaggtggg cgtgatccca cgcccctcac aagaaaagat ttactccggc gcggcgcggg
     5701 accagaatgc actcacccag aggagggtca acacatgtgc atactttgcg atgaaggcaa
     5761 gccgcaggac cacgctcgca gccgcacgca gctcggacgg cgcgatttcc tgaaggcctc
     5821 gaccgcgacc gccgccgcgg ccgcgggcat gagcctgttc aacgcgccgc cggccgcggc
     5881 gcatgacggc gacggcccgc cgcgcgacag cggcaagcag ggccggcgct acgtgatccg
     5941 caacggccac gtcatgtcca tggaccccac cgtcggcgac atcgtgcgcg gcgacgtgct
     6001 ggtagaaggt aagaagatca aggccatcgg gcgcaacctg caggccggcg gcgccgacga
     6061 gatcgacgcc gagggccgca tcgtgatgcc cggcttcatc gacacgcacc accaccagtt
     6121 cgaaaccgcc ctgcgcagct tcctcgccaa cggcatcctg atcaacgacg gctcgggctc
     6181 gccgagctcg aatcccacgt atttcgaatt cattctgctc aagttcgcgc ccttgtaccg
     6241 cccgcaggac gtctacatca gcgagctgtt cggcgggctg gcgcagctcg acaacggcgt
     6301 gacgacggtg cacgacgtct cgcagatcca ccattcacca cagcactcgg atgccgccgt
     6361 gcaggcgctg ttcgacaccg gtcgccgcgc cgccttcggc ttcttcgaga gcgcgggcgc
     6421 caacatcctc ggcaccaacc ccggcaacaa gtacccgacc g
//
LOCUS       DASYJA010000450         4736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3246069, whole genome shotgun sequence.
ACCESSION   DASYJA010000450 DASYJA010000000
VERSION     DASYJA010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3246069"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VGO02_09835"
     CDS             complement(<1..290)
                     /locus_tag="VGO02_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEV7478022.1"
                     /translation="MNIREKAEKFVALHRGPSAFVIANPWDAGSARILAGLGYQALAT
                     SSGASAGVLGKRDGRVTRDEALAHARLIVEAVDLPVSADLEKGFGDRPEEAA"
     gene            complement(287..874)
                     /locus_tag="VGO02_09840"
     CDS             complement(287..874)
                     /locus_tag="VGO02_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEV7478023.1"
                     /translation="MGKARMEAFSDGVIAVIITVMVLELKVPHNEDIAALAPLFPVFL
                     TYVLSFVYVGIYWNNHHHMLHACQRVTGSVLWANLHLLFWLSLVPFTTGWMGENHFAA
                     VPTALYGAVLLMAAIAYWILQQLIIAAQGPDSLLKKAVGGDWKGKLSPFVYVLAILAA
                     FKAQWISQALYVAVALIWLVPDRRIENALRAREGS"
     gene            complement(874..1140)
                     /locus_tag="VGO02_09845"
     CDS             complement(874..1140)
                     /locus_tag="VGO02_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478024.1"
                     /translation="MRTAIIIAVGFLLWGLCLGIAKLTSNLTAPSATVATAAFVVLWF
                     IAAAVNMWVGVAKAGYSFMEELPIFLVIFLVPCAVAVAVKWRFL"
     gene            complement(1142..2191)
                     /locus_tag="VGO02_09850"
     CDS             complement(1142..2191)
                     /locus_tag="VGO02_09850"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEV7478025.1"
                     /translation="MAPTAALRRLLGTELPVIQAPMAGVQVSALTVAVSNAGGLGSLP
                     CAMLTLEVMRKELAAIKAATGKPYNVNFFCHSPPVASTQREAAWRSALSPYYREYGID
                     AGSIPAGPGRAPFSAEAADVLEEFKPRVVSFHFGLPPADLLARVRSWGSKILSSATTV
                     NEARWLEAHGVDAIIAQGSEAGGHRGIFLSDDLSTQVGTFALVPQVVRAVKVPVIAAG
                     GIADADGVAAALALGAAGVQVGTAYLLCPEATTSPLHRAALKSEAARETALTNLFTGR
                     PARGIVNRIIKELGAINPAAPAFPLATSAIAPLRAKAEAQGKGDFSPLWSGQNASGCK
                     EIPAADLTRELAARL"
     gene            complement(2200..3264)
                     /locus_tag="VGO02_09855"
     CDS             complement(2200..3264)
                     /locus_tag="VGO02_09855"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEV7478026.1"
                     /translation="MNARVAAVLLVLLVVLGGTALIYQREDRQRRPSNAASLGQVLFK
                     DLKAADIASIRIAEAKNVLTLERKDERWTLAERGGFPADFARVREFVLKAIELKAGQS
                     EPIGEKDRARLNLDDSGTQIDFRGAGGKPLGTLIIGKKYFKREVENPAKAIGDGRFVL
                     QPADPKIVYLVADPLIQATAKTGEWIERAAFQAEKVKTLEVKFPEGGGYRIERNADNA
                     DWKLAGSKPGEKLDISKANAASYSLSLLDLADVLPNDTKDTGLDKPAEITAVTLDGLT
                     YSIKVGKLQGDNYFTTFTSSGTIKPDAKDAERSKKLEERQPRERMLQNYVLLIPKGRL
                     EDTLKKRPEFLEKREDKKKK"
     gene            complement(3261..>4736)
                     /locus_tag="VGO02_09860"
     CDS             complement(3261..>4736)
                     /locus_tag="VGO02_09860"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEV7478027.1"
                     /translation="QLVSGEQFYLGVAVSQLERKQAIPAISPQRERLIEYDLVRAVAR
                     VGSAERPKIGLMAGLPVLGEKFNPFTRQSSEPWVLASELKREFEVKEVPISAKEIDKD
                     LNVLLLIHPRLGIPPTLEYALDQFVLRGGKLIAFVDPYAYFDQQQPMMPGMPPEPSSS
                     TLPALFKAWGVEMDPGKVIADVVFGSGAGARYTPTVLSLNRTALSRDDVVTSSVETLL
                     YAFGGAFQIKNTEGIRVTELIKSSPNSMLVDNMNATKSGDEATRNFKPSGQAMPLAVR
                     LTGKFKTAFPEGLQVDKKPVAGTPALKESTAENSVILVADVDLLADGAAVDVQDVFGR
                     RVVVPSNGNLALAMGMVEQLAAGDDLISLRSRTASFRPLTVVRELEAEAQKQYFGRIQ
                     ALEDELQKTTAKLQELQKAQGAPGAKGQQILSAEQQAELERFRKTVAEGRLALKEVRK
                     NLRQDAEALVFWTKLANIALMPLLVALFGLGFALIRRTRLA"
BASE COUNT          750 a         1574 c         1590 g          822 t
ORIGIN      
        1 tccgccgctt cctcgggccg gtcgccgaaa cccttctcca gatcggccga aaccggcaag
       61 tcgaccgcct cgacgatcag gcgcgcgtgc gccagcgcct cgtcgcgcgt cacgcgcccg
      121 tcgcgcttgc cgagcacgcc ggccgaggcg ccgcttgaag tcgccagcgc ctgatacccc
      181 agccctgcca ggatgcgcgc cgagcccgcg tcccagggat tggcgatgac gaacgcgctt
      241 ggcccgcggt gcagggcgac gaacttctcg gccttctctc tgatgttcat gatccctccc
      301 tggccctgag cgcattctcg atgcgcctgt cgggcaccag ccagatgagt gcgacggcga
      361 cgtaaagagc ctgcgagatc cactgcgcct tgaaagccgc gaggatggcg agcacgtaga
      421 cgaacggcga cagctttccc ttccagtcgc ctcccaccgc cttcttcagc agcgagtccg
      481 gcccctgcgc ggcgatgatg agctgctgca ggatccagta ggcgatggcc gccatgagca
      541 gcaccgcgcc gtaaagcgcg gtcggcaccg cggcgaagtg gttctcgccc atccagccgg
      601 tggtgaaggg aaccagcgat agccagaaga ggaggtggag attcgcccag agcaccgatc
      661 ccgtcacccg ctggcaggca tgcagcatgt gatggtggtt gttccaatag atacccacgt
      721 acacgaagct cagaacgtaa gtcaggaaca cagggaacag cggcgcaagc gccgcgatgt
      781 cctcgttgtg cggcaccttc agctccagca ccatgacggt gatgatcacc gcgatcacgc
      841 cgtcgctgaa cgcctccatc cgcgccttgc ccatcacagg aaccgccact tgaccgccac
      901 ggcgaccgcg cagggcacca ggaagataac cagaaagatc ggcagctcct ccatgaacga
      961 gtagccggcc ttcgctacac cgacccacat gttgaccgcc gctgcgatga accagagaac
     1021 gacaaaagcc gcagtggcga cggtcgcaga tggggcagtg aggttcgagg tcagctttgc
     1081 gatgccgagg cagagccccc atagcagaaa accgaccgcg atgatgatgg cggtccgcat
     1141 gctagagcct cgccgccagc tcgcgagtca ggtcggcggc cgggatttcc ttgcagccgc
     1201 tcgcgttctg gccggaccat agcggcgaga agtcgccctt gccctgcgcc tccgcctttg
     1261 cccgcaatgg ggcgatcgcc gaggtggcga gcgggaacgc aggcgccgcg gggttgatgg
     1321 cgcccagctc cttgatgatg cggttgacga tgccgcgcgc ggggcggccg gtgaagaggt
     1381 tcgtgagagc agtttcgcgc gccgcttcgc ttttaagcgc cgcgcggtgc aaagggctgg
     1441 tggtggcttc ggggcaaagc agataggcag tgcccacctg cacgccggcc gcgccgagcg
     1501 cgagcgcggc cgcgacgcca tcggcgtcgg cgataccgcc ggcagcaatc accggtacct
     1561 ttaccgcgcg gacgacttgc gggaccaggg caaacgttcc gacctgcgta ctcaggtcgt
     1621 ccgacaagaa gatgccgcgg tggccgccgg cctcgctgcc ctgggcgatg atggcatcca
     1681 cgccgtgtgc ttcgagccag cgcgcctcgt tgacggtggt cgccgaggaa aggatcttcg
     1741 agccccagga gcgcacgcgc gccagcaggt ccgccggcgg caggccgaaa tggaagctca
     1801 ccacccgcgg cttaaattcc tcgagcacat cggcggcttc ggcgctgaac ggcgcgcgcc
     1861 ccggcccggc cgggatgctg ccggcgtcga tgccgtattc cctgtaatag ggcgagagcg
     1921 ccgaacgcca ggccgcttcg cgctgcgtgc tcgccacggg cggcgagtgg cagaagaaat
     1981 tgacgttgta gggcttgcct gtggcggcct tgatcgccgc cagctccttg cgcatgacct
     2041 cgagggtgag catggcgcag ggcaatgagc ccaggccgcc ggcattggac accgctaccg
     2101 tgagagcgct gacctgcact ccggccatgg gcgcctggat gacggggagc tcggtgccca
     2161 gaagccgccg taacgccgcg gtgggagcca tgcccaatgc tacttctttt tcttgtcttc
     2221 cctcttctcg aggaattccg ggcgcttctt gagggtgtcc tcgaggcggc ccttgggaat
     2281 cagcaggaca taattctgca gcatcctttc gcgcggctgg cgctcttcga gcttcttgct
     2341 gcgctcggcg tccttggcat cgggcttgat ggtgcccgac gaggtgaagg tggtgaagta
     2401 gttgtcgccc tgcagcttgc cgaccttgat gctgtaggtg aggccatcga gggtcacggc
     2461 ggtgatctcg gccggcttgt ccaagcccgt gtcttttgtg tcgttcggca ggacgtcggc
     2521 gaggtcgagc aggctgaggg aatacgaagc ggcattcgcc ttgctgatgt cgagcttctc
     2581 gccgggcttc gagccggcga gcttccagtc ggcgttgtcg gcgttgcgct cgatgcggta
     2641 gccgccgccc tcggggaatt tcacctcgag ggtcttcacc ttctccgcct gaaaggcggc
     2701 gcgctcgatc cactcgcccg tcttggcggt ggcctggatc agcgggtcgg cgaccaggta
     2761 gacgatcttc gggtcggcgg gctgcaggac gaagcggccg tcgccgatcg cctttgccgg
     2821 gttctccacc tcgcgcttga agtacttctt gccgatgatg agcgtgccga gcggcttgcc
     2881 gccggcgccg cggaaatcga tctgcgtgcc gctgtcgtcg aggttgaggc gggcgcgatc
     2941 cttctcgccg atcggctcgc tctggccggc cttgagctcg atcgccttca gcacgaactc
     3001 gcgcacccgg gcgaaatcgg ccgggaagcc gccgcgctcg gcgagggtcc agcgctcgtc
     3061 cttgcgctcg agcgtcagca cgttctttgc ctcggcgatg cggatcgagg cgatgtcggc
     3121 cgccttgagg tccttgaaga gcacctggcc gaggctggcg gcgttcgacg gccggcgctg
     3181 cctgtcctcg cgctggtaga tgagcgccgt gccgccgagc acgacgagca gcaccagcag
     3241 gacggcggcg acgcgggcgt tcaagcgagc ctggttcttc tgatcaaggc aaagcccagg
     3301 ccgaagagcg cgaccagcag cggcatcagc gcgatgttcg ccagcttggt ccagaagacc
     3361 agcgcttcgg cgtcctggcg caggttcttg cgcacttcct tcagcgcgag gcggccttcg
     3421 gcgacggtct tgcgaaagcg ctcgagctcg gcctgctgct cggcgctcag gatctgctgg
     3481 cccttggcgc cgggcgcgcc ttgcgccttt tgcagctcct gcagcttggc ggtggtcttc
     3541 tgcagctcgt cctcgagcgc ctggatcctg ccgaagtact gcttctgcgc ctcggcctcg
     3601 agctcgcgca ccacggtcag cggtctaaat gaagcggtgc ggctgcgcag cgagatcagg
     3661 tcgtcgcctg cggcaagctg ctcgaccatg cccatggcga gcgccaggtt gccgttggag
     3721 ggcaccacca cgcggcgccc gaacacatcc tgcacgtcga ccgcggcgcc gtcggcgagc
     3781 agatcgacgt cggcgaccag gatcaccgag ttctcggccg tcgattcctt cagcgccggc
     3841 gtgcccgcca ccggcttctt gtcgacctgc aggccctcgg ggaacgcggt cttgaacttg
     3901 cccgtaaggc gcaccgcgag cggcatcgcc tgcccggagg gcttgaagtt gcgcgtcgcc
     3961 tcgtcgcccg acttggtggc gttcatgttg tccaccagca tcgagttggg cgagctcttg
     4021 atcagctcgg tgacccggat tccctcggta ttttttattt gaaacgcacc accaaaggcg
     4081 taaagcagcg tttcaacact gctggtgacc acgtcgtcgc ggctgagcgc ggtgcggttg
     4141 agcgagagaa cggtgggcgt gtagcgcgcg ccggcgcccg agccgaacac cacgtcggcg
     4201 atcaccttgc cggggtccat ctccacgccc caggccttga acagggccgg cagcgtcgag
     4261 ctcgacggct ccggcggcat gccgggcatc atcggctgct gctggtcgaa gtaggcgtag
     4321 ggatcgacga aggcgatcag cttgccgccg cgcagcacga actggtcgag cgcgtactcg
     4381 agggtcggcg ggatgcccag gcgggggtgg atcagcagca ggacgttgag gtccttgtcg
     4441 atctccttcg cgctgatcgg cacttccttc acttcgaact cgcgcttcag ctcgctcgcc
     4501 agcacccacg gctccgagct ctggcgcgtg aacgggttga acttctcgcc cagcaccggc
     4561 aggccggcca tcaggccgat cttcggccgt tcggccgagc cgacgcgcgc gacggcgcgc
     4621 accaggtcgt actcgatcag ccgctcgcgc tgcggcgaga tggcggggat cgcctgcttg
     4681 cgctcgagct ggctcaccgc cacgccgagg tagaactgct cgccgctcac cagctg
//
LOCUS       DASYJA010000451         2234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_522693, whole genome shotgun sequence.
ACCESSION   DASYJA010000451 DASYJA010000000
VERSION     DASYJA010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522693"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..1633
                     /locus_tag="VGO02_09865"
     CDS             227..1633
                     /locus_tag="VGO02_09865"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HEV7478028.1"
                     /translation="MPERWTRDSWRKKPVLQVPDYPDQAALEAVERQLAGFPPLVFAG
                     EARSLKKALGRVAMGEGFLLQGGDCAESFSEPSANNFSANNIRDFLRVFLQMSVVLTY
                     AAALPVVKVGRIAGQFAKPRTSPMEKKDGKELPSYRGDVINGVEFTDEARRPDPARQI
                     EAFRHSAATLNLLRAFVQGGYANLANVRQWTLGFVKDSPQSHHYEELADRISEALGFM
                     QACGLDLESHPELKNTEFYTSHEALLLGYEQAMTREDSTHPGSFCCTSAHMLWIGDRT
                     RQPDHGHVEFCRGIINPLGLKCGPSSKIDEMLRLIDILNPENEPGRLTLIIRLGSDKV
                     GDQLPAMIRAVQREGKVVLWSCDPMHGNTITSETGYKTRPFERILSEVKTFFAVHRAE
                     GTHGGGVHLEMTGQNVAECTGGARMITDADFKDRYHTVCDPRLNAEQAIDLAFLLADL
                     LKAERTAKARPLPAAAGL"
     gene            1811..2164
                     /locus_tag="VGO02_09870"
     CDS             1811..2164
                     /locus_tag="VGO02_09870"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEV7478029.1"
                     /translation="MERLLRATINTWHGLLAAARSEEAFRQELVALVLAVPLAFLLAD
                     EGWKRLALIGVIVFVLVVELLNTAIEKLADRLTRDHDPQIRDVKDMGSAAVGVGLAIA
                     GLTWLVAIAERLGFV"
BASE COUNT          386 a          740 c          733 g          375 t
ORIGIN      
        1 cgcttcttct cggcaagccg tgcgacaagc cgggacgggc gcgccgggca gtccggacgt
       61 caaagtgccg atggaacaaa gagttagagc aatcaccggc ggcgcgccgc agccgcccca
      121 gcgggtggtc gggggacgga agtcctggct tgcggcgcct gccgtcgtgt ataagcccgt
      181 ggaaaatctc gcccgtactt gggcttaagt cgagtggagg ggcgtcatgc ccgagcgttg
      241 gacgcgcgac agttggcgca agaagcccgt actccaggtc cccgactacc ccgaccaggc
      301 ggcgctcgaa gcggtcgagc ggcagctcgc cggctttccg ccgctggttt ttgccggtga
      361 ggcgcgcagc ctgaagaagg cgctcggccg ggtcgccatg ggcgaaggtt tcctgctgca
      421 agggggcgac tgcgccgaaa gcttctccga gcccagcgcc aacaatttca gcgccaacaa
      481 catccgcgac ttcctgcgcg tgttcctgca gatgtcggtg gtgctcacct acgcggcggc
      541 cttgccggtg gtgaaggtcg gccgcatcgc cggccagttc gccaagccgc gcacctcgcc
      601 gatggagaag aaggacggca aggagctgcc gagctatcgc ggcgacgtca tcaacggcgt
      661 cgagttcacc gacgaggcac gccggcccga tccggcacgg cagatcgagg cattccgtca
      721 ctccgccgcc acgctgaacc tgctgcgcgc gttcgtgcag ggcggctacg ccaatctcgc
      781 caatgtgcgg caatggacgc tcggcttcgt caaggactcg cctcagtcgc accactacga
      841 ggagctcgct gatcgcatct ccgaggcgct cggcttcatg caggcctgcg ggctcgacct
      901 cgagagccat cccgagctca agaacaccga gttttacacc agccacgaag cgctgctgct
      961 cggttacgag caggcgatga cgcgcgagga ctcgacccat cccggcagct tctgctgcac
     1021 ctccgcccac atgttgtgga tcggcgaccg cacccgccag cccgatcatg gccatgtcga
     1081 gttctgccgc ggcatcatca atccgctcgg gctcaaatgc gggccctcga gcaagatcga
     1141 cgagatgctg cggctgatcg acatcctcaa cccggagaac gagccgggcc ggctgacgct
     1201 gatcatacgg ctgggctcgg acaaggtcgg cgatcagctt ccggccatga tccgcgcggt
     1261 gcagcgcgag ggcaaagtcg tgctgtggtc gtgcgacccc atgcacggca acaccatcac
     1321 ctcggagacc ggatacaaga cgcgaccgtt cgagcgcatc ctctcagagg tgaagacctt
     1381 cttcgcggtc caccgcgccg aaggcactca cggcggtggc gtgcatctgg agatgaccgg
     1441 ccagaacgtg gccgagtgca ccggcggcgc ccgcatgatc accgacgcgg acttcaagga
     1501 ccgttatcac accgtctgcg atccaaggct caacgccgag caggcgatcg atctggcgtt
     1561 cctgctggcg gacctgctca aggccgagcg caccgccaag gcgcggccgc tgccggctgc
     1621 cgccggtctt tgatcggacc actgcacctg gcgtcgccgg ggtttcatcc ccggcggtcg
     1681 cggaggttaa ccttcctcag gcgcgcatcc cgcgtgggcc ggggccgcgc tggatttagc
     1741 ggggcagggc gatagattgc gagccgagcc gacaccatca tcccccgacc tccgcctttc
     1801 gaatgcattc atggaacgac tgctgcgcgc caccatcaat acctggcacg gcctgctcgc
     1861 cgccgcgcgc agcgaggagg cctttcgcca ggaactggtc gccctcgttc tggcggtgcc
     1921 gctggcgttc ctgctggctg acgaggggtg gaagcggctg gcgctgatcg gtgtcatcgt
     1981 gttcgtgctc gtggtcgagc tgttgaacac ggcgatcgag aaacttgcgg atcgactgac
     2041 tcgcgaccat gacccgcaga tccgcgacgt caaggatatg gggtcggccg cggtcggcgt
     2101 cggcctcgcc atcgccgggc tgacctggct ggttgcgatt gccgaacggc tggggttcgt
     2161 ttgatcgcgc gattgcgcat tcgtggcgtc gcacactaca tactgtgatg attggtccga
     2221 aactgaaaac ctga
//
LOCUS       DASYJA010000452         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2883652, whole genome shotgun sequence.
ACCESSION   DASYJA010000452 DASYJA010000000
VERSION     DASYJA010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2883652"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VGO02_09875"
     CDS             <1..418
                     /locus_tag="VGO02_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004537916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HEV7478030.1"
                     /translation="VPAVYGWLSLDRRGSWRIKAERISNAALREFIARNYQADERGCW
                     YFQNGPQRVFVELAYTPLVVHYEDQALLDHCARPFSSRSFFQDDEGSVLVEGERGIAL
                     LDDRDLARLAERAESFPRIRRADVAQRYGFVAEPRP"
     gene            540..917
                     /gene="rpsL"
                     /locus_tag="VGO02_09880"
     CDS             540..917
                     /gene="rpsL"
                     /locus_tag="VGO02_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEV7478031.1"
                     /translation="MPTVNQLVRDGRVAKRAKSKVPALAGSPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGFEVIGYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGSL
                     DTQGVKDRKQSRSKYGAKAPKSA"
     gene            930..1400
                     /gene="rpsG"
                     /locus_tag="VGO02_09885"
     CDS             930..1400
                     /gene="rpsG"
                     /locus_tag="VGO02_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEV7478032.1"
                     /translation="MPRRREVPKREILPDPKFHNVEVAKFVNVLMTRGKKSVAERIIY
                     GALDTIKTKSGKDPIEVFTQAVQNVKPVVEVKSRRVGGANYQVPVEVRPVRRVALAMR
                     WIREAAQKRGEKSMMVRLAGELAEAAEGRGGAMKKREEVHRMAEANKAFSHYRF"
     gene            1423..>1610
                     /locus_tag="VGO02_09890"
     CDS             1423..>1610
                     /locus_tag="VGO02_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEV7478033.1"
                     /translation="MARKTPIERYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGIT"
BASE COUNT          322 a          501 c          542 g          245 t
ORIGIN      
        1 tgtccccgcg gtctacggct ggctgtccct cgatcggcgc ggcagctggc gcatcaaggc
       61 agagcggatc tccaatgcgg cgctgcgcga attcatcgct cgcaactacc aggcggacga
      121 gcgcggttgc tggtactttc agaacggccc gcaacgcgtt tttgtcgagc ttgcctacac
      181 cccgctggtg gtgcattacg aggaccaagc gctgctcgac cactgcgcgc ggcctttttc
      241 atcgcgcagc ttttttcagg acgacgaagg ctcggttctc gtggagggcg agcgcggcat
      301 cgcgctgctc gacgaccggg atctcgcgcg cctggcggag cgagccgagt catttccccg
      361 catccggcgc gcagacgtcg cgcagcgcta cggattcgtc gccgaacccc gcccgtaagg
      421 ggtgaaatgc gttgcgctga cccggaatcc tctttataat ccgcgctctt ttttagcccc
      481 tgggcggctg ctctgcgtac ccgagagccg cccggtttgt ttctggaaac cttaaaaaca
      541 tgcctaccgt caatcagtta gttagggatg ggcgcgtcgc caagcgcgcc aagtcgaaag
      601 tcccggcgct cgccggctcg ccgcaaaagc ggggagtctg cacccgggtc tatacgacga
      661 cgcccaagaa gccgaactcg gcgctgcgca aggtggcgaa ggtgcgcctg accaatggct
      721 tcgaggtcat cggctacatc ggcggcgaag gccacaacct gcaggagcac tcggtggtgc
      781 tgatccgcgg cggccgcgtc aaggacctgc cgggggtgcg ctaccacatc gtgcgcggct
      841 cgctcgacac gcagggcgtg aaagaccgca agcagagccg ttcgaagtac ggcgccaagg
      901 cgccgaagtc ggcctaagga cacgaacaga tgccacgccg cagagaagtc ccgaaacgcg
      961 agatcctgcc cgacccgaag ttccacaacg tcgaggtggc gaagttcgtc aacgtcctga
     1021 tgacgcgggg caagaagtcg gtggccgaac gcatcatcta cggcgcgctc gacaccatca
     1081 agacgaagtc cggcaaggac ccgatcgagg tgttcacgca ggcggtgcag aacgtgaagc
     1141 cggtggtcga ggtgaagagc cgccgcgtcg gcggcgccaa ctaccaggtg ccggttgaag
     1201 tgcggccggt acggcgcgtg gcgctggcga tgcgctggat ccgcgaggcg gcgcagaagc
     1261 gcggcgagaa gtcgatgatg gtgcgcctcg ccggcgagct cgccgaggcg gccgaaggcc
     1321 gcggcggcgc gatgaagaag cgcgaggaag tgcaccgcat ggcggaggcc aacaaggcct
     1381 tctcgcacta caggttctag gaaggatcga ggaagagtgg ccgtggcacg caagacccca
     1441 atcgaacgct accggaacat cggaatttcc gcgcacatcg acgctggaaa aaccacgacc
     1501 acggagcgga tcctgttcta caccggggtc aaccacaaga tcggcgaggt gcacgacggc
     1561 gccgcgacga tggactggat ggagcaggag caggagcgcg gcatcaccat
//
LOCUS       DASYJA010000453         4036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3065001, whole genome shotgun sequence.
ACCESSION   DASYJA010000453 DASYJA010000000
VERSION     DASYJA010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3065001"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1576
                     /locus_tag="VGO02_09895"
     CDS             <1..1576
                     /locus_tag="VGO02_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEV7478034.1"
                     /translation="LRWRMRENLPGEFPFTGGVFAFKRENEDPTRMFAGEGDPFRTNK
                     RFHLLSKDLPAKRLSTAFDSVTLYGFDPDERPDIYGKVGNSGVSIATLEDMKVLYSGF
                     DLCSPQTSVSMTINGPAPTILAMFFNTAIDQQVEKLGRKPTEKELQEIRAQTLSAVRG
                     TVQADILKEDQGQNTCIFSTEFSLKVMGDIQQYFIENDVRNFYSVSISGYHIAEAGAN
                     PISQLAFTLANGFTFVEAYLARGMDIDDFAPNLSFFFSYGMDPEYAVLGRVARRLWAV
                     AMKRRYGANERSQKLKFHSQTSGRSLHAQEIAFNDIRTTLQALISTYDNTNSLHTNAY
                     DEAITTPTEESVRRAMAIQMIINREWGVAKNENPNQGAFVMDELTDLVEEAVLKEFEA
                     ISSRGGVLGAMETGYQRGKIQEESLYYEHKKHDGSYPIIGVNTFRNPHGDSTPQKLEL
                     IRSTEDEKQSQLKRLREFHSENSAVSEEMLERLKRSVVEDRNVFAVLMDAVRVCSLGQ
                     ITHALFEVGGQYRRNM"
     gene            complement(1580..2869)
                     /locus_tag="VGO02_09900"
     CDS             complement(1580..2869)
                     /locus_tag="VGO02_09900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7478035.1"
                     /translation="MAIVASAFAGKWLVDNFSSPGDSPAAAAGGHFIDVREFKRTTTV
                     EQLAGTAEEYFARLKNWDFALAKPLWDAEGAPEQLNNFGHLLHGLQLLPGMAIVDFGA
                     GSCWASRWLTQMGMEAIALDVSPTALKIGRVLYERLPVIGNRPQPRFLVFDGRRIELG
                     DASVDRILCIDAFHHVLNQDEVLREMSRILKPGGIAGFSEPGPQHSMSPASQFEMRNF
                     RVLEDDIHIDEIWSSARQAGFRRIKLAVFNAPTYLLLSPSDFEDYLSGGEAAEQFGRV
                     TRLQMRGRRMFFLQKGGESPALDSRSRVGLRATLEVSLASTTVKQGGPLTAQVVATNS
                     GRATWLPGTSKVGGVLLTCNLLDTSGKMLQQDYFRYLLTPGDGRAIAPGESVKLEVRI
                     PPPAKGQYLLEFDLVSESVTFFSALGSETVQLAIQVV"
     gene            complement(2914..3849)
                     /locus_tag="VGO02_09905"
     CDS             complement(2914..3849)
                     /locus_tag="VGO02_09905"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEV7478036.1"
                     /translation="MSKILIVDDSETIRAVLALTVQAMKFEPVLADCGERALELFVAE
                     RPSLVLLDVNMPGIDGYETARRIRAVRQEEWIPIIFLSASEDDQDLKRAIECGGDDYL
                     VKPVSTVVLNAKMRALQRLDQMRRKLIEVSNELTAANQRLETLSHQDGLTGIANRRAF
                     DFLIERQFVEAQRRREPFAVVLCDVDHFKAYNDQYGHLAGDECLRQVAAALARSCKRA
                     TDVAARYGGEEFALLLPDTPAAGALRVVEIAQTELAALAITHAGSTTSNVVTFSAGIA
                     SYLPERDKVSRDLAARADEALYRAKQLGRNRAVAV"
BASE COUNT          767 a         1318 c         1265 g          686 t
ORIGIN      
        1 gctgcgctgg cgcatgcgcg agaacctgcc gggcgagttc cccttcaccg gcggcgtgtt
       61 cgcgttcaag cgcgagaacg aggacccgac gcgcatgttc gccggcgagg gcgatccgtt
      121 ccgcaccaac aagcgcttcc acctgctgtc gaaggacctg cccgccaagc gcctttcgac
      181 cgcgttcgac tcggtgacgc tctacggctt cgacccggac gagcgccccg acatctacgg
      241 caaggtcggc aactccggcg tgtcgatcgc cacgctcgag gacatgaagg tgctctactc
      301 gggcttcgac ctgtgcagcc cgcagacctc ggtgtcgatg acgatcaacg gcccggcgcc
      361 gaccatcctg gcaatgttct tcaacacggc cattgatcag caagttgaaa agctgggtcg
      421 aaaacccacg gaaaaagagc ttcaggaaat aagagcgcaa accctttcag ccgtgcgcgg
      481 cacggtgcag gccgacatcc tgaaggaaga ccagggacag aacacctgca tcttctccac
      541 cgagttctcg ctcaaggtga tgggcgacat ccagcagtac ttcatcgaga acgacgtgcg
      601 caacttctac tcggtgtcga tctcgggcta ccacatcgcc gaagccggcg ccaacccgat
      661 cagccagctc gccttcacgc tcgccaacgg cttcaccttc gtcgaggcgt atctggcgcg
      721 cggcatggac atcgacgact tcgcgccgaa cctgtcgttc ttcttctcgt acggcatgga
      781 cccggagtac gccgtgctcg ggcgggtggc gcggcgcctt tgggcggtgg cgatgaagcg
      841 ccgctacggc gccaacgagc gcagccagaa gctcaagttc cactcgcaga cctcgggccg
      901 ctcgctgcac gcgcaggaga tcgccttcaa cgacatccgc accacgctgc aggcgctgat
      961 ctcgacctac gacaacacca attcgctgca caccaacgcc tatgacgagg cgatcaccac
     1021 gccgaccgag gagtcggtgc gccgcgcgat ggcgatccag atgatcatca accgcgaatg
     1081 gggcgtggcg aagaacgaga acccgaacca gggcgcgttc gtgatggacg agctcaccga
     1141 cctggtcgaa gaggccgtct tgaaggagtt cgaggcgatc tcgagccgcg gcggcgtgct
     1201 cggcgcgatg gagaccggct accagcgcgg caagatccag gaggagtcgc tctactacga
     1261 gcacaagaag cacgacggct cgtatcccat catcggcgtc aacacgttcc gcaatccgca
     1321 cggcgactcg acgccgcaga agctcgagct gatccgctcc accgaggacg agaagcaatc
     1381 ccagctgaag cggctgcggg aatttcattc ggagaattcc gccgtttccg aggagatgct
     1441 ggaaagattg aagcgatcgg tagtagaaga ccgcaatgtg tttgcagtgt tgatggacgc
     1501 tgtgcgcgtc tgctcgctgg ggcagatcac ccacgctttg ttcgaggtcg gcgggcagta
     1561 ccggcgcaat atgtagcttt taaacgacct ggatcgccag ctggacggtc tccgagccca
     1621 acgcgctgaa aaaagtcacc gattcgctga ccaggtcgaa ctcgaggaga tactgtccct
     1681 tggcgggcgg cgggatgcgc acctcgagct tgacggattc ccccggtgcg atggctcgtc
     1741 cgtctcctgg agtgagcagg tagcggaaat agtcctgctg aagcatcttg ccggaggtat
     1801 ccagcaaatt gcacgtcagc aggaccccgc cgacttttga ggtgccaggc agccacgtcg
     1861 cccggccgct gttggttgcc acgacttgag cggtcagcgg gccgccttgc ttcaccgtgg
     1921 tcgaagccag gctgacctcc aaggtcgcgc gcaagccgac gcggctcctg ctgtccagcg
     1981 ccggggactc cccgcccttt tgcaggaaga acatccggcg gccccgcatc tgcaaccgcg
     2041 taaccctgcc gaactgctcg gcagcttctc cgccgctcag gtagtcctcg aagtcggacg
     2101 gactcaacag caaataggta ggagcgttga acaccgccag cttgatccgc cgaaaccccg
     2161 cctgccgggc ggaagaccag atttcatcga tgtgaatatc gtcctcgagg accctgaaat
     2221 tcctcatctc gaactgggat gccggagaca tcgagtgctg cggacccggc tccgagaacc
     2281 cggcaatccc gcctggcttg aggatccggc tcatctcccg cagcacctcg tcctggttga
     2341 gcacgtgatg aaacgcatcg atgcacagga tccggtcgac gctggcatcg ccaagctcga
     2401 ttcgacgtcc gtcgaaaacc aggaagcgcg gctgcggccg gttgccgatg acaggcaggc
     2461 gctcgtacag cacccggccg atcttgaggg cggtaggcga cacgtcgagc gcaatcgctt
     2521 ccatccccat ttgagtcagc cagcgcgatg cccagcacga gccggccccg aaatccacga
     2581 tggccattcc gggcaggagc tgcagaccgt gcagcagatg cccgaagttg ttcagctgct
     2641 cgggtgctcc ctcggcatcc cacagcggct tggcgagggc gaagtcccag ttcttgagcc
     2701 tcgcgaaata ctcttccgct gtcccggcaa gctgctcgac cgtcgtcgtt ctcttgaact
     2761 cgcggacgtc gatgaaatgc ccgccggcgg ccgccgcagg cgagtcgccg ggcgaggaaa
     2821 agttgtccac cagccacttg ccggcgaagg ccgaggcaac gatagccact gcgctctgaa
     2881 aaagtgtcct gcggttcaaa gcggtgcggc gtcctagacc gcgactgcgc gattgcggcc
     2941 gagctgcttt gcccggtaga gcgcctcgtc ggcccgcgcg gcgagatcgc gcgacacctt
     3001 gtcgcgctcg ggcaggtagc tcgcgatacc cgcgctgaac gtcaccacat tcgaggtcgt
     3061 cgagccggcg tgcgtgatgg cgagcgcggc aagctccgtc tgcgcaatct cgacgactcg
     3121 cagcgcgcct gcggcgggcg tgtcgggcag aagcagcgcg aactcctcgc cgccgtaccg
     3181 cgcggccacg tcggtggcgc gcttgcagct gcgcgcgagc gcagccgcta cctggcgcag
     3241 gcattcgtcc ccggcaaggt ggccgtattg atcgttgtag gccttgaagt ggtcgacgtc
     3301 gcagagcacc accgcgaacg gctcgcgccg gcgctgcgcc tcgacaaact gcctctcgat
     3361 caggaaatcg aaggcgcggc ggttggcgat gccggtcagg ccgtcctggt gagaaagcgt
     3421 ctccagccgc tggttcgccg cagtcagctc gttcgacacc tcgatcagct tgcggcgcat
     3481 ctggtcgagc cgctgcagcg cgcgcatttt ggcattcagt acaacggtgc tgacgggctt
     3541 tacaaggtag tcgtcgccgc cgcactcgat ggctcgcttc aggtcctggt cgtcctcgct
     3601 ggccgaaagg aagatgatcg gaatccactc ctcctgccgc accgcgcgaa tgcgccgggc
     3661 cgtttcgtag ccgtcgatgc ccggcatgtt gacgtcgagc agtaccaggc ttggccgctc
     3721 ggcgacgaac agctcgagcg cgcgctcgcc gcaatcggcc aggaccggct cgaacttcat
     3781 agcctgcacc gtaagggcca ggaccgcgcg gatggtttcc gaatcgtcga cgatgaggat
     3841 cttggacatg ggtgcgcgta ggggattctc ctacaactga ctagtgctgc gctgcgcgac
     3901 ctggatagca acggtcgcgc cccgcgactt ttgcacggta aagagcgtgg aacgcgtgcg
     3961 caatcaggcc ggcggcgcca ggcaggtggg cggggtgcaa tgcaattccc acgctcgccg
     4021 tcaccctgaa cgccag
//
LOCUS       DASYJA010000454         1967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_887483, whole genome shotgun sequence.
ACCESSION   DASYJA010000454 DASYJA010000000
VERSION     DASYJA010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887483"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..104
                     /locus_tag="VGO02_09910"
     CDS             <1..104
                     /locus_tag="VGO02_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HEV7478037.1"
                     /translation="QTLDQNLQELVQRNVISPAEARGKAANKDNFAG"
     gene            120..1256
                     /locus_tag="VGO02_09915"
     CDS             120..1256
                     /locus_tag="VGO02_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEV7478038.1"
                     /translation="MEREQATKFIYDLLRALLAKKGSDLFLTAGFPPAMKIDSKMTPV
                     SQQALTAQHTAMLVRAIMNDKQAAEFEAKKECNFAINPSGIGRFRVNCFVQQGMVGAV
                     LRVITTHIPSFEELKLPPVLKDVAMTKRGLVLFVGGTGSGKSTSLAAMIGYRNENSHG
                     HIITIEDPVEYVHPHKNCLITQREVGVDTDSWAAALKNTLRQAPDVILIGEIRDREVM
                     EHAVAFAETGHLALGTLHANNSNQAMDRIINFFPEERRQQLLMDLSLNLKAVISQRLI
                     PLREGKGRAAAVEVLLNSPLISDLIFKGEVHEIKEIMKKSRELGMQTFDQALFDLYEA
                     GRISYEDALRFADSTNEVRLNIKLNSKDAKGKDLTKGIEHLDIV"
     gene            1262..>1967
                     /locus_tag="VGO02_09920"
     CDS             1262..>1967
                     /locus_tag="VGO02_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEV7478039.1"
                     /translation="MTSTRNGLLSGPFFCAAAAGAVVLALAASAAAQTTIRVPLDHPT
                     IQAAINAAGNGDTVLVAPGTYLENISFLGKAITVTSEAGPETTIIDGNQADSVVKFIS
                     GEGRASVFSGFAVLNGRSTRSGGGIWIKFSSPTIAGNIIANNRACEGAGLAVDSGSPL
                     IERNTIINNLQAGCSGGGGGGISVRPNPATGPHPKTEILNNVISGNGVDSADGGGIRV
                     VRNTLIRGNLISGNFAS"
BASE COUNT          406 a          634 c          623 g          304 t
ORIGIN      
        1 tgcagacgct cgaccagaac ctgcaggagc tggtgcagcg caacgtcatc tctccggccg
       61 aagcacgcgg caaggcggcc aacaaggaca acttcgccgg atagcaaagg aaccgagcca
      121 tggaacgcga acaggcaaca aaattcatct acgacctgct gcgggcgctg ctcgccaaga
      181 agggctcgga cctcttcctc accgcgggct tcccgccggc gatgaagatc gacagcaaga
      241 tgacgccggt gtcgcagcag gcgctcaccg cgcagcacac ggcgatgctg gtgcgcgcga
      301 tcatgaacga caagcaggcg gccgagttcg aggcgaagaa ggagtgcaac ttcgccatca
      361 acccctccgg catcggccgc ttccgcgtga actgcttcgt ccagcagggc atggtcggcg
      421 cggtgctgcg cgtgattacc acccacatcc ccagcttcga ggagctgaag ctgccgccgg
      481 tgctgaagga cgtggcgatg accaagcgcg gcctggtgct gttcgtcggc ggcaccggct
      541 cgggcaagtc gacctcgctc gccgccatga tcggctaccg caacgagaac tcgcacggcc
      601 acatcatcac catcgaggac ccggtcgagt acgtgcaccc gcacaagaac tgcctgatca
      661 cgcagcgcga ggtcggggtg gacaccgact cgtgggcggc ggcgctgaag aacaccctgc
      721 gccaggcgcc cgacgtgatc ctgatcggcg agatccgcga ccgcgaggtg atggagcacg
      781 cggtggcgtt cgccgagacc ggccacctgg ccctgggcac gctgcacgcc aacaactcca
      841 accaggcgat ggaccgcatc atcaacttct tcccggaaga gcgccgccag cagctcctga
      901 tggacctgtc gctcaacctg aaggcggtga tctcgcagcg cctgatcccg ctgcgcgagg
      961 gcaagggccg cgccgcggcg gtggaagtgc tgctcaactc gccgctgatc tcggatctca
     1021 tcttcaaggg cgaggtgcac gagatcaagg agatcatgaa gaagtcgcgc gagctcggca
     1081 tgcagacctt cgaccaggcc ctgttcgacc tgtacgaggc agggcgcatc agctacgagg
     1141 acgcgctgcg cttcgccgat tccaccaacg aggtgcgcct caacatcaag ctgaacagca
     1201 aggatgccaa gggcaaggac ttgaccaagg gcatcgagca cctcgacatc gtctagtcct
     1261 cgtgacctcg acaagaaacg ggctgctgag cggccctttt ttttgcgcag ccgccgcggg
     1321 cgcggtcgtg ctcgccctcg ccgccagcgc tgctgcccaa accacgatca gggtgccgct
     1381 ggaccacccg acgatccagg ccgccatcaa cgcggccggc aacggcgaca cggtcctcgt
     1441 cgcgcctggc acgtacctcg agaacatcag cttcctgggc aaggcgatca cggtcaccag
     1501 cgaagcgggg ccggagacga ccatcatcga tggcaatcag gccgattcgg tcgtcaagtt
     1561 catttccggc gaaggccgcg cttcggtgtt cagcgggttt gcggtgctga acgggcgctc
     1621 gacgaggagc ggcggaggga tatggatcaa gttctcgtct ccaaccattg ccggaaacat
     1681 catcgcaaac aaccgggcgt gcgaaggggc tggattggca gtcgactcgg gctcgccgct
     1741 catagaacgg aacaccatta tcaataactt gcaggcgggt tgctccggag gcggcggcgg
     1801 ggggatttct gtccgcccga acccggcgac cgggccgcat ccgaagaccg aaattctcaa
     1861 caacgtaatc tccggcaacg gcgttgatag tgccgacgga ggagggatcc gggttgttcg
     1921 gaatacactc atccggggca atttgattag cggcaacttt gcttcag
//
LOCUS       DASYJA010000455         1689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1792850, whole genome shotgun sequence.
ACCESSION   DASYJA010000455 DASYJA010000000
VERSION     DASYJA010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1792850"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /gene="truB"
                     /locus_tag="VGO02_09925"
     CDS             complement(<1..129)
                     /gene="truB"
                     /locus_tag="VGO02_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012328383.1"
                     /note="catalyzes isomerization of specific uridines in RNA
                     to pseudouridine; responsible for residues in T loops of
                     many tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEV7478040.1"
                     /translation="MIDGGLLLDKPVGITSNRALQEAKKLLGARKAGHAGTLDPLAS"
     gene            complement(146..502)
                     /gene="rbfA"
                     /locus_tag="VGO02_09930"
     CDS             complement(146..502)
                     /gene="rbfA"
                     /locus_tag="VGO02_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEV7478041.1"
                     /translation="MKHSPGKKSGRTYKLGDLIQRELSELLQRELRDPRVGMITLTGV
                     DVSPDFSHAKVFYTTLDPAHVAEAAKGLKRAAGFLRSQLARRIKLYTTPELRFEYDES
                     VERGDRLSRLIDSTKQ"
     gene            complement(499..>1689)
                     /gene="infB"
                     /locus_tag="VGO02_09935"
     CDS             complement(499..>1689)
                     /gene="infB"
                     /locus_tag="VGO02_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEV7478042.1"
                     /translation="VALNKIDKHEANPEKVKQELLAHGVVPEEYGGDSPVVPVSAKTG
                     QGVDALLEQVLLQAEVLELRASPHAPAKGVVIEARLDKGRGPVATLLVQSGTLKRGDI
                     VLLGAVYGRVRAMLDENGKPVPEAGPSIPVEVQGLSEVPNAGEDMMVIADERKAREIA
                     SFRQGKFRDVKFAKQQATRLENMFGQMGEGEKKVLTLVIKSDVQGSQEALVHALTRLA
                     TEEVKVSVVHAGVGGITESDVNLAMAARAVIIGFNARADATARKLIESAGIQVRYYNI
                     IYDAVEEMKAALSGMLAPEKKESVLGMVQVRQVFRISKIGTVAGCYVQEGLVKRNAQV
                     RVLRDNVVIHTGEIDSLKRFKDDVREVKAGFECGMSLKNFSDLKEGDQFEVFEVVEVA
                     RSLS"
BASE COUNT          249 a          583 c          534 g          323 t
ORIGIN      
        1 cgaggcgagc ggatcgagcg tgccggcatg ccccgctttc cttgctccga ggagcttctt
       61 cgcttcctgc agggcgcggt tggaagtgat gcctaccggt ttgtcgagga gcaggcctcc
      121 atcgatcatt gcttgatgta gacgatcact gcttggttga gtcgatgagg cgtgagagcc
      181 ggtcgccgcg ctcgaccgac tcgtcgtact cgaagcgcag ctctggcgtg gtgtagagct
      241 tgatccgccg ggcgagctgc gagcgcagga agcccgctgc tcttttcaac cctttagcag
      301 cttcggccac gtgagccggg tcgagggtgg tgtaaaaaac cttggcgtgc gagaagtcgg
      361 gcgagacatc cacgccggtc agggtaatca tcccgacgcg cggatcgcgc agctcgcgct
      421 gcagcagctc ggagagctcg cgctggatga ggtcgccaag cttgtaggtc cgccccgatt
      481 tcttgccggg ggagtgtttc acgacagcga cctggcgacc tccacgacct cgaacacttc
      541 gaactgatcg ccttccttga ggtcggagaa gttcttcagc gacatgccgc actcgaagcc
      601 ggccttgacc tcgcgcacgt cgtccttgaa ccgcttgagc gagtcgatct cgccggtgtg
      661 gatgaccacg ttgtcgcgca gcacgcgcac ctgggcgttc ctcttgacca ggccctcctg
      721 cacgtagcag ccggccacgg tgccgatctt ggagatgcgg aacacctggc gcacctgcac
      781 catgccgagc accgactcct tcttctcggg ggcgagcatg ccggagagcg ctgccttcat
      841 ctcctcgacc gcgtcgtaga tgatgttgta gtagcgcacc tggatgccgg cggactcgat
      901 gagcttcctc gcggtggcgt cggcgcgcgc gttgaagccg atgatgactg cgcgcgcggc
      961 catcgcgagg ttgacgtccg actcggtgat gccgccgacg ccggcgtgca ccacgctcac
     1021 cttgacttcc tcggtggcga ggcgggtcag cgcgtgcacc agcgcctcct gcgagccctg
     1081 cacatcggac ttgatcacca gcgtcaggac cttcttctcg ccctcgccca tctgcccgaa
     1141 catgttctcg agcctcgtcg cctgctgctt ggcgaacttc acgtcgcgga acttgccctg
     1201 gcggaagctg gcgatctcgc gcgccttcct ctcgtcggca atcaccatca tgtcctcgcc
     1261 ggcgttgggc acctcggaca ggccctgcac ctcgaccggg atcgaaggcc cggcttccgg
     1321 caccggcttg ccgttttcgt cgagcatggc gcgcacccgg ccgtagaccg cgccgagcag
     1381 cacgatgtcg ccgcgcttga gcgtgccgga ttgcaccagc agcgtcgcga ccgggccgcg
     1441 gcccttgtcg aggcgcgcct cgatgaccac gcccttggcg ggcgcgtgcg gcgacgctct
     1501 gagctccagc acctcggcct gcagcagcac ctgctcgagc agcgcgtcca cgccctggcc
     1561 ggtcttggcc gacaccggca ccaccggcga gtcgccgccg tactcctcgg gcaccacccc
     1621 gtgcgcgagc agctcctgct tcaccttctc gggattggcc tcgtgcttgt cgatcttgtt
     1681 cagcgccac
//
LOCUS       DASYJA010000456         6889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2065914, whole genome shotgun sequence.
ACCESSION   DASYJA010000456 DASYJA010000000
VERSION     DASYJA010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6889
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2065914"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1557
                     /locus_tag="VGO02_09940"
     CDS             <1..1557
                     /locus_tag="VGO02_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV7478043.1"
                     /translation="DASITSTDAAGNVGAASDTESYTVDVTAPAPTVTLTSSITGDDI
                     INAAEAGGTVTITGSVGGAAAAGDTVTLTVNGNAYTGTVAAGNTFSINVAGADLAADA
                     DFTIDASITKADAAGNVGTGADTETYAVDVTVAAPTVAPLSTASVQPTLSGTFDAADT
                     AQLTVTVDGVTYLQGNPALTLSGNTWSLDLALAGQTLPAGAAYSVGVSATDNVGNSVS
                     NASAGVVTITAPATSTPPQPEEVVALSGGGTVTQQPPEPARPPAVAGLGDAPAGSAPS
                     TMPLGLPVDPTVSASNMGVRASVDQLLRPAAPAFDLNALPPTAAGLQDPFGFPVERVS
                     MNRANEIVLGRGELPLIGHYLFEYRGITELAADGRVPQDAFAHTDPAAIVRLSARLAD
                     GSPLPAWLRLDGRGSFVGVPPEGLEGTLEVEVVARDTEDREARTTFSLELDALRDAAA
                     AAAARDSVLGLDVDKDEANRAKLKAKGEKPAVRGTPTFSDQMRVAKAKADPLLDRILG
                     KGQEKPRPPR"
     gene            1706..3661
                     /locus_tag="VGO02_09945"
     CDS             1706..3661
                     /locus_tag="VGO02_09945"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEV7478044.1"
                     /translation="MLLAGCATTDPLSAEEREQLAADSRRQMFEGQEAVERPLSLAEA
                     TARAIKYQADHRQRRMEEAAAAAQLDVAKFDLLPRVTANAGYNYRNNDSFGFGFSPDG
                     TISTNPTASVERIYSTGGVGLAWNVLDFGVSYYRARQLSDQKLITEERRRKAVQTLMH
                     DVRIAWYRAEAAQRLLPNADMLLAEIDQAIEKTRVIEHGKLLPPVQTATLRRALLDLA
                     QQIALRRQELAQARVELATLINTPPGVEYKIVGADSDTREVLDLTADIDALELLALRN
                     RPEIAEEGYRARISRDEARKAMIGLMPNVSLDVGRNFDSNKFLLNNTWTSLGVRVALN
                     LVKVFSIPAMQRSEEAQKAADSARRQAMAMAVLAQTRIAAVRYTLVADEFMIWEEAAR
                     DDDLIVQHLGSSEKAGIDNELELIRARARALASRINRDLAYANVQASVARLYNSVGYD
                     AVPRDDEGKAVDELAQLIEARFGELESASFSKRSAAVKPSVAVGMVSGAQPRIGKLVQ
                     QGLAKALENAGFTSNDEPEVRLDLRLALDAPKDGVRTVSIRVSALPRGSANATLTREF
                     RTTLSEPVDDGQWRALGEGAVYKVSTDLATKRITRASLRIAERLKWPARRASAAKPAG
                     PPPAADPLPLRLDAALDPVAGRAGAGR"
     gene            3658..4515
                     /locus_tag="VGO02_09950"
     CDS             3658..4515
                     /locus_tag="VGO02_09950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV7478045.1"
                     /translation="MKRLLVEAGVIAACVVALLVACTEGPAPQPRAAPAAAVAPEAKP
                     QAVAPKPEPVVLKPEAPAKVEPVPGGEIAALILASEETVLSSQMAGRIRHVNVGLGDE
                     VKSGATLIEFDCGEQRAQLDAADADYRGARETHIARLKLQALGAAGELEVTVAAAAAD
                     KARSQVTLRRSQLAYCHVASPFRGRVARLRVKSSESVQSGQPLVDVVNPSVLKAQVFV
                     PAAWIVWLRPGGVVTINANGQPYAARISKLNSRVDGVSQQLELEALIENAEGLVPGMI
                     GVATFNPPK"
     gene            4512..5849
                     /locus_tag="VGO02_09955"
     CDS             4512..5849
                     /locus_tag="VGO02_09955"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEV7478046.1"
                     /translation="MSESPNPALALLEFERRIQSATSNREVAFRAVNDGSQVLRFDQA
                     ILWRWDVFSRPLIAAASGLADVSADSPYQQWLVRLVRAITPEPFEAPQALAAADLPEA
                     LVADAEDWCPAHLLHCPLRGPGGEALGGILFFRAEPLSEAERAAAEWVARSTGYGLWA
                     WRSDKHQLKRWLKNPLTWRTLGIGAAAVALIALIPVRLSALAPAEITPLRPIPVTSPV
                     DGVVREIVVKPNEVVKADQLLAVLDDTSLRNRLELATKALDIARADLQRATFKSFSDE
                     ASRLELQVLSARMQEKGAEVTFLQELLGKSKLYAPQGGLAIFPGQEEWRGRPVQVGER
                     VMLIADPSVIDVTIYLPPEDAVELEPGADVNLILHVDPLSSLDATIERASYEATLAPD
                     GTLAYVVRARLRSGQGLPRIGLRGTAKIYAGRVTLGYYLLRKPLAFVRRSLGV"
     gene            5877..6557
                     /locus_tag="VGO02_09960"
     CDS             5877..6557
                     /locus_tag="VGO02_09960"
                     /inference="COORDINATES: protein motif:HMM:NF017815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HEV7478047.1"
                     /translation="MPPIRPLLVLAAAALAAAGVIGSLDAQPRVERGFTTAVSERLIQ
                     AYSQRFGPLAVERLQAWKRYARARGAAPGTERDLLDEVNRLLNRIAFVEDLAHWGEED
                     YWATPAESVASNGADCEDYTIAKYFLLKELGVPIARLRLTYVKSVKLNQAHMVLAYYA
                     RPDAEPLVLDNLEDRVRPASQRPDLIPVYSFNDEEVWVEMRGRSGSPQQIRNWSALIA
                     RLERERRT"
     gene            6554..>6889
                     /locus_tag="VGO02_09965"
     CDS             6554..>6889
                     /locus_tag="VGO02_09965"
                     /inference="COORDINATES: protein motif:HMM:NF027763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapD/MoxY N-terminal periplasmic
                     domain-containing protein"
                     /protein_id="HEV7478048.1"
                     /translation="MKVRRPMTLGRQVTWVVSAIFFFALVGVQAIHLRSTHASLQRQL
                     ESLAQDAATSIGLSLGVLLGGSDVALAETVINPAFDRGHYERIEYLGANGERLVSRTL
                     GSVQGRYPGW"
BASE COUNT         1068 a         2443 c         2431 g          947 t
ORIGIN      
        1 gacgcgagca tcacgtcgac ggacgcggcg ggcaacgtgg gcgcggcgag cgacaccgag
       61 agctacacgg tggacgtgac ggcgccggcg ccgacggtga cgctgacctc gagcatcacg
      121 ggcgacgaca tcatcaacgc tgcggaagcg ggcggcacgg tgacgatcac cggcagcgtc
      181 ggcggagcgg ctgcggcagg cgacacggtg acgctgacgg tcaacggcaa cgcgtacacg
      241 ggcacggttg cggcaggcaa caccttctcg atcaacgtgg cgggcgcgga cctggcggcc
      301 gatgccgact tcaccatcga cgcctcgatc acgaaggcgg acgcggcggg gaacgtaggc
      361 acgggcgcgg ataccgagac ctatgcggtg gacgtgacgg tcgctgcgcc cacagtcgct
      421 ccgctgagca ccgccagcgt ccagccgacg ctgagcggga ccttcgatgc cgccgacacc
      481 gcgcaactca cggtcacggt cgacggcgta acctatctgc agggcaaccc ggccctcacg
      541 ctgagcggca acacctggag cctggatctg gcgcttgccg gccagacgct tccggccggc
      601 gccgcgtaca gcgttggcgt aagcgcgacg gacaacgtcg gcaactccgt gagcaatgcg
      661 agcgccggag tggtgacgat cacggccccg gcaacgagca cgccgccgca accggaggag
      721 gtcgttgcgc tgtccggcgg agggacggta acccagcagc cgcctgagcc ggccaggccc
      781 ccggccgtcg ccggcctggg cgatgctccc gcgggctctg cgccttcgac gatgcctctc
      841 ggcctgccgg tcgacccgac cgtcagcgct tcgaacatgg gtgtgcgcgc ctcggtcgac
      901 cagctgctca ggccggcggc gcctgccttc gatctgaacg cgctgccgcc gaccgcggcc
      961 ggcttgcagg acccgttcgg cttcccggtg gagcgggtgt cgatgaaccg tgccaacgag
     1021 atcgtcctcg gccgcggcga gctgccgctg atcgggcact acctcttcga gtaccgcggc
     1081 atcacggagc tcgctgccga cgggcgggtg ccgcaggacg cctttgccca caccgacccg
     1141 gcggcgatcg tgcgcctcag cgcccggctc gccgacggct cgccgttgcc ggcatggctg
     1201 cggctcgacg ggcgcggcag cttcgtcggc gtgccgccgg agggcctcga aggcaccctc
     1261 gaggtcgagg tcgtggcccg cgacacggaa gaccgcgagg cccgcaccac gttctccctc
     1321 gagctcgacg cgctgcgcga cgcggccgcg gcggctgccg ccagggactc cgtcctcggc
     1381 cttgatgtcg acaaggacga ggccaatcgg gcgaagctca aagccaaggg ggagaagccg
     1441 gccgtacgcg gcaccccgac gttcagcgac cagatgcggg tcgccaaggc gaaggccgac
     1501 cccctgctgg accggatcct tgggaagggc caggagaagc ccagaccccc ccgataaacc
     1561 tttacatgat gggtttttgg gggcgggtta tgatggcagt cccgccgact ttgggaccgg
     1621 cgcccagaaa taaaacatga ggttatcggg gcaacttcga ctactgtcgc agtgttgcag
     1681 cgcagcattc acggttgcgc tgtttgtgct gctcgcaggg tgcgcgacga ccgatccgct
     1741 cagcgccgaa gagcgcgagc agcttgcggc cgactcgcgc cggcagatgt tcgagggcca
     1801 ggaggctgtc gagcgtccgc tgtcgctcgc cgaagccacc gcgcgtgcga tcaagtacca
     1861 ggccgaccac cggcagcggc ggatggaaga ggccgccgcc gcggcgcaac tggacgtggc
     1921 gaagttcgac ctgctgccac gcgttaccgc gaatgccggc tacaactatc gcaacaacga
     1981 cagcttcggc ttcggcttct cgcccgacgg caccatctcc accaacccca ccgcgtcggt
     2041 ggagcgcatc tacagcaccg gcggcgtcgg cctggcgtgg aacgtgctcg atttcggcgt
     2101 cagctattac cgcgcgcggc agctttccga ccagaagctg atcactgagg aacggcgccg
     2161 caaggcggtg cagaccctga tgcacgacgt gcggatcgcg tggtaccgcg ccgaggccgc
     2221 gcagcgcctg ctgccgaacg ccgacatgct gctcgccgag atcgaccagg cgatcgagaa
     2281 gacccgcgtc atcgagcatg gcaagctcct gccgccggtg cagacggcaa cgctgcgccg
     2341 cgcgctgctc gacctcgcac agcagatcgc cttgcgccgg caggagctgg cgcaggcccg
     2401 ggtggagctc gccacgctga tcaatacccc gcccggggtg gaatacaaga tcgtcggcgc
     2461 cgacagcgat acgcgcgagg tcctcgatct caccgccgac atcgacgcgc tcgagctcct
     2521 cgcgctgcgc aaccggcccg agatcgccga ggaaggctac cgcgcccgca tctcgcgcga
     2581 cgaggcgcgc aaggcgatga tcggcctgat gcccaatgtg agcctcgacg tcgggcgcaa
     2641 cttcgacagc aacaagttcc tgctcaacaa cacgtggact tcgctcggcg tgcgcgtcgc
     2701 gctcaacctc gtcaaggtgt tctcgatccc ggccatgcag cgctcggaag aggcgcagaa
     2761 ggccgcggac agcgcgcgcc gccaggcgat ggcgatggcg gtgctcgcgc agacgcgcat
     2821 cgccgcggtg cgctacaccc tggtggccga cgagttcatg atctgggaag aggcggcgcg
     2881 cgacgacgac ctgatcgtgc agcacctggg ttccagcgag aaagccggca tcgacaacga
     2941 gctcgagctg atccgcgccc gcgcccgcgc gctggcgagc cgcatcaacc gcgacctggc
     3001 ctacgccaac gtgcaggcga gcgtcgcccg cctctacaac tcggtcggct acgacgccgt
     3061 gccgcgcgac gacgagggca aggcggtcga cgagctggcg cagctgatcg aggcgcgctt
     3121 cggcgagctg gagagcgcca gcttctcgaa gcgctccgct gccgtgaagc ccagcgtggc
     3181 ggtgggcatg gtgagcggcg cccagccgcg catcggcaag ctggtgcagc aggggctggc
     3241 aaaggcgctg gagaacgcgg gttttacttc gaacgacgag cccgaggtcc ggctcgacct
     3301 gcgcctcgct ctggatgcgc cgaaagacgg tgtgcgcacg gtcagcatca gggttagcgc
     3361 gctgccgcgg ggcagcgcga acgcgaccct gacccgggaa ttcaggacca cgctgagcga
     3421 gcccgtggac gatgggcagt ggcgtgcgct cggcgaaggc gcggtctaca aggtctccac
     3481 ggacctcgcc actaaacgca tcacgcgggc gtcgctgcgc atcgccgagc ggctcaaatg
     3541 gccggcgcgg cgtgcgtctg ccgcgaagcc ggcgggcccg ccgccagcgg cggatccgct
     3601 gccgctgcgc ctggatgcgg cgctcgatcc ggtcgccggg cgcgcaggag ccggtcgatg
     3661 aagcgccttc tcgtcgaggc cggcgtcatt gccgcgtgcg tggtggcttt gctcgtcgcc
     3721 tgcacggagg gcccggcgcc gcagcccaga gcggcacccg cggcggcagt cgcaccggag
     3781 gccaagccac aggcggtggc gcccaagccc gagccggtgg tcctcaagcc cgaggcgccg
     3841 gcgaaggtcg agccggtgcc cggcggcgag atcgcggcgc tgatcctggc gagcgaggaa
     3901 accgtcctgt cgagccagat ggccggaagg atccggcacg tcaacgtcgg cctgggcgat
     3961 gaggtcaaga gcggcgccac cctgatcgag ttcgactgcg gcgagcagcg cgcgcagctc
     4021 gatgcggcgg acgcggacta ccgcggggcg cgcgaaacgc acattgcgcg tctcaagctg
     4081 caggcgctcg gcgcggcggg cgagctcgag gtgacggtgg ccgcggcggc cgccgacaag
     4141 gcgcgcagcc aggtcacgct gcgacgctcg cagctcgcgt actgccacgt ggcatcgccg
     4201 ttccgcggcc gggtggcgcg cctgcgggtg aagagctccg agagcgtgca atccggccag
     4261 ccgctggtgg acgtggtcaa cccgtcggtg ctcaaggcgc aggtgttcgt gccggcggcc
     4321 tggatcgtct ggctgcgtcc cggcggggtg gtgaccatca acgcgaacgg ccagccctac
     4381 gcggcgcgca tctcgaagct gaactcgcgc gtcgacggcg tgagccagca gctcgagctc
     4441 gaggcgctga tcgagaacgc cgaggggctg gttccgggga tgatcggcgt ggcgaccttc
     4501 aacccgccca agtgagcgaa agtcccaatc ccgccctcgc gctgctcgag ttcgagcggc
     4561 gcatccagtc ggccacgtcc aaccgcgagg tggcgttccg cgccgtcaac gacggctccc
     4621 aggtgctgcg cttcgaccag gcgattctct ggcgctggga cgtgttttcg cgcccgctga
     4681 tcgccgccgc ctcggggctc gccgatgtct ccgccgacag cccctaccag cagtggctgg
     4741 tgcgcctggt gcgcgccatc acacccgagc cgttcgaggc gccgcaggcg ctcgccgctg
     4801 ccgacctgcc ggaggcgctg gtggccgacg ccgaggactg gtgtcccgcg cacctgctgc
     4861 attgcccgct gcgcgggccc ggcggtgaag cgctcggcgg catcctgttc tttcgcgccg
     4921 agccgttgtc ggaagccgag cgcgccgcgg ccgagtgggt cgcgcgctcg accggctacg
     4981 gcctgtgggc ctggcgcagc gacaagcacc agctcaagcg ctggctgaag aacccgctca
     5041 cctggcgcac gctcggcatc ggcgccgcgg cggtggcgct catcgctctg atcccggtgc
     5101 ggctgagcgc gctcgcgccc gccgagatca ccccgctcag gccgatcccg gtgacctcgc
     5161 cggtggacgg cgtggtgcgc gagatcgtgg tcaagcccaa cgaggtggtg aaggcggacc
     5221 agctcctcgc ggtgctcgac gacacgtcgc tgcgcaaccg cctcgagctc gccaccaagg
     5281 cgctcgacat cgcgcgcgcc gacctgcagc gcgccacctt caaatcgttc agcgacgagg
     5341 cgagccgcct cgagctgcag gtgctcagcg cccgcatgca ggagaagggc gccgaggtca
     5401 ccttcctgca ggagctgctc ggcaagtcga agctgtatgc gccgcagggc gggctcgcca
     5461 tcttcccggg ccaggaggaa tggcgcggcc ggccggtgca ggtcggcgag cgcgtcatgc
     5521 tgatcgccga cccctcggtg atcgacgtca ccatctacct gccgcccgag gacgcggtcg
     5581 agctcgagcc cggcgccgac gtcaacctga tcctgcacgt cgacccgctg tcctcgctcg
     5641 atgcgacgat cgagcgcgcg agctacgagg ccacgctggc gcccgatggc acgctcgcct
     5701 acgtggtgcg cgcgcggctg cgctcgggcc aggggctgcc gcgcatcggc ctgcgcggca
     5761 ccgccaagat ctacgccggc cgcgtcacgc tcggctacta cctgctgcgc aagcctctcg
     5821 ctttcgtccg ccgctcgctc ggcgtctgaa agcggggccg gcgccggcga cccgtcatgc
     5881 cgcccatccg gccgctcctc gtcctcgcgg ccgctgcgct cgccgccgcg ggtgtcatcg
     5941 gctcgctcga cgcgcagcca cgcgtcgagc gcggattcac caccgccgtg tccgagcgcc
     6001 tgatccaggc ctatagccag cgcttcgggc cgctcgccgt cgagcggctg caggcgtgga
     6061 agcgctatgc gcgcgcccgc ggcgcggcgc ccggcaccga gcgcgacctg ctcgacgagg
     6121 tcaaccgcct gctgaaccgc atcgctttcg tcgaggacct ggcgcactgg ggcgaggagg
     6181 actattgggc gaccccggcc gaatcggtcg ccagcaacgg cgccgactgc gaggactaca
     6241 ccatcgcaaa gtatttcctg ctgaaggagc tgggcgtgcc catcgcgcgg ctgcgcctga
     6301 cctacgtcaa gtcggtgaag ctcaaccagg cgcacatggt gctcgcctac tatgcccgcc
     6361 ctgacgccga gcccctggta ctggacaacc tggaagaccg cgtgcgcccg gcttcgcagc
     6421 gccccgacct gatcccggtc tacagcttca acgacgagga ggtgtgggtg gagatgcgcg
     6481 gccgcagcgg ctcgccgcag cagatccgca actggagcgc gctgatcgcg cgcctcgagc
     6541 gggagcggcg tacgtgaagg tgcgccgccc catgacgctc ggccggcagg tcacctgggt
     6601 ggtctccgcg atcttcttct tcgcgctggt cggcgtgcag gcgatccact tgcgcagcac
     6661 gcacgccagc ctgcagcgcc agctcgaatc gctggcgcag gacgccgcca cctcgatcgg
     6721 cctgtccctc ggcgtgctgc tcggcggcag cgacgtcgcc ctggccgaga cggtgatcaa
     6781 cccggctttc gaccgcgggc actatgagcg catcgaatac ctcggtgcga acggcgagcg
     6841 gctggtgagc cgcaccctgg gctcggtgca gggccgctac ccgggctgg
//
LOCUS       DASYJA010000457         3168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_613633, whole genome shotgun sequence.
ACCESSION   DASYJA010000457 DASYJA010000000
VERSION     DASYJA010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613633"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VGO02_09970"
     CDS             <1..517
                     /locus_tag="VGO02_09970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV7478049.1"
                     /translation="RDATRAGEVIHRLRSLLMRGLPQKVELRLGDLIADVMSLVEGEA
                     RDKGIALTSFVEEDVMPLRADRIQLQQVLLNLVLNAIEALGASSGPRTVEVRARLGDA
                     GVVVAVSDSGHGLDPQCVERVFEAFFSTKREGMGMGLAICRSIVEAHGGQIHARNNGW
                     GGATFEFTLPF"
     gene            579..968
                     /locus_tag="VGO02_09975"
     CDS             579..968
                     /locus_tag="VGO02_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7478050.1"
                     /translation="MFIAIIEDHAGLREAIAALLDAAGYPSRGFESAEAFLRSHENGE
                     AACLVLDERLPGIDGLELQHQLAGAGRRLPIIFISAHEDAGTGVPARALQEGALAFLR
                     KPFNDIEFLDVIGTAWREGVRPIPRYT"
     gene            1023..1445
                     /locus_tag="VGO02_09980"
     CDS             1023..1445
                     /locus_tag="VGO02_09980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7478051.1"
                     /translation="MEIQEPSFVAVIEDDRALREAIESLLGSAGFAATGFESAEEFLC
                     CAGDPCCVVVDWRLPRMSGLEMLQRLRAARNPVPVIVMSARDEEARQAANQLQGVMGA
                     VAFVRKPFQGEVLLQLVRAVADRAWTRTVKWRRGTSSP"
     gene            1488..1985
                     /locus_tag="VGO02_09985"
     CDS             1488..1985
                     /locus_tag="VGO02_09985"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV7478052.1"
                     /translation="MQVAYEDDAVVIRLDTGPSDQSVDSLARAIAAYLLQGREGARAP
                     LAAPSRPAGLVPEKLARVRAYIEEHIAEALPTERLAAAVFMSPFHFSRLFKLAAGEPP
                     HGYVTRRRVERAKILLAHATLPLVHVAAAVGFQTQGHFTEVFRRRTGTTPRRFRLASR
                     ISTEV"
     gene            1989..2564
                     /locus_tag="VGO02_09990"
     CDS             1989..2564
                     /locus_tag="VGO02_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEV7478053.1"
                     /translation="MATKLFRTWRMFALRGLAAVLFGVLALVWPGLTLLWLVALFAAF
                     ALLSGGASVVGALRLRKSGEHWWLVLLLGLASLAAGVIAIFHPDLTALVLVVLMGANA
                     IVTGALDIALAVRLRKLLRGEWVLALSGVVSIAFGVLVILFPAAGALALVWLISVYAI
                     VSGVLLLIAAWRARKAERDVPHGMPEGGVAA"
     gene            2564..>3168
                     /locus_tag="VGO02_09995"
     CDS             2564..>3168
                     /locus_tag="VGO02_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV7478054.1"
                     /translation="MSYRTILVHLDTGERRRQRLDLAFILAEKYDAHLVGFFGLEDLV
                     IPPAPEAAPMLLQSMLEVRRKAAAAAEQEFRDKMSKEGYAAKSEWLATLGDGFAELQR
                     QAKYADLVVAGQPERDGGVPPWFSRELVMAIGRPVLYVPRAGRFEDCGERVLVPWNAS
                     REAARALEDAVPFLQRASEAEVVTFDERDDRRLPDPDVGAFL"
BASE COUNT          428 a         1073 c         1131 g          536 t
ORIGIN      
        1 ccgcgacgcc acgcgcgcgg gcgaggtgat ccaccgcctg cggtctttgc tcatgcgcgg
       61 cttgccgcag aaggtcgagc tgcgcctcgg cgacctgatc gccgacgtca tgagcctggt
      121 ggagggcgag gctcgcgaca agggcatcgc gctcacctcg ttcgtcgaag aggacgtgat
      181 gccgctgcgc gccgaccgga tccagctgca gcaggtgctc ctcaacctgg tgctcaacgc
      241 gatcgaagcc ctcggtgcgt cgagcgggcc gcgcaccgtc gaggtccgcg cgcgcctcgg
      301 cgacgccggc gtggtcgtag cggtcagcga ctcgggccac ggtctcgatc cgcaatgtgt
      361 cgagcgcgta ttcgaggcct tcttcagcac caagcgcgag ggaatgggca tgggcctcgc
      421 catctgccgc tcgatcgtcg aggcgcacgg cgggcagatc cacgcgagga acaacggctg
      481 gggcggcgcc accttcgagt tcacgctgcc tttctagccc gtatacctag gtgcaattgg
      541 aagcccgccg gcgtgcaccg atgctttgcg gtggccgtat gttcatcgct atcatcgagg
      601 atcacgcagg cctgcgcgag gccatcgcgg ccctgctgga tgccgcgggc tacccctcgc
      661 gcggcttcga gtccgccgaa gcgttcctgc gcagccacga gaatggcgag gcggcctgcc
      721 tggtgctgga cgagcgcctg ccgggcatcg acggcctcga gctgcagcac cagctcgccg
      781 gcgccggcag gcggctaccc atcatcttca tcagcgcgca cgaggatgcc ggcacgggcg
      841 tgccggcgcg tgcgctgcag gaaggagcgc tcgcttttct ccgcaagccc ttcaacgata
      901 tcgagttcct ggacgtcatc ggaaccgcgt ggcgagaggg tgtgcggcct atacctaggt
      961 atacctaggt gcaatagcgt ggcctgctgt ggtgtttatc ttgcaatcca cgcgggtatg
     1021 ccatggagat ccaggaaccc tcttttgtcg cagtcatcga ggatgatcgc gccttgcgcg
     1081 aggcgatcga gagcctgctc ggctcggcgg gcttcgccgc gacgggcttc gagtcggcgg
     1141 aggagttcct gtgctgcgcc ggcgacccgt gctgcgtggt cgtcgactgg aggctgccac
     1201 gcatgagcgg cctcgaaatg ctgcagcggc tgcgcgcggc ccgcaatccg gtgccggtga
     1261 tcgtgatgag cgcgcgcgac gaggaagcgc gccaggcggc gaatcagttg cagggcgtca
     1321 tgggcgccgt tgcgtttgtc cgcaagcctt tccagggcga ggttctgctg cagctggtgc
     1381 gcgcggtcgc cgaccgcgcc tggacgcgca cggtgaagtg gcgtcgcgga acgtcatcac
     1441 cctaaggtgc aatatcgcgg ccgggcgccg gacgttaggc tgcgatcatg caagtcgctt
     1501 atgaggacga cgcagtcgtc atccggctcg acaccggtcc gagcgatcag tcggtcgact
     1561 cgctcgcgcg ggcgatcgcc gcttatctcc tgcaggggcg cgagggtgcg cgcgcgcctc
     1621 tcgcagcgcc ctcacggccc gcgggcctcg tccccgagaa gctggcaagg gtgcgcgcgt
     1681 acatcgagga gcacatcgcc gaggcgttgc cgaccgaacg cctggcggcg gcggtcttca
     1741 tgagcccgtt tcatttctcg cgcctgttca agctcgccgc gggcgagccg ccgcacggct
     1801 acgtcacgcg ccggcgcgtc gagcgggcga agatcctgct cgcccacgcc accctgcccc
     1861 tcgtgcacgt tgcggcggcg gtcggcttcc agacgcaggg acattttacc gaggtgttcc
     1921 gcaggcgcac cggcaccacg ccgcgccgct tccggcttgc cagccgcatt tccacggagg
     1981 tctgacccat ggcaaccaag ctttttcgaa cctggcggat gttcgccctg cgcggcctcg
     2041 cggccgtgct gttcggcgtt ctcgcgctcg tctggccggg gcttacgctg ctctggctgg
     2101 tggcgctgtt cgccgccttc gcgctgctga gcggcggcgc ttcggtcgtc ggcgcgctca
     2161 ggctgcgcaa gagcggcgag cactggtggc tggtgctgct cctcgggctc gccagcctcg
     2221 ccgccggcgt gatcgcgatc ttccacccgg acctcaccgc cctggtcctg gtcgtgctca
     2281 tgggcgccaa cgcgatcgtc accggggcgc tcgacatcgc gctcgccgtg cgcctgcgca
     2341 agctgctgcg cggcgagtgg gtgctggcgc tgtccggcgt ggtgtcgatc gcattcggcg
     2401 ttctggtgat cctgtttccg gcggccgggg cgctggcgct ggtctggctg atcagcgtgt
     2461 acgcgatcgt gagcggggtg ctgctgctga tagccgcctg gcgcgcgcgc aaggcggagc
     2521 gcgacgtccc gcacggcatg cccgaaggcg gggtcgcagc ctgatgagct accggacgat
     2581 cctcgtgcac ctcgacaccg gcgagcggcg ccggcagcgg ctcgacctcg cctttatcct
     2641 ggcggagaag tacgacgccc acctcgtcgg cttcttcggc ctggaggacc tggtcattcc
     2701 acccgcaccc gaagcggcgc cgatgctgct gcagagcatg ctcgaagtgc ggcgcaaggc
     2761 ggccgcggcg gcggagcagg agttccgcga caagatgagc aaggagggct acgcggcgaa
     2821 gtcggaatgg ctggccaccc tcggcgacgg cttcgccgag ctgcagcgcc aggccaagta
     2881 cgccgacctg gtggtcgccg gccagcccga gcgcgacggc ggcgtgccgc cctggttctc
     2941 gcgcgagctg gtgatggcga tcggccggcc ggtgctctat gtgccgcgcg cgggacgctt
     3001 cgaggactgc ggcgagcgcg tgctggttcc ctggaacgcc tcgcgcgaag cggcccgcgc
     3061 gctcgaggac gcagtgccgt tcctgcagcg cgccagcgag gcggaggtcg tcactttcga
     3121 cgagcgggac gaccggcggc tgccggatcc cgatgtcggc gccttcct
//
LOCUS       DASYJA010000458         1638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2429174, whole genome shotgun sequence.
ACCESSION   DASYJA010000458 DASYJA010000000
VERSION     DASYJA010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2429174"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VGO02_10000"
     CDS             complement(<1..402)
                     /locus_tag="VGO02_10000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7478055.1"
                     /translation="MKTALVTGAGRGIGRAAAAALVKSGTKVFGLDKDFSEPLTNIEP
                     IRFDLTDLNAIPKLITGLGRLDVLVNNAGIQAALPIDRYTDEARGRMLRINLEAPVEL
                     IRAASKGMAERGSGRIVNVASVAAYMAHTDLW"
     gene            complement(399..1340)
                     /locus_tag="VGO02_10005"
     CDS             complement(399..1340)
                     /locus_tag="VGO02_10005"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV7478056.1"
                     /translation="MKPAMAKVKRIEGVLAPVVTPFRKDYAPDAARFVRHCRWLLKSG
                     CAGLAVFGTNSEANSMSVAEKRALLEALVAGGVPASALMPGTGHCALSDSIEMTRAAV
                     ELGCAGVLMLPPFYYKGVSDEGLYRNFAEVIERVGDERLQLYLYHIPPVAQIAITLPL
                     IEKLLSKYPGLVAGVKDSSGDWSNTKAMLDAFAKEGFDVFAGSEVFLLDNMRHGGKGC
                     ITATGNINPGPIANVYRNWRSADAERLQAGISATRKIVQKQPMIPALKAAVAHFGNDR
                     EWNTCRPPLVELTAAQEETLIAELKAAGFGMPGLTSV"
     gene            complement(1342..>1638)
                     /locus_tag="VGO02_10010"
     CDS             complement(1342..>1638)
                     /locus_tag="VGO02_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEV7478057.1"
                     /translation="AKRDPALPDLPTIAEAGVKGYESGVWFGIMVPAATPRDIVMKLN
                     AAALQATKAPEFVKRMTDLGYNIIPGSPEQMAAMLKEELARWAPIVKASGAKVD"
BASE COUNT          249 a          546 c          555 g          288 t
ORIGIN      
        1 ccagaggtcg gtatgcgcca tgtacgccgc caccgaggcg acgttgacga tacgccccga
       61 accccgctcg gccatgccct tcgaggccgc gcggatcagc tcgaccggcg cctccaggtt
      121 gatgcgcagc atgcggcccc gcgcctcgtc ggtatagcga tcgatcggca gcgcggcctg
      181 gatgccggcg ttgttcacca gcacatcgag cctgccaagc ccggtaatca gctttgggat
      241 cgcgttcagg tcggtgaggt cgaaccggat gggctcgatg ttcgtcaggg gctcggaaaa
      301 atccttgtcg agaccgaata ccttggtccc ggacttcacc agcgccgcgg cagcggcgcg
      361 gccgatgccg cggccggcgc cggtgaccag cgccgtcttc acacggaggt gagacccggc
      421 ataccgaagc cggcggcttt gagctcggcg atgagcgttt cctcctgcgc cgcagtcagc
      481 tcgacgagcg gcggccggca ggtgttccac tctcgatcgt tgccgaagtg cgccactgcc
      541 gccttcagcg ccgggatcat gggctgcttc tgcacgatct tgcgcgtggc gctgatgccg
      601 gcctggaggc gctccgcgtc cgcggagcgc cagttgcggt agacgttggc gatcggtcca
      661 ggattgatgt tgcctgtggc ggtgatgcag cccttgccgc cgtggcgcat gttgtcgagc
      721 aggaatacct cgctgccggc gaacacgtcg aagccctctt tggcgaaggc gtcgagcatt
      781 gccttggtgt tggaccagtc gcccgacgag tccttcaccc cggccacgag cccggggtac
      841 ttcgacagca gcttttcgat gagcggcagc gtgatcgcaa tctgcgcgac cggagggata
      901 tggtagagat agagctgcag gcgctcgtcg cccacgcgct cgatcacttc cgcgaaattg
      961 cggtacaggc cctcgtcgga gacgcccttg tagtagaaag gcggcagcat cagcaccccg
     1021 gcgcagccca gctccaccgc ggcgcgcgtc atctcgatgg agtcggaaag cgcgcagtgc
     1081 cccgtgccgg gcatgagcgc cgaggccggc acgccgcccg cgaccagcgc ctcgagcaag
     1141 gctctctttt cggcgaccga catcgagttc gcctcgctgt tggtgccgaa gaccgcgagg
     1201 ccggcgcagc cgctcttcag gagccagcgg cagtggcgca cgaagcgcgc cgcgtcgggc
     1261 gcgtagtcct tgcggaacgg cgtgaccacc ggggcgagca cgccctcgat gcgcttcact
     1321 ttggccatgg ctggtttcat gtcagtctac ctttgctccg gaagccttca cgatcggcgc
     1381 ccagcgcgcg agctcctctt tcagcatggc cgccatctgc tcgggcgagc cgggaatgat
     1441 gttgtagccg agatcggtca tgcgcttcac gaactcgggc gccttggtgg cctgcagcgc
     1501 ggcggcgttc agcttcatga cgatgtcgcg cggcgtcgcg gccggcacca tgatgccgaa
     1561 ccacacgccc gattcgtagc ccttgacgcc tgcttcggcg atggtgggca ggtccgggag
     1621 cgccgggtcg cgcttggc
//
LOCUS       DASYJA010000459         2476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2974334, whole genome shotgun sequence.
ACCESSION   DASYJA010000459 DASYJA010000000
VERSION     DASYJA010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2974334"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VGO02_10015"
     CDS             complement(<1..338)
                     /locus_tag="VGO02_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV7478058.1"
                     /translation="MIKAAAPRKAPSRASAGSLELAKRVLQIEADAVRALIERIDERF
                     LAAVDLIHGRTGRVVVSGIGKSGHIARKIASTLASTGTPAYFVHPAEASHGDLGMVDS
                     GDVFIAISYSG"
     gene            357..2330
                     /locus_tag="VGO02_10020"
     CDS             357..2330
                     /locus_tag="VGO02_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEV7478059.1"
                     /translation="MDYTADSLPSVLILLASGVAAVVLCRILRLPAMVGYLATGLVLG
                     PHALGLVADREAIHTLAEFGVVFLMFSIGLEFSLPKLVAMRRVVFGLGLAQVAASVAI
                     VVAAALAAGASWQAGLALGGIAAMSSTAIVSRLLTERGALDSAHGREVIGVLLLQDLA
                     VVPLLVVIPALGEKPEVLGVAVAAALGKAALALILVVALGPRLMRAWLGVVARRRSNE
                     LFVLNVLLVTLLLAFLTDLAGLSMVLGAFLAGMLISETEYRFQVEEDIKPFRDVLLGL
                     FFVTVGMMLDLRLVFAQFALVAALFALLVAGKFALIALLSRAFGAAPGTALRVGLALA
                     QGGEFGFVLLPLAGAAGVVPEPLLQALLAAMILSMLATPLLIGASDRIAMRLTRSEWM
                     TRSLELHRIAARSIEVEKHVIILGYGRNGQRMARLLDAEGVRYVALDLDPERVREAAA
                     AGDTVVFADSSRREALIAAGIMRASAVAFTFADVAAALRVLTHVRALNASVPVIVRAR
                     DEADLERLTAAGATEVVPEAFESGVMLASHTMVLAGVPLSRVMRRVAQVRDEQYGLLR
                     GLFHGRSEDSPADTRTRLHAVMLDERASGVGRRLAELGLEDLGVQVRAVRRLGEKRKL
                     AADEAGALREGDVVVLLGAPEGIDAAESLLLRG"
BASE COUNT          277 a          857 c          909 g          433 t
ORIGIN      
        1 cctgagtacg agatggcgat gaacacgtcg cccgagtcga ccatgccgag atcgccgtgg
       61 ctcgcctcgg ccgggtgcac gaaataggcc ggcgtgccgg tgctcgccag ggtcgaggcg
      121 atcttgcgcg cgatgtggcc cgacttgccg atgccgctca ccacgacgcg gccggtgcgc
      181 ccgtggatca ggtcgaccgc ggcgaggaag cgctcgtcga tgcgctcgat cagcgcgcgc
      241 accgcgtcgg cttcgatctg caggacccgc ttcgccagtt cgagagaacc ggcgctggca
      301 cggctcggtg cctttctcgg agcggctgcc tttatcattg gcgccgagta tagcccatgg
      361 attacaccgc cgattccttg ccgtcagtgc tgattctgct cgcgagcggc gtcgcggcgg
      421 tcgtgctgtg ccgcatcctg cgcctgccgg cgatggtcgg ctacctcgcg accggcctgg
      481 tgctcggacc gcacgcgctc ggccttgtcg ccgaccgcga ggcgatccat acgctcgccg
      541 aattcggcgt cgtctttctg atgttctcga tcggcctgga attcagcctg ccgaagctgg
      601 tcgcgatgcg ccgcgtggtg ttcggcctcg gtctggcgca ggtcgccgcc agcgtagcga
      661 tcgtggtggc tgccgcgctc gccgccggtg cctcctggca ggcgggcctc gccctgggcg
      721 gcatcgcggc gatgtcctcg accgccatcg tgtcgcggtt gctcaccgag cgcggcgcgc
      781 tggacagcgc gcacggccgc gaggtgatcg gcgtgctgct gctgcaggac ctcgccgtgg
      841 tgccgctgct ggtggtgatt ccggccctgg gcgagaagcc cgaggtgctc ggcgtcgctg
      901 tcgccgcggc gctgggcaag gcggcactcg cgctcatcct cgtggtggcg ctcggcccgc
      961 ggctgatgcg cgcctggctc ggcgtggtcg cccggcgccg ctcgaacgag ctgttcgtgc
     1021 tgaacgtgct gctggtgacg ctgctgctcg ccttcctcac cgacctcgcc ggcctgtcga
     1081 tggtgctcgg cgcgttcctc gccggcatgc tgatctcgga gactgaatac cgcttccagg
     1141 tcgaagagga catcaagccg ttccgcgacg tgctgctcgg cctgttcttc gtcaccgtcg
     1201 gcatgatgct cgacctgcgc ctggttttcg cccagttcgc tctggtggcg gcgctcttcg
     1261 cgctgctggt tgccggcaag tttgcgctga tcgcgctcct gtcgcgcgcc ttcggcgcgg
     1321 cgcccggcac ggcgctgcgc gtcggcctgg cgctcgcgca gggcggcgag ttcggcttcg
     1381 tgctgctgcc cctcgccggc gcggccggcg tggtgccgga gccgctcctg caggcgctgc
     1441 tcgcggcgat gatcctgtcg atgctcgcca cgccgctgct catcggcgcc tcggaccgca
     1501 tcgcgatgcg cctgacgcgc tcggaatgga tgacgcgctc cctcgagctg caccgcatcg
     1561 cggcgcgctc gatcgaggtc gagaagcacg tcatcatcct gggctacggc cgcaacgggc
     1621 agcgcatggc gcggctgctc gacgccgagg gcgtgcgcta cgtcgcgctc gacctcgacc
     1681 cggagcgcgt gcgcgaagcc gccgccgccg gcgacacggt ggtgttcgcc gacagctcgc
     1741 ggcgcgaggc gctgatcgcc gccggcatca tgcgcgcctc ggcggtggcg ttcaccttcg
     1801 ccgatgtcgc cgcggccctg cgcgtgctga cgcacgtgcg cgcgctcaac gcgtcggtgc
     1861 cggtgatcgt gcgcgcgcgc gacgaggcgg acctcgagcg gctcactgcc gcaggcgcca
     1921 ccgaggtggt gccggaggcg ttcgagtcgg gcgtgatgct cgcctcgcac accatggtgc
     1981 tcgccggcgt gccgctgtcg cgcgtcatgc gccgcgtcgc gcaggtgcgc gacgagcagt
     2041 acgggctgct gcgcgggctg ttccatggcc gcagcgagga ctcgcccgcc gacacgcgca
     2101 cgcggctgca cgccgtgatg ctggacgagc gcgcctcggg cgtggggcgg cggctcgccg
     2161 agctcggcct ggaggatctg ggcgtgcagg tacgcgcggt gcgccgcctg ggcgagaagc
     2221 gcaagctcgc ggcggacgag gcgggcgcgc tgcgcgaggg cgacgtggtg gtgctgctgg
     2281 gcgccccgga aggcatcgac gcggccgaaa gcctcctgct ccgcggataa aaaaagccgg
     2341 cgcaaggccg gctttttttc gtactgagcg aattagctta gagcgcgcgg cagatcgtgt
     2401 agacgttgct gccggtttcc tgatattgcg cggtggcaac ctggcctgcg ccgatggctg
     2461 ggttgggtcc ggcctg
//
LOCUS       DASYJA010000460         1599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2701875, whole genome shotgun sequence.
ACCESSION   DASYJA010000460 DASYJA010000000
VERSION     DASYJA010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2701875"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="VGO02_10025"
     CDS             <1..1322
                     /locus_tag="VGO02_10025"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7478060.1"
                     /translation="LHDEGIVKVSGEVGSGKTMLCRVLMERLPAHVETIYLATPSLAR
                     DEILHAIAEDLDLKLSGERTATALRELQEQLIRLYAAGRRVVVLVDEAHVMPEETLEQ
                     VRLLSNLESNRHKLLQMVLFGQPELDATLAKPSLRQLRDRITHSFRMRPLAAPEVTNY
                     VSFRMRAAGYRGPEVFAARALALVARASAGLTRRINILSDKALLAAFTENTHAVTDKH
                     VRAAIADSEFAAVTRVRRPVIYAGAALLAGAVLGAALAWGFFASRTEPAAPARAAAPA
                     PQPAAQPAAQPAAQPTTPAPEKTAEPVPPPAAPAPPSRLSEAQASRLAGYQPGRQPLL
                     AQRLAATRELLGRAADQSYAIELFITGNTDPARMERFLLRARELVRLDELAVIPMAEG
                     GQYRLRVVYGEFASREAALEAERRLPARYQNAFRTSLRSFADLRGQI"
     gene            1394..>1599
                     /locus_tag="VGO02_10030"
     CDS             1394..>1599
                     /locus_tag="VGO02_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478061.1"
                     /translation="MKPGISAGLSLCAQLCCGALLLAGCAQTPVKPAATHLRAPPPSA
                     EGQIPPPVQVAPLLPKPRPAGRVET"
BASE COUNT          213 a          606 c          561 g          219 t
ORIGIN      
        1 tcctgcacga cgagggcatc gtcaaggtat cgggcgaggt gggcagcggc aagaccatgc
       61 tgtgccgcgt gctgatggag cgcctgccgg cgcacgtcga gaccatctac ctcgccacgc
      121 cctcgctggc gcgcgacgag atcctgcacg ccatcgccga ggatctggat ttgaagctgt
      181 cgggcgagcg caccgcgacg gcgctgcgcg agctgcagga gcagctgatc cgcctgtacg
      241 ccgccggccg gcgcgtggtg gtgctggtgg acgaggcgca cgtcatgccc gaggagacgc
      301 tcgagcaggt gcgcctgctc tccaacctcg agtcgaaccg ccacaagctg ttgcagatgg
      361 tgctgttcgg ccagcccgag ctcgacgcca cgctcgccaa gccctcgctg cgccagctgc
      421 gcgaccgcat cacccactcg ttccgcatgc ggccgctcgc cgcgccggag gtgacgaact
      481 acgtgtcgtt ccgcatgcgc gcagccggct accgcggccc ggaggtgttc gccgcgcgcg
      541 cgctcgcgct cgtcgcccgc gcttcggccg gcctcacccg gcgcatcaac atcctgtccg
      601 acaaggcgct gctcgccgcc ttcacggaaa acacccatgc ggtcaccgac aagcacgtgc
      661 gcgcggcgat cgccgattcg gagttcgccg cggtgacgcg cgtgcgccgg ccggtgatct
      721 acgccggggc ggcgctgctt gccggggcgg tcctcggcgc cgcgctcgcg tggggcttct
      781 tcgccagccg caccgaaccg gccgcgcccg ctcgggcggc ggctcccgcg ccgcaacccg
      841 ccgcgcagcc tgcggcacag cccgcagcac agccgactac gccggcgccg gaaaaaaccg
      901 ccgagccggt gccgccgccg gcagcgcccg cgccgccttc caggctgagc gaggcgcagg
      961 cgagccggct ggccgggtat cagccggggc gccagcccct gctcgcgcag cgcctggccg
     1021 ccacgcgcga gctgctcggg cgcgcagccg accagagcta tgcgatcgag ctgttcatca
     1081 ccgggaacac cgacccggcg cgcatggagc gcttcctgct gcgtgcccgc gagctggtgc
     1141 gcctcgatga gctcgccgtg atcccgatgg ccgagggcgg ccagtatcgt ctgcgcgtcg
     1201 tctacggcga attcgcgagc cgcgaggcgg cgctcgaggc cgagcggcgc ctgccggctc
     1261 gctaccagaa cgcatttcgc acctcgttgc gcagttttgc cgatctgcgc ggtcaaattt
     1321 agtcagaccc gcagtcctgc cgagggtcgc gccctccccc ggatgctatg cttgccgcag
     1381 gagggagcat gcgatgaaac cggggattag cgccggcctg tcgttgtgcg cgcagctgtg
     1441 ctgcggcgcg ctgctgctcg ccggctgcgc ccagacgccg gtgaagccgg cggcgacgca
     1501 cctgcgcgcc ccgccgccca gcgccgaggg ccagattccg ccgccggtgc aggtggcgcc
     1561 gctgctgccc aagccccggc cggccgggcg cgtcgagac
//
LOCUS       DASYJA010000461         2316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3247978, whole genome shotgun sequence.
ACCESSION   DASYJA010000461 DASYJA010000000
VERSION     DASYJA010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3247978"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1166)
                     /locus_tag="VGO02_10035"
     CDS             complement(144..1166)
                     /locus_tag="VGO02_10035"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV7478062.1"
                     /translation="MPSIRQNAHSDVLPDFRNLGVIARVLLAANIAAIAAALLVARDL
                     AQGAAHFVQSASVLEPLLLACVLALAAASPLLGRLVYWQGCAAVLAIVLALTAAAQAA
                     IGALPGTQPDLARMLALSGLATAVLLVYFRLLAKAYSPALTEARLQALQARIRPHFLF
                     NSLNMVLSLVRRDPRRAERALEDLSDLFRTLMSEPRQFVRLAEEIELLQRYAELEQLR
                     LGERLQIDWELDTAPLDALLPPLVLQPLLENAVHHGVEPGTGPGVVLVRIERRGDRVF
                     ARIENPYIEEQQRRAGNRMALDNIRERLLLFFDAEARIRTSAADSRYRVEIEMPYRIA
                     ARTETA"
     gene            1204..>2316
                     /gene="argH"
                     /locus_tag="VGO02_10040"
     CDS             1204..>2316
                     /gene="argH"
                     /locus_tag="VGO02_10040"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV7478063.1"
                     /translation="MPKKAQPWSGRFAQPVDEQVKRFTASVSFDRRLAKHDIAASLAH
                     AQMLAAQGNLAKRDLAAIQKGLAKIAREVAAGTFAWSLDDEDVHLNIEKRLTALVGEA
                     GKRLHTARSRNDQVATDLRLWLRDEMDLIDKHLAALERALVEQASRHAALIMPGFTHL
                     QVAQPVTFGHHLLAYFEMLERDRERLFECRKRVNRLPLGAAALAGTSFPIDRARVARE
                     LGFEGLCENSLDAVSDRDFAIEFCACASLVMVHLSRLSEELVLWASPRFGFVRLPDAY
                     CTGSSIMPQKKNPDVPELVRGKSGRVFGHLVALLALMKGQPLAYNKDNQEDKEPLFDT
                     VDTVKDTLAIFAEMMAGLKANAKAMRAAALEGFSTAT"
BASE COUNT          381 a          784 c          798 g          353 t
ORIGIN      
        1 cgtgggaacg cgctcgagcg cctccaggcc atggcgcgcc tcgccgacga cgctggtcgc
       61 cacttcggcg gcgatgtcgc cgagcagctc cttcaggcgg ctgcgcgccg gctcctcgtc
      121 gtcgacgatg aacacgcgca ggctcatgcc gtctccgtgc gcgccgctat cctgtacggc
      181 atctcgatct ccacgcgata gcgcgagtcg gcggcgctgg tgcggatgcg cgcctcggcg
      241 tcgaagaaca gcagcagccg ctcgcggatg ttgtcgagcg ccatgcggtt gccggcgcgg
      301 cgctgctgtt cctcgatgta gggattttcg atgcgcgcga agacgcggtc gccgcgccgc
      361 tcgatgcgca ccagcacgac gcccgggccg gtgccaggct cgacgccgtg gtgcacggcg
      421 ttctcgagca gcggctgcag cacgagcggc ggcagcagcg cgtccaaggg cgccgtatcg
      481 agctcccagt cgatctgcag ccgctcgccg aggcgcagct gctcgagctc ggcgtagcgc
      541 tgcagcagct cgatttcctc ggccaggcgc acgaactgcc tcggctccga catcagtgtg
      601 cggaacaggt ccgacaggtc ctccagtgcg cgctcggcgc gccgcggatc gcgccgcacc
      661 agcgacagca ccatgttcag gctgttgaac agaaagtgcg gccggatgcg cgcctgcagc
      721 gcctgcagcc tcgcctcggt gagcgccggc gaatacgcct tcgccagcag gcggaagtac
      781 acgagcagca ccgccgtcgc gagcccgctc agcgccagca tgcgcgcgag atcgggctgc
      841 gttccgggca gcgcgccgat tgcggcctgc gctgcggcgg tgagggcgag cacgatcgcg
      901 agcaccgcgg cgcagccctg ccagtacacc agccggccga gaagcggcga cgccgcggcg
      961 agcgcgagca cgcaggcgag cagcaacggc tcgagcacgc ttgcgctctg cacgaagtgg
     1021 gcggcgccct gcgcaagatc gcgcgccacc agcagcgcgg cggcaatcgc cgcgatgttc
     1081 gcggcgagca gcacgcgcgc gatcaccccg aggttgcgga agtcggggag aacgtcggag
     1141 tgggcgtttt gccttatact cggcatactt agggggcggt gttgagccgc attctgaaac
     1201 attatgccga agaaggcgca accctggtcc ggccgcttcg cgcagcccgt agacgaacag
     1261 gtcaagcgct ttaccgcttc cgtctcgttc gatcggcgtc tggcgaagca tgacatcgcg
     1321 gcgtcgctcg cgcacgcgca aatgcttgcc gcccagggaa atctcgccaa gcgggatctg
     1381 gcggcgatcc agaaggggtt ggcgaagatt gcccgcgaag tggcggcggg cacctttgcc
     1441 tggtcgctcg acgacgagga cgtccacctc aacatagaaa agcggctgac ggcgctggtt
     1501 ggggaagccg gcaagcgcct gcacaccgcg cgctcgcgca acgaccaggt ggcgaccgac
     1561 ctgcgcctgt ggctgcgcga cgagatggat ctcatagaca agcacctcgc cgctctcgag
     1621 cgcgcgctgg tcgagcaggc ttcgcgccat gcggccctca tcatgccggg atttacccac
     1681 ctgcaggtag cgcagccggt gactttcggc catcacctgc tggcctattt cgagatgctg
     1741 gaacgggacc gcgaacgttt atttgaatgc agaaaaagag tgaaccgcct gccgctgggg
     1801 gccgcggccc ttgccggaac cagcttcccg atcgaccgcg cgcgcgtggc gcgcgagctc
     1861 ggcttcgagg gcctgtgcga gaactcgctc gacgcggtct ccgaccgcga cttcgccatc
     1921 gagttttgcg cctgcgcctc gctcgtcatg gtgcacctgt cgcgcctgtc ggaggagctg
     1981 gtgctgtggg ccagcccgcg cttcggcttc gtgcggctgc ccgatgccta ctgcaccggc
     2041 tcgtccatca tgccgcagaa gaagaatccg gacgtgccgg agctggtgcg cggcaagagc
     2101 ggccgggtat tcggtcacct ggtggcgctg ctcgccctga tgaagggcca gccgctggcc
     2161 tacaacaagg acaaccagga ggacaaggaa ccgctgttcg acaccgtcga cacggtgaag
     2221 gacacgctgg ccatcttcgc cgagatgatg gccgggctga aggccaatgc caaggcaatg
     2281 cgcgcagcgg cgctggaggg cttttccacc gccacc
//
LOCUS       DASYJA010000462         2422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_796375, whole genome shotgun sequence.
ACCESSION   DASYJA010000462 DASYJA010000000
VERSION     DASYJA010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796375"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="VGO02_10045"
     CDS             complement(<1..1127)
                     /locus_tag="VGO02_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEV7478064.1"
                     /translation="MAVREGSLEAPTRHPLDWRNPDFYDPAEVEKELERVFGICHGCR
                     RCVNLCTAFPTLFDLVDASSTFEVDGVAKQDFAKVVDQCYLCDMCYMTKCPYVPPHPW
                     NVDFPHLMLRAKAVKFRNGEASLRDKLLTSTDAMGKLAAIPVVAQTVNALNRNAFSRS
                     LMQAALGVHKDRDLPPFAPRKFRSAAEDSKTYQVRNGKLTPGKVAIFSTCYVNYNEPG
                     MGHDLLAILAHNEIPYVLVEQEACCGMPKLELGDLVSVSKLKEKNIPTLAKYAREGYA
                     ILTPIPSCTLMFKQELPLMYSDDADTIAVRDAMFDPFEYFVARRKDGLLKINFVRKLG
                     KVSYHIPCHSRVQNMGQKTREMLEAVPGTQVTTVERCAGHDG"
     gene            complement(1144..1566)
                     /locus_tag="VGO02_10050"
     CDS             complement(1144..1566)
                     /locus_tag="VGO02_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEV7478065.1"
                     /translation="MKALKGSKTHDNLKAAFAGESQANRRYLYFAAKADVEGQNDVAA
                     LFRSTAEGETGHAHGHLEYMEPVGDPATGLPIGRSRDNLKAAVAGETHEYTDMYPGMA
                     KEARGEGFAEIADWFETLAKAERSHANRFQKALDALSD"
     gene            complement(1653..1817)
                     /locus_tag="VGO02_10055"
     CDS             complement(1653..1817)
                     /locus_tag="VGO02_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478066.1"
                     /translation="MKTLITLFALLALVACHEGPAEKAGRKVDNAADKAGRSIEKAGE
                     NIQDAARGKK"
     gene            1932..>2422
                     /locus_tag="VGO02_10060"
     CDS             1932..>2422
                     /locus_tag="VGO02_10060"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEV7478067.1"
                     /translation="MISRIAITAALATLGGAAVAQTAQWDSGYVLDTNRQAARGAFNQ
                     CVRTGYWNESLAAEPCDATPRAGLAPVPVAQAAPAPAPVVAAPLAAPRPVLEKVTLSS
                     DVLFEFGKATLRDSGKQKLDELAGRLGGANVEEVVAVGHADRIDSEARNQRLSEQRAE
                     AVKV"
BASE COUNT          387 a          784 c          785 g          466 t
ORIGIN      
        1 ccgtcgtgcc cggcgcagcg ctcgaccgtc gtcacctggg tgccgggcac cgcctcgagc
       61 atctcgcgcg tcttctgccc catgttctgc acgcgcgagt ggcaggggat gtgataggag
      121 acttttccca gttttcttac gaaattgatc ttcaacaacc cgtctttccg ccgcgccacg
      181 aagtattcga acggatcgaa catggcgtcg cgcaccgcga tcgtgtcggc atcgtccgaa
      241 tacatgagcg gcagctcctg cttgaacatc agcgtgcagg acgggatcgg cgtgagaatg
      301 gcgtatccct cgcgggcgta tttcgccagc gtagggatgt ttttctcttt tagcttggaa
      361 acggaaacca aatcgccgag ctcgagcttg ggcatgccgc agcacgcctc ctgctcgacc
      421 aggacgtaag ggatctcgtt gtgagccagg atcgccagca ggtcgtggcc catccccggc
      481 tcgttgtagt tcacgtagca ggtactgaag atcgcgacct taccgggtgt gagcttgccg
      541 ttccttactt gatatgtctt tgaatcttct gctgcgcttc tgaattttcg cggcgcaaag
      601 ggcggcagat cgcggtcctt gtgaaccccg agggccgcct gcatcagact tctggaaaaa
      661 gcattcctgt tgagcgcgtt gaccgtctgc gcgaccaccg ggatggcggc gagcttgccc
      721 atcgcgtcgg tggacgtgag cagcttgtcg cgcaagcttg cctcgccgtt cctgaacttc
      781 accgctttcg cgcgcagcat gaggtgcggg aagtcgacgt tccacggatg cggcggcacg
      841 tacgggcact tcgtcatgta gcacatgtcg cacaggtagc actggtcgac taccttggcg
      901 aagtcctgct tggcgacgcc gtcgacctcg aaggtggacg aggcgtccac caggtcgaac
      961 agcgtgggga aggcggtgca caggttgacg caccgccggc agccgtgaca gatcccgaag
     1021 acgcgctcga gctccttctc gacctcggcc ggatcgtaga aatccgggtt cctccagtcg
     1081 agcgggtgcc gcgtcggagc ttcgagcgag ccttcgcgta cggccactgc gggggggtcg
     1141 cgcctagtcc gacagcgcgt cgagcgcctt ctggaagcgg ttggcgtggc tgcgctcggc
     1201 cttggccagc gtctcgaacc agtcggcgat ctcggcgaag ccctcgccgc gcgcctcctt
     1261 cgccatcccc gggtacatgt cggtgtactc gtgcgtctcg ccggccaccg ccgccttcag
     1321 gttgtcgcgc gagcggccga tcggcagccc cgtcgccggg tcgcccaccg gctccatgta
     1381 ctccaggtgc ccgtgcgcgt gccccgtctc gccctcggcg gtcgagcgga acagcgccgc
     1441 cacgtcgttc tggccttcaa cgtccgcctt cgcggcgaaa tacaggtaac ggcggttggc
     1501 ctgcgattcg ccggcgaacg ccgccttcag attgtcgtgc gtcttgctgc ctttgagcgc
     1561 tttcatggag cctcctcgta ggaagcaccg agcgtaggcc cctgcgccta atagatcaaa
     1621 tcaaaattgg caatcctgcc aattgaggat ggctatttct tccctcgggc cgcgtcctgg
     1681 atgttctcgc cagccttctc gatgctgcgg ccggccttgt cggcggcgtt gtcgaccttg
     1741 cggccggcct tttccgcagg gccttcatgg cacgcgacga gcgcgagcag tgcgaacaag
     1801 gtaatgagcg ttttcattcc gcgagcgtag tcgggactgc tacagcacgc tatggcccgt
     1861 tcggggtacg taacttgctg ctcatgtcat cggatttttt cgcaaacgcg cgacacggaa
     1921 aaggagaccc gatgatttca cgcattgcaa ttactgccgc ccttgccacg ctgggcggcg
     1981 cagccgtggc gcaaacggca cagtgggaca gcggctatgt cctcgacacc aaccggcagg
     2041 cggcgcgcgg cgcgttcaac cagtgcgtgc gcaccgggta ctggaatgag tcgcttgccg
     2101 ccgagccctg cgacgccacg ccgcgcgcgg ggctggcccc ggtgccggtg gcacaggccg
     2161 cgcccgcgcc cgcgccggtg gtggccgcac cgctcgccgc gccgcgcccg gtgctggaga
     2221 aggtgacgct gtcgagcgac gtgctgttcg agttcggcaa ggcgaccctg cgcgactcgg
     2281 gcaagcagaa gctcgacgag ctggccggcc gcctcggcgg agccaacgtc gaggaggtag
     2341 tggccgttgg gcatgccgac cgcatcgaca gcgaagcgcg caaccagcgc ctctcggagc
     2401 agcgcgcgga ggcggtcaag gt
//
LOCUS       DASYJA010000463         7161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2248642, whole genome shotgun sequence.
ACCESSION   DASYJA010000463 DASYJA010000000
VERSION     DASYJA010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7161
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2248642"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2633)
                     /gene="purL"
                     /locus_tag="VGO02_10065"
                     /gene_synonym="purI"
     CDS             complement(<1..2633)
                     /gene="purL"
                     /locus_tag="VGO02_10065"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEV7478068.1"
                     /translation="MPHVLQLRGPRALSEFRLAKLAASLHKLDPGVRAVAAEFRHFVE
                     SEEQLAAAERALLERLLAYGPIPEKAAGDLFLVVPRVGTISPWSSKATDIARNCGLNV
                     RRIERGTAFHVQGASSDVSSLLHDRMTEHVFRSFEEAERLFEHVPPRPLQSVPVGALR
                     EANRRLGLALAADEIEYLEKAYRALGRDPTDAELTMFAQANSEHCRHKIFNADWIVDG
                     QRQDRSLFAMIRHTHAANPQGTVLAYADNSAILEGRVARRFFPDEKFTYQGKSELTHT
                     VIKCETHNHPSAISPFPGAATGSGGEIRDEGATGRGAKPKAGLVGFSVSHLRIPGALQ
                     PWEATDPGKPAHVASALEIMLDGPIGAASFNNEFGRPNLAGYFRTFESGNRGYHKPIM
                     LAGGLGSLSAVQSSKAPLPAGTLLVQLGGPGMLIGMGGGAASSMGAGANLAELDFDSV
                     QRGNAEIERRAQEVIDRCWQRGAANPILSIHDVGAGGLSNAVPEIVHAAGRGAHLNLR
                     AIPSEDSGMSPREIWCNEAQERYVLAIAPQNLSLFKSICERERCPFAVLGETTPDGKL
                     VVDDPLLGKRPIDIDLAVILGKPPKMLRDVTHVSSKPAAFSLERITLESAIHRVLRHP
                     AVADKTFLVAIGDRTVGGLCSRDPFVGPWQVPVADCAVTLADYEGYAGEAFAIGERTP
                     LALIDAPASGRMAVGEAITNIAAARIGSLKQIKLSANWMAAAGYPGEDAALYDTVKAV
                     AMDLCPALGLSIPVGKDSLSMRTAWQGKEIVAPLSLIVSAFASAEDARATLTPRLRTD
                     AGDSELILVDLGRGKNRLGGSILAQVHSQMGGECPDLDDPELLRGFFSAVQDLSEKKL
                     ILAYHDRSDGGLLTTVCEMAFA"
     gene            complement(2633..5578)
                     /locus_tag="VGO02_10070"
     CDS             complement(2633..5578)
                     /locus_tag="VGO02_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HEV7478069.1"
                     /translation="MNPTAIFSKSGKGVQEASGKTSQLSRPDRMVLSAIDGRATLADV
                     AGKVGKQFDNEFRSLINKLDKDGFIREVSPGAAGPTGKSAAAAPAKPASKPTAPMDIG
                     SDLDFTAAMAVPKKGAAAAPPPQTVARAPAPPPKPTVAVPPKPPPPSAAEQVKQAQIA
                     KEQQSAFAKAREEAERKQQEDRDRVKSEAEAKVRAETESRLRAQAEDKAKADAEAKAK
                     KAEADAATARSAAIRAAAEAKVKAEAEAKRAREEAERAKEEAERARREAEEKAERARK
                     EAEEKAERARREAEEKARREQEELKRKLEEERRAREEAERKTKEEAERARRELEEERR
                     KLEAERKAREEEAEQQSRRQRAEEEERERAEKEAARKREADEQSFEKLMAAQAAPPAA
                     PAPAAPPPPPKAKAATGDAFADSLLADLDSFTQRDDDERKAKEEAERKAKEEIARRKE
                     EEAEAQEREAAEVRQRMETERRRKEEEAQRVRDEEERRVRKQREEQEQAEREAEERRR
                     KVAAATGAAAAGGDDIGITDDDLELDEVKREAALLDKKKDRKEEREKEREKERAQKKE
                     REREAKSKKEKEREEKESKKSAAAYRPRRKSNFGRNAALGLFLLLIIGAGVVHVMPVS
                     TSEYERAATEALGLPVKIASGRFSMIGGVQYKFEGVSIGDLSVPLVRAYPEIGSVFGD
                     RKVFTRVELEGMSLPQEVLGDALFAKAKGDGLSVSQVTLKNLKLSGPLPLPPLDADVQ
                     LDSGGGMRTATLRGPEGLVAKLTAKEQAIEFDVNTAQLTLPFAQGITLVQFAMKGNAT
                     RQGMKISQWDGLLFSGHVTGTANLRWGNTWSIDGVMTARGVNAAVFAPALMSNGRAEG
                     TSRFSMSGVEPAKLGSSGRLEGSFTVSKGVLGSFDLSRALQTGGRQVTGQTQFAEMSG
                     QGTYDRGAVSLRNINLGAGALNAGASADISESGALSGRIVADVKTPTQNLRSTLILGG
                     TVQEPQVRN"
     gene            complement(5601..>7161)
                     /locus_tag="VGO02_10075"
     CDS             complement(5601..>7161)
                     /locus_tag="VGO02_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEV7478070.1"
                     /translation="KSKASAIGTSVDPVMLEIFNNLYMSIAEQMGLRLQNTAYSVNIK
                     ERLDFSCALFDAEGNLIANAPHMPVHLGSMGESIKTVIRLNAGKMKPGDVYVLNAPYN
                     GGTHLPDVTVITPVFSSLNEILFYVGSRGHHADIGGITPGSMPPGSKVVEEEGVLIDN
                     FLLVEQGRFREAEAVALLTSGKHPVRNVQQNIADLRAQIAANEKGVQELRRMVEHSGL
                     DVVRAYMRHVQDNAEESVRRVIEVLKDGAFELPLDNGAVIKVQIQINKQERKAKIDFS
                     GTSAQLPNNFNAPAAVSMAAVLYVFRTLVDDEIPLNAGCLKPLEVIIPEGCMLRPRYP
                     AAVVAGNVETSQCITDALYGALGVLAASQGTMNNFTFGNARHQYYETIAGGSGAGPDF
                     DGADVVQTHMTNSRLTDPEVLEWRFPVLLEGFEIRPGSGGRGRHRGGNGAVRRVRFLE
                     AMTAAILSGHRLVRPHGMAGGEPGRPGRNYVLRADGRRVVLGPFDQAEMAPGDVFVVE
                     TPGGGGYGLAD"
BASE COUNT          924 a         2523 c         2398 g         1316 t
ORIGIN      
        1 gcgaacgcca tctcgcacac cgtcgtcaac aggccgccgt ccgagcgatc gtggtaggca
       61 agaatcagct tcttttcaga aagatcttga acagcagaga aaaacccgcg caacagctcc
      121 ggatcatcca ggtcggggca ctcgccgccc atctgcgaat gcacctgcgc gaggatcgag
      181 ccgccgaggc ggttcttccc gcgccccagg tcgaccagga tcagctcgct gtcgccggcg
      241 tcggtgcgca ggcgcggcgt cagcgtcgcg cgcgcgtcct cggccgacgc gaacgccgag
      301 acgatcagcg aaagcggcgc cacgatctcc ttgccctgcc acgcggtgcg catcgacagc
      361 gaatccttgc ccaccgggat cgacaggccg agggcgggac acaggtccat ggccaccgcc
      421 ttcaccgtgt cgtagagcgc ggcgtcttcg ccgggatagc cggctgcggc catccagttc
      481 gcggaaagtt ttatttgctt caatgaacct attcttgccg cggcgatgtt ggtgatcgcc
      541 tcgcctaccg ccatgcgccc cgaggccggc gcgtcgatca gggcgagcgg cgtgcgctcg
      601 ccgatggcga aagcctcccc ggcgtagcct tcgtagtcgg cgagggtcac cgcgcagtcg
      661 gccaccggca cctgccaggg accgacgaac gggtcgcgcg agcacaggcc gccgaccgtg
      721 cggtcgccga tggcgacgag gaaggtcttg tcggccaccg ccgggtggcg caggacgcga
      781 tggatcgcgc tctccagggt gattctttcc agcgagaagg cagccggctt cgaagaaacg
      841 tgcgtcacgt cccgcagcat cttcggcggc ttgccgagga tcaccgcgag gtcgatgtcg
      901 atcggccgct tgccgagcag cggatcgtcg accaccagct tgccgtcggg cgtggtctcg
      961 cctagaaccg cgaacggaca tctttctcgt tcacaaatgc ttttgaacaa cgaaagattc
     1021 tgcggtgcga tcgcaaggac atagcgttcc tgcgcctcgt tgcaccagat ctcgcgcggc
     1081 gacatgccgc tgtcctcgga agggatggcc ctcagattga ggtgcgcgcc gcgccccgcc
     1141 gcgtgcacga tctcgggcac ggcattggac aggccgcccg cgccgacatc gtggatggac
     1201 aggatcgggt tcgccgcgcc ccgctgccag cagcggtcga tgacttcctg ggcgcggcgc
     1261 tcgatctctg cgttgccgcg ctgcaccgag tcgaagtcga gctcggcgag gttggcgccg
     1321 gcgcccatcg aggaggcggc gccgccgccc atgccgatca gcatgcccgg tccgccgagc
     1381 tgcacgagca gcgtgcccgc cggcagcggc gccttcgacg attgcaccgc gctgagcgag
     1441 ccgagcccgc ccgccagcat gatcggcttg tgatagccgc ggttcccgct ctcgaaggtc
     1501 ctgaagtagc cggccaggtt cggccggccg aactcgttgt tgaaggacgc cgcgccgatc
     1561 ggcccgtcga gcatgatctc gagcgccgag gcgacgtgcg ccggcttgcc gggatcggtc
     1621 gcctcccagg gctgcagcgc tcccgggatg cgcaggtggg acaccgagaa gccgaccagc
     1681 ccggccttcg gcttggcgcc gcggccggtg gccccctcgt cgcggatctc gccgcccgag
     1741 ccggtggcgg cgccggggaa cggcgagatg gcgctcgggt ggttgtgcgt ctcgcacttg
     1801 atgacggtgt gggtcagctc gctttttccc tggtaggtga atttttcatc cgggaagaaa
     1861 cgtcgcgcta cgcggccttc gaggatcgcc gagttgtccg cataggcgag gaccgtgccc
     1921 tgcggattgg cggcgtgcgt gtggcggatc atggcgaaga gcgagcggtc ctgcctctgg
     1981 ccgtcgacga tccagtcggc gttgaagatc ttgtgccggc agtgctccga gttcgcctgc
     2041 gcgaacatgg tcagctcggc gtcggtcggg tcgcggccca gcgcgcggta ggccttctcc
     2101 aggtattcga tctcgtccgc ggcgagcgcc aggccgagcc gccggttcgc ctcgcgcagc
     2161 gcgccgaccg gcaccgactg cagcggcctg ggcggcacgt gctcgaagag cctttcggct
     2221 tcttcgaatg agcggaagac gtgctcggtc atccggtcgt gcaacaggga agagacatcg
     2281 gaagaagcgc cttgcacatg gaaggcggtt cctctttcga tccttcgtac attcaagcca
     2341 cagttgcggg cgatgtcggt cgccttggac gaccacggcg aaatggtgcc gacgcgcggc
     2401 acgacgagga acaggtctcc cgccgccttc tcgggaatcg ggccgtaggc gagcagccgc
     2461 tcgagaagcg cgcgctcggc ggcggccaac tgctcttcgc tttccacgaa gtggcggaac
     2521 tcggcggcga ccgcgcgcac gcccggatcg agtttgtgca gcgaggcggc cagcttggcg
     2581 aggcggaact ccgacagcgc gcgcgggccg cggagctgga ggacgtgggg cactagttcc
     2641 tgacctgagg ctcctgaacg gttcccccta ggatcagcgt ggagcgcagg ttctgcgtcg
     2701 gggtcttgac gtcggcgacg atgcgccccg aaagcgcgcc gctttcgctg atgtcggcgc
     2761 tggcgccggc gttgagcgcg ccggcgccga ggttgatgtt gcgcaggctg accgcgccgc
     2821 ggtcgtaggt tccctggccg ctcatctcgg cgaactgcgt ctggccggtg acctgccggc
     2881 cgccggtctg cagggcgcgc gacaggtcga agctgcccag cacgcccttc gatacggtga
     2941 agctgccctc caggcgcccg ctcgagccga gcttggccgg ctcgacgccg ctcatcgaga
     3001 agcggctggt gccttcggcg cggccgttcg acatgagcgc gggggcgaag acggcagcgt
     3061 tgacgccacg cgccgtcatc acgccgtcga tgctccaggt gttcccccag cgcaggttgg
     3121 cggtgccggt gacgtggccg ctgaacagca gcccgtccca ctggctgatc ttcatgccct
     3181 gccgggtggc gttgcccttc atcgcgaact gcaccagcgt gatgccttgc gcgaagggca
     3241 gcgtgagctg cgcggtgttg acgtcgaact cgatcgcctg ctccttggcg gtgagcttcg
     3301 ccaccagtcc ctcggggccg cgcagcgtgg cggtgcgcat gccgccgccg ctgtcaagct
     3361 gcacgtcggc gtcgagcggc ggcaacggca gcgggccgct cagcttgagg tttttcagcg
     3421 tcacctgcga gacgctcagg ccgtcgccct tcgccttggc gaacagggcg tcgccgagca
     3481 cctcctgcgg caggctcatg ccctcgagct caacgcgggt gaagactttc ctgtcgccga
     3541 acaccgagcc gatctcggga taggcgcgca ccagggggac gctcaggtcg ccgatgctca
     3601 cgccctcgaa cttgtattgc accccgccga tcatggagaa gcggccggag gcgatcttca
     3661 ccggcaggcc gagcgcctcg gtcgccgctc tctcgtactc gctggtggag accggcatca
     3721 cgtgcaccac gccggcgccg atgatcagca gcaggaacag cccgagcgcg gcgttgcggc
     3781 caaagttcga cttgcggcgg gggcgatacg ccgccgcgga cttcttcgac tctttctcct
     3841 cgcgctcctt ttccttcttg ctcttggctt cgcgctcgcg ctccttcttc tgcgcccgct
     3901 ccttctcacg ctccttctcg cgctcctcct tgcggtcctt cttcttgtcg agcagcgccg
     3961 cctcgcgctt gacctcgtcc agctcgaggt cgtcgtcggt gatgccgatg tcgtcgccgc
     4021 cggccgctgc ggcgcccgtc gcggcggcta ccttgcgccg gcgctcctcg gcttcccgct
     4081 cggcctgctc ctgctcctcg cgctgcttgc gcacccggcg ctcttcctcg tcccgcacgc
     4141 gttgcgcttc ttcttccttg cggcggcgct cggtttccat gcgttgccgc acctccgccg
     4201 cctcgcgctc ctgggcttcg gcctcctcct ctttgcggcg cgcgatttcc tctttcgcct
     4261 tgcgctccgc ctcttccttg gccttgcgct catcgtcgtc gcgctgcgtg aacgagtcga
     4321 gatcggcgag cagcgaatcg gcgaacgcgt cgccggtcgc ggcctttgcc ttcggcgggg
     4381 gcggcggcgc ggccggggct ggcgcggcgg gcggggccgc ctgcgctgcc atgagcttct
     4441 cgaacgactg ctcgtccgcc tcgcgcttgc gcgccgcctc cttctccgcg cgctcgcgct
     4501 cttcctcctc cgcgcgctgt ctgcggctct gctgttccgc ttcctcctcg cgcgccttgc
     4561 gctccgcctc cagcttgcgg cgctcctcct cgagctcgcg gcgcgcgcgc tcggcctctt
     4621 ccttcgtctt gcgctcggct tcctcgcgcg ccctgcgctc ctcctccagc ttgcgcttga
     4681 gctcctcctg ctcgcgccgc gccttctcct cggcttcgcg gcgcgcgcgc tccgcctttt
     4741 cctcggcctc cttgcgcgcg cgctccgcct tctcctcggc ttcccggcgc gcgcgctcgg
     4801 cctcttcctt ggcgcgctcg gcctcttccc tggcgcgctt cgcctcggcc tcggccttga
     4861 ccttcgcttc ggcggcggcg cggatcgcgg ccgagcgcgc cgtggcggcg tcggcctccg
     4921 ccttctttgc cttcgcttcg gcgtcggcct tggccttgtc ttcggcctgc gcccggagcc
     4981 tggactcggt ctcggcgcgc accttcgcct cggcctcgga cttgacgcgg tcgcggtcct
     5041 cctgctgctt gcgctcggct tcctcgcgcg ccttggcgaa ggccgactgc tgctccttgg
     5101 cgatctgcgc ctgcttgacc tgctccgcgg ccgacggcgg cggcggcttc ggcggtacgg
     5161 cgaccgtcgg cttgggcggc ggcgcggggg cgcgcgccac cgtctgcggc ggcggggcgg
     5221 ctgcggcgcc cttcttcggc accgccatgg cggcggtgaa atccaggtcc gacccaatgt
     5281 ccatcggcgc ggtcggcttg ctggcgggct tggccggggc ggccgccgcc gacttgccgg
     5341 taggcccggc ggcgcccggc gacacctcgc ggatgaagcc gtccttgtcg agcttgttga
     5401 tgagcgacct gaactcgttg tcgaactgct tgccgacctt tcccgccacg tcggcaagcg
     5461 tggcgcggcc gtcgatcgcg gagagcacca tgcggtccgg gcgggacagc tggctggtct
     5521 tgccggacgc ttcctgcacg cccttgccgg acttggagaa gatggcggtg ggattcattc
     5581 tttgcgcgct attttatcgt ctagtcggcg agcccgtagc ccccgccgcc gggggtctcg
     5641 accacgaaca cgtcgccggg cgccatctcg gcctggtcga acggccccaa tacgacgcgg
     5701 cggccgtcag cgcgcagcac gtagttgcgc ccgggccggc ccggctcccc gccggccatg
     5761 ccgtgcggcc gcaccaggcg gtggccggag aggatcgccg cggtcatcgc ctcgaggaag
     5821 cgcacgcggc gcaccgcgcc gttgccgccg cggtgccggc cgcgcccgcc ggagcccggg
     5881 cggatctcga agccctcgag cagcaccggg aagcgccatt ccagcacttc cgggtcggtc
     5941 agccgcgaat tggtcatgtg ggtctgcacc acgtcggcgc catcgaaatc gggccccgcg
     6001 ccggagccgc cggcgatggt ctcgtagtac tggtgacgcg cattgccgaa ggtgaagttg
     6061 ttcattgtgc cctgcgaggc ggcgagcacc ccaagggcgc cgtacagggc gtcggtaata
     6121 cattgtgagg tttcgacgtt ccccgccacc acggccgccg ggtagcgcgg ccgcagcatg
     6181 cagccttccg ggatgatcac ctcgaggggc ttcaggcagc cggcgttgag cgggatctcg
     6241 tcgtcgacca gggtgcggaa gacgtagagc acggccgcca tggacaccgc ggccggggcg
     6301 ttgaagttgt tcggcagctg ggcggatgtc cccgagaagt cgattttcgc ttttctttcc
     6361 tgtttgttga tctggatttg caccttgatg acggcgccat tatcgagagg cagctcgaaa
     6421 gcgccgtcct tcagcacctc gatcacccgc cgcaccgact cctcggcgtt gtcctggaca
     6481 tggcgcatgt aggcgcgcac cacatcgagg ccggaatgct cgaccatgcg gcgcagctcc
     6541 tgcacgccct tctcgttggc ggcgatctgc gcgcgcaggt cggcgatgtt ctgctgcacg
     6601 ttcctcaccg ggtgcttgcc cgacgtcagc agcgcgacag cttcggcctc tcgaaagcgc
     6661 ccctgctcca ccagcaggaa gttgtcgatc agcacccctt cctcctccac cactttcgag
     6721 cccggcggca tggagcccgg ggtgatgcct cctatgtctg cgtgatgccc cctcgaaccg
     6781 acataaaaga gaatttcatt caaggaggag aagacaggcg ttatgaccgt cacatccggc
     6841 agatgggttc ccccgttgta gggcgcgttg aggacgtaga cgtcccccgg cttcatcttt
     6901 cccgcgttga gccggatcac ggtcttgatc gactcgccca tcgagcccag gtgcaccggc
     6961 atgtgcggcg cgttcgcgat caggttgcct tcggcgtcga acagcgcgca ggagaagtcg
     7021 agccgctcct tgatgttcac cgagtaggcg gtgttctgca ggcgcagccc catctgctcg
     7081 gcgatcgaca tgtagaggtt gttgaagatc tccagcatga ccgggtcgac cgaagtgccg
     7141 attgcgctcg ccttactttt t
//
LOCUS       DASYJA010000464         3034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1612217, whole genome shotgun sequence.
ACCESSION   DASYJA010000464 DASYJA010000000
VERSION     DASYJA010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612217"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VGO02_10080"
     CDS             complement(<1..252)
                     /locus_tag="VGO02_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV7478071.1"
                     /translation="MKYRTLGRSGVQVSQLCYGTMSFGGDADEAASAEMFKAVRELGV
                     NFFDTADQYSKGKSEEILGKLMRGSRDDLVVATKCFNASG"
     gene            345..>3034
                     /locus_tag="VGO02_10085"
     CDS             345..>3034
                     /locus_tag="VGO02_10085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HEV7478072.1"
                     /translation="MPKDFNFGLVFLSYFVAVTASHVTLLLAARVRDPHSVNWKLWVV
                     CGGLAMGLGIWSMHFVATLALRLPIQVLYDLRLTALSWIFAIVACGAAFIVLRRLTGR
                     HREFLLPGALIGIGIASMHYTGDASMRLSPGIQYDPVLFVASILVAMGAATAALWIAF
                     HLAKHRSVSANFGAALVMGAAVTGMHFTAVAAANIPADALCLASGPRLDDANMAYTVG
                     GTTFLILLATMLLSLYDARMSSAIALGVEKLRLANEQLEERVRERTQTLAREEARKDA
                     ILRTALDCIISMDAKGRIVEWNPAAEDTFGYPREEAIGRSLAEVIIPERLRQGHKAGL
                     ALYLATGEERIIGKRVELTATRRSGEEFPVEVAITVVRTDEGPLFTAHLRDITERKRA
                     EQELRESRDAARAGSEAKSTFVATMSHEIRTPMNAVIGMLELLGYSKLNPDQREMLST
                     ARESSRALLAVINDVLDFSKIEVGKLELHAEPCSVAQLVDGVIATFEHAASGKGILLA
                     RRIDPALWPAHRVDPARLRQILANLVSNAIKFTPSGTINLDVRMLASTQTHQTLCFSV
                     QDTGIGMSEETQKRLFEAFQQGESDTSRRFGGTGLGLTISRQLTELMGGEIRVTSAKD
                     LGTRVAVTLSAEQADPGAIRADPALGGRAAPMAVRAPAGTKVLIVDDHPLNLAMLKRQ
                     LKVLGLEADTAGSGPEALAKWRREAHRLVITDLQMPEMDGYALARAIRAEQLSGADSK
                     PTVVAYTANTQSEALERCTMAGMDDYLTKPTELGTLREKVTYWLGKPLVLARPRGRPR
                     AAGGDKLLDRARIEELAGGPEGIAEVLSGLEAAVRGDIAELQSALARGDLSAVRGAAH
                     RIKGAALSIGSERLAATAARLEQATRDPAEALLATEDLRRG"
BASE COUNT          468 a         1050 c         1060 g          456 t
ORIGIN      
        1 cccggaggcg ttgaagcact tggtggcgac gaccaagtcg tcgcggctgc cgcgcatcag
       61 cttgccgagg atctcctcgg acttgccctt gctgtactgg tcggcggtat cgaagaagtt
      121 gacgccgagc tcgcgcaccg ccttgaacat ctcggccgag gcggcctcgt cggcatcgcc
      181 gccgaaggac atcgtgccgt agcacagctg cgagacctgg acgccgctgc ggcctagcgt
      241 gcggtatttc acgcgttgca gtctatcagg ggtgttgaca cccttttgca gtcgattcgc
      301 gcacgcggct cacctgctgt gctattagac ctgcgggagg cggtatgcct aaggatttca
      361 atttcggact ggtcttcctg tcctatttcg tggcggtgac cgcatcgcac gtcaccctgc
      421 tgcttgcggc gcgcgtgcgc gacccgcact cggtcaactg gaagctgtgg gtggtatgcg
      481 gcggcctggc gatgggcctg ggcatctggt cgatgcactt cgtcgccacg ctcgcgctgc
      541 gcctgccgat ccaggtgctc tacgacctgc ggctcaccgc tttgtcctgg atattcgcca
      601 tcgtcgcctg cggcgcggcg ttcatcgtgc tgcgccggct caccggccgc caccgcgagt
      661 tcctgctgcc cggcgccctg atcggcatag gcatcgcgtc gatgcactac accggcgatg
      721 cctcgatgcg cctgagcccg gggatccagt acgacccggt cctgttcgtg gcgtcgatcc
      781 tggtggcgat gggcgcggcc accgccgccc tgtggatcgc gttccacttg gcgaagcatc
      841 gctcggtgag cgcgaatttc ggcgccgcgc tggtgatggg cgcggcagtg accggcatgc
      901 acttcaccgc cgtggcggcg gcgaacatcc cggccgacgc cctctgcctg gcgagcggac
      961 cgcgcctgga cgacgccaac atggcctaca ccgtgggcgg caccacgttc ctgatcctgc
     1021 tcgcgaccat gctgctttcg ctgtacgacg cgcgcatgtc gtcggcgatc gcgctcggcg
     1081 tcgagaagct gcgcctggcg aacgagcagc tcgaagagcg cgtgcgcgag cgcacgcaga
     1141 cgctggcgcg ggaagaggcg cgcaaggatg cgatcctgcg cacggcgctc gactgcatca
     1201 tctccatgga tgcgaagggc cgcatcgtcg agtggaaccc cgcggcggag gataccttcg
     1261 gctacccgcg cgaggaggcg atcggccgct cgctcgcaga agtcatcatt cccgagcggc
     1321 tgcggcaggg gcacaaggcc gggctggcgc tgtacctcgc cacgggcgag gagcggatca
     1381 tcggaaaacg cgtcgagctg accgccacgc ggcgcagcgg cgaggagttc ccggtcgagg
     1441 tggcgatcac ggtggtgcgc accgatgagg gcccgctgtt caccgcgcac ctgcgcgaca
     1501 tcaccgagcg caagcgcgcc gagcaggagc tgcgcgagtc gcgcgacgcg gcgcgcgccg
     1561 gctcggaggc gaagagcacc tttgtcgcca ccatgagcca cgagatccgc acgccgatga
     1621 acgcggtgat cggcatgctc gagctgctgg ggtacagcaa gctcaacccc gaccagcgcg
     1681 agatgctgtc gaccgcgcgt gaatcgagcc gcgcgctgct cgccgtgatc aacgacgtgc
     1741 tggatttctc caagatcgag gtcggcaagc tcgagctgca cgccgagccc tgctcggtgg
     1801 cgcagctggt ggacggcgtg atcgcgactt tcgagcacgc cgcgagcggc aaggggatcc
     1861 tgctcgcgcg gcgcatcgac ccggcgctct ggccggcgca ccgggtcgat ccggcgcgcc
     1921 tgcgccagat cctcgccaac ctggtcagca acgccatcaa gttcaccccg tcgggcacca
     1981 tcaacctcga cgtgcgcatg ctcgcctcca cgcagacgca ccagacgctg tgcttcagcg
     2041 tgcaggacac cggcatcggc atgagcgaag agacgcagaa gcgcctgttc gaggccttcc
     2101 agcagggcga atccgacacc agccggcgct tcggcggtac cggcctgggg ctcaccatct
     2161 cgcgccagct caccgagctg atgggcggcg agatccgcgt caccagcgcc aaggacctcg
     2221 gcacgcgggt cgcggtgacg ctcagcgccg agcaggcgga ccccggcgcg atccgggcag
     2281 acccggcgct cggtgggcgt gccgctccga tggcggtgcg tgcgcccgcc ggcaccaagg
     2341 tgctgatcgt cgacgaccac ccgctcaatc tcgccatgct gaagcgccag ctgaaggtgc
     2401 tcgggctgga ggccgatacc gctggcagcg ggcccgaggc cctcgccaag tggcgccgcg
     2461 aggcgcaccg gctggtgatc accgacctgc agatgcccga aatggacggc tacgcgctcg
     2521 cgcgcgcgat ccgcgccgag cagctgtcgg gcgcggacag caagccgacc gtggtcgcct
     2581 ataccgccaa tacgcagagc gaggcgctcg agcgctgcac catggcgggc atggacgact
     2641 acctcaccaa gccgacggag ctcggcacgc tgcgcgagaa ggtcacctac tggctgggta
     2701 agccgctggt gcttgcgcgg ccgcgcggcc gcccgagagc cgctggcggc gacaagctgc
     2761 tcgaccgcgc gcgcatcgaa gagctggcgg gcgggccgga gggcatcgcc gaggtgctct
     2821 ccgggctgga ggccgcggtg cgcggcgaca tcgccgagct gcagtcggca ctggcgcgcg
     2881 gcgacctgtc cgccgtgcgc ggcgctgccc atcgcatcaa aggggcggcg ctttccatcg
     2941 gctcggagcg cctcgccgca acggccgcca ggctggaaca ggcgacgcgc gacccggcgg
     3001 aggcgctgct cgccaccgaa gacttgcggc gagg
//
LOCUS       DASYJA010000465         2564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1069788, whole genome shotgun sequence.
ACCESSION   DASYJA010000465 DASYJA010000000
VERSION     DASYJA010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069788"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="VGO02_10090"
     CDS             complement(<1..1131)
                     /locus_tag="VGO02_10090"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEV7478073.1"
                     /translation="MLKSGAEHLRQIRDGRVIYIGAEQVDDVTTHPAFRNAARTVAAI
                     YDAKAADPSLAFEEKGERYSNYFLRAKTREDLGKRKAIHEVIARMSHGLLGRSPDHVS
                     SFVTGMAMNPSVFGKFEKNLLDYYEHMRREDVYAVYAVIPPQAARNPEFYEKQNLPIP
                     TLRVVREDDDGVVISGMKMLATGAVFANEIWIGNLIPLAPNQLAESITCAIAVNAPGV
                     ALWSRKPFERHAQSEFDSPLAWRFDESDSMVMCDNVKVPWERVFVHMDAVLARDIYIK
                     TPGHCYGNHQSNVRFHAKMRLIVGLASRIAQASGAHEIPAVREVLGRFAALEAGLGGM
                     IAGQLQDAEDWPAGWKTFNRRYMYAALNWCTESHSQIIDALRE"
     gene            complement(1112..1498)
                     /locus_tag="VGO02_10095"
     CDS             complement(1112..1498)
                     /locus_tag="VGO02_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEV7478074.1"
                     /translation="MKAKQHLEFVKVDLNAGWERPAGYPSGFWQKVLASDLDEKAKRG
                     SRTRLLKIDAGAFSTEPFVHDHWEEVYVVQGDLVVGNDRHGKGGQQCFAPTYACRPPG
                     VHHGPFTSRGGCVLHEIHYYDAQERR"
     gene            complement(1495..2028)
                     /locus_tag="VGO02_10100"
     CDS             complement(1495..2028)
                     /locus_tag="VGO02_10100"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV7478075.1"
                     /translation="MPRARKRNAAPLTVSRPALLVDGSDAEFRAFIHDLIAYGHRLDA
                     CRGAFAAIAGVSAAQYEILMLVSRTDGMAVGEVAARLHRSGAFITIESGKLVERGVLQ
                     KAADPADGRRVLLQATRKSREILQRLAPYQVRINDVLFGHLGARHFREVRALLREQLA
                     SGDRAVALLDVLLQEAA"
     gene            2132..>2564
                     /locus_tag="VGO02_10105"
     CDS             2132..>2564
                     /locus_tag="VGO02_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEV7478076.1"
                     /translation="MIRLFAAATPDARKVSVMLEETGLAYEIERREQPPAIEDDEVPL
                     GLAEPAAILIYLAERTGRFFPVERRYRYPALQWLVFQERDGEKLCAALDKRLGEAEFL
                     AGVYSIADIAAYPWVVRHAEYGLDLEAYPAVKRWADALGARP"
BASE COUNT          387 a          890 c          847 g          440 t
ORIGIN      
        1 ctcgcgcagc gcgtcgatga tctgcgaatg gctctcggtg caccagttga gcgcggcgta
       61 catgtagcgg cggttgaagg tcttccagcc cgccggccag tcctcggcgt cctgcagctg
      121 gccggcgatc atgccgccga gccccgcctc gagcgcggcg aagcgcccca gcacctcgcg
      181 caccgccggg atctcgtgcg cgccggaggc ctgcgcgatg cggctcgcca ggccgacgat
      241 caggcgcatc ttggcgtgga agcgcacgtt ggactggtgg ttgccgtagc aatggccggg
      301 cgtcttgata tagatgtcgc gcgcgagcac cgcgtccatg tgcacgaaca cccgctccca
      361 cggcaccttg acgttgtcgc acatcaccat cgagtcgctc tcgtcgaagc gccaggcgag
      421 cgggctgtcg aactcgctct gcgcgtggcg ctcgaagggc ttgcgcgacc acagcgccac
      481 ccccggcgcg ttcaccgcga tggcgcaggt gatcgactcg gccagctggt tcggcgccag
      541 cgggatcagg ttgccgatcc agatctcgtt ggcgaacacc gcgccggtgg cgagcatctt
      601 catgcccgag atcaccaccc cgtcgtcgtc ctcgcgcacc acgcgcagcg tcgggatcgg
      661 caggttctgc ttctcgtaga actccgggtt gcgcgccgcc tgcggcggga tcacggcgta
      721 gacggcgtag acgtcctcgc gccgcatgtg ctcgtagtaa tcgagcagat tcttctcgaa
      781 cttgccaaac accgagggat tcatcgccat gccggtgacg aagctggaca catggtcagg
      841 cgagcggccc agcaacccgt ggctcatcct cgcgatcact tcgtgaatcg cctttcgctt
      901 gccgaggtct tctctggtct tcgccctgag aaagtagttg ctgtagcgtt cccccttctc
      961 ctcgaaagcg agagatgggt cggcagcctt cgcgtcgtag atcgcggcca cagtgcgcgc
     1021 ggcgttgcgg aacgccgggt gcgtggtgac gtcgtcgacc tgctccgcgc cgatgtagat
     1081 cacgcggccg tcgcggatct gcctcaggtg ctcagcgccg ctcttgagca tcgtagtagt
     1141 ggatctcgtg gaggacgcag ccgccgcgcg aagtgaaggg cccgtggtgc acgccgggag
     1201 ggcggcaggc ataggtgggc gcaaagcatt gctgcccgcc tttgccgtgc cggtcgttgc
     1261 cgaccaccag gtcgccctgt accacataca cctcttccca atgatcgtgt acgaagggct
     1321 cggtcgagaa ggcgccggcg tcgatcttca gcagccgggt cctgcttccc ctcttcgctt
     1381 tttcgtcaag atccgaggcg aggactttct gccagaagcc ggacggatag cccgccgggc
     1441 gctcccagcc tgcattcagg tccaccttca cgaactcgag atgctgtttt gctttcatgc
     1501 ggcctcctgc agcagcacat ccagcagcgc caccgcgcgg tcgccagacg cgagctgctc
     1561 gcgcaacagc gcgcgcacct cgcggaagtg cctggcgccg aggtggccga acaggacgtc
     1621 gttgatgcgc acctggtagg gcgcgaggcg ctgcaggatc tcgcggctct tgcgcgtcgc
     1681 ctgcagcagc acgcggcgcc cgtcggccgg gtcggccgcc ttctgcagca cgccgcgctc
     1741 caccagcttg ccgctctcga tggtgatgaa ggcgccggag cgatgcagcc tcgccgccac
     1801 ctcgccgacc gccatgccgt cggtccggct gaccagcatc aggatttcgt actgcgcggc
     1861 ggagacgccg gcgatggcgg cgaaggcgcc gcggcaggcg tcgaggcggt ggccgtaggc
     1921 gatcaggtcg tggatgaagg cgcggaactc cgcgtcggag ccgtccacca gcagcgccgg
     1981 acgcgagacg gtaagaggcg ctgcgtttcg cttgcgagcc ctgggcatcg gccgcctccc
     2041 gtttgacgca aacgattctc gacccgacaa ttacctttgt caattagcta tctgcgcctc
     2101 cgatgcccct tctcgcgacc cgcctaccct tgtgatccga ctatttgccg cagccacgcc
     2161 cgacgcccgc aaggtttcgg tgatgctcga ggagaccggg ctcgcctacg agatcgagcg
     2221 gcgcgagcag ccgcccgcca tcgaggacga cgaggtgccg ctcgggctgg ccgagcccgc
     2281 cgcgatcctg atctatctcg ccgagcgcac cggccgcttc ttcccggtgg agcggcgcta
     2341 ccgctacccg gcgctgcagt ggctggtgtt ccaggaaagg gacggcgaga agctgtgcgc
     2401 cgcgctcgac aagcgcctgg gcgaagccga gttcctcgcc ggcgtgtact ccatcgccga
     2461 catcgcggcc tacccctggg tcgtgcgcca cgccgagtac ggcctggacc tcgaggccta
     2521 ccccgcggtc aagcgctggg ccgacgcgct cggcgcgcgg cccg
//
LOCUS       DASYJA010000466         3845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_525202, whole genome shotgun sequence.
ACCESSION   DASYJA010000466 DASYJA010000000
VERSION     DASYJA010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525202"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VGO02_10110"
     CDS             complement(<1..192)
                     /locus_tag="VGO02_10110"
                     /inference="COORDINATES: protein motif:HMM:NF016326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HEV7478077.1"
                     /translation="MKLHRAGASPVNTFTGYGEGYVLVNGRRHESSVIVLPEQIVPWD
                     VSEFSKITAADFEKLKTLDA"
     gene            207..1403
                     /gene="alaC"
                     /locus_tag="VGO02_10115"
     CDS             207..1403
                     /gene="alaC"
                     /locus_tag="VGO02_10115"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HEV7478078.1"
                     /translation="MREFSRIKRLPPYVFNITNELKMAARRRGEDVIDLSMGNPDGPT
                     PKHIVDKLVEASARQDTHGYSVSKGIPRLRKAICAWYGRRYGVELDPDSEAIVTIGSK
                     EGLAHLMLACLERGDTVLVPNPSYPIHIYGAIIAGADIRSVRMTPGVDFFDELERAIR
                     ELTPKPKMLILGFPSNPTAQCVDLNFFERIVALARQHDILVVHDLAYADICFDDYRAP
                     SIMQVPGARDIAVEFFTMSKSYNMAGWRIGFMVGNKDLVHALARIKSYHDYGSFTPIQ
                     VASIVALEGPQECVSQIRETYEKRRNVMVKALHDAGWMVEKPRASMYIWAKIPEFYAK
                     MGSIEFTKRLLEEAKIAVAPGIGFGDYGDDHVRIALIENEHRLRQAARGIRDMFRKDG
                     LLRGAA"
     gene            1400..1999
                     /locus_tag="VGO02_10120"
     CDS             1400..1999
                     /locus_tag="VGO02_10120"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEV7478079.1"
                     /translation="MIHPKPATLEGNGVRLEPLEAKHEEGLKAAAADGKLWELFFTSV
                     PEPDQVSTYISQALEKQKQGEMLPWAVRDLKTNAIVGSTRYHDIIAAADRVEIGYTWY
                     AQRCQRSHVNTACKLLLFSFAFEKNNCKVVGLRTDNFNFRSQRAIEALGAKKDGVIRH
                     HWPRRDGSVRDTVMYSVLREEWDGVRKHLELRLKKHEAH"
     gene            1986..3296
                     /locus_tag="VGO02_10125"
     CDS             1986..3296
                     /locus_tag="VGO02_10125"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEV7478080.1"
                     /translation="MKPINVGLLGIGTVGGGTWDVLKRNASEIQRRAGRAIRITRVAD
                     QAVQKARKITKGKAKIHADAFEVVSAPDIDIVVELIGGYTIAKQLVLEAIRHGKHVVT
                     ANKALLATHGNEIFKAAQKRGVMVAFEASVGGGIPIIKALREGLAANRIEWIAGIING
                     TSNFILSEMRDKGLAFGAALADAQKRGYAEADPTFDIEGVDAAHKLTILSALAFGIPM
                     QFRKAYVEGISKLTREDIGYAEQLGYRIKLLGITKKTDKGIELRVHPTLVPTRRLIAN
                     VEGVMNAILVKGDAVGPTLYYGAGAGSQPTASAVVADLVDVTRLITADPEHRVPHLAF
                     QPDQLSAVPILGIGQVETSYYLRMRVVDKPGVLADITRLLARSKISIDAMVQKEPAQG
                     ERQVDIVMLTHRALEKNVDEAIRRIERLPTVLGKIVRLRLEELL"
     gene            3293..3466
                     /locus_tag="VGO02_10130"
     CDS             3293..3466
                     /locus_tag="VGO02_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478081.1"
                     /translation="MRRLALAAALLVSLPLCAQDKVPAKKKKAAAAPKAAHQQASKEQ
                     IRKFNEMQKKQQK"
     gene            3463..>3845
                     /locus_tag="VGO02_10135"
     CDS             3463..>3845
                     /locus_tag="VGO02_10135"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741565.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV7478082.1"
                     /translation="MKYISTRGGGAPQKFSGILLEGLAPDGGLYVPESFPKADLAGWR
                     GLSYPDLACAILSQFMDDIPDLEKIVKATYTREIFGSDDITPLKTLEPGFHLLGLSNG
                     PTLAFKDIALQLLGRLLESVLEKKK"
BASE COUNT          771 a         1236 c         1212 g          626 t
ORIGIN      
        1 cgcgtccagc gttttcagct tttcgaagtc tgccgccgtt attttcgaga attcagaaac
       61 atcccacggc acgatctgct ccggcagtac gatcacgctc gattcgtgcc gccggccgtt
      121 caccagcacg tagccttcgc cgtagccggt gaaggtgttg acggggctcg cgccggcgcg
      181 gtgaagtttc acgctagaat tctacaatgc gtgagttttc aaggattaag cggcttccgc
      241 cgtacgtctt caacatcacc aacgagttga agatggcggc gcgccggcgc ggcgaggacg
      301 tcatcgacct gtcgatgggc aaccccgacg ggccgacgcc gaagcacatc gtcgacaagc
      361 tggtggaagc gagcgcgcgc caggacacgc acggctactc ggtgtcgaag ggcatcccgc
      421 gcctgcgcaa ggcgatctgc gcctggtacg gccggcgcta cggcgtcgag ctcgatccgg
      481 actcggaagc gatcgtcacc atcggctcga aggaaggtct cgcgcacctg atgctggcgt
      541 gtctcgagcg cggcgacacg gtgctggtgc ccaacccctc ctacccgatc cacatctacg
      601 gcgcgatcat cgccggcgcc gacatccgct cggtgcgcat gacgccgggg gtggatttct
      661 tcgatgagct cgagcgcgcc atccgcgagc tcacgcccaa gccgaagatg ctgatcctgg
      721 gattcccatc caatcccacg gctcaatgcg tcgatttgaa tttttttgag cgtatcgtgg
      781 cgcttgcgcg acagcacgac attctggtgg tgcacgacct cgcctacgcc gacatctgct
      841 tcgacgacta ccgcgcgcct tcgatcatgc aggtgccggg cgcccgcgac atcgcggtcg
      901 agttcttcac catgtcgaag agctacaaca tggccggctg gcgcatcggc ttcatggtcg
      961 gcaacaagga cctggtgcac gctctggcgc gcatcaagag ctaccacgac tacggcagct
     1021 tcacgccgat ccaggtagct tccatcgtgg cgctggaagg accacaagaa tgtgtttctc
     1081 aaataagaga aacatatgaa aagcgtcgca atgtcatggt gaaggcgctg cacgacgcgg
     1141 gctggatggt ggagaagccc agggcgtcga tgtacatctg ggccaagatc cccgagttct
     1201 acgccaagat gggctcgatc gagttcacca agcgcctgct cgaggaggcg aagatcgcgg
     1261 tggcgcccgg catcggcttc ggcgactacg gcgacgacca cgtgcgcatc gcgctgatcg
     1321 agaacgagca ccgcctgcgc caggcggcgc gcggcatccg cgacatgttc cgcaaggacg
     1381 gcctgctgcg gggcgcggct tgatccaccc caagccggcc acgctcgaag gcaacggcgt
     1441 ccggctcgaa cccctggaag cgaagcacga ggaaggcctg aaggctgctg ccgcggacgg
     1501 caagctgtgg gagctgttct tcacctcggt gcccgagccg gaccaggttt ctacctatat
     1561 ttctcaagct ttagagaagc agaagcaagg cgagatgctg ccctgggccg tgcgcgacct
     1621 gaagaccaac gccatcgtcg gcagcacgcg ataccacgac attatcgccg ctgccgaccg
     1681 cgtcgagatc ggctacacct ggtacgcgca gcgctgccag cgcagccacg tgaacacggc
     1741 gtgcaagctg ctgctctttt cttttgcttt tgaaaaaaac aattgcaaag tcgtcgggct
     1801 tcgcaccgac aacttcaact tccgctcgca gcgcgccatc gaggcgctcg gcgcgaagaa
     1861 ggacggcgtc atccgccacc actggccgcg gcgcgatggc agcgtgcgcg acacggtcat
     1921 gtacagcgtg ctgcgggagg agtgggacgg cgtgaggaag catctggagc tgcggctgaa
     1981 aaaacatgaa gcccattaac gtcggcttgc tgggcatcgg cacggtcggc ggcggcacct
     2041 gggacgtgct caagcgcaac gcgagcgaga tccagcgccg cgctggccgg gcgatccgta
     2101 ttacgcgagt ggccgaccag gcggtccaaa aagcaagaaa aatcacaaaa ggcaaagcga
     2161 agatccatgc cgatgctttc gaggtggtaa gcgcgcccga catcgacatc gtggtcgagc
     2221 tgatcggcgg ctacaccatc gccaagcagc tcgtgctgga ggcgatccgc cacggcaagc
     2281 acgtggtcac cgccaacaag gcgctgctcg ccacgcacgg caacgagatc ttcaaggcgg
     2341 cgcagaagcg cggcgtgatg gtcgccttcg aggcctcggt gggcggcggc atcccgatca
     2401 tcaaggcgct gcgcgaaggc ctcgccgcca accgcatcga gtggatcgcc ggcatcatca
     2461 acggtacgtc gaacttcatc ctctccgaga tgcgcgacaa ggggcttgcc ttcggcgccg
     2521 ccctggccga cgcgcagaag cgcggttacg ccgaggccga ccccaccttc gacatcgagg
     2581 gcgtcgacgc cgcgcacaag ctcaccatcc tgtcggcgct cgccttcggc attccgatgc
     2641 agttcaggaa ggcctacgtc gaaggcatct cgaagctgac gcgcgaggac atcggctacg
     2701 ccgagcagct tggctaccga attaaattat tgggaattac aaaaaagaca gataaaggaa
     2761 tcgagctcag ggtccacccc acgctcgtgc cgacgcgccg cctgatcgcc aacgtcgagg
     2821 gcgtgatgaa cgccatcctg gtgaagggcg acgccgtcgg cccgacgctc tactacggcg
     2881 ccggcgcggg cagccagccc accgcgagcg cggtggtggc cgacctggtc gacgtgacgc
     2941 gcctgatcac cgccgatccg gagcaccgcg tgccgcacct cgcgttccag cccgaccagc
     3001 tctccgcggt gcccattctg ggaatcggac aggtggaaac ctcgtactac ctgcgcatgc
     3061 gggtggtcga caagcccggc gtgctcgccg acatcacgcg gctgctggcg cgctcgaaga
     3121 tctcgatcga cgccatggtg cagaaggagc cggcccaggg cgagcggcag gtggacatcg
     3181 tcatgctgac ccaccgcgcg ctcgagaaga acgtcgacga ggcgatcagg cgcatcgagc
     3241 gcctgcccac ggtgctcggc aagatcgtgc gcctgcgcct ggaggaactg ctgtgaggcg
     3301 gctggcgctc gcggccgcgc tgctcgtcag cctgccgctt tgcgcccagg acaaggtgcc
     3361 cgccaaaaag aagaaggctg cggccgcgcc gaaggcggcg caccagcagg cctcgaagga
     3421 gcagatccgc aagttcaacg agatgcaaaa gaaacagcag aagtgaaata catcagtacc
     3481 cgcggcggcg gcgcgccgca gaagttctcc ggcatcctgc tcgaagggct cgcccccgac
     3541 ggcggcctgt acgtccccga gagcttcccc aaggcggacc tcgccggctg gcgggggctg
     3601 tcctacccgg atctcgcctg cgcgatcctt tcgcagttca tggacgacat tccggatctc
     3661 gagaagatcg taaaggcaac gtatacgagg gagatcttcg gcagcgacga catcacgccg
     3721 ctgaagaccc tcgagcccgg ctttcatctg ctcggcctgt cgaatgggcc aacccttgcg
     3781 ttcaaggata ttgccttgca gcttctaggg cggctgttgg aatccgttct cgaaaagaaa
     3841 aagca
//
LOCUS       DASYJA010000467         3152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2158377, whole genome shotgun sequence.
ACCESSION   DASYJA010000467 DASYJA010000000
VERSION     DASYJA010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2158377"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VGO02_10140"
     CDS             complement(<1..393)
                     /locus_tag="VGO02_10140"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV7478083.1"
                     /translation="MELAAGTVALIAITFFAAFVNGALGYGFSSLTVPVALLFHSNRI
                     LNPALVLVEVFINLYVLYINRSAIPAVWKRVLPILLGLAPGIALGSYLIASLHPAWLK
                     FGTYAFLLPLILVQAAGIRRPIKSEKLFG"
     gene            466..1407
                     /locus_tag="VGO02_10145"
     CDS             466..1407
                     /locus_tag="VGO02_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEV7478084.1"
                     /translation="MKLQQLRCLIEVVRRGLNVSEAAEALHTSQPGVSKQIRALEDEL
                     QVQVFARHGKRLVSVTEPGKAVIAIAERILAEAQNLRRAGEEFSNDQIGTLTVAATHT
                     QARYALPKAVAAFKRRYPKVELVLHQGNPTQICDQVLAGEADMGVATEMIANYPELVS
                     LPVYQWNRVVVVPPKHPLLKGPLTLEALAEHPIVTYDFAFANRSLVQKAFENRGLKPH
                     VVLSAQDSDVIKTYVELGLGVGILAKMAFDPKRDRELRAIDAGHLFESSTTRLGIKRG
                     AYLRRYAYEFIELFAPQLPRNIVERAVVGAEGSRYEL"
     gene            complement(1391..2581)
                     /gene="phaZ"
                     /locus_tag="VGO02_10150"
     CDS             complement(1391..2581)
                     /gene="phaZ"
                     /locus_tag="VGO02_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEV7478085.1"
                     /translation="MLYNLYEAQHTALTPYRYLAELIRGWYGNPFSPFHYLPLASRLA
                     AGSDLFLRVTGRYEKPEWGIDGLEVFQEKPFCKLIHFKQASAGKKKVLVVAPLSGHHA
                     TLLRDTVRSLLAEHDVWVTDWVDARMVPLSAGAFHLADYVRYVEEWIRLLNDPKRPDL
                     SVISVCQPTVPVLAAISLMAAREEPLPRAMVMMGGPIDARKSPTAVNNLARTKPHSWF
                     EQNVIQRVPNKYPGYMRRVYPGFLQHLGFVAMNPSRHLSAHWDYYGHLLQGDGDSAEA
                     HRRFYDEYNAVLDMPAEYYLDTVKTVFQDFALPKGRMFVRDELVRPQAIRDTALLTIE
                     GELDDISGNGQTEAAHALCLNIPRERREHFLAPGVGHYGIFSGRRWREMIFPKVSAFI
                     KAHT"
     gene            2737..>3152
                     /locus_tag="VGO02_10155"
     CDS             2737..>3152
                     /locus_tag="VGO02_10155"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEV7478086.1"
                     /translation="MSGISRPVEILRDAFGIPHIFAESIDDAHFALGYVHAQDRLWQM
                     EMNRRVAAGRLAEVLGPGALESDRFLRTLGVHRVARSNLEHYDAETRHVLDAYAAGVN
                     AFLAGRPVLPPEFWLARVSPEPWSPADSVGWTKMMA"
BASE COUNT          531 a         1039 c         1044 g          538 t
ORIGIN      
        1 gccgaacagc ttctccgact tgatcggccg ccggatgccc gccgcctgga cgaggatcag
       61 cggcaggagg aaggcgtagg tgccgaactt gagccacgcc ggatgcagcg aagcgatgag
      121 gtagctgccg agcgcgatgc cgggcgccag gccgagcagg atcggcagca cccgcttcca
      181 caccgccggg atcgccgagc ggttgatgta cagcacgtac aggttgatga acacctcgac
      241 cagcaccagc gccgggttga ggatgcggtt gctgtggaag agcagcgcga ccgggacggt
      301 cagcgaggag aagccgtagc cgagcgcccc gttgacgaac gcggcgaaga acgtgattgc
      361 gatgagcgct acggtacccg cggccagttc catccctgcg aatgctatag agcggcctta
      421 ttctttcaaa gactattgtg ttagtatctt attacttcag gatatatgaa gctccagcaa
      481 ctccgctgtc tgatcgaagt cgtccgccgg gggctgaacg tctcggaagc ggccgaggcg
      541 ctgcacactt cccagccggg cgtttcgaag cagatccgcg ccctcgagga cgagctgcag
      601 gtccaggtct tcgcgcggca cggcaagcgc ctggtttccg tgaccgagcc gggcaaggcg
      661 gtcatcgcca tcgccgagcg gatcctcgcc gaggcgcaga acctgcgccg ggccggggag
      721 gagttctcca acgaccagat tggcactttg acggtagcgg ccactcacac ccaggcccgc
      781 tacgcccttc ccaaggcggt ggccgcgttc aagcgccgct accccaaagt cgagctcgtc
      841 ctgcaccagg gcaacccgac gcagatctgc gaccaggtgc tcgccggcga ggccgacatg
      901 ggcgtggcca ccgagatgat cgccaactac cccgagctcg tctcgctgcc cgtctaccag
      961 tggaaccgcg tggtcgtggt gccgcccaag catccgctgc tcaaggggcc gctcacgctg
     1021 gaggcgctgg ccgagcaccc catcgtcacc tacgacttcg ccttcgccaa ccgctcgctg
     1081 gtgcaaaagg cgttcgagaa ccgcggcctc aagccgcacg tggtgctgag cgcgcaggac
     1141 tcggacgtca tcaagaccta cgtcgagctc gggctggggg tggggattct ggcgaagatg
     1201 gccttcgacc cgaagcgcga ccgcgagctg cgcgccatcg atgccggcca cctgttcgag
     1261 tcgagcacta cgcgcctggg catcaagcgc ggcgcctacc tgcggcgcta cgcctacgag
     1321 ttcatcgagc tgttcgcccc gcagctgccg cgcaacatcg tcgagcgcgc cgtggtcggc
     1381 gccgagggct ctaggtatga gctttgatga aggcgctgac cttcggaaaa atcatctcgc
     1441 gccagcggcg gcctgagaaa atgccgtagt ggccgacgcc gggggcgagg aagtgctcgc
     1501 gccgctcccg ggggatgttc aggcagagcg catgcgccgc ttccgtctgc ccgttgcccg
     1561 agatgtcgtc gagctcgccc tcgatggtga gcagcgcggt gtcgcggatc gcctgcgggc
     1621 gcaccagctc gtcgcgcacg aacatgcggc cctttggcag cgcgaaatcc tggaacaccg
     1681 tcttgaccgt gtcgaggtag tactcggccg gcatgtcgag caccgcgttg tattcgtcgt
     1741 agaagcgccg gtgcgcctcg gccgagtcgc cgtcgccctg cagcagatgc ccgtagtagt
     1801 cccaatgcgc gctcaggtgg cggcttggat tcatcgccac gaagcccagg tgctgcagga
     1861 acccggggta cacgcggcgc atgtagccgg gatatttgtt cggcacccgc tggatgacgt
     1921 tttgctcgaa ccacgaatgc ggcttggtgc gggcgaggtt gtttaccgcg gtggggctct
     1981 ttctcgcgtc gatcggcccg cccatcatga ccatggcgcg cggcagaggc tcttcgcgcg
     2041 cggccatcag cgagatcgcc gccagcaccg gcaccgtggg ctggcacacc gagatcacgc
     2101 tcaggtcggg acgcttcggg tcgttcagca ggcggatcca ctcttccaca tagcgcacgt
     2161 agtcggcgag gtggaacgct ccggcggaga gcggcaccat gcgcgcatcg acccaatcgg
     2221 tgacccacac gtcgtgctcg gcgaggagcg agcgcaccgt atcgcgcagc agcgtcgcgt
     2281 ggtgccccga gaggggcgcc accaccagga cttttttctt tccagcgctt gcctgcttga
     2341 aatggatcag cttgcagaac ggcttttcct ggaacacctc gaggccgtcg atgccccact
     2401 cgggcttttc gtagcggccg gtgacgcgca ggaacagatc gctgcccgcc gccaggcggc
     2461 tcgccagcgg caggtaatgg aacgggctga acgggttgcc gtaccagccg cggatcagct
     2521 cggccaggta gcggtacggc gtgagcgcgg tatgctgcgc ttcatagagg ttatagagca
     2581 tctaacatat tgttataaca gaattgacgc agtgccgcag tgatgaaact cctagcccgg
     2641 tcagcccttt ggggtgccct tctgctactc gccgccctgg cgggagggta cttgttcctg
     2701 cgccagtccc tgccccggac cgaaggggag ctgccggtgt cgggaatctc ccggccggtc
     2761 gagatcctgc gcgatgcctt cggcatcccg cacatctttg ccgagtcgat cgacgatgcg
     2821 cacttcgcgc tcggctacgt ccacgcccag gaccgcctgt ggcagatgga gatgaaccgc
     2881 cgcgtcgccg ccgggcggct cgccgaagtg ctcggtcccg gtgccctcga gtcggaccgc
     2941 ttcctgcgca cgcttggcgt gcatcgcgtt gcccgaagca acctggagca ctacgacgcc
     3001 gagacgcggc acgtgctcga cgcctacgcc gccggcgtca acgcttttct cgccggccgg
     3061 ccggtgctgc cgcccgagtt ctggctggcg cgcgtctccc ccgagccctg gtcgcctgcc
     3121 gattcggtgg gctggacgaa gatgatggcc tg
//
LOCUS       DASYJA010000468         3871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2975832, whole genome shotgun sequence.
ACCESSION   DASYJA010000468 DASYJA010000000
VERSION     DASYJA010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2975832"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VGO02_10160"
     CDS             <1..418
                     /locus_tag="VGO02_10160"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452232.1"
                     /note="catalyzes the formation of arginine from
                     (N-L-arginino)succinate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV7478087.1"
                     /translation="NQEDKEPLFDAVDTLKDCLAVSAGLVAGLQPVPRAMRAAALEGH
                     ATATDLADYLVRKGVAFRDAHEIVARAVREAERSGVELAKMPFSKLQKFSSKIGKDVT
                     RFLTLEGSVAARDHIGGTAPAQVRAAVARARKRLAR"
     gene            418..1713
                     /locus_tag="VGO02_10165"
     CDS             418..1713
                     /locus_tag="VGO02_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV7478088.1"
                     /translation="MERGGLTQIGKYPVLRKLGEGATSEVYLCNDPFNARDVAIKVAF
                     QETFDNPERGKLYRKLFLTEASLVGKLAHPHICQIYDAVADDKLSYIVMEHVGGGTLE
                     RFARPEGLLSIDRVVEMVFKCCRALEFAHKVGVTHRDIKPANILYLEGTEVKITDFGA
                     ALIATSRDTTQVSAIGSPAYMSPEQVKEHTLDHQTDIYSMGVVMYHLLTGRLPFQAVN
                     NFSLIYQITNVDPEPPSAYRPEIPPTIDAIVQHAMAKDLGDRYRQWEEFSFDLAEAFR
                     DERLHAHKAQEFADSDKFETLRKLPFFQHFSDAELWEVARISAWRHAAAGEQIMREGE
                     TGDFFCVIAQGQVKVTKKGKLLNLLRAGEPFGEMAYLSKHEPVRGADVTVMTDADIIS
                     VPTGMLAQASDACRHKFDRVFMEILVERLSMANLRLSGV"
     gene            1731..3008
                     /locus_tag="VGO02_10170"
     CDS             1731..3008
                     /locus_tag="VGO02_10170"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV7478089.1"
                     /translation="MEQIANYPVLRKLGEGATSEVFLCQDPFRGREVAVKRIFPETLR
                     DPARGRLFRKLFFTEASLAGKLDHPHIAQIYDAGIGEDSGYIVMEYVPGGTMERFCVP
                     ENLLPVEQTVEIAFKCARALAYAHAQGVTHRDIKPGNILYAATPIDVRIGDFGLAQKF
                     SAETTQITGIGSPAYMSPEQVREELVDHRTDLYSLGVVLYQLLTGQLPFRGANKYSII
                     YQITQQDPPPPSTLRAEVPPTLERIVRRAMQKDAARRYQSGDEMAQELIDALRAGLGE
                     PRREEFGDADKFAALRAMGFFTRFADPELWEVVRVGAWERVGAGAQLVHEGEPGNFFC
                     VLIEGEMRVTKNKKLLSLLGPGECFGEMAYLSLSSHERGASVTASKESRIVRIGVPDL
                     ERASADCRSKFDRAFIGILVERLNLANTRLTSG"
     gene            3005..3814
                     /locus_tag="VGO02_10175"
     CDS             3005..3814
                     /locus_tag="VGO02_10175"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEV7478090.1"
                     /translation="MNEQFKIDLQIERSARVTGGCIHECYRVSIRGKEWFLKANAIKH
                     EDAFAAEADALAGLRQAGYRAPAPHAHGVAAGRAYLLMEFLDLRGGVDFPALGRMLAQ
                     AHRGIGPDFGWDRDNYIGATPQANGWSDNWAKFWRERRMKPQLELAKRNGFSLPDVPL
                     QILNGHTPQPSLLHGDLWSGNASFTPIGPVVFDPAAYYGDREADLAMTELFGGFPPEF
                     YDAYNEAFPLPKEYEQRKHLYNLYHLLNHLNLFGRSYLGQVEETLGLLLRL"
BASE COUNT          673 a         1362 c         1244 g          592 t
ORIGIN      
        1 caaccaggag gacaaggaac ccctgttcga cgccgtcgac acgctcaagg actgcctggc
       61 cgtgagcgcg ggcctggttg ccggcctgca gccagtgccg cgcgcgatgc gcgccgccgc
      121 gctcgagggc cacgccacgg ccaccgacct cgccgactac ctggtgcgca agggcgtcgc
      181 cttccgcgac gcgcacgaga tcgtggcgcg cgcggtgcgc gaggccgagc gctccggggt
      241 ggagctggca aaaatgcctt tttcgaagtt gcagaaattt tcctcgaaaa tcggcaaaga
      301 cgtcacccgc ttcctgaccc tggaaggctc ggtcgccgcg cgcgaccaca tcggcggcac
      361 cgcgccggcg caggtgcgcg cggccgtggc gcgggcgcgc aagcgcctgg cccgctgatg
      421 gaacgcggcg gcctcacgca gatcggcaag tatccggtcc tgcgcaagct cggcgagggg
      481 gcgacctccg aggtctacct gtgcaacgac cccttcaacg cgcgcgacgt cgccatcaag
      541 gtggctttcc aggaaacctt cgacaacccc gagcgcggca agctgtaccg caagctgttc
      601 ctcaccgaag cctcgctcgt cggcaagctc gcccatcccc acatttgcca gatctacgac
      661 gcggtggccg acgacaagct gtcctatatc gtgatggagc acgtcggcgg cggtacgctc
      721 gagcgcttcg cgcggcccga ggggctgctg tcgatcgacc gcgtcgtcga gatggtgttc
      781 aagtgctgcc gcgcgctcga gttcgcgcac aaggtcggcg tcacgcaccg cgacatcaag
      841 ccggcgaaca tcctctacct ggagggcacc gaggtgaaga tcaccgactt cggcgcggcg
      901 ctcatcgcca cttcgcgcga caccacccag gtgagcgcca tcggctcgcc ggcctacatg
      961 tcgcccgagc aggtcaagga acacacgctc gaccaccaga ccgacatcta ctcgatgggc
     1021 gtggtgatgt accacctgct caccggccgg ctgccgttcc aggcggtcaa caacttcagc
     1081 ctgatctacc agatcaccaa cgtcgatccc gagccgcctt cggcgtatcg cccggagatc
     1141 ccgccgacca tcgacgccat cgtgcagcac gcgatggcca aggacctcgg cgaccgctac
     1201 cggcagtggg aggaattctc cttcgacctc gccgaggcct tccgcgacga gcgcctgcac
     1261 gcgcacaagg cgcaggagtt cgccgactcc gacaagttcg agacgctgag gaagctgccg
     1321 ttcttccagc atttctccga cgccgagctg tgggaggtgg cgcgcatctc cgcctggcgg
     1381 cacgccgcgg ccggcgagca gatcatgcgc gagggcgaga ccggcgattt cttctgcgtc
     1441 atcgcccagg gccaggtcaa agtgacgaag aagggcaagc tgctcaacct gctgcgcgcg
     1501 ggcgagccgt tcggcgagat ggcctacctc tccaagcacg agccggtgcg cggcgccgac
     1561 gtcaccgtga tgaccgatgc cgacatcatc tcggtgccca ccggcatgct ggcgcaggcc
     1621 tccgacgcct gccgccacaa gttcgaccgc gtcttcatgg agatcctggt cgagcggctg
     1681 tcgatggcca acctgcgcct gtcgggcgtc taggaagcgc gggcgggccg ttggaacaga
     1741 tcgcgaacta cccggtgcta cgcaagctgg gcgaaggcgc gacctccgaa gtgttcctgt
     1801 gccaggaccc gttccgcggc cgcgaggtgg cggtgaagcg catctttccc gagacgctgc
     1861 gcgacccggc gcgcggccgc ctgttcagga agctgttctt caccgaggcg tcgctcgccg
     1921 gcaagctcga ccatccgcac atcgcgcaga tctacgacgc cggcatcggc gaggattccg
     1981 gctacatcgt gatggagtac gttcccggcg gcacgatgga gcgcttctgc gtgccggaga
     2041 acctgctgcc ggtcgagcag acggtcgaga tcgcgttcaa gtgcgcgcgc gccctcgcgt
     2101 acgcgcatgc ccagggcgtc acgcaccgcg acatcaagcc cggcaacatc ctgtacgccg
     2161 cgacgccgat cgacgtgcgc atcggcgact tcggcctggc gcagaagttc agcgccgaga
     2221 ccacgcagat caccggcatc ggctcgcccg cctacatgtc gcccgagcag gtgcgcgagg
     2281 agctggtcga tcaccgcacc gacctctact cgctgggcgt ggtgctctac cagctgctca
     2341 ccgggcagct gccgttccgc ggcgccaaca agtacagcat catctaccag atcacgcagc
     2401 aggacccgcc gccaccctcc acgctgcgcg ccgaagtgcc gccgacgctc gagcgcatcg
     2461 tgcgccgcgc catgcagaaa gacgccgcgc gccgctacca gagcggcgac gagatggcgc
     2521 aggagctgat cgatgcgctg cgcgccgggc tgggcgagcc gaggcgcgag gaattcggcg
     2581 acgccgacaa gttcgccgcg ctgcgcgcca tgggcttctt cacgcgcttc gccgaccccg
     2641 agttgtggga agtggtgcgc gtcggcgcct gggagcgcgt cggcgccggc gcccagctcg
     2701 tgcacgaggg cgagccgggc aacttcttct gcgtgctgat cgaaggcgag atgcgcgtca
     2761 ccaagaacaa gaagctgctc tcgctgctcg gccccggcga gtgcttcggc gagatggcct
     2821 acctttcgct ctccagccat gagcgcggcg cctcggtcac ggcgagcaag gaatcgcgca
     2881 tcgtgcgcat cggcgtcccc gacctcgagc gcgcctcggc cgactgccgc agcaagttcg
     2941 accgcgcctt catcggcatc ctggtggagc gcctgaacct cgcaaacacc cggttgacct
     3001 caggttgaac gagcaattca aaatcgatct gcagatcgaa aggtccgcgc gtgtcaccgg
     3061 gggctgcatc cacgagtgct accgcgtttc gatccggggc aaggagtggt ttctcaaggc
     3121 caacgccatc aagcacgagg acgccttcgc cgcggaggct gacgccctgg ccgggctgcg
     3181 ccaggccggc taccgcgcgc cggcgccgca cgcgcacggc gtcgcagccg gccgcgccta
     3241 cctcctgatg gagttcctcg atctgcgggg cggcgtcgac ttccccgcgc tcggccgcat
     3301 gctggcgcag gcgcaccgcg ggatcgggcc ggacttcggc tgggaccgcg acaactacat
     3361 cggcgcaaca ccgcaggcaa acggctggtc ggacaactgg gcgaagttct ggcgcgagcg
     3421 gagaatgaag ccccagctcg agctggcgaa gcgcaatggt ttttctctac ccgacgttcc
     3481 actgcaaatt ctgaacggcc atacacccca gccctccctg ctgcacggcg atctatggag
     3541 cggcaacgcg agcttcacgc cgatcgggcc ggtggtgttc gacccggccg cctactacgg
     3601 cgaccgcgag gcagacctcg cgatgacgga gctgttcggg ggatttccac cggagttcta
     3661 cgacgcctac aacgaagcgt ttccgctacc caaggagtat gaacagcgca agcatctcta
     3721 caacctgtac cacctgctga accacctcaa cctgttcggc cgcagctatc tgggccaggt
     3781 cgaggaaacg ctaggcctgc tgcttcgcct ctagcagctt ctgcagctcg ccggactcgt
     3841 acatctcgcg catgatgtcg gagccgccga c
//
LOCUS       DASYJA010000469         1747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2158546, whole genome shotgun sequence.
ACCESSION   DASYJA010000469 DASYJA010000000
VERSION     DASYJA010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2158546"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VGO02_10180"
     CDS             <1..600
                     /locus_tag="VGO02_10180"
                     /inference="COORDINATES: protein motif:HMM:NF022673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HEV7478091.1"
                     /translation="VPPGPSDPYYEVQLFETGRVQTRAGSLHDLFNALAWLAFPRTKA
                     LINALHAEEIPRENGRRGRRRDLLTLLDEGGAIVQCDDAELIALVCGFRWKRLFWEQR
                     TRVQRHMRFRVLGHATLEQALQPRPGISCKAIFVPFDADADAGAHAWLAALPADATPE
                     LAVPLPVFGYPDWLADNRRPEFYDDERYFRPFRERRAFP"
     gene            complement(597..1109)
                     /locus_tag="VGO02_10185"
     CDS             complement(597..1109)
                     /locus_tag="VGO02_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013951778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEV7478092.1"
                     /translation="MRRRLYFLLPDVESARRTGDDLLLARVEDRHMHFLAKRGTDLAE
                     LHEAGYLIKTDCLHGAGVGLGLGALGGAVLGALIVLNPIEGTRPHPLWFFVAVLAGAL
                     LGAWVASMVGASVPNSKLRQFQREIEAGKVLLMVDVPCDAVDDLREVVNARHPEAVPA
                     GQVRPYPVFP"
     gene            1183..1532
                     /gene="rnpB"
                     /locus_tag="VGO02_10190"
     ncRNA           1183..1532
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGO02_10190"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          308 a          592 c          585 g          262 t
ORIGIN      
        1 gtgccgcccg gaccttccga cccgtactac gaggtccagc ttttcgagac cggccgggta
       61 cagacgcggg caggcagcct gcacgacctc ttcaatgcgc tcgcctggct cgcctttccg
      121 aggacgaaag cgctgatcaa cgcgctgcac gccgaggaaa tcccccgcga aaacggccgc
      181 cgcggccgcc ggcgcgatct gctgacgctg ctcgatgaag gcggcgcgat cgtccagtgc
      241 gacgacgccg agctcatcgc gctggtgtgc ggctttcgct ggaagaggct gttctgggag
      301 cagcgcaccc gcgtgcagag gcacatgcgc ttccgggtgc tcggccacgc gacgctcgaa
      361 caggcgctgc agcccaggcc gggcatctcg tgcaaggcga ttttcgtccc gttcgacgcc
      421 gatgcggacg caggggcgca cgcctggctc gccgcgcttc ccgcggacgc cacgccggag
      481 cttgccgtcc cgctgccggt gttcggctat ccggactggc tcgccgacaa ccgccgcccg
      541 gagttctacg acgatgaacg ctacttccgc cccttcaggg aaagacgggc gttcccctag
      601 gggaacaccg gatagggtct tacctgcccg gccggcaccg cctcgggatg ccgcgcattg
      661 accacttcgc ggaggtcatc gaccgcgtcg caaggcacgt ccaccatcag caataccttg
      721 cccgcctcga tctcgcgctg gaactggcgc agcttcgagt tgggtacgga cgcgccgacc
      781 atgctcgcca cccacgcgcc gagcagcgcg ccggccagca cggcgacgaa gaaccagagc
      841 gggtgcggcc gggtgccctc gatcgggttc agcacgatca acgcgccgag cacggcgccg
      901 ccgagcgcgc ccaggccgag cccgacgccc gcgccatgca ggcaatcggt cttgatgaga
      961 tatccggcct cgtgcagctc ggcgagatcg gtgccgcgct tcgcgagaaa gtgcatgtgg
     1021 cggtcttcca cgcgcgcgag cagcaggtcg tcgcccgtgc ggcgggcgct ttcgacatcg
     1081 ggaagaagaa agtaaaggcg cctgcgcatg atcaacctcc tggagcgctc ctggtgcggt
     1141 tgcggcttta taggttccgc ctgcggcggt aaaattgcaa cgggagtcgg ccaggcagcc
     1201 gctgtgcgcg agcacagagg aaagtccggg ctccgcagag cgggatgcag ggtaacgccc
     1261 tggcacggcg acgtgacgaa gagggccaca gagacgagtc ctcggccaag cagcatcggg
     1321 ccgaggggtg aaacgcggca acctccatcc ggagcaacac caaataggca agcgcggtgt
     1381 gaacaaaatc acactacaag gccggctcgg ccgagcttgc gggtaggtgg ctagagccgt
     1441 gacgtaagtc acggcccaga ggaatggctg ccggcgtggc gggggcaact ctgtcgcggg
     1501 cacagaaccc ggcttaccgg ccggctccca ttggtactcg gttcccattg ccactctctt
     1561 cgccggcaga tactctcgca ggccatgcac gacaggctca atcggaacga cgtcacgaat
     1621 cgcgtgaacg tggaggatcc gcagcgcgtg cgcgacgcgg tcgtcgcgct tctcgccgcg
     1681 cgctatcccg gcagcgattt caccgcgctg gcacgggcat tcgacgatct ccaggcgttg
     1741 ttcgagg
//
LOCUS       DASYJA010000470         2590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2067998, whole genome shotgun sequence.
ACCESSION   DASYJA010000470 DASYJA010000000
VERSION     DASYJA010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2067998"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VGO02_10195"
     CDS             <1..691
                     /locus_tag="VGO02_10195"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEV7478093.1"
                     /translation="IYSCLKWARENAHAVRWQITSEMWETLNATWLEMRAIPRGRVTG
                     SGATQFFDWVKDRSHLFRGVTYGTIVRGEAFNFSRLGTFLERADNTARILDVKYHILL
                     PSVADVGGALDYYQWAALLRSVSSFETYRQLYRDQIFPIKVAQLLILERRMPRSLAAC
                     FDQVKEALDRTRGEHDAAAKRLGYELHARLTHADIEEIFSGGLHEYLSECLEDIDELG
                     NRIQRAYLGMV"
     gene            688..1431
                     /locus_tag="VGO02_10200"
     CDS             688..1431
                     /locus_tag="VGO02_10200"
                     /inference="COORDINATES: protein motif:HMM:NF019977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEV7478094.1"
                     /translation="MKYTILHSLSYAYDSPVRSSTQYLRLTPRDTARQKVLGWKLEAP
                     GQPMHTQDGYGNVLHVMTIDRAVSGIMIRSRGTVQTSPTPDEPSDFTGTPLSPLLFLR
                     ATPLTRADGKLAALADRFRSRAGTLAGLRELSAAVLEQQAGGSAADLSHAFIACCHHL
                     GVPARYVSGYAYSAQSTDPQVAMHAWVEAWIAERWRSFDVAQNGPIGETHIKIAVGAN
                     YLDTCPVRGVRTGGGIETLLAQASVVANQ"
     gene            1469..>2590
                     /locus_tag="VGO02_10205"
     CDS             1469..>2590
                     /locus_tag="VGO02_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEV7478095.1"
                     /translation="MSLHVALNHVTHYRYDRPIVLSPQVVRLRPAPHARTPVLAYSLK
                     VAPAKHFVNWQQDPQANWLARYVFPEKTRELRIEVDLVAEMSVINPFDFFLEPHAEKF
                     PFKYDPAQLQELESYLRKDPLTPLFKAYLDEIPRTPKPTNDFLVELNRQVARDVRYLI
                     RMEPGVQTPEQTLELASGSCRDSGWLLAQLLRHLGIATRFVSGYLIQLKPDQRSLDGP
                     SGTDVDFTDLHAWCEAYLPGAGWVGLDPTSGLLAGEGHIPLACTPEPSSAAPITGLTE
                     KCEVEFSHQMSVRRVLEAPRVTKPYTEAQWLAIEQLGHRIDADLVAHDVRLTMGGEPT
                     FVSIDNPDGAEWNTEAMGPRKRLLGSALLKRLKGRYAPKG"
BASE COUNT          434 a          929 c          815 g          412 t
ORIGIN      
        1 gatctactcc tgcctgaaat gggcgcgcga gaacgcgcac gccgtgcgct ggcagatcac
       61 ctccgaaatg tgggagacgc tgaacgccac ctggctcgag atgcgcgcca tcccgcgcgg
      121 ccgcgtgacc ggctcgggcg cgacgcagtt cttcgactgg gtgaaggacc gctcgcatct
      181 gttccgcggc gtgacctacg gcaccatcgt gcgcggcgaa gcgttcaact tctcccgcct
      241 gggcaccttc ctcgagcgcg ccgacaacac cgcgcgcatc ctcgacgtga agtaccacat
      301 cctgctgccc tcggtcgccg acgtcggcgg cgcgctcgac tactaccagt gggcggcgct
      361 gctgcgctca gtgtcgtcgt tcgagaccta tcgccagctc taccgcgacc agatcttccc
      421 gatcaaggtg gcgcagctgc tgatcctcga gcggcgcatg ccgcgctcgc tcgccgcctg
      481 cttcgaccag gtaaaggagg cgctcgaccg cacgcgcggg gagcatgacg ccgccgccaa
      541 gcgcctgggc tacgagctgc acgcgcgcct gacgcatgcc gacatcgagg agatcttcag
      601 cggcgggctg cacgaatact tgagcgagtg cctggaggac atcgacgagc tcggcaaccg
      661 catccagcgc gcctacctgg ggatggtatg aagtacacca tcctgcactc gctcagctat
      721 gcctacgact cgccggtgcg ctcctccacg cagtacctgc gcctgacgcc gcgcgacacc
      781 gcgcgccaga aggtgctcgg ctggaagctc gaggcaccag gccagccgat gcacacgcag
      841 gacggctacg gcaacgtgct gcacgtcatg accatcgacc gagcggtctc cggcatcatg
      901 atccgctccc gcggcacggt gcagacctcg cccacgcccg acgagccctc cgacttcacc
      961 ggcacgccgc tctcgccgct actgttcctg cgggctacgc cgctgacgcg cgccgacggc
     1021 aagctcgccg cgctggccga tcgcttccgg agccgcgccg gcacgctcgc cgggctgcgc
     1081 gagctgtcgg cggccgtgct cgagcagcag gcgggaggaa gcgccgcgga cctgtcgcat
     1141 gccttcatcg cctgctgcca ccatctcggc gtgccggcgc gctacgtctc gggctatgcc
     1201 tactccgcgc agagcaccga cccgcaggtc gcgatgcacg cctgggtcga ggcctggata
     1261 gcggagcgct ggaggagctt cgacgtggcg cagaacggcc cgatcggcga gacgcacatc
     1321 aagatcgcgg tcggcgccaa ttacctcgac acctgcccgg tgcgcggcgt gcgcaccggc
     1381 ggcggtatcg aaacccttct cgcgcaagca agcgtggtgg ccaaccaatg agtcgccgct
     1441 tcttttgccc ccctgttaag gtcccgcaat gagcctccac gtcgcgctga accatgtcac
     1501 ccattatcgg tacgaccgcc cgatcgtgct gagtccgcag gtcgtgcgct tgcgtccggc
     1561 tccgcacgcg cgcacgccgg tgctcgccta ctcgctgaaa gtcgcgccgg ccaagcactt
     1621 cgtcaactgg cagcaggacc cgcaggcgaa ctggctggcg cgctacgtgt tcccggagaa
     1681 gacccgcgag ctgcgcatcg aggtcgacct ggtcgccgag atgtcggtga tcaatccgtt
     1741 cgatttcttc ctcgagccgc acgccgagaa gttcccgttc aagtacgacc cggcgcagct
     1801 ccaggagctg gaaagctatc tgaggaagga tcccctgacg ccgcttttca aggcctacct
     1861 cgacgagatc ccgcgcaccc ccaagccgac caacgatttc ctggtcgagc tcaaccgcca
     1921 ggtcgcgcgc gacgtgcgct atctcatccg catggaaccg ggcgtgcaga cgccggagca
     1981 gacgctcgag ctggcgagcg gttcgtgccg cgactcgggc tggctgctgg cgcagctcct
     2041 gcgccacctg ggaattgcca ctcgctttgt ttccggatat ttgattcagt taaaaccaga
     2101 tcagcgttct ttggacggtc cttcgggaac cgacgtcgac ttcaccgact tgcacgcctg
     2161 gtgcgaggcc tacctgcccg gcgccggctg ggtcggcctc gacccgacct cggggctgct
     2221 cgccggcgag ggccacatcc cgctcgcctg cacgcccgag ccctcgagcg ccgcgccgat
     2281 caccggcctc accgagaaat gcgaggtcga gttctcgcac cagatgagcg tgcggcgcgt
     2341 gctcgaggcg ccgcgcgtca ccaagcccta caccgaagcg caatggctcg cgatcgagca
     2401 gctcggacat cgcatcgacg ccgacctcgt ggcgcacgac gtgcggctca ccatgggcgg
     2461 cgagccgacc ttcgtctcca tcgacaaccc agacggcgcg gaatggaaca cggaggcgat
     2521 gggtccgaga aagcgtctcc ttggttccgc gttgctgaaa agattgaagg gacgctacgc
     2581 gccgaagggg
//
LOCUS       DASYJA010000471         4080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1431939, whole genome shotgun sequence.
ACCESSION   DASYJA010000471 DASYJA010000000
VERSION     DASYJA010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431939"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VGO02_10210"
     CDS             <1..281
                     /locus_tag="VGO02_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7478096.1"
                     /translation="AIAQGMRGHIDAEFPMGPDVLRGIDLWNIWGKVACPTLVLRGAE
                     SDVLPPSILREMKERKPDMESIEFAGVGHAPALMSDDQISAVKRFLLR"
     gene            complement(250..603)
                     /locus_tag="VGO02_10215"
     CDS             complement(250..603)
                     /locus_tag="VGO02_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HEV7478097.1"
                     /translation="MKQGIVLVAHGSRDPGWSRPFERLAAALEKKLPAVAVALCYLEH
                     GPSLEEALLALVAKGVGAIRVVPVFLGAGGHVKQDIPKLVAAANPGVPVSVDSPIGEQ
                     DQLIEAIAEGIASPR"
     gene            complement(605..1270)
                     /locus_tag="VGO02_10220"
     CDS             complement(605..1270)
                     /locus_tag="VGO02_10220"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEV7478098.1"
                     /translation="MSSGRVFRFAPTPEHRTNHLFVFLHGCGATAQSMVPIALQFQAR
                     FPSAALVVPSGFDPDARGGAAQEWYPTRGLTIDNHRERVAAVLPDVDDLVRREQTHFG
                     VPAGRTVLIGFSQGGTVALEAVKAPGLAGAVVAFSARYARLPNNGTHISSRIHLVHGE
                     FDSVVSRVYAERAARVLSGLHVPVTLDIVEDLGHALSRQAIGLGSLRLLQGIYEGRRA
                     TLH"
     gene            1337..3034
                     /locus_tag="VGO02_10225"
     CDS             1337..3034
                     /locus_tag="VGO02_10225"
                     /inference="COORDINATES: protein motif:HMM:NF017775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEV7478099.1"
                     /translation="MKNTGFLKAFWALAKPYWVSEQRAKGWTLLGMVVGLALMLVWLE
                     VQFNTWNNDFYNTLQDKDQAEFFRQLGRFGLLALIYIVTAVYRLYFQQMLQIEWRTWL
                     TERFLQDWMKDQAYYRMQLLDHGTDNPDQRIAEDLNLFVGNTLSLSLGLLSAVVTLVS
                     FIAILWTLSGVLEVAGFAIPGYMVWIALVYAVIGSVLTHYIGKPLIGLDFNQQRFEAD
                     FRFSLVRLRENGEGVALYRGESEELGGFRSRFGAVISNWWALMLKQKQLTWFTSFYAQ
                     AAIIFPYIVVAPRFFSGAMQLGGIFQTASAFGQVQGALSWFIGAYTQYATWKATVDRL
                     VGFSATLQRVRDEARAVAGERSAGAGDALSLDQLAISLPKGQPLLAPSSLQLRKNENV
                     IVTGPSGSGKSTLFRTLAGIWPYWKGRIRLPQGARLLFLPQKPYLPIGTLKHAVSYPD
                     DVSRHPDEETRGALEAVGLAHLAADLERNENWAQTLSGGEQQRLAFARALLNKPDWLF
                     LDEATASLPEDAQDALYRVLAERLPRTTLVSIGHRAGLAAHHARRLAWSGQSLVAAG"
     gene            complement(3031..>4080)
                     /locus_tag="VGO02_10230"
     CDS             complement(3031..>4080)
                     /locus_tag="VGO02_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478100.1"
                     /translation="WFYQRAQSSEIYQTLFFSALYLALLNFLRPLKERGPAGLDARAW
                     RWLAAAWTCAALLVFIRVVNGLLLPLIVLLAVWCAASGRSWRELRGSAARLGAALLVP
                     PALIVITLGVVNQVKFGSPWLTGYHQWSPETHLPIGRLADGVWGYLFAPRFSIFLYFP
                     LLVFALFGLRRFAGRYRLDLAVMLTIFTVFFLVLARTPSWAGEWTYGPRYLLPMLPVL
                     SLPFLAFADGVLERIGTWRARAWAAAALACLAYSAYLQVQVNLLPFFIYYNAREALSA
                     SRSLDAIDYFLYRHTALIAQDLVRHRYDVEGLPYFAEMKRSGPPEFVAEYRRNFGAML
                     ERGNLYWALPPGQRH"
BASE COUNT          713 a         1339 c         1411 g          617 t
ORIGIN      
        1 cggcgatcgc gcagggaatg cgcggccaca tcgacgccga gttcccgatg gggccggacg
       61 tgctgcgcgg catcgacctg tggaacatct ggggcaaggt ggcctgcccg acgctcgtgc
      121 tgcgcggcgc cgagtccgac gtgctgccgc cgagcatcct gcgcgagatg aaggagcgca
      181 agcccgacat ggagagcatc gagttcgccg gcgtgggcca cgctccggcg ctgatgagcg
      241 acgaccagat cagcgcggtg aagcgattcc ttctgcgata gcctcgatca gctgatcctg
      301 ctcgccgatc gggctgtcga cgctcaccgg cacgccgggg ttagccgccg caaccagctt
      361 ggggatgtcc tgctttacgt gcccgcccgc gccgaggaac accggcacga cacggattgc
      421 gccgacgccc tttgccacca gcgccagaag cgcttcttcg agcgaagggc cgtgctcgag
      481 gtagcacagc gcgaccgcga ccgcaggcag cttcttttcc agggcggcgg cgaggcgctc
      541 gaaggggcgt gaccagcccg gatcgcgcga gccgtgggcg acgaggacga tgccttgttt
      601 caactcagtg cagcgtggcg cgccggcctt cgtagatgcc ctgcagcagc cgcagcgagc
      661 ccaggccgat cgcctggcgc gacagcgcgt ggccgaggtc ctcgacgata tccagggtca
      721 ccgggacgtg caggcccgag agcacgcgcg cggcgcgctc cgcatagacg cgcgagacca
      781 ccgagtcgaa ctcgccgtgc accagatgga tgcgcgagga aatgtgcgtg ccgttgttgg
      841 gaagccgcgc gtagcgcgcg gagaacgcga ccaccgcgcc ggcgaggccg ggcgccttga
      901 ccgcctcgag cgccacggtg ccgccctgcg agaagccgat cagcacggtg cggcccgccg
      961 gcaccccgaa atgggtctgc tcgcgccgca ccaggtcgtc gacgtcgggc agcaccgccg
     1021 caacgcgctc gcggtggttg tcgatggtca ggccgcgcgt cgggtaccac tcctgcgccg
     1081 cgccgccgcg cgcgtcggga tcgaagccgc tcggcaccac cagcgccgcg ctcgggaagc
     1141 gcgcctggaa ctgcagcgcg atcggcacca tggactgcgc cgtggcgccg cagccgtgca
     1201 ggaagacgaa caggtggttg gtccggtgct caggagtggg cgcgaagcga aagacccgtc
     1261 cggaagacat gcccccagat tgttaacccg tgatgacgct tctgtaaact gatttgcatt
     1321 gcctcttagg tacgccatga aaaacaccgg atttctaaag gctttctggg ctctggccaa
     1381 gccctattgg gtctcggagc agcgcgcgaa gggctggacg ctgctcggca tggtggtcgg
     1441 gctcgctctg atgctggtct ggctcgaggt gcagttcaat acgtggaaca acgacttcta
     1501 caacacgttg caggacaagg accaggccga gttcttccgc cagctcggca ggttcggcct
     1561 gctcgccctc atctacatcg tcaccgcggt gtatcgcctc tatttccagc agatgctgca
     1621 gatcgagtgg cgcacctggc tcaccgagcg cttcctgcag gactggatga aggaccaggc
     1681 ctactaccgc atgcagctcc tcgatcacgg caccgacaac cccgaccagc gcatcgccga
     1741 ggacctgaat ctgttcgtcg gcaatacgct ctcgctcagc ctgggtctgc tgtcggcagt
     1801 cgtcacgctg gtgtcgttca tcgcgatcct gtggacgctg tccggggtgc tcgaggtggc
     1861 cggcttcgcg atacccggct acatggtgtg gatcgcgctg gtctacgccg tcatcggctc
     1921 ggtgctcacc cactacatcg gtaagccgct catcggcctg gatttcaacc agcagcgctt
     1981 cgaggccgac ttccgttttt cgctggtacg cctgcgagag aacggcgagg gcgtggcgct
     2041 gtaccgcggc gagtccgagg agctgggcgg cttccgcagc cgcttcggcg cggtcatcag
     2101 caactggtgg gcgctgatgc tcaagcagaa gcagctcacc tggttcacct cgttctatgc
     2161 gcaggctgcg atcatcttcc cctatatcgt ggtggcgcca cgcttcttct ccggcgcgat
     2221 gcagctcggc ggcatcttcc agaccgcgtc ggccttcggc caggtgcagg gcgcgctgtc
     2281 ctggttcatc ggcgcctaca cccagtacgc gacctggaag gcgaccgtgg accgcctggt
     2341 cggtttctcc gcgacgctgc agcgggtcag agacgaggcg cgcgccgtcg ccggcgagcg
     2401 cagcgcgggc gcgggcgatg cgctgtcgct ggaccagctc gcgatctcgc tgccaaaagg
     2461 ccagccgctg cttgccccgt cctctctgca attgaggaaa aacgagaatg tcatcgtgac
     2521 cgggccgtcg ggctccggca aatcgacgct gttccgcacg ctggcgggaa tctggccgta
     2581 ctggaagggg cgcatccgcc tgccgcaggg cgcgcggctg ctgttcctgc cgcagaagcc
     2641 ctacctgccg atcggcacgc tgaagcacgc ggtgagctat ccagacgatg tttcacgcca
     2701 ccccgacgaa gaaaccagag gcgcgctgga agcggtggga ttggcgcacc tggcggcgga
     2761 tctcgagcgc aacgagaatt gggcgcagac gctgtccggc ggcgagcagc agcggctcgc
     2821 gttcgcgcgg gcgctgctca acaagccgga ctggctgttc ctcgacgagg cgacggcctc
     2881 gctgcccgag gacgcgcagg acgcgctcta ccgggtgctg gcggaaaggc tgccgcgcac
     2941 cacgctggtt tcgatcggcc atcgcgcagg actcgccgcg catcatgcgc gccggctcgc
     3001 ctggagcggg caatcgctgg tggcggcggg ctagtggcgc tggccgggcg gcagcgccca
     3061 gtagagattg ccgcgctcga gcatggcgcc aaagttgcga cggtactcgg cgacgaactc
     3121 gggcgggcca gagcgcttca tctccgcgaa gtacggcaac ccctcgacat cgtagcgatg
     3181 ccgcaccagg tcctgcgcga tcagcgcggt atggcggtaa agaaagtaat cgatggcgtc
     3241 cagcgagcgc gatgcggaga gcgcttcgcg cgcgttgtag tagatgaaga acggcagcag
     3301 attcacttgc acctgcaggt aggctgaata ggcgaggcag gcgagcgcgg ccgccgccca
     3361 ggcgcgtgcc cgccaggtac cgatacgctc gagcacgccg tcggcgaagg cgagaaacgg
     3421 cagcgacagc acgggcagca tgggcagcag gtaacgcggg ccataggtcc attccccggc
     3481 ccaagatggc gtcctggcga gcaccaggaa aaagaccgtg aaaatggtca gcatcacggc
     3541 caggtcgagg cggtagcggc cagcgaaccg gcgcaggccg aaaagcgcaa ataccagcag
     3601 tggaaagtag agaaagatgc tgaagcgcgg cgcgaagagg tagccccaca ccccgtcggc
     3661 caggcgcccg atcggcagat gggtttccgg gctccactgg tggtagccgg tcagccaggg
     3721 cgagccgaac ttcacctggt tgaccacgcc gagcgtgatg acgatcagcg ccggcggtac
     3781 gaggagcgcc gcgccgagcc tggctgcgct gccccgcagc tcgcgccagg agcggccgct
     3841 cgccgcgcac cagaccgcga gcaggacgat cagcggcagc agcagcccgt tgacgacgcg
     3901 aatgaacacg agcagcgcag cgcaggtcca ggctgccgcc agccagcgcc aggcgcgcgc
     3961 gtcgaggccc gccggcccgc gttccttgag ggggcgcagg aagttgagca gggcaaggta
     4021 gagggcgctg aagaacagcg tctggtagat ctccgagctc tgcgcccgct ggtagaacca
//
LOCUS       DASYJA010000472         6771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2976416, whole genome shotgun sequence.
ACCESSION   DASYJA010000472 DASYJA010000000
VERSION     DASYJA010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2976416"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="VGO02_10235"
     CDS             <1..998
                     /locus_tag="VGO02_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7478101.1"
                     /translation="QWEWGARFALECLPGRFERNCRTLAGLAAYSRDSLRELRGRAGL
                     QYDQLSRGILHFATALPDFEAMARHAEAMRSLGAERQVKSAAECSALEPALAHSQQPV
                     VGGVFTPEDESGDAHRFTRELARLAAARGVTFQFGMAVEALEGAGGKIVALRTHDGAR
                     MSADAYVVSLGSYSPLLLKPLGIRIPVYPLKGYSITLALGPGEAAAAPSVSLTDEAHK
                     IVISRLGTRLRAAGTAELAGYDTSVSEVRCAAIVRRIRQLFPRLAAAPAVDNWAGLRP
                     ATPNNVPLIGKTRLANLFLNTGHGTLGWTLACGSARALADLVSGRRPQIGFDFLA"
     gene            991..1851
                     /locus_tag="VGO02_10240"
     CDS             991..1851
                     /locus_tag="VGO02_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7478102.1"
                     /translation="MRNSDSVFFDIRGLRYHVRRWPKAGAPKMVLLHGWMDVSASFQF
                     LVDALARDWDVYAPDWRGYGLTGWGRADCYWFPDYIADLDLLLETIEKNPVNLVGHSL
                     GGNVAAMYAGIRPARVAKFVNLEGFGMQATRPEQAPKRYARWLDELREASPLRPYESF
                     AALADRLQKNNARLSREKAEFLARHWGREEGGGVVLRGDPAHKVVNPVLYRYEEVRAC
                     WQQVAAPVLWVDAAGSDTLKRLGLDAAQHAERRAAFRNLKYATIEDAGHMLHHDQPEA
                     VARLIEEFLA"
     gene            1851..2483
                     /locus_tag="VGO02_10245"
     CDS             1851..2483
                     /locus_tag="VGO02_10245"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010116161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEV7478103.1"
                     /translation="MAQYFSIHPTHPQARLVRRAAEIVRGGGLIAYPTDSCYALGCHL
                     GDARAVERLRRVRHMDARHHLTLMCRDLSEIAAYAIVDDARFRLLKRATPGSYTFILR
                     ATREVPRRLAHPKRKTIGVRVPAHPVAHALLAELNEPMLSATLLLPGEAAPPTDPAQI
                     RSRLEHEVDLVVDAGFCGAEPTTVLDLTGDEAVVLRAGKGSLAPFSIESV"
     gene            2502..3185
                     /locus_tag="VGO02_10250"
     CDS             2502..3185
                     /locus_tag="VGO02_10250"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV7478104.1"
                     /translation="MDVPSLVQTVAIYALPVLFAITLHEAAHGYVARHFGDMTAHAEG
                     RISLNPLRHVDLNGTVIVPLVLIALSGGKFAFGWAKPVPVNYSALRNPKQHMAWVAAA
                     GPAVNLLMALGWALLLKSVVELPHGFASGFLAQMAIAGIVVNVVIMAINLLPILPLDG
                     GRILVGLLPRGLAREYAKTERWGGYVLLAFIGASLLDSWLYDGRYMGGVFAPLGILIE
                     NVLRTVVGR"
     gene            3191..4393
                     /locus_tag="VGO02_10255"
     CDS             3191..4393
                     /locus_tag="VGO02_10255"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEV7478105.1"
                     /translation="MYETRVLSGMRPTGSLHLGHYHGVLKNWVRLQAEYPCLFFVADW
                     HALTTDYAAPGDIPKNTWDMVIDWLAAGIDPNQAILFIQSQVADHAELHLLLSMMTPL
                     GWLERVPTYKDQQEKLADKDLSTYGFLGYPLLQSADILIYRAKYVPVGEDQVPHVEFT
                     REVARRFNHLYGREPGFEDKAKAAVKKLGTKKARLFNDLRTKFLESGDAEALKRAQAL
                     LDEQQNMPRGDRERLYGWLEGSGKKILVEPDALLTEASRMPGLDGQKMSKSYGNTISL
                     REEPESVAKKIKVMPTDPARVKRTDPGNPEKCPVWELHKIYSDEERKKWVQKGCTTAS
                     IGCLECKQPVIEAINAELAPMRERAQVYLDDPTLVKNIAADGCEKAKRLAAETMRDVR
                     EAMGLIYG"
     gene            4390..5166
                     /locus_tag="VGO02_10260"
     CDS             4390..5166
                     /locus_tag="VGO02_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEV7478106.1"
                     /translation="MMAAKLYGEPVIELPHDLYIPPDALEVVLEAFEGPLDLLLYLIR
                     RENLDILDIPMAPLTRQYLEYVEAMRSKNLELAADYLVMAAMLMEIKSRLLLPRPPSA
                     APIEEDPRAELVRRLLEYEQIKKAAHALDELPQLGRDVIAISVWIEKTVAERLPDVEA
                     QDLAEAWRGLLHRARLSAHHHVSREELSVRAHMSGILRALRERRVLEFAELFDPGRGV
                     AVLVVTFLALLELARELLIEITQSECFAPIYVKLGHAQPG"
     gene            5150..5779
                     /gene="scpB"
                     /locus_tag="VGO02_10265"
     CDS             5150..5779
                     /gene="scpB"
                     /locus_tag="VGO02_10265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEV7478107.1"
                     /translation="MHSPAETKRILEAALLTAQEPLSVSDLKKLFDEELGAEVLKALL
                     GEVREEWSGRAVELVQLASGWRFQTRAEFRPYVERLLPEKPPRYSRAVMETLAIIAYR
                     QPVTRGDIEDVRGVVVSTQIVQSLEARGWIDIVGHRETPGRPALYATTKKFLDDLGLR
                     TLEELPPLEEIAQTLQLEPAPAPAAEAPAAAGDEAGAEPEAERATGTGG"
     gene            5793..6494
                     /locus_tag="VGO02_10270"
                     /pseudo
     CDS             5793..6494
                     /locus_tag="VGO02_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004548140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            6561..>6771
                     /locus_tag="VGO02_10275"
     CDS             6561..>6771
                     /locus_tag="VGO02_10275"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEV7478108.1"
                     /translation="MSLENVIEPVITGMGYELVDVQASNGGRFLRLFIDKPQGINVDD
                     CAAVSRQLTRVFEVEGIDYDRLEVSS"
BASE COUNT         1093 a         2300 c         2320 g         1058 t
ORIGIN      
        1 cgcagtggga atggggcgcg cgcttcgcgc tggaatgcct gcccggccgc ttcgagcgca
       61 attgccggac gctggccggc ctcgccgcct acagccgcga cagcctgcgc gagctgcgcg
      121 gccgcgccgg cctgcagtac gaccagcttt cccgcggcat cctgcatttc gcgactgcgc
      181 tgcccgactt cgaggcgatg gcgcggcacg ccgaagccat gcgctccctc ggcgccgagc
      241 gccaggtcaa atcggccgcc gaatgctcgg cgctcgagcc ggcgctcgcg cactcgcagc
      301 agcccgtggt cggcggcgtg tttactcccg aggatgaatc cggcgatgcg catcgcttta
      361 cgcgcgagct ggcgcgcctc gcagctgcgc gcggcgtcac cttccagttc ggcatggcgg
      421 tggaagcgct cgaaggagcc ggcggaaaaa ttgttgccct gcggacgcat gatggcgcgc
      481 gaatgagtgc cgacgcctac gttgtatccc ttggcagcta cagccccttg ctgctcaagc
      541 cgctcggtat ccgtattcca gtctatccgc tgaagggcta ctcgatcacc ctcgcgctcg
      601 gccccgggga agcggcggcg gcgcccagcg taagccttac cgacgaggcg cacaagatcg
      661 tcatctcgcg gctcggcacc cggctgcgcg cggcgggcac cgccgagctc gccggctatg
      721 acacgagcgt gagcgaggtg cgctgcgccg cgatcgtgcg ccgcatccgg cagctcttcc
      781 cgcggctggc tgccgcgccc gcggtcgaca actgggcggg gctgcgcccg gcgacgccca
      841 acaacgtgcc gctcatcggc aagacgcggc tcgccaacct gttcctcaat accggccacg
      901 gcaccctggg atggacgctc gcctgcggct cggcgcgcgc gctggcggat ctcgtctcgg
      961 ggcgccgtcc gcagatcggg ttcgacttcc ttgcgtaaca gcgactcggt tttcttcgac
     1021 atccgcggcc tgcgctacca cgtgcggcgc tggccgaagg ctggcgcgcc gaagatggtt
     1081 ctgctgcacg gctggatgga cgtctcggct tcgttccagt tcctggtcga cgcgctcgcg
     1141 cgcgactggg atgtctacgc gccggactgg cgcggctatg gcctgaccgg ctggggcagg
     1201 gccgactgct attggtttcc cgactacatc gcagatctcg atttgctctt ggaaacgata
     1261 gaaaagaatc ctgtgaatct ggtgggccac agcctcggcg gcaacgttgc cgcgatgtac
     1321 gccggtatcc ggccggcgcg agtggcgaag ttcgtgaacc tggaaggctt cggcatgcag
     1381 gcgacgcggc cggagcaggc gccgaagcgc tacgcccgct ggctcgacga gctgcgcgag
     1441 gcttcgccac tgcggcctta cgagagcttc gccgcgctgg ccgaccgcct gcagaagaac
     1501 aacgccaggc tgtccaggga aaaggccgaa ttcctcgcgc gccattgggg gcgggaggag
     1561 ggcggcggcg tcgtgctgcg cggcgatccc gcgcacaagg tcgtcaatcc ggtcctctac
     1621 cgctacgagg aggtgcgcgc ctgctggcag caggtcgcgg ctcccgtgct ctgggtcgac
     1681 gccgctggtt ccgataccct gaaacggctg ggactcgacg cggctcagca cgcggagcgc
     1741 cgcgcagcgt tccgcaacct gaagtacgcg acgatcgagg acgccggcca catgctgcac
     1801 cacgaccagc ccgaggccgt ggcccgcctg atcgaagagt tcctcgccta atggcgcagt
     1861 atttctcgat ccatcccact cacccgcagg cgcggctggt gcggcgcgcc gctgagatcg
     1921 tgcgcggtgg cgggctgatc gcctacccga ccgactcctg ctacgcgctg ggctgccatc
     1981 tcggggacgc ccgcgcggtc gagcgcctgc gccgcgtgcg ccacatggat gcgcgccacc
     2041 acctgacgct gatgtgccgc gacctgtcgg agatcgccgc ctacgccatc gtcgacgacg
     2101 cgcgctttcg cctcctcaaa cgcgcaacgc ccggcagcta tacgttcatc ctgcgcgcca
     2161 cgcgcgaggt gccgcggcgc ctggcgcatc cgaagcgcaa gacgatcggc gtgcgggtgc
     2221 cggcgcaccc cgtggcgcac gcgctgctgg ccgagctgaa cgagccgatg ctctcggcga
     2281 cgctgctgct gccgggcgaa gcggcgccgc ccaccgatcc ggcgcagatc cgcagccgcc
     2341 tggagcatga ggtggacctg gtcgtcgacg cgggcttctg cggcgccgag ccgaccacgg
     2401 tgctcgacct taccggcgac gaagccgtcg tgctgcgcgc agggaagggc tcgctcgcgc
     2461 cgttttcgat cgagagcgtc tgataaaatc cgcgctcaaa gatggacgtc ccttccctcg
     2521 ttcagacggt cgccatctac gccctcccgg tgctgttcgc tatcacgctg cacgaggcgg
     2581 cgcatggcta tgtcgcgcgg catttcggcg acatgacggc gcacgccgaa gggcgcatca
     2641 gcctcaaccc cctgcgccac gtcgacctca acggcacggt catcgtgccg ctggtcctga
     2701 tcgcgctgtc gggcggaaag ttcgccttcg gctgggccaa gcctgtgccg gtcaactact
     2761 ccgcgctgcg caaccccaag cagcacatgg cctgggtggc cgccgccggg ccggcggtga
     2821 acctgctgat ggcgctgggc tgggcgctgc tgctcaagtc ggtcgtcgag ctgccgcacg
     2881 gcttcgccag cgggtttctc gcgcagatgg cgatcgcggg aatcgtcgtg aacgtggtca
     2941 tcatggcgat caacctgctg ccaatcctgc cgctggatgg cggcaggatc cttgtcgggc
     3001 tgctgcccag ggggctggcg cgcgaatacg cgaagaccga gcgatggggc ggctacgtcc
     3061 tgctggcgtt catcggtgcg agcctgctcg attcctggct ctatgatgga aggtatatgg
     3121 gaggggtgtt cgcgcccctg gggatcctca tcgaaaacgt gctgcgcacc gtagtaggca
     3181 gataagaaga atgtacgaaa cgcgcgtact ttccggcatg cggccgacgg gcagcctgca
     3241 cctgggccac taccacggcg tgctgaagaa ctgggtccgg ctgcaggccg agtacccgtg
     3301 cctgttcttc gtcgccgact ggcacgcgct gaccaccgac tacgccgcac ccggcgacat
     3361 cccgaagaac acctgggaca tggtcatcga ctggctggcg gcggggatcg acccgaacca
     3421 ggcgatcctg ttcatccagt cgcaggtggc ggaccacgcc gagctgcacc tgctgctgtc
     3481 gatgatgacg ccgctcggct ggctcgagcg cgtgcccacc tacaaggacc agcaggagaa
     3541 gctggccgac aaggaccttt cgacctacgg cttcctcggc tatccgctgc tgcagtcggc
     3601 cgacatcctc atctaccgcg ccaagtacgt tccggtcggc gaggaccagg tgccgcacgt
     3661 ggaattcacg cgcgaggtgg cgcgccgctt caaccacctc tacggccgcg agccgggctt
     3721 cgaggacaag gccaaggcgg cggtgaagaa gctcggtacc aagaaggcgc gtctgttcaa
     3781 cgatctgcgc accaagttcc tggagagcgg cgacgccgag gcgctcaagc gcgcgcaggc
     3841 gctgcttgac gagcagcaga acatgccgcg cggcgaccgc gagcggctgt acggctggct
     3901 ggagggcagc ggcaagaaga tcctggtcga gcccgacgcg ctgctgaccg aggcctcgcg
     3961 catgccgggc ctcgacgggc agaagatgtc gaagtcctac ggcaacacga tctcgctgcg
     4021 cgaggagccc gagtcggtgg cgaagaagat caaggtcatg ccgaccgacc cggcgcgcgt
     4081 caagcgcacc gacccgggca atcccgagaa gtgcccggtg tgggagctgc acaagatcta
     4141 ttcggacgag gagcgcaaga agtgggtgca gaagggctgc accacggcgt cgatcggctg
     4201 cctggagtgc aagcagccgg tgatcgaggc gatcaacgcg gagctcgcgc cgatgcgcga
     4261 gcgcgcgcag gtctacctcg acgacccgac gctggtgaag aacatcgccg ccgacggctg
     4321 cgagaaggcg aagcggctgg cggccgaaac catgcgcgat gtgcgcgaag ccatgggcct
     4381 gatctacgga tgatggccgc caagctctac ggcgagccgg tcatcgagct gccgcacgac
     4441 ctgtacattc cgcccgacgc cctggaagtc gtgctggagg cgttcgaggg cccgctcgac
     4501 ctgctgctct acctgatccg cagggagaac ctcgacattc tcgacatccc catggcgccg
     4561 ctcacgcgcc agtacctcga gtacgtcgag gcgatgcgct cgaagaacct cgagctcgcc
     4621 gccgactacc tggtgatggc cgccatgctg atggagatca agtcgcgcct gctgctgccg
     4681 cggccgccgt cggccgcgcc gatcgaggag gatccgcgcg ccgagctggt gcgccgcctg
     4741 ctcgagtacg agcagatcaa gaaggcggcg cacgccctcg acgagctgcc gcagctggga
     4801 cgcgacgtca tcgccatctc ggtgtggatc gagaagaccg tagccgagcg cctgcccgac
     4861 gtcgaagcgc aggacctggc ggaggcctgg cgcggactgc tgcaccgcgc gcgcctgtcg
     4921 gcgcaccacc acgtctcccg cgaggagctc tcggtgcgcg cgcacatgag cggcatcctg
     4981 cgcgcgctgc gcgagcggcg cgtgctcgag ttcgccgagc tgttcgaccc ggggcgcggc
     5041 gtggcagtgc tggtggtcac cttcctcgcc ctgctcgagc tggcgcgcga gctgctgatc
     5101 gagatcacgc agtccgaatg cttcgcgccg atttacgtaa aattgggcca tgcacagccc
     5161 ggctgaaacc aagcgcatcc tcgaggcggc tctgctcacc gcgcaggagc cgctgagcgt
     5221 gtccgacctg aagaagctgt tcgacgagga gctgggcgcc gaggtcctga aggccctgct
     5281 cggcgaggtg cgcgaggagt ggagcgggcg cgccgtggag ctggtgcagc tggcgagcgg
     5341 ctggcgcttc cagacgcgcg ccgagttccg gccctacgtc gagcgcctgc tcccggaaaa
     5401 gccgccgcgc tactcgcgcg cggtgatgga aacgctcgcc atcatcgcct accgccagcc
     5461 ggtcacccgc ggcgacatcg aggatgtcag aggggtggtc gtgtcgacgc agatcgtgca
     5521 gtcgctggaa gcgcgcggct ggatcgacat cgtcggccac cgcgagacgc ccgggcgtcc
     5581 ggcgctgtac gccacgacca agaaattcct cgacgacctc gggctgcgca cgctcgagga
     5641 gctgccgccg ctcgaggaga tcgcgcagac cctccagctt gaacccgcac cggccccagc
     5701 cgccgaagcg cccgctgctg cgggcgacga agccggcgca gagcccgaag ccgagcgcgc
     5761 caccggcacc ggcggctgac ccgcgcggtg aacggttgca gaagctgctg gccgcggctg
     5821 gcttcggctc gcgtcgggaa atcgagacct ggatcgaggc cggtcgcatc gcggtcggcg
     5881 gcaaggtcgc caagctcggc gaccgcgcgc tgccctccga cgcggtcacc ctggatggga
     5941 aaagcctaac gctttcagca gcttcgggca cgcgtgtcct cctctacaac aagccggtcg
     6001 gcgagctggt cacccgcagc gacccggcag gccgccccac cgtatttacc cggctgccac
     6061 caggcaggtg ggttgcagtc ggccgcctcg acttcaacac gtcaggcctt ctgctgctga
     6121 cagattcggg cgagctggcc aatcgcctca tgcacccgcg ctacgagctc gagcgcgagt
     6181 acgccgtgcg cgtgcacggc cagcctgact tgcaaaagct ccgcgccggc atcaccttgg
     6241 atgacggccc ggctgctttc gatcgcatcg agcccgcgcc ggggcgctcc gccgagggcg
     6301 cgaaccgctg gtatcgcgtc gtgctgcggg aaggccgcaa ccgcgaagtc cggcgcctgt
     6361 tcgaagccgt aggcacccag gtgagccgcc tgatcagggt tcggtacggg ccggtcgagc
     6421 tgcccccgga tctggcggcg ggaacatggc gcgagctggc tgccgagcgc gtttccgggt
     6481 tgttcctgaa gtagaaatcg gttaaacttc aaaagctttg gtggtgcagc gtaacagatg
     6541 ggcttcggcc cattttttgt ttgagcctcg aaaacgtgat cgagccggtg ataaccggca
     6601 tggggtacga gctggtcgac gtgcaggcgt cgaacggcgg gcggtttctg cgcctgttca
     6661 tcgacaagcc acaaggcatc aacgttgacg attgcgccgc ggtcagccgg cagttgacgc
     6721 gggtgttcga ggtcgagggc atcgactacg acaggctgga agtgtcctcg c
//
LOCUS       DASYJA010000473         4374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_980174, whole genome shotgun sequence.
ACCESSION   DASYJA010000473 DASYJA010000000
VERSION     DASYJA010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980174"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VGO02_10280"
     CDS             <1..217
                     /locus_tag="VGO02_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7478109.1"
                     /translation="EFTPRAHVAAPGTQDLCFIASTSLDNVVSQLKKHNVNIIEGPVA
                     KTGATGPIRSVYVRDPDLNLVEISVAA"
     gene            complement(203..334)
                     /locus_tag="VGO02_10285"
     CDS             complement(203..334)
                     /locus_tag="VGO02_10285"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEV7478110.1"
                     /translation="MLRKLLDWIAVALRERHAYNELRRLDDRMLRDIGLDRGRLGGH"
     gene            408..1109
                     /locus_tag="VGO02_10290"
     CDS             408..1109
                     /locus_tag="VGO02_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7478111.1"
                     /translation="MPERVLIVEDDREVREAVADYLASHGYDCAQAGDGKAMRVALAE
                     SAPDLVLLDLRLPGEDGLSLARWLRDNHEVAIIMVTAAGDVVDRVVGLEVGADDYLGK
                     PFDLRELLARVKSVLRRGGKPEKKPAKAGATQRVPIGACQLDLDTHQLIGQKGEEIPL
                     TGMEFDLLRVFSEHPNRVLSRDQLLTLTRNREWEPFDRSIDIRIARLRRKIELDPEKP
                     RTIKTVRGAGYIFIP"
     gene            1172..3937
                     /locus_tag="VGO02_10295"
     CDS             1172..3937
                     /locus_tag="VGO02_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV7478112.1"
                     /translation="MDFHWERLKPVLDHAPVVVFARDLQGRYLYVNLAFQKLIGKGPE
                     EIIGRSMDEVLPPEVAGTIRDSDREVIDTGQGVVVEAIAVYGSGRRTIMNFKFPLPGA
                     DGRAEAVLGIGTDVTDRRRREEALQAAALAVSSAKGDAVFQELTRYLAVILNAELALI
                     GRFTDKSPRTVQTLGMFGGGAYRENFEYPLDITPCRNVVDGSFSVVPRDVVREYPQDE
                     YLQTMGAESYAGFPLKDGDGNIAGVIAVVSKKPLVDQHLIESVLKIFAVRAGAEIERR
                     DHEEGLRKSESQYRSIFDATTDSLVLRDADFRIVDVNPAYEAMSGRQRAEVLGAGALT
                     MSVPEMNEYVRGLHDQVLAGEPVVFETQAIKKDGTRFEMETRGVPIMHQGRPHVLYIG
                     RDISARKSEEELLRASEEQYRAIFNAAADALVLRDPDARIVDVNRAFLEISGFTRGEV
                     LSESRWIFALPEMSALAKDMHRRVIAGESVHFEIKARRKDGAPIDIEMRAVPIRYRGR
                     PHALGMARDITARKREEAERAELEAQLRQAQKMEAIGHLTGGIAHDFNNILQGILGNL
                     TLAQEHVESQRDAKLDRYLERAHVSASRARELIPQMLTFSRGKRGAPRVLALPPLVAE
                     AAQLLRPALPSSIELETRLEENVGAVRADPVQLEQVLLNLCINARDAMAGTGQIALAV
                     RTAPERSGVCTSCRQKFAGRFVELSVRDSGPGIAPGVIDRMFEPFYSTKEVGKGSGMG
                     LSMVHGIVHEHRGHLIVDTMRGRGSTFRILLPDAEEAIQEFRLERQQSRAKARLSGSV
                     MVVDDEDVILEFIGDLLQGWGLDVMLMPNGVDAQRAFAAQPQRYDLVLTDQTMPRLTG
                     LELARRIRGIRADTPVILYTGYGEEISDAELDSAGVRALARKPVEPAELLTLVRTHLK
                     QKGNVLK"
     gene            3969..4361
                     /locus_tag="VGO02_10300"
     CDS             3969..4361
                     /locus_tag="VGO02_10300"
                     /inference="COORDINATES: protein motif:HMM:NF022592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HEV7478113.1"
                     /translation="MDPIETCTEKHMDGYLVHGGMSMARGSTVRIEDGAGILVYVWEG
                     ELWVTQEGDSRDHFVSPGRWFRLERNGLSLLYATRRTHATLTAPTAEAYARLITATPA
                     GTTVPRVLYDGSRERKTWLELKLGHFFR"
BASE COUNT          742 a         1496 c         1499 g          637 t
ORIGIN      
        1 ggagttcacg ccgcgcgcgc acgtcgcggc gccgggcacg caggacctgt gcttcatcgc
       61 ttcgacctcc ctcgacaacg tcgtttcaca gctgaaaaag cacaacgtga acatcatcga
      121 gggcccggtg gcgaaaaccg gggccaccgg cccgatccgc tcggtctacg tgcgcgaccc
      181 ggacctgaac ctggtcgaga tctcagtggc cgcctaggcg gccgcggtcg aggccgatgt
      241 cgcgcagcat gcggtcgtcg agccggcgca gctcgttgta ggcgtgccgc tcgcgcaggg
      301 cgacggcgat ccagtcgagg agttttctga gcatagtgag gacaggttaa ggctcgcctg
      361 taagtttccc gtcgtggatt tgtagcgggt ttgtatacaa tcgcgtcatg cccgagcgcg
      421 tgctgatcgt cgaggatgac cgcgaagtgc gcgaggcagt cgccgactac ctcgcctcgc
      481 acggctacga ctgcgcgcag gcgggcgatg gcaaggcgat gcgcgtggcg ctcgcggaaa
      541 gcgcccccga cttggtgctc ctcgacctgc gcctgcccgg cgaagacggc ctgtcgctcg
      601 cccgctggct gcgcgacaac cacgaggtgg cgatcatcat ggtgacggcc gccggcgacg
      661 tggtggaccg cgtcgtcggc ctggaagtcg gcgccgacga ttacctcggg aagcccttcg
      721 acctgcgcga gctgctggcg cgggtgaaaa gcgtgctgcg ccgcggcggc aagccggaaa
      781 agaagcctgc aaaggcgggg gcgacccagc gcgtgccgat cggcgcctgc cagctcgacc
      841 tcgacacgca ccagctgatc ggccagaagg gcgaggagat cccgctcacc ggcatggaat
      901 tcgatctgct gcgcgtcttc tccgagcacc ccaaccgtgt gctgtcgcgc gaccagctcc
      961 tcacgctgac ccgcaaccgc gagtgggagc cgttcgaccg ctcgatcgac atccgcatcg
     1021 cccgcctgcg ccgcaagatc gagctcgacc cggagaagcc gcgcaccatc aagacggtgc
     1081 gcggggcggg atatattttc atcccgtaac gggtttgttt cagtcctgta attccccgct
     1141 ggaaaaccgc tagttcaata gactgcccgg catggatttc cactgggagc ggctcaagcc
     1201 ggtgctcgac cacgcgccgg tcgtcgtgtt cgctcgcgac ctgcagggcc gctacctgta
     1261 cgtcaacctc gccttccaga agctgatcgg caagggcccg gaggagatca tcggccgcag
     1321 catggacgag gtgctgccgc cggaggtcgc cggcaccatc cgcgattccg accgtgaagt
     1381 gatcgatacc ggccagggcg tggtggtgga ggcgatcgcg gtgtacggct cgggccggcg
     1441 caccatcatg aacttcaagt tcccgctgcc cggcgccgac ggccgcgccg aggcggtgct
     1501 cggcatcggc accgacgtca ccgaccgccg ccggcgcgag gaggcgctgc aggccgccgc
     1561 gctcgccgtg tcgagcgcga agggcgacgc ggtgttccag gagctgacgc gctatctcgc
     1621 cgtaatcctg aacgccgagc tggcgctgat cggtcgcttc accgacaagt cgccgcgcac
     1681 ggtgcagacg ctgggcatgt tcggcggcgg tgcctaccgc gagaacttcg agtacccgct
     1741 cgacatcact ccctgccgca acgtggtcga cggcagcttc tcggtggtgc cgcgcgacgt
     1801 ggtgcgcgag tacccgcagg acgagtacct gcaaacgatg ggcgccgaaa gctatgccgg
     1861 ctttccgctc aaggacggcg acggcaacat cgccggggtc atcgccgtcg tgtcgaagaa
     1921 gccgctggtc gaccagcacc tgatcgagtc ggtgctcaag atcttcgccg tgcgcgccgg
     1981 cgccgagatc gagcggcgcg accacgagga gggcctgcgc aagagcgagt cgcagtaccg
     2041 ctcgatcttc gacgccacca ccgactcgct ggtgctgcgc gacgccgact tccgcatcgt
     2101 cgacgtcaac ccggcgtacg aggcgatgag cggcaggcag cgcgccgaag tgctcggcgc
     2161 cggcgccctc accatgagcg tgccggagat gaacgagtac gtgcgcggcc tgcacgacca
     2221 ggtgctcgcc ggcgagccgg tggtgttcga gacccaggcg atcaaaaagg atggcacgcg
     2281 cttcgagatg gagacgcgcg gcgtgccgat catgcaccag ggccggccgc acgtgctgta
     2341 catcggccgc gacatctcgg cgcgcaagag cgaggaggag ctgctgcgcg cgagcgagga
     2401 gcagtaccgc gccatcttca acgccgccgc cgacgcgctg gtgctgcgcg accccgacgc
     2461 gcgcatcgtc gacgtcaacc gcgccttcct cgagatcagc ggcttcacgc gcggcgaggt
     2521 gctgtccgag agccgctgga tcttcgccct gccggagatg tcggcgctgg cgaaggacat
     2581 gcaccggcgc gtgatcgccg gcgagtcggt gcacttcgag atcaaggcgc ggcgcaagga
     2641 cggcgcgccc atcgacatcg agatgcgcgc ggtgccgatc cgctaccgcg gccggccgca
     2701 cgcgctcggc atggcgcgcg acatcaccgc gcgaaaaagg gaggaagcgg agcgcgcgga
     2761 gctcgaagcg cagctgcgcc aggcacagaa gatggaggcc atcggccacc tgaccggcgg
     2821 catcgcgcac gacttcaaca acatcctgca gggcatcctg ggcaacctca cgctggcgca
     2881 ggagcacgtc gaaagccagc gcgacgccaa gctcgaccgc tatctcgagc gcgcccacgt
     2941 ctcggcgagc cgcgcgcgcg agctgatccc gcagatgctc accttcagcc gcggcaagcg
     3001 cggcgcgccg cgcgtgctcg ccctgccgcc gctggtcgcc gaggcggcgc agctgctgcg
     3061 cccggcgctg ccctcgagca tcgagctcga gacgcggctc gaggagaacg tcggcgcggt
     3121 gcgcgccgac ccggtgcagc tggagcaggt gcttctcaac ctgtgcatca acgcgcgcga
     3181 cgcgatggcc gggaccggcc agatcgccct cgccgtgcgc accgcgcccg agcgctccgg
     3241 cgtgtgcacc tcgtgccggc agaaattcgc cggacggttt gtggagctgt cggtgcgcga
     3301 cagcgggccg ggcatagcgc ccggggtgat cgaccgcatg ttcgagccct tctactcgac
     3361 caaggaggtc ggcaagggca gcggcatggg cctgtcgatg gtgcacggca tcgtgcacga
     3421 gcaccgcggc cacctcatcg tggacacaat gcgtggcagg ggctcgacct tccgcatcct
     3481 gctgcccgac gccgaggaag ccatccagga attcaggctc gagcggcagc agagccgcgc
     3541 gaaggcgcgg ctctcgggca gcgtgatggt ggtcgacgac gaggacgtga tcctggagtt
     3601 catcggcgac ctgctgcagg gctggggcct ggatgtaatg ctgatgccga acggcgtcga
     3661 tgcgcagcgc gccttcgccg cccagccgca gcgctacgac ctggtgctca ccgaccagac
     3721 catgccgcgc ctcaccggcc tggagctcgc ccgccggatc cgcggcatcc gcgccgacac
     3781 gccggtgatc ctctataccg gctatggcga agagatctcc gacgccgagc tcgactcggc
     3841 aggcgtgcgg gcgctcgccc gcaagccggt ggaacccgcg gagctgctca cgctggtgcg
     3901 cacccacctg aaacaaaaag gcaacgtcct gaaataaccc tgcgaccttg gctggctagc
     3961 ctctctgcat ggacccgatt gaaacatgca cggagaagca catggacggc tacctggtac
     4021 acggcggaat gagcatggcg cgcggcagca ccgtgcgcat cgaggacggc gcgggcatcc
     4081 tggtctacgt ctgggaaggc gagctgtggg tcacgcagga aggcgactcg cgcgaccatt
     4141 tcgtcagccc cggccgctgg ttccgcctgg agcgcaacgg cttgtcgctg ctttacgcca
     4201 cgcggcgtac ccacgccacg ctcaccgcgc cgaccgccga ggcctacgcc cggctgatca
     4261 cagcgacgcc agccggtacc accgtacccc gggtcctcta cgacggttcg cgcgagcgca
     4321 agacctggct ggaactcaag ctaggtcact tcttcaggta gtcgggggtg ccgg
//
LOCUS       DASYJA010000474         3911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2886904, whole genome shotgun sequence.
ACCESSION   DASYJA010000474 DASYJA010000000
VERSION     DASYJA010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2886904"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..721)
                     /locus_tag="VGO02_10305"
     CDS             complement(122..721)
                     /locus_tag="VGO02_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEV7478114.1"
                     /translation="MVKGRERGFTYLTALFLVAILAGGLALAGTVWEQAAAREREAEL
                     LFVGHQYRRAIGLYYDSTPGSVKRYPQALEDLLKDPRQPATQRFLRKLYPDPFGGKEW
                     GVVKGSDGGIAGVYSLSEAKPIKVSGFKVRDAAFEGAQKYADWKFTHTPASAAPGTKP
                     PAGAAPVAPGAAPIAVPPGAPPVIPPAADGAPSPEGGDH"
     gene            complement(715..1089)
                     /locus_tag="VGO02_10310"
     CDS             complement(715..1089)
                     /locus_tag="VGO02_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEV7478115.1"
                     /translation="MRLRRGFTLIELLVVLSVIAILLTLAVPRYFGSIDKSKEAVLKE
                     NLNQMRDAISRYYADKGKYPESLDSLAADKYLRKVPIDPITESSATWQVMRPDDPQKG
                     GVYDVKSGAPGKGLDGSEFSQW"
     gene            complement(1100..1564)
                     /locus_tag="VGO02_10315"
     CDS             complement(1100..1564)
                     /locus_tag="VGO02_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEV7478116.1"
                     /translation="MKGFTLIELVVTVAIVAVLASIALPFNELVVQRSKEQDLRRALR
                     EMRDAIDAYKQASDEGRLQKRVGDSGYPRKLEDLVAGVEDQKNPKKERIYFLRRLPRD
                     PFAIDATLGPAETWGKRSYASPPDDPREGDDVFDVFSLATGTGINGRPYREW"
     gene            complement(1561..3462)
                     /locus_tag="VGO02_10320"
     CDS             complement(1561..3462)
                     /locus_tag="VGO02_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEV7478117.1"
                     /translation="MRRTLFPAVLLLAACATSSPVDEARKRMSEGKSEEALNILQQAI
                     KDDPASPAKGEYYRLRDVLATQWLAQAETLRATGQFEAADVLYRRVQAYDPANQRAAA
                     GAAQIEVDRRHRTVVAEADQLAKAGKLREAQDVLRPVLNENPQNRDARRLQRAIDDKL
                     VKPVIVSAQLRPSSLKPISIELREVTLRNVFEVLQRASGINFVFDRDVRADQRTTILL
                     RDASIEESIRMVLLTNQLEQKVLNDNTVFVYPNTPQKLREYQELVVKGFYLANADVKQ
                     TANMIRTMVKTRDIFIDEKINLLVLKDTPAAIRLAERLIAAQDLAEPEVMLEVEVLEV
                     GANKMLQLGIRYPDTLAVSLQGAAAIPGRVTLPEWQNRSSDLVQLQFNDPLFLFSLHQ
                     DDGSTTVLANPRIRVKNKEKARIHIGDRVPVITTTAAATGGFVSESVSYLDVGLKLEV
                     EPLIYLEDEVGIKVGLEVSNIVKEIRTVGSNTLSYQIGTRNAATVLRLRDGETQVLAG
                     LISNEDRQTAVRVPGLGEMPVIGRLFSNTTDSRTKTEIVLLITPRLLRQIARPDAASV
                     EFAAGTEASSGAPRLGGAAPIGAAPSSPTQFEGAPPPGDEPLRVPAPIAPPSGSTMVP
                     FGGVKPAPQ"
     gene            complement(3459..>3911)
                     /locus_tag="VGO02_10325"
     CDS             complement(3459..>3911)
                     /locus_tag="VGO02_10325"
                     /inference="COORDINATES: protein motif:HMM:NF022201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HEV7478118.1"
                     /translation="FYVSALAPLEQEAAAQRLALERLKTRTPHRPVSSGGREEDLRRF
                     QALFPSASELTDEVDRLHRLARRAGLDLAQGEYRLERRVSGLWAYRVTLPVRGSYPQF
                     RDFIGAVLKDMPIASIDALRFERKRAAETQLEAQVRVTLYARPPGETP"
BASE COUNT          524 a         1323 c         1417 g          647 t
ORIGIN      
        1 gctgcgcgtg ggcaagcacc gcgtcgagta tccgcaggaa gtggtggatg ccatgccgcc
       61 ggcattgcgc aaggcctttg ccgccagccg gaacaggatc cggcgctcct agctgtacct
      121 gctagtggtc gccgccctcg ggcgagggcg caccgtcggc cgccggcgga atgaccggcg
      181 gggcgcccgg cgggacggcg atgggtgccg cacccggtgc gacgggcgcc gcccctgccg
      241 gcggcttggt tccaggcgcg gcgctggcgg gcgtgtgcgt gaatttccag tcagcgtact
      301 tctgcgcgcc ctcgaacgcg gcgtcgcgca ccttgaagcc ggaaaccttg atcggcttgg
      361 cctcggacag gctgtagacg ccggcgatgc cgccgtcgga acccttcacc acgccccatt
      421 ccttgccgcc gaacgggtcg gggtagagct tgcgcaggaa gcgctgcgtc gccggctggc
      481 gcgggtcctt gagaagatcc tccagcgcct gcgggtagcg cttcaccgag ccgggcgtgc
      541 tgtcgtagta caggccgatg gcgcgccggt actggtggcc gacgaagagc agctccgctt
      601 cgcgctcgcg cgccgccgcc tgctcccata ccgtgccggc gagcgcgagc ccgccggcga
      661 gtatcgccac caggaacagc gccgtgagat aggtaaagcc gcgctcccgg cctttcacca
      721 ttgcgagaac tcgctgccgt cgaggccctt gcccggggcg ccgctcttca cgtcgtagac
      781 gccgcccttc tgcggatcgt ccggacgcat cacctgccag gtggcgctgc tctcggtgat
      841 cgggtcgatc ggcaccttgc gcaggtactt gtccgcggcg agggagtcga gcgactccgg
      901 gtacttgccc ttgtcggcgt agtagcgcga gatggcgtcg cgcatctggt tgaggttctc
      961 cttcagcacc gcttccttcg acttgtcgat gctgccgaag tagcgcggca cggcaagcgt
     1021 gaggaggatc gcgatgaccg atagaaccac cagcagctcg atcagtgtaa agccgcgccg
     1081 tagccgcatc acaatccctc taccattccc tgtatggccg cccgttgatg ccggtcccgg
     1141 tcgccagcga gaagacgtcg aacacgtcgt cgccctcgcg cgggtcgtcc ggcgggctgg
     1201 cgtagctgcg cttgccccag gtctcggccg ggccgagcgt ggcgtcgatg gcgaacggat
     1261 cgcgcggcag gcggcgcagg aagtagatgc gctccttctt cgggttcttc tggtcctcga
     1321 cgccggcgac caggtcctcg agcttgcgcg gatagcccga gtcgccgacg cgcttctgca
     1381 ggcggccctc gtcggaagcc tgcttgtagg cgtcgatcgc gtcgcgcatc tcgcgcagcg
     1441 cgcggcgcag gtcctgctcc ttcgagcgct gcaccaccag ctcgttgaag ggcagggcga
     1501 tcgaggcgag caccgcgacg atcgccacgg ttaccaccag ctcgatgagg gtaaagcctt
     1561 tcactgcggc gccggcttca ctccgccgaa gggcaccatg gtcgagcccg aggggggcgc
     1621 gatcggcgcc ggcacgcgca gcggctcgtc gccgggcggc ggcgcgccct cgaactgcgt
     1681 cggcgaggag ggcgcggccc cgatgggggc ggcgccgccc aggcgcggcg cgcccgagga
     1741 ggcctcggtg ccggcggcga actcgaccga ggcggcgtcg gggcgcgcga tctggcgcag
     1801 caggcgcggc gtgatgagca gcacgatctc ggtcttggtg cggctgtcgg tggtgttgga
     1861 gaacagccgc ccgatcaccg gcatctcgcc caggccgggc acgcgcacgg cggtctgccg
     1921 gtcctcgttg ctgatcagcc cggccagcac ctgcgtctcg ccgtcgcgca gccgcaggac
     1981 ggtggcggcg ttgcgcgtgc cgatctggta ggaaagcgtg ttcgagccga cggtgcggat
     2041 ctccttcacg atgttgctca cctcgagccc gaccttgatg ccgacctcgt cctcgaggta
     2101 gatcagcggc tcgacctcga gcttgaggcc gacgtcgagg tagctgaccg attccgagac
     2161 gaagccgccg gtggccgccg cggtggtggt gatgaccggc acgcggtcgc cgatgtggat
     2221 gcgcgccttc tccttgttct tgacgcggat gcgcggattg gcgagcaccg tggtgctgcc
     2281 gtcgtcctgg tgcaggctga agaggaacag cgggtcgttg aactgcagct gcaccaggtc
     2341 cgagctgcgg ttctgccact cgggcagcgt cacccggccg gggatggccg cggcgccctg
     2401 caggctgacg gccagcgtgt cggggtagcg gatgccgagc tgcaacatct tgttggcgcc
     2461 gacctcgagc acctcgacct ccagcatcac ctcgggctcg gccaggtcct gcgccgcgat
     2521 caggcgctcg gccaggcgga tcgccgccgg ggtgtccttc agcaccagca ggttgatctt
     2581 ctcgtcgatg aagatgtcgc gcgtcttcac catggtgcgg atcatgttgg cggtctgctt
     2641 gacgtcggcg ttggcgaggt agaagccctt caccaccagc tcctggtact cgcgcagctt
     2701 ctgcggcgtg ttcgggtaga cgaacacggt gttgtcgttc agcaccttct gctcgagctg
     2761 gttggtgagc agcaccatgc ggatcgactc ctcgatactg gcgtcgcgca gcaggatggt
     2821 ggtgcgctgg tcggcgcgca cgtcgcggtc gaacacgaag ttgatgccgg aggcgcgctg
     2881 caggacctcg aacacgttgc gcagcgtcac ctcgcgcagc tcgatcgaga tcggcttcag
     2941 gctgctcggg cgcagctgcg ccgagacgat caccggcttc accagcttgt cgtcgatggc
     3001 gcgctgcagg cggcgcgcgt cgcggttctg cgggttctcg ttgagcaccg ggcgcagcac
     3061 gtcctgcgcc tcgcgcagct tgccggcctt cgccagctgg tcggcctcgg ccaccaccgt
     3121 gcggtggcgg cggtcgacct cgatctgcgc cgcgccggcc gcggcgcgct ggttcgccgg
     3181 gtcgtacgcc tgcacgcggc ggtagagcac gtcggcggcc tcgaactggc cggtggcgcg
     3241 cagcgtctcg gcctgcgcca gccactgcgt ggcgagcacg tcgcgcaggc ggtagtactc
     3301 gcccttcgcc gggctcgccg gatcgtcctt gatcgcctgc tggaggatgt tgagggcctc
     3361 ctcgctcttg ccctccgaca tgcgcttgcg cgcctcgtcg accggagagg acgtcgcgca
     3421 ggcggcgagc agcagcacgg caggaaacag ggttcttctc atggcgtttc tccggggggc
     3481 cgtgcataca gcgtgacgcg cacctgggct tcgagctggg tctcggcggc gcgcttgcgc
     3541 tcgaagcgca gcgcgtcgat cgaggcgatc ggcatgtcct tcagcaccgc gccgatgaag
     3601 tcgcggaact gcgggtagct gccgcgcacc ggcagcgtga cgcggtaggc ccacaggccg
     3661 ctgacgcggc gctcgaggcg gtactcgccc tgcgcgaggt ccagcccggc gcgccgcgcg
     3721 aggcggtgca ggcggtcgac ctcgtcggtg agctcggagg ccgacgggaa cagggcctgg
     3781 aagcgccgca ggtcctcctc gcggccgccg gagctgaccg ggcgatgcgg cgtgcgcgtc
     3841 ttcaggcgct cgagggcgag gcgctgcgcc gccgcctcct gctcgagcgg cgcgagcgcc
     3901 gagacgtaga a
//
LOCUS       DASYJA010000475         2216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1613677, whole genome shotgun sequence.
ACCESSION   DASYJA010000475 DASYJA010000000
VERSION     DASYJA010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613677"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VGO02_10330"
     CDS             complement(<1..383)
                     /locus_tag="VGO02_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478119.1"
                     /translation="MAILSQSVQAGAARSLVWVVAATVFVAVGALGGLALAVGELEAL
                     WISLSVVAALVVLVDYRIGAVLLILMLPIADSHLFPHALMGMTGVNPVNMLLAGTLVS
                     YVVRAGAGAQAGRLLQPQRIWLDMVP"
     gene            complement(386..541)
                     /locus_tag="VGO02_10335"
     CDS             complement(386..541)
                     /locus_tag="VGO02_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478120.1"
                     /translation="MRPERKLLLGAAVLIAAACSLGWRPASGLDQQGERIMQPSVLKQ
                     SALHTRS"
     gene            complement(565..1458)
                     /gene="epsG"
                     /locus_tag="VGO02_10340"
     CDS             complement(565..1458)
                     /gene="epsG"
                     /locus_tag="VGO02_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determinant protein tyrosine kinase
                     EpsG"
                     /protein_id="HEV7478121.1"
                     /translation="MLAKQTENVLPMDGPARAPGKGRHIGAILMDEGKLTPTDAEQVL
                     ARQRELGWRFGEAAIELNLITDADLRQALAKQYEFPYLASGPDGVSKELIAAWDPFHP
                     VVEELRSVRTQLLIRWFNPGGGRRMLVVASPGAREGRSFIAANLAVVFSQMGLRTLLV
                     DADFRAPRQQAIFNVSDRFGLSSALSGRADLSAAAPVTGLAGLAVLPAGPLPPNPLEL
                     LSRGAFPALLAKAQSEYDLILIDTPPINEYADVQSIVHRAGDVLMVSRKDQTLLADTE
                     RAARDLSDAAGRIVGTLMNAY"
     gene            complement(1471..>2216)
                     /locus_tag="VGO02_10345"
     CDS             complement(1471..>2216)
                     /locus_tag="VGO02_10345"
                     /inference="COORDINATES: protein motif:HMM:NF025179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HEV7478122.1"
                     /translation="RNATYEAISRQKQAAETLKGASIDSIPEVLSSGHISALKVDLGR
                     AEARLAELGEVLGPNHPQYQRTSAEVSGLREKVGTEMKRIVAGLGNTVEQARRREEEL
                     KTAVTAQAERVQVMKDARIEMAVMTRDVENAQRAYDAVLARFVTNKVDSAAKQTNVAI
                     LTPAVEPIKPMHPKLGLIAGLSVVVGLLLAAAIVYLLETLDRRVRSRADLESRLAVPS
                     FGRLSRWQPTGGRLLPVPLRTARALPHPW"
BASE COUNT          323 a          768 c          806 g          319 t
ORIGIN      
        1 ggcaccatgt ccagccagat gcgctgcggc tgcagcaggc ggccggcctg cgcgccggcg
       61 ccggcgcgca ccacgtagga gaccagcgtg ccggcgagca gcatgttcac cgggttgacg
      121 ccggtcattc ccatcagcgc gtgcgggaag agatggctgt ccgcgatcgg cagcatcagg
      181 atcagcagca ccgcgccgat gcggtagtcg accagcacga cgagcgccgc caccaccgac
      241 agcgagatcc acagggcctc gagctcgccg acggcgagcg ccaggccgcc gagcgcgccg
      301 accgcgacga atacggttgc ggcaacgacc cagaccaggc tgcgggctgc gccggcctgt
      361 accgactgcg acaggatggc catgctcagc tccgcgtgtg cagggccgac tgcttgagga
      421 cggaaggttg catgatgcgc tctccctgct gatccaggcc gctcgccggc cgccagccga
      481 gcgagcacgc cgcggcgatc agcaccgcgg cgcccagcaa cagcttgcgt tcgggtctca
      541 ttggttcgcc actccgcttt ttcctcagta cgcgttcatc agcgtgccga cgatgcgacc
      601 ggccgcatcg gacaggtcgc gcgccgcgcg ctcggtgtcg gcgagcagcg tctggtcctt
      661 gcgcgatacc atcagcacgt cgcccgcgcg atgcacgatg ctctgcacgt cggcgtattc
      721 gttgatcggc ggcgtgtcga tcaggatcag gtcgtactcc gactgcgcct tcgccagcag
      781 cgccgggaag gcgccgcgcg acaggagctc gagcggattg ggcggcaagg gaccggccgg
      841 cagcaccgcc aggccggcga ggccggtgac cggcgcggcg gccgaaaggt cggcgcgccc
      901 ggaaagcgcc gaggacaggc cgaagcggtc ggagacgttg aagatcgcct gctggcgcgg
      961 cgcgcggaaa tccgcgtcga ccagcagcgt gcgcagcccc atctgcgaga acaccaccgc
     1021 caggttggcg gcgatgaagc tgcggccctc gcgcgcgccg gggctggcga cgacgagcat
     1081 gcggcgcccg ccgccggggt tgaaccagcg gatcaggagc tgggtgcgca ccgagcgcag
     1141 ctcctcgacc accgggtgga acggatccca ggcggcgatc agctccttcg acacgccgtc
     1201 ggggccggac gccaggtagg gaaactcgta ctgcttggcg agcgcctggc gcaggtcggc
     1261 gtcggtgatc aggttgagct cgatcgccgc ctcgccgaag cgccagccca gctcgcgctg
     1321 gcgcgccagc acctgctcgg cgtcggtggg cgtgagcttg ccctcgtcca tcaggatggc
     1381 gccgatgtgg cggcccttgc cgggggcgcg cgccgggccg tccatcggca gtacgttctc
     1441 agtctgtttg gctagcatgg gattcaccgg ttaccaggga tggggaagcg cgcgggcggt
     1501 gcgcaggggc acgggaagca gccgaccgcc cgtcggctgc cagcgcgaga gccggccgaa
     1561 cgagggcacg gccaggcgcg attccaggtc ggcgcgcgag cgcacgcggc gatcgagcgt
     1621 ctcgagcagg tagacgatgg cggcggcgag cagcagcccc accaccaccg acaggccggc
     1681 gatcaggccg agctttgggt gcatcggctt gatcggctcg accgccgggg tgaggatcgc
     1741 gacgttggtc tgcttggcgg cgctgtcgac cttgttggtg acgaagcggg cgagcacggc
     1801 gtcgtaggcg cgctgcgcgt tctccacgtc gcgcgtcatc accgccatct cgatgcgggc
     1861 gtccttcatc acctgcacgc gctcggcctg cgccgtgacc gcggtcttca gctcctcctc
     1921 gcggcggcgc gcctgctcga cggtgttgcc caggccggcg acgatgcgct tcatctcggt
     1981 gccgaccttc tcgcgcagcc cggacacctc ggccgaggtg cgctggtatt gtggatggtt
     2041 cggccccagc acctcgccca gctcggcgag gcgcgcctcg gcgcggccga ggtcgacctt
     2101 cagggcgctg atgtgcccgc tggacaggac ctcggggatc gaatcgatcg acgcgccctt
     2161 cagcgtctcg gcggcctgct tctgccggct gatcgcctcg taggtggcgt tcctcg
//
LOCUS       DASYJA010000476         2321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3067983, whole genome shotgun sequence.
ACCESSION   DASYJA010000476 DASYJA010000000
VERSION     DASYJA010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3067983"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VGO02_10350"
     CDS             <1..833
                     /locus_tag="VGO02_10350"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7478123.1"
                     /translation="DFAPDYDGPVCATLVRQPAGEARRGAVLYLHGFVDYFFQRHMAE
                     RFALEGYAFYALDLRKHGRSLRPHQHPNFCKNIAEYYADISRAIEMIGAPVLLAAHST
                     GGLTASLYAHEGERRGEVRALWLNSPFFDWKLPGARRPQLHFAAALGRFFPFIKNPEG
                     MRPEYTQALLEHWDFDTRLKPVEGFPLYFGWIGAINDAHARVQRGLAIGCPVLSMHSD
                     EADWVLDWRDIARWSRGLGRDVAVLAFPGGVHDLVLSRPQIREEVFRQLFAWAERFSE
                     "
     gene            complement(793..1161)
                     /locus_tag="VGO02_10355"
     CDS             complement(793..1161)
                     /locus_tag="VGO02_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HEV7478124.1"
                     /translation="MIADTLLVLHFLIAAFIFGGLLLVWLGAALGWRWVRNPWFRYAH
                     LAAVTFVAAEALLGIACPLTVWEDLLRGSLRAESFVGRWLRRLLYYEAPPWAFTAAYL
                     AWAAATLITLKIVPPRRKAA"
     gene            1186..2223
                     /locus_tag="VGO02_10360"
     CDS             1186..2223
                     /locus_tag="VGO02_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEV7478125.1"
                     /translation="MQSLTAMQTPSQPRVYAGTAGHSAWFSDDLGDTWTHPNSHSGLY
                     TEARVWSLSSHPSDPQSLYAGTDMGIFRWDEATARWELLPSPMQDVWALAQDPDDARV
                     IYAGTRPAGFYRSADGGRTWKALDAPGITTFSEVNKGPTRVTQMLFDPFDRDTMWAGV
                     EIGGIYRSDDRGHSWKLLDKGLVSCDVHGLAVVRNRDGRKSVLATTNKGLHRSEDNGE
                     TWTFQKLDSPWQYTRAITPSADGETVFLTNGNGPPGNSGRLLRSRDAGHTWEEIALPG
                     KLNSSLWCVAVHPADPRLLFACTNLGQVFRSSDGGNTFTRLEREFGEVRALHWRPAAS
                     AGAKHSVWTYK"
BASE COUNT          391 a          808 c          774 g          348 t
ORIGIN      
        1 tagactttgc gcccgactac gacgggccgg tctgcgcgac cctcgtccgc cagcccgcgg
       61 gcgaggcaag gcgcggcgcg gtcttgtacc tccacggctt cgtcgattac ttcttccagc
      121 gccacatggc cgagcgcttc gcgctcgaag gctacgcctt ctacgcgctc gacctgcgca
      181 agcacggacg ttcgctgcgg cctcaccagc atccgaactt ctgcaagaac atcgccgagt
      241 actacgccga catcagccgc gccatcgaga tgatcggcgc gccggtgctg ctcgccgcgc
      301 actccaccgg cggattgaca gcgtcgctgt acgcgcacga gggcgagcgc cgcggcgagg
      361 tgcgtgcgct gtggctgaac agcccgttct tcgactggaa gctgcccggc gcgcgccggc
      421 cgcagctgca cttcgccgcg gcgctcgggc gcttctttcc gttcatcaag aaccccgagg
      481 gcatgcggcc ggagtacacg caggccctgc tcgagcactg ggacttcgac acgcgcctga
      541 agccggtcga gggctttccg ctgtatttcg gctggatcgg cgccatcaac gacgcgcacg
      601 ccagagtgca gcgcggcctc gccatcggct gcccggtgct ctccatgcac tccgacgagg
      661 ccgactgggt cctcgactgg cgcgatatcg cgcgctggtc gcgcggcctc ggccgggacg
      721 tggcggtgct cgccttcccc ggcggcgtgc acgacttggt gctgtcgcgg ccgcagatcc
      781 gcgaggaagt gttcaggcag cttttcgcct gggcggaacg attttcagag tgatcaaggt
      841 cgcggccgcc caggcgaggt aggccgcggt gaaggcccag ggcggggcct cgtagtagag
      901 cagccggcgc agccaccggc cgacgaagga ctcggcccgc aggctgccgc gcagcaggtc
      961 ttcccacacg gtgagcgggc aggcgatgcc gagcagggcc tccgcggcga cgaaagtgac
     1021 cgcggcgagg tgcgcgtagc gaaaccaggg attgcgcacc cagcgccagc cgagcgcggc
     1081 gccgagccag accagcagca ggccgccgaa gatgaaggct gcgatgagga agtggaggac
     1141 gaggagcgtg tcagcgatca ttgcattgac agaaattatc acggtatgca atctcttact
     1201 gccatgcaga ccccctcgca gccgcgtgtt tacgccggca cggccggcca ttccgcctgg
     1261 ttcagcgacg accttggcga tacctggacc catccgaaca gccactcagg tctctatacc
     1321 gaggcacgcg tgtggtcgct ctccagccat ccgtccgatc cgcaatcgct ctatgccggc
     1381 accgacatgg gaatcttccg ctgggacgaa gcgacggcgc gctgggagct gctgccctcg
     1441 ccgatgcagg atgtctgggc gctcgcgcag gaccccgacg acgcccgcgt gatctacgcc
     1501 ggcacgcgcc cggccggctt ctatcgctcg gcggacggcg ggcggacctg gaaagcgctc
     1561 gatgcgccgg ggatcaccac gttctctgaa gtgaacaagg gcccgacgcg cgtcacgcag
     1621 atgctgttcg atccgttcga ccgcgacacg atgtgggccg gagtcgagat cggcggcatc
     1681 tatcgcagcg acgatcgcgg ccacagctgg aaattgctcg acaagggcct ggtgtcgtgc
     1741 gacgtgcacg gcctggccgt ggtgcgcaac cgcgacggcc gcaagtcggt actggccact
     1801 accaacaagg gcctgcaccg cagcgaggac aacggcgaga cctggacctt ccagaagctc
     1861 gattcgccct ggcagtacac gcgcgccatc acgccgagcg ccgacggcga gaccgtattc
     1921 ctcaccaacg gcaacggccc gccgggcaac agcggccgcc tgctgcgcag ccgcgacgcc
     1981 ggccatacgt gggaagagat cgcgctgccg ggcaagctga acagctccct gtggtgcgtc
     2041 gccgtgcacc ccgcggaccc gcgcctgctc ttcgcctgca ccaacctcgg ccaggtgttc
     2101 cgctcgagcg acggcggcaa taccttcacg cgcctcgagc gtgagttcgg cgaggtgcgt
     2161 gcgctgcatt ggcgccccgc agcctcggcc ggggcgaagc attccgtctg gacctacaaa
     2221 taatgaacct gccacgattt cccaagctca gcctggagca gatgacaccc gagcagcgcg
     2281 aggtcgcggc ggagatctcc gccggcccgc gcggcgaggt a
//
LOCUS       DASYJA010000477         3978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1341949, whole genome shotgun sequence.
ACCESSION   DASYJA010000477 DASYJA010000000
VERSION     DASYJA010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341949"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VGO02_10365"
     CDS             <1..733
                     /locus_tag="VGO02_10365"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEV7478126.1"
                     /translation="EVERPVPITEPYKRGEYLLLIDPLDGSSNIDVNISVGTIFSILR
                     CPKLADGKYCEPDEKAFLLAGRSQVAAGFAVYGPTTVFVLTVGDGTHAFTLDRESFTF
                     VLTHPDIRIPPDTQEFAINVSNRRRWEAPVRRYIDECLAGTDGPRGKDFNMRWVASMV
                     ADVFRVLSRGGIFMYPRDAKNKEGRLRLMYEANPMAMIVEQAGGTATDGRTPILDLEP
                     KSLHQRVAVVLGSRNEVEKVASYHS"
     gene            complement(821..1102)
                     /locus_tag="VGO02_10370"
     CDS             complement(821..1102)
                     /locus_tag="VGO02_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HEV7478127.1"
                     /translation="MAKKKAKGKKRKANAAFMKPMTPSATLAAVIGSSAMPRTEVTKK
                     IWGYIKRNSLQDKKNRRMINADDKLKSVFGGKGQVSMFEMTKLVSRHLK"
     gene            complement(1235..2446)
                     /gene="egtB"
                     /locus_tag="VGO02_10375"
     CDS             complement(1235..2446)
                     /gene="egtB"
                     /locus_tag="VGO02_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEV7478128.1"
                     /translation="MPAILPAEHRDPAQRYAAVRAQTAALADPLSAEDCALQSMPEAS
                     PVKWHLGHTSWFFETFVLGGEPWRPEFRVLFNSYYLGIGERHPRPERGLLSRPSLEEV
                     RAYRASVDERMQALFRSAAWRERAALIELGLNHEQQHQELILTDVKHLFSRNPLAPSY
                     GKRSFSTGNSTPMGWRQFPAGVREIGHDGAGFAFDNEAPRHRVFADEFGIADRLVTNG
                     EYRAFIDDGGYRRPELWLSEGWDLCRAQGWSAPLYWRDGAEFTLHGLRAIEPAQPVVH
                     VSYFEADAYARWAQARLPTEAEWEIAAQGRPVEGNLLDSGRLHPAGGPSPAFGDAWQW
                     TSSAYAPYPGFRAASGAVGEYNGKFMVNQYVLRGGSCATPASHVRVSYRNFFPAAARW
                     QFSGVRLARNA"
     gene            2445..3077
                     /gene="pdxH"
                     /locus_tag="VGO02_10380"
     CDS             2445..3077
                     /gene="pdxH"
                     /locus_tag="VGO02_10380"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEV7478129.1"
                     /translation="MKLADLRQEYMRAGLSEAEVDRDPLRQFERWFDDALRARLPLAN
                     AMTLATVTPQGAPSARIVLLKGVEDGGFVFYTNYLSRKGRELSARPVACLVFQWSDLE
                     RQVRIDGAVEQVSAAASDAYYATRPAGARLSAWASAQSEKVASRKVLETAMQAVRSRY
                     ADDPPRPPHWGGYRVVPQAIEFWQGRADRLHDRLLYTRHAAGWTIERLSP"
     gene            3058..>3978
                     /locus_tag="VGO02_10385"
     CDS             3058..>3978
                     /locus_tag="VGO02_10385"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV7478130.1"
                     /translation="MSASLPEPVLQFLREAHLAAPGESPSAVALSGGVSSDIWRVDLA
                     SGTLCVKGALPRLRVSQLWEAPVERNRYEYEWLRVAGRAAPGAAPRVYAEQAGLFAME
                     YLDFPVWKSLLREGRAEPAFAAKIGAALGAIHAATAGREEIARSFPTDAIFYAIRLEP
                     YLVATAAKHPDLRTQFERLVERTANTKLCLVHGDVSPKNILAAPHGPVFLDAECAWYG
                     DPAFDLAFCLNHLLLKTLWVPSAALRFLQCFDSLKDAYLKSVNWEAPGGIEERTASLL
                     PGLFLARVDGKSPVEYLTEESDKEKVRNFAR"
BASE COUNT          639 a         1366 c         1285 g          688 t
ORIGIN      
        1 agaggtggag cgcccggtgc cgatcaccga gccgtacaag cgcggcgaat acctgctgct
       61 gatcgacccg ctcgacggct catcgaacat cgacgtcaac atctcggtgg gcaccatctt
      121 ttcgatcctg cgctgcccca agctggccga cggcaagtac tgcgagcccg acgagaaggc
      181 cttcctgctg gccggccgca gccaggtcgc ggccggcttc gcggtctacg ggccgaccac
      241 ggtcttcgtg ctcacggtcg gcgacggcac gcacgccttc accctcgacc gcgagagctt
      301 caccttcgtc ctcacccacc ccgacatccg catcccgccc gacacgcagg agttcgccat
      361 caacgtgtcg aaccggcggc gctgggaggc gccggtgcgg cgctacatcg acgagtgtct
      421 cgccggcacc gacgggccgc gcggcaagga cttcaacatg cgctgggtgg cttcgatggt
      481 ggccgacgtg ttccgcgtgc tctcgcgcgg cggcatcttc atgtacccgc gcgacgcgaa
      541 gaacaaggaa gggcgcctgc gcctgatgta cgaggccaac ccgatggcga tgatcgtcga
      601 gcaggccggc ggcacggcga ccgacgggcg caccccgatc ctcgacctcg agccgaagtc
      661 cctgcaccag cgggtggcgg tggttctcgg ctcgcgcaac gaggtcgaga aggtcgcctc
      721 gtaccactcg taaccagcgc gtaaccagcg cgtaaaacgc cctggaaaag aaaaggccgg
      781 gtcggacccg gcctttccaa ctgctccctg ttgaatcggg ttacttcagg tgccggctga
      841 ccagtttggt catctcgaac atcgagacct gacccttgcc gccgaaaacc gacttcagct
      901 tgtcgtcggc gttgatcatg cgacggttct tcttgtcctg gaggctgttg cgcttgatgt
      961 agccccagat cttcttggtg acttcggtgc ggggcatcgc gctggagccg atcaccgccg
     1021 cgagcgtcgc ggacggggtc atcggcttca tgaatgctgc attggcctta cgcttcttgc
     1081 ccttcgcctt tttcttcgcc atctcgattt ctcctctgaa cgttcggact tctatccgga
     1141 ttttttcccg ccgcctgcgc tgcgagagac gtgacgatgc cgaatcctgc ggggcgtgtc
     1201 aatgcttttc agagggaaaa cgtgcttctt tccctcaggc gttgcgcgcc aaccgcacgc
     1261 cgctgaactg ccagcgcgcc gcagcgggaa agaaattgcg gtagctgacg cgcacgtgcg
     1321 aggcaggcgt ggcgcacgat ccgcctcgaa gaacatactg gttcaccatg aacttgccgt
     1381 tatattctcc gacggcgccc gaagccgcgc ggaagcccgg atacggtgcg tacgcgctcg
     1441 aggtccactg ccatgcatcg ccgaaggcgg gcgacgggcc gcccgccgga tgcagcctgc
     1501 cgctgtcgag cagattgccc tctaccggcc ggccctgcgc ggcgatttcc cactccgcct
     1561 cggtgggcag ccgcgcttgc gcccagcgcg cgtaggcgtc cgcctcgaag tagctgacgt
     1621 gcacgacggg ttgcgccggc tcgatggcgc gcaggccgtg cagcgtgaat tccgcgccgt
     1681 cgcgccagta gagcggcgcg ctccatcctt gtgcacggca cagatcccag ccctccgata
     1741 gccacagctc gggccgccgg tagccaccgt cgtcgatgaa tgcgcggtat tcgccattgg
     1801 tcaccaggcg gtccgcgatg ccaaactcat cggcaaatac ccgatgacga ggcgcttcgt
     1861 tgtcgaacgc aaagcccgcc ccgtcatgcc cgatctcgcg cacgcctgcc gggaactgcc
     1921 gccatcccat gggcgttgaa tttccagtgg aaaagcttct tttcccatag gagggggcga
     1981 gcggatttct cgagaagaga tgcttcacgt cggtgaggat cagctcctgg tgctgctgct
     2041 cgtggttgag gccgagctca atcagcgcag cgcgctcgcg ccatgccgcg ctgcgaaata
     2101 acgcctgcat ccgctcgtcg acgcttgccc ggtaggcgcg tacctcctct agagagggac
     2161 gcgaaaggag gccgcgctcc gggcgcgggt gacgctcgcc gattcccagg tagtaggaat
     2221 tgaacagaac ccgaaactcc ggcctccacg gctcgccgcc gaggacgaac gtctcgaaga
     2281 accagctggt gtggccgagg tgccacttga ccgggcttgc ctcgggcatc gattgcaggg
     2341 cgcagtcctc ggcgctcagc ggatcagcga gcgccgcggt ctgcgcccgc acggcggcat
     2401 agcgctgggc ggggtcacgg tgctcggcgg gtagaatcgc tggcatgaaa ttggcagacc
     2461 tgcgacaaga atacatgcgc gcgggccttt ccgaggcaga agtcgatcgc gacccgttgc
     2521 gccagttcga gcgctggttc gacgacgcgc tgcgcgccag gctgcccctg gccaacgcca
     2581 tgaccctggc gacggtcacc ccgcagggcg cgccttccgc ccgcatcgtg cttttgaagg
     2641 gggtcgagga cggcggcttc gtcttctaca ccaactacct cagccgcaag ggccgcgagt
     2701 tgtcggcccg gcccgtcgcc tgcctagtct tccagtggtc cgacctcgag cgtcaggtgc
     2761 gcatcgacgg cgccgtggag caggtgtctg cggctgcctc cgacgcgtac tacgccaccc
     2821 gccccgccgg cgcgcggctt tcggcctggg cctcggcgca gagcgagaag gtggcttcgc
     2881 gcaaggtcct ggaaaccgcc atgcaggcgg ttcgcagccg ctatgccgac gacccgccgc
     2941 gcccgccgca ctggggcggc tatcgcgtcg tgccgcaggc gatcgagttc tggcagggcc
     3001 gcgccgaccg cctgcacgac cgcctgctct acacgcgcca cgcggccggc tggaccattg
     3061 agcgcctctc tccctgaacc ggtcctgcag ttcctgcgcg aggcgcatct cgccgcgccg
     3121 ggggaatcgc cttcagccgt cgcgctgtcc ggcggcgtct cctcggacat ctggcgggtg
     3181 gacctggcga gcgggacgct gtgcgtcaag ggcgcgctgc ccaggctgcg ggtgagccag
     3241 ctctgggagg cgcccgtcga gcgcaaccgc tacgagtacg aatggctgcg cgtggccggc
     3301 cgcgccgcgc ccggcgcggc gccgcgcgtg tatgccgagc aggcgggcct gttcgccatg
     3361 gagtatctcg atttcccggt gtggaagtcg ctgctgcgcg aagggcgcgc cgagccggcc
     3421 ttcgccgcca agataggcgc cgcgctcggc gccatccacg cggccaccgc cgggcgcgag
     3481 gaaatcgcgc gcagctttcc gaccgatgcc attttctacg ccatccggct cgaaccatat
     3541 ctggtcgcca ccgccgcaaa gcatcctgac ctccggacgc aatttgagcg cctggtggaa
     3601 agaacggcga acacgaagct ctgtctggtg catggcgacg tcagcccgaa gaacatcctc
     3661 gccgcgccgc acggcccggt gttcctcgac gccgagtgcg cctggtatgg cgacccggct
     3721 ttcgacctcg ccttctgcct caatcatctt ctcctcaaaa ctctctgggt cccttcggcg
     3781 gccctgcggt tcctccagtg tttcgattca ctgaaagacg cctatctgaa gagcgtgaac
     3841 tgggaagcgc ccggagggat cgaggagcgg accgcaagtc ttctgcccgg cctgttcctc
     3901 gcgcgcgtcg acggcaagtc gccggtggag tacctcacgg aggaatccga caaggaaaag
     3961 gtgaggaatt tcgcccgg
//
LOCUS       DASYJA010000478         6590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2977175, whole genome shotgun sequence.
ACCESSION   DASYJA010000478 DASYJA010000000
VERSION     DASYJA010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6590
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2977175"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2070
                     /gene="glnE"
                     /locus_tag="VGO02_10390"
     CDS             <1..2070
                     /gene="glnE"
                     /locus_tag="VGO02_10390"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEV7478131.1"
                     /translation="LVRLLSETTEDGFTFRVDMRLRPYGDSGPLVCNLEFLETYFISQ
                     GREWERYAWIKARALTGSLATELDHVVRPFVFRKYLDYASLGAMRRLHAEVRREVERR
                     ELAGNVKLGPGGIREIEFVAQALQLVRGGRDAELTARPTLQVLSFLSRKNLLPEKAAQ
                     ELSEAYVFLRNVEHRLQYLDDAQRHDLPEEGEDRGLLAGMCGFASWISFAEKLDSHRA
                     AVTRHFQAVFAEDKQQLEAWPEHPRLATLRASQRYAALPEDSRRRLDAVVPALARAAA
                     ATPRPQETIARGADLIEAIASRAAYLALLAENPPALARVARIIGASSWAAGFVTRHPL
                     LLDELLDDRVLYAPIELESFSKNLRAQLAAHGGDAERQMNDLREMHQAQVFRLLAQDL
                     AGLLTVEKLADHLSALADLVLNQTLHLVWQDLRTRHREHPAFAVIGYGKHGGKELGYA
                     SDLDIIFLYDDPDERAPEVYARLAQRLNLWLTTHTSAGVLFDTDLRLRPSGASGLMVS
                     SIEAFERYQEKDAWVWEHQALTRARYCAGDAAVGGKFEAIRQSILKRKRDVATLRDEI
                     AEMREKMHAAHPNKSGLFDVKHDRGGMIDIEFAVQFLVLEFSAQFPELTGNLGNIALL
                     GMSAQRGLIPADLAERCREAYREFRRIQHALRLEGAKYARVPPEQVRAQAEAVRELWR
                     ASVAPSA"
     gene            complement(2048..2473)
                     /locus_tag="VGO02_10395"
     CDS             complement(2048..2473)
                     /locus_tag="VGO02_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEV7478132.1"
                     /translation="MDKFRFSDFARRLAELAGRPATFLGAVLLIVLWALAGPFFGYSD
                     SWQLVVNTTTTIITFLMVFLIQNTQNRDTAAIQLKLDELIRATQGAHNALLDLEEIDD
                     ERFEQYRASYRRLAAEAREKLKSGELDTDSPEIKHSAPR"
     gene            complement(2501..3286)
                     /locus_tag="VGO02_10400"
     CDS             complement(2501..3286)
                     /locus_tag="VGO02_10400"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HEV7478133.1"
                     /translation="MLRSITIACLLLAGCGALAPVDELTWSSELAKSLPLQAGSVIEV
                     ARFSRLRPGAFYNMGEWEPFIVVEGSTPTLYRPVERDGVVVIEADSGEGSSALYRKIH
                     INPHSHPIIEWRWRVPRESGAGGEGDPSSVSPPVRLSLAFDGDPAKLDFDDRTKLRLA
                     KALTVNGLPYASLLYVWKNHDPVETVYASPHTERVRHIVVESGERRLDQWVSMRRNVL
                     EDYRRVFGEEPGDIVAVGIMTDYGDNAAPRRALYGDITFRSAP"
     gene            complement(3307..3612)
                     /locus_tag="VGO02_10405"
     CDS             complement(3307..3612)
                     /locus_tag="VGO02_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV7478134.1"
                     /translation="MIVNPVEGVFEALASLPRRRMLSFLSQTALSTVELAERFGMSAP
                     AVSRHLAVLQQAGLVSGEREGQRVLYRLHRDVLLGALARLASEVDGPLRRAPPREAI"
     gene            3710..4627
                     /locus_tag="VGO02_10410"
     CDS             3710..4627
                     /locus_tag="VGO02_10410"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEV7478135.1"
                     /translation="MADRDGWIWYDGKLVPWRSATTHVLTHSLHYGLAVFEGVRAYKT
                     AIGTAIYRLKEHTDRLFNSAHIYMMKIPFTREQLMKAQVEVVKANKHEACYLRPIAFY
                     GSEKMGVSPVGAKVHVAIAAWPWGAYLGPDALAKGIRVKTSSYARHHVNVTMARAKMS
                     GTYPNSVLATLEATQHGYDEGLLLDVDGFVAEGAGENIFMVKDKVIYEPELTSALTGI
                     TRSSVIELAAELGYQVISKRLTRDDIYIADECFFTGTAAEVTPIRELDGRVIGAGKAG
                     PITKRIQDAFFAVVTGKDKKRRTWLTPVK"
     gene            4606..4791
                     /locus_tag="VGO02_10415"
     CDS             4606..4791
                     /locus_tag="VGO02_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEV7478136.1"
                     /translation="MADPRQVEVTAKDLPLHCPLPGAPLWSRHPRVFLDVAHEGEVLC
                     PYCGTKYVYSGAPVKGH"
     gene            4796..5752
                     /gene="waaF"
                     /locus_tag="VGO02_10420"
     CDS             4796..5752
                     /gene="waaF"
                     /locus_tag="VGO02_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011401829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEV7478137.1"
                     /translation="MPRLLVVAPNWIGDALMAQPLLARLHEKLAGLRIEVLAPPWVAP
                     VARRMPEVAEVIEAPLRHGPLQLRSRWRLGRGLRQRRYDQAIVLPNTWKSALVPFFAD
                     IPLRSGYVGESRYGLLNLLYRNSAAAMRLHYARLAEPPGTEPAEPLPRPRLRVDSHEI
                     ELVKQRFGIVGRYAVLCPGAEYGPAKRWPYFAELAASMSVPVVVLGSPADLEPARGIQ
                     GTNLVGKTSLDEAISLIAGAQAVVSNDSGLMHVAAALGRPQVALYGSSSPEHTPPASA
                     AARVLWLHIECSPCFQRECPLGHFRCMKEISVDHVLAELQKL"
     gene            5749..6198
                     /locus_tag="VGO02_10425"
     CDS             5749..6198
                     /locus_tag="VGO02_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7478138.1"
                     /translation="MSNKIFPTAQDAENAFYEALERCDLEGMMAVWAEDDDIVCVHPA
                     GPRLTGQDQIRESWARIFSAGARARVHITNQVAISGMMLAVHSVHENFTVGQPRGDAA
                     AQPVPVVATNIYLRTAAGWRMIVHHASPAPAATQKPAPRETPPKLLH"
     gene            6195..>6590
                     /locus_tag="VGO02_10430"
     CDS             6195..>6590
                     /locus_tag="VGO02_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7478139.1"
                     /translation="MIGHWQTVSAALFPPPAVRFQRSRWETPDGDFIDVDFAGAANAR
                     PMVLLHGLEGCSDSHYARLLAGVLPAAGWRVAIPHWRGCSGEANRKPRAYHSGDTAEL
                     DWILRRFKHPVDVIGVSLGGNVLLKWLAER"
BASE COUNT         1052 a         2263 c         2243 g         1032 t
ORIGIN      
        1 ctcgttcgcc tgctctctga gaccaccgag gacggcttta cgttccgtgt tgacatgcgg
       61 ctgcgcccct acggcgattc gggcccgctc gtgtgcaacc tcgagttcct ggagacttat
      121 ttcatctccc agggccgcga atgggagcgc tacgcctgga tcaaggcgcg cgcgctgact
      181 ggcagcctcg cgaccgagct cgaccatgtg gtgcgcccgt tcgtcttccg caagtacctc
      241 gactacgcga gcctcggcgc gatgcgccgc ctgcacgccg aggtgcgccg cgaggtcgag
      301 cgccgcgagc tcgccggcaa cgtcaagctc ggccccggcg gcatccgcga gatcgagttc
      361 gtggcgcagg cgctgcagct cgtgcgcggc gggcgcgatg ccgagctcac cgcgaggccg
      421 acgctgcagg ttctgtcttt tctttcacgc aagaacctgc tgccggaaaa agccgcgcaa
      481 gagctcagcg aagcttatgt gttcctgaga aatgtcgagc accggctgca atacctcgac
      541 gatgcgcagc gccacgacct gcccgaggaa ggcgaggacc gcgggctgct cgccggcatg
      601 tgcggctttg catcatggat ctcctttgcc gaaaaactcg acagccaccg cgccgcggtg
      661 acccggcatt tccaggcggt gttcgccgag gacaagcagc agctcgaagc ctggcccgag
      721 catccgcgcc tcgccacgct gcgcgcgagt cagcgctacg cggcgctgcc cgaggattca
      781 cgccgccgcc tggacgcggt cgtcccggcg ctggcgcgcg ccgcggccgc cacgccgcgg
      841 ccgcaggaga ccatcgcccg cggcgccgac ctgatcgagg cgatcgcgag ccgcgccgcc
      901 tacctcgcgc tgctcgccga gaacccgccg gcgctggcgc gggtcgcgcg catcatcggc
      961 gcgtcgagct gggccgccgg gttcgtcacg cgccatccgc tgctgctcga cgagctgctc
     1021 gacgaccggg tgctgtacgc gccgatcgag ctcgagtctt tttcgaagaa tctgcgcgcc
     1081 cagctcgccg cgcacggcgg cgacgcggag cggcagatga acgacctgcg cgagatgcac
     1141 caggcgcagg tgttccgcct gctggcgcag gacctcgccg ggctgctcac ggtcgagaag
     1201 ctggccgacc atctctccgc cctggccgac ctggtgctga accaaaccct gcatctggtg
     1261 tggcaggacc tgcgcaccag gcatcgcgag catccggcct tcgcggtgat cggctacggc
     1321 aagcacggcg gcaaggagct cggctatgcc tccgacctcg acatcatttt cctgtacgac
     1381 gaccccgacg agcgcgcgcc cgaggtctat gcgcgcctcg cgcagcgcct gaacctctgg
     1441 ctcaccaccc acacctcggc cggcgtgctc ttcgataccg acctgcggct gcgcccgagc
     1501 ggcgcctccg ggctgatggt gtcgtcgatc gaggctttcg agcgctacca ggagaaggat
     1561 gcctgggtgt gggaacacca ggcgctgacg cgcgcgcgct actgcgcagg ggacgcggcg
     1621 gtaggcggta agtttgaagc gatacgacag agcatcttga aaagaaagcg ggacgtagca
     1681 accctgcgcg acgaaatcgc tgaaatgcgc gagaagatgc acgccgccca tcccaacaaa
     1741 tccggtctgt tcgacgtcaa gcacgaccgc ggcggcatga tcgacatcga gttcgcagtc
     1801 cagtttctgg ttttggaatt ctcggcgcaa ttccccgagc tgaccggcaa tctcggcaac
     1861 atcgcgctgc tgggaatgag cgcgcagcgt ggcttgattc ccgccgacct cgccgagcgc
     1921 tgccgcgagg cctaccgcga gtttcgccgc atccagcacg cgctgcgcct ggagggcgcg
     1981 aagtacgcgc gcgtgccgcc cgagcaggta cgcgcgcagg cggaggcggt gcgcgagctg
     2041 tggcgcgcta gcgtggcgcc gagtgcttga tttccggcga gtcggtgtcg agctcgccgc
     2101 tcttcagctt ctcgcgcgcc tcggcggcga gccggcggta gctcgcgcgg tactgctcga
     2161 agcgctcgtc gtcgatctct tccaggtcga gcagcgcgtt gtgcgcgccc tgcgtggcgc
     2221 ggatcagctc gtcgagcttg agctggatcg ccgcggtgtc gcggttctgc gtgttctgga
     2281 tcaggaacac catcaggaag gtgattatgg tggtggtggt gttgaccacc agctgccagg
     2341 agtcgctgta gccgaagaac gggcccgcca gggcccacag aacgatcagc agcaccgcgc
     2401 cgaggaatgt cgccggccgg ccggcgagct ccgccagccg tcgcgcgaag tcggaaaatc
     2461 tgaatttatc catggtgggt ccttgtgggc ggctggcgga ctacggcgcg ctgcggaacg
     2521 tgatgtcgcc gtagagggcg cggcgcggcg cggcgttgtc gccgtagtcg gtcatgatac
     2581 cgaccgcgac gatgtcgccc ggttcctcgc cgaacacgcg ccggtagtcc tccaggacgt
     2641 tgcggcgcat gctgacccac tggtcgagcc ggcgctcgcc gctttccacc acgatgtggc
     2701 gcacgcgctc ggtgtgcggg ctggcgtaaa ccgtctccac cgggtcatgg ttcttccaga
     2761 cgtagagcag cgacgcgtac ggcaggccgt tgacggtgag cgccttggcc aggcgcagct
     2821 tggtgcggtc gtcgaagtcg agcttcgccg ggtcgccgtc gaaggcgagc gacaggcgca
     2881 ccggcgggct gacgctcgac gggtcgcctt cgccgccggc gccggactcc cgcggcacgc
     2941 gccagcgcca ctcgatgatc ggatggctgt gcggattgat gtggatcttg cgatagaggg
     3001 ccgagctgcc ctcgcctgaa tcggcctcga tcaccaccac gccgtcgcgc tcgaccgggc
     3061 ggtagagggt cggcgtgctg ccctccacca cgatgaacgg ctcccattcg cccatgttgt
     3121 agaaggcgcc ggggcgcagc cgcgagaagc gcgccacctc gatcacgctg cccgcctgca
     3181 gcggcagcga cttcgccagc tcgctcgacc aggtcagctc gtcgaccggg gcgagcgcgc
     3241 cgcagccggc gagcagcagg catgcgatgg tgatcgaccg gagcatgtgg aatatcctaa
     3301 acaattctag atcgcctcgc gcggcggcgc ccggcgcagg ggtccgtcga cctcggaggc
     3361 gaggcgcgcc agggcgccga gcaggacatc gcggtgcagc cggtaaagga cgcgctggcc
     3421 ttcgcgttcg cccgagacga gcccagcctg ctgcagcacg gcgagatggc gcgagaccgc
     3481 gggcgcgctc atgccgaagc gctccgccag ctcgacggtc gacaacgcgg tctgcgacag
     3541 gaacgacagc atgcgccggc gcggcaggga ggccagcgct tcgaacacgc cctccacagg
     3601 attaacaatc atgttaatca ttatggcatg gcgggccggg ctgtgcttag aatgcgcact
     3661 cgatcccaag gaggcagatt cgatggcgaa gaagcaggcg ccgctttcga tggcggaccg
     3721 cgacggctgg atctggtacg acggcaagct ggtgccgtgg cgcagcgcca ccacgcacgt
     3781 cctcacccat tcgctgcact acggcctggc ggtgttcgag ggcgtgcgcg cctacaagac
     3841 ggcgatcggc accgcgatct accggctgaa ggagcacacc gaccgcctgt tcaactcggc
     3901 gcacatctac atgatgaaga tcccgttcac gcgggagcag ctgatgaagg cgcaggtcga
     3961 ggtggtgaag gcgaacaagc acgaggcctg ctatctgcgg ccgatcgcgt tctacggctc
     4021 ggagaagatg ggcgtgtcgc cggtcggcgc gaaggtgcac gtcgccatcg ccgcgtggcc
     4081 ctggggcgcc tacctcgggc ccgacgcgct cgccaagggc atccgcgtca agacctcgtc
     4141 ctacgctcgc caccacgtca acgtgacgat ggcgcgcgcc aagatgtcgg gcacctaccc
     4201 caactcggtt ctggcgacgc tcgaggcgac gcagcacggc tatgatgagg ggctgctgct
     4261 cgacgtcgac ggcttcgtgg cggaaggtgc gggtgaaaat attttcatgg taaaggataa
     4321 agtcatctat gagcccgagc tcacttcggc gctcaccggc atcacccgca gctccgtcat
     4381 cgagctcgcc gcggagctcg gctaccaggt gatctccaag cgcctgacgc gcgacgacat
     4441 ctacatcgcc gacgagtgct tcttcaccgg caccgccgcc gaggtgacgc cgatccgcga
     4501 gctcgacggc cgcgtcatcg gcgccggcaa ggccgggccg atcacgaaga ggatccagga
     4561 cgcatttttc gccgtcgtca ccggcaagga caagaaacgg cgcacatggc tgacccccgt
     4621 caagtagaag tcacggcgaa ggacctgccg ctgcactgcc cgctgcctgg cgcgccgctc
     4681 tggtcgcgcc acccgcgcgt gttcctggat gtcgcgcacg aaggcgaggt cctctgcccg
     4741 tactgcggca cgaagtacgt ctacagcgga gctccagtca aggggcacta ggcttttgcc
     4801 gcgcctcctc gtcgtcgcgc ccaactggat cggtgacgcg ctgatggcgc agccgctgct
     4861 cgcgcggctg cacgagaagc tcgccggcct gcgcatcgag gtgctcgcgc cgccgtgggt
     4921 ggcgccggtg gcgcggcgca tgcccgaggt cgccgaggta atcgaggcgc cgctgcgcca
     4981 cggcccgctg cagttgcgca gccgctggcg cctcggccgc ggcctcaggc agcgccgcta
     5041 cgaccaggcg atcgtgctgc ccaacacctg gaaatccgcg ctggtgccgt tcttcgccga
     5101 catcccgctg cgcagcggct atgtgggcga atcccgctac gggctgctga atctgctcta
     5161 caggaacagc gccgcggcca tgcgcctgca ctatgcgcgc ctggccgagc cccccgggac
     5221 ggagccggcc gagccgctgc cgcgcccgcg cctgcgggtc gattcgcatg aaatcgaact
     5281 ggtgaaacag cgcttcggca tcgtcggccg ctatgccgtc ctgtgtccgg gcgccgagta
     5341 cgggccggcc aagcgctggc cctactttgc cgagctcgcc gcaagcatga gcgtcccggt
     5401 cgtggtgctc ggctcgccgg ccgacctcga gccggcgcgc ggcatccagg gcaccaacct
     5461 ggtgggcaag accagcctgg acgaggcgat ctcgctgatc gcgggggcgc aggcggtggt
     5521 gagcaacgac tcaggcctga tgcacgtcgc cgccgcgctc ggccggccgc aggtggcgct
     5581 gtacggctcg tcgagccccg agcacacgcc gccggcgagt gccgcggcgc gcgtgctgtg
     5641 gctgcacatc gagtgcagcc cgtgcttcca gcgggagtgt cctttggggc attttcgttg
     5701 tatgaaggag atctccgtcg atcacgtcct ggccgaactg cagaaactgt gagcaacaag
     5761 atctttccca ccgcgcagga tgcggagaac gcgttctacg aggccctcga gcgctgcgac
     5821 ctcgaaggca tgatggcggt gtgggccgag gacgatgaca tcgtctgcgt ccatcccgcc
     5881 ggcccgcgcc tcaccgggca ggaccagatc cgcgagagct gggcgcgcat cttctccgca
     5941 ggagcgcgcg cacgcgtgca catcacgaac caggtggcga tcagcggcat gatgctcgcg
     6001 gtgcacagcg tgcacgagaa tttcacggtg gggcagcccc ggggtgatgc tgcggcgcag
     6061 ccggtgccgg tggtcgcgac caacatctat ctgcgcaccg cggcgggctg gcgcatgatc
     6121 gtgcaccacg cctcgcccgc gcccgcggcc acgcaaaagc ccgcgccgcg cgaaacgccg
     6181 cccaagctcc tgcattgatc ggacactggc agaccgtctc ggcggcgctt tttccaccgc
     6241 cggccgtgcg ctttcagcgc agccgctggg aaacgcccga cggtgatttc atcgatgtcg
     6301 acttcgccgg cgccgccaac gcgcgcccga tggtgctgct gcacggcctg gaaggctgct
     6361 ccgacagcca ttacgcgcgc ctgctcgcgg gcgtgctgcc cgcggcgggc tggcgcgtcg
     6421 cgattccaca ctggcgcggc tgctcgggcg aagccaaccg caagccacgc gcctatcact
     6481 cgggcgacac cgccgagctc gactggatcc tgcggcgctt caagcatccg gtggatgtga
     6541 tcggcgtctc gctcggcggc aacgtgctcc tcaagtggct cgccgagcgc
//
LOCUS       DASYJA010000479         2359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1889177, whole genome shotgun sequence.
ACCESSION   DASYJA010000479 DASYJA010000000
VERSION     DASYJA010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889177"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VGO02_10435"
     CDS             <1..348
                     /locus_tag="VGO02_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HEV7478140.1"
                     /translation="FYACPFGAPQFPQAGAFGLRGKMDKCTFCAGGPEADRSDAEFKK
                     YGRNRLAEGKLPACAEMCSTKALLGGDGDVIADIYRERVLRRGKGSEVWGWGTAYGPG
                     KPGAQPPAPARKS"
     gene            345..602
                     /locus_tag="VGO02_10440"
     CDS             345..602
                     /locus_tag="VGO02_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478141.1"
                     /translation="MRRVFACLTIFSVVYGISGCVEREQVVEQQSEKRYQGKRDTQPW
                     DNAPLAAEFRGGTWTKGDERSWEDQIKERQLGQNEYKRIYQ"
     gene            631..1707
                     /locus_tag="VGO02_10445"
     CDS             631..1707
                     /locus_tag="VGO02_10445"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEV7478142.1"
                     /translation="MRMLFLALSFALAGAAGAQQSPPKDDPAPTPQQTQQERQVTQPG
                     NNAPVWREVQSGQPNYTSIPGRETGVLIQPQARFPGQEHRSTAGEAWRQFRNGPMTFY
                     GGWLVLLVLIAIAALYFSKGPVRLHEGLSGRKILRFTLSERVIHWTVAISFCILGLSG
                     LLMLFGKYVLLPIIGYTLFGWLTALGKNLHNFVAPFFIVSVLAMIVVYIRDNFPKWYD
                     LRWFGKAWAFFARGEEVPSGRFNAGEKAWFWIGVIGLSIVVSCSGVILLFPNFDQSRA
                     VMQDAWIWHAGAALLYIAAALGHIYMGTIGVEHTYENMREGYADENWAKEHHRYWYEE
                     VKSGKREGAGGALPAGAPHMKEKS"
     gene            1704..1946
                     /locus_tag="VGO02_10450"
     CDS             1704..1946
                     /locus_tag="VGO02_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478143.1"
                     /translation="MKSILAAVAAGLMIGVFAASHAKLPASPPKSDAEKAADADKAAA
                     AKAKDAADNAKAQDKAVANYKKNKGIAMSPTKPAKK"
     gene            1943..>2359
                     /locus_tag="VGO02_10455"
     CDS             1943..>2359
                     /locus_tag="VGO02_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEV7478144.1"
                     /translation="MKHWALPLAAVALLAGCESAKGMWSNLTSSGAKATAELEPTKGS
                     SVRGTVSFVQAADRVRVSGAVSGLQPDSEVGFHIHEAGDCSSGDGMSAKGHFNPEGKP
                     HGHPASRERHAGDMPMLRADASGNASISADLDIITVA"
BASE COUNT          416 a          771 c          832 g          340 t
ORIGIN      
        1 ttctacgcct gcccgttcgg cgcgccgcag ttcccgcagg ccggcgcctt cggcctgcgc
       61 ggcaagatgg acaagtgcac cttctgcgcc ggcggccccg aggccgaccg cagcgatgcc
      121 gagttcaaga agtacggccg caaccgcctc gccgagggca agctgcccgc gtgcgccgag
      181 atgtgctcga ccaaggcgct gctcggcggc gacggcgatg tcatcgccga catctaccgc
      241 gagcgcgtgt tgcgccgcgg caagggctcg gaagtctggg gctggggcac ggcctacggc
      301 ccgggcaagc ccggcgcgca accgcctgcg ccggcgagga agtcatgagg cgggttttcg
      361 cttgcctcac gattttctct gttgtttacg gaatatcagg ctgtgtcgag cgcgagcagg
      421 tcgtcgagca gcagtccgag aagcgctacc agggcaagcg cgacacccag ccctgggaca
      481 acgcgccgct ggcggcggag ttccgcggcg gcacgtggac caagggcgac gagcgcagct
      541 gggaggacca gatcaaggag cgccagctcg gccagaacga gtacaaacgc atctaccaat
      601 aggcagagcg gccctcgaaa ggagccttgg atgaggatgc tttttctggc gctgtcgttc
      661 gccctggcgg gcgcggccgg cgcgcagcaa tcgccgccga aggacgatcc ggcgccgacc
      721 ccgcagcaga cgcagcagga gcggcaggtc acccagccgg gaaacaacgc cccggtatgg
      781 cgcgaggtgc agtcgggcca gcccaactac accagcattc ccggccgcga gaccggcgtg
      841 ctgatccagc cgcaggcgcg cttcccgggc caggagcacc gctcgaccgc gggcgaggcg
      901 tggcgccagt tccgcaacgg gccgatgacc ttctacggcg gctggctggt gctgctggtg
      961 ctgatcgcca tcgccgccct gtacttttcg aaggggccgg tcaggctgca cgaggggctg
     1021 agcgggcgca agatcctgcg cttcacgctg agcgagcggg tcatccactg gacggtggcg
     1081 atcagcttct gcatcctggg gctgtccggg ctgctgatgc tgttcggcaa gtacgtgctg
     1141 ctgccgatca tcggctacac gctgttcggc tggctgaccg cgctgggcaa gaacctgcac
     1201 aacttcgtag cgccgttctt catcgtgtcg gtgctggcga tgatcgtggt gtacatccgc
     1261 gacaatttcc cgaagtggta cgacctgcgc tggttcggca aggcctgggc gttcttcgcc
     1321 cgcggcgagg aagtgccctc cgggcgcttc aacgccggcg agaaggcctg gttctggatc
     1381 ggcgtgatcg gcctgagcat cgtggtgagc tgcagcggcg tcatcctgct gttcccgaac
     1441 ttcgaccagt cgcgcgcggt gatgcaggac gcctggatct ggcatgcggg cgcggccctt
     1501 ctctatatcg cggccgcgct cggccacatc tatatgggaa ccatcggcgt cgagcacacc
     1561 tacgagaaca tgcgcgaggg ctacgccgac gagaactggg ccaaggagca ccaccggtac
     1621 tggtacgagg aagtgaagtc cgggaagcgc gagggcgcgg gtggcgcgct gcccgcgggt
     1681 gcgccccata tgaaggagaa atcatgaaat cgatactggc cgcggttgcc gcggggctga
     1741 tgatcggcgt gtttgcagcc tcccacgcca agctgccggc gtcgccgccg aagagcgatg
     1801 ccgagaaggc cgccgacgcc gacaaggccg ccgccgcgaa ggcgaaggac gccgccgaca
     1861 acgccaaggc gcaggacaag gcggtggcca actacaagaa aaacaagggc atcgccatga
     1921 gcccgaccaa gccggcgaag aaatgaagca ctgggccctt ccgctggccg ctgtggcgct
     1981 gctggcgggg tgcgagagcg cgaagggcat gtggtcgaac ctgacctcga gcggggcgaa
     2041 agccaccgcc gagctcgagc cgaccaaagg cagcagcgtg cgcggcacgg tgagcttcgt
     2101 gcaggccgcc gaccgtgtgc gcgtgagcgg cgcggtctcg ggcctgcagc ccgacagcga
     2161 ggtcggcttc catatccacg aggccggcga ctgcagctcg ggcgacggca tgagcgccaa
     2221 gggccacttc aatcccgagg gcaagccgca cggccatccc gcgagccgcg agcgccatgc
     2281 cggggacatg ccgatgctca gggcggacgc gagcggcaac gccagcattt ccgccgacct
     2341 cgacatcatc accgtcgct
//
LOCUS       DASYJA010000480         2690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_71499, whole genome shotgun sequence.
ACCESSION   DASYJA010000480 DASYJA010000000
VERSION     DASYJA010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71499"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VGO02_10460"
     CDS             <1..254
                     /locus_tag="VGO02_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478145.1"
                     /translation="IHSSRTVGGVFLAAAVRALAFALRLAGNIAKHACKVLIRLYDVV
                     IVLPLLAERLVRGARHRHAGKRGPKAANDVDIDVERTHA"
     gene            247..897
                     /locus_tag="VGO02_10465"
     CDS             247..897
                     /locus_tag="VGO02_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV7478146.1"
                     /translation="MLKKLLAGAAIFLATGCSDPRDHTHAVYMLVDTSGTYAAEAGKA
                     KLIINYLLGTLQPGDSLAVARVRSRSFSEKDIVAKATFDARPSQANAQKRSFQARIDD
                     AFRSVPGSQYTDITGGMIQAAQFLNEAGAGTKTIIIFSDMQEELDKVTVRNFPIDLKN
                     IRVIAVNVVKLKTDNVDPRLYLGRLEAWQKRVQGAGAVEWRVINDLEKLDRILSRG"
     gene            complement(894..1277)
                     /locus_tag="VGO02_10470"
     CDS             complement(894..1277)
                     /locus_tag="VGO02_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478147.1"
                     /translation="MHAFTKLVLYTERDGRAKWREEAVALGGGNPQAMLSELFPSSGY
                     QLRHSPLGFRSQWHCTPQAQWVFILGGEMEIGLQDKTSRVFRPGQHFFSADLLPSGAT
                     FDPAVHGHWSAQRGAHPLVTLFLKG"
     gene            1300..2412
                     /locus_tag="VGO02_10475"
     CDS             1300..2412
                     /locus_tag="VGO02_10475"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEV7478148.1"
                     /translation="MESADSAPAVALPPSLLERLLPLKPWLSAESSIGIEEGQSRTRL
                     VCALIGLAGLATIGQFRPLPDGVLFIAIAYPLFAIAYASHVRSHPAPTRTRRGSALLI
                     DNLMLSYIAYFGDAFAAYIAFYFLTTVGWGLRFGRHYLVMSTAIAVLGMCVNLLLSAY
                     WQENRMFWAVIVMAMLANTINVSILLRRIAWGNHRLEEKTEEISRLAWQDQLTKLPNR
                     RYFHERLSQTIASVDRTPRQIALILFDIDGFKSVNDTLGHEAGDRLLQEVSRVVGSRI
                     RQADTFARLGGDEFVVIMDSMRDQTDARLVAETILRTIAEIDLFAAQGLRIGASIGVA
                     CGAGRREIPDLLKQADRAMYEAKRAGKGCYRFAGES"
     gene            complement(2417..>2690)
                     /locus_tag="VGO02_10480"
     CDS             complement(2417..>2690)
                     /locus_tag="VGO02_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEV7478149.1"
                     /translation="GNWGCESSVTSKYVATIEVVDEGVIKVLTDRMGDLRVSPRYITL
                     APLSRSGQLMNEDDDKGSSVFRWRCGSTADGTDPELMNYLPSSCRG"
BASE COUNT          448 a          935 c          869 g          438 t
ORIGIN      
        1 tgatccactc ctcgcgcacg gtcggcggcg tgttcctcgc cgcggcagtg cgcgcgctgg
       61 ctttcgcgct gcgcctcgcc ggcaacatcg caaagcatgc gtgcaaggtg ctcatccgcc
      121 tctacgacgt agtgatcgtg ctgccgctgc tcgccgagcg cctggtgagg ggggcgcgcc
      181 accgccatgc gggcaagcgc gggccgaaag cagccaacga cgtcgatatc gacgtggagc
      241 gcacccatgc ttaagaaact gctcgcgggc gccgccatct tccttgccac aggctgcagc
      301 gacccgcgcg accatacgca tgccgtttac atgctggtgg atacctcggg cacctacgcc
      361 gccgaggccg gcaaggcgaa gctgatcatc aactacctgc tcggcacgct gcagccgggc
      421 gactcgctgg cggtggcgcg cgtgcgcagc cgcagcttca gcgagaagga catcgtcgcc
      481 aaggcgacct tcgacgcgcg cccgtcgcag gccaacgcgc agaagcgctc cttccaggcg
      541 cgcatcgacg acgccttccg cagcgtgccg ggaagccagt acaccgacat caccggcggc
      601 atgatccagg ccgcgcagtt cctgaacgag gcgggcgccg gcaccaagac catcatcatc
      661 ttctccgaca tgcaggagga gctcgacaag gtcacggtgc gcaacttccc tatcgacctg
      721 aagaacatcc gcgtcatcgc ggtcaacgtg gtcaagctga agaccgacaa cgtcgacccg
      781 cggctctacc tcggccgcct ggaggcctgg cagaagcgcg tccagggcgc cggcgccgtc
      841 gagtggcgcg taatcaacga cctcgagaag ctcgaccgga tcctttcccg gggctagccc
      901 ttcaggaaaa gcgtcaccag cggatgcgcg ccgcgctgcg cgctccagtg cccgtgcacg
      961 gcgggatcga aggtcgcgcc cgagggcagc aggtcggcgg agaagaaatg ctggcccggg
     1021 cggaagacgc ggctcgtctt gtcctgcagt ccgatttcca tctcgccgcc gaggatgaag
     1081 acccactgcg cctgcggcgt gcaatgccac tgactgcgga agcccagcgg gctgtggcgc
     1141 agctggtaac cggacgaagg gaataactcc gagagcatcg cctgcggatt gccgccgccc
     1201 agcgctaccg cttcctcgcg ccatttcgcc cggccgtcgc gctcggtgta cagcacgagc
     1261 ttcgtgaaag catgcatggt tgtgcgtatc ctttcggtta tggaatccgc cgattctgct
     1321 ccagccgtag cgctgccgcc cagcctgctc gagcggctgc tgccgctcaa gccctggctg
     1381 agcgccgaat cgtcgatcgg catcgaggag gggcagagcc gcacgcgcct ggtgtgcgcg
     1441 ctgatcggcc tcgccggcct cgccaccatc ggccagttcc ggccgctgcc cgacggcgtc
     1501 ctgttcatcg ccatcgcgta tccgctgttc gccattgcct acgcgagtca cgtgcggagc
     1561 caccccgcgc cgacgcgcac gcggcgcggg tcggcgctgc tgatcgacaa cctgatgctc
     1621 agctacatcg cctacttcgg cgatgcgttc gcggcgtaca tcgcgttcta cttcctcacc
     1681 accgtgggct gggggctgcg cttcggccgg cactacctgg tgatgagcac cgccatcgcc
     1741 gtgctcggca tgtgcgtcaa cctgctgctg agcgcctact ggcaggagaa ccgcatgttc
     1801 tgggcggtga tcgtgatggc gatgctggcc aacacgatca acgtgtcgat cctgctgcgg
     1861 cgcatcgcct ggggcaatca ccgcctcgag gagaagaccg aggagatctc gcgcctcgcc
     1921 tggcaggacc agctcaccaa gctgccgaac cggcgctact tccacgagcg cctgtcgcag
     1981 accatcgcct cggtggaccg cacgccgcgg cagatcgcgc tgatcctgtt cgacatcgac
     2041 ggcttcaagt cggtgaacga taccctgggc cacgaggccg gcgaccgcct gctgcaggag
     2101 gtgagccgcg tcgtcggcag ccgcatccgc caggccgaca ccttcgcccg gctgggcggc
     2161 gacgagttcg tggtgatcat ggactcgatg cgcgaccaga ccgacgcgcg cctggtcgcc
     2221 gagaccatcc tgcgaaccat tgccgagatc gatctgttcg cggcgcaagg gctgcgcatc
     2281 ggcgcgagca tcggcgtcgc ctgcggcgcc gggcggcgcg agattcccga tctgctcaag
     2341 caggccgacc gcgcgatgta cgaagcgaag cgcgccggca agggctgcta ccggttcgcg
     2401 ggggaaagct aggtcttcag cccctgcagc tgctgggcag gtaattcatg agctcggggt
     2461 cggtgccgtc cgccgtagaa ccgcagcgcc agcggaacac cgagctgccc ttgtcgtcgt
     2521 cctcgttcat gagctgccca ctgcggctga gcggggcgag ggtgatgtac ctgggggaaa
     2581 cgcgcagatc gcccatcctg tccgtgagta ccttgatcac gccctcgtcc accacctcga
     2641 tggtagccac gtacttcgaa gtcacgcttg attcgcagcc ccagttgcct
//
LOCUS       DASYJA010000481         2586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3068853, whole genome shotgun sequence.
ACCESSION   DASYJA010000481 DASYJA010000000
VERSION     DASYJA010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3068853"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1579)
                     /gene="lysS"
                     /locus_tag="VGO02_10485"
     CDS             complement(125..1579)
                     /gene="lysS"
                     /locus_tag="VGO02_10485"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEV7478150.1"
                     /translation="MEEEDNKLVEERRQKLNALRARGNAYPNDFRRKDSSDVLHREYG
                     EKSKEDLEKEKTPAVVAGRMVLKRVMGKASFATLQDGSGRIQAYITQEVPGYEEFKHW
                     DLGDLVGVEGHLFKTMKGELTVHAHRIFLLVKALRPLPEKFHGLSDHEMRYRQRYVDL
                     IVNPEVKDLFLKRTKIVQSIRDVLRNEGYMEVETPMMQPIPGGAVARPFKTHHNALDM
                     ELFLRIAPELYLKRLVVGGIERVFEINRNFRNEGISTRHNPEFTMLEWYCAYQDWTYM
                     MDLTERLIRAAAGVAEIVYQKNSIQLGKPFARLPIPEAIRRQGVEGDLRDRALLSKKL
                     SAMKVEHKSSQGWGALQLMLFEAIAEKHLIQPTFVTDFPAEVSPLARRRDADPDLTDR
                     FELFIDAKEIANGFSELNDPEDQAARFQEQMKAKEAGDQEAMYYDADYIRALEYGMPP
                     TAGAGLGIDRLVMLLTDSPSIRDVILFPQMRPES"
     gene            complement(1580..>2586)
                     /gene="prfB"
                     /locus_tag="VGO02_10490"
     CDS             complement(1580..>2586)
                     /gene="prfB"
                     /locus_tag="VGO02_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEV7478151.1"
                     /translation="ARLAALNRQLEDPKLWENPQRAQELGKEKKSLEAIVGTIGRVDG
                     GLSDAKELFDLARAEGDDATLSGVFSDIQKIGQDVAALEFRRMFSNPLDPNNCFMDIQ
                     AGAGGTEAQDWASMLLRMYLKYCDKKGFRAEILEQSDGEVAGIKNATLKITGDYAYGS
                     LRTENGVHRLVRKSPFDSNARRHTSFANVFVYPEVDDSIEVEINPADLRVDVYRASGA
                     GGQHVNKTESAVRITHIPSGIVVQSQNDRSQHRNRAECMAMLKSRPYELELRKLNAEA
                     DKLKAAQTDIEWGHQIRSYVLDQSRIKDLRTGVEKGATQNVLDGDLDDFITASLKQGV
                     "
BASE COUNT          398 a          792 c          835 g          561 t
ORIGIN      
        1 cctgcgcgtt ggcgagcgga accgcgcaga gcgccatcac cgcggcgaag gttcccatcc
       61 attggcgtgc catgcgactg ttctagcagc gccgcaagcg cggcgatatt ggaccttggt
      121 cccgctagga ctcgggccgc atttgcggaa agaggatcac gtcgcggatg ctggggctgt
      181 cggtgagcag catcaccaga cgatcgattc ccagacccgc tcccgcggtc ggcggcattc
      241 cgtactccag cgcgcggatg tagtcggcgt cgtagtacat cgcttcctgg tcgccggcct
      301 ccttggcctt catttgttcc tggaaccggg ctgcctggtc ctccgggtca ttcagctcgg
      361 agaagccgtt ggcgatctcc ttggcgtcga tgaacagctc gaagcggtcg gtgaggtcgg
      421 ggtcggcgtc gcgccgccgc gcgagcggcg acacctcggc cggaaagtcg gtgacgaagg
      481 tgggctggat caggtgcttc tctgcgatgg cttcgaacag catgagctgc agcgcgcccc
      541 agccctggct gctcttgtgc tcgactttca tggcagataa ttttttggac aacaaggcgc
      601 ggtcgcgcaa gtccccctca acgccttgtc tgcggatcgc ttcggggatc ggcaggcggg
      661 cgaagggctt gcccagctga attgaattct tttgataaac aatttccgca acgcccgccg
      721 ccgcgcggat cagccgctcg gtgaggtcca tcatgtaagt ccagtcctgg taggcgcagt
      781 accactcgag catggtgaac tcgggattat ggcgggtcga gatgccttcg ttcctgaagt
      841 tgcggttgat ctcgaacacg cgctctatgc cgcccaccac caggcgcttg aggtagagct
      901 cgggagcgat gcgcaggaac agctccatgt cgagcgcgtt gtggtgcgtc ttgaacggcc
      961 ttgccaccgc gccgccgggg atcggctgca tcatcggcgt ctcgacttcc atgtagcctt
     1021 cgttgcgcag gacgtcgcgg atcgactgga ctattttcgt tcttttcaag aaaagatctt
     1081 tgacttcggg gttgacgatc aggtcgacgt agcgctggcg gtagcgcatc tcatggtcgg
     1141 acaggccatg aaacttctcc ggcagaggtc tcaacgcttt cactaataaa aatattctat
     1201 gggcgtgaac cgtcagctcg cccttcatgg tcttgaagag gtggccctcg acgccgacca
     1261 ggtcgcccag gtcccagtgc ttgaactcct cgtagcccgg gacttcctgc gtaatgtacg
     1321 cctggatgcg gccgctgccg tcctgcagcg tggcgaagct cgccttgccc atgacgcgct
     1381 tgagcaccat gcggccggcg actactgccg gagtcttctc cttttccagg tcctctttgc
     1441 tcttttcccc gtattcacga tgcaggacat ctgaagaatc cttgcggcgg aaatcattgg
     1501 ggtaggcgtt gcccctggcg cgcagcgcgt tgagcttctg cctgcgctct tcgacgagct
     1561 tgttgtcttc ttcttccatc tacactccct gcttcaggct cgcggtgatg aagtcgtcga
     1621 ggtcgccgtc gagcacgttc tgcgtggcgc ctttctcgac gccggtgcgc aggtccttga
     1681 tgcgcgactg gtcgaggacg taggagcgga tctggtggcc ccactcgatg tcggtctgcg
     1741 cggccttgag cttgtccgcc tcggcattga gcttgcgcag ctcgagctcg taggggcgcg
     1801 atttgagcat cgccatgcac tcggcgcggt tgcggtgctg cgagcggtcg ttctggctct
     1861 gcaccacgat gccgctcggg atgtgggtga tgcgcaccgc cgactcggtc ttgttgacgt
     1921 gctggccgcc ggcgccggag gcgcggtaga cgtcgacgcg caggtcggcc gggttgatct
     1981 cgacctcgat cgagtcgtcg acctcgggat agacgaagac gttggcgaag gaggtgtggc
     2041 gccgtgcgtt cgagtcgaac ggactcttgc gcaccaggcg gtgcacgcca ttctcggtgc
     2101 gcaggctgcc gtaggcgtag tcgccggtga tcttgagcgt ggcgttcttg atgccggcca
     2161 cctcgccgtc ggactgctcg aggatttcgg cgcggaagcc cttcttgtcg cagtacttca
     2221 ggtacatgcg cagcagcatc gacgcccagt cctgcgcctc ggtgccgccg gcgccggcct
     2281 ggatgtccat gaagcagttg ttcgggtcga gcgggttgga gaacatgcgg cggaactcga
     2341 gcgccgcgac gtcctggccg attttttgaa tatcggaaaa aacaccggaa agcgtcgcgt
     2401 cgtcaccttc cgcgcgggcc agatcgaaca gctctttcgc atccgacagg ccgccgtcga
     2461 cgcggccgat cgtgccgacg atggcctcga gggacttctt ttccttgccc agctcctgcg
     2521 cgcgctgcgg gttctcccac agcttggggt cttcgagctg ccggttcagc gccgcgaggc
     2581 gcgcct
//
LOCUS       DASYJA010000482         3282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1614880, whole genome shotgun sequence.
ACCESSION   DASYJA010000482 DASYJA010000000
VERSION     DASYJA010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614880"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(301..897)
                     /locus_tag="VGO02_10495"
     CDS             complement(301..897)
                     /locus_tag="VGO02_10495"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV7478152.1"
                     /translation="MPLPEKFLRELSREEVNELPIKRYEGEVRLVESAQELERVAAAW
                     REERAVGFDTETRPAFKPGESYLPALAQIATARRVYLVPLQRLDGSEALRSLLEAPRT
                     VKAGVGMPDDMRALRKSFEFAEKAVVDLGVVARKHGLKQSGVRYLAALLLGTRIPKGT
                     KTSNWAASRLSPQQIAYAATDAWICRELYLKFEELKLL"
     gene            926..1270
                     /locus_tag="VGO02_10500"
     CDS             926..1270
                     /locus_tag="VGO02_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478153.1"
                     /translation="MAKKKARGPRHHHNVYVIELDPRILNHYRFRKANPNRDLLKPCV
                     YVGCTGLTPEERFAKHKAGIRANKYVQKYGLRLLPRLYAYANPMPYEAARDMEVELAI
                     ALQEEGYAVWQA"
     gene            complement(1227..2486)
                     /locus_tag="VGO02_10505"
     CDS             complement(1227..2486)
                     /locus_tag="VGO02_10505"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV7478154.1"
                     /translation="MSFSSLGLDPSLLENLTRLGYVEPTPVQREAIPAVLAGGDLLVS
                     SQTGSGKTAAFVLPGLQRLRTPSTRMSKGPRMLVLTPTRELAMQVQKAIHGYCRGQNL
                     MTALLVGGMPFGKQLDQVRKFVDVAIATPGRLKDHLDRGSMDLARVELLVLDEADRML
                     DMGFQEEIDSIIARVPKLRQTLLFSATLAGVVGQLAGRVTRSPKRIEVRGKEESKPDI
                     TQHALIADNQEHKGRMLDSLLRETEVEQALVFTATKRAAAELTSSLVMKGFTAGALHG
                     DMHQHERTRTLNQLREGRMRVLVATDVAARGIDVPGINLVVNFDAPRLAEDYLHRIGR
                     TGRAGRAGIAVTFLGHEDRNLVKNIERFTGNKVSMMEIVGLEPRTKFLESRDRGNRGQ
                     SPKPRRSENRALTPRPATRRSPLPAGR"
     gene            2778..2993
                     /locus_tag="VGO02_10510"
     CDS             2778..2993
                     /locus_tag="VGO02_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV7478155.1"
                     /translation="MNWDRIEGNWKQVKGKVKEQWGKITDDELDRIAGKRDQLVGKIQ
                     NSYGIGKDEAEKQIKDWEARQSGLDVE"
     gene            3013..3198
                     /locus_tag="VGO02_10515"
     CDS             3013..3198
                     /locus_tag="VGO02_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478156.1"
                     /translation="MEQNQRQSQQQQWQGPERRQSQGPHQGEERRKPQSGLSTQERKD
                     EQQDRAQQQAADQTDIH"
BASE COUNT          571 a         1110 c         1068 g          533 t
ORIGIN      
        1 ggagggcatc ccgacgcccg ttttcttcgt cgatccggtg caccacaccc agtagttttc
       61 attacagcgc caatcacgct ttgcacgatt gacgaagcag gacagaggag tagattggaa
      121 ccgagtctcg ccggggtcgc ccacacggca atgcagcggg attcccggcg actgtcggga
      181 taccggaccc ttcggtggac tcgcgcggca gactcagtcc gcgtatgcat tgaatgcgcc
      241 cagctaggcg cagcccgtca acgggcaccc aaggccccgc ccccgtgcgg ggcctttcat
      301 ttagagcagc ttcaattcct cgaacttcag atacagctcg cggcagatcc acgcgtcggt
      361 cgcggcgtag gcgatctgct gtggcgagag ccgcgacgcg gcccagttcg aggtcttggt
      421 gcccttcggg atgcgcgtgc cgaggagcag cgccgcgaga tagcgcacgc ccgactgctt
      481 gaggccgtgc ttgcgcgcca ccacgccaag atcgacgacc gccttctccg cgaactcgaa
      541 gctcttcctc agcgcgcgca tgtcgtcggg catgccgacg cccgccttga cggtgcgcgg
      601 cgcctcgagg aggctgcgca gcgcctcgga gccgtccagg cgctgcagcg gcacgaggta
      661 caccctgcgc gcggtggcga tctgcgcgag ggcgggcagg tagctctccc ccggcttgaa
      721 cgccgggcgc gtctccgtat cgaagccgac cgcgcgctcc tcgcgccacg ccgccgcgac
      781 gcgctcgagc tcctgcgcgc tctccaccag gcgcacctcg ccctcgtagc gcttgatcgg
      841 cagctcgttg acttcttcgc gcgagagctc gcgcaggaat ttttcaggca gcggcatggg
      901 aggggagtgt aggcaataat tgaatatggc gaagaagaag gcccggggac cccgccacca
      961 ccacaacgtc tacgtgatcg agctcgaccc gcgcatcctc aaccactacc gcttccgcaa
     1021 ggccaacccg aaccgcgacc tgctcaagcc ctgcgtctac gtcggctgca ccggcctcac
     1081 gcccgaggag cgcttcgcca agcacaaggc cggcatccgc gccaacaagt acgtgcagaa
     1141 gtacggcctg cgcctgctgc ccaggctcta cgcctacgcc aaccccatgc cctacgaggc
     1201 ggcgcgcgac atggaagtcg agctggctat cgccctgcag gaagagggct acgccgtgtg
     1261 gcaggcctag gggtcagagc ccgattttcg cttcttcttg gtttcgggct ctgaccccga
     1321 tttccgcgat cccggctttc cagaaacttc gtccgcggct ccagcccgac gatctccatc
     1381 atgctcacct tgttgccggt gaagcgctcg atgttcttca ccaggttgcg gtcttcgtgg
     1441 ccgaggaagg tcacggcgat gccggcgcgt cccgcgcggc cggtgcggcc gatgcggtgc
     1501 aggtagtcct ccgccaggcg cggcgcgtcg aagttcacca ccaggttgat ccccggcacg
     1561 tcgatgccgc gcgcggcgac gtcggtcgcc accagcacgc gcatgcggcc ctcgcgcagc
     1621 tggttcagcg tgcgcgtgcg ctcgtgctgg tgcatgtcgc cgtgcagcgc gccggcggtg
     1681 aagcctttca tcaccagcga gctggtcagc tcggcggccg cgcgcttggt ggcggtgaag
     1741 acgagcgcct gctcgacttc cgtctcgcgc aggagcgaat cgagcatgcg gcccttgtgc
     1801 tcctggttgt cggcgatgag cgcgtgctgc gtgatgtcgg gcttgctctc ttccttgccc
     1861 ctgacctcga tgcgtttcgg gctgcgcgtg acgcgcccag ccagctggcc gaccacgccg
     1921 gcgagcgtcg ccgagaagag cagcgtctgg cgcagcttcg gcacgcgcgc gatgatcgag
     1981 tcgatctcct cctggaagcc catgtcgagc atgcggtcgg cttcgtcgag caccagcagc
     2041 tcgacgcgcg cgaggtccat gctgccgcgg tccaggtggt ccttgaggcg gccgggcgtg
     2101 gcgatggcca cgtcgacgaa cttgcgcacc tggtcgagct gcttgccgaa gggcatgccg
     2161 cccaccagca gcgcggtcat caggttctgc ccgcggcagt agccgtggat cgccttctgc
     2221 acctgcatcg ccagctcgcg cgtcggcgtg agcaccagca tgcgcgggcc cttgctcatg
     2281 cgcgtcgacg gcgtgcgcag gcgctgcagc cccggcagca cgaaggccgc ggtcttgccg
     2341 ctgcccgtct gcgacgagac cagcaggtcg ccgccggcga gcaccgcggg aatcgcctcg
     2401 cgctgcaccg gcgtcggctc gacgtagccc aggcgcgtca ggttctccag cagggacggg
     2461 tcgagaccga gcgacgaaaa gctcatggca tttcctttgg tggcgccaac cttattaccg
     2521 cgatgccacc gaagggaagc ttcgatgaag gactgcgaga gggcgggcgg atcgaactgc
     2581 gaggcgcgca cgataacatg ggtcagagcc ccgaattttc tttttttagg gctctgaccc
     2641 cgatttttct cgccctgacc ccgaattttg ggcgtcgtca ttcgcacgac ctttcctatc
     2701 ggcatccagc ggcatcgccc agcgccacgc ctcggtataa cagttgcctc ggcagtacca
     2761 ccaatggagg agcacgcatg aactgggacc gcatcgaagg caactggaag caagtcaaag
     2821 gcaaagtgaa ggagcagtgg ggcaagatca ccgacgacga gctggaccgc atcgccggca
     2881 agcgcgacca gctggtcggc aagatccaga actcctacgg catcggcaag gacgaggccg
     2941 agaagcagat caaggactgg gaagcgcgcc agagcggcct cgacgtcgag taggcacgca
     3001 ggaggcaaca ccatggagca gaaccagcgg caatcgcagc agcagcaatg gcagggcccc
     3061 gagcggcgcc agtcgcaggg tccccaccag ggcgaggagc gcaggaagcc gcagtccggc
     3121 ctgagcacgc aggagcgcaa ggacgagcag caggatcgcg cccagcagca ggcggcggac
     3181 cagaccgaca tccactgaag taaggggtca gacccccgtt ttttcttttt gtcttggggt
     3241 ctgaccccag ctttacccta gaagcgaaac ggcagcgcca cg
//
LOCUS       DASYJA010000483         3350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_253618, whole genome shotgun sequence.
ACCESSION   DASYJA010000483 DASYJA010000000
VERSION     DASYJA010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253618"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VGO02_10520"
     CDS             <1..474
                     /locus_tag="VGO02_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEV7478157.1"
                     /translation="TSGSAEKLAKLKSLGLDVALRTRASFSKEALEATGGQGVNLIVN
                     TVGGSVFAEGIRTLAFQGRMAIVGYVDGVLKGEIDLDAVHAKRLRIFGVSNKLRNAEQ
                     RAATVRGFTADFLPLIAAGRIAPLVDKVFAFGELAAAKAYMEADKHVGKIVLRMP"
     gene            complement(461..1201)
                     /locus_tag="VGO02_10525"
     CDS             complement(461..1201)
                     /locus_tag="VGO02_10525"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEV7478158.1"
                     /translation="MKPEDATKLLHAYVDGELDPAASLELEAQLAADPSLRAAHEHLR
                     ALSAEIQSKADYRGAPEGLRARIESSLAADEPSPPARRHASRRLAALALTGIAAIALI
                     LAAVVFRPADNERLLQDILASHARATLGQRMMDVASSDRHTVKPWLNSRLPFSPPVAD
                     YASLGYELAGARVDYAGGRPVAVLLYKRRKHTIEVFVFPGADAPARAVSRDGLNLETF
                     AQGGMTYWLLSDVAREDLEGLARLLRAS"
     gene            complement(1198..1725)
                     /locus_tag="VGO02_10530"
     CDS             complement(1198..1725)
                     /locus_tag="VGO02_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV7478159.1"
                     /translation="MSDRRARFEQLVLPHLDAAFNLARWLTRNDHDAEDVVQEAMLRA
                     YRFCDRLRGEPRPWLLAIVRNACFTWLKANRPADLVGGADDDTRADEAAGPETLAART
                     FERKLLNEAIAALPAPFREVLVLRELEDLSYREIAQIAGVPMGTVMSRLARARRLLAE
                     SIRVITRASRPKVHE"
     gene            complement(1750..2106)
                     /locus_tag="VGO02_10535"
     CDS             complement(1750..2106)
                     /locus_tag="VGO02_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV7478160.1"
                     /translation="MKPRRRWLLAAAAVPLAAALARSARSEERVVRIASRKFAFDPDE
                     VTLKAGEPVVLELTTDDVHMGFKSVDLGLRADILPGRVTRLRLTPQKAGAFPFFCDVF
                     CGDDHELMNGVLRVVA"
     gene            complement(2103..2987)
                     /locus_tag="VGO02_10540"
     CDS             complement(2103..2987)
                     /locus_tag="VGO02_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV7478161.1"
                     /translation="MSIDRRQFLKLAGLGGVVLVSGCAGARDPYSAAADEFFFVQLSD
                     THWGFQGAPNPDAQGTLPKAIAAVNALPVQPDFVVFTGDLTHTTDDPKERRRRLAQFK
                     EIAGGLRVKSVRFMPGEHDGSLDRSAAYQEFFGPTHYTFDHKGVHFIALDNVSDPAGS
                     IGDEQLAWLKQDLDRQERAARIVVLTHRPLFPLFPQWDWATGDGERALELLMPYSNVT
                     VFYGHIHQEHHFKTAHIEHHAAKSLIFPLPAPGSQPRRAPLAWDPAQPYRGLGFRQVE
                     AEEKPVDYKIRELPVSGA"
     gene            3118..>3350
                     /locus_tag="VGO02_10545"
     CDS             3118..>3350
                     /locus_tag="VGO02_10545"
                     /inference="COORDINATES: protein motif:HMM:NF042483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HEV7478162.1"
                     /translation="METKMKKLLTSLTLALVLIAPQAQAGMIETPAASERERVKELVA
                     RPELAQRLKQMGIAPEEVQGRIDAMTDEEVRSLA"
BASE COUNT          532 a         1086 c         1205 g          527 t
ORIGIN      
        1 acctccggct ctgccgagaa gctggcaaag ctcaaaagcc tcggtttgga tgttgccctg
       61 cggaccagag cgagtttttc caaggaagcg cttgaagcga ccggagggca gggtgtcaac
      121 ttgattgtga atacggtagg aggctccgta tttgcagagg gcattcgtac gctggcgttc
      181 cagggccgta tggcgatcgt cggctacgtc gacggcgtgc tgaagggcga gatcgacctc
      241 gacgcggtgc acgcgaagcg actgcgcatc ttcggcgtct ccaacaagct gcgcaacgcg
      301 gagcagcgcg cggccactgt gcgcggcttc actgcggatt tccttcccct gattgccgcc
      361 ggccgcatcg cgccgctcgt cgacaaggtg tttgcgttcg gcgagctcgc cgcggcgaag
      421 gcctacatgg aagccgacaa gcacgtcggc aaaattgtgc tcaggatgcc ctgagcaggc
      481 gggcaagccc ctcgaggtct tcgcgcgcaa cgtcggacaa gagccagtag gtcatcccgc
      541 cctgtgcaaa ggtttccagg ttgagcccgt cgcgtgaaac ggcgcgcgcc ggggcgtctg
      601 cgcccgggaa aacgaacacc tcgatggtgt gcttgcggcg cttgtacagc aggaccgcga
      661 ccggccggcc gcccgcgtag tcgacgcgcg cgccggcgag ctcgtatccc agcgatgcgt
      721 aatccgccac cggcggcgag aacggcaggc gggagttgag ccacggcttc accgtgtgcc
      781 ggtcggagga ggcgacgtcc atcatgcgct ggccgagcgt cgcgcgtgcg tggcttgcaa
      841 gaatgtcctg cagcaggcgc tcgttgtcgg cgggccggaa caccacggcg gcaagaatca
      901 gggcgatcgc ggcgatgccc gtcagggcga gcgccgccag ccgtcggctc gcgtgccgcc
      961 gtgccggggg ggacggctcg tccgccgcga gcgaggactc gatccgcgcg cgcagccctt
     1021 ccggcgcgcc gcggtagtcg gccttgctct ggatctcggc ggagagcgcg cgcaggtgct
     1081 cgtgcgccgc ccgcagcgac ggatcggcgg ccagctgcgc ctcgagctcg aggctggccg
     1141 ccgggtcgag ctcgccgtcg acgtaggcgt gcagcagctt ggtggcgtct tccggcttca
     1201 ctcgtgcacc ttggggcgcg aagcgcgcgt gatcacgcgg atggactcgg ccagcagccg
     1261 gcgcgcgcgg gcgagccgcg acatcaccgt gcccatgggc acgccggcga tctgcgcgat
     1321 ctcgcggtag gaaaggtcct ccagctcgcg cagcaccagc acctcgcgga acggcgcggg
     1381 caacgcggca atcgcctcgt tgagcagctt gcgctcgaac gtgcgtgcgg cgagcgtctc
     1441 cgggccggcg gcttcgtcgg cccgcgtatc gtcgtcggcc ccgccgacca ggtcggcggg
     1501 gcggttcgct ttcagccagg taaagcaggc gttgcgcacg atggccagca gccacggccg
     1561 gggctcgccg cgcaagcggt cgcagaagcg gtaggcgcgc agcatcgctt cctgcaccac
     1621 gtcctcggcg tcgtggtcgt tgcgcgtgag ccagcgcgcg aggttgaacg ccgcgtcgag
     1681 gtgcggtagc accagctgct cgaatctggc gcggcgatcg ctcaaggccc gctcatggta
     1741 ccgcggggct caggcgacga cgcgcagcac gccgttcatg agctcatggt cgtcgccgca
     1801 gaacacgtcg cagaagaacg ggaacgcgcc ggccttctgc ggcgtcaggc gcaggcgcgt
     1861 cacccggccc ggcaggatgt cggcgcgcag gccgaggtcg accgacttga agcccatgtg
     1921 gacgtcgtcg gtcgtcagct cgagaaccac gggctcgccc gctttcagcg tgacttcgtc
     1981 gggatcgaaa gcgaacttgc gcgaggcgat gcgtaccacg cgctcctccg agcgcgcgct
     2041 gcgggcgagg gcggcggcca gcggcacggc cgcggcggcg agcagccagc gccggcgcgg
     2101 cttcatgccc cgctcaccgg cagctcgcgg atcttatagt cgacgggttt ctcttcggcc
     2161 tcgacctggc ggaagcccag accccggtac ggctgcgccg ggtcccacgc aagcggcgct
     2221 cgcctgggtt gcgagcccgg cgcaggcagc gggaagatca gcgacttggc ggcatggtgc
     2281 tcgatgtgcg cggtcttgaa atggtgttcc tggtggatgt ggccgtagaa caccgtgacg
     2341 ttgctgtagg gcatgagcag ctccagcgcc cgttcgccgt cgccggtggc ccagtcccat
     2401 tgagggaaca gcggaaacag gggacggtgg gtgagcacca cgatgcgcgc cgcgcgctcc
     2461 tgccgatcga gatcctgctt cagccaggcg agctgctcgt cgccgatgct gccggccgga
     2521 tcggacacgt tgtcgagcgc gatgaagtgg actcccttgt gatcgaacgt gtaatgcgtc
     2581 ggcccgaaga attcctggta ggccgcgctt cgatcgagcg agccgtcgtg ctcgccgggc
     2641 atgaacctca cgctcttcac ccgcaggccg ccggcgattt ccttgaactg cgcgaggcgc
     2701 cggcggcgct ccttcggatc gtcggtggta tgcgtcaggt cgccggtgaa tacgacgaaa
     2761 tcgggctgca cgggcagcgc gttgaccgcg gcgatcgcct tgggcagggt gccctgggca
     2821 tcgggattgg gcgcgccctg gaacccccag tgcgtgtccg agagctgcac gaagaagaac
     2881 tcgtcggcgg ctgcggaata ggggtcgcgc gcgccggcgc atcctgaaac gagcaccact
     2941 ccgccaaggc ctgccagctt gaggaactgc ctgcggtcga tcgacatgtc ggtctccttt
     3001 cactgcaaga ccgaccggtg cgcgttttta ttcccggctg tagcccgatc gcgacccgcg
     3061 tggcgcggcc ggcgacatga aaccgccggg gcttagaata tagtcagggg gttttctgtg
     3121 gagacgaaga tgaagaaact gttgaccagt ctgaccctgg cgctggtact gatcgcccct
     3181 caggcgcagg ccggcatgat cgagacgccc gccgcgtccg aaagagagcg cgtcaaggag
     3241 ctggtagccc ggcccgagct cgcgcagcgg ctgaagcaga tgggaatcgc gcccgaagag
     3301 gttcaggggc ggatcgatgc catgaccgat gaggaggtgc gcagcctcgc
//
LOCUS       DASYJA010000484         6718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2796509, whole genome shotgun sequence.
ACCESSION   DASYJA010000484 DASYJA010000000
VERSION     DASYJA010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6718
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2796509"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..422
                     /locus_tag="VGO02_10550"
     CDS             183..422
                     /locus_tag="VGO02_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478163.1"
                     /translation="MYWLYGAGAHQLVRGAAAARRGLGQHRHRHRQRRVQLQLLVENQ
                     ICADGAATVVFIDMEKQKPVRIPEALRNLISEGIQ"
     gene            419..1105
                     /locus_tag="VGO02_10555"
                     /pseudo
     CDS             419..1105
                     /locus_tag="VGO02_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891596.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
     gene            1148..1765
                     /locus_tag="VGO02_10560"
     CDS             1148..1765
                     /locus_tag="VGO02_10560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEV7478164.1"
                     /translation="MAKRAAQELRDGYYVNLGIGIPTLVANYIPAGVNVTLQSENGLL
                     GIGPFPAENRVDADLINAGKQTVTTIPGSSFFSSSDSFAMIRGGHVDLSILGGLEVAE
                     NGDLANWMVPGKMVKGPGGAMDLVSGVRRVVVLMEHTSKDGASKVLKRCTLPITGKGV
                     ANMIITDLCVFEVEPGKGLVLKELHPGVTLEDVRAKTAAEFRVAL"
     gene            1807..3468
                     /locus_tag="VGO02_10565"
     CDS             1807..3468
                     /locus_tag="VGO02_10565"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV7478165.1"
                     /translation="MSKYVFVTGGVVSSLGKGIAAASLAAILESRGIRVTNIKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFQSSKMRRWNNFTTGQIYESVIKKE
                     RKGDYLGGTVQVIPHITDEITSFIKRGAGDAEVAIVEIGGTVGDIESLPFLEAIRQMG
                     LELGRNNVCYIHLTLVPYIASAGEIKTKPTQHSVKELREIGIQADALLCRTDRPVPEE
                     ERRKIALFTNVQKEAVISAWDVDSIYKIPTMLHEQMLDEIVCHKLQILAKAADLSAWR
                     KLVHAQANPEHELDIAFVGKYVDLQDSYKSLNEALTHAGIHTHTKVRIHYLDSEEIEK
                     NGVGPLEKMDAILVPGGFGKRGTEGKIRAIRYARENGIPYLGICLGMQLATIEFARNV
                     CGLAGANSTEFDRETPHPVVALITEWLDRTGQIEKRDDASDLGGTMRLGAQKCPVEPD
                     TLAFSIYGKEVNERHRHRYEVNNIYVPQLEAKGYKVSARTPSENLPEIMELPGHPFFV
                     GVQFHPEFTSTPRAGHPLFTAFVAAALRYRANVKRPGGTLSVVAA"
     gene            3465..4301
                     /gene="kdsA"
                     /locus_tag="VGO02_10570"
     CDS             3465..4301
                     /gene="kdsA"
                     /locus_tag="VGO02_10570"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEV7478166.1"
                     /translation="MKLCGFEAGLDKPLFLIAGPDTLESEQLCLDVAGKLKEHTAKLG
                     MPYIFKGSFDKANRTSGKSYRGPGMEEGLKILSQIKKKIGVPVLTDVHEDTPLKEVAA
                     VVDVIQTPAFLCRQTNFIRSAASQGKPVNIKKGQFLSPWEMKQVVEKATSTGNKQILV
                     CERGFSFGYNNLVVDMRGLAIMRDTGCPVVFDATHSVQLPGGQGTSSGGAREHISVLA
                     RAAVAAGVSGVFMETHPDPDNALCDGPNSWPLQLMPELLETLVELDRITKKKAFIEDK
                     VR"
     gene            4304..5587
                     /gene="eno"
                     /locus_tag="VGO02_10575"
     CDS             4304..5587
                     /gene="eno"
                     /locus_tag="VGO02_10575"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV7478167.1"
                     /translation="MSSIVDVVAREILDSRGNPTVEADVLLESGVMGRAAVPSGASTG
                     TREAIELRDGDKRRYGGKGVLKAVEAVNTEISEAIVGLDATEQTFIDKTLIDLDGTEN
                     KGRLGANAILAVSMAAAKSAAEESGLPLYRYFGGSGAMKMPVPMMNVINGGAHANNNL
                     DMQEIMIVPVGAQSFREAMRCGAEVFQKLKSLIDSKGLPTTVGDEGGFAPDMPTHAAA
                     IELLLKAIEGAGYTPGQEVVLALDCAASEFYREGRYVLESEKKNLDASQFADYLADLA
                     GRYPIVSIEDGMAEGDWEGWKILTQKLGRKLQLVGDDLFVTNVKLLREGIKQGVANAI
                     LVKVNQIGTLTETFAAIELAKRAAYGTVISHRSGETEDTTIADIAVGINALQIKTGSL
                     SRSERVAKYNQLLRIEEDLGDSVSYIGREAFAQLR"
     gene            5584..5904
                     /gene="ftsB"
                     /locus_tag="VGO02_10580"
     CDS             5584..5904
                     /gene="ftsB"
                     /locus_tag="VGO02_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEV7478168.1"
                     /translation="MKILAAVLGALILLVQYPLWLGKGGWMRAWQIEQDFNTQKNSNL
                     RLEVRNAGLSAEVTDLKTGTEAIEERARYELGLVRGDEIFFQIEDKIPGQSPISGSKP
                     SGAK"
     gene            5925..6380
                     /locus_tag="VGO02_10585"
     CDS             5925..6380
                     /locus_tag="VGO02_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011881036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEV7478169.1"
                     /translation="MALASAALCGEPLRNWFNDPYFQVRSGIAGCPLPLGPFTTESDM
                     KKQTHYRAERGTTCWLAKKCDKPNSYLYDPEIAAAAKARFEASRALRDASLWVTVQRR
                     WVQVDGCVPSAYAPGALEKLLADIPDVEHVIVNVRKPGAKLPYRARADP"
     gene            6377..>6718
                     /locus_tag="VGO02_10590"
     CDS             6377..>6718
                     /locus_tag="VGO02_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478170.1"
                     /translation="MRTRTVMAALCLGLAGCASTTVNKTGPGAQERWCDTSLRAIVQW
                     DTEWRPDQKDVAAREAAAGRAIERFFAGSRCFARTVVRREGRVDPNYPRVVFITVREL
                     GPVVRIGPALFE"
BASE COUNT         1312 a         2187 c         2193 g         1026 t
ORIGIN      
        1 cttgatgcga atcgcggcgg cgaggccggc ggggccggcg ccgacgatca ctacgtcgta
       61 cagcatcgat tcgcgcgcca ttcgggaatt ataatcgcgg tgaaatgaac gatagaaagc
      121 tggtccatgt tgatcagttc cgatccgctg ggtgaccgcg ttgtgtcacg tcgcataaca
      181 cggtgtactg gctatatgga gcaggcgcgc atcagctggt tcgaggcgct gctgccgcgc
      241 ggcgaggcct ggggcagcac cggcatcgtc atcgccaacg ccgcgtgcaa cttcaattgc
      301 tcgtggaaaa ccagatctgt gccgacggcg ccgctacggt ggtgttcatc gacatggaga
      361 agcagaagcc ggtgcgcatt ccggaggccc tccggaacct tattagcgaa ggaatccaat
      421 gaaaaaggtc tatccagacg ccgctgccgc cctgcaggga ctgctgcgcg acgacatgtt
      481 cgtggccgcg ggcggtttcg ggctgtgcgg catccccgag aacttcatcc aggcgctggt
      541 cgacagcaag atcaagggcc tgaccatcgt cggcaacaac gccggcgtgg acgacttcgg
      601 catgggcctg ctgctcaaga cccggcaggt gaagaaggtg atcgcctcgt acgtcggcga
      661 gaacaaggag ttcgagcgcc aggtgctcgc cggcgagctc gacctgcagc ttacgccgca
      721 gggaacgctc gccgagaagc tgcgcgccgg cggcgccggc atcccgggct tctatacgcg
      781 caccgcagcc ggtaccaagc tcgccgaagg caaggagacc aagactttcg acggcaagga
      841 atacgtgctg gaggagggca tccgcaccga cctgtccatg gtcaaggcct ggaagggcga
      901 caaagccggc aacctcgtct accgcatgac ctcgcgcaac ttcaatccga tgatcgccac
      961 ctgcggcaag gtgtgcgtcg cggaggtgga gcaactggtc gaaatcggcg agctcgaccc
     1021 cgaccagatc cacacgcccg gcatctatgt ggaccgcatc atccaggggg cccgctacga
     1081 gaagcggatc gagttccgca cggtcaccgg cgccgtcggc aagaaggatt caccgatccg
     1141 cgagctgatg gcgaagcgcg ccgcgcagga gttgcgcgac ggctactacg tcaacctggg
     1201 catcggcatc ccgacgctgg tggccaacta catcccggcc ggcgtcaacg tcacgctgca
     1261 gtcggagaac ggcttgctcg gcatcgggcc gttcccggcc gagaaccggg tcgacgcgga
     1321 cctgatcaac gccggcaagc agacggtcac cacgatcccc ggctcgagct tcttctccag
     1381 ctcggactct ttcgccatga tccgcggcgg ccacgtcgat ctgtccatcc tcggcggcct
     1441 cgaggtggcg gagaacggcg atctcgccaa ctggatggtg cccgggaaga tggtcaaggg
     1501 ccccggcggc gccatggacc tggtctcggg cgtgcgccgg gtcgtggtgc tgatggagca
     1561 cacctcgaag gacggcgcct ccaaagtgct gaagcgctgc acgctgccga tcaccggcaa
     1621 gggcgtggcc aacatgatca tcaccgacct gtgcgtgttc gaagtggagc cgggaaaggg
     1681 gctggtgctg aaggaattgc acccgggcgt gacactcgag gacgtgcgag cgaaaaccgc
     1741 ggccgagttc cgggttgcac tttgagtttt cgtgtaaact gctccattcc tacacgccgt
     1801 gggtaaatgt ccaagtatgt ctttgtaacc ggtggtgtag tttcctctct ggggaaaggc
     1861 atcgccgccg cctccctcgc cgcgatcctc gaatcgcgcg gcatccgcgt caccaacatc
     1921 aagctggacc cgtacatcaa cgtcgacccc gggacgatgt cgccgttcca gcacggcgag
     1981 gtattcgtca ccgaggacgg ggcggaaacc gacctcgacc tgggccacta cgagcgcttc
     2041 cagagctcga agatgcgccg ctggaacaac ttcaccaccg gccagatcta cgagtcggtg
     2101 atcaagaagg agaggaaggg cgactacctc ggcggcaccg tccaggtcat cccgcacatc
     2161 accgacgaga tcacgtcctt catcaagcgc ggcgccggcg acgccgaggt ggcgatcgtc
     2221 gagatcggcg gcaccgtggg cgacatcgag tcgctgccgt tcctagaggc gatccggcag
     2281 atgggcctgg agctgggccg caacaacgtc tgctacatcc acctgacgct ggtgccctac
     2341 atcgcgtcgg cgggcgagat caagaccaag ccgacgcagc actcggtgaa ggagctgcgc
     2401 gagatcggca tccaggccga cgcgctgctg tgccgcaccg accggccggt gcccgaggag
     2461 gagcggcgca agatcgccct cttcaccaac gtgcagaagg aggcggtgat ctccgcctgg
     2521 gacgtggact cgatctacaa gatcccgacc atgctgcacg agcagatgct cgacgagatc
     2581 gtctgccaca agctgcagat cctcgccaag gccgccgacc tgtcggcgtg gcgcaagctg
     2641 gtgcacgcgc aggccaaccc cgagcacgag ctcgacatcg ccttcgtcgg caagtacgtc
     2701 gacctgcagg actcctacaa gtcgctcaac gaggcgctca cccacgccgg gatccatacg
     2761 cacaccaagg tgcgcatcca ctacctcgac tcggaggaga tcgagaagaa cggggtaggg
     2821 ccgctcgaaa agatggacgc gatcctggtg cccggcggtt tcggcaagcg cggcaccgaa
     2881 ggaaagatcc gcgccatccg ctacgcgcgc gagaacggca tcccgtacct cggcatctgc
     2941 ctcggcatgc agctcgccac catcgagttc gcgcgcaacg tctgcggcct ggcgggcgcc
     3001 aacagcacag agttcgaccg cgagacgccg cacccggtgg tcgcgctgat caccgaatgg
     3061 ctcgaccgca ccggccagat cgagaagcgc gacgacgcct ccgatctggg cggcacgatg
     3121 cgcctcggcg cgcagaaatg ccccgtcgag cccgataccc tggcgttttc catctacgga
     3181 aaagaagtaa acgagcggca ccgccaccgc tacgaggtga acaacatcta cgtgccgcag
     3241 ctcgaggcca agggctacaa ggtctcggcg cgcacgccga gcgagaacct gccggagata
     3301 atggagctgc ccggccatcc gttcttcgtc ggcgtgcagt tccaccccga gttcacctcg
     3361 acgccgcgcg cgggccatcc gctgttcacg gcgttcgtcg cggctgcgct gagataccgc
     3421 gcgaacgtca aacgcccggg cggcacactt tcggttgtag cggcgtgaaa ctctgcgggt
     3481 tcgaagccgg gctggataag cccctgttcc ttattgcggg tccggacacg ctggaaagcg
     3541 agcagctctg cctcgacgtc gccggcaagc tcaaggagca cacggcgaag ctgggcatgc
     3601 cgtacatctt caaaggctcc ttcgacaagg cgaaccgcac ctcgggcaag agctatcgag
     3661 gcccgggcat ggaggagggt ttgaaaatac tctcccaaat aaaaaagaaa atcggcgtgc
     3721 ctgtgctcac cgacgtccac gaggacaccc cgctgaagga agtcgccgcg gtggtcgacg
     3781 tcatccagac gccggccttc ctctgccggc agaccaactt catccgcagc gccgcgtccc
     3841 agggcaagcc cgtcaacatc aagaagggcc agttcctgtc gccgtgggaa atgaagcagg
     3901 tggtcgagaa ggccaccagt accggtaaca aacagatact agtgtgtgag cgcggcttca
     3961 gcttcggcta caacaacctg gtagtcgaca tgcgcggcct ggccatcatg cgagacacgg
     4021 gctgcccggt ggtattcgac gcgacgcact cggtgcagct gccgggcggg cagggcacgt
     4081 cttcgggcgg cgcgcgcgag catatctccg ttctggcgcg cgccgcggtc gccgcgggag
     4141 tcagtggcgt gttcatggag acgcaccccg atccggacaa cgcgctgtgc gacgggccca
     4201 actcctggcc gctgcagctg atgcccgagc tgctcgagac gctggtggag ctggatcgga
     4261 ttaccaagaa gaaagctttt attgaggaca aggtgagata ggcatgagct ccatagtgga
     4321 tgtggtggca agggagatcc tcgactcgcg cggcaacccg acggtcgagg cggacgtgct
     4381 gctcgagtcc ggcgtgatgg gccgcgccgc ggtgccatcg ggcgcctcga ccggcacgcg
     4441 cgaggcgatc gagctgcgcg acggcgacaa gcgccgctac ggcggcaagg gcgtgctcaa
     4501 ggcggtcgag gcggtgaaca ccgagatctc cgaggccatc gtcggcctgg acgccaccga
     4561 gcagaccttc atcgacaaga cgctgatcga cctcgacggc accgagaaca agggccgcct
     4621 gggcgccaac gcgatcctcg ccgtgtcgat ggccgccgcc aagtccgcgg ccgaggaatc
     4681 cggcctgccc ctgtatcgct atttcggcgg ctcgggcgcg atgaaaatgc cggtgccgat
     4741 gatgaatgtg atcaacggcg gtgcgcacgc caacaacaac ctcgacatgc aggagatcat
     4801 gatcgtcccg gtcggcgcgc aatcgttccg cgaggcgatg cgctgcggcg ccgaggtttt
     4861 ccagaaactg aagtcgttga tcgattccaa ggggctgccg accacggtgg gcgacgaggg
     4921 cggcttcgcg cccgacatgc cgacgcacgc cgccgccatc gagctgctgc tgaaggcgat
     4981 cgagggcgcc ggctacacgc ccggccagga ggtggtcctc gcgctcgact gcgccgcatc
     5041 cgagttctac cgcgagggcc gctatgtcct ggaatcggag aaaaagaatc tcgacgccag
     5101 ccagttcgcc gactacctgg ccgacctcgc cggccgctac ccgatcgtgt cgatcgagga
     5161 cggcatggcc gagggcgact gggaaggctg gaagatcctc acgcagaagc tcggccgcaa
     5221 gctgcagctg gtcggcgacg atctgttcgt caccaacgtc aagctgctgc gcgagggcat
     5281 caagcagggc gtggccaacg ccatcctggt gaaggtcaac cagatcggca cgctcaccga
     5341 gaccttcgcc gcgatcgagc tcgccaagcg cgccgcctac ggcaccgtga tctcgcaccg
     5401 ctcgggcgag accgaggaca ccaccatcgc cgacatcgcg gtgggcatca acgcgctgca
     5461 gatcaagacc gggtcgctgt cgcgctccga gcgcgtcgcc aagtacaacc agctgctgcg
     5521 catcgaggaa gacctcggcg actcggtgag ctacatcggc cgggaggcct tcgcgcagct
     5581 aagatgaaga tcctcgccgc cgtcctgggt gcgctgatcc tgctggtcca gtacccgcta
     5641 tggctgggca agggcggctg gatgcgagct tggcaaatag aacaagattt caatacacaa
     5701 aaaaatagca acctaaggct ggaggtgcgc aacgccggcc tgtccgccga ggtgaccgac
     5761 ctgaaaaccg gcaccgaggc gatcgaggag cgcgcgcgct acgagctggg cctggtgcgc
     5821 ggcgacgaga tcttcttcca gatcgaagac aaaattccgg gtcagagccc gatttccggg
     5881 agcaagccgt ccggcgcgaa gtagggctcg cggcgctggc cggattggcg ctggcgtcgg
     5941 ccgcgctttg cggggagccc ctgcgcaact ggttcaacga cccgtatttc caggtgaggt
     6001 ccggcatcgc cggctgcccg ctgccgctcg gaccgttcac cacggaatcc gacatgaaga
     6061 agcagacgca ctaccgcgcc gagcgcggca ccacttgctg gctcgccaag aagtgcgaca
     6121 agccgaactc ctacctctac gacccggaga tcgcagccgc cgcgaaagcg cgcttcgaag
     6181 cctcgcgcgc cctgcgcgac gccagcctgt gggtcacggt gcagcgccgc tgggtgcagg
     6241 tcgacggctg cgtcccatcc gcctacgcac ccggcgcgct ggagaagctg ctagccgata
     6301 tccccgacgt cgagcacgtg atcgtcaacg tgcggaaacc tggcgccaag ctgccgtacc
     6361 gcgcgcgggc cgacccgtga ggacccgcac agtcatggcc gcgctatgcc tcggcctcgc
     6421 cggctgcgcc agcaccacgg taaacaagac cggcccgggc gcgcaggagc gctggtgcga
     6481 cacatccctg cgcgcgatcg tgcaatggga caccgagtgg cgtcccgacc agaaggacgt
     6541 agcggcgcgg gaagccgcgg ccggacgcgc gatcgagcgc ttctttgccg gctcgcgctg
     6601 ctttgcgcgc accgtagtac gtcgcgaggg cagggtcgat ccgaattatc cgagggtggt
     6661 tttcatcacg gtccgcgagc tcggaccggt ggtccgcatc gggcccgcgc tcttcgaa
//
LOCUS       DASYJA010000485         1680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1889899, whole genome shotgun sequence.
ACCESSION   DASYJA010000485 DASYJA010000000
VERSION     DASYJA010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889899"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VGO02_10595"
     CDS             <1..248
                     /locus_tag="VGO02_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7478171.1"
                     /translation="LLDEPMAGRGPEESKRMIALIERIRASVTVLLVEHDMDAVFQLA
                     DRISVLVDGRIIATDVPGKIRANPEVRRAYLGDEVAA"
     gene            241..942
                     /locus_tag="VGO02_10600"
     CDS             241..942
                     /locus_tag="VGO02_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV7478172.1"
                     /translation="MLEIKDLETAYGASQVLFKLDLQIEEGEAVTLLGRNGMGKTTTV
                     RSIMGLTRARGGAITFRGRRIEREAPDRIARLGIALVPEGRQIFPNLSVAENLIAFAG
                     NRSARQEPWTVERVFDLFPALKNRKDNMGNQLSGGEQQMLAIGRALVTNPHLLILDEA
                     TEGLAPLIREEIWQCLARLRAAGQTILVIDKYIERLVRLADRHTIIERGRVAWQGSSA
                     ALNADRGLWHRYLGV"
     gene            944..>1680
                     /locus_tag="VGO02_10605"
     CDS             944..>1680
                     /locus_tag="VGO02_10605"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7478173.1"
                     /translation="MDLRWPKSAAELETIQRERKRVAVERARQSSFLKKRIPTGKAED
                     IWHKIPPLTKEELRQIPPERFHDEFCIQPRSAAVEYWRSGGATGRPLFYPRSAEDSMH
                     GIVAFERAWALIGATAQDCAHISFPLGVHPVAHLYARAAINRGIGTVWCGSGTNTPSE
                     TQLELIDNLKPTVWLGMASYGLHLANLAEAKGFDLRNSTVKKIIVAAEPLSPVKRKKL
                     EDAWGAEVFDHFGMTEGAFVAGEGIGH"
BASE COUNT          312 a          568 c          544 g          256 t
ORIGIN      
        1 tgctgctcga cgagcccatg gcgggcaggg ggcccgagga gtcgaagcgc atgatcgcgc
       61 tcatcgagcg catccgcgcg agcgtcacgg tgctgctggt cgagcacgac atggatgcgg
      121 tgttccagct cgccgaccgc atctcggtgc tggttgacgg ccgcatcatc gcgaccgatg
      181 ttcccgggaa aatacgcgcc aacccggaag tgcgccgcgc ctacctggga gacgaagttg
      241 ctgcttgaga tcaaggatct ggagaccgcg tatggcgcaa gccaggtcct tttcaagctc
      301 gatcttcaga tagaagaagg tgaagcggtc accctgctcg gccgcaacgg catgggcaag
      361 acgaccaccg ttcgctccat catgggcctg acgcgtgcgc gcggcggcgc catcactttc
      421 cgtggcaggc gcatcgagcg cgaggcgccc gaccgcatcg cgcgcctggg catcgcgctg
      481 gtgcccgagg ggcggcagat ctttccgaat ctatcggtag ccgaaaacct tattgccttt
      541 gcaggcaacc gctccgcacg ccaggagccg tggactgtag agagggtatt cgatctgttt
      601 cctgcattga aaaaccgaaa agacaacatg ggcaaccagc tttccggcgg cgagcagcag
      661 atgctcgcca tcggccgcgc gctggtgacc aacccgcacc tgctgatcct cgacgaggcc
      721 accgagggcc tcgcgccgct gatccgcgag gagatctggc aatgcctggc ccggctgcgc
      781 gccgcgggcc agacgatcct ggtcatcgac aagtacatcg agcggctggt ccggctggcc
      841 gaccggcaca ccatcatcga gcgcggccgc gtcgcctggc agggcagctc ggccgcgctg
      901 aacgccgacc gcgggctgtg gcaccgctac ctgggggtct agagtggacc tgcgctggcc
      961 gaagtcggca gccgagctcg agacgatcca gcgcgaacgc aagcgagtcg ccgtcgagcg
     1021 tgcaagacag tcttcgttct tgaaaaaacg catccccacg ggaaaagcgg aagacatctg
     1081 gcacaagatc ccgccgctca ccaaggaaga gctgcggcag atcccgccgg agcgcttcca
     1141 cgacgagttc tgcatccagc cgcgcagcgc ggcggtcgag tactggcgct ccggcggcgc
     1201 caccggccgg ccgctcttct atccgcgctc ggccgaggat tcgatgcacg gcatcgtcgc
     1261 cttcgagcgc gcctgggcgc tgatcggcgc caccgcccag gactgcgcgc acatctcctt
     1321 cccgctcggc gtgcacccgg tcgcgcacct ctacgcgcgc gccgccatca accgcggcat
     1381 cggcaccgtg tggtgcggct ccggcaccaa cacgccttcc gaaacgcagc tcgagctcat
     1441 cgacaacctc aagccgaccg tctggctggg catggcgagc tacggcctgc atctggcaaa
     1501 tctcgcggaa gcgaagggct tcgacctgag gaacagcacg gtgaagaaaa tcatcgtcgc
     1561 cgccgagccc ctctcccccg tgaagcgaaa gaagctcgaa gatgcctggg gggcggaggt
     1621 gttcgaccat ttcggcatga ccgagggcgc cttcgtcgcc ggcgagggaa tcggccacca
//
LOCUS       DASYJA010000486         3025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2160773, whole genome shotgun sequence.
ACCESSION   DASYJA010000486 DASYJA010000000
VERSION     DASYJA010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2160773"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VGO02_10610"
     CDS             complement(<1..258)
                     /locus_tag="VGO02_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HEV7478174.1"
                     /translation="MIPDSFKQDLLNRVDIVDVVQRYVQLKKGGANFMGLCPFHGEKT
                     PSFTVSPSKQFYHCFGCGVHGNAIGFLMAYAGMGYVDAIKDL"
     gene            complement(269..721)
                     /locus_tag="VGO02_10615"
     CDS             complement(269..721)
                     /locus_tag="VGO02_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEV7478175.1"
                     /translation="MSTSLKDKITEDMKTAMRARDSARLSAIRLILSGIKQREVDERI
                     QLTDADVLSVIDKMIKQRRESIAQFEKASRNDLAEAEKFEIGVLSAYLPQQMGDAELA
                     QAVAAALAESGASGIKDMGKVMALLKPRLAGRADMGAVSSLVKAKLSG"
     gene            complement(729..941)
                     /gene="rpsU"
                     /locus_tag="VGO02_10620"
     CDS             complement(729..941)
                     /gene="rpsU"
                     /locus_tag="VGO02_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEV7478176.1"
                     /translation="MPTVRVKENEPFEVAMRRFKRTVEKTGLLTELRAREYYEKPTAE
                     RKRKLAAAVKRHHKRLRSQMLPQKMY"
     gene            995..1987
                     /gene="tsaD"
                     /locus_tag="VGO02_10625"
     CDS             995..1987
                     /gene="tsaD"
                     /locus_tag="VGO02_10625"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006027813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEV7478177.1"
                     /translation="MLILGIETSCDETGVALYDGGARRLLAHAVHSQVAMHEAYGGVV
                     PELASRDHIRRLAPLTRGVLDSSNRHFSELSGVGYTEGPGLAGALLVGASFAHGLAAA
                     LHIPSVGIHHLEGHLLSPLLSAEPPAFPFVALLVSGGHTQLMRVDGVGRYALLGETQD
                     DAAGEAFDKTAKLLGLGYPGGPALSKLAESGAPGRHRLPRPMLDSADLDFSFSGLKTA
                     VLTTLKKHHPDPADLARAFVDAVVEVLVAKCVRALDETGMARLVVAGGVGANRQLRAA
                     LNAEAARRGFRVFYPDAELCTDNGAMIAFAAALHFGEAALTGGFTVKPRWDLAA"
     gene            complement(1953..2576)
                     /gene="plsY"
                     /locus_tag="VGO02_10630"
     CDS             complement(1953..2576)
                     /gene="plsY"
                     /locus_tag="VGO02_10630"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEV7478178.1"
                     /translation="MQAVLTVLAAYLLGSVSFAIVVSRLMRLPDPRSYGSKNPGATNV
                     LRTGKKVAAVLTLAGDAGKGWLAVWLAQLYTGDPLAVALAGLAAFLGHLYPLFYRFQG
                     GKGVATAAGALFGFDVWLGLGTLVSWGVIAGFFRYASLASIVAALFAAFYAWWQFGAQ
                     PVSGAVLAMGALLIWRHRENIKRLAAGTESKLGAKKATPPDPSAASR"
     gene            2603..2956
                     /locus_tag="VGO02_10635"
     CDS             2603..2956
                     /locus_tag="VGO02_10635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEV7478179.1"
                     /translation="MDAILIRDLRIEASIGIHKRERHVKQTLSIDLDIGLPGEAVFRT
                     DKVADTIDYEQVTLRIGALIASGHFRLVETLADRIARLLLDEFGAPWTKVSVAKIGIL
                     PNAKFVGVTIERSRP"
BASE COUNT          513 a         1029 c          957 g          526 t
ORIGIN      
        1 gaggtccttg atggcgtcga cgtagcccat gccggcgtag gccatcagga aaccgatggc
       61 gttgccgtgc accccgcagc cgaagcagtg atagaactgc ttggacgggc tgacggtgaa
      121 cgagggggtt ttctcgccgt gaaaggggca cagccccatg aaattggcgc cgcccttctt
      181 gagctggacg tagcgctgca ccacgtccac gatgtcgacc cggttgagga gatcctgctt
      241 gaacgagtcg gggatcacgc cccgattttc agcccgaaag cttggctttg accagagaag
      301 aaaccgcgcc catgtcggcg cggccggcca ggcgcggctt gagaagcgcc atgaccttgc
      361 ccatgtcctt gattccagac gcaccggact cggcgagcgc cgcggcaacg gcctgcgcga
      421 gctccgcatc gcccatctgt tgcggcaggt aggccgagag cacgccgatc tcgaactttt
      481 ccgcctcggc cagatcgttg cgcgacgcct tctcgaactg cgcgatcgac tcgcggcgct
      541 gcttgatcat cttgtcgatc accgacagga catcggcgtc ggttagctgg atgcgttcgt
      601 cgacctctct ttgttttatt ccagacaaga tcaaccgaat tgccgacaga cgcgcgctgt
      661 ccctggcgcg catcgccgtc ttcatgtctt cggtgatttt gtcttttagc gaggtactaa
      721 tgcagttgct agtacatctt ctgcggcagc atctggctgc gcaggcgctt gtgatggcgc
      781 tttacggcgg cggcaagctt gcgcttgcgc tctgcggtcg gcttctcgta gtactcgcga
      841 gcgcgcagct cggtgagcag gcccgtcttc tccacggtgc gcttgaagcg gcgcatcgcc
      901 acttcgaacg gctcgttctc ttttactcgt acggtcggca tgactcccga aattccaggc
      961 tcgaaaaggg gctgaattgt atcagaatcg acccatgctg atcctcggca tcgagacctc
     1021 gtgcgacgaa accggcgtcg cactttatga cggcggcgca cgccggctgc tggcgcacgc
     1081 cgtgcactcc caagtggcga tgcacgaggc ctacggaggg gtggtacccg agctcgcctc
     1141 gcgcgaccac atccgccgcc tcgcccctct tacgcgcggg gttctggatt cctcgaacag
     1201 gcatttcagc gaattgagcg gcgtcgggta caccgaaggc ccgggcctgg ccggagcgct
     1261 cctcgtcggc gcctccttcg cccacggcct ggcggccgct ctgcacatcc cgtcggtggg
     1321 tattcaccac ctcgaggggc acctgctttc tcccctgctt tcagccgagc cgccggcctt
     1381 tccgttcgtc gcgctgctgg tttccggcgg ccacacccag ctcatgcggg tcgacggagt
     1441 cggccgctac gcgcttctgg gcgagaccca ggacgacgcc gcgggcgagg ccttcgacaa
     1501 gaccgccaag ctgctcggcc tgggctatcc gggcggcccg gcgctgtcga agctcgccga
     1561 gtcgggcgcg cccgggcgcc atcgcctgcc gcggcccatg ctcgacagcg ccgacctgga
     1621 tttcagcttc tccgggctga agacagccgt cctcactaca ttgaaaaaac accatcccga
     1681 ccctgcggac ctggcgcgcg ccttcgtcga tgcggtggtc gaggtgctgg ttgcgaagtg
     1741 tgtgcgcgct ctggacgaaa ccggcatggc gcgcctcgtg gtcgccggcg gcgtgggcgc
     1801 caaccggcag ctgcgcgccg cgctcaacgc cgaggcagcg cgccgcgggt tccgcgtttt
     1861 ctaccccgac gccgagctgt gcaccgacaa cggcgcgatg attgccttcg ccgcggcgct
     1921 gcacttcggg gaagccgccc tgaccggcgg ctttaccgtg aagccgcgct gggatctggc
     1981 ggcgtagcct tcttcgcgcc gagcttcgac tcggtgccgg cggcgaggcg tttgatgttc
     2041 tcgcggtgcc gccagatgag aagcgcgccc atcgccagca ccgcgccgct caccggctgc
     2101 gcgccaaact gccaccaggc atagaacgcc gcaaacaggg ccgcgacgat ggacgccagc
     2161 gacgcatagc ggaaaaaccc ggcgatcacc ccccagctca ccagggtacc gaggccgagc
     2221 cagacatcga agccgaataa cgcgcccgcg gcagtcgcca cgcccttgcc accctggaag
     2281 cggtagaaaa gcgggtagag gtggccaagg aaggctgcca gcccggcgag cgccaccgcc
     2341 aaaggatcgc cggtatagag ctgcgccaac cacaccgcaa gccagccctt gcccgcgtcg
     2401 ccggcgaggg tcagcacggc agccaccttc tttcccgtgc gtaacacatt ggtcgcgccc
     2461 gggttcttgg agccgtagct gcgcgggtcg ggcagccgca tcaggcggct gaccacaata
     2521 gcgaacgaga ccgaacccaa caggtacgcc gccagcacgg tcagcacggc ttgcatcgcg
     2581 cggattctat aatcggcccc taatggatgc cattctcatc cgggacctgc gcatcgaggc
     2641 atcgatcggc atccacaagc gcgagcgcca cgtgaagcag acgctgtcga tcgatctgga
     2701 catcggcctg ccgggggagg ccgtgttccg caccgacaag gtcgccgaca ccatcgacta
     2761 tgagcaggtc acgctcagga tcggcgcgct catcgccagc gggcatttcc gcctggtgga
     2821 gacgcttgcc gatcgaatcg cgcggctgct gctcgacgag ttcggcgcgc cgtggaccaa
     2881 ggtgagcgtg gccaaaatcg gcatcctgcc caacgccaag ttcgtcggcg tcaccattga
     2941 aagaagccgg ccctagtcct gcagcgcgac gtcgaccagc accggctgca gcgttcggac
     3001 gatttccccg ggtgagagct tgacc
//
LOCUS       DASYJA010000487         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1615578, whole genome shotgun sequence.
ACCESSION   DASYJA010000487 DASYJA010000000
VERSION     DASYJA010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615578"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VGO02_10640"
     CDS             <1..596
                     /locus_tag="VGO02_10640"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEV7478180.1"
                     /translation="PGDFFPVFEHYRMMPQLDRWVLRHTIKRLKAGSRIPQFTINLAA
                     QTIEDLEFPKFVAAELMGSGIAASGVAFEIDESDALTRPEACARFASAVKPIGCGVLL
                     DGFGRKSVTFEPLKVLRVDIVKIDGSLIRKLLGAEASLKKIQAIVRVADALKIGVVAE
                     CVEEQDLLVRLKALGVGYAQGFGVHQPMPMDRVAAPA"
     gene            complement(593..2260)
                     /locus_tag="VGO02_10645"
     CDS             complement(593..2260)
                     /locus_tag="VGO02_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEV7478181.1"
                     /translation="MGANDVYLAKPWLKHYPEGVPPTVEVPLKSLGQLFDEATARAPQ
                     KTAVAFYGRSITFRELREAADRLACALADLGVKKGDRVALYLLNSPQFIIAYFAALKC
                     GATVTSISPVYTSHEVRYQLQDSGARAIVCQDILYDKVAKSGAPMDLVVLTSIGEYLP
                     ALKRLFGKKAEVRPEKNLHWLQDLLKKYPATPPAVTIDPRKDLASLPYTGGTTGNPKG
                     VMLTHYNLVAAQAMGQAAFSVAIPGEEVILAFLPFFHIYGQVVIMLNGLCQGNLLVLF
                     TSPDTEEILTAMERYQATIFYGVPTLYEYLKDHKDTNKVDWRRLKLILSGADTLHEST
                     MKGWASRTGSSIVEGYGLSETCATSHVNPLQRPRVGSFGCPISSMNAAVIDAETMQFV
                     PQGTTGELVLAGPNVMQGYWNRPEETRRAFIEHSGMHWVRTGDVVRMDEEGYFHFYDR
                     SKDLIKYKGYSIFAKDVEDVLYGHPQVKAAGVIGVPDPAVGQRIKAIVVLQGDARGKV
                     SEDEIKAYCRQQLAEYKVPHVVEFRGELPKTDVGKVSRRELREETSA"
     gene            2290..>2573
                     /locus_tag="VGO02_10650"
     CDS             2290..>2573
                     /locus_tag="VGO02_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HEV7478182.1"
                     /translation="MDEIVARSMAKWPNVPAVYGWLSLDRRGSWRIKAERISNVALRE
                     FIARNYQADERGCWYFQNGPQRVFVELAYTPLVVHYEDEALLDHCARPFSS"
BASE COUNT          396 a          835 c          859 g          483 t
ORIGIN      
        1 cgccggggga tttcttcccg gtgttcgagc actaccggat gatgccgcag ctcgaccgct
       61 gggtgctgcg gcacaccatc aagcgcctga aggcaggttc gcgcatcccg cagttcacga
      121 tcaacctggc ggctcagacg atcgaggacc ttgagttccc gaagttcgtc gccgccgagc
      181 tgatgggctc gggcatcgcg gcgtcgggtg tcgccttcga gatcgacgag agcgacgcgc
      241 tcacgcggcc ggaagcctgc gctcgcttcg ccagcgcggt gaagccgatc ggctgcggcg
      301 tgctgctgga tggcttcggc cgcaagtccg tgaccttcga gccgctcaag gtgctgcgcg
      361 tggacatcgt gaagatcgac ggctccctga tccgcaagct gctcggcgcg gaggcgtcgc
      421 tcaagaagat ccaggcgatc gtgcgcgtcg ccgacgcgtt gaagatcggc gtcgtcgccg
      481 aatgcgtgga ggagcaggat ctgctggtgc gcctcaaggc tctcggggtc ggctacgcgc
      541 agggtttcgg cgtgcaccag ccgatgccga tggaccgcgt cgccgcgccc gcctaggcgc
      601 tggtttcttc cctcagctcg cggcgcgaca ccttgccgac gtcggtcttc ggcagctcac
      661 cgcggaactc cacgacgtgc ggcaccttgt actcggcgag ctgctgcctg cagtaggcct
      721 tgatctcgtc ctcgctcacc ttgccgcgcg cgtcgccctg cagcacgacg atcgccttga
      781 tgcgctggcc gaccgcgggg tcgggcacgc cgatcacccc cgcggctttc acctgaggat
      841 ggccgtagag cacgtcctcc acgtccttgg cgaagatcga gtagcccttg tacttgatca
      901 ggtccttcga gcggtcgtag aagtggaaat agccttcctc gtccatgcgc acgacgtcgc
      961 cggtgcgcac ccaatgcatg cccgaatgct cgatgaacgc gcgcctggtt tcctcggggc
     1021 ggttccagta gccctgcatc acgttcggcc cggccagcac cagctcgccg gtggtgccct
     1081 gcgggacgaa ctgcatcgtc tcggcatcga tcacggcggc gttcatgctc gagatcgggc
     1141 aaccgaaaga ccccacacgc gggcgctgca gcgggtttac gtggctcgtc gcgcaggtct
     1201 ccgacagacc gtagccttcg acgatgctcg agccggtgcg gctcgcccag cccttcatgg
     1261 tcgactcgtg cagcgtgtcc gcgcccgaaa ggatgagctt cagccgccgc cagtcgacct
     1321 tgttggtgtc cttgtggtcc ttcaggtact cgtagagggt cggcacgccg tagaagatgg
     1381 tcgcctggta gcgctccatc gccgtgagga tctcctcggt atcggggctg gtgaagagca
     1441 ccagcaggtt cccctggcac aggccgttca gcatgatgac gacctgcccg tagatgtgga
     1501 agaacggcag gaacgcgagg atcacctctt cgccggggat cgccaccgaa aacgcagcct
     1561 gccccatcgc ctgggcggcg accaggttgt agtgggtgag catcacgccc ttcggattgc
     1621 cggtcgtgcc gccggtgtag ggcagggacg cgagatcctt gcgcgggtcg atcgtcactg
     1681 ccggcggcgt ggccgggtat ttcttcagca ggtcctgcag ccagtggagg ttcttctcgg
     1741 ggcgcacctc ggccttcttg ccgaacaggc gcttcagcgc cggcaggtat tcgccgatgc
     1801 tggtgagcac caccaggtcc atgggcgctc ccgacttggc caccttgtcg tagaggatgt
     1861 cctggcacac gatcgcgcgc gccccgctgt cctgcagctg gtagcgcacc tcgtggctgg
     1921 tatagaccgg actgatcgag gtgaccgtgg cgccgcactt gagcgccgcg aagtaggcga
     1981 tgatgaattg cgggctgttg agcaggtaga gcgcgacgcg gtcgcccttc ttcacgccga
     2041 ggtcggcgag ggcgcaggca aggcggtcgg cggcctcgcg cagctcgcgg aaggtgatgg
     2101 aacggccata gaacgccacc gcggtttttt gcggagcgcg cgcggtggct tcatcgaaga
     2161 gctgtccgag cgacttcagc ggcacctcga ctgtcggcgg cacaccctcg gggtagtgct
     2221 tcagccaggg cttcgccagg tagacgtcgt ttgcgcccat gcctcctcct gcggatgatt
     2281 gtagcttcga tggacgagat cgtcgcccgc agcatggcca agtggcccaa tgtccccgcg
     2341 gtctacggct ggctgtccct cgatcggcgc ggcagctggc gcatcaaggc agagcggatc
     2401 tccaatgtgg cgctgcgcga attcatcgct cgcaactacc aggccgacga gcgcggctgc
     2461 tggtactttc agaacggccc gcagcgcgtt tttgtcgagc ttgcctacac gccgctggtg
     2521 gtgcattacg aggatgaagc gctgctcgac cactgcgcgc ggcctttttc atc
//
LOCUS       DASYJA010000488         2707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1616096, whole genome shotgun sequence.
ACCESSION   DASYJA010000488 DASYJA010000000
VERSION     DASYJA010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616096"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..740
                     /locus_tag="VGO02_10655"
     CDS             126..740
                     /locus_tag="VGO02_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV7478183.1"
                     /translation="MNDQAQIIEVVQNWALWRDAGDWERFRTVWHDDGWMTATWFQGP
                     AAEFIKVSKAGFDKGVSILHFLGGSSVDLKGSRAVAQTKMTISQRGDVDKEVVDVVCT
                     GRFFDFFEKRKEKWGIVRRQPIYEKDRMDPLDPSARLKLDPDLLGSFPEGYRHLAYLQ
                     TRLGFKIKQDLPQLKGPAVERLYRHGKAWLDGAPSPFPEEDRPR"
     gene            737..1333
                     /locus_tag="VGO02_10660"
     CDS             737..1333
                     /locus_tag="VGO02_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478184.1"
                     /translation="MNPLYQVLLTALLVFLLAGSLLGVAVGAGLLARSARAGAFMSAA
                     NHWVSTRRVLKPVELPRDTGHGGRGLGVALVAVGGYSLYVLAPLSIARVAAALRIDAS
                     STLALIGIEFAKWLLVAGCAVSVASGLMLLFFPQLWRALEERANRWYSSRQFTTGGDD
                     MHMPLDSLAGRHPRAAGAIVLALSLASAAATTLLLSRI"
     gene            1404..2636
                     /locus_tag="VGO02_10665"
     CDS             1404..2636
                     /locus_tag="VGO02_10665"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478185.1"
                     /translation="MCQWDSGPGGRVLVLGDYRQTVTVVRSLARAGYEVTLGTDHARS
                     ATTRSRYLSDVWIYEASCPKRFLDMLEAYLVAERPDFVFVVGESQLRHLAGATERFAP
                     LATWANAPFDVVARCFDKRAIYQLTPALGIPTLPWRGFDGGQDWPRAAAEMGYPVVVK
                     RKDSSAQVQERKALICATPAELDAFLGEVRADPDPASLVLQKFAAGKRHNCHVAADGG
                     ELIAYFQQKVIRTDELDDTGIGVAGVSVAPDPRLRAWCAGLVSAVGYSGIGCIQFLVD
                     ETSGEARFLEFNARMDSTAALPYQIGYDFPRFAMRIAGARRQGRRAPSSGAYYPPGKK
                     YHWLYGDISSWIDAARRGRRSPAALAGWALRIAWDACSSYHLTWDLLDPLPTLYQFSR
                     KFLAPALRRFQQPSLRHV"
BASE COUNT          400 a          963 c          914 g          430 t
ORIGIN      
        1 aaggagacct ggctcaagca gggcaccatc ccgatgggca tgtcgatcga gcagttcgac
       61 aagttcctgc gcgaagaaat cgtcaaatgg gcgaaggtcg tccaggtttc gggagccaag
      121 gcggattgaa cgaccaggcg caaataatcg aagtcgtgca gaactgggcg ctgtggcgcg
      181 acgccggcga ctgggagcgc ttccgcacgg tctggcacga cgatggctgg atgacggcca
      241 cctggttcca ggggccggcg gccgaattca tcaaggtgag caaggccggc ttcgacaagg
      301 gcgtgagcat cctgcatttc ctcggcggct cgagcgtcga cctgaagggc agccgggccg
      361 tggcgcagac caagatgacc atcagccagc gcggcgacgt cgacaaggag gtcgtggacg
      421 tggtctgcac cggccggttt ttcgatttct tcgaaaaaag aaaggaaaaa tggggcatcg
      481 tgcgccggca accgatctac gagaaggacc ggatggaccc gctggatccg tcggcgaggc
      541 tgaagctcga tcctgacctg ctgggctcct tcccggaagg ctaccgccac ctcgcctacc
      601 tgcagaccag gctcggcttc aagatcaagc aggatctgcc ccagctgaaa gggcccgcgg
      661 tcgagcggct gtaccgccac ggcaaggcct ggctcgacgg cgcgccatcg cccttccccg
      721 aagaggaccg gccgcgatga acccgctcta ccaggtcctg ctcaccgcgc tgctcgtgtt
      781 cctgctcgcc ggcagcctgc tcggcgtcgc cgtgggggcg ggcctgctcg cgcgcagcgc
      841 gcgggcgggc gctttcatga gcgcggcgaa tcactgggtc tcgacgcgcc gcgtcctcaa
      901 gccggtcgag ctgccgcgcg acaccggcca cggcggccgc ggcctcggcg tggcgctggt
      961 cgccgtcggc ggctactcgc tctacgtgct ggcgccgctg tccatcgcgc gcgtagccgc
     1021 ggcgctgcgc atcgatgcgt cctccacgct cgcgctgatc ggcatcgagt tcgcgaaatg
     1081 gctgctggta gccggctgcg ccgtctccgt agcgagcggc ctgatgctgc tgttctttcc
     1141 gcaactctgg cgcgctctcg aggagcgcgc caaccgctgg tattcctcgc gccagttcac
     1201 caccgggggc gacgacatgc atatgccgct cgacagcctg gccgggcgcc acccgcgcgc
     1261 cgcgggtgcg atcgtcctcg cgctctcgct cgcctcggcc gccgccacca ccctgctgct
     1321 ttcccgcatc tagtcctaac gggcggggcg cccggcgggg cgtgcggcaa gccgtgcaac
     1381 gtcgcccctg cagcatggcc cttatgtgcc agtgggattc gggtccgggc gggcgggttc
     1441 tcgtcctggg cgactatcgc cagacggtca ccgtggtgcg ctcgctcgcg cgcgcgggct
     1501 acgaagtcac gctcggcacc gaccatgcgc gcagcgccac cacgcgctcg cgctacctgt
     1561 cggacgtgtg gatctacgag gcttcctgcc cgaaacgctt cctcgacatg ctcgaggcct
     1621 acctcgtggc ggagcggccc gacttcgtgt tcgtggtggg cgaatcgcag ttgcgccacc
     1681 tggccggcgc caccgagcgc tttgcgccgc tcgccacctg ggcgaacgcg ccgttcgacg
     1741 tggtcgcgcg ctgcttcgac aagcgcgcga tatatcagct gacgccggcg ctcggcatcc
     1801 cgacgctgcc ttggcgtggc ttcgatggcg gccaggactg gccgcgcgcc gccgccgaga
     1861 tgggctaccc ggtggtggtc aagcgcaagg attcctcggc gcaggtgcag gagcgcaagg
     1921 cgctgatctg cgccacgccc gccgagctcg acgcgttcct cggcgaggtg cgcgccgacc
     1981 cggacccggc gtcgctcgtg ctgcagaagt tcgccgccgg caagcggcac aactgccacg
     2041 tggcggccga cggcggcgag ctgatcgcgt acttccagca gaaagtgatc cgcaccgacg
     2101 agctcgacga taccggcatc ggcgtggccg gcgtatcggt ggcgcccgac ccgcgcctgc
     2161 gcgcctggtg cgccgggctg gtgagcgccg tcggctacag cggcataggc tgcatccagt
     2221 tcctggtcga cgagacgagc ggcgaggcgc gcttcctcga attcaatgcg cgcatggaca
     2281 gcaccgcggc cctgccctac cagatcggct acgatttccc gcgcttcgcg atgcgtatcg
     2341 cgggcgcccg gcgccagggg cgccgcgccc cttcctccgg cgcctactac ccgccgggca
     2401 agaaatacca ttggctgtac ggcgatatct catcctggat cgacgcggcg cgccgcggcc
     2461 gccgcagccc ggcggcgctc gccggatggg cgctgcgcat cgcctgggac gcctgcagca
     2521 gctaccacct gacctgggac ctgctcgatc cgcttcccac gctctaccag ttctcgcgca
     2581 agttcctcgc cccggccctc aggcgcttcc agcaaccgtc gctgcgccac gtgtgaaccc
     2641 tgagaaggcg cgcaacatcg aggatctgcg ccgcctcgcg cgccgccgtc tgccgcgcat
     2701 cgtgcgc
//
LOCUS       DASYJA010000489         3241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2070889, whole genome shotgun sequence.
ACCESSION   DASYJA010000489 DASYJA010000000
VERSION     DASYJA010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2070889"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3241
                     /gene="rpoB"
                     /locus_tag="VGO02_10670"
     CDS             <1..>3241
                     /gene="rpoB"
                     /locus_tag="VGO02_10670"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV7478186.1"
                     /translation="TAARVAIYRMMRPGEPPTEDAVETLFNGLFYNEDRYDLSPVGRM
                     KFNRRAYPAKQDDRTPPWLRAFYQRVGPRDIEGLGTLQNDDILAVIGILVELRNGRGE
                     IDDIDHLGNRRVRSVGELAENQFRAGLVRVERAVKERLSQAESENLMPHDLINAKPIS
                     AAIKEFFGSSQLSQFMDQTNPLSEITHKRRVSALGPGGLTRERAGFEVRDVHPTHYGR
                     VCPIETPEGPNIGLINSLALYARTNKYGFLETPYRKVKDSKTTNEIVYLSAIEEGNYV
                     IAQANAQIDKNGKLVDTLVSCRFKNEFELKGPEEVQYMDVAPSQIVSVAASLIPFLEH
                     DDANRALMGSNMQRQAVPCLRPEKPLVGTGVERTAAIDSGTCVIALRGGIVDYVDANR
                     IVVRVNDDETVPGDVGVDIYKLTKYTRSNQNTNINQRPIVKQGEKIAKGDVVADGAST
                     DLGELALGQNMLVAFMPWNGFNFEDSILISERVVADDRFTSIHIEELTVVARDTKLGP
                     EEITRDISNLSEAQLARLDESGIVYIGAEVEAGDVLVGKVTPKGETQLTPEEKLLRAI
                     FGEKASDVKDTSLRVPSGISGTVIDVQVFTREGIERDKRSQSIIEDELKHYKTDLGDQ
                     MRIVESDTFERLERLLTGKAANGGPKRLAKGTKVAKDYLATVEKYDWFDIRLTNEEAA
                     AQVEGLKESIAQKRREFDAMFEVKRKKLTQGDELPPGVLKMVKVYVAVKRRLQPGDKM
                     AGRHGNKGVISKIVPEEDMPFLPDGTPVDIVLNPLGVPSRMNVGQILETHLGWAARIL
                     GFEAKTPVFRGADEAEIGALLRLAGFHWAAEGLRLRSRPPELTPQAITRMVQDLKKVV
                     PNGERTTLLEAGVAMLGSRSASQETRDLFQAVRDFMVDAAKELAERELAQRRQEVDFH
                     RARLEGDQAGRDDKAFTKALKEAEKLAGQSPTELLESIGKPALAGLLGPKGESNVDAA
                     TDELLRAAGLTPAGKAHLRDGRTGNPFEGEVTVGSIYMLKLSHLVDDKIHARSIGPYS
                     LVTQQPLAGKAQFGGQRFGEMEVWALEAYGAAHVLQEMLTVKSDDVNGR"
BASE COUNT          629 a         1045 c         1111 g          456 t
ORIGIN      
        1 aaaccgccgc ccgggttgcg atctaccgga tgatgcgccc cggcgagccg cccaccgaag
       61 acgcggtgga gacgctgttc aacggcctgt tctacaacga ggaccgctac gacctgtcgc
      121 cggtgggccg catgaagttc aaccgccgcg cctacccggc caagcaggat gaccgcaccc
      181 cgccgtggct gcgcgccttc taccagcgcg tcggtccgcg cgatatcgaa gggctgggca
      241 cgctgcagaa cgacgacatc ctggccgtca tcggcatcct ggtcgagctg cgcaacgggc
      301 gcggcgagat cgacgacatc gatcacctgg gcaaccggcg cgtgcgctcg gtgggcgagc
      361 tcgcggagaa ccagttccgc gcgggtctgg tgcgggtcga gcgcgccgtg aaggagcgcc
      421 tgtcgcaggc cgagtccgag aacctgatgc cgcacgacct gatcaacgcc aaaccgatct
      481 cggcggcgat caaggagttc ttcggatcca gtcagctgtc gcagttcatg gaccagacca
      541 accccttgtc cgagatcacg cacaagcggc gcgtctcggc gctcggaccg ggcggcctca
      601 cgcgcgagcg cgcgggcttc gaggtgcgcg acgtgcaccc gacgcactac ggccgtgtct
      661 gcccgatcga gacgccggaa ggcccgaaca tcggcctgat caactcgctc gcgctgtacg
      721 cacgcaccaa caagtacggc ttcctcgaga cgccctaccg caaggtcaag gactcgaaga
      781 ccaccaacga gatcgtctac ctgtcggcga tcgaggaggg caactacgtc atcgcccagg
      841 cgaacgcgca gatcgacaag aacggcaagc tggtcgacac cctggtgtcg tgccgcttca
      901 agaacgagtt cgagctgaag gggccggagg aagtgcagta catggacgtg gcgccctccc
      961 agatcgtgtc ggtggccgct tcgctgatcc cgttcctcga gcacgacgac gccaaccgcg
     1021 cgctgatggg ctccaacatg cagcgccagg cggtgccctg cctgcgcccg gagaagccgc
     1081 tggtcggcac cggcgtcgag cgcaccgcgg cgatcgactc gggtacctgc gtcatcgcgc
     1141 tgcgcggcgg gatcgtcgat tacgtcgacg ccaaccgcat cgtggtgcgc gtcaacgacg
     1201 acgagaccgt gccgggcgac gtcggcgtcg acatctacaa gctgacgaaa tacacgcgtt
     1261 ccaaccagaa caccaacatc aaccagcgcc cgatcgtgaa gcagggcgag aagatcgcca
     1321 agggcgacgt ggtggccgac ggcgcctcca ccgacctggg cgagctcgcg ctcggccaga
     1381 acatgctggt ggcgttcatg ccgtggaacg gcttcaactt cgaggactcg atcctgatca
     1441 gcgagcgggt cgtcgccgac gaccgcttca cctcgatcca catcgaggag ctgacggtgg
     1501 tcgcgcgcga caccaagctc gggccggaag agatcacgcg cgacatctcg aacctgtcgg
     1561 aggcgcagct ggcgcgcctc gacgagtcgg gcatcgtcta tatcggcgcc gaagtcgagg
     1621 cgggcgacgt cctggtcggc aaggtgacgc cgaagggcga gacccagctc acgccggaag
     1681 agaagctgct gcgcgccatc ttcggcgaga aggccagcga cgtgaaggac acctccctgc
     1741 gagtcccctc ggggatttcg ggcacggtga tcgacgtcca ggtattcacg cgcgaaggca
     1801 tcgagcgcga caagcgcagc cagtcgatca tcgaggacga gctcaaacac tacaagacgg
     1861 atctgggcga ccagatgagg atcgtggaga gcgatacctt cgagcgcctg gagcggctgc
     1921 tcaccggcaa ggccgccaac ggcggcccga agcgcctggc caagggcacc aaggtggcca
     1981 aggactacct cgccaccgtg gagaagtacg actggttcga catccgcctc accaacgagg
     2041 aggccgccgc gcaagtggaa ggcctgaagg agtcgatcgc gcagaaacgc agagaattcg
     2101 acgcgatgtt cgaggtcaag cgcaagaagc tgacccaggg cgacgagctg ccgccgggcg
     2161 tgctgaagat ggtcaaggtg tacgtcgcgg tgaagcgccg cctgcagccg ggcgacaaga
     2221 tggccggccg ccacggcaac aagggcgtga tctccaagat cgtgccggag gaggacatgc
     2281 cgtttctgcc cgacggcaca ccggtggaca tcgtgctcaa cccgctggga gtacccagcc
     2341 gaatgaacgt gggccagatc ctggagacgc acctgggctg ggccgcccgc atcctgggct
     2401 tcgaggccaa gacgccggtg ttccgcggcg ctgatgaggc cgagatcggg gcgctgctcc
     2461 gtctggccgg gttccactgg gcggctgagg gacttcgcct ccggagccgt ccgccggagc
     2521 tcacgccaca ggcgatcacc cggatggtcc aggatctcaa gaaggtggtg ccaaacggcg
     2581 agcgcactac gctgctcgag gccggggtcg ccatgctggg ctcccgcagc gcttcgcagg
     2641 agacccggga cctgttccaa gcggttcgcg acttcatggt cgatgccgcg aaggagctgg
     2701 ccgagcgcga gttggcgcag cggcgtcagg aggtggactt ccaccgcgct cgattggaag
     2761 gcgatcaggc tggccgcgat gataaggcgt tcaccaaggc gctgaaggag gccgaaaagc
     2821 tggccgggca gtcgccgacc gagctgctgg agtcgatcgg caagccggcg ttggccggtc
     2881 tgctcggacc caagggggag tcgaatgtgg acgcggcgac cgatgagctg ctgcgggccg
     2941 cgggcctcac cccggcgggc aaggcacacc tcagggacgg ccgcaccggt aatcccttcg
     3001 agggcgaggt cacggtcggc tcgatctaca tgctgaagct gagccacctg gtggacgata
     3061 agatccacgc ccggtcgatc ggcccgtact cgctggtcac ccagcagcca ctggccggca
     3121 aggcgcagtt cggtggacag agatttggtg agatggaggt ctgggcgctc gaggcctatg
     3181 gcgcggcgca cgtgctccag gaaatgctca cggtgaaatc ggatgacgtg aacggccgca
     3241 g
//
LOCUS       DASYJA010000490         2100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2797703, whole genome shotgun sequence.
ACCESSION   DASYJA010000490 DASYJA010000000
VERSION     DASYJA010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2797703"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /gene="arfB"
                     /locus_tag="VGO02_10675"
     CDS             complement(<1..229)
                     /gene="arfB"
                     /locus_tag="VGO02_10675"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEV7478187.1"
                     /translation="MRIPESEIEITAVRAQGAGGQNVNKVSNAVHLRFNVHASSLPEA
                     VKARLLARGDQRITRDGVVVIKAQQSRSLEKN"
     gene            complement(305..679)
                     /locus_tag="VGO02_10680"
     CDS             complement(305..679)
                     /locus_tag="VGO02_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478188.1"
                     /translation="MKKLLAIAVAGLAAAALALPGFAADDKADAKAGASADTKADVKA
                     DTKADTKADTKADVKADTGTSAGAGASTDAKTEKKAKKAKPKTAPSTSGSGSTSGSTS
                     SGSSAGATTATPSAPADAEKTK"
     gene            complement(853..1164)
                     /locus_tag="VGO02_10685"
     CDS             complement(853..1164)
                     /locus_tag="VGO02_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478189.1"
                     /translation="MKKVLQWVIAGLIAATLGLPALAADDEPKPKPKAKAKAKAKPKA
                     KADKADTKPDAKAVAPASMNERLRRGAENDQKDRARGTGASSGATSSTPSAPSGVDTT
                     R"
     gene            complement(1176..>2100)
                     /locus_tag="VGO02_10690"
     CDS             complement(1176..>2100)
                     /locus_tag="VGO02_10690"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEV7478190.1"
                     /translation="PLNALLAMFLPERGARHGAGLLWLALLALEAVLLAWVGRGALWD
                     ASELFDNWALRSPPTPLVGRLAFAAAFAAALCWRAWPDFTPLQVGNAGALAAFFIACE
                     WARLPNIFAAFMAAAGALLIAALLQESHRLAFRDELTGLPGRRALDERLRSLPGRYTV
                     AMVDVDHFKKFNDTHGHDIGDQVLKLVGARLAEVGGAGIAYRYGGEEFSVLFPHAELA
                     EALPHLEALRQSIEQYRMVVRSERRPKKAEVGALQRSDATQPLKTLSVTVSIGVAQPD
                     GAALRTPGQVLKSADEALYRAKQGGRNRVSR"
BASE COUNT          277 a          709 c          749 g          365 t
ORIGIN      
        1 ggttcttctc caggctgcgg ctctgctgcg ccttgatgac gacgacgccg tcgcgcgtga
       61 tgcgctggtc gccgcgcgcg agcaggcgcg cctttaccgc ttcgggcagc gaggaggcgt
      121 gcacgttgaa gcgcaggtgc accgcgttgg agaccttgtt gacgttctgc ccgccggcgc
      181 cctgcgcgcg caccgcggtg atctcgatct cgctctccgg gatccgcatt catcgaatgt
      241 aagcgaatcc ggacgcaaaa agggcgggca tatgcccgcc ctttgcgcca aagcaacggc
      301 tggattactt ggttttttcg gcgtccgccg gtgcagacgg ggtcgcggtc gtcgcgcccg
      361 cggagctgcc cgagctcgtg ctgccgctgg tcgagcccga gccgctggtg ctcggcgcgg
      421 tcttcggctt ggccttcttc gccttcttct ccgtcttggc gtcggtcgac gcgccggcgc
      481 cggcgctggt gccggtgtcg gccttgacgt cggccttggt gtcggccttg gtatcggctt
      541 tcgtatcggc cttcacatcg gccttggtgt cagcgcttgc gcccgccttg gcgtccgcct
      601 tgtcgtcggc ggcgaagccg ggaagggcga gggccgccgc ggcaagccct gcaactgcga
      661 tagcgagaag cttcttcatg ggtactgcct ccttggttga gttgatcgct cgggggaacc
      721 gagtatggag acagtccatt gcaacgctca tgccggggaa acccctacga cgtagtccta
      781 ccggatcaga attcggggtc agggtgcgaa ttccgaaaca aataatcgaa ttcgcaccct
      841 gaccccgaat tcctaccggg tcgtgtcgac gcccgacgga gccgacggtg tcgaggaagt
      901 cgcgccggag ctggcgccgg tgccgcgcgc gcgatccttc tggtcgttct ccgccccccg
      961 ccgcaggcgc tcgttcatgc tcgccggcgc aacggccttg gcgtctggtt tggtgtcggc
     1021 cttatcggcc ttggccttgg gcttcgcctt ggctttggct ttggctttgg gcttcggctt
     1081 gggctcgtcg tcggccgcga gggccggcag gccgagcgtg gcggcaatca accctgcgat
     1141 cacccactga agaaccttct tcatcgccct ctactctatc ggcttacgcg gtttcgtcca
     1201 ccttgcttgg cgcggtagag cgcctcgtcg gccgacttga gaacctggcc cggcgtgcgc
     1261 agcgcggcgc cgtcgggctg ggcgacgccg atcgagacgg tgaccgagag ggtcttcagg
     1321 ggctgcgtgg cgtcgctgcg ctgcagggcg ccgacctcgg ccttcttcgg ccgcctctcg
     1381 ctgcgcacca ccatgcggta ctgctcgatc gactggcgca gcgcctcgag gtgcggcagg
     1441 gcctcggcca gctcggcgtg cgggaagagc acgctgaact cctcgccgcc gtagcggtag
     1501 gcgatgccgg cgccgccgac ctcggcgagg cgcgcgccga ccagcttgag cacctggtcg
     1561 ccgatgtcgt ggccgtgggt gtcgttgaat ttcttgaaat ggtcgacgtc gaccatcgcc
     1621 accgtgtagc gcccggggag cgagcgcagc cgctcgtcga gcgcgcgccg ccccggcagg
     1681 ccggtgagct cgtcgcggaa ggcgaggcgg tgcgactcct gcaggagcgc cgcgatcagc
     1741 agcgcgccgg cggcggccat gaaggcggcg aagatgttcg gcagccgcgc ccactcgcag
     1801 gcgatgaaga aggcggcgag cgcgccggcg ttgccgacct gcagcggcgt gaaatcgggc
     1861 caggcgcgcc agcacagcgc ggcggcgaag gcggcggcga aggcgagccg gccgactagc
     1921 ggcgtcggcg gcgagcgcag cgcccagttg tcgaacagct cgctcgcgtc ccacagcgcg
     1981 ccgcgcccca cccaggcgag gagcaccgct tcgagcgcga gcagcgccag ccagagcagc
     2041 cccgcgccgt gccgcgcgcc gcgctcgggg aggaacatcg cgagcagcgc gttgagcggc
//
LOCUS       DASYJA010000491         3713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1344271, whole genome shotgun sequence.
ACCESSION   DASYJA010000491 DASYJA010000000
VERSION     DASYJA010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344271"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2022
                     /locus_tag="VGO02_10695"
     CDS             <1..2022
                     /locus_tag="VGO02_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family V/XII"
                     /protein_id="HEV7478191.1"
                     /translation="YQPQLERWADNNLSGRAAVAVTRPGEKEPRYGVIELTARTQVDK
                     PADLVTLSAVRIVKSSFPGATPEEAERYLATLRAAVTKTEWPVSAQALQANLAIAQTQ
                     SKQKALPVKNDPPQILFRTVPSLLALVDGEPTLRDAKDATGLKRVANMSALMLLEESS
                     ATYYLWALERWWQSKAVGGEWQAAPAPPASLERARAALGKEFNPLDGKDADGKPVFEP
                     GITPQIIVATRPTELLQSLGEPKLTPIPGTQLLYVANSRNDILMDLGGQAYYALISGR
                     WFTAKALTGPWSFASAKSLPADFARIPAGHPMGDVRMSVAGTPEAREAAIANTIPQTA
                     TVQRDIKPTSVNYDAGPPQWKPIEGTPLSYAFNAPAAVIRVDPKTYYMVQNGVWFTGP
                     SPFSWSVATTVPAVIYTIPANSPMHYVTYVRVYGSTPTVVYVGYTPGYYGTVLSSDGV
                     VVYGTGYVYPYYVGPYYYYPPPYYTYGYGAGFTVGLFFGFAMGGGWHGPCCYGGGGGV
                     YISHHNNININNSYNRWGGKSTSISGPGGREVKASQVGNTTFAKGSGSNNVYAGRDGN
                     VYKNEGGDWQKYGGKGEGWSDLDKPQRGERPSQQPAGGAGTRDRPEQSLPGETRSSLD
                     RQQQSRDVGTQRANDVRQSGGGGGGFQGGGGARGGGFGGGGGRGGGGRR"
     gene            2025..2870
                     /locus_tag="VGO02_10700"
     CDS             2025..2870
                     /locus_tag="VGO02_10700"
                     /inference="COORDINATES: protein motif:HMM:NF014324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEV7478192.1"
                     /translation="MAFGRLFLLLAALPGAAAAQDAGIWEKRIEADARREVFTLTSRK
                     PNYILVSYMKEPNQAPYQFTGSADRLENQEVKFQLSFQTKMADDLFGGNGDLWFGYTQ
                     VSWWQLFNSAISAPFRETNYEPEVYVSFLTGYELLGLKLRAVNVGAVHQSNGRAEPLS
                     RSWNRVFADFQLVRGDFALGFKPWARIKEDPQHDDNPDITDYLGNYELRAFYHWRGQV
                     FTGMVRNLFDSEHRYNAELGWSFPIAGKLRGMVQWYKGYGESLIDYNFKMNRIGVGVL
                     LTDWL"
     gene            2867..>3713
                     /locus_tag="VGO02_10705"
     CDS             2867..>3713
                     /locus_tag="VGO02_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV7478193.1"
                     /translation="MKLLLCIVLLIPWQALAQTDEKKSPFTDPQDGKRDASEWLLERK
                     GFLPVPIIITEPAIGYGAGAALLFFRESIGERAAQAKETGRLVPPDIYGVALAATENG
                     TKVGGAFGMVTFAQENWRWRGGVARPDVNLDFYGTNGTDSPREFKLGYNLDGWISTQQ
                     LMRRLGDSENFIGARWIWLDLDTTFDPARSAPLPGLGGRTVRSSGVGPLFEHDSRDNI
                     FTPSRGWKGYLEALFYSPDIGSDNKYETYRAYAFGYTPLWKEFVLGGRLDSRAARGDV
                     PFYQLP"
BASE COUNT          629 a         1299 c         1242 g          543 t
ORIGIN      
        1 taccagccgc agctcgagcg ctgggccgac aacaacctct cgggccgcgc cgccgtggcg
       61 gtgacgcgcc ccggcgagaa ggaaccgcgc tacggagtga tcgagctcac cgcgcgcacg
      121 caggtcgaca agcccgcgga cctggtcacg ctcagcgcgg tgcgcatcgt gaaaagcagc
      181 tttcccggcg cgaccccgga ggaggcggag cgctacctcg ccaccctgcg cgccgcggtg
      241 accaagaccg agtggccggt ctcggcgcag gcgctgcagg ccaacctcgc catcgcccag
      301 acgcagtcga agcagaaggc gctgccggtg aagaacgacc cgccgcagat cctgttccgc
      361 accgtgccct cgcttctcgc gctggtcgac ggcgagccga cgctgcgcga cgcgaaggac
      421 gcgaccggcc tgaagcgcgt cgccaacatg tccgcgctga tgctgctgga ggaatcgagc
      481 gccacgtact acctgtgggc gctcgagcgc tggtggcagt cgaaggccgt cggcggcgaa
      541 tggcaggccg cgcccgcgcc gcccgcgagc ctcgagcgcg cgcgcgcggc gctcggcaag
      601 gagttcaacc cgctcgacgg caaggacgcc gacggcaagc cggtgttcga gccgggcatc
      661 acgccgcaga tcatcgtcgc cacgcggccg accgagctgc tgcagtcgct gggtgagccg
      721 aagctcacgc cgatcccggg cacgcagctc ctctacgtgg cgaattcgcg caacgacatc
      781 ctgatggacc tgggcgggca ggcctactac gcgctgatct cgggccgctg gttcacggcg
      841 aaggcgctga cggggccatg gagcttcgcc tcggccaagt cgctgcccgc cgacttcgcc
      901 aggatcccgg ccggccatcc gatgggcgac gtgcgcatgt cggtggcggg tacgcccgag
      961 gcgcgcgagg cggcgatcgc caacaccatc ccgcagaccg ccaccgtgca gcgcgacatc
     1021 aagccgacga gcgtgaacta cgacgccggc ccgccgcaat ggaagccgat cgagggcacg
     1081 ccgctcagct acgccttcaa cgccccggcg gcagtgatcc gcgtcgaccc caagacgtac
     1141 tacatggtgc agaacggcgt ctggtttacc ggcccctcgc cgttttcctg gagcgtcgca
     1201 acgacggtgc ccgcggtgat ctacacgatc ccggcgaact cgccgatgca ctacgtcacg
     1261 tacgtgcgcg tctatggcag cacgcccacg gtcgtgtacg ttggctatac gcccggctac
     1321 tacggcacgg tgctgtcgtc cgacggcgta gtggtctacg gcactggcta tgtctatccg
     1381 tactatgtcg gtccctacta ttactaccca ccgccgtatt acacatacgg ctacggcgcc
     1441 ggtttcaccg tcggcttgtt cttcggcttc gccatgggcg gtggctggca cggcccctgc
     1501 tgctacggcg gcggtggtgg cgtctacatc agccaccaca acaacatcaa catcaacaac
     1561 agctacaacc ggtggggcgg caagagcacg agcatcagcg gaccgggcgg gcgcgaggtg
     1621 aaggcctcgc aggtcggcaa caccaccttc gccaagggct cgggcagcaa caacgtctac
     1681 gccgggcgcg acggcaacgt ctacaagaac gaaggcggcg actggcagaa atacggcggc
     1741 aagggcgagg gctggtcgga tctcgacaaa ccgcagcgcg gcgagcgtcc gtcgcagcag
     1801 cccgccggcg gcgccggcac gcgcgaccgg ccggagcaga gcctccccgg cgagacgcgc
     1861 tcctcgctcg accggcagca gcagtcgcgc gacgttggca cgcagcgcgc gaacgatgtc
     1921 cgccagtccg gcggcggagg cggcggcttc cagggcggcg gcggtgcccg cggcggcgga
     1981 ttcggcggcg gcggcggccg cggcggcggc ggccgcagat agccttggcc ttcggcagac
     2041 tgtttctgct actggcggcg ctgcccggcg ccgcggcggc gcaggacgcg ggcatctggg
     2101 agaagcgcat cgaggcggac gcgcggcgcg aggtcttcac gctcaccagc cgcaagccca
     2161 actacatcct ggtgagctac atgaaggagc ccaaccaggc gccctaccag ttcaccggat
     2221 ccgccgaccg gctggagaac caggaggtca agttccagct cagcttccag accaagatgg
     2281 ccgacgatct gttcggcggc aatggcgacc tttggttcgg ctatacgcag gtctcatggt
     2341 ggcaactctt caacagcgcg atctcggcgc ctttccgcga gacgaactac gagcccgagg
     2401 tctacgtctc gttcctgacc ggctatgagc tccttggtct caagctgcgc gccgtcaatg
     2461 tgggcgcggt ccaccagtcg aacggccgcg ccgagccgct gtctcgcagc tggaaccgcg
     2521 tgttcgccga cttccagctg gtgcgcggcg acttcgcgct gggcttcaag ccctgggcgc
     2581 gcatcaagga ggacccgcag cacgacgaca atcccgacat cacggactac ctcggcaact
     2641 acgagctgcg cgccttctat cactggaggg gccaggtgtt caccggcatg gtgcgcaacc
     2701 tcttcgacag cgagcaccgg tacaacgcgg agctcggctg gtcgtttccg atcgccggca
     2761 agctgcgcgg catggtgcag tggtacaagg gctacggcga aagcctgatc gactataact
     2821 tcaagatgaa ccgcatcggg gtcggcgtgc tgctcaccga ttggctgtga agctccttct
     2881 ctgcatcgtt ctcctgattc cgtggcaggc gctcgcgcag accgacgaga agaagagccc
     2941 gttcaccgac ccccaggacg gcaagcgcga cgcctcggag tggctgctcg aaaggaaggg
     3001 cttcctgccg gtgccgatca tcatcaccga gccggccatc ggctatggcg cgggcgcggc
     3061 gctgctcttc ttccgcgagt cgatcggcga gcgcgcggcg caggcgaagg agaccggccg
     3121 cctcgtgccg cccgacatct acggcgtcgc gctggcggcg acggagaacg gcaccaaggt
     3181 gggcggcgct ttcggcatgg tgaccttcgc gcaggagaac tggcgctggc gcggcggcgt
     3241 ggcgcgcccc gacgtgaacc tcgacttcta cggcaccaac ggcaccgaca gcccgcgcga
     3301 gttcaagctc ggctacaacc tcgacggctg gatctcgacg cagcagctca tgcgccgcct
     3361 cggtgacagc gagaatttca tcggcgctcg ctggatctgg ctcgacctcg acaccacctt
     3421 cgaccccgcg cgctccgctc cgctgcccgg cctgggcggg cgcaccgtgc gcagctccgg
     3481 cgtcggcccg ctcttcgagc acgactcgcg cgacaacatc ttcacgccgt cgcgcggctg
     3541 gaagggctac ctggaggcgc tgttctattc gccggacatc ggcagcgaca acaagtacga
     3601 aacgtaccgc gcgtacgcct tcggctacac gccgctgtgg aaggagttcg tgctcggcgg
     3661 ccggctcgat tcgcgcgcgg cgcgcggcga cgtgccgttc taccagctgc cct
//
LOCUS       DASYJA010000492         2932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1254412, whole genome shotgun sequence.
ACCESSION   DASYJA010000492 DASYJA010000000
VERSION     DASYJA010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254412"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1973)
                     /locus_tag="VGO02_10710"
     CDS             complement(<1..1973)
                     /locus_tag="VGO02_10710"
                     /inference="COORDINATES: protein motif:HMM:NF027656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4962 domain-containing protein"
                     /protein_id="HEV7478194.1"
                     /translation="MLRVIGFLALFPVAAALAGGISAECTHDAPPDWMAVSDPHAAQV
                     RPADCAAVEQTPPDFSWPDIARDARYEVTLIYPGGRKKTLAAPQNYLNWNEVLPAGEY
                     SWQVKAVSTRGTQESRVRKFLVSEKSKPFLLPDMKELSATLAAKSHPRGLPDAQTLKK
                     MADQRSAGLATLLREVNGKTRNPLPGEPNHPNPGTNDASVFDEVKRTLNSLQAYALTR
                     EERYYADALRRVRNLASWDPKGKTAFTERGMDRSARFLTFTLALGYDWLHPRLDEATK
                     SQLTAVLKVRMGQMYGDLVGERSRIAAQPRDSHGIQTSIYLAAMLPLVAGDVAGADAW
                     MARALPLALSLISPWAGEEGGFANGSAYSLWGMGDALLYFYVLRWTTGIDVAQKPWVR
                     NWGRYMAYFDPPGSPARLFGDGHEQAALNEARARYGKGYAAFSPTPLGQWWASRLSGE
                     DASRAEFLLSPLNENRKAALPEGTPNSLYLPSIGWVAMHSSLADDARSSVYFKSAPPP
                     FGAFNHSHADQNGFVVNAGGQRLAIESGYYDAYKSAHWMKWLHQTRSKNAITYDGGKG
                     QLFFEQDDKEGYGGVTRFASAAGYDIVSGDATRAYGGAVSAALRTLVYLRPDVVVVYD
                     RLASSTPRQWDWNIHALNQMERSGKSITIRNGG"
     gene            2163..2792
                     /locus_tag="VGO02_10715"
     CDS             2163..2792
                     /locus_tag="VGO02_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7478195.1"
                     /translation="MRILIADDHPIVRHGLKQVLANDPAATVVGEATNGDEALELARK
                     VEWDMAIFDFSMPGRSGLDLLGDLKREFPGRPVLILSMHSEDVHGSRALRGGAAGYIT
                     KESAPTELTTAVRKVANGGKYVSSSLAEILASECSSDPQKALHEKLSDREYRVMWLLA
                     TGKQISEIAKEMGLSPSTVSTYRTRILRKLSLTSNAALVHYAVRHQLVG"
BASE COUNT          470 a          968 c          999 g          495 t
ORIGIN      
        1 tggccgccgt tcctgatggt gatgctcttg cccgagcgtt ccatctggtt gagcgcgtgg
       61 atgttccagt cccactgccg cggcgtgctc gacgccagcc ggtcgtaaac gacgaccaca
      121 tccggacgca agtacacgag ggtgcgcagc gcagcgctca cggcgccgcc gtaggcacgc
      181 gtcgcatcgc cgctcacgat gtcgtagccc gcggccgagg cgaagcgcgt cacgccgccg
      241 tagccttcct tgtcgtcctg ctcgaagaac agctgcccct tgccgccgtc gtaggtgatg
      301 gcgttcttcg agcgcgtctg gtgcagccat ttcatccagt gcgccgactt gtaggcgtcg
      361 tagtagccgc tctcgatcgc gaggcgctgc ccgccggcgt tgaccacgaa gccgttctgg
      421 tcggcgtgcg aatggttgaa ggcgccgaag ggcggcggcg cggatttgaa gtacacggag
      481 ctgcgagcgt cgtcggcgag gctcgagtgc atcgccaccc agccgatcga gggcaggtac
      541 agggaatttg gggtgccttc cggtagcgcg gccttgcggt tttcattcaa tggcgaaagc
      601 agaaattcgg cgcggctcgc gtcctcgccc gacaggcgcg atgcccacca ttgtccgagc
      661 ggcgtgggcg agaaggccgc gtagcccttg ccgtagcgcg cgcgcgcctc gttgagcgcc
      721 gcctgctcgt ggccgtcgcc gaacaggcgc gcgggcgagc ccggcgggtc gaagtacgcc
      781 atatagcggc cccagttgcg cacccagggc ttctgcgcca cgtcgatgcc ggtggtccag
      841 cgcagcacat agaagtagag cagcgcgtcg cccatgcccc agaggctgta ggccgagccg
      901 ttggcgaagc cgccttcctc gccggcccac gggctgatca ggctgagcgc gagcggcagt
      961 gcgcgcgcca tccacgcgtc cgccccggcg acgtcgccgg ccaccagcgg cagcatcgcg
     1021 gcgagataga tcgaggtctg gatgccgtgc gagtcgcgcg gctgcgcggc gatgcgcgag
     1081 cgctcgccta ccaggtcgcc gtacatctgg cccatgcgca ccttgagcac cgcggtaagc
     1141 tggcttttgg tcgcctcgtc gaggcgcggg tgcagccagt cgtagccgag cgccagcgtg
     1201 aaggtcagga agcgcgcgct gcggtccatg ccgcgctcgg tgaaggcggt cttgcctttc
     1261 gggtcccacg aggcgaggtt gcgcacgcgg cgcagcgcgt cggcgtagta gcgctcctcc
     1321 ctggtcagcg cataggcctg cagcgagttg agggtgcgct tcacctcgtc gaacacgctg
     1381 gcgtcgttgg tgccgggatt ggggtggttc ggctcgcccg gcagcgggtt gcgggtcttg
     1441 ccgttgacct cgcgcagcag agtggcgagg cccgcggaac gctggtcggc catcttcttc
     1501 agcgtctgcg cgtcgggcag gccgcgcggg tgggacttcg ctgcgagcgt ggcggaaagc
     1561 tccttcatat ccggcagcag gaatggcttg ctcttctcag aaaccaggaa cttgcgcacg
     1621 cggctctcct gcgtgccgcg cgtgctgacc gctttcacct gccacgagta ttcgccagcc
     1681 ggcagcacct cgttccagtt gaggtagttc tgcggcgcgg cgagtgtctt ctttcggccg
     1741 ccgggataaa tgagcgtcac ctcatagcgc gcgtcgcgcg cgatgtcggg ccagctgaaa
     1801 tcgggcggcg tctgctcgac cgcggcgcaa tcggccggcc gcacctgggc cgcgtgggga
     1861 tcgctcaccg ccatccagtc gggcggcgcg tcgtgcgtgc attcggctga gatgccgccg
     1921 gccagcgctg ccgccacagg aaaaagcgca agaaatccga tgactcggag cacggcggaa
     1981 gcattgcgcc gcgggccgag cgcatctatc gcgcggcgtc ccccgcaagg actaaattgt
     2041 gcttgtcggg caaataccta cacgcctgtc gggaacgcgc tacatgattg ttctattgcg
     2101 gaaaagggat ggctaattgc ttcgcgcatg cctatacttt ttcgcgttcc agggagatac
     2161 ccatgcgaat tctgatagct gacgatcacc cgatagtccg tcacggactc aagcaggttc
     2221 tcgccaacga tcccgccgcc actgtagtcg gcgaggcgac taacggcgac gaggcgctcg
     2281 agctggcgcg caaggtcgaa tgggacatgg cgatcttcga tttctccatg cccgggcgca
     2341 gcggcctcga cctgctcggc gacctgaagc gcgaattccc gggccgcccg gtgctgatcc
     2401 tcagcatgca ttccgaggac gtgcacggct cgcgcgcgct gcgcggcggc gccgccggct
     2461 acatcacgaa ggaaagcgcg ccgacggagc tcaccaccgc ggtgcgcaag gtggcgaacg
     2521 gcggcaagta cgtcagctcg tcgctggccg agatcctcgc ctccgagtgc tcgagcgatc
     2581 cgcagaaagc gctgcacgag aagctctccg accgcgagta ccgcgtcatg tggctgctgg
     2641 cgacaggcaa gcagataagt gaaattgcga aggagatggg cctgtctccc tcgacggtaa
     2701 gcacctaccg cacgcgcatc ctgcgcaagc tcagcctgac cagcaacgcg gcgctggtgc
     2761 actacgccgt gcgccaccag ctggtcggat gaccgatttg tcgcaacctg cgctgacgca
     2821 tcgccaactg gcggccgcgg aacgcggcac gcctgcccag tccgccacgc gggcgaagat
     2881 cctgctcgtc gacgacgagc cgaagagcct gtacgcgctg cagcagctgc tc
//
LOCUS       DASYJA010000493         1790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_529795, whole genome shotgun sequence.
ACCESSION   DASYJA010000493 DASYJA010000000
VERSION     DASYJA010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529795"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VGO02_10720"
     CDS             <1..693
                     /locus_tag="VGO02_10720"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEV7478196.1"
                     /translation="LGMKSSKFMNASGLPDAQHYTTAQDLYTLASALIRDFPAEYGQY
                     YSQKEFRYNNITQPNRNRLLWIDPSVDGVKTGHTEAAGYCLVASSRRGERRLVSVLLG
                     STSEATRAQESLKLLNWGFQFFDSVRLYKSGEAVKTLEVWKGTAKAVKAGLKSDLLVA
                     VPKGEAEKIKAELVAQQPLVAPLAKDQRIGVLRVSFDGKPVGEYPLLALEPVAEAGIF
                     GRAWDTLRLWFK"
     gene            696..1565
                     /locus_tag="VGO02_10725"
     CDS             696..1565
                     /locus_tag="VGO02_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HEV7478197.1"
                     /translation="MVFLNGKLLPVEQATVSVLDRGFIFGDGVYELVPVYSRVPFRLE
                     EHLARLERSLGEVKIRNPYSRAQWRGHVHGLIDAQPFDDQGVYFQVTRGAAKRDHAFP
                     KSIEPTVFMMANPLVNPPQAQVERGGAAVSAPDNRWLRCDIKSISLVGNVLLRQLSAE
                     ADAAETILFRDGKLTEASASNVFVVKGGVIQSPPKSNLILPGITYDVIAELAAANALP
                     MEFRDVTEAEVRGADEVWVSSSSKEVFPIVTLDGRQIGDGKPGPMFRRMYQLYQEFKQ
                     KVMRAGKREAVSA"
     gene            1558..>1790
                     /locus_tag="VGO02_10730"
     CDS             1558..>1790
                     /locus_tag="VGO02_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HEV7478198.1"
                     /translation="MPESSLAFPCDFPIKIMGRKERGFAQSMTEIVLKHAPEFDVASV
                     EMRPSRQDKYLSITCVVRAVSREQLDSLYQELC"
BASE COUNT          329 a          577 c          586 g          298 t
ORIGIN      
        1 ctcggcatga agtcgagcaa attcatgaac gcctcgggac tgcccgacgc gcagcactac
       61 accaccgcgc aggacctcta caccctcgcc agcgcgctga tccgcgactt tcctgccgag
      121 tacggccagt actattcgca gaaggaattc cgctacaaca acatcacgca gcccaaccgc
      181 aaccggctgc tgtggatcga cccgagcgtg gacggcgtca aaaccggcca taccgaggcc
      241 gcgggctact gcctcgtcgc ctcctcgcgg cgcggcgagc gccgcctggt ttccgtgctc
      301 ctcggctcga cctcggaagc aacgcgcgcg caggaatcgc tcaagctgct gaactggggc
      361 ttccagtttt tcgacagcgt gcgcctctac aagagcggcg aggcggtgaa gaccctcgag
      421 gtatggaagg gcacggcaaa ggcggtcaag gccggcctga agagcgatct gctggtggcg
      481 gtgcccaagg gcgaggcgga aaagatcaag gccgagctcg tcgcgcagca gccgctggtc
      541 gcgccgctcg cgaaggacca gcgcatcggc gtgctgcgcg tgagcttcga cggcaagccg
      601 gtcggcgaat atccgctgct cgctctcgag ccggtggccg aggcgggcat tttcggccgc
      661 gcctgggata cactcaggct gtggttcaag taaacatggt ttttttgaac ggaaagcttc
      721 ttcccgtcga gcaagcgacc gtctcggtgc tcgaccgcgg tttcatcttc ggcgacggcg
      781 tctacgagct ggtaccggtg tactcgcggg tgccgttccg cctcgaggag cacctggcgc
      841 gcctcgagcg cagcctgggc gaagtgaaaa tccgcaaccc gtattcgcgc gcgcagtggc
      901 gcgggcatgt tcacggcctg atcgacgctc agccgttcga cgaccagggc gtctatttcc
      961 aggtgacgcg cggcgcggcg aagcgcgacc acgcctttcc gaagagcatc gagcccaccg
     1021 tgttcatgat ggccaacccg ctggtcaacc cgccgcaggc gcaggtcgag cgcggcggcg
     1081 cggcggtgag cgcgccggac aaccgctggc tgcgctgcga catcaagtcg atctcgctcg
     1141 tcggcaacgt gctgctgcgc cagctctcgg ccgaggccga cgcggccgag acgatcctgt
     1201 tccgcgacgg caagctcacc gaggcgtccg cctcgaacgt gttcgtcgtc aagggcggcg
     1261 tgatccagtc gccgccgaag tcgaacctga tcctgccggg catcacctac gacgtgatcg
     1321 ccgagctagc cgccgccaac gctctgccga tggaattcag agatgtaacc gaggccgaag
     1381 tgcgcggcgc ggacgaggtt tgggtcagct cctcatcgaa ggaagtgttt cctatcgtga
     1441 cgctggacgg ccggcagata ggcgacggca agccggggcc gatgttccgc cgcatgtacc
     1501 agctctacca ggagttcaag cagaaggtca tgcgcgcggg caagagagag gcggtttctg
     1561 cctgagtcgt cgttagcttt tccctgcgac ttcccgatca agatcatggg caggaaagag
     1621 cggggtttcg cacagagcat gaccgaaatc gtcctcaagc acgcgccgga gttcgatgtg
     1681 gccagcgtgg aaatgcgtcc cagccggcag gacaagtacc tgagcatcac ctgcgtggtg
     1741 cgcgccgtct cgcgcgagca gctcgattcg ctctaccagg agctgtgcga
//
LOCUS       DASYJA010000494         3278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1074441, whole genome shotgun sequence.
ACCESSION   DASYJA010000494 DASYJA010000000
VERSION     DASYJA010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074441"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VGO02_10735"
     CDS             complement(<1..429)
                     /locus_tag="VGO02_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEV7478199.1"
                     /translation="MCGIVGAAARRDIVPVLIEGLKRLEYRGYDSCGVAVLNGAGIGR
                     VRSVERVADLAKQIRSKKVRGVTGIAHTRWATHGAPVTKNAHPMMSRGEIAVVHNGII
                     ENYEELRAELKAKGYAFDSQTDTEVIAHLVHSHYDGDLSLA"
     gene            complement(434..1804)
                     /gene="glmU"
                     /locus_tag="VGO02_10740"
     CDS             complement(434..1804)
                     /gene="glmU"
                     /locus_tag="VGO02_10740"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEV7478200.1"
                     /translation="MPLDVIVLAAGQGKRMHSALPKVLHALAGRPLLAHVLDAARALK
                     PRKVVVVHGHGGDEVRAAFPDPAIEWVAQTEQLGTGHAVRQAMPQVAADAEVLILYGD
                     VPLVRAETLKRLLDASQRGVAVLTAEVANPTGYGRIVRASAGRVSRIVEQKDANPAEA
                     AIREINAGFFALRASHLSAWLAKITNQNAQQEYYLTDIVSLAGADDIPVFAVKVEDRW
                     EVAGVNSKQDLAGLERVVQHREAARLLEGGVTLADPARIDVRGSLECGRDVSIDVNCV
                     FEGKVTLGDHVRIGPNCVLRNVSIAAGTEVRAFSHLDEAEVGARCIVGPYARLRPGAS
                     LADEAHIGNFVEVKASRVGKGSKANHLAYIGDSEVGANVNIGAGTITCNYDGANKNRT
                     VIEDDCFIGSDATLVAPLRLAKGSYIGAGSTITKNTPAGQLTVARAKQVSIPSWKPPK
                     KAKKKD"
     gene            1845..2861
                     /gene="phnD"
                     /locus_tag="VGO02_10745"
     CDS             1845..2861
                     /gene="phnD"
                     /locus_tag="VGO02_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV7478201.1"
                     /translation="MNIVTACAGIMLSLGTVQAFAQDSCANRGELDALYCDENRDLVA
                     DTPKDPKKLKNPSTLVFTYTPVEDPAVYENVFKPFTDYLSKCTAKRVVFYQVQSNAAE
                     IEAMRSGRLHVGGFSTGPTNYAVNLAGAVPFAVKGTATELQGYQLIFVVRKDSPYQKL
                     ADLKGKKVAHVQPSSNSGNLAPRVLFPKEGLVPDKDYKVLYSGKHDQTVMGVLSGDFD
                     GGPVASDVFRRMGERGQIKTDDFRIIYRSQTFPTSSFAYAHDLEPKLRDTMLKCFYDY
                     RFTPEMQKAFDGADRFYPITYKEHWAVVRHVAEGSGEKFNKAAFDNLQKAEAEAAAKK
                     AQKK"
     gene            2909..>3278
                     /locus_tag="VGO02_10750"
     CDS             2909..>3278
                     /locus_tag="VGO02_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7478202.1"
                     /translation="MLRGIDLEFGAQGMTAVIGPSGTGKSTLLRCINRLVEPTSGEVI
                     FEDRDVTRLSRGELRLVRRRIGMVFQEYNLVERLTVMENLLCGRLGYVSPFKAWLRRY
                     PPRDIARAFELLETVGLAAHA"
BASE COUNT          527 a         1120 c         1079 g          552 t
ORIGIN      
        1 cgccagcgac aggtcaccgt cgtagtggct gtggacgagg tgcgcaatga cttcggtgtc
       61 ggtctggctg tcgaaggcat agcccttggc cttgagctcg gcgcgcagct cctcgtagtt
      121 ctcgatgatg ccgttgtgca ccaccgcgat ctcgccgcgc gacatcatcg gatgcgcgtt
      181 cttcgtcacc ggcgcgccgt gcgtcgccca gcgggtgtgc gcgatgccgg tcacgccccg
      241 gaccttcttg cttcttatct gcttggcgag gtccgcgacg cgctcgacgc tgcggacacg
      301 gccaatgccg gcaccgttca ggacggcaac gccgcacgag tcatagccgc ggtactcgag
      361 ccgcttcagg ccctcgatca ggaccgggac gatgtcgcgc cttgcggccg cgccgacgat
      421 gccgcacatg gtgtcagtcc ttcttcttcg cctttttcgg cggcttccac gacgggatcg
      481 acacctgctt cgcgcgcgcc acagtgagct ggccggccgg cgtgttcttg gtgatggtcg
      541 agccggcgcc gatgtaggag cccttggcga ggcgcagcgg ggcgaccagc gtggcgtccg
      601 agccgatgaa gcagtcgtcc tcgatcacgg tacggttctt gttcgccccg tcgtagttgc
      661 aggtgatggt gccagcgcca atgttgacgt tggcgccgac ctcggagtcg ccgatgtagg
      721 cgagatggtt ggctttcgag cccttgccga cgcggctcgc cttgacctcg acgaagttgc
      781 cgatgtgcgc ctcgtcggca aggctcgcgc ccggccgcag ccgcgcgtac ggcccgacga
      841 tgcagcgcgc gccgacttcg gcttcgtcca gatgcgagaa cgcgcgcact tcggtgcctg
      901 cggcgatgct cacgttgcgc agcacgcagt tggggccgat gcgcacatga tcgccgagcg
      961 tcaccttccc ctcgaatacg cagttcacat cgatggatac gtcgcggccg cattcgagcg
     1021 agccgcgcac gtcgatgcgc gcggggtcgg cgagcgtcac cccgccctcg agcaggcgcg
     1081 ccgcctcgcg atgctgcacc acgcgctcca gcccggcgag atcctgcttg gagttgaccc
     1141 ctgcgacctc ccagcggtcc tcgaccttga ccgcgaagac gggtatgtcg tcggcgccgg
     1201 ccaggctcac gatgtcagtg aggtagtact cctgctgcgc gttctggttg gtgatctttg
     1261 cgagccaggc ggacaggtgc gaggcgcgca gcgcgaagaa gccggcgttg atctcgcgga
     1321 tcgccgcctc cgccgggttg gcgtccttct gctcgacgat gcgcgatacg cgccccgccg
     1381 aggcgcgcac gatgcgcccg taccccgtcg ggttggcgac ttcggcggtc agcaccgcga
     1441 cgccccgctg cgaagcgtcg agcagccgct tgagcgtttc ggcgcgcacc agcggcacgt
     1501 cgccgtacag gatcagcacc tcggcatcgg cggcgacctg cggcattgcc tgccgcacgg
     1561 cgtgcccggt gccgagctgc tcggtctgcg ccacccattc gatcgccgga tcgggaaacg
     1621 cggcgcgcac ctcatcgccg ccgtggccgt ggaccacgac gaccttgcgc ggcttgagcg
     1681 cgcgcgccgc gtcgagcacg tgcgcgagga gcggccggcc ggcgagcgcg tgcaggacct
     1741 tcggaagcgc ggaatgcatg cgcttgccct gacccgcggc aagcacgatc acgtcgagcg
     1801 gcatggcgcg attctaggct aatattttcc ctgaggaggc agccatgaac atcgtaaccg
     1861 cgtgcgccgg catcatgctg tcgctcggca ccgtccaggc gttcgcgcag gacagctgcg
     1921 ccaaccgcgg cgagctcgac gcgctttact gcgacgagaa ccgcgacctg gtcgccgaca
     1981 cgccgaagga tccgaagaag ctgaagaacc cttccacgct ggtcttcacc tacacgccgg
     2041 tcgaggatcc ggcggtctac gagaacgtct tcaagccgtt caccgactat ctgtcgaagt
     2101 gcaccgccaa gcgcgtggtg ttctaccagg tgcagtcgaa cgccgccgag atcgaggcga
     2161 tgcgctccgg ccggctgcac gtcggcggct tctccaccgg cccgaccaac tacgcggtga
     2221 accttgccgg cgccgtcccc tttgcagtaa aggggacggc taccgaactg cagggctacc
     2281 agctgatctt cgttgtcagg aaggacagcc cctaccagaa gctcgccgac ctcaagggca
     2341 agaaagtcgc gcacgtgcag ccctcgtcca actccggcaa cctcgcgccg cgcgtgctgt
     2401 ttcccaagga agggctggtg cccgacaagg actacaaggt gctctattcg ggcaagcacg
     2461 accagaccgt gatgggcgtg ctgtcgggcg acttcgacgg cggcccggtc gcctcggacg
     2521 tgttcaggcg gatgggtgag cgcggccaga tcaagaccga cgacttccgc atcatctacc
     2581 gcagccagac cttccccacc tcgtcgttcg cctacgcgca cgacctcgag cccaagctgc
     2641 gcgacacgat gctcaagtgc ttctacgact accgcttcac gcccgagatg cagaaggcct
     2701 tcgacggcgc cgaccgcttc tatccgatca cctacaagga gcactgggca gtggtgcggc
     2761 acgtggccga gggcagcggc gagaagttca acaaggcggc gttcgacaac ctgcagaagg
     2821 ccgaagccga ggccgccgcc aagaaggcgc agaagaaata gccctcgctg taaaagcgct
     2881 ggtcaaggag tacctgcccg gcaggccggt gctgcgcggc atcgacctcg agttcggcgc
     2941 gcaaggcatg accgcggtga tcggaccctc cggcaccggc aagtcgaccc tgctgcgttg
     3001 catcaaccgt ctcgtcgagc cgacctccgg cgaagtgatc tttgaggatc gggacgtaac
     3061 caggctttcg agaggcgagc tgcgcctggt ccgccggcgc atcggcatgg tgttccagga
     3121 gtacaacctg gtcgagcgcc tgacggtgat ggagaacctg ctgtgcgggc gcctgggcta
     3181 tgtctcgccc ttcaaggcct ggctgcggcg ctacccgccg cgcgacatcg cccgcgcctt
     3241 cgagctgctc gagaccgtcg gtctcgcggc gcatgcgc
//
LOCUS       DASYJA010000495        11302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2980212, whole genome shotgun sequence.
ACCESSION   DASYJA010000495 DASYJA010000000
VERSION     DASYJA010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11302
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2980212"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /gene="tgt"
                     /locus_tag="VGO02_10755"
     CDS             complement(<1..734)
                     /gene="tgt"
                     /locus_tag="VGO02_10755"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEV7478203.1"
                     /translation="MNFKIKNTDGKARRGSLELAHGRVETPAFMPVGTYGAVKAMSPA
                     ELRELGAQIVLGNTFHLWLRPGLDVIRAHGGLHRFMAWDGPILTDSGGFQVFSLGDLR
                     KISEEGVQFASPLNGDRLFLTPEISMDIQRTLDSDIAMVFDECTPYPATRDEAAASMQ
                     LSMRWAERCRKNWNSKNALFGIVQGGMYEELREESLEKLKAIGFEGYAVGGLSVGEPK
                     EDRSRILAHMGERLPAQSPRYLMGMGT"
     gene            complement(731..1717)
                     /gene="queA"
                     /locus_tag="VGO02_10760"
     CDS             complement(731..1717)
                     /gene="queA"
                     /locus_tag="VGO02_10760"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEV7478204.1"
                     /translation="MLVSEFDYALPPGLIAQHPAPRRRASRLLHLDAAGGLRDLQFAD
                     LPGLVGPGDALVLNDTRVIKARLAGRKASGGAVEIFVERLLGPREALALIRASHPPRP
                     GSDLFVEDIALKIVARHGDLYQVSFSQDVETVLERHGAVPLPPYITHAPQAEDAERYQ
                     TVYAAHPGAVAAPTAGLHFDDSMLSGIAAKGTLIEKITLHVGAGTFQPVRVDEVEKHR
                     MHKERYAIPPDAFERLSRKKVLAVGTTSLRALETAAATGKLEGETDLFVYPGFEFKVV
                     KRLLTNFHLPRSTLLMLACAFGGKERVLSAYHHAVQSKYRFFSYGDAMLIER"
     gene            1751..1837
                     /locus_tag="VGO02_10765"
     tRNA            1751..1837
                     /locus_tag="VGO02_10765"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1785..1787,aa:Leu,seq:caa)
     gene            1950..3047
                     /locus_tag="VGO02_10770"
     CDS             1950..3047
                     /locus_tag="VGO02_10770"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEV7478205.1"
                     /translation="MAQAQVRVLPSARNTVMVVDDQSTGRAILEQVVRSLDERVVVES
                     FARPIDAVVWATRHVSDLVLADYMMPDMDGIEFAKRLRALPGYEHVPIVMVTVHDDRK
                     VRYAALDAGITDFLSKPVDARECLARCRNLLTLRRQQLALEDRRRLLEHMVEDATREV
                     REREKETLLRLARAGEFRDEETGFHLIRMSRYSRIIASAIGLERDEVETVELAAPLHD
                     IGKIGIPDGILLKPEKLNAEEWDVMRRHPVIGHEILKGSASKYVRMGALIALGHHEKY
                     DGSGYPNGLVGDHIPLCARIVAVADVYDALTTARPYKKAWKREQANEYLEAQRGKHLD
                     PRLVDAFGAMHKEVMEVQNEWREPAAAEGKA"
     gene            3047..5590
                     /locus_tag="VGO02_10775"
     CDS             3047..5590
                     /locus_tag="VGO02_10775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7478206.1"
                     /translation="MEQPAAAKAPPRWLGGLRARLAARPDTEHEQGILRLVISSLVFL
                     YLLPDALTQRDLWPVYVMGIHLMLAVFIFLRIAYSTVVSPVRRVGAQFADVAAITWYM
                     TVFGESAAPLFLLYIWVTLGSGFRFGPKYLISELAMSVVGFGVALYVNPWWREHPALG
                     IGMLLGMLAVSMYVLSLVRRMFDALARAEAANLAKRRFISMVSHELRTPLNAIIGMAD
                     LLRDTALNREQADMLQTLRGSSRVMLGLVEDVLDFSKIEAGKVVLEKTEFDLHALVNS
                     TCRIVSAQAAAKGVEFVVSIMPEVPPAVRGDPHHLRQVLINLAGNAVKFTEVGSVTVH
                     ISVQAETDAIVRLKFSIRDTGIGISTEAQAKIFDSFAQADESTTRRFGGTGLGTTIAK
                     QLVGLMGGRIGVESAIGLGSTFWVEIDLEKQPERAEVGSGEIAGARIMLVGFPQPQRA
                     ALEEALAGWGATPIGVATVEEGVARLVSEISLAKPYHSALLYASGEDLTLAQRFRRAA
                     PDPAPATVLAVPRDADVRRFEVLASGFASVLELPFDKRRLFNVLHSVSAGDEVREGVV
                     RLQDYARRQTSSAKLRIVVADDNPTNREVVGKILERSGHTVTVVNDGEQALDAIERTA
                     PSLVILDRNMPGMGGMEALQALRLMMRDAERLPVIMLSADATPEAKREALEAGADAFL
                     PKPIEALRLLDEIQRLAGGKPEAAARPAQPAEEPTVLRRPGASPAAAVVNAETLAHLE
                     ELGSSLTFVEKLIRVFLSDNSTLLERIQQVLAGRNYHEFRSLVHAMKGSSASMGTDRL
                     TRLCSSLAKLSDAELRLQAPGLQRTLRDELEAARLEFESYLQQRRKSTG"
     gene            5605..6516
                     /locus_tag="VGO02_10780"
     CDS             5605..6516
                     /locus_tag="VGO02_10780"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7478207.1"
                     /translation="MDRSPFSARSELTPLRLRAADGYELGASLFAAAGAPEPKRVAVV
                     HCGAGIPAAHYRRFASFLAESGIPTLTYDYRGIGESRPRSLRGFRAGTEDWAEYDCGA
                     AIAWLRRRYPGAEMLGIAHSIAALIVGGAPNSAEQGRLCMICGHTGYFGDYHPRYRML
                     MTALWHGFMPAVTLLAGYFPARRFGLGEDLPARMALQWAGRRTPDLRPAGSGPGFERV
                     QAMLDRCASLQRPAVLVTIGDDAFATPGGTKRLLYYYPRLFPLQRIEFSPADAGLRRI
                     GHFGFFRRAAGAALWPRLLALLATKPG"
     gene            complement(6528..7466)
                     /locus_tag="VGO02_10785"
     CDS             complement(6528..7466)
                     /locus_tag="VGO02_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEV7478208.1"
                     /translation="MSNMHDAVGLCIPYGRAESQYETEFEPATGTLWGYFNPQDGIPC
                     YSLKLLKDILEHDQKLVANGCKVEIEGGMLPVNHYVTASRVRGVFNVGGDLALFAMLI
                     KARDRQALAHYARLCVDNMYPRTRGFFSPTLTKICLVQGDALGGGFECALASDVIVAE
                     ESAQMGLPEILFNLFPGMGACSLLARRIGIRAAEELILSGKIMSAVELHKMGVVDVLA
                     TDGQGETAVRSWIARNAKRRNGMQAVFRARQFIHPVTREELDGIVDLWIDAALRLTER
                     DLKMMNRLVRAQVRRMEAGCATPMEAAPLAEPLAAA"
     gene            7649..7885
                     /locus_tag="VGO02_10790"
     CDS             7649..7885
                     /locus_tag="VGO02_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478209.1"
                     /translation="MYAIVYKSDGFPICRQVAGVTPDPVVTWNTADSAKAFISSKGGD
                     AEFQPVEITDDSMDRLAKAMGCAVDVMTFEPYPG"
     gene            complement(7882..8688)
                     /gene="panC"
                     /locus_tag="VGO02_10795"
     CDS             complement(7882..8688)
                     /gene="panC"
                     /locus_tag="VGO02_10795"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEV7478210.1"
                     /translation="MKVVRKIGELRAALAAAAEAGPLAFVPTMGNLHAGHLSLARIAR
                     EHAKQVAVSIFVNRLQFRPGEDFDSYPRTFERDCAMLEAEGVDVLFAPDEAVLYPQKQ
                     DVIVHPGPLGDELEGRFRPGFFQGVATVVLKLLNSVQPVAAVFGKKDYQQLMVVRAMA
                     RQLDLPVRIVAGETVREADGLALSSRNSYLSAAERAEAPRLHRVLQMVKQGMHPDEAI
                     RVLTTAGWQPDYVEVRRRQDLAVPGADDRERVVLAAARLGGTRLIDNLEF"
     gene            complement(8688..9320)
                     /locus_tag="VGO02_10800"
     CDS             complement(8688..9320)
                     /locus_tag="VGO02_10800"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEV7478211.1"
                     /translation="MDKFRYLVVEGPIGAGKTSLARRLGARLSADLLLEQPAENPFLA
                     RFYDDMPRYALQVQLFFLFQRLRMLEPLAQPDMFGRPTVADFLLDKDPLFARITLSAD
                     ELALYEKIYQALRPRAPAPDLVIYLQAQPATLIERVRRRGAGYERGISEEYLALLTEG
                     YARFFYHYTLSPVLIVNSENLNFVERDADFELLVSRMRGMKSRREFFNLG"
     gene            complement(9313..9660)
                     /locus_tag="VGO02_10805"
     CDS             complement(9313..9660)
                     /locus_tag="VGO02_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HEV7478212.1"
                     /translation="MQLSTVATSAGLLVASNVFMTFAWYAHLKDLSSRPWWIAALVSW
                     GIALFEYLLQVPANRIGFTEMSLPQLKIMQEAITLVVFVPFAILYMKQPIKLDYLWAS
                     LCIMGAVYFVFRG"
     gene            complement(9660..10139)
                     /gene="folK"
                     /locus_tag="VGO02_10810"
     CDS             complement(9660..10139)
                     /gene="folK"
                     /locus_tag="VGO02_10810"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEV7478213.1"
                     /translation="MTLAYVGIGSNLDEPKSHVCLAFEDLAQIPGTTVVKRSSLYRSA
                     PVGYADQADFVNAVAEIETSLPATRLLAELKEIEARHGRHRSFRNAPRPLDLDILLYG
                     ELTMSLAHLTLPHPRMHERAFVLKPLMELSPEIIIPGVGSVKDCLEKTGDQIAERIE"
     gene            complement(10136..>11302)
                     /gene="pcnB"
                     /locus_tag="VGO02_10815"
     CDS             complement(10136..>11302)
                     /gene="pcnB"
                     /locus_tag="VGO02_10815"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEV7478214.1"
                     /translation="GAVRDLLLGIVPKDFDIATDARPEQVKPLFRRAIIIGRRFRLVH
                     VMMSQDTVEVSTFRAADPDTSEKDQHGRVLRDNVFGTQQEDARRRDFTVNALYFDPAT
                     EEVVDFHGGLADLKKRVMRVIGDPETRYREDPVRMLRAVRLGAKLGLTLDAATREPIR
                     ELAPLMEQVPPARLFDEMLKLLLSGHASACLRQLREVGLSKGLLPLLDVILEQPLGER
                     FVTLALAQTDERVLAGRPVSPAFLFAALLWHEVLAASKARQARGERPVPALEAAMDEV
                     LDTQCAKLAITRKLTATMREVWAMQPRFENRSGNRAYRLLEAPRFRMAYDFLALRAAS
                     GEVPAELEAWWRAFQSADDETRKAMLLPDTGPKKRRRRRRRGGGGAPAQGPEPA"
BASE COUNT         1743 a         3855 c         3883 g         1821 t
ORIGIN      
        1 gtgcccatgc ccatcaggta gcgggggctt tgcgccggca gcctctcgcc catgtgcgcc
       61 aggatgcgcg agcggtcttc tttcggctcg cccaccgaca gcccgcccac cgcgtagccc
      121 tcgaacccga tggctttcag cttctcgagg gactcctctc tcagctcttc gtacatgcct
      181 ccctgcacga tcccaaaaag cgcgttttta ctgttccagt tttttctgca tctttccgcc
      241 cagcgcatcg ataactgcat cgacgcagct gcctcgtccc gggtcgccgg gtaaggggtg
      301 cactcgtcga acaccatcgc gatgtccgag tcgagcgtgc gctggatgtc catcgagatc
      361 tcgggagtca ggaacaggcg gtcgccgttg agcggcgagg cgaactgcac gccttcctcg
      421 ctgatctttc tcaggtcccc caggctgaac acctggaaac cgcccgagtc ggtgaggatc
      481 ggcccgtccc aggccatgaa gcggtgcagg ccgccgtgcg cccggatgac gtccaggccc
      541 gggcgcagcc acaggtggaa ggtgttgccg agcacgatct gcgcgccgag ctcgcgcagc
      601 tccgcgggcg acatcgcttt taccgcgccg taggtgccca ccggcatgaa cgccggcgtc
      661 tccacgcggc cgtgcgcgag ctcgaggctg ccgcgccttg cctttccatc cgtgtttttt
      721 attttgaaat tcatctttcg atgagcatcg catcgccgta agagaagaag cggtacttgc
      781 tctgcacggc gtgatgatag gcgctcagca cgcgctcctt gccgccgaag gcgcacgcga
      841 gcatcagcag ggtcgacctg ggcaggtgga aattggtgag aaggcgcttc accaccttga
      901 actcgaagcc cgggtagacg aacagatcgg tctcgccttc gagctttcct gtcgccgctg
      961 ccgtctcgag cgctcgcagc gacgtggtcc ccacggctag gacttttttt cgtgaaagcc
     1021 gctcgaaagc gtccggaggt atcgcgtagc gctccttgtg catgcgatgc ttctcgactt
     1081 catcgacccg cacgggctgg aaagtcccgg cgccgacgtg cagcgtgatc ttttcgatca
     1141 gcgtcccctt tgcggcaatg ccggaaagca tcgaatcgtc gaagtgcagg ccggcggtgg
     1201 gcgcggcgac ggcgcccggg tgagcggcgt agacggtctg gtagcgctcg gcgtcctctg
     1261 cctgcggcgc atgggtgatg taaggcggca gcgggaccgc gccgtgacgc tccaggactg
     1321 tttccacatc ttgtgaaaat gaaacctggt agaggtcgcc gtgccgcgca acgattttca
     1381 aggcaatgtc ttcgacgaaa aggtcgctgc cggggcgcgg cgggtggctg gcgcggatca
     1441 gcgccagggc ctcgcgcggg ccgagcaggc gctcgacgaa gatctcgacg gcgccgccgc
     1501 tggccttgcg gccggcgagg cgcgccttga tgacgcgggt gtcgttgagg accagcgcgt
     1561 cgccgggacc caccaggccg ggcaaatcgg cgaactgcag gtcgcgcagc ccgccggcgg
     1621 cgtccaggtg cagcagccgg ctcgcgcggc ggcgcggcgc cgggtgctgc gcgatcagcc
     1681 cgggcggcag ggcatagtcg aactcggaga caagcatggg gggcgcatta taatttcgcc
     1741 ccctttccga gccggaatgg tggaactggt agacgcagcg gactcaaaat ccgccgaccg
     1801 caaggtcatg agagttcgag tctctcttcc ggcaccagcc gagcgccccg gaagcaggta
     1861 aaagatcgtc caaaaccggg ttttccccgc tggactgggc gcgcgcccgg gcgtaggctc
     1921 cccaagagaa agtcagaggg gggacgtcca tggcgcaggc gcaagtcagg gtactgcctt
     1981 cggcgcgcaa taccgtgatg gtggtcgacg accagtcgac cggccgcgcc attctcgagc
     2041 aggtggtgag gagcctcgac gagcgcgtgg tggtggagag cttcgcgcgc ccgatcgacg
     2101 ccgtggtctg ggcgacgcgc cacgtctccg acctggtgct cgccgactac atgatgccgg
     2161 acatggacgg catcgagttc gccaagcgcc tgcgcgcgct gccgggctac gagcacgtgc
     2221 cgatcgtgat ggtcacggtg cacgacgacc gcaaggtgcg ctacgccgcg ctcgacgccg
     2281 gcatcaccga tttcctgtcc aagccggtcg acgcgcgcga gtgcctggcg cgctgccgca
     2341 acctgctcac gctgcgccgc cagcagctcg cgctggagga ccgccggcgc ctgctcgagc
     2401 acatggtgga ggacgcgacg cgcgaggtgc gcgagcgcga gaaggagacg ctgctgcgcc
     2461 tcgcccgcgc cggcgagttc cgcgacgagg agaccggctt tcacctgatc cgcatgtcgc
     2521 gctactcgcg gatcatcgcc agcgccatcg gcctggagcg cgacgaggtg gagacggtgg
     2581 agctcgccgc gccgctgcac gacatcggca agatcggcat ccccgacggc atcctgctca
     2641 agccggagaa gctcaacgcc gaagagtggg acgtgatgcg ccgccacccg gtgatcggcc
     2701 atgagattct caagggcagc gcctcgaagt acgtgcgcat gggcgcgctg atcgccctcg
     2761 gccaccacga gaagtacgac ggctcgggct acccgaacgg gctggtcggc gaccacatcc
     2821 cgctgtgcgc gcgcatcgtc gccgtcgccg acgtgtacga cgcgctcacc accgcccggc
     2881 cgtacaagaa ggcgtggaag cgcgagcagg ccaacgagta cctcgaggcg cagcgcggca
     2941 agcacctcga cccgcgcctg gtggacgcct tcggcgcgat gcacaaggaa gtgatggaag
     3001 tgcagaacga gtggcgcgag ccggccgcgg ccgagggcaa ggcgtagtgg agcagccggc
     3061 cgccgccaag gcgccgccgc gctggctggg cgggctgcgc gcgcgccttg ccgcgcggcc
     3121 cgacaccgag cacgagcagg gcatcctgcg gctggtgatc agctcgctgg tcttcctcta
     3181 tctcctgccc gacgcgctca cgcagcgcga cttgtggccg gtgtacgtga tgggcatcca
     3241 cctgatgctc gcagtgttca tcttcctgcg catcgcctac tcgacggtgg tgtcgccggt
     3301 gcgccgtgtc ggcgcgcagt tcgccgacgt agcggcgatc acctggtaca tgacggtgtt
     3361 cggcgagtcg gcggcgcccc tcttcctgct ctacatctgg gtcacgctcg gcagcggctt
     3421 ccgcttcggc ccgaagtacc tgatctccga gctggcgatg agcgtggtcg gcttcggcgt
     3481 cgcgctctac gtcaatccct ggtggcgcga gcacccggcg ctcggcatcg gcatgctgct
     3541 cggcatgctc gcggtgagca tgtacgtgct gtcgctggtg cggcgcatgt tcgacgcgct
     3601 ggcgcgcgcc gaggcggcca acctggccaa gcgccgcttc atctcgatgg tcagccacga
     3661 gctgcgcacg ccgctcaacg ccatcatcgg catggccgac ctgctgcgcg acaccgcgct
     3721 caaccgcgag caggccgaca tgctgcagac gctgcgcggc tcctcgcgcg tcatgctcgg
     3781 cctggtcgag gacgtgctcg acttctccaa gatcgaggcc ggcaaggtgg tgctggagaa
     3841 gaccgagttc gacctgcacg cgctggtcaa cagcacctgc cgcatcgtct cggcgcaggc
     3901 tgcggcgaag ggcgtcgagt tcgtcgtgtc gatcatgccc gaggtgccgc ccgcggtgcg
     3961 cggcgacccg caccacctgc gtcaggtgct gatcaacctg gcgggcaacg cggtcaagtt
     4021 caccgaggtg ggcagcgtca cggtgcacat ctcggtgcag gccgagaccg acgccatcgt
     4081 gcgcctgaag ttctccatcc gcgacactgg catcggcatc tcgaccgagg cgcaggccaa
     4141 gatcttcgac agcttcgcgc aggcggacga atcgaccacg cgccgcttcg gcggcaccgg
     4201 cctgggcacc accatcgcca agcagctcgt cggcctgatg ggcgggcgca tcggcgtgga
     4261 gagcgcgatc gggctgggca gcaccttctg ggtcgagatc gacctggaga agcagcccga
     4321 gcgcgccgag gtcggcagcg gcgaaatcgc cggcgcacgc atcatgctcg tcggcttccc
     4381 gcagccgcag cgcgcggcgc tcgaggaggc gctggccggc tggggtgcga cgccgatcgg
     4441 cgtcgccacg gtagaagaag gcgtggcgcg cctggtttcg gaaatcagcc tggcaaagcc
     4501 ctaccacagc gcgctgctct acgcgagcgg cgaggacctg acgctcgcgc agcgcttccg
     4561 ccgcgccgcg cccgacccgg cgccggctac cgtgctcgcc gtgccgcgcg acgccgacgt
     4621 caggcgcttc gaggtgcttg cctccggctt cgcctcggtg ctcgagctgc ccttcgacaa
     4681 gcggcgcttg ttcaacgtgc tgcattcggt ctcggcgggg gacgaggtgc gcgaaggggt
     4741 ggtgcgcctg caggactacg ctcggcgcca gacgagctcc gccaagctgc gcatcgtcgt
     4801 ggccgacgac aacccgacca accgcgaagt ggtcggcaag atcctcgagc gcagcggcca
     4861 caccgtgacc gtggtgaacg acggcgagca ggcgctcgac gcgatcgagc gtaccgcgcc
     4921 cagcctggtg atcctcgacc gcaacatgcc cggcatgggc ggcatggaag cgctccaggc
     4981 gctgcgcctg atgatgcgcg acgccgagcg cctgccggtc atcatgctgt cggccgacgc
     5041 cacgcccgag gcgaagcgcg aggcgctcga ggcgggggcc gacgccttcc tgcccaagcc
     5101 gatcgaggcc ttgcggctgc tcgacgaaat ccagcggctg gcgggcggca agcccgaggc
     5161 tgcggcgcgc cccgcccagc cggccgagga gccgactgtg ctgcgccgcc ccggcgcctc
     5221 gcccgcggcc gccgtggtga atgccgagac gctggcgcac ctcgaggagc tcggctcgtc
     5281 gctcactttc gtcgagaagc tgatccgcgt cttcctcagc gacaacagca cgctcctcga
     5341 gcgcatccag caggtgctcg ccggccgcaa ctaccacgag ttccgctcgc tggtgcatgc
     5401 gatgaagggc tcgtcggcga gcatgggcac cgaccgcctg acgcgcctgt gcagcagcct
     5461 cgccaagctg tcggatgccg agctgcggct gcaggcaccc gggctgcagc gcacgttgcg
     5521 cgacgagctg gaggcggcgc gcctcgagtt cgagagctac ctgcagcagc ggcgcaaatc
     5581 caccggttag cttatcgttg gtaggtggac cgctccccct tctcggcacg tagcgagttg
     5641 acgccgctgc gcttgcgcgc agctgacggc tacgagctcg gcgcctcgct ctttgccgcc
     5701 gccggtgcgc cagagccgaa gcgcgtggcg gttgtgcact gcggcgcagg tatcccggcg
     5761 gctcactatc gccgcttcgc cagctttctc gccgaatcgg gcattcccac gctgacctac
     5821 gactaccgtg gtatcggcga gtcgcgtccg cgctcgctgc gcgggttccg ggccggcacg
     5881 gaggactggg cggaatacga ttgcggcgcg gccatcgctt ggctgcgccg gcgctaccct
     5941 ggcgccgaga tgctgggcat cgcgcactcg attgccgcgc tgatcgtcgg cggggcgccc
     6001 aactcggccg agcagggccg cctctgcatg atctgcgggc ataccggcta cttcggtgat
     6061 taccacccgc gctaccgcat gctcatgacg gcgctctggc acggcttcat gcctgcggtc
     6121 accctcctgg ccgggtactt tccggctcgc cgtttcgggc tcggcgagga cctcccggcc
     6181 aggatggcgc tgcagtgggc cggccgtcgc acgccggacc tgcggccagc cgggtcgggg
     6241 cccggcttcg agagggtgca ggcgatgctc gatcgttgcg ccagcctgca gcggccggcg
     6301 gtcctagtca cgatcggcga cgacgcgttc gccactcccg gcggtaccaa gcgcctcctg
     6361 tactactacc cgcgcctgtt cccgctgcag cgcatcgaat tcagcccggc cgacgcgggg
     6421 ctgcgccgca tcggccattt cggcttcttc cgccgcgccg ccggtgcggc cctctggccg
     6481 cgcctgctgg cgctgctcgc aacgaagccg ggctagctag cgccggctca ggccgccgcc
     6541 aggggctcgg cgagcggcgc cgcttccatc ggcgtcgcgc agccggcctc catgcggcgc
     6601 acctgggcgc gcaccaggcg gttcatcatc ttcaggtcgc gctcggtgag gcgcagcgcc
     6661 gcgtcgatcc acaggtcgac gatgccgtcg agctcctcgc gcgtcacggg atggatgaac
     6721 tggcgggcgc ggaacacggc ctgcatgccg ttgcgccgct tggcgttgcg cgcgatccag
     6781 ctgcgcaccg cggtctcgcc ctggccgtcg gtggccagca cgtcgaccac gcccatcttg
     6841 tgcagctcga ccgcgctcat gatcttgccg gagaggatca gctcctcggc ggcgcggatg
     6901 ccgatgcgcc gcgcgagcag gctgcaggcg cccattcccg ggaacaggtt gaacaggatc
     6961 tcgggcaggc ccatctgcgc gctttcctcg gccacgatga cgtccgaggc gagggcgcac
     7021 tcgaagccgc cgcccagcgc gtcgccctgc accaggcaga tcttggtgag ggtcgggctg
     7081 aagaagccgc gcgtgcgcgg gtacatgttg tcgacgcaca gcctggcgta gtgggccagc
     7141 gcctgccggt cgcgcgcctt gatcagcatg gcgaacagcg ccaggtcgcc gccgacgttg
     7201 aacaccccgc gcacgcgcga ggcggtcacg tagtggttca ccggcagcat cccgccttcg
     7261 atctcgacct tgcagccgtt ggccaccagc ttctggtcgt gctccaggat gtccttcagc
     7321 agcttgaggc tgtagcaagg gatgccgtcc tgggggttga agtagcccca gagcgtgccg
     7381 gtggccggct cgaactccgt ctcatactgc gattcggcgc ggccgtaggg gatgcaaagc
     7441 cccactgcgt cgtgcatgtt gctcattgcc ttctccttcc ccgaaagtgc ccccctaggc
     7501 gggggctggt gtcccagctc cgttgcgccg gagcctcgcg gccatttaat gccacgtgtg
     7561 taaaaagtcg caatcgggga gtcccctagg gaagccgcct tgttgccgca gtgccacatc
     7621 gggtaaagta ggacggcggg gggagggcat gtacgcgatc gtttacaagt cggacgggtt
     7681 tccgatctgc cggcaggtcg cgggggtgac ccccgacccg gtggtgacct ggaacaccgc
     7741 ggactccgcc aaggccttca tctcctccaa gggcggcgac gccgaattcc agcccgtgga
     7801 gatcaccgac gactcgatgg acaggctagc aaaagcaatg ggctgcgcgg tcgacgtgat
     7861 gaccttcgag ccctatcccg gctagaactc caggttatcg atcagtcttg tcccgcccag
     7921 ccgggcggcc gccagaacca cccgttccct gtcgtctgcc cctggtacag ccaggtcctg
     7981 ccgccgtctc acttcgacgt agtcgggttg ccatccggct gtcgtcagta ccctgattgc
     8041 ctcatcaggg tgcatcccct gtttcaccat ctggaggacc cggtgcagcc tgggggcctc
     8101 ggcccgctcg gccgccgaga ggtagctgtt gcgtgagctc agggcgaggc cgtcggcctc
     8161 gcgcactgtc tcgccggcga cgatcctgac cggcaggtcg agctggcgcg ccatcgcgcg
     8221 caccaccatc agctgctggt agtccttctt gccgaacacc gccgccaccg gctgcacgct
     8281 gttcagcagc ttgagcacca ccgtcgccac gccctggaag aagccggggc gaaagcggcc
     8341 ctccagctca tcgcccagcg gccccgggtg cacgatgacg tcctgcttct gcgggtagag
     8401 gaccgcttcg tcgggcgcga acagcacgtc caccccttcg gcctccagca tggcgcagtc
     8461 gcgctcgaag gtgcgcgggt agctgtcgaa atcctcgccc ggacggaact gcagccggtt
     8521 gacaaagatg ctcaccgcca cctgcttcgc atgctcgcgc gcgatccgcg ccagtgacag
     8581 gtggcccgcg tgcagattgc ccatcgtcgg cacgaaggcg agcgggcccg cctccgccgc
     8641 cgcggcgagg gccgcgcgca gttctcctat cttgcgcacg actttcatca gccgaggttg
     8701 aagaactcgc gccggctctt catgccgcgc atgcgcgaca ccagcagctc gaagtcggcg
     8761 tcgcgctcga cgaagttgag gttctcggag ttcacgatca gcaccggcga gagcgtgtag
     8821 tggtagaaga agcgcgcgta gccctcggtg agcagcgcca ggtactcctc gctgatgccg
     8881 cgctcgtagc ccgcgccgcg ccggcgcacg cgctcgatca gcgtcgccgg ctgcgcctgc
     8941 aggtagatca ccaggtcggg cgccggcgcg cgcggccgca gcgcctggta gatcttctcg
     9001 tagagcgcga gctcgtcggc cgacagcgtg atgcgcgcga agagcgggtc cttgtcgagc
     9061 aggaagtcgg ccaccgtcgg ccggccgaac atgtcgggct gcgccagcgg ctcgagcatg
     9121 cgcaggcgct ggaacaggaa gaagagctgc acctggagcg cgtagcgcgg catgtcgtcg
     9181 tagaagcgcg cgaggaacgg gttctcggcc ggctgctcga gcagtagatc cgccgaaagg
     9241 cgcgcgccga ggcgtcgcgc gaggctggtc ttgccggccc cgatggggcc ttccaccacc
     9301 agataacgga atttatccac ggaaaacgaa gtacacggct cccatgatgc acagcgacgc
     9361 ccacaggtag tcgagcttga tgggctgctt catgtagagg atggcgaagg gcacgaacac
     9421 caccagcgtg atcgcctcct gcatgatctt gagctgcggc agcgacatct cggtgaaccc
     9481 tatccggttg gccggcacct gcagcaggta ctcgaaaaga gcgatgcccc agctcaccag
     9541 cgccgcgatc caccacggcc tggacgagag gtccttcagg tgggcgtacc aggcgaaggt
     9601 catgaatacg ttcgaggcga cgagcagccc ggcggaggtg gctacggtag agagctgcat
     9661 cactcgatcc tttccgctat ctggtcgccg gtcttttcaa ggcagtcctt caccgacccc
     9721 acgcccggaa taatgatttc aggagaaagt tccattaagg gcttcaggac gaacgcccgc
     9781 tcgtgcatgc gcggatgcgg cagcgtcagg tgcgcgaggc tcatcgtgag ctctccgtag
     9841 aggaggatgt ccaggtcgag ggggcggggg gcgttgcgga agctgcggtg ccggccgtgc
     9901 cgtgcctcga tctccttcag ctcggcgagt aggcgggtgg ccggcaggct ggtctcgatc
     9961 tcggccacgg cgttgacgaa atcggcctgg tccgcatagc cgaccggcgc ggagcggtac
    10021 agggaagagc gcttcactac ggtagtgccc gggatttgag cgaggtcctc gaaagcgagg
    10081 cagacatggc ttttcggctc gtccaggttc gacccgatgc cgacgtaagc cagcgtcacg
    10141 cgggctcggg gccttgcgct ggcgcgccgc cgcctccgcg gcggcgccgg cgacgccgtt
    10201 ttttcgggcc ggtgtcgggc agcagcatgg ccttgcgcgt ctcgtcgtcg gcgctctgga
    10261 aggcgcgcca ccacgcctcg agctcggcgg gcacctcgcc gctcgccgcg cgcagcgcga
    10321 ggaagtcgta ggccatgcga aagcgcggcg cctcgagcag gcggtaggcg cggttgcccg
    10381 agcggttctc gaagcgcggc tgcatcgccc acacctcgcg catggtcgcg gtgagcttgc
    10441 gcgtgatggc gagcttggcg cactgcgtgt cgagcacctc gtccatcgcc gcctcgagcg
    10501 cgggcaccgg ccgctcgccg cgcgcctggc gcgctttcga cgcggcgagc acctcgtgcc
    10561 agaggagcgc ggcgaagagg aaggcgggcg acaccggccg cccggcgagc acgcgctcgt
    10621 cggtctgcgc gagcgcgagg gtgacgaagc gctcgcccag gggctgctcg aggatcacgt
    10681 cgagcagcgg cagcaggccc ttgctcaggc cgacctcgcg cagctggcgc aggcaggcgc
    10741 tggcgtggcc cgagagcagc agcttcagca tctcgtcgaa caggcgcgcc ggcggcacct
    10801 gctccatgag cggcgccagc tcgcggatcg gctcgcgggt ggcggcgtcg agggtcaatc
    10861 caagcttggc tcccaatctg accgcgcgca gcatgcgtac cggatcttcc cgatagcgtg
    10921 tttctggatc cccgatgacc cgcatcacgc gcttcttcag gtcggccagg ccgccgtgga
    10981 aatcgaccac ctcctcggtg gccgggtcga agtacagcgc gttgacggtg aagtcgcgcc
    11041 ggcgcgcgtc ctcctgctgg gtgccgaaga cgttgtcgcg cagcacgcgg ccgtgctggt
    11101 ctttctcgga agtgtcgggg tcggcagcgc ggaaggtcga gacctccacc gtgtcctggc
    11161 tcatcatcac gtgcaccagg cggaagcgcc ggccgatgat gatcgcgcgg cggaacaggg
    11221 gcttcacctg ctcggggcgc gcgtcggtgg cgatgtcgaa gtccttgggc acgatgccca
    11281 gcagcaggtc gcgcaccgcg cc
//
LOCUS       DASYJA010000496         1675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2708193, whole genome shotgun sequence.
ACCESSION   DASYJA010000496 DASYJA010000000
VERSION     DASYJA010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2708193"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VGO02_10820"
     CDS             complement(<1..606)
                     /locus_tag="VGO02_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEV7478215.1"
                     /translation="MNTISPTDCPAWAKLSHHAEGWRDVHLRDLFANDVARSLQFVAE
                     APGLRYDYSRQRVGAITLRLLSNLAAERGLPKWRAALLSGKPVNTSENRAAWHTALRA
                     GKASPAEVLQTLGSMQSIASRIRSEGKFKRIINLGTGGSDLGPRLLADALGDGALDVR
                     FAANVDPRDLARALEGADPATTLFIIVSKTFTTQETMANAMA"
     gene            complement(618..>1675)
                     /locus_tag="VGO02_10825"
     CDS             complement(618..>1675)
                     /locus_tag="VGO02_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478216.1"
                     /translation="LALGLTVAAAHLVCEEWRNRSGLLFSVSFVLIAALIAGAWPLAL
                     YLRSPELFTYWWLLASLPQGGFGSNLRYFLVTASWFTWPAWPIAAWALWSLRRRLAEP
                     RLFVPFAASAAMLAAIGFWGPQQDVNLIMLLPPLALLAAQGALVLRRGAAGALDWFGV
                     LAFGFFTFLVWLGYFSMLTGLPPRLANNFFKSAPGFVLELKAWPLLLAAALFLGWLYV
                     MFFSTRSPVRSVARWAAGIVLLWGSFATLWMPWADYVKSYRSVALQLRSKIPVDAGCI
                     AQRSLGISQAAALDYHAGIRPQPFDTLKPEACPLLLVQGSPKNEFDGPGPGWAKLADV
                     GRPGDRSERYRLYQFSK"
BASE COUNT          303 a          540 c          612 g          220 t
ORIGIN      
        1 cgccatggcg ttggccatgg tttcctgcgt ggtgaaggtc ttcgacacga ttatgaacag
       61 cgtggtggcc gggtcggcgc cctcgagcgc gcgcgcgagg tcgcgcgggt cgacgtttgc
      121 ggcgaaacgc acgtcgagcg cgccgtcgcc gagcgcatcg gcgagaaggc gcggtcccag
      181 atccgagccg ccggtgccga ggtttatgat tctcttgaat tttccttcgc ttcgaatcct
      241 cgaggcaata ctctgcatgc tgcccagcgt ctggaggacc tccgcaggag aagccttgcc
      301 tgctcgcagc gccgtatgcc acgccgcgcg gttttcgctc gtgttgaccg gcttgccgga
      361 gagcagcgct gcgcgccact tgggtaagcc gcgctcggcg gcgaggttgg agagcaggcg
      421 cagcgtgatg gcgcctacgc gctggcgcga gtagtcgtag cgcagccccg gtgcttcggc
      481 gacaaattgc aagctgcgcg cgacgtcgtt cgcgaagaga tccctgagat gcacgtcgcg
      541 ccagccctcc gcgtgatgcg agagcttggc ccatgccggg cagtcggttg gagaaatggt
      601 gttcaagagg aaagaatcta cttggaaaac tgataaagac gatagcgctc ggacctgtcg
      661 ccgggccggc cgacgtcggc gagcttggcc cagcccgggc cggggccgtc gaactcgttc
      721 ttcgggctgc cctgcaccag gagcagcggg caggcttcgg gcttcagggt gtcgaagggc
      781 tgcggccgga tgccggcgtg gtagtcgagc gcggccgcct gcgagatgcc gagggagcgc
      841 tgcgcgatgc agccggcgtc gaccgggatc ttcgagcgca gctgcagcgc caccgagcgg
      901 tagctcttca catagtccgc ccacggcatc cacagcgtgg cgaagctgcc ccacagcagc
      961 acgatgcccg cagcccagcg cgccacgctg cgcaccggcg agcgcgtcga gaagaacatc
     1021 acgtacagcc agccgaggaa cagcgcggcc gccaacagca gcggccaggc cttgagctcg
     1081 agcacgaagc ccggcgcgct cttgaagaaa ttgttggcga ggcgcggcgg caggccggtg
     1141 agcatggaaa agtagccgag ccagacgagg aaggtgaaaa agccgaaggc gagcacgccg
     1201 aaccagtcga gcgcgccggc ggcgccgcgc cgcaggacca gcgcgccctg cgcggcgagc
     1261 agcgcgaggg gcggcagcag catgatcagg ttgacgtcct gctgcggccc ccagaagccg
     1321 atcgcggcga gcatggccgc cgacgcggcg aaggggacga acaggcgcgg ctcggccagg
     1381 cgccggcgca gcgaccacag cgcccaggcc gcgatcggcc atgccggcca ggtgaaccag
     1441 ctcgccgtga cgaggaagta gcgcaggttg ctgccgaagc cgccctgcgg cagcgaggcg
     1501 agcagccacc agtaagtgaa cagctcgggc gagcgcaggt acagcgccag cggccaggcg
     1561 ccggcgatca gcgccgcgat gaggacgaaa gaaacgctga acagcaggcc gctgcggttg
     1621 cgccattcct cgcagaccag gtgcgccgcg gcgaccgtca ggcccagggc gagcg
//
LOCUS       DASYJA010000497         2887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2708387, whole genome shotgun sequence.
ACCESSION   DASYJA010000497 DASYJA010000000
VERSION     DASYJA010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2708387"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VGO02_10830"
     CDS             <1..411
                     /locus_tag="VGO02_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478217.1"
                     /translation="KLSHALVHKIPSLLEYKSLTSLDMPEVETILVEDEHPDPNLPFG
                     AKEVGQGPLLPVMPAVANAIYDAVGVRIDELPITPDKVLKALELKAAGKNPRIGPQSF
                     PSVPYPEPVVVLPPWEGGDGNAVKGAERPKVKVA"
     gene            411..1406
                     /locus_tag="VGO02_10835"
     CDS             411..1406
                     /locus_tag="VGO02_10835"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEV7478218.1"
                     /translation="MRLPWFEHRAPSTLAEAAHILAGEGPNAMLIAGGTDLVPNMKRR
                     QMAPKILVSTKKINELRKNGHALGAGTTLTEIVNRRDLPMGLRQAAHLVATVHLRNMG
                     TIGGNLCLDTRCNYYNQNYEWRKAIDFCLKKDGDICWVATASKRCVAASSTDCAPALM
                     AIGAKVRLVSSKDERSVALEDLYNNDGIDYLKRRPDEILTEISFEKDVKSTYWKLRRR
                     GAFDFPVLGVGAAVRMNGNVVETARIALGAVASRPFLVEKASLFLQNKKLTDEVIEEA
                     AKLVASRAKPMDNADLDLYWRKDVSVQFVEYALKELRGDDMRETRLKTARHVLDG"
     gene            complement(1403..2251)
                     /locus_tag="VGO02_10840"
     CDS             complement(1403..2251)
                     /locus_tag="VGO02_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEV7478219.1"
                     /translation="MDTITLAGPLEAKLRAVREAGFTQIMLNATDIVGHPGGEAAAVA
                     AVRASGLRVTGFQVLRDFEGLSGTLHAYKVDVAKAMLEMCHALGSKVLLACSTASPHS
                     SKDVSLIKKDLQKLAMLAVPLDIRIAYEALSWGRHVNEFPAAWEIVSEADRANLGLAL
                     DSFHILANRTDLELLQEVDPRKVFLVQLSDFLWQEVRSAEERIETARHSRVFPGEGVH
                     SDKVAALVRELDEMGYRGDYSFEVFNDDYRQLPLPMVAERARRSVKWITGLVSRRSLP
                     ARRKPA"
     gene            complement(2269..>2887)
                     /locus_tag="VGO02_10845"
     CDS             complement(2269..>2887)
                     /locus_tag="VGO02_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEV7478220.1"
                     /translation="AMAFRVRDAGQAFQHSLDLGAWEMPTRAAAMELHIPGIHGVGDS
                     LIYFIDRYQDFAIYDVDFVFDSNAKPKPPAVAGLHWFGVVQAILPERTADWLDFYRSL
                     FGFQVLPRGQYFGVLPKGTLLESPCHKFYLQLIEPPPGAEDVRWNEGLVRVGLGATDV
                     PKAVKALQERGVVFVDHGAVQPSDKGALTQIYLGGVTFELVVSSL"
BASE COUNT          556 a          922 c          945 g          464 t
ORIGIN      
        1 aagctgtcgc acgcgctggt gcacaagatt ccatcgctgc tcgaatacaa gagcctgacc
       61 tcactcgaca tgccggaagt cgagaccatc ctggtcgagg acgagcaccc ggatccgaac
      121 cttccgttcg gcgcgaagga agtcgggcag gggccgctgc tgccggtgat gccggcggtg
      181 gcgaacgcca tctacgacgc ggtcggcgtg cgcatagacg agctgccgat cacgccggac
      241 aaggtgctga aggctcttga attgaaggct gcaggcaaga atccgcgcat cggcccgcag
      301 agcttcccgt cggttcctta tccggaaccg gttgtcgtgt tgccgccttg ggagggtggt
      361 gacggcaatg ccgtgaaggg cgcagaacgt cccaaggtca aagtcgcatg atgcgcctgc
      421 cgtggttcga gcaccgcgcg ccctcgacgc ttgcggaagc cgcgcacatc ctggccggcg
      481 aaggcccgaa cgcgatgctc atcgccggcg gcaccgacct cgtgccgaac atgaagcgcc
      541 ggcaaatggc gccgaaaatc ctggtttcca cgaagaaaat aaatgagctg agaaaaaatg
      601 gccatgccct tggcgccggc acgaccctta cagaaatcgt aaaccgcaga gacctgccga
      661 tgggcctgcg ccaggccgcg cacctggtgg cgacggtgca cctgcgcaac atgggcacca
      721 tcggcggcaa cctgtgcctc gacacgcgct gcaactacta caaccagaac tacgagtggc
      781 gcaaggcgat cgacttctgc ctgaagaagg atggcgacat ctgctgggtc gccaccgcca
      841 gcaagcgctg cgtcgccgcc tcgtccaccg attgcgcgcc ggccctgatg gcgatcggcg
      901 cgaaagtaag gctggtttcg tcgaaggatg aacgaagcgt tgcgctcgag gacctctaca
      961 acaacgacgg catcgactac ctgaagcgcc ggcccgacga gatcctcacc gagatttcct
     1021 tcgagaagga cgtgaagagc acctattgga agttgcgaag gcgaggggct ttcgactttc
     1081 cggtgttggg tgtcggcgcc gccgtcagga tgaatggaaa cgtcgttgaa accgcgcgca
     1141 tcgcgctggg cgcagtggcc tccaggcctt tcctggtcga aaaggcttcg ttgtttttac
     1201 agaataagaa actaacggac gaagtgatcg aagaagcggc gaagctggtg gcctcgcggg
     1261 cgaagccgat ggacaacgcc gacctcgatc tttactggcg caaggacgtg agcgtccagt
     1321 tcgtcgagta tgccctgaag gaattgcgcg gcgacgacat gcgcgagacg cggctcaaga
     1381 ccgcgcgcca cgtcctcgac ggctaggccg gcttgcggcg cgccggcagg ctgcgccggg
     1441 agacgagccc cgtgatccac ttcaccgagc gccgcgcgcg ctcggcgacc atcggcaggg
     1501 gaagctgccg gtagtcgtcg ttgaacacct cgaagctgta gtcgccgcgg tagcccatct
     1561 cgtcgagctc gcgcaccagc gccgcgacct tgtcgctgtg cacgccctcg ccgggaaaga
     1621 cgcgcgagtg gcgcgcggtc tcgatgcgct cttccgccga gcgcacttcc tgccagagga
     1681 aatcggacag ctgcaccagg aacaccttgc gcgggtcgac ctcctgcagc agctccagat
     1741 cggtgcggtt ggcgaggata tggaacgaat cgagcgcgag gccgagattg gcgcggtcgg
     1801 cctcggacac gatttcccag gcggcgggga actcgttgac gtggcggccc catgacagcg
     1861 cctcgtaggc gatgcggatg tccaggggca ccgccagcat ggcgagtttc tgcaggtcct
     1921 tcttgatcaa ggaaacatct ttggaggaat gcggtgacgc cgtggagcac gccaagagaa
     1981 ccttggaacc cagcgcatgg cacatctcca gcattgcctt cgccacgtcc accttgtagg
     2041 cgtgcagcgt gccggacagc ccctcgaagt cgcgcagcac ctggaagccg gtgacgcgca
     2101 ggccgctcgc gcgcaccgcg gccaccgccg cggcctcgcc gccgggatgg ccgacgatgt
     2161 cggtggcgtt gagcatgatc tgcgtgaagc ccgcctcgcg caccgcccgc agcttggcct
     2221 ctagcggccc ggccagggtg atggtgtcca tgccgaaatg atccagattc acaagctgct
     2281 gacaaccagc tcgaaagtca ctccgccgag gtagatctgc gtgagcgcgc ccttgtcgct
     2341 cggctgcacc gcgccgtggt cgacgaagac gacgccgcgc tcctgcagcg ccttcactgc
     2401 ctttggcaca tcggttgcgc ccaggccgac gcgcaccagc ccttcgttcc agcgcacgtc
     2461 ctcggcgccg ggcggcggct cgatcagctg cagatagaac ttgtggcagg ggctctcgag
     2521 cagcgtgccc ttgggcagca cgccgaaata ctggccgcgc ggcaggacct ggaagccgaa
     2581 caggctgcgg tagaagtcga gccagtcggc ggtgcgctcg ggcaggatcg cctgcaccac
     2641 gccgaaccag tgcaggccgg ccacggccgg cggctttggt ttggcatttg aatcaaatac
     2701 aaaatcgaca tcgtagatcg cgaagtcctg gtagcggtcg atgaagtaga tcaggctgtc
     2761 gccgacgccg tggatgcctg gaatgtgcag ctccatcgcc gcggcgcgcg tcggcatctc
     2821 ccatgcgccg aggtcgagcg aatgctggaa cgcctgcccc gcatcgcgca cccggaacgc
     2881 catggcg
//
LOCUS       DASYJA010000498         1914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1255700, whole genome shotgun sequence.
ACCESSION   DASYJA010000498 DASYJA010000000
VERSION     DASYJA010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255700"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..520
                     /locus_tag="VGO02_10850"
     CDS             290..520
                     /locus_tag="VGO02_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HEV7478221.1"
                     /translation="MGKVLVLVMLVVAAVWLVRRALRAPKKGEFAQKPPVDGELVSCA
                     RCGVHLPRAEARMQSERLYCSEEHARLGPGKS"
     gene            520..>1914
                     /locus_tag="VGO02_10855"
     CDS             520..>1914
                     /locus_tag="VGO02_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEV7478222.1"
                     /translation="MDAAAPYPELMLAARERTPDSFWTSLRYFNLYRISLAALFFTSS
                     LVYGSELILGAYSIELFRYVALGYLFLAVVLHGVIRNVRDHFDIQLSLQAGLDILAII
                     LMMYASGGIRSGLGVMLLVSLTGAAIIAPRRLLYLYASLATIGLLLEQGYWVLSEDAP
                     TANFLQPALLAIGCFATAGVTSWLAQRVAANEALARSRGRALATQTRVNQLVIQDMHD
                     GVVVLDRDGRVVQHNPQAQRLLGAEHLAGASLAGLLTGFDAFWRGWRSGGAAASTADF
                     EARGRDLRLRLLDAATEEEFSVLFIEDTTRERDQAQQLKLAALGRLTANIAHEIRNPL
                     AAISHASELLGEEKRSKDLGRLTRIIHDNTRRLERLVADVLQLNRRDRISAERISLAP
                     WLAGFIVEFAANEALPAERLALEATARAAWIEFDREHLRQVVWNLLKNAVRHARAEPR
                     AVRLVIGAYADRIEL"
BASE COUNT          273 a          687 c          658 g          296 t
ORIGIN      
        1 ttgaatcttc ccacctctgc gaatacttcc ctgccgcgaa gcgcgatgct gacctcgaat
       61 ccggcgtgct gcagcagcag cctggtggtc acgacgtgct ccacgttatc gtccaccacc
      121 agcacccgca gcggccgtcc agcctgtcct gttgctccgt tcataaggtc agccgaacga
      181 atgttaccca aagacagcca tgggtccgtg cgccgcccgg accgtgcgtc gcggtgcgcg
      241 gcttgctagc ctcgcggccg gaagggacct ctgggtagat aatccgggca tgggcaaggt
      301 cctggtcctc gtaatgctcg tcgtggcggc ggtttggctg gtgcgccgcg cgctgcgcgc
      361 gccgaaaaag ggcgagttcg cgcaaaagcc gccggtcgac ggcgagctgg tgagctgcgc
      421 gcgctgcggg gtgcacctgc cgcgcgccga ggcgcgcatg cagagcgagc ggctctattg
      481 cagcgaagag cacgccaggc tcgggccggg gaaaagctga tggacgccgc ggcgccctac
      541 ccggaactga tgctcgcggc gcgcgagcgc acgccggact cgttctggac ctcgctgcgc
      601 tatttcaacc tctatcgcat ctcgctcgcg gcgctgttct tcacctcgtc gctggtctac
      661 ggcagcgagc tgatcctcgg cgcgtactcg atcgagctgt tccgctacgt cgcgctcggt
      721 tacctgttcc tcgcggtggt gctgcacggc gtgatccgca atgtgcgcga ccacttcgac
      781 atccagctgt cgctgcaggc cggcctcgac attctcgcca tcatcctcat gatgtacgcg
      841 agcggcggca tccgcagcgg cctgggcgtg atgctgctgg tatcgcttac cggcgccgcg
      901 atcatcgcgc cgcgccggct gctctacctg tacgcgtcgc tcgccaccat cgggctgctc
      961 ctcgagcagg gctactgggt gctgtccgag gacgcgccga ccgccaactt cctgcagccg
     1021 gcgctgctcg ccatcggctg cttcgccacg gccggggtga ccagctggct cgcgcagcgc
     1081 gtcgccgcga acgaggcgct ggcgcgcagc cgcggccgcg ccctcgccac gcagacgcgc
     1141 gtcaaccagc tggtgatcca ggacatgcac gacggcgtgg tggtgctcga ccgcgacggc
     1201 cgcgtggtgc agcacaaccc ccaggcgcag cgcctgctgg gcgccgagca cctggccggg
     1261 gcgagcctgg ccgggctgct gaccgggttc gacgccttct ggcgcggctg gcgctccggc
     1321 ggcgcggcgg cgagcaccgc cgacttcgag gcgcgcggcc gcgacctgcg cctgcggctg
     1381 ctcgacgccg ccaccgagga ggagttctcg gtgctgttca tcgaggacac gacgcgcgag
     1441 cgcgaccagg cgcagcagct caagctcgcg gcgctcggcc ggctgaccgc caacatcgcg
     1501 cacgagatcc gcaatccgct ggcggcgatc agccacgcct cggagctgct cggcgaggag
     1561 aagcgcagca aggacctcgg gcgcctgacg cgcatcatcc acgacaacac gcggcgcctc
     1621 gagcggctgg tggccgacgt gctgcagctc aaccgccgcg accgcatctc ggcggagcgc
     1681 atcagcctgg cgccctggct cgccggcttc atcgtcgagt tcgcggccaa cgaggcgctg
     1741 ccggccgagc gcctggcgct cgaggcgacg gcgcgcgcgg cctggatcga gttcgaccgc
     1801 gagcacctgc gccaggtggt gtggaacctg ctgaagaacg ccgtgcgaca cgcgcgcgcc
     1861 gagccgcgcg cggtgcgcct ggtcataggc gcctacgccg accgcataga attg
//
LOCUS       DASYJA010000499         1981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_530740, whole genome shotgun sequence.
ACCESSION   DASYJA010000499 DASYJA010000000
VERSION     DASYJA010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530740"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /gene="mraW"
                     /locus_tag="VGO02_10860"
     CDS             <1..332
                     /gene="mraW"
                     /locus_tag="VGO02_10860"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HEV7478223.1"
                     /translation="EVSLMLPQALERLASAGRLAIIAFHSLEDRIVKRFLQQAARPEM
                     PRRLPLRASEMPQPSLKIIGRAVKASSEETRSNPRARSAILRVAERTAAPYAPNIRIE
                     PDARWRN"
     gene            320..592
                     /gene="ftsL"
                     /locus_tag="VGO02_10865"
     CDS             320..592
                     /gene="ftsL"
                     /locus_tag="VGO02_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEV7478224.1"
                     /translation="MAKLNLLLLAVLVACAIGLVTSQHRARKLFSDLEREQARARALD
                     VEYGQLQLEASTWALHTRVETIAARKLGLRAPDPRRVRVVRPESGR"
     gene            589..>1981
                     /locus_tag="VGO02_10870"
     CDS             589..>1981
                     /locus_tag="VGO02_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEV7478225.1"
                     /translation="MNAARQANAGLVRLPVWRARFVATALVGAFVVLAGRSLYLQTLR
                     TDFLQEKGDARSARVIEMPATRGRILDRNGDALAVSTPVKSIWALPGDVEFAPGQKKK
                     LAALLAMEPREIDKKLADPSRDFVYLKRQVAPETADAVAALGLKGIHQQREYRRYYPG
                     GEVTAHVVGFTGVDDAGQEGIELAHQATLGGQPGSRRVIKDRLGRIVEDVESIRAGQD
                     GADLTLSIDGKIQSLAYSTLKAAVEENRAKAGALIAIDVRTGEILALANYPSFNPNNR
                     ARLSGAQLRNRVITDLFEPGSTLKPFTIALALETGKVTPATMVHTAPGRLTIANYTIH
                     DVHPSAALSLSQVIQKSSNVGAARLALAMPSEAMWDMFQRVGFGAPPQLGFPGAAAGR
                     LRPYKTWRPIEQATMAYGHGISVSLAQLARAYTVFARDGELVPLTLVKTNAEAVGEKV
                     LSAASAQAVRAMLE"
BASE COUNT          319 a          709 c          664 g          289 t
ORIGIN      
        1 aggaagtgtc gctgatgctc ccccaggcgc tcgagcggct ggcgagcgcc gggcgactgg
       61 cgatcatcgc cttccattct ctcgaagatc gcatcgtcaa gcgcttcctg cagcaggcgg
      121 cgcgccccga gatgccacgg cgtttgccgc tgcgtgccag cgaaatgcca caaccctccc
      181 tcaagatcat cggccgggcg gtcaaggcat cgtctgaaga aaccagaagc aacccgcgcg
      241 cgcgcagtgc catcctcagg gtggccgagc gcacggcggc gccctatgca ccgaacattc
      301 gaatcgagcc cgacgcgcgg tggcgaaact gaacctcctc ctcctcgcgg ttctcgtcgc
      361 ttgcgcgatc gggctcgtca cgtcccagca ccgggcacgc aagctgttct cggacctcga
      421 gcgcgagcag gcgcgcgcgc gcgccctcga cgtcgagtac ggccagctgc agctcgaggc
      481 gagcacctgg gctttgcata cgcgcgtcga gaccatcgcc gcccgcaagc tcggcctgcg
      541 ggcaccggat ccgcgccgcg tgcgtgtcgt gcggccggag agcggacggt gaacgcggcc
      601 aggcaggcaa acgcgggcct cgtgcgcctg ccggtgtggc gcgcgcgctt cgtcgccacg
      661 gcgctggtcg gcgcgttcgt cgtgctcgcc ggccgctcgc tttacctgca gaccctgcgc
      721 accgatttcc tgcaggagaa gggcgacgcg cgctctgcgc gggtcatcga gatgccggcg
      781 acgcgcgggc gcatcctcga ccgcaacggc gatgccctcg ccgtatcgac gccggtgaaa
      841 tcgatctggg cccttccggg cgacgtcgag ttcgctcccg gccagaaaaa gaagctcgcc
      901 gcgctgctcg ccatggagcc tcgcgagatc gacaagaagc tggccgaccc gtcgcgcgac
      961 ttcgtctatc tgaagcgcca ggtggcgccc gaaaccgccg atgcggtggc ggcgctcggc
     1021 ctgaagggca tccaccagca gcgcgagtac cggcgttact accctggtgg cgaagtgacc
     1081 gctcacgtgg tgggcttcac cggggtcgac gatgccggcc aggaaggcat cgagctcgcc
     1141 catcaggcga cgctgggcgg ccagccgggc agccggcgcg tcatcaagga tcggctcggt
     1201 cgcatcgtcg aggacgtcga atcgatccgc gccgggcagg acggcgccga cctcaccctg
     1261 tccatcgacg gcaagatcca gagcctcgcc tacagcaccc tcaaggcggc ggtggaggaa
     1321 aaccgcgcca aggcgggcgc gctgatcgcg atcgacgtgc gcaccggcga gatcctggcg
     1381 ctcgccaact acccttcctt caatccaaac aaccgcgccc gccttagcgg ggcgcagctg
     1441 cgcaaccggg tgatcacgga cctcttcgag ccgggctcga ccctcaagcc tttcacgatc
     1501 gcgctcgcgc tggagaccgg caaggtgacg ccggcgacca tggttcacac cgcgcccggt
     1561 cgtctcacga tcgcgaacta cacgatccat gacgtgcatc cgtcggcggc gctcagcctg
     1621 tcgcaggtga tccagaagtc gtccaacgtc ggcgcggcga ggctcgccct cgccatgccg
     1681 agcgaggcaa tgtgggacat gttccagcgc gtcggcttcg gcgctccgcc gcagctcggc
     1741 tttccggggg ccgcggcggg ccggctgcgc ccctacaaga cctggcggcc gatcgagcag
     1801 gcgaccatgg cctacggcca cggcatctct gtcagcctgg cgcagctcgc gcgcgcctac
     1861 accgtgttcg cgcgcgatgg ggaattggtg ccgctcacgc tggtcaaaac gaatgccgag
     1921 gcggtcggcg agaaggtgct gtccgccgca tcggcccagg cggtacgcgc catgctcgag
     1981 c
//
LOCUS       DASYJA010000500         1603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1346132, whole genome shotgun sequence.
ACCESSION   DASYJA010000500 DASYJA010000000
VERSION     DASYJA010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346132"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VGO02_10875"
     CDS             <1..358
                     /locus_tag="VGO02_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEV7478226.1"
                     /translation="YLERYPSLDLLDTVFQQTLETKGPEDAYRLVRDELRRNPTLLGL
                     DRLLEVQIIGATSPERRHDLELMRNLVHSHTQRLARYRCEGCGFKARQFHWRCPACGG
                     WETYPPRRTEEFELTP"
     gene            355..>1603
                     /locus_tag="VGO02_10880"
     CDS             355..>1603
                     /locus_tag="VGO02_10880"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEV7478227.1"
                     /translation="MNVSIIGTGYVGLVTGACLADAGNHVVCLDIDHDKVSQLNAGKI
                     PIFEPGLEPVVRRNREAGRLRFSTDVAASVAHGELQMLAVGTPSGEDGSADLQHVLAA
                     ARNVGRHMESWKAIVTKSTVPVGTADKIRAAVIEELEKRKSAVEFAVVSNPEFLKEGA
                     AVQDFMRPDRIIIGADDPRAMALLRQLYAPFQRNHERVMTMGVRSAELTKYAANSMLA
                     TRISFMNEIANLAEALGADIEEVRRGIGSDPRIGYHFLYPGAGFGGSCFPKDVRALQR
                     SAIEAGRPLRVLAAVDQVNDSQKHVLGDKIRARFGANLSGKRFALWGLAFKPNTDDMR
                     EAPSRTVIADLIGAGATVVRAYDPVAANEARKIFAHENRLRIVDSALDALEGADALAI
                     VTEWQEFRSPDFEAIKAKLKTPAI"
BASE COUNT          266 a          581 c          538 g          218 t
ORIGIN      
        1 ctacctggag cgctatcctt cgctcgacct gctcgacacc gtgttccagc agacgctcga
       61 gaccaaggga cccgaggacg cctaccgcct ggtgcgcgac gagctgcggc gcaacccgac
      121 gctgctcggc ctggaccggc tgctggaagt ccagatcatc ggcgccacct cgcccgagcg
      181 caggcacgac ctcgagctga tgcgcaacct ggtccacagc cacacgcagc gcctcgcccg
      241 ctaccgctgc gagggctgcg gcttcaaggc gcgccagttc cattggcgct gtcccgcctg
      301 cggcggctgg gaaacctacc cgccgcggcg caccgaggag ttcgagctca cgccatgaac
      361 gtcagcatta tcggaacggg gtacgtgggg ctggtgacgg gcgcctgcct ggccgatgcc
      421 ggcaaccacg tcgtctgcct ggacatcgac cacgacaagg tatcccagct caacgcgggc
      481 aagatcccga ttttcgagcc aggcctggag ccggtggtgc ggcgcaaccg cgaggccggc
      541 cgcctgcgct tcagcaccga cgtcgccgcg agcgtggcgc acggcgagct gcagatgctc
      601 gccgtcggca cgccctcggg cgaggacggc tcggccgacc tgcagcacgt gctggccgcg
      661 gcgcgcaacg tcggccgcca catggaatcg tggaaggcga tcgtcaccaa gtcgaccgtg
      721 ccggtgggca ccgccgacaa gattcgcgcc gcggtgatcg aggagctgga gaagcgcaag
      781 agcgcggtcg agttcgccgt ggtctccaac ccggagttcc tcaaggaggg cgccgcggtg
      841 caggacttca tgcgcccgga ccgcatcatc atcggcgccg acgacccgcg cgcgatggcg
      901 ctgctgcgcc agctctacgc gccgttccaa cgcaaccacg agcgcgtgat gacgatgggc
      961 gtgcgctcgg ccgagctaac caagtacgcc gccaactcga tgctggcgac gcgcatctct
     1021 ttcatgaacg agatcgccaa cctggccgag gcgctcggcg ccgacatcga ggaggtgcgc
     1081 cgcggcatcg gctccgaccc gcgcatcggc taccacttcc tctaccccgg cgccggcttc
     1141 ggcggctcgt gcttcccgaa ggacgtgcgc gcgctgcaaa ggagcgcgat cgaggccggc
     1201 cggccgctgc gcgtgctcgc cgcggtggat caggtgaacg actcgcagaa gcacgtgctc
     1261 ggcgacaaga tccgcgcgcg cttcggcgcc aacctctcgg gcaagcgctt cgcgctgtgg
     1321 gggctcgcct tcaagccgaa caccgacgac atgcgcgagg cgcccagccg cacggtgatc
     1381 gccgacctga tcggcgctgg cgccaccgta gtgcgcgcct acgacccggt cgcggcgaac
     1441 gaggcgcgca agatcttcgc ccacgagaac cgcctgcgca tcgtcgactc ggcgctcgac
     1501 gcgctggagg gggccgacgc gctcgccatc gtcaccgagt ggcaggagtt ccgcagcccg
     1561 gacttcgagg cgatcaaggc caagctcaag accccggcca tct
//
LOCUS       DASYJA010000501         1550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3072751, whole genome shotgun sequence.
ACCESSION   DASYJA010000501 DASYJA010000000
VERSION     DASYJA010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3072751"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1550)
                     /locus_tag="VGO02_10885"
     CDS             complement(<1..>1550)
                     /locus_tag="VGO02_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006760308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEV7478228.1"
                     /translation="ERDPDKLVEIIASLEPTFGGINLEDIKAPECFIVERKLRARMKI
                     PVFHDDQHGTAITVAAAIVNGLKVVKKDIQEVKLVASGAGAAALACLELLVKMGLPLA
                     NIMVTDLKGVVYKGRREEMDPDKERFAKDTRARTLDEVIAGADVFLGLSAGGVLKPAM
                     VKKMAERPLILALANPEPEILPELAKEARPDCVIATGRSDYPNQVNNVLCFPFIFRGA
                     LDVGATTITTEMELAAARAIAELAQAETSEQVALAYGIETINFGADYIIPKPFDPRLI
                     ARIAPAVAQAAMDSGVATRPIADLEAYRQSLTQFVYHSGLIMKPLFTAAKAAPRRIVY
                     AEGEDERVLRAAQIAVDEGLARPILIGRPQVIEQRLERLGLRVRPGKEFDLVNPEFDA
                     RYRDYWTTYHRLSERKGVSPEYARIEMRRRQTLIGAMLMYKNEADGMLCGTFGTHAQH
                     LLFVDQVIGRRAGVAHYSAMNVVMLPARTVFICDTYVTDDPDAEHVAEMTLLAAEAVR
                     RFGIAPKV"
BASE COUNT          215 a          532 c          538 g          265 t
ORIGIN      
        1 caccttgggg gcgatgccga agcggcgtac tgcctcggcg gcgagcagcg tcatctcggc
       61 gacgtgctcg gcgtcggggt cgtcggtcac gtaggtgtcg cagatgaaga cggtgcgcgc
      121 cggcagcatc accacgttca tcgccgagta gtgggccacc ccggcgcgcc ggccgatcac
      181 ctggtcgacg aagagcaggt gctgggcgtg cgtgccgaag gtgccgcaca gcatgccgtc
      241 ggcctcgttc ttgtacatca gcatcgcgcc gatcagcgtt tgccggcgcc gcatctcgat
      301 gcgcgcgtat tccggcgaca cgcccttcct ctcggagagg cggtggtagg tcgtccagta
      361 gtcgcggtag cgcgcgtcga actccgggtt caccaggtcg aattccttgc ctgggcgcac
      421 gcgcaggccg aggcgctcca ggcgctgctc gatcacctgc gggcggccga tcaggatcgg
      481 gcgcgccagg ccctcgtcca ccgcgatctg cgcggcgcgc agcacgcgct cgtcctcgcc
      541 ctcggcgtag acgatgcgcc gcggcgcggc cttggccgcc gtgaacagcg gcttcatgat
      601 caggccggag tggtagacga actgggtcag cgactgccgg taggcctcga ggtcggcgat
      661 cggccgcgtc gccacgcccg agtccatcgc cgcctgcgcc accgccggcg cgatgcgcgc
      721 gatcaggcgc gggtcgaacg gcttggggat gatgtagtcg gcgccgaagt tgatggtctc
      781 gatgccatag gcgagcgcca cctgctccga cgtctctgcc tgggcgagct cggcgatggc
      841 gcgcgccgcg gcgagctcca tctcggtggt gatggtggtg gcgccgacgt ccagcgcgcc
      901 gcgaaagatg aacgggaagc agaggacgtt gttcacctgg ttcgggtagt ccgagcgccc
      961 ggtggcgatg acgcagtccg ggcgcgcctc cttcgccagc tccggcagga tctccggctc
     1021 ggggttggcg agcgccagga tcagcggccg ctcggccatc ttcttcacca tcgccggctt
     1081 cagcaccccg ccggccgaca ggccgagaaa cacgtcggcg ccggcgatca cctcgtccag
     1141 cgtgcgcgcc cgggtgtcct tcgcgaagcg ctccttgtcc gggtccatct cctcgcgccg
     1201 gcccttgtac accacgccct tgaggtcggt gaccatgatg ttggcgagcg gcaggcccat
     1261 cttcaccagc agctccaggc aggcgagcgc ggcggcgccg gcgcccgagg cgaccagctt
     1321 cacttcctgg atgtccttct tgaccacttt gagcccattg acgatcgccg cggccacggt
     1381 aatcgcggtg ccgtgctggt cgtcgtggaa caccgggatc ttcatgcgcg cgcgcagctt
     1441 gcgctccacg atgaagcatt cgggcgcctt gatatcctcc aggttgatgc cgccgaaggt
     1501 cggctccagg ctggcgatga tctccaccaa tttgtccggg tcgcgctcgt
//
LOCUS       DASYJA010000502         2092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_257288, whole genome shotgun sequence.
ACCESSION   DASYJA010000502 DASYJA010000000
VERSION     DASYJA010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257288"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="VGO02_10890"
     CDS             complement(<1..1037)
                     /locus_tag="VGO02_10890"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV7478229.1"
                     /translation="MDGQVLSWSPQALGEVLERQARERGGAEALVTQAGRFSFEEMFQ
                     RAKDVAGGMQSLGIRSGDHVGILMGNDERWLSLFYGAALIGAVTVPVNTRFKAAEIDF
                     CLKQAECKALFYVEQFLGIDFGAVVREIGFRNALPVLPSGRFSPVPVAPGKILLIQFT
                     SGTTAYPKGAMLTHDNMLRDAWHAGSRIGIRAEDRYFNCRPFFHVAGSTLSALMALAA
                     GACLVTLPTFEAGAALELMERERCTLVSGNDTLFQMLMGHADFAKRKLSLRGGWAAAG
                     PQTMRSIIDVLGAKHICAAYGLSEASPNVVMSDWRDAEELRIQGLAKPHEGVELRIVD
                     NEIQVRGWNVMR"
     gene            complement(1019..1735)
                     /locus_tag="VGO02_10895"
     CDS             complement(1019..1735)
                     /locus_tag="VGO02_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEV7478230.1"
                     /translation="MKSILYSVEAGVATIMLDRPEARNALNGAMCDALCEAAARAAAD
                     TSVRLVLVRGNGPVFCAGADLKERQGMGKDEVRARRLKGFAAYSALEALPMPVIAIVQ
                     GAALGSGVEIAAACDFIVALPDAVFGTPEALRGTVGATQRLPRVLGKRLAKDLMFTGR
                     RLDAQEALAAGFVSRVSQDVEREIAEITKTILAAPPMAMRLAKRCIDRGVELDPQGAL
                     DTEVEAIEEQLASGQWMGKS"
     gene            complement(1732..>2092)
                     /locus_tag="VGO02_10900"
     CDS             complement(1732..>2092)
                     /locus_tag="VGO02_10900"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV7478231.1"
                     /translation="GLEQWGLPFNSNAVRVEKRDEVMSVLTREIEKLSRAELLSKLDE
                     ADVPVGPVNDVEEILSDPHVRARKLVGSFDYPGVGEFRALALPYKFLGWDNPQIGRPP
                     TLGEHTGKILAELKAGK"
BASE COUNT          335 a          741 c          658 g          358 t
ORIGIN      
        1 cgcatcacgt tccagccccg cacctggatc tcgttgtcga cgattctcag ctcgacgccc
       61 tcgtgcggct tggccagtcc ctggatgcgc aattcctcgg cgtcgcgcca gtcgctcatg
      121 accacgttgg gcgaagcctc agacaatccg taggcggcgc agatgtgctt tgcgcccagc
      181 acgtcgatga tcgagcgcat ggtctgcggc ccagccgccg cccagccgcc gcgcaggctg
      241 agcttgcgct tcgcgaagtc ggcgtggccc atcagcatct ggaacagcgt gtcgttgccc
      301 gagaccagcg tgcagcgctc gcgctccatc agctcgagcg ccgcgccggc ctcgaaggtc
      361 ggcagcgtga ccaggcaggc gccggccgcg agcgccatca gcgccgacag cgtcgagccg
      421 gcgacatgga agaagggccg gcagttgaag tagcggtcct cggcgcggat gccgatgcgg
      481 ctgccggcgt gccaagcgtc gcgcagcatg ttgtcgtgcg taagcatcgc acccttggga
      541 taggccgtcg ttcccgaggt gaattgaatc aggaggatct tgcccggcgc cacgggcacg
      601 gggctgaagc gccctgaggg cagcaccgga agagcgttcc tgaacccgat ctcgcggacc
      661 acggcgccga aatcgattcc cagaaattgc tcgacgtaaa acagcgcctt gcattcggcc
      721 tgcttcaggc agaagtcgat ctccgccgcc ttgaagcgcg tgttgaccgg gaccgtgacc
      781 gcgccgatga gcgccgcgcc gtagaagagc gaaagccagc gctcgtcgtt gcccatcagg
      841 atgccgacat ggtcgccgct gcggatgccg agcgattgca tgccccccgc gacgtccttc
      901 gctcgctgga acatttcctc gaaagaaaat cttcccgcct gcgtcaccag tgcctcggcg
      961 ccgccccgct cgcgcgcctg gcgctcgagc acttcgccga gggcttgcgg cgaccagctc
     1021 aggacttgcc catccattgg cccgaggcga gctgctcttc gatcgcctcg acctcggtgt
     1081 cgagcgcgcc ctgcgggtcg agctccacgc cgcggtcgat gcagcgcttg gcgaggcgca
     1141 ttgccatggg cggtgcggca agaatggttt ttgttatttc ggcaatttct ctttccacat
     1201 cctgtgagac tcgagaaaca aatcccgcag ccagcgcctc ctgcgcgtca aggcgcctgc
     1261 cggtgaacat caggtccttc gccaggcgct tgccgaggac gcgcggcagc cgctgcgtcg
     1321 cgcccacggt gccgcgcagc gcctcgggcg tgccgaacac cgcgtcgggc agcgcgacga
     1381 tgaagtcgca ggccgccgcg atctcgacgc cggagccgag cgcggcgccc tgcactatcg
     1441 cgatcaccgg catcggcagc gcttcgagcg cactgtaggc ggcgaagccc ttgaggcgcc
     1501 gcgcgcgcac ctcgtccttc cccatgccct ggcgctcctt caggtcggcg ccggcgcaga
     1561 acaccggccc gttgccgcgc accaggacca ggcgcacgct cgtgtccgcc gcggcgcgcg
     1621 ccgccgcctc gcacagcgcg tcgcacatcg cgccgttcag cgcgttgcgc gcctccggcc
     1681 ggtcgagcat gatcgtcgcg acgccggctt cgacgctgta gaggatgctc ttcacttgcc
     1741 cgctttcaac tccgccagga tcttcccggt gtgctcgccg agcgtcggcg gccggccgat
     1801 ctgcgggttg tcccagccga ggaacttgta gggcagcgcg agcgccctga attcgcccac
     1861 gcccggatag tcgaagctgc cgaccagctt gcgcgcgcgt acatgcggat cgctcaggat
     1921 ttcttcgaca tcgttcaccg gaccaactgg aacatctgcc tcatccaact tcgacaatag
     1981 ctccgcacga ctcagctttt cgatttccct ggtgagaacc gacatgactt cgtcgcgctt
     2041 ttcgacccgg acagcgttcg aattgaaagg caagccccat tgttcgagcc ca
//
LOCUS       DASYJA010000503         1609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_75485, whole genome shotgun sequence.
ACCESSION   DASYJA010000503 DASYJA010000000
VERSION     DASYJA010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75485"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /gene="yidC"
                     /locus_tag="VGO02_10905"
     CDS             complement(<1..1085)
                     /gene="yidC"
                     /locus_tag="VGO02_10905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV7478232.1"
                     /translation="MDTQRLILLFIFSFSLLMLWEAWDKQGRPKPVPPAPAAQGVPAP
                     AKPAAPETAGKAAAPGAPAAGTVPGAEPTAKGETVRVTTDLLVAEIDTLGGTLKRVEL
                     LHHKDSKDPTKPFVLLGPDHQYEAQSGLTGDAGPNHRTLWREQPGERTLGPGRDAVEL
                     RLSARGKDGLEVTKIYTFKRDSYVIDVALELKNGGGAPVSTYSYFQLTHDGKPDVPTN
                     SEASTFGAQSFIGFGAYTNEHKYQKIHLSDLDKGKSDHVKQADNGWLAFVQHYFVSAW
                     LPTDGTPRDYVTERRPDGTYAGRLMISATVAPGASALVSAPLYAGPQEQKRLRTAAPG
                     LDLVVDYGFLAIIAWPLFWLLEKYHAL"
     gene            complement(1093..1305)
                     /gene="yidD"
                     /locus_tag="VGO02_10910"
     CDS             complement(1093..1305)
                     /gene="yidD"
                     /locus_tag="VGO02_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEV7478233.1"
                     /translation="MSARLLLGVVRAYQWCVRPVLPRTCRFHPNCSNYTHETLTRHGA
                     WRGGLLSAWRVCRCGPWHPGGYDPVP"
     gene            complement(1302..>1609)
                     /gene="rnpA"
                     /locus_tag="VGO02_10915"
     CDS             complement(1302..>1609)
                     /gene="rnpA"
                     /locus_tag="VGO02_10915"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEV7478234.1"
                     /translation="TQKARFERLLRDGRVKKVAGYTFYLESRDAGDPRLGLLVTRRHA
                     ARASDRNRIKRCVREAFRLEQGRLGAVDLLVRPPYGVKPSPLMIARLRDIFATLSWA"
BASE COUNT          257 a          513 c          561 g          278 t
ORIGIN      
        1 ctgagggcgt ggtacttctc cagcagccag aagaggggcc aggcgatgat ggcgagaaac
       61 ccgtagtcga ccaccaggtc gagcccgggt gccgccgtgc gcaggcgctt ctgctcctgc
      121 ggtccggcgt agagcggcgc cgagaccagc gccgacgcgc ccggcgcgac ggtcgcggaa
      181 atcatcaacc tgccggcgta ggttccgtcg gggcgcctct cggtcacgta atcgcgtggc
      241 gtgccatcgg tcggcagcca ggccgacacg aaatagtgct gcacgaaagc gagccagccg
      301 ttgtcggcct gcttcacgtg gtcggattta cccttgtcga gatccgagag gtgaatcttc
      361 tggtacttgt gctcgttggt ataggcgccg aagccgatga agctctgcgc gccgaaggtc
      421 gacgcctcgc tgttggtggg gacatcgggt ttgccgtcgt gggtcagctg aaagtaggag
      481 taggtcgaaa ccggcgcgcc tccgccgttt ttcagctcga gcgcaacgtc gatcacgtag
      541 ctatcgcgct tgaaggtata gatcttggtg acctccaacc cgtccttgcc gcgagccgag
      601 aggcgcagct cgacggcgtc ccggcccggc cccaaggtgc gctcgcccgg ctgctcacgc
      661 cacagcgtcc tgtggttcgg tccggcgtcc ccagtgagcc cgctttgggc ttcgtattgg
      721 tggtccggcc ccagaagcac gaaaggcttg gtcggatcct tcgaatcctt gtggtggaga
      781 agctcgacgc gcttcagcgt gccgccgagg gtgtcgatct cggcgaccaa gagatctgtc
      841 gtcacgcgaa ccgtctcgcc cttcgcagtg ggctccgcac cgggaacggt ccccgctgcg
      901 ggtgcgccgg gtgcggccgc cttgcccgcg gtctcggggg cggcgggctt tgcgggagcg
      961 ggcacaccct gcgccgcggg cgcgggaggg accggcttgg gccgcccctg cttgtcccaa
     1021 gcctcccaca gcatcagcag cgaaaagctg aagatgaaga gcaggatgag tctttgggtg
     1081 tccattttca cttcagggca ccgggtcgta gccgcctggg tgccagggac cgcagcggca
     1141 aacgcgccag gcgctcagca ggccgccgcg ccaggcgccg tggcgggtga gggtttcgtg
     1201 cgtgtaattc gagcagtttg gatggaagcg gcaggttcgc ggtagaaccg ggcgcacgca
     1261 ccactggtac gcccgcacca cgccaagcag aaggcgggca ctcatgccca gctcaacgtc
     1321 gcgaagatgt cgcgcaggcg cgcgatcatc agcggcgagg gcttgacgcc gtagggcggc
     1381 cgcaccagca ggtcgactgc gcccagtcgg ccttgttcaa ggcgaaacgc ctcgcgcacg
     1441 cagcgcttga tgcggttgcg gtcgctcgca cgggcggcat gccggcgggt cacaagcagc
     1501 ccgaggcgcg gatcgcccgc atcgcggctt tccaggtaga aggtatagcc agccactttc
     1561 tttaccctgc cgtcgcgcag caggcgttcg aatcgcgctt tttgcgtga
//
LOCUS       DASYJA010000504         2120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2345791, whole genome shotgun sequence.
ACCESSION   DASYJA010000504 DASYJA010000000
VERSION     DASYJA010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2345791"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="VGO02_10920"
     CDS             complement(<1..1138)
                     /locus_tag="VGO02_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEV7478235.1"
                     /translation="MTVASVASRALAGIEAPEVTVEVHLGPGLPAFHIVGLPDAEVRE
                     ARDRVRAALNHARFEFPARRITVNLAPAELPKDSSRFDLPIALGILAASGQLAPEALC
                     ELEFAGELSLTGELRAVRGALAMTLAARATGRAFVLPAGNAPQAALAKGVRVLPARTL
                     LEVVAHISGEAPLAQHPPSIPVQKNSYGDFSDVKGQQQVKRALEVAAAGAHSVLMLGP
                     PGTGKSMLAARFPGILPPLSEAEALEVAAIHSVSSAGFEAARWGERPFRAPHHSASGP
                     SLVGGGNLPRPGEISLAHHGVLFLDELPEFDRDVLEALREPLGTGRVCISRAARQALF
                     PARFQLVAAMNPCPCGHCGDRSGRCRCTPERIARYRGRISGPFAD"
     gene            complement(1165..1407)
                     /locus_tag="VGO02_10925"
     CDS             complement(1165..1407)
                     /locus_tag="VGO02_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEV7478236.1"
                     /translation="MIDKRFFDELNERIGEAFRNSPAQDIEKNLKAMLASGFARLDLV
                     LREDFEVQQKLLERAQQKLAELEARIAELEGKRDKG"
     gene            1584..>2120
                     /locus_tag="VGO02_10930"
     CDS             1584..>2120
                     /locus_tag="VGO02_10930"
                     /inference="COORDINATES: protein motif:HMM:NF021230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEV7478237.1"
                     /translation="MRKSVLFAAVATALAVPTLVAAQTPAAAPPPVTGNMTIASDYRF
                     RGISQTYTGPTIQGGIDYAHASGLYLGNWNSNVSSVLFAGGSGIEMDFYGGWKKSFGD
                     FGLDVGTIYYHYPNAEFNTNTGSAGGSSKFENWEIYIGGSWKWISAKFFYALDNYFGL
                     DNVQAGEGFFVNKNTGALL"
BASE COUNT          369 a          712 c          699 g          340 t
ORIGIN      
        1 ggtcggcgaa cggcccggag atgcggccac ggtagcgcgc gatgcgctcg ggcgtgcagc
       61 ggcagcggcc gctgcggtcg ccgcagtggc cgcaggggca gggattcatg gccgcgacga
      121 gctggaagcg cgccgggaag agcgcctgtc gggcggcacg cgagatgcag actcggccgg
      181 tcccgagcgg ctcgcgaagg gcctcgagca catcgcggtc gaattcggga agctcatcca
      241 ggaacagcac cccgtgatgt gcgagggaga tctcccccgg acgcggcagg tttcccccgc
      301 cgacgagcga gggcccggag gcactgtggt gcggggcgcg aaagggccgc tcgccccagc
      361 gcgcggcctc gaagccggca ctggagaccg agtggatcgc ggcaacttcc agcgcctcgg
      421 cctcggaaag cggcggcagg atgccgggaa agcgcgccgc cagcatggac ttgccggtcc
      481 cgggcggtcc gagcatcagc acgctgtgcg cgccggccgc cgcaacttcg agggcacgct
      541 tgacctgctg ctgacccttc acgtcgctga aatcgccgta cgagttcttc tgaaccggaa
      601 ttgaaggggg atgctgggca agcggcgctt ccccagagat gtgagccacc acttcgagca
      661 gcgtgcgcgc cggcaggacc cgcacaccct tcgccaaggc ggcctgcggc gcattgcccg
      721 cgggcaggac gaaggcccgt ccggtcgcgc gcgcggccaa cgtcatcgca agcgccccac
      781 gcaccgcgcg aagctcgccg gtcagcgaaa gctccccggc gaactcgagc tcgcacaagg
      841 cttcgggtgc gagctggccg ctcgccgcga ggatgccgag cgcgatcggc agatcgaagc
      901 ggctggagtc cttcggcagc tccgcgggcg ccaggttgac cgtgatgcgg cgcgcgggaa
      961 actcgaatcg cgcgtgattc aacgccgcgc gcacccggtc gcgcgcctcg cgtacctcgg
     1021 cgtccggaag tccgacgatg tgaaatgcgg gcagtccggg cccgagatgc acttcgaccg
     1081 tcacctcggg cgcttcgatt cccgcgagcg ctcggctcgc gacgctcgcc acagtcatcg
     1141 tgttcccccc gcaggaggaa cgtttcagcc cttgtcgcgt ttaccttcga gctccgcgat
     1201 gcgcgcttcg agctcggcga gcttctgctg agcccgctcg aggagcttct gctgtacctc
     1261 gaagtcctcg cgcagcacga ggtcgagccg cgcgaacccc gaggcgagca tcgccttcag
     1321 gttcttctcg atgtcctgcg cgggcgaatt gcggaacgcc tcgccgatgc gctcgttcag
     1381 ctcgtcaaaa aatcgcttgt cgatcatgga gagaggctac cgcaccgaag tcgagcgcgg
     1441 cgcgaccctg catgcaccag taaagtgcgc cgcgcggcct gttcctttcc aagtcattgt
     1501 taacaagtct gaaattcctt ggcataggag atgctaagcg tccggaggct atttctactc
     1561 accacatcgt ctagggagat ccgatgcgca agtcagtcct tttcgccgct gtagctaccg
     1621 ccctcgccgt accgacgctc gtcgccgccc aaaccccggc tgcagcgccg cctccggtga
     1681 ctgggaacat gaccatcgcg tcggactacc ggttccgcgg catatcgcag acctacaccg
     1741 gacccactat tcagggcggc atcgactacg cgcacgcgag cgggctgtac ctcggcaact
     1801 ggaactcgaa cgtgtcgagc gtcctgttcg ccggcggctc gggtatcgag atggacttct
     1861 acggcggctg gaagaagtcc ttcggcgact tcggtctcga tgtcgggacg atctattacc
     1921 actacccgaa cgctgaattc aacacgaata ccggcagcgc cggcggcagc tccaagttcg
     1981 agaactggga gatctacatc ggcggtagct ggaagtggat ttccgccaag ttcttctacg
     2041 cgctcgacaa ctacttcggc ctggacaacg tgcaggcggg cgagggcttc ttcgtgaaca
     2101 agaacaccgg cgcgctgctc
//
LOCUS       DASYJA010000505         3319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2163931, whole genome shotgun sequence.
ACCESSION   DASYJA010000505 DASYJA010000000
VERSION     DASYJA010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2163931"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VGO02_10935"
     CDS             complement(<1..834)
                     /locus_tag="VGO02_10935"
                     /inference="COORDINATES: protein motif:HMM:NF040071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HEV7478238.1"
                     /translation="MSLLVALAILVVPPALSPASAAPPTLEIKVLSNRADLISGGEAL
                     AEVVVPAGVLATSVRMDVGGRDVTSSFAVRANGRFMGRVTGLALGSNVLSARAKGASA
                     ASITVTNHPIGGPVFAGFQVQPWICETEANGFGPPQDAQCNVAARYEFFYKSTSPAAS
                     GLRAYDPGNPPLDVATTVTDQGHVVPFIVRQETGVIDRGWYRIGVLFDPAQPWEPWEP
                     QRGWNGKIVWPFGGSSASDHYQNSPPSVLQEERLGKGFMVAASSLNTHGQNNNDVVSS
                     EA"
     gene            complement(990..1580)
                     /gene="wrbA"
                     /locus_tag="VGO02_10940"
     CDS             complement(990..1580)
                     /gene="wrbA"
                     /locus_tag="VGO02_10940"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEV7478239.1"
                     /translation="MADILVLYYSVGGSVRRMAELVAEGIERVPGAAARLRTVPRVST
                     GIGAAAAVPESGSPYCELGDLEECAGLALGSPTRFGNMAAPLKHFWDGTSLLWQRGAL
                     AGKPASVFTSTASLHGGQETTLVSMMLPLLHHGMLILGLPYTHAELNATRAGGTPYGA
                     SHFAGIGDDLPIAEHERTLCIAQGRRLAEVAVKLAT"
     gene            1600..2766
                     /locus_tag="VGO02_10945"
     CDS             1600..2766
                     /locus_tag="VGO02_10945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEV7478240.1"
                     /translation="MEKNTWRPLWEFCLAVGRRFFEDRCAQTAGSLSYTTLLSIVPLL
                     TVALALSTAFPVFDQVVGKLQEYILENVLPDTGGLSAFADQINSFTERAGRLTAIGIA
                     GLALTSVLLMMTIDDSLNRIFRVRRRRPLGLRLLTYWAVITLGPLMIGGSLSITSYLV
                     GVSAGYLEMERSVQYPLGILPFLLTCGALALLYIFVPHRHVEWRHGVIGALFAGIAFE
                     LAKRGFAYYIGHFPTYAFIYGTFATILIFLLWLYVSWVVVLVGATLTAMLPGWRNIGA
                     EVNRAPGRELAEALDVLGVLARAQAEGRVMSGAALAREVGILPYRAEAILERGAALGW
                     VARAEKDNWLLARDAHSIKVDDVYRAFVYDAESVGVTDADLELSLHQFTQEKKA"
     gene            2763..>3319
                     /gene="pyrF"
                     /locus_tag="VGO02_10950"
     CDS             2763..>3319
                     /gene="pyrF"
                     /locus_tag="VGO02_10950"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEV7478241.1"
                     /translation="MSAIPRAERLIAALDVADAQQARALVEKLGDAVRFYKVGLELSS
                     TEGYFELIAWLAARGNKVFADLKLYDIPETVRRAVANLRGRGATFLTVHAHRPVMEAA
                     AREKGAMKILAVTVLTSFDQRDLEEMGATKSLGELVLARARGAVETGCDGVIASGLEA
                     TKLKAEFGSRLLVVTPGIRPAEARPA"
BASE COUNT          494 a         1132 c         1176 g          517 t
ORIGIN      
        1 cgcctccgag gacacgacgt cgttgttgtt ctggccgtgg gtgttcagcg agctggcggc
       61 gaccatgaac cccttaccga gccgttcctc ctgcaacacg ctcggcgggc tattctgata
      121 gtggtccgac gcgctgctgc cgccgaaggg ccagacgatc ttgccgttcc atccgcgctg
      181 gggctcccag ggctcccagg gctgcgccgg atcgaacagg accccgatgc gataccagcc
      241 gcgatcgatg acaccggtct cctggcgcac gatgaatggc accacatgcc cctgatcggt
      301 caccgtcgtc gcgacgtcga gcggagggtt cccgggatcg taggcgcgca ggccgctcgc
      361 agccgggctc gtggacttgt agaagaactc gtagcgcgcc gccacgttgc actgtgcgtc
      421 ctgcggcggg ccgaagccgt tcgcctcggt ctcgcagatc cacggctgaa cctggaagcc
      481 cgcgaacacg ggtccgccga tgggatggtt ggtgactgtg atcgatgcgg ccgaagcgcc
      541 ctttgcacgc gccgagagca cgttgctgcc gagagcgagg cctgtgacgc ggcccatgaa
      601 gcggccgttg gcgcgaacgg cgaacgacga cgtcacgtcc cggccgccca cgtccatgcg
      661 cacgctggtc gcgagcacgc ccgcgggcac gacgacctcg gcgagcgcct cgccacccga
      721 gatgaggtcg gcacgattcg acagcacctt gatctcgagc gtgggcgggg cggcgctcgc
      781 gggcgagagc gccggcggca cgacgagtat tgccagcgcg acgaggagcg acacaacgtg
      841 cgtgcgcttc aaactgggca tgaacgcctc cgtaggaccg agccggccag tctgaacgct
      901 cggttccgcg ggagttgtcg ctcaggcgac aagtgactac cgcgatctcg ccggcatata
      961 tgtcgcgcgg atggcggacg acgtgccggt caggtcgcga gcttgaccgc tacctcggcg
     1021 aggcgcctgc cctgcgcgat gcaaagcgtg cgctcgtgct cggcgatcgg caggtcgtcg
     1081 ccgatgccgg cgaagtggct ggcgccgtaa ggcgtgccgc cggcgcgggt ggcgttgagc
     1141 tcggcgtgcg tgtagggcag gccgaggatc agcatgccgt ggtgcaggag cggcagcatc
     1201 atggagacga gggtggtctc ctggccgccg tgcaggctgg ccgtggaggt gaagacgctg
     1261 gccggcttgc cggcgagcgc gccgcgctgc cagaggagcg aggtgccgtc ccagaagtgc
     1321 ttgagcggcg ccgccatgtt gccgaagcgc gtcgggctgc cgagcgcgag gccggcgcat
     1381 tcctcgaggt cgccgagctc gcaatagggc gagcccgact cgggcacggc ggccgcggcg
     1441 ccgatgccgg tggatacgcg gggcaccgtg cgcagccggg cggcggcgcc cggtacgcgc
     1501 tcgatgccct cggcgacgag ctcggccatg cggcgcaccg agccgccgac ggagtagtag
     1561 agaacgagga tgtcggccac ccccggtatc atagaaggaa tggagaagaa tacgtggcgc
     1621 ccgctgtggg agttctgcct cgccgtgggg cggcgcttct tcgaggatcg ctgcgcgcag
     1681 accgccggca gcctgagcta caccacgctg ctctccatcg tcccgctgct caccgttgcg
     1741 ctcgcgctgt cgaccgcctt cccggtgttc gaccaggtgg tcgggaagct gcaggaatac
     1801 atactcgaga acgtcctgcc cgataccggg ggcctgtcgg cattcgccga ccagatcaat
     1861 tccttcaccg agcgcgccgg gcggctgacg gcgatcggca tcgccgggct ggcgctgacc
     1921 agcgtgctgc tgatgatgac catcgacgat tcgctgaacc gcatcttccg cgtgcgccgg
     1981 cgccggccgc tcggcctgcg cctgctcacc tactgggcgg tcatcacgct cggcccgctg
     2041 atgatcggcg gcagcctgtc gatcacctcc tacctggtcg gcgtctcggc cggctacctg
     2101 gagatggagc gcagcgtgca atacccgctc ggcatcctgc cgttcctgct cacctgcggc
     2161 gcgctggcgt tgctctatat cttcgtgccg caccgccacg tcgagtggcg ccacggcgtg
     2221 atcggcgcgc tcttcgccgg catcgcgttc gagctcgcca agcgcggctt cgcctactac
     2281 atcggccact tccccaccta cgcgttcatc tacggcacgt tcgcgaccat cctcatcttc
     2341 ctgctctggc tgtacgtgtc ctgggtggtg gtgctggtcg gcgccacgct caccgcgatg
     2401 ctgcccggct ggcgcaacat cggcgccgaa gtgaaccggg cgccgggacg cgagctggcc
     2461 gaggcgctcg atgtcctcgg cgtgctggcg cgggcgcagg ccgagggcag ggtgatgtcg
     2521 ggggcggccc tggcgaggga ggtcggcata cttccgtacc gcgccgaggc gatcctcgag
     2581 cgcggcgcgg cactcggctg ggtggccagg gcggagaagg acaactggct gctcgcgcgc
     2641 gacgcccact ccatcaaggt cgacgacgtc taccgcgcct tcgtctacga cgcggagagc
     2701 gtcggggtga ccgacgccga cctcgagctt tcgctgcacc agttcacgca ggagaaaaag
     2761 gcatgagcgc aatcccgcgc gccgagcgcc tcatcgcggc gctcgacgtt gccgatgcgc
     2821 agcaggcgcg cgccctggtc gagaagctgg gcgacgcggt gcgcttctac aaggtcggcc
     2881 tcgagctgtc gagcaccgag ggctacttcg agctcatcgc ctggctcgcc gcgcgcggca
     2941 acaaggtgtt cgccgatctc aagctctatg acatcccgga aacggtgcgc cgcgcggtcg
     3001 ccaacctgcg cggccgcggc gccaccttcc ttacggtgca cgctcaccgc ccggtgatgg
     3061 aggctgcggc gcgcgagaag ggcgcgatga agatcctggc ggtgacggtg ctgaccagct
     3121 tcgaccagcg cgacctggag gaaatgggcg ccaccaagag ccttggcgag ctggtgctcg
     3181 cgcgcgcccg cggcgccgtc gagaccggct gcgacggcgt cattgcctca ggcctggagg
     3241 cgaccaagct caaggcagag ttcggcagcc gcctgctggt cgtcaccccc ggcatccgcc
     3301 cggccgaggc tcgcccggc
//
LOCUS       DASYJA010000506         1550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1437297, whole genome shotgun sequence.
ACCESSION   DASYJA010000506 DASYJA010000000
VERSION     DASYJA010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437297"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1550
                     /locus_tag="VGO02_10955"
     CDS             <1..>1550
                     /locus_tag="VGO02_10955"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478242.1"
                     /translation="QGSPQLVYFGTENRPVDPSRWMVPIWAVGGAFFALLALTFVGLG
                     QVMGRAFDAIPNRVAAYSVDVAGSLTGIALFAAMSWLEFSPVAWFAPALALALYFAGW
                     RRPVQLAAAAAALLLVAIGSHFLLVHGRPAWSPYYKVSYDPAQRLISTNDIGHQYIQT
                     VAREGTGYMLPYFLNRGAGGAPFDDVMIIGAGNGNDVSAALQSGAKHVDAVEIDPVIR
                     ELGGRHHPEQPYSDARVAVQVDDGRAFARRTERKYDLAVYALVDSLVLHSGYSSLRLE
                     NFLFTREAIEDVKRTLKPDGLFVMYNFYRQGWVVGRLAKIAEEVFGTQPIVISVPRRD
                     SIRAEDSQAEAITFLIAGDSKRLEAMRARFAKGDILWVSPATAQRQALIAFRPTSPPD
                     GSWAALAPSRVSLEDIGPLPSDDWPQLYLRAREIPWAPIGQGMLVMALLSGLLLYGFA
                     PQRRVRLNPQMFFLGAGFMLLETKGVVHMALLFGSTWIVNSIVFFAILVMILCANLYV
                     LRLQPRRL"
BASE COUNT          205 a          555 c          524 g          266 t
ORIGIN      
        1 agcagggctc gccgcagctc gtctacttcg gaaccgagaa ccgcccggtc gacccgagcc
       61 gctggatggt gccgatctgg gcggtcggcg gcgcgttctt cgcgctgctc gcgctcacct
      121 tcgtcggcct gggccaggtg atggggcgcg cgttcgacgc gatcccgaac cgggtggccg
      181 cctactcggt ggacgtcgcc ggcagcctga ccgggatcgc gctgttcgcg gccatgagct
      241 ggctggaatt ctcgccggtg gcctggttcg cgcccgcgct ggcgctcgcc ctgtacttcg
      301 cggggtggcg ccggccggtg cagctcgccg cggcggccgc cgctctcctg ctagtggcga
      361 tcggctcgca cttcctgctg gtccacggcc ggccggcatg gtcgccctat tacaaggtgt
      421 cctacgaccc ggcgcagcgc ctgatctcca ccaacgacat cggccaccag tacatccaga
      481 cggtcgcgcg cgagggcacc ggatacatgc tgccgtattt cctcaaccgg ggcgccggcg
      541 gcgcgccgtt cgacgatgtc atgatcatcg gcgccggcaa cggcaacgac gtgtcggcgg
      601 cgctgcagtc gggcgcgaag cacgtcgacg cggtcgagat cgacccggtg atccgcgagc
      661 tcggcggccg ccatcacccg gagcagccgt actccgatgc gcgggtcgcg gtgcaggtcg
      721 acgacggccg cgccttcgcg cgccgcaccg agcgcaagta cgacctcgcc gtctacgcgc
      781 tggtcgattc gctggttctg cactcgggct actccagcct gcggctggag aacttcctgt
      841 tcacgcgcga ggccatcgag gacgtgaagc gcaccctcaa gcccgacggc ctgttcgtga
      901 tgtacaactt ctaccgccag ggctgggtgg tcggccggct ggcgaagatc gccgaggagg
      961 tgttcggcac gcagccgatc gtgatctcgg tgccgcgccg cgattcgatc cgcgccgagg
     1021 acagccaggc cgaggccatc acctttctca tcgccggcga ttcgaagcgc ctggaggcga
     1081 tgcgcgctcg attcgccaag ggcgacatcc tgtgggtcag cccggcaact gcgcagaggc
     1141 aggcgctcat cgccttccgc ccgacctcgc cgcccgacgg cagctgggcc gcgctcgcgc
     1201 cctcgcgcgt cagcctcgag gacatcggcc cgctgccgag cgacgactgg ccgcagctct
     1261 acctgcgcgc tcgcgagatc ccctgggcgc cgatcgggca gggcatgctg gtgatggcgc
     1321 tgctctccgg cttgctcctc tacggtttcg cgccgcagcg gcgcgtgcgg ctcaacccgc
     1381 agatgttctt cctcggcgcc ggcttcatgc tgctcgaaac caagggcgtg gtgcacatgg
     1441 cgctgctctt cggcagcacc tggatcgtca actcgatcgt gttcttcgcc atcctggtga
     1501 tgatcctgtg cgccaacctg tacgtgctgc ggctgcagcc gcgacggctt
//
LOCUS       DASYJA010000507         3448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_803520, whole genome shotgun sequence.
ACCESSION   DASYJA010000507 DASYJA010000000
VERSION     DASYJA010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803520"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VGO02_10960"
     CDS             <1..463
                     /locus_tag="VGO02_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV7478243.1"
                     /translation="AQAMPLPTLETVRLLTNLETEKRKLMQVVLFGQPELDTRLASNS
                     VRQIRQRITFQHRMGVLSRNEIADYIGHRLTIAGYSGPTLFSPPAIRAINGASRGVPR
                     LVNILANKSLLLVFGEGLRQVEHHHVRAAVEDTPAAFSRGWWWSRLRSAAD"
     gene            467..1489
                     /locus_tag="VGO02_10965"
     CDS             467..1489
                     /locus_tag="VGO02_10965"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV7478244.1"
                     /translation="MSLINKMLQDLDRRQALGAAGSPAAVRPAPAARAGGREWFWRAL
                     AVLMIASVAWVAWVAYQLMPRPGVATDDAFRAAAQAQKTVAVTAAPQPVPQPQPPPAE
                     TLKLALEIATPVAAPPEKPEAIKPEPVKPARSAKTAEAQGSVSKRDHVRNSLEVADAY
                     FRRAVLLLNQGRVSEAEDQLVAALVAHPAHAQARQAYVSLLLEQRRVDAARRVLQEAL
                     VLNPAQPTFALALARIHAQQREYAAALEVMESAGSVASNADFQALRGVILQRMGRDAE
                     AVEALQNAVRGGAQPGSTWVALGISLEAVARKPEAAQAYRRALDTGPLAAEARAYAES
                     RARALE"
     gene            complement(1496..2188)
                     /locus_tag="VGO02_10970"
     CDS             complement(1496..2188)
                     /locus_tag="VGO02_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV7478245.1"
                     /translation="MRIAILEDDPDQLVLLKRWVGEDGHDVHAYQSGREAMKQAGRES
                     FDLFVLDWQVPDVSGAEVLMWIRANVSKSVPVLFVTVRDAEQDIVFALEHGADDYMVK
                     PVRRQELVARVHALLRRAYPQEEKKQLSFPPFEIDIQRGEIRKEGSRIDLTPKEFELT
                     VALFRNIGRLMSRGHLQEAVWGRSGDLATRTVDTHVSQVRKKLDLRAESGYRVVPIYN
                     YGYRLEKTSPPS"
     gene            2255..3070
                     /locus_tag="VGO02_10975"
     CDS             2255..3070
                     /locus_tag="VGO02_10975"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF009828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HEV7478246.1"
                     /translation="MRVALLGGGTIARLVLEHMRREGIPGVEIAAIAGRQGSTRAAAL
                     AREFAVPLVLGREALLALRPAAVVEAASHDAVREHLVDLLEAGISVVVLSAGALADDE
                     LRRRAEQAAGRSRALFYVPSGGIGGLDALKTACLGGVDEASIQVAKPPAAWKGIPYVE
                     SRGVLLERISAPLTLFEGPAREGVPHFPQNVNIAAVLALAGIGMDRTRLKVVADPALT
                     FNTHTIRVAGRCGRFTLVLENVPSPDNPKTSWLACYSALAALRSLGSPVRYGT"
     gene            3153..3239
                     /locus_tag="VGO02_10980"
     tRNA            3153..3239
                     /locus_tag="VGO02_10980"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3187..3189,aa:Tyr,seq:gta)
     gene            3326..3399
                     /locus_tag="VGO02_10985"
     tRNA            3326..3399
                     /locus_tag="VGO02_10985"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3358..3360,aa:Gly,seq:tcc)
BASE COUNT          533 a         1187 c         1183 g          545 t
ORIGIN      
        1 ggcgcaagcc atgccgctgc cgacgctcga gaccgtgcgg ctgctgacca acctcgagac
       61 cgagaagcgc aagctgatgc aggtggtcct cttcggccag cccgagctcg acacgcggct
      121 cgcctcgaat tcggtgcgcc agatccgcca gcgcatcacc tttcagcacc ggatgggcgt
      181 gctcagccgc aacgagatcg ccgactacat cggtcatcga ctgacgatcg cgggatactc
      241 cgggccgacg ctcttctcgc cgccggctat cagggccatc aacggcgcga gccgcggcgt
      301 gccgcgcctg gtgaacatcc tcgcgaacaa gtcgctgctg ctggtgttcg gcgagggcct
      361 tcgccaggtg gaacatcatc acgtgcgcgc ggccgtggaa gacacgcccg ccgccttttc
      421 ccgcggctgg tggtggagcc ggttgcgctc ggccgccgac taggcgatga gcctgatcaa
      481 caagatgctg caggacctcg accgccgcca ggcgctgggc gcggccggca gcccagccgc
      541 ggtgcggccg gcgccggctg ccagggccgg cggccgcgaa tggttctggc gcgccctcgc
      601 cgtgctgatg attgcaagcg tcgcctgggt ggcgtgggtg gcttaccagc tgatgccgcg
      661 gcccggcgtc gcgaccgacg acgctttccg tgccgcggcg caggcgcaga agacagtcgc
      721 tgtcacggct gcgccgcagc ccgtgccgca gcctcagccg ccgcccgcgg aaacgctcaa
      781 gctggcgctc gaaatcgcaa cgcccgtcgc ggcgccgccg gaaaaaccgg aagccatcaa
      841 gcccgagccg gtgaagccgg ccaggagcgc gaagaccgcc gaggcgcaag gcagcgtcag
      901 caagcgcgat cacgtgcgca acagcctgga ggtcgccgac gcctacttcc ggcgcgcggt
      961 gctgctgctg aaccaggggc gggtcagcga agccgaggat cagctggtcg ccgcgctggt
     1021 ggcccacccg gcgcacgccc aggcgcgcca ggcgtacgtc tcgctgctgc tcgagcagcg
     1081 ccgcgtggac gccgcgcgcc gcgtgctgca ggaggcgctt gtgctcaatc ccgcccagcc
     1141 gaccttcgcg ctcgcgctcg cccgtatcca cgcgcagcag cgcgaatacg cggccgcgct
     1201 ggaagtaatg gagagcgccg gctcggtggc gagcaacgcg gatttccagg cgctgcgcgg
     1261 cgtgatcctg cagcgcatgg gacgcgatgc ggaagccgtg gaggcgctgc aaaacgcggt
     1321 tcgcggcggg gcgcaacccg gctcgacctg ggtcgctctg ggcatctccc tcgaggcggt
     1381 ggcgcgaaaa ccggaggcgg cgcaagccta tcggcgcgcg ctcgacaccg gtcccctggc
     1441 tgcggaagcg cgcgcctacg ccgagagccg ggcgcgcgcg ctggaatagc ccgcctcagc
     1501 tcggcggcga ggttttctcc aggcggtagc cgtagttgta gatcggcacg actcggtagc
     1561 ccgactcggc gcgcaggtcg agcttcttcc tgacctggct tacgtgcgtg tcgaccgtgc
     1621 gcgtggcgag gtcgccgctg cggccccaga ccgcctcctg caggtgtccg cgcgacatca
     1681 ggcggccgat gttgcggaac agggcgaccg tcagctcgaa ctccttgggc gtgaggtcga
     1741 tgcgcgagcc ttccttgcgg atctcgccgc gctggatgtc gatctcgaac ggcgggaagg
     1801 agagctgctt cttctcctcc tgcgggtagg cgcggcgcag aagggcgtgc acccgggcga
     1861 ccagctcctg gcggcgcacc ggcttgacca tgtagtcgtc ggcgccgtgc tcgagcgcga
     1921 agacgatgtc ctgctcggcg tcgcgcacgg tgacgaacag caccggcacg cttttcgaga
     1981 cgttggcgcg gatccacatc agcacctcgg cgccggacac gtcgggcacc tgccagtcga
     2041 gcacgaacag gtcgaagctt tcgcgtccgg cctgcttcat cgcctcgcgc ccgctctggt
     2101 aggcgtgcac gtcgtggccg tcctccccga cccagcgctt gagcagtacc agctggtcgg
     2161 gatcgtcctc gagaatcgca atgcgcacgg tcagtctccc ttgccggtca atgataacgc
     2221 tatgggagaa tcgctgctcc cccaaggaga tgccatgcgc gttgcattgc tcggcggcgg
     2281 caccatcgcg cggctggtgc tggaacacat gcgccgtgaa ggaataccgg gtgtagaaat
     2341 tgccgctatc gcgggccggc agggctcgac tcgcgctgcg gcgctcgccc gggagttcgc
     2401 cgtccccctg gtgctcggac gcgaggcgct gctcgcgctg cggccggctg cggtcgtcga
     2461 ggccgcttcg cacgacgccg tgcgggagca tctggtcgac ttgctcgaag ccggcatcag
     2521 cgttgtcgtt ctatccgccg gcgcgctcgc cgacgacgag ctgcggcggc gtgccgagca
     2581 ggcggcggga cgctcgcggg cgctgttcta cgtcccctcg ggcggcatcg gcggcctgga
     2641 cgccctcaag accgcgtgcc tcggcggcgt ggacgaggcg tcgatccagg tcgccaagcc
     2701 gcccgccgcc tggaagggca ttccctatgt cgaatcccgc ggcgtgctgc tcgagcgcat
     2761 ttccgctccg ctcacgctgt tcgagggccc ggcgcgcgag ggtgtgccgc actttccgca
     2821 aaacgtgaac atcgccgccg tgctggcgct ggccggcatc gggatggacc ggacccgcct
     2881 gaaagtcgtt gcggacccgg cccttacgtt caatacgcac accatccggg tcgccggccg
     2941 ctgcggacgc ttcacgctag tgctggagaa tgtgccctcg ccggacaacc cgaagacctc
     3001 ctggcttgcc tgctacagcg cgctcgccgc cctgcgctct ctcggctcgc cggttcgcta
     3061 cggcacctga tattcggcgc aaaactctgc gaaatcttgt cgccgagtgt tgaccaagcg
     3121 tcggaaaact cacataattt cgggttccgc acggaggggt gcccgagcgg ctaaaggggg
     3181 cagactgtaa atctgttggc cttgcgccta cgtaggttcg aatcctaccc cctccaccag
     3241 cttctccggg cggggcggat accggacccg ggtgaaggac gacgcaagta cgaggaagtg
     3301 atcaaccgtt acgaacgcat ggcaggcggg tgtagctcaa tggcagagca gaagccttcc
     3361 aagcttacga cgagggttcg attcccttca cccgctccag acgaattcgg cccatgtagc
     3421 tcagtggtag agcactccct tggtaagg
//
LOCUS       DASYJA010000508         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3073263, whole genome shotgun sequence.
ACCESSION   DASYJA010000508 DASYJA010000000
VERSION     DASYJA010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3073263"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VGO02_10990"
     CDS             <1..724
                     /locus_tag="VGO02_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV7478247.1"
                     /translation="SGPDINAKGSSRRHVTRAVEASLKRLQTDRIEILYLHQRDALTP
                     IEETMRALEDVVRSGKVLYPALSNHSAWETQAALAAQEANGWARIEAIQPMYSLVKRQ
                     AEVEILPMAAANNVSVFPYSPAGGGLLSGKYADKAAQGRIATNKQYTARYGDAWMHDV
                     ATEFAAFCKKRGFHPMSVAVAWVAAHPAVTAPIIGARSLQQLQASLDSLKIEMTPALW
                     AEIAALSRTPPPATDRLEEQKK"
     gene            complement(774..1826)
                     /locus_tag="VGO02_10995"
     CDS             complement(774..1826)
                     /locus_tag="VGO02_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEV7478248.1"
                     /translation="MKLLVLPGDGIGPEITAATLTVLKAADRLFKLNLSFEEQQVGLA
                     TLKSHGTTMPDGLLGHASEADGIILGPLDQMAYPAREKGGINVSAVFRVKLDLYANIR
                     PARSRPGLGDGKPMDLVIVRECTEGFYADRNMHQGSGEFMPTPDVAMSMRKITAQACE
                     RIARRAFELSLKRKKMVTAVHKANVFRVTDGLFLDKVREVAAEFRGVKLEEILVDAMA
                     ARLVRDPGRFDVVLSTNMYADILSDLASELSGSLGLAGSVLAGDALCCAQAQHGSAPD
                     IAGQDKANPTSLILSAAMLLEWLGEKRKAKGSGKAAEAITAAVDKVLADPKKRTADLG
                     GKVGTRAFGEAVAKAL"
     gene            1852..>2256
                     /locus_tag="VGO02_11000"
     CDS             1852..>2256
                     /locus_tag="VGO02_11000"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEV7478249.1"
                     /translation="MCRALLYLGEPVLLDNFLYRPDSALVRQSYMPKMLNMMNLAGFG
                     LRAWDPASPSPELPYSYYSQSLPVFDRNLKNLAEKIRAGCLLAHVRGVAYNTRVEISV
                     QNCHPFHFKDVPLVMAHNGDLAGFAEMKPLLSS"
BASE COUNT          380 a          798 c          693 g          385 t
ORIGIN      
        1 ctccgggccc gacatcaacg cgaagggcag ctcgcgccgc cacgtcaccc gcgcggtcga
       61 agcgagcctg aagcggctgc agaccgaccg catcgaaatc ctctacctgc accagcgcga
      121 cgcgctgacg ccgatcgagg agacgatgcg cgcgctggag gacgtggtgc gctcgggcaa
      181 ggtcctctat cccgccctca gcaaccattc ggcgtgggag acgcaggcgg cgctcgccgc
      241 gcaggaggcg aacggctggg cgcgcatcga ggcgatccag ccgatgtaca gcctggtgaa
      301 gcgccaggcc gaggtcgaga tcctgccgat ggcggcggcg aacaacgtct cggtcttccc
      361 ctacagccct gcgggcggcg ggctgctttc cggcaagtac gccgacaagg cggcgcaggg
      421 ccgcatcgcc accaacaagc agtacacggc gcgctacggc gacgcctgga tgcacgacgt
      481 ggcgaccgag ttcgccgcgt tctgcaagaa gcgcggcttc cacccgatga gcgtggccgt
      541 ggcctgggtc gcggcgcatc cggcggtaac agctccgata atcggcgcga ggagcctgca
      601 gcaactccag gcctcgctcg actcgctgaa gatcgagatg acgcccgcgc tgtgggccga
      661 gatcgccgcg ctgtcgcgca ccccgccccc ggcgacggac cggctggaag aacaaaagaa
      721 gtagaaatcg gggtcagacc ctgatttttc ttgggagggt ctgaccccga tttctaaagc
      781 gccttggcta ccgcctcgcc gaaagcccgc gtccccacct tcccgccgag atcggcggtg
      841 cgcttcttcg gatcggccag caccttgtcc accgccgccg tgatcgcctc ggccgccttg
      901 ccagacccct tcgcttttct cttttctccc agccactcga gcagcatcgc cgccgagaga
      961 atcagcgaag tcgggttcgc cttgtcctgg ccggcgatgt ccggcgccga gccgtgctgc
     1021 gcctgcgcgc agcacagtgc gtcacccgcc agcaccgatc cggcgagccc caggctgccg
     1081 gaaagctcgg aggcgaggtc cgacaggatg tcggcataca tgttggtcga cagcaccacg
     1141 tcgaagcgtc ccgggtcgcg caccaggcgc gccgccatcg cgtcgaccag gatctcctcc
     1201 agcttcacgc cgcggaactc cgccgccacc tcgcgcacct tgtcgaggaa caggccgtcg
     1261 gtcacgcgga acacgttcgc cttgtgcacc gccgttacca ttttttttct tttaagagag
     1321 agctcaaagg ctcttcgcgc aattctttcg caggcctgcg ccgtgatctt gcgcatcgac
     1381 atcgccacgt cgggcgtcgg catgaactcg ccgctgccct ggtgcatgtt gcggtcggcg
     1441 tagaacccct cggtgcactc gcgcacgatc accaggtcca tcggttttcc gtcacccagt
     1501 cccggccggc tgcgggccgg gcggatattg gcgtagaggt cgagcttcac gcggaacacc
     1561 gccgacacgt tgatcccgcc cttctcgcgc gccggatacg ccatttgatc gagcggcccg
     1621 aggatgatcc cgtccgcctc ggatgcgtgg ccgagcagcc cgtcgggcat cgtcgtgccg
     1681 tggctcttca gcgtggcaag cccgacctgc tgctcttcga aggaaagatt cagcttgaaa
     1741 agacggtcgg cagctttcag gacagtcagg gtggcagcgg taatctccgg cccgatgccg
     1801 tcgccgggta ggacgagcaa tttcatggcg gcgtagggta acctacctcc catgtgccgc
     1861 gcgctcctct acctcggcga gcccgtgctg ctcgacaact tcctctaccg gcccgactcg
     1921 gcgctggtgc gccagtcgta catgccgaag atgctcaaca tgatgaacct cgccggcttc
     1981 ggcctgcgcg cctgggatcc ggcgagcccc agccccgagc tgccctactc ctactactcc
     2041 cagtccctgc cggtcttcga ccgcaacctg aagaacctcg ccgagaaaat ccgcgccggc
     2101 tgcctgcttg cccatgtaag aggcgtggcc tacaacacgc gcgtcgaaat ctccgtgcag
     2161 aactgccatc cgttccactt caaggacgtg cccctggtca tggcgcacaa cggcgacctc
     2221 gcggggtttg ccgagatgaa gcctttgctt tcttct
//
LOCUS       DASYJA010000509         4417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1710486, whole genome shotgun sequence.
ACCESSION   DASYJA010000509 DASYJA010000000
VERSION     DASYJA010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1710486"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VGO02_11005"
     CDS             complement(<1..456)
                     /locus_tag="VGO02_11005"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEV7478250.1"
                     /translation="MTYQENFINGAEEQSLLAAIGDLPLQEASYKQYTAKRRIASYGA
                     TYDFSKNKLQPGPPIPPALFPLREKVAAWVGLPADEFRHALVTEYRAGTALGWHRDTP
                     EFGVVVGVSLANATRMRFRPYPPAKGRDPRAFALMLEPRSAYVMQRDIRW"
     gene            complement(486..1838)
                     /gene="gor"
                     /locus_tag="VGO02_11010"
     CDS             complement(486..1838)
                     /gene="gor"
                     /locus_tag="VGO02_11010"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019247707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEV7478251.1"
                     /translation="MAFDFDLFTIGGGSGGVRASRFAANYGARVAIAESGLFGGTCVN
                     VGCIPKKLLSYAAHFREDFEIAKGFGWTVGEPSFDWNKLLANKDREIARLNAVYERVL
                     TLAKVEIIRGRATVLGPHEIEINGKKHSTKHILVATGSWPVIPNIPGREHAISSNEAF
                     HLERLPRRALVVGGGYIALEFASIFHGLGVQTTLSYRGKRFLRGFDAELGERMAEEMA
                     AKGMTLHFGGEPVAIEKNSNNLKITYSDGSRQDADLVLFATGRKPNSLDLGLEQAGVK
                     LAVDGAVIVDRYSASSVDSIHAIGDVTNRINLTPVATAEAMWLARTLFKGEPTAIDHD
                     NVPTAVFANPNLATVGLSEEKARERFGAVDIYRTSFRSLKLTMTEKKERTFMKLVVER
                     ASQRVVGAHMIGPDAGEVIQGMAIAVKLGATKAQFDATIGIHPTGAEEFVTMREKAPS
                     "
     gene            complement(1839..2156)
                     /locus_tag="VGO02_11015"
     CDS             complement(1839..2156)
                     /locus_tag="VGO02_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478252.1"
                     /translation="MAGATRWYNDFEQEGCAMSMHDKTLKLVTNLDRAAIEARLADVR
                     KAAQAASLADVTGALAGFEGAPRAQMEEKVKNALRALATKPEQKSLSAQLELVEINLP
                     NLK"
     gene            complement(2128..3753)
                     /locus_tag="VGO02_11020"
     CDS             complement(2128..3753)
                     /locus_tag="VGO02_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006164676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV7478253.1"
                     /translation="MAPPLPRPAATLVLLRPGGAGPQVLMIQRVQSAAFLGGAYVFPG
                     GALDASDGDLRFLRRVVGLTDAEASTRLGLPEKGLAYWVAAVRECFEEAGVLLALDAE
                     HRPIEAARAEALARNRKRSFLDLLEKENLFIPAHHIAYYGHWITAPGRSRRFDTRFFV
                     AQAPEGQEGSHDATETVQHVWIRPEDALKRGEKGEIELVPATQTTLRDVGRFDEPRLA
                     FEYARDQPEIEVNRACWAQGKDGPKLFRRADPQYFEIHWTDPQETGQTGYELIAGIPK
                     RLDARVTRLIAPNPGVMTGPGTNTYLVGEQDLAVIDPGPDIDSHVERIVAEGNGRIRW
                     ILCSHTHLDHSPAAAKLKAATGAMLLGRAAPAGQDATFVPDRVVNDGERLYLGGVGLR
                     AIHTPGHASNHLCYLMEDSRMLFTGDHVMQGSTVVINPPDGDMRQYFASLERLLAEDL
                     AVLAPGHGYLIGSPHKELRRLIAHRLAREKKVLAAIGKLRAASLEELLPLVYDDVPAR
                     IHGVAARSLTAHLDKLVADGAARADGGRYALVQ"
     gene            complement(3753..>4417)
                     /locus_tag="VGO02_11025"
     CDS             complement(3753..>4417)
                     /locus_tag="VGO02_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020693804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7478254.1"
                     /translation="VDAMVQRIWDESGPLTGLVNNAAGNFISPTKDLTPNGFNAIANI
                     VFHGTFYVTHAVGKRWIAGGHPGTVLSILVTWVHTGSPYVVPSAMSKAGLHVMTKSLA
                     VEWGRHRIRLNAIAPGPFPTEGASNRLRPGGNFSDSSKSNPMRRVGRMEELQNLATFL
                     MADGCEWLTGETIAVDGGGYLATGSGGYFTELDKLSDADWEKMRAMIKAQNDKDRAGR
                     TT"
BASE COUNT          650 a         1434 c         1562 g          771 t
ORIGIN      
        1 ccagcggata tcgcgctgca tgacgtaggc cgagcgcggc tcgagcatga gtgcgaaggc
       61 gcgcgggtcg cggcctttgg ccggcggata gggacgaaag cgcatgcgcg tggcgttggc
      121 gagcgagacg ccgaccacga cgccgaactc cggcgtgtca cggtgccaac cgagcgcggt
      181 gcctgcgcgg tattcggtga ccagcgcgtg acggaactcg tcggcgggca ggccgaccca
      241 cgcggcgacc ttctccctca agggaaacag ggccggcgga attggcggac cgggctggag
      301 cttgttcttc gagaaatcgt aggttgcgcc atagctcgcg atgcggcgct tggcggtgta
      361 ctgcttgtag gaggcttcct gcagcggcag atcgccgatc gcggccagga gcgactgctc
      421 ttcggctccg ttgatgaaat tttcctggta ggtcaagcca tcaggaaaag cctggaacag
      481 gtccttcagc ttggcgcctt ctcccgcatg gtgacgaact cttccgcccc ggtgggatgg
      541 atgccgatgg tggcgtcgaa ctgcgccttg gtggcgccga gcttcaccgc gatggccatg
      601 ccctggatca cctcgccggc gtccggcccg atcatgtgcg cgccgacgac gcgctggctg
      661 gcgcgctcga cgaccagctt catgaaggtg cgctccttct tttccgtcat ggtcagcttc
      721 aggctgcgga acgaggtcct gtagatgtcg accgcgccga agcgctcgcg cgccttctct
      781 tccgacaggc cgacggtggc gaggttggga ttggcgaaca cggcggtcgg tacgttgtcg
      841 tggtcgatcg cggtgggctc gcccttgaac agggtgcgcg cgagccacat cgcctcggcg
      901 gtcgccaccg gcgtgaggtt gatgcggttg gtgacgtcgc cgatggcgtg gatcgagtcc
      961 accgagctgg ccgagtacct gtcgacgatc acggcgccgt ccacagccag cttgacgccc
     1021 gcctgctcca gcccgaggtc gagggagttc ggcttcctgc cggtggcgaa caacaccagg
     1081 tccgcgtcct gccgcgagcc gtccgaatag gtgattttta gattatttga atttttttct
     1141 attgcgacag gctcgccccc gaaatgcagc gtcatgccct tagccgccat ctcctcggcc
     1201 atgcgctcgc cgagctcggc gtcgaagccg cgcaggaagc gcttgccgcg ataggacagc
     1261 gtggtctgca cgcccaggcc gtggaagatg gaggcgaact ccagcgcgat gtagccgccg
     1321 cccaccacca gcgcgcggcg cggcagccgc tcgaggtgga aggcctcgtt ggagctgatg
     1381 gcgtgctcgc gtcccgggat gttcgggatc accggccagg aaccggttgc caccaggatg
     1441 tgcttggtgg agtgcttttt cccgttgatt tctatttcat gaggaccaag aaccgttgcc
     1501 ctgccgcgga tgatctcgac cttggcgagc gtgaggacgc gctcgtagac ggcgttcagg
     1561 cgcgcgatct cgcggtcctt gttggcgagc agcttgttcc agtcgaagct aggttcgccg
     1621 acagtccagc cgaacccctt ggcgatctcg aagtcctcgc ggaaatgcgc ggcgtaggag
     1681 aggagcttct tcgggatgca gccgacgttg acgcaggtgc cgccgaacag gcccgactcg
     1741 gcgatggcga cgcgcgcgcc gtagttcgcg gcgaagcggc tcgcgcgtac gccgccggag
     1801 ccgccgccta tcgtgaaaag atcaaaatcg aaagccaact acttgagatt ggggaggttg
     1861 atctccacca gctcgagttg cgcggagagg ctcttctgct cgggcttggt ggcgagcgcc
     1921 ctgagcgcgt tcttcacctt ctcctccatc tgcgcgcgcg gcgcaccctc gaagccggcc
     1981 agggcgccgg tgacgtcggc gaggctggcg gcctgcgccg ccttgcgcac atcggcgagc
     2041 cgcgcttcga tcgcggcacg gtccaggttg gtcaccagct tgagagtctt gtcgtgcatg
     2101 ctcatcgcgc agccctcctg ctcgaaatca ttgtaccagc gcgtagcgcc cgccatcggc
     2161 gcgcgccgcc ccgtcggcca ccagcttgtc gaggtgcgcc gtgagcgagc gcgccgccac
     2221 gccgtggatg cgcgccggca cgtcgtcgta gaccagcggc agcagctcct cgagcgaggc
     2281 cgcgcgcagc ttgccgatgg cggccagcac cttcttttcg cgggccaggc ggtgggcgat
     2341 caggcggcgc agctccttgt gaggagagcc gatcaggtag ccgtggccgg gcgcgaggac
     2401 cgccaggtcc tcggcgagca ggcgctcgag cgaggcaaag tactggcgca tgtcgccgtc
     2461 gggcgggttg atgaccaccg tggagccctg catcacgtgg tcgccggtga acagcatgcg
     2521 gctgtcttcc atcaggtagc agaggtggtt cgaggcgtgc ccgggcgtgt ggatggcgcg
     2581 caggccgacg ccgcccaggt acaggcgctc gccgtcgttc accacgcggt ccggcacgaa
     2641 ggtagcgtcc tggcctgcgg gcgccgcgcg ccccagcagc atcgctccgg tggcggcctt
     2701 gagcttcgca gccgccgggg aatggtccag gtgcgtgtgg ctgcacagga tccagcggat
     2761 ccggccgttt ccctcggcga cgattctttc aacgtgggaa tcgatgtccg gacccgggtc
     2821 gatcaccgcc aggtcctgct cgcccaccag gtaggtgttg gtgcccggcc cggtcatcac
     2881 gccgggattg ggcgcgatga ggcgcgtcac gcgggcatcg aggcgcttgg gtatgcctgc
     2941 gatcagctca tagccggttt gcccggtctc ctgcgggtcg gtccagtgga tctcgaagta
     3001 ctgcgggtcg gcgcggcgga acagcttcgg cccgtccttg ccctgcgccc agcaggcgcg
     3061 gttcacctcg atctcgggct ggtcgcgcgc gtactcgaag gcgaggcgcg gctcgtcgaa
     3121 gcgccccacg tcgcgcagcg tggtctgcgt ggcgggcacc agctcgatct cgcccttctc
     3181 gccgcgcttg agcgcgtcct ccggccggat ccagacgtgc tgcaccgtct cggtggcgtc
     3241 gtgcgagccc tcctggccct cgggcgcctg ggcgacgaag aagcgcgtgt cgaagcgccg
     3301 cgagcgcccg ggcgccgtga tccagtggcc gtagtaggcg atgtggtgcg cggggatgaa
     3361 caggttctct ttttccagca ggtcgaggaa cgagcgcttg cggttgcgcg ccagcgcttc
     3421 ggcgcgcgcc gcctcgatcg gccggtgctc ggcgtcgagc gcgagcagca cgccggcttc
     3481 ctcgaagcac tcgcgcaccg cggccaccca gtaggcgagg cccttctccg gcaggccgag
     3541 gcgcgtgctc gcctcggcgt cggtcagccc gacgacgcgg cgcaggaagc gcaggtcgcc
     3601 gtccgacgcg tcgagcgcgc cgccgggaaa gacgtaggcg ccgccgagga aggccgcgct
     3661 ctgcacgcgc tggatcatca gcacctgcgg accggcgccg ccgggacgca gcagcaccag
     3721 cgtggccgcc gggcggggaa ggggtggtgc cattacgtgg tgcggccggc gcggtctttg
     3781 tcgttttgcg ccttgatcat cgcgcgcatt ttttcccaat cggcgtcgga aagcttgtcg
     3841 agctccgtga agtagccgcc cgagccggtg gcgaggtagc cgccgccgtc gacggcgatg
     3901 gtctcgccgg tgagccactc gcaaccgtcg gccatcagga acgtcgccag gttctgcagc
     3961 tcctccattc tgcccacgcg acgcatggga ttggatttgc tcgagtcaga aaaatttccc
     4021 ccaggacgaa gcctattgga ggccccctcg gtcgggaacg ggccgggcgc gatcgcgttg
     4081 aggcggatgc ggtgccggcc ccattcgacc gccagcgatt tcgtcatcac gtgcagcccg
     4141 gccttcgaca tcgccgaggg cacgacgtag ggcgagccgg tgtgcaccca ggtgacgagg
     4201 atcgagagca cggttccggg atggccgccg gcgatccagc gcttgcccac ggcgtgggtg
     4261 acgtagaacg tgccgtggaa cacgatgttg gcgatggcgt tgaagccgtt gggcgtcagg
     4321 tccttcgtcg ggctgatgaa attgccggcg gcattgttga ccaggccggt gagcggcccg
     4381 ctctcgtccc agatgcgctg caccatcgcg tcgaccg
//
LOCUS       DASYJA010000510         3402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1894096, whole genome shotgun sequence.
ACCESSION   DASYJA010000510 DASYJA010000000
VERSION     DASYJA010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1894096"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VGO02_11030"
     CDS             <1..516
                     /locus_tag="VGO02_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEV7478255.1"
                     /translation="GKSTLFNALAHATAYSADQLFATLDTTTRRVWIPEAGNVVLSDT
                     VGFIRDLPHGLVAAFRATFEETVQADLLLHVVDAASPVRDAQAEQVDKVLDEIPAGEA
                     PRLLVWNKIDAAGLEPAVERDQYGRICRVFVSARAGAGLDGLRGAIAEFARERAAARS
                     ATAPEQSLQAH"
     gene            521..1726
                     /gene="hflK"
                     /locus_tag="VGO02_11035"
     CDS             521..1726
                     /gene="hflK"
                     /locus_tag="VGO02_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEV7478256.1"
                     /translation="MSLNDPNWGRGSRGPQGPGGNQGPPDLDELWRNFNRRLSELFGR
                     RKRDGDEPPSGGGGPSPWQFGGGAGVLVALVLAVWLASGFYIVVEGQRGVVLTFGRFS
                     QETSPGLRWRMPWPIQSAEIVNLAQVRTLEVGYRNNVRTKVLKESLMLTDDENIVDLQ
                     FAVQYLVKDAKDFVFNVRRPDDSAMQIAETAMREVIGKNRMDAILYETQVDIANKSRD
                     LMQEIHDRYATGITISTVTIQNAQPPEQVQAAFDDAVKAGQDRERQKNEGQAYANDVI
                     PRARGTASRLLQEGEGYKQRVIATAEGDASRFRQVLTEYAKAPAVTRERIYIETMQQV
                     LTATSKIMMDYRGSGNLLYLPLEKLMQGSAAPGGDAATAPRPATPEPAPDAGARSRET
                     LRNRDRGER"
     gene            1726..2610
                     /gene="hflC"
                     /locus_tag="VGO02_11040"
     CDS             1726..2610
                     /gene="hflC"
                     /locus_tag="VGO02_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEV7478257.1"
                     /translation="MKTLGPILALFIVIALLASSTLYSVDQRQNAIVFQLGEVKDVVK
                     APGLHFKWPLLQNVRLFDMRILTFDDAEPLRFLTQGNRPVLVDSFVKWRVADVKQYYV
                     SVQGDEFRAQTRIKQTVSGTLRDEFGVRGLHEVVSGEREQIMSRVREKVDQDLERIGV
                     KIVDVRLKRVDLPPDVSESVYRRMEAERKRIANELRSTGAAEAEKIRADAERQREVIL
                     AEAYRDAQRVRGEGDGKSAAIYAAAFNQNPEFYSFYRSMEAYRSSFRGRNDVMLLEPN
                     SDFLRYFRDSLGKGAARK"
     gene            2631..2819
                     /locus_tag="VGO02_11045"
     CDS             2631..2819
                     /locus_tag="VGO02_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HEV7478258.1"
                     /translation="MGTTLLLALALMLILEGVLPFLAPNLWRDTFRRITQMSDGQIRF
                     VGLSSMIVGLLLLLWART"
     gene            2816..>3402
                     /locus_tag="VGO02_11050"
     CDS             2816..>3402
                     /locus_tag="VGO02_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEV7478259.1"
                     /translation="MKWLLPEYVEDILPAEAMRIEALRRAILDLFFAQKYEFVIPPML
                     EYMDSLLTGTGHDLELRTFKVVDQLSGRMMGLRADITPQVARIDAHLLNRKGVTRLCY
                     CGAVLHTRPAGPAATREPIQIGAEMFGEPGVAGDLEVQRLLCEALALAGAKSVRLDVG
                     HVAVFRSIVHAASLDNERESELFEALRRAILDLFFA"
BASE COUNT          566 a         1161 c         1166 g          509 t
ORIGIN      
        1 ggcaagtcga cgctgttcaa cgcgctggcg cacgccacgg cctactcggc ggaccagctg
       61 ttcgcgaccc tcgatacgac cacgcggcgg gtgtggattc ccgaggcggg caacgtcgtg
      121 ctgtcggata ccgtcggctt catccgcgat ctgccgcacg gcctggtggc ggcgttccgt
      181 gccactttcg aggagacggt gcaagccgat ctgctgctgc acgtggtcga tgccgcctcg
      241 ccggtgcgcg acgcgcaggc cgagcaggtc gacaaggtgc tggacgagat tcccgccggc
      301 gaggcgccgc gcctgctggt ctggaacaag attgacgcgg cggggctcga accggcggtc
      361 gagcgggacc aatatggtag gatttgccgc gttttcgtga gcgcgcgcgc aggcgccgga
      421 ctggatggtc tgcgcggcgc gatcgccgaa ttcgcccggg aaagggcggc ggcgcgaagc
      481 gcgacagctc ccgaacaatc actgcaagct cactgattcc atgtccttga acgaccccaa
      541 ctgggggcgc ggttcccgcg gtccgcaagg tcccggcggc aaccagggcc cgcccgacct
      601 cgacgagctg tggcgcaact tcaaccggcg cctgtcggag ttatttgggc gccgcaagcg
      661 cgacggcgac gagccgcctt cgggcggcgg cggcccctcg ccctggcaat tcggcggcgg
      721 cgccggcgtg ctggtcgccc tggtgctcgc ggtgtggctg gcgagcggct tctacatcgt
      781 ggtcgagggc caacgcggcg tggtgctcac tttcggccgc ttctcccagg aaacatcgcc
      841 ggggctgcgc tggcgcatgc cgtggccgat ccagtcggcc gagatcgtca acctggcgca
      901 ggtgcgcacg ctggaagtcg gctaccgcaa caacgtgcgc accaaggtgc tgaaggaatc
      961 gctgatgctc accgacgacg agaacatcgt cgacctgcag ttcgcggtgc agtacctggt
     1021 gaaggatgcc aaggatttcg tcttcaacgt gcgccggccc gacgactcgg cgatgcagat
     1081 cgccgagacc gcgatgcgcg aagtgatcgg caagaaccgt atggacgcga tcctgtacga
     1141 gacgcaggtc gacatcgcca acaagtcgcg cgacctgatg caggagatcc acgaccgcta
     1201 cgccaccggc atcaccatca gcaccgtgac catccagaac gcgcagccgc ccgagcaggt
     1261 gcaggcggcg ttcgacgacg cggtcaaggc cggccaggac cgcgagcggc agaagaacga
     1321 gggccaggcc tacgccaacg acgtcatccc gcgggcgcgc ggcaccgcga gccgcctgct
     1381 gcaggagggc gagggctaca agcagcgcgt gatcgccacc gccgagggcg acgcctcgcg
     1441 cttccgccag gtgctgaccg agtacgccaa ggcgcccgcc gtgacgcgcg agcgcatcta
     1501 catcgagacc atgcagcagg tcctcaccgc gaccagcaag atcatgatgg actaccgcgg
     1561 ctcggggaac ctgctctacc tgccgctcga gaagctgatg cagggctcgg ccgcccccgg
     1621 cggcgacgcc gcgaccgcgc cgcgcccggc cacgcccgag ccggcgcccg acgcaggggc
     1681 gcgctcgcgc gagacgctgc gcaaccgcga ccggggcgag cgctgatgaa gaccctgggc
     1741 ccgatcctcg ccctgttcat cgtgatcgcg ctgctcgcct cgagcacgct gtacagcgtc
     1801 gaccagcggc agaacgccat cgtcttccag ctgggcgagg tgaaggacgt ggtgaaggct
     1861 cccggcctgc acttcaagtg gccgctgctg cagaacgtgc gcctgttcga catgcgcatc
     1921 ctcaccttcg acgacgccga gccgctgcgc ttcctcaccc agggcaaccg gccggtgctg
     1981 gtcgactcct tcgtcaagtg gcgcgtcgcc gacgtgaagc agtactacgt cagcgtgcag
     2041 ggcgacgagt tccgtgcgca gacgcgcatc aagcagaccg tctcgggcac gctgcgcgac
     2101 gagttcggcg tgcgcggcct gcacgaggtg gtgtcgggcg agcgcgagca gatcatgagc
     2161 cgcgtgcgcg agaaggtcga ccaggacctc gagcgcatcg gcgtcaagat cgtcgacgtg
     2221 cggctgaagc gcgtcgacct gccgcccgat gtcagtgaat cggtgtaccg ccgcatggag
     2281 gcggagagga agcgcatcgc caacgagctg cgctcgaccg gcgccgccga ggccgagaag
     2341 atccgcgccg acgccgagcg ccagcgcgag gtgatcctcg ccgaggccta ccgcgacgcg
     2401 cagcgcgtgc gcggcgaagg cgacggcaag tcggccgcca tctacgccgc ggccttcaac
     2461 cagaaccccg agttctactc gttctaccgc agcatggagg cgtaccgttc gagcttccgc
     2521 ggccgcaacg acgtcatgct gctcgagccg aactccgatt tcctgcgcta cttcagggac
     2581 tccctcggca agggggctgc caggaaatga accggcccga cagatgaggc atgggcacta
     2641 ctctcctcct ggcgctcgcc ctcatgctca tcctcgaggg ggtactaccc ttcctcgcgc
     2701 ctaacctctg gcgcgacacc ttccgccgca tcacgcagat gagcgacggg caaatccgct
     2761 tcgtcggcct gtcttcgatg atcgtcgggc tgctgctgct gctgtgggcc cggacatgaa
     2821 gtggctcctg ccggagtacg tagaggacat cctgccggcc gaggcgatgc gcatcgaggc
     2881 gctgcgccgg gcgatcctgg atctgttctt cgcgcaaaaa tatgaattcg tcatcccgcc
     2941 gatgctggag tacatggatt cgctgctcac cggcaccggc cacgacctgg agctgcgcac
     3001 cttcaaggtg gtcgaccagc tctcgggccg catgatgggc ctgcgcgccg acatcacgcc
     3061 gcaggtggcg cgcatcgacg cgcacctgct caaccgcaag ggcgtgacgc ggctgtgcta
     3121 ttgcggagca gtgctgcaca cgcggccggc gggcccggcg gcgacgcgcg agccgatcca
     3181 gatcggcgcc gagatgttcg gcgagccggg cgtcgccggc gacctcgagg tgcagcgcct
     3241 gctgtgcgag gcgctggcgc tcgccggcgc gaagagcgtg cgcctcgacg tcgggcacgt
     3301 agcggtgttc cgctccatcg tgcatgccgc gagtctcgat aacgagcgcg agtcggaact
     3361 gttcgaggcg ctgcgccggg cgatcctgga tctgttcttc gc
//
LOCUS       DASYJA010000511         2931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1437957, whole genome shotgun sequence.
ACCESSION   DASYJA010000511 DASYJA010000000
VERSION     DASYJA010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437957"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..988)
                     /locus_tag="VGO02_11055"
     CDS             complement(26..988)
                     /locus_tag="VGO02_11055"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7478260.1"
                     /translation="MKVLFAAVSFFLATVAVAQPYPSRAVKVIVPWPPGQATDIAARM
                     VAHKLQEQTGQPFVIDNRPGAGGSLGTAVAATSPADGYTLLAASSGPISIMPSLQKLT
                     YEPQKDFVLISLITRNPYALVVSPSFPASNAKEFVALLKANPDKYTFGSSGTGATAHL
                     LTELFNSMAQVQARHIPYKGSAPALTDVMNGQVSYMIETVAATVGHVRSGRLKALGVS
                     TLRRAAAMPEVPTLAEAADLPDYDAAAWIGYAAPAGIPREALVRLSAEIRKALESGDM
                     KEKLLNAGLDPVASTPEELPAFMRKEQERYAAVIRNANIKLEQP"
     gene            complement(1016..1795)
                     /locus_tag="VGO02_11060"
     CDS             complement(1016..1795)
                     /locus_tag="VGO02_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7478261.1"
                     /translation="MDLGIRGKTALVCAASKGLGKGCAASLAREGVNLVITARGKEAL
                     EATAAEIRSLYKIKVEAIAGDITTPEGRAAALKACPSPDILVNNAGGPPPGDFRNWSR
                     EDWVKALDANMLAPIELIKAVLDGMIARRFGRIVNITSGAVKNPIPELGLSNGARTGL
                     TGFVAGLSRQTVRHNVTINGLLPGPFDTDRLRSNFEFNAKKLGKTAAELAKTRAEANP
                     AGRFGTIEEFGDACAYLCSAQAGFVTGQNFLMDGGAYPGTF"
     gene            complement(1797..2696)
                     /locus_tag="VGO02_11065"
     CDS             complement(1797..2696)
                     /locus_tag="VGO02_11065"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV7478262.1"
                     /translation="MSAASYRSWGVAFAVIGVLAFSLRPILIKLSYAAHPVSPNTLLF
                     LRMALSLPFFLVIAWWLRNQRPALTRRDWAAVAGLGFLGYYAASFLDFLGLQYVGAGV
                     GRLILFLYPTMVLSLAFLFFKKPPTRREIVSLAMSYAGIALVVSDQIGTSGEGKLFLL
                     GVLLVFASALCYAVYLVAGSQVVKRIGSMRFTAYSMAVATAPALVQVFALEPAGALEL
                     PANVWAYAIILATVSTVLPLFLQAEALRRIGATEFALVGAVSPVSVAVMSALGLGEPF
                     GPRQMGGAALVIGGVLLVSLKKG"
     gene            complement(2693..>2931)
                     /locus_tag="VGO02_11070"
     CDS             complement(2693..>2931)
                     /locus_tag="VGO02_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEV7478263.1"
                     /translation="KLAGKAATAEKAKPAEAAAPNPVSDILFGRTGPRGGRQTQGLLE
                     AMAKSAARTVGSSVGREIVRGVLGSILGAGKRRR"
BASE COUNT          476 a          972 c         1024 g          459 t
ORIGIN      
        1 gcggcgagga gggcgatgag gcggctcatg gttgctccag cttgatgttt gcgttgcgga
       61 tgacggcggc gtagcgctcc tgctccttgc gcatgaacgc cggcagctct tccggcgtgc
      121 tcgccaccgg gtcgaggccg gcgttgagga gcttttcctt catgtcgccg gactcgaggg
      181 ccttgcggat ctcggcggac aggcgcacca gggcttcgcg cgggatgccg gccggcgccg
      241 cgtagccgat ccacgccgcg gcgtcgtagt cgggcaggtc ggccgcctcg gcgagcgtcg
      301 gcacctcggg catcgcggcg gcgcggcgca gcgtcgaaac gccgagcgcc ttcaggcgac
      361 ccgagcgcac atggcccacg gtggcggcga cggtctcgat catgtagctc acctggccgt
      421 tcatgacgtc ggtgagggcg ggagcgctgc ctttgtaggg aatatggcgc gcctgcacct
      481 gagccatcga gttgaaaagt tcagtcagca ggtgcgcggt ggcgccggta cccgacgagc
      541 cgaaggtgta cttgtcggga ttcgccttga gcagcgcgac gaactccttg gcattgctcg
      601 ccggaaacga cgggctcacc acgagcgcgt agggattgcg cgtgatcagg ctgatcagga
      661 cgaaatcctt ctgcggctcg taggtcagct tctgcagcga cggcatgatc gagatcgggc
      721 cgctcgacgc ggcgagcagc gtgtagccgt cagcggggct ggtcgcggcg accgccgtgc
      781 cgagcgagcc gcccgcgccg ggccggttgt cgatgacgaa cggctggccg gtctgctcct
      841 gcagcttgtg cgccaccatg cgcgccgcga tgtcggtggc ctggccgggc ggccacggca
      901 cgatcacctt gaccgcccgg ctgggatagg gttgcgcgac ggctacggtc gcgagaaaaa
      961 atgagactgc agcgaacagc actttcatcg atcctcctcc aaggatcccg gcattctaga
     1021 acgtgcccgg atacgcgcca ccgtccatga ggaaattctg tccggtgacg aagccggcct
     1081 gggcgctgca caaataggcg caggcgtcgc cgaactcctc aatggtgccg aagcggccgg
     1141 cgggattcgc ctccgcccgc gtcttcgcca gctcggcagc cgtcttgccg agcttcttcg
     1201 cattgaattc gaaattcgag cgcagccggt cggtgtcgaa ggggccgggc agcaggccgt
     1261 tgatggtgac gttgtggcgc accgtctggc gcgacagccc ggcgacgaag ccggtcaggc
     1321 cggtgcgcgc gccgttggac aggcccagct ccgggatcgg gttcttcacc gcgccggagg
     1381 tgatgttgac gatgcgcccg aagcgccgcg cgatcatgcc gtcgagcacc gccttgatca
     1441 gctcgatcgg cgcgagcatg ttggcgtcga gcgccttcac ccagtcctcg cgcgaccagt
     1501 tgcggaagtc gcccggcggc gggccgccgg cgttgttgac caggatgtcg ggcgacgggc
     1561 acgccttgag cgcggcggcg cggccttcgg gcgttgtgat gtcgcccgca attgcttcta
     1621 cttttatctt gtaaagagaa cgaatttcag ccgcggtggc ctccagcgct tccttgccgc
     1681 gcgccgtgat gacgaggttg acgccttcgc gcgcgagcga cgccgcgcag cctttgccta
     1741 atcccttcga tgccgcgcac acgagcgcgg tcttgccgcg aatgccaaga tccatatcag
     1801 cctttcttga gagaaacgag gagcacgccg ccgatcacca acgccgcgcc gcccatctgc
     1861 cgcgggccga acggctcgcc caggccgagc gcgctcatta ccgccacgct taccgggctg
     1921 acggcgccga ccagcgcgaa ctcggtcgcg ccgatgcgcc gcagcgcctc ggcctgcagg
     1981 aagagcggca gcaccgtgct gaccgtggcg agaatgatcg cgtaagccca gacgttggcc
     2041 ggcagctcga gggcgccggc cggctcgagc gcgaagacct gcaccagcgc cggcgcagtc
     2101 gcgaccgcca tgctgtaggc ggtgaagcgc atggagccga tgcgcttgac cacctggctg
     2161 ccggccacca ggtaaaccgc gtagcacagc gcggaggcga agacgagcag cacgccgagc
     2221 aggaagagct tgccctcgcc gctcgtgccg atctggtccg acacgaccag cgcgatgccg
     2281 gcgtagctca tcgccagcga gacgatctcg cggcgcgtcg gcggtttctt gaaaaacagg
     2341 aacgccagcg acagcaccat cgtcgggtag aggaagagga tcagccggcc gacgcccgcg
     2401 ccgacgtatt gcaatcccag gaagtcgagg aagctcgcgg cgtagtagcc gaggaagccc
     2461 aggccggcga ccgccgccca gtcccggcgc gtgagcgccg gccgctggtt gcgcagccac
     2521 catgctatga cgaggaagaa gggtagcgac agcgccatgc gcaggaagag cagcgtgttc
     2581 gggctgaccg ggtgcgctgc ataggacagc ttgatcagga tcggccgcag cgagaacgcc
     2641 agcacgccga tcacggcgaa agccactccc cagctgcggt acgacgcggc gctcaacggc
     2701 ggcgcttgcc ggcgccgagg atcgagccga ggacgccgcg cacgatttcg cgaccgaccg
     2761 aggacccgac ggtgcgcgcc gcgctcttgg ccatcgcctc gagcaggccc tgcgtttgcc
     2821 tgccgccccg cgggccggtc ctgccgaaca agatgtcgga gaccggattc ggcgctgcgg
     2881 cttccgcggg tttggctttt tcagccgttg ctgcctttcc cgccagcttc t
//
LOCUS       DASYJA010000512         2262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_439424, whole genome shotgun sequence.
ACCESSION   DASYJA010000512 DASYJA010000000
VERSION     DASYJA010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439424"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="VGO02_11075"
     CDS             <1..1180
                     /locus_tag="VGO02_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEV7478264.1"
                     /translation="AHRGGRRPGQGEGLIRAFLLVLGCTALAVQAQPRREDLESLRTR
                     IEALQQELERKEGTRREARDALRDSERAISQANRTLAGIEAESRHLRAESTRNAERRR
                     ALERLAAERESTIGRMLAAQHAAGAPDALRVALSGEDPSALARNLYYLGYVSRAAAAL
                     LREYRAGIAEAARLAAEAEARRGRLRTLELAHRSDRERIVRERRERQRVFDRLAGDIR
                     KSRRDIKQLQADESRLARLVQEIGRVVYGRVEQTPEAGARREAFASLRGKLRLPVRGE
                     LTGRFGAPRGAVGTEAKGVFIRAPQGQPVKAVAYGQVVYADWMRGFGNLLIVDHGQSY
                     LSIYANNESLLKQVGENVTAGETLATTGASGGNEETGLYFELRHLGRAFDPLRWVKLK
                     "
     gene            1204..>2262
                     /locus_tag="VGO02_11080"
     CDS             1204..>2262
                     /locus_tag="VGO02_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEV7478265.1"
                     /translation="MQSKLRNIGLIALGAVAGVLISLNFQAIAERSTRSTLPIEELRA
                     FTEVFGAIKTNYVEPVEDKKLITEAINGMLTGLDPHSAYLDQEAFRDLQVGTQGQFGG
                     LGIEVGMEDGFVKVISPIEDTPAFKAGIKPGDLIIKLDDTPVKGMSLNDAVKKMRGKP
                     NTSIILTISRKGESAPIVVTLTRAVIRVQSVKSKVIEPGYAWIRVSQFQESTPESLVK
                     HLDGLFKQGPVKGLVLDLRNDPGGLLHGAVAISSAFLPPKALVVSTDGRAEDARKKFI
                     ASPEDYARGSRSEDVLKALPAAVKSVPMVVLVNGGSASASEIVAGALQDHKRAIIIGT
                     QTFGKGSVQTIMPLGNNTA"
BASE COUNT          389 a          780 c          782 g          311 t
ORIGIN      
        1 ggcgcatcgc ggcggtcgcc gcccagggca aggcgaaggc ctgatccgcg cgtttcttct
       61 ggtcctcggc tgcacggcgc tcgccgttca ggcgcagccg cggcgggaag acctggagtc
      121 gctgcgcacg cgcatcgagg cgctgcagca ggagctcgag cgcaaggaag gcacccggcg
      181 cgaggcgcgc gacgccctgc gcgactccga gcgcgccatc tcgcaggcca accgcacgct
      241 ggccggcatc gaggccgaga gccggcacct gcgcgccgag agcacgcgca acgcggagcg
      301 gcgccgcgcc ctcgagcggc tggcagccga gcgcgagtcg acgatcggcc gcatgctggc
      361 ggcacagcac gcagcgggcg cgcccgacgc gttgcgcgtg gcgctttccg gcgaggatcc
      421 ttcggccctc gcgcgcaatc tctactacct tggctacgtt tcgcgcgccg cggccgcgct
      481 gctgcgcgag tatcgcgcgg gcatcgcgga ggccgcgcgc ctcgccgccg aggcggaggc
      541 acgccggggg cgtctgcgca cgctcgagct ggcgcatcgc agcgaccgcg agcgcatcgt
      601 ccgggagcgg cgcgagcgcc agcgcgtgtt cgaccgcctc gccggggata tccgcaaaag
      661 ccgcagggac atcaagcagc tgcaggccga cgagagccgc ctggcgcgcc tggtgcagga
      721 gatcgggcgc gtcgtctacg gccgggtcga gcagaccccc gaggccggcg cccggcgcga
      781 ggccttcgcc tcgctgcgcg gcaagctgcg cctgccggtg cgcggggaac tgaccggccg
      841 gttcggcgct ccaaggggtg cggtggggac agaagcgaag ggcgtcttca tccgcgcccc
      901 ccaggggcag ccggtgaagg cggtcgccta cgggcaggtg gtgtacgccg actggatgcg
      961 cggcttcggc aacctgctga tcgtcgacca cggccagtcg tacctgtcga tctacgcgaa
     1021 caacgaatcg ctgctcaagc aggtggggga aaatgtcacg gccggcgaga cacttgcgac
     1081 caccggagca agcggtggca acgaggaaac gggcttatac tttgaattgc ggcacttggg
     1141 ccgcgccttt gacccgctgc gctgggtcaa attgaaataa gcggacggaa agaagcgacc
     1201 cctatgcaaa gcaagctccg caacatcgga ttgatcgccc ttggcgccgt cgccggcgtg
     1261 ctgatcagcc tcaacttcca ggccatcgcc gagcgctcga cgcgctcgac gctgccgatc
     1321 gaggaactgc gcgccttcac cgaggtgttc ggcgccatca agaccaacta cgtcgagccg
     1381 gtcgaggaca agaagctgat caccgaggcg atcaacggca tgctgaccgg ccttgatccc
     1441 cactccgcct acctcgacca ggaagccttc cgcgacctgc aggtcggcac ccagggtcag
     1501 ttcggcggcc tgggcatcga agtcggcatg gaagacggct tcgtcaaggt gatctcgccg
     1561 atcgaggaca cgccggcgtt caaggccggc atcaagccgg gcgacttgat catcaagctc
     1621 gacgacacgc cggtgaaggg catgtcgctc aacgacgccg tgaagaagat gcgcggcaag
     1681 ccgaacacca gcatcatcct caccatctcg cgcaagggcg agagtgcgcc gatagtggtg
     1741 acgctgacgc gcgccgtcat ccgggtgcag agcgtcaagt ccaaggtgat cgagccgggc
     1801 tatgcctgga ttcgcgtctc gcaattccag gagtcgaccc ccgagagcct ggtcaagcac
     1861 ctcgacggcc tgttcaagca aggcccggtc aagggcctgg tgctcgacct gcgcaacgac
     1921 ccgggcggcc tgctgcacgg cgcggtcgcc atctcgtcgg ctttcctgcc tcccaaagcg
     1981 ctggtcgtct cgaccgacgg ccgcgccgag gacgcgcgca agaagttcat cgccagtccc
     2041 gaggactatg cccgcggcag ccgctccgag gacgtcctga aggcgctgcc cgccgcggtg
     2101 aagtcggtgc cgatggtggt cctggtgaac ggcggctccg cctcggcctc cgagatcgtc
     2161 gccggcgccc tgcaggatca caagcgcgcc atcatcatcg gcacgcagac cttcggcaag
     2221 ggctcggtgc agaccatcat gccgctgggc aacaacaccg cg
//
LOCUS       DASYJA010000513         4360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_532431, whole genome shotgun sequence.
ACCESSION   DASYJA010000513 DASYJA010000000
VERSION     DASYJA010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532431"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VGO02_11085"
     CDS             complement(<1..619)
                     /locus_tag="VGO02_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEV7478266.1"
                     /translation="MKSYRIASIPGDGIGNEVIPAGVDVLAALASKEGFELKFESFDW
                     SSARYKKTGAYIPDGGLDRLKTFDAILFGAVGAPDVPDHVSLWGLRLPICQGFDQYAN
                     VRPSRVLPGVSSVLKNAHEIDWVIIRENTEGEYSGAGGRVHRGLENEVAMEVSTFTRS
                     GVERIHKFAFELARKRPRKHLTLVTKSNAQRHGMVLWDEVFFEIHK"
     gene            650..1090
                     /locus_tag="VGO02_11090"
     CDS             650..1090
                     /locus_tag="VGO02_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV7478267.1"
                     /translation="MRHLVILSLLFPAWQATAGDIDRSIADFRTPAEIRWVRNPAGTN
                     EQAILHGDPSKPGPYVVRLKWLPGNMSRPHFHPNDRFFVVISGTWWVGTGDKFDPEST
                     VPVPAGSYVVHYGNKIHYDGAKGEECVIQVSGMGPATATPAEKK"
     gene            complement(1092..2171)
                     /locus_tag="VGO02_11095"
     CDS             complement(1092..2171)
                     /locus_tag="VGO02_11095"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEV7478268.1"
                     /translation="MKEHSIAVIPGDGIGREVIPEGVRVLEAAGARHGLRFKWQEFPW
                     SCDWYKAHGRMCPEDAPAILKKFDAVYFGAVGNPAIVPDHISAWGLLINFRRWFDLYV
                     NLRPVRLFEGLPCPLVGRKPGDIDYIVVRENTEGEYGNVGGRFNENTPDEIVMQQSIF
                     TRKGVDRIMRYSFELARSRKKHLTSCTKSNGITITMPYWDERFAEMKKTFPDIRTDQY
                     HVDGLTIQMVLNPERFDVIVASNLFGDILSDLGPATAGTIGIAASGNINPQRTFPSIF
                     EPVHGSAPDIAGRKIANPIAAIWSGALMLEHLGHAQAAADIVRGIERVIVEGPHTPDM
                     KGKAGTAEVGAAVAEAIRNQSRKAA"
     gene            2196..2864
                     /gene="tsaB"
                     /locus_tag="VGO02_11100"
     CDS             2196..2864
                     /gene="tsaB"
                     /locus_tag="VGO02_11100"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEV7478269.1"
                     /translation="MRFAALETSTDWCSVALWMDGEIHAAERRAGHRHSELVLPMLEE
                     LFKKTSTSPEVLDAIAFGAGPGSFTGLRIACGLAQGLALPRGLPVLGVPTLEALAEEC
                     GADRVVACLDARMREVYYAALEKAGGRWREIVPTQCVAPEHAPPPPGDGWVGCGSGFA
                     AFPGFLRNKIGCVNPEIHPSALAVARLAAPRLAAGEGVDAAAAAPLYIRDKVALTSQE
                     LAAR"
     gene            2861..3325
                     /gene="rimI"
                     /locus_tag="VGO02_11105"
     CDS             2861..3325
                     /gene="rimI"
                     /locus_tag="VGO02_11105"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEV7478270.1"
                     /translation="MSAVLKTVAALAAMREADLAEVMSIEEAIYSHPWTRGNFADSLV
                     AGYECRTWRVDAELVGYFILMVAAGEAHLLNLSIAPAWQRKGHGSALLREATGIARAR
                     GARGIFLEVRPSNSGAQALYTRFGFHKVAVRRGYYPALFGREDALVLTLALQ"
     gene            3301..3972
                     /locus_tag="VGO02_11110"
                     /pseudo
     CDS             3301..3972
                     /locus_tag="VGO02_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
     gene            complement(3969..>4360)
                     /locus_tag="VGO02_11115"
     CDS             complement(3969..>4360)
                     /locus_tag="VGO02_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HEV7478271.1"
                     /translation="MGLVVIVMNYVQQVWLAVILMVLIGALAGFFVVPMNALLQHRGH
                     HLVGAGRSIAVQNFNENTSILIMIALYSWLLNVGHSIHLVIGCFGVFVAATMSFVQWW
                     YLRNRRVHRDEMERLLAFARAEVPGKN"
BASE COUNT          722 a         1407 c         1473 g          758 t
ORIGIN      
        1 gtttgtgaat ttcgaaaaaa acttcatccc acagcaccat gccgtgcctc tgcgcattcg
       61 acttggtgac cagggtcaga tgcttgcgcg gccgcttcct cgccagctcg aatgcgaatt
      121 tatggattct ttctaccccg gagcgcgtaa acgtggagac ttccatggcg acttcattct
      181 ccagcccgcg gtgcacacgc ccgccggcgc cggagtattc gccttctgtg ttctcgcgga
      241 tgatgaccca gtcgatctca tgcgcatttt tcaaaacaga agaaacaccc ggcagcacgc
      301 gggaaggccg cacgttcgcg tactggtcga agccctggca gatcggcagg cgcaggcccc
      361 acagcgatac gtggtcgggc acgtccggcg cgcccaccgc gccgaacagg atggcgtcga
      421 aggtcttgag ccggtcgagg ccgccgtccg ggatatacgc gccggtcttc ttgtagcggg
      481 cagagctcca gtcgaaggac tcgaatttca gctcgaatcc ctcctttgag gcgagggcgg
      541 ccaggacgtc gacaccggcg ggaatgactt cgttgccgat tccgtcaccc gggatcgagg
      601 cgattcggta ggatttcacc ggttgaatct accaaatgag gagggcgtga tgaggcacct
      661 ggtcattctg agcctgcttt tccccgcgtg gcaggcaact gcgggcgaca tcgaccgcag
      721 catcgccgat ttcaggacgc cggcggagat ccgctgggtg cgcaacccgg ccgggaccaa
      781 cgagcaggcg atcctgcacg gcgatccgtc gaagcccggg ccgtatgtcg tgcgcctgaa
      841 gtggctgccc ggcaacatga gccggccgca cttccatccg aacgaccgct tcttcgtggt
      901 gatctcgggc acctggtggg tgggcaccgg cgacaagttc gacccggaga gcacggtgcc
      961 cgtgccggcc ggtagctacg tcgtccacta tggcaacaag atccactacg acggcgcgaa
     1021 gggcgaagag tgcgtgatcc aggtctccgg catgggtccc gcgactgcca cgcccgcgga
     1081 gaagaagtag ctcaggccgc ttttctggat tgatttctta tcgcttcggc gaccgcggcg
     1141 ccgacctccg cggtgcccgc cttgcctttc atgtcggggg tgtgcggccc ttcgacgatg
     1201 acgcgctcga tgccgcgcac gatatcggcg gcggcctggg catggccgag gtgctcgagc
     1261 atcagcgcgc ccgaccagat ggcggcgatc gggttggcga tcttcctgcc ggcgatatcg
     1321 ggcgccgagc cgtgcaccgg ctcgaagatc gacgggaaag tgcgctgcgg attgatgttg
     1381 cccgaggcgg cgatgccgat cgtgccggcg gtggccgggc cgaggtcgga gagtatgtcg
     1441 ccgaacaggt tggaggcgac gatgacgtcg aagcgctccg ggttgagcac catctggatg
     1501 gtgaggccgt cgacgtgata ctgatccgtt ctgatgtcgg ggaaagtttt cttcatctcg
     1561 gcgaagcgct cgtcccagta cggcatggtg atggtgatgc cgttcgactt ggtgcacgag
     1621 gtgaggtgct tcttgcggct gcgcgccagc tcgaatgagt agcgcatgat gcggtcgacg
     1681 cccttgcggg tgaagatcga ctgctgcatg acgatttcgt ctggggtatt ttcattgaag
     1741 cgaccgccca cgttgccgta ctctccctcc gtgttctcgc gcaccacgat gtagtcgatg
     1801 tcgccgggct tgcgtccgac gagcgggcag ggcaggccct cgaacaggcg caccgggcgc
     1861 aggttgacgt acaggtcgaa ccagcggcgg aagttgatca gcagccccca cgcggagata
     1921 tggtcgggga cgatcgccgg attgccgacg gcgccgaagt acaccgcgtc gaacttcttc
     1981 aggatggccg gcgcgtcctc cgggcacatg cggccgtgcg ccttgtacca gtcgcagctc
     2041 cacgggaatt cctgccactt gaagcgcagg ccgtgccgcg cgccggccgc ctcgagaacg
     2101 cgcacgcctt cggggatgac ttcacgcccg atgccgtcgc ccgggatgac tgctatcgaa
     2161 tgttccttca tgcccgcgag tatatagaat ccgccatgcg cttcgcggct ctcgaaacct
     2221 cgaccgattg gtgctcggtg gccctttgga tggacggcga gatccacgcc gcagagcggc
     2281 gcgccggaca ccgccattcg gagctggtgc tgccgatgct cgaagaattg ttcaagaaaa
     2341 caagtaccag ccctgaagtt ctggatgcca ttgccttcgg ggcgggtccc ggttcgttta
     2401 ccggcctgcg catcgcgtgc ggcctcgcgc agggcctcgc cctgccgcgc ggcctgccgg
     2461 ttctcggcgt gccgacgctc gaggcgctcg ccgaggagtg cggcgccgat cgcgtggtgg
     2521 cctgcctcga tgcgcgcatg cgcgaggtct actatgcggc gctcgaaaag gccggcgggc
     2581 gctggcgcga gatcgtcccc acgcagtgcg tggcgcccga gcacgcgccc ccgccgccgg
     2641 gagacggctg ggtcggttgc ggcagcggct ttgccgcgtt cccggggttt cttcgcaata
     2701 aaatcggctg cgtgaacccg gagatccatc ccagcgccct cgccgtggcg cgcctcgcgg
     2761 cgcccaggct ggcggcaggc gagggcgtcg acgcggcggc cgccgcgccg ctctacatcc
     2821 gcgacaaggt cgcgctcacc tcgcaggagc tggccgcgcg atgagcgccg tcctcaagac
     2881 cgttgccgcg ctcgcggcga tgcgcgaggc ggacctcgcc gaggtgatgt cgatcgagga
     2941 ggccatctac agccacccct ggacgcgcgg caattttgcc gactcgctgg tcgcgggcta
     3001 cgagtgccgc acctggcgcg tggacgcgga gctggtcgga tacttcatcc tgatggtggc
     3061 ggccggcgag gcgcacctgc tcaacctgtc gatcgcgccg gcctggcagc gcaagggcca
     3121 cggcagcgcg ctgctgcgcg aggcgacggg catcgcgcgt gcgcgcggcg cgcgcggcat
     3181 ctttctcgaa gtgcggccga gcaactcggg cgcgcaggcg ctctacacgc gcttcggctt
     3241 tcacaaggtc gccgtgcggc gcggctacta tccggcgctt ttcgggcgcg aggacgcgct
     3301 ggtgctgact ctcgcgctgc aatgagccgc cgcgacgagg tgctggccga gatgggtttt
     3361 tcccctatct ggaagcttcg caacaatccc gttccaatac aaaaattttc gaatcaagga
     3421 aaaaattggg ttgagctgaa gcaggcggtt ccggcatgca ccgcatgcgg cctgcacaag
     3481 ggccgcaccc agaccgtgtt cggcgtcggc gacgagcagg ccgactggct gctggtgggc
     3541 gaggcccccg gcgccgagga agaccggctg ggcgatccgt tcgtcggcca ggcgggcaag
     3601 ctgctggaca acatgctcgc cgcgatcggc ctggcgcgcg acgacaacgt ctatatctgc
     3661 aacgtgctga agtgccggcc gccgggcaac cgcaacccgg agcccgacga ggtggcgaag
     3721 tgcacgccgt tcctgctgcg ccagatcgag ctgatcgagc ccaggctgat cgtggcgatg
     3781 ggccgcttcg cggcgcagac gctgctcgat accgatgcca gcatcgccag cctgcgcggg
     3841 cgcgtgcacc agtacgccgg ccggccgctg atcgtcacgt accacccggc ttatctgctg
     3901 cgcaacctgc cggacaagtc caaggcgtgg gcggaccttc tcttcgcccg cgggacgatg
     3961 tcgactctct agttcttgcc ggggacttcg gcgcgggcga aggcgagcag ccgctccatt
     4021 tcgtcgcggt gcacgcgccg gttgcgcagg taccaccact gcacgaaact catggtggcc
     4081 gcgacgaaca cgccgaagca gccgatcacc aggtggatcg agtggccgac gttcagcagc
     4141 cacgagtaca gcgcgatcat gatcaggatc gaggtgttct cgttgaagtt ctgcaccgcg
     4201 atcgagcggc cggcgccgac caggtggtgg ccgcggtgct gcagcagcgc gttcatcggc
     4261 acgacgaaga acccggcgag ggcgccgatc agcaccatca ggatcaccgc cagccagacc
     4321 tgctgcacgt agttcatgac gatcaccacc aggcccatga
//
LOCUS       DASYJA010000514         1904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3074377, whole genome shotgun sequence.
ACCESSION   DASYJA010000514 DASYJA010000000
VERSION     DASYJA010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3074377"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /gene="hisD"
                     /locus_tag="VGO02_11120"
     CDS             complement(<1..1222)
                     /gene="hisD"
                     /locus_tag="VGO02_11120"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEV7478272.1"
                     /translation="MPQLRRLSSVAPGFDAKLAALTRYDAAQDPAVQKTVRRILADVR
                     KRGDAAVREYTKKFDGVSPKEFSVSEKFISAIPEEQAAALRAAHQRIRAFHEKQLQKS
                     WDFTDADGTRLGQQVSALERVGLYVPGGKAAYPSSVLMNAVPAKVAGVRELVMASPNP
                     TPLVLAAAALAGVDRVLGIGGAQAIGAMAYGTKSIARVDKIVGPGNAYVAEAKRQVFG
                     AVGIDMVAGPSEILVIADSSANPDWVAMDLFSQAEHDESAQAILLSPDERFLDAVAAS
                     IEKLIVEMPRRKVIAASLKARGALILTRDLEEACALANRIAPEHIEIAVQFPERFLAK
                     IRNAGAIFLGHWSSEAIGDYCAGPNHVLPTAGTARFSSPLGVYDFQKRTSVIAVSKES
                     AVKLGRIAMILAEGE"
     gene            complement(1228..1872)
                     /gene="hisG"
                     /locus_tag="VGO02_11125"
     CDS             complement(1228..1872)
                     /gene="hisG"
                     /locus_tag="VGO02_11125"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEV7478273.1"
                     /translation="MASISIALSKGRIFDETAPLLARAGIRPRQDPEHSRKLIIGTNQ
                     RDLRLIVVRASDTPTYVQHGGADIGIAGGDVLVEHGGEGLYQPVDLGIARCRMMVAVR
                     KGFDYAGAVRQGMRLRVATKYVNITRQHFGDKGVHVDLIRLYGSMELAPLTGLADAIV
                     DLVATGSTLKANDLVAVEEIMPVSARLIVNQAALKTRRAELQPLIEAFANAVRK"
BASE COUNT          285 a          644 c          649 g          326 t
ORIGIN      
        1 cttcgccttc ggcgaggatc atcgcgatgc gtcccagctt gacggcggat tccttcgaca
       61 ccgcgatcac gctggtgcgc ttctggaagt cgtagacccc gagcggcgag gagaagcgcg
      121 ccgtgccggc ggtcggcagc acgtggttcg gcccggcgca gtagtcgccg atcgcctccg
      181 agctccagtg accgaggaag atcgcgccgg cattcctgat cttggcgaga aatctttcgg
      241 gaaactgaac cgcaatttcg atgtgctccg gcgcgatgcg gttggcgagc gcgcaggctt
      301 cctcgaggtc gcgcgtcagg attagcgcgc cgcgcgcctt cagcgatgcg gcgatgactt
      361 tccgcctcgg catctcgacg atcagtttct cgatggaagc ggcaacggca tccagaaacc
      421 gctcatccgg cgagagcagg atcgcctgcg ccgactcgtc gtgctcggcc tgcgagaaga
      481 gatccatcgc cacccagtcg ggattggccg agctgtcggc gatcaccaga atctcggagg
      541 gcccggcgac catgtcgatg ccgaccgcac cgaacacctg gcgcttcgcc tcggcgacgt
      601 aggcgttgcc gggaccgacg atcttgtcta cgcgcgcgat cgacttggtg ccgtaggcca
      661 tcgcgccgat cgcctgcgcg ccgccgatgc cgagcacgcg gtcgacgccg gcgagcgctg
      721 cggcggcgag caccagcggg gtggggttgg ggctcgccat gaccagctcg cgcacgcccg
      781 ccaccttcgc cggcaccgcg ttcatcagca ccgacgaggg ataggccgcc ttgccgccgg
      841 gcacgtacag gccgacgcgc tcgagggcgc tcacctgctg gccgaggcgc gtgccgtcgg
      901 cgtcggtgaa atcccacgac ttctgcagct gcttctcgtg gaacgccctg atgcgctggt
      961 gcgccgcgcg caaggccgcc gcttgctctt ccggtatggc agaaataaat ttttcagaaa
     1021 cagaaaattc cttgggcgac acgccgtcga atttcttcgt gtactcgcgc accgccgcgt
     1081 cgccgcgctt gcgcacgtcg gcgaggatcc tgcgcaccgt cttttgcacc gcgggatcct
     1141 gcgccgcgtc gtagcgcgtg agcgcggcga gcttggcgtc gaagccgggc gcgacgctcg
     1201 acaggcggcg cagctgcggc attttcgtca tttgcggacc gcgttcgcga acgcctcgat
     1261 gagcggctgc agctccgcgc ggcgcgtctt gagcgccgcc tggttgacga tcaggcgcgc
     1321 cgacaccggc atgatctcct cgaccgcgac caggtcgttc gccttcagcg tgcttccggt
     1381 ggcgacgagg tcgacgatgg cgtcggccag gccggtgagc ggcgcgagct ccatcgagcc
     1441 gtataggcgg atgaggtcga cgtgcacgcc cttgtcgccg aagtgctggc gggtgatgtt
     1501 gacgtacttg gtggcgaccc gcaggcgcat tccctggcgc accgcgccgg cgtagtcgaa
     1561 gcccttgcgc accgccacca tcatgcggca gcgggcgatg cccaggtcga ccggctggta
     1621 caggccctcg ccgccgtgct cgaccagcac gtcgccgccc gcgatgccga tgtcggcgcc
     1681 gccgtgctgc acgtaagtgg gcgtgtcgga ggcccgcacc acgatcaagc gcaggtcgcg
     1741 ctggttcgtg ccgatgatga gcttgcggga gtgctcgggg tcttgcctag gccggatacc
     1801 ggcgcgcgcg agcagcggcg cggtctcgtc gaagatccgc cccttcgaca acgcaatgct
     1861 gatgctcgcc atgtgccata tccggatgcg tcagtgccag tctc
//
LOCUS       DASYJA010000515         5317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2620505, whole genome shotgun sequence.
ACCESSION   DASYJA010000515 DASYJA010000000
VERSION     DASYJA010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2620505"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..613)
                     /locus_tag="VGO02_11130"
     CDS             complement(26..613)
                     /locus_tag="VGO02_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV7478274.1"
                     /translation="MKTLQINSSIFGAGGQSSQLADKLVAGIGGDVVVRDLARDPVPH
                     LDAERFGAFLAKPGARTAQQQAVIDYSDRLIGELREADTIVIALPMYNFGVPSMLRAY
                     FDHVARAGVTFKYTEKGPVGLLTGKKAYVFVTRGGVYGEKHAHTDYVRDFLAFLGITD
                     VTFIHAEGLAISEPSKQASLVKARNRIAELELIAA"
     gene            686..1624
                     /locus_tag="VGO02_11135"
     CDS             686..1624
                     /locus_tag="VGO02_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV7478275.1"
                     /translation="MSPRITLEQWQALVAVVDAGGYAQAAARLHKSQSSVTYLVQELQ
                     RRLSVKAFEIKGRKAALTPTGELLYRRARLLLEEAAGLEKAAKSASAGWEAEIRIAAE
                     IIFPTWLLLQCFDRLNVESPHTRIELIESVIAGTNEALLEGRADLAITPQVPAGFDGT
                     PLMQARMVLVAHPEHALHKLGRPVTMRDLRAHRQLVVRESDARRATKTQLEADQRWTV
                     SHMATSIFAAAMGFGYSWFPEERIRGELQAGSLKPLPMRERGGERFGQLYLVFADREN
                     AGPGTLRLGALIEEMVAAECTREESHANRRHRRKPA"
     gene            1590..2135
                     /locus_tag="VGO02_11140"
     CDS             1590..2135
                     /locus_tag="VGO02_11140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010811772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEV7478276.1"
                     /translation="MRIVGIAGSLRSGSFNAALLRAAQEEAPAGMALEIESIRGIPLY
                     DGDVENAQGLPARVVELKDKIASADALLLVTPEYNNSIPGVFKNAIDWMSRPAADIDR
                     VFGARPVGLIGASPGRQGTVLSQTAWLQVFRALGLRPWFGQLLYVGGAGSVFDASGKL
                     VDEATRARLRKYLEGFAAFVA"
     gene            2157..3398
                     /locus_tag="VGO02_11145"
     CDS             2157..3398
                     /locus_tag="VGO02_11145"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV7478277.1"
                     /translation="MAVKEEFMKLLSLVAGLLFSALVAAQQGPIKIGMSMPQTGSLGA
                     GGQAALLALNMWVEDINAKGGLLGRKVELIAYDDQTNPALTPAIYTKLLDVDKVDLLI
                     APYGTVPTAPIMPLVKQRDLLLMGNFSFQANHTVKHDKWFNNAPWNNASSWSDGFFAI
                     GQKQGAKTVAFLAADNEFAQNLANGARELAKKAGLQTVYNQNYPPTTTDFSSLIRGVR
                     AAKPDMVFVMSYPNDSVAIVRAVNEIGVGDSVKLFGGGMVGLQFTPIMTNLGSSLNGI
                     VNYNSYVPGIKYPGIEEFLQRYSKRAVDAKVDPLGFYLPPFNYAIGQMLEQAINATKS
                     LDHKKLAEYLHNNELKTMVGPIKYGPDGEWASPRVVQAQFRGVADKNMEQFRQSDKQV
                     VLYPDAYKTGEVVAPFEKARK"
     gene            3472..4350
                     /locus_tag="VGO02_11150"
     CDS             3472..4350
                     /locus_tag="VGO02_11150"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV7478278.1"
                     /translation="MSFDLLLEAVLFGILLGCFYAAVSMGLSVSFGLLDVPHVAHPAI
                     MVLGAYCTWMLGTWGIDPILAGVLLMPPFFLLGMVIYRFYYEAFEKRGSDAGVRGIAF
                     FFGVALIIEVGLILAFGVDQRMVEAPYIGKSINIGWDIRLPMRTLAVFAVAIAMSVGL
                     TLYMSKTFTGRAIKAVAQDEPALRLMGANPVRIKQWAFGIATAVAALGGAMLIIIGPV
                     DPGLDRVYIGRTFAVVVLAGLGSMTGTIAAGLILGISESIILIHPDLGASWAPAVSFL
                     LLLLVLGVRPQGLFGR"
     gene            4347..5282
                     /locus_tag="VGO02_11155"
     CDS             4347..5282
                     /locus_tag="VGO02_11155"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV7478279.1"
                     /translation="MKFWIGIAITLAITLATVAFVNFDYFFFAGYTVLMFVMLATAWN
                     ILGGYAGYVNFGTPAFFGLGAYTAAVLYKATAAPLVLQILAAAAMSGLLGLVAGLLTL
                     RLRGIFFAIATIALVVIMETLFINWRYAGGATGLQLLRPPLTWPFESYIKMLFLVMAV
                     MVLASIAVARYIERSWFGRGLRALRDSEEAAECSGVPTLRLKVFACTVSGALMGACGA
                     PMPMYLSFIEPASTFNLNYAIGALASPMIGGTAHWLGPVIGALILSSLQQLVTVTISN
                     EMNVLVVGVLLVVFVVAAPDGILGLYKKIRTAVKK"
BASE COUNT          871 a         1756 c         1767 g          923 t
ORIGIN      
        1 tccaacgccg cccgcctgct cggcatcaag ccgcgatgag ctcgagctcg gcgatccggt
       61 tgcgggcttt cacgagggaa gcctgcttgg agggctcgct gattgcgagt ccttccgcgt
      121 ggatgaaggt gacgtcggta atgccgagga aggcaaggaa gtcgcgcacg taatccgtgt
      181 gcgcgtgttt ctcgccgtag accccaccgc gcgtcacgaa gacgtaggcc ttcttgccgg
      241 tgagcaggcc gaccgggccc ttctcggtgt acttgaaggt gacgccggcg cgcgccacgt
      301 ggtcgaaata cgcgcgcagc atggagggca cgccgaagtt gtacatcggc agcgcgatga
      361 cgatcgtgtc ggcctcgcgc agctcgccga tcagccggtc cgagtagtcg atgaccgcct
      421 gctgctgcgc agtgcgcgcc ccgggcttcg ccaggaatgc gccgaagcgc tccgcgtcca
      481 ggtggggaac cgggtcacgc gcgagatcgc gcaccaccac gtcgccgccg ataccggcca
      541 ccaacttgtc cgcgagctgg ctcgactggc cgcccgcgcc gaagatgctg ctgttgatct
      601 gtagggtttt catgccttca tttaagtatc tatcgagacg ataaaaaaga ggaaaatacc
      661 gtccataccc atcgggtagg ccgatatgtc ccctcgcatc accctcgagc aatggcaggc
      721 cctggtggcc gtggtggacg ccggtggcta cgcgcaagcc gcggcgcgcc tgcataagag
      781 ccagtcctcg gtgacctacc tggtgcagga gttgcagcgg cggctcagcg taaaggcgtt
      841 cgagatcaag gggcgcaagg cggcgctgac gccgaccggc gagcttctgt accggcgcgc
      901 gcgcctgctg ctggaggagg cggcgggcct ggagaaggcg gcgaagtccg cgtccgcggg
      961 ctgggaggcc gagatccgca tcgccgccga gatcatcttc cctacctggc tgctgctgca
     1021 gtgcttcgac cgcctgaacg tggaaagccc gcatacgcgc atcgagctga tcgagtcggt
     1081 gatcgcgggg accaacgagg cgctgctcga gggacgcgcc gacctcgcca tcactccgca
     1141 ggtgccggcg ggcttcgacg gcacgccgct catgcaggcg cgcatggtgc tggtcgcgca
     1201 cccggaacat gcgctgcaca agctggggcg cccggtgacc atgcgcgatt tgcgcgcgca
     1261 ccgccagctg gtcgtgcgcg agagcgacgc gcgccgcgcg accaagaccc agttggaggc
     1321 cgaccagcgc tggaccgtga gccacatggc gacctcgatc ttcgccgccg cgatgggctt
     1381 cggctacagc tggttccccg aggagagaat tcgcggcgag ctgcaggccg gctcgctcaa
     1441 gccgctgccg atgcgcgagc gcggcggcga gcgcttcggg cagctttacc tggtgttcgc
     1501 cgaccgcgag aatgcgggac ccggcacctt gcgcctgggc gcgctcatcg aggagatggt
     1561 tgcggccgaa tgcacccggg aggaaagcca tgcgaatcgt cggcatcgcc ggaagcctgc
     1621 gtagcggatc gttcaacgcg gcgctgctgc gcgctgcgca ggaagaggct cccgcgggca
     1681 tggcactcga gatcgaatcg atccgcggca ttccgctgta cgacggcgat gtcgagaacg
     1741 cgcagggcct tccggcgcgc gtcgtggagc tgaaggacaa gatcgcgtct gccgacgcgc
     1801 tgctcctcgt cacgccggag tacaacaact cgattcccgg cgtgttcaag aacgccatcg
     1861 actggatgag ccggcccgcc gccgacatcg accgcgtgtt cggcgctcgc ccggtgggat
     1921 tgatcggcgc ctcgcccgga cgccagggca cggtgctgtc gcaaaccgcc tggctgcagg
     1981 tgttccgcgc gctgggcctg cgcccatggt tcggccagct tctctatgtg ggcggcgcgg
     2041 gcagcgtgtt cgacgcctcc ggcaagcttg tcgacgaagc cacccgcgcg cggctgcgaa
     2101 agtacctgga gggcttcgcc gcgttcgttg cataaatcac ggccggattg gctacaatgg
     2161 ccgtcaagga ggagttcatg aaactactat cactcgtagc gggtctgctg ttcagcgcgc
     2221 tcgttgcggc gcagcagggg cccatcaaga tcggcatgag catgccgcag accggctcgc
     2281 tcggcgccgg cggccaggcg gcgctgctcg cgctcaacat gtgggtcgag gacatcaacg
     2341 ccaagggcgg cctgctcggg cgcaaggtcg agctgatcgc ctatgacgac cagaccaacc
     2401 cggcactcac gccggcgatc tacaccaagc tgctcgacgt cgacaaggtc gacctgctga
     2461 tcgcgcccta tgggacggtg cccacggcgc cgatcatgcc gctggtcaag cagcgcgacc
     2521 tgctgctgat gggcaatttc tccttccagg ccaaccacac cgtcaagcac gacaagtggt
     2581 tcaacaacgc gccgtggaac aacgcctcga gctggtccga cggcttcttc gccatcggcc
     2641 agaagcaggg cgcgaagacc gtggcgttcc tcgccgccga caacgagttc gcgcagaact
     2701 tggcgaacgg cgcgcgcgag ctggcgaaga aggccggcct gcagacggtg tacaaccaga
     2761 actacccgcc gaccacgacc gacttctcct cgctgatccg cggcgtgcgc gccgccaagc
     2821 cggacatggt gttcgtcatg tcctacccga acgactcggt ggccatcgtg cgcgcggtga
     2881 acgagatcgg cgtcggcgac tcggtcaagc tgttcggcgg cggcatggtg gggctgcagt
     2941 tcacgccgat catgaccaac ctcggctcgt cgctcaacgg catcgtcaac tacaactcct
     3001 acgtgccggg catcaagtac ccgggcatcg aggagttcct gcagcgctac tcgaagcgcg
     3061 cggtggacgc gaaggtcgac ccgctcggct tctacctgcc gccgttcaac tatgccatcg
     3121 gccagatgct cgagcaggcg atcaacgcca ccaagagcct cgatcacaag aagctcgccg
     3181 agtacctgca caacaacgag ctgaagacca tggtcggccc catcaagtac ggccccgacg
     3241 gcgagtgggc cagcccgcgc gttgtgcagg cgcagttccg cggcgtggcc gacaagaaca
     3301 tggagcagtt ccgccagagc gacaagcagg tggtgctgta ccccgacgcg tacaagacgg
     3361 gcgaggtagt cgcgccgttc gaaaaagcgc ggaagtaggg caactattgg gaaaaacggc
     3421 gcggcgatgc cgcgcctttt ttttgcgtcg gatttcttgg tctaggggaa cgtgagcttc
     3481 gacctgctgc tcgaggcggt gctgttcggc atcctgctcg gctgcttcta tgccgcggtg
     3541 agcatgggcc tgtcggtgtc gttcggcctg ctcgacgtgc cgcacgtcgc ccacccggcg
     3601 atcatggtgc tgggcgccta ctgcacctgg atgctcggca cctgggggat cgacccgatc
     3661 ctcgccggcg tgctgctgat gccgccgttc ttcctgctcg gcatggtcat ctaccgcttc
     3721 tactacgagg ccttcgagaa gcggggctcc gacgcgggcg tgcgcggcat cgccttcttc
     3781 ttcggcgtgg cgctgatcat cgaggtcggg ctgatcctcg ccttcggcgt cgaccagcgc
     3841 atggtcgagg cgccctacat cggcaagagc atcaacatcg gctgggacat ccgcctgccg
     3901 atgcgcaccc tggcggtgtt cgcggtcgcg atcgccatgt ccgtggggct cacgctgtac
     3961 atgtcgaaga cctttaccgg ccgcgcgatc aaggccgtcg cccaggacga gccggcgctg
     4021 cgcctgatgg gcgcgaaccc ggtgcgcatc aagcaatggg ccttcggcat cgccaccgcg
     4081 gtggccgcgc tcggcggcgc gatgctgatc atcatcgggc cggtcgaccc gggcctcgac
     4141 cgcgtctaca tcgggcgcac cttcgcggtg gtggtgctcg ccggcctggg cagcatgacc
     4201 ggcaccatcg ccgccgggct gatcctcggc atctccgagt cgatcattct gatccacccc
     4261 gatctgggcg cgtcgtgggc gccggcggtg tcgttcctgc tcctcctgct ggtgctcggc
     4321 gtgcgcccgc agggattgtt tggccgatga aattctggat cggcatcgca atcacgctcg
     4381 ccatcaccct cgccacggtg gcgttcgtca atttcgacta cttcttcttc gccggctaca
     4441 cggtgctgat gttcgtcatg ctggctaccg cctggaacat cctcggcggc tacgccggct
     4501 acgtgaactt cggcacgccg gcgttcttcg gcctgggcgc ctacaccgcc gcggtactct
     4561 ataaggccac cgcggcgccg ctggtgctgc agattctcgc cgcggcggcg atgagcggac
     4621 tgctcggcct ggtcgccggg ctgctgacgc tgcgcctgcg cggcatcttc ttcgccatcg
     4681 ccaccatcgc gctggtggtg atcatggaga cgctgttcat caactggcgc tacgccggcg
     4741 gcgccaccgg cctgcagctg ctgcgcccgc cgcttacctg gccgttcgag tcctacatca
     4801 agatgctgtt cctggtgatg gcggtcatgg tgctggcgtc gatcgccgtg gcgcgctaca
     4861 tcgagcgctc ctggttcggc cgggggctgc gtgcactgcg cgacagcgag gaggccgccg
     4921 aatgcagcgg cgtgcccacg ctcaggctca aggtgttcgc ctgcaccgtg tcgggcgcgc
     4981 tgatgggggc ctgcggcgcg ccgatgccga tgtatctcag cttcatcgag ccggcgtcga
     5041 ccttcaacct caactacgcc atcggcgcgc tggcgagccc gatgatcggc ggcaccgcgc
     5101 actggctcgg gccggtgatc ggcgcgctga tcctcagctc gctgcagcag ctcgtcaccg
     5161 tcaccatctc gaacgagatg aacgtcctgg tggtgggggt gctgctggtg gtgttcgtgg
     5221 tcgccgcgcc cgatggaatt ctgggtttat acaaaaaaat aagaacagcc gtaaaaaaat
     5281 gacgacccag ctcctgaaaa tccagaacgt gtcgaag
//
LOCUS       DASYJA010000516         1910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3074664, whole genome shotgun sequence.
ACCESSION   DASYJA010000516 DASYJA010000000
VERSION     DASYJA010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3074664"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..808)
                     /locus_tag="VGO02_11160"
     CDS             complement(71..808)
                     /locus_tag="VGO02_11160"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV7478280.1"
                     /translation="MSGPLPFELTALRSAVNYQRWVADAAAPHLGARILEVGAGLGNL
                     SRWLPLHERLVLSEREPELLRLLRAEVRERFGADPRVAVVQADLGGELPAAIAAEDLD
                     TIVSFNVLEHVEDDVGVLARLAALLLRSRCPGPRRIVSFVPAHPWAYGSVDAAYGHVR
                     RYTAASFAALAAKSCPDWQLETRYFNAFGLPGWVLLGRLLRRQSVSAGAIRWFERLCP
                     WIRGADDALHRVGLPFGQSLVAVLTHP"
     gene            complement(822..1862)
                     /locus_tag="VGO02_11165"
     CDS             complement(822..1862)
                     /locus_tag="VGO02_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HEV7478281.1"
                     /translation="MKILILGVNGFIGHHLSHRIMADTDWEVYGMDMQSDRVTDLLDE
                     KRFRFFEGDITINREWIEYNIRKCDVILPLVAIATPATYVKEPLRVFELDFEANLPIV
                     RSCVKHGKRLIFPSTSEVYGMCPDARFDPETSPLVYGPINKPRWIYACLKQLMDRVIA
                     AYGQSANFDYTLFRPFNWIGPGLDSINTAKEGSSRVITQFLGHIVRGERIKLVDGGRQ
                     KRAFTFVDDGIDALMKIIENRNGIARHKIYNVGNPRNNLSIKQLAQMMLKLALTYPEY
                     AKQAKKVRIVKTSSAQYYGKGYQDMQNRVPHIANTSRDLNWRPRVGMKDAVTRVLEAY
                     RTQVAEARRLNR"
BASE COUNT          315 a          606 c          638 g          351 t
ORIGIN      
        1 cacctgcctg atgctgcttt cctcgttgta cgccggaatc acgacgctaa gaaccgtcat
       61 tgctctccct tcatggatgc gtgaggacgg cgaccagcga ttggccgaac ggcaagccca
      121 cgcggtgcag cgcgtcgtcc gctccgcgga tccacgggca cagccgctcg aaccagcgga
      181 tcgcgccggc gctgaccgat tgccggcgca gcaacctgcc gagaagcacc cacccaggca
      241 gcccaaaggc gttgaagtag cgcgtctcga gctgccaatc cgggcagctc ttcgccgcga
      301 gggcggcgaa gctggcagcg gtgtagcgcc gcacgtggcc gtaggcggcg tcgacactgc
      361 cgtaggccca gggatgcgcc ggcacgaaac tcacgatgcg gcgcgggcct ggacaacggc
      421 tgcgcagcag cagcgcggca agccgcgcga gcactccaac atcgtcctcg acgtgctcga
      481 gcacgttgaa ggacacgatc gtatccaggt cctccgccgc gattgccgcc ggcaattcgc
      541 cgccgaggtc ggcttgcacc acggcgacgc gtggatcggc accaaagcgc tcgcgcactt
      601 cggcgcggag cagccgcagc agctcgggct cgcgctcgct cagcaccagc cgctcatgca
      661 acggcagcca gcgcgacagg ttgccgaggc ctgcgccgac ctcgagaatg cgcgcgccga
      721 ggtgaggtgc ggccgcgtcg gcgacccagc gctggtagtt gaccgcgcta cgcagtgcgg
      781 tgagctcgaa gggcagcggc ccgctcatgc gctcagggtg atcatcggtt caggcgccgc
      841 gcctcggcga cctgcgtgcg ataggcttcg agcacccgcg taaccgcgtc cttcataccc
      901 actctgggcc gccagttcag gtcgcggctg gtgttggcga tgtggggcac gcggttctgc
      961 atgtcctggt agcccttgcc gtagtactgc gccgagctgg tcttgacgat gcgcaccttt
     1021 ttcgcctgct tggcgtactc ggggtaggtc aaggcgagct tcagcatcat ctgggcgagc
     1081 tgcttgatcg acaggttgtt cctcgggttg ccgacgttgt agatcttgtg gcgagcgatg
     1141 ccgttcctgt tctcgatgat cttcatcagc gcgtcgatgc cgtcgtcgac gaaggtgaag
     1201 gcgcgcttct gccgcccgcc gtcgaccagc ttgatgcgct cgccgcgcac gatgtggccg
     1261 aggaactggg tgatgacgcg cgagctgcct tccttcgccg tattgatgga gtccaggccg
     1321 ggcccgatcc agttgaacgg ccggaacagg gtgtagtcga aattcgcgct ctgcccgtac
     1381 gccgcgatga cgcggtccat cagctgcttc aggcaggcgt agatccagcg cggcttgttg
     1441 atcgggccgt agaccagcgg cgaggtctcc gggtcgaacc tggcgtccgg gcacatgccg
     1501 tacacctcgg aggtggaagg aaagatcagg cgcttgccgt gcttcacgca cgatcgcacg
     1561 atcggcaggt tggcctcgaa gtcgagctcg aacacgcgca gcggctcctt cacgtaggtg
     1621 gccggcgtgg cgatcgccac cagcggcagg atgacgtcgc acttgcggat gttgtattcg
     1681 atccactccc ggttgatggt gatgtcgcct tcgaagaagc ggaagcgctt ctcgtccagc
     1741 aggtcggtga cgcggtcgga ctgcatgtcc atgccgtaca cctcccagtc ggtgtcggcc
     1801 atgatccggt gcgagaggtg gtgtccgatg aagccgttca cgccgaggat caggattttc
     1861 atcttggttc tttcttatct gaaggtagat gatatattga ccggcggatg
//
LOCUS       DASYJA010000517         3404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2893811, whole genome shotgun sequence.
ACCESSION   DASYJA010000517 DASYJA010000000
VERSION     DASYJA010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2893811"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1044)
                     /gene="gcvA"
                     /locus_tag="VGO02_11170"
     CDS             complement(133..1044)
                     /gene="gcvA"
                     /locus_tag="VGO02_11170"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HEV7478282.1"
                     /translation="MRNRNALPALDLLVGFEAAARHLSFTKAGEELYLTQSAVSRQIK
                     ELEDQLGVPLFQRRHRALTLTAPGQQFYAAASQVISTMRTATERLRSQAGRRRPLSVT
                     TTNSFASLWLIPRLAGFTRDHPGIDVRITAETKVQDLESEGLDIALRHGPAALAGPDA
                     VRLFGEKVFPVASPKLLKRLPLDKPADLRHHILLHYDDPDARHPWLHWKTWLEVERIA
                     DLRPAGTLSFSGFEQIIPAAVAGHGVGLGRSPLVKDLLASGELVAPFKSQADPARGYY
                     AVVSRHAADRPEVAAFVEWLKREAAKR"
     gene            1123..1353
                     /locus_tag="VGO02_11175"
     CDS             1123..1353
                     /locus_tag="VGO02_11175"
                     /inference="COORDINATES: protein motif:HMM:NF022592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HEV7478283.1"
                     /translation="MKIELEAAAVRLTPNQTLKLRDSAGSTVHAVEGSVWITEENQPR
                     DVVLGPGGCYRLKHAGLAIINALGGGAAVSFS"
     gene            1364..1507
                     /locus_tag="VGO02_11180"
     CDS             1364..1507
                     /locus_tag="VGO02_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478284.1"
                     /translation="MRPDLAELHAAARRQRAIEIHRLIFLPIAAFFRRPSKKEQLRVA
                     ICH"
     gene            1498..1896
                     /locus_tag="VGO02_11185"
     CDS             1498..1896
                     /locus_tag="VGO02_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEV7478285.1"
                     /translation="MPLVRISALQGPEGFGRKVGQVVYRTMVDTIDVPRDDNFQVITE
                     HPRDTLVFDRTYMGIARSDGFVSIQITLSEGRSVAKKRLFYRTLAERLQRELNVRPED
                     VFVNLVEVKKENWSFGNGVASYAPAESVAA"
     gene            1898..3109
                     /locus_tag="VGO02_11190"
     CDS             1898..3109
                     /locus_tag="VGO02_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7478286.1"
                     /translation="MPSFGARAGLIALCFGNFVIGTGTLIVPGMLPQLAEGLGVSLPI
                     AGQLVTAFAAAVCLGAPLLAGVTSRLDRRALLVAMQLVFVAGHLAAALVSSFWPMLAV
                     RVLTSVGAALFTAQAASAAALLVPAEARGRAIAFVFLGWSIASVAGLPLGSYVSAVWG
                     WRAGFGLVAAGAAFGAAAVWLLLPRGLRVEPVTSAMWRSILRDPALMASVGVTALFAG
                     AGFALFAYFVPAARSYLGASPELVSGLLAVFGIAGLAGNMLAVRYMDRLGAANVVMLC
                     LLSMLAAQLLWPWSQGSVTLVALVMIGCGLGVFACNSAQQTRLAAIAPAAASVSIALN
                     SSAIYLGQALGPAAGGVLIAHVPGNEGYALIAAIGVPLLLAAIGLSYFASLRMRARPR
                     GVHVQGRPVPG"
     gene            3081..>3404
                     /locus_tag="VGO02_11195"
     CDS             3081..>3404
                     /locus_tag="VGO02_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7478287.1"
                     /translation="MFKEGLFQGKRILVTGGGTGLGKEIAARYLELGAELWIAGRRGG
                     VLEETSKELVSRYGGVVKTHPIDIRDAAAVDAMVQRIWDESGPLTGLVNNAAGNFISP
                     TKDLTP"
BASE COUNT          496 a         1162 c         1165 g          581 t
ORIGIN      
        1 aggcgcaccg cctgatggaa acctcccagc acatcggcaa gctggtcctc accgtttagg
       61 ggaattcggg gtcagggtcg caattcagtt ttcacatacg taaaaacgaa ttgcgaccct
      121 gaccccgaat tcctaccgtt tagccgcttc gcgtttcagc cactcgacga acgcggcgac
      181 ctccggccgg tcggccgcgt gcctcgacac gacggcgtag tagccgcgcg ccgggtcggc
      241 ctggctcttg aagggcgcca ccagctcgcc cgaggcgagc aggtctttca ccagcgggct
      301 gcgcccaagc cccacgccat gcccggccac cgcggccggg atgatctgct cgaagcccga
      361 gaacgagagc gtgccggcgg gccgcaggtc ggcgatgcgc tcgacctcga gccaggtctt
      421 ccagtgcagc cacggatggc gcgcgtccgg gtcgtcgtag tgcagcagga tgtggtggcg
      481 caggtccgcc ggcttgtcga gcggtagcct cttcagcagc ttcgggctgg ccaccgggaa
      541 caccttctcg ccgaacaggc gcaccgcgtc cggcccggcc agcgccgccg gcccgtggcg
      601 cagcgcgatg tccagccctt cgctctcgag gtcctgcacc ttggtctcgg cggtgatgcg
      661 cacgtcgatg ccgggatggt cgcgcgtgaa gccggcgagc cgcgggatca gccatagcga
      721 ggcgaaggag ttcgtggtgg tgaccgagag cggccggcgc cggccggcct gcgagcgcag
      781 gcgctcggtg gccgtgcgca tggtcgaaat cacctgcgac gcggcggcgt agaactgctg
      841 gcccggcgcg gtcagcgtga gcgcgcggtg gcggcgctgg aagaggggca cgcccagctg
      901 gtcctccaac tccttgatct ggcggctcac cgcagactgc gtgaggtaga gctcttcgcc
      961 ggccttggtg aaggacaggt ggcgcgcggc ggcctcgaag ccgaccagca ggtcgagggc
     1021 cggtaacgca ttcctattcc tcatgcatgt cctgagtatt taccgtttga gccactcggc
     1081 ggaaggatgc atcctgcgca tgcgaaaagc aaggagacgc ccatgaaaat cgagctcgaa
     1141 gccgctgcag tcaggctgac cccgaaccag accctcaagc tgcgcgacag cgccggcagc
     1201 acggtgcacg cggtggaagg ctcggtgtgg atcaccgagg aaaaccagcc gcgcgacgtg
     1261 gtcctcgggc cgggcggctg ctaccgcctg aagcacgccg gcctcgccat catcaacgcg
     1321 ctcggcggcg gtgccgccgt gtcgttttcc tgaggagata ccgatgagac ccgacctggc
     1381 agaactccac gccgcagcgc gccgccagcg cgccatcgag atccaccggc tgattttcct
     1441 ccccatcgcg gctttttttc gccggccgag caagaaagaa caactgcggg tcgcgatatg
     1501 ccactagtga ggatctccgc cctgcagggg ccggagggct tcggccgcaa ggtcggccag
     1561 gtggtgtacc gcaccatggt cgatactatc gacgtgccgc gcgacgacaa cttccaggtg
     1621 atcaccgagc acccgcgcga cacgctggtg ttcgaccgca cctacatggg catcgcgcgc
     1681 tcggacggct tcgtctcgat ccagatcacg ctgagcgaag gccgcagcgt ggcgaagaag
     1741 cgccttttct accgcacgct ggccgagcgc ctgcagcgcg agctcaacgt gcggccggaa
     1801 gacgtgttcg tcaacctggt cgaggtcaag aaggaaaact ggtcgttcgg caacggcgtc
     1861 gcctcctacg cgcccgcgga aagcgtagcg gcctgaggtg ccgtccttcg gtgcgcgcgc
     1921 cgggctgatc gcgctctgct tcggcaattt cgtcatcggc accgggacgc tgatcgttcc
     1981 cggcatgctg ccgcagctcg ctgaagggct cggcgtgtcc ctgccgatcg ccggccagct
     2041 ggtgaccgcg ttcgccgccg ccgtgtgcct gggcgcgccg ctgctcgcgg gtgtcaccag
     2101 ccgcctcgac cggcgtgcgc tgctcgtcgc aatgcagctc gtgttcgtcg ccggccatct
     2161 cgctgccgcg ctggtctcgt cgttctggcc gatgctcgcc gttcgcgtcc tcacctcggt
     2221 gggcgcagcg ctgttcactg cgcaagccgc ctcggccgcg gcgctcctgg tgccggccga
     2281 agcgcgcggc cgcgccatcg ccttcgtctt cctcggctgg tcgatcgctt cggtcgcggg
     2341 cctgccgctc ggctcgtacg tctcggcggt gtggggctgg cgcgcgggat tcggcctggt
     2401 cgcggcgggc gcggccttcg gcgcggcggc agtgtggctg ctcctgcccc gcggactcag
     2461 agtggagccg gtgacctctg cgatgtggcg atcgatcctg cgcgaccccg ccctgatggc
     2521 gagcgtcggc gtgaccgcgc tttttgccgg cgccggcttc gcgctcttcg cctacttcgt
     2581 tcccgcggcg agaagctacc tcggcgcgtc gcccgagctg gtcagcggcc tgctggcggt
     2641 attcggcatc gcgggtctcg cgggcaacat gctcgcggtg cgctacatgg accgtctcgg
     2701 tgccgccaac gtagtgatgc tatgcctgct ttcgatgctc gctgcgcagc tgctttggcc
     2761 gtggtcgcag ggctcggtga cgctggtggc cctggtgatg atcggctgcg ggctgggtgt
     2821 gttcgcctgc aacagcgcgc agcagacgcg gcttgccgcg atcgcacccg cggccgcgtc
     2881 ggtatcgatc gcgctcaact cctcggcgat ctacctcggc caggccctcg gcccggcggc
     2941 cggcggggtc ctgatcgcgc acgtgcccgg caacgaaggc tatgccttga tcgcggcgat
     3001 cggcgtgccg ctgctcctcg ccgcgatcgg cttgtcgtac ttcgcatccc ttagaatgcg
     3061 cgctcgacct aggggcgtgc atgttcaagg aaggcctgtt ccagggtaag cgcatcctcg
     3121 tcaccggtgg cggcaccggc ctgggcaagg agatcgccgc acgctacctc gagctcggcg
     3181 ccgagctgtg gattgccggc cggcgcggcg gggttctcga agaaacatcc aaagaactcg
     3241 tttccaggta cggtggagtt gtaaaaaccc atcccatcga catccgcgac gcggcggcgg
     3301 tcgacgcgat ggtgcagcgc atctgggacg agagcgggcc gctcaccggc ctggtcaaca
     3361 atgccgccgg caatttcatc agcccgacga aggacctgac gccc
//
LOCUS       DASYJA010000518         2902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_805406, whole genome shotgun sequence.
ACCESSION   DASYJA010000518 DASYJA010000000
VERSION     DASYJA010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805406"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..346
                     /locus_tag="VGO02_11200"
     CDS             17..346
                     /locus_tag="VGO02_11200"
                     /inference="COORDINATES: protein motif:HMM:NF018684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HEV7478288.1"
                     /translation="MLRLALLLSLWMFSVAHACEEPDEERDLWGSYERAWAATDEDRR
                     ELLARAQGAWLDYREASCEMFASRDGLPQPQAYRQCRSFMAGERMFELRLICRTSSES
                     ENCDSVP"
     gene            380..1018
                     /locus_tag="VGO02_11205"
     CDS             380..1018
                     /locus_tag="VGO02_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020425009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV7478289.1"
                     /translation="MSQPPGQTKGERTRAAILDEALRIVSRAGLEGLTIGTLAEATGM
                     SKSGLFAHFGSREELLLAVLAHGQVEFTEVVFKPAIDHPRGIPRLRAMFVNWLDWTES
                     AELPGGCPMIGGASEFDDKPGPVRDMLAGGQRTWIETLKRGVRQGIEEGQLVADTDPE
                     QIAFEMFGIALVVHHHRRLLGYPKARSRALAALESLFERHAAAAKPLRAVSR"
     gene            1035..1934
                     /locus_tag="VGO02_11210"
     CDS             1035..1934
                     /locus_tag="VGO02_11210"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV7478290.1"
                     /translation="MPLSAALPQISTNVRFAASRLLFPELAGELAERLFLTPPRPRDA
                     AATALDLIDARSSIVEHKGRSIVTWRWGSRDAPAVLLAHGWGGSAAQMRAFVFPLLSA
                     GYRVIAFDQPAHGVSEGRLTGLPDFADVLAEVAWHHGEVEGVIAHSMGGAGAALAIAR
                     GLRVRRVVTIGASLDVSFFSRQFARWYWIPRRVLAAMQAAVEERSGVRWADLDMAKLA
                     PRLCAPALVIHDRNDRLVPWHKGAGFARGWPGARFFSTRGLGHRRILQDERVTSLAAE
                     FIAGHSVVASPAVPALPHPAPLY"
     gene            1938..2435
                     /locus_tag="VGO02_11215"
     CDS             1938..2435
                     /locus_tag="VGO02_11215"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV7478291.1"
                     /translation="MTAETRRSVTIEWSDPLPLAEAGRSMSGIEFLRAIRDRRLPAPP
                     IARLLGFDLVEVDTGYAVFEVVPAELHYNPIGVVHGGLAMTLLDSAMGCAVQTHMPAG
                     SGYTTLEAKTNLVRAITADTGKLRAIGRTLHVGKRMATAEGRLEDAAGKLYAHSSTTC
                     IVLSG"
     gene            2515..>2902
                     /locus_tag="VGO02_11220"
     CDS             2515..>2902
                     /locus_tag="VGO02_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478292.1"
                     /translation="MNFIWMPGEFLSGDPHAWREESRSLLLGGELNVPRDTAELLTSE
                     PGRYFSRNERNGLYYSKFGIANSLLALPPLWVERALGGDIGQRGHVPSLLLNNFWNLV
                     FCVALAAVLYALAAIYTARIALRVLFV"
BASE COUNT          418 a         1038 c          971 g          475 t
ORIGIN      
        1 aagagaggag cgctcaatgc tgcgcctggc cctgctgtta tctctatgga tgttttcggt
       61 tgcgcacgcc tgcgaggaac cggacgagga acgggatctg tggggtagtt acgagagggc
      121 atgggccgcc accgacgaag accgaagaga actcctcgcc agggcccagg gcgcgtggct
      181 cgactaccgc gaagcgagct gcgagatgtt tgccagccgc gacggcttgc cacagccgca
      241 agcgtaccgc cagtgccgca gcttcatggc cggcgagcgg atgttcgagc tgcgcctcat
      301 ctgccggacc agcagcgaaa gcgagaactg cgactcggtg ccttgacatt ttagaacggt
      361 cgtactattc tgtgcgccga tgagccagcc ccccggccag accaaaggcg agcgcacccg
      421 tgccgcgatc ctcgacgagg cgctgaggat cgtcagccgc gcgggccttg aaggcctgac
      481 catcggcacc ctggccgagg cgaccggcat gtcgaagtcg ggcctgttcg cccacttcgg
      541 ctcgcgcgag gagctgctgc tcgcggtgct ggcgcatggc caggtcgaat tcaccgaggt
      601 agtgttcaag ccggcgatcg accatccgcg cggcataccg cgcctgcgcg ccatgttcgt
      661 caactggctc gactggaccg aatcggccga gctgcccggc ggctgcccca tgatcggcgg
      721 cgccagcgaa ttcgacgaca agccggggcc ggtgcgcgac atgctcgccg gcggccagcg
      781 cacctggatc gagacgctga agcgcggcgt gcgccagggg atcgaggaag gccagctggt
      841 cgccgacacc gacccggagc agatcgcctt cgaaatgttc ggcatcgcgt tggtggtgca
      901 ccaccaccgg cgcctgctcg gctacccaaa ggcgcgctcg cgcgccctcg ccgcgctcga
      961 gtcgctgttc gagcgccacg ccgccgccgc caaaccgctg cgcgcagtct cccgctagac
     1021 aaggaagcaa cgccatgccg ctctccgccg ccctgccaca aattagcacg aacgttcgct
     1081 ttgccgcttc gcggctgctc ttcccggagc tcgccggcga gcttgccgag cgcctgttcc
     1141 tgacgccgcc gcgcccgcgc gatgccgctg ctaccgcgct cgacctgatc gatgcgcgct
     1201 cgagcatcgt cgagcacaag ggccgctcga tcgtcacatg gcgctggggc tcgcgcgacg
     1261 cgccggccgt gctgcttgcc catggctggg gtggctccgc tgcgcagatg cgcgccttcg
     1321 tctttccgct gctgtcggcg ggctaccgcg tcatcgcctt cgaccagccg gcgcacggcg
     1381 tgtcggaagg caggctcacc gggctgcccg atttcgccga cgtgctggcg gaggtcgcct
     1441 ggcaccacgg cgaggtagag ggcgtgatcg cgcactcgat gggcggcgcc ggcgccgcgc
     1501 tcgccatcgc ccgcggcctg cgcgtgcggc gcgtcgtgac catcggcgcc tcgctcgacg
     1561 tctcgttctt ttcgcgccag ttcgcgcgct ggtactggat tccgcgccgc gtcctcgcgg
     1621 cgatgcaggc ggcggtcgag gagcgctccg gcgtgcgctg ggcggacctc gacatggcca
     1681 agctcgcgcc gcggctctgc gctcccgctc tggtgatcca cgaccgcaac gaccggctgg
     1741 tgccctggca caagggcgcg ggttttgcgc gcggctggcc cggcgcgcgc ttcttcagca
     1801 cgcgcggtct cggccatcgc cgcatcctgc aagatgagcg cgtcacctcg ctcgccgccg
     1861 aattcatcgc cggccactcg gtcgtggcca gtccagccgt tccggcgctg ccgcatcctg
     1921 cgcccctgta ctgacccatg acggctgaaa ccaggcgtag cgtcaccatc gagtggagcg
     1981 acccgctacc gctcgccgag gccggccgct cgatgtcggg catcgaattc ctgcgcgcca
     2041 tccgcgaccg caggctgccg gcgccgccga tcgcgcggtt gctcggcttc gacctggtcg
     2101 aggtcgacac gggctacgcg gtgttcgagg tagtgcccgc cgagctgcac tacaacccga
     2161 tcggcgtggt gcacggcgga cttgccatga cgctgctcga ttcggcgatg ggctgcgcgg
     2221 tgcagacgca catgccggcc ggctccggct acaccacgct cgaggcgaag accaacctgg
     2281 tgcgcgccat caccgccgac accggcaagc tgcgcgccat cggcaggacg ctgcacgtcg
     2341 gcaagcgcat ggcgaccgcg gagggtcgcc tcgaggacgc tgcgggcaag ctctatgcgc
     2401 attcctccac gacctgcatc gtgctgtccg gctaagatag gatttttcca gcccgaggcg
     2461 cgcatcgcca caagccgcat cgtcctcctc gccctgctgc tcggggtcgc tgcggtgaac
     2521 ttcatctgga tgccgggcga gttcctctcc ggcgacccgc acgcctggcg cgaggagagc
     2581 cgcagcctgc tgctgggcgg cgagctgaac gtgccgcgcg acaccgcgga gcttctcacc
     2641 agcgagcccg gcagatattt ttcccggaac gagcgcaacg gcctgtacta ctcgaagttc
     2701 ggcatcgcca actcgctgct cgcgctgccg ccgctgtggg tcgagcgcgc gctcggcggc
     2761 gacatcggac agcgcggcca cgttccgagc ctgctgctca acaatttctg gaacctcgtg
     2821 ttctgcgtgg cgctcgccgc ggttctctat gcgcttgcgg cgatctatac cgcgcgcatc
     2881 gcgctgcgcg tgctgttcgt cg
//
LOCUS       DASYJA010000519         1663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1895448, whole genome shotgun sequence.
ACCESSION   DASYJA010000519 DASYJA010000000
VERSION     DASYJA010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1895448"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..467
                     /locus_tag="VGO02_11225"
     CDS             138..467
                     /locus_tag="VGO02_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478293.1"
                     /translation="MNYVPAVMVLAALLVAPTGQAANPTVDALAAAPANVFLGEARRS
                     TGKSFLDHVRTTYAEESVYSVPATFPGPLVAAPRRQPDTPWMLLACFGLLLYLGCRRG
                     KALGAAF"
     gene            490..1152
                     /locus_tag="VGO02_11230"
     CDS             490..1152
                     /locus_tag="VGO02_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478294.1"
                     /translation="MLAGCSVSEGGTPPSAAARVNAEVISVAELEAARKRGDSSGGDT
                     LERLIDQRLARQHALEQGLERSPQIAQALESAKTDILARAYRQLVAEAQARPTPAEIG
                     SYYAAHPELFAKRRIYSLESLDARFGVNRSTLAAEQLPLELVSRLQAMAEGDVLAIDD
                     GAGGAVVVRIVAARLAPLDQASAAPLIEKFLLAQRSSEALAAEMKRLRATARIEYPAG
                     VK"
     gene            1158..>1663
                     /locus_tag="VGO02_11235"
     CDS             1158..>1663
                     /locus_tag="VGO02_11235"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEV7478295.1"
                     /translation="MLQVLLLVGTLPFAFAQDNDRLAQYRLGAGDSIRISVFQNPNLT
                     LETRVGEDGAITYPLIGRMALGGTTIAEAERSITRALDSGNFIREPQVTILLLQTRSA
                     QVSVLGLVNRAGRYPLDTVNVRVSEMIAIAGGVATAGGDTAILTGERAGKSFRREIDI
                     AKLFSENN"
BASE COUNT          266 a          583 c          549 g          265 t
ORIGIN      
        1 gtgcgtaaga gcgctttgga gccaacgctg tcgctgcgca tcgacgtcgc cgttaacgtg
       61 tagttgcttc tcgtctttct tagcacctaa gagccatagg cgcccctgag cgagcccact
      121 acgatgcctt cgcgctagtg aactacgtgc ctgcagttat ggtcctggcg gcgctgctgg
      181 ttgcgcctac agggcaagcg gcgaacccca cggtcgatgc actcgccgcg gcgccggcga
      241 acgtgttcct cggcgaggca cgccgcagca ccggcaagtc cttcctggac catgtaagga
      301 ccacttacgc tgaggagtcc gtttattctg ttcctgcgac cttccccgga ccgctcgtcg
      361 cggcgccacg gcgccagccc gacaccccgt ggatgctgct ggcgtgcttc ggtctgctcc
      421 tgtacctggg ctgccggcgc ggcaaagccc tgggcgctgc gttctgaggc gctgcttctc
      481 tgcagcgccg tgctcgctgg ctgcagcgta agcgagggcg gaacgccgcc cagcgccgcc
      541 gcgcgcgtca acgccgaggt gatcagcgtc gctgagctcg aagccgcccg aaaaaggggc
      601 gatagctccg gcggcgacac gctcgagcgg ctgatcgacc agcgcctcgc gcgccagcac
      661 gcgctcgagc agggcctcga gcgctcgccg cagatcgcgc aggcgctcga gtcggcgaag
      721 accgacatcc tcgcgcgcgc ctatcgtcag ctcgtcgccg aagcgcaggc gcggccgacg
      781 ccggcggaga tcggcagcta ctacgccgcg caccccgagc tcttcgccaa gcggcgcatc
      841 tacagcctgg aatcgctgga tgcgcgcttc ggcgtcaatc gctcgacgct cgccgcggag
      901 cagctgccgc tcgagctcgt gtcgcggctg caggcgatgg cggaaggcga cgtcctcgcc
      961 atcgacgacg gcgccggtgg cgcagtggtg gtgcgcatcg tcgcggcgcg cctcgcgccg
     1021 ctcgaccagg cgagcgccgc gccgctgatc gagaagttcc tgctcgcgca gcgctcgagc
     1081 gaggcgctcg ccgcagaaat gaagcgcctg cgcgcgacgg cgcgcatcga atacccggcg
     1141 ggggtcaaat gaggcgattg ctgcaggttc tgctgctggt tggcacactg ccgtttgcgt
     1201 tcgcacaaga caacgacagg cttgcgcagt accgcctggg cgccggcgac tcgatccgca
     1261 tctcggtgtt ccagaacccg aacctcacgc tcgagacgcg ggtgggcgag gacggcgcga
     1321 tcacctatcc cctgatcggc cgcatggcgc tcggcggcac caccatcgcc gaagccgagc
     1381 gctcgatcac gcgcgcgctc gacagcggca acttcatccg cgagccgcag gtgaccattc
     1441 tcctgctgca gacgcgcagc gcccaggtgt cggtgctcgg gctggtgaac cgcgccgggc
     1501 gctacccgct cgacaccgtc aacgtgcgcg tctccgagat gatcgccatc gcgggcggcg
     1561 tcgccaccgc gggcggcgac accgccatcc tcaccggcga gagagcaggc aagtcgttcc
     1621 gcagggaaat agatattgca aaactttttt ctgaaaataa tca
//
LOCUS       DASYJA010000520         4913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_805471, whole genome shotgun sequence.
ACCESSION   DASYJA010000520 DASYJA010000000
VERSION     DASYJA010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805471"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VGO02_11240"
     CDS             complement(<1..507)
                     /locus_tag="VGO02_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7478296.1"
                     /translation="MAYGRGPAAVRAIEQVSLDIAQGEFIAVVGPSGCGKSSLMRLIS
                     GLHPPASGNLSLEGKPIAGPLKSVGMAFQNANLLPWRNALENVLLPLEIVEPYRSGFS
                     KQKYAPKAKSLLESVGLQGFAEKYPWELSGGMQQRTSICRALIHEPEMLLLDEPFGAL
                     DAFTREELW"
     gene            complement(533..1537)
                     /locus_tag="VGO02_11245"
     CDS             complement(533..1537)
                     /locus_tag="VGO02_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7478297.1"
                     /translation="MRLVALLTAMLVSGAALAQKEIPIRFALDWRFEGPAAPFFVAID
                     KGYYKAEGLNVTIDPGAGSVEGINRVASGAYEVGFADINSLVKYRDNPRNLPVKAVMM
                     VYDTPAFSIVSLKKNGIAKPKDLEGKILGAPAADGAYAQWPIFVQANGIDATKVRIEN
                     IGFPVREPMLAQGKVDAIAGFWFSSYMNLKANGVPGDDIVVLLMRDFGVDLYGNVIIA
                     NPDLMRLQPKAVAGFVKATIRGIQETIRSPDTAIDSLMSRNPIAKRDVELERLKLSLE
                     KNFVTPDVRKNGLGAVDAKRLERSIDQIGLTFQYSNKPKAADIFTAQYLPPKEQRLVK
                     "
     gene            complement(1605..2765)
                     /locus_tag="VGO02_11250"
     CDS             complement(1605..2765)
                     /locus_tag="VGO02_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEV7478298.1"
                     /translation="METYLLDWLNLLVRWAHVIVGIAWIGASFYFIWLDDRLEASPDP
                     QVAGELWAIHGGGFYSARKYRLAPAEVPATLHWFKWEAYWTWITGFALLVLMYYVNAE
                     LYLIDPAVMALSKPAAIGLALGSLILGLIAYEAVCRSPLGKNEALLSAVIFLLLALAA
                     WGLAQVFSGRGAFIHYGAILGTIMAANVAHVIIPGQRRMVQAMREGREPDAKDSLIGK
                     QRSVHNTYFTLPVVFVMISNHYASVFGHRWGWLALVALTLAGALIRVWFVLRHKASAS
                     SWVWILGVLCVLLAAALVAPPRPTVASKVDFSEIRKIVDTRCVACHAEKPSFQGMNEA
                     PKGVKLDTDERIRAQAAPIHQQTVLGKAMPPGNLTAMTQEERQLLDRWYRAR"
     gene            complement(2769..3101)
                     /locus_tag="VGO02_11255"
     CDS             complement(2769..3101)
                     /locus_tag="VGO02_11255"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF012785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEV7478299.1"
                     /translation="MPGLSIHVVDISRGVVALGMRVEVLFKERKVLSGVTSAKGALEE
                     SATLEPGEYEAVFHVAEWYRKEKVALPAVPFLDVVTYTFGISDPAQHYHLPFKCTPWG
                     YSCFRGGA"
     gene            complement(3102..4061)
                     /locus_tag="VGO02_11260"
     CDS             complement(3102..4061)
                     /locus_tag="VGO02_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7478300.1"
                     /translation="MILRSILAAILLCCGSAAAQTWPTKPIRVVVTFSPGGSSDIVAR
                     LIAIPLQAELGQSVIVDNKPGAGGTIGALEAARAAPDGYTLLLSNSAPISISPAMQDK
                     ASYDPLKAFMHVSYIGSVANVFVVHPSVPAKNMAELVAWIKAEPNPVNYGSGGIGSIG
                     HLLGETMKKDLGLRLEHVGYKGSSPMHNDLLAGTIKLAIDTLPQNVPFMKDGKLRAIA
                     VTSPTRAPMAPEVPTVLELGQKKLVAENFLGISGPAGLPKSVVDRLQAAMKKSLANPT
                     VMQRLADLGVQGGDMTPEQFSAFVAAQVKDWYQPVKDSGAKLN"
     gene            complement(4065..4418)
                     /gene="uraH"
                     /locus_tag="VGO02_11265"
     CDS             complement(4065..4418)
                     /gene="uraH"
                     /locus_tag="VGO02_11265"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEV7478301.1"
                     /translation="MGRITTHVLDTANGRPGAGMKIEFSVFEGQGWKHLKTVNANKDG
                     RTDEPLAEGAALKPGQYRIVFHVADYFRSQGAKQSDPPFLDRVPIRFGIEDASAHYHV
                     PLLCSPWGYTTYKGS"
     gene            complement(4418..>4913)
                     /gene="uraD"
                     /locus_tag="VGO02_11270"
     CDS             complement(4418..>4913)
                     /gene="uraD"
                     /locus_tag="VGO02_11270"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEV7478302.1"
                     /translation="VPGDKQAFVARFGGVFEHSPWVAERVWHKRPFASAEALHREMMH
                     AVRNARPAEILALVRAHPELAGREAKAGGLTADSSGEQGRLGFTSLGREEFVKMQELN
                     RAYREKFGFPCIVALRLHATRASVVAEMERRVGNNDETELRKALEQIGHITRGRLDKL
                     FGEE"
BASE COUNT          850 a         1584 c         1626 g          853 t
ORIGIN      
        1 ccacagctcc tcgcgcgtga aggcgtcgag cgcgccgaac ggctcgtcga gcagcagcat
       61 ctcgggctcg tggatcagcg cgcggcagat cgaggtgcgc tgctgcatgc cgcccgacag
      121 ttcccacggg tatttctcag cgaatccctg cagccctacg gactccagga gggacttcgc
      181 cttgggcgcg tatttctgct tcgagaagcc agaacggtaa ggctcgacga tttcaagcgg
      241 cagcagcacg ttctcgagcg cgttgcgcca cggcaggagg ttggcgttct ggaacgccat
      301 gccgacggac ttgagcggcc cagcaatggg tttcccctca agagacaggt tcccggacgc
      361 tggcggatgc aaccccgaga tgagcctcat cagcgagctt ttgccgcagc cggaagggcc
      421 gaccacggca atgaattcgc cctgcgcgat gtcgaggctg acctgctcga tggccctgac
      481 cgcggcgggg ccgcggccgt aggccatgga gacgttttct agcttgacca gactacttga
      541 cgaggcgctg ctccttgggc ggcaggtact gcgcggtgaa tatgtcggcg gccttcggct
      601 tgttcgagta ctggaaggtg aggccgatct gatcgatcga gcgctcgagg cgcttcgcgt
      661 ccaccgcgcc caggccgttc ttgcgcacgt cgggcgtgac gaagttcttc tccagcgaga
      721 gcttgaggcg ctcgagctcg acgtcgcgct tggcgatcgg gttgcggctc atgagcgagt
      781 cgatcgcggt gtcggggctg cggatcgtct cctggatgcc gcggatggtc gctttcacga
      841 agccggccac cgccttgggc tgcaggcgca tcaggtcggg gttggcgatg atcacgttgc
      901 cgtagaggtc gacgccgaag tcgcgcatca gcagcaccac gatgtcgtcg cccggcacgc
      961 cgttcgcctt gaggttcatg taggacgaga accagaagcc ggcgatggcg tcgaccttgc
     1021 cctgcgcgag catcggctcg cgcaccggga agccgatgtt ctcgatcctc actttggtag
     1081 cgtcgatccc gttcgcctgc acgaagatcg gccactgcgc atacgcgccg tcggccgccg
     1141 gcgcgccgag gatcttgcct tcgaggtcct tcggcttggc gatgccgttc ttcttcagcg
     1201 agacgatgga gaaggccggc gtgtcgtaca ccatcatcac cgccttcacc ggcaggttgc
     1261 gcgggttgtc gcggtacttc accagcgagt tgatgtcggc gaagccgact tcataggcgc
     1321 cggaggcgac gcggttgatg ccctcgaccg agcccgcgcc cgggtcgatg gtcacgttga
     1381 ggccctcggc cttgtagtag cccttgtcga tggcgacgaa gaacggcgcc gccggcccct
     1441 cgaagcgcca gtcgagcgcg aagcggatcg ggatctcttt ctgtgcaagc gcagcaccag
     1501 aaacgagcat cgcggtgagc aacgcgacca aacgcatgac gaatccccct gtctgcctac
     1561 gtggatgaag tctacagcac gacgcgtgcc agaagccggc agtgctagcg ggcgcgatac
     1621 cagcgatcga gcagctggcg ctcctcctgc gtcatcgccg taaggtttcc cggcggcatc
     1681 gccttgccga gcaccgtctg ctggtggatc ggcgccgcct gagcgcggat gcgctcgtcg
     1741 gtatcgagct tcacgccttt cggcgcttcg ttcatgccct ggaagctcgg cttctcggcg
     1801 tggcacgcaa cgcagcgggt atccacgatt tttcgtatct ccgagaaatc gaccttggag
     1861 gcgaccgtgg gccgcggcgg cgcaacgaga gcggccgcga gaagcacgca aagcaccccg
     1921 agaatccaga cccatgagga ggcgctcgcc ttgtggcgca gcacgaacca gacgcgtatc
     1981 agcgcgcccg ccagcgtgag cgcgacgagc gcgagccagc cccagcggtg gccgaagacg
     2041 ctggcgtagt gattcgagat catcacgaac accaccggca gcgtgaagta cgtgttgtgg
     2101 acgctgcgct gcttgccgat caggctgtcc ttcgcatcgg gctcgcgtcc ttcgcgcatc
     2161 gcctgcacca tgcgccgctg gccggggatg atgacgtgcg cgacgttcgc cgccatgatc
     2221 gtgccgagga tcgcgccgta gtggatgaag gcgccgcggc cgctgaacac ctgcgccagg
     2281 ccccacgcgg caagagccag aagaaggaag attactgcgc ttagaagagc ttcgttcttt
     2341 ccaagtggag agcggcacac cgcttcatat gcgatcaacc caagaatcag ggaacccaag
     2401 gccagaccga tcgccgccgg cttggacagc gccatcacgg ccggatcgat caggtacagc
     2461 tcggcgttga cgtagtacat cagcacaagc agcgcgaagc cggtgatcca ggtccagtac
     2521 gcctcccact tgaaccagtg gagggtcgcc ggaacctccg caggcgccag ccggtacttg
     2581 cgggcgctgt agaagccgcc gccgtggatc gcccacagct cgccggcgac ctgcgggtcg
     2641 ggcgaggcct cgagacgatc gtcgagccag atgaaataga acgaggcgcc gatccacgca
     2701 atgccgacga tcacgtgcgc ccagcgcacg agaaggttca gccaatcgag gagatacgtt
     2761 tccaactgct acgcgccgcc cctgaagcag gaatagcccc agggtgtgca cttgaacggc
     2821 aggtggtagt gctgcgcggg atccgagatg ccaaaggtgt aagtgacgac atcgaggaac
     2881 ggcaccgcgg gaagcgcgac cttctccttg cggtaccact ccgcgacatg gaacacggcc
     2941 tcgtactccc ccggctccag ggtcgcactc tcctcgagcg cgcctttcgc cgaggtcacg
     3001 ccgctgagga ctttccgctc cttgaaaagg acctcgacac gcatgcccaa ggcgacgacc
     3061 ccgcgcgaaa tgtccactac gtgtatggac aggcccggca cctagttcaa cttcgcgccg
     3121 gagtccttca ccggctggta ccagtccttg acctgcgcgg cgacgaaggc gctgaactgc
     3181 tccggcgtca tgtcgccgcc ctgcacgccg aggtcggcga ggcgctgcat gacggtcggg
     3241 ttggcgaggg acttcttcat tgccgcctgc aggcggtcga ctacgctttt gggcaatccg
     3301 gccgggccgg agatgccgag gaagttctcg gccaccagct tcttctggcc gagctcgagc
     3361 acagtgggca cctccggcgc catcggcgcg cgcgtcggcg acgtgacagc gatggcgcgc
     3421 agcttgccgt ccttcatgaa cggcacgttc tgcggcaggg tatcgatggc gagcttgatg
     3481 gtgccggcga ggaggtcgtt gtgcatcggg gacgagccct tgtagccgac gtgctccagc
     3541 ctcagcccga ggtccttctt catggtctcg ccgagcaggt ggccgatcga gccgatgccg
     3601 cccgagccgt agttgaccgg gttgggctcg gccttgatcc acgcgacgag ctcggccatg
     3661 ttcttggctg gcaccgacgg gtgcacgacg aagacgttgg cgaccgagcc gatgtagctc
     3721 acgtgcataa aggccttgag cgggtcgtag ctcgccttgt cctgcatcgc cggcgagatg
     3781 ctgatgggag cgctgttgga gagaagcagg gtatagccgt cgggagcggc gcgcgccgcc
     3841 tcgagcgcgc cgatggtgcc gccggcgccc ggcttgttat cgacgatcac cgactggccg
     3901 agctcggcct gcaggggaat ggcgatcagg cgggcgacga tgtcggacga gccgccgggc
     3961 gagaacgtga cgacgacccg gatcggcttg gtgggccagg tctgggcggc agcgctaccg
     4021 cagcaaagca ggatcgcggc gaggatggat ctgaggatca ttctttagct ccctttgtac
     4081 gttgtgtagc cccacggcga gcaaagcagc gggacatggt agtgagcgga agcatcctct
     4141 atgccaaagc ggatcggcac ccggtccagg aacgggggat cggattgctt cgctccctgg
     4201 gagcgaaagt agtcggcgac atgaaagacg atccggtact gcccgggctt gagcgccgcg
     4261 ccctcggcga gcggctcgtc ggtccggccg tccttgttgg cgttcaccgt cttcaggtgc
     4321 ttccacccct gcccttcgaa aacggagaac tcgatcttca tcccggcgcc aggccggccg
     4381 ttggccgtgt cgagcacgtg ggtcgtgatc ctgcccatca ttcctcccca aacagcttgt
     4441 cgagccggcc gcgcgtgatg tggccgatct gctcgagcgc cttgcgcagc tccgtttcgt
     4501 cattattgcc gacccggcgc tccatttcag ccactacgct cgcgcgggtc gcatgcaagc
     4561 gtagcgcgac gatgcatggg aagccgaact tctcgcggta ggcgcggttg agctcctgca
     4621 tcttcacgaa ctcctccctg ccgagagaag tgaagcctag gcgcccctgc tcgccggacg
     4681 aatccgcggt caggccgccc gccttcgcct cgcgcccggc gagctccgga tgcgcgcgaa
     4741 ccagcgccag gatctcggcg ggcctcgcgt tgcgcacggc gtgcatcatc tcccggtgca
     4801 gggcctcggc agaagcgaac ggccttttgt gccaaacccg ttcggccacc cacggtgaat
     4861 gctcgaatac gccgccgaag cgcgcgacga acgcctgctt gtctcccggg aca
//
LOCUS       DASYJA010000521         2960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2438851, whole genome shotgun sequence.
ACCESSION   DASYJA010000521 DASYJA010000000
VERSION     DASYJA010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2438851"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1399)
                     /gene="glgX"
                     /locus_tag="VGO02_11275"
     CDS             complement(<1..1399)
                     /gene="glgX"
                     /locus_tag="VGO02_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEV7478303.1"
                     /translation="MNAPEIKLSRVREGLPYPKGATWDGKGVNFSLFSAHATKVELCV
                     FSNDGKTEKERIELPEYRDEIWHGYVPDLPPGTIYGYRVHGPYAPEAGHRFNPNKLLL
                     DPYARAHIGALEWNPAVFGYKVGDAAADLSFDERDSAPFMPKCVVVDPDFDWKREKVW
                     RPVSWDRTIVYEAHVRGLTKLHPGVPEHERGTFCGFAAKPIIDHIKSLGVTTVELLPV
                     HTFINDEFLLKRNLTNYWGYNSIGFFAPDPRYAADPAKTLQEFKEMVARFHDAGLEVI
                     LDVVYNHTAEGNELGATLSFKGIDNASYYRLAENKRHYVNDTGTGNTLNLSHPRVIQM
                     VTDSLRYWVQDMHVDGFRFDLGTILAREPGGFETHNGFMKAVIQDPVLGAVKMIAEPW
                     DCGPGGYQLGRFPPGWGEWNDQYRDTVRDYWRGEAGAAQLAPRLCASPDVFNNHGRQP
                     WASVNFITAHDGFTLN"
     gene            complement(1625..2833)
                     /locus_tag="VGO02_11280"
     CDS             complement(1625..2833)
                     /locus_tag="VGO02_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7478304.1"
                     /translation="MERSWRTPFVVLACGCLIMTISFGIRAGFGLFLQPMALEYGWGR
                     EVFSFSIALQNLMWGALGAVAGGFADRYGPGRVIAGGAICYILGLIGLAYIATPLLMH
                     LNCGLLLGGALGGTSFGIILVVIGRTVAPERRSLYMGVATAAGSFGQFALVPLTQTLI
                     AALDWHHALLVLAGIAALIVPLTLGLAGKPAAAAAAKPQSIGEALREAMGEKGFHLLF
                     WGYFVCGFHIAMLTVHLPAFVTDAGLKPSHGMTALALIGLFNIVGTLGAGYLGGRFSK
                     KYLLSAIYTVRAALISLLVFFPLSPLILYVFACGIGLLWLGTVPLTNGLVGQIFGMRY
                     AAMLASVVFFGHQIGSFIGVWLAGYLYDHTGSYSGAFLASIGLGIFAAIVNLPVNEKP
                     LAERRVAAAA"
BASE COUNT          543 a          917 c         1026 g          474 t
ORIGIN      
        1 cgttcaaggt gaagccgtcg tgcgcggtga tgaaattgac gctcgcccac ggctggcggc
       61 catggttatt gaagacatcc ggcgaagcgc agagccgcgg cgcgagctgg gccgcgcctg
      121 cttctcccct ccagtagtcg cgcacggtgt cgcggtactg gtcgttccac tcgccccagc
      181 ccggcggaaa gcgcccgagc tggtagccgc cggggccgca gtcccagggc tcggcgatca
      241 tcttcaccgc gccgagcacc ggatcctgga tgaccgcctt catgaagccg ttgtgcgtct
      301 cgaagccgcc cggctcgcgc gccaggatgg tgcccaggtc gaagcggaag ccgtcgacgt
      361 gcatgtcctg cacccagtag cgcaggctgt cggtcaccat ctggatgacg cgcgggtggc
      421 tgaggttgag cgtgttcccg gtaccggtgt cgttgacgta gtgccgcttg ttttccgcca
      481 gccggtaata ggacgcgttg tcgatgccct tgaaggagag cgtggcgccg agctcgttgc
      541 cctcggcggt gtggttgtag accacgtcga ggatcacctc cagcccggcg tcgtggaagc
      601 gcgccaccat ctccttgaac tcctgcagcg tcttcgccgg gtcggcggca tagcgcgggt
      661 cgggagcgaa gaagccgatg ctgttgtagc cccagtaatt ggtcagattt cttttcagca
      721 ggaattcatc attgatgaac gtgtgaacgg gaagcagctc gaccgtggtg acgccgaggg
      781 acttgatgtg gtcgatgatc ggcttcgcgg caaagccgca gaacgtgccg cgctcgtgct
      841 cgggcacgcc ggggtggagc ttggtgaggc ccctgacgtg ggcctcgtag acgatggtgc
      901 gatcccacga caccggccgc cagaccttct cgcgcttcca gtcgaagtcc gggtcgacca
      961 ccacgcattt cggcatgaag ggcgcgctgt cgcgctcgtc gaaggacaga tccgccgcgg
     1021 cgtcgcccac cttgtagccg aatacggccg ggttccactc gagcgcgccg atgtgcgcgc
     1081 gcgcataggg atcgagcagc agcttgttcg ggttgaagcg gtgcccggct tccggcgcgt
     1141 aggggccgtg cacgcggtag ccgtagatgg ttcccggcgg caggtcgggc acataaccgt
     1201 gccagatctc gtcgcggtac tcgggcagct ctatgcgctc tttttcggtc tttccgtcat
     1261 tactgaaaac acaaagctcc actttcgtgg cgtgcgcgga gaagagcgag aaattgacgc
     1321 ccttcccatc ccaggtagcg cctttcgggt acggcagacc ctccctgacg cgggaaagct
     1381 tgatttcggg ggcgttcatg gcatttggac agcaacacta gggccgtgtc aggagcggcc
     1441 ggtggcgccg cgcctgtgcc gttcggacta tgggcgcgat ccgcgccggc tgctcaggag
     1501 cagaggacga ggttagcgaa ctcgatcagc atgttcggct tgaggtaggc cgagaacgcg
     1561 gcggcggcgg tgaaggccag cagacctacg gcagtgcaca taagaacaaa tttctttctg
     1621 gaggtcatgc ggcggcggca accctgcgct cggccagcgg cttctcgttc accggcagat
     1681 tgacgatggc ggcgaagatc cccaggccga tcgaggcgag gaaggcgccg ctgtacgagc
     1741 cggtgtggtc gtacaggtag ccggcgagcc agacgccgat gaagctgccg atctggtggc
     1801 cgaagaacac gaccgacgcg agcatcgccg cgtagcgcat gccgaagatc tggccgacca
     1861 ggccgttggt gagcggcacg gtgccgagcc acagcaggcc gatgccgcaa gcgaacacat
     1921 aaaggataag gggagacagc gggaaaaaga ccagcagcga gatcagcgcg gcacgcacgg
     1981 tgtagatcgc cgacagcagg tacttcttgc tgaagcgccc gccgagatag cccgcgccga
     2041 gcgtgccgac gatgttgaac agcccgatca gcgcgagggc ggtcataccg tgcgatggct
     2101 tgaggccggc gtcggtgacg aaggccggca ggtgcaccgt cagcatggcg atatggaagc
     2161 cgcagacgaa gtagccccag aagaggaggt ggaagccctt ctcgcccatc gcctcgcgca
     2221 gcgcttcgcc gatcgactgc ggtttcgcgg cggcagccgc ggccggcttg ccggcgaggc
     2281 ccagcgtaag cggcacgatc agcgccgcga tgcccgcgag cacgaggagc gcgtggtgcc
     2341 agtctagcgc cgcgatcagg gtctgcgtga gcggcaccag cgcgaactgg ccgaacgagc
     2401 cggcggcggt cgcgacgccc atatacaggc tgcgccgctc cggcgcaacg gtgcggccga
     2461 tcaccaccag gatgatgccg aacgaggtgc cgccgagcgc gccgccgagc agcaggccgc
     2521 agttgaggtg catgagcagc ggcgtggcga tgtaggcgag gccgatcagg ccgaggatgt
     2581 agcagatcgc gccgccggcg atcacgcgcc cgggaccgta gcggtcggcg aagccgccgg
     2641 ccaccgcgcc gagcgcgccc cacatcaggt tctgcagcgc gatcgagaag gagaatactt
     2701 ccctgcccca gccgtactcg agggccatcg gctgcaggaa caggccgaag ccggcgcgga
     2761 tgccgaacga gatcgtcatg atcaggcagc cgcaggcgag gacgacgaac ggggtgcgcc
     2821 aggatctttc cacaggccgc agtctagcct agggccaacc ttgtcttccc cgtgcatttc
     2881 gcatgcgacg gattcccttc actcatccca agaaatagcg cgggatccag ttggaaagcc
     2941 cgggaatgaa aatcaccagg
//
LOCUS       DASYJA010000522         3968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1803533, whole genome shotgun sequence.
ACCESSION   DASYJA010000522 DASYJA010000000
VERSION     DASYJA010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1803533"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VGO02_11285"
     CDS             <1..641
                     /locus_tag="VGO02_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEV7478305.1"
                     /translation="AIEGPDADDPYSRSGAPVQFSPQQFKFAVPSQLEFHGDSGYAKL
                     FAQAILKLRQIGGTPVEIDFAPFLEAGALLYGPWVAERAAHLEPHMKDMLPVVREILE
                     GAKKYSAVDFFKARNRLDELKQKSRETFKQADVIFVPTTPTIYQLAQVEKKPVELNNH
                     LGHYTSFTNLLDLAAVSLPAGMRQDGVPFGVSLIAPAFTDKALLALGARFQG"
     gene            complement(649..2262)
                     /locus_tag="VGO02_11290"
     CDS             complement(649..2262)
                     /locus_tag="VGO02_11290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEV7478306.1"
                     /translation="MKSNEPNSRRSFLKGAAATPLLIAGSAALVPKPAQASGTAVGPS
                     TTTEPYLRPTIAGVKLVSILTVGDSVGGYRMVGIPDGLGAFDSGHREFTLLMNHELTA
                     NPPGIARAHGASGAFVSRWTIDSETLRVKKGQDHTPSPGKVFVWDAAAGRHVAYVPTV
                     PPSVLWQRHCSADLPAPGAFFHRGRGTTERIYMNGEEVTDGRAWARVATGTHMGEAWQ
                     LPRFGRMAYENAVASPYSQDKTLVALTDDSSAATGPLPNNGSEVYFYIGTKQSGGTPI
                     EQAGLMNGLLYGVSISVEGAHVNGEDNAFGLGNSTSGYIGSGRFELVSLGDTSGITTG
                     LALESLSNASNVTRLQRCEDGAWDPREGHENDFYFVTTASITTNCRLWRLRFDDIERP
                     EAGGTVQIMLRGDEGHRMLDNVTIDRLGRILMDEDPGGDNRVAKIWLYAIDTRELIQL
                     AAHTPKFFDPDSKQPEFITIDEESSGIVDAAHILGEGWFLVDVQSHKPSTDPELVEGG
                     QLLALWVDPDIGFNGDEKRHRHDHNEGRR"
     gene            complement(2384..3367)
                     /locus_tag="VGO02_11295"
     CDS             complement(2384..3367)
                     /locus_tag="VGO02_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV7478307.1"
                     /translation="MLRLSLACWDYDRTRALADGRVQPEGIDLIYLNQPVEETFFRMM
                     RFGEFDCSEMSLSSYVASLGRSEPPFVAIPVFPSRYFRHSCIFVCAKSGIRKPEDLKG
                     KRIGVPEYQMTAPVWIRGILSDDYGVKVTEVEHFSGGEEEPGRDEKQKIDLPSSIKLS
                     PIGKTQTLSGVLAAGELDALVTARAPSTFKTTDAVKRLFPDYVEKEKAYYKRTKIFPI
                     MHTVVIRRDVYAKNPWVAQSLYKAFVASKKIAYEMYAQTAAMTSMLPWTSAQAEEART
                     EMGDDWWPYGLEPNRHVLETFLRYHHEQGLSKRLFKPEELFAKETLQSFKV"
     gene            3404..>3968
                     /locus_tag="VGO02_11300"
     CDS             3404..>3968
                     /locus_tag="VGO02_11300"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEV7478308.1"
                     /translation="MRLLVAILLLAAGAALGQDYPNKPIRVIVPYAAGGLPDTITRLI
                     QAKMADSLGQQLVVENRGGAGGISGTEAVAKAAPDGYTLLVADVGQVAINPHIFSKLP
                     YSPQKDLTPVTLIGTSALFLCLHPSVPANTFKELIAYIKANPGKVNYGSSGTGSIHHL
                     ATEALKAALKLDIVHVPYKGTGQAVPAL"
BASE COUNT          625 a         1291 c         1333 g          719 t
ORIGIN      
        1 cggcgatcga gggtcccgac gccgacgacc cttactcgcg cagcggcgcg ccggtgcagt
       61 tctcgccgca gcagttcaag ttcgccgtgc cctcgcagct cgagttccac ggcgactcgg
      121 gctacgccaa gctgttcgcg caggcgatcc tcaagctgcg ccagatcggc ggcacgccgg
      181 tcgagatcga cttcgcgccg ttcctcgagg cgggggcgct gctctacggg ccgtgggtcg
      241 ccgagcgcgc cgcgcacctc gagccgcaca tgaaggacat gctgccggtg gtccgcgaga
      301 tcctcgaagg cgcgaagaaa tactcggcgg tcgatttctt caaggcgcgc aaccgcctgg
      361 acgagctcaa gcagaagagc cgcgagacct tcaagcaggc cgacgtcatc ttcgtgccga
      421 ccacgccgac catctaccag ctcgcgcagg tcgagaagaa gccggtcgag ctgaacaacc
      481 acctgggcca ctacaccagc ttcaccaacc tgctcgacct cgccgcggtc agcctgcccg
      541 ccggcatgcg ccaggacggg gtgccgttcg gcgtcagcct gatcgcgccg gcgttcaccg
      601 acaaggcgct actcgcgctc ggcgcgcgct tccagggata aattcgggtc agcgccgccc
      661 ttcgttgtgg tcgtgccggt ggcgcttctc gtcgccgttg aagccgatgt ctgggtcgac
      721 ccacagcgcg agcagctggc cgccttccac cagctcggga tcggtgctcg gcttgtgcga
      781 ctgcacgtcg accaggaacc agccctcgcc caggatgtgg gcggcgtcga cgattcccga
      841 ggactcctcg tcgatggtga tgaactcggg ctgcttcgag tccgggtcga agaacttcgg
      901 cgtgtgcgcc gcgagctgga tcagctcgcg cgtgtcgatc gcgtacagcc agatcttggc
      961 gacgcggttg tcgccgcccg gatcctcgtc catcaggatg cggccgaggc ggtcgatggt
     1021 cacgttgtcg agcatcctgt ggccctcgtc gccgcgcagc atgatctgca ccgtgccgcc
     1081 ggcctcgggg cgctcgatgt cgtcgaagcg cagccgccac aggcggcagt tggtcgtgat
     1141 gctcgccgtg gtgacgaagt agaagtcgtt ctcgtggccc tcgcgggggt cccaggcgcc
     1201 gtcttcgcag cgctgcagcc gggtgacgtt gctcgcgttc gacaggcttt ccagcgcgag
     1261 gccggtcgtg atgcccgagg tgtcgcccag gctgaccagc tcgaagcggc cgctgccgat
     1321 gtagccgctg gtcgaattgc ccaggccgaa cgcgttgtct tcgccgttga cgtgcgcgcc
     1381 ttcgaccgag atgctgacgc cgtacagcag gccgttcatg aggcccgcct gctcgatcgg
     1441 ggtaccgccg ctctgcttgg tgccgatgta gaagtagacc tcgctgccgt tgttcggcag
     1501 ggggccggtc gcggcgctgc tgtcatccgt gagcgccacc agggtcttgt cctgcgagta
     1561 gggcgaggcg acggcgttct cataggccat ccgcccgaag cgtggcagct gccaggcctc
     1621 gcccatatgg gtcccggtgg cgacccgcgc ccaggcgcgg ccgtcggtca cctcttcgcc
     1681 gttcatgtag atgcgctcgg tcgtgccgcg gccgcgatgg aagaacgcgc ccggggcggg
     1741 caggtcggcc gagcaatgcc gctgccacag caccgacggc ggcacggtcg gcacgtacgc
     1801 gacgtgccgt cccgctgccg cgtcccagac gaacaccttg cccggcgaag gcgtgtggtc
     1861 ctggcccttc tttaccctga gcgtctccga gtcgatggtc cagcgcgaca cgaacgcgcc
     1921 gctggcgccg tgcgcccggg cgatccccgg cgggttggcg gtgagctcgt ggttcatgag
     1981 cagcgtgaac tcccggtggc cgctatcgaa ggcgcccagg ccgtcgggaa tgccgaccat
     2041 ccggtagccg ccgaccgagt ccccgaccgt caggatcgaa accagcttca ctccggcgat
     2101 ggtcggtcgc agatacggct cggtcgtcgt gctcgggccg accgcggtgc cggaagcctg
     2161 cgccggcttc ggcacgagcg cggcgctgcc ggcgatgagc agcggggttg cggccgctcc
     2221 cttgaggaac gagcgacggg aatttggttc gttcgatttc attgcggcac tcccttttgg
     2281 ttggtggctt gggccgcaag aaatatagaa gcgcagtgtt actgtttggt ggcacctcgg
     2341 aaggtgaatc gccgcgcgtt gcagcgcgga acagtcatat ttcttaaacc ttgaagctct
     2401 gcagcgtttc cttggcgaac agctcttcgg gcttgaacag gcgcttcgag aggccttgct
     2461 cgtggtggta gcgcaggaag gtctcgagca cgtggcgatt cggctcgagc ccgtagggcc
     2521 accagtcgtc gcccatctcg gttctggcct cttcggcctg cgccgaggtc cacggcagca
     2581 tcgacgtcat ggctgcggtc tgggcgtaca tctcgtaggc gattttcttc gatgcgacga
     2641 aggccttgta cagcgactgc gcgacccagg gattcttcgc gtacacgtcg cgccggatca
     2701 cgacggtgtg catgatcggg aagatcttcg tacgcttgta gtaggccttt tccttctcga
     2761 cgtagtcggg gaataggcgc ttgaccgcat cagtggtctt gaacgtggaa ggagcacggg
     2821 cagtcaccag cgcgtccagc tcgcctgcgg cgagcacgcc ggacagggtt tgcgtctttc
     2881 cgatcgggga aagcttgatc gaagaaggca ggtcgatctt ctgcttctcg tcgcggccgg
     2941 gctcttcttc gccgccggag aagtgctcga cctcggtgac tttcacgccg taatcgtcgg
     3001 acaggatgcc gcggatccac accggcgcgg tcatctggta ctcgggaacg ccgatgcgct
     3061 ttcccttcag gtcctcgggc ttcctgatgc cgctcttcgc gcagacgaag atgcaggagt
     3121 ggcggaagta gcgcgacggg aacacgggga tggcgacgaa cggcggctcg ctgcggccga
     3181 gcgaggcgac gtaggaggag agcgacatct ccgagcagtc gaactcgccg aaacgcatca
     3241 tgcggaagaa cgtttcctcg accggctggt tgaggtagat gaggtcgatg ccctccggct
     3301 gcacgcggcc gtcggccagc gcgcgggtgc gatcgtagtc ccagcaggcg aggctcaggc
     3361 gaagcatgcc gaagtatact tattggagga ggagattttt tccatgcgac ttctcgtagc
     3421 gattcttctg ctcgcggcgg gcgccgcgct cggccaggat tatccgaaca agccgatccg
     3481 cgtcatcgtc ccctatgcgg cgggcgggtt gcccgacacg atcacgcgcc tgatccaggc
     3541 gaaaatggcc gattcgctcg gccagcagct ggtggtcgag aaccgcggcg gcgcgggcgg
     3601 catctccggc accgaggcgg tggccaaggc cgcgccggat ggctatacgc tgctcgtcgc
     3661 cgacgtcggg caggtggcga tcaatccgca catcttctcc aagcttccct actcgccgca
     3721 gaaagacctg acgccggtga cgctcatcgg cacctcggcg ctgttccttt gcctgcatcc
     3781 atcggtgccc gccaatacgt tcaaggagct gattgcctat ataaaggcga atcccggcaa
     3841 ggtgaactac ggttcctccg gcaccggcag catccaccac ctcgcgaccg aggcgctgaa
     3901 ggcggcgctc aagctcgaca tcgtgcatgt cccctacaag ggcacggggc aggcggtccc
     3961 ggcgctgc
//
LOCUS       DASYJA010000523         1744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2348470, whole genome shotgun sequence.
ACCESSION   DASYJA010000523 DASYJA010000000
VERSION     DASYJA010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2348470"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(328..1629)
                     /locus_tag="VGO02_11305"
     CDS             complement(328..1629)
                     /locus_tag="VGO02_11305"
                     /inference="COORDINATES: protein motif:HMM:NF003707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HEV7478309.1"
                     /translation="MTHFIDRRLNPKGKSLGNRQRFLRRARTQIREAVQKSLKDSAVA
                     DLAKDRKIKISTRGTREPRFRLDPKSAGERDFVLPGNKEFSAGDEIKKPRSGDGAGRG
                     KDAADSGDNEDDFEFTMTQDEVLDIFFEDLELPNLVRTTLKETPSKTWRRAGITTAGS
                     PNQINLLRTMRHAQGRRLALRRPKLSEVKALEEALDLAEREDPPEPAKIAALKEQLAK
                     ANAKRKWVGFIDPVDVRFNAFAEQPVPTSQAVMFCLMDVSGSMGEREKDLAKRFYMLL
                     HLFLKRRYEKVDVVFIRHTHDAQEVDEQEFFYSRQSGGTIVSTALDKMLEIQKARYAT
                     ADWNIYAAQASDGYTQTGDARNCVELLNGEIMPLCQYYAYIEILDEREMEVFASDDSG
                     AELWRAYRTVAGLWDNFATKRISRPADIFPVFHELFRKAEA"
BASE COUNT          280 a          568 c          565 g          331 t
ORIGIN      
        1 tcgatctggt tgggatagac gtcgaggccg aggtccttga gcgcgatctc ctcgatggcg
       61 gcataggcgc ggtcgagggt gacgaagtcc cattcggagc cttcgaagag gagcttcgac
      121 attacccctc ctcaccatgg ccgggcttga cccggccacc cagcacgcca caggcgcaaa
      181 gagattcacg cggagacgcg gaggacgcgg agaagaaaaa gaactccgcg cactccgcgt
      241 ctccgcgtga gatatattca tttcgctcgc caacgctcgc tagctgggtg gccgggtcaa
      301 gcccggtcat ggtgatttgg agattcatca cgcttccgcc ttcctgaaca gctcgtggaa
      361 caccgggaag atatccgccg ggcgcgagat gcgcttggtg gcgaaattgt cccacagccc
      421 ggcgacggtg cggtaggcgc gccagagctc ggcgcccgag tcatccgacg cgaagacctc
      481 catctcgcgc tcgtccagga tctcgatata ggcgtagtac tggcacaatg gcatgatctc
      541 gccattgagc agctcgacgc agttccgcgc gtcgccggtc tgtgtgtagc cgtcgctcgc
      601 ctgcgccgcg tagatgttcc agtcggcggt ggcatagcgc gctttctgga tctccagcat
      661 cttgtcgagc gcggtcgaca cgatggtgcc gcccgactgg cgcgaataga agaactcctg
      721 ctcgtcgacc tcctgggcgt catgggtgtg gcggatgaag acgacatcga ccttctcgta
      781 gcgccgcttc aggaacaggt gcagcagcat gtagaagcgc ttggcgagat ccttctcgcg
      841 ttcgcccatc gagcccgaga cgtccatcag gcagaacatc accgcctggc tggtcggcac
      901 cggctgctcg gcgaaggcat tgaagcgcac atcgacggga tcgatgaagc cgacccattt
      961 gcgcttggcg ttcgccttgg cgagctgttc cttgagcgcg gcgatcttcg caggctccgg
     1021 cggatcctcg cgctcggcaa gatcgagcgc ctcttccagc gccttcacct cgctcagctt
     1081 gggccggcgc agcgccagcc tgcggccctg ggcgtggcgc atggtgcgca gcaggttgat
     1141 ctggttcggc gagccggccg tggtgatgcc ggcgcggcgc caggtcttcg acggcgtctc
     1201 cttcagcgtc gtgcgcacga gattgggcag ttccagatcc tcgaagaaga tgtcgagcac
     1261 ctcgtcctgc gtcatcgtga actcgaaatc gtcctcgttg tcgccgctgt cggccgcatc
     1321 cttgccgcgg ccggcgccgt cgccgctgcg cggcttcttg atttcatcgc cggcggagaa
     1381 ctccttgttg cccggcagca cgaaatcgcg ctcgcccgcg cttttcggat cgaggcggaa
     1441 gcgcggctcc ctcgtgcccc tggtcgagat cttgatcttg cggtccttgg cgaggtcggc
     1501 gaccgcggaa tccttgagcg acttctgcac cgcctcgcgg atctgcgtgc gcgcccggcg
     1561 caggaagcgc tggcggttgc cgagcgactt gcctttgggg ttcaggcggc ggtcgatgaa
     1621 gtgggtcatg ccacctcccc atcctccccc gtccttacgg gggaggcgcc gagcgtagcg
     1681 aggcggaggg ggaagcaaca cgcaacaaat cccccctcgc cgccttcggc agcgctcccc
     1741 ccgt
//
LOCUS       DASYJA010000524         3115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_714773, whole genome shotgun sequence.
ACCESSION   DASYJA010000524 DASYJA010000000
VERSION     DASYJA010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714773"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VGO02_11310"
     CDS             <1..973
                     /locus_tag="VGO02_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEV7478310.1"
                     /translation="RVKTRTLNGEEVELRISTLPTAFGEKLVMRIFDPEVLVRDFKEL
                     GFSDEDKTRWDEMTNRPNGIVLVTGPTGSGKTTTLYSTLKTLATPEVNVCTIEDPIEM
                     VEPAFNQMQVQNQIDLGFAEGVRALMRQDPDIIMVGEIRDLETAEMAVQSALTGHLVL
                     STLHTNDAPSAATRLMELGMPPYLLNATMNGIMAQRLVRTLCPHCKQPVEQHRAEDHA
                     AWDALVAPWKSKHPAKLYKPVGCLECRMTGFMGRVGIYETLLFTSAIKKLVNERADIA
                     AIREQAFKEGMKALRISGAMKVGAGLTTIEEVLKVAPPVQDAGGRPA"
     gene            970..>3115
                     /locus_tag="VGO02_11315"
     CDS             970..>3115
                     /locus_tag="VGO02_11315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV7478311.1"
                     /translation="MIDTRLFLDILLLAALSGGLVYLAMRFAARRTQRRLGQAQHNET
                     RFRDLTALSADWFWETDAEHRTTWISGGPTVATFFGDTSTYGRRIWEIPSVEVDARSL
                     EALRERLDAQQPFFDLEIARTDKRGARQIQIISGQSRLGADGAFLGYRGVGRDITEQR
                     RAEAALSQAKERLELALGGGNLAEWDYDLASRQVYLGGGWAAFLGREPAASVTRDTDL
                     IELVHREDRPAMSASMTGALKGNSATYSADYRVRTESGGWRWLRSVGQVTQRDGAGRA
                     LRMSGTVADIDDQRRAEEALRETEQRYRLLVELAPDGVVLHSNGIIEYANPAAARLFG
                     ATSPKRLAGLVLEEFVRPDQRERIRERARYLQAGPGWLGFEQRHVLTLDGREIVVDGA
                     AVSFLERGRLVVQSVFRDVTDQARAREALAEREQRFRDVAEASGEFVWECDREWRFTY
                     LSERVESVLGFARAELLGRRPHDFMPLGEERTVEAWLATHAARGAAWRGLEHRSVTKS
                     GAAIWLSVSAVPVRDAAGAPQGYRGTAADVTARKQAEARIEFLATRDVLTGLPNRLLL
                     GDRAGQAILAAARGRSQLALLCIDLDRFKLVNDSLGHPAGDALLRAVAERLGSTLRRE
                     DTLARLGGDEFVLLWNGLKSSEDAAALAQRVHSVLQRPFTVDGRTLNVGATIGIAIYP
                     GDGRDFAELLRNADAAMYDAKESGRGTFRFFSP"
BASE COUNT          459 a         1132 c         1103 g          421 t
ORIGIN      
        1 gcgcgtcaag acgcgcacgc tgaacggcga ggaggtggag ctgcgcattt cgaccctgcc
       61 caccgccttc ggcgagaagc tggtgatgcg catcttcgat ccggaggtgc tggtgcgcga
      121 cttcaaggag ctgggcttct ccgacgagga caagacgcgc tgggacgaga tgaccaaccg
      181 gcccaacggc atcgtgctgg tgaccgggcc gaccggctcg ggcaagacca ccaccctgta
      241 ttccacgctc aagacgctcg ccacgccgga agtcaacgtc tgcaccatcg aggacccgat
      301 cgagatggtg gagccggcgt tcaaccagat gcaggtgcag aaccagatcg acctcggctt
      361 cgccgaaggg gtgcgcgcgc tgatgcgcca ggaccccgac atcatcatgg tcggcgagat
      421 cagggacctg gagaccgccg agatggcggt gcagtcggcg ctcaccggcc acctggtgct
      481 ctcgacgctg cacaccaatg acgcgccgag cgcggcgacg cgcctgatgg agctgggcat
      541 gccgccgtat ctgctcaacg cgacgatgaa cggcatcatg gcgcagcgcc tggtgcgcac
      601 gctgtgcccg cactgcaagc agccggtcga gcagcaccgc gccgaggacc acgccgcctg
      661 ggacgcgctg gtcgcgccgt ggaagtcgaa gcacccggcg aagctctaca agccggtcgg
      721 ctgcctcgag tgccgcatga ccggcttcat gggccgggtg ggcatctacg agacgctgct
      781 ttttacctcc gccatcaaga agctggtgaa cgagagggcg gatattgccg ccatccgcga
      841 gcaggccttc aaggaaggca tgaaggcgct gcgcatcagc ggcgcgatga aggtcggcgc
      901 cggcctgacc accatcgagg aagtgctcaa ggtggcgccg ccggtgcagg acgcgggcgg
      961 gcggccggct tgatcgacac gcggctgttc ctcgacatcc tcctgctggc cgcgctttcc
     1021 ggcggcctgg tgtacctggc gatgcgcttc gccgcgcgcc gcacccagcg ccggctcggc
     1081 caggcgcagc acaacgagac gcgcttccgc gacctcaccg cgctgtcggc cgactggttc
     1141 tgggagaccg acgccgagca ccgcaccacc tggatctcgg gcggcccgac ggtggccacc
     1201 ttcttcggcg acacgtcgac ctatggcagg cgcatctggg aaatcccgag cgtcgaggtc
     1261 gacgcgcgct ccctcgaggc gctgcgcgag cgcctcgacg cccagcagcc gttcttcgac
     1321 ctggagatcg cgcgcaccga caagcgtggc gcgcgccaga tccagatcat ctccgggcag
     1381 tcgcgcctgg gtgccgacgg agctttcctc ggctaccgcg gcgtcggccg cgacatcacc
     1441 gagcagcgcc gcgccgaggc cgcgctgtcg caggcaaagg agcgcctcga gctcgccctg
     1501 ggcggcggca acctcgccga gtgggactac gacctcgcct cgcgccaggt ctacctcggc
     1561 ggcggctggg cggcgttcct cggccgcgag ccggcggcga gcgtgacccg cgacaccgac
     1621 ctgatcgagc tggtgcaccg cgaggaccgg ccggcgatga gcgcgtcgat gaccggggcg
     1681 ctgaagggga acagcgccac ctacagcgcc gactaccgcg tgcgcaccga aagcggcggc
     1741 tggcgctggc tgcgctcggt gggccaggtc acgcagcgcg acggcgccgg acgcgccctg
     1801 cgcatgtcgg gcacggtagc cgacatcgac gatcagcgcc gtgccgagga ggcgctgcgc
     1861 gagaccgagc aacgctatcg cttgctggtc gagctcgcgc ccgacggcgt cgtcctgcac
     1921 tccaacggca tcatcgagta cgccaacccg gcggcggcgc gcctgttcgg cgccacctcg
     1981 cccaagcggc tcgccggcct ggtgctcgag gagttcgtgc ggcccgacca gcgcgagcgc
     2041 atccgcgagc gggcgcgcta cctgcaggca ggccccggct ggctcggctt cgagcagcgt
     2101 cacgtgctca ccctggacgg ccgcgagatc gtggtcgacg gcgctgcggt ctccttcctc
     2161 gagcgcggcc gcctggtggt ccaatccgtt ttccgcgacg tcaccgacca ggcgcgcgcg
     2221 cgcgaggcgc tggccgagcg cgagcagcgc ttccgcgacg tcgccgaggc ctccggcgag
     2281 ttcgtctggg aatgcgaccg cgagtggcgc ttcacctacc tgtcggagcg cgtcgagtcg
     2341 gtgctcggct tcgcgcgcgc cgagctgctc ggccggcggc cgcacgactt catgcccctg
     2401 ggcgaggagc gcacggtcga ggcctggctc gcgacgcatg ccgcccgcgg cgccgcctgg
     2461 cgcggcctcg agcaccgctc ggtgaccaag tcgggcgcgg cgatctggct gtcggtgagc
     2521 gcggtgccgg tgcgcgacgc ggccggcgcc ccgcagggct accgcggcac cgccgccgac
     2581 gtcaccgcgc gcaagcaggc cgaggcgcgc atcgagttcc tcgccacgcg cgacgtcctg
     2641 accgggctgc ccaaccgcct gctgcttggc gaccgcgccg ggcaggcgat cctcgccgcg
     2701 gcgcgcggcc gctcgcagct cgcgctgctg tgcatcgacc tcgaccgctt caagctggtg
     2761 aacgactcgc tcggccaccc ggcgggcgac gcgctgctgc gcgcggtcgc cgagcgcctg
     2821 ggcagcacgc tgcgccgcga ggacaccctg gcgcgcctgg gcggcgacga gttcgtgctg
     2881 ctgtggaacg gcctcaagtc atcggaggac gccgcggcgc tggcgcagcg cgtgcattcc
     2941 gtcctgcagc ggccgttcac cgtagacggc cgcacgctca acgtcggcgc caccatcggc
     3001 atcgccatct acccgggcga cggccgcgat ttcgccgagc tcctgcgcaa cgccgacgcc
     3061 gcgatgtacg acgcgaagga gagcgggcgc ggcaccttcc gcttcttctc gcccg
//
LOCUS       DASYJA010000525         3051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_714843, whole genome shotgun sequence.
ACCESSION   DASYJA010000525 DASYJA010000000
VERSION     DASYJA010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714843"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..574)
                     /locus_tag="VGO02_11320"
     CDS             complement(251..574)
                     /locus_tag="VGO02_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478312.1"
                     /translation="MAPLKYEFTTVRQAALGVTLLGLVLVGIGGTIYKVISPEGWVTQ
                     AFGRSLSAGVAALGSLLMIVGLAWFSRGWSAPRSGNRFPELLIFTFAGAGLLYLAQLW
                     TKGTF"
     gene            789..1781
                     /locus_tag="VGO02_11325"
     CDS             789..1781
                     /locus_tag="VGO02_11325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003272206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV7478313.1"
                     /translation="MRLSVLDQSPVISGLGARRALEETIALARRADELGYHRYWLAEH
                     HAIAALADPCPEILLARLGAETRRIRVGSGGVLLPYYSAFRTAESFRMLEALYPGRID
                     LGIGRAPGGDGRTAQAVNGGKFPDADRFPEQVWELVGHLEGSLPADHPFKRVRLQPEL
                     DSVPQVWLLGSSDFSGALAAQLGLPFAFAHFINARGGDEVTDFYQKNFRPSARCPKPE
                     TLVCTFLICGESDEDAERLAASIDLRRLHMALNIDSAVPTPEEAARHRYSPEERQYVM
                     SQRARAVIGGPQTCRRALEEMAARYGADEMMVLTITGDYATRLRSYERLIEAMN"
     gene            complement(1751..2521)
                     /locus_tag="VGO02_11330"
     CDS             complement(1751..2521)
                     /locus_tag="VGO02_11330"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV7478314.1"
                     /translation="MIAELGPWALGFVVLVCLAAGFAHGAIGFGFPVVATPLVALVLD
                     IRSAIALLAPVTLVMVLLSVMRGGSLMELARRFWYLPLVIGLGSWLGTRLLLAAPPEP
                     FLLVLAGVVLLYLNLDRLGLAGSSIVKAKPISFGLGFGFVAGVFEAIANVAGPVLLVY
                     FMLLGLAPTQMVQALNLCFTAGKATQVATWAAAGAMTPALWMAVAAFTVPSVAALFAG
                     MRVRSRIDAATYRRCLRAALWVMALLLIGQFIASISRS"
     gene            complement(2518..>3051)
                     /locus_tag="VGO02_11335"
     CDS             complement(2518..>3051)
                     /locus_tag="VGO02_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEV7478315.1"
                     /translation="YYDYGMLDRAKSALNRVLDVLEPGVPVVALEPGCLSVFRDEMKQ
                     LFPRDERAARLKVFSLSEFLLEKNFKVKNGASVVVHSHCHQKAIWGAESDIDLLRAAG
                     CSITAPDTGCCGMSGSFGYKPQHYEASKRIFGLALQPALAAAPAAEVVACGFSCREQI
                     EDLGGRPTLHLAELLAG"
BASE COUNT          576 a         1039 c         1008 g          428 t
ORIGIN      
        1 cgagcaggcg cgcgagcagc tggtgagggg gcctcgcccg ttcccgagaa tgaaattgaa
       61 tcgggagaga aaagatctgc tcgactttca atacgaggat ttcaccctcg aggcctacga
      121 tccccatccg gcgatcaaag cgcccatagc ggtttgactg cggccgtcca ggtctggata
      181 aaaaaagggc ccgactgtag ggggaaaaaa gtcgggcccg ggggaggagt cgttgtcggt
      241 gaggcgagcg ctagaacgtt cccttcgtcc agagctgcgc caggtacagc agcccggcgc
      301 cagcgaaggt gaagatgagc agttcgggaa agcggttgcc cgagcggggt gcggaccagc
      361 cgcgcgagaa ccacgccagc ccgacaatca tgaggagcga gccgagcgcc gcgacgccgg
      421 ccgacaggct gcggccgaag gcctgcgtca cccagccctc cggcgatatg accttgtaga
      481 tggtgccgcc gatgcccacc agcacgagcc cgagcagcgt gacgcccagg gcggcctgac
      541 ggacagtcgt gaattcgtat ttgagcggag ccacgcacaa gtactgcaac gtgcgtgcca
      601 gaacccgatt aactagtcct aacagacgct tgaggtcgat ccgtgaaaca gttccaaggg
      661 tgcggcaggt accgattgtc gggccacggc gacgacgtat gccacattcc cccgaacgca
      721 ttgaatgcac aggcggttta tgtgtcgctg gcggccgaca aaggcggctg ccttaacatc
      781 tgggcccgat gcgcctctct gtactcgatc aatcgccggt gatcagcggg ctcggcgcac
      841 gccgcgccct cgaggaaacg atcgcgctcg cgcgccgcgc cgacgagctg ggctaccacc
      901 gctactggct ggccgagcac catgcgatcg ccgcgctcgc cgatccctgt cccgagatcc
      961 tgcttgcccg cctgggcgcg gagacgcggc gcatccgtgt cggcagcggc ggcgtgctgc
     1021 tgccgtacta cagcgcattc cgcacggcgg agagcttccg catgctggag gcgctctacc
     1081 ccggccgcat cgacctcggc atcgggcgcg ccccgggcgg cgacggccgc accgcgcagg
     1141 cggtgaacgg cggcaaattt ccggacgcag atcgcttccc cgagcaggtg tgggagctgg
     1201 tcgggcatct cgagggcagc ctgccggcgg atcacccgtt caagcgcgtg cgcctgcagc
     1261 ccgagctcga ctcggtgccg caggtctggc tgctcggctc ctccgacttc agcggcgcgc
     1321 tcgccgcgca gctcggcctg cccttcgcct ttgcgcactt catcaacgcg cgcggcggcg
     1381 acgaagtcac ggatttttat cagaagaatt ttcgtccctc cgccagatgt ccgaagcctg
     1441 aaaccctggt gtgcaccttc ctcatctgcg gcgagagcga cgaggacgcc gagcgtctcg
     1501 ccgcgtcgat cgacctgcgg cggctgcata tggcgctgaa catcgactcg gcggtgccca
     1561 caccggagga ggcggcgcgg caccggtaca gccccgaaga gcgccaatac gtcatgagcc
     1621 agcgcgcccg cgcggtgatc ggcggcccgc agacgtgccg gcgcgccctg gaggagatgg
     1681 cggcgcgcta cggcgcggat gaaatgatgg tgctcaccat caccggcgac tacgccacgc
     1741 gcctgcgctc ctacgagcgg ctgatcgagg cgatgaactg accgatgagc agcagcgcca
     1801 tcacccagag cgccgcgcgc aggcagcgcc ggtaggtcgc cgcgtcgatg cggcttcgca
     1861 ctcgcatgcc ggcgaagagc gccgccaccg aaggcacggt gaacgccgcc accgccatcc
     1921 acagcgccgg ggtcatcgcc ccggccgcgg cccacgtcgc cacctgggta gccttgccgg
     1981 ccgtgaagca caggttgagc gcctgcacca tctgcgtcgg cgcaagcccg agcagcatga
     2041 agtagaccag cagcaccggg ccggcgacgt tggcgatcgc ctcgaatacg ccggcgacga
     2101 agccgaagcc gaggccgaaa gagatgggtt ttgcttttac tattgaactt cccgcaagac
     2161 cgagacgatc aaggttcagg taaagcagga cgactccagc cagcaccaga aggaacggct
     2221 cgggcggcgc cgcgagcagc agccgcgtgc cgagccagga gccgaggccg atcaccagcg
     2281 gcaggtacca gaagcggcgc gcgagctcca tcaggctgcc gccgcgcatc accgagagca
     2341 gcaccatcac caaagtcacc ggggcgagca gcgcgatcgc cgacctgatg tcgagcacca
     2401 gcgccaccag cggcgtggcg acgaccggga agccgaagcc gatcgcgccg tgcgcgaagc
     2461 cggccgccag gcagaccagc accacgaagc ccagcgccca cgggcccagc tcggcgatca
     2521 tccggcgagc agctcggcga ggtgcagcgt cggccggccg ccgagatcct cgatctgctc
     2581 gcggcacgag aagccgcagg ccaccacttc ggccgccggg gcggcggcga gcgcgggctg
     2641 cagcgccagg ccgaaaatcc gcttcgatgc ctcgtagtgc tgcggcttgt agccgaacga
     2701 gcccgacatg ccgcagcagc cggtgtcggg cgccgttatg ctgcaacctg cggctcgaag
     2761 cagatcgata tcgctctctg ctccccaaat cgccttttga tggcaatgcg agtggaccac
     2821 cacgctggcg ccgtttttca ccttgaagtt tttctccagg agaaattcgg aaagcgagaa
     2881 aaccttgagc cgagccgcgc gctcgtcccg cggaaagagc tgcttcatct cgtcgcgaaa
     2941 caccgaaagg cagcccggct ccagcgccac caccggcacg cccggctcga gcacgtcgag
     3001 cacgcgattc agcgcgctct tggcgcgatc gagcatgccg tagtcgtagt a
//
LOCUS       DASYJA010000526         5923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2348857, whole genome shotgun sequence.
ACCESSION   DASYJA010000526 DASYJA010000000
VERSION     DASYJA010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2348857"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VGO02_11340"
     CDS             <1..445
                     /locus_tag="VGO02_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7478316.1"
                     /translation="QNAFRMIEEGVKPETLDRAMEDFGMPMGPAELADTVGLDICLAA
                     GKALAKGDARVPQFLADKVAAGQLGKKTGQGIYRWADGKPVKSEAGPVDQRLIDSLIE
                     PYLHEAEAAVKEGIVEDAELADAGLIFGTGFAPFRGGPLHYLKSK"
     gene            489..830
                     /locus_tag="VGO02_11345"
     CDS             489..830
                     /locus_tag="VGO02_11345"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEV7478317.1"
                     /translation="MPMPADANQNGDIFGGWIMSQVDIAGGTVAGRVARGRVATVAVK
                     EFVFKQPVQIGDVVSFYVELERIGTTSITVTVEVYAERRPEEPKTVKVTEATLTYVAI
                     DRDGRPRPVPR"
     gene            832..1569
                     /locus_tag="VGO02_11350"
     CDS             832..1569
                     /locus_tag="VGO02_11350"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEV7478318.1"
                     /translation="MLLDPATLAAVALLGLGAAVIGSVGGFGTGIILTAALAPLIGVK
                     ALVPVLAVAGVIINAGRFWFYRRYLERRTFAIVLAAALPFLVLGAWIYSILDARTLGV
                     VLGLVIIASVPLRRILKKHEIVIGPAGLAAGGGVFGLASGVATGTGVILISLLLGSGL
                     AGPAVLATDALITIALDFSKTLLFQRFDLLDGQHFFTGVVIGVASIPGSAAGAWLVRR
                     LHAHLHVLFMEALILAGGGFMLWHSWT"
     gene            1584..2405
                     /locus_tag="VGO02_11355"
     CDS             1584..2405
                     /locus_tag="VGO02_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7478319.1"
                     /translation="MKILQGKTAAITGAGSGLGRAMALAFAQEGMKLALADVDQKGLD
                     ETVSLLGNASLLSMKVDVSKEVDVQAFCDRIEKPYLVCNNAGVSPLGAVWENSVGDWQ
                     WILGVNLWGVIHGVRAFAPRLIAQNDGHIVNTASVAGLISPPGSGAYNVTKHAVVALS
                     ESLHHDLQERKSAVGVSVLCPAYVPTGISRSERNRPAGLSSGEKSELTKAKEANLKKA
                     VASGRLTADDVAKAVVAAVKEDRFYILTHPRIKGAIQARMEDILNERAPRDPLRL"
     gene            complement(2402..3040)
                     /locus_tag="VGO02_11360"
     CDS             complement(2402..3040)
                     /locus_tag="VGO02_11360"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter"
                     /protein_id="HEV7478320.1"
                     /translation="MSSLQEYLRFLVTLTAVLDPFLAVPIFLTVTASYGVPARVALLR
                     VVAVTVFVVLAAAALLGDTILTLVGASLSAFRVGGGLVLLLMALAMLNAQAGGVRQSE
                     AEAAELESGELRGVVPLAVPLLAGPGAISTTIIAAEKGGGAVAHLAAILGCIAVVSLL
                     TWLVLRAADAIRNRLGTTGLNIATRLLGLLLAALAIQTMAEGLRQLFPGLAS"
     gene            complement(3037..3684)
                     /locus_tag="VGO02_11365"
     CDS             complement(3037..3684)
                     /locus_tag="VGO02_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEV7478321.1"
                     /translation="MLRLCGFHVSNYHNKVRIALLEKGIEHVDDSGCKPSQEDAFLAR
                     SPMGKVPFLELGEGALRESQVICEYLEDAFPQKPLYPKDALERAKVRELITFLELHVE
                     LVARRLYPLLFGRTISDETRQSVEKALPKGIRAFRALARFDPYLAGKELTFADCAAFV
                     HLPVVSIATKMAYGRDFLDELPQVKPYIKMLGERPAFKRVNEDRKAAQAALAPKK"
     gene            complement(3702..4181)
                     /locus_tag="VGO02_11370"
     CDS             complement(3702..4181)
                     /locus_tag="VGO02_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEV7478322.1"
                     /translation="MRRLLLFPLLAVAHAAAADTISGKVVAVANGDTLTMVDGAAKRH
                     RIRLAEIDAPERKQAFGAESRKSLAEICLQKAAVAETVVKDRRLIGKVSCAGVDAGTE
                     QVRRGMAWVFAGTVLPNSPLPEMEANARLRALGLWAGDQPEPPWEWRKRQGAPAAKK"
     gene            complement(4199..5437)
                     /locus_tag="VGO02_11375"
     CDS             complement(4199..5437)
                     /locus_tag="VGO02_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV7478323.1"
                     /translation="MNFDYTPKVQELRKQLIAFMNEHVYPNEHEWHEHVKSDKRWQPV
                     PLIEKLKVKAREAGLWNLWRPKSHGGTLTNLEYAPLCEIMGREHWAPEVFNCSAPDTG
                     NMETLLKYGTPEQVKQWCEPLLEGKIRSAFLMTEPAVASSDATNIQTRIAREKNDYVI
                     NGTKWWSSGAGDPRCKIYIVMGKTDPDNTSRHSQQSMVLVPSDAKGIKVLRHLPVFGY
                     DDAPHGHMELDLKDVRVPASNVLLGEGRGFEIAQGRLGPGRIHHCMRLIGQAERALEK
                     MCRRATARIAFGKAVADQGVTRERIAEARIMIDSARFMVLNAAWKMDTVGNKGAKKEI
                     GMIKVLAPNMACQVIDWAMQVHGGGGVSEDFGLARAYAHARTLRFADGPDEVHRNQIA
                     KLELGEQTTVAEALAKVRRI"
     gene            complement(5434..>5923)
                     /locus_tag="VGO02_11380"
     CDS             complement(5434..>5923)
                     /locus_tag="VGO02_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008341699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV7478324.1"
                     /translation="IGWLPKNIPPEGATTVVHGDFRLDNTIFHPTEARILAVLDWELS
                     TLGDSLADFSYHCMSWHIPPGQFRGIAGLNLAELGIPGESEYVARYCARTGRKAIDPS
                     HWDYYMAYNLFRMAAILQGIAKRVLDGTAASQHATEAASKTVPLAELGWQQAEKILRR
                     AA"
BASE COUNT          896 a         2036 c         1983 g         1008 t
ORIGIN      
        1 gcagaacgcc ttccgcatga tcgaggaggg cgtgaagccc gagacgctcg accgcgccat
       61 ggaagacttc ggcatgccga tgggcccggc ggagctcgcc gacaccgtcg gcctggacat
      121 ctgcctcgcc gccggcaagg ccctcgccaa gggcgacgcc agggtgccgc agtttctcgc
      181 cgacaaggtg gcggcgggcc agctcggcaa gaagaccggc cagggcatct accgctgggc
      241 cgatggaaaa ccggtcaaga gcgaagcggg cccggtcgac cagcgcctga tcgactcgtt
      301 gatcgagccg tatctgcacg aggccgaggc ggcagtgaag gaaggcatcg tcgaggacgc
      361 cgagctggcc gacgccggcc tgatcttcgg caccggcttc gcgccgttcc gcggcggccc
      421 gctgcactac ctgaagagca agtagcttgg acagtcttcc cgcgggtaag cagccggcgc
      481 tgcgcgtcat gccgatgccg gcggacgcca accagaacgg cgacatcttc ggcggctgga
      541 tcatgtcgca ggtcgacatc gccggcggca ccgtcgccgg gcgcgtcgcg cgcggccgcg
      601 tcgccaccgt cgcggtgaag gaattcgtct tcaagcagcc ggtgcagatc ggcgacgtgg
      661 tgtcgttcta cgtcgagctc gagcgcatcg gcaccacctc gatcaccgtg acagtcgagg
      721 tgtacgccga gcgccggccg gaggagccga agaccgtcaa ggtcaccgag gcgaccctca
      781 cctacgtcgc catcgaccgc gacggccggc cgcgcccggt gccgcgctag cttgctgctc
      841 gaccctgcca cgctcgccgc ggtagcgctg ctcggcctgg gcgcggcggt gatcggcagc
      901 gtcggcggct tcggcaccgg catcatcctg accgcggcgc tcgcgccgct catcggcgtc
      961 aaggcgctgg tgccggtcct ggcggtggcc ggcgtcatca tcaacgccgg gcgcttctgg
     1021 ttctaccggc gctacctcga gcggcgcacc ttcgccatcg tgctcgcagc cgcgctgccc
     1081 tttctcgtgc tcggcgcctg gatctacagc atcctcgatg cgcgcaccct cggcgtggtg
     1141 ctcggcctgg tgatcatcgc ctcggtgccg ctgcgccgca tcctcaagaa gcacgagatc
     1201 gtgatcgggc cggccggcct cgccgccggc ggcggcgtgt tcggcctggc gagcggcgtc
     1261 gccaccggca ccggcgtgat cctgatctcg ctgctgctcg gctcgggcct ggccggcccg
     1321 gcggtgctcg ccaccgacgc gctgatcacc atcgcgctcg acttctccaa gacgctgctg
     1381 ttccagcgct tcgacctgct cgacggccag cacttcttca ccggcgtggt gatcggcgtc
     1441 gcctcgattc ccggcagcgc cgccggcgcc tggctggtcc ggcgcctgca cgcgcacctg
     1501 cacgtgctgt tcatggaggc gctgatcctc gccggcggcg gcttcatgct gtggcattcg
     1561 tggacctgag cggaggaggc gcaatgaaga tcctgcaagg aaagacggcg gcgatcaccg
     1621 gcgccggcag cggcctgggc cgcgcgatgg ctttggcatt cgcgcaggag ggcatgaagc
     1681 tcgcccttgc ggacgtggat cagaaagggc tcgacgaaac ggtttccctc ctcggtaacg
     1741 cctctctcct ctccatgaaa gtcgatgttt ccaaggaggt cgacgtacag gccttctgcg
     1801 accggatcga gaaaccgtat ctcgtctgca acaacgccgg cgtttcgccg ctcggcgccg
     1861 tctgggaaaa cagcgtgggc gactggcagt ggatcctcgg cgtcaacctg tggggcgtga
     1921 tccacggcgt gcgcgccttc gcgccgcggc tcatcgcgca gaacgatggc cacatcgtca
     1981 acaccgcctc ggtcgccggc ctcatctcgc cgccgggctc cggcgcctac aacgtcacca
     2041 agcacgccgt ggtcgcgctg tcggaatcgc tgcaccacga cctgcaggag cggaaatcgg
     2101 cggtcggggt gtcggtgctt tgcccggcgt acgttcctac cgggatttcc cgttcggaac
     2161 gcaatcgccc tgcgggcctt tcatcaggcg agaagtcgga actgacgaaa gcgaaagaag
     2221 cgaacctgaa gaaagcggtg gcatccggac ggctgacggc ggacgacgtc gcgaaggcgg
     2281 tcgtggccgc agtgaaggag gaccgcttct acatcctcac gcacccgcgc atcaagggcg
     2341 cgatccaggc gcgcatggag gacatcctca acgagcgcgc gccgcgcgac ccgctgcggc
     2401 tctaggaagc gagcccgggg aagagctgcc tcaacccctc ggccatcgtc tggatggcga
     2461 gggcggccag cagcagaccc agaagccgcg tggcgatgtt gagcccggtg gtgcccagcc
     2521 ggttgcggat cgcgtcggcc gcccgcagca cgagccaggt gagcaggctg acgacggcga
     2581 tgcagccgag gatcgcggcg aggtgcgcga cggcgccgcc gcccttctcc gccgcgatga
     2641 tggtggtgct gatcgcgccg gggccggcga gcagcggcac ggcgaggggc accacgcccc
     2701 tgagctcgcc gctctccagc tcggccgcct ccgcctccga ctgcctcacg ccgcccgcct
     2761 gggcattcag cattgcgagc gccatgagca ggagcaccag gccgccgccg acgcggaagg
     2821 ccgacaggct cgcgccgacc agcgtgagga tcgtgtcgcc gagcagcgcc gcggcggcga
     2881 gcacgacgaa caccgtgacg gccaccacgc gcagcagcgc gacgcgcgcc gggacgccgt
     2941 acgacgcggt gacggtgagg aagatcggca cggcgaggaa cggatcgagc accgcggtca
     3001 gggtgaccag gaaccgcagg tactcctgca gagagctcac ttcttcggcg cgagcgcggc
     3061 ctgcgccgcc ttgcggtctt cgttcacccg cttgaacgcc gggcgctcgc cgagcatctt
     3121 gatgtagggc ttgacctgcg gcagctcgtc gaggaagtcg cggccgtagg ccatcttcgt
     3181 cgcgatgctg accaccggca ggtgcacgaa ggcggcgcag tcggcgaagg tcagctcctt
     3241 gccggcgagg tagggatcga acctggcgag ggcgcgaaag gcgcggatgc cctttggcag
     3301 cgccttctcg actgactgcc tggtctcgtc gctgattgtt ctgccgaaca gcaaggggta
     3361 gagccgcctg gcgaccagct cgacgtgcag ctccaggaac gtgatcagct cgcggacctt
     3421 ggcgcgctcc agcgcatcct tcggatacag cggcttctgc ggaaaggcat cctcgaggta
     3481 ctcgcagatc acctgcgatt cgcgcagggc gccttcgccg agctccagga acggcacctt
     3541 gcccatcggg ctgcgcgcga ggaacgcgtc ttcctgcgac ggcttgcagc ccgagtcgtc
     3601 gacgtgctcg atgcctttct cgagcagggc gatgcgcacc ttgttgtgat agttgctgac
     3661 gtgaaagcca cagagcctga gcatcgcgcc tccgattgcc gctatttctt cgccgccggc
     3721 gcgccctggc gcttgcgcca ctcccaggga ggctcgggct ggtcgcctgc ccacaagccg
     3781 agcgcgcgca gcctggcgtt cgcttccatc tcggggagcg gtgaattggg aagcacggtc
     3841 cccgcaaaca cccaggccat cccgcggcgc acctgttcgg tgccggcgtc gacgccggcg
     3901 cagctcacct tgccgatcag gcgccggtcc ttcaccaccg tttcggctac cgccgccttc
     3961 tgcaggcaga tctcggcgag ggacttgcgc gactcggcgc cgaaggcctg cttgcgctcg
     4021 ggcgcatcga tctccgcgag gcggatcctg tgccgcttcg cggcgccgtc caccatggtg
     4081 agcgtatcgc cgttggcgac cgcgacgacc ttgcccgaga tcgtgtccgc ggctgccgcg
     4141 tgggcaaccg ccagcagggg aaacagcagc agccgtcgca tgcgcagcac tcccggtgtc
     4201 agatgcgcct gactttggcg agcgcctcgg cgaccgtggt ctgctcgccg agctccagct
     4261 tggcgatctg gttgcggtgc acctcgtcgg ggccgtcggc gaagcgcagc gtgcgcgcgt
     4321 gcgcgtaggc gcgcgccagg ccgaagtcct cgctcacgcc gccgccgccg tgcacctgca
     4381 tcgcccagtc gatcacctgg caggccatgt tgggcgccag caccttgatc atgccgatct
     4441 ccttcttcgc tcccttgttg ccgacggtgt ccatcttcca cgccgcgttg agcaccatga
     4501 agcgcgcgct gtcgatcatg atgcgcgcct cggcgatgcg ctcgcgcgtc acgccctgat
     4561 ccgctactgc ttttccaaaa gcaatccttg ccgtggcccg acggcacatt ttctccagcg
     4621 cgcgctccgc ctgcccgatc aagcgcatgc agtggtggat gcggcccggc ccgaggcggc
     4681 cctgcgcgat ctcgaagccg cggccctcgc cgagcagcac gttggaggcc ggcacgcgca
     4741 cgtccttcag gtcgagctcc atgtggccgt gcggcgcgtc gtcgtagccg aacaccggca
     4801 aatggcgcag cactttaatt ccctttgcat ctgaaggtac aagaaccatg gattgctggc
     4861 tgtgcctgga ggtgttgtcc gggtcggtct tgcccatcac gatgtagatc ttgcagcgcg
     4921 ggtcgccggc gccgctcgac caccacttgg tgccgttgat cacatagtca tttttttcgc
     4981 gggcgatcct ggtttgaatg ttggttgcat ccgacgaggc gaccgccggc tcggtcatca
     5041 ggaacgccga gcggatcttg ccttcgagca gcggctcgca ccactgcttc acctgctccg
     5101 gcgtgccgta cttgagcagc gtctccatgt tgccggtgtc gggcgccgag cagttgaaca
     5161 cctccggcgc ccagtgctcg cggcccatga tctcgcacag cggcgcgtac tcgaggttgg
     5221 tgagcgtgcc gccgtgcgac ttcggccgcc acaggttcca caagccggcc tctctggcct
     5281 ttacttttaa tttttctatc aaaggaacgg gctgccagcg cttgtcgctc tttacgtgct
     5341 cgtgccactc atgctcgttg gggtagacgt gctcgttcat gaaggcgatg agctgcttgc
     5401 gcagctcctg caccttcggg gtgtaatcga agttcatgct gctctcctga ggatcttttc
     5461 ggcctgctgc cagccgagct cggccagcgg cacggtcttg ctcgcggctt ccgtcgcgtg
     5521 ctggctcgcc gcggtaccgt cgagcacgcg cttcgcgatg ccttgcagga tcgccgccat
     5581 gcggaacagg ttgtacgcca tgtaatagtc ccagtgtgag ggatcgatgg cctttcttcc
     5641 ggtcctggcg caataccgcg cgacatattc gctctcgccg gggatcccga gctccgcgag
     5701 gttcaggccc gcgatccccc ggaactgtcc gggcgggatg tgccagctca tgcagtgata
     5761 cgagaaatcg gcgagcgagt cgccgagcgt cgagagctcc cagtcgagca ccgcgaggat
     5821 gcgcgcctcg gtcggatgga agatcgtgtt gtcgaggcgg aagtcgccgt gcaccaccgt
     5881 cgtcgcgccc tcgggcggga tgttcttcgg cagccagccg atc
//
LOCUS       DASYJA010000527         6155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1440078, whole genome shotgun sequence.
ACCESSION   DASYJA010000527 DASYJA010000000
VERSION     DASYJA010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6155
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440078"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1539
                     /locus_tag="VGO02_11385"
     CDS             <1..1539
                     /locus_tag="VGO02_11385"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEV7478325.1"
                     /translation="ATVPEVVEKLVKLGFRVAVESGAGDAANFGDDTYKAAGAQIAPD
                     AAALWSSADIVFKVRAPTAEEVGRMREGQTLISFIWPAQNPELMQQLAGRQVTVLAMD
                     SLPRMLSRAQKMDALTSMAGITGYRAVIEAAHNFGRFLTGQITAAGKIPPAKVFIAGA
                     GVAGLAAIGTAVGLGAIVRANDTRAEVADQVASMGGEFVKVEYEEEGGGGGGYAKVMS
                     EGFQKAQREMYAKQAREVDIIITTALIPGKPAPRLITAEMVRSMKPGSVIVDLAAEQG
                     GNCELTEPGKVVVKHGVTIVGYTDLPSRLAKQSSTLYATNLFRLAEELCKTKDGVINV
                     NMDDDAIRGLTVIKDGSITWPPPPLKLPAPPPKQVSQQKTSTPAAKGHSHGAGEPMPA
                     RSLAIVFGVGALVLLFIGNNAPPAFLGHLTVFVLACFVGYMVVWNVTPALHTPLMSVT
                     NAISSIIAIGALVQVAPPLEDFGPDRPAGWILGLALVAIALTSINMFGGFAVTRRMLQ
                     MFRK"
     gene            1550..2962
                     /gene="pntB"
                     /locus_tag="VGO02_11390"
     CDS             1550..2962
                     /gene="pntB"
                     /locus_tag="VGO02_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - NAD(P)+ transhydrogenase
                     (AB-specific) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEV7478326.1"
                     /translation="MSGTLATVAYLGATVLFILSLGGLSSPESSRRGNLYGMVGMAIA
                     VLATVLGPQVEPKGIAWIVGAMALGGSIGLYAARVVKMTQMPELVALMHSLVGLAAML
                     VGYANYIDPAATAHFKGVERTIHEAEIYAGVLIGAVTFSGSLIAFGKLSAKISGKPLL
                     LPGRHWLNLAGLLLVIWLGTRFVGAPAIESGMLPLVLMTLIALLFGVHMVMAIGGADM
                     PVVVSMLNSYSGWAAAATGFMLSNDLLIVTGALVGSSGAILSYIMCRAMNRNFISVIA
                     GGFGTGGGTAAPSGEAPAGEVVAVSAPETAALLREAKNVVVVPGYGMAVAQAQHTVYE
                     ITKLLREKGVNVRFGIHPVAGRMPGHMNVLLAEAKVPYDIVMEMDEINPDFPASDVAM
                     VIGANDIVNPAAQEDPASPIAGMPVLEVWKAKTSVVMKRSMASGYAGIDNPLFYKDNN
                     RMLFGDAKKMLDEVLAALKA"
     gene            3000..3830
                     /locus_tag="VGO02_11395"
     CDS             3000..3830
                     /locus_tag="VGO02_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEV7478327.1"
                     /translation="MISLKRGARSLRALLSRKLSPDVLKIGVSPRIFHPQPDARGIQS
                     KTLQYLEQSVAHWVMSRDVLVFMVPTVDKDGLLHRSNIRLADYARVLDALVLQGGADV
                     SPGSYGEQPLRQDWAGDRVRDVYEIELVHEFIEAGKPVLGICRGAQLINVALGGSLYQ
                     DVNTQLPESQRHVTDEYEKNLHEIRFAPGSGLARLYPRVERPRVTSIHHQSIKALGRG
                     LKVEAWSEPDGVIEAVRGGGPGYVFAVQWHPEFHHPGNQGALDCAPILEEFLSAARGA
                     "
     gene            3827..5035
                     /locus_tag="VGO02_11400"
     CDS             3827..5035
                     /locus_tag="VGO02_11400"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV7478328.1"
                     /translation="MSEYLFLPQLPLAFSQLAMFGALLLAGLLAGEAARRYFSMPRIT
                     GYVLAGVLLGPQATGLLDSNALYDLRLVVDLAIGLVVFELGFRLSFDWLSRNRWLFAT
                     AIAESVLCFCAIYGALVYFDFRPLLAATAAAIGAATSPPVVMLVAQELRAEGQITERM
                     LLFTAVNTVFAFVMLTLLLPFRHIERAPDWQPAVLYPIYVLAGSALAGYLACSVLLAL
                     ARWLGKREGRQLVLLVAMVVLTIGIAHSLKLSVPLALVTFGMLSRNLDRRHALLPVRF
                     GELGQLFFVVLFVITGASLEFQALGVAAAGAVAAFVVLRFLGKALAILLFGHLSGIRP
                     GGAGLLAIALLPMSGLAVVMVRDTVSLYPSFGRELAAVVLSAVVVLELLGPLATQFAL
                     RRAGEAHPDV"
     gene            5028..6155
                     /locus_tag="VGO02_11405"
     CDS             5028..6155
                     /locus_tag="VGO02_11405"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HEV7478329.1"
                     /translation="MSETGFTASPGMSLGVELELQLVRPHDLDLARDAGDLLARLEKR
                     KLPGAVKPEITESMIELNSSVHERCSGLALELDLMRRAVVEEAGVLNVRISGGGAHPF
                     HDWTDRRIYPNERFRYIVERYGYLAKQFTVFGQHIHIGCPSGDEAVYLVHMMTRYVPH
                     FIALSAASPYYQGEDTSFQSSRLTAVSAFPLAGHMPFVRDWAEFLEYFGKMRQFGIVA
                     SMKDFYWDIRPKPEYGTIEIRVCDTPLTVERAAQLAAYAQALAAYHLAERPREASRAV
                     YLVNSHNRFEACRYGFRGELVEPYAGVKKKIGDDILETLDLVAPHARRLDCGEPLAAL
                     AAAVRADQADANWLRERHAARGALADVVRDACGRWEKRAAT"
BASE COUNT          906 a         2114 c         2109 g         1026 t
ORIGIN      
        1 gccaccgtgc cggaggtggt ggagaaactg gtcaagctgg gattccgggt cgcggtagag
       61 tccggcgctg gcgacgcagc caacttcggc gacgacactt acaaggccgc gggcgcgcag
      121 atcgcgccgg acgcggcggc gctctggtcc tcggccgaca tcgtgttcaa ggtgcgcgcg
      181 ccgaccgccg aggaagtcgg ccggatgcgc gaagggcaga ccctgatcag cttcatctgg
      241 cccgcgcaga accctgaact gatgcagcag ctcgcggggc gccaggtcac cgtactggcc
      301 atggactcgc tgccgcgcat gctgagccgc gcgcagaaga tggacgcgct gacttccatg
      361 gccggcatca ccgggtatcg cgccgtgatc gaggcggcgc ataacttcgg ccgcttcctc
      421 acgggccaga tcaccgccgc cggcaagatt cctccggcca aggttttcat cgccggcgcc
      481 ggcgtcgccg gcctggcggc gatcggcacg gcggtcggcc tgggtgccat cgtgcgcgcc
      541 aacgatacgc gtgccgaagt ggccgaccag gtggcgtcga tgggcggcga gttcgtgaag
      601 gtcgagtacg aagaggaagg cggcggtggc ggcggctacg ccaaggtcat gagcgagggc
      661 ttccagaagg cccagcgcga gatgtatgcg aagcaggccc gtgaggtgga catcatcatc
      721 accaccgcgc tgatccccgg caagcccgcg ccgcggctga tcacggcgga gatggtccgc
      781 tcgatgaagc ccggcagcgt gatcgtcgac ctggcggccg agcagggcgg caactgcgag
      841 ctgaccgaac ccggcaaggt cgtggtcaag catggcgtga cgatcgtcgg ctataccgat
      901 ctgcccagcc ggctggccaa gcagtcctcc acgctctatg cgaccaatct gttccgcctg
      961 gccgaagagc tctgcaagac caaggacggc gtcatcaacg tcaatatgga cgacgacgcc
     1021 atccgcggcc tgacggtcat caaggacggc agcatcacct ggccgccgcc gcccctcaag
     1081 ctgccggctc cgccgccgaa acaggtttcc caacagaaaa cttctacccc ggccgcaaag
     1141 ggacactcgc acggcgcggg cgagccgatg ccggcgcgct cgctcgccat cgtgttcggg
     1201 gtcggcgcgc tggtgctgct gttcatcggc aacaacgcgc cgccggcttt cctcgggcac
     1261 ctcaccgtgt tcgtgctcgc ctgcttcgtc ggctacatgg tggtgtggaa cgtgacgccg
     1321 gcgctgcaca cgccgctcat gagcgtgacc aacgcgatca gcagcatcat tgccatcggt
     1381 gccctggtgc aggtggcgcc gccgctggag gacttcggcc cggatcgtcc cgcgggctgg
     1441 atcctcggcc tcgcgctcgt cgccatcgcg ctcaccagca tcaacatgtt cggcggcttc
     1501 gccgtgacgc ggcgcatgct gcagatgttc cgcaaatgag gcgctcgccg tgagcgggac
     1561 gcttgcgacc gtcgcctacc tcggcgccac ggtcctgttc atcctcagcc tgggcggcct
     1621 gtccagtccg gagagctcgc gccgcggcaa tctctacggc atggtcggca tggcgatcgc
     1681 cgtgctcgcc accgtgctcg gcccgcaggt cgagccgaag gggatcgcgt ggatcgtggg
     1741 cgcgatggcg ctcggcgggt cgatcggcct gtatgcggcg cgcgtggtga agatgacgca
     1801 gatgccggag ctggttgccc tgatgcacag cctggtgggc cttgcggcca tgctggtcgg
     1861 ctacgccaac tacatcgacc cggcggccac cgcgcacttc aagggcgtgg agaggacgat
     1921 ccacgaggcg gagatctacg ccggcgtgct gatcggcgcc gtgacgttct cgggctcgct
     1981 catcgccttc ggcaagctct cggccaagat cagcggcaag ccgctcctgc tgcccggcag
     2041 gcactggctc aatctcgccg gcctgcttct ggtgatctgg ctcggcacgc gtttcgtcgg
     2101 tgcccctgcc atcgaaagcg ggatgctgcc gctcgtgctg atgaccctga tcgcgctgct
     2161 cttcggcgtc cacatggtga tggcgatcgg cggcgccgac atgccggtcg tggtgtcgat
     2221 gctgaacagc tattcgggct gggcggcggc ggccaccggc ttcatgctgt cgaacgacct
     2281 gctgatcgtg accggcgccc tggtcggttc ctccggtgcg atcctttcct acatcatgtg
     2341 ccgcgcgatg aaccgcaact tcatcagcgt gatcgccggc gggtttggaa ccgggggcgg
     2401 aacagccgcc ccctcaggcg aggcaccggc gggtgaggtg gtcgcggtca gcgcgcccga
     2461 aaccgcggcc ctgctgcgcg aggcgaagaa cgtggtcgtg gtgccgggct acggcatggc
     2521 ggtcgcccag gcgcagcaca cggtctacga gatcaccaag ctgctgcgcg agaagggcgt
     2581 caacgtgaga ttcggcatcc atccggtcgc cgggcgcatg ccgggccaca tgaacgtgct
     2641 cctggcggag gcgaaggtgc cctacgacat cgtgatggag atggacgaga tcaacccgga
     2701 ttttcccgcc agcgatgtcg cgatggtgat cggcgcgaac gacatcgtga acccggccgc
     2761 gcaggaagac ccggcgagcc cgatcgccgg catgccggtg ctcgaggtgt ggaaggcgaa
     2821 gacctcggtg gtcatgaagc gcagcatggc ctcgggctac gcgggcatcg acaatccgct
     2881 cttctacaag gacaacaacc gcatgctgtt cggcgacgcg aagaagatgc tcgacgaggt
     2941 gctcgccgcg ctgaaggcct gaggtggtag aggccttcgt caggccttag actcgcgcgg
     3001 tgatctcgct caagcgtggc gcgcgctcgt tgcgcgccct tctctcgcgc aagctttcgc
     3061 cggacgtgct gaagatcggc gtctcgccgc gcatcttcca cccgcagccc gacgcgcgcg
     3121 gcatccagtc gaagacgctg caatacctcg agcagtcggt ggcgcactgg gtcatgtcgc
     3181 gcgatgtgct ggtcttcatg gtgccgacgg tggacaagga cggcctgctc caccgcagca
     3241 acatccgcct cgcggattac gcgcgcgtgc tcgacgcgct ggtgctgcaa ggcggcgccg
     3301 acgtcagccc ggggagctac ggcgagcagc cgctgcgcca ggactgggcc ggcgaccgcg
     3361 tacgcgacgt ctacgagatc gagctggtgc acgagttcat cgaggccggc aagccggtgc
     3421 tcggcatctg ccgcggcgcg cagctcatca acgtcgcgct cggcggctcg ctctaccagg
     3481 acgtgaacac ccagctgccc gagtcgcagc gccacgtcac cgatgaatac gagaaaaacc
     3541 tgcacgagat ccgcttcgcc ccgggctcgg gcctcgcgcg gctgtatccc cgcgtcgagc
     3601 ggccgcgcgt cacctcgatc caccaccagt cgatcaaggc gctcggccgc ggtctgaagg
     3661 tggaggcctg gtcggagccc gacggggtga tcgaggcggt gcgcggcggc gggccgggct
     3721 acgttttcgc ggtgcaatgg cacccggagt tccaccaccc gggcaaccag ggcgcgctcg
     3781 actgcgcgcc gattctcgag gaattcctct cggccgcgcg gggagcatga gcgagtacct
     3841 cttcctgccg cagctgccgc tcgccttctc gcagctggcg atgttcggcg cgctgctcct
     3901 ggcgggactg ctcgccggcg aggccgcgcg acgctacttc tccatgccgc gcatcaccgg
     3961 ctatgtgctg gcgggggtgc tccttggacc gcaggccacc gggctcctcg acagcaacgc
     4021 gctctacgat ctgaggctgg tcgtcgacct tgcgatcggc ctggtggtgt tcgagctcgg
     4081 ctttcgcctg agcttcgact ggctgagcag gaaccgctgg ctgtttgcca ccgcgatcgc
     4141 cgaaagcgtg ctgtgtttct gcgcgatcta cggcgcgctc gtctacttcg acttccgccc
     4201 gctgctcgcc gcgaccgccg cggccatcgg cgccgccact tcgccgcccg tggtgatgct
     4261 ggtggctcag gaactgcgcg ccgaggggca gatcaccgag cggatgctgc tcttcacggc
     4321 ggtgaacacg gtattcgcct tcgtcatgct gacgcttctg ctgccgttcc ggcacatcga
     4381 gcgcgcaccc gattggcaac ccgccgtgct gtacccgatc tacgttctcg ccggttccgc
     4441 gctcgccgga tatctcgcct gcagcgtcct gctggcgctc gcgcggtggc tgggcaagcg
     4501 cgaagggcgc cagctggtgc tgctcgtcgc gatggtggtg ctgaccatcg gcatcgccca
     4561 cagcctcaag ctctcggtgc cgctcgcgct ggtgaccttc ggcatgctgt cgcgcaacct
     4621 cgaccgcagg catgcgctgc tgccggtgcg cttcggcgag ctcggccagc tgttcttcgt
     4681 cgtgctgttc gtgatcaccg gcgcgagcct cgaattccag gcgctgggcg tcgccgcggc
     4741 cggcgccgtc gccgcgttcg tcgtgctgcg ctttctcggc aaggcgctcg ccatcctgct
     4801 gttcggccat ctgtcgggga tccggccggg gggcgccggg ctcctcgcca tcgccctgct
     4861 gccgatgtcg ggcctcgcgg tggtgatggt gcgcgacacc gtgtcgctgt atccctcgtt
     4921 cggccgcgag ctcgccgcgg tggtgctgtc ggcagtcgtg gtgctcgagc tgctcggccc
     4981 gctcgccacg cagttcgccc tgcgccgggc cggcgaggcg catcccgatg tctgagaccg
     5041 gcttcaccgc ctcgcccggc atgagcctcg gcgtcgagct cgagctgcag ctcgtgcggc
     5101 cgcacgacct tgacctggcg cgcgacgccg gcgacctgct cgcgcgcctg gagaaacgca
     5161 agctgcccgg cgcggtcaag cccgagatca ccgagagcat gatcgagctg aacagctcgg
     5221 tgcacgagcg ctgctcgggc ctcgcgctgg agctcgacct gatgcgccgc gcggtggtag
     5281 aggaggccgg ggtgctcaac gtgcggatct cgggcggcgg cgcgcacccg ttccacgact
     5341 ggaccgaccg gcgcatctat ccgaacgagc gcttccgcta catcgtcgag cgctacggct
     5401 acctcgccaa gcagttcacc gtcttcgggc agcacatcca catcggctgc ccgagcggtg
     5461 acgaggcggt gtacctggtc cacatgatga cgcgctacgt gccgcatttc atcgcgctgt
     5521 cggctgcttc accctactac cagggtgagg acacctcgtt ccagtcctcg cggctcaccg
     5581 cggtcagcgc cttcccgctc gccgggcaca tgccgttcgt gcgcgactgg gccgagttcc
     5641 tcgagtactt cggcaagatg cggcagttcg gcatcgtcgc cagcatgaag gacttctact
     5701 gggacatccg gcccaagccc gagtacggca cgatcgagat ccgggtgtgc gacaccccgc
     5761 tcacggtgga gcgcgccgca cagctcgccg cctatgcgca ggccctcgcc gcgtaccatc
     5821 tggcggagcg gccgcgcgag gcgtcgcgcg ccgtgtacct ggtcaacagc cacaaccgct
     5881 tcgaggcctg ccgctacggc tttcgcggcg agctggtgga gccctatgcc ggtgtgaaaa
     5941 agaagatcgg cgacgacatc ctcgagacgc tcgacctggt ggcgccgcac gcgcgccggc
     6001 tggactgcgg cgagccgctc gccgcgctcg ctgccgcggt gcgcgccgac caggcggacg
     6061 cgaactggct gcgcgagcgc cacgccgcgc gcggcgcgct cgccgacgtg gtgcgcgatg
     6121 cctgcgggcg ctgggagaag cgcgcggcta cttga
//
LOCUS       DASYJA010000528         4548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2349577, whole genome shotgun sequence.
ACCESSION   DASYJA010000528 DASYJA010000000
VERSION     DASYJA010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2349577"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..2836)
                     /locus_tag="VGO02_11410"
     CDS             complement(146..2836)
                     /locus_tag="VGO02_11410"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEV7478330.1"
                     /translation="MRRLTTGILLLLFAALAYSQPLQKTYSIEGITEHRLQNGLRVLT
                     LPDPGIDTITVHIAYMVGSRHEGYGEKGMAHLLEHLLFKGTKRHPNIKDELTSRGARW
                     NGTTSTDRTNYFETFAATDANLDWAISLEADRMVNSRVSREDLDSEMTVVRNEFESGE
                     NSPGAVLFQRMQQLAFPWHNYGNAIIGSRSDIEQVPIEKLQAFYRTWYQPDNALLIVA
                     GRFDEKRALELVAKHFGAVPRPSRTLPALYTREPTQDGERNVTLRRAGDNQLLMSLYR
                     APAGSHPDYPAVDVLVQVLGDAPSGRLHRMLVQKGLASSAWGSERALYDPGFVSFGAT
                     LDKSAPLAPARAALIEAVEGVVKDGVQSAEFERARTRLLNDFEKSQLDTAGLVRALSE
                     FQAMGDWRLYFLYRERLRKVTLEDVKRVAASYLKPANRVLGEFIPTDAPERAEIPEVR
                     GLQETLAAFKPADESVRLGETFDPSPANIESRVVRQQLANGIQAAFLPKKTRGGRVVA
                     SLSLHWGDEKSLTGREAACSFAGSMLARGTAKKSRAELQDAFDKLNASVSIGGDGATI
                     EVRRDNLAAALRLAAEALREPAFPATEFEEMKRAALTSAEAQRGDPGALAGVRLARHL
                     HAYPEGHPHYTPTVDERIGWMRKATLKDAVACYRELFGATGADFAVVGEFDADEAARL
                     AEELFGGWKTPHAFTRVPARYFERARFENDVLTPDKANAVLRGGLNLKMRDDHPDFPA
                     MVLANHLLGGSSVARVPARVREKEGLSYSTFTSFTSSAFDESASFRVSSIFAPENRAR
                     VESAIREELVRAAREGFSPAEVETGKKALLEARHLSRTQDRALAGRLGSYLFAKRTFA
                     WDIEFEKKIAALTPEQVNAALRRHIDPERLSVVTAGAFKK"
     gene            complement(2833..3147)
                     /locus_tag="VGO02_11415"
     CDS             complement(2833..3147)
                     /locus_tag="VGO02_11415"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEV7478331.1"
                     /translation="MNLWYTIAGMAIVTYLARSSFLLLPPGVETPPWLRRALRYVPAA
                     VLTAIWAPELALQKEVISLTLHNERLLAGLVAIAVAWRFRATFVTIVSGLLALHLFDW
                     LL"
     gene            complement(3144..3830)
                     /locus_tag="VGO02_11420"
     CDS             complement(3144..3830)
                     /locus_tag="VGO02_11420"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEV7478332.1"
                     /translation="MSLYRPAFAEGVRASLPVLLGVVPFGVITGVAMVASGIPPLAAV
                     LMSLLVFAGASMVASAQLLASHTPALLVVLTTLIINLRFMMYSASLRLHFAHAPLRWR
                     LLLGYLTADNVYGLMLQRFSDHPDDPDKMPFFLGAGSAIWLVWQLAVLAGVVIGAGVP
                     ASWRLEFAAPLAFIAMTIPLLRDRSMVVAALAAGITVIAAHALPLKLSVLLAAATGIG
                     AGMLFERKNR"
     gene            complement(3877..4032)
                     /locus_tag="VGO02_11425"
     CDS             complement(3877..4032)
                     /locus_tag="VGO02_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478333.1"
                     /translation="MRTEFCAKSPDPKQCEARAKEKQARGDKIREACKDKKGDEFKAC
                     AREQRKK"
     gene            complement(4336..>4548)
                     /locus_tag="VGO02_11430"
     CDS             complement(4336..>4548)
                     /locus_tag="VGO02_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEV7478334.1"
                     /translation="ALGKIRFLADGSAQWAKALGLELDLNARGMGTRMNRFSMLVDNG
                     VVKQLNVEGPGKFEVSDAGTMLKQLG"
BASE COUNT          697 a         1490 c         1614 g          747 t
ORIGIN      
        1 cgatttcgtg atcatcgcct gggcgatctt catggccatc aaggtgatca accgcatacg
       61 cgagcccgaa gcggccgcgg tgccgcgcca ggagcagctg ctcgaagaaa tcagggacct
      121 gctgaagaaa gaatagggac agaccctatt ttttgaacgc tcctgcagtc acgaccgaca
      181 ggcgctcggg gtcgatgtgc cggcgcagcg cggcgttcac ctgctcgggc gtcagcgcgg
      241 cgattttctt ctcgaactcg atgtcccagg cgaaagtgcg cttggcgaac aggtaggagc
      301 cgagccggcc ggcgagcgcg cggtcctgcg tgcgcgacag gtggcgcgcc tcgagcagcg
      361 ctttcttgcc ggtctcgacc tcggcggggc tgaagccttc gcgcgcggcg cgcacgagct
      421 cctcgcggat cgcgctctcg acgcgggcac ggttctcggg cgcgaagatc gaggagacgc
      481 ggaacgacgc cgactcgtcg aaggcgctcg aggtgaagct ggtgaaggtg ctgtaggaga
      541 ggccttcctt ctcccgcacg cgcgccggca cgcgcgccac cgaggagccg cccagcaggt
      601 ggttggcgag caccatcgcc gggaagtcgg gatggtcgtc gcgcatcttc aggttgagcc
      661 cgccgcgcag cacggcgttg gccttgtcgg gcgtaagaac gtcgttctcg aagcgcgcgc
      721 gctcgaaata gcgcgccggc acccgcgtga atgcgtgcgg cgtcttccag ccgccgaaca
      781 gctcctcggc caggcgcgcc gcctcgtcgg cgtcgaactc gcccaccacc gcgaagtccg
      841 cgccggtcgc cccgaacagc tcgcggtagc aggctaccgc gtccttcagc gtggccttgc
      901 gcatccagcc gatgcgctcg tcgacggtcg gggtgtagtg cgggtggcct tccgggtagg
      961 cgtgcaggtg gcgcgccagg cgcacgccgg cgagcgcgcc cgggtcgccg cgctgcgcct
     1021 ccgcgctggt cagcgccgcg cgcttcatct cctcgaactc ggtggcgggg aaagcgggct
     1081 cgcgcagcgc ttcggccgcc aggcgcagcg ccgcggccag gttgtcgcga cgcacttcta
     1141 tggtcgcgcc gtcgccgccg atcgagacgc tggcgttgag cttgtcgaag gcgtcctgca
     1201 gctcggcgcg gctcttcttc gccgtgccgc gcgccagcat gctgccggcg aagctgcacg
     1261 ccgcctcgcg gccggtcagg ctcttctcgt cgccccagtg cagggagagg ctcgccacga
     1321 cgcggccgcc gcgcgtcttc ttcggcagga aggcggcctg gatgccgttg gccagctgct
     1381 gcctgacgac gcgcgactcg atgttggccg gcgagggatc gaaggtctcg cccaagcgca
     1441 ccgactcgtc ggcgggcttg aacgccgcca gcgtctcctg caggcccctc acctcgggaa
     1501 tctcggcgcg ctcgggcgcg tcggtcggga tgaactcgcc gagcacgcgg ttggccggct
     1561 tcaggtagct cgcggcgaca cgcttcacgt cctcgagcgt caccttgcgc aggcgctcgc
     1621 gatacaggaa gtagagccgc cagtcgccca tcgcctggaa ctcggaaagc gcgcgcacca
     1681 ggcccgcggt gtcgagttgc gacttctcga agtcgttgag caggcgcgtg cgcgcgcgct
     1741 cgaactcggc gctttgcacg ccgtccttca ccacgccctc gaccgcctcg atgagcgcgg
     1801 cgcgcgccgg cgcgagcggc gccgacttgt cgagggtggc gccgaacgag acgaagccgg
     1861 ggtcgtacag ggcgcgctcc gagccccagg cgctgctggc cagccctttc tgcaccagca
     1921 tgcggtgcag ccgcccgctg ggggcgtcgc cgagcacctg gacgaggacg tcgaccgccg
     1981 gatagtcggg gtggctgccg gccggcgcac ggtacagcga catcagcagc tggttgtcgc
     2041 cggcgcggcg cagcgtgacg ttgcgctcgc cgtcctgcgt cggctcgcgc gtgtagagcg
     2101 cgggcagggt gcgcgagggc cgcggaacgg cgccgaagtg cttcgcgacc agctcgaggg
     2161 cgcgcttctc gtcgaagcgg ccggcgacga tgagcagcgc gttgtcgggc tggtaccagg
     2221 tgcggtagaa cgcctgcagc ttctcgatcg gcacctgctc gatgtccgag cgcgagccga
     2281 tgatggcgtt gccgtagttg tgccagggga aggcgagctg ctgcatgcgc tggaacagca
     2341 ccgcgccggg gctgttctcg cccgactcga actcgttgcg caccaccgtc atctcgctgt
     2401 cgaggtcctc gcgcgagacg cgcgagttca ccatccggtc ggcctccagg ctgatcgccc
     2461 agtcgaggtt ggcgtcggtg gcggcgaagg tctcgaagta gttggtgcgg tcggtggagg
     2521 tggtgccgtt ccagcgcgcg ccgcggctgg tcagctcgtc cttgatgttc ggatggcgct
     2581 tggtgccctt gaacagcagg tgctctagca ggtgcgccat gcccttttcg ccgtagccct
     2641 cgtggcgcga gcccaccatg taggcgatgt gcacggtgat ggtgtcgatg cccgggtcgg
     2701 gcagggtgag cacgcgcagg ccgttctgca ggcggtgctc ggtaatcccc tcgatggagt
     2761 atgttttctg gaggggctgg gaatacgcca gcgccgcgaa cagcagcagc agtatcccgg
     2821 tagtcagtcg cctcatagca gccagtcgaa aaggtggagg gcgaggagcc cggatacgat
     2881 cgtaacgaag gtagcgcgga accgccatgc caccgcgatc gcgacgaggc cggccagcag
     2941 cctctcgttg tgcagcgtca gggatatgac ttccttttgc agcgccagtt ccggcgccca
     3001 gatcgccgtc agcaccgccg ccggaacgta gcgcagggcg cgccgcagcc acggcggcgt
     3061 ctcgacgccc ggcggcagca gcaggaacga ggagcgggcg aggtaggtaa cgatggccat
     3121 gcccgcgatg gtgtaccaga gattcatctg tttttacgtt caaaaagcat cccggctcct
     3181 atgccggtcg cggcggccag cagcaccgac agcttgaggg gcagggcgtg cgcggcgatc
     3241 acggtgatgc ccgcggcgag cgcggcgacg accatggagc ggtcgcgcaa tagcggaatg
     3301 gtcatggcga tgaaggcgag cggtgcggcg aactcgaggc gccacgaggc cggcacgccg
     3361 gcgccgatca cgacgccggc cagcaccgcc agctgccaga ccagccagat cgcggaacct
     3421 gcgccgagga agaacggcat cttgtccgga tcgtccggat ggtcggagaa gcgctgcagc
     3481 atcaggccgt agacgttgtc ggcggtgagg tagcccagca gcagccgcca gcgcagcggg
     3541 gcgtgcgcga agtgcaggcg cagcgaggcg ctgtacatca tgaagcgcag gttgatgatc
     3601 agcgtggtga gcaccaccag cagcgcgggg gtgtgcgagg cgaggagctg cgccgaggcg
     3661 accatcgagg cgccggcgaa gaccaggagc gacatgagca ccgccgcgag cggcgggatg
     3721 ccgctcgcca ccatcgccac gccggtgatg acgccgaacg gcacgacgcc gaggagcacg
     3781 ggcagcgaag cgcgtactcc ctcggcgaaa gcgggacgat aaagagacat cggggccccg
     3841 attctacgag ggccccgatg cttttatcgg ttgaagctac ttcttgcgct gctcgcgcgc
     3901 gcaggccttg aactcgtcgc ccttcttgtc cttgcaggcc tcgcgaatct tgtcaccgcg
     3961 ggcctgcttt tccttggccc tggcttcgca ctgcttggga tccggcgact tggcgcagaa
     4021 ctcggtgcgc acgcaggctt cgtgctccgc gcccttcttg gattcacagg ccttgcgtgc
     4081 agccttcgcg ttcgcctggc gctcttctca gcgcttcggg tccttcgcct tcgagcagtc
     4141 gacctgcatt ttctgatgcc ccttaccggc agcgggcggg gtcgactgag caaaagtgac
     4201 ggtggacgcg gcgagaagac aggcggcgat gatgactggg agcttcatgg ggtggtcctt
     4261 tcttgggtca agactcttcc attaacgtcc cggctcgcac gctgccgacc gtcagttgta
     4321 actctttgta tctatctatc cgagctgctt gagcatggtg ccggcgtcgg acacctcgaa
     4381 tttgcccggg ccctcgacgt tgagctgctt caccacgccg ttgtcgacca gcatcgagaa
     4441 gcggttcatg cgcgtgccca tgccgcgggc gttgaggtcg agctccaggc cgagcgcctt
     4501 cgcccattgc gcgctgccat cggcgaggaa gcggatcttg ccgagcgc
//
LOCUS       DASYJA010000529         5537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_899173, whole genome shotgun sequence.
ACCESSION   DASYJA010000529 DASYJA010000000
VERSION     DASYJA010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899173"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2084)
                     /locus_tag="VGO02_11435"
     CDS             complement(<1..2084)
                     /locus_tag="VGO02_11435"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEV7478335.1"
                     /translation="MIDKSFSPAAVEQRWYQRWEAAGYFKAGAAQAGKEQRPAYTIQL
                     PPPNVTGTLHMGHAFQQTLMDALIRYHRMRGFNANWVAGTDHAGIATQIVVERQLEAE
                     GKTRHDLGREKFVERVWQWKQQSGSTITRQMRRLGASANWEYADTEGQKAGYFTMDAK
                     MSRAVVEVFVRLYEEGLIYRGKRLVNWDPVLGTAVSDLEVDTEETMGTMWRIVYPFVD
                     GPQPSTDKEGRPLKLRGMTIATTRPETMLADGAVAVHPEDPRYRHLIGKRVILPLCDR
                     TIPVVADEMVEREFGTGSVKITGAHDFNDYGAALRHNLPLIVIFTLDAKINQNGPKQF
                     QGMDRYVARKAVVAQLEREGFLVGADPHRMMLPKSGRTGVVVEPMLTDQWFVKMEGMA
                     RDGLEVVKRGEVKFFPEHWTATYNHWLENIQDWCISRQLWWGHQIPAWYDAEGSIHVA
                     RTEKEALEKSEGKPLKRDPDVLDTWFSSQLVPFSSLGWPDKTPDLEKFLPSDVLVTGN
                     DIIFFWVARMIMATRHFTGKVPFRHVYINAIVRDAEGEKMSKSKGNTLDPLDLIDGSS
                     LEKLLEKSTTGLLKGEHRERIEKYVRTHYPQGISAYGADAVRFTFASLASFAITLNFD
                     LGRCEGYRNFCNKLWNATRFVLMNTEGKDCGADESQPVKLSFIDRWIISQLQRVETEV
                     VQGFEEYRFDNLA"
     gene            complement(2081..2461)
                     /locus_tag="VGO02_11440"
     CDS             complement(2081..2461)
                     /locus_tag="VGO02_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478336.1"
                     /translation="MSDEENRENNPLGERVDALLKRAKPERAPGDVPVLTEVVADELA
                     RRGTGIDRAVLERIALEVERAVLARLGPELERVVNENLTRALDTALGKALDAVRAELA
                     VSVRQMVREAVATSMVNALKSDAQ"
     gene            complement(2454..2882)
                     /locus_tag="VGO02_11445"
     CDS             complement(2454..2882)
                     /locus_tag="VGO02_11445"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEV7478337.1"
                     /translation="MTTIDFYFNAEDRLQVACRLASKAAAQGKRMLIYAPQADTASRI
                     DKLLWTWQAIGFLPHCAAGDELAAQTPVLIATGEETPADCGLLLNLSGECPPHFARFE
                     RLFEVVGADEAERAAGRSRYRFYLDRGYQIANHDLAGSHE"
     gene            complement(2879..4357)
                     /locus_tag="VGO02_11450"
     CDS             complement(2879..4357)
                     /locus_tag="VGO02_11450"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEV7478338.1"
                     /translation="MEFSIKTLSPETAKAGCVVLGTYANQELTPAARRADQAAKGALR
                     KALADLSGKAGSSLLLRGVAGIAAERVLLVSLGERGEFGESAFRDAVRGAANALKDLG
                     AKDAAFFLVDMKVGERHLTWNLRHAVLGLREAFYRFDQLKSQKKAPAAALQHVIFPFT
                     AKKELQQTLAEAAATADGIDLAKTLGNLPPNICTPAYLAEEAKKLAKQYKLGVEVLER
                     ADMQKLGMGALLAVASASHHPPKLIVLRYNGAAKGRKPIALVGKGITFDTGGISLKPA
                     GEMDEMKFDMSGAGSVLGAIRALAGMKAPVNVVGVIPTCENMPGGHATRPGDIVTTLS
                     GQTVEILNTDAEGRLILCDALTYAARFNPEKVVDIATLTGACVVALGHVASGLFANDE
                     ALAAELVAAADDASDRVWRLPLWEEYQEGLRSNFADFANIGGRPGGAITAACYLARFT
                     RKQRWAHLDVAGTAWKSGREKGSTGRPVPLLVRFALRHAARK"
     gene            4428..5504
                     /gene="lptF"
                     /locus_tag="VGO02_11455"
     CDS             4428..5504
                     /gene="lptF"
                     /locus_tag="VGO02_11455"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEV7478339.1"
                     /translation="MIFQRALLREFAGLAGAVFMTLFAIALTTRLIRLLGQAAGGKIP
                     SDAVIAFLGFFALGTLPVLLSLTMFISVLMTLTRSWRDSEMVIWFGSGLSLTAWLKPV
                     LIFALPQIAVITALSIFISPWAAQMGAQYATRLETRDDVSRVSPGVFGETANRERVFF
                     VESVSGDAAAVQNVFISSSHQSKSGVSMSRSGHTEAAENGDRFIVLEQGRRYEGAPGD
                     EQYRITEFERYAARIETKEGKEPAVTHRTLPTWALIINPTNQNLGELLWRFGIPVSAL
                     VLVLMAIPMSFVNPRAGRSINLMIALLTYIVYSNLLSVSVARVAQGRMDFALGSWLVH
                     AAMVLLLLFMFAQRMQIIRLRLAR"
BASE COUNT          792 a         1840 c         1903 g         1002 t
ORIGIN      
        1 gccaggttgt cgaagcggta ctcctcgaag ccctgcacca cctcggtctc gacgcgctgc
       61 agctggctga tgatccagcg gtcgatgaag gagagcttca ccggctgcga ctcatccgcg
      121 ccgcagtcct tgccctcggt gttcatcagc acgaagcgtg tcgcgttcca cagcttgttg
      181 cagaagttgc ggtagccctc gcagcggccg aggtcgaagt tgagcgtgat ggcgaagctc
      241 gccagcgagg caaaggtgaa gcgcaccgcg tcggcgccgt aggcggaaat gccctgcggg
      301 tagtgggtgc ggacgtattt ctcgatgcgc tcgcggtgct cgcccttgag gagcccggtg
      361 gtcgattttt ccagcaattt ttctaatgaa cttccatcga tcaggtcgag cgggtcgagc
      421 gtgttgccct tggacttcga catcttctcg ccctcggcgt cgcgcacgat ggcgttgatg
      481 tagacgtggc ggaagggcac tttgcccgtg aagtggcgcg tcgccatgat catgcgcgcc
      541 acccagaaga agatgatgtc gttgccggtg accagcacgt ccgacggcag gaatttctcc
      601 aggtccggcg tcttgtcggg ccagccgagg gagctgaacg gcacgagctg cgaggagaac
      661 caggtgtcga ggacgtccgg atctcgcttc aggggtttgc cttccgattt ctccagggcc
      721 tctttctcgg tacgggcaac atggatgctg ccttcggcgt cgtaccacgc cgggatctgg
      781 tggccccacc agagctggcg cgagatgcac cagtcctgga tgttctccag ccagtggttg
      841 taggtcgcgg tccagtgctc gggaaagaac ttcacctcgc cgcgcttcac cacctcgagg
      901 ccgtccctcg ccatgccttc catcttcacg aaccactggt cggtgagcat cggctcgacc
      961 accacgccgg tgcggcccga cttgggcagc atcatccggt gcgggtcggc gccgaccagg
     1021 aagccctcgc gctctagctg ggcgaccacc gccttgcgcg cgacgtagcg gtccatgccc
     1081 tggaactgct tcggcccgtt ctggttgatc ttcgcgtcga gcgtgaagat gacgatcagc
     1141 ggcaggttgt ggcgcagcgc ggcgccgtag tcgttgaagt cgtgcgcgcc ggtgatcttc
     1201 accgagccgg tgccgaactc gcgctcgacc atctcgtcgg ccaccaccgg gatggtgcgg
     1261 tcgcacagcg gcaggatgac gcgcttgccg atcaggtggc ggtagcgcgg atcctccggg
     1321 tgcaccgcca ccgcgccgtc ggccagcatg gtctcgggcc gggtggtggc gatggtcatg
     1381 ccgcgcagct tgagcggcct gccttccttg tcggtcgagg gctgcgggcc gtcgacgaag
     1441 gggtagacga tgcgccacat cgtgcccatc gtctcctcgg tgtcgacctc gaggtccgag
     1501 acggccgtgc cgagcaccgg gtcccagttg acaagtcgtt tccctctgta aatcaatccc
     1561 tcttcataaa gccggacgaa gacttccacg acagcacgcg acatcttcgc gtccatcgtg
     1621 aaatagccgg ctttctgccc ttcggtgtcc gcgtattccc agttcgccga tgcgcccagg
     1681 cgccgcatct ggcgcgtgat ggtggagccg gactgctgct tccactgcca cacgcgctcg
     1741 acgaatttct cgcgcccgag gtcgtggcgc gtcttgcctt cggcctcgag ctggcgctcg
     1801 accacgatct gcgtggcgat gccggcgtgg tcggtgcccg ccacccagtt ggcgttgaac
     1861 ccgcgcatgc ggtggtagcg gatgagcgcg tccatcagcg tctgctggaa ggcatgcccc
     1921 atgtgcagcg tgccggtgac gttgggcggc ggcagctgga tggtgtaggc gggccgctgc
     1981 tctttgccgg cttgggcggc gcccgccttg aaatagccgg ccgcttccca gcgctgatac
     2041 cagcgctgct cgacggcagc cgggctgaag ctcttgtcga tcactgcgcg tcggacttga
     2101 gcgcgttcac catcgaggtg gcgaccgcct cgcgcaccat ctggcgcacg ctcacggcga
     2161 gctccgcgcg caccgcgtcg agcgccttgc cgagcgcggt gtcgagcgcg cgggtcagat
     2221 tttcgttcac cacgcgctcg agctccggcc cgaggcgcgc caatactgcg cgctcgacct
     2281 cgagcgcgat gcgctcgagg acagcgcgat cgatcccggt gccgcggcgg gcgagctcgt
     2341 cggccaccac ctcggtgagc accggcacgt cgcccggcgc gcgctcgggc ttggcgcgct
     2401 tgagcagcgc atcgacgcgc tcgcccagcg ggttgttttc gcggttttcc tcgtcactca
     2461 tggctgccgg ccaggtcgtg gttcgcgatc tggtagccgc ggtcgaggta gaagcggtag
     2521 cggctgcgcc ccgccgcgcg ctcggcctcg tcggcgccga ctacctcgaa caggcgctcg
     2581 aagcgcgcga agtgcggcgg gcattcgccg gacaggttga gcagcagccc gcaatccgcc
     2641 ggcgtctctt cgccggtggc gatcaggacc ggcgtctgcg cggcgagctc gtcgcccgcg
     2701 gcgcagtgcg gcaggaagcc gatcgcctgc caggtccaca gcagcttgtc gatgcggctc
     2761 gccgtgtcgg cctgcggcgc gtagatcagc atgcgcttgc cctgggctgc ggccttgctg
     2821 gcgagacggc aggcgacctg caggcggtct tcggcgttga aatagaaatc tatggtggtc
     2881 acttgcgtgc ggcgtgacgc agcgcgaatc tcacgagaag cggaaccggc cgcccggtcg
     2941 agcccttctc gcggccgctc ttccaggcgg tgccggcgac gtcgaggtgc gcccagcgct
     3001 gcttgcgcgt gaagcgcgcg aggtagcacg ccgcggtgat ggcgccgccc ggccggccgc
     3061 cgatgttggc gaagtcggcg aagttcgagc gcagcccctc ctggtactcc tcccacagcg
     3121 gcaggcgcca gacgcggtcg gacgcgtcgt cggcggccgc caccagctcc gcggcgagcg
     3181 cctcgtcgtt ggcgaacagg ccgctcgcga cgtggccgag cgcgaccacg caggcgccgg
     3241 tgagcgtggc gatgtcgacg accttctccg ggttgaagcg cgccgcgtag gtgagcgcgt
     3301 cgcacaggat cagccgcccc tcggcgtcgg tgttgaggat ctccacggtc tgcccggaga
     3361 gcgtggtgac gatgtcgccg gggcgcgtgg cgtgcccgcc cggcatgttc tcgcaggtcg
     3421 ggatcacgcc gaccacgttc accggcgcct tcatcccggc gagcgcgcgg atggcgccga
     3481 gaaccgagcc ggcccccgac atgtcgaact tcatctcgtc catctcgccc gccggcttga
     3541 gcgaaatgcc gccggtgtcg aaggtgatgc ccttgccgac cagcgctatg ggctttcgcc
     3601 ccttcgccgc gccgttgtag cgcagcacga tcagcttggg cgggtggtgg gaagcgctcg
     3661 ccaccgcgag cagcgcgccc atgccgagct tctgcatgtc ggcgcgctcg agcacctcga
     3721 cgccgagctt gtactgcttg gccagcttct tcgcctcctc ggcgaggtag gcgggggtgc
     3781 agatgttcgg cggcaggttg ccgagcgtct tggcgagatc gatgccgtcg gcggtcgccg
     3841 cggcctcggc gagggtctgt tggagctctt ttttcgcagt gaaaggaaaa atcacgtgct
     3901 gcagggccgc cgccggcgcc ttcttctgcg acttgagctg gtcgaagcga tagaaggctt
     3961 cgcgcaagcc gagcaccgcg tggcgcaggt tccaggtcag atgccgctcg ccgaccttca
     4021 tgtcgacgag gaagaaggcg gcgtcctttg cgcccaggtc cttgagcgcg ttggcagcgc
     4081 cgcgcaccgc gtcgcggaaa gcgctctcgc cgaactcgcc gcgctcgccc aggctgacga
     4141 gcagcacgcg ctcggcggcg atgcccgcca cgccccgcag cagcaggctc gagccggcct
     4201 tgccggagag gtcggcgagc gccttgcgca gcgcgccctt cgcggcctgg tcggcgcgcc
     4261 gggcggcggg ggtgagctct tggttggcgt aggtaccgag gacgacgcag cccgctttcg
     4321 ccgtctcggg cgagagcgtc tttatgctaa attccatggt gaaagtatcg catgacacaa
     4381 cgaagttttc ctcaaggggc gaagttttcc taggtgacat cgcctaagtg atcttccagc
     4441 gcgcgctgct cagggaattc gccggcctgg cgggcgcggt gttcatgacg ctgttcgcga
     4501 ttgcgctcac cacgcgcctg atccgcctgc tcggccaggc ggccggcggc aagattccct
     4561 ccgacgcggt gatcgccttc ctcggcttct tcgcgctcgg cacgctgccg gtgctgctct
     4621 cgctcaccat gttcatctcg gtgctgatga cgctgacgcg cagctggcgc gactcggaga
     4681 tggtgatctg gttcggctcg ggcctttcgc tcaccgcgtg gctcaagccg gtgctgatct
     4741 tcgcgctgcc gcagatcgcg gtgatcacgg cgctgtcgat cttcatctcg ccgtgggcgg
     4801 cgcagatggg tgcgcagtac gcgacgcgcc tggagacgcg cgacgacgtc tcgcgcgtca
     4861 gcccgggcgt gttcggcgag accgccaatc gcgagcgcgt gttcttcgtc gagtcggtgt
     4921 cgggcgacgc agccgcggtg cagaacgtgt tcatcagctc gagccaccag tcgaagagcg
     4981 gcgtcagcat gagccgcagc gggcacaccg aggcggcgga gaacggcgac cgcttcatcg
     5041 tgctcgagca gggccggcgc tacgaaggcg cgcccggcga cgagcagtac cgcatcaccg
     5101 agttcgagcg ctatgccgcg cgcatcgaga ccaaggaggg caaggagccg gcggtgaccc
     5161 accggacgct gcccacctgg gcgctgatca tcaacccgac caaccagaac ctgggcgagc
     5221 tgctgtggcg cttcggcatc ccggtgtcgg cgctggtgct ggtgctgatg gcgatcccga
     5281 tgagcttcgt caacccgcgc gccgggcgct cgatcaacct gatgatcgcg ctgctcacct
     5341 acatcgtgta ttccaacctg ctgtcggtca gcgtggcgcg cgtcgcgcaa ggccgcatgg
     5401 acttcgcgct aggctcgtgg ctggtgcacg cggcgatggt gctgctgctg ctgttcatgt
     5461 tcgcccagcg catgcagatc atccggctgc gcctcgcgcg ctgacactgc gatgcgcact
     5521 ctagagcgct atcttgg
//
LOCUS       DASYJA010000530         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3076850, whole genome shotgun sequence.
ACCESSION   DASYJA010000530 DASYJA010000000
VERSION     DASYJA010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3076850"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1556
                     /locus_tag="VGO02_11460"
     CDS             <1..>1556
                     /locus_tag="VGO02_11460"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081256.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEV7478340.1"
                     /translation="SGRSVIVVGPQLKLHQCGLPKKMALELFKPFIFNKLELLGLATT
                     IKAAKRLVEQEVAEVWDILEEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLHP
                     LVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDIVLG
                     LYYATRERINAKGEGMSFADLAEVHRAYDSRQVELNARVWVRLKEKLLSETGEVSEKI
                     TRYETTVGRALLSEILPLGLSFAQLNKPLKKKEISRLINIGFRLCGLRETVIFADKLM
                     QAGFSIATRAGISFGVHDMSIPPQKQELIKAAETEVKEIEVQYTSGLVTAGERYNKVV
                     DIWSRCGEQVGKVMMEQLGQEEVENRHGKKVKQEAFNSIYMMADSGARGSAAQIRQLA
                     GMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTRR
                     LVDVTQDLVVTEDDCGTQNGVSMKALVEGGEVVEALKERILGRTLATDLIHPETQDVL
                     YPAGTLIDED"
BASE COUNT          304 a          509 c          510 g          233 t
ORIGIN      
        1 ctcgggccgc tcggtcatcg tggtcggccc gcagctcaag ctgcaccagt gcggcctgcc
       61 gaagaagatg gcgctcgagc tgttcaagcc gttcatcttc aacaagctgg agctgctcgg
      121 cctcgccacc accatcaagg cggccaagcg cctggtggag caggaagtcg ccgaagtgtg
      181 ggacatcctg gaagaagtga tccgcgagca cccggtgctg ctcaaccgcg cgccgacgct
      241 gcaccgcctg ggcatccagg ccttcgagcc ggtgctgatc gagggcaaag cgatccagct
      301 gcatccgctg gtgtgcgcgg cgttcaacgc cgacttcgac ggcgaccaga tggcggtgca
      361 cgtgccgctc tcgctggaag cgcagatgga agcgcgcacg ctgatgctcg cctccaacaa
      421 cgtcctgtcg ccggcgaacg gcgagccgat catcgttccg tcgcaggaca tcgtgctcgg
      481 cctgtactac gccacgcgcg aaaggatcaa tgcgaaaggc gagggcatga gcttcgccga
      541 cctcgcggaa gtgcatcgcg cctacgactc gcgccaagtc gagctcaatg cccgcgtctg
      601 ggtgcgcctc aaggagaagc tgctctccga aacgggcgag gtgagcgaga aaatcacgcg
      661 ctatgagaca accgtcggcc gggcgctgct gtccgagatc ctgcctctcg gcctgtcctt
      721 cgcgcagctc aacaagccgc tgaagaagaa ggagatctcg cgcctgatca acatcggctt
      781 ccgcctctgc ggcctgcgcg agaccgtgat cttcgccgac aagctgatgc aggcgggctt
      841 cagcatcgcc acgcgcgccg gcatctcgtt cggcgtgcac gacatgtcga ttccgccgca
      901 gaagcaggag ctcatcaagg ccgccgagac cgaggtcaag gaaatcgagg tgcagtacac
      961 ctcgggcctg gtgaccgccg gcgagcgcta caacaaggtg gtcgacatct ggtcgcgctg
     1021 cggcgagcag gtcggaaaag tcatgatgga gcagctcggt caggaagagg tcgagaaccg
     1081 ccacggcaag aaggtgaagc aggaggcgtt caactccatc tacatgatgg ccgactccgg
     1141 cgcgcgcggc tcggcggcgc agatccggca gctcgccggc atgcgcggcc tgatggccaa
     1201 gcccgacggc tcgatcatcg agacgccgat caccgcgaac ttccgcgaag gcctgaacgt
     1261 gctgcagtac ttcatctcca cgcacggcgc gcgcaagggt ctcgccgaca ccgcgctcaa
     1321 gaccgccaac tccggctacc tgacgcggcg cctggtggac gtgacgcagg acctggtggt
     1381 caccgaggac gactgcggca cgcagaacgg cgtttccatg aaggccctgg tcgagggcgg
     1441 cgaagtggtc gaggcgctca aggagcgcat cctcgggcgc acgctggcca ccgatctcat
     1501 ccatcccgag acgcaggacg tgctgtatcc ggcagggacc ctgatcgacg aggacg
//
LOCUS       DASYJA010000531         2259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2167828, whole genome shotgun sequence.
ACCESSION   DASYJA010000531 DASYJA010000000
VERSION     DASYJA010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2167828"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1338
                     /gene="carB"
                     /locus_tag="VGO02_11465"
     CDS             <1..1338
                     /gene="carB"
                     /locus_tag="VGO02_11465"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV7478341.1"
                     /translation="VQYGGQTPLKLARDLAAAGVPIIGTSADSIDIAEDRERFQKLIE
                     KLKLRQPPNRTARSPEEALKLAEEIGYPVVVRPSYVLGGRAMEIVHFQSDLERYMREA
                     VKVSNDSPVLLDRFLDDAIEVDVDCVCDGKDVLIGGIMEHVEQAGVHSGDSACCLPPN
                     SLASHVERDLRRQTAQMARALKVKGLMNVQYAIQRDTVYVLEVNPRASRTVPFVSKAT
                     GVALAKVAARCMAGKSLKEQGVEEVTPPYFSVKEAVFPFAKFPGVDTILGPEMKSTGE
                     VMGVGETFGEAFVKSQLAAGVKLPSGGKAFLSVRDPDKVAAVQIARDLIALGFSLLAT
                     RGTAAVLKSHGIPVAQVNKVAEGRPHIVDMIKNGDVTFIVTTVEATRTAVSDSRAIRT
                     TALAKRVTYYTTVAGGKAACAGMKHLSHLEPYVLQELHARLRLPSSEPSPVIS"
     gene            1355..1810
                     /gene="greA"
                     /locus_tag="VGO02_11470"
     CDS             1355..1810
                     /gene="greA"
                     /locus_tag="VGO02_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV7478342.1"
                     /translation="MTKRGADLLREELHRLKTIERPANSTAIGEARAHGDLSENAEYH
                     AARERAGFIEGRISELEAKLGNAQVIDPKLVDAGGRCVFGSTVDVESGGETFTWQIVG
                     EDEADIKKGRIYVSSPIARALIGKEPGEVVEVQTPGGIKRYEIIDVRYE"
     gene            complement(1834..>2259)
                     /locus_tag="VGO02_11475"
     CDS             complement(1834..>2259)
                     /locus_tag="VGO02_11475"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF003559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEV7478343.1"
                     /translation="RLLGVDKRKRIVLIAHDRLKPALVEWARGHIELLRQHELYATGT
                     TGARIEKELALKVSRFLSGPKGGDQQVGARIAERALDLMIFFWDPLEPQPHDVDVKAL
                     LRIAVLYDIPIANNPASADFLISSPLMGKTYERENPDYS"
BASE COUNT          389 a          760 c          759 g          351 t
ORIGIN      
        1 gtgcagtacg gcgggcagac gccgctcaag ctcgcgcgcg acctggccgc cgccggcgtg
       61 ccgatcatcg gcaccagcgc cgactcgatc gacatcgccg aggaccgcga gcgcttccag
      121 aagctgatcg agaagctgaa gctgcgccag ccgcccaacc gcaccgcgcg ctcgccggaa
      181 gaggcgctca agctggccga ggagatcggc tacccggtgg tggtgcgccc gagctacgtg
      241 ctcggcggcc gcgccatgga gatcgtgcat ttccagtccg acctcgagcg ctacatgcgc
      301 gaggcggtga aggtgtccaa cgactcgccg gtgctgctcg accgcttcct cgacgacgcc
      361 atcgaggtcg acgtcgactg cgtgtgcgac ggcaaggacg tgctgatcgg cggcatcatg
      421 gagcacgtcg agcaggccgg tgtgcactcg ggcgactcgg cgtgctgcct gccgcccaac
      481 tcgctcgcca gccacgtcga gcgcgacctg cgccgccaga ccgcgcagat ggcgcgcgcc
      541 ctgaaggtga agggcctgat gaacgtgcag tacgcgatcc agcgcgacac ggtctacgtg
      601 ctggaggtga acccgcgcgc ttcgcgcacc gtgcccttcg tctccaaggc gaccggcgtc
      661 gcgctcgcca aggtcgcggc gcgctgcatg gcgggcaagt cgctcaagga gcagggggtc
      721 gaggaagtca cgccgcccta cttctcggtg aaggaggcgg tgttcccctt cgccaagttc
      781 cccggcgtcg acaccatcct gggccccgag atgaagtcga ccggcgaggt gatgggggtg
      841 ggcgagacct tcggcgaagc gttcgtcaag tcgcagctcg ccgccggcgt gaagctgcct
      901 tcgggcggca aggcgtttct cagcgtgcgc gaccccgaca aggtggcggc cgtgcagatc
      961 gcgcgcgacc tgatcgccct gggcttctcc ctgctggcga cgcgcggcac ggcggcggtg
     1021 cttaagtccc acggcattcc ggtggcgcag gtgaacaagg tggccgaggg ccggccgcac
     1081 atcgtcgaca tgatcaagaa cggcgacgtg accttcatcg tcaccaccgt ggaagccacc
     1141 cgcaccgcgg tctcggactc gcgcgccatc cgcaccacgg cgctggcgaa acgcgtgacc
     1201 tattacacga cggtggccgg cggcaaggcc gcctgcgccg gcatgaagca cctttcgcac
     1261 ctcgagccgt acgtgctgca ggagctgcat gcgagactac gccttccttc ctccgaaccc
     1321 tcaccggtaa tctcctgacc ctatgaaggt tcccatgacc aagcgcggcg ccgatctgct
     1381 gcgcgaagag ctgcaccgcc tgaagacgat cgagcgtccg gccaactcca cggcgatcgg
     1441 cgaggcgcgc gcgcatggcg acctctccga gaacgccgag taccacgccg cgcgggagcg
     1501 cgccggcttc atcgagggac gcatctccga gctcgaagcc aagctcggca acgcgcaggt
     1561 gatcgacccg aagctggtcg acgccggcgg ccgctgcgtg ttcggctcca ccgtcgacgt
     1621 cgagagcggc ggcgagacct tcacctggca gatcgtcggc gaggacgaag ccgacatcaa
     1681 gaaaggccgc atctatgtca gctcgccgat cgcgcgcgcg ctgatcggca aggagcccgg
     1741 agaggtggtc gaagtacaga cccccggcgg catcaagcgc tacgagatca tcgacgtccg
     1801 ctacgaataa tcggtgtcag acaccgattg ggtttacgag tagtcggggt tttccctctc
     1861 gtaagtcttg cccatcagtg gtgaggaaat caggaagtcc gccgaagcag ggttgttggc
     1921 gatagggatg tcgtagagca ccgcgatgcg tagcagcgcc ttcacatcga cgtcgtgcgg
     1981 ctgcggctcg agcggatccc agaagaagat catcaggtcg agggcgcgct cggcgatcct
     2041 ggcgccgacc tgctggtcgc cgcctttcgg accgctcagg aaacggctta ccttgagtgc
     2101 gagctccttc tcgatcctcg ccccggtggt gccggtggcg tagagctcat gctgccgtag
     2161 cagctcgatg tggccgcgtg cccattccac cagcgccggc ttcagccggt cgtgcgcgat
     2221 caggacgatg cgcttgcgct tgtccacgcc tagaagccg
//
LOCUS       DASYJA010000532         2272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_899446, whole genome shotgun sequence.
ACCESSION   DASYJA010000532 DASYJA010000000
VERSION     DASYJA010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899446"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VGO02_11480"
     CDS             <1..577
                     /locus_tag="VGO02_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011901958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEV7478344.1"
                     /translation="IASGGLSGLEDVQAICEKLQIQLGGGIRDLDTIESYIDAGVALV
                     VIGTAAVKNPGFLSDAAYAFPGHIIAGLDAKDGKVAVEGWSKMTGHDVVDLAKKYEEF
                     GIEALIYTDIGRDGMLSGVNIEATLKLAQAIKTPIIASGGLSSIKDVEAVCKLVPEGI
                     IGAIAGRALYEGKLDLKKAQAAADQATAKKA"
     gene            579..1337
                     /gene="hisF"
                     /locus_tag="VGO02_11485"
     CDS             579..1337
                     /gene="hisF"
                     /locus_tag="VGO02_11485"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012362933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEV7478345.1"
                     /translation="MGLAKRVIPCLDVTAGRVVKGVRFLELRDAGDPVEIAARYDAEG
                     ADELTFLDITASSDDRDILLHVIEAVASKVFIPLTVGGGVRKVEDVRRLLNAGADKVS
                     INTSAVTNPKLVHEASDIVGNQAIVVAIDAKKKGASWEVFTHGGRNPTGLDAIDWARR
                     MVEAGAGEILLTSMDRDGTRHGFDLALTRAVSEAVSVPVIASGGVGTKEHLADGVLEG
                     RADAVLAASVFHFGDFTVRQAKEHMRSRGIEVRL"
     gene            1334..1717
                     /gene="hisI"
                     /locus_tag="VGO02_11490"
     CDS             1334..1717
                     /gene="hisI"
                     /locus_tag="VGO02_11490"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEV7478346.1"
                     /translation="MSWLDEVPWNEHDLVAVVTQDFTSGKILTVAWMNREALQQTAEK
                     KEAVYYSRSKKRLWRKGEESGYIQKVREIRIDCDADAVLLKVEQVGGIACHTGHESCF
                     FRKLENGKWVEMDPVLKDPKLIYKK"
     gene            1714..2031
                     /locus_tag="VGO02_11495"
     CDS             1714..2031
                     /locus_tag="VGO02_11495"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEV7478347.1"
                     /translation="MKDILDRIAASIEERKDGDPEKSYVAKLFGKGDDAMLKKVGEEA
                     TEVVLAAKSGDRLHLVREVTDLWFHCMIVLARHGLGPKDVLAEMQRREGISGLDEKAS
                     RKK"
     gene            2028..>2272
                     /locus_tag="VGO02_11500"
     CDS             2028..>2272
                     /locus_tag="VGO02_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEV7478348.1"
                     /translation="MTTDPNCIFCKIVRGEIPAKKVHEDEDVLAFHDVRPQAPVHFLI
                     VPKEHVPTLYDAGMKQHKALGKMLALVGELARKEGAA"
BASE COUNT          457 a          715 c          771 g          329 t
ORIGIN      
        1 catcgcctcc ggggggctga gcggcctgga ggacgtgcag gccatctgcg agaagctgca
       61 gatccagctc ggcggcggca tccgcgacct cgataccatc gaaagctaca tcgacgccgg
      121 cgtcgcgctg gtggtgatcg gcacagcggc ggtgaagaac cccggcttcc tgtccgacgc
      181 cgcctacgcc ttccccggcc acatcatcgc cggcctcgac gcgaaggacg gcaaggtggc
      241 ggtcgagggc tggtcgaaga tgaccggcca cgacgtggtc gacctggcga agaagtacga
      301 ggagttcggc atcgaggcgc tgatctacac cgacatcggc cgcgacggca tgctgagcgg
      361 cgtcaacatc gaggccacgc tcaagctcgc ccaggcgatc aagacgccga tcatcgcctc
      421 cggcggcctg tccagcatca aggacgtcga ggcggtgtgc aagctggtgc ccgagggcat
      481 catcggcgcc atcgccgggc gcgcgctcta cgagggaaag ctcgacctga agaaagccca
      541 ggccgccgcc gaccaggcga ccgccaagaa ggcatagcat gggcctcgcc aagcgggtca
      601 ttccctgcct cgacgtcacc gcgggccggg tggtgaaggg cgtcaggttc ctcgagctgc
      661 gcgacgccgg cgatccggtc gagatcgccg cgcgctacga cgccgaaggc gccgacgagc
      721 tcaccttcct cgacatcacc gccagctcag acgaccgcga catcctgctg cacgtcatcg
      781 aggccgtggc gagcaaggtg ttcatcccgc tcaccgtcgg cggcggcgtg cgcaaggtcg
      841 aggacgtgcg ccgcctgctg aacgccggcg ccgacaaggt gtcgatcaat acttccgcgg
      901 tcaccaaccc gaaactcgtg cacgaagcgt ccgacatcgt cggcaaccag gcgatcgtcg
      961 ttgccatcga cgccaagaag aagggcgctt cctgggaagt cttcacgcat ggcgggcgca
     1021 acccgaccgg cctggacgcg atcgactggg cgaggcggat ggtcgaggcg ggcgccggcg
     1081 agatcctgct caccagcatg gaccgcgacg gcacgcgcca cggcttcgac ctcgccctga
     1141 cgcgcgccgt gtctgaggcg gtgagcgtgc ccgtaatcgc ctcgggcggc gtcggcacca
     1201 aggagcacct cgccgacggc gtgctcgaag gccgcgccga cgcggtgctc gccgcaagcg
     1261 tgttccactt cggcgacttc accgtgcgcc aggcgaagga gcacatgaga agccgcggca
     1321 tcgaggtgcg cctgtgagct ggctcgacga agtaccgtgg aatgagcatg acctggttgc
     1381 ggtcgtgact caggacttta cttccggaaa aatcctcacc gtggcctgga tgaaccgcga
     1441 ggcgctgcag cagaccgccg agaagaagga ggcggtctat tactcgcgct cgaagaaaag
     1501 gctttggcgc aagggcgaag aatccggcta catccagaag gtcagggaga tacgtattga
     1561 ttgcgatgcc gacgcggtgc tgctcaaggt cgagcaggtc ggcggcatcg cttgtcacac
     1621 gggacatgag agctgcttct tccgcaaact cgagaacggg aaatgggtgg agatggatcc
     1681 ggtgctgaag gatcccaagc tcatctacaa gaaatgaaag acatcctcga caggattgcg
     1741 gccagcatcg aagagcgcaa agatggcgac ccggagaagt cctacgtcgc caagctcttc
     1801 ggcaagggcg acgacgccat gctgaagaag gtcggcgaag aggcgaccga ggtggtgctc
     1861 gcggcgaaga gcggcgaccg gctgcacctc gtgcgcgagg tcaccgacct gtggttccat
     1921 tgcatgatcg tgctcgcgcg ccacggcctc gggccgaagg acgtgctcgc cgagatgcag
     1981 cggcgcgaag gtatctccgg actggacgaa aaggcatcgc gcaagaaatg acgaccgacc
     2041 caaactgcat cttctgcaag atcgtgcgcg gcgagatccc ggccaagaag gttcatgagg
     2101 acgaggatgt gttggcattc cacgacgtcc gcccgcaggc gccggtgcac ttcctgattg
     2161 tccccaagga gcatgtgccc accctctatg acgcggggat gaagcagcac aaggcgctgg
     2221 gaaagatgct cgcgctggta ggcgagctcg cgcgcaagga gggggccgcc ga
//
LOCUS       DASYJA010000533         4886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_807521, whole genome shotgun sequence.
ACCESSION   DASYJA010000533 DASYJA010000000
VERSION     DASYJA010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807521"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VGO02_11505"
     CDS             <1..640
                     /locus_tag="VGO02_11505"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEV7478349.1"
                     /translation="FAATLLAAASAQAQSTRGGKGEFYLSPVFTDGKNYSFEGGSSAK
                     TDTGFGLTLGYAFNFDQHKALGIEVAWADQDYRATVQPGPGNIANNATDIRGTLETWT
                     VRFLGNYYFMQGNFTPFVTGGLGWTYIDTNIPTGLPQNVCWYYPWYGTSCASYVPTAT
                     STKFSYNAGLGLRMEMGKGFVRAMVNEQWLDFGGSYGSSSVTQYRIDFGVKF"
     gene            653..1234
                     /locus_tag="VGO02_11510"
     CDS             653..1234
                     /locus_tag="VGO02_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478350.1"
                     /translation="MKSKAVAAALLAAFVMPAHAQPLKGGAIAASQIDAVLTVLAVDQ
                     KARTVTVRGPKGGVTTLKVPPQAQNLDQVKQGAQFRVKYVEAVAVGIRKGGTPDAIVA
                     EEVRLAPKGGTPGGVVVRTQQLAGVVDAVDYTDRYIALRGPKGNTLSLKVADGVKLEG
                     LSAGDRVAVTHTEALAMEMIPQAPAKKPAAKKS"
     gene            complement(1231..4284)
                     /locus_tag="VGO02_11515"
     CDS             complement(1231..4284)
                     /locus_tag="VGO02_11515"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEV7478351.1"
                     /translation="MRVRSSLRRRLAYGLGFAVVAFAGLLALGGWLIDTPAVKAAIQQ
                     RLSAALGGQIAWEALELRLLPAPHGELRRVRVEIPGAVSARADQVDAYLRLWPLLRGH
                     PEIASLSVSRPEVRIAEPGGAQKEDAPIDPLAAYRRAIEPVAQALQKFAPDTDFRIES
                     AAIDLAGMGLELRDLNVALRTTRDGAELDLDASSNFWKRLRVRGRVEYADLSAHGTLR
                     VDEVALHKDLPAATLSAQLRTDAKTAIECDLEAALGSLLPEAKAKLSLPAGGKPAEVD
                     ARLSRIDLVQAVAFARRNVSGLEVIESVEGRLSANVRLSLGEPWRASVDVFESDASAR
                     LAGLPWKLSAHAAQIAASEKQVDVRGLRGTLGGSSFSEVAARIELGKQPRLSAASGRA
                     TLQLEQWFPWLQKQLPLEEINALTGNVEVALQRMALRFDRSAEVSFDAVATPRQVSAT
                     LKALPAPVSVTGGSLQVSAAQVRMDRLAVAMLDASALVSGTVTTKGPKLELALAEGAL
                     GEKIVQWALARAEVPARLEPRTPLRFAAKRIAWAPQAPLEADASIDFDGGPAVAAVLA
                     WQPDKLELRRLAIKDARSDAVLGATIGGDVLQASFSGTLYGRSLASMLRKPRAGVQGD
                     LGVARGKLVLKGHRKAPEQSTAEGRLQVEALDLSQLVGAKVLLQSGDFSADGESLRIA
                     QARLEWEDQSVSLNGVLRRTAQGPVIDARLESPGVVVERLLAAEKSATAGEPKQSKLW
                     PLPVTGQVDVRAGFVQYQHYKIAPLEGSLKLERERASLQVKEARMCGVSFPLTSEALP
                     DSFAFATQITMKDQPIEQAMRCLTGETVEITGNADLRADLRSRGKVEDLIRNLTGTAQ
                     ADLRKGTIKKFALIGNILSVLSLRNLVSPQSAKEGAFAYRSLSAKGRFEGGAFLIEES
                     FFDSNAARLAATGTVDLLGANSRLDVLVGLLTTVDRVAGAIPLIGDVFGGSLTALPVA
                     VSGDIRDPRVVPLGPHAVTDRLLGIFERTLKLPGKLVVPGSATTD"
     gene            4383..4754
                     /locus_tag="VGO02_11520"
     CDS             4383..4754
                     /locus_tag="VGO02_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478352.1"
                     /translation="MRLLALILAAAFASPAPAQNWIGLLKNTPAERFDEEDLRLFLDT
                     SRKALNEAPEKQTLTWENPKTRAGGDITVLRSFESKGRPCKEVRVRNHAQGRKGDNKL
                     SLCKVDEKWRLLSASQLGKAK"
BASE COUNT          797 a         1726 c         1646 g          717 t
ORIGIN      
        1 ctttgccgct acgctgcttg ccgcggcatc cgcccaggcg cagtcgacgc gcggcggcaa
       61 gggcgagttc tacctgtcgc cggtgttcac cgacggtaag aactattcat tcgaaggcgg
      121 ctccagcgca aagaccgaca cgggcttcgg cctgacgctc ggctacgcgt tcaacttcga
      181 ccagcacaag gccctcggca tcgaggtggc atgggccgac caggactacc gcgcgaccgt
      241 ccaacccgga cccgggaaca ttgccaacaa cgccaccgac attcgcggca ctctggaaac
      301 ctggaccgtg cgcttcctcg gcaactacta cttcatgcag ggcaacttca ccccgttcgt
      361 cacgggaggc ctcggctgga cctacatcga cacgaacatt ccgaccgggc tgccgcagaa
      421 cgtgtgctgg tactaccctt ggtacggcac gtcgtgcgcg tcgtacgtgc ctacggcgac
      481 ctcgaccaag ttcagctaca acgcgggcct cggcctgcga atggagatgg gcaagggctt
      541 cgtgcgcgcc atggtgaacg agcagtggct cgacttcggc ggctcgtacg gctcctcgtc
      601 ggtgacgcag taccgcatcg acttcggcgt caagttctaa aggagccggc acatgaaaag
      661 caaggccgtc gcagcagccc ttcttgctgc cttcgtcatg ccggcgcacg cgcagccgct
      721 caagggcggc gccatcgccg ccagccagat cgacgcggtc ctgacggtgc tggcggtcga
      781 ccagaaggcg cgcactgtca ccgtgcgcgg gcccaagggc ggggtgacga cgctcaaggt
      841 gccgccgcag gcgcagaacc tcgaccaggt gaagcagggc gcgcaattca gggtgaagta
      901 cgtcgaggcg gtcgcggtcg gcatccgcaa gggcggcacg cccgacgcga tcgtggcgga
      961 agaagtgagg ctcgctccga aaggcggcac gcccggcggc gtcgtggtgc gcacgcagca
     1021 gcttgccggc gtcgtcgacg cagtcgacta caccgatcgc tacatcgcgc tgcgcggacc
     1081 gaaaggcaac acgctctcgc tcaaggtggc cgacggcgtg aagctcgagg ggctgagcgc
     1141 cggtgaccgc gtcgccgtca cgcataccga ggcgctggcg atggagatga tcccgcaggc
     1201 gccggcgaag aagccggcgg cgaagaagag ctagtccgtc gtcgccgagc ccggcaccac
     1261 cagcttgccc ggcagcttca gcgtgcgctc gaagatgccg agcaggcggt cggtcacggc
     1321 gtgcgggccc agcggcacga cgcgcgggtc gcggatgtcg ccgctcaccg cgaccggcag
     1381 cgcggtcagc gagccgccga acacgtcgcc aatcagtggg atcgcgccgg cgacgcggtc
     1441 gacggtggtg agcaggccga ccagcacgtc gaggcgggaa ttggcgccga gcaggtccac
     1501 cgttccggtg gcggcgagcc gcgccgcgtt gctgtcgaag aagctctcct cgatgaggaa
     1561 cgcgccgccc tcgaagcgcc ccttcgcgct caagctgcgg taggcaaacg cgccctcctt
     1621 cgccgactgc ggtgaaacga ggttgcgcaa cgagagcacg gaaagaatgt tgccgatcaa
     1681 ggcgaacttc ttgatggtgc ccttgcgcag gtcggcttgc gccgtgccgg tgagattgcg
     1741 gatcaggtcc tcgactttgc cacgcgagcg caggtcggcg cgcagatccg cgttgccggt
     1801 gatttccacc gtctcgccgg tcaggcagcg catcgcctgc tctatcggct ggtccttcat
     1861 cgtgatctgc gtcgcgaacg cgaagctgtc gggcagtgcc tcgctcgtga gcggaaacga
     1921 cacgccgcac atgcgcgctt ccttcacctg caggctggcg cgctcgcgct ccagcttcag
     1981 gctgccttcg agcggcgcga tcttgtagtg ctggtactgg acgaagccgg cgcgcacgtc
     2041 gacctgcccg gtcaccggca gcggccacaa cttcgactgc ttcggctcgc cggcggtggc
     2101 ggacttctcg gcagccagca gccgctcgac cacgaccccc ggtgattcga gccgcgcgtc
     2161 gatcaccggc ccttgcgcgg tgcggcgaag cacgccgttc aggctgacgg actggtcctc
     2221 ccactcgagc ctcgcctggg cgatgcgcag gctctcgcca tcggcgctga aatcgccgct
     2281 ctgcaggagc accttggcgc cgaccagctg gctgagatcg agcgcctcga cctgcagccg
     2341 gccctcggcg gtgctctgct ccggcgcctt gcggtggccc ttcagcacaa gcttgccgcg
     2401 cgcgacgccc aggtcgcctt gcacgccagc gcgcggcttg cgcagcatcg acgcaaggct
     2461 gcggccgtag agcgtccccg agaagctcgc ctgcagcaca tcaccgccga tggtggcgcc
     2521 gagcacggcg tcgctgcgcg cgtccttgat cgccaggcgg cgcagctcga gcttgtcggg
     2581 ctgccatgcc aggaccgcgg cgacggcagg gccgccgtcg aagtcgatcg aagcgtcggc
     2641 ctccagcggc gcctgcggcg cccaggcgat gcgcttcgcg gcgaagcgca gcggcgtcct
     2701 cggctccagg cgcgccggca cctcggcgcg cgccagagcc cattgcacga tcttctcgcc
     2761 cagcgcgcct tccgcgagcg cgagctcgag cttcggcccc ttcgtcgtca cggttcccga
     2821 aaccagcgcg ctggcgtcga gcatcgcgac cgccagccgg tccatccgca cctgggccgc
     2881 gctgacctgc agcgagcctc ctgtcacgct gaccggcgca ggcagcgcct tcagcgtcgc
     2941 gctcacctgg cgcggcgtcg cgaccgcgtc gaaggagacc tcggcggaac ggtcgaagcg
     3001 cagcgccatg cgctgcagcg cgacctcgac gttgcccgtc agcgcattga tctcctccag
     3061 cggcagctgc ttctgcagcc agggaaacca ctgctcgagc tgcagcgtcg cgcggccgga
     3121 tgccgccgag aggcgcggct gcttgccgag ctcgatgcgc gccgcgacct ccgagaagct
     3181 ggatccgccg agcgtaccgc gcaagcctct gacgtcgacc tgcttttccg aggccgcgat
     3241 ctgcgccgcg tgcgccgaca gcttccacgg cagccctgcc agcctggccg acgcgtcgga
     3301 ctcgaagaca tcgacgctcg cccgccatgg ctcgccgagg gaaagacgga cattcgccga
     3361 caggcggcct tcgaccgact cgatcacctc gaggccgctc acattgcgcc gcgcgaaagc
     3421 aaccgcctgc acaagatcga tgcgcgacag ccgggcatcc acctcggcgg gcttgccccc
     3481 ggcgggcagc gagagcttcg ccttggcctc gggcagcagc gagccgagcg cggcctcgag
     3541 gtcgcattcg atcgcagtct tcgcgtcggt gcgcagctgt gcgctaagcg tcgcggccgg
     3601 caggtccttg tgcagcgcca cctcgtcgac ccgcaacgtg ccgtgcgccg acaggtcggc
     3661 gtactcgacg cggccgcgta cgcgcaggcg cttccagaag ttgctcgacg cgtcgaggtc
     3721 gagctccgcg ccgtcgcgag tggtgcgcag cgcgacgttc aggtcgcgca gctcgaggcc
     3781 catccccgcc aggtcgatcg ccgccgactc gatcctgaag tcggtgtcgg gggcgaactt
     3841 ttgcagcgcc tgcgccaccg gctcgatcgc ccgccgatag gcggcgagcg ggtcgatcgg
     3901 agcgtcctcc ttttgcgcgc cgccgggctc ggcgatgcgc acttcgggac gcgataccga
     3961 cagggaggcg atttcggggt ggccgcgcag aagcggccag agccgcagat aggcatcgac
     4021 ctgatcggcg cgcgcgctta ccgcgccggg gatctcgacg cgtacgcggc gcagctcgcc
     4081 gtggggcgcc ggcaggagcc gcaactcgag cgcctcccag gcgatctggc cgccgagcgc
     4141 cgcggagagt cgctgctgga tggccgcctt gacggccggc gtgtcgatca gccagccgcc
     4201 gagcgccagc aaccccgcaa aggcaaccac agcgaagccg aggccatagg cgagccgcct
     4261 gcgcagggag gatcggacgc gcaccttgtc tgcttgatcg acgataatgt cccagcatgg
     4321 cccaagcccg cgcaagtcac aacgacggtc tgcaaaaaga ggaatccttc taggagacgt
     4381 ccatgcgttt gctcgcactg atcctcgccg cggccttcgc cagcccggct ccggcccaga
     4441 actggatcgg gctgctgaag aacacgcccg ccgagcgctt cgacgaggag gacctgcgcc
     4501 tgttcctcga cacttcgcgc aaggcgctga acgaggcgcc ggaaaagcag accctgacct
     4561 gggagaaccc caagacgcgc gccggcggag acatcacggt gctgaggagc ttcgagtcga
     4621 aaggccgccc gtgcaaggag gtgcgcgtgc gcaaccacgc gcagggccgc aagggcgaca
     4681 acaagctgag cctgtgcaaa gtcgacgaga agtggcgcct gctcagcgcg tcacagctgg
     4741 ggaaggcgaa gtagtactgc gctacttctc ctcgatcttg ccgatcttct ccagcgccac
     4801 tttcagccgc gccgcgtcgc gctcccagta ctgctggaac tcgggcgcgt cgaggtaggc
     4861 caccggcgtc tgcaccttct ccatcg
//
LOCUS       DASYJA010000534         3195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1714406, whole genome shotgun sequence.
ACCESSION   DASYJA010000534 DASYJA010000000
VERSION     DASYJA010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1714406"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1760
                     /locus_tag="VGO02_11525"
     CDS             114..1760
                     /locus_tag="VGO02_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(methylthio)propionyl-CoA ligase"
                     /protein_id="HEV7478353.1"
                     /translation="MHGLMMDVPLLISDLIRHADRHHGATEIVSKTVEDGAIHRYTYR
                     EAHARARRLANALEKLNIKPEDRVATLGWNSFRHFEIYYAVAGSGAIIHTINPRLFPD
                     QITFIANHAEDKVVFFDLSFAPLIEKLAPQLKSVKSFVCMTDRSHLPNKNLLCYEDLI
                     EAENDDYDWPSFDEKTAACLCYTSGTTGNPKGALYSHRSTMIHAYAAALPDTLNLSAR
                     EVVLPVVPMFHVNAWALPYSCALVGAKMVFPGQHLDGKSLHQLFETEGVTMSAGVPTV
                     WLGLLAYMKQQKARFSTLKSVVIGGSACPPAMIRAFQDEYGVQVLHAWGMTEMSPLGT
                     AGTLKAKQEKLGKEERYALQGKQGRAIFGVDMRIVDENGAELPWDGKAFGDLQVRGPW
                     VIKGYFKGEGGDPLVKDGENRGWFPTGDVATLDAEGYMQITDRSKDVIKSGGEWISSI
                     DLENIAIEHPAVAEAAVIGIKHPKWDERPVLVVVKKAGQEVSRDELLRFYDGKIAKWW
                     MPDDVVFVKELPHTATGKLSKLTLRQQLKDYQLRGGLDQK"
     gene            1747..2505
                     /locus_tag="VGO02_11530"
     CDS             1747..2505
                     /locus_tag="VGO02_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020831239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV7478354.1"
                     /translation="MIKSEVKDRVARVRFDRVDKKNAITPEMYAMLGKALADAEADPA
                     VRAVLLHGTADCFTAGNDVADFLNAPRTPGSSPARALFDVLPGMAKPVVAAVGGPAIG
                     IGSTMLLHCDLVYAAPNARFQLPFVPLGIVPEFGSTYLLPLLAGYQRAAELLLLGQPF
                     TAQKAYEAGIVTAVVPQENLLAEAEKAAAALAALPPESIRLTKRLMKSRHGATLAAAI
                     EEETRIFGERLHSPEAKEALSAFLEKRKPDFSRF"
     gene            2522..3142
                     /locus_tag="VGO02_11535"
     CDS             2522..3142
                     /locus_tag="VGO02_11535"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEV7478355.1"
                     /translation="MAAALVAVDVGHFNGEPGVISASGIPEFEFNLKLALQTKEELQK
                     QGLAVRMIGEKGNMIFLNHRTRAAAGADLFVSIHHDSMREQVLATKRDQLAGFSLFVS
                     RNNPQAGKSLACASAIGAELRAAGFTPSRYHADPVIGENRPFADQLNGVHFYDNLGVG
                     KTAKMPSVLVEAGVIVNRKEEARMLDPKVRARIARAIAQGVATCLR"
BASE COUNT          590 a         1035 c         1069 g          501 t
ORIGIN      
        1 cgaggcgggg cagctgcagc gcttcaacgg caaggagtgg gtgcgcttcg gcgaggtagt
       61 ggggcactag gccgcccggg cctccggggt aaacttaccc cggaggagat cctatgcacg
      121 gcctgatgat ggacgtgccg ctgctgatca gcgacctgat ccggcatgcg gaccgccacc
      181 acggcgccac cgagatcgtc tcgaagacgg tcgaggacgg cgccatccac cgctacacct
      241 accgcgaggc gcacgcacgc gcgcgccggc tggcgaatgc tctagaaaaa ctaaacataa
      301 aaccagaaga cagggtagca accctgggat ggaacagctt ccgccatttc gagatctact
      361 acgcggtggc gggcagcggg gcgatcatcc atacgatcaa cccgcggctg ttcccggacc
      421 agatcacctt catcgccaac cacgccgagg acaaggtcgt gttcttcgac ctgagcttcg
      481 cgccgctgat cgagaagctc gcgccgcagc tgaagtcggt gaagagcttc gtgtgcatga
      541 ccgatcgctc gcatcttccc aataaaaact tgctttgtta cgaagatctg atcgaggcgg
      601 aaaacgacga ctacgactgg cccagcttcg acgagaagac ggcggcctgc ctgtgctaca
      661 cctcgggcac caccggcaac ccgaagggcg ccctctactc gcaccgctcg accatgatcc
      721 acgcctacgc cgcggcgctg cccgatacgc tgaatctctc ggcgcgcgaa gtggtgctgc
      781 cggtggtgcc gatgttccac gtcaacgcct gggcgctgcc ttattcctgc gccctggtcg
      841 gcgcgaagat ggtgtttccg ggccagcacc tcgacggcaa gagcctgcac cagctgttcg
      901 agaccgaggg cgtgacgatg agcgcggggg tgccgacggt ctggctcggc ctgctcgcct
      961 acatgaagca gcagaaggcg cgcttctcga cgctgaagag cgtggtgatc ggcggctccg
     1021 cctgcccgcc ggcgatgatc cgcgccttcc aggacgaata cggcgtgcag gtgctgcacg
     1081 cttggggcat gaccgagatg agcccgcttg gcaccgccgg cacgctcaag gcgaagcagg
     1141 aaaagctcgg caaggaggag cgctacgcgc tgcaggggaa gcagggccgc gccatctttg
     1201 gcgtcgacat gcgcatcgtc gacgagaacg gcgccgagct gccctgggac ggcaaggcgt
     1261 tcggcgacct gcaggtgcgc gggccgtggg tgatcaaggg ctacttcaag ggcgagggcg
     1321 gcgacccgct ggtgaaggac ggcgagaatc gcggctggtt cccgaccggc gacgtcgcca
     1381 cgctcgatgc cgagggctac atgcagatca ccgaccgctc caaggacgtg atcaagtcgg
     1441 gcggcgaatg gatcagctcg atcgacctag agaacatcgc gatcgagcat ccggcggtcg
     1501 ccgaggcggc ggtgatcggc atcaagcacc cgaaatggga cgagcgcccg gtcctggtgg
     1561 tggtgaagaa ggcggggcag gaggtctcgc gcgacgagct gctgcgcttc tacgacggca
     1621 aaattgccaa gtggtggatg cccgacgacg tggtgttcgt gaaggagctg ccgcataccg
     1681 ccaccggcaa gctctccaag ctcacgctgc gccagcaact gaaggactac cagttaagag
     1741 gcggccttga tcaaaagtga agtgaaagac cgggtggcgc gcgttcgctt cgaccgcgtc
     1801 gacaagaaga acgccatcac tccggagatg tacgccatgc tcggcaaggc gcttgccgac
     1861 gccgaggccg atccggccgt ccgtgcagtt ctgctgcacg gtacggcaga ttgtttcacc
     1921 gcgggcaacg acgtcgccga cttcctcaat gcgccgcgca cgccgggaag ctcgccggcg
     1981 cgggcgctgt tcgacgtgct gccggggatg gccaagccgg tcgtcgccgc ggtcggcggc
     2041 ccggcgatcg gcatcggctc gaccatgctg ctgcactgcg acctggtgta tgccgcgcca
     2101 aacgcccgtt tccagttgcc cttcgtgccg ctgggcatcg tgcccgagtt cggctcgacc
     2161 tacctgctgc cgctgctcgc cggctaccag cgggccgccg agctgctgct gctcggccag
     2221 ccgttcaccg cgcagaaggc ctatgaagcc ggcatcgtga ccgccgtggt gccgcaggag
     2281 aatctgctgg ccgaggcgga gaaggccgcc gcggcgctgg ccgcgctgcc gccagaatcg
     2341 atccgcctga ccaagcgcct gatgaagtcg cgccacggcg cgacgctcgc cgcggcgatc
     2401 gaggaggaga cgcgcatctt cggcgagcgg ctgcactcgc ccgaggcgaa ggaagcgctg
     2461 agcgcgtttc tcgagaagcg caagcctgac ttctcgcgct tctagtccca tttgtagatt
     2521 cgtggctgcc gcgctggtcg cggtcgacgt cgggcacttc aacggcgagc cgggcgtgat
     2581 ctcggcgagc ggcatcccgg aattcgagtt caacctcaaa cttgctttgc aaacaaaaga
     2641 agaacttcaa aagcagggcc tggcggtgcg catgatcggc gagaagggaa acatgatctt
     2701 tctaaatcac cgcacgcgcg ccgccgccgg cgcggatctc ttcgtctcga tccaccacga
     2761 ctcgatgcgc gagcaggtgc tcgccacgaa gcgcgaccag ctcgccggct tttcgctgtt
     2821 cgtgtcgcga aacaacccgc aggccggcaa gagcctcgcc tgcgcgagcg ccatcggcgc
     2881 cgagctgcgc gccgcgggct tcaccccgtc gcgctatcat gccgacccgg tgatcggcga
     2941 gaaccgcccg ttcgccgacc agctcaacgg cgtgcacttc tacgacaacc ttggcgtggg
     3001 caagacggcg aagatgcctt cggtgctggt cgaggccggc gtgatcgtca accgcaagga
     3061 ggaggcgcgg atgctcgacc ccaaggtgcg cgcgcgcatc gcacgcgcca tcgcgcaagg
     3121 agttgcgaca tgtcttcgat aagggacctc gagcgccgtg tcggcgagga ggtcggcgtc
     3181 tcgccctggg tcgag
//
LOCUS       DASYJA010000535         2734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1532503, whole genome shotgun sequence.
ACCESSION   DASYJA010000535 DASYJA010000000
VERSION     DASYJA010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532503"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VGO02_11540"
     CDS             <1..284
                     /locus_tag="VGO02_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV7478356.1"
                     /translation="LKDDHANAQRLADGLRRAGFEVSGPSTNMVMVKVERPQELAEHL
                     KKQGILVLPRAPMRLVTHLDVDVAGIERALGAFGDYLSRSRAPARAAKA"
     gene            complement(285..851)
                     /locus_tag="VGO02_11545"
     CDS             complement(285..851)
                     /locus_tag="VGO02_11545"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HEV7478357.1"
                     /translation="MSAGHGAHADHGDHANPANKKIALLIAVLALVLAFSETLGKSAQ
                     TAALSLNIEASNLWAFFQAKTIRQTVLRAAAEQTEVTNTTPAAKKQIETWKKTAERYQ
                     SEPETNEGRKELAERAKAAEQKRDRSLAAYHHYELASAAVQIAIVLASAAIITGAVAL
                     VWIGIGLGAIGIVFCAIGFFVPTAVHLF"
     gene            complement(864..1793)
                     /locus_tag="VGO02_11550"
     CDS             complement(864..1793)
                     /locus_tag="VGO02_11550"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV7478358.1"
                     /translation="MSRDRWSLVAVVATMVVWGLNFPFVKYVLDQIGVGAFLFIRFAA
                     LPLLGLALLAIVFRGRLARTWPRREDLPRFILCALVGHTVHIAAVMSGMNLSTAFSSS
                     LVLMSGPLFTLVILAALGAERLGARQIAGTLLACAGIALFLSEKFAGGAAHAGYGDLL
                     LIVAALCFSLYTVLAQPITKRYGPLIVLSYTLLFSAPPILLLNFGAFLRIDPAKLTPL
                     VCLGMFWALVLSSFLGWMAWGWANAVRGVARCAPFNYLMPPIAGFSAWLTLGESFTWL
                     KVLGAAVTMAGVAWAQFGAGRPPPREAAQPDSA"
     gene            1823..>2734
                     /locus_tag="VGO02_11555"
     CDS             1823..>2734
                     /locus_tag="VGO02_11555"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV7478359.1"
                     /translation="MKFETPLAARAAVRSLTASKIRELYNEGIGRSDILPFWVGEPDE
                     PTPEFIRRAGIASIEAGEVFYSHNYGIPELRDALSKYLFGLHKKEIEENRLAITSAGV
                     NALMIASQLLIDPGDHVVEVVPLWPNLQEIPKILGATVHTVALRFLQSGWQLDVQELL
                     EKLKPGTKALYLNSPNNPTGWTVTREEQRTILEHCRRHGIWIFADDAYERLYFGQGRS
                     APSFLDLMEQEDRVISANTFSKSWLMTGWRLGWLVVPPALAADLGKLIEYNTSCTPIF
                     VQRAGVAAVTGGEAVISRSLQRFRSARD"
BASE COUNT          503 a          899 c          918 g          414 t
ORIGIN      
        1 ggctgaagga cgaccacgcc aatgcgcagc ggctggcgga cggcctgcgc agggccgggt
       61 tcgaggtgag cggacccagt accaatatgg tgatggtgaa ggtagagcgg ccgcaggagc
      121 ttgccgagca cctgaagaag caaggcatcc tggtcctgcc gcgcgcgccg atgcgcctgg
      181 tgacgcacct cgatgtcgac gtcgcgggga tcgagcgcgc gctcggcgcg ttcggcgatt
      241 acctcagccg gtcacgcgca ccagcccggg cggcaaaggc ctagtcagaa caggtgtacc
      301 gcggtcggca cgaaaaagcc aatggcgcag aacacgatgc cgatcgcgcc caggccgatg
      361 ccgatccaga ccagcgccac cgcgccggtg atgatcgccg ccgaggcgag cacgatcgcg
      421 atctgcaccg ccgccgaggc gagctcgtag tggtggtagg cggccagcga gcggtcacgc
      481 ttctgctccg ccgccttggc gcgctcggcg agctccttgc ggccctcgtt ggtctccggc
      541 tcggactggt agcgctccgc cgtttttttc caggtttcga tctgcttctt tgcggctgga
      601 gtcgtatttg ttacttcagt ttgctccgcc gccgccctca acaccgtctg cctgatggtc
      661 ttcgcctgga agaaagccca caggttggac gcctcgatgt tgaggctcag cgccgcggtc
      721 tgcgccgact tgccgagcgt ctccgagaac gccagcacca gggcgagcac cgcgatgagc
      781 agcgcgatct tcttgttcgc cgggttggcg tggtcgccgt gatcagcgtg agcaccgtgt
      841 cctgcggaca tgttttcccc cttttatgca gagtctggtt gcgccgcttc gcgcggcggc
      901 ggcctgccgg cgccgaactg cgcccaggcc acgcccgcca tggtaaccgc ggcgccgagc
      961 accttgagcc aggtgaagct ctcgccgagc gtcagccagg ccgaaaaccc cgcgatcggc
     1021 ggcatcaggt agttgaacgg cgcgcagcgg gcgacgccgc gcaccgcgtt ggcccagccc
     1081 caggccatcc agccgaggaa cgaggacagc accagcgccc agaacatccc caggcagacc
     1141 agcggcgtca gcttcgccgg gtcgatgcgc aggaaggcgc cgaagttgag cagcaggatg
     1201 ggcggcgccg aaaagagcag ggtgtaggag agcacgatga gcgggccgta gcgcttggtg
     1261 atcggctgcg cgagcaccgt gtagagcgag aagcatagcg cggcgacgat gaggagcagg
     1321 tcgccgtagc cggcatgcgc cgcgccgccg gcgaactttt cggagaggaa cagagcgatc
     1381 ccggcgcagg cgagcagggt gccggcgatc tgccgggcgc ccaggcgctc ggcgccgagc
     1441 gccgccagga tgacgagcgt gaagaggggc ccgctcatca gcaccagcga cgaggagaag
     1501 gcggtcgaca ggttcatgcc gctcatcacc gcggcgatgt gcaccgtgtg gccgaccagc
     1561 gcgcacagga tgaagcgcgg caggtcctcg cgcctgggcc aggtgcgcgc cagccggccg
     1621 cggaagacga tcgccagcag cgccaggccg agcagcggca gcgcggcgaa gcggatgaac
     1681 aggaacgcgc cgacgccgat ctggtcgagc acgtacttga cgaacgggaa gttgaggccc
     1741 cagacgacca tggtcgcgac cacggcaacg agggaccagc gatcgcgcga catgagcgcc
     1801 gagtttagaa tagaacccgc ccatgaaatt cgaaacaccg ctcgccgcgc gcgccgccgt
     1861 gcgctcgctg accgcttcca agatccgcga gctctacaac gagggcatcg gccgcagcga
     1921 catcctgccg ttctgggtcg gcgagcccga cgagccgacc ccggagttca tccgccgcgc
     1981 cggcatcgcc tcgatcgagg cgggcgaggt gttctacagc cacaactacg gcatcccgga
     2041 gttgcgggac gctctttcga agtacctatt tggtttacat aagaaagaga tcgaggaaaa
     2101 ccggctggcc attacctccg ccggcgtcaa tgcattgatg atcgcctcgc agctgctgat
     2161 cgatccgggc gaccacgtcg tcgaggtggt gccgctatgg cccaatctgc aggagatccc
     2221 gaagatcctg ggagcgactg tccatactgt tgccctacgg tttcttcaat cgggctggca
     2281 attggacgtt caagagctgc tcgagaaact caaaccggga acgaaagcgc tgtacctcaa
     2341 ctcgcccaac aacccgaccg gctggacggt cacgcgcgag gaacagcgca caatcctcga
     2401 gcactgccgc cgccacggca tctggatctt cgccgacgac gcctacgagc gactctattt
     2461 cgggcagggc cgctccgcgc cgtccttcct cgatctgatg gaacaggaag atcgcgtcat
     2521 cagcgccaac accttttcga agtcctggct gatgaccggc tggcgcctgg gctggctggt
     2581 ggtgccgccg gcgctggccg ccgacctcgg caagctgatc gagtacaaca cctcgtgcac
     2641 gccgatcttc gtgcagcgcg ccggcgtcgc cgctgtcacg ggcggcgaag ccgtcatctc
     2701 aagaagtctg cagcgcttca ggagcgcgcg cgat
//
LOCUS       DASYJA010000536         2617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2350546, whole genome shotgun sequence.
ACCESSION   DASYJA010000536 DASYJA010000000
VERSION     DASYJA010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2350546"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VGO02_11560"
     CDS             complement(<1..475)
                     /locus_tag="VGO02_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEV7478360.1"
                     /translation="MTSLAVHPQRNSIRLQLRQNLLLQGMSPAQWAELEPLLTVADYR
                     KGDPLARQGDEEMEQFFILEGMLKRVVSNPEGREMILRFAAETEMDTSYAAWRLRTPV
                     PYSIVAVTKVRAAELTMPAWAEFLDRNPALKARFEYEVMKLMSEVMAHTITLHLLD"
     gene            503..2410
                     /locus_tag="VGO02_11565"
     CDS             503..2410
                     /locus_tag="VGO02_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478361.1"
                     /translation="MTALVALVSVVASVARTLGSGQDLNFDLITYHFYSGFSAFSDRV
                     GLDYLAAGAAGYQSPLPYALLYFLDGAGVPPIVNAALHACLHALSLLFLFLLTKLLLR
                     GTAAEHSRAAWIAFWLLGAVAPIYWQLVGTSFADALTAALLLAALWLSAEALPAEDRP
                     GSLRMLALGAGLAGLALAARVHNAIFAAALYSALLLVRFPDRRTYFRFAAVFAAACAA
                     AWLVCFGPWAWRMLREFGSPVFPLFNALFRSPDYPGVNLHLVGFVPQNLGDLLLLPLR
                     MATYQEWRFGEKPFADVRPALLVLSAAACLLALIYRQATGKAEANEPSRARRLVLLFF
                     LIGALLWLVTSANNRYGLALLLLAGPVCGVLLQRLVPLRYVLLIVGAAVLWQAWDQQR
                     YFTQYRWASGPWSARYFDWETPSALASEPAVYLGFGYKTASTLAPRVHPGSRHVALVA
                     QYSIGLDHPGARRVRQMIDAPVGKIYGVFDYYYTQQSDPAAKSIKTYFAEHLRLWGLD
                     FAAAACSPVRLKPVAGAFRGGQPPQLILCELRGAAASDQEAMQVKYRQFEKTLAPLAA
                     ACPRILGAAVSIVRVHGQWQVTSFASAEYRLEFDDDGAFSLQLLRPPYSTIPLGKLAG
                     GAIVLRRPGCL"
BASE COUNT          347 a          925 c          868 g          477 t
ORIGIN      
        1 cgtcgagcag gtgcagcgtg atcgtgtgcg ccatcacctc ggacatcagc ttcatcactt
       61 cgtattcgaa gcgcgccttg agcgcggggt tgcggtcgag gaactcggcc cacgccggca
      121 tcgtcaattc ggccgcgcgc accttggtaa cggcgacgat cgagtagggc accggcgtgc
      181 gcagccgcca ggccgcgtac gaggtgtcca tctcggtctc ggcggcgaag cgcaggatca
      241 tctcgcggcc ttccgggttg gacaccacgc gcttgagcat gccctcgagg atgaagaact
      301 gctccatctc ctcgtcgccc tggcgcgcga gcgggtcgcc tttgcggtag tcggcgacgg
      361 tgagcagcgg ctcgagctcg gcccactgcg ccgggctcat gccctgcagc aggaggttct
      421 gccgcagctg caaacggatg ctgttacgct gcggatggac ggccagggag gtcatatatt
      481 gcgttggctg gattctagac ggttgaccgc actggtcgcg ctggtcagcg tcgtcgcctc
      541 cgttgcgcgc acgctcggca gcggccagga cctgaatttc gacctcatca cgtaccactt
      601 ctactcgggc ttcagcgcgt tcagcgaccg cgtgggcctc gactacctgg cggccggcgc
      661 ggcgggctac cagtcgccgc tgccctatgc gctgctgtat ttcctcgacg gcgccggcgt
      721 gccgccgatc gtcaacgccg cgctgcacgc ctgcctgcat gcgctcagcc tcctgttcct
      781 gttcctgctg acgaagctgc tcctcagggg gaccgccgcc gagcacagcc gggcggcgtg
      841 gatcgctttc tggctgctgg gcgcggtggc gccgatctac tggcagctcg tcggcacgtc
      901 gttcgccgac gcgctgacgg ccgcgctgct gctcgccgcg ctctggctca gcgccgaggc
      961 gctgccggcc gaggaccggc cggggtcgct caggatgctg gctctgggcg ccggcctcgc
     1021 ggggctggcg ctcgccgcgc gcgtgcacaa cgcgatcttc gccgcggcgc tgtacagcgc
     1081 gctgctgctg gtgcgcttcc cggatcgcag gacgtatttc cgcttcgccg ccgtgttcgc
     1141 cgctgcgtgc gcggctgcgt ggctcgtatg cttcgggccc tgggcgtggc gcatgctgcg
     1201 cgagttcggc agtccggttt ttccgctgtt caacgcgctg ttccgctcgc ccgactaccc
     1261 cggcgtgaac ctgcacctgg tcggcttcgt gccgcagaac ctcggcgacc tgctgctcct
     1321 gccgctgcgc atggcgacct accaggagtg gcgcttcggg gagaagccgt ttgccgacgt
     1381 gcgcccggcg ctgctcgtgc tgagcgccgc ggcctgcctg cttgccttga tctatcggca
     1441 ggcgaccggg aaagccgagg cgaacgagcc atcccgggca cgcagactcg tgctgctgtt
     1501 cttcctgatc ggcgcgctgc tgtggctcgt gacctcggcc aacaaccgct acggcctggc
     1561 gctcctgctc ctcgccgggc cggtctgcgg cgtgctgctg caacggcttg tgccgctgcg
     1621 ctacgtgctc ctgatcgtcg gcgctgcggt cctctggcag gcgtgggacc agcagaggta
     1681 tttcacccag taccggtggg catcgggccc ctggagcgcg cgctatttcg actgggagac
     1741 gccgagcgcg ctggcgagcg agcccgcggt ctacctcggc ttcggctaca agaccgcctc
     1801 gacgctcgcg ccgcgcgtgc acccgggctc gcgccacgtc gcgctcgtcg cgcagtactc
     1861 gatcggcctc gaccatccgg gcgcgcggcg cgtgcgccag atgatcgacg cgccggtggg
     1921 caagatctac ggcgtcttcg actactacta cacgcagcag agcgatccgg cggcgaagtc
     1981 gatcaagacc tatttcgccg agcaccttcg tctctggggc ctggatttcg ccgctgcggc
     2041 gtgctcgccg gtccgcctca agccggtcgc cggcgccttc cgtggcggcc agccgccgca
     2101 gctcatcctg tgcgagctgc ggggcgcggc ggcaagcgac caggaagcca tgcaggtcaa
     2161 ataccggcag ttcgagaaga ccctcgctcc gctcgccgca gcctgccccc gcatcctcgg
     2221 cgcagcggtt tcgatcgtcc gggtccacgg ccaatggcag gtgacgagct tcgcctcggc
     2281 cgaataccgg ctcgaattcg acgacgacgg cgctttcagt ctgcagcttc tgcgcccgcc
     2341 ctactcgacg atcccgcttg gcaagctggc cggcggcgcg attgtgctgc gccggcccgg
     2401 ctgcctttag gacccttgac ctcagtcaag gcccgcggcg gacgcggatt cttaccatta
     2461 caatccctca caggaggatg gccatgggca cggtcgaacc ccaagtcgct accgatgcgc
     2521 aaacgcaaac agtcgacggt tttcagctcg taatcgatgc cctcaagctg aacggcctcg
     2581 acaccgtcta cggcctgccg ggcattccga tcaccga
//
LOCUS       DASYJA010000537         4273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2168536, whole genome shotgun sequence.
ACCESSION   DASYJA010000537 DASYJA010000000
VERSION     DASYJA010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2168536"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VGO02_11570"
     CDS             <1..477
                     /locus_tag="VGO02_11570"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEV7478362.1"
                     /translation="WRLVDVVQTRFTTEGPRTTRAPQSEWRSAVTPDMLDALIVRPER
                     MSAWALHKYIQHLSGNKQKTELYEIALWKKLFYPLAALVMMALALPFAYMQARAGAVG
                     VKVFLGIILGIFFHMLNSLFSHIALLQNWPSLAAAVMPSIAFLAAAMAMMWWVERR"
     gene            complement(478..870)
                     /locus_tag="VGO02_11575"
     CDS             complement(478..870)
                     /locus_tag="VGO02_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEV7478363.1"
                     /translation="MSVTAPPLPRRLASMGYEAILLFGVGFFAAWLFFFASGGRDATH
                     GWERYVLQLFILAVFAAYFLWCWLRGGQTLAMKAWHVRVVGVTPQKALLRFLLAFVLL
                     PVSIPWALLDRDGQFLHDRLAGTRLVQA"
     gene            complement(867..1358)
                     /locus_tag="VGO02_11580"
     CDS             complement(867..1358)
                     /locus_tag="VGO02_11580"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HEV7478364.1"
                     /translation="MLRVLLAALALCTAAGVAAQQPSADVAPKAAPKPAAKPPAKKKA
                     AAVVRPAWIELSPEQRQTLAPLQEDWENFERERRLKWIGIAKRYPKMKPEEQARVQRR
                     MQAWAKLTPEQRRQARESYKNLAKDKDKHGNLREQWAEYQALPPEERLTLMPAPAEKK
                     QRR"
     gene            complement(1358..1729)
                     /locus_tag="VGO02_11585"
     CDS             complement(1358..1729)
                     /locus_tag="VGO02_11585"
                     /inference="COORDINATES: protein motif:HMM:NF023699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HEV7478365.1"
                     /translation="MHFGNRIRQVLNQGLRLSARQAERLHAARERALTARRPEPVGAL
                     AWADNVLGSFGGWSGLSLRLVAPLAALTVSVAALYTWQQNRVISDLAEIDSMLLTDDL
                     PIDAYLDRGFQNYLKTRATEE"
     gene            complement(1735..2301)
                     /locus_tag="VGO02_11590"
     CDS             complement(1735..2301)
                     /locus_tag="VGO02_11590"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV7478366.1"
                     /translation="MASRSELSAFLASVERRAFKQALFAVRDEEAALDIVQDAMLRLA
                     EKYGNRPAAELPLLFHRIVQNAVRDWFRRQKVRSLWTTLLSSLSGGGEDDDRDPLETL
                     EAADGSNPQDSPAEHLEQAQVLEIIEQELRRLPERQRQAFIMRYWEELDVAETAKVMG
                     CSEGSVKTHCSRATHALAIALKARGITL"
     gene            2381..4108
                     /locus_tag="VGO02_11595"
     CDS             2381..4108
                     /locus_tag="VGO02_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HEV7478367.1"
                     /translation="MDRIKTSTLSGAEIVIRCLQEEAVEYVFGYPGGAVLFIYDELFK
                     QDKVKHVLVRHEQGAVHAADGYSRSSQKVGVALVTSGPGVTNAVTGIATAYMDSIPMV
                     VITGQVPTHAIGLDAFQECDTVGITRPIVKHNMLVKDVKDLATAMKKAFHIATTGRPG
                     PVVVDIPKDVTQQTCEFSYPEEVSLRSYQPVRKGHPGQIKKAVQLLLEAQRPMVYSGG
                     GVVLSNSSSQLRDLVKQTGFPITNTLMGLGAYPATDKQFLGMLGMHGTYEANMAMQHC
                     DVLLAIGARFDDRVIGNPAHFAQNPRKIIHIDIDPSSISKRVKVDVPIVGNIPDVLDE
                     VLKLIRTPGEKRIDEKSIAPWWKQIEEWRAKDCLKYDRNSKIIKPQFVLEKLYEVTKG
                     EAFVTSDVGQHQMWAAQFYKFDQPRRWINSGGLGTMGFGLPAAMGVQMANPGATVACV
                     TGESSIQMCLQELSTCKQYRLPIKIVNLNNKYMGMVRQWQQFFHGNRYSESYMDALPD
                     FVKVTEGYGHRGVLIEKPADVEPALRDAFSRKSELVFLDFLTDQAENVYPMIPGGKGI
                     TEMILSEEL"
     gene            4137..>4273
                     /gene="ilvN"
                     /locus_tag="VGO02_11600"
     CDS             4137..>4273
                     /gene="ilvN"
                     /locus_tag="VGO02_11600"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEV7478368.1"
                     /translation="MSRHIVSVLVENEAGALSRIAGLFSARGYNIESLTVAPTEDASM
                     SR"
BASE COUNT          739 a         1388 c         1411 g          735 t
ORIGIN      
        1 tggcgcctgg tcgacgtggt gcagacgcgc tttaccaccg aagggccgcg caccacccgc
       61 gcgccgcagt cggaatggcg ctcggcggtg acccccgaca tgctcgatgc gctgatcgtg
      121 cggcccgagc gcatgtcggc atgggcgctg cacaagtaca tccagcacct ctccggcaac
      181 aagcagaaga ccgagctcta cgagatcgcg ctgtggaaga agctgttcta tccgctcgcg
      241 gcgctggtaa tgatggcgct cgcgctgccc ttcgcctaca tgcaggcgcg cgccggcgcc
      301 gtcggcgtca aggtgttcct cggcatcatt ctcggtatct tcttccacat gctgaacagc
      361 ctgttctcgc acatcgccct gctgcagaac tggccgtcgc tcgccgccgc ggtgatgccc
      421 tcgatcgcct tcctcgccgc ggcgatggcg atgatgtggt gggtcgagcg gcgctagcta
      481 ggcctggacc aggcgggtgc cggcgaggcg gtcgtgcagg aactggccat cccgatcgag
      541 caaggcccac gggatcgaga ccggaaggag cacgaaggcg agcaggaagc gcagcagggc
      601 cttctgcggc gtgacgccga ccacgcggac gtgccaggcc ttcatcgcca gcgtctggcc
      661 gccgcgcagc cagcaccaca ggaagtaggc ggcgaacacg gcgaggatga agagctgcag
      721 cacgtagcgc tcccagccgt gggtggcgtc gcgcccgccg ctggcgaaga agaacagcca
      781 cgcggcgaag aagccgacgc cgaacagcag gatcgcctcg tagcccatgc tcgccaggcg
      841 ccgcgggagc ggcggcgcgg tcacgctcac cgccgctgtt tcttctcggc cggggccggc
      901 atcagcgtca ggcgctcctc cggcggcagc gcctggtatt cggcccactg ttcgcgcagg
      961 ttgccgtgct tgtccttgtc cttggcgagg ttcttgtagc tttcccgcgc ctggcggcgc
     1021 tgctcgggag tgagcttggc ccaggcttgc atgcgccgct gcacgcgcgc ctgctcttcg
     1081 ggcttcatct tcggatagcg cttggcgatg ccgatccact tgagccgccg ctcgcgctcg
     1141 aagttctccc agtcctcctg cagcggcgcg agggtctgcc gctgctcggg gctcagctcg
     1201 atccatgcgg ggcgcaccac ggcggcggcc tttttcttgg ccggcggctt ggcggcgggc
     1261 ttgggggcgg ctttcggtgc gacgtcggcg gacggctgct gggcagcgac cccggctgcg
     1321 gtgcacagcg ccagggcagc gagcaggacg cgcagcatca ctcctccgtt gcgcgtgttt
     1381 tcaggtagtt ctggaagccg cgatcgaggt aggcgtcgat cggcaggtcg tcggtgagca
     1441 gcatcgagtc gatctccgcc aggtcggaga tcacccggtt ctgctgccat gtatagaggg
     1501 cggcgaccga gaccgtcagt gccgccagcg gggccaccag ccgcagggac aggccgctcc
     1561 agccgccgaa gctgcccagc acgttgtcgg cccaggcgag ggcgccgacc ggctcggggc
     1621 gccgggcggt gagcgcgcgc tcgcgcgcgg cgtgcagccg ctcggcctgc cgtgcgctga
     1681 gacgcagccc ttggttgagg acctgccgga tccgattgcc gaagtgcatt tcgttcataa
     1741 cgtaataccg cgtgccttga gcgcgattgc caaagcatgt gtcgcccggg agcagtgcgt
     1801 tttcacgctg ccctccgagc atcccatgac tttcgcggtc tcggcgacgt ccagttcctc
     1861 ccagtaacgc atgatgaaag cctggcgttg acgttccggc aagcgccgga gctcttgctc
     1921 aataatttca agcacttgag cttgctcgag gtgctccgcc ggggagtcct gcgggttcga
     1981 cccatcggcc gcctccaggg tttccagcgg atcgcggtcg tcatcttcgc cgccgcccga
     2041 gagcgaggac agcagagttg tccacaggga gcggaccttc tggcggcgga accagtcgcg
     2101 cactgcgttc tgcacgatgc ggtggaagag cagcggcagc tcggccgccg gccggttccc
     2161 gtacttctcg gcaagccgca gcatggcgtc ctgcacgatg tcgagcgccg cctcctcgtc
     2221 gcgcaccgcg aacaaggcct gcttgaacgc ccgccgttcg acgctcgcca ggaacgccga
     2281 gagctcgctg cgggatgcca gggtgaaggc cctccgtggt tcgcgttgta gtcgggcaag
     2341 acgatgttac catcatggtt tacaaaggtt tttgcccccc atggaccgca tcaagacgag
     2401 cacgctgagt ggagccgaga tcgtgattcg ctgcctgcag gaagaggccg tcgaatacgt
     2461 attcggctat cccggcggcg cggtcctgtt catctacgac gagctgttca agcaggacaa
     2521 ggtgaagcac gtgctggtgc gccacgagca gggcgcggtg cacgcggccg acggctattc
     2581 gcgctcgtcg cagaaagtcg gcgtcgcgct ggtcacctcg gggccgggcg tcaccaacgc
     2641 ggtgaccggc atcgccaccg cgtatatgga ttcgatcccg atggtggtga tcaccggcca
     2701 ggtgccgacg cacgccatcg gcctggacgc cttccaggag tgcgacaccg tgggcatcac
     2761 gcgccccatc gtgaagcaca acatgctggt gaaggacgtg aaggacctcg ccacggcgat
     2821 gaagaaggcg ttccacatcg cgaccaccgg ccgtcccggc ccggtcgtgg tggacattcc
     2881 caaggacgtc acccagcaga cctgcgaatt ctcgtacccg gaggaggtct cgctgcgctc
     2941 ctaccagccg gtgcgcaagg gccaccccgg gcagatcaag aaagcggtgc agctgctgct
     3001 cgaggcgcag cggccgatgg tctacagcgg cggcggcgtc gtgctttcga attcttccag
     3061 tcagttaaga gatttagtaa agcagacagg atttcccatc accaacacgt tgatggggtt
     3121 gggcgcgtat ccggccaccg acaagcagtt cctcggcatg ctcggcatgc acggcacgta
     3181 cgaggcaaac atggccatgc agcactgcga cgtgctgctc gccatcggcg cgcgcttcga
     3241 cgaccgcgtc atcggcaacc cggcgcactt cgcgcagaac ccgcgcaaga tcatccatat
     3301 cgacatcgac ccgtcgtcga tctcgaagcg ggtgaaggtg gatgtgccga tcgtcggcaa
     3361 catccccgac gtcctggatg aggttttgaa actcataaga acccccgggg aaaagcggat
     3421 tgatgaaaaa tcaattgctc cctggtggaa gcagatcgag gagtggcggg cgaaggactg
     3481 cctcaagtac gaccgcaact cgaagatcat caagccgcag ttcgtgctgg agaagctcta
     3541 cgaagtcacc aagggcgaag cgttcgtcac ttccgacgtc ggccagcacc agatgtgggc
     3601 ggcgcagttc tacaagttcg accagccgcg gcgctggatc aattccggcg gcctgggcac
     3661 catgggcttc ggcctgccgg cggcgatggg cgtgcagatg gccaatccgg gcgcgaccgt
     3721 cgcctgcgtc accggcgaat cgtcgatcca gatgtgcctg caggagctct ccacctgcaa
     3781 gcagtaccgc ctgcccatca agatcgtgaa cctcaacaac aagtacatgg gcatggtgcg
     3841 ccagtggcag cagttcttcc acggcaaccg ctactccgag tcctacatgg acgcgctgcc
     3901 cgacttcgtg aaggtgaccg agggctacgg ccaccgcggc gtgctgatcg agaagccggc
     3961 cgacgtcgaa cctgcgttgc gggacgcctt cagtagaaaa tcagaactgg ttttcctgga
     4021 cttcctcact gaccaggccg agaacgtcta tccgatgatc cccggcggca aggggatcac
     4081 ggaaatgatc ctgtcggaag agctttgagc agcggcctct tcgcggtcca cccgctgtga
     4141 gccggcatat cgtcagcgtc ctcgtggaaa acgaggcggg ggcgttgtcg cgcattgcgg
     4201 gcctcttttc cgcgcgcggc tacaacatcg agtcgctcac cgtggcgccg accgaggacg
     4261 cctcgatgtc gcg
//
LOCUS       DASYJA010000538         2616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_899962, whole genome shotgun sequence.
ACCESSION   DASYJA010000538 DASYJA010000000
VERSION     DASYJA010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899962"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1951)
                     /locus_tag="VGO02_11605"
     CDS             complement(<1..1951)
                     /locus_tag="VGO02_11605"
                     /inference="COORDINATES: protein motif:HMM:NF006196.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HEV7478369.1"
                     /translation="MQSLWNDAEAAQFAGPAFGPLGPRVYTSRLLGRDKSLVLHGGGN
                     TSVKLKEKNLFGEEQELLYVKGSGWDLESIEAAGFTPLPLDYMRRLTTLERLSDPQMV
                     SELATHSLRAGAPAPSVETLLHALLPHKYVDHTHADAVLSVSNSPDGEKRIRDVYGNR
                     VVVIPYIMPGFDLAAYCARAFAKEATKSTVGMVLLSHGVFSFGADAREAYERMLELVS
                     MAERYLQAKKAWSIGLAAANAPAFEREAAARLRHEVSRAAGFPLLCLCNDSPRFLAFA
                     QHPDVATISQSGPATPDHVIRTKPVPMLGRDVDGFSSSYKNYFERNSKKAKEPKTMLD
                     AAPRVALDRTMGFAAFGRTAKDTRIAEELYEHTIDVILRAEALGGWRALDDQHTFDVE
                     YWDLEQAKLRKAGAPPVFTGEVALVTGAASGIGKACAEAFLKRGAAVVGLDLNPAVEK
                     LWKKPEFLGITCNLTDAAAIGASLDQAVKQFGGVDMLVLNAGIFPASAAIKDIVAESW
                     RNAMTVNVEGSLLVMQACHPLLKLAPRGGRVVVIGSKNVPAPGPGAAAYSASKAALNQ
                     LARVAALEWAKDGIRINSLHPDAVYDTGLWTEELLAARAKAYNMTIEQYKKKNLLKTE
                     VSSKDVAELAAEMCGTLFAKTTAAQV"
     gene            1950..2390
                     /locus_tag="VGO02_11610"
     CDS             1950..2390
                     /locus_tag="VGO02_11610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV7478370.1"
                     /translation="MARIILSAVWRIELMTWEKARAQAAPIRLAVFVEEQGVPAELEM
                     DEMDARCVHALAFEDSRAVGTGRLLPDGHIGRMAVLKEFRGKGAGGALLAKLMEAAKS
                     RGDREVVLSAQVHAVPFYQAYGFELFGEVYEEAGIPHRDMRRDL"
     gene            complement(2387..>2616)
                     /locus_tag="VGO02_11615"
     CDS             complement(2387..>2616)
                     /locus_tag="VGO02_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478371.1"
                     /translation="LLDAAEVDRPKERFAAALGPAVEPLFTLLAESKIVNERYGTRCS
                     TVLIEGKNTIRYAERSFSPDGAGQETLHYDF"
BASE COUNT          403 a          852 c          908 g          453 t
ORIGIN      
        1 ggacctgggc cgcggtggtc ttggcgaaca gcgtcccgca catctcggcg gcgagctcgg
       61 cgacgtcctt cgaggagacc tcggtcttga gcaggttctt cttcttgtac tgctcgatcg
      121 tcatgttgta ggccttggcg cgcgcagcga gtagctcctc ggtccacagg ccggtgtcgt
      181 agaccgcgtc gggatgcagc gagttgatgc ggatgccgtc cttcgcccac tccagcgccg
      241 cgacgcgcgc gagctggttg agcgccgcct tggaggccga gtaggccgcc gcgccgggcc
      301 cgggcgccgg cacgttcttc gagccgatca ccaccacgcg gccgccgcgc ggggcgagct
      361 tgagcagcgg atggcaggcc tgcatgacca gcaggctgcc ttcgacgttc accgtcatcg
      421 cgttgcgcca gctctcggcg acgatgtcct tgatcgccgc ggaggcgggg aagatgccgg
      481 cgttgaggac cagcatgtcc acgccgccga actgctttac ggcctgatcg agactggcgc
      541 cgatggcggc agcgtcggtg aggttgcagg tgatgccgag gaattccggc tttttccaca
      601 gcttttccac ggcaggattc aagtccaggc cgaccaccgc ggcgccgcgt ttcaggaacg
      661 cctcggcgca ggccttgccg atgcccgagg ccgcgccggt gaccagcgcc acctctccgg
      721 taaagaccgg cggcgcgccg gccttgcgca gcttggcctg ctcgaggtcc cagtactcga
      781 cgtcgaaggt gtgctggtca tcgagcgccc gccagccgcc gagcgcctcc gcgcgcagga
      841 tgacgtcgat ggtgtgctcg tagagctctt cggcgatgcg cgtgtccttg gccgtgcggc
      901 cgaaggcggc aaaccccatc gtgcggtcga gcgcgacgcg cggcgccgca tcgagcatgg
      961 tcttgggctc ttttgccttc ttcgaatttc tttcgaagta attcttgtag gaagaggaaa
     1021 agccatccac gtcgcgcccg agcatcggca ccggcttggt gcggatgacg tggtcgggcg
     1081 tcgccgggcc gctctgcgag atggtggcga cgtcgggatg ctgcgcaaaa gcgaggaagc
     1141 gcggcgagtc gttgcacagg cacagcagcg gaaagcccgc ggcgcgggat acctcatggc
     1201 gcagcctggc cgctgcctcg cgctcgaagg ccggcgcgtt cgccgccgcc aggccgatgc
     1261 tccaggcctt cttcgcctgc aggtagcgct cggccatcga caccagctcg agcatccgct
     1321 cgtaggcctc gcgcgcatcg gcgccgaacg agaacacgcc gtgcgacagc agcaccatgc
     1381 cgacggtgct cttcgtcgcc tccttcgcga aagcgcgcgc gcagtaagcg gccaggtcga
     1441 agcccggcat gatgtaggga atcaccacca cgcggttgcc gtagacgtcg cggatgcgct
     1501 tctcgccgtc gggcgagttg gagaccgaca ggaccgcgtc ggcgtgcgta tggtcgacgt
     1561 atttgtgcgg cagcagggcg tgcagcagcg tctcgaccga gggcgccggc gcccccgcgc
     1621 gcagcgagtg ggtggcgagc tcgctcacca tctgcggatc ggacagccgc tcgagcgtcg
     1681 tcaggcgccg catgtagtcg agcggcagcg gcgtgaagcc cgccgcctcg atgctctcga
     1741 ggtcccagcc gctgcctttc acgtagagca gctcctgctc ctcgccgaac agattctttt
     1801 ccttcagctt gaccgaggta ttgccgccgc cgtgcaggac cagcgatttg tcgcgcccca
     1861 gcaggcgcga ggtgtagacg cgcgggccca atggtccaaa agcaggccct gcgaactgcg
     1921 ccgcttccgc gtcgttccat agactttgca tggcgcgaat tatactttcc gcggtgtggc
     1981 gcattgagct gatgacatgg gaaaaggcgc gcgcgcaggc cgcgccgatc cgcctcgcgg
     2041 tgttcgtcga ggagcagggc gttccagcgg agctggagat ggacgagatg gatgccaggt
     2101 gcgtccacgc cctcgcgttc gaggattcgc gtgcagtggg caccgggcgg ctgctgcccg
     2161 acggccacat cggccgcatg gcggtgctga aggagttccg cggcaagggc gcaggcggcg
     2221 ccttgctcgc caagctgatg gaagcggcca agtcgcgcgg cgatcgcgaa gtcgtgcttt
     2281 cggcgcaggt gcacgcggtg ccgttctacc aggcgtacgg cttcgagctg ttcggcgagg
     2341 tctacgagga agccggcatc ccgcaccgcg atatgcggcg cgatctctag aagtcgtagt
     2401 gcagggtttc ctggccggcg ccgtcggggg agaaactccg ctccgcgtag cggattgtgt
     2461 tttttccttc tatcaaaacc gtcgagcaac gggttccata ccgctcgttc acgatcttcg
     2521 attcagcgag caacgtgaac agcggctcga cagcaggccc gagcgcggcg gcgaagcgct
     2581 ccttcggccg gtcgacctcg gccgcatcga gcaggg
//
LOCUS       DASYJA010000539         4072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_536062, whole genome shotgun sequence.
ACCESSION   DASYJA010000539 DASYJA010000000
VERSION     DASYJA010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536062"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VGO02_11620"
     CDS             <1..484
                     /locus_tag="VGO02_11620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV7478372.1"
                     /translation="GAFETLYTRHRGRLYRFVLRSVKASGTAEELFQEVWIRVIEARD
                     RYTPQARFTTWLYTIAHNLLTDHWRRKGLTLVELDEDVHAPAPDDPARSAEARQALSR
                     FAAALEALPPLQRQAFLLHEEGGLSIAEIAQATGSNEEAAKSRLRYAMAKLKSAVDDG
                     "
     gene            477..1199
                     /locus_tag="VGO02_11625"
     CDS             477..1199
                     /locus_tag="VGO02_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478373.1"
                     /translation="MADERDPQVSRSYRELGAEEPPRALDEAILAASRRAISDEMRSR
                     PAPLVVPTGRRRWYFPVAAAAIITLAVAVTVQVERQKPDDELIVATAPPEARKEEPAV
                     PEEKKQRAFKAPVEQAKPAPKPFAADPPAELAKSNQEARARSDAARDSVVAAPAPAPA
                     PEPARREMESQRPQASTSAGARATGQLQSKVETPEAWLERIAALRKEGKHEEADKALA
                     EFRKRYPDYRLTDEMRAKVEKK"
     gene            complement(1201..1929)
                     /locus_tag="VGO02_11630"
     CDS             complement(1201..1929)
                     /locus_tag="VGO02_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV7478374.1"
                     /translation="MISLKNVSKWYGGFQVLTDCSTEVAKGEVVVVCGPSGSGKSTLI
                     KCVNALEPFQKGTITVEGISVGDPKTDLPKLRARIGMVFQNFELFPHMSVTQNLTLGQ
                     VKVLGRGQDEARERGLKLLDRVGLLAHKDKFPGQLSGGQQQRVAIARALSMDPIAMLF
                     DEPTSALDPEMINEVLDVMVDLAKEGMTMMVVTHEMGFARKVAHRVIFMDAGKIVEDA
                     KKDDFFGTPRSERAQLFLSKILHH"
     gene            complement(1926..2612)
                     /locus_tag="VGO02_11635"
     CDS             complement(1926..2612)
                     /locus_tag="VGO02_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV7478375.1"
                     /translation="MDFDFDVIQRSWGYLFREGMTFTLTLTALAMVGGIVFGTLLAMM
                     RLASNRIASALAGSYVNLMRSIPLVLVIFWFFFLVPYIGAWIVRSPEPIKVGAFYSAL
                     ITFTMFEAAYYCEIMRAGIQSIPRGQVWSGYALGLNYWQTMGTVVLPQAFRNMLPVLL
                     TQTIILFQDTSLVYVISATDFLGAAAKVANRDYRLVEMYTFVAVVYFIMSYGLSTLVK
                     SLQNRIAIIR"
     gene            complement(2612..3373)
                     /locus_tag="VGO02_11640"
     CDS             complement(2612..3373)
                     /locus_tag="VGO02_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV7478376.1"
                     /translation="MNYNWHWQVLLEAEPGGTGSYLHYLIVGLGWTLVTALAAWVIAL
                     SIGAAVGTLRTTPLKWAVRLGNLYVEIFRNVPLIVQMFLWFFVVPELLPAAIGDWIKQ
                     MPPPWSSYLPAVMCLGIFTSVRVAEQVRAGINSLPRGQRMAGTAMGLTEAQTYRFVIL
                     PQAFRIILPPLTSEFMNIIKNSSVALTIGLLELTGRARAMQEFSFRVFEAFTAATVIY
                     LITNLLVVLLMRALERKVRVPGLIAASGGTLQAGH"
     gene            complement(3405..>4072)
                     /locus_tag="VGO02_11645"
     CDS             complement(3405..>4072)
                     /locus_tag="VGO02_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV7478377.1"
                     /translation="LKVNYQLVTSANRIPLMANGTIDLECGSTTNNIARQEQVGFTIT
                     HFVTANRWIAKKSAKINKLADLKGKTIVSTAGTTNIKQITEINAAQNLGMNIVSANGH
                     PEAFQMVETGRAVAFVMDDILLYSLAAQSRSPKDYAISAEALSVEPYGIMVRKGDTAF
                     KKVVDDAMTQIMKSGQINAIYSKWFLKPVPPKGINLNLPMSAQFKKVIANPTDSGDPA
                     AYK"
BASE COUNT          726 a         1286 c         1403 g          657 t
ORIGIN      
        1 cggggccttc gaaacgctct atacaaggca tcgcggccgg ctttaccgct tcgtgctgcg
       61 cagcgtgaag gctagcggca cggccgagga gctgttccag gaagtctgga ttcgcgtcat
      121 cgaggcgcgc gatcgctaca ctccgcaagc gcgcttcacc acctggctct acaccatcgc
      181 acacaatctt cttaccgacc attggcgccg caagggcctg acgctcgtcg agctcgacga
      241 ggacgtgcac gccccggcgc cggatgaccc ggcgcgctcg gccgaggcgc gccaggcgct
      301 gtcgcgcttt gccgccgcgc tcgaggcgct gccgccgctg cagcgccagg cgttcctgct
      361 gcacgaggaa ggcgggctgt cgatcgctga gatcgcgcag gcgacggggt cgaacgaaga
      421 ggcggcgaag agccgcctgc gctacgcgat ggcgaaactc aagtcggcgg tcgacgatgg
      481 ctgacgagcg cgacccgcaa gtctcgagaa gctatcgcga gctcggcgcg gaggagccgc
      541 cgcgcgccct cgacgaggcg atcctcgccg cctcgcgccg cgccatatcg gatgagatgc
      601 gatctcgccc ggcgcctttg gtcgtgccga ccgggcggcg gcgctggtac ttcccggtcg
      661 ccgccgcggc gatcatcacg ctcgccgtgg cagtgacggt gcaggtcgaa agacaaaagc
      721 ccgacgacga gcttatcgtc gccaccgccc cgcccgaggc gcgcaaggaa gagccggcgg
      781 tgcctgaaga gaagaaacaa cgggccttca aagcgcccgt ggagcaggcc aagccggcgc
      841 cgaagccgtt tgccgcggac ccgccggccg agctggcgaa atcaaaccag gaagcgaggg
      901 cgagaagtga cgcggcgcgc gacagcgtcg tcgccgcccc ggcgccggcg ccggcgcccg
      961 agccggcgcg gcgcgagatg gagtcgcagc gtccgcaggc cagcacatcc gcgggcgcgc
     1021 gcgccacggg gcagctgcag agcaaggtcg agacgcccga ggcgtggctc gagcggatag
     1081 ccgcgctgcg caaagaaggc aagcatgaag aggccgacaa ggcgctcgca gaattccgca
     1141 agcgctatcc cgactatcgc ctcacggacg agatgcgggc gaaggtcgag aaaaagtagc
     1201 ttagtggtgc aggatcttgg agaggaagag ctgcgcgcgc tcggagcgcg gcgtgccgaa
     1261 gaagtcgtcc ttcttcgcgt cctcgacgat cttcccggcg tccatgaaga tgacgcggtg
     1321 cgcgaccttg cgggcgaagc ccatctcgtg ggtcaccacc atcatggtca tgccttcctt
     1381 ggcgaggtcg accatcacgt cgagcacctc gttgatcatc tccgggtcga gcgccgaggt
     1441 cggctcgtcg aacagcatgg cgatcgggtc catcgacagc gcgcgcgcta tcgcgacgcg
     1501 ctgctgctga ccgccggaaa gctgccccgg gaatttgtcc ttgtgcgcga gcagaccgac
     1561 gcggtcgagg agcttgaggc cgcgctcgcg cgcctcgtcc tggccgcggc ccagcacctt
     1621 gacctgaccc agcgtgaggt tctgcgtcac gctcatgtgc gggaacagct cgaagttctg
     1681 gaacaccatg ccgatgcgcg cgcgcagctt gggcaggtcg gtcttggggt cgccgaccga
     1741 tatgccttcg acggtgatgg tccccttctg gaacggctcc agggcgttga cgcacttgat
     1801 gagcgtcgac ttgccgctgc cggaggggcc gcagaccacc actacctcgc ctttggccac
     1861 ctcggtggag cagtcggtga gcacctggaa gccgccgtac cacttgctta cgttcttgag
     1921 cgagatcatc ggatgatggc gatcctgttc tggagacttt tcaccaaagt actcaaacca
     1981 tacgacatga tgaagtagac cacggccacg aaggtataca tctccaccag ccggtagtcg
     2041 cggttggcga ccttggcggc cgcgccgagg aagtcggtcg ccgagatgac gtagaccagc
     2101 gaggtgtcct ggaacaggat gatggtctgc gtcagcagca ccggcagcat gttgcggaag
     2161 gcctgcggca gcaccaccgt gcccatggtc tgccagtagt tgaggccgag cgcatagccc
     2221 gaccacacct ggccgcgcgg gatcgactgg atgccggcgc gcatgatctc gcagtagtac
     2281 gcggcctcga acatggtgaa ggtgatgagc gcggagtaga acgcgccgac cttgatcggc
     2341 tcgggcgagc gcacgatcca ggcgccgatg tagggcacca ggaaaaagaa ccagaagatc
     2401 accagcacca gcgggatcga gcgcatcagg ttgacgtagc tgccggcgag cgccgaggcg
     2461 atgcggttgc tcgcgaggcg catcatggcg agcagcgtgc cgaagacgat gccgccgacc
     2521 atcgccagcg cggtgagcgt cagcgtgaac gtcatcccct cgcggaacag gtagccccag
     2581 gagcgctgga tgacgtcgaa atcgaaatcc atcagtgtcc ggcctgcagc gtcccgccgc
     2641 tggccgcgat cagcccggga acgcgcacct tcctttccag cgcacgcatc agcagcacca
     2701 ccagcaggtt ggtgatcagg tagatcaccg tcgccgcggt gaaggcctcg aagacgcgga
     2761 agctgaattc ctgcatggcg cgggcgcggc cggtcagctc gagcaggccg atggtcagcg
     2821 cgaccgacga gttcttgatg atgttcatga actccgaggt gagcggcggc aggatgatgc
     2881 ggaaggcctg cggcaggatg acgaagcggt aggtctgcgc ctcggtgagg cccatcgcgg
     2941 tgcccgccat gcgctggccg cgcggcagcg agttgatgcc ggcgcgcacc tgctcggcga
     3001 cgcgcaccga ggtgaagata cccaggcaca tcaccgccgg aaggtaggag ctccacggcg
     3061 gcggcatctg cttgatccag tcgccgatcg ccgcgggcag gagctcgggc acgacgaaga
     3121 accagaggaa catctgcacg atcagcggca cgttgcggaa gatctcgacg tacaggttgc
     3181 cgaggcgcac cgcccatttg agcggcgtgg tgcgcagcgt gccgacggcg gcgccgatgg
     3241 acagcgcgat gacccaggcg gcgagggccg tgaccagcgt ccagcccagg ccgacgatca
     3301 ggtaatgcag gtagctgccg gtcccgcccg gctcggcctc gagcaggacc tgccagtgcc
     3361 agttgtagtt cacgtccctc cgagccggaa ctacaggccg atggttactt gtaggcggcc
     3421 gggtcgcccg agtccgtcgg gttggcgatc accttcttga attgcgcgct catcggcagg
     3481 ttcaggttga ttcccttggg cggcacgggc ttgaggaacc acttgctgta gatcgcgttg
     3541 atctgccccg acttcatgat ctgcgtcatc gcgtcgtcga ccaccttctt gaacgcggtg
     3601 tcgcccttgc gcaccatgat gccgtagggc tcgaccgaga gcgcctcggc ggagatggcg
     3661 tagtccttcg ggctgcgcga ctgcgccgcc aggctgtaga gcaggatgtc gtccatcacg
     3721 aaggccaccg cacggccggt ctcgaccatc tggaaggctt ccgggtggcc gttggccgag
     3781 acgatgttca tgccgagatt ctgcgcggcg ttgatctcgg tgatctgctt gatgttggtg
     3841 gtgccggcgg tggagacgat ggtcttgccc ttcaggtcgg cgagcttgtt gatcttcgct
     3901 gacttcttcg cgatccagcg gttggccgtg acgaaatgcg tgatggtgaa gccgacctgc
     3961 tcctgccggg cgatgttgtt ggtggtcgag ccgcactcga ggtcgatggt gccgttcgcc
     4021 atcagcggga tgcggttggc cgaggtgacc agctggtagt tcaccttgag gt
//
LOCUS       DASYJA010000540         4271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_171538, whole genome shotgun sequence.
ACCESSION   DASYJA010000540 DASYJA010000000
VERSION     DASYJA010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171538"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VGO02_11650"
     CDS             <1..344
                     /locus_tag="VGO02_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEV7478378.1"
                     /translation="MNACGHHHVGHIGILGVDKNGEEWYQIEIGGNQGNTHATGPGAA
                     LGKVLGPSFARAEVPDVVQKLLETYLKTRDSEAERFIEVVHRVGIEPFKTAVYGNADQ
                     RAARRRESVAA"
     gene            292..690
                     /locus_tag="VGO02_11655"
     CDS             292..690
                     /locus_tag="VGO02_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HEV7478379.1"
                     /translation="MATLIKERRVVANPSQPEGEVIRLEPQDDPAAIAARLQNVSLIE
                     INFPKFGDGRGFSIARLLRERYGYKGELRAVGQVARDHLYYMEQCGFDAFLLRDGEDV
                     AEALAAFDDFSEAYQSTVAQPVPLFRRRVS"
     gene            690..1397
                     /locus_tag="VGO02_11660"
     CDS             690..1397
                     /locus_tag="VGO02_11660"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEV7478380.1"
                     /translation="MLSTVLRSIAQEFSPAVLASSLSAEDMVLTHAIASAGLPIGIFV
                     LDTGRLNAETLNLISEVRQRYAIDVQVFHPEKKSVDEYVGKHGLNAFYNSVELRKACC
                     GIRKVEPLARALAGKRAWITGLRREQSVGRAELAEREFDAGHGLEKFSPLAAWTEKAV
                     WDYLRVHSVPYNALYDQGYRSIGCEPCSRAVRPGEDVRAGRWWWEQPEHKECGIHVAP
                     VVSKELVFKRQEKIHVA"
     gene            1387..2289
                     /gene="cysD"
                     /locus_tag="VGO02_11665"
     CDS             1387..2289
                     /gene="cysD"
                     /locus_tag="VGO02_11665"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEV7478381.1"
                     /translation="MLPERDHLDWLESEAIYILREVAGQCQNPVLFFSGGKDSICLLR
                     LAEKAFRPGRFPFPLLHIDTGHNYPEVIEFRDRRAAELGERLLVRNLEDSIRRGSVRL
                     TSENQSRNAAQSITLLEAINEFQFDAAIGGARRDEEKARAKERVFSFRDEFGQWDPRN
                     QRPELWSLYNARVNPGEHVRVFPISNWTELDVWQYIAREKLEVPSIYYAHRRPIVRRR
                     GLIVPVTPITPARPNEVVEEVSVRFRTVGDISCTCPVESDASDVVSIIEETTLARLTE
                     RGATRMDDQTSEASMERRKKEGYF"
     gene            2289..3491
                     /locus_tag="VGO02_11670"
     CDS             2289..3491
                     /locus_tag="VGO02_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEV7478382.1"
                     /translation="MQPLRFATAGSVDDGKSTLIGRLLYDARAVQEDQLAAVERASQR
                     RGAAQIDLSLLTDGLEAEREQGITIDVAYRYFATPRRKFIIADGPGHVQYTRNMATGA
                     SNADALVLLLDVKEGLKEQTKKHLRIARLLGIRQVVAFVNKMDAADWSRAAFDAAATD
                     LKAFAAPLGLETLDIIPGSALLGDMVVERGESLGWYRGPTLLERLETLHSGVEAAATG
                     PFRFPIQLVSRPQASVENPAPRGYMGRIESGHLLAGTEVLLLPSGRRTRVRQILSNGA
                     SRELAVAGDSVTLVLADDIDTARGDLIADPMNPPRETRALDVTLVWLGHEPLRAGGRY
                     LVQQGSRRVLAKVQGVAPLALNDIGEARLTLNAPLFVDSYDTVRTTGSLILIDEATNH
                     TVAAGLVK"
     gene            3488..4234
                     /gene="cobA"
                     /locus_tag="VGO02_11675"
     CDS             3488..4234
                     /gene="cobA"
                     /locus_tag="VGO02_11675"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEV7478383.1"
                     /translation="MSGTVHLVGAGPGAPDLLTVRAVRLLGEADIVFHDALVPQAILE
                     LVKGKKVPVGKRSGLRSTNQHFINKQLLDAAKRYQIVVRLKGGDPMLFGRAHEEIAFL
                     IKNQITVKVVPGVTAALAASAELGVSLTRRGEARSVAFVTPRVGEGTRESSWIRAVLA
                     ADTVAIYMGAGEGESIAAALLSAGKAASTPMAIVESASLPDMEVTYGTLQGLAGWRGK
                     GGPVLILLGEVYGEAAAVALRGLRKATRSA"
BASE COUNT          748 a         1437 c         1414 g          672 t
ORIGIN      
        1 gcatgaacgc ctgcggccac caccacgtcg gccacatcgg catcctcggc gtcgacaaga
       61 acggcgagga gtggtaccag atcgagatcg gcggcaacca gggcaacacc cacgccaccg
      121 ggcccggcgc ggcgctgggc aaggtgctcg gcccgtcgtt cgcgcgcgcc gaggtgccag
      181 acgtagtaca gaagcttctc gaaacgtatc tcaaaacccg ggatagcgag gccgaacgct
      241 ttatcgaagt ggtgcaccgc gtcggcatcg aacctttcaa aacggctgtc tatggcaacg
      301 ctgatcaaag agcggcgcgt cgtcgcgaat ccgtcgcagc ctgaaggcga agtcatccgc
      361 ctcgagccgc aggatgatcc tgcggctatc gccgcgcgtc ttcaaaacgt ttccttgata
      421 gaaattaatt tcccgaagtt cggcgacggc cgcggcttct cgatcgcgcg gctcttgcgc
      481 gagcgctacg gctacaaggg cgagctgcgc gcggtgggcc aggtcgcgcg cgaccacctc
      541 tactacatgg agcagtgcgg cttcgatgcc ttcctgctgc gcgacggcga ggatgtcgcc
      601 gaggcgctcg ccgccttcga cgacttcagc gaggcctacc agtccaccgt ggcgcagccg
      661 gtgccgctgt tccgccgcag ggtgagctga tgctcagcac ggtccttcgc tccatcgcgc
      721 aggaattctc gcccgcggtg ctggcgagca gcctgtcggc ggaggacatg gtgctcaccc
      781 acgccatcgc gagcgccggc ctgccgatcg gcatcttcgt gctcgatacc ggacgcctga
      841 acgcggaaac actgaatctg atttctgaag tccgtcagcg ctacgctatc gacgtccagg
      901 tatttcatcc tgagaaaaaa agcgtagacg aatacgtcgg aaagcacggc ctcaacgcgt
      961 tctacaacag cgtcgagctg cgcaaggcct gctgcgggat ccgcaaggtc gagccgcttg
     1021 cccgcgccct cgccggcaag cgcgcgtgga tcaccggcct gcggcgcgag cagtcggtcg
     1081 gccgcgccga gctcgccgag cgcgagttcg acgccggcca cggcctggag aaattcagcc
     1141 cgctcgccgc ctggaccgaa aaggcggtgt gggactacct gcgcgtgcac agcgtgccct
     1201 acaacgccct ctacgaccag ggctaccgct cgatcggctg cgagccctgc agccgcgcgg
     1261 tgcgccccgg cgaggacgtg cgcgccggcc gttggtggtg ggagcagccc gagcacaagg
     1321 aatgcggcat ccacgtcgcg ccggttgttt caaaagaact ggttttcaaa aggcaagaga
     1381 agatccatgt tgcctgagcg cgaccacctc gactggctcg agtccgaggc catctacatc
     1441 ctgcgcgagg tcgcggggca gtgccagaac ccggtgctgt tcttctccgg cggcaaggac
     1501 tcgatctgcc tgctgcgcct cgccgagaag gcgttccgcc cgggcaggtt cccgttcccg
     1561 ctgctgcaca tcgacaccgg ccacaactat cccgaggtga tcgagttccg cgaccggcgc
     1621 gccgccgagc tgggcgagcg gctgctggtg cgcaacctgg aagattcgat tcgaaggggc
     1681 tctgtccgtc tgacttccga gaaccagagc cgcaacgccg cgcagtcgat cacgctgctc
     1741 gaggcgatca acgagttcca gttcgacgcc gccatcggcg gcgcgcgccg cgacgaggag
     1801 aaagcgcgcg ccaaggagcg cgtgttctcc ttccgcgacg agttcggcca gtgggacccg
     1861 cgcaaccagc gccccgagct gtggagcctc tacaacgcgc gcgtcaaccc cggcgagcac
     1921 gtgcgcgtct tcccgatctc caactggacc gagctcgacg tgtggcagta catcgcgcgc
     1981 gagaagctcg aagtgccttc catctactac gcgcaccgcc gcccgatcgt gcgccggcgg
     2041 ggcctgatcg tgccggtgac gccgatcacg cccgcccgtc caaacgaagt cgttgaagaa
     2101 gtctccgtcc gcttccgcac cgtcggcgac atcagctgca cctgccccgt cgaatccgat
     2161 gcgtctgatg ttgtttctat tattgaagag acaacccttg cacggctcac cgagcgaggg
     2221 gcaacgcgca tggacgacca gacttccgag gcttccatgg agcggcgcaa gaaagagggc
     2281 tacttctgat gcagcccctg cgcttcgcca ccgcgggcag cgtcgacgac ggcaagagca
     2341 cgctcatcgg ccggctgctg tacgacgcgc gcgccgtgca ggaagaccag ctggccgccg
     2401 tcgagcgcgc ctcgcagcgc cgcggcgcgg cgcagatcga cctttccctg ctgaccgacg
     2461 gcctggaggc cgagcgcgag cagggcatca ccatcgacgt cgcctaccgc tacttcgcca
     2521 cgccgcggcg caagttcatc atcgccgacg gtcccggcca cgtgcagtac acgcgcaaca
     2581 tggcgacggg cgcgagcaat gcggatgcgc tggttctgct tttggacgtg aaggaaggtc
     2641 tcaaagagca aaccaagaag catctgcgga tcgccaggct gctcggcatc cggcaggtgg
     2701 tcgcgttcgt caacaagatg gacgccgccg attggtcgcg cgccgcgttc gacgcagccg
     2761 cgaccgacct gaaagccttc gctgcgccgc tcgggctcga gacgctcgac atcatcccgg
     2821 gctcggcact gctcggcgac atggtggtcg agcggggcga gtcgttgggc tggtaccgcg
     2881 ggccgacgct gctcgagcgc ctcgagacgc tgcattccgg cgtcgaggcc gccgcgaccg
     2941 ggccgttccg cttcccgatc cagctcgtgt ctcgtcccca ggcaagcgtg gaaaatccgg
     3001 cacctcgcgg ctacatgggg aggatcgagt ccggtcatct cctcgccggc accgaagtgc
     3061 tgctgctgcc ctcgggccgg cgcacgcgcg tgcggcagat cctgagcaac ggcgcgagcc
     3121 gcgagctggc cgttgccggc gactcggtca ccctggtgct cgccgacgac atcgacaccg
     3181 cgcgcggcga cctgatcgcc gacccgatga acccgccgcg cgagacgcgc gccctcgacg
     3241 tcacgctggt gtggctcggc cacgagccgc tgcgcgcagg cgggcgctac ctggtgcagc
     3301 agggctctcg ccgcgtcctc gcgaaggtgc agggtgtggc accgctcgcg ctcaacgata
     3361 tcggcgaggc gcgcctcacg ctgaacgcgc cgctgttcgt cgattcctac gacacggtgc
     3421 gcaccaccgg gtcgctgatc ctcatcgatg aagccacgaa ccatacggtc gccgcggggc
     3481 tggtgaaatg agtggcactg tccatctcgt cggcgccggg ccgggtgcac ctgacctcct
     3541 cacggtgcgc gcggtccggc tgctcggcga ggcggatatc gtttttcatg acgctcttgt
     3601 cccgcaagcg attctggaat tggtaaaggg aaagaaagtc ccagtaggca agcgcagcgg
     3661 cctgcggtct accaatcagc acttcatcaa caagcagctc ctcgacgccg cgaagcgcta
     3721 ccagatcgtc gtgcggctga agggcggcga cccgatgctg ttcggcaggg cgcatgaaga
     3781 aattgctttt ttgataaaaa accaaataac tgtgaaagtc gtgcccggag tcaccgccgc
     3841 gctcgcggcg agcgcggaac tcggcgtctc tctgaccagg cgaggcgagg cgcgcagcgt
     3901 cgccttcgtc acgccgcggg taggcgaggg cacgcgcgag agcagctgga tccgggctgt
     3961 gcttgccgcc gataccgtgg cgatctatat gggcgcgggc gagggcgagt ccatcgccgc
     4021 tgctctgctg agcgcgggaa aggcggcgtc gacgccgatg gcgatcgtgg agagcgcttc
     4081 gttgcccgac atggaagtta cctacggcac cctgcaaggg ctcgctgggt ggagaggaaa
     4141 gggcggcccg gtgctgatcc tgctggggga ggtctacggc gaggcggcgg cggtcgcgct
     4201 gcgcggcctg cgcaaggcga ccaggagcgc gtaggcgccg agcagcacga accacagcag
     4261 cgaccagccg g
//
LOCUS       DASYJA010000541         2409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1261599, whole genome shotgun sequence.
ACCESSION   DASYJA010000541 DASYJA010000000
VERSION     DASYJA010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261599"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VGO02_11680"
     CDS             <1..514
                     /locus_tag="VGO02_11680"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEV7478384.1"
                     /translation="QETAMKFKTLIAVAVAGAFTVPFAALAQGTGGTTTAPSATHPQG
                     NPPAAAPAGSQTSGSGATGEGNASWTKLDKNKDGYLSREELKGDAALSSRFSEMDKDN
                     DGRISKEEFNAASSGAGATGSSTSGKSGSGAGNMGSGSSSEPSSTMGTPKSPSSGGAS
                     SGSSTSKEPK"
     gene            576..923
                     /locus_tag="VGO02_11685"
     CDS             576..923
                     /locus_tag="VGO02_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478385.1"
                     /translation="MKKLFIAAVLAGALPAASAAPRAESALECGIAADMAVVAHSLAH
                     EQVQRAKADAIMVRIYDVAASARGMELMNDILETAYLKKDDAAPAQKFAEELFATCLK
                     AGGNMDTILGKRL"
     gene            complement(920..1870)
                     /locus_tag="VGO02_11690"
     CDS             complement(920..1870)
                     /locus_tag="VGO02_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7478386.1"
                     /translation="MRFLFLLLFCTSVFSQTYPSRPIRLVVGFAPGGAADFVARTLAE
                     PLGRSLGQPIVIDNRPGAGSSIAAEHVAKSAPDGYTLLIASPSSILVNPLINKQSSFQ
                     PMKDLVPVTKVSSSPLVVAVNPAIGVSTLKELIAYAKKNPGKLNYATSGNGSAPHLAA
                     LLFQHVAGVEMVHVPYKGGGPAVQSLLAGDTQISFATPPSVLPLVQAGRLRALAITSR
                     EPTSLMPGVPGMAQAGLPDYEISFWYGFFVPAGTPPAAMQRLFDETARAVKLPEVGRA
                     FATQATEPSASASAADFAAFLAEDAKLWTRLVKDSGAKFD"
     gene            complement(1867..>2409)
                     /locus_tag="VGO02_11695"
     CDS             complement(1867..>2409)
                     /locus_tag="VGO02_11695"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEV7478387.1"
                     /translation="KLLGNTLVAARGPKPTGALRQRGVRIDYSAADPFTTHEMLDELS
                     GLRMQGERVIVQRYGALNRELDAALTARGAEVIEIPTYRWSLPQDTAPLEDLVGRLER
                     GEMHAVVFTNAEQARNLFAVAQRLGKVEFLKKNLNQILVASIGPIASAALREAGVKVG
                     LEASPPKLGALLAALDKALS"
BASE COUNT          435 a          802 c          812 g          360 t
ORIGIN      
        1 acaggagact gcaatgaaat tcaagacttt gattgccgtc gcggtggccg gggcctttac
       61 cgttccgttc gctgcgctcg cgcagggcac cggcggcacg actacagcgc cgagcgccac
      121 ccatccgcaa ggcaacccgc cggccgcggc gcccgccggc tcgcagacct cgggcagcgg
      181 cgcgaccggc gagggcaatg cgagctggac caagctcgac aagaacaagg acggctacct
      241 gtcgcgcgaa gagctgaagg gcgacgccgc cctgtccagc cgcttcagcg agatggacaa
      301 ggacaacgac ggccgcatct cgaaggaaga gttcaacgcc gcctcgagcg gcgcgggcgc
      361 gaccggctcc agcacgagcg gcaagagcgg ctcgggcgcg ggcaacatgg gctccggctc
      421 gagctccgaa ccgagctcga cgatgggcac cccgaagagc ccgagcagcg gcggcgcttc
      481 gagcggctcc tcgaccagca aagaaccgaa gtagcggcaa cgcaccgcat caggggcgtc
      541 cgggggaccg gacgcccatt tttttggaga tcaacgtgaa gaagctgttc attgcggcgg
      601 tgctggcggg cgcgctcccg gcagcgagcg ccgcgccgcg cgccgagagc gcgctcgagt
      661 gcggcatcgc cgccgacatg gccgtggtcg cgcattcgct ggcgcacgag caggtgcagc
      721 gcgcgaaagc cgacgccatc atggtgcgca tctacgacgt ggccgcctcg gcgcgcggca
      781 tggagctgat gaacgatatc ctcgagacgg cctacctgaa gaaagacgac gccgcgcccg
      841 cgcagaagtt cgccgaggag ctgttcgcca cctgcctcaa ggccggcggc aacatggata
      901 cgatcctcgg caagcggctc tagtcgaatt tcgcacccga gtccttcacc agccgcgtcc
      961 agagcttcgc gtcttcggcg aggaacgcag cgaagtcggc ggctgatgcc gaggccgaag
     1021 gctcggttgc ttgggtggca aatgcacgcc cgacttccgg cagcttgacc gctcgcgccg
     1081 tctcgtcgaa aaggcgttgc atcgccgccg gcggtgtgcc tgcaggcacg aagaagccgt
     1141 accagaacga gatttcgtag tcgggcagcc cggcttgcgc catgccgggc acgccgggca
     1201 tcagcgatgt cggttcgcgg ctagtgatcg ccagcgcccg caatcgcccc gcctgcacga
     1261 gcggcaggac ggaagggggc gtggcgaacg agatctgcgt gtcgccggca agcagggact
     1321 gcaccgcagg accgccaccc ttgtagggaa cgtggaccat ctccacgccg gcgacatgct
     1381 gaaaaagcaa tgcggcgagg tgcggcgccg atccgttgcc cgaggtcgcg tagttcagct
     1441 tgcccgggtt ctttttcgcg taagcgatca gctccttcag ggtcgacaca ccaatggcgg
     1501 gattcacagc cactacgagc ggcgaggagc tcacctttgt caccggcacc aggtccttca
     1561 tcggctggaa gctgctctgc ttgttgatga gcgggttcac caggatgctc gacgggctcg
     1621 cgatcaggag ggtatagccg tcgggcgcac tcttcgccac gtgctcggcg gcgatgctcg
     1681 agcccgcgcc ggggcggttg tcgatgacga tcggctggcc gagcgagcgg ccgagcggct
     1741 cggccagcgt gcgcgccacg aaatccgccg cgccgcccgg cgcgaagccg accaccaggc
     1801 ggatggggcg ggacggatag gtctgcgaaa aaaccgacgt gcagaacaac aacaagaaca
     1861 agaatctcac gacaacgcct tgtccagcgc agcgagcagc gcgcccagct tcggcgggct
     1921 cgcctccagc cccactttca cgcccgcctc gcgcagcgcc gccgaggcta tcgggccgat
     1981 ggacgcgacc agaatttgat tcagattttt tttcaggaat tccactttgc ccagtctttg
     2041 agcgacggca aacagattcc tcgcctgctc cgcgttggtg aacaccacgg cgtgcatctc
     2101 gccgcgctcg agcctgccga ccaggtcctc gagcggcgcg gtgtcctgcg gcagcgacca
     2161 gcggtaggtg ggaatctcga tcacttcggc gccgcgcgcg gtgagcgccg catccagctc
     2221 gcggttgagg gcgccgtagc gctgcacgat cacgcgctcg ccctgcatgc gcaggccgct
     2281 cagctcgtcg agcatctcgt gcgtggtgaa cgggtcggcg gcgctgtagt cgatgcgcac
     2341 gccgcgctgg cgcaacgccc ctgttggttt agggcctctc gccgctacca gagtattgcc
     2401 aagcaattt
//
LOCUS       DASYJA010000542         3390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1171134, whole genome shotgun sequence.
ACCESSION   DASYJA010000542 DASYJA010000000
VERSION     DASYJA010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171134"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /gene="rsmD"
                     /locus_tag="VGO02_11700"
     CDS             complement(<1..380)
                     /gene="rsmD"
                     /locus_tag="VGO02_11700"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEV7478388.1"
                     /translation="MHSSKGAQRKNRVRIIGGAHRGRRIAVPDRPGLRPTPDRVRETL
                     FNWLGQRLDGLACLDLFAGSGALGFEAASRGAARVVMVENDRAAFESLKKSLAVIGAR
                     QVELVFADAFDFLRASQDGFDIVFL"
     gene            387..1292
                     /gene="ftsY"
                     /locus_tag="VGO02_11705"
     CDS             387..1292
                     /gene="ftsY"
                     /locus_tag="VGO02_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEV7478389.1"
                     /translation="MVAALSWAARLKAGLARTRDVLNTPVSELFTRRKVDEALFEDLE
                     SALLQADCGVAATQALIGALRKQKLEDGAALKRALKDALADLLLPLEKKLDVSTAKPF
                     VIMVAGVNGSGKTTSIGKLAKWLQAQGRSLLLAAGDTFRAAAREQLVVWGERNGVAVV
                     AQQSGDPGAVTFDAIAAARARGVDVVIADTAGRLPTQLHLMEEIRKVKRVAAKAQAGA
                     PHEVLLVLDANTGQNALAQVKAFDDALGLTGLIITKLDGTAKGGAICAIAKERPVPVL
                     FVGVGESIDDLRPFSAREFAEALIG"
     gene            1285..1938
                     /gene="ftsE"
                     /locus_tag="VGO02_11710"
     CDS             1285..1938
                     /gene="ftsE"
                     /locus_tag="VGO02_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEV7478390.1"
                     /translation="MVSFSAVAKRYPGGQEALRDVSFSLEAGELAFLIGRSGAGKSTL
                     LKLIPAIERPTSGSVIVNGQNVSALKRGAIPYLRRNIGLVFQDQKLLYDRSVYENVML
                     PLAFSAYTPREAGRRARAALDKVGLAGRENANPIQLSGGEQQRVAIARAVVNRPLVLI
                     ADEPTANLDAEAAARVLDIFVAFRQVGVTVLIATHDLALVERYAGRILKLEQGRISA"
     gene            1935..2819
                     /gene="ftsX"
                     /locus_tag="VGO02_11715"
     CDS             1935..2819
                     /gene="ftsX"
                     /locus_tag="VGO02_11715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEV7478391.1"
                     /translation="MRAWLRQHRQAFRLALRRFASGGALSALVIGIALALPAGGYALL
                     ESLSGLATRASFDPQISVFLKNDVKRAEAQALERPLRADRRVAKLRFVPREEALKELN
                     AVQGLPEVTAALGRNPLPDAYVVTARDGNLDSLVADLATLPGVAHVQADSAWARRLAA
                     LAAVGRLGLWLLACLLGLGLVAVTFNTIRLQILTQREEIEVSKLIGATESFIRRPFYY
                     LGLLQGMAGGVIAIAIVWVALALLNREVRVLAESFGSAFRLSYLPAEDALAIIAFAGL
                     LGWLGAHLSVSKHLREID"
     gene            2912..>3390
                     /locus_tag="VGO02_11720"
     CDS             2912..>3390
                     /locus_tag="VGO02_11720"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HEV7478392.1"
                     /translation="MQATLPLSGLFPVPMPTGSLEAYISTVNRFPLLSAEEERSLAQR
                     LRDHDDLDAARQLVLSHLRLVVAVARGYLGYGLPHADLIQEGNIGLMKAVKRFDPERG
                     VRLVSFAIHWIKAEIHEHILRNWRLVKVATTKAQRKLFFNLRSMKRGLAPLTQGDVRR
                     "
BASE COUNT          537 a         1177 c         1130 g          546 t
ORIGIN      
        1 aggaaaacaa tgtcgaagcc gtcctggctc gctctcagaa agtcgaaggc atccgcgaac
       61 acgagctcga cctgccgcgc gccgatgacg gcgagagatt ttttcaggga ttcgaacgcc
      121 gcgcggtcgt tttccaccat gacgacgcgc gccgcgccgc gcgaggcggc ctcgaagccg
      181 agcgcgccgc tgccggcgaa caggtcgagg caggcgaggc catcaagccg ctgcccgagc
      241 cagttgaaga gcgtctcgcg cacgcggtcg ggggtcggcc gcaagcccgg gcgatcgggc
      301 accgcgatgc gccggccgcg atgcgcgccg ccgatgatgc gcacgcggtt tttgcgctgc
      361 gcgcctttgc tagaatgcat tcatccatgg tagcagcgct ttcctgggcg gcgcgcctca
      421 aggcaggcct cgcgcgcacg cgggacgttc tcaacacacc ggtttccgag ctcttcacgc
      481 ggcgcaaggt cgacgaggcg ctgttcgagg atcttgaaag cgcgctgctg caggccgact
      541 gcggcgtggc ggcgacgcag gcgctgatcg gcgcgctgcg caagcagaag ctcgaggacg
      601 gcgccgcgct caagcgcgcg ctgaaagacg cgctggccga tctgctcctg cctttagaga
      661 agaaactgga tgtgagtacc gcaaagccat tcgtcatcat ggtggcgggg gtgaacggct
      721 cgggcaagac cacctcgatc ggcaagctgg cgaagtggct gcaggcgcag ggccgcagcc
      781 tgctgctcgc cgccggcgac acgttccgcg ccgccgcgcg cgagcagctc gtcgtctggg
      841 gcgagcgcaa cggcgtcgcg gtcgtcgccc agcagtccgg cgatccgggc gcggtgacct
      901 tcgacgccat cgctgcggcg cgggcgcgag gtgtggatgt ggtcatcgcc gacaccgccg
      961 gccgcttgcc gacccagctg cacctcatgg aggaaatccg caaagtgaag cgcgtcgcgg
     1021 ccaaagcgca ggcgggcgcg ccgcacgaag tgctgctcgt gctcgacgcc aacaccgggc
     1081 agaacgcgct cgctcaggtg aaggccttcg acgacgccct gggcctcacc ggcttgatca
     1141 ttaccaagct ggacggcacg gccaagggcg gtgcgatctg cgccatcgcg aaggagcgcc
     1201 cggtcccggt gctgttcgtc ggcgtcggcg aaagcatcga cgacctgcgc cctttctcgg
     1261 cgcgcgaatt cgccgaggcg ctgattggtt agcttctccg ccgtcgccaa gcgctaccca
     1321 gggggccagg aggcgctcag ggacgtcagc ttttccctgg aggcgggcga gctcgccttc
     1381 ctcatcggcc gctccggcgc cggcaagtcg acgctgctca agctgatccc ggcgatcgag
     1441 cgcccgacct cgggctcggt gatcgtcaac ggccagaacg tgagcgcgct caagcgcggc
     1501 gcgatcccgt acctcaggcg caacatcggg ctggtcttcc aggaccagaa gctgctgtac
     1561 gaccgcagcg tctacgaaaa cgtgatgctg ccgctcgcct tctcggcgta cacgccccgc
     1621 gaagcggggc gtcgcgcacg ggccgcgctg gacaaggtgg ggctcgccgg gcgcgagaac
     1681 gccaacccga tccagctctc cggcggcgag cagcagcgcg tcgcgatcgc ccgcgcggtg
     1741 gtgaaccggc cgctggtgct gatcgcggac gagccgacgg ccaacctcga cgccgaagct
     1801 gcggcgcgcg tgctcgacat cttcgtcgcc ttccgccagg tcggcgtgac ggtgctgatc
     1861 gccactcacg atctcgccct cgtcgagcgc tacgccggca ggatcctcaa gctcgagcag
     1921 ggcaggatct cggcatgaga gcctggcttc gccagcaccg gcaggccttt cgcctggcgc
     1981 tgcggcgctt cgccagcggc ggcgcgctca gcgcgctagt catcggaatc gcgcttgccc
     2041 tgcccgccgg cggctacgcg ctgctcgaaa gcctgagcgg cctggcgacg cgcgcctcat
     2101 tcgatccgca gatctcggta ttcctcaaga acgacgtgaa gcgcgccgag gcccaggcgc
     2161 tcgagaggcc gctgcgcgcc gaccggcggg tcgcgaagct tcgctttgtc ccgcgcgagg
     2221 aggcgctcaa ggagttgaac gccgtccagg gactgccgga ggttaccgcc gcgctcggcc
     2281 gcaacccgct gccggacgcc tatgtagtga ccgcgcgtga tggcaatctc gactccctgg
     2341 tggccgacct ggcaacgctg cccggcgtcg cgcacgtgca ggccgactcg gcctgggcgc
     2401 ggcgcctcgc cgcgctcgcc gccgtcggcc ggctcggcct gtggctgctc gcctgcctgc
     2461 tcgggctcgg cctggtggcg gtcaccttca acacgatccg gctgcagatc ctcacgcagc
     2521 gcgaggagat cgaggtttcg aagctgatcg gcgccaccga gagctttatc cggcggccct
     2581 tctactacct cggccttctc cagggcatgg ccggcggcgt catcgccatc gccatcgtct
     2641 gggtcgctct agccctgctc aaccgggagg tgcgggtgct cgcagagagc ttcgggtccg
     2701 ccttccggct ttcctatttg ccggcggagg atgccttggc aatcatcgct tttgcggggc
     2761 tcttgggttg gctcggcgcg catttgtctg taagcaaaca cttacgcgaa atagactaat
     2821 ttgctgggct tctccaggga actaacccca cctcttagca ctccaattac ccgagtgcta
     2881 aacttctagc acctagagag gaatgccaca tatgcaagcg actctgcctc tttccggctt
     2941 gttccccgtg ccgatgccca cgggcagcct cgaggcctat atcagcaccg tcaaccgctt
     3001 cccgctgctc agcgcggaag aagagcgttc gctcgcccag cgcctgcgcg atcacgacga
     3061 tctcgacgcc gcacggcagc tggtgctttc gcatctgcgc ctggtggtcg cggtcgcccg
     3121 cggctacctc ggctacggcc tgccgcacgc cgacctgatc caggaaggca acatcggcct
     3181 gatgaaggcc gtcaagcgct tcgacccgga gcgcggcgtg cgcctggtct cgttcgccat
     3241 ccactggatc aaagccgaga tccacgagca catcctgcgc aactggcggc tggtgaaggt
     3301 cgccaccacc aaggcgcagc gcaagctgtt cttcaacctg cgctcgatga agcgcggcct
     3361 cgcgccgctg acacagggcg atgtcagacg
//
LOCUS       DASYJA010000543         1675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2532686, whole genome shotgun sequence.
ACCESSION   DASYJA010000543 DASYJA010000000
VERSION     DASYJA010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2532686"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /gene="pntA"
                     /locus_tag="VGO02_11725"
     CDS             complement(<1..598)
                     /gene="pntA"
                     /locus_tag="VGO02_11725"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012371936.1"
                     /note="forms a tetramer composed of 2 alpha subunits and 2
                     beta subunits in the inner membrane; involved in
                     catalyzing transfer of hydride ion equivalents between NAD
                     and NADP; stereospecific (AB-specific); functions as a
                     proton pump by translocating protons from cytoplasm to
                     periplasm; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HEV7478393.1"
                     /translation="MAQVIGVPRETFAGEKRVATVPEVVEKLIKLGFAVKVESGAGDG
                     ANFGDDTYRAAGAEVVVGAAQLWARSDIVFKVRGPSAEEIGLMHEGQTLIGFIWPAQN
                     PELMQSLAARKVTVLAIDSLPRMLSRAQKMDALTSMAGVTGYRAVIEAAHNFGRFLTG
                     QITAAGKIPPAKVFIAGAGVAGLAAIGTAVGLGAIVRAN"
     gene            complement(645..>1675)
                     /locus_tag="VGO02_11730"
     CDS             complement(645..>1675)
                     /locus_tag="VGO02_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV7478394.1"
                     /translation="SHEFLGRGDGWPGLQAAYGLPQAPRGLDHGLAYEALAAGEIDVM
                     DLYTTDAKIGRYGMAVLEDDKRFFPPYDAVLMYRREALERFAQAFRAFEKLRIDEAAM
                     VRLNARAELDKLAFADVAREFLGSKVQTRKGFWGALFAPDFSRLLVEHVALVLGSLVA
                     ALAVGVPLGVAAAKIRGVAQPVLLATGLVQTIPALALLAFLIPLTGTIGVWPALIALF
                     LYALLPITRNTHAGLVQVPRGLRQAGIALGLRPGEVMRAIELPLALPTILAGVKTSAV
                     INVGTATIAAFIGAGGFGERISQGLALNDHTALLAGALPAAALALAVHVLFELAERSL
                     IRIPLSRR"
BASE COUNT          276 a          577 c          586 g          236 t
ORIGIN      
        1 cgttggcgcg cacgatggca cccaggccga ccgccgtgcc gatcgccgcc aggccggcga
       61 cgccggcgcc ggcgatgaaa accttggccg gaggaatctt gccggcggcg gtgatctgcc
      121 ccgtgaggaa gcggccgaag ttatgcgccg cctcgatcac ggcgcgatag ccggtgacac
      181 cggccatgga agtcagcgcg tccatcttct gggcgcggct cagcatgcgc ggcagcgagt
      241 cgatggccag cacggtgacc ttgcgtgccg cgaggctctg catcagttcg gggttctgcg
      301 cgggccagat gaagccgatc aaggtctggc cctcgtgcat caggccgatc tcttcggcgc
      361 tcgggccgcg caccttgaag acgatgtccg acctggccca gagctgcgcg gcgcccacca
      421 cgacctcggc gcccgcggcg cggtaggtgt cgtcgccgaa gttggcgcca tcgccggcgc
      481 cggactcgac cttcacggcg aagccgagct tgatcagctt ttcgaccacc tcgggaaccg
      541 tggcaacgcg tttctctccc gcgaaggtct cgcgcgggac gcctatgacc tgagccatga
      601 cgcgaatgat acgacagggt cgtgatgcgc ctgtttgaaa atccttatct gcgcgatagc
      661 gggatgcgaa tcagcgagcg ctcggcgagc tcgaacagga cgtgcaccgc gagcgcgagc
      721 gccgccgccg gcagcgcgcc ggcgagcagc gccgtgtggt cgttgagcgc gaggccctgc
      781 gagatgcgct cgccgaagcc gccggcgccg atgaacgcgg cgatggtcgc ggtgccgacg
      841 ttgatcaccg ccgaggtctt cacgcccgcg aggatggtcg gcagcgccag cggcagctct
      901 atggctcgca tgacttctcc gggccgcaga cccagtgcga tgccggcctg tcgcaaacca
      961 cggggcacct gcacgaggcc ggcatgcgta ttgcgcgtga tcggcagcag cgcatagagg
     1021 aacagcgcga tcagcgccgg ccagacgccg atcgtcccgg tgagcgggat caggaaggcg
     1081 agcagcgcga gcgcggggat cgtctgcacc agcccggtgg cgagcagcac cggctgcgcc
     1141 acgccgcgga tcttggccgc ggcgacgccc agaggcacgc cgaccgccag cgccgcaacc
     1201 agcgagccga gcaccagcgc cacgtgctcg accagcagcc gggaaaaatc cggcgcgaac
     1261 agcgcccccc agaagccctt cctggtctgc actttggagc cgaggaactc gcgcgcgacg
     1321 tcggcgaagg cgagcttgtc gagctcggcg cgcgcgttca gccgcaccat ggctgcttcg
     1381 tcgatcctga gtttctcgaa agcgcggaaa gcctgcgcaa accgctcgag cgcctcgcgc
     1441 cggtacatca gcacggcgtc gtagggtggg aagaagcgct tgtcgtcctc gagcacggcc
     1501 atgccgtagc gtccgatctt cgcatcggtc gtgtacagat ccatcacgtc gatttcgccg
     1561 gcggcgagcg cctcgtaggc caggccgtgg tccaggccgc gcggcgcctg cggcaggccg
     1621 taggcggcct gcaacccggg ccagccgtcg ccgcggccga ggaactcgtg cgaca
//
LOCUS       DASYJA010000544         3934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2624511, whole genome shotgun sequence.
ACCESSION   DASYJA010000544 DASYJA010000000
VERSION     DASYJA010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2624511"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="VGO02_11735"
     CDS             <1..1020
                     /locus_tag="VGO02_11735"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7478395.1"
                     /translation="IRCSSSVKRYHRPEEEQDMPASLRWLCAILSAALSWAATAQTYP
                     SQPIRMIAPFPPGGSVDITARLIAEPLAAQLGQRIIVDNRSGASGNIGMEAAARAAPD
                     GYTLVLNTIPLVTNQAMFPNLSWDPIRDFAAIGMVATSPHVVVVPGKSPYHSIDELVR
                     AARASPGRLTYASAGVATTFHLCAELFKDTTHTFILHVPYRGGGPALLDTLAAQVDMS
                     FPTLAAAVPHVKSGALRALAVTDVRRSALLPDVPTLREAGLKDFQFSQWQALLAPAKT
                     PPAIVARLNAALREALKSKDLVEKFQAQAFDPFITSPEEAGEYIAEEAKRLSRVIKTK
                     GITAD"
     gene            1023..1799
                     /locus_tag="VGO02_11740"
     CDS             1023..1799
                     /locus_tag="VGO02_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7478396.1"
                     /translation="MELGLKDKRVLITGGSKGIGLACARAFIAEGARVAIVSRSQANL
                     DAAKKELKNCYCIAGDLIDAAAAARVVEKTESDFGPIDVLVNSAGAAKRTNADDLTPQ
                     AWRAAMDAKYFTYINVIDPLVKRMAERGGGVIVNIIGSGGKLASPIHLPGGAANAALM
                     LATAGLANAYAAKGVRVVGINPGLTNTSRVAEGLKADAKREGISEDEALARAVRKLPL
                     GRLAEPGEIADIAVFAASARGRFLTGANISADGVSTPTVV"
     gene            1839..2366
                     /locus_tag="VGO02_11745"
     CDS             1839..2366
                     /locus_tag="VGO02_11745"
                     /inference="COORDINATES: protein motif:HMM:NF019128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEV7478397.1"
                     /translation="MPSARFRLRLFWYRLDKEACAVALVFGLIVGGFAYAVQAVVGER
                     EAALARLHANHAQNIACLARNVYFEARGEPEAGQYAVAEVTMNRKASGRYPNTVCGVV
                     YERQAFSWTAVDILPEPEGEAWSRAQEIAEEVYYGRHAPVLNGALFFHATYIKPDWAT
                     EKRRVARIGSHIFYR"
     gene            complement(2423..3499)
                     /locus_tag="VGO02_11750"
     CDS             complement(2423..3499)
                     /locus_tag="VGO02_11750"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEV7478398.1"
                     /translation="MKRKLLAAAVTAACASPALAQVPAEIQVYGRVNVSVEHITVSNS
                     SNTTALPNQSTNELVDNSSRIGFRGNKELNTGLKVIWQVESRVKLDDGSGSTFSSRDS
                     YAGLQGGFGTIRAGRSIGPVYYATYDYISLHNHDTGTSSDNLLNPTIFGNAGFMNNLL
                     WYTTPNFSGFSADVAYSFGPTTANEGRAAGVTDQPNYIGLVGAYDKGGLHVAVSYAET
                     KNTSALAVGTGNDDKAYTIGALYDFKAFVLGGLFEKSKSDLAGGGSGDRTYWRVSGML
                     PVGQHEFHVNYGMANDTDPGTDNGAKQYTLAYNYNITKATKVYAFYTTVDNDTNGTYG
                     FRQVTPAVAGADFKSIAIGLRHNF"
     gene            complement(3739..>3934)
                     /locus_tag="VGO02_11755"
     CDS             complement(3739..>3934)
                     /locus_tag="VGO02_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018009096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV7478399.1"
                     /translation="GTSPYRYSLMRRLEFVRARLNAGRPTADLALEAGFADQAHFARL
                     FKGAFGVTPGRYARLAQNGG"
BASE COUNT          646 a         1301 c         1297 g          690 t
ORIGIN      
        1 atccggtgct cctcctcggt gaaacgttat cataggcccg aggaggagca agacatgccg
       61 gcgagcctgc gatggttgtg cgcgatcctg tctgcggcgc tgagctgggc cgcgacggcc
      121 cagacctatc cgagccagcc gatccgcatg atcgcgccct tcccgcccgg cggcagcgtc
      181 gacatcacgg cgcggctgat cgccgagccg ctcgccgcgc agctcggcca gcgcatcatc
      241 gtcgacaacc gcagcggcgc ctccggcaac atcggcatgg aggccgcggc gcgcgccgcg
      301 ccagacggct acaccctcgt gttgaatacc ataccgctgg ttaccaacca ggccatgttc
      361 cccaacctga gctgggaccc gatccgcgac ttcgcggcga tcggcatggt ggccacctcg
      421 ccgcacgtag tggtcgtgcc gggcaaatcg ccgtaccaca gcatcgacga gctggtgcgc
      481 gcggcgcgcg ccagccccgg caggctgacc tacgcctcgg cgggggtggc caccacattc
      541 catctgtgcg ccgagctgtt caaggacacg acccatacct tcatcctgca cgtcccctac
      601 cgcggcggcg gcccggcgct gctcgacacg ctcgccgcgc aggtggacat gagttttccg
      661 acgctggccg cggcggtgcc gcacgtgaag tcgggcgcgc tgcgcgccct ggcggtcacc
      721 gacgtgcggc gctccgcgct gctgcccgat gtgccgacgc tgagggaagc cggcctcaag
      781 gacttccagt tctcgcaatg gcaggcgctg ctcgcgccgg caaagacgcc gccggctatc
      841 gtggcgcggc tcaacgccgc cctgcgcgag gccctcaagt cgaaggacct ggtggagaag
      901 tttcaggcgc aggccttcga cccgttcatc acctcacccg aagaggcggg ggaatacatc
      961 gccgaggagg caaagcgcct ctcgcgcgtc atcaaaacca agggcatcac cgcggactga
     1021 acatggaact cggactgaag gacaagaggg tattgatcac cggcggcagc aagggcatcg
     1081 gcctcgcctg cgcccgggct ttcatcgcgg aaggcgcgcg cgttgccatc gtttcgcgct
     1141 ctcaggccaa ccttgatgcg gcgaagaagg aactgaagaa ttgctattgc atcgcgggcg
     1201 atctcatcga tgcggccgcg gcagcccgcg tcgttgagaa aaccgaaagc gatttcgggc
     1261 cgatcgatgt cctggtcaat tccgccggcg cggcgaagcg caccaacgcc gacgacctga
     1321 cgccgcaggc ctggcgcgcc gcgatggacg ccaagtactt cacctacatc aatgtcatcg
     1381 atccgctggt aaagcgcatg gcggagcgcg gcggcggcgt gatcgtcaac atcatcggct
     1441 cgggcggcaa actcgcctcg ccgatccatc tgccgggcgg cgcggccaat gcggcgctga
     1501 tgctcgccac cgcgggcctc gccaacgcct acgcggcgaa aggcgtgcgc gtggtcggca
     1561 tcaatccggg cctcaccaac accagccgcg tggccgaagg cctcaaggcc gacgcgaagc
     1621 gcgaaggcat ctcggaggac gaggcgctcg cgcgcgcggt caggaagctg ccgctcggcc
     1681 gcctcgccga gccgggcgag atcgccgaca tcgccgtctt cgccgcctcg gcgcgcggac
     1741 gatttttgac aggcgccaac atcagcgcgg acggcgtatc cacgcctacc gttgtgtagt
     1801 tacttgacgc cgcggtaagg ctctgcgagg gtggaaagat gccttcggca cgctttcgcc
     1861 tgcggctttt ctggtaccgg ctcgacaagg aagcctgcgc ggtcgcgctc gtcttcggcc
     1921 tcatcgtcgg cggcttcgcc tacgcggtgc aagccgtcgt cggcgagcgc gaggcggccc
     1981 tggcccgcct gcacgcaaat cacgcccaga acatcgcctg cctcgcgcgc aacgtctact
     2041 tcgaggcgcg cggcgagccc gaggccggcc agtatgcggt cgccgaagtc accatgaacc
     2101 gcaaggcgtc gggccgctac cccaatacgg tctgcggagt ggtctacgaa aggcaggcgt
     2161 tctcatggac cgcggtggac atcctgcccg agcccgaagg cgaagcctgg agccgggcgc
     2221 aggaaatcgc cgaagaggtc tactacggcc gccacgcgcc ggtgctgaac ggcgcgctgt
     2281 tcttccacgc cacctacatc aagccggact gggcgaccga gaagcggcgc gtcgcccgca
     2341 tcggcagcca cattttctac cggtaaaaaa atggcccggc ttgcgccggg ccagatttgc
     2401 agcctactaa ctctcttgca gcttagaagt tgtggcgcaa gccgatggcg atcgacttga
     2461 agtccgcgcc cgccaccgcc ggggtgacct ggcggaagcc ataggtgccg ttggtgtcgt
     2521 tgtcgaccgt cgtgtagaac gcgtacacct tcgtcgcctt ggtgatgttg tagttgtagg
     2581 cgagggtgta ctgcttggcg ccgttgtccg tgcccgggtc ggtatcgttc gccatgccgt
     2641 agttgacatg gaactcgtgc tggcctaccg gcaacatgcc cgagacgcgc cagtacgtgc
     2701 ggtccccgct tccgccgccc gccaggtcgc tcttcgactt ctcgaacagg ccgccgagga
     2761 cgaacgcctt gaagtcgtac agggcgccaa tggtgtacgc cttgtcgtcg ttgcctgtgc
     2821 ccaccgcgag ggccgaggtg ttcttggttt cggcgtacga caccgcgaca tgcagcccgc
     2881 ccttgtcata cgcgccgacc aggccgatgt aattcggctg gtcggtcacg ccggcggcgc
     2941 gcccctcgtt ggccgtcgtg ggtccgaacg agtacgccac gtccgcgctg aacccgctga
     3001 agttcggggt ggtgtaccac agcaggttgt tcatgaaccc ggcgttgccg aagatcgtgg
     3061 ggttcagcag gttgtccgac gacgtgccgg tgtcgtggtt gtgcaagctg atgtagtcgt
     3121 aggtcgcgta gtacaccggg ccgatgctgc ggccggcgcg gatcgtgccg aagccgccct
     3181 gtaggccggc ataactgtcg cggctcgaga acgtgctgcc gctgccgtcg tcgagcttga
     3241 cgcggctttc gacctgccag atcaccttca ggccggtgtt cagctccttg ttgccgcgga
     3301 aaccgatgcg cgaggagttg tcgaccagct cgttggtcga ctggttcggc agggccgtgg
     3361 tgttgctgct gttgctgacc gtaatgtgct cgacgctgac gttgacccgg ccatagacct
     3421 ggatctcggc aggcacctgg gcgagcgccg gcgaggcgca cgccgcggtg acagccgcag
     3481 ccagtagttt tctcttcatg atcatccctt tctgtctgat gttgcgccgt cgggcttgtt
     3541 ttggaggagg ttcccaacgg cgtcgaagcg acctcgcctc gtacgctcac tgtagtgaag
     3601 gctgcaaacc gtcggcaact tgatgttgcg taaaagccgc ttttcgagcc ctggttgttg
     3661 ctacggcgcg acttgttgtt tcgcgtagcg gacggtttgg tcaagaaacg cactgcgtga
     3721 gtgcgacttt tgcccggttt accccccgtt ttgagccagc ctggcgtagc gtccgggggt
     3781 aacgccaaac gcccccttga acaggcgcgc gaagtgcgct tggtcggcga agcccgcctc
     3841 gagcgccaaa tcggcggtgg gtcgcccggc gtttaggcgc gcgcgaacga actccagccg
     3901 gcgcatgagg gagtacctgt aagggctggt gccg
//
LOCUS       DASYJA010000545         5531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_809097, whole genome shotgun sequence.
ACCESSION   DASYJA010000545 DASYJA010000000
VERSION     DASYJA010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809097"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VGO02_11760"
     CDS             complement(<1..386)
                     /locus_tag="VGO02_11760"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HEV7478400.1"
                     /translation="MKDRISQLMDGELDERSAADAIKACGQDAEALQIWRTYHLISDA
                     MRDSRVLSAGFSMRFAERLEAEPTVLAPQRKRAESRTWFAASAAASFAAVALVGWLAF
                     APQPSNAPPPMAQAPVLKPLLEAPQVP"
     gene            complement(391..990)
                     /gene="rpoE"
                     /locus_tag="VGO02_11765"
     CDS             complement(391..990)
                     /gene="rpoE"
                     /locus_tag="VGO02_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HEV7478401.1"
                     /translation="MSDREIDRQLVERAQQGDKKAFGLLVEKYQRKLARLLSRFIRDP
                     AEVEDVTQEAFIKAYRALPAFRGDSAFYTWLYRIGINTAKNYLMALGRRAPTSTEVEA
                     EDAEGFEEGEQLRDINTPESLLLSNEIAETVNATIEKLPEELRKAIQMREIEGMSYED
                     IAQAMDCPIGTVRSRIFRAREAIAEQLRPLLGTSKDKRW"
     gene            complement(1119..1571)
                     /locus_tag="VGO02_11770"
     CDS             complement(1119..1571)
                     /locus_tag="VGO02_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478402.1"
                     /translation="MSAARSLKLSPSHTLAGLIVGAHLCAGAAAIVALPGWAGAALGA
                     ALAGLGLASAWSRALLRSPRSPRTIRLGSGLSPEGAMVDLAGGESSPAQAGGHVSRLM
                     VTLALRRPAHHILVTADMLDRDSFRALRIWALWGKLPGAGVARKQLTA"
     gene            complement(1568..2806)
                     /gene="fabF"
                     /locus_tag="VGO02_11775"
     CDS             complement(1568..2806)
                     /gene="fabF"
                     /locus_tag="VGO02_11775"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV7478403.1"
                     /translation="MTRRRVVVTGLGIVCPIGNTVGEAWDNALAGKPGITRITRFDPA
                     RLACQIAGEVKGFDVSPFMSPKDARRMDTFIHYGMAAGLQAWRDCALQVTPDNAERVG
                     INFGSGIGGLPLIEEMHNELLKNGPRRISPFFIPGTIINMIAGLLSIEIGAKGPNLAM
                     VTACTTSTHCIGEASKSIRYGEADVMIAGGAEAVVTELAIGGFASARALSTRNDDPAT
                     ASRPWDKERDGFVLGEGAGAMVLEEYEHAKARGARIYCELTGYGVSADAHHMTAPPED
                     GDGAFRAMRNALKDASLDATAIDYINAHGTSTPLGDIAETVAVKRMLGAHAAKVAVNS
                     TKSMTGHLLGAAGGVEAVFTALALRDQVSPPTINLRTPDPQCDLDYVPNSARKMKIRA
                     AMSNSFGFGGTNGTLVFARS"
     gene            complement(2861..3097)
                     /gene="acpP"
                     /locus_tag="VGO02_11780"
     CDS             complement(2861..3097)
                     /gene="acpP"
                     /locus_tag="VGO02_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV7478404.1"
                     /translation="MENVEQRVKKIIAEQLGVNEGEIKDNSSFVDDLGADSLDTVELV
                     MALEEEFETEIPDEEAEKITTVQQAIDYIKSHAK"
     gene            complement(3141..3866)
                     /gene="fabG"
                     /locus_tag="VGO02_11785"
     CDS             complement(3141..3866)
                     /gene="fabG"
                     /locus_tag="VGO02_11785"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEV7478405.1"
                     /translation="MSLEGKLALVTGASRGIGQAIAAELAKQGAKVVGTATTADGASG
                     IAQKGIIGKALNVRDAAQCDALVEAIQKELGDILILVNNAGITRDNLALRMKDADWDE
                     VMETNLRAVFRLSRAVMRGMMKARWGRIINITSVVGASGNPGQVNYAAAKAAVVGMSK
                     SLAREIGSRSITVNCVAPGFIDTDMTRALSDEQKKALLGQIPLGRLGKVEDIAAAVAY
                     LASPGADYVTGTVLHVNGGMYMS"
     gene            complement(3863..4789)
                     /gene="fabD"
                     /locus_tag="VGO02_11790"
     CDS             complement(3863..4789)
                     /gene="fabD"
                     /locus_tag="VGO02_11790"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006759768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEV7478406.1"
                     /translation="MKLAMVFPGQGSQAVGMLKGYEGLAGVAEVRAEAAAALGNDFLT
                     LLDEGPAGQLNLTVNTQPAMVTAGYMGYRAWIGSGGPRPAMVAGHSLGEYTALVAAGA
                     LTFADCLPLVRFRAQAMQEAVPEGQGAMAAILNLDDELVRQACAAAGGEVQAVNFNAP
                     GQVVIAGEKAAVARAIEACKTAGAKRALPLPVSAPFHSSLMRPAAERLKDYLQKVDLK
                     PTTITLVNNVDVRVEREPAAIRDALARQAASPVRWVETIRKMVAMGATHIVECGPGKV
                     LAGMTKRIDANVQSLALADRAALEQALQGVKA"
     gene            complement(4786..>5531)
                     /locus_tag="VGO02_11795"
     CDS             complement(4786..>5531)
                     /locus_tag="VGO02_11795"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEV7478407.1"
                     /translation="QIDLIVVATSTPDYIFPSTACLLQAKLGVKGCAAFDVQAVCSGF
                     VYALATADSFMKNGIYGKALVVGAEVFSRILDWNDRGTCVLFGDGAGAVVLAADSKPG
                     IHASVLHADGSQVDMLSVPGNVCKGHITGSPFLQMNGQGVFKYAVKVLDEVARETVAA
                     AGMSLADVDWLIPHQANVRILDATARKLGIAPQKLVVTVDHHGNTSAASVPLALDEFI
                     RAGKIRAGHRVLMEGVGGGFTWGATLATL"
BASE COUNT          821 a         1918 c         1824 g          968 t
ORIGIN      
        1 ggtacctggg gcgcttctag aagaggtttc aagacaggag cttgtgccat gggcggcggt
       61 gcattgctcg gctgcggcgc gaacgccagc cagcccacca gcgccaccgc ggcgaagctc
      121 gcggccgccg aggccgcgaa ccaggtgcgc gactcggccc gcttgcgctg cggcgcaagc
      181 accgtcggct ccgcctcgag gcgctcggcg aagcgcatcg agaagcccgc cgacagcacc
      241 cggctgtcgc gcatggcatc gctgatcagg tgataagtgc gccagatctg gagggcttct
      301 gcgtcctgcc cgcacgcctt gatcgcatcg gcggccgaac gctcgtccag ctcaccatcc
      361 atcaactgcg agattctgtc tttcatgccg ctaccacctc ttgtccttgc tggtccccaa
      421 aagcggccgc agctgctcgg cgatcgcctc gcgcgcccgg aagatcctcg agcgcacggt
      481 gccgatcggg caatccattg cctgcgcgat gtcctcgtaa ctcatgcctt cgatctcgcg
      541 catctgaatc gccttccgca actcctccgg cagcttctcg atcgtggcgt tcaccgtctc
      601 cgcgatctcg ttggacagca acaggctctc gggcgtgttg atgtcgcgca gctgctcgcc
      661 ttcttcgaac ccttcggcat cctcggcctc cacctcggtc gaggtcggcg cccgccgacc
      721 cagggccatc aggtaattct tggccgtgtt gatgccgatc ctgtacagcc acgtgtagaa
      781 cgcgctgtcg ccacggaatg cggggagggc ccggtaggcc ttgatgaacg cttcctgcgt
      841 tacatcttcg acctcggccg gatcgcggat gaagcgcgac agcagccgcg ccagcttgcg
      901 ctggtacttt tcgacaagca ggccaaaagc cttcttgtcg ccttgctgcg cccgctccac
      961 cagctgccgg tcgatttcgc ggtcagacat cggaccgcgc tctcaagccg gtgctcggca
     1021 agggctcccc acccccggac atacagtccg ttctgcaacg cctatgtgcc acagacacac
     1081 gcactttgaa atagttcggg gcagtaagag gccgcggctc aagcagtcag ttgtttcctc
     1141 gccacgccgg cgccggggag cttgccccac agcgcccaga tgcgcagggc gcggaacgaa
     1201 tcgcgatcga gcatgtctgc ggtgaccagg atatggtgcg cggggcgccg taaagcaagc
     1261 gtgaccatca gccggctgac gtgtccccct gcctgggcgg gagagctttc gccgcctgcc
     1321 aggtcgacca tcgccccctc cggcgagagc ccgctcccca gccggatggt gcggggcgag
     1381 cgcggcgagc gcagcagggc gcggctccag gcgctcgcca ggccaagccc ggccagcgcg
     1441 gcgccgagcg cggcccccgc ccagccgggc aacgccacga tggccgccgc cccggcgcac
     1501 aggtgcgcgc cgacgatgag gccggccagg gtatgggacg gtgacagctt caggctgcgg
     1561 gcggcgctca actgcgcgcg aaaaccaggg tgccgttggt cccgccgaag ccgaacgagt
     1621 tcgacatcgc ggcgcggatc ttcatcttgc gcgcgctgtt cggcacatag tcgaggtcgc
     1681 actgcggatc gggcgtgcgc aggttgatgg tcggcggcga gacctggtcg cgcagcgcga
     1741 gcgcggtgaa gacagcctcc accccgccgg ccgcgccgag caggtgcccg gtcatcgact
     1801 tggtggagtt gaccgccacc ttggcggcgt gcgcgccgag catgcgcttc accgccacgg
     1861 tctcggcgat atcgccgagc ggcgtcgagg tgccgtgcgc gttgatgtag tcgatggccg
     1921 tggcatcgag cgaggcgtcc ttcagggcgt tgcgcatggc gcggaacgcg ccgtcgccgt
     1981 cctcgggagg cgcggtcatg tggtgggcgt cggccgatac gccgtagccg gtgagctcgc
     2041 agtagatgcg ggcgccgcgc gccttggcgt gctcgtactc ctccagcacc atcgcgccgg
     2101 cgccttcgcc gagcacgaag ccgtcgcgct ccttgtccca ggggcggctg gcggtggccg
     2161 ggtcgtcgtt gcgcgtggag agagcgcgcg ccgaggcgaa accgccgatc gccagctcgg
     2221 tcaccaccgc ctcggcgccg ccggcgatca tgacgtcggc ctcgccgtag cggatcgact
     2281 tggaggcctc gccgatgcag tgcgtcgagg tggtgcaggc ggtgaccatg gcgaggttcg
     2341 gacccttcgc gccgatctcg atcgacaaga gcccggcgat catgttgatg atggtgcccg
     2401 ggatgaagaa cggcgagatg cgccgcggcc cgttcttgag gagctcgttg tgcatctcct
     2461 cgatcagcgg tagcccgccg atgcccgagc cgaagttgat gccgacgcgc tcggcgttgt
     2521 cgggcgtcac ctgcagcgcg caatcgcgcc acgcctgcag gccggccgcc atgccgtagt
     2581 ggatgaaggt atccatgcgt cgcgcgtcct tcggcgacat gaagggcgag acgtcgaagc
     2641 ccttcacctc gccggcgatc tggcaggcga ggcgcgcggg atcgaagcgc gtgatgcgcg
     2701 tgatgcccgg cttgccggcc agcgcgttgt cccaggcctc gccgaccgtg ttgccgatcg
     2761 ggcagacgat gccgaggccg gtgacgacga ctctgcgacg cgtcatctaa ggtcgccggc
     2821 ccgcgcagcg ggctggaacg tttgcttcac ctattcggtt ttacttggcg tgcgacttga
     2881 tgtagtcgat ggcctgctgg acggtggtga tcttctccgc ctcctcgtcg gggatctcgg
     2941 tctcgaactc ctcctcgagc gccatcacca gctccaccgt gtcgagcgag tcggcgccga
     3001 ggtcgtcgac gaacgaggag ttgtccttga tctcgccctc gttgacgccg agctgctcgg
     3061 cgatgatttt cttgacgcgt tgttctacgt tttccatcgt catccttccc gatttctatg
     3121 gggccaagag gggccgtatt ttacgacata tacatgccac cgttgacgtg caaaaccgtc
     3181 ccggtgacat agtccgcgcc aggcgacgcg agataggcga ccgcggcggc gatgtcctcg
     3241 accttgccga ggcggccgag cggtatctgg ccaagcaggg cttttttctg ctcgtccgac
     3301 agcgcgcgcg tcatgtcggt gtcgatgaag cccggcgcga cgcagttgac cgtgatgctg
     3361 cggctgccga tttcacgcgc cagcgacttg ctcatgccga ccaccgctgc cttcgccgcc
     3421 gcgtagttga cctgcccggg gttgccggag gcgccgacta ccgaggtgat gttgatgatg
     3481 cggccccagc gcgccttcat catgccgcgc atcacggcgc gcgacagccg gaacaccgcg
     3541 cgcaggttgg tttccatcac ctcgtcccag tcggcgtcct tcatgcgcag cgccaggttg
     3601 tcgcgcgtga tgccggcgtt gttcaccagg atcaggatat cgccgagctc cttctggatc
     3661 gcctcgacca gcgcgtcgca ctgggcggcg tcgcggacgt tgagcgcttt cccaatgatt
     3721 cccttttgtg caattccgga agcgccatcg gccgtcgtgg cggtgccgac taccttcgcg
     3781 ccctgctttg cgagctcggc cgcgatcgcc tggccgatac cgcgcgaggc gccggtgacc
     3841 agcgcgagct ttccctcgag gctcatgcct tgactccttg cagcgcctgc tcgagcgccg
     3901 cgcgatccgc cagcgcgagc gactgcacgt tcgcatcgat gcgcttggtc attccggcga
     3961 gcaccttgcc cggcccgcac tcgacgatgt gcgtcgcgcc catcgcgacc atcttgcgga
     4021 tggtctcgac ccagcgcacc ggcgacgccg cctgccgcgc gagcgcgtcg cggatcgccg
     4081 ccggctcgcg ctccacccgg acgtcgacgt tgttcaccaa ggtgattgtt gtcggcttga
     4141 ggtctacctt ttgaaggtag tccttcagtc gttcggctgc cggacgcatc aagctggagt
     4201 ggaacggcgc gctgaccggc agcggcaagg cgcgcttggc gccggccgtc ttgcaggcct
     4261 cgatggcgcg cgccaccgcg gccttctccc ccgcgatcac cacctggccc ggcgcgttga
     4321 agttgacggc ctggacttcg ccgcccgcgg ccgcgcaggc ttgccgcacc agctcatcgt
     4381 ccaggttcag gatcgccgcc atcgccccct gtccctcggg caccgcttcc tgcatcgcct
     4441 gcgcgcggaa gcgcaccagc ggcaggcagt cggcaaaggt gagcgcgccg gccgcgacca
     4501 gcgccgtgta ttcgcccagg ctgtgtccgg cgaccattgc cgggcgcggc ccgcccgaac
     4561 cgatccaggc ccggtagccc atgtacccgg cggtgaccat cgccggctgc gtgttcaccg
     4621 tcaagttgag ctgccctgcg ggcccttcat cgagaagcgt caggaaatcg ttccccaatg
     4681 ccgcggccgc ctcggcgcgt acctccgcga cgccggcaag cccttcgtag cctttgagca
     4741 tgccgacggc ctgcgagccc tgccccggaa ataccatcgc gagcttcata gcgtggcgag
     4801 cgtggcgccc caggtgaagc cgccgccgac gccttccatc agcacgcggt gtccggcgcg
     4861 gatcttcccg gcgcggatga attcgtccag cgcgaggggt accgaggcgg ccgaagtgtt
     4921 gccgtggtgg tcgaccgtca ccaccagctt ctgcggcgcg atgccgagct tgcgcgcggt
     4981 cgcgtcgagg atgcgcacgt tcgcctggtg cgggatcagc cagtcgacgt cggcgaggct
     5041 catgccggcc gcggcaacgg tttcccgggc cacctcgtcg agcaccttga ccgcgtactt
     5101 gaacacgccc tgcccgttca tctgcaggaa aggcgagccg gtgatgtggc ccttgcagac
     5161 gttgcccggc accgaaagca tgtcgacctg gctgccgtcg gcgtgcagca cgctggcatg
     5221 gatgccgggc ttcgagtcgg cggcgagcac cacggcgccg gcgccgtcac cgaacagcac
     5281 gcaggtcccg cggtcgttcc agtcgaggat gcgcgagaac acctcggccc cgacgaccag
     5341 ggctttccca tagatgccat tcttcatgaa actgtcggca gtcgcgagag cgtagacaaa
     5401 cccgctgcac accgcctgca cgtcgaaagc tgcgcagccc ttcaccccga gcttggcttg
     5461 cagcaggcag gccgtgctgg gaaatatgta gtccggcgtc gaagtcgcca ccacgatgag
     5521 atcgatctgc t
//
LOCUS       DASYJA010000546         2713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1171448, whole genome shotgun sequence.
ACCESSION   DASYJA010000546 DASYJA010000000
VERSION     DASYJA010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171448"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VGO02_11800"
     CDS             complement(<1..576)
                     /locus_tag="VGO02_11800"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEV7478408.1"
                     /translation="MIFVLLSLFAAQTAAQEKFPEKPVRFIVPFPPGGGTDALARILA
                     AKLGELWGQQVIVDNRAGAQGNIGTAAGAKAPADGYTITFAHQGALVINPHLYKDTGY
                     DTLKDFAAVARTTEMAFIAVAHPSVPAKDLKELAQYARQNPGKLTFASTSAGPHIVGE
                     LFKLTTRTDLVHVPYKGAGPAINDLLGGQVSL"
     gene            complement(582..1523)
                     /locus_tag="VGO02_11805"
     CDS             complement(582..1523)
                     /locus_tag="VGO02_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7478409.1"
                     /translation="MRKLFWLWLLLAGTPAAAQSNVVRIVVPFAAGGVQDIVARSINA
                     ELGAALGRSVIVENRPGAGGTVGTGSVAKAAPDGTTLVLAAASHTIAGSLYSKLAYDP
                     IADFTGVAHIGSVDYVLMIHADVPARTVREFIAYGKSNPGKLNYATAGNGSATHLSMA
                     YFAGLAGIEMVHIPFKATGEAVNEVLAGRSHAVIAANIGALPYAKDARVRMLGATGAA
                     RSKFLPELPAIAESLPGYEFDSWIALLAPAATPRPVVEQLNAAVAGLLKDPVILGRLD
                     KQGVVPQAMTPDALNALLRADFQKMARVVKASGARID"
     gene            complement(1527..1703)
                     /locus_tag="VGO02_11810"
     CDS             complement(1527..1703)
                     /locus_tag="VGO02_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478410.1"
                     /translation="MKRKYGNALKRETIAEESMRKAAQAAHNNRLSFGTKSRKGSATR
                     GKRRGLAFKAAYQR"
     gene            complement(1751..2545)
                     /locus_tag="VGO02_11815"
     CDS             complement(1751..2545)
                     /locus_tag="VGO02_11815"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEV7478411.1"
                     /translation="MSDGQSSQFAGHDAPTRLRKALKQDEFVLFCQPILALRGGGGFA
                     LAEVLIRQREEEQALLPPGEFLPLFEEHQMMPLLDRWVLVRILQRLAAGCRVPRLAMN
                     VAGQTLLDEEFPGFFAESLKRSKLPAAAIVFEIDENDLVGRPQAVKRFAERIRACGGG
                     LAVSGFGRRLDSFEALERLRPHFVKVDGSITLKITRSAAHEGMLKAVLRMAVSLRIGV
                     IAECVEEQDVLLRLGALGVGYAQGFGVSTPRPLEHLSPRYKAANAA"
BASE COUNT          410 a          921 c          926 g          456 t
ORIGIN      
        1 cagcgagacc tggccgccca gcagatcgtt gatcgccggg ccggcgccct tgtagggcac
       61 gtgcaccagg tcggtcctgg tcgtcagctt gaacagctcg ccgacgatgt gcggcccggc
      121 cgaggtcgag gcgaaggtca gcttgcccgg gttctgccgc gcgtactgcg cgagctcctt
      181 caggtccttc gccggcacgg aaggatgcgc caccgcgatg aacgccatct cggtggtgcg
      241 tgcgactgcc gcgaaatcct tgagggtgtc gtagcccgtg tccttgtaga ggtgcgggtt
      301 gatcaccagc gcgccctggt gcgcgaaggt gatggtgtag ccgtcggccg gcgctttcgc
      361 cccggcggcg gtgccgatgt tgccctgcgc gccggcgcgg ttgtcgacga tcacctgctg
      421 gccccacagc tcgcccagct tcgccgccag gatgcgcgcc agcgcgtcgg tgccgccgcc
      481 cggcgggaac gggactatga aacggacggg tttctcgggg aatttttctt gagccgctgt
      541 ctgggcggca aacaacgaca gcaagacaaa aatcaaagtt ctcaatctat cctcgcgccg
      601 gaggccttca ccaccctggc catcttctgg aaatcggcgc gcagcagcgc gttgagcgcg
      661 tccggggtca tcgcctgcgg caccacgccc tgcttgtcga ggcggccgag gatcaccggg
      721 tccttgagca gcccggccac tgcggcgttg agctgctcga cgaccggccg cggcgtcgcc
      781 gccggcgcga gcagcgcgat ccaggaatcg aactcgtagc ccggcaggga ctcggcgatc
      841 gccggcagct cgggaagaaa cttggagcgc gccgcgcccg tagctcccag catgcgcacg
      901 cgcgcgtctt tcgcgtacgg cagcgcgccg atgttcgcag cgatcactgc gtgcgatcgt
      961 ccggcaagga cctcgttcac cgcctcgccg gtcgccttga aggggatgtg caccatctcg
     1021 atacccgcca gccccgcgaa gtaggccatc gagagatggg tggcgctgcc gttgcccgct
     1081 gtggcgtaat tcaacttgcc cggattcgac tttccatagg caatgaattc acggacggtt
     1141 cttgcgggca cgtcggcatg aatcatcaac acgtagtcca cgctgccgat gtgcgcgacg
     1201 ccggtgaagt cggcgatcgg gtcgtaggcg agcttcgagt agagcgagcc ggcgatggtg
     1261 tggctcgccg cggcgaggac cagggtggtg ccgtcgggcg cggccttcgc caccgagccg
     1321 gtgccgaccg tgccgccggc gccggggcgg ttctcgacga tgacgctgcg cccgagcgcc
     1381 gcgccgagct cggcgttgat ggagcgcgcg acgatgtcct gcacgccccc tgcggcgaag
     1441 ggcacgacga tgcgaacgac gttcgattgc gccgctgccg gcgtgcccgc cagcaacagc
     1501 cacaaccaga acagcttcct caagccctac ctttggtagg cggccttgaa ggcgaggccg
     1561 cggcgcttgc cgcgcgtggc cgagcccttg cgcgacttgg tgccgaagct gaggcggttg
     1621 ttgtgcgcgg cctgcgcggc cttgcgcatg ctctcttcgg cgatggtttc gcgcttgagc
     1681 gcgttgccgt atttgcgttt catctgcgtc ccttgtggcg tttcaagact acgaggggcg
     1741 caatgttacc tcaggcggcg ttcgccgcct tgtatcgggg cgaaagatgc tcgagcgggc
     1801 gcggcgtcga cacgccgaag ccctgggcgt agcccacgcc cagcgccccc aggcgcagca
     1861 gcacgtcctg ctcctcgacg cactcggcga tcacgccgat gcgcagcgac accgccatgc
     1921 gcagcaccgc cttcagcatg ccctcgtggg ctgcgctcct ggtgatcttc agggtgatgg
     1981 agccgtcgac cttgacgaag tgcggccgca gcctttccag cgcctcgaag gagtccaggc
     2041 gccggccgaa gcccgacacc gccaggccgc cgccgcaggc gcggatgcgc tcggcgaagc
     2101 gcttcaccgc ctgcggccgg ccgaccaggt cgttctcgtc gatctcgaac acgatggcgg
     2161 cggcgggcag cttcgagcgc ttgagcgact cggcgaagaa tcccgggaac tcctcgtcga
     2221 gcagggtctg gccggcgacg ttcatggcga ggcgcggcac gcggcagccg gcggcgaggc
     2281 gctgcaggat gcgcaccagc acccagcggt cgagcagcgg catcatctgg tgctcttcga
     2341 acagcggcag gaactccccc ggcggcagca gcgcctgctc ttcctcgcgc tgccggatga
     2401 gcacctcggc gagcgcgaag ccgccgccgc cgcgcagcgc gagaatcggc tggcagaaga
     2461 ggacgaactc gtcctgcttc agcgccttgc gcagccgcgt cggcgcgtcg tgcccggcga
     2521 attgcgagga ttgcccgtcg ctcatttcct cgaagtatgc gacgacgcgc agcgggcagc
     2581 cggactactg cgggactttc ttgcggtatt tcacacccgg caccagtcgg aaattatctt
     2641 ttcgtatatt tcgaccgact ggcgcagccg ctccatgctg cagtactcgt cggtctggtg
     2701 cgccatcgcc gcc
//
LOCUS       DASYJA010000547         3538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_263464, whole genome shotgun sequence.
ACCESSION   DASYJA010000547 DASYJA010000000
VERSION     DASYJA010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263464"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VGO02_11820"
     CDS             <1..347
                     /locus_tag="VGO02_11820"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV7478412.1"
                     /translation="ARQDLRAERSREAMARAIEDLSAGGVPFRAMTEVGMPAERIAAV
                     AEREKVDEIMMGVGRHPAWLRWINPSIAQGVMARTDIGVTVFARGEVGMLQRYAVPAG
                     VAGLAALLLTAE"
     gene            complement(334..807)
                     /locus_tag="VGO02_11825"
     CDS             complement(334..807)
                     /locus_tag="VGO02_11825"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7478413.1"
                     /translation="MPISHIEHFLVAADDIDATRDWYARVLGMKSGPHPDFGFPVHWM
                     YLGDVDIVHIGPSAKQAGEIQKKFLGRTSQDADAGTGALDHIAFRATGLRQMLQHLRA
                     QNVPFTQRRANGQALFQLFLYDPNGVKIELNYAAAEAEGIAPELMASELIKATQQ"
     gene            824..1276
                     /locus_tag="VGO02_11830"
     CDS             824..1276
                     /locus_tag="VGO02_11830"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEV7478414.1"
                     /translation="MAANVYVADAKRLTAAGAKKMMAAAIGGAEKAGIAVTVAIVDAG
                     GNLMLLERMEGGRFHTVHSATTKAVCAASNRRQTTSQGAVGQSLDVAHAIGLALAAGP
                     ERWTAMEGGCPVLVGGECVGGIGVAGGSWEHDQKLAKEAVEAIGAQAA"
     gene            1273..2259
                     /locus_tag="VGO02_11835"
     CDS             1273..2259
                     /locus_tag="VGO02_11835"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV7478415.1"
                     /translation="MTVRVACLGMGWWSDVLADAVQRSGKMTIAACYSRSAEKRIKFS
                     NKYNCRAADSYEEMLRDKSIQAIINTTPNATHLETTRAAAAAGKHVFLDKPIANTIAD
                     ARAITKACRDAKVVLALGYQRRRESHFRLIKQRIGEFGKLVNAEANISRDRLGKIDLD
                     SWRYQAQGMPGGVMLQIGIHYTDVLEYLMGPIKAVSGRFVQLVLPGDNPDVASLVLEH
                     ESGAQSTLNASYASAGEYYLMNIYGKEATAYYDLHNGLRLQRRGSEKVEKIPAGKNDA
                     IVDELDEFAAAVRGEAEPEMDGERSTVSLAVILAGIKSAREGRRVEVEEILA"
     gene            2256..3194
                     /locus_tag="VGO02_11840"
     CDS             2256..3194
                     /locus_tag="VGO02_11840"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7478416.1"
                     /translation="MKCLLILLGLFVSTVSAQDFPHRNPLEITVLFPAGSSADVTARM
                     LAEGMSRALGQRVLVVNRPGAGGAIGYRYVAAQKPDGYALVWNSNSISTTFHSGQLEF
                     NYQAFDAVARVLVESVVVAVRSDARWKNLNDLVAEAKAKPKAISVGHSGIGSHTHISL
                     AALFNATGVQVNEVPFGAAQVVPSLLGGHVDAVVQLPAALSAPVKQGQVRMLAALIPA
                     RDPALPDVPTAKEQGIDVSLEAWRGIAVPKGTPKAVIGALESAIKTSVNSTEFQKGSE
                     NLGVRPAFLGADDFSSLISKEDAELSRLMQQIGLKK"
     gene            complement(3205..>3538)
                     /locus_tag="VGO02_11845"
     CDS             complement(3205..>3538)
                     /locus_tag="VGO02_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEV7478417.1"
                     /translation="ASLNAPLYVRGTFSQPRFSLDPEAAAKGIGAIVIGVLNPLLAVI
                     PLINEGPGKDSPCGELVASALANTKAAAAGRSSAAGATGSASQPELRPSSRPKPEAKP
                     QFQPPEQP"
BASE COUNT          621 a         1151 c         1209 g          557 t
ORIGIN      
        1 ccgcccggca ggatctgcgc gccgagcgca gccgcgaggc gatggcgcgc gcgatcgagg
       61 atctttccgc cggcggcgtg ccgtttcgcg ccatgaccga ggtcggcatg cccgccgagc
      121 gcatcgcggc cgtggccgag cgcgagaagg tcgacgagat catgatgggc gtcggccgtc
      181 atcccgcctg gctgcgctgg atcaatccgt cgatcgcgca gggcgtgatg gcgcgcacgg
      241 acatcggcgt cacggtcttc gctcgcgggg aagtcgggat gctgcagcgc tacgccgtgc
      301 cggcgggagt cgctggcctt gccgccctgc tgcttactgc tgagtagcct tgatgagctc
      361 ggaggccatc agctcagggg cgatgccctc ggcctccgcc gcggcgtagt tcagctcgat
      421 cttgacgccg ttcgggtcgt agaggaaaag ctggaatagc gcctggccat ttgctcgtcg
      481 ttgagtaaat ggaacatttt gggcgcggag gtgttgaagc atttgccgca gccccgtcgc
      541 ccggaacgcg atgtggtcga gcgcgccggt gccggcatcc gcgtcctgcg aggtgcggcc
      601 gaggaatttc ttctggatct cgccggcctg cttcgcgctc ggcccgatgt gcacgatgtc
      661 cacgtcgccg aggtacatcc agtgcaccgg gaagccgaag tccggatgcg gcccggactt
      721 catgccgagc acccgcgcgt accagtcgcg cgtcgcgtcg atgtcgtcgg cggcgaccag
      781 aaaatgctcg atgtgagaaa tcggcatgca ctaatatata acgatggcag caaacgtgta
      841 cgtcgcggac gccaagcggc tcaccgccgc gggggcgaaa aaaatgatgg cggcggcgat
      901 cggcggcgcc gagaaggcgg ggatcgcggt cacggtggcg atcgtcgatg ccggcggcaa
      961 cctgatgctg ctcgagcgca tggagggcgg ccgcttccac accgtgcact cggccaccac
     1021 caaagcggtg tgcgccgcat cgaaccgcag gcagaccacc tcgcagggcg ccgtcggcca
     1081 gtcgctcgac gtcgcgcacg ccatcggcct cgcgctcgcc gcgggtcccg agcgctggac
     1141 cgccatggaa ggcggctgcc cggtcctggt cggcggcgag tgcgtcggcg gcatcggcgt
     1201 cgccggcggc agctgggagc acgaccagaa gctggcgaag gaagcggtcg aggcgatcgg
     1261 cgcgcaagcg gcatgacggt ccgcgtcgcg tgccttggga tgggctggtg gtcggacgtg
     1321 ctcgccgacg cggtgcagcg ctccgggaag atgacgattg cggcctgcta cagccgttcg
     1381 gccgaaaaaa gaatcaagtt ttccaacaaa tacaactgca gggccgcgga ctcatacgag
     1441 gaaatgctcc gcgacaagag cattcaggcg atcatcaaca cgacgccgaa tgcaactcac
     1501 ctggaaacca cccgcgccgc ggccgcggcg ggcaagcacg tcttcctcga caagccgatc
     1561 gccaacacca tcgccgacgc gcgcgccatc accaaagcct gccgcgacgc caaagtggtt
     1621 ctcgccttgg ggtatcaaag gagacgggaa agccatttcc gattgataaa acagagaatc
     1681 ggtgaatttg gaaaactcgt taacgccgag gcgaatatca gccgcgaccg gctgggcaag
     1741 atcgacctcg attcatggcg ctaccaggcg cagggcatgc ccggcggcgt gatgctgcag
     1801 atcggtatcc actacaccga cgtgctcgaa tacctgatgg ggccgatcaa ggcggtcagc
     1861 ggccgcttcg tccagctcgt cctgcccggc gacaaccccg acgtggcgag cctggtgctc
     1921 gagcacgaga gcggcgcgca atccacgctc aacgcgagct atgcctcggc cggcgagtac
     1981 tacctgatga acatctacgg caaggaggcc acggcctact acgatctgca caacggcctg
     2041 cgcctgcaga ggcgcgggtc ggaaaaagtc gagaaaattc ctgccgggaa aaacgacgcc
     2101 atcgtcgacg agctggacga gttcgccgcc gcggtgcgcg gcgaggccga gcccgagatg
     2161 gacggcgagc gctcgacggt ctcgctggcg gtgatcctgg cgggcatcaa gtcggcgcgc
     2221 gaggggcgca gggtcgaggt cgaggagatc cttgcatgaa atgtctcttg attcttctcg
     2281 gcctgttcgt ttccacggtg agcgcgcagg actttcctca tagaaacccg ctcgagatca
     2341 cggtcctgtt ccccgccggc tcgtcggcgg acgtcaccgc gcgcatgctc gccgaaggca
     2401 tgtccagggc cctcggccag cgcgtgctgg tggtgaaccg gcccggcgcc ggcggcgcca
     2461 tcggctaccg ctacgtggct gcgcagaagc ccgacggcta cgcgctggtc tggaactcga
     2521 actcgatctc caccactttc cactccggcc agctcgagtt caactaccag gccttcgatg
     2581 ccgtggcgcg cgtcctggtg gagtcggtgg tcgtcgcggt gcgcagcgat gcgcgctgga
     2641 aaaacctcaa cgacctggtc gcggaggcga aggcgaagcc caaggcgatc tcggtcggtc
     2701 actccggcat cggcagccac acgcacatct cgctcgcggc gctgttcaac gccaccggcg
     2761 tgcaggtcaa cgaggtcccc ttcggcgccg cgcaggtggt cccaagcctg ctcggcggcc
     2821 atgtcgacgc ggtggttcaa ctcccggcag cgctgtcggc gccggtgaag caaggccagg
     2881 tgcgcatgct cgcggccctc atcccggctc gcgaccccgc cctgcccgac gtgccgaccg
     2941 cgaaggagca aggcatcgac gtttcgctcg aggcgtggcg cggcatcgca gttccgaagg
     3001 ggactccgaa agcagtgatc ggcgctctcg aatccgcgat caagacttcc gtcaattcaa
     3061 ccgaatttca gaaaggcagc gaaaacctgg gcgtgcgccc ggcgttcctc ggcgccgacg
     3121 acttcagttc cctgatttcg aaggaggacg cggagctgtc gcgcctgatg cagcagatcg
     3181 gcctgaagaa atagctggaa gcgcctaggg ctgctccggc ggctggaact gcggcttcgc
     3241 ttcaggcttc ggtcgcgagc tgggccggag ctcgggttga gaggcgcttc ccgtcgcccc
     3301 ggccgccgac gagcggccgg ccgcggcggc cttggtattc gccagggcgc tggcgaccag
     3361 ctccccgcag gggctgtcct tgcccggccc ctcgttgatg agcggaatca ccgcaagcag
     3421 cgggttgagc acgcctatca cgatcgcacc gatgcccttg gccgcggcct ccggatcgag
     3481 cgagaagcgt ggctgcgaga atgtgccgcg cacgtagagc ggggcgttca gcgacgcg
//
LOCUS       DASYJA010000548         6275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_172358, whole genome shotgun sequence.
ACCESSION   DASYJA010000548 DASYJA010000000
VERSION     DASYJA010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6275
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172358"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VGO02_11850"
     CDS             <1..312
                     /locus_tag="VGO02_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193768.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HEV7478418.1"
                     /translation="KVEEIASRAKRIFIQDKSKTFNTARVEAFELENLVETAMATIVS
                     AEARKESRGAQARSDFPERDDKSWMKHTLWYKEGNRLDYKPVHLKPLSVESMEPKVRT
                     Y"
     gene            328..1023
                     /locus_tag="VGO02_11855"
     CDS             328..1023
                     /locus_tag="VGO02_11855"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEV7478419.1"
                     /translation="MKLSIYRYDPDKDPKPYMKDYEVTLDHADRMLLDAMVKAKAQDD
                     SLSFRRSCREGVCGSDAMNINGKNGLACVTKIADLPEHVTLRPLPGLPVIRDLIVDMT
                     QFFKQYHSVMPYLINNDPPPERERLQSPQDREELNGLYECILCACCSTACPSFWWNPD
                     KFVGPAGLLQAYRFLADTRDQATAERLDNLEDPYRLFRCHTIMNCVDVCPKHLNPTKA
                     IGKIKEMMVRRAV"
     gene            1023..1244
                     /locus_tag="VGO02_11860"
     CDS             1023..1244
                     /locus_tag="VGO02_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEV7478420.1"
                     /translation="MDQAAKNRLQWKCRRGLLELDLVLQRFLVKHPEDDMLSELLDAA
                     DNDLWDIVSGRSDPLDERYRATVERLRAC"
     gene            1256..2545
                     /gene="gltA"
                     /locus_tag="VGO02_11865"
     CDS             1256..2545
                     /gene="gltA"
                     /locus_tag="VGO02_11865"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEV7478421.1"
                     /translation="MEKTATLKLPDGKTLEFPVLSGSIGPTVVDIRTLYGKSGMFTYD
                     PGFMSTASCSSSITYIDGDAGVLLYRGYPIEQLAQHCDFLEVCYLLLNGELPDRKQKD
                     EFVGIVTHHTMIHEQLARLYQGFRRDAHPMAVMVGVVGALSAFYHDALDINNPQHRII
                     SAFRLVAKMPTIVAMSYKYSIGQPFVYPRNDLSYTANFMRMMFAVPAEEYKVNDVLVR
                     AMDRIFILHADHEQNASTSTVRLCGSSGANPYACIAAGIASLWGPAHGGANEACLEML
                     EEIGDVTKIPEFIGKVKDKNSGVRLMGFGHRVYKNHDPRAKLMRETCHEVLNELGLHN
                     DRLFKLALALEKIALEDEYFVSRKLYPNVDFYSGIVQRALGIPVSMFTCIFSLARTVG
                     WIAQWQEMISDPEYKIGRPRQLYKGAARREVKPLDQR"
     gene            2553..5381
                     /locus_tag="VGO02_11870"
     CDS             2553..5381
                     /locus_tag="VGO02_11870"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEV7478422.1"
                     /translation="MMKQFLDNSYLFGGNAPFIEDLYESYLVNPGSVPEDWREYFDKM
                     QVLPGSAQKDVAHAPIVESFIQRSKKGEFAAPRTMPTEHMAPERLQVAALLLVTQYRI
                     AGSRWATVDPLKRQPRPTVPELESAYYDLSEADLEHVVNAGSFVGIDRTSLRNLIQAL
                     RDTYCRNIGFEYMFISDRAQRVWIQERIEPVRGTPKLAPDLQKRLLAKLTEAEQLERY
                     LHTRYVGQKRFSLEGGESLIPSIDDLIQRAGANGVQEIVVGMAHRGRLNVLVNVLGKM
                     PKELFLEFEGKHVHELPMGDVKYHNGFSSDISTPGGPVHLSLAFNPSHLEIVNPVVEG
                     SVRARQRRRDDKTGDQVLPILVHGDAAFAGQGVVMETLNLSGTRGYGTGGTVHLIVNN
                     QIGFTTSDPRDTRSTTYCTDVAKMVEAPIFHVNGDDPEAVLFVTQLALDYRQRFHKDV
                     VIDIVCFRKLGHNEQDEPFVTQPLMYKKISQHPGTRKLYADKLIAQGVIGPEEPEAMI
                     KSYRDALDAGQHTGNPVLSNFKSKFAVDWAPFLNSKWTDAADTHVPLAELQRLAERIT
                     TPPEGFKLHPTVARVVEARKQMAAGNVPVDWGMAETLAYATLLSNGYDVRLSGQDSGR
                     GTFAHRHAVWHDQDRERWDQGSYVPLSNIGKDQGDFVVIDSVLSEEAVLGFEYGFSTA
                     EPNALVIWEAQFGDFANGAQVVMDQFISSGETKWGRVCGLVQLLPHGYEGQGPEHSSA
                     RLERYLQLCAEHNMQVCVPTNAAQIFHLIRRQMLRPFRKPLVVMTPKSLLRKKEAASS
                     IKELANGSFQTVLPEIDAIEPRQVKRIVACCGKVYYDLLSARRERREDHVAIIRVEQL
                     YPFPHKQFKAEMDRYPAATEVVWCQEEPQNQGAWYQTAHYFRENMREDQKLHFVGRPA
                     SASPAGGYMAKHNERQKALVDQALGKIK"
     gene            5463..>6275
                     /locus_tag="VGO02_11875"
     CDS             5463..>6275
                     /locus_tag="VGO02_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEV7478423.1"
                     /translation="MIIEVKVPQLSESVAEATLLDWHKKEGEAVARDENLIDIETDKV
                     VLELPAPAAGVLVKIIKKSRDAVGSGEVIAQIDTDAKASGVSPAKAPVQPQAPGQKSS
                     PAVMPAAQKLAAEKGVDTSKIQGSGRDGRVTKGDVLSQPEKSAPPAQKQPLQQPPAPV
                     NTDALLSGRPEQRVPMSRLRQRVAERLVQSQSTAAILTTFNEVNMAPVMEMRKKYQER
                     FEKEHGTRLGFMSFFVRAAVHALKRYPVVNASIDGTDIVYHGYFDVGVAVGSP"
BASE COUNT         1246 a         2044 c         1969 g         1016 t
ORIGIN      
        1 aaggttgagg aaattgcatc ccgggctaaa cgaattttca tccaagacaa atcgaaaacg
       61 ttcaatactg cgagggtgga agccttcgag ctcgagaatc tcgtcgagac cgccatggcg
      121 acgatcgtct ccgccgaggc gcgcaaggag agccgcggtg cccaggcgcg ctccgacttc
      181 cccgagcgcg acgacaagag ctggatgaag cacacccttt ggtacaagga aggtaaccgg
      241 ctcgactaca agccggtgca cctgaagccc ctgtcggtcg agtccatgga accgaaggtc
      301 aggacatact agggaatagg aagcccaatg aagctcagca tctatcggta cgatcccgac
      361 aaggacccga agccctatat gaaggactac gaggtcacgc tggaccacgc cgaccgcatg
      421 ctgctcgacg cgatggtgaa ggcgaaggcg caggacgact cgctttcctt ccggcgctcg
      481 tgccgcgagg gggtttgcgg ctccgacgcg atgaacatca acggcaagaa cggccttgcc
      541 tgcgtcacca agatcgccga tctgcctgag cacgtgaccc tgcggccgct gccgggcctg
      601 ccggtgatcc gcgacctgat cgtcgacatg acgcagttct tcaagcagta ccactcggtc
      661 atgccgtacc tgatcaacaa cgacccgccg ccggagcgcg agcggctgca gtcgccgcag
      721 gaccgcgagg agctgaacgg cctctacgag tgcatcctgt gcgcctgctg ctcgaccgcg
      781 tgcccgtcgt tctggtggaa tcccgacaag ttcgtcggcc cggccgggct gcttcaagcg
      841 taccgtttcc tcgcggacac gagggaccag gcaaccgccg agcgcctcga caacctagag
      901 gatccttacc ggctgttccg ctgtcatacc atcatgaatt gcgtcgacgt ctgcccgaag
      961 cacctcaatc ccacaaaggc catcgggaaa atcaaggaaa tgatggtacg gcgcgccgtg
     1021 tgatggatca ggccgccaaa aaccggttgc agtggaagtg ccgacgggga ctgctcgagc
     1081 tcgatctggt tctgcagaga tttctggtga agcacccgga ggacgacatg ctgtccgagc
     1141 tgctcgatgc ggccgacaac gatctttggg acattgtgag cggtcgcagc gatccgctgg
     1201 acgaaaggta tcgcgcgacg gtggagcgct tgcgcgcctg ctgaataggg aatctatgga
     1261 aaagaccgcg acactgaagc tgcccgacgg caagacgctc gagttcccgg tgctctccgg
     1321 gtcgatcgga ccgaccgtcg tcgacatccg caccctgtac ggcaagtccg ggatgttcac
     1381 ctacgacccc ggcttcatgt cgaccgcgag ctgcagctcc tcgataacct acatcgacgg
     1441 cgacgcgggc gtgctgctgt accgcggcta cccgatcgag cagctggcgc agcactgcga
     1501 cttcctcgag gtgtgctacc tgctgctcaa cggcgagctg ccggaccgca agcagaagga
     1561 cgagttcgtc ggcatcgtga cgcaccacac catgatccac gagcagctgg cgcgcctgta
     1621 ccagggcttc cggcgcgacg cgcacccgat ggcggtgatg gtgggcgtgg tgggcgcgct
     1681 gtcggcgttc tatcacgacg cgctcgacat caacaatccg cagcaccgca tcatttcggc
     1741 cttccgcctg gtcgccaaga tgccgaccat cgtcgccatg tcgtacaagt actcgatcgg
     1801 ccagccgttc gtgtatccgc gcaacgacct ttcgtacacc gccaacttca tgcgcatgat
     1861 gttcgcggtg ccggccgagg agtacaaggt gaacgacgtg ctggtgcgcg ccatggaccg
     1921 catcttcatc ctgcacgccg accacgagca gaacgcatcg acctcgacgg tgcgcctgtg
     1981 cggttcctcg ggcgccaatc cttacgcctg catcgccgcc ggcatcgcct cgctttgggg
     2041 gccggcgcac ggcggcgcca acgaggcctg cctggagatg ctggaggaga tcggtgacgt
     2101 caccaagatc cccgagttca tcggcaaggt gaaagacaag aactccggcg tgcgcctgat
     2161 gggcttcggc caccgcgtct acaagaacca cgacccgcgc gccaagctga tgcgcgagac
     2221 ctgccacgag gtgctgaacg agctcggcct gcacaacgat cgcctgttca agctcgcgct
     2281 ggcgctcgag aagatcgcgc tcgaggacga gtatttcgtc agccgcaagc tgtacccgaa
     2341 cgtcgatttc tactccggca tcgtgcagcg cgcgctcggc atcccggtgt cgatgttcac
     2401 ctgcatcttc tcgctcgcgc gcaccgtcgg ctggatcgcg cagtggcagg agatgatctc
     2461 cgacccggag tacaagatcg gccgcccgcg ccagctgtac aagggcgccg cgcgccgcga
     2521 ggtgaagccg ctcgaccaga ggtagaaccg ccatgatgaa acagttcctg gacaactcct
     2581 acctgttcgg tggcaacgcg ccgttcatcg aagacctgta cgagagctac ctggtcaacc
     2641 cgggcagcgt gccggaggac tggcgcgagt acttcgacaa gatgcaggtg ctgccgggca
     2701 gcgcgcagaa ggatgtagcg cacgctccga tcgttgaatc ctttattcaa agatccaaaa
     2761 aaggagagtt cgctgcaccg cgcaccatgc cgaccgagca catggcgccg gagcggctgc
     2821 aggtcgcggc gctgctgctg gtcacccagt accgcatcgc cggctcgcgc tgggcgaccg
     2881 tggacccgct caagcgccag ccgcggccga ctgttcccga gctcgagtcc gcctattacg
     2941 atctttccga agcggacctc gagcatgtgg tgaacgcagg ctcgttcgtc ggaatagata
     3001 gaacttcctt aagaaatctg attcaagccc tgcgcgacac gtactgccgc aacatcggct
     3061 tcgagtacat gttcatcagc gaccgcgcgc agcgcgtctg gatccaggag cggatcgagc
     3121 cggtacgtgg cacgcccaag ctcgcccccg acctgcagaa gcggctgctc gccaagctga
     3181 ccgaggcgga gcagctggag cgctacctgc acacccgcta tgtcgggcag aagcgcttct
     3241 cgctcgaggg cggcgagagc ctgatcccgt cgatcgacga cctgatccag cgcgccggcg
     3301 ccaacggcgt gcaggagatc gtcgtcggca tggcgcaccg cggccggctc aacgtcctgg
     3361 tcaacgtgct cggcaagatg cccaaagagc tgttcctcga gttcgagggc aagcatgtcc
     3421 acgagctgcc gatgggcgac gtcaagtacc acaacggctt ctcgtccgac atcagcacgc
     3481 cgggcggccc ggtgcacctg tccctcgcct tcaacccgtc gcacctggag atcgtcaacc
     3541 cggtggtcga gggctcggtg cgcgcgcggc agcgccgccg cgacgacaag accggcgacc
     3601 aggtgctgcc gatcctggtg cacggcgacg ccgccttcgc cgggcagggc gtggtgatgg
     3661 aaacgctcaa tctttccggt acccgtggat acggcacggg agggactgtt catctgattg
     3721 tgaacaacca gatcggcttt accacctccg acccgcgaga cacccgctcg accacctatt
     3781 gcaccgacgt cgcgaagatg gtcgaggcgc cgatcttcca cgtcaacggc gacgacccgg
     3841 aggcagtgct gttcgtcacg cagctcgcgc tcgactaccg gcagaggttc cacaaggacg
     3901 tggtgatcga catcgtctgc ttccgcaagc tcggccacaa cgagcaggac gagccgttcg
     3961 tgacgcagcc gctgatgtac aagaagatca gccagcaccc cggcacgcgc aagctgtacg
     4021 ccgacaagct gatcgcgcag ggcgtgatcg gccccgagga gccggaggcg atgatcaaga
     4081 gctatcgcga cgcgctcgac gccggccagc acaccggcaa cccggtgctg tccaacttca
     4141 agagcaagtt tgccgtcgac tgggcgccgt tcctcaattc gaagtggacc gacgccgccg
     4201 acacgcacgt gccgcttgcc gagctgcaac ggctggccga gcgcatcacc accccgccgg
     4261 aaggcttcaa gctgcatccg acggtggcgc gggtcgtcga ggcgcgcaag cagatggccg
     4321 ccggcaacgt gccggtcgac tggggcatgg ccgagacgct ggcttatgcc accctgcttt
     4381 ccaacggcta cgacgtgcgc ctgtccggcc aggactccgg ccgcggcacc ttcgcccacc
     4441 gccatgcggt gtggcacgac caggaccgcg agcgctggga ccagggcagc tatgtgccgc
     4501 tctccaacat cggcaaggac cagggcgatt tcgtagtgat cgattccgtt ctttccgagg
     4561 aggcggtgct cggcttcgag tacggcttct ccaccgccga gccgaacgcg ctggtcatct
     4621 gggaagcgca gttcggcgac ttcgccaacg gcgcccaggt ggtgatggac cagttcatca
     4681 gctcgggcga gacgaaatgg ggccgggtgt gcggcctggt ccagctgctg ccgcacggct
     4741 acgaagggca gggaccggag cactcttcgg cgcgcctcga gcgctatctg cagctttgcg
     4801 ccgagcacaa catgcaggtc tgcgtgccga ccaacgcggc gcagatcttc cacctgatac
     4861 ggcgccagat gctgcgcccg ttccgcaagc cgctggtggt catgacgcca aaatccctgt
     4921 tgagaaaaaa agaggctgca tcctccataa aagagctcgc caacggctcg ttccagacgg
     4981 tgcttccgga gatcgacgcg atcgagccga ggcaggtgaa gcgcatcgtc gcgtgctgcg
     5041 gcaaggtgta ctacgacctg ctcagcgcgc ggcgcgagcg gcgcgaggac catgtcgcaa
     5101 tcatccgcgt cgagcagctc tatccgttcc cgcacaagca gttcaaggcc gagatggacc
     5161 gctacccggc cgcgaccgaa gtagtgtggt gccaggaaga gccgcagaac cagggcgcct
     5221 ggtaccagac ggcgcactac ttccgcgaga acatgcgcga ggaccagaag ctgcatttcg
     5281 tgggccgccc cgcgtcggcc agccccgcgg gcggctacat ggccaaacac aacgaaaggc
     5341 aaaaagccct tgtagaccag gccctcggaa aaattaagta gcacccggcg agttactcgc
     5401 tggtttttct caacccggga aaggcttagc cggaaccggg gaaaaactga acgggagttt
     5461 caatgatcat cgaagtaaag gtgccgcaat tgtcggagtc ggtcgccgag gcgacgctgc
     5521 tcgactggca caagaaggaa ggcgaagcgg tcgcgcgcga tgagaacctc atcgacatcg
     5581 agaccgacaa agtggtactc gagctgccgg cgcccgccgc cggcgtcctg gtgaagatca
     5641 tcaagaagag ccgcgacgcg gtcggttcgg gcgaggtcat cgcccagatc gataccgatg
     5701 ccaaggcttc aggggtttct cctgcaaagg cccctgttca gccacaggct ccgggccaga
     5761 aatcctctcc tgccgtcatg cccgcagcgc agaagctcgc ggcggagaag ggcgttgaca
     5821 cttcgaagat ccagggcagc ggccgcgacg ggcgggtgac taagggcgac gtgctctcgc
     5881 agcccgagaa atccgctccc ccggcccaga agcagccgct tcagcagccg ccggcgcccg
     5941 tcaataccga cgcattgctc tcgggccgtc ccgagcagcg cgtgccgatg tcgcgcctgc
     6001 gccagcgggt ggccgagcgc ctggtgcagt cgcagtccac cgccgcgatc ctcacgacct
     6061 tcaacgaggt caacatggcg ccggtgatgg agatgcgcaa gaaataccag gagcgcttcg
     6121 agaaggagca cggcacgcgc ctcggcttca tgtcgttctt cgtcagagcg gcggtgcatg
     6181 ccttgaagcg ctatccggtg gtcaacgcct cgatcgacgg cacggatatc gtctaccacg
     6241 gctacttcga cgtcggcgtt gccgtgggca gcccg
//
LOCUS       DASYJA010000549         1847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2805563, whole genome shotgun sequence.
ACCESSION   DASYJA010000549 DASYJA010000000
VERSION     DASYJA010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2805563"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="VGO02_11880"
     CDS             complement(<1..162)
                     /locus_tag="VGO02_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7478424.1"
                     /translation="MKPLEGKVALVTGAGGGIGRDFALAMAAAGAKVLVNDLGTSVKG
                     EGSDAGPAQK"
     gene            complement(173..946)
                     /locus_tag="VGO02_11885"
     CDS             complement(173..946)
                     /locus_tag="VGO02_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEV7478425.1"
                     /translation="MSDLSQLQIKSMREKVSREEWATRTDLAACYRLVHHFGMDDLVY
                     NHISARVPGEEGHFLINAYGMTYDEITASSLVKIDFDGAVVQDSGTGYGINRAGFVIH
                     SAVHRGRPDVACVIHTHTPAGMAVSAMECGLLPLTQNAMYFAGLGYHDYEGPAVDLDE
                     QKRLVADLGQHEAMILRNHGLLSVGTTIPEAFITMYWLDRACQAQVFALLGKTKLPDN
                     ETIKTTNDRYRPGQRRSIGALEWQGLLRLVERKYPGFRD"
     gene            972..1757
                     /locus_tag="VGO02_11890"
     CDS             972..1757
                     /locus_tag="VGO02_11890"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV7478426.1"
                     /translation="MKTALMAVALAALAACAGAERAAAPAGVPIRFLLSFDDGPAPST
                     AKVLDALAANPVQPGVKAIFFVQTQVAGAEGQQMMRRTHAEGHLLAVHTGTAQGHVSH
                     MALSREELEASLRQARADIADIAGVAPRFVRPPFWFYDDEALACYARVQLAMLLTDIN
                     ALDGVVLGVNLVPGKRLLIRAQLERIKREWQEGAPQPMLDGAAPVIATFHDVNTRTAG
                     NLTEYLQLLVEEARAAGLTLDRRPFYDRREDLERAAGLRARQL"
     gene            1765..>1847
                     /locus_tag="VGO02_11895"
     CDS             1765..>1847
                     /locus_tag="VGO02_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEV7478427.1"
                     /translation="MKVLVAVKRVVDFNVKVRVKSDGSGVET"
BASE COUNT          279 a          614 c          644 g          310 t
ORIGIN      
        1 cttctgcgcc gggccggcgt cgctgccctc gcccttgacc gaggtgccga ggtcgttgac
       61 cagcactttc gcgccggcgg cggccatggc gagcgcaaag tcgcggccga tcccgccccc
      121 cgcgccggtg accagggcga ccttgccctc tagcggcttc aatcgccgaa tctcagtcgc
      181 ggaagcccgg atactttctc tcgaccaggc gaagcaggcc ctgccattcg agcgcgccga
      241 tgctgcgccg ctggccgggc cggtagcgat cgttggtggt ttttatggtt tcgttatccg
      301 gcagcttggt cttgcccaga agggcaaaga cctgtgcctg gcacgcgcgg tccagccagt
      361 acatcgtgat gaacgcctcg gggatggtgg tgccgaccga gagcaggccg tggttgcgca
      421 ggatcatcgc ctcgtgctgg ccgaggtcgg ccaccaggcg cttctgctcg tcgaggtcga
      481 ccgccgggcc ttcgtagtcg tggtagccca ggccggcgaa gtacatggcg ttctgggtga
      541 ggggcagcag gccgcattcc atcgccgaca ccgccatgcc ggcgggcgtg tgggtgtgga
      601 tgacgcaggc gacgtccggg cggccgcggt gcaccgcgct gtgaatgacg aagccggcgc
      661 gattgatgcc gtagccggtg cccgagtcct gcaccacagc gccgtcgaag tcgatcttca
      721 ccaggctgga ggccgtgatc tcgtcgtagg tcatgccgta ggcgttgatc aggaagtggc
      781 cttcctcgcc gggcacccgc gccgagatgt ggttgtagac caggtcgtcc atgccgaagt
      841 ggtggaccag gcggtagcag gcggcgaggt cggtgcgcgt cgcccactcc tcgcgcgaga
      901 ctttttcccg catcgatttg atctgcagct gggataggtc gctcatggcc gttattatgc
      961 ctccccaagt gttgaaaaca gccctgatgg cggtggcgct ggccgcgctg gcggcctgcg
     1021 ccggagccga gcgcgccgcc gcgccggctg gcgttccgat ccgcttcctg ctcagcttcg
     1081 acgacggccc ggcgccttcc actgcgaaag tgctcgatgc cctggccgcg aaccctgtgc
     1141 aaccgggcgt gaaggcgatt tttttcgtcc agacgcaggt ggcgggggct gaggggcagc
     1201 aaatgatgcg ccgcacccac gccgaagggc atcttctggc ggttcacaca gggactgcgc
     1261 aaggccacgt cagccacatg gcgctgtcgc gcgaggagct cgaagcctcg ctgcgccagg
     1321 cgcgggccga catcgccgac atcgcgggcg tcgcgccgcg cttcgtgcgg ccgccgttct
     1381 ggttctacga cgacgaagcg ctggcgtgct acgcgcgcgt ccagctggcc atgctgctca
     1441 ccgacatcaa cgcgctcgac ggcgtcgtgc tcggcgtcaa cctggtgccc ggcaagcgcc
     1501 tcctgatccg cgcccagctg gagcgtatca aacgcgagtg gcaggaaggc gcgccgcagc
     1561 cgatgctcga cggcgcggcg ccggtcatcg ccaccttcca cgacgtcaac acgcgcaccg
     1621 caggcaacct gaccgagtac ctgcagctgc tggtggagga agcgcgcgcc gccggcctga
     1681 cgctcgaccg ccggccgttc tacgaccggc gcgaggacct ggaacgcgcc gcgggcctgc
     1741 gcgcgcggca gctataatcg agcgatgaaa gttctggtgg cggtcaagcg cgtcgtcgac
     1801 ttcaacgtga aggtgcgcgt gaaatcagac ggcagcggcg tggagac
//
LOCUS       DASYJA010000550         3076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_537446, whole genome shotgun sequence.
ACCESSION   DASYJA010000550 DASYJA010000000
VERSION     DASYJA010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537446"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2522)
                     /gene="uvrA"
                     /locus_tag="VGO02_11900"
     CDS             complement(<1..2522)
                     /gene="uvrA"
                     /locus_tag="VGO02_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013428718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV7478428.1"
                     /translation="MKQEQIPGDAIVIRGARQNNLKNLSLALPANELIVVTGVSGSGK
                     SSLVFDTLYAEGQRRYVETFSPYARQFLDRMDKPQVDAVEGIPPAIAIDQTNPVRTSR
                     STVGTMTELNDHLKLLFARAAKLHCRGCGKPVVQDTPGSIYESLKAKPDQRLVVTFPV
                     AIPKNFSEQEIQDLLQRQGYTRIHARAKGLLEVVQDRFRVAGVERARVTEALEAALRF
                     GQGKVNIYSESSEVWRYSSELHCAECDIRYAEPIPALFSFNSPLGACDTCRGFGRVIG
                     VDFGLVVPNEALTLKGGCVRPWQTASFKECQGDLEKYAKKRGIPLDTPWKDLTEKQKY
                     WVLEGEDDWQSWKKSWPGTWYGVRRFFKWLESKAYKMHIRVLLSKYRAYTECTACGGT
                     RLKPDAMLWKLDGKSVHDLVLLPIDKTKAFFDGFKSQDEASDLLLSEMRTRLNYLCNV
                     GLGYLTLDRQSRTLSGGEVQRINLTTALGTSLVNTLFVLDEPSIGLHPRDMGRVIGVM
                     KRLRDAGNTLVVVEHDPQIMFAADRILDMGPGPGERGGEIVFYGPPEKLKAEGTLTGE
                     YLSGRRVAFERNDSPKAPEKFIELSGATEHNLKSVDVKIPLERLVCITGVSGSGKSTL
                     MQDVLYPALRKAKGKPTEAPGAFRALRGAERIDEVVFVDQSAIGKTTRSNPASYVGAF
                     DEIRNIFCKTETARERGYTAGTFSFNSGNGRCPTCGGNGFEHVEMQFLSDVYLRCPDC
                     DGKRFRAEILEVTLKEKSIADVLQMTIREAAAFFDFSKEVKKRLQPLVDVGLEYLRLG
                     QPVPTLSGGEAQRLKLAGYLADANSGSDDLSRLFLFDEPTTGL"
     gene            2641..3042
                     /locus_tag="VGO02_11905"
     CDS             2641..3042
                     /locus_tag="VGO02_11905"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEV7478429.1"
                     /translation="MESQATQFLDLYRTGVRTTSDVMKASLESVERMQNQQLQLIRSA
                     LEENAKSVSQLSEVKSMDELVALQARLTGAQAQRMMDYWSGVWRVAGDNQMAVAKLAA
                     SQVQAVSSGLRDSAQGAANQEKRSEPQRRSA"
BASE COUNT          527 a          953 c         1025 g          571 t
ORIGIN      
        1 agcccggtgg tcggctcgtc gaacaggaac agcctggaaa gatcgtccga gcctgaattc
       61 gcgtcggcca ggtagccggc gagcttgagg cgctgcgcct cgccgcccga cagggtcggc
      121 accggctggc ccaggcgtag gtactccagg ccgacgtcga ccaggggttg cagccttttc
      181 ttcacctctt tcgaaaaatc gaaaaacgcg gcggcctcgc ggatcgtcat ctgcagcacg
      241 tcggcgatcg acttctcctt gagggtcacc tcgaggatct cggcgcggaa gcgcttgccg
      301 tcgcagtcgg ggcagcgcag gtacacgtcg gagaggaatt gcatctccac gtgctcgaag
      361 ccgttgccgc cgcaggtcgg gcagcggccg ttgccggagt tgaagctgaa cgtgccggcg
      421 gtatagccgc gctctcttgc agtttctgtt ttacaaaaga tatttcttat ttcatcaaat
      481 gcgccaacgt agctcgcggg gttggagcgc gtggtcttgc cgatcgccga ctggtcgacg
      541 aacaccacct cgtcgatgcg ctcggcgccg cgcagcgcgc ggaacgcgcc gggcgcctcg
      601 gtcggcttgc ccttcgcctt gcgcagcgcg gggtagagca cgtcctgcat cagcgtcgac
      661 ttgcccgagc cggaaacgcc cgtgatgcac accaggcgct cgagcgggat cttcacgtcc
      721 acgctcttca ggttgtgctc ggtcgcgccg ctcagctcga tgaacttttc gggtgccttt
      781 ggcgaatcgt ttcgttcaaa cgcgacccgg cggcccgaca gatactctcc ggtcagggtg
      841 ccttcggcct tcagcttctc cggcgggccg tagaacacga tctcgccgcc gcgctcgccc
      901 gggcccgggc ccatgtcgag gatgcggtcg gcggcgaaca tgatctgcgg gtcgtgctcg
      961 acgaccacca gcgtgttgcc ggcgtcgcgc aggcgcttca tgacgccgat cacccggccc
     1021 atgtcgcgcg ggtgcaggcc gatcgagggc tcgtcgagga cgaacagcgt gttgacgagc
     1081 gaggtgccga gggcggtggt gaggttgatg cgctgcacct cgccccccga gagcgtgcgc
     1141 gactgccggt cgagcgtgag ataacccagg ccgacgttgc acaggtagtt cagccgcgtg
     1201 cgcatctcgc tgagcagcag atcgctggct tcgtcttgtg atttgaatcc atcgaaaaac
     1261 gccttcgtct tgtcgatggg gagaagcacc aggtcatgca ccgacttgcc gtcgagcttc
     1321 cagagcatgg cgtcgggctt gaggcgcgtc ccgccgcagg cggtgcattc ggtgtacgcg
     1381 cgatacttcg agagcaggac gcggatgtgc atcttgtacg ccttgctctc cagccacttg
     1441 aagaagcggc gcacgccgta ccaggtgccc ggccacgact tcttccagct ttgccagtcg
     1501 tcttcgcctt ccaggaccca gtatttttgc ttttcggtaa ggtccttcca cggcgtgtcg
     1561 agcggaatgc cgcgcttctt cgcgtacttc tcgaggtcgc cctggcactc cttgaagctc
     1621 gcggtttgcc agggtctgac gcagccgccc tttaatgtaa gagcttcatt tggaaccacc
     1681 aatccgaagt ccacgccaat gacgcggccg aagccgcggc aggtgtcgca ggcgccgagc
     1741 ggcgagttga acgagaacag cgccgggatc ggctcggcgt agcggatatc gcattcggca
     1801 caatgcagct cggacgagta gcgccatacc tcactgcttt ccgaatagat gtttactttg
     1861 ccctgcccga aacgcagggc ggcttcgagc gcctcggtca cgcgcgcgcg ctcgacgccc
     1921 gcaacgcgga agcggtcctg gaccacttcg agcaggccct tggcgcgggc gtggatgcgc
     1981 gtatagccct ggcgctgcag caggtcctgg atctcctgct cgctgaagtt cttcgggatg
     2041 gcgaccggga aggtgacgac aagtctctga tccggcttcg ctttcagcga ctcgtagatg
     2101 gagccggggg tgtcctgcac cactggcttg ccgcagccgc ggcaatggag tttcgcggcg
     2161 cgggcgaaga gcagcttcag gtggtcgttc agctcggtca tcgtgccgac cgtcgagcgc
     2221 gaggtgcgca ccgggttggt ctggtcgatg gcaatcgccg gcggaatgcc ctcgaccgcg
     2281 tcgacctgcg gcttgtccat gcggtcgagg aactggcggg cgtaggggct gaaggtctcg
     2341 acgtagcgcc gctgcccctc ggcgtagagc gtgtcgaaga cgagcgagga cttgcccgac
     2401 ccggataccc ccgtgacgac gatgagctcg ttcgccggga gggcgaggct gaggttcttc
     2461 aggttgttct ggcgcgcgcc gcgaatgacg atggcgtcgc cggggatctg ttcctgcttc
     2521 actgactgca attttaatcg ttgtcgtgcc accagcgaca ggaaaaaggc tggccgctgg
     2581 atagaaatcg cgccgcgcac ggactaaatt gaaagaccct acaaaggaag gaactcccaa
     2641 atggaatccc aagcgacgca gttcctggat ctgtaccgta ctggcgtgcg gaccacctcg
     2701 gatgtcatga aagcgtcgct ggaaagcgtc gagcgcatgc agaaccagca gttgcagctc
     2761 atccgctccg ccctggaaga gaacgccaag tcggtcagcc agctgtccga ggtaaagagc
     2821 atggatgagc tggtggcgct gcaggcgcgc ctgacgggcg cgcaggccca gcggatgatg
     2881 gactactgga gcggcgtgtg gcgcgtcgcc ggcgacaacc agatggccgt cgccaagctc
     2941 gcggcgtcgc aggtgcaggc ggtgagcagc gggttacgcg attccgcgca aggtgcggcg
     3001 aaccaggaga agcgctcgga accgcagcgc aggagcgcct agcggccgct catctgccgg
     3061 tttcgccgat gccgag
//
LOCUS       DASYJA010000551         4442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3171129, whole genome shotgun sequence.
ACCESSION   DASYJA010000551 DASYJA010000000
VERSION     DASYJA010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3171129"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1478)
                     /locus_tag="VGO02_11910"
     CDS             complement(<1..1478)
                     /locus_tag="VGO02_11910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV7478430.1"
                     /translation="MAASRKRPAKTRSTVRRSSGAPRYPFVNLSSDWYWEQDKELRFT
                     HVQVGDDLPAAERALAVNLIGRLRWETGLEVEGGWEAHRALLKARKPFRDLLMWRTLE
                     DGSRRYISASGRPVFDKNGKFAGYHGVGRNITEQKRAEARLQASESRLRAIVDNEPEC
                     VKLLDAEGRLLDMNPAGLRMIEAAELGPLLGQCVHDLVAPEHRDAFREIVRRVTGGEE
                     QSLEFEMIGLKGTRRWMEVRAVPLRDESSGATLMLGITRDVTDRKRAETKLRESEARF
                     RALAELSSDWYWEQDTEYRFTRLEGRNVVGQDSGLATRLIGQPRWGGELECEGGWRAH
                     RALLEARQPFYNVLMWRKAADGNLRYVRISGEPVFGADGRFTGYRGVGHEITAEKRAE
                     LLLRLEHNAARVLADAADTATGVRGVLRAICGAESLPCGRYFALDEAAGVLRFEQAWH
                     VSDPDIERIVEASRGMSYAPGQGLIGTAWQRSEPTWSRDMQN"
     gene            complement(1588..2991)
                     /locus_tag="VGO02_11915"
     CDS             complement(1588..2991)
                     /locus_tag="VGO02_11915"
                     /inference="COORDINATES: protein motif:HMM:NF039580.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV7478431.1"
                     /translation="MSEVAVPLWLAVGGGALALWALYEHALMPLLRWMVMHPANQVID
                     DVGKHLRIGIRPFQRTRRQALIHRLLTDPKVQQAAEQYALQHKVSRREAEKRVERYAR
                     ETVPAFNAYLYFRIGYWIGKRVAQALYRVRLGYVDAENLARVESDATVVFVMNHRSNM
                     DYVLAGYLAADQTALSYAVGEWARIWPLSHLIRWMGAYFVRRNSKDELYRKVLERYIV
                     MATEAGVPQAVFPEGGLTRDGRMREPKLGAIDYMMRGFRIDGERDLVFVPLGINYDRV
                     LEDRTLLRSADPEAARRGKVGALGTTLRFAFNNLSLALRSQWYRFGYACVNFGAPVSM
                     REYCRSRSLDFHRLPDEARRREMALLGRHLMDAVGRIVPVLPVPLLATVFVRHGDKAL
                     SELELKAEVERLVEELRARGAHVYVPRRDFDYALNVGLRMLRLRRLVGERDGLYYALA
                     EELPLLRYYANSIAHLA"
     gene            complement(3011..3883)
                     /locus_tag="VGO02_11920"
     CDS             complement(3011..3883)
                     /locus_tag="VGO02_11920"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV7478432.1"
                     /translation="MLHADSARNRLIGIGLVSLCYLIFSVLDGSAKWLVQSMPVVMVV
                     WLRFFIHVVVAGAVLLPVKGWSLVRTNHLRWHLLRAAMFVVMTAANFWALQYLQLTVT
                     SSIFFSSPLIIALLSAQLLGEKLDFGRLAAILSGFAGVLVIVHPWSADFHPAMLLAVM
                     NAVIYAVFMMMTRHLAAYDPPETIQFLPAIGATLILTPFAVAAWEAPDTWLEWALACT
                     LGVFGGVGHYLLALAHRYAPSSVIAPFVYQQVIYMALFGYLVFGDVPAPAVWIGASIV
                     VGSGLYLFARERAR"
     gene            complement(3884..>4442)
                     /locus_tag="VGO02_11925"
     CDS             complement(3884..>4442)
                     /locus_tag="VGO02_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEV7478433.1"
                     /translation="IHRFAFELARKRPRKHLTLVTKSNAQRHGMVLWDEIFYAVAKDF
                     PEVKTERILVDAMTTRMVLNPAAIDTVVASNLHADILSDLAAALSGSLGIAPTANLNP
                     ERKFPSMFEPIHGSAFDITGKGIANPIATFWTTAMMLEHLGEPKAAARLMSAIERTTE
                     EQVFTPDLGGVHDTRAVTNAVKRAL"
BASE COUNT          707 a         1514 c         1543 g          678 t
ORIGIN      
        1 tcgttctgca tgtcccggct ccaggtcggc tcgctgcgct gccaggcggt gccgatcagg
       61 ccctgtcccg gggcgtagct catgccgcgc gaggcttcga cgatgcgctc gatatccgga
      121 tcggaaacgt gccaggcctg ctcgaagcgc agcacccccg cggcctcgtc gagcgcgaag
      181 tagcggccgc agggcaggct ttcggccccg cagatcgcgc gcagcacgcc gcgcaccccg
      241 gtggcggtgt ccgccgcgtc ggccagcacg cgcgcggcgt tgtgctcgag gcgcagcagc
      301 agctcggcgc gcttctcggc ggtgatctcg tggccgacgc cgcggtagcc ggtgaagcgg
      361 ccgtccgcgc cgaagaccgg ctcgccgctg atgcgcacgt agcgcaggtt gccgtcggcg
      421 gccttgcgcc acatcagcac gttgtagaac ggctggcgcg cctcgagcag cgcgcggtgc
      481 gcgcgccagc cgccttcgca ctcgagctcg ccgccccagc gcggctggcc gatgagccgc
      541 gtggcgagcc cgctgtcctg gccgacgacg ttgcggccct ccagccgggt gaagcggtac
      601 tcggtgtcct gctcccagta ccagtcggag gagagctcgg cgagcgcgcg aaagcgcgcc
      661 tcgctttcgc gcagcttggt ctcggcgcgc ttgcggtcgg taacgtcgcg cgtgatgccg
      721 agcatcagcg tcgcgccgct cgactcgtcg cgcagcggca ctgcgcggac ctccatccag
      781 cggcgcgtgc ccttcaggcc gatcatctcg aactccaggc tctgctcctc gccgccggtg
      841 acgcgccgca cgatctcgcg gaacgcgtcc cggtgctccg gcgcgaccag gtcgtggacg
      901 cactggccga gcagcgggcc gagctcggcg gcctcgatca tgcgcaggcc ggcggggttc
      961 atgtcgagca ggcggccttc ggcgtcgagc agcttcacgc actcgggctc gttgtcgacg
     1021 atggcgcgca gccggctttc gctggcttgc agccgcgcct ccgcgcgttt ctgctcggtt
     1081 atgttgcggc cgacgccgtg atagccggcg aattttccat tcttgtcgaa caccggccgg
     1141 ccgctggcgc tgatgtagcg ccggctgccg tcctccagcg tgcgccacat gagcaggtcg
     1201 cgaaacggct tgcgcgcctt gagcagcgcg cggtgcgcct cccagccgcc ctccacctcg
     1261 aggccggtct cccagcgcag gcggccgatc aggttcaccg ccagggcgcg ctccgcggcg
     1321 ggcaagtcgt cgccgacctg gacgtgcgtg aagcgcagct ccttgtcctg ctcccagtac
     1381 cagtcggagg agagattgac gaagggatag cgcggcgcgc ctgagctgcg tcggacagtg
     1441 ctgcgcgtct tggctgggcg tttcctgctg gccgccatgc tgccgtgcct cccccggtgt
     1501 caatctaacg gaggcttatc gctccgtcca gagggttggg caccgaaaaa cccgattgac
     1561 agatagcaac ggaacagccc gttaacttca ggccagatgc gcgatcgagt ttgcgtagta
     1621 gcgcagcagg ggcagctcct cggccagggc gtaatacagg ccgtcgcgct cgccgactag
     1681 ccggcgcagg cgcagcatgc gcaggccgac gttgagcgca tagtcgaagt cgcgccgcgg
     1741 cacgtagacg tgcgcgccgc gcgcgcgcag ctcttcaacc agccgctcga cctcggcctt
     1801 cagctcgagc tccgagagcg ccttgtcgcc gtgccgcacg aacacggtgg cgagcagcgg
     1861 caccggcagc accggcacga tgcgcccgac cgcgtccatc aggtgccggc cgaggagggc
     1921 catctcgcgc ctgcgcgcct cgtcgggcag gcggtggaaa tccaggctgc ggctgcggca
     1981 gtattcccgc atcgacaccg gcgcgccgaa gttcacgcag gcgtagccga agcggtacca
     2041 ctggctgcgc agggcgagcg acaggttgtt gaaggcgaag cgcagcgtgg tgccgagcgc
     2101 gccgaccttg ccgcggcgcg ccgcctcggg gtcggcggag cgcagcagcg tgcggtcctc
     2161 gagcacgcgg tcgtagttga tgccgagcgg cacgaacacc aggtcgcgct cgccgtcgat
     2221 gcgaaagccg cgcatcatgt agtcgatggc gccgagcttg ggctcgcgca tgcgcccgtc
     2281 gcgcgtcagg ccgccctccg ggaacactgc ctgcggcacg ccggcctcgg tcgccatcac
     2341 gatgtagcgc tcgagcacct tgcgatagag ctcgtccttg gagttgcgcc gcacgaagta
     2401 cgcgcccatc cagcggatca ggtgcgacag cggccagatg cgcgcccatt cgcccaccgc
     2461 ataggacagc gccgtctggt cggccgccag gtagccggca agcacgtagt ccatgttgga
     2521 gcggtggttc atgacgaaga ccaccgtggc gtccgattcg acgcgcgcca ggttctccgc
     2581 gtcgacgtag cccaggcgca cgcggtagag cgcctgcgcg acgcgcttgc cgatccagta
     2641 gccgatgcgg aaatacagat aggcgttgaa cgcgggcacc gtctcgcggg cgtagcgctc
     2701 gactcgcttt tctgcttccc gccgggaaac cttgtgctga agagcatatt gctcagccgc
     2761 ctgctgcacc tttggatcgg tcagcagccg gtggatcagc gcctggcgcc gggtgcgctg
     2821 gaacgggcgg atgccgatgc gcagatgctt gccgacatcg tcgatgacct ggtttgccgg
     2881 atgcatgacc atccagcgca gcaggggcat cagggcgtgc tcgtacagcg cccacagcgc
     2941 gagcgctccg cctcccaccg cgagccacag cggtaccgcg acttccgaca tgcccctccc
     3001 ggggaggagt ctaacgcgcc ctctcgcgcg cgaagaggta caggccgctg ccgacgacga
     3061 tcgaggcgcc gatccagacc gccggggccg gcacatcgcc gaataccagg tagccgaaca
     3121 gcgccatgta gatgacctgc tggtagacga acggcgcgat cacgctcgag ggtgcataac
     3181 ggtgggcgag cgcgaggagg tagtggccga ctcccccgaa aacgccgagc gtgcaggcga
     3241 gcgcccactc gagccaggtg tcgggcgctt cccacgccgc gacggcgaac ggcgtgagga
     3301 tgagcgtcgc gccgatggcg gggaggaact ggatggtttc gggcggatcg taggccgcca
     3361 ggtggcgcgt catcatcatg aacacggcgt agatgaccgc gttcatcacg gcgagcagca
     3421 tcgccggatg gaagtccgcc gaccaggggt gcacgatcac cagcacgccg gcgaagccgc
     3481 tcaggatcgc cgccagccgg ccgaagtcga gcttctcgcc caggagctgc gcgctgagca
     3541 gcgcgatgat gagcggcgag gagaagaaga tggacgaggt gacggtgagc tgcaggtact
     3601 gcagcgccca gaagttcgcc gccgtcatga cgacgaacat ggcggcacgc agcaggtgcc
     3661 agcgcaggtg attcgtcctg acgagcgacc accccttcac cggcaggagg acggcgccgg
     3721 ccacgacgac gtggatgaag aagcgcagcc acaccaccat caccaccggc atgctctgca
     3781 ccagccactt cgcgctgccg tccagcacgc tgaagatgag gtagcacagc gacaccagcc
     3841 cgatgccgat cagccggttg cgggcgctat ccgcgtgcag cacctaaagc gctcgtttga
     3901 ccgcgttggt cacggcgcgc gtgtcgtgca cgccgcccag gtcgggggtg aaaacctgct
     3961 cttcagtggt gcgctcgatc gcgctcatca ggcgcgccgc ggccttcggc tcgcccaggt
     4021 gctcgagcat catcgccgtg gtccagaaag tggcgatcgg gttggcgatg cccttgccgg
     4081 tgatgtcgaa ggccgagccg tggatcggct cgaacatcga cgggaacttg cgctcggggt
     4141 tcaggttcgc ggtcggcgcg atgccgagcg agccggaaag cgccgccgcc aggtcggaaa
     4201 ggatgtcggc gtgcaggttg gaggcaacca ccgtgtcgat cgccgcggga ttcagcacca
     4261 tgcgcgtggt catcgcgtcg accaggatgc gctcggtctt cacctctggg aaatccttcg
     4321 ccaccgcgta gaagatttca tcccacagca ccatcccatg tcgctgggca ttcgacttgg
     4381 tcaccagggt caggtgcttg cgcggccgct tgcgcgccag ctcgaaggcg aagcggtgga
     4441 tg
//
LOCUS       DASYJA010000552         7680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_809846, whole genome shotgun sequence.
ACCESSION   DASYJA010000552 DASYJA010000000
VERSION     DASYJA010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7680
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809846"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..579
                     /gene="rplE"
                     /locus_tag="VGO02_11930"
     CDS             34..579
                     /gene="rplE"
                     /locus_tag="VGO02_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEV7478434.1"
                     /translation="MPTPRLQTFFRDTVVPDLMQKFGYKTAMQVPRIEKITVNMGVGE
                     TTSDKKVLDHAVADMTKIAGQKPVVTLARRSIANFKVRAGFPVGCMVTLRGARMYEFL
                     DRLVNIAMPRIRDFRGVSNRAFDGRGNYSLGFKEQIIFPEIEYDKIDTLRGMNIAITT
                     TAKNDDEARALLAAFRFPFKH"
     gene            589..894
                     /gene="rpsN"
                     /locus_tag="VGO02_11935"
     CDS             589..894
                     /gene="rpsN"
                     /locus_tag="VGO02_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEV7478435.1"
                     /translation="MAKLALTLRNQKRRKTVEKFKAKRAALFEVLHDTRATDEAKETA
                     RDKLQKLPRDASPVRLRNRCALTGRPRGVYRKFGLGRNKLRDLALKGEVPGVIKASW"
     gene            905..1300
                     /gene="rpsH"
                     /locus_tag="VGO02_11940"
     CDS             905..1300
                     /gene="rpsH"
                     /locus_tag="VGO02_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEV7478436.1"
                     /translation="MSMSDPVSDMLTRIRNAQMVGHTEVVMPASRLKAAIAQVLKDEG
                     YIEDFAMRDNGAKKELRIGLKYYAGRPVIERLERVSKPGLRVYKGREDIPRIMNGLGV
                     AILSTSRGVMTDRKARADGVGGEILCIVA"
     gene            1308..1841
                     /gene="rplF"
                     /locus_tag="VGO02_11945"
     CDS             1308..1841
                     /gene="rplF"
                     /locus_tag="VGO02_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEV7478437.1"
                     /translation="MSRIAKYPVPLPKGVDVTLAGGSISVKGPLGTIARAADPNVAVE
                     KSGEQIVFKALGDSNHADAMSGTMRALVYNMVTGVTKGYEKKLALVGVGYRAQAQGDK
                     LSLTLGFSHPVVHQMPKGIKVVTPTQTEIVISGIDKQLVGQVAAEVRAHRPPEPYKGK
                     GVRYADETVVLKETKKK"
     gene            1854..2210
                     /gene="rplR"
                     /locus_tag="VGO02_11950"
     CDS             1854..2210
                     /gene="rplR"
                     /locus_tag="VGO02_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEV7478438.1"
                     /translation="MSAKNIARLRRARQTRIRIRENGAVRLTVHRTNGHIYAQLTSPA
                     GDKVLASASTLEKELRGQVKSGSTRKAAEIVGQRIAAKAKEKGIEKVAFDRAGYKYHG
                     RVKALADAARAGGLKF"
     gene            2221..2742
                     /gene="rpsE"
                     /locus_tag="VGO02_11955"
     CDS             2221..2742
                     /gene="rpsE"
                     /locus_tag="VGO02_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEV7478439.1"
                     /translation="MAKIQPRMQQSEERGDGLREKMIAVNRVTKVVKGGRVLGFAALT
                     VVGDGDGGIGMGKGKAREVPVAVQKAMEEARRKLFKVKLKNGTLHHVVKGRHGAARVL
                     MQPAHEGTGIIAGGPMRAVFEVMGVTNVLAKCFGSTNPYNVVRATLNGLQSMSTPAEI
                     AARRGKTVEEITG"
     gene            2739..2915
                     /gene="rpmD"
                     /locus_tag="VGO02_11960"
     CDS             2739..2915
                     /gene="rpmD"
                     /locus_tag="VGO02_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEV7478440.1"
                     /translation="MKKIKVRLLKSTAGTRQSHRDTVRGLGLKHINQVSELVDTPAVR
                     GMIDKVNYLVRIES"
     gene            2932..3351
                     /gene="rplO"
                     /locus_tag="VGO02_11965"
                     /pseudo
     CDS             2932..3351
                     /gene="rplO"
                     /locus_tag="VGO02_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264138.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
     gene            3459..4781
                     /gene="secY"
                     /locus_tag="VGO02_11970"
     CDS             3459..4781
                     /gene="secY"
                     /locus_tag="VGO02_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEV7478441.1"
                     /translation="MATALPNVGKGSRYGDLKRRLLFLLAALVVFRVGAHIPVPGIDP
                     NVLAELFQGQQGGILGMFNMFSGGALSRFTIFALGIMPYISASIIMQLMTAVSPQLDQ
                     LRKEGETGRRKITQYTRYGTVFLALFQATGISIALESQPNLVLDPGLMFRITTVTTLV
                     TGTMFLMWLGEQITERGLGNGISIVIFAGIAAGLPNAIAGTLELVRTGAMHPLTALLI
                     ALAILVVTTVVCFVERGQRKILVNYAKRQVGNRVYGGQSSHLPFKLNMSGVIPPIFAS
                     SLILFPATLLGWFGTAEGLVWLRDFSGMLTPGQPIYVLMYAALIVFFCFFYTALQYNP
                     KETADNLKKSGAFVPGIRPGDQTARYLEKILTRLTLAGAIYVTAVCLLPEFLILRWNV
                     PFYFGGTSLLIIVVVTMDFMSQVQAYVMTHQYESLLRKANFKGGLPVR"
     gene            4781..4999
                     /gene="infA"
                     /locus_tag="VGO02_11975"
     CDS             4781..4999
                     /gene="infA"
                     /locus_tag="VGO02_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV7478442.1"
                     /translation="MAKEDVIEMQGEVVENLPNATFRVKLENGHTIHAFISGKMRMNY
                     IRILPGDKVTVQMTPYDLTKGRITFRAK"
     gene            5010..5123
                     /gene="rpmJ"
                     /locus_tag="VGO02_11980"
     CDS             5010..5123
                     /gene="rpmJ"
                     /locus_tag="VGO02_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEV7478443.1"
                     /translation="MKVMASVKKICRKCKVVRRKGVVRIICSDPRHKQRQG"
     gene            5175..5558
                     /gene="rpsM"
                     /locus_tag="VGO02_11985"
     CDS             5175..5558
                     /gene="rpsM"
                     /locus_tag="VGO02_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEV7478444.1"
                     /translation="MARIAGINIPNHQHAEIALTAIFGIGRARAQAICAAAGVQGTRK
                     IKDLNDAEMERLREHVSRLTVEGDLRREVSMSIKRLMDLGCYRGTRHRKGLPVRGQRT
                     RTNARTRKGPRKAAIKLNVQAGKVA"
     gene            5572..5997
                     /gene="rpsK"
                     /locus_tag="VGO02_11990"
     CDS             5572..5997
                     /gene="rpsK"
                     /locus_tag="VGO02_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEV7478445.1"
                     /translation="MASKNAPTRQELQQTAQARARKKVKKNVSEGIAHVHASFNNTII
                     TITDRQGNTLSWATSGNAGFKGSRKSTPFAAQVAAERAGRAAQECGVKNIEVRIKGPG
                     PGRESAVRALNAVGLKIASIADVTPVPHNGCRPPKRRRV"
     gene            6011..6640
                     /gene="rpsD"
                     /locus_tag="VGO02_11995"
     CDS             6011..6640
                     /gene="rpsD"
                     /locus_tag="VGO02_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEV7478446.1"
                     /translation="MARVLDAKCRLCRREGEKLFLKGEKCFTDKCAVERRAYAPGQHG
                     QKSGMRLSDYGKQLREKQKLRRIYGLLERQFRATYKAAARSKGVTGERLLQLLESRLD
                     SVAYRMGFGISRTEARQVVRHNGVLVNGKRVNIPSYQVKPGDTVEIAGKMKEQLRIKA
                     AAAATESRGIPEWLQVDTKGLKGTYKAMPTRADLPSTINESLIIELYSK"
     gene            6658..7635
                     /gene="rpoA"
                     /locus_tag="VGO02_12000"
     CDS             6658..7635
                     /gene="rpoA"
                     /locus_tag="VGO02_12000"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEV7478447.1"
                     /translation="MPQSGFLKPRIVDVQNLSPNHARVTMEPFERGYGHTLGNALRRV
                     LLSSMPGYAPTEVQIAGVVHEYSTLDNVREDVVDILLNLKGVVFRLHNRAEAILSLKK
                     KGDGPVTAADIEPAHDVEIVNPDHVIAHIASGGKLEIQIKVESGRGYVPGNMRYLPEE
                     TKGIGRIVLDASFSPVRRVSYAVESARVEQRTDLDKLIMDIETNGAIEPEEAIRYAAR
                     VLVDQLSVFAQLEGTAMPTEQPKSPAVDPILLRPVDDLELTVRSANCLKAENIYYIGD
                     LIQRTETELLKTPNLGRKSLNEIKEVLASRGLTLGARLENWPPQGLDKR"
BASE COUNT         1540 a         2520 c         2531 g         1089 t
ORIGIN      
        1 gagccggcca aggcgcgcgg acccgccgag ccgatcccca ctccgaggct gcagacgttc
       61 ttccgtgaca ccgtagtccc cgacctgatg cagaagttcg gctacaagac ggccatgcag
      121 gtgcctcgca tcgagaaaat caccgtcaac atgggcgtcg gcgagaccac ctcggacaag
      181 aaggtgctgg atcacgcggt cgccgacatg acgaagatcg ccggccagaa gccggtggtc
      241 acgctggcgc gtcgctcgat cgccaacttc aaggtgcgcg ccgggttccc cgtcggctgc
      301 atggtcacgc tgcgcggcgc gcgcatgtac gagttcctcg accgcctggt gaacatcgcg
      361 atgccgcgca tccgcgattt ccgcggcgtc tcgaatcgcg ccttcgatgg ccgtggcaac
      421 tacagcctcg gcttcaagga gcagatcatt ttcccggaga tcgagtacga caagatcgac
      481 acgctccggg gcatgaacat cgcaatcacc accaccgcca agaacgacga cgaggcgcgc
      541 gcactgctcg ccgcgttccg cttcccgttc aagcactgag gaccacgcat ggccaagctc
      601 gcactcacgc tccgcaacca gaagcgccgc aagacggtgg agaagttcaa ggcgaaacgc
      661 gctgccctgt tcgaggtgct gcacgacacg cgcgccaccg acgaggcgaa ggaaaccgcg
      721 cgcgacaagc tgcagaagct gccgcgcgac gcctccccgg tgcgcctgcg caaccgctgc
      781 gcgctgaccg gcaggccgcg cggcgtgtac cgcaagttcg gactgggacg caacaagctg
      841 cgcgacctcg cgctcaaggg cgaggtgccc ggcgtgatca aggcgagctg gtaaggagac
      901 atcgatgagc atgagcgatc ccgtctcgga catgctgacc cgcatccgca atgcccagat
      961 ggtcgggcac acggaagtgg tgatgcccgc ctcccggctg aaagccgcca tcgcgcaggt
     1021 gctgaaggac gaaggctata tcgaggactt cgcgatgcgc gacaacggcg ccaagaagga
     1081 gctgcgcatc ggcctcaagt actacgccgg gcggccggtg atcgagcgcc tggagcgcgt
     1141 ctccaagccc gggctgcgcg tctacaaggg ccgcgaggac attccgcgca tcatgaacgg
     1201 cctgggcgtg gcgatcctgt cgacctcgcg cggcgtgatg accgaccgca aggcccgcgc
     1261 cgacggcgtg ggcggcgaaa tcctctgcat cgtagcctga ggccgccatg tcccgcatcg
     1321 caaaatatcc cgtgccgctg cccaaggggg tcgatgtgac cctcgccggc ggcagcatct
     1381 cggtcaaggg cccgctcggc accatcgcgc gcgccgccga cccgaacgtc gcggtcgaga
     1441 aaagcggcga gcagatcgtc ttcaaggcgc tcggcgactc gaatcacgcc gatgccatgt
     1501 cgggcaccat gcgcgcgctc gtctacaaca tggtgaccgg cgtcaccaag ggctacgaga
     1561 aaaagctcgc cctggtgggt gtcggctacc gcgcgcaggc gcagggcgac aagctcagcc
     1621 tgaccctcgg cttctcgcat ccggtggtgc accagatgcc caagggcatc aaggtggtca
     1681 cgcccaccca gaccgagatc gtgatcagcg gcatcgacaa gcagctcgtc ggccaggtgg
     1741 ccgccgaggt gcgcgcccac cgcccgccgg agccgtacaa gggcaagggc gtgcgctacg
     1801 ccgatgagac cgtcgtgctg aaagaaacca agaagaagta acgggaaccg accatgagcg
     1861 ccaaaaacat cgcacggctg cggcgtgcac ggcagacgcg aatcaggatc cgggagaacg
     1921 gcgccgtcag gctgaccgtg caccggacca acggccacat ctacgcgcaa ctcacttcgc
     1981 ccgcgggcga caaggtgctc gccagcgctt cgacgctgga gaaggagctg cgcggccagg
     2041 tgaagagcgg cagcacgcgc aaggccgccg agatcgtcgg ccagcgcatc gccgccaagg
     2101 cgaaggaaaa gggcatcgag aaggtcgcct tcgaccgcgc cggctacaag tatcacggac
     2161 gggtaaaggc acttgccgac gcggctcgcg ccggcggcct gaagttctaa agaggtatct
     2221 atggcgaaga tccaaccgag aatgcagcag tccgaggaac gcggcgacgg gctgcgcgag
     2281 aagatgatcg cggtgaaccg cgtcaccaag gtggtgaagg gcggccgcgt cctcggcttc
     2341 gccgcgctca ccgtggtcgg cgacggcgac ggcggcatcg gcatgggcaa gggcaaggcg
     2401 cgcgaagtgc cggtggcggt gcaaaaagcg atggaagagg cgcgccgcaa gctgttcaag
     2461 gtcaagctga agaacggtac gctgcaccac gtggtgaaag gccgccacgg cgcggcgcgc
     2521 gtgctgatgc agcccgccca cgaaggcacc ggcatcatcg ccggcggccc gatgcgcgcg
     2581 gtgttcgagg tgatgggcgt caccaacgtg ctcgccaagt gcttcggctc gaccaatccc
     2641 tacaacgtgg tgcgcgcgac gctgaacggc ctgcagtcga tgagcacgcc ggccgagatc
     2701 gcggcgcgcc gcggcaagac ggtcgaggag atcacgggat gaagaagatc aaggtgcggc
     2761 tgctgaagag caccgccggg accaggcaga gccaccgcga caccgtgcgc gggctggggc
     2821 tgaaacacat caaccaggtg agcgagctgg tcgacacgcc ggcggtgcgc ggcatgatcg
     2881 acaaggtcaa ctacctggta cggatcgaga gctaaggata aagagcacat catgaaactg
     2941 aacacgctca agcccgcagc aggctcgaag cagtcgcgcc accgcgtcgg gcgcggcatc
     3001 gcctcgggct ggggcaagac cgcgggccgc ggccacaagg gccagcgcgc gcgctccggc
     3061 ggcttccaca aggtgggctt cgagggcggg cagatgccgc tgcatcgccg cctgccgaag
     3121 cgcggcttcc attcgctgac gcgcggcgat gtggtcgagg tgcgcattgc caagctcgaa
     3181 ggcatgaagg ccaccgacat cgacctcgcc gccctcaagg ccgaaggcgt ggtgtcgcag
     3241 caggcgcttg ccgcgaaggt gattctcgcc ggcaagctgt cgcgcaagat cacgctgaag
     3301 gaagtgaagg cctccaaggg cgcgcgcgcc atgatcgagg ctgccggcgg gcgctacgag
     3361 ttcaccgagc ccaaggtcga ggaaaaaccc aagaaagaga aaaaagcgaa gaagcccgcg
     3421 gctgaagaag cgcccaagga cgaggcggcc aagtcttaat ggcgacggcg cttcccaacg
     3481 tcggcaaggg ttcgcgctac ggggatctga agcggcggct gctgttcctg ctcgccgcgc
     3541 tggtggtgtt ccgcgtcggc gcccatatcc cggtgccggg catcgatccg aacgtgctgg
     3601 ccgagctgtt ccagggccag cagggcggca tcctcggcat gttcaacatg ttctcgggcg
     3661 gcgccctgtc gcgcttcacc atcttcgcgc tgggcatcat gccgtacatc tcggcgtcga
     3721 tcatcatgca gctgatgacc gcggtcagcc cgcagctcga ccagctgcgc aaggaaggcg
     3781 agaccggacg gcgcaagatc acgcagtaca cgcgctacgg caccgtgttc ctcgccctgt
     3841 tccaggcgac gggcatctcg atcgcgctcg agtcgcagcc caacctggtg ctcgacccgg
     3901 gcctgatgtt ccgcatcacc accgtcacca cgctggtcac cggcaccatg ttcctgatgt
     3961 ggctcggcga gcagatcacc gagcgcggcc tgggcaacgg catctccatc gtcatctttg
     4021 ccggcatcgc cgccgggctg ccgaacgcga tcgccgggac gctcgaactg gtgcgcaccg
     4081 gcgccatgca cccgctcacg gcgctgctga tcgcgctggc gatcctggtg gtgactaccg
     4141 tggtgtgctt cgtcgagcgc ggccagcgca agatcctggt gaactatgcc aagcgccagg
     4201 taggcaaccg ggtgtacggc ggacagagct cgcacctgcc cttcaagctc aacatgtccg
     4261 gggtcatccc gccgatcttc gccagctcgc tgatcctgtt cccggcgacc ctgctgggct
     4321 ggttcggcac cgccgagggc ctggtgtggc tgcgcgactt ctccggcatg ctcaccccgg
     4381 gacagccgat ctacgtcctg atgtacgcgg cgctgatcgt gttcttctgc ttcttctaca
     4441 cggcgctgca gtacaacccg aaagagaccg ccgacaacct caagaagtcg ggcgccttcg
     4501 tgccaggcat ccggcccggc gaccagaccg cgcgctacct tgaaaaaatc ctgacccgcc
     4561 tcacattagc aggcgcgatc tacgtcaccg ccgtctgcct gctgccggag ttcctgatcc
     4621 tgcgctggaa cgtgccgttc tacttcggcg gcacgtcgct gctgatcatc gtcgtggtaa
     4681 ccatggattt catgtcgcag gtgcaggcct acgtcatgac gcaccagtac gagagcctgc
     4741 tgcgcaaggc caacttcaag gggggcttgc ccgttcgctg atggccaagg aagacgtcat
     4801 cgagatgcag ggcgaggtgg tagagaacct gccgaacgcg acgttccgtg tgaagctgga
     4861 gaacggccac acgatccacg ccttcatctc gggaaagatg cggatgaact acatccgcat
     4921 cctgccgggc gacaaggtga cggtgcagat gaccccgtac gacctgacca agggtcggat
     4981 cacatttagg gcgaagtaag gagattgata tgaaggttat ggcctcagtg aagaagatct
     5041 gccgcaagtg caaggtggtg cggcgtaaag gcgtggtgag gatcatttgc tcggatccgc
     5101 gccacaaaca gcgtcagggt tgataaggcc ttgacaaacc agctataatt tcaggttccg
     5161 ctcaaggcac gctcatggcc cgaattgcag gcataaacat ccccaaccac cagcacgccg
     5221 agatcgcgct gaccgcgatc tttggcatcg gccgggcgcg cgcacaggcg atctgcgccg
     5281 ccgccggcgt gcaggggact cgcaagatca aggacttgaa cgacgcggaa atggagcgtc
     5341 tgcgcgagca cgtctcgcgc ctcacggtgg agggcgacct gcgccgcgag gtgtcgatgt
     5401 cgatcaagcg gctgatggac ctcggctgct atcgcggcac gcgccaccgc aagggcctgc
     5461 cggttcgagg ccagcgcacg cgtaccaacg cgagaacgcg caaggggccg cgcaaggcag
     5521 cgatcaagct gaacgtccag gccggcaagg tcgcgtaacc aggaaaacga aatggcgagc
     5581 aaaaacgcac cgacgcgcca ggaactgcag cagaccgctc aggcccgcgc gcgcaagaag
     5641 gtcaagaaga acgtgtcgga gggcattgcc cacgtccacg cgtccttcaa caacacgatc
     5701 atcaccatca ccgaccgcca gggcaacacg ctgtcgtggg cgacttcggg caacgccggc
     5761 ttcaagggct cgcgcaaatc gaccccgttt gccgcgcagg tggccgcgga gcgcgccggg
     5821 cgcgcggcgc aggaatgcgg cgtgaagaac atcgaggtgc gcatcaaggg ccccggcccg
     5881 ggccgtgaat cggcggtgcg cgcgctgaat gcagttggac tgaaaatcgc cagcatcgca
     5941 gacgtgacgc cggtgccgca caacggttgc cgcccgccga agcgccggcg agtgtagaca
     6001 aggagaacgc atggccagag ttctggacgc caagtgccgc ctgtgccgca gggagggcga
     6061 gaagctcttc ctcaagggcg agaaatgctt caccgacaaa tgcgccgtcg agcggcgcgc
     6121 gtacgctccg ggccagcacg gccagaagag cggcatgcgc ctgtccgact acggcaaaca
     6181 gctgcgcgag aagcagaagc tgcgccgcat ctacggcctg ctcgagcgcc agttccgcgc
     6241 cacctacaag gcggccgcgc gctccaaggg cgtgaccggc gagcggctgc tgcagctgct
     6301 cgagtcgcgc ctggactccg tcgcctaccg catgggcttc ggcatctcgc gcacggaagc
     6361 ccggcaggtg gtgcgccaca acggcgtgct ggtgaacggc aagcgcgtca acattccctc
     6421 ctaccaggtg aagccgggag acacggtgga gatcgcgggc aagatgaagg agcagctgcg
     6481 catcaaggcc gcggccgcag ccaccgagtc gcgcggcatt ccggagtggc tgcaggtgga
     6541 cacgaaaggg ttgaagggca cgtacaaggc gatgccgacg cgcgccgacc tgccctcgac
     6601 catcaacgag agcctcatca tcgagctgta ctcgaagtaa gcaaccagga tccaacaatg
     6661 ccgcaatcgg gatttttgaa gccgcgtatc gtcgacgtcc agaacctgtc gcccaaccac
     6721 gctcgcgtca ccatggagcc gttcgagcgc ggctacggcc acacactcgg caacgcgctg
     6781 cgccgcgtgc tgctttcctc gatgccgggg tacgccccga ccgaggtgca gatcgccggc
     6841 gtggtgcacg agtactcgac gctcgacaac gtgcgcgagg acgtcgtcga catcctgctc
     6901 aacctcaagg gcgtggtgtt ccgcctgcac aaccgcgccg aggcgatcct gtcgctgaag
     6961 aagaagggcg acggcccggt gaccgcggcg gacatcgagc cggcgcacga cgtcgagatc
     7021 gtcaaccccg accacgtcat cgcgcacatc gcctcgggcg gcaagctcga gatccagatc
     7081 aaggtcgagt ccggacgcgg ctacgtgccg ggcaacatgc gctacctgcc ggaggagacc
     7141 aagggcatcg gacgcatcgt gctcgacgcc tcgttcagcc cggtgcggcg cgtttcctac
     7201 gcggtcgagt cggcgcgcgt cgagcagcgc accgacctcg acaagctgat catggacatc
     7261 gagaccaacg gcgccatcga gccggaagag gcgatccgct acgccgcgcg cgtgctggtc
     7321 gaccagctct cggtgttcgc gcagctcgag ggcaccgcga tgccgaccga gcagcccaag
     7381 tcgccggcgg tggacccgat cctgctgcgt ccggtggatg acctcgagct caccgtgcgc
     7441 tcggccaact gcctgaaggc cgagaacatc tactacatcg gcgacctgat ccagcgcacc
     7501 gagaccgaac tgctgaagac gccgaacctc ggccgcaaga gcctgaacga gatcaaggaa
     7561 gtgctggcct cgcgcgggct gacgctcggc gcgcggctcg agaactggcc cccgcaaggg
     7621 ctggacaagc gataagcatt cggcttgtcg ccgtgcaacc cccggtacct gacacggccg
//
LOCUS       DASYJA010000553         2522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_718990, whole genome shotgun sequence.
ACCESSION   DASYJA010000553 DASYJA010000000
VERSION     DASYJA010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718990"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VGO02_12005"
     CDS             complement(<1..369)
                     /locus_tag="VGO02_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7478448.1"
                     /translation="MDGIAAALRNALALIASGDPVLLHIVALSLAVSLSAVFCATLLG
                     LPLGAAIAVTRFPGRQAVIVALNALMGLPPVVVGLLVYLLLSRAGPLGDLGLLFTPGA
                     MVIAQSVLILPIVAALTRQAV"
     gene            409..786
                     /locus_tag="VGO02_12010"
     CDS             409..786
                     /locus_tag="VGO02_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7478449.1"
                     /translation="MAWRAKILIVDDSELSLEVLQAKLADRYDIVATNSPGNALKLAR
                     ETEPDLILCDFDMPEMDGSDVSEALAADEDLRHTPLLFLTAIATTGDLNRMRKQIGGR
                     PAISKDSPIDRIVAKIEALLKVD"
     gene            836..1930
                     /locus_tag="VGO02_12015"
     CDS             836..1930
                     /locus_tag="VGO02_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEV7478450.1"
                     /translation="MRRRSFIQAGAAVSLLGSLPRFARAQQLPFDPKPAGWRTFEITT
                     RVEILKPQGVSRAWVPLPSVESDYQKIGGNSWSGNGQTRLARDGKYGAAMVVGEWPAA
                     EKAPVVEVVSTFSTINRATDFGKRNPAIKIDPATARFATAATEMIPTDGIVRKTAYEI
                     TRGKTTDVDKARAIYEWIVDNTQRNPKTRGCGVGDIKAMLESGDLTGKCADLNALYVG
                     LARSVNLPARDVYGIRVAKSDFGYRSLGAGSPNVTRAQHCRAEVFLTGYGWVPVDPAD
                     ARKVVLEEKPQPTTLADPVVPPVRAKLFGAWEMNWVAYNEAHDIRLPGSSGPALGFLM
                     YPQAETGKDRLDSLDPDNFKYTITSREIKA"
     gene            1942..2307
                     /locus_tag="VGO02_12020"
     CDS             1942..2307
                     /locus_tag="VGO02_12020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HEV7478451.1"
                     /translation="MDSFPQGKESMDTYYKPEHLAKFGSIAEGSKKLADKFFDYYTEV
                     FKAGALSAREKALIALAVSHTVQCPYCIDAYSKESLTQGADLEQMTEALHVAAAIRGG
                     ASLVHGVQMLEHIHKQVMA"
     gene            2304..>2522
                     /locus_tag="VGO02_12025"
     CDS             2304..>2522
                     /locus_tag="VGO02_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_274671340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HEV7478452.1"
                     /translation="MSSPRATTSLRSRAAPLAATAMQLKVLSRLPQENFSQKAGRLSP
                     TGIGIFQINVGKLCNMTCRHCHVDAGPDR"
BASE COUNT          485 a          873 c          812 g          352 t
ORIGIN      
        1 caccgcctgg cgcgtcaacg ccgcgacgat cggcaggatc aggacgctct gggcgatgac
       61 catcgccccc ggcgtgaaca gcaggcccag gtcgcccagc gggccggcgc gggagagcag
      121 caggtagacc agcagcccca ccaccaccgg cggcagcccc atcagcgcgt tcagcgccac
      181 gatcaccgcc tgccttccgg gaaacctcgt cacggcgatc gccgcgccga gcggcagccc
      241 gagcagggtc gcgcaaaaga ccgccgacag gctgacggcc aggctgagcg cgacgatgtg
      301 cagcaggacc ggatcgcccg atgcgatcag ggcgagcgcg ttgcgcagcg ctgcggcaat
      361 gccgtccatt tagcgtatct ttgcatagag cgagcaagga ggcggaccat ggcctggcgg
      421 gcgaagatcc tgatagtcga cgacagcgag ctgtccctgg aagtcctgca ggcgaagctt
      481 gccgatcgct acgacatcgt cgccacgaac tctcctggca acgcgctcaa gctggcgcgc
      541 gagaccgagc ccgacctgat cctgtgcgac ttcgacatgc cggagatgga cggcagcgac
      601 gtctccgagg cgctggcagc cgacgaggac ctgcggcaca cgccgttgct gttcctcacc
      661 gccatcgcca cgaccggcga cctgaaccgg atgcgcaagc agatcggcgg ccggccggcg
      721 atctcaaaag actcgccgat cgaccggatc gtcgctaaga tcgaggcgct gctgaaagtc
      781 gactagcagc cgcccttata attgggcgct cgtcgcaacg atccagagga aaatcatgcg
      841 cagacgttca ttcatccagg ccggcgcggc ggtgtcgctg ctcggctccc tcccccgctt
      901 cgcgcgcgcc cagcagctgc cctttgaccc gaagccggcc ggctggcgca ccttcgagat
      961 caccacgcgg gtcgagatcc tcaaaccgca gggcgtatcg cgcgcctggg tcccgctgcc
     1021 gtcggtcgaa agcgactatc agaagatcgg cggcaattcc tggtcgggca acgggcagac
     1081 gcgcctcgcg cgcgacggca agtacggcgc ggcgatggta gtcggcgagt ggcctgccgc
     1141 cgagaaggcg ccggtggtcg aggtggtgag caccttctcc accatcaacc gcgcgactga
     1201 tttcggcaag cgcaacccgg cgatcaagat cgatccggcg accgccaggt tcgccaccgc
     1261 cgcgaccgag atgattccca ccgacggcat cgtgcgcaag accgcctacg agattacgcg
     1321 cggcaagacc accgacgtcg acaaggcgcg cgccatctac gagtggatag tcgacaacac
     1381 ccagcgcaac ccgaagacgc gcggctgcgg cgtgggcgac atcaaggcga tgctcgaaag
     1441 cggcgacctg accggcaagt gcgccgacct gaacgcgctc tacgtggggc tggcgcggtc
     1501 ggtaaacctg ccggcgcgcg acgtgtacgg catccgcgtc gccaagagcg acttcggcta
     1561 ccgcagcctc ggcgccggca gcccgaacgt cacccgcgcg cagcactgcc gcgccgaggt
     1621 gttcctcacc ggctacggct gggtgccggt cgatccggcc gacgcgcgca aggtggtgct
     1681 ggaggagaag ccgcagccga ccacgctcgc cgatccggtg gtgccgccgg tgcgcgccaa
     1741 gctgttcggt gcctgggaga tgaactgggt cgcgtacaac gaggcgcacg acatcaggct
     1801 gcccggctcc agcggtcccg cgctcgggtt cctcatgtat ccgcaggccg agaccggcaa
     1861 ggaccggctc gacagcctcg acccggacaa cttcaagtac acgatcacat cgcgggagat
     1921 caaggcgtag caggcagtac catggattcc ttccctcaag gcaaggaatc catggacact
     1981 tactacaagc ccgagcacct ggcgaagttc ggcagcatcg ccgaaggcag caagaagctc
     2041 gccgacaagt tcttcgacta ctacaccgag gtgttcaagg cgggcgccct gtcggcgcgc
     2101 gagaaggcgc tgatcgcgct ggccgtctcg cataccgtgc aatgccccta ttgcatcgac
     2161 gcgtactcca aggaatccct gacacagggc gccgacctcg agcagatgac cgaggcgctg
     2221 cacgtcgccg cggcgatccg cggcggcgcc tcgctggtgc acggcgtgca gatgctcgag
     2281 cacatacata agcaggtaat ggcctgagca gccctcgcgc cacgacttcg ctgcgctcgc
     2341 gcgcggcgcc gctagctgct acggcaatgc agctgaaggt gctttcgcgc cttcctcagg
     2401 aaaatttttc gcaaaaagcg ggaaggctgt ccccgaccgg gatcggcatc ttccagatca
     2461 acgtcggcaa gctctgcaac atgacctgcc gccactgcca cgtcgacgcc ggccccgacc
     2521 gc
//
LOCUS       DASYJA010000554         6304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1173650, whole genome shotgun sequence.
ACCESSION   DASYJA010000554 DASYJA010000000
VERSION     DASYJA010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173650"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="VGO02_12030"
     CDS             complement(<1..176)
                     /locus_tag="VGO02_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEV7478453.1"
                     /translation="MSRIFNFSAGPAMLPAEVLSRAGDEMLDWRGSGMGVMEMSHRGK
                     EFISIYEETERDLR"
     gene            complement(179..2827)
                     /gene="gyrA"
                     /locus_tag="VGO02_12035"
     CDS             complement(179..2827)
                     /gene="gyrA"
                     /locus_tag="VGO02_12035"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV7478454.1"
                     /translation="MENFAKETLPISLEEEMRRSYLDYAMSVIVGRALPDVRDGQKPV
                     HRRVLFAMHESNNVWNRPYVKCARIVGEVLGKYHPHGDTATYEALVRMAQDFSMRYML
                     IDGQGNFGSVDGDSAAAYRYTECRLEKIASEILADIDKETVDFGPNYDGKELEPGVLP
                     TRVPNLLVNGSSGIAVGMATNIPPHNLGEVTDACLHLLANPHSAIEELIKLMPAPDFP
                     TAGFIYGLTGVHEGYRTGRGRVVMRARTHFEDIGKGDRQAIIVDELPYQVNKKTLLEK
                     LAELVTDKRLEGISDLRDESDKSGMRMVIELKRGEIPDVVLNNLYKITQLQDAFHMNM
                     VALVEGQPRLLSLKDLLESFLSHRREVVTRRTVFDLRKARERGHVLEGLAVALSNVDE
                     VIALIKKAPSPVEAKVGLMSRAWGSDLVTNLIGKNPVAQFRPEGLAENFGLKPEGYFL
                     SDAQAQAILELRLQRLTGLEQDKIRDEYREVIEVIRDLLDILAKPARVTRIIGDELAA
                     LKEAFADKRRSEIVTVAEDISIEDLIAPQDMVVTFSHGGYVKSQPLADYRAQRRGGRG
                     KAATAMKEDDFIERLFVAHSHDYLLCFSDRGQLYWLKVYEVPAGSRTARGKPIVNMFP
                     LAEGEKITAVVAVKEFDENHYVFMATAQGTVKKTSLAEFSRPRPSGIIAVGLDEGDYL
                     IGAALTDGKYDVMLFSSGGKAVRFEEGEVRPMGRQAGGVRGMKLGEEQNVVCMLAAKV
                     DENADSAKSVLTATENGFGKRTAIAEYPRHGRGGQGVIAIQASERNGAVIGAVLVDDN
                     DEVMLISTGGVLIRTSVAQIREMGRSTQGVTLISLDEGERLAGLERIEERDLSNGNGG
                     NGSHGENGETPHDEPPPSGEPPPTVH"
     gene            2937..3494
                     /locus_tag="VGO02_12040"
     CDS             2937..3494
                     /locus_tag="VGO02_12040"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEV7478455.1"
                     /translation="MHTRTLLATACVVAFFGCATQEAPAPAPAPAPVPAPAPAAAPAP
                     APAPVAKPAPEAPKKPALVNIASTELFEFNKAVLTADARGLLEREVIGKLKDLGTIRY
                     VNVNGHADRLGTSTYNQKLSEKRADAVRAYLVSKGVDAKSVETFGFGKTLPVKSCPEQ
                     KQRKELIECLAPNRRVVVEIQGTPR"
     gene            3440..4816
                     /locus_tag="VGO02_12045"
     CDS             3440..4816
                     /locus_tag="VGO02_12045"
                     /inference="COORDINATES: protein motif:HMM:NF006549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HEV7478456.1"
                     /translation="MPGAQSPRGGRDPGHAKIAADPLLIRARWVVPVEPAGAVLENHA
                     VAVRDGKIEAVLPAAQAGARFPGYEEMVLEDHVLLPGLVNAHTHAAMSLMRGLSDDLP
                     LMRWLQEHVWPAEAKHVSPQFVRDGTLLACAEMLRGGITCFNDMYFFPEAAFEAASAA
                     GMRVALGLIVVEFPTAYASDPEDYLRKGLALRDRLRDRPMVSFCLAPHAPYTVSDASF
                     RKIGTLAAELDLPVHVHLHETAEEIARSAAEHGVRPLERLRRLGLVAPNLIAVHAVHL
                     EDAEIELLGRHGCSVAHCPSSNLKLASGFAPVHAMLKNNINVALGTDGAASNNRLDLF
                     QEMRTAALLAKAVSGDAEAMPAHAALRAATLGGAQALGLAALTGSIEPGKAADLVAVA
                     LRAPELAPCYDPVSHLVYAAGREHVSDVWISGEHRVRDGALLPEVFAGLDTRWRMWQN
                     ALKSHADS"
     gene            4866..5393
                     /locus_tag="VGO02_12050"
     CDS             4866..5393
                     /locus_tag="VGO02_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEV7478457.1"
                     /translation="MNRNTWLWLAALAFAAGCATQEAPPPAAAPAPVPAPAPAPAAPK
                     PAPAPEAAKPKPVAEKVTFAADVLFDFDKSVIKPDGKSKLDDLANKMKGINLEVVIAI
                     GHADSIGSDAYNQALSVRRAEAVKAYFVSKGIEGNRVYTEGKGEKQPVADNKTAEGRA
                     KNRRSEIEVIGTRKN"
     gene            5469..5618
                     /locus_tag="VGO02_12055"
     CDS             5469..5618
                     /locus_tag="VGO02_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478458.1"
                     /translation="MAENLKGKTMTVEWWIDVVLIALISVTVVQNMGLRDRLKDIEAK
                     LDRQK"
     gene            5671..>6304
                     /gene="ubiG"
                     /locus_tag="VGO02_12060"
     CDS             5671..>6304
                     /gene="ubiG"
                     /locus_tag="VGO02_12060"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEV7478459.1"
                     /translation="MNADPAELEKFSQLAHRWWDPEGEFRPLHDINPLRLEWIDRHAS
                     LKGKSVLDVGCGGGILTEAMARCGATVTGIDLSEKALKVAELHLHESGLKITYEMVSA
                     EDFAGRHAGAFDVVTCMELLEHVPEPHSMVAACARLVRPGGQVFFSTINRNPKSYLFA
                     VVGAEYVLGLLPKGTHDYARFIKPSELARYSRENNLRPDQLIGMTYNPITR"
BASE COUNT         1059 a         2128 c         2036 g         1081 t
ORIGIN      
        1 tcgcgcaggt cgcgctcggt ttcctcgtag atcgagatga actccttgcc gcggtggctc
       61 atctccatca cgcccatgcc gctgccgcgc cagtcgagca tctcgtcgcc ggcgcgggat
      121 agcacctcgg cggggagcat cgccggcccc gcgctgaaat tgaatattcg agacatcttc
      181 agtggacggt ggggggaggc tcgcccgaag gtggcggctc gtcgtgcggc gtctcgccgt
      241 tctcgccgtg gctgccgttg cctccattgc cgttgctcag gtcgcgttcc tctatgcgct
      301 ccaagccggc aagtctctcg ccctcgtcga gggagatcaa cgtcaccccc tgcgtcgagc
      361 ggcccatctc gcggatctgc gccacgctgg tgcggatcag cacgccgccg gtggagatca
      421 gcatcacctc gtcgttgtcg tcgaccagca ccgcgccgat gaccgcgccg ttcctctccg
      481 aggcctggat ggcgatcacg ccctggccgc cgcggccgtg gcgcgggtac tcggcgatcg
      541 ccgtgcgctt gccaaaaccg ttctcggtcg cggtgagaac cgacttcgcg gagtccgcat
      601 tttcgtcgac cttggccgcc agcatgcata ccacgttctg ctcctcgccg agcttcatgc
      661 cgcgcacgcc gccggcctgg cggcccatcg gccgcacctc gccttcctcg aagcgcaccg
      721 ccttgccccc ggaggagaac agcatgacgt cgtatttgcc gtcggtgagc gcagcgccga
      781 tcagatagtc gccctcgtcc aggccgaccg cgatgatgcc gctcgggcgc gggcgcgaga
      841 actcggcgag cgaggtcttc ttcaccgtgc cctgcgccgt cgccatgaac acgtagtgat
      901 tctcgtcgaa ctccttcacc gccaccaccg cggtgatctt ctcgccctcg gcgagcggga
      961 acatgttgac gatcggcttg ccgcgcgccg tgcggctgcc cgcaggcacc tcgtacacct
     1021 tcagccagta gagctggccg cggtcggaga agcacagcag gtagtcgtgc gagtgcgcga
     1081 cgaacaggcg ctcgatgaag tcgtcttcct tcatcgcggt ggcggccttg ccgcgcccgc
     1141 cgcgcctctg cgcccggtag tcggcgagcg gctgcgactt cacgtagccg ccgtgcgaga
     1201 acgtaaccac catgtcctgc ggcgcgatca ggtcctcgat cgagatgtcc tcggcgacgg
     1261 tgacgatctc gctgcgccgc ttgtcggcga aggcttcctt cagcgcggcc agctcgtcgc
     1321 cgatgatcct ggtgacgcgc gccggcttgg cgaggatgtc gagcaggtcg cggatcacct
     1381 cgatcacctc gcggtactcg tcgcggatct tgtcctgctc gaggccggtg aggcgctgca
     1441 ggcgcagctc gaggatcgcc tgcgcctgcg cgtcggacag gaaatagccc tcgggcttca
     1501 agccgaaatt ctcggctaat ccctccggac ggaattgcgc gaccggattt tttccgatca
     1561 agttggttac cagatcagaa ccccaggcgc gcgacatcag cccgaccttc gcctccaccg
     1621 gagagggagc cttcttgatc agcgcgatga cctcgtccac gttggagagc gccaccgcca
     1681 ggccttccag cacgtggccg cgctcgcgcg ccttgcgcag gtcgaacacg gtgcggcgcg
     1741 tgacgacttc gcgccggtgc gagaggaacg actcgagcag gtccttcagc gagagcaggc
     1801 gcggctggcc ctcgaccagc gccaccatgt tcatatggaa ggcgtcctgc agctgcgtga
     1861 tcttgtacag gttgttcagc accacgtcgg ggatctcgcc gcgcttgagc tcgatcacca
     1921 tgcgcatgcc ggacttgtcg gactcgtcgc gcaggtcgga gatgccttcc aggcgcttgt
     1981 cggtgaccag ctcggccagc ttctcgagca gcgtcttctt gttcacctgg tagggcagct
     2041 cgtcgacgat gatcgcctgg cggtcgccct tgccgatgtc ctcgaaatgg gtgcgcgcgc
     2101 gcatcaccac gcggccgcgg cctgtgcggt agccctcgtg caccccggtc aggccgtaga
     2161 tgaagcccgc ggtcggaaaa tccggcgccg gcatcagctt gatcagctcc tcgatcgcgc
     2221 tgtgcgggtt ggccagcagg tgcaggcagg cgtccgtcac ttcgcccagg ttgtgcggcg
     2281 gaatgttggt ggccatgccg acggcgatgc ccgaggaccc gttcaccagg aggttgggca
     2341 cgcgggtcgg caacacgccg ggctcgagct ccttgccgtc gtagttcggg ccgaagtcga
     2401 ccgtttcctt gtcgatgtcg gccaggatct cggaggcgat cttctccagc cggcactcgg
     2461 tgtagcggta ggccgccgcg gaatcgccgt cgaccgagcc gaaattgccc tggccgtcga
     2521 tcagcatgta gcgcatcgag aagtcctgcg ccatgcgcac cagcgcctcg taggtcgcgg
     2581 tatcgccgtg cggatggtac ttgcccagca cctcgccgac gatcctggcg cacttcacat
     2641 agggacggtt ccagacgttg ttcgactcgt gcatcgcgaa cagcacccgc cggtggaccg
     2701 gtttctggcc gtcgcgcacg tccggcaggg cgcggccgac gatcacgctc atggcgtaat
     2761 cgaggtagga gcgccgcatc tcctcttcga gcgagatggg cagcgtttct ttggcgaaat
     2821 tttccatcga agagagccga aattttatca cgcaagcgcc ggttttcttt cgcaaaacgg
     2881 tcgcgttttg tgcttaaatc tgccgcgtac gacaggccaa aaaggggagg agaaaaatgc
     2941 acaccagaac actgctcgcg acggcatgcg tggtcgcttt cttcggttgc gccacacagg
     3001 aagcgcccgc gccggctccg gcacccgcac cggttccggc gcccgcgccc gccgccgctc
     3061 cggcgcccgc gcccgcgccg gtcgcgaagc ccgcgcccga agcgccgaag aagcccgcgc
     3121 tcgtcaacat cgcgtcgacc gagctgttcg agttcaacaa ggccgtgctc accgccgacg
     3181 cgcgcggcct gctcgagcgc gaagtcatcg gcaagctgaa ggacctcggc acgatccgct
     3241 atgtcaacgt caacggccac gccgaccgcc tgggcacctc gacctacaac cagaagcttt
     3301 ccgagaagcg cgccgacgcg gtgcgcgcct acctcgtctc gaagggcgtc gacgcgaaga
     3361 gcgtcgagac cttcggcttc ggcaagaccc tgccggtcaa gtcctgcccg gagcagaagc
     3421 agcgcaagga gctgatcgag tgcctggcgc ccaatcgccg cgtggtggtc gagatccagg
     3481 gcacgccaag atagcggccg accccctcct catccgcgcc cgctgggtcg ttccggtaga
     3541 gcccgcaggc gcggtgctcg aaaaccacgc cgtcgcggtg cgcgacggca agatcgaggc
     3601 ggtcctgccg gcggcgcagg ccggcgcgcg ctttcccggt tacgaagaaa tggtattgga
     3661 agaccatgtc ctgcttcccg ggctggtcaa cgcgcatacc cacgccgcga tgtcgctgat
     3721 gcgcggcctg tcggacgact tgccgctcat gcgctggctg caggagcacg tctggccggc
     3781 cgaggcgaag cacgtctcgc cgcagttcgt gcgcgacggc acgctgctcg cctgcgccga
     3841 gatgctgcgc ggcgggatca cctgcttcaa cgacatgtac ttcttcccgg aggctgcttt
     3901 cgaggcggcc tcggccgcgg gcatgcgggt cgcgctcggc ctcatcgtcg tcgaatttcc
     3961 gaccgcgtac gcgtcggatc cggaagacta cctgcgcaag ggccttgcgc tgcgcgaccg
     4021 cctgcgcgac cggccgatgg tttcgttctg ccttgcgccg cacgcgccct atacggtgtc
     4081 cgacgcgagc ttccgcaaga tcggcacgct cgccgccgag ctcgacctgc cggtgcacgt
     4141 gcacctgcac gagaccgccg aagagatcgc gcgctcggcc gccgagcacg gcgtgcgccc
     4201 gctcgagcgc ctgcgccggc tcgggctggt ggcgccgaac ctgatcgcgg tgcacgccgt
     4261 gcacctcgag gacgccgaga tcgagctcct cggccgccac ggctgctcgg tggcgcattg
     4321 cccctcgtcc aacctcaagc tcgcgagcgg ctttgccccg gttcatgcca tgctgaagaa
     4381 caacatcaat gtcgcgctcg gcaccgacgg cgcggcgagc aacaaccggc tcgacctgtt
     4441 ccaggagatg cgaaccgcgg ccctcctcgc caaggcggtc tccggcgacg ccgaagcgat
     4501 gccggcccac gccgcgctgc gggccgccac gctcggcggc gcgcaggcgc tcggcctggc
     4561 cgcgctcacc ggctcgatcg agccgggcaa ggcggccgac ctggtcgccg tggcgctgcg
     4621 cgcgcccgag ctggcgccct gctacgaccc ggtatcgcat ctcgtctatg ccgcggggcg
     4681 cgagcacgta agcgacgtct ggataagcgg cgagcatcgc gtgcgcgatg gcgcgctgct
     4741 gccggaggtg tttgccggct tggacactcg ttggaggatg tggcagaatg ctttgaagag
     4801 tcatgcagac tcttgatacg aataagtttt cagacaagca agagacaaag gtgggaggaa
     4861 aaaatatgaa caggaatact tggctgtggc tggcggcgct cgcttttgcc gcgggttgcg
     4921 cgacgcaaga agcaccgccc ccggcagccg cgcccgctcc ggtgcccgca ccggctccgg
     4981 cacccgcggc gcccaagccc gcgccggctc cggaagctgc caagcccaag cccgtcgccg
     5041 agaaggtcac cttcgccgcc gacgtgctgt tcgacttcga caagtcggtc atcaagcccg
     5101 acggcaagtc gaagctcgac gacctggcta acaagatgaa aggcatcaac ctcgaggtgg
     5161 tgatcgccat cggccacgcc gactcgatcg gcagcgacgc gtacaaccag gcgctctccg
     5221 tgcggcgcgc cgaggcagtc aaggcctact tcgtgtcgaa gggcattgaa ggcaaccgcg
     5281 tctacaccga gggcaagggc gagaagcagc cggtcgcgga caacaagacc gccgaaggcc
     5341 gcgccaagaa ccgccgctcg gagatcgaag tcatcggcac gcgcaagaac tgatcgcccg
     5401 gcgcgatcga aaaacagccc cgcttcggcg gggctttttt ttgcgatatt ggactttacg
     5461 ttgcgggaat ggcagaaaac ctgaagggga aaacaatgac tgtggaatgg tggattgatg
     5521 tggtgttgat agcgctgatt tccgtaaccg tcgttcagaa catggggctt cgagacaggc
     5581 tgaaggacat agaggcaaag cttgatcgcc aaaaatagat cgctgcgtga agtctgatat
     5641 cgtctttttt ttggccctac aatcggcagc gtgaacgccg accccgccga gctagagaaa
     5701 ttcagccagc tcgcccaccg ctggtgggat ccggagggcg agttccggcc gctgcacgac
     5761 atcaacccgc tgcgcctgga gtggatcgat cggcatgcga gcctgaaagg taaatcagtg
     5821 ctcgatgtgg gttgcggcgg cggcattctt accgaagcaa tggcccgctg cggcgccacg
     5881 gtcacgggca tcgacctatc ggagaaggcg ctcaaggtgg ccgagctgca cctgcacgaa
     5941 agcggtttga agatcaccta tgaaatggtc agcgccgagg acttcgccgg gcggcacgcc
     6001 ggcgcgttcg acgtcgtcac ctgcatggag ctgctcgagc atgtgcccga gccgcacagc
     6061 atggtggcgg cctgcgcgcg gctggtgcgg cccggcgggc aggtgttctt ttccaccatc
     6121 aaccgcaatc cgaagtcgta tctctttgcc gtggtcggcg ccgagtacgt gctagggctg
     6181 ctgccgaaag gcacgcacga ctacgcgcgc ttcatcaagc cctccgagct cgcgcgctat
     6241 agtcgagaaa acaacctgcg gccggaccag ctcatcggca tgacctacaa cccgatcact
     6301 cggc
//
LOCUS       DASYJA010000555         8000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_174640, whole genome shotgun sequence.
ACCESSION   DASYJA010000555 DASYJA010000000
VERSION     DASYJA010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8000
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174640"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VGO02_12065"
     CDS             <1..615
                     /locus_tag="VGO02_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7478460.1"
                     /translation="VFGRYFGISQREMAGRIPALLEFAGLAARSAARINTLSGGMKRR
                     LTLARALVNDPQLIVMDEPTTGLDPQARHLIWERLRQLTQQGKTLVLTTHFMEEAERL
                     CQRLAIMDHGRIIAQGSPRSLIAEHTERQVVEVHGPGVEDWMELAKRLAPRVERVGDT
                     VFCYAENVDAILQDLSAKSDLTYLHRAASLEDVFLKLTGRDLRD"
     gene            615..1403
                     /locus_tag="VGO02_12070"
     CDS             615..1403
                     /locus_tag="VGO02_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7478461.1"
                     /translation="MARRNYCALPSLSLRWIAVWRRNVLVWQKLAIPSILGNLADPLI
                     MLFGLGYGLGAMVGELQGSSYFAFLAGGFVATSSMYAATFEALYSAFSRMHVQRTWEA
                     IVNAPVTLDDVLAGEYAWAATKALSAGACMLIIVAAFGYARFPHLLLALPVILLIGLA
                     FAGMGLAINALARGYDFFSYYMTLVLTPMMMLSGVFFPLEQMPAAVQAGAKVLPLYHG
                     VAIVRPLLSGEMPSALLLHVCVLAAYAAAGFYLATVLTRRRLLK"
     gene            1403..1819
                     /locus_tag="VGO02_12075"
     CDS             1403..1819
                     /locus_tag="VGO02_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEV7478462.1"
                     /translation="MLWVKSLHIIFMVTWFAGLFYLPRLFVYHAMASDTVSLERFKVM
                     ERKLYWGIMTPGAVLTIVFGLWLWLQWFRPATGWLHAKLALVVLLVGYHLWCGKLLRD
                     FADGRNTKSHVWLRWFNEVPVVILFATVFLVVLKPF"
     gene            1800..2945
                     /locus_tag="VGO02_12080"
     CDS             1800..2945
                     /locus_tag="VGO02_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEV7478463.1"
                     /translation="MSSSRSESFFASAPRGLESLLLQELSSFGAEGAKETPGGVAFSG
                     SWETCYRANLWSRIASRVLWKVGEFQYSNEQNLYEAAKAVDWTQYFAVQKKIRVNVTA
                     QKSPLKSLEFATLKVKDAVCDRFREKVGSRPDVDRANPDVRIHVFLEEAKGILYLDTS
                     GEALFKRGWRMDIAEAPIRENLAAGIIMLSGWKFDQPLLDPMCGGGTLLSEAAAMARG
                     RAPGAKRSFGFEKLNIFDAKLWERVRKERTVEAVEPRLYGSDTDPEALQAARRNLGAA
                     GVERWVKLEQSDVLERAAPEAAGVMLANPPYGERIGSADELAALYPKLGDALKKRFAG
                     WNCFFLTADLRLPKLIRLQPSRRTPLWNGSLECRLYEFRIISGSNRR"
     gene            complement(2925..4130)
                     /locus_tag="VGO02_12085"
     CDS             complement(2925..4130)
                     /locus_tag="VGO02_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV7478464.1"
                     /translation="MQTDVQCCISGGGPAGMMLGLLLARAGLKVMVLEKHRDFLRDFR
                     GDTIHPSTLELLHELGLLEAFLKLPHQRVSELQAQFGDLSFPVADFSRLPVRCPFIAL
                     IPQWDFLDFIADHAALYPGFRLMMQAEVTSLIEENNRIVGIRGTTAQGPLEVRSDLVV
                     GADGRHSTVRERAKLEVRDFGAPIDVLWFRLSRLPSDPSATMARFGGGRLIVMLNRGD
                     YWQCAFVIAKGSLEALRSRGLEAFRVRLSAAVPFAADRMAEIASWDQLKLLTVQVNRL
                     RQWSRPGLLCIGDAAHAMSPVGGVGINLAIQDAVAAANILYKGDFGSAALRAVQRRRE
                     FPTRLTQAFQVAVQNNILMRVLEDEAPVEAPLAVRLLARWPILRRIPARMVGLGARPE
                     HIASPAVAT"
     gene            complement(4140..5099)
                     /locus_tag="VGO02_12090"
     CDS             complement(4140..5099)
                     /locus_tag="VGO02_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEV7478465.1"
                     /translation="MRLIRIGISGWRYEPWRGVFYPKDLPQRRELEFAARHFPTLEIN
                     GSFYSLQRPEYYERWHAQAPPGFVFAVKGSRYITHLRRLKEIDKPLANFFASGVLALK
                     EKLGPFLWQFPPMFPFKPDRLADFFEKLPTTTRDALALARKRDSRLKGRSRLAIDADR
                     PLRHAVEVRHPSFVSKAFVSLLREHDIALVVADTAGKFPRMFDVTADFVYVRLHGDVQ
                     IYKSGYTPRALDGWAKRIRAWDRAGKDVYVYFDNDVKVRAPFDALNLMHRLGLAWDPA
                     PEARGDDPPFRVHAAGSRIARLKPAYGPRVTGGNPAWENLGRR"
     gene            complement(5090..5842)
                     /gene="surE"
                     /locus_tag="VGO02_12095"
     CDS             complement(5090..5842)
                     /gene="surE"
                     /locus_tag="VGO02_12095"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEV7478466.1"
                     /translation="MRILITNDDGVYSPGIAALAQVAAAFGEVRVVAPDVEMSSASSS
                     ITSSRPVTYKHTPLNGIDAYRVNGTPADCVTLGVSLWEKVDLVLSGVNIGTNLGNAIW
                     HSGTLAGARQATLLGLRGIALSAPATDRDAPSFELLKPWAATVLKQLLEAPELTLVNV
                     NFPDKAPRGTRWTCQSVRHYDGKVVPAKDPMGRTHYWYTVVPVEGTEEDSDRRAIEDG
                     YVSMTPLRLDLTDYEKLEKVRGLEAWKKLRFA"
     gene            5866..6783
                     /locus_tag="VGO02_12100"
     CDS             5866..6783
                     /locus_tag="VGO02_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEV7478467.1"
                     /translation="MRVTLIHNPAAGREGAGDARKLEKLLRRFGHDVRYHSSKDKGLR
                     RILKKRADLVAVAGGDGTVGKVTRRMVSRDVPLAVLPSGTANNVARSLGLVDRPFEEL
                     VRGWDQARRVKLDVGIAAGPWGERYFVEGVGAGLFADLLTRQGSDQDQRRKKRRGNPV
                     ERALKRLKEAAESCEPVDIRARLDGEDISGSYLLLEAVNLRYVGPNLHLAHDSQPGDG
                     QFDVIMATEAERSRLVHYLEHWQENRERLAMLPSRRGKRLQIEWTGFPLHIDDRLEPK
                     EDAEPDEMAGLVEARIAHAVEFLVPAPSR"
     gene            complement(6793..>8000)
                     /locus_tag="VGO02_12105"
     CDS             complement(6793..>8000)
                     /locus_tag="VGO02_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478468.1"
                     /translation="YYFREFGKPLSQYPFLTAPFRLLASWLGWLRLPLPEPFLLGVDK
                     VVADERESFNVILFDRLFEHGVWYYFAVLWFLKTPIAIMAASVAAAWVGWRTQAFRQP
                     GMKLLFFQWALYLLYFSFFFHAQLGYRYVLMLVPMTCLFLAVGLKDWWIKPRGAYVAA
                     AIAVFSLAELVPYYGHTLAFSNSFLLPKKDAYRVLADSNLYWNEYHAQIPGLLLERGV
                     QASINPVHVLPGKNAIDSFELSGVLWVPRRQHKWARDKLETSGHMKHVIFLYDVSERD
                     FSSFLDENRRYPAPRLATDCEGSSGTSVPIAARGEGWMLLCANARELTDVSLSVRSGT
                     VSAGHVNGEGRCIDDTVRKQQEIWFRLEPGRHVLCLSTDGGLEGSWDVRRGAASFQRA
                     KVGTNFNAR"
BASE COUNT         1341 a         2599 c         2741 g         1319 t
ORIGIN      
        1 gtgttcgggc gctatttcgg catttcccag cgggagatgg ccgggcgcat cccggcgctg
       61 ctggagttcg ccggcctggc ggcgcgcagc gcggcccgca tcaacacgct ttccggcggc
      121 atgaagcgcc ggctcacgct cgcccgcgcc ctggtgaacg acccgcagct catcgtcatg
      181 gacgagccga ccaccgggct cgatccgcag gcgcgccacc tgatctggga gcggctgcgc
      241 cagctcacgc agcagggcaa gacgctggtg ctgaccacgc acttcatgga ggaggccgag
      301 cgcctgtgcc agcgcctggc gatcatggac cacggccgca tcatcgcgca gggctcgccg
      361 cgctcgctca tcgccgagca caccgagcgg caggtggtgg aggtgcacgg ccccggcgtc
      421 gaggactgga tggagctggc gaagcggctc gcgccgcgcg tcgagcgcgt cggcgatacg
      481 gtcttctgct atgccgaaaa tgtggacgcg attttgcagg atctttctgc aaaatccgat
      541 ctgacctatc tgcatcgcgc ggcgagcctg gaagacgtgt tcctcaagct gaccggccgc
      601 gatctgaggg attgatggcg cgccgcaact actgcgctct cccgtcgctc agcctgcgct
      661 ggatcgccgt ctggcgccgc aacgtgctgg tatggcagaa gctcgccatt ccctcgatcc
      721 tcggcaacct ggccgacccg ctgatcatgc tgttcggcct gggctacggc ctgggcgcga
      781 tggtcggcga gctgcagggc agctcgtatt tcgcgttcct cgccggcggt ttcgtcgcca
      841 cctcgagcat gtacgcggcg actttcgagg cgctctactc ggcgttctcg cgcatgcacg
      901 tgcagcgcac ctgggaggcg atcgtcaacg cgccggtgac gctcgacgac gtgctggccg
      961 gcgagtacgc ctgggcggcc accaaggccc tgtccgccgg cgcctgcatg ctgatcatcg
     1021 tcgccgcctt cggctacgcg cggtttccgc acctgctcct cgcgctgccg gtgatcctgc
     1081 tgatcggcct ggcgttcgcc ggcatggggc tcgcgatcaa cgcgctcgcc cgtggctatg
     1141 acttcttcag ctactacatg acgctggtgc tgacgccgat gatgatgctc tcgggcgtgt
     1201 tcttcccgct cgagcagatg cccgccgcgg tgcaggcggg ggccaaggtg ctgccgctgt
     1261 accacggcgt ggccatcgtg cgcccgctgc tttccggaga gatgccgagc gcgctgctgc
     1321 tgcacgtctg cgtgctcgcc gcctatgcgg ctgccggctt ctacctcgcc accgtgctca
     1381 cccggcgtag actgctcaaa taatgctctg ggtcaaatcg ctccacatca tcttcatggt
     1441 gacgtggttc gcgggcctgt tctacctgcc ccgcctgttc gtctaccacg cgatggcgag
     1501 cgataccgtc tcgctcgagc gcttcaaggt aatggagcgc aagctctact ggggcatcat
     1561 gaccccgggc gcggtgctca ccatcgtctt cggcctctgg ctctggctgc agtggttccg
     1621 gccggccacc ggctggctgc acgccaagct cgcgctggtc gtgctgctcg tcggctacca
     1681 cctgtggtgc ggcaagctgc tgcgcgactt cgccgacggg cgcaacacga aaagccacgt
     1741 ctggctgcgc tggttcaacg aggtgccggt cgtgatcctc ttcgccacgg tgttcctggt
     1801 tgtcctcaag ccgttctgag tccttcttcg cgagcgcacc tcgcgggctg gaatcgctcc
     1861 ttctccagga actttcttct tttggcgccg agggcgcgaa agaaacgccc ggaggggtcg
     1921 cgttcagcgg gagttgggaa acgtgctacc gcgcgaacct gtggtcgcgc attgcgtcgc
     1981 gcgtgctgtg gaaggtgggg gaattccagt acagcaatga gcagaatctg tatgaggcgg
     2041 cgaaggcggt ggactggacg cagtactttg ccgtgcagaa gaaaattcgc gttaacgtca
     2101 ccgcgcagaa gagcccgctg aagagcctgg agttcgccac gctgaaggtg aaggacgcgg
     2161 tgtgcgaccg cttccgcgag aaggtcggca gccgtcccga cgtggaccgg gcaaaccctg
     2221 atgtgcgcat ccacgttttt ctcgaggaag cgaagggcat cctgtacctc gacacctccg
     2281 gcgaggcgct gttcaagcgc ggctggcgca tggacatcgc cgaggcgccg atccgcgaga
     2341 acctggcggc gggcatcatc atgctgtcgg gctggaagtt cgaccagccg ctgctcgacc
     2401 cgatgtgcgg cggcggcacg ctgctctcgg aggcggcggc gatggcgcgc gggcgcgcgc
     2461 cgggtgcgaa gcggtctttt ggttttgaga aactaaacat ctttgatgcg aagttatggg
     2521 agagggtgag gaaggaaagg accgtcgaag cggtggagcc gcgtctctac ggcagcgaca
     2581 cggacccgga ggcgctgcag gcggcgcgcc gcaacctggg cgccgccggc gtggagcgct
     2641 gggtgaagct cgagcagagc gatgtgctcg agcgtgccgc gccggaggcc gccggggtga
     2701 tgctggccaa cccgccctac ggcgagcgca tcggcagcgc cgacgagctg gccgcgctct
     2761 atccgaagct cggcgatgcc ctgaagaaga ggttcgccgg ctggaactgc tttttcctca
     2821 ccgccgacct gcgcctgccc aagctgatcc ggctgcagcc gtcgcggcgc acgccgctgt
     2881 ggaacggctc gctcgagtgc cgcctttacg agttccgcat catttcaggt agcaaccgca
     2941 ggtgatgcga tgtgctcggg gcgggcgccg agcccgacca tgcgcgccgg aatgcgccgc
     3001 aggatcggcc agcgcgccag aagccggacg gcgagcgggg cctcgaccgg cgcctcgtcc
     3061 tccagcaccc gcatgaggat gttgttctgc accgcgacct ggaaggcctg cgtgagccgg
     3121 gtgggaaatt cgcgccggcg ctgaacggcg cggagcgccg cgctgccgaa gtctcccttg
     3181 taaaggatat tggccgccgc gaccgcgtcc tggatggcga ggttgatgcc gacgccgccg
     3241 acgggcgaca tggcgtgcgc ggcatcgccg atgcatagca gcccgggtcg gctccactgc
     3301 cgcaggcggt tcacctggac cgtgaggagc ttcagctggt cccatgaggc gatctcggcc
     3361 atgcggtctg cggcgaacgg caccgcagcc gagaggcgta cgcggaaggc ctcaaggcct
     3421 cggctgcgga gcgcctccag cgagcccttg gcgatgacga acgcgcactg ccagtagtcg
     3481 ccgcggttca gcatcacaat gaggcggccg ccgccgaagc gcgccatggt cgcggacggg
     3541 tcgctcggca ggcgcgacag gcggaaccag agcacatcga tcggtgcgcc gaagtcccgc
     3601 acttcgagct tggcccgctc gcgcaccgtc gaatggcggc cgtcggctcc gaccaccagg
     3661 tcgctgcgca cctccagtgg accctgcgcg gtggtgcccc tgattccgac aatcctgttg
     3721 ttttcttcga tgaggctcgt gacctcggcc tgcatcatca ggcgaaaccc cggatatagc
     3781 gcagcatggt cggcgatgaa gtccaggaaa tcccattgcg ggatgagcgc gatgaacggg
     3841 cagcgcaccg gcaggcggct gaaatcggct accgggaagg acagatcgcc gaactgcgcc
     3901 tgcagctcgc tcaccctttg gtgaggcagc ttgaggaacg cttcgagcag gccgagctcg
     3961 tgcagcagct cgagcgtcga ggggtggatg gtgtcgccgc gaaaatcgcg caggaagtcg
     4021 cgatgcttct ccagcaccat taccttgagg ccggcgcgcg cgaggagcag cccgagcatc
     4081 atcccggcgg ggccgccgcc ggagatgcag cactgcacgt cggtttgcac ccggacagtc
     4141 tagcgccggc cgaggttctc ccaggcggga ttgccgccgg tgacgcgcgg cccgtaggcc
     4201 ggcttgaggc gggcgatgcg cgatccggcg gcgtgcacgc ggaacggcgg atcgtcgccg
     4261 cgcgcctcgg gcgccggatc ccaagccaag ccgagcctgt gcatcaggtt gagggcgtcg
     4321 aacggcgcgc gcaccttcac gtcgttgtcg aaatagacgt acacgtcctt gccggcgcga
     4381 tcccaggcgc ggatgcgctt cgcccatcca tcgagcgccc ggggcgtata gccgctcttg
     4441 tagatctgca cgtcgccgtg caggcgcacg tagacgaaat ccgcggtgac gtcgaacatg
     4501 cgcgggaact tgcccgcggt gtcggccacc acgagcgcga tgtcgtgctc cctcagcagc
     4561 gaaacgaagg ccttgctgac gaagctcggg tggcggacct cgacggcgtg gcgcagcggg
     4621 cggtccgcgt cgatcgccaa ccgggacctt cccttgagac gggaatctct cttgcgcgcg
     4681 agcgcgagcg cgtcgcgcgt ggtcgtggga agcttttcga aaaaatccgc gagccggtcc
     4741 ggcttgaacg ggaacatcgg cgggaactgc cacaggaacg gcccgagctt ctccttcagc
     4801 gcgagcacgc ccgaggcgaa gaagttggcg agcggcttgt cgatctcctt cagccgccgc
     4861 aggtgcgtga tatagcgcga gcccttgacg gcgaagacga agcccggcgg ggcttgcgca
     4921 tgccagcgct cgtagtactc gggacgctgc agggaataga aggagccgtt gatctcgagc
     4981 gtcgggaagt ggcgcgcggc gaactccagc tcgcggcgct gcggcaggtc cttcggatag
     5041 aacacccctc gccacggctc gtaccgccag cccgagatgc cgatgcgaat caagcgaatc
     5101 tcagcttctt ccacgcctcc aggccgcgga ctttctcgag cttttcgtaa tcggtgagat
     5161 cgaggcgcag cggggtcatc gagacatagc cgtcttcgat cgcgcgccgg tccgaatcct
     5221 cctcggtgcc ttccaccggc accaccgtgt accagtagtg ggtgcgaccc atcggatcct
     5281 tggcagggac caccttgccg tcgtagtggc gcaccgactg gcaggtccag cgcgtgccgc
     5341 gcggcgcctt gtcggggaag ttgacgttga ccagggtgag ctcgggcgct tccagcagtt
     5401 gcttgagcac cgtcgccgcc caaggcttga gcagctcgaa gctcggcgcg tcgcggtcgg
     5461 tggccggcgc cgacagcgcg atgccgcgca ggccgagcag cgtcgcctga cgggcgccgg
     5521 cgagcgtgcc cgagtgccag atggcgttgc ccaggttggt gccgatgttg acgccggaga
     5581 ggaccaggtc gactttctcc cacaggctga cgccgagcgt gacgcagtcc gccggagtgc
     5641 cgttgacgcg gtaggcgtcg atgccattca gcggcgtgtg cttgtaggtc accggccgag
     5701 acgaagtgat ggagctgctc gccgaggaca tctcgacgtc gggcgccacc acgcgaactt
     5761 cgccgaacgc cgcggccacc tgagccaaag cggctatgcc cggactatag acgccgtcgt
     5821 cgttggtgat gaggatgcgc atatgactcc aatacagcaa ttaccgtgcg cgtcactctg
     5881 atccacaacc ccgccgccgg ccgggagggc gccggcgacg cccgcaagct ggagaagctc
     5941 ctgcgccgct tcggccacga cgtgcgctac cactcctcga aggacaaggg tttgcgccgc
     6001 attctcaaga aacgcgccga cctggtagcg gtcgccggcg gcgacgggac ggtgggaaag
     6061 gtcacacggc gcatggtgtc gcgcgatgtc ccgctcgcgg tgctgccttc gggaacggcc
     6121 aacaatgtcg cccgctcgct cggtctggtc gaccggccct tcgaggagtt ggtgcgcggg
     6181 tgggaccagg cgcggcgcgt caaactcgac gtcggcattg ccgccggtcc atggggcgag
     6241 cgctatttcg tggagggagt gggcgccggg ctgtttgccg atctcctcac gcgccaggga
     6301 agcgaccagg accagcgccg caagaagcgc aggggcaatc cggtcgagcg tgcgctgaag
     6361 cggctgaagg aagcggccga aagctgcgag ccggtggaca taagggcccg gctcgacggc
     6421 gaggacatat cgggcagcta tctgctcctc gaggctgtca acctgcgcta cgtcgggccg
     6481 aacctgcacc tggcgcacga cagccagccc ggcgacggcc agttcgacgt gatcatggcc
     6541 accgaggcgg agcgctcgcg cctggtgcac tacctcgagc attggcagga gaaccgcgag
     6601 cgcctcgcca tgctgccctc gcggcgcggc aagcggctgc agatcgaatg gaccggcttc
     6661 ccgctgcaca tcgacgacag gctggagccg aaggaggacg ccgagcccga cgagatggcg
     6721 ggcctggtgg aggcgcgcat tgcgcacgcg gtggagtttc tcgtgccggc gccgagccgc
     6781 taggcgcggc cctcagcggg cattgaagtt ggtgccgacc ttcgcccgct gaaagcttgc
     6841 cgctccgcgg cgtacgtccc agcttccttc cagtccgccg tcggtactga ggcaaagcac
     6901 gtggcggccc ggctccagcc ggaaccagat ttcctgctgc ttgcgcacgg tatcgtcgat
     6961 gcagcggcct tcgccgttga cgtggcccgc gctgaccgtt ccgctgcgca cgctgagcga
     7021 gacatcggtc agctcgcgag cgttcgcgca cagcagcatc cagccttcgc cgcgcgctgc
     7081 aatgggaacc gaggtgccgc tcgagccttc gcaatcggtg gccaggcgtg gggccggata
     7141 cctccgattt tcgtcgagaa agctggagaa atcccgctcc gagacgtcgt atagaaagat
     7201 cacgtgcttc atgtggcccg atgtctccag cttgtcgcgc gcccatttgt gctgtcgacg
     7261 cggtacccag agcacgccgc tcagctcgaa gctgtcgatc gcgttctttc cgggaagaac
     7321 gtgcaccgga ttgatgctcg cctgcacgcc gcgctccagc agcaacccgg ggatttgcgc
     7381 gtggtactcg ttccagtaga ggttggagtc ggcgagcacg cgatacgcat ccttcttcgg
     7441 cagcaggaag gagttgctga acgccagcgt gtggccgtag taaggaacca gctccgccag
     7501 ggaaaacacg gcgatggcgg cagcgacata ggcgcccctg ggtttgatcc accagtcctt
     7561 caatcccacc gcgaggaaca ggcaggtcat cggcaccagc atgaggacgt accggtatcc
     7621 cagctgggcg tggaagaaga aggaaaaata cagcagatag agcgcccact ggaagaacag
     7681 gagcttcatg ccgggctgac ggaacgcctg cgtgcgccat cccacccacg ctgccgcgac
     7741 cgacgcggcc atgatcgcga tcggcgtctt gagaaaccaa agaaccgcga agtagtacca
     7801 gacgccgtgc tcgaacaggc gatcgaagag gatgacgttg aacgactcgc gctcgtcggc
     7861 gaccaccttg tcgacgccga gcagaaacgg ctccggcagg ggaaggcgca gccagccgag
     7921 ccacgacgca agcaggcgga acggcgcggt gaggaacgga tactgggaca acggcttgcc
     7981 gaactcgcga aaataatacg
//
LOCUS       DASYJA010000556         6722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2808257, whole genome shotgun sequence.
ACCESSION   DASYJA010000556 DASYJA010000000
VERSION     DASYJA010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6722
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2808257"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1062
                     /locus_tag="VGO02_12110"
     CDS             91..1062
                     /locus_tag="VGO02_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEV7478469.1"
                     /translation="MPRAVRYHKQGGPEVLQLDEVTVGDPGQGQVRIRHTAIGVNFVD
                     TYQRSGLYPMQLPAVAGNEAAGVIDAVGPGVKDLKKGDRVAYTGLPGSYCDLRIVPAD
                     RMVKIPKGISDEQAASMMLKGMTVHYLIHSTYKVKKGDTVLWHAAAGGVGQIASQWLK
                     KLGVTVIGTVGSDEKATLAKAHGCKHVINYSTENFVEKVKEITKGKKLPVIYDAVGKT
                     TWEGSLDCLQPRGLMVSFGNASGPVAPVNLGILSTKGSLYVTRPTLATYIASRADLVK
                     RSNDLFNVVKSKKVKIETTARYKLADAAQAHRDLEGRKTTGSVILIP"
     gene            complement(1063..1302)
                     /locus_tag="VGO02_12115"
     CDS             complement(1063..1302)
                     /locus_tag="VGO02_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478470.1"
                     /translation="MKRITLAATLALFASVAFAHNCPNEMKAIDAALPNAKLTAAQSA
                     EVKQLRADGESLHKAGKHSESMAALGKAKGILGVK"
     gene            1318..1917
                     /locus_tag="VGO02_12120"
     CDS             1318..1917
                     /locus_tag="VGO02_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEV7478471.1"
                     /translation="MELDVAGLRQRLKRPLPESAVYGDDGTGRDAAATVAAVLVPIIA
                     HPAGLTVLFTQRTAHLKAHSGQVSFPGGRAEPGDASAEETALRESEEEIGLPRERVEV
                     LARLPEYFTRTGFRVTPVIGLIEPPLELRPDPGEVEAVFEVPLAFLLDARNHQRQTRE
                     FQGRSVGYYAMPYRTNEGDRTIWGATAGMVVNLSRALAD"
     gene            1928..2833
                     /locus_tag="VGO02_12125"
     CDS             1928..2833
                     /locus_tag="VGO02_12125"
                     /inference="COORDINATES: protein motif:HMM:NF005792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HEV7478472.1"
                     /translation="MGVLAIIAALLLEQWRPLGERKGWQAALSACIGWLEQTFNGGEE
                     RHGLVAWLVAVLPPVVLAVALNAALYAVHPFLALLFHGAVLYLTLGFRQFSHHFTQLQ
                     LAVKAGDIERARMLLDEVRGASGVVRTREEVIRLAIEEALLAAHRHVFGVIFWYMLLP
                     GPSGAILYRLAALLAARWRGLGAFGRFAQRAWRVLEWPAARLTAASFAVVGDFEDAVY
                     CWRTQARNWPEPEAGIVLAAGAGAMGVRLGMPVNEIEGVQPRVELGVGDPADGPFLDS
                     TVGLLWRALVVWVLVLAVLTVVRLL"
     gene            2830..3474
                     /locus_tag="VGO02_12130"
     CDS             2830..3474
                     /locus_tag="VGO02_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HEV7478473.1"
                     /translation="MSGRDRLLLALAAIVAVALVVSGIAPTDRITWVMEVVPVVIAGA
                     VIAATWQRFPLTTLLCILIAVHSLVLILGGAYTYAHVPLGFWLQDLLGTARNPYDKIG
                     HFMQGFVPALAAREIFVRGGYVTGRRMTAFLCICVALAVSAVYELIEWWAALAMGKGA
                     DEFLGTQGDQWDTQSDMFMALIGSCTAMALLARLHDRQIAALSSRDTPRSASTA"
     gene            complement(3434..3742)
                     /locus_tag="VGO02_12135"
     CDS             complement(3434..3742)
                     /locus_tag="VGO02_12135"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEV7478474.1"
                     /translation="MKRIYSSFNLAAVHHARNLLETEGIRAVVKNEFLSSAMGDLPPA
                     ECQAEVWILLETDAARAEACLKNAFSIKPSAPWKCPGCGEACEGQFTQCWRCGAYRES
                     "
     gene            complement(3753..4082)
                     /locus_tag="VGO02_12140"
     CDS             complement(3753..4082)
                     /locus_tag="VGO02_12140"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEV7478475.1"
                     /translation="MTELAKQKCKPCEGGVPPFNAQQTKEMLKQLKGWIVEDGKLVKV
                     YPFTNYYQTMAFVNALAWVSHREDHHPDLLVGYNKCRVEYSTHSIGGLSENDFICAAK
                     SDALFDL"
     gene            complement(4079..5323)
                     /locus_tag="VGO02_12145"
     CDS             complement(4079..5323)
                     /locus_tag="VGO02_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEV7478476.1"
                     /translation="MSNAFAWVFLAALAASSATRLWLAQRQMRHVRAHRGEVPQTFAA
                     AIPLDAHQKAADYTVAKARLGMLDVLIGAAVLLALTLGDGIQRLSELWAQVFPDGSIL
                     HGSALILSVVFLQSLVGLPLAVYRTFVVEQRFGFNRMTPRLFVTDLVKSTLLALALGV
                     PLLLAVLWLMERMGPWWWLYVWIVWTAFSLGLMMAYPAYILPLFNKFTPLAQGELSER
                     VERLLARTGFRSRGLYVMDGSKRSSHGNAFFAGLGAGKRIVLFDTLVSRLAPAEVEAV
                     LAHELGHYKLHHIAKALALSAAVSFAGLWLLGQLIDQPWFYQGLGVATPGTAAALLLF
                     MLVVPEFMFFLHPLTSRYSRKREFEADAYAMQHARSSDLVQALVKLYKDNAATLTPDP
                     LHSAFYDSHPPAATRIARLRTA"
     gene            5341..5886
                     /gene="orn"
                     /locus_tag="VGO02_12150"
     CDS             5341..5886
                     /gene="orn"
                     /locus_tag="VGO02_12150"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEV7478477.1"
                     /translation="MPADANNLIWIDMEMTGLNPDSDRIIEMAMLVTDPDLGLVAEGP
                     VLVLHQPDEVLEAMDSWNRGTHKKSGLIDKVRASRLAEAEAEAQAVAFLRQHVPANAS
                     PMCGNSICQDRRFLARWMPQLEAHFHYRNLDVSTLKELVRRWKPEAAKGFSKEGKHEA
                     LADILESIEELKYYRKTVMTI"
     gene            complement(5883..6386)
                     /locus_tag="VGO02_12155"
     CDS             complement(5883..6386)
                     /locus_tag="VGO02_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007739888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV7478478.1"
                     /translation="MSEKRLKELGIRLAPVSPPVANYVNAVRTGNLLYLAGKGGPPGP
                     DGKRPQGKLGREYTVEQGYQHARDTGLEILAVIRAELGSLDRVKRVVKVLGMVNAVPE
                     FADHPKVINGCSDLLVEVFGSRGKHARSAVGMGSLPMQIPVEIEVIVEAEPLRKKSSV
                     KAKRKRR"
     gene            complement(6388..>6722)
                     /locus_tag="VGO02_12160"
     CDS             complement(6388..>6722)
                     /locus_tag="VGO02_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478479.1"
                     /translation="RQGRPVMKTTLVVLVVPMLLLAAGAAAPQASAPATPPTAQPPER
                     PAAPPRLILRLDEIEGPRMSIGRSAGETEAKKDLPALGGDARPVDLSKPRESIFPKES
                     TSNIPTPY"
BASE COUNT         1077 a         2204 c         2352 g         1089 t
ORIGIN      
        1 ctggtaggat tcttctgcaa cggcgagatc tgtcacgacc ggctgtacgg gtacaccggc
       61 gttctgacgc tatttcatta ggagcattcc atgccaaggg cagttcgata ccacaagcag
      121 ggcgggccgg aagtgttgca gctcgacgaa gtgaccgtcg gcgatcccgg ccagggccag
      181 gtgcgcatcc gccacaccgc gatcggcgtc aatttcgtcg acacctacca gcgctcgggc
      241 ctgtacccga tgcagctccc cgccgtggcg gggaacgagg ccgccggcgt gatcgatgcc
      301 gtggggccgg gcgtcaagga cctgaagaag ggcgaccggg tcgcctatac cgggctgccc
      361 gggtcgtact gcgacctgcg catcgtgccc gccgaccgta tggtgaagat ccccaagggc
      421 atcagcgacg agcaggcggc gtcgatgatg ctgaagggca tgacggtgca ctacctcatc
      481 cactccacct acaaggttaa gaaaggggac accgtgctat ggcatgccgc cgccggcggc
      541 gtcggccaga tcgccagcca gtggctgaag aagctcggcg tcaccgtcat cggcaccgtc
      601 ggctccgacg aaaaagcgac gctggcgaag gcgcacggct gcaagcacgt catcaactac
      661 agcaccgaga acttcgtcga gaaggtgaag gagatcacca agggcaagaa gctgccggtg
      721 atctacgacg cggtgggcaa gacgacctgg gaaggctcgc tcgactgcct gcagccgcgc
      781 ggcctgatgg tgtccttcgg caacgcctcg ggaccggtgg cgccggtcaa cctcggcatc
      841 ctgtcgacca agggctcgct ctacgtcacg cggccgaccc tcgccaccta catcgcgagc
      901 cgcgccgacc tggtcaagcg ctcgaacgac ctgttcaacg tggtcaagtc gaagaaggtc
      961 aagatcgaga ccacggcgcg ctacaagctg gcggacgccg cgcaggcgca ccgcgatctc
     1021 gaggggcgca agaccaccgg ctcggtgatc ttgatcccgt gattacttga cgcccaggat
     1081 gcccttggcc ttgccgaggg cagccatcga ctcggagtgc ttgccggcct tgtgaagcga
     1141 ctcgccatcg gcacgcagct gcttcacctc ggcgctttgc gcggcggtga gcttggcgtt
     1201 gggcagggcg gcatcgatcg ccttcatctc gttcgggcag ttgtgggcga aggctacgct
     1261 ggcgaacagg gcgagggtgg cggctagggt aatgcgcttc atgcagtgtc tccgttgatg
     1321 gaactcgatg ttgccggatt gaggcagcgc ctcaagcgcc ccttgccgga atccgcagta
     1381 tacggcgacg acggcaccgg gcgcgacgcg gcggccaccg tggcggcggt gctggtgccg
     1441 atcattgccc accccgccgg cctgaccgtg ctttttacgc agcgcacagc gcacctcaag
     1501 gcgcattcgg ggcaggtcag cttccctggc ggccgggccg agcccggcga cgcgtcggcg
     1561 gaggagacgg cgctgcgcga gtcggaggag gagatcggcc tgccgcgcga gcgcgtcgag
     1621 gtgctggcga gattgccgga atatttcacg cgcaccggct ttcgcgtgac gccggtgatc
     1681 gggctgatcg agccgccgct cgagctcagg cccgacccgg gcgaggtcga ggcggtgttc
     1741 gaggtgccgc tcgcctttct cctcgacgcg cgcaaccacc agcgccagac gcgcgaattt
     1801 cagggccgga gcgtcggcta ctacgccatg ccctatagga caaatgaggg cgacagaacc
     1861 atctggggcg ccaccgccgg aatggtggtc aatctctccc gggcgctggc cgactagaat
     1921 cgggctaatg ggggtcctcg ccatcatcgc cgcgctgctc ctcgagcagt ggcggccgct
     1981 cggcgagcgc aagggctggc aggcggcgct cagcgcctgc atcggctggc tcgagcagac
     2041 cttcaacggc ggtgaggagc gccacggtct ggtcgcctgg ctggtcgcgg tgctgccgcc
     2101 ggtcgtcctg gcggtcgcgc tgaacgcggc cctctacgcg gtgcacccgt tcctcgcgct
     2161 tctgttccac ggcgcggtgc tctatctgac gctcggcttc cgccagttca gccaccactt
     2221 cacccagctg cagctcgcgg tcaaggcggg cgacatcgag cgcgcacgca tgctgctcga
     2281 cgaggtgcgc ggcgcctcgg gcgtggtgcg cacgcgcgag gaggtgatcc gcctcgccat
     2341 cgaggaggcg ctgctcgccg cgcatcgcca cgtcttcggc gtgattttct ggtacatgct
     2401 gctgcccggg ccgagcggcg cgatcctgta ccgcctggcc gcgctgctcg ccgcgcgctg
     2461 gcgcggcctg ggcgccttcg gccgcttcgc gcagcgcgcc tggcgcgtgc tggagtggcc
     2521 ggcggcgcgc ctgactgccg cctcgttcgc ggtggtcggc gacttcgagg acgcggtcta
     2581 ctgctggcgc acgcaggcgc gcaactggcc cgagccggaa gccggcatcg tgctcgccgc
     2641 cggagccggc gcgatgggcg tgcgcctggg catgccggtg aacgaaatcg agggcgtgca
     2701 gccgcgcgtc gagctcggcg tcggcgatcc ggccgacggg ccgttcctcg acagcacggt
     2761 cggcctgctg tggcgggcgc tggtcgtctg ggtactggtg ctggcggtgc ttacggtggt
     2821 gcggctactt tgagcggccg cgaccgcctc ctgctggcgc tcgccgccat cgtcgcggtg
     2881 gcgctggtcg tttcgggcat cgcgcccacc gaccgcatca cctgggtgat ggaggtagtg
     2941 cctgtcgtca tcgccggcgc ggtcatcgcc gcgacctggc agcgatttcc gctgaccacg
     3001 ctgctctgca tcctgatcgc cgtgcactcg ctcgtgctga ttctcggcgg cgcctacacc
     3061 tacgcccacg tgccgcttgg cttctggctg caggacctgc tcggcacggc gcgcaacccc
     3121 tacgacaaga tcggccactt catgcagggc ttcgtgccgg cgctggcggc gcgcgagatc
     3181 ttcgtgcgcg gcggctacgt cacgggccgg cgcatgacgg ctttcctgtg catctgcgtc
     3241 gcgctcgcgg tgagcgcggt ctacgagctg atcgagtggt gggccgcgct cgccatggga
     3301 aagggggccg acgagttcct cggcacgcag ggcgaccagt gggacacgca atccgacatg
     3361 ttcatggcgc tgatcggctc gtgcaccgcc atggccctgc tcgcgcgcct gcacgaccgg
     3421 cagatcgcgg cactcagctc tcgcgatacg ccccgcagcg ccagcactgc gtgaactggc
     3481 cttcgcaggc ctcgccacac cccgggcatt tccacggcgc gctgggtttg atcgaaaacg
     3541 cgttcttcag acaagcctcg gcgcgagcag catccgtttc gagaaggatc cagacctccg
     3601 cctgacattc cgccggcggc agatcgccca tcgccgaaga gaggaattcg ttcttgacga
     3661 cagcgcggat gccctcggtc tcgaggaggt tgcgcgcgtg atgcaccgcg gcgagattga
     3721 acgagctgta gatgcgcttc aaagatcccg catcacaagt cgaacaaggc gtcggacttg
     3781 gccgcgcaga tgaagtcgtt ctcggacagg ccgccgatgg aatgcgtcga gtactcgacg
     3841 cggcacttgt tgtagccgac cagcaggtcg gggtggtggt cctcgcggtg cgagacccag
     3901 gcgagcgcgt tgacgaaggc catggtctgg tagtagttgg tgaacgggta caccttcacc
     3961 agcttgccgt cctccacgat ccagcctttc agctgcttca gcatctcctt tgtctgctgg
     4021 gcgttgaagg gcggcacgcc gccttcgcag ggtttgcact tttgctttgc aagttccgtc
     4081 atgctgtcct cagtcgcgcg atgcgcgtgg cggcgggcgg gtgggagtcg tagaaggcgg
     4141 aatgcagcgg gtccggcgtc agggtggccg cgttgtcctt gtagagcttg accagcgctt
     4201 gcaccagatc ggacgacctg gcgtgctgca tggcataggc gtcggcctcg aactcgcgct
     4261 tgcgcgaata gcggctggtg agcggatgca ggaagaacat gaactcgggc accaccagca
     4321 tgaacaggag cagcgccgcg gcggtgcccg gggtggcgac gcccaggccc tggtagaacc
     4381 acggctggtc gatgagctgg ccgagcagcc agaggccggc gaagctgacc gcggcgctga
     4441 gcgcgagcgc cttcgcgatg tggtgcagct tgtagtgccc gagctcgtgc gccagcaccg
     4501 cctcgacttc cgccggggcg aggcggctca ccagcgtgtc gaacagcacg atgcgcttgc
     4561 ccgcgccgag gccggcgaag aaggcgttgc cgtggctcga gcgcttcgag ccgtccatca
     4621 cgtagaggcc gcgcgagcgg aagccggtgc gcgcgaggag gcgctcgaca cgctcggaca
     4681 gctcgccctg agcgagcggc gtgaacttgt tgaacagcgg caggatgtag gccggatacg
     4741 ccatcatcag gccgagcgag aacgcggtcc acacgatcca cacgtacagc caccaccagg
     4801 gtcccatgcg ctccatcagc cacagcacgg cgaggagcag cggcacgccg agcgccaggg
     4861 cgagcagcgt gctcttcacc aggtcggtga cgaacaggcg cggcgtcatg cggttgaagc
     4921 cgaagcgctg ctcaacgacg aaggtgcggt agacggcgag cggcaggccg accagcgact
     4981 gcaggaagac gacgctcagg atgagcgcgc tgccgtgcag gatcgagccg tcggggaaga
     5041 cctgtgccca cagctccgac aggcgttgga tgccgtcgcc gagggtgagc gcgagcagca
     5101 ccgcggcgcc gatcagcacg tcgagcatgc ccaggcgcgc cttggccacg gtgtagtcgg
     5161 cggccttctg gtgcgcgtcg agcgggatcg ccgcggcgaa agtctgcggc acctcgccgc
     5221 ggtgcgcgcg cacgtggcgc atctggcgct gcgcgagcca cagccgcgtc gcgctcgagg
     5281 cggcgagcgc ggcgaggaac acccaggcga acgcgttgct catgcgtgag agaataaagc
     5341 atgcctgccg atgccaacaa cctcatctgg atcgacatgg agatgaccgg gctcaacccc
     5401 gacagcgatc gcatcatcga gatggcgatg ctggtcaccg atcccgacct cggcctggtg
     5461 gccgaggggc cggtgctggt gctgcaccag cccgacgagg tgctcgaggc gatggattcc
     5521 tggaacaggg gcacgcacaa gaagtcgggc ctgatcgaca aggtgcgcgc ctcgcgcctg
     5581 gccgaggccg aggccgaggc gcaggcggtg gcgttcctgc gccagcacgt gccggcgaac
     5641 gcctcgccga tgtgcggcaa ctcgatctgc caggaccggc gcttcctcgc gcgctggatg
     5701 ccgcagctcg aggcgcattt ccactaccgc aacctcgacg tctcgacgct caaggagctg
     5761 gtgcggcgct ggaagccgga ggcggcgaaa ggattctcca aggaaggcaa gcacgaggcg
     5821 ctcgccgaca tcctcgagtc gatcgaggag ctcaagtact accgcaagac ggtgatgacg
     5881 atctagcggc gcttccgttt cgccttcacg gaagatttct tccgtaaagg ctcggcttca
     5941 acgatcacct cgatctcgac cgggatctgc atcggcagcg agcccatgcc gaccgccgag
     6001 cgcgcgtgct tgccgcggct gccgaacacc tcgaccagaa ggtcggagca gccgttgatc
     6061 accttcgggt ggtcggcgaa ctcgggcacg gcgttcacca tgccgagcac cttcaccacg
     6121 cgcttcacgc ggtccaggct gcccagctcg gcgcggatca cggcgaggat ctccaggccg
     6181 gtgtcgcgtg cgtgctggta gccctgctcg acggtgtatt cgcggccgag cttgccctgc
     6241 ggccgcttgc cgtcggggcc cggcgggccg cccttgccgg cgaggtacag caggttgccg
     6301 gtgcgcacgg cattgacgta gttcgcgacc ggcggcgaca ccggcgcgag ccggatgccg
     6361 agctccttca gccgtttctc gctcatccta gtagggcgtg ggaatgttgc tggtggattc
     6421 cttcgggaag atcgactcgc gcggtttcga caggtccacc ggacgggcgt ctccgcccag
     6481 agccgggaga tccttcttgg cctccgtttc gcctgcggaa cgaccgatgc tcattctcgg
     6541 cccttcgatc tcgtcgaggc gcagaataag gcgggggggc gccgcaggac gctccggcgg
     6601 ttgcgccgtg ggcggcgtcg cgggcgcgga cgcctgcggc gcagccgcgc cggcggcgag
     6661 caggagcatc gggacaacga gcactaccaa ggttgttttc ataacgggac gaccttgccg
     6721 gg
//
LOCUS       DASYJA010000557         4268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2718144, whole genome shotgun sequence.
ACCESSION   DASYJA010000557 DASYJA010000000
VERSION     DASYJA010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2718144"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VGO02_12165"
     CDS             <1..222
                     /locus_tag="VGO02_12165"
                     /inference="COORDINATES: protein motif:HMM:NF020331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEV7478480.1"
                     /translation="RIFAVGDGSGKWTERAGEWQPIQSRTGLGWPSVLARQFFCPHRD
                     PIQSVAEGVDALRRGAHPAVYVEQRNQGL"
     gene            complement(230..1342)
                     /gene="proB"
                     /locus_tag="VGO02_12170"
     CDS             complement(230..1342)
                     /gene="proB"
                     /locus_tag="VGO02_12170"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEV7478481.1"
                     /translation="MSLKEARTLVVKVGSSLVTNQGRGLDTAAIARWAGEVAELRKAG
                     RGVLLVSSGAIAEGMQRLGWAKRPHTMHELQAAAAVGQMGLVQCYESCFREHGLRTAQ
                     VLLTHADMADRQRYLNARSTLRTLLALGVIPVINENDTVVTDEIKLGDNDTLGALVTN
                     LVEADALVILTDQAGLYDADPRKNPQAKFIESIEANDPALEAMAGGVGSALAKGGMLT
                     KVLAAKRAARSGAQTVIASGREPGVLVRLARGESVGTLLTVQTVPLAARKQWLADHLT
                     VNGQVRLDAGAVKALARDGKSLLPIGVVEVTGDFERGAVVGVLDAAGREVARGLINYS
                     SEETRRIMRRPSGEIEAVLGYVDEPELIHRDNLVLL"
     gene            complement(1369..2346)
                     /gene="obgE"
                     /locus_tag="VGO02_12175"
                     /pseudo
     CDS             complement(1369..2346)
                     /gene="obgE"
                     /locus_tag="VGO02_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
     gene            complement(2411..2668)
                     /gene="rpmA"
                     /locus_tag="VGO02_12180"
     CDS             complement(2411..2668)
                     /gene="rpmA"
                     /locus_tag="VGO02_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEV7478482.1"
                     /translation="MAHKKAGGSSRNGRDSKSKRLGVKVYGGERVNAGQILVRQRGTV
                     VHPGDNVGMGRDHTLFARVAGQVNYAVKGESSRKTVNVVPA"
     gene            complement(2672..2986)
                     /gene="rplU"
                     /locus_tag="VGO02_12185"
     CDS             complement(2672..2986)
                     /gene="rplU"
                     /locus_tag="VGO02_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEV7478483.1"
                     /translation="MYAVIKSGGKQYRVQSGEQVRVESLPGDVGSAVSFEEVLLVGSG
                     DAVKVGAPLVSGAKVKATVVSHGRGEKVKIFKLRRRKHYQKTQGHRQNFTEVRIDDIV
                     QG"
     gene            3032..4012
                     /locus_tag="VGO02_12190"
     CDS             3032..4012
                     /locus_tag="VGO02_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV7478484.1"
                     /translation="MSFLSMLAPVAEDVERVDALIRRRLDSDVALVSQVAEYIVASGG
                     KRLRPALLLLACGAAGFREKPGGEVRLQLAAVVEFIHTATLLHDDVVDESPLRRGRST
                     ANAAFGNAAAVLVGDFLYSRAFQMMVEADNMRVMQVLAEATNTIAGGEVLQLMGSHDP
                     AVDEARYLEVIRRKTAKLFEAAARLGGVLAGSPAAIEAGLADYGMHVGTAFQLIDDVL
                     DYSGDAATIGKSLGDDLAEGKPTLPLIRVMRTGSAEQAALVRRAIVEGGREAFAGVLE
                     AIRACGALDYAREAARREAEAAARAIAPLPASEFKQSLLELASFSVTRSS"
     gene            4027..4103
                     /locus_tag="VGO02_12195"
     tRNA            4027..4103
                     /locus_tag="VGO02_12195"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4061..4063,aa:Pro,seq:cgg)
BASE COUNT          586 a         1509 c         1465 g          708 t
ORIGIN      
        1 cgcatctttg ccgtcggcga cggcagcggc aagtggaccg agcgcgcggg cgaatggcag
       61 ccgatccagt cgcgcaccgg gctgggctgg ccttcggtgc tggcgcggca gttcttctgc
      121 ccgcaccgcg acccgatcca gagcgtggcc gagggcgtcg atgcgctaag acgcggcgcg
      181 catcccgcgg tttacgtcga gcagcggaac cagggcctgt aaccccgcgc tagagcaata
      241 ccaggttgtc ccggtggatg agctcgggct cgtccacgta gccgagcacg gcctcgattt
      301 cgcccgacgg gcggcgcatg atgcggcgcg tctcctcgga ggagtagttg atcaggccgc
      361 gcgccacttc gcggccggcg gcgtcgagca ccccgaccac cgcgccgcgc tcgaagtcgc
      421 cggttacttc cactacgccg atcggcagca ggctcttgcc gtcgcgcgcg agcgccttca
      481 ccgcgcccgc gtcgagcctg acctggccgt tgacggtgag gtggtcggcg agccactgct
      541 tgcgggccgc gagcgggacg gtctgcacgg tgagcagcgt gccgaccgac tcgccgcggg
      601 cgaggcgcac cagcaccccg ggctcgcggc ccgaggcgat gacggtctgc gcgccgctgc
      661 gcgcggcgcg ctttgccgcc aggaccttgg tgagcatgcc gcccttcgcc agcgccgagc
      721 cgaccccacc ggccatcgcc tccagcgccg ggtcgttcgc ctcgatcgat tcgatgaact
      781 tcgcctgcgg gttcttgcgc ggatccgcgt cgtacaggcc ggcctggtcg gtgaggatca
      841 ccagggcgtc ggcctcgacc aggttggtga ccagcgcgcc gagcgtgtcg ttgtcgccga
      901 gcttgatctc gtcggtcacc acggtgtcgt tctcgttgat caccgggatc acgccgagcg
      961 cgagcagcgt gcgcagcgtc gagcgcgcgt tcaggtagcg ctggcggtcg gccatgtcgg
     1021 cgtgcgtcag gagcacctgc gccgtgcgca ggccgtgctc gcggaagcac gactcgtagc
     1081 attgcaccag gcccatctgt cccaccgccg ccgccgcctg cagctcgtgc atcgtgtgcg
     1141 gccgcttcgc ccagcccagg cgctgcatgc cctcggcgat ggcgccgctc gacaccagca
     1201 ggacgccgcg tcccgccttg cgcagctcgg ccacctcgcc ggcccagcgc gcgatggcgg
     1261 cggtgtccag cccgcggccc tggttggtga ccagcgacga gccgaccttc accaccaggg
     1321 tgcgcgcctc cttcaggctc atgccgccac cgccgagaat tcgcgcgcga tcgctttcac
     1381 gaccacatcg cagcccttgc cgctgatggc ggcgaccttg aaccaggggc gcttccagcg
     1441 caacgcccgc acgatgcggg caatcctttc gtctgcgtcg ggtgcgaggt cggccttgtt
     1501 gaacaccagc cagcgcggcc ggcgcgcaag ctggtcgtcg tatttcttca gctctgcggc
     1561 aattgccttg gcgtcgcgca ccgggtcggc ctccgggtcg aaaggggcga tgtcgatgat
     1621 atgcagaaga agccgggtgc gtgcgagatg gcgcaggaac tggtggccga gcccggcgcc
     1681 ctcggcggcg ccttcgatca ggcccgggat gtcggccacc acgaagctgg tctcgcccac
     1741 gcgcaccatg cccagcgacg gcgcgagggt ggtgaacggg tagtcggcca ccttgggccg
     1801 tgcggccgac acggcgcgga tgaaggtcga cttgcccgcg ttcggcaggc cgagcaggcc
     1861 gacgtcggcg agcacgcgca gctcgagcgt gagcttgcgg ccctcgccgg gctcgccccg
     1921 cgtgaactgg cgcggcgcgc ggttgatgct cgagcggaag tgcaggttgc ccaggccgcc
     1981 cttgccgccg cgggcgagca gcatgcgctc cccgtccttg gcgaggtcgg cgaccagctc
     2041 gccgctttcc gcgtcgcgga ttacggtacc caccggcacg cgcagcacga cgtcctcgcc
     2101 cgagcgtcca ttgcaatccg cgcccatgcc gcgcttgccg tgatcggcgc gatgcaggcg
     2161 cgcgaagtgg tagtcgacca gcgtgttgac gttgcggtcg gcgtgcgccc agacgctgcc
     2221 gccgcgcccg ccgtcgccgc cgtccgggcc gcctttctcc acgtacttct cgcggcgaaa
     2281 gctggccgag ccgtcgccac ccttgccggc gtgcacctcg atcttcgctt cgtcgatgaa
     2341 tttcatgggc ccggaaatga aaaggcccta tcggttctcg atagggccct cgttgtcgcc
     2401 agggactggc ttaagccggt actacgttga ccgtcttgcg cgagctctcg cctttgaccg
     2461 cgtagttgac ctggcccgcg acgcgcgcga acagcgtgtg atcgcggccc atgccgacgt
     2521 tgtccccggg atgaaccacc gtgccgcgct ggcgcaccag gatctggccg gcgttgacgc
     2581 gctcgccgcc gtagaccttg acgccgaggc gcttggactt cgagtcgcgc ccgttgcgtg
     2641 aactgccgcc tgccttttta tgtgccatgc gctatccctg cacgatgtcg tcgatgcgga
     2701 cctcggtgaa gttctgccga tggccctgcg ttttctggta gtgcttgcgg cggcgcagct
     2761 tgaagatctt caccttctcg ccgcggccgt gcgacacgac ggtggctttc accttggcac
     2821 cggaaaccag cggcgcgccg accttcaccg cgtcgcccga gccgaccagc agcacttcct
     2881 cgaaggaaac cgcgctgccg acgtcgcccg gaagggattc gacgcgcacc tgttcgcctg
     2941 attgcaccct gtactgcttg ccgccggact tgatcactgc gtacatggca attgctctta
     3001 caatcgaaag gggcggaatt ataaaggaat catgagtttc ctgtctatgc tggcgccggt
     3061 cgcggaggac gtggagcggg tcgatgcgct catccgccgg cgcctcgact ccgacgtcgc
     3121 cctggtgagc caggtggccg agtacatcgt cgcctcgggc ggcaagcggc tgcgcccggc
     3181 gctcctcctg ctcgcctgcg gcgcggccgg cttccgcgaa aagccgggcg gcgaagtgcg
     3241 cctgcagctc gcggcggtgg tcgagttcat ccacaccgcc accctgctgc acgacgacgt
     3301 ggtcgacgag tcgccgctgc gccgcgggcg cagcaccgcc aacgcagcgt tcggcaacgc
     3361 cgccgcggtg ctggtcgggg acttcctcta ctcgcgcgcc ttccagatga tggtcgaggc
     3421 cgacaacatg cgcgtgatgc aggtgctggc cgaggccacc aacaccatcg ccggcggcga
     3481 agtgctgcag ctgatgggca gccacgaccc ggcggtcgac gaggcgcgct acctggaggt
     3541 gatccggcgc aagacggcga aattgttcga ggccgcagcg cgcctgggag gcgtgctcgc
     3601 cggctcgccc gccgcgatcg aggccggcct cgccgactac ggcatgcacg tcggcaccgc
     3661 cttccagctg atcgacgacg tgctcgacta ttcaggcgac gccgcgacca tcggcaagag
     3721 cctcggcgac gacctggccg agggcaagcc gacgctgccg ctgatccgcg tcatgcgcac
     3781 cggcagcgcc gagcaggcgg cgctcgtgcg ccgcgccatc gtagagggcg gccgcgaggc
     3841 gttcgccggg gtgctggagg cgatccgcgc ctgcggcgcg ctcgactacg cgcgcgaggc
     3901 ggcgcgccgc gaagccgagg cggcggcgcg cgccatcgcg ccgcttcccg cctcggaatt
     3961 caagcaatct ttgctagaat tggcgtcctt ttcggtgacc cgcagcagct agattttcgc
     4021 ctcggtcggg gcgtagctca gcctggtaga gtactgcgtt cgggacgcag gtgtcgctgg
     4081 ttcaaatcca gtcgccccga ccactcacct tcaactccct gcaggcccgt ctttcttggt
     4141 cgtgtccccc ggttcgggcg gcgacgctgt gcgcggcgcg tcttgcgccg gtttcgccgc
     4201 gtggcgctcc agcagcgcga ccagcgcgtt ggggtcgtat ggcttggtca gatgatggtc
     4261 gaacccgg
//
LOCUS       DASYJA010000558         2683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1902460, whole genome shotgun sequence.
ACCESSION   DASYJA010000558 DASYJA010000000
VERSION     DASYJA010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1902460"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VGO02_12200"
     CDS             <1..607
                     /locus_tag="VGO02_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV7478485.1"
                     /translation="EERRCERPLNLYAYSKFLFDQVVRQMLQARTAQVAGLRYFNVYG
                     PNEQHKVYGSDMRSVAYQAFHQFKAEGKVKLFVGSGGYADGEQRRDFIHVDDVAAVNM
                     WLLERRDVSGIFNCGSGRAQTFNDVASSVINSVKGTQSSAAELASQGLITYIPFPPQL
                     VGKYQSFTQADLSRLRAAGLPGEFMSVEQGVAAYVAELSKK"
     gene            604..1500
                     /gene="cysM"
                     /locus_tag="VGO02_12205"
     CDS             604..1500
                     /gene="cysM"
                     /locus_tag="VGO02_12205"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HEV7478486.1"
                     /translation="MKKTIEDFIGETPLVRIQRLDSALQNNKNVILAKLEGNNPAGSV
                     KDRPAMSMIRHAEKRGTIKPGDTLIEPTSGNTGIALAMAAAIRGYRMILLMPEHLSAE
                     RRQTMRAFGAQIILTPQKGGMEAARDLADKMVREGKGTMLDQFANPDNPLIHYETTGP
                     EIWRDTEGRISHLVSSMGTTGTIMGCSRFLKEKNPAIQIIGCQPEEGSQIPGIRKWPE
                     AYLPKIYDRKRVDRIESVSQGEAEEMTRRMAREEGIFAGISSGGAMHVALRLSKELKN
                     AVIVTIVCDRGDRYLTTGVFPD"
     gene            1500..2270
                     /locus_tag="VGO02_12210"
     CDS             1500..2270
                     /locus_tag="VGO02_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV7478487.1"
                     /translation="MTPVLAFDIETVPDIAGIRRLHRLPEDLPDAEVAEVAFQKRRIQ
                     TGSDFLGPHLHRVLIISCVLREGDSVKVFSLGEATEKELIQRFYDGIERLVPQLVSWN
                     GGGFDLPVLNYRAMANGVAAPKFWDQGEDDKDFRYNSYLGRYHARHLDLMDVLAMYQP
                     RSSAPLDEVAQLAGFPGKLGVGGAQVWNAYQAGKIGEIRNYCEADTVNTYLLYLRFQQ
                     LRGALTAEQYGRECGLLRGVLEKRTEPHWREFLSLWKT"
     gene            2336..>2683
                     /locus_tag="VGO02_12215"
     CDS             2336..>2683
                     /locus_tag="VGO02_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase"
                     /protein_id="HEV7478488.1"
                     /translation="MFVEGALPREQVSAQVVNRKSKYEFLKATAIIHESSGRRAPRCP
                     HFGVCGGCATQHADARTQMAAKQRALEENLARIGKVQPETMLPIVYGEEWGYRRRARW
                     SVRHVPGKGGAMVG"
BASE COUNT          491 a          897 c          895 g          400 t
ORIGIN      
        1 cgaggagcgg cgctgcgagc gaccgctcaa tctatacgcg tactcgaagt tcctcttcga
       61 ccaggtcgtg cggcagatgc tccaggcgcg cacggcgcag gtggccgggc tgcgctactt
      121 caatgtgtac ggacccaacg agcaacataa ggtgtatggc tcggatatgc gctcggtggc
      181 ctaccaggcg ttccatcagt tcaaggctga aggcaaggtg aagctgtttg taggcagcgg
      241 cggctatgcc gacggcgagc agcggcgcga cttcatccac gtcgacgacg tcgccgcggt
      301 gaacatgtgg ctgctcgagc ggcgcgacgt gtccggcatc ttcaactgcg gcagcggccg
      361 ggcgcagacc ttcaacgacg tcgccagctc ggtgatcaac tccgtgaagg gcacccaatc
      421 ctccgccgcg gagctggcga gccaggggct gatcacctac atcccgttcc cgccgcagct
      481 cgtcggcaag taccagagct tcacccaggc ggatctgtcg cggctgcgcg ccgccgggct
      541 gccgggcgag ttcatgagcg tcgagcaggg cgtcgccgcc tacgtcgcgg agctgtcgaa
      601 gaaatgaaaa agaccatcga ggacttcatc ggcgagacgc cgctggtgcg catccagcgt
      661 ttggattctg ctttgcaaaa caataagaat gtcattcttg cgaagctgga gggcaacaat
      721 cccgccggct cggtgaagga ccggccggcg atgtcgatga tccgccacgc cgagaagcgc
      781 ggaaccataa aacctgggga tacgctgatc gagccgacct ccggcaacac cggtatcgcg
      841 ctcgccatgg cggcggcgat ccgcggctac cggatgatcc tgctgatgcc cgagcacctc
      901 tcggccgagc ggcgccagac catgcgcgcc ttcggcgcgc agatcatcct cacgccgcag
      961 aagggcggca tggaggcggc gcgcgacctg gccgacaaga tggtgcgcga gggcaagggc
     1021 acgatgctcg accagttcgc caatcccgac aatccgctga tccactacga gaccaccggg
     1081 ccggaaatct ggcgcgatac cgagggcagg atcagccacc tggtctccag catgggcacc
     1141 accggcacca tcatgggctg ctcgaggttc ctcaaggaga agaacccggc catccagatc
     1201 atcggctgcc agcccgagga aggctcgcag atccccggca tccgcaagtg gcccgaggcc
     1261 tacctgccga agatctacga ccgcaagcgc gtcgaccgca tcgagtcggt gagccagggc
     1321 gaggcggagg agatgacgcg gcgcatggcg cgcgaggaag gcattttcgc cggcatctcc
     1381 tccggcggcg cgatgcacgt ggcgttgcga ctttcgaagg agctcaagaa cgcggtgatc
     1441 gtcacgatcg tctgcgatcg cggcgaccgc tacctgacga ccggcgtctt tccggactga
     1501 tgaccccggt cctggcgttc gatatcgaga cggtgcccga catcgccggc atccggcggc
     1561 tgcaccggct gcccgaggac ctgccagacg ccgaggtcgc cgaagtcgcc ttccagaagc
     1621 gccgcatcca gaccggcagc gacttcctcg gcccgcacct gcaccgcgtg ctcatcatct
     1681 cctgcgtgct gcgcgagggc gacagcgtca aggtgttctc gctcggcgag gcgaccgaga
     1741 aggagctgat ccagcgcttc tacgacggca tcgagcggct ggtgccgcag ctcgtctcgt
     1801 ggaacggcgg cggcttcgac ctgccggtgc tcaactaccg cgccatggcc aacggcgtcg
     1861 ccgcgccgaa gttctgggac cagggcgagg acgacaagga cttccgctac aacagctacc
     1921 tcggccgtta ccacgcgcgc catctcgacc tgatggacgt gctggcgatg taccagccgc
     1981 gctcgagcgc gccgctcgac gaggtggcgc agctcgccgg cttccccggc aagctcggcg
     2041 tcggcggcgc ccaggtgtgg aacgcctacc aggcgggaaa gatcggcgag atccgcaact
     2101 actgcgaggc cgacaccgtc aacacgtatc tgctctacct gcgcttccag cagctgcgcg
     2161 gtgccctcac cgccgagcag tacggccggg agtgcggcct gctgcgcggc gtcctcgaga
     2221 agcgcaccga gccgcactgg cgcgagttcc tgtcgctctg gaaaacctga tcctcgacat
     2281 cgagtcgctg gacgccgagg gccgcggcgt cgcgcacaat gccgaaggca aggtgatgtt
     2341 cgtcgagggc gcgctgccgc gcgagcaggt ctcggcccag gtagtcaacc gcaagtcgaa
     2401 atacgagttt ctgaaagcaa ccgccatcat tcatgaatcg tcgggacggc gggcgccgcg
     2461 ctgcccgcac ttcggcgtct gcggcggctg cgccacgcag cacgcggacg cgcgcacgca
     2521 aatggccgcc aagcagcgcg cgctggagga gaacctggcg cgcatcggca aggtccagcc
     2581 cgagacgatg ctgcccatcg tctacggcga agagtggggc taccgccgcc gcgcgcgctg
     2641 gtcggtgcgc cacgtgccgg gcaagggcgg cgcgatggtc ggc
//
LOCUS       DASYJA010000559         3784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2718506, whole genome shotgun sequence.
ACCESSION   DASYJA010000559 DASYJA010000000
VERSION     DASYJA010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2718506"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VGO02_12220"
     CDS             <1..745
                     /locus_tag="VGO02_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEV7478489.1"
                     /translation="LAQLLRSAYSRNLLDADAMSIIEGALTVSEMQVRDIMIPRAQVD
                     FIDINEPVESFIPQVMATAHSRFPVIDQNKDDVIGVLLAKDLLRHYAGEEEFNVREML
                     RPAVFVPEAKRLNVLLREFRASRNHMAIVIDEYGGVAGLVTIEDVLEQIVGDIEDEYD
                     FDEAADNILPEAGGRWRVKALTQIADFNAHFGTHFPDEASDTVGGLVIKHLGRLPRRG
                     EVHIIEGLRFQVLRADSRRVYTLLVDKKT"
     gene            742..2190
                     /gene="lnt"
                     /locus_tag="VGO02_12225"
     CDS             742..2190
                     /gene="lnt"
                     /locus_tag="VGO02_12225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEV7478490.1"
                     /translation="MTAFLAGAAAVPAFAPIGFFPIALLAFAWLAHLWANASPRRAFW
                     TGFAFGMGLFGAGVSWVYVSLSQFGGMPPPLAALATFLFCALLAAFTGGAGWLQARVP
                     ASEPVRAALLIPASWTLFEWLRSWVFTGFPWLAAGYATTGWPLQGFAPLLGVFGASFL
                     VVSLAGMLCWFARRRRALALAPIALALLAGQALRAVDWTSPLGEPVGVALLQGNVGQE
                     MKFRPEQYARTLETYARLAEETSRRLVVLPETALPQFLDRIDPAYLARLEAAARRNNG
                     DLLVGVPYRTVGGNYYNSVISLGTSPRQIYHKTHLVPFGEFVPPLFGWVMNWLKIYLG
                     DFARGAPDQPPLAAAGQQVAVNICYEDAFGDDIGPRAARATLLANLSNVAWFGDSLAP
                     SQHLQIARLRAIETGRVHLAATNTGITAAIDRDGTVLARLPQFAEGRLEIEVQGYTGV
                     TPYMRFSDWPIVALCLGLLSAATLVAWRRQRR"
     gene            2205..3080
                     /gene="glyQ"
                     /locus_tag="VGO02_12230"
     CDS             2205..3080
                     /gene="glyQ"
                     /locus_tag="VGO02_12230"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEV7478491.1"
                     /translation="MLTFQQLILQLNAFWDRQGCVLLQPYDMEVGAGTFHTATFLRAI
                     GPEPWKAAYVQPSRRPKDGRYGENPNRLQHYYQYQVVLKPSPADIQDLYLESLKNLGV
                     NPQEHDIRFVEDDWESPTLGAWGLGWEVWLDGMEVTQFTYFQQVGGLDCKPVLGEITY
                     GLERLAMYLQDKENVYDLVWTPGVKYGDVYHQNEVEQSRYNFELSNTEMLFRHFGEFE
                     GEAKRLIEAQCVLPAYEMVLKSSHTFNLLDARGAISVTERAAYIGRVRNLAKAVAQAY
                     YDSRDALGFPLLPKT"
     gene            3081..>3784
                     /locus_tag="VGO02_12235"
     CDS             3081..>3784
                     /locus_tag="VGO02_12235"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEV7478492.1"
                     /translation="MAATLLIELLTEELPPKALRELGLNFASSIFEDLAKQGLVAATV
                     TPNINIATPRRLAVIVPAVAEQAAVSEREVQGPSSGAPQAAIAGFAKKHGVAVEALQR
                     QQTPKGEVFVARAKTGGAALKSTLAEIVEKAVKKLPIPRLMRWGSGTEQFVRPVHGLV
                     MLHGTSVVPGKVLGLESGRRTRGHRFMGAGEIELRAADEYESKLSGDGMVIADFGKRK
                     AEIDRQLQAEAKRHSG"
BASE COUNT          603 a         1309 c         1263 g          609 t
ORIGIN      
        1 gctggcgcag ctgctgcgct cggcctactc gcgcaacctg ctcgacgccg acgcgatgtc
       61 gatcatcgag ggcgcgctca ccgtctccga gatgcaggtg cgcgacatca tgatcccgcg
      121 cgcccaggtg gacttcatcg acatcaacga gccggtcgag agcttcatcc cgcaggtcat
      181 ggcgaccgcg cactctcgct tcccggtaat cgaccagaac aaagacgacg tcatcggcgt
      241 gctgttggcg aaggacctgc tgcggcacta cgccggcgag gaggagttca acgtgcgcga
      301 gatgctgcgc ccggcggtgt tcgtgccgga ggcgaagcgc ctgaacgtcc tgctgcgcga
      361 gttccgcgcc agccgcaacc acatggcgat cgtcatcgac gagtacggcg gcgtcgccgg
      421 gctggtgacg atcgaggacg tgctcgagca gatcgtcggc gacatcgagg acgaatacga
      481 cttcgacgag gccgccgaca acatcctgcc cgaggccggc ggccgctggc gcgtcaaggc
      541 gctcacccag atagccgact tcaacgccca cttcggcacg cacttcccgg acgaggcgag
      601 cgacacggtg ggcggcctgg tgatcaagca cctcggccgc ctgccgcggc gcggcgaggt
      661 gcacatcatc gagggcctgc gcttccaggt gctgcgcgcc gacagccggc gcgtgtacac
      721 gctgctcgtc gacaagaaga cgtgacggcg ttcctcgccg gcgcggcggc ggtgccggct
      781 ttcgcgccga tcggcttctt cccgatcgcg ctgcttgcct tcgcctggct cgcccatctc
      841 tgggcgaacg ccagcccgcg gcgcgccttc tggaccgggt ttgccttcgg catgggcctg
      901 ttcggcgccg gcgtgtcctg ggtctacgtc agcctgtcgc agttcggcgg catgccgccg
      961 ccgctcgccg cgctcgccac cttcctcttc tgcgcgctgc tcgcggcatt cacgggcggc
     1021 gccggctggc tgcaggcgcg cgtgcccgcc agcgagccgg tgcgtgcggc gctgctcatc
     1081 ccggcgtcgt ggacgctctt cgaatggctg cgcagctggg tgttcaccgg cttcccgtgg
     1141 ctcgccgccg gctacgccac gaccggctgg ccgctgcagg gcttcgcgcc gctcctcggc
     1201 gtgttcggcg cttcgttcct ggtggtgtcg ctggccggaa tgctctgctg gttcgcccgc
     1261 cggcgcaggg cgctcgccct cgcgccgatc gcgctcgcgc tgctcgccgg gcaagcgctg
     1321 cgcgccgtcg actggaccag cccgctcggc gagccggtgg gcgtcgcgct gctgcagggc
     1381 aacgtcggcc aggagatgaa attccgtccc gagcagtacg cgcgcaccct cgaaacctac
     1441 gcgcgcctcg ccgaggagac gagcaggcgg ctggtggtgc tgccggagac cgcgctgccg
     1501 cagttcctcg acaggatcga tccggcctac ctcgcgcgcc tcgaggcggc ggcgcgccgc
     1561 aacaacggcg acctgctggt cggcgtgccg tatcgcacgg tgggcggcaa ctactacaac
     1621 agcgtaatca gcctggggac gtcgccgcgc cagatctatc acaagacgca cctggtgccg
     1681 ttcggcgaat tcgtgccgcc gcttttcggc tgggtcatga actggctgaa gatctacctc
     1741 ggcgacttcg cgcgcggcgc gcccgaccag ccgccgcttg cggccgccgg ccagcaggtg
     1801 gcggtcaaca tctgctatga ggacgctttc ggcgacgaca tcggcccgcg cgcggcgcgc
     1861 gccaccctgc tcgccaacct gtccaacgtc gcctggttcg gcgattcgct cgcgccttcg
     1921 cagcacctgc agatcgcacg gttgcgcgcc atcgagaccg ggcgcgtgca cctcgccgcg
     1981 acgaataccg ggatcaccgc cgccatcgac cgcgacggca cggtgctcgc gcgcctgccg
     2041 cagttcgccg agggacgcct cgagatcgag gtgcagggct acaccggcgt cacgccgtac
     2101 atgcgcttca gcgactggcc gatcgtcgcg ctctgtctcg gcctgctttc ggctgcgacg
     2161 ctcgtcgcgt ggcgcaggca gcgccggtag aattccagcc tcgcatgctg accttccagc
     2221 agctcatcct gcagctcaac gcattctggg accgccaggg ctgcgtattg ctgcagccct
     2281 acgacatgga agtcggcgcc ggcaccttcc ataccgccac gttcctgcgc gccatcggcc
     2341 ccgagccgtg gaaagcggcc tacgtgcagc cttcgcgtcg tcccaaggac ggccgttacg
     2401 gcgaaaaccc gaaccgcctg cagcactatt accagtatca agtggtgctg aagccctccc
     2461 cggcggacat tcaggatctt tatctcgaat ccctgaagaa cctcggcgtc aatccgcagg
     2521 agcacgacat ccgcttcgtc gaggacgact gggaatcgcc gactctcggc gcctggggcc
     2581 tgggctggga ggtctggctc gacggcatgg aggtcacgca gttcacctac ttccagcagg
     2641 tcgggggcct ggactgcaag ccggtgctcg gcgagatcac ctacggcctc gagcgcctcg
     2701 cgatgtacct gcaggacaag gagaacgtct acgacctggt gtggacaccc ggagtgaagt
     2761 atggcgacgt ctatcaccag aacgaggtcg agcaatcgcg atacaacttc gaattgtcga
     2821 acacggaaat gctgtttcgc cacttcggtg aattcgaagg cgaagccaag cgtctcatcg
     2881 aggcgcagtg cgtgctgccg gcctatgaaa tggtcctcaa gtcctcacac acgttcaatc
     2941 tgctcgacgc gcgcggcgcc atctcggtga ccgagcgcgc cgcctacatc ggccgcgtgc
     3001 gcaacctcgc gaaggctgtg gcccaggcct attacgactc gcgcgacgcc ctcggcttcc
     3061 cgctgttgcc gaagacatag atggctgcca cgctgctcat cgagctgctc accgaggagt
     3121 tgccgccgaa ggcgctcagg gagctcggcc tcaacttcgc ttcttccatt tttgaagacc
     3181 ttgcgaagca aggccttgtc gccgccacgg tcacaccaaa catcaatatc gccacgccac
     3241 ggcggctggc ggtgatcgtg cccgcggtcg cggagcaggc ggcggtctcg gagcgcgagg
     3301 tgcagggacc gtcgagcggc gcgccgcagg cggcgatcgc cggcttcgcg aagaagcacg
     3361 gcgtcgccgt ggaagcgctc cagcgccagc agacgccgaa aggcgaagta ttcgtggcgc
     3421 gcgcaaagac cgggggcgcg gcgctgaaat ccacgctcgc ggagatcgtg gaaaaggcgg
     3481 tgaagaagct gcccataccc aggctcatgc gctggggcag cgggaccgag caattcgtgc
     3541 gtccagtcca cggcctggtg atgctgcacg ggacgagcgt cgttcccggc aaggtgctcg
     3601 ggctcgaatc gggcaggcga acgcgcgggc accgcttcat gggcgcgggc gagatcgagc
     3661 tgcgcgccgc cgacgagtac gagtcgaagc tgagcgggga cggcatggtg atcgccgact
     3721 tcggcaagcg caaagccgag atcgatcgcc agctgcaggc ggaggcgaag cgccacagcg
     3781 gcag
//
LOCUS       DASYJA010000560         3013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1445964, whole genome shotgun sequence.
ACCESSION   DASYJA010000560 DASYJA010000000
VERSION     DASYJA010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445964"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /gene="mrdA"
                     /locus_tag="VGO02_12240"
     CDS             <1..1324
                     /gene="mrdA"
                     /locus_tag="VGO02_12240"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEV7478493.1"
                     /translation="YRGTDFTGRTGVEGSYESELHGTTGFEQVEIDAAGRGIRTLSRS
                     APQSGNNVALTLDIRLQQVAENAFGERRGALVAIEPGTGGVLAFVSKPGFDPNLFVDG
                     IDPQSWAELNNNPDLPLNNRAIAGVYPPGSTFKPFMAMAALELGKRTPRSTINDPGYF
                     FFGDRRFRDSKPGGHGVVDLYKSIVVSSDTFYYQLANDMGIDAIAGFMKNFGLGARTG
                     IDVQGEALGILPSPEWKQARFKNPAQQKWFPGETISIGIGQGYNAYTPIQLAQAMATL
                     AAGGAMYKPHLVAHVTNPRSGEQRYSEPELVRQASFKKDHVEFIKRAMAGVNKEGTGA
                     RSFAGAQYTSGGKTGTAQVIAIKQNEKYDEAKVAERHRDHSLFIAFAPVESPKIALAI
                     IVENGGFGARAAAPIARTVLDYFLLGKAPKGVLLPTPEEEVEEERESD"
     gene            1324..2439
                     /gene="rodA"
                     /locus_tag="VGO02_12245"
     CDS             1324..2439
                     /gene="rodA"
                     /locus_tag="VGO02_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEV7478494.1"
                     /translation="MALESSFTLRRAGVRLAEGIDQPLLLLLVTLSMLGLAALYSAAY
                     ENPARILNQLVSLGVALTAMWIAAQIPPQTLMRLAVPAYVVGLAFLVAVALFGDVVNG
                     ARRWLHVGVTRFQPSEMMKLALPLMLAWYFHKHESALRLRDFALAALLVAVPVALIAR
                     QPDLGTAALVAAAGFYVIFFAGISWRILIGVAVLFAVSLYPLWTHMRDYQKKRILTLL
                     DPTTDPLGAGYHIIQSTIAVGSGGLAGKGWLNGTQSHLEFIPERHTDFIFAVYSEEFG
                     LIGNAVLLILYGLLIGRGLVIASGAATVFARLMAGAIALMFFTYAFVNMGMVSGILPV
                     VGVPLPFLSYGGTALLTLFIGAGILMSVQRHRKLVQT"
     gene            2608..>3013
                     /locus_tag="VGO02_12250"
     CDS             2608..>3013
                     /locus_tag="VGO02_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234096.1"
                     /note="catalyzes the reversible formation of glyoxylate
                     and succinate from isocitrate; glyoxylate bypass pathway;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEV7478495.1"
                     /translation="MTQREKDITAWQKDWKENPRWKGVKRGYDAVDVLALRGSLKIEH
                     TLAKVGAEKLWKKCNEMPFVNSLGALTGNQAMQQVKAGVPAIYLSGWQVAADANDSLS
                     MYPDQSLYAASSVPTVVKRINNALMRADQIQTM"
BASE COUNT          501 a         1043 c          970 g          499 t
ORIGIN      
        1 ctaccgcggc accgacttca ccggccgcac cggcgtggag ggaagctacg agagcgagct
       61 gcacggcacc accggcttcg agcaggtaga gatcgacgcc gccggacgcg gcatccgcac
      121 gctgtcgcgc agcgccccgc aatcgggcaa caacgtcgcg ctgaccctcg acatccggct
      181 gcagcaggtg gccgagaacg ccttcggcga gcggcgcggc gcgctggtgg cgatcgagcc
      241 gggcaccggc ggcgtgctcg ccttcgtctc caagccgggc ttcgatccca acctgttcgt
      301 cgacggcatc gacccgcaga gctgggccga gctcaacaac aatcccgacc tgccgctcaa
      361 caaccgcgcc atcgccggcg tctacccgcc gggctcgacg ttcaagccgt tcatggcgat
      421 ggcggcgctc gagctgggca agcgcacccc gcgcagcacc atcaacgacc ctggctactt
      481 cttttttggc gaccggcgct tccgcgactc caagcccggc ggccacggcg tggtcgatct
      541 gtacaagtcg atcgtggtgt cgtcggacac cttctactac cagctcgcca acgacatggg
      601 gatcgacgcc atcgccggct tcatgaagaa cttcggcctc ggcgcgcgca ccggcatcga
      661 cgtgcagggc gaggcgctcg gcatcctgcc ttcgccggag tggaagcagg cgcgcttcaa
      721 gaacccggcg cagcagaaat ggtttccggg cgagacgatc tcgatcggca tcggccaggg
      781 ctacaacgcg tacacgccga tccagctggc gcaggcgatg gccaccctcg ccgccggcgg
      841 cgccatgtac aagccgcact tggtggcgca tgtcaccaac ccgcgcagcg gcgagcagcg
      901 ctacagcgag cccgagctgg tgcgccaggc gtcgttcaag aaggaccacg tcgaattcat
      961 caagcgcgcc atggccggcg tcaacaagga gggcaccggg gcgcgctcgt tcgccggcgc
     1021 ccagtacacg agcggcggca agaccggcac ggcgcaggtg atcgccatca agcagaacga
     1081 gaagtacgac gaggcgaagg tcgccgagcg ccatcgcgac cattctctgt tcatcgcctt
     1141 cgcgccggtg gaaagcccga agatcgcgct ggccatcatc gtcgagaacg gcggcttcgg
     1201 cgcgcgcgcc gcggcgccga tcgcgcgcac cgtgctcgac tatttcctgc tcggcaaggc
     1261 gcccaagggc gtgctgctgc cgaccccgga ggaagaggtc gaggaagagc gggagagcga
     1321 ctgatggcgt tggaatccag cttcacgctg cgccgcgccg gcgtgcggct cgccgagggc
     1381 atcgaccagc cgctgctgct gctcctggtg acgctctcga tgctggggct cgcggcgctc
     1441 tactccgccg cctacgagaa cccggcgcgc atcctcaacc agctggtgag cctcggcgtc
     1501 gcgctcaccg cgatgtggat cgcggcgcag atcccgccgc agacgctgat gcgcctcgcc
     1561 gtgccggcct acgtcgtcgg cctcgcgttc ctcgtcgcgg tggccctgtt cggcgacgtg
     1621 gtgaacggcg cgcgccgctg gctgcacgtc ggcgtgacgc gcttccagcc ctccgagatg
     1681 atgaagctcg cgctgccgct gatgctcgcc tggtatttcc acaagcacga atcggcgctg
     1741 cgcctgcgcg acttcgccct ggccgcgctg ctggtcgccg tgccggtggc gctgatcgcg
     1801 cgccagcccg acctcggcac cgcggcgctg gtggccgccg ccgggttcta tgtcatcttc
     1861 ttcgccggca tcagctggcg catcctgatc ggggtggcgg tgctcttcgc ggtgagcctg
     1921 tacccgctgt ggacccacat gcgcgactac cagaagaagc gcatcctcac cctgctcgac
     1981 ccgaccaccg atccgctggg cgccggctac cacatcatcc agtcgaccat cgcggtcggc
     2041 tccggcggcc tggcgggcaa gggctggctg aacggcaccc agtcgcacct cgagttcatc
     2101 ccggagcggc ataccgactt catcttcgcc gtgtactccg aggaattcgg cctcatcggc
     2161 aacgccgtgc tgctgatcct ctacgggctt ctcatcggcc gcgggctggt gatcgcctcc
     2221 ggcgccgcca ccgtcttcgc gcgcctcatg gccggcgcga tcgcgctcat gttcttcacc
     2281 tacgccttcg tcaacatggg catggtgtcg ggcatcctgc cggtcgtcgg cgtgccgctg
     2341 cccttccttt cctacggcgg caccgcgctg ctcacgctgt tcatcggcgc cggcatcctg
     2401 atgagcgtcc agaggcaccg caagctggtc cagacgtagt tggtgcgaag cagcattttt
     2461 tgaaagccgc tttcgtatcg tgagatggcc tgccgttaag cctatgtaaa ggcttgtttt
     2521 cttaggtctc aaataggcta catggcgtgt tgctctgcgc cattcccgtc cgcacacttc
     2581 gctgatcctg cataaaccgg aggcagcgtg acgcaacgag aaaaagacat caccgcatgg
     2641 caaaaggact ggaaggagaa cccgcgctgg aagggagtga agcgcggcta cgacgccgtc
     2701 gatgtcctgg cgctgcgcgg cagcctgaag atcgagcaca cgctggcgaa agtcggcgca
     2761 gagaagctgt ggaagaagtg caacgagatg ccgttcgtga actcgcttgg cgcgctcacc
     2821 ggcaaccagg cgatgcagca ggtgaaggcc ggcgtgccgg cgatctatct ctccggctgg
     2881 caagtcgccg ccgacgcgaa cgattcgctg tcgatgtatc ccgaccagtc gctgtacgcg
     2941 gcgtcctcgg tgccgaccgt cgtgaagcgc atcaacaatg cccttatgcg tgcggatcag
     3001 attcagacca tgg
//
LOCUS       DASYJA010000561         5991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2990636, whole genome shotgun sequence.
ACCESSION   DASYJA010000561 DASYJA010000000
VERSION     DASYJA010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5991
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2990636"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..606
                     /gene="gspG"
                     /locus_tag="VGO02_12255"
     CDS             181..606
                     /gene="gspG"
                     /locus_tag="VGO02_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEV7478496.1"
                     /translation="MNKAAKTARNSGFTLLELLVVMVIIGLLAGFVAPRYFGQVGKSE
                     IATARAQIDALEKALDQYRLDVGRYPSTELGLKALLEKPAAEPKWNGPYLRKEVPLDP
                     WGKAYVYKIPGEKHEFDIVSYGKDGQPGGTGENADLTNH"
     gene            624..1808
                     /locus_tag="VGO02_12260"
     CDS             624..1808
                     /locus_tag="VGO02_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV7478497.1"
                     /translation="MRFELKAIAPAGNVEAVDFQAPDETTARRSLEGRGYTVLSVRVK
                     HSLGLPWRSAAERFPVPLFSQELRVLINAGLPLVEAVETLAEKEKRDDFRAVLERLAT
                     VMREGQPLSAALQEFPQAFSPLYVATIRASEKTSDLSDALARYVAYAGQLEAIRKRVV
                     NASIYPALLLAVGGLVSLFLLLYVVPRFGNIYAERGGNLPFFSRVLLTWGQAVDKHGV
                     LVLAFIAGMIGFAIYLFKVFKTQVGNALWRLPAVGEHLKVYQLARFYRTIGMLLRGGM
                     PLVAALDMGAELLHPLLRERLVQAARAISEGRNVSQSMDAAGLTTPVALRMLAVGEKG
                     GNMGEMLEQIAAFHDEELSRWVDWFTRMFEPILMALIGLIIGVIVILMYMPIFELAGG
                     FQ"
     gene            1805..3484
                     /locus_tag="VGO02_12265"
     CDS             1805..3484
                     /locus_tag="VGO02_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEV7478498.1"
                     /translation="MSADASIVAAQIAQARQAAAATRRRVVDVLEEAAGGDPDGFTAR
                     LAASLQLERLAMADLRAAAPAFDLLPFNECSQHGCALLRAADGRLILAFDDPFSGELH
                     AWAEERIAQPFSWGLAHRGDLAAFLGAHEASLRALDEVHGASVAGPADSGRVEDLSLK
                     AIGDESSEVVRLVRSTLRDALMIGASDIHMETLPTGLAIKFRIDGILSQMKQIQDTAQ
                     AEQAIARVKVLAELDITERRVPQDGRFKALERGRAVDFRVSIMPSIYGEDAVLRVLDK
                     QSLYEQTQQQLSLDSLGFEGDEVKRLRRLSREPYGMLLVTGPTGSGKTTTLYAAISEI
                     NTGQDKIVTIEDPVEYQLAGVLQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDPE
                     TANIAVQSALTGHLVFTSVHANNVFDVIGRFMHMNVDLYGFVSALNAILAQRLVRVVC
                     PHCAVPFSPDKELLVESNLDEKQVKGWKFMHGKGCRECRGSGYKGRNAIAELMILNDE
                     LRELITGRAPVRQLKEAAQRSGTRFLRDAAFDAVRQGKTTLQEINRVTFVS"
     gene            3570..4220
                     /locus_tag="VGO02_12270"
     CDS             3570..4220
                     /locus_tag="VGO02_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478499.1"
                     /translation="MVEEKRIACDPVFGAEPWQGAVAGLKSINIDFPAKVTVVLANRF
                     VRYAVVPWSGALGGAAEEEAYLRHHFAKIHGERAKSWALRSSEAAAGAPRLASAIDSA
                     LMQAVRECFPKKGKARLVSVQPQLMTRFNEWRGAVPAAGAWLVLAEADRACIALHSAG
                     RWRSVLSGSGAWLALLERERYRLQGGDAGALPSLVLLAGARTPADKGAWQFHELGA"
     gene            4204..4605
                     /locus_tag="VGO02_12275"
     CDS             4204..4605
                     /locus_tag="VGO02_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478500.1"
                     /translation="MSWARKAALAACLAGLAAAASGGHDNVSVDRALKRYAGKVTAVG
                     TPDGEGRIYSFKARRLQSESAYAPDELRGWRLTMLAGKRFAAAFEVRGNTESEIAVTP
                     LDGPLNGVAAGDVFVVELIAIKRPPRPESQD"
     gene            complement(4602..>5991)
                     /locus_tag="VGO02_12280"
     CDS             complement(4602..>5991)
                     /locus_tag="VGO02_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478501.1"
                     /translation="RTIAFVGTHGGSRPNPLKGGAVEQNGTMIIDVTDPERPVEKFHI
                     PVPVAGGQAQMARMCLGSELPGGLPGRVYLQRNIQGSSAAGYEVWDVTDVTNPQLKGA
                     LRNLRNTHKQWWECNTGIAYMPGSRAVDNWRQGQSMLIVDWKDPANPIYLRTHGLPGG
                     QPSGTGPVPTSLHGPISTHEHPNAAGPLARAATAEDVIGNRVYLAWGVGDDGVLQIVD
                     RRKLLPPPYGTFSGDPNNPTDEQLEAPQTSILYMSPDQGGHTSMPVFGLMPRSYGDFT
                     EFKTRDIVLLASEATSDQCQEAPHWSFIVDVTVENSKTAPPGTRVEPNPWQGPMVLST
                     MSVDPRAGEKYKRGNYCKRGARFGVHSSEENFRNPFYGKLTMLAYFTGGVRVWDIREP
                     QEPVEVGFYVPVSNANTLPAGYMTNNVEVDNRGFIFVVDRNGAGMDILELTGKARQIV
                     FPRGHQNWRDDD"
BASE COUNT          923 a         2019 c         2084 g          965 t
ORIGIN      
        1 tctagaccgc cccgtcgacg cctatgggta gttcccctac cagggggcta ccactctcta
       61 ctgagttgag gctactccac ggtagcaacc gtcccactgg cagtgggtgc cacctcgacc
      121 ggaacatggg gtgaagtgca cattgagcca gaagcgggtg gggtcgacat gcgcgcgtgc
      181 atgaacaagg cggcaaagac cgccaggaat agcggcttta cgttgctcga gctgctcgtc
      241 gtcatggtca tcatcgggct gctggcgggc ttcgtcgcgc cgcgctattt cggacaagta
      301 ggcaagtcgg agatcgccac cgcgcgcgcg cagatcgacg cgctggagaa ggcgctcgac
      361 cagtatcgcc tggacgtcgg ccgctacccg agcaccgagc tcgggctgaa ggcgctgctc
      421 gagaagccgg cggccgagcc gaagtggaac ggcccctacc tgcgcaagga agtgccgctc
      481 gatccctggg gcaaggccta cgtgtacaag atcccgggcg agaagcacga gttcgacatc
      541 gtctcgtacg gcaaggacgg ccagcccggc ggcacgggcg agaacgccga cctcaccaac
      601 cactagcagg gattcgagaa gccatgcgct tcgagctgaa ggccatcgcg ccggcgggca
      661 acgtcgaggc ggtcgatttc caggcgcccg acgagacgac ggcgcggcgc agcctcgagg
      721 ggcgcggcta cacggtgctc agcgtgcgcg tgaagcactc gctcggcctg ccctggcgca
      781 gcgccgcgga gcgcttcccg gtgcccctct tcagccagga gctgcgcgtg ctgatcaacg
      841 ccggcctgcc gctggtggag gcggtcgaga cgctggcgga gaaggagaag cgcgacgact
      901 ttcgcgccgt gctcgagcgc ctcgccaccg tgatgcgcga gggccagccg ctgtcggcgg
      961 cgctgcagga gttcccgcag gccttctcgc ctctctatgt ggccacgatc cgggcgagcg
     1021 agaagaccag cgatctgtcg gacgcgctgg cgcgctacgt cgcctacgcc ggccagctcg
     1081 aggcgatcag gaagcgcgtc gtcaacgcct cgatctaccc cgcgctgctg ctcgccgtcg
     1141 gcggcctggt cagcctgttc ctgctgctgt acgtggtgcc tcgcttcggc aacatctacg
     1201 ccgagcgcgg cggcaacctg ccgttcttct cgcgcgtgct gctcacctgg ggccaggcgg
     1261 tcgacaagca cggggtactg gtgctggcct ttatcgcggg aatgattggt tttgcaatct
     1321 acttgttcaa agtattcaag acccaggtgg gaaatgccct atggcgcctg cccgcggtcg
     1381 gcgagcatct gaaggtgtac cagctcgcgc gcttctaccg caccatcggc atgctgctgc
     1441 gcggcggcat gccgctggtc gccgcgctcg acatgggcgc ggagctgctg catccgctgc
     1501 tgcgcgagcg gctggtgcag gccgcgaggg cgatcagcga agggcgcaac gtgtcgcagt
     1561 cgatggacgc cgccggactt accacgccgg tggccctgcg catgctcgcg gtgggcgaga
     1621 agggcggcaa catgggcgag atgctcgagc agatcgcggc gttccacgac gaggagctgt
     1681 cgcgctgggt cgactggttc acgcgcatgt tcgagccgat cctgatggcg ctgatcggcc
     1741 tgatcatcgg cgtcattgtg atcctcatgt acatgccgat cttcgagctg gcgggaggct
     1801 ttcagtgagc gccgacgcga gcatcgtggc ggcgcagatc gcgcaggcgc ggcaggcggc
     1861 ggcggcgacg cgccggcgcg tcgtcgacgt gctggaggag gcggcgggcg gcgaccccga
     1921 cggcttcacc gcgcgcctcg cggcgagcct gcagctcgag cggctcgcca tggccgacct
     1981 gcgcgccgcg gcgccggcgt tcgacctgct gcccttcaac gagtgctcgc agcacggctg
     2041 cgcgctgctg cgcgccgccg acgggcgtct gatcctcgct ttcgacgacc ccttctccgg
     2101 cgagctgcac gcctgggccg aggagcgcat cgcgcagccc ttcagctggg gcctcgcgca
     2161 ccgcggcgac ctggcggcat tcctcggcgc gcacgaggcg tcgctgcgcg ccctcgacga
     2221 ggtgcacgga gcgagcgtcg ccggcccggc cgacagcggc cgcgtcgagg atctgtcgct
     2281 gaaggcgatc ggcgacgagt cgagcgaggt ggtgcgcctg gtgcgctcga cgctgcgcga
     2341 cgcgctgatg atcggcgcga gcgacatcca catggagacg ctgcccacgg gcctggcgat
     2401 caagttccgc atcgacggga tactctcgca gatgaagcag atccaggaca ccgcgcaggc
     2461 cgagcaggcg atcgcgcgcg tcaaggtgct cgccgagctc gacatcaccg agcggcgcgt
     2521 gccgcaggac ggccgcttca aggcgctcga gcgcggccgc gcggtcgact ttcgcgtctc
     2581 catcatgccg agcatctacg gcgaggacgc ggtgctgcgc gtgctcgaca agcagtcgct
     2641 ctacgagcag acgcagcagc agctctcgct cgactcgctc ggcttcgagg gcgacgaggt
     2701 gaagcggttg cggcgcctgt cgcgcgagcc ctacggcatg ctgctggtga ccggcccgac
     2761 cggcagcggc aagaccacca ccctgtacgc ggcgatcagc gagatcaaca ccggccagga
     2821 caagatcgtc accatcgagg acccggtcga gtaccagctc gccggcgtgc tgcagatccc
     2881 ggtgaacgag aagaagggcc tcaccttcgc gcgcggcctg cgctcgatcc tgcgccacga
     2941 ccccgacaag atcatggtgg gcgagatccg cgacccggag accgccaaca tcgcggtgca
     3001 gtcggccctc accggccacc tggtcttcac ctcggtgcac gccaacaacg tgttcgacgt
     3061 catcggccgc ttcatgcaca tgaacgtcga cctgtacggc tttgtttccg cgctgaacgc
     3121 gatcctcgcg cagcgcctgg tacgtgtggt gtgcccgcat tgcgcggtgc ctttttctcc
     3181 ggacaaggag ctgctggtcg agtccaatct ggacgaaaaa caggtcaagg gctggaaatt
     3241 catgcacggc aagggctgcc gcgagtgccg cggctcgggc tacaagggac gcaacgccat
     3301 cgccgagctg atgatcctga acgacgagct gcgcgagctg attaccggcc gcgcgccggt
     3361 acggcagctg aaggaagcgg cgcagcgctc gggaacgcgc ttcctgcgcg acgcggcctt
     3421 cgatgcggtg aggcagggaa agacaacgct ccaggaaatc aaccgtgtta cgtttgtttc
     3481 ttaaaagact gtttcccgac cggctgattc tttcgcttgc tccttctgag atttttcttc
     3541 tcagagaaac ttcctttcca aagcgaagga tggtcgagga gaagcgcatc gcctgcgacc
     3601 cggtgttcgg cgccgagccg tggcagggcg cggtggcggg gctgaaatcg atcaacatcg
     3661 acttccccgc gaaagtgacc gtcgtcctcg ccaaccgctt cgtgcgctac gcggtggtgc
     3721 cgtggagcgg cgcgctgggc ggcgcggcgg aagaggaagc ctacctgcgc caccacttcg
     3781 cgaagatcca cggcgagcgc gccaaaagct gggcgctgcg ctcgagcgaa gccgcggccg
     3841 gcgcgccgcg cctggcgagc gccatcgaca gcgcgctgat gcaagccgtc agggaatgct
     3901 tcccgaagaa aggcaaggcg aggctcgtct ccgtccagcc gcagctcatg acgcgcttca
     3961 atgaatggcg cggcgcggtg cctgccgccg gcgcgtggct ggtcctcgcc gaagccgacc
     4021 gcgcctgcat cgcgctgcat agcgccgggc gctggcgctc ggtgctgagc ggcagcggcg
     4081 cctggctcgc cctgctcgag cgcgagcgct accgcctgca gggcggcgac gccggcgcgc
     4141 tgcccagcct ggtgctcctc gccggcgcgc gcacgccggc ggacaaggga gcctggcagt
     4201 tccatgagct gggcgcgtaa ggccgcgctc gcggcctgcc tcgcgggact ggcggcggcc
     4261 gcgagcggcg gccacgataa cgtcagcgtc gaccgcgccc tcaagcgcta cgccggcaag
     4321 gtgaccgcag tgggcacgcc cgacggcgaa ggccgcatct actccttcaa ggcgaggcgc
     4381 ctgcagtccg agtcggccta tgcgccggac gagctgcgcg gctggcgcct caccatgctc
     4441 gccggcaagc gcttcgccgc ggcgttcgag gtgcgcggca ataccgagtc cgagatcgcg
     4501 gtcacgcctc tggatggacc gctcaatggc gtggcggcgg gcgacgtgtt cgtcgtcgag
     4561 ctgatcgcca tcaagcggcc tccccggccg gaaagccagg actagtcgtc gtcgcgccag
     4621 ttctggtggc cgcgcgggaa cacgatctgc ctcgccttgc cggtcagctc gaggatgtcc
     4681 atccccgcgc cgttgcggtc gaccacgaag atgaagccgc ggttgtcgac ctcgacgttg
     4741 ttggtcatgt agccggcggg cagcgtgttg gcgttgctca ccgggacgta gaagcccacc
     4801 tcgaccggct cctgcggctc gcggatgtcc cacacccgaa ccccgccggt gaaataggcg
     4861 agcatcgtca gcttgccgta gaacgggttg cggaagttct cctcgctcga atgcaccccg
     4921 aaacgcgcgc cgcgcttgca gtagttgccg cgcttgtact tctcgcccgc acgcgggtcc
     4981 accgacatcg tcgagagcac catcggtccc tgccacggat tcggctccac gcgggtgccg
     5041 ggtggcgcgg tcttcgagtt ctcgaccgtg acgtcgacga tgaagctcca gtgcggcgct
     5101 tcctggcact ggtcggaggt cgcctccgag gcgagcagca cgatgtcgcg cgtcttgaac
     5161 tcggtgaagt cgccgtagct gcgcggcatc aggccgaaca ccggcatgct ggtatggcct
     5221 ccctggtcgg gcgacatgta caggatcgag gtctgcggcg cctcgagctg ctcgtcggtc
     5281 gggttgttcg ggtcgccgct gaacgtgccg tagggcggcg gcagcagctt cctgcggtcg
     5341 acgatctgca gcacgccgtc gtcgcccacg ccccatgcca ggtagacgcg attgccgatg
     5401 acgtcctcgg ccgtcgcggc gcgcgccagc gggcccgccg cattgggatg ctcgtgcgtc
     5461 gagatcgggc cgtgcagcga ggtcggcacc gggccggtgc cgctcggctg cccgccgggc
     5521 aggccgtgcg tgcgcaggta gatcgggttg gccgggtcct tccagtcgac gatcagcatc
     5581 gattggccct gccgccagtt gtcgaccgcc ctgctccccg gcatgtatgc gatgccggtg
     5641 ttgcactccc accactgctt gtgcgtgttg cgcaggttgc gcagcgctcc cttgagctgc
     5701 gggttggtga cgtcggtcac gtcccagacc tcgtagcccg cggcgctgct gccctggatg
     5761 ttgcgctgga gatagacgcg gccgggcagg ccgccgggca gttccgagcc gaggcacatg
     5821 cgcgccatct gcgcctgccc gccggccacc ggcaccggga tatggaactt ctccaccggc
     5881 cgctccggat cggtgacgtc gatgatcatg gtgccgttct gctcgacggc gccgcctttc
     5941 agggggttgg gccggctgcc gccgtgcgtg ccgacgaacg cgatcgtcct g
//
LOCUS       DASYJA010000562         3125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1627824, whole genome shotgun sequence.
ACCESSION   DASYJA010000562 DASYJA010000000
VERSION     DASYJA010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627824"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VGO02_12285"
     CDS             complement(<1..233)
                     /locus_tag="VGO02_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478502.1"
                     /translation="MSHERDAAFHLGLQLGPLAWSSSIGIAALRVVAWLLPSQYSMTA
                     LILSLAAAVALATALGCVAALARRRPPSERDLVR"
     gene            271..1518
                     /locus_tag="VGO02_12290"
     CDS             271..1518
                     /locus_tag="VGO02_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478503.1"
                     /translation="MSRRVRFALSLLVAVPATAFAFDSVDLIPYTTSGAFPAYPGDPL
                     RPLSGFLEIGVEHDSNPFRLSDSLSPASDNVLRYGGGARYQGRVIGRQSILLEGRGDY
                     YDYQKSDALDHFAYSLLGEWQWELGNQLSGAVGAGRVYRIADPAQIQREIRQDVTIDR
                     AYADGGWLFATNWRLRAGVAADRSTRQRPGAAEVRADSQTARVGLDYVTPLANTFGIE
                     ARASKGDAPVSDTFDPNGEFTANDYREKEISAVVSYGATSQFRVGGRVGHTERTFTEV
                     TGHDFSGTTWRALVEWLPGNKTILGFESYKYPTSIIDVDAAYVVRQGSAFTLSWAPLA
                     KLVFSGRLFEERRQSIGTPEAILLGTPQRDDTAHGARVGVGWEPVRFCEVGFGVEAGK
                     RTSTVVLRDYDYTTVSANLRIRF"
     gene            complement(1543..2964)
                     /locus_tag="VGO02_12295"
     CDS             complement(1543..2964)
                     /locus_tag="VGO02_12295"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEV7478504.1"
                     /translation="MIQPVILCGGSGTRLWPLSREHYPKQLHALNGERTLLQETAARC
                     EGLAAGGDPLIVCNENHRFMVADQLRRAGTRPRAILLEPHGRGTAPALTLAAVHAVTA
                     DDPVLLAMPSDHVLTQHAEFRRLAERAAALARQGYMVTFGVPPTSPETGYGYIRAGRG
                     IDASVSFVEAFVEKPEAAAAAEYLASGRFLWNAGIFAVRASVWIDAIGNWRRDILAAC
                     ERAYRQGSRSGAFWKMDAEAFAACPADSIDYAVMEKIAGGSSGLEAVVMRLDAGWSDV
                     GAWDALWDIGEKDSHGNVVHGDVHAADTKNSLLISQSRLVTCIGLEDVVVVETPDAVM
                     VAHKDKAQAVGAVVSKLKAAGREETRAHRKVHRPWGTYDDLERGERFRVKRIVVEPGA
                     ALSLQMHHHRAEHWVVVRGTAHVVRGEETFLLTENESTYIPPGTRHRLENPGKLPLEI
                     IEVQSGAYLGEDDIVRFEDQYGR"
BASE COUNT          480 a         1103 c         1056 g          486 t
ORIGIN      
        1 cgcaccaggt cgcgctcgct cggcggccgc cggcgcgcga gcgccgcgac acagcccagc
       61 gcggtcgcca gcgcgaccgc ggcggcgagc gagaggatca gcgctgtcat cgaatattga
      121 ctcggcagta gccaggcaac gacacgcagc gcggctatgc cgatggaact actccaggcg
      181 agcggaccaa gctgcaagcc gaggtggaac gccgcgtcgc gctcatgcga cattcgagac
      241 tccttcaacg atacgaaaaa cccgtcttcc atgtcccgac gcgtccgttt tgcgctctcg
      301 ctgctggtcg ccgtccctgc gacggccttc gctttcgatt ccgtcgacct gattccctac
      361 accacgagcg gcgcgttccc ggcctatccc ggcgaccccc tgcggccgct cagcgggttc
      421 ctggaaatcg gcgtcgagca cgacagcaat cccttccggc tgtccgacag cctcagccct
      481 gcctccgaca acgtcctgcg ctatggcggc ggcgcccgct accagggaag agtcataggc
      541 cggcagagca tcctgctgga aggccgcggc gactactacg actaccagaa gtccgacgcg
      601 ctggaccatt tcgcctacag cctgctcggc gaatggcagt gggagctcgg caaccagctc
      661 tccggcgccg tgggcgccgg gcgcgtctac cgcatcgcag accctgcgca gatccagcgc
      721 gagatccggc aggacgtcac catcgaccgc gcctacgcgg acggcggctg gctgttcgcg
      781 acgaactggc gcctgcgggc cggcgtcgca gccgacagga gcacccggca gcgccccggg
      841 gccgccgagg tccgcgcgga ctcccagacg gcgcgcgtcg ggctggacta cgtgacgccg
      901 ctcgccaaca ccttcggcat cgaggcgcgc gccagcaaag gcgacgcgcc ggtgagcgac
      961 acattcgatc cgaacggcga gttcaccgcc aacgactacc gcgagaagga gatctcggcg
     1021 gtggtgtcct acggcgcaac gtcgcagttt cgcgtcggcg gccgcgtcgg ccacacggag
     1081 cgcaccttca ccgaggtgac cggccacgat ttcagcggca cgacctggcg cgcgctcgtc
     1141 gaatggctgc ccggcaacaa gaccatcctc ggcttcgaga gctataaata tccgacatcc
     1201 atcatcgacg tcgacgcggc ctacgtcgtg cggcagggca gcgcgttcac cctcagctgg
     1261 gcgccgctcg ccaagctggt gttctccgga cgcctgttcg aggagcgccg ccagtcgatc
     1321 ggcacgcccg aggcgatcct gctcggtacg ccgcagcgcg acgacacggc ccatggcgcg
     1381 cgcgtgggag tcggctggga gccggtgcgc ttctgcgagg tgggcttcgg cgtcgaagca
     1441 ggcaagcgca cctcgaccgt ggtgctgcgc gactacgact acacgacggt ctcggctaat
     1501 ttgaggatcc gtttctagcg gggcttcttc cgcacctgac ttctaacggc cgtattggtc
     1561 ttcaaaacgg acgatgtcgt cttctcccag ataggcaccc gattgcacct cgatgatctc
     1621 gaggggtagc tttccggggt tctccagccg gtgccgcgtg cccggcggga tgtaggtcga
     1681 ttcattctcc gtgaggagga aagtctcctc gccgcgcacc acgtgcgccg tgccgcgcac
     1741 tactacccag tgctcggcgc ggtggtggtg catctgcagc gagagggccg cgcccggctc
     1801 gaccacgatg cgcttgacgc ggaagcgctc gccgcgctcg aggtcgtcgt aggtgcccca
     1861 ggggcggtgc accttgcggt gcgcgcgcgt ttcctcgcgg ccggcggcct tgagcttcga
     1921 taccaccgcg cccaccgcct gcgccttgtc cttgtgcgcc accatcaccg cgtcgggcgt
     1981 ttcgaccacc accacgtcct ccaggccgat gcaggtgacc aggcggctct gcgagatgag
     2041 cagcgagttc ttcgtgtcgg cggcgtgcac gtcgccgtgc accacgttgc cgtgcgagtc
     2101 cttttctccg atgtcccaca gcgcatccca cgcgccgacg tcggaccatc cggcgtcgag
     2161 gcgcatgacg actgcctcaa gaccggatga tccgccggcg atcttctcca tgaccgcata
     2221 gtcgatcgag tccgccgggc aggcggcgaa ggcctcggca tccatcttcc agaaggcgcc
     2281 gctgcggctg ccctggcggt aggcgcgctc gcaggcggcg aggatgtcgc gccgccagtt
     2341 gccgatggcg tcgatccaca cggaggcgcg cacggcaaag atgccggcgt tccacaggaa
     2401 gcggcccgac gcgaggtact cggcggcggc cgcggcctcg ggcttctcga cgaaggcctc
     2461 gacgaaagaa acgctggcgt cgatccctcg gccggcgcgg atgtagccgt agccggtctc
     2521 gggcgaggta ggcggcacgc cgaaggtcac catgtagccc tgccgcgcga gcgcggcggc
     2581 gcgctcggcg aggcggcgga actccgcgtg ctgcgtcagc acgtggtcgg agggcatcgc
     2641 gagcagcacc gggtcgtcgg cggtcacggc gtgcactgcg gcaagcgtca gcgccggggc
     2701 cgtgccgcgg ccgtgcggct cgagcaggat ggcgcgcggc cgggtgccgg cgcggcgcag
     2761 ctggtcggcg accatgaagc ggtggttctc gttgcagacg atcagcggat cgccgccggc
     2821 ggccagcccc tcgcagcgcg ccgcggtctc ctgcagcagc gtgcgctcgc cgttcagcgc
     2881 gtgcagctgc ttgggatagt gctcgcggga aagcggccaa aggcgcgtgc ccgagccgcc
     2941 gcacaggatc accggctgga tcatcgtggg ggtcacgccc agctcgatca ctgcttctcc
     3001 ttgttctaga agctagaggg ctctcgcgta gcgccgccgc tcgccgaaca gcctctggta
     3061 gctccagtct gcgccgaagc gcgcgtggta gtccgcctgg gcgcactccc ccatctggcg
     3121 catct
//
LOCUS       DASYJA010000563         7802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_84960, whole genome shotgun sequence.
ACCESSION   DASYJA010000563 DASYJA010000000
VERSION     DASYJA010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7802
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84960"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VGO02_12300"
     CDS             <1..292
                     /locus_tag="VGO02_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7478505.1"
                     /translation="TFRAPESRPDHHCYETAGWMDIRDWGDRMASLRIPLWWGPGRHG
                     PGNNLFFMVEDPDGYKVEFSAELELIPKEVPYRTWPHEQRTLNLWGSAWMRS"
     gene            complement(258..1460)
                     /locus_tag="VGO02_12305"
     CDS             complement(258..1460)
                     /locus_tag="VGO02_12305"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HEV7478506.1"
                     /translation="MEFRVRATFPAQASDNKKPKGNVALTRNFDFDSPVDRAGTWSTR
                     WERHAGRDVIPLWVADSDFRAPPAVLEALAERVRHGVLGYTAPPEELRRAIVDRLERL
                     YRWRIDPAAIVFLPGVIAGLHHAARVLVAPGERILVPRPVYHHLKRAPELAPRPYGEA
                     PLVLQAGRWVFDEDVIKKNSHARAFYLCNPQNPGGTIFRRQELERLAELTRDMLIVSD
                     EIHCDLQLDAGKPHIPIASLDPEVSKRTVTLMSPNKAFNFPSAGCAFAVIENPDLRKR
                     FSADVDLHVMPSSSVFGYVAALAAYRDGQPWLDAQIEYLRGNRDLVEKTIGLPVAHVE
                     ATYLAWIDCSSLENALELFLKNGVALSPGSQFGDARFVRLNFGTQRALLKKALERIKN
                     ASRQSPRG"
     gene            complement(1508..3001)
                     /locus_tag="VGO02_12310"
     CDS             complement(1508..3001)
                     /locus_tag="VGO02_12310"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEV7478507.1"
                     /translation="MSAEILIGLVMLVVMLFAIFIGVPISFTLLFLALTFGYAALGKV
                     VFDLAYFQTIGMMKEEVLAAVPLFIFMGFITEQAGLMERLFSALRMMLAPVRGSLYIV
                     VILTSTVFAMATGIVGAAVTVLGIMAAPIMIKSGYDAKLSAGAITAGGTLGILIPPSV
                     MLIVMGPVLGVSVADLYAAAFGPGFLLAGLYLVYLIGRSWINPKLGPPVPMDERVTGI
                     YPIMREVVIGVLPLFLLIGFTLGSILMGLATPTEAAGIGALGALILAVAYRRLNYAGL
                     RRAVISAMTTSSMVLLLAVTSNIFGAVFARMGTANWITNAMVSMPVPPIVMLGFVIIL
                     IFLLGWPFEWPAIILVFLPIFYPVVEALRPALSQQMGIPPDLFMVWFGALVAVTLQTA
                     YLSPPVAMSAYYLKQVVKEWSLLTIYKGMFEFMVLQCIAIVVVMFVPQIATSFPEQLQ
                     HEARQVQTEEVDDSQNRLEEDPLKQGMEQQEEEAGSSLEKDELPKKK"
     gene            complement(2998..3549)
                     /locus_tag="VGO02_12315"
     CDS             complement(2998..3549)
                     /locus_tag="VGO02_12315"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEV7478508.1"
                     /translation="MAEQSPGLIKAVRVIDKFTDTTGVWVAWLNVPLVLAVAYEVIAR
                     YFFHAPTIWSFDVTYMLYGTIFMLGCAFALHKGAHIRTDFLYDRWSVRTRGVIDAISY
                     IVFFFPSFFVFMLVGWEEGFYSFNISETSEQTPWRPILWPFKMVVPLACLLLLVQGIS
                     ELIKSFYAAQTGVMLEHKEKIEI"
     gene            complement(3613..4731)
                     /locus_tag="VGO02_12320"
     CDS             complement(3613..4731)
                     /locus_tag="VGO02_12320"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEV7478509.1"
                     /translation="MRHIVHSAAALVCAAAFSSAAPAQTVTLKMQATWPASLTLYENF
                     TYFAERVGKISAGTLKIDAMPAGQVVPAFEVLDATHKKVLDGAHAWSGYWVGKNKAAI
                     LCTGGPGGTFGMDMIDAIGWMHHGGGNEFCNEFFQKELKLNVHWMPILPSGPQAFGWF
                     KRPVKNLADFKGMKCRQTGMAAEVWQRMGMTTVNMPGGEIIPAAQRGVIDCAEWVGGV
                     EDLRLGFHNVWKYHYTPGMHENVTIGEVVFNMDVWKGLSVQHQEMIKSAANETFIIWW
                     AKWQKQNAEALVELQTKHGVRVLRTPPDILVEFLKTWDIIAAEEGKKSPFFKKVHDSQ
                     RAYASVVVPAKRFYFPPYSFAANYYWPEGSAKKAPADK"
     gene            complement(4807..5937)
                     /locus_tag="VGO02_12325"
     CDS             complement(4807..5937)
                     /locus_tag="VGO02_12325"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEV7478510.1"
                     /translation="MKRAFSLTALAAAAALAFTANAQQPAAPAVKQLKMQSTWPASLT
                     LQDNFRLFAERFDKLTRGEVKIEAMAAGQIVPAFEILDATHKKVLDGGHGVAYYWVGK
                     NKAATLFSATPAGPFGMDHFDFLGWMQEGGGQALYDEFYRDVLKLNVLGMPIGGASPQ
                     AFGWFKRPIKDLADFKGMKCRQTGIVAEIYQKMGMQTVNMPGGEIVPSAQRGVIDCAE
                     WVGGIEDLRLGLPQVWKYHYTPGMHESNSMLEVIVNGDVWKSFTPHQQEAWRAAQMET
                     FVRWGVRWQKQNADAIEEMRTKHGTQILRTPPAILIEFMKTWDKMAAEESAKDPFFKK
                     TLESQRVYAAKVVPAKRFMYPPYSFAANYYFPEEKKGAAQKK"
     gene            5994..7193
                     /locus_tag="VGO02_12330"
     CDS             5994..7193
                     /locus_tag="VGO02_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEV7478511.1"
                     /translation="MKITKLETLRIAEFPKIVWLRVHTDEGLTGLGETSYAAGTVETY
                     LHEHVAPRALGRDPLAVESLARSLQDYLGYRSSGVETRALSALDLALWDLFGKSCNLP
                     VYQMLGGASRQSVRTYNTCAGYRYARDTRGQVSANWGLDGKSEGPYEDLDAFLHRADE
                     LAHSLLEQGITAMKIWPFDTYAEASDGWHISGSDLQESLEPFRRIRKAVGDKMDIMVE
                     FHSLWRLPAAQRIARALAEFDTYWHEDPIRMDSLDLLKQYAAHSKAPICGSEILAGRW
                     SFKDFLETGVAGIVMFDLGWCGGLTEARRIAALADAWQLPVAPHDCVGPVVFAASLHF
                     SIWAPNALVQESVRAFYTGWYKELVMNVPQPVRGFFEKPSGPGLGCELLPEVWKRKDA
                     VVRVSPA"
     gene            complement(7180..>7802)
                     /locus_tag="VGO02_12335"
     CDS             complement(7180..>7802)
                     /locus_tag="VGO02_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEV7478512.1"
                     /translation="GFSAAMAAKRGRDHLRCPTQGLELLGVDSKKINHVVITHLHYDH
                     VGNFDLFPAATLHLQELEMRYATGRYMCQDCFRGAFEVEDVVGMVREVYAGRVRFHDG
                     DAELSPGVSLHLIGGHTMGLQVVRVATRRGWVVLAADASHFYANMEQVRPFPIVWSVA
                     DMVDGYGKLRSLAESPQHIIPGHDPLVMQRYPAPSKALEGIAVRLD"
BASE COUNT         1401 a         2534 c         2495 g         1372 t
ORIGIN      
        1 gaccttccga gcacccgaat cccgccctga ccatcactgc tacgagacgg ccggctggat
       61 ggacatccgc gactggggcg accgcatggc gagcctgcgc attccgctgt ggtggggacc
      121 cgggcgccat gggccgggca acaacctgtt cttcatggtc gaggatcccg acggctacaa
      181 ggtcgagttc tcggccgagc tcgagctgat cccgaaggaa gttccctatc gcacctggcc
      241 ccacgagcag cgcacgctca acctctgggg ctctgcctgg atgcgttctt gattcgttcg
      301 agagctttct tcagcagagc tctttgagtc ccgaagttca gccgcacgaa gcgcgcgtcg
      361 ccgaactgcg agccgggcga gagcgcgacg ccgttcttga ggaatagctc cagggcattc
      421 tccaggctgg agcagtcgat ccacgcgagg tacgtcgcct cgacgtgcgc caccggtaga
      481 ccaatggttt tctccacaag gtccctgttg cccctgaggt actcgatctg cgcgtcgagc
      541 cacggttgcc catcccggta tgcggcaagc gcagcgacat agccgaatac cgaggacgac
      601 ggcatgacgt gcaggtcgac gtccgcggag aatctcttgc gcagatcggg attctctatc
      661 accgcaaagg cgcaacccgc cgagggaaag ttgaacgcct tgttcggcga catcaaggtg
      721 acggtgcgtt tcgacacctc ggggtcgagg ctggcgatcg ggatgtgcgg cttgcccgcg
      781 tcgagctgca ggtcgcagtg gatctcgtcg gagacgatca gcatgtcgcg cgtcagctcg
      841 gcgagccgct cgagctcctg gcggcggaag atggtcccgc cggggttctg cggattgcac
      901 agatagaagg ctcttgcatg cgagtttttc ttgatgacat cttcgtcaaa cacccagcgg
      961 ccggcctgca gaacgagcgg agcttcaccg tagggccttg gcgcaagctc aggggcgcgc
     1021 ttgaggtggt gatagacggg cctgggaacc aatatccttt ccccgggagc aaccagcacg
     1081 cgcgccgcat ggtgcaggcc ggcgatgacg ccgggcagga acacgatcgc ggccggatcg
     1141 atccgccagc ggtaaagccg ctcgagccga tcgacgatcg cccggcgcag ctcctcgggc
     1201 ggcgcggtat agccgagcac gccgtgccgg acccgttcgg cgagcgcctc cagcaccgcc
     1261 ggcggcgcac ggaaatcgga atcggccacc cacaggggga tcacatcgcg gccggcgtgg
     1321 cgctcccagc gcgtgctcca ggtgccggcg cggtcgacgg gtgagtcgaa gtcgaaattc
     1381 cgggtcagag caacatttcc tttcggcttc ttattatcgg atgcttgtgc gggaaatgtt
     1441 gctctgaccc ggaattccaa aaaaaaacgc cgggcgcgag cccggcgctt cgagtgtacg
     1501 gcgatctcta cttcttcttc ggcagctcgt ccttctcgag gctgctgccc gcctcctcct
     1561 cctgctgttc catgccctgc ttgaggggat cctcctcaag acggttctgg ctgtcgtcga
     1621 cttcctcggt ctgcacctgg cgcgcctcgt gctgcagctg ctccgggaac gaggtggcga
     1681 tctgcggcac gaacatcacc acgacgatcg cgatgcactg caggaccatg aattcgaaca
     1741 tgcccttgta gatggtaagg agcgaccatt ccttcaccac ctgcttgagg taataggccg
     1801 acatcgccac cggcggcgac aggtaggcgg tctgcaacgt gacggcgacc agcgcgccga
     1861 accagaccat gaagagatcc ggcgggatgc ccatctgctg cgacagcgcc gggcgcagcg
     1921 cctcgaccac cggatagaag atcggcagga acaccaggat gatcgccggc cattcgaacg
     1981 gccagccgag caggaagatg aggatgatga cgaagccgag catcacgatc ggcggcaccg
     2041 gcatcgacac catcgcattg gtgatccagt tggccgttcc catgcgggcg aacaccgcgc
     2101 cgaagatgtt ggaagtcacg gcgagcagca gcaccatgct ggaggtagtc atcgcactga
     2161 tcaccgcccg cctcaacccc gcgtagttca gcctgcggta agcgactgcc aggatcagcg
     2221 cgccgagcgc gccgatgccg gccgcttcgg tcggggtcgc cagtcccatc aggatcgagc
     2281 ccagggtaaa gccgatgaga aggaacagcg gcaggacgcc gatcaccacc tctctcatga
     2341 tcgggtagat gccggtgacg cgctcatcca tcggcaccgg cggtcccagc ttcggattga
     2401 tccagctgcg gccgatcagg tagaccagat acaggccggc gagcaggaag ccggggccga
     2461 atgccgccgc gtacaggtcg gcgaccgaca cgccgagcac cgggcccatc acgatcagca
     2521 tcaccgacgg cggaatcagg atgccgagcg tgccgccggc agtgatggcg ccggccgaga
     2581 gcttggcgtc gtagccgctc ttgatcatga tcggcgccgc catgatgccg agaaccgtca
     2641 ccgccgcgcc gacgatgccg gtggccatcg caaacacggt agaggtcagg atcaccacga
     2701 tgtagagcga gccgcgcacc ggcgcgagca tcatgcgcag cgccgagaat aatcgctcca
     2761 tcaggccggc ctgctcggtg atgaacccca tgaagatgaa caggggcacc gcggcgagca
     2821 cctcctcctt catcatgccg atggtctgga agtaggcgag gtcgaacacc accttgccca
     2881 aagccgcgta gccgaaggtg agcgcgagga acagcagcgt gaaggaaatc ggcacgccga
     2941 tgaagatggc gaacagcatc accaccagca tgaccaggcc gatcaggatc tcggcgctca
     3001 tatctcgatc ttttccttgt gctcgagcat cacgccggtc tgcgcggcgt agaagctctt
     3061 gatcagctcg gagatcccct gcacgagcag gagcaggcag gcgagcggca ccaccatctt
     3121 gaacggccac aggatcggcc gccagggtgt ctgctcggag gtctcgctga tgttgaagga
     3181 gtagaagccc tcctcccagc cgaccagcat gaagacgaag aacgagggga agaagaacac
     3241 gatgtaggag atggcgtcga tgacgcccct ggtcctgacc gaccagcggt catagaggaa
     3301 gtcggtgcgg atgtgcgcgc ccttgtgcag cgcgaaggcg cagccgagca tgaagatcgt
     3361 gccgtagagc atgtaggtga cgtcgaacga ccagatggtc ggcgcgtgga agaagtagcg
     3421 cgcgatcacc tcgtaggcca ccgccagcac cagcggcacg ttcagccagg ccacccagac
     3481 gccggtggtg tcggtgaact tgtcgatgac gcggaccgcc ttgatcaggc ccggggactg
     3541 ctcggccatc ttctcccctt ctcctataaa ttgaaacggg ggctctcgcc cccgtctgcg
     3601 gattgtcgaa tcctacttgt cagccggcgc cttctttgca ctgccttcgg gccaatagta
     3661 gttggcggcg aaggagtacg gcgggaagta gaagcgcttc gccggcacga cgaccgaagc
     3721 gtacgcgcgc tgcgagtcgt gcactttctt gaagaacggg ctcttcttgc cttcctcggc
     3781 ggcgatgata tcccaggtct tcaggaactc gaccaggata tcgggcgggg tgcgcagcac
     3841 gcgcacgccg tgcttcgtct gcagctcgac cagcgcctcg gcgttctgct tctgccactt
     3901 ggcccaccag atgatgaagg tctcgttggc ggcgctcttg atcatttcct ggtgctggac
     3961 cgagaggccc ttccacacgt ccatgttgaa cactacttcg ccgatggtga cgttctcgtg
     4021 catgccgggc gtgtagtggt acttccagac gttgtggaaa cccaggcgca ggtcttcgac
     4081 gccgccgacc cactccgcgc agtcgatcac gccacgctgc gccgccggga tgatctcgcc
     4141 gccgggcatg ttgacggtgg tcatgcccat gcgctgccat acttccgccg ccatgccggt
     4201 ctgccggcac ttcatgccct tgaagtcggc caggttcttc accggccgct tgaaccagcc
     4261 gaaggcctgg ggccccgagg gcaggatcgg catccagtgc acgttgagct tgagctcttt
     4321 ctggaagaac tcgttgcaga actcgttgcc gccgccgtgg tgcatccagc cgatggcgtc
     4381 gatcatgtcc atgccgaagg tgccgccggg accgccggtg cacaggatcg cggccttgtt
     4441 cttgccgacc cagtagcccg accaggcgtg cgcgccgtcg agcactttct tgtgggtggc
     4501 gtcgagcacc tcgaaggccg gtaccacctg ccctgcaggc atggcgtcga tcttcagcgt
     4561 gccggcggaa atcttgccga cgcgctcggc gaagtaggtg aagttctcgt agagcgtcag
     4621 gctggcgggc caggtcgcct gcatttttag tgtgacggtt tgcgcgggcg cggcgctgct
     4681 gaacgcggcg gcgcagacga gcgctgcggc cgaatgaacg atgtgcctca tgacccctcc
     4741 tctaaggttt cgctaggcga aagcagccta caacgccccg gggcggcagg tcaacgggaa
     4801 tacaggctat ttcttctgcg ccgcgccttt cttttcctcg gggaagtagt agttcgccgc
     4861 aaaagagtaa ggcgggtaca tgaagcgctt ggccggcacc actttcgccg catagacgcg
     4921 ctgcgactcc agcgtcttct tgaagaacgg gtccttggcc gactcctcgg ccgccatctt
     4981 gtcccaggtc ttcatgaact cgatcaggat cgccggcggc gtgcgcagga tctgcgtgcc
     5041 gtgcttggtg cgcatttcct cgatggcgtc cgcgttctgc ttctgccagc gcacgcccca
     5101 gcgcacgaaa gtctccatct gcgccgcgcg ccacgcctcc tgctgatgcg gcgtgaagct
     5161 cttccacacg tcgccgttca cgatcacctc gagcatcgag ttcgactcgt gcatgccggg
     5221 cgtgtaatgg tacttccaca cctgcggcag gccgaggcgc aggtcttcga tcccgcccac
     5281 ccattcggcg caatcgatca cgccgcgctg cgcggaaggc acgatctcgc cgccgggcat
     5341 gttgaccgtc tgcatgccca tcttctggta gatctcggcg acgatgccgg tctggcggca
     5401 cttcatgccc ttgaagtcgg cgaggtcctt gatcggccgc ttgaaccagc cgaaggcctg
     5461 cgggctcgcg ccgccgatcg gcatgccgag cacgttgagc ttgaggacat cgcggtagaa
     5521 ctcgtcgtag agcgcctggc cgccgccttc ctgcatccag ccgagaaagt cgaagtggtc
     5581 catgccgaaa gggccggcgg gcgtggccga gaacagcgtc gccgccttgt tcttgcccac
     5641 ccagtagtag gcgacgccgt ggccgccgtc gagcaccttc ttgtgcgtgg cgtcgaggat
     5701 ctcgaaggcc ggcacgatct ggcccgcggc catcgcctcg atcttcacct cgccgcgcgt
     5761 cagcttgtcg aaccgctcgg cgaacaggcg gaagttgtcc tgcagcgtca ggctcgccgg
     5821 ccaggtcgac tgcatcttca gctgcttgac ggccggcgcc gccggttgct gcgcgttcgc
     5881 agtgaacgcc agcgccgccg ccgcggccaa tgccgtaagc gaaaatgctc gtttcatgag
     5941 ttcctcctcc tcagggggag caagatgtta ggagtgccac gcatggagtg tcaatgaaga
     6001 tcaccaagct cgagacgctg aggatcgcgg agtttccgaa gatcgtctgg ctgcgcgtgc
     6061 atacggacga ggggctcacc ggactgggcg agaccagcta tgccgcgggg actgtcgaga
     6121 cctatctgca cgagcacgtg gcgccgcgcg ccctcgggcg cgacccgctc gccgtcgagt
     6181 cgctcgcccg cagcctgcag gactatctgg gctatcgcag ctcgggcgtg gaaacgcgcg
     6241 cgctctcggc gctcgatctc gcgctgtggg atctgttcgg caagtcctgc aacctgcccg
     6301 tctaccagat gctcggcggc gccagccgtc aatccgtgcg cacctacaac acctgcgccg
     6361 gctaccgcta cgcgcgcgac acgcgcggcc aggtgtcggc caactggggg ctcgacggaa
     6421 aaagcgaggg gccctacgaa gacctggacg ccttcctgca ccgcgccgac gagctcgcgc
     6481 attcgctgct cgagcaaggc atcaccgcga tgaagatctg gccgttcgac acctacgcgg
     6541 aagcgtcgga cggctggcac atatccggtt ccgatttaca ggaatcattg gaacccttcc
     6601 ggcggatccg taaagccgtc ggcgacaaga tggacatcat ggtggaattt cactcgctgt
     6661 ggcgcctgcc ggcggcgcag agaatcgcgc gcgcgctcgc cgagttcgac acgtactggc
     6721 acgaagaccc gatccgcatg gactcgctcg acctgctcaa gcagtacgcg gcgcattcca
     6781 aggcgccgat ctgcggctcc gagatcctcg ccgggcgctg gagcttcaag gacttcctcg
     6841 aaaccggcgt ggcgggcatc gtgatgttcg acctcggctg gtgcggcggc ctcaccgaag
     6901 cgcgccgcat cgccgcgctc gccgatgcct ggcagctgcc cgtcgcgccg cacgattgcg
     6961 tcggccctgt cgtcttcgcc gcctcgctcc acttctcgat ctgggcgccg aacgcgctgg
     7021 tgcaggaatc ggtgcgcgcc ttctacaccg gctggtacaa ggagctggtg atgaacgtgc
     7081 cgcagccggt tcgtggtttc tttgaaaaac cttccggtcc cggcctcggc tgcgaactgc
     7141 ttcccgaggt ctggaagcgc aaggacgcgg tggtgcgcgt cagtccagcc tgaccgcgat
     7201 tccttcgagc gccttcgacg gcgccgggta gcgctgcatc accagcggat cgtgtcccgg
     7261 aatgatgtgc tgcggcgact cggcgagcga gcgcagcttg ccgtagccgt cgaccatgtc
     7321 ggccaccgac cagacgatcg ggaacggccg cacctgctcc atgttggcgt agaaatggct
     7381 cgcgtcggcg gcgagcacca cccagccgcg gcgcgtcgcg acgcgtacca cctgcaggcc
     7441 catcgtgtgc ccgccgatga ggtgcagcga gacgccgggg gagagctccg catcgccgtc
     7501 gtggaagcgc acacggccgg catagacctc gcgcaccatg cccacgacgt cctccacctc
     7561 gaacgcgccg cggaagcagt cctggcacat atagcggccg gtggcgtagc gcatctccag
     7621 ctcctgcaga tgcagcgtcg cggcaggaaa gagatcgaag ttgccgacat ggtcgtagtg
     7681 cagatgggtg atcaccacgt ggttgatttt tttcgaatct actccaagca attccaaacc
     7741 ttgcgtcggg caccgaaggt ggtcgcgtcc gcgcttcgcc gccatcgccg ccgagaagcc
     7801 gg
//
LOCUS       DASYJA010000564         5145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1992036, whole genome shotgun sequence.
ACCESSION   DASYJA010000564 DASYJA010000000
VERSION     DASYJA010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1992036"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VGO02_12340"
     CDS             complement(<1..276)
                     /locus_tag="VGO02_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478513.1"
                     /translation="MLRLFEGRFGRLVLSELGPQDELHAQNDPAIVMQQGAAEILFLN
                     AGEIHVNLDTSRVLALHAAGEWLRESFPAAFSPADPRPFPQRREPVTL"
     gene            complement(301..771)
                     /locus_tag="VGO02_12345"
     CDS             complement(301..771)
                     /locus_tag="VGO02_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV7478514.1"
                     /translation="MDDINSLRRILRDCRVLAVVGLSADWYRPSYFAAKYMQEHGYRV
                     IPVNPKYDSILGERCYKSLRDIPEKVDLVDIFRKTQDVMPIAEDAIAIGARVLWQQLG
                     VKNEAAAAKARAAGLEAVMDRCVKIEHGRLFGGLNWVGVNTKVISAKRPRWLAY"
     gene            833..1174
                     /locus_tag="VGO02_12350"
     CDS             833..1174
                     /locus_tag="VGO02_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEV7478515.1"
                     /translation="MYVEERIYRLKIGAVAEYCRNYEELGMKTQLKHLPHMVGYYFTE
                     IGGLNCMVHQWAYDSLDQREKCRAAMQADPDWKKYLEKSRPLMESQENRIMKAAPFFV
                     ERLKIMLAAVK"
     gene            1171..2094
                     /locus_tag="VGO02_12355"
     CDS             1171..2094
                     /locus_tag="VGO02_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7478516.1"
                     /translation="MKPHPQVEKLLEAASRSPLPLYHTVPAFVARRIYHDTRGALAPK
                     RPEVAQVRLLVTPDKVALRSYRPIVDEVLPALVFFHGGGWTIGDLDTHDVLCRQLAAG
                     ARCAVFSVDYRLGPESPFPAAVEDSVSSTDYVFQNAEKLQIDPARIAVGGDSAGGNLA
                     AVVSIALKEKVAFQLLIYPATDQRGVHPSHERNGRGYLLTTDSMNYFRGSYLPRQADW
                     TDWRASPLLAPSLKGVAPAFVLTAGFDPLQDEGREYAERLKREGVQAEHRDYPDMIHG
                     FIVMGGVLDTANAAVADCCAALRRAFEKVTA"
     gene            2091..3758
                     /locus_tag="VGO02_12360"
     CDS             2091..3758
                     /locus_tag="VGO02_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEV7478517.1"
                     /translation="MKSDLKTRHGGKILTDALVAQGVKMAFGVPGESYLPVLDGLYDV
                     RDKLKFVVCRQEGGASYMAEAYGKLTGEPGVLMVTRGPGATNGAIGVHTGFQDSTPMV
                     VFVGQVGNEFVEREAFQEIDYRRMYGQMTKWVAQIDRVERIPEYVSRAFHTAMSGRPG
                     PVLLALPEDTLFAQAAVADVPRHHVVRPSPSQADMQEVKKLLEKSARPFVLIGGGGWN
                     REATRKLQKWAEAAGLPAGTSFRCQDLFDSRSPSYAGDVGIGINPKLAQRVKEADVVL
                     VIGARLGEMTTSAYSLLEAPVPSQTLIHVHAGAEELGRVYRPALAINSGYPQFVEALE
                     KISLKKTTTTAHDEYLAWSEPQPMPGKLQYGQVIRWLSDKLPEDAIIAGGAGNFAGWL
                     HRHFRYKGFRTQLGSTNGSMGYGYPAAVAAKLAAPSRTVLGLCGDGDFLMNGQEIATA
                     VQYGAAFVALVVNNGMYGTIRMHQEREYPGRVSGTDLQNPDFAAYARAFGGHGETVER
                     TEDFAPAYERAAASGKPALIELRIDPDAITPASTLSAIREKAIKSGR"
     gene            complement(3742..4104)
                     /locus_tag="VGO02_12365"
     CDS             complement(3742..4104)
                     /locus_tag="VGO02_12365"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEV7478518.1"
                     /translation="MTKRYSCLKCPGYCCSYPEIEVTPRDVERLAKHFGISYKETEER
                     YTKYDPKEKVQLLRHRKDTVFESTCMLFDQEKRRCTVYESRPAVCRGYPDSNRCGYYE
                     FLKFERAHQDDKQFIALT"
     gene            4218..4973
                     /locus_tag="VGO02_12370"
     CDS             4218..4973
                     /locus_tag="VGO02_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478519.1"
                     /translation="MKLFKGVIAAVAIATTTPAAAVGSLVDLTVYDRTEGKRLQVYWH
                     QGRAYVVGRPGNEYQVSLRNRAGDDVLAIVSVDGVNVITGQSASPQQSGYVLAPRGGM
                     DIQGWRKSMAETAAFYFTPLSDSYAARTGRPDDVGVIGLALFRKKYAPPPASVGQPFP
                     GPYSERESAAGARSSQDAAKPAEPQLGTGHGRRESSHAQYVSFERATSAPAEVISLYY
                     DSHRNLVARGVIRDTVPVAPLSRPFPGFVPDPA"
BASE COUNT          897 a         1682 c         1719 g          847 t
ORIGIN      
        1 cagcgtgacc ggctcgcggc gctgcgggaa cgggcgcggg tcggcggggg agaacgcggc
       61 cgggaagctc tcgcgcagcc attcgccggc ggcgtgcagc gcgagcacgc gcgaggtgtc
      121 gaggttgacg tggatctcgc ccgcgttgag gaacaggatc tcggccgcgc cctgttgcat
      181 gacgatggcc gggtcgttct gggcgtgcag ctcgtcctgc ggcccgagct cgctcaggac
      241 gagccgcccg aacctgcctt cgaatagtct gagcatcctc cccatggcgg aaaatatacc
      301 tcagtaggcg agccagcggg ggcgttttgc ggagattacc ttcgtgttga ccccgaccca
      361 gttcagaccc ccaaaaagcc ttccgtgctc gatctttacg cagcggtcca tgacggcttc
      421 cagcccggcg gcgcgggcct tggcggccgc ggcctcgttt ttgaccccca gctgctgcca
      481 gagcacccgg gcgccgatgg cgatggcatc ctccgcaatc ggcataacgt cctgggtctt
      541 tcggaatatg tccaccaggt cgaccttctc cgggatgtcg cgcagggact tgtagcagcg
      601 ttcgccgagg atgctgtcgt acttcgggtt gaccgggatc acccggtagc cgtgctcctg
      661 catgtatttc gcggcgaaat agctcgggcg gtaccagtcg gccgagaggc ccaccaccgc
      721 gagcacgcgg cagtcgcgca ggatgcggcg cagtgaattg atgtcgtcca tcagtggatg
      781 tcgtccatga cgcagatact ataatcgaag caacccctta ggagagccag gcatgtacgt
      841 ggaagagcgc atctaccgcc tgaaaatcgg cgcggtggcc gagtactgca ggaactacga
      901 agagcttggc atgaagacgc agttgaagca cctgccgcac atggtcggct actacttcac
      961 cgagatcggc ggcctcaact gcatggtcca ccagtgggcc tacgacagcc tcgaccagcg
     1021 cgagaagtgc cgcgccgcca tgcaggccga cccggactgg aaaaagtatc tggagaagtc
     1081 gcgcccgctg atggagtcgc aggagaaccg gatcatgaag gcggcccctt tcttcgtcga
     1141 gcggctgaag ataatgctcg cagcggtcaa gtgaagccgc atccacaggt cgagaagctg
     1201 ctggaggcgg cgagccgctc gccgctgccg ctgtaccaca cggtgcccgc gttcgtggcc
     1261 cgccgcatct accacgacac gcgcggcgcg ctggcgccga agcggcccga ggtggcgcaa
     1321 gtccggctgc tcgtcacgcc cgacaaggtt gcgctgcgca gctaccgccc catcgtcgac
     1381 gaggtgctgc cggcgctggt gttcttccac ggcggcggct ggaccatcgg tgatctcgac
     1441 acgcacgacg tgctgtgccg ccagctcgcg gccggcgcgc gctgcgcggt gttttccgtc
     1501 gactaccgcc ttggaccgga gagcccgttt cctgccgcgg tggaagacag cgtttcttct
     1561 accgactatg tttttcaaaa tgccgaaaag ctgcagatag atccggcccg catcgccgta
     1621 ggcggcgaca gcgcgggagg caacctcgcg gcggtggttt cgatcgcact gaaggagaag
     1681 gttgcgttcc agcttctcat ctatcccgcc accgaccagc gcggcgtgca tccctcgcac
     1741 gagcgaaacg ggcgcggcta cctgctgacc acggattcga tgaattactt ccgcggcagt
     1801 tacctgcccc ggcaggcgga ctggaccgac tggcgcgcct cgccgcttct cgcgcccagc
     1861 ctgaaaggcg tggcgccggc gttcgtgctc accgcgggct tcgacccgct gcaggacgaa
     1921 gggcgcgagt atgcggagcg ccttaagcgc gagggcgtgc aggccgagca ccgcgactat
     1981 cccgacatga tccatggctt catcgtgatg ggcggcgtgc tcgacacggc caacgccgcc
     2041 gtcgcggact gctgcgcggc attgcgccgc gcctttgaga aagtcactgc atgaaatcgg
     2101 acctgaagac tcgtcacggc ggcaagatcc tgaccgacgc gctggtggcg cagggcgtga
     2161 agatggcctt cggcgtgccc ggcgaaagct acctgccggt gctcgacggc ctgtacgacg
     2221 tgcgcgacaa gctcaagttc gtcgtctgcc gccaggaagg cggcgcctcc tacatggccg
     2281 aggcctacgg caagctgacc ggcgaacccg gcgtgctgat ggtgacgcgc ggccccggcg
     2341 cgaccaacgg cgccatcggc gtgcacaccg gcttccagga ttccacgccg atggtcgtgt
     2401 tcgtcgggca ggtcggcaac gaattcgtcg agcgcgaagc gttccaggaa atcgactacc
     2461 ggcgcatgta cggccagatg acgaagtggg tggcgcagat cgaccgcgtc gagcgcatcc
     2521 ccgagtacgt cagccgcgcg ttccacaccg cgatgtcggg ccggcccggg ccggtgctgc
     2581 tcgcgctgcc tgaggacacg ttgtttgcgc aagccgccgt ggcggatgtg ccgcggcatc
     2641 acgtcgttcg tccatcgcct tcgcaggcgg acatgcagga ggtaaaaaaa ctcctggaaa
     2701 aatccgcgcg ccccttcgtc ctgatcggcg ggggcggctg gaaccgcgag gcgacgcgga
     2761 agctgcagaa gtgggccgag gcggcgggcc tgccggcagg tacctccttc cgctgccagg
     2821 acctgttcga cagccgcagc ccgagctacg ccggcgacgt cggcatcggc atcaatccca
     2881 agctcgcgca gcgcgtgaag gaggccgacg tcgtgctggt gatcggcgcg cggctgggcg
     2941 agatgaccac cagcgcctac agcctgctcg aggcgccggt gccgagccag acgctgatcc
     3001 acgtgcacgc gggagcggaa gagctgggcc gcgtttaccg tcctgcgttg gccatcaatt
     3061 caggctatcc gcaattcgtc gaagcgcttg aaaaaatctc gttgaagaaa acaaccacca
     3121 ccgctcacga tgagtatctc gcgtggagcg agccgcagcc catgccgggc aagctgcagt
     3181 acggccaggt gatccgctgg ctgagcgaca agctgcccga ggacgcgatc atcgccggcg
     3241 gcgcggggaa tttcgccggc tggctgcacc ggcacttccg ctacaagggc ttccgcacgc
     3301 agctcggctc gaccaacggg tccatgggtt acggctaccc ggcggcagtg gcggccaagc
     3361 tagccgcgcc cagccgaacg gtcctcggcc tgtgcggcga cggcgacttc ctgatgaacg
     3421 gccaggagat cgccaccgcc gtgcagtacg gcgcggcgtt cgtcgcgctg gtggtgaaca
     3481 acggcatgta cggcacgatc cgcatgcacc aggagcgcga gtacccgggc cgcgtctcgg
     3541 ggaccgacct gcagaacccg gatttcgccg cctacgcccg cgccttcggc gggcacggcg
     3601 agacggtgga gcggaccgag gacttcgcgc cggcctacga gcgcgccgcc gcttcgggca
     3661 agccggcctt gatcgagctg cgcatcgacc cggacgccat tacaccggcc tccaccctct
     3721 ccgctatccg cgagaaggca atcaagtcag ggcgatgaat tgcttgtcgt cctggtgggc
     3781 gcgctcgaac tttaggaact cgtagtagcc gcagcggttg ctgtcggggt agccgcggca
     3841 gacagccggc cgcgactcgt acacggtgca gcggcgcttc tcctggtcga acagcatgca
     3901 ggtcgactcg aacaccgtgt ccttgcggtg gcgcaggagc tgcaccttct ccttggggtc
     3961 gtacttcgtg tagcgctcct cggtctcctt gtagctgatg ccgaagtgct tggccaggcg
     4021 ctcgacgtcg cgcggtgtga cttcgatctc ggggtagctg cagcagtagc ctggacattt
     4081 gaggcaggaa tagcgcttcg tcattcgggg agtgcctttc gtgaagcggc gcattctacg
     4141 cgagccatgt gaattttctt tcgtttaacc ctttcgcgcc gcgctgccgt ttcactcggc
     4201 agaaaggaga atcacccatg aagctcttca agggagtcat cgctgcagta gcaatagcaa
     4261 caactacgcc cgcagccgcg gtgggtagtc tcgtggacct gaccgtgtac gaccgcaccg
     4321 aaggaaagcg cctgcaggtg tattggcacc aggggcgtgc gtacgtggtg ggcagacccg
     4381 gcaacgagta ccaggtgagc ctgcgcaacc gcgccggcga cgatgtgctg gcgatcgtct
     4441 cggtcgacgg cgtcaacgtc atcaccgggc agagcgcctc gccccagcaa agcggctatg
     4501 tcctcgcgcc gcgtgggggc atggacatcc agggctggcg caagagcatg gccgagacgg
     4561 cggcgttcta cttcaccccg ctgtccgatt cctacgcggc acgcaccggg cgcccagacg
     4621 acgtcggagt gatcgggtta gctctcttca ggaagaaata cgcgccacca ccggcgagcg
     4681 tgggacagcc gtttcccggg ccgtattccg aaagggaaag cgctgccggc gcccgctcca
     4741 gccaggatgc ggccaagccc gccgagccgc agctcggcac cgggcacggg cggcgcgaga
     4801 gctcccacgc gcagtacgtg agcttcgagc gcgcgacgag cgcgccggcc gaggtgatct
     4861 cgctctacta cgacagccat cgcaacctgg tggcgcgggg cgtgatccgc gacaccgtgc
     4921 ccgtggcgcc gctgtcgcgc ccgttccccg gcttcgttcc cgacccggcg tagactcatg
     4981 cgtagtggag gccccggacg aacaactcat gctcgcgtac cgagacggga acgccggggc
     5041 cttcgaaacg ctctatacaa ggcatcgcgg ccggctttac cgcttcgtgc tgcgcagcgt
     5101 gaaagccagc ggcacggccg aggagctgtt ccaggaagtc tggat
//
LOCUS       DASYJA010000565         2697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3174268, whole genome shotgun sequence.
ACCESSION   DASYJA010000565 DASYJA010000000
VERSION     DASYJA010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3174268"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..773)
                     /locus_tag="VGO02_12375"
     CDS             complement(60..773)
                     /locus_tag="VGO02_12375"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7478520.1"
                     /translation="MAQKAQKGTVLVTGGARGIGQATASLLAARGYRVAVADLNAPAG
                     RGRTFFFRRTDVSSEPAVRACVRAVLERFGRLDALVNNAGIADPANGPLEKLSLAEWN
                     RRIGTNLTGAFLMAKHCAPHLRAARGAIVSIASTRAVQSEPQTEAYTAAKGGLVALTH
                     ALAMSLGPRVRVNCISPGWIAHGRVRRKDHAQHPVGRVGRDDDVAELVGFLLSDAAGF
                     ATAQNFVLDGGMTRKMIYV"
     gene            813..1247
                     /locus_tag="VGO02_12380"
     CDS             813..1247
                     /locus_tag="VGO02_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV7478521.1"
                     /translation="MQVKEILRVKGSRLLSIDPAGRAAEAVATMAKENLGSLVVMEQG
                     RMVGMLTFHELLRALAGRNGSLGDLKVSDIMERDPVTAAPEMEVNDLRRTMLESGARY
                     LPVMQNDKLLGVISFRDVAKAVLEEQDFENKMLKGYIKNWPA"
     gene            1255..2478
                     /locus_tag="VGO02_12385"
     CDS             1255..2478
                     /locus_tag="VGO02_12385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEV7478522.1"
                     /translation="MKRRGFLYAAGVLAGAGALAAWRFWPEQGLWNPCLARLPRRLAE
                     HDLVRAAWQGLDASRVWDSHAHLIGTGDSGSGIVVNPATDSLLSPAQYARRLFFLNAG
                     CAHEAAGSVDRAYVERMRNLLDGMPAGVKLVLYAFERAHDERGAPDWEHSTFYVPDAY
                     ARDVAKAYPASFEWVASIHPYRPDALDALAWAKKEGARGVKWLPSAMGIDPASPRCDP
                     FYLALEKNGMPLIAHAGLERAVLGREMHDYGNPLRLRRALDAGVRVVVAHCASMGEDR
                     DLDKGENGPYVDSFSLFTRIFETYAQHCYGDISAMTQVNRAGPLLAKVIERSDWHARL
                     LNGSDYPLPGVMPIFSVDYLVSLGLLKEGLAPILKEIRLHNPLLFDFVLKRHLQSNNK
                     MLSASVFETRMFFMR"
     gene            2486..>2697
                     /locus_tag="VGO02_12390"
     CDS             2486..>2697
                     /locus_tag="VGO02_12390"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV7478523.1"
                     /translation="MSGNTFGTLYCVTSFGESHGPAYGGVVDGCPPGLELSAQDIQVE
                     LDRRKPGSSRHVTQRRETDTVEILSGV"
BASE COUNT          444 a          878 c          921 g          454 t
ORIGIN      
        1 accgcgaccg acgtggtgag catgggaatg ctgttggggc tggccacgct gctcgtgcat
       61 tagacataga tcatcttgcg ggtcatgccg ccgtccagca cgaagttctg agcggtagcg
      121 aagccggccg catccgagag caggaagccg accagctcgg ccacgtcgtc gtcgcgcccg
      181 acccggccga ccggatgctg ggcgtggtcc ttgcgccgca cgcggccgtg cgcgatccag
      241 cccgggctga tgcagttcac gcgcacgcgc ggcccgaggc tcatcgccag cgcgtgcgtc
      301 agcgcgacca ggccgccctt ggccgcggtg taggcctcgg tctgcggctc cgattgcacg
      361 gcgcgcgtgg acgcgatgct cacgatcgcg ccgcgcgctg cgcgcaggtg cggcgcgcaa
      421 tgcttggcca tcaggaaggc cccggtgagg ttggtgccga tgcgccggtt ccactcggcg
      481 agggagagct tctcgagcgg gccattcgcc gggtcggcga tgccggcgtt gttgaccagg
      541 gcatccaggc ggccgaagcg ctcgagcacc gcgcgcacgc aggcccgcac ggccggctcg
      601 ctcgacacgt cggtccggcg gaagaaaaac gtccggccgc gccccgccgg ggcgttgagg
      661 tcggccacgg cgacccggta gccgcgcgcg gcaaggagcg atgcggtcgc ctggccgata
      721 ccccgcgcgc cgccggtcac gaggactgtt cccttttgtg ccttttgcgc catagccgaa
      781 aagagtattc tccgaacaga cggaggtcca ccatgcaagt gaaggaaatc ctgcgcgtca
      841 agggtagccg cttgctcagc atcgatccgg ccggcagggc cgccgaagcg gtcgcgacga
      901 tggcgaagga gaacctcggc tcgctggtgg tgatggagca ggggcgcatg gtgggcatgc
      961 tcaccttcca tgagcttctg cgcgcactcg ccgggcgcaa cggatcgctg ggtgatctga
     1021 aggtgtccga catcatggag cgcgacccgg tgaccgcggc gccggagatg gaagtcaacg
     1081 acctgcgccg caccatgctg gaaagcggcg cgcgctacct gccggtgatg cagaacgaca
     1141 agctgctcgg cgtgatctcc tttcgcgacg tggcgaaggc cgtgctcgaa gagcaggact
     1201 tcgagaacaa gatgctgaag ggctacatca agaactggcc tgcctgaagg ttttttgaaa
     1261 agacgcggtt ttctctacgc cgccggagtc ctggccggcg cgggcgcgct tgccgcctgg
     1321 cgtttctggc cggagcaggg gttgtggaac ccctgcctcg cgcgcctgcc gcgccggctc
     1381 gccgagcacg acctggtgcg cgccgcatgg caggggctcg acgcgtcgcg ggtctgggac
     1441 agccacgccc acctcatcgg caccggcgac tcgggcagcg gcatcgtggt caacccggcg
     1501 accgacagcc ttctcagccc ggcgcagtac gcgcggcgcc tgttcttcct caacgcgggc
     1561 tgcgcgcatg aggcggcggg tagcgtcgac cgcgcctacg tcgagcgcat gcgcaacctc
     1621 ctcgacggca tgccggccgg cgtcaagctg gtgctctatg cattcgagcg cgcgcacgac
     1681 gagcgcggcg cgcccgactg ggagcacagc accttctacg tgccggatgc ctacgcgcgc
     1741 gacgtcgcca aggcctaccc ggcgagcttc gaatgggtgg cttcgattca tccctatcgg
     1801 ccggatgcgc tcgacgcgct tgcctgggcg aagaaggaag gcgcgcgcgg ggtgaagtgg
     1861 ctgccgtcgg cgatgggaat cgatccggct tcgccgcgct gcgatccgtt ctaccttgcg
     1921 ctggaaaaaa atggcatgcc gctgatcgcg cacgccgggc tggagcgcgc ggtgctcggc
     1981 cgcgagatgc acgactacgg caatccgctt cgcttacgac gtgcgttgga cgccggcgtg
     2041 cgcgtggtgg tcgcgcactg cgcttccatg ggcgaggacc gcgatctgga caaaggggaa
     2101 aacggcccgt acgtcgacag cttttcgctt ttcacaagaa tattcgagac ctatgcgcag
     2161 cactgctacg gcgacatctc ggcgatgacg caggtcaacc gtgccggccc gttgctcgcg
     2221 aaagtcatcg agcgctccga ctggcacgcg cgcctgctca acggctcgga ctatccgctg
     2281 ccgggcgtga tgccgatctt ctcggtggac tacctcgtgt cgctcggtct gctgaaggag
     2341 ggcctggctc ccatcctgaa ggagattcga cttcacaacc cgctgctctt cgatttcgtg
     2401 ctgaagcggc atctgcaatc gaacaacaag atgctctcgg cgagcgtttt cgagacgcgg
     2461 atgtttttca tgagatgatt tccgcatgtc gggaaacact ttcggcacgc tctactgcgt
     2521 cacctcgttc ggtgaatcgc atggtccagc ctatggcggg gtggtcgacg gctgtccacc
     2581 cggcctggaa ctttccgcgc aggatatcca ggttgaactg gatagaagaa aaccaggcag
     2641 ttcccgccac gtcacccagc gccgcgagac cgacacggtc gagatcctct ccggggt
//
LOCUS       DASYJA010000566         6816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1720012, whole genome shotgun sequence.
ACCESSION   DASYJA010000566 DASYJA010000000
VERSION     DASYJA010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6816
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1720012"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /gene="rph"
                     /locus_tag="VGO02_12395"
     CDS             complement(<1..365)
                     /gene="rph"
                     /locus_tag="VGO02_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006496577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEV7478524.1"
                     /translation="MSAEVITPRPSGRKPDQLRAVRITRAYTRNPEGSVLVEFGDTRV
                     LCTASVEERVPPFLKESGRGWVTAEYGMLPRATNTRGDREAARGKQSGRTQEIQRLIG
                     RALRAVVDLSALGQRTVHL"
     gene            complement(362..1279)
                     /locus_tag="VGO02_12400"
     CDS             complement(362..1279)
                     /locus_tag="VGO02_12400"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEV7478525.1"
                     /translation="MKYAIVQDSRLGARRINQDRIGYWRTPEALLAVVADGMGGHLHG
                     EVAANLAADLFGTAFQREARPRLANPAEFLAQAVVLAHTQILAEADKRGLAESPRTVI
                     VACVVQDGQACWMHVGDCRLYLFRKGRVMARTRDHTRVQQLVDEGRIREEAIVSHPER
                     NRLLQCLGGIQPPRPDVASKTALEKDDIVLLCSDGFWGPLTQRQMLNALLARELKEAV
                     PELVALAEARAGQECDNVSVLAINWGEEMRAPLAAQGPNTLPYYELPTDVQDLTATDP
                     DFLRVSDEDIDNAIAEIKAALRKNVPPRR"
     gene            complement(1276..2214)
                     /locus_tag="VGO02_12405"
     CDS             complement(1276..2214)
                     /locus_tag="VGO02_12405"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV7478526.1"
                     /translation="MPSQANQPLPEGTPLENYRIMRVLATGGFSFVYLAHDENDAPVA
                     IKEYLPSTLAVRVNGAAYPSIPEADLAKFRYGMKCFFEEGRALAGLSHPNVVRVLNFF
                     RANETVYLVMRYERGRTLQEHIRSRRGALEEEWIRKTFTHLLNGLREVHSNKLLHLDI
                     KPGNVYVRNDGTPLLIDFGAARQTLSAEGMKLPPTYTPGFASPEQYARRELLGPWSDI
                     YSVGATMYACFAAAAPQPADERLEKDLVVPARRNWAGKYSADLLDIVDWCLRMDHMER
                     PQSVFALQKTLTGEKAPEHRGKAPLLDRMRGMLRRG"
     gene            2282..3136
                     /locus_tag="VGO02_12410"
     CDS             2282..3136
                     /locus_tag="VGO02_12410"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010809226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEV7478527.1"
                     /translation="MTGYAAASAESPRGRLSLELRTVNARFLDLQFRVAEELRALEPL
                     LREAIAARVSRGKVDCRFYFNESAGAQVAQRLDPAALARLRVLSEEAKKAFPEAGALR
                     VADVLRWPGVTAEPPLDEEGLRIAAHDLCRRALDELVAARGREGAKLAASIGERVAAM
                     RRRVDDVKPLVPQAIVAYQAKLAERLKEALGSADDERIRTEIAVFAGKSDVDEELERL
                     TVHLAEVDRVLKHGGAAGKRLDFIAQELNREANTLASKAASAPIADCALELKLLIEQV
                     REQVQNIE"
     gene            3129..3719
                     /gene="gmk"
                     /locus_tag="VGO02_12415"
     CDS             3129..3719
                     /gene="gmk"
                     /locus_tag="VGO02_12415"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016473713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV7478528.1"
                     /translation="MSSGKGKLFVITAPSGAGKSSLIQALLKEDPSLRLSTSYTTRPP
                     RPGEQNGREYHFVKDSEFLAMRSRGEFLESAEVHGNRYGTAKRVIEEALARGHDLLLE
                     IDWQGARQVRRLHPDCVGIFILPPSVEELERRMRTRGQDADEVIQRRLANARDELTHV
                     DEFKYRIINKDFEAAKGELSEIIRKERAKPHGANHR"
     gene            3700..3927
                     /gene="rpoZ"
                     /locus_tag="VGO02_12420"
     CDS             3700..3927
                     /gene="rpoZ"
                     /locus_tag="VGO02_12420"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEV7478529.1"
                     /translation="MARITVDDCLKRIPNRFHLTLAATYRARQLGSGASPLVEPNRDK
                     ATVIALREIASGKVGTEVLKKNPTSSTLPTL"
     gene            3958..6069
                     /locus_tag="VGO02_12425"
     CDS             3958..6069
                     /locus_tag="VGO02_12425"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEV7478530.1"
                     /translation="MAAVPTLEQGRFGYLKPKDVARLAEAYRFSEAAHSGQTRQSGDP
                     YISHPLAVAEILADWRLDGQTLMAALLHDVTEDTKVTKDEISDTFGRPVADLVDGVSK
                     LDKIEFQSAEVAQAENFRKMLLAMARDVRIILIKLADRLHNMRTLGAVPPAKRRRVAR
                     ETMEIYAPIANRLGLNTLFHELQELAFSHLYPGRYKVLARATAAARGNRREMIGRTLE
                     AIKKKLAEAGLKATVQGREKHVYSTYRKMIEKHLSFSEVHDIFGCRVVVEDVPACYLA
                     LGTLHALYKPIPGKFKDYIAIPKANGYQSIHTDLIGPYGVPVEVQIRTEQMHRLAESG
                     VASHWMYKDEAATLSDLQKKTHQWLQSLLEIQNQSGDSAEFLEHIKVDLFPDEVYVFT
                     PKGRILSLPRGATAVDMAYAVHTDIGNRCVAAKVNGELVPLRTDLRNGDRVEIITASH
                     AKPNPGWLQYVRTGKARSNIRHFLKTMQYEESASLGERLLEQALKSLKGSVAEIDDAG
                     WDRALRDSGARSRGDLLADIGLGKRLPAVVARRLLKRSDSKEELKSAGSVTIRGTEGM
                     AVQLASCCRPIPGDAIVGSMKKGQGLVVHASECTVIERSRKKEPEQWIDVEWDPRTSR
                     LFQAAINVTVENQRGVLAKVASEIASAASNIDAISMDEDRALFTTMHLVVEVANRQHL
                     ARVLRALRRIPDVKKISRMRE"
     gene            6169..6804
                     /locus_tag="VGO02_12430"
     CDS             6169..6804
                     /locus_tag="VGO02_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008453082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV7478531.1"
                     /translation="MISARACVSVIDDDALVLRSLERLLQSRGFEVHAYSSPQAFLEQ
                     RDADSPGCIVMDLSMPGLSGLELQQALAHAADRRPVVFISGRSSVEASVRAMKAGAVD
                     FLTKPFDEADLVAAVYAAVEKDRASREGLAERASINLRLSTLTPRERDVLSRVVAGKL
                     NKQIAAELGTAEKTVKVHRARMMRKMQFDSLAELVRAWTVTQPTPPAPPQI"
BASE COUNT         1143 a         2329 c         2296 g         1048 t
ORIGIN      
        1 tcgagatgca ccgtgcgctg gccgagcgcg gaaagatcga ccaccgcgcg cagcgcccgg
       61 ccgatcaggc gctggatctc ctgcgtgcgg cccgactgct tgccgcgcgc cgcctcgcgg
      121 tcgccgcgcg tgttggtcgc gcgcggcagc atgccgtact ccgccgtcac ccagccgcgg
      181 ccgctctcct tgagaaacgg cggcacgcgc tcctcgacgc tcgcggtgca cagcacgcgc
      241 gtgtcgccga actcgaccag caccgagccc tccggattgc gcgtgtaggc gcgggtgatg
      301 cgcaccgcgc gcagctggtc gggcttgcgg ccgctcggcc gcggggtgat gacttcggcg
      361 ctcatcgccg cggcggcacg ttcttgcgca gcgccgcctt gatctcggcg atcgcgttgt
      421 cgatgtcctc gtcggacacg cgcaggaaat ccgggtcggt ggcggtcagg tcctgcacgt
      481 cggtcggcag ctcgtagtag ggcagcgtgt tcggcccctg cgcggctagc ggggccctca
      541 tctcctcgcc ccagttgatg gcgaggaccg agacattgtc gcattcctgc ccggcgcgcg
      601 cctcggccag cgcgaccagc tcggggaccg cttccttcag ctcgcgcgcg agcagcgcgt
      661 tgagcatctg gcgctgcgtg agcggccccc agaagccgtc ggagcaaagc agcacgatgt
      721 cgtccttctc cagcgccgtt ttcgaagcga cgtccggtcg cggcggctgg atgccgccca
      781 ggcactgcag cagccggttg cgctcggggt gcgacacgat cgcctcctcg cggatgcggc
      841 cctcgtcgac gagctgctgc acgcgcgtgt ggtcgcgcgt gcgcgccatg acgcggccct
      901 tgcgaaacag gtacagccgg cagtcgccga cgtgcatcca gcaggcctgg ccgtcctgca
      961 ccacgcaggc cactatcacg gtgcgcggcg actcggccag gccgcgcttg tcggcctcgg
     1021 ccaggatttg cgtgtgggcg aggaccacgg cctgcgccag gaactcggcg ggattggcga
     1081 gccgcggcct ggcctcgcgc tggaacgccg tgccgaacag gtcggcggcc aagttggcgg
     1141 cgacctcgcc gtgcaggtgc ccgcccatgc cgtcggccac caccgcgagc agcgcttcgg
     1201 gcgtgcgcca gtagccgatg cgatcctggt tgatgcggcg cgcgccgagg cggctgtcct
     1261 gcacgatggc gtacttcatc cgcgcctcag catgccgcgc atgcggtcga ggagcggcgc
     1321 cttgccgcgg tgctcgggcg ctttctcgcc ggtgagcgtc ttctgcagcg cgaacacgct
     1381 ctgcggccgc tccatgtggt ccatgcgcag gcaccagtcg acgatatcga ggaggtccgc
     1441 cgagtatttg ccggcccagt tgcgccgcgc cggcaccacc aggtccttct cgaggcgctc
     1501 gtcggccggc tgcggcgccg ccgcggcgaa gcacgcgtac atggtcgcgc cgacggagta
     1561 gatgtcgctc cacgggccca gcagctcgcg gcgcgcgtat tgctcgggcg aagcgaagcc
     1621 cggtgtatag gtgggcggca gcttcatgcc ctcggcggag agcgtctggc gcgcggcgcc
     1681 aaagtcgatc agaagcggag ttccatcgtt gcgcacgtag acattgccgg gcttgatgtc
     1741 gaggtgcagc agcttgttcg agtgcacctc gcgcagcccg ttcagcagat gggtgaaggt
     1801 cttgcggatc cactcttcct cgagcgcgcc gcggcggctc ctgatgtgct cctgcagcgt
     1861 cctgccgcgc tcatagcgca tgaccaggta gaccgtctcg ttggcgcgga agaagttgag
     1921 cacgcgcacc acgttggggt gcgacaggcc ggcgagcgcg cgcccttcct cgaagaagca
     1981 cttcatgccg tagcggaact tggcgaggtc cgcctcgggg atgctcgggt aggcagcgcc
     2041 gttgacgcgc accgcgagcg tcgagggcag gtactccttg atcgccaccg gcgcgtcgtt
     2101 ctcgtcgtgc gcgagataga cgaacgagaa gcctccggtc gccagcaccc gcatgatgcg
     2161 gtagttctcg agcggggtgc cttcgggcag cggttggttg gcttgcgatg gcatctcccc
     2221 tatgatacgc gtccgctaat cgagcgcggc gcgcgcactt tttcacaaaa tgattcaaag
     2281 catgactggt tacgcggccg catccgccga atccccccgc ggccggctga gcctcgagct
     2341 gcgcacggtg aacgcgcgct tcctcgacct gcagttccgc gtcgccgagg agctgcgcgc
     2401 gctcgagccg ctgctgcgcg aggcgatagc cgcgcgcgtc tcgcgcggca aggtcgattg
     2461 ccgcttctac ttcaacgagt cggccggcgc gcaggttgcc cagcggctcg atccggcggc
     2521 gctcgcgcgc ctcagggtgc tgagcgagga agcgaagaag gctttccccg aagccggcgc
     2581 gctgcgcgtg gccgacgtgc tgcgctggcc cggtgtcacg gccgagccgc cgctcgacga
     2641 ggaagggctg cgcattgcgg cgcatgatct gtgccggcgc gcgctggacg agctggtcgc
     2701 ggcgcgcggc cgcgagggcg ccaagctcgc cgccagcatc ggcgagcgcg tcgccgcgat
     2761 gcgccgccga gtggacgatg tgaaacccct cgtaccgcaa gccatcgttg cgtaccaagc
     2821 gaagcttgcg gagcggctga aggaagcgct gggctccgcc gacgacgagc gcatccgtac
     2881 ggagatcgcc gtgttcgcgg gcaagagcga cgtcgacgag gaactggagc ggctgaccgt
     2941 tcaccttgcg gaagtggatc gggtgctgaa gcacggcggg gcggcgggta agcgcctgga
     3001 cttcatcgcc caggaactga accgcgaggc gaacacgctc gcctccaagg ccgccagcgc
     3061 gccgatcgcc gactgcgcgc tcgagctcaa gctgctcatc gagcaggtcc gcgagcaggt
     3121 gcagaacatt gagtagcggc aaaggcaagc tgttcgtcat caccgcgccg tcgggcgcgg
     3181 gcaagagctc cctcatccag gcgctcttga aggaagatcc gtcgctcagg ctgtcgactt
     3241 cgtacaccac gcggccgccg cggcccggcg agcagaacgg ccgcgaatac cacttcgtca
     3301 aggacagcga gttcctggcg atgcgcagcc gcggcgaatt cctggaaagc gccgaggtgc
     3361 acggcaaccg ctacggcacg gcgaagcggg tcatcgagga ggccctggcg cgcggccacg
     3421 acctgctgct cgagatcgac tggcagggcg cgcggcaggt gcgcaggctc cacccggact
     3481 gcgtcggcat ttttatcctg ccgccctccg tcgaggagct cgagcggcgc atgcgcaccc
     3541 gggggcagga tgccgacgaa gtcatccagc gccgcctggc gaacgcccgc gacgaattga
     3601 cccacgtcga cgagttcaaa taccgtataa ttaacaaaga cttcgaggcc gccaagggcg
     3661 agctgtcgga aatcattcga aaggaaagag caaaacccca tggcgcgaat caccgttgac
     3721 gattgcctga agcgaattcc caaccggttt cacctgacgc tggcggcgac ctaccgtgcc
     3781 cgccaactcg gctccggcgc gagcccgctg gtcgagccca accgcgacaa ggcgaccgtg
     3841 atcgcgctgc gcgagatcgc ctcgggcaag gtcggcaccg aagtgctgaa gaagaatccg
     3901 acgtcgtcga cgctgccgac gttgtaacgc cttcatcttg agaggtagtc tctcaagatg
     3961 gcagcagttc ctaccctcga gcaaggccgc ttcggctacc tgaagccgaa ggacgtggcg
     4021 cgcctcgccg aggcgtaccg cttctccgaa gccgcgcact ccggccagac gcgccagtcc
     4081 ggcgacccgt acatctccca cccgctcgcc gtcgccgaga tcctcgccga ctggcgcctc
     4141 gacgggcaga cgctgatggc cgcgctgctg cacgacgtga ccgaggacac caaggtcacc
     4201 aaggacgaga tctccgacac cttcggcagg ccggtcgccg acctggtcga cggcgtctcc
     4261 aagctcgaca agatcgagtt ccagtcggcc gaggtggcgc aggccgagaa cttccgcaag
     4321 atgctgctgg cgatggcgcg cgacgtgcgc atcatcctga tcaagctcgc cgaccgcctg
     4381 cacaacatgc gcaccctcgg cgccgtgccg ccggccaagc gccggcgggt cgcccgggaa
     4441 acgatggaga tctacgcgcc gatcgccaac cgcctcggcc tcaacacgct gttccacgag
     4501 ctgcaggagc tcgccttctc gcacctctat ccggggcgct acaaggtgct cgccagggcc
     4561 acggcggcgg cgcgcggcaa ccgccgcgag atgatcggca ggacgctcga ggcgatcaag
     4621 aaaaagctcg ccgaagccgg cctcaaggcc accgtgcagg gccgcgagaa gcacgtctat
     4681 tccacctacc gcaagatgat cgagaagcac ctctcgttct ccgaggtgca cgacatcttc
     4741 ggctgccgcg tggtggttga ggacgtgccc gcctgctacc tcgcgctcgg cacgctgcac
     4801 gcgctctaca agccgatccc gggcaagttc aaggactaca tcgccatccc gaaggcgaac
     4861 ggctaccagt cgatccacac cgatctgatc ggcccctatg gcgtgccggt cgaggtgcag
     4921 atccgcaccg agcagatgca ccgcctggcc gagtcgggcg tcgcctcgca ctggatgtac
     4981 aaggacgagg ccgccacgct gtcggacctg cagaagaaga cccaccagtg gctgcagtcg
     5041 ctgctcgaga tccagaacca gtcgggcgac tcggccgaat tcctcgagca catcaaggtc
     5101 gatctctttc cggacgaggt atacgtcttc acgccgaagg ggcgcatcct gtcgttgccg
     5161 cgcggcgcca ccgcggtgga tatggcctat gcggtgcaca ccgacatcgg caaccgctgc
     5221 gtggcggcga aggtgaacgg cgagctcgtc ccgctgcgca ccgacctgcg caacggcgac
     5281 cgcgtcgaga tcatcaccgc gagccatgcc aagcccaatc ccgggtggct gcagtacgtg
     5341 cgcaccggga aggcgcgctc gaacatccgc cacttcctga agaccatgca gtacgaggag
     5401 tcggcgagcc tgggcgagcg cctgctcgag caggcgctga aaagcctgaa gggctcggtc
     5461 gccgagatcg acgacgccgg ctgggaccgc gcgctgcgcg attccggcgc gcgctcgcgc
     5521 ggcgatctcc tcgccgacat cggcctcggt aagcgcctgc cggcggtggt cgcacgccgg
     5581 ctcctcaagc gcagcgattc gaaagaggag ctgaagagcg cgggcagcgt caccatccgc
     5641 ggcaccgagg gcatggcggt gcagctcgcc tcgtgctgcc gcccgatccc gggcgacgcc
     5701 atcgtcggct ccatgaagaa ggggcagggc ctggtggtgc acgcctccga atgcaccgtg
     5761 atcgagcgct cccgcaagaa agagcccgag cagtggatcg acgtcgagtg ggaccctcgc
     5821 accagccgac ttttccaggc cgcgatcaac gtcacggtcg aaaaccagcg gggagttcta
     5881 gccaaggtgg cctccgagat cgcctccgcc gcctccaaca tcgacgcgat cagcatggac
     5941 gaggaccggg cactttttac aaccatgcat ctcgtggtcg aagttgcgaa ccggcaacac
     6001 ctcgcgcgcg tgttgcgcgc tctgcggcgt atccccgacg tcaagaagat ttccaggatg
     6061 cgagagtaac agcagggatt tcttagcttt ttgtccgatg tcggcctgcc ccagccggtt
     6121 ttggtgtacg ctgacaatga acgtcagaag tgaaccccgg ggagcggcat gatcagtgca
     6181 cgagcgtgcg tcagcgtcat cgatgacgac gcgctggtct tgaggtcact cgagcgcctg
     6241 ctgcaatcgc gcgggttcga ggtgcacgcc tattcctcgc cgcaggcgtt cctcgagcag
     6301 cgcgatgccg acagccccgg ctgcattgtg atggatcttt ccatgcccgg cctgagcggc
     6361 ctcgagcttc agcaagcgct tgcgcacgcg gcggaccgcc ggccggtcgt gttcatcagc
     6421 ggccgcagca gcgtcgaggc gagcgtgcgg gcgatgaagg ccggcgcggt cgatttcctc
     6481 accaagccgt tcgacgaagc cgacctcgtc gcggcagtgt acgccgcggt ggaaaaggac
     6541 cgcgcctcgc gggagggcct cgccgagcgc gcctcgatca atctgcgcct ctccacgctg
     6601 acgccgcgcg agcgcgacgt cctgagccgc gtcgtggcgg gcaaactgaa caagcagatc
     6661 gcggccgagc tcggcaccgc cgaaaaaacg gtgaaggtgc accgcgcgcg aatgatgcgc
     6721 aaaatgcagt tcgactcgct cgccgagctg gtgcgcgcat ggacagtcac tcagcctaca
     6781 ccgccagcgc ctccccagat ctgacggcat ggggtt
//
LOCUS       DASYJA010000567         4835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1266218, whole genome shotgun sequence.
ACCESSION   DASYJA010000567 DASYJA010000000
VERSION     DASYJA010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266218"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="VGO02_12435"
     CDS             complement(<1..1083)
                     /locus_tag="VGO02_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478532.1"
                     /translation="MAALIQPGARLAETRPAPQAVAAPRRLRVGLYADSPLQPRWVLE
                     AFGRLAGSDFAALAAVAVEAKTAPDEPAFLPTVLSAYRGLDRWKFGSDLSEPKDLSLL
                     GQPLGQGAAHLERRELDVVFALGAVDDTALDGIARCGVWRFRVDGESEVVSGEPLSAS
                     GLDVRLAAGAVPRLAYQSWSRTYPLSVARNRRELLEKTAEFPARALRELHRSGREWLE
                     QCAPVAEEKSQRANANLIPLGKRILQRGIQKALSIDQWSLAFRFGPLRDIAGDLKGFT
                     RLVPPKDRDWADPFALEKGGRWYVFFEEKPYATGKAHISVIEILRDGRCSPAQRVLER
                     DYHLSYPFLLEHGNSLYMVPETAQNRS"
     gene            complement(1071..2351)
                     /locus_tag="VGO02_12440"
     CDS             complement(1071..2351)
                     /locus_tag="VGO02_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEV7478533.1"
                     /translation="MPTVAVVGLGYVGLPVAVRFARHRTTIGYDLSKKRIENLRHMVD
                     PTGEVSTAELMAAKRLRATHQPAEIELADFIIVAVPTPINAARQPDLTPLVSASETVG
                     RYMKQGATVIYESTVYPGATEEVCVPILERHSGMRWRQDFHVGYSPERINPGDKEHTF
                     AKILKVVSGDDAETLEKVAQLYSSVVSAGVFRASSIKVAEAAKVIENTQRDINIAFVN
                     ELAIIFEKMGLDTVEVLKAAGTKWNFLGFRPGLVGGHCIGVDPYYLTHKAELLGHHPE
                     VILAGRRINDMMGQHIARKTVQQMIHAGRNIKGARVNVLGLTFKENVPDIRNSKVADI
                     LRELKEFGVETFVHDPLASADDALHEYGVKLYEWDQLPAADALILAVPHKRLLAIPQA
                     MLQQKIVRQGCVIDIKSVLDAEALRREGLRVWRL"
     gene            complement(2398..3459)
                     /locus_tag="VGO02_12445"
     CDS             complement(2398..3459)
                     /locus_tag="VGO02_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV7478534.1"
                     /translation="MSRYEEVQQHIKRKPRRWLVTGAAGFIGSHLVEHLLRHGQSVVG
                     LDNFSTGKRENLGHVKNALGAEAWGRMRFIEGDIRSLDACRRACEGVELVLHQAALGS
                     VPRSIDDPLSSNDSNINGFLNMLTAARDARVARLVYASSSAIYGDHPALPKVEPVIGK
                     PLSPYGLTKGVNEQYAGVFAECYGFRSIGLRYFNVFGPRQNPDGEYSAVISAWIGALL
                     RGQTAYVNGDGKNGRDFCYVENVVQANLLAAMAEDPAATNQAYNVALGEQTTLNELFE
                     MIRLLLVPRLAHLKTVRPVHREPRRGDVRFSQADISKARKLLGFRPTHRVLEGLEKTV
                     EWYAANLPAAEPQKEVAHA"
     gene            complement(3425..4576)
                     /locus_tag="VGO02_12450"
     CDS             complement(3425..4576)
                     /locus_tag="VGO02_12450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV7478535.1"
                     /translation="MKLLFVTGSLVHGGAERHSITLANRLAERGHEVRFAYVKADHSQ
                     LERLRGVASVDCLNARSYLDFRSVSRLGILLKEAKPGAVLAANPYAMLYASAALRASG
                     AGAPLAVTYHTTVLQGAKSWLQMLYYRPFFWSADRLLFVCEAQRRYWRRRGVFGRSTQ
                     VIYNGIDTEHWVAGGTDRPAMRRALGYGEEDFVVAMSAVLRAEKNHVQMVDAIAMLRR
                     RGLPARALFIGDGPTRAAVEARARAAGVADSVLVTGFLQDVRPLLAACDAAALCSTAV
                     ETFSLAALEAMALEKPVVHSAVGGAAEMIRPWREGLLFPAGDTRALADCLAALTEAPL
                     RARMGAQARRTVEELFAERAMVERYEAALTELASTRSQREQIRRSAAAH"
     gene            complement(4573..>4835)
                     /locus_tag="VGO02_12455"
     CDS             complement(4573..>4835)
                     /locus_tag="VGO02_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478536.1"
                     /translation="AAGSVLAVYADRLLMLRCISRRTGISIGRMQDWRSLAWALASAV
                     LAGGLAWLLAEHAMQASGAFVRAVFGGSVLLLVYALLNLKRR"
BASE COUNT          710 a         1668 c         1690 g          767 t
ORIGIN      
        1 gctgcggttc tgcgcggtct cggggaccat atacagcgag ttgccatgct cgagcagaaa
       61 cggatacgac aggtggtagt cgcgctcgag gacgcgctgc gccggcgagc agcggccgtc
      121 gcgcaggatc tcgatcaccg aaatatgcgc cttgccggtg gcgtacggct tctcctcgaa
      181 gaacacgtac cagcgcccgc ccttctcgag cgcgaacgga tcggcccagt cgcggtcctt
      241 cggcggcacc aggcgcgtga agcccttcag gtcgccggcg atgtcgcgca gcgggccaaa
      301 cctgaaagcg agggaccact ggtcgatgct cagcgccttc tggattccgc gttgcaggat
      361 tctttttccg agcgggatca gattcgcgtt tgcacgttgc gatttttctt ccgcaaccgg
      421 tgcgcattgt tccagccatt cccttcccga gcggtgcagc tcgcgcaggg cgcgcgccgg
      481 gaactcggcg gtcttctcga gcagctcgcg gcggttgcgc gccaccgaca gcgggtaggt
      541 gcgcgaccag gactggtagg cgaggcgcgg caccgcgccc gcggcgaggc gcacgtccag
      601 gcccgaggcg ctgagcggct cgccggacac cacttcgctt tctccatcca cgcgaaagcg
      661 ccacacgccg cagcgcgcga tgccgtcgag cgccgtgtcg tccacggcgc cgagcgcgaa
      721 caccacgtcg agctcgcgcc gctcgaggtg cgcggcgccc tgcccgagcg gctggccgag
      781 gagcgacagg tccttcggct ccgaaaggtc gctgccgaac ttccagcgat cgaggccgcg
      841 gtaggccgag agcaccgtcg ggagaaatgc gggctcgtcc ggcgccgtct ttgcctcgac
      901 cgccaccgcg gcgagcgcgg cgaaatcgct gccggcgagc cgcccgaagg cctccagcac
      961 ccagcgcggc tgcagcggcg agtcggcgta caggccgacg cgcaggcggc gcggcgccgc
     1021 cacggcctgc ggcgcgggcc gcgtttcagc cagcctggcg ccgggctgta ttagagccgc
     1081 catacgcgca ggccttcgcg gcgcagcgcc tcggcgtcga gcaccgattt gatgtcgatg
     1141 acgcagccct ggcgcacgat cttctgctgc agcatggcct gcgggatggc gagcagccgc
     1201 ttgtgcggca cggcgaggat cagcgcgtcg gccgccggca gctggtccca ctcgtacagc
     1261 ttgacgccgt actcgtgcag cgcgtcgtcg gcgctcgcca gcgggtcgtg cacgaaggtc
     1321 tcgacgccga attccttcag ctcgcgcagg atgtcggcca ccttcgagtt gcggatgtcg
     1381 ggcacgttct ccttgaaggt cagcccgagc acgttgacgc gcgcgccctt gatgttgcgc
     1441 ccggcgtgga tcatctgctg cacggtcttg cgcgcgatgt gctggcccat catgtcgttg
     1501 atgcgccgcc cggcgaggat cacctccggg tggtggccca gcaactccgc cttatgcgta
     1561 aggtagtacg ggtcgacgcc gatgcagtgg ccgccgacca gccccgggcg gaagccgagg
     1621 aagttccact tggtgccggc cgccttcagc acctcgaccg tgtcgaggcc catcttctcg
     1681 aagatgatgg cgagctcgtt gacgaaggcg atgttgatgt cgcgctgcgt gttctcgatc
     1741 accttggccg cctcggccac cttgatcgac gaggcgcgga acacgccggc gctcaccacc
     1801 gagctgtaga gctgcgccac cttttcgagc gtctcggcgt cgtcgccgga gacgactttc
     1861 agtattttcg cgaaagtgtg ctccttgtcg cctggattga tccgctccgg cgagtagccg
     1921 acgtggaagt cctggcgcca gcgcatgccc gagtgccttt cgaggatcgg cacgcacact
     1981 tcctcggtcg ccccggggta gacggtcgac tcgtagatca ccgtcgcgcc ctgcttcatg
     2041 tacctgccga cggtttcgct cgccgaaacc agaggcgtga gatcgggctg gcgcgcggcg
     2101 ttgatcggcg tgggcacggc gacgatgatg aaatcggcca gctcgatctc cgccggctgg
     2161 tgcgtggcgc gcagccgctt ggccgccatc agctcggcgg tcgacacctc gccggtcgga
     2221 tcgaccatgt ggcgcaggtt ctcgatgcgc tttttcgaca ggtcgtagcc gatggtcgtg
     2281 cggtggcgcg cgaagcgcac cgccacgggc aggccgacgt agcccaggcc gaccacggcg
     2341 acggtgggca ggaccgcgct tctttcttcc tgggaatcta tctcttcgag aacttcctca
     2401 cgcatgggcc acttccttct gcggctcggc ggcggggagg ttcgccgcgt accactcgac
     2461 ggtcttctcc aggccctcga gcacgcggtg cgtcgggcgg aagccgagca gcttcctcgc
     2521 cttggagatg tcggcctgcg agaagcgcac gtcgccgcgg cgcggctcgc ggtgcaccgg
     2581 ccgcaccgtc ttcaggtgcg ccaggcgcgg caccagcagc aggcggatca tctcgaacag
     2641 ctcgttcagc gtggtctgct cgcccagcgc gacgttgtag gcctggttgg tcgccgccgg
     2701 gtcctcggcc atcgccgcca gcaggttggc ctgcaccacg ttctcgacgt agcagaagtc
     2761 gcggccgttc ttgccgtcgc cgttgacgta ggcggtctgg ccgcgcagca gcgcgccgat
     2821 ccaggccgag atcaccgcgg agtactcgcc gtcggggttc tggcgcgggc cgaagacgtt
     2881 gaaatagcgc agcccgatgc tgcggaagcc gtagcactcg gcgaacacgc cggcgtactg
     2941 ctcgttcacg cccttggtca ggccgtaggg cgagagcggc ttgccgatca ccggctcgac
     3001 cttgggcagc gccgggtggt cgccgtagat cgcgctcgag ctggcgtaga ccaagcgcgc
     3061 cactctggcg tcgcgcgccg ctgtcagcat gttgaggaag ccgttgatgt tgctgtcgtt
     3121 ggacgagagc gggtcgtcga tcgagcgcgg cacgctgccc agggccgcct gatgaagcac
     3181 cagctcgacg ccctcgcagg cgcggcggca ggcgtcgagc gagcggatgt cgccctcgat
     3241 gaagcgcatc ctcccccacg cttcggctcc cagcgcattt ttcacgtggc cgaggttctc
     3301 gcgcttgccg gtggagaagt tgtccaggcc gaccacgctc tgcccgtggc gcagcaggtg
     3361 ctcgaccagg tgcgagccga tgaagccggc ggcgccggtc accagccagc ggcgcggctt
     3421 gcgcttaatg tgctgctgca cttcttcgta tctgctcacg ttgactcctt gtggatgcca
     3481 gttctgtcag ggcggcctcg tagcgctcga ccatggcgcg ctcggcgaac agctcctcca
     3541 ccgtgcggcg cgcctgcgcg cccatgcgcg cgcgcagcgg cgcctcggtg agggccgcca
     3601 ggcagtcggc gagcgcgcgc gtgtcgccgg ccgggaaaag cagcccctcg cgccaggggc
     3661 ggatcatctc ggcggcgccg ccgacggccg agtgcaccac cggcttctcc agcgccatcg
     3721 cctcgagcgc ggcgagcgag aaagtctcga ccgcggtgct gcacagcgcc gccgcgtcgc
     3781 aggcggcgag cagcggccgc acgtcctgca ggaagccggt gaccagcacg ctgtcggcga
     3841 cgccggcggc gcgcgcgcgc gcctcgaccg cggcgcgcgt cggaccgtcg ccgatgaaca
     3901 gcgcgcgcgc cggcaggccg cgccggcgca gcatggcgat ggcgtcgacc atctgcacgt
     3961 ggttcttctc cgcgcgcagc accgccgaca tggcgacgac gaagtcctcc tcgccgtagc
     4021 cgagcgcgcg gcgcatcgcc ggccggtcgg tgccgccggc gacccagtgc tcggtgtcga
     4081 tgccgttgta gatcacctgc gtgctgcggc cgaagacgcc gcgccgccgc cagtagcggc
     4141 gctgcgcctc gcacacgaac agcagccggt cggcgctcca gaagaacggc cggtaataga
     4201 gcatctgcag ccacgacttg gcgccctgca ggaccgtggt gtggtaggtg accgcgagcg
     4261 gcgcccccgc gcccgaggcg cgcagcgcag ccgacgcata gagcatggcg tagggattcg
     4321 cggcgagcac cgcgcccggt ttggcttcct tgaggagaat gcccagccgg gaaaccgagc
     4381 ggaaatcgag gtagctcctg gcgttcaggc agtcgacgct cgccaccccg cgcagccgct
     4441 cgagctggct gtggtcggcc ttgacgtagg cgaagcggac ctcgtggccg cgctcggcga
     4501 ggcggttggc gagcgtgatg ctatggcgtt ccgcaccgcc gtgcaccaga gatcccgtga
     4561 caaaaagcag tttcatctgc gtttcaggtt cagcagcgcg tagaccagca ggagaacgct
     4621 gccgccgaag accgcgcgca cgaatgcgcc gctcgcttgc atggcgtgct cggccagcag
     4681 ccaggcgagc ccgccggcga ggaccgccga ggcgagcgcc caggcgagcg agcgccagtc
     4741 ctgcatgcgc ccgatcgaga tcccggtgcg gcgcgagatg caacgcagca tcagcaggcg
     4801 gtcggcgtag acggcgagca cgctgccggc ggccg
//
LOCUS       DASYJA010000568         3226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2991323, whole genome shotgun sequence.
ACCESSION   DASYJA010000568 DASYJA010000000
VERSION     DASYJA010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2991323"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VGO02_12460"
     CDS             <1..462
                     /locus_tag="VGO02_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015421982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478537.1"
                     /translation="LDGQIGCMVNGAGLAMATMDTIKLFGGSPANFLDVGGGATAEKV
                     TAAFKIMLSNPKVKAILVNIFGGIMKCDTIAAGVVAAARETKLSVPLVVRMKGTNEDL
                     GKKILKDSGLPIISADNMGEAGQKVVASIAKKKAKPVKKKKAKAAKKRKKK"
     gene            459..1343
                     /gene="sucD"
                     /locus_tag="VGO02_12465"
     CDS             459..1343
                     /gene="sucD"
                     /locus_tag="VGO02_12465"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEV7478538.1"
                     /translation="MSILIDKNTRVVTQGITGKTGMFHTQTSRAYAEGKTCYVAGVNP
                     KKGGESFEGIPIFATVKEAKEKAGANTSVIYVPPPFAAAAIWEAVEADLDLVICITEG
                     IPVRDMIEVRERMRKQKRRALLVGPNCPGVITPGVIKIGIMPGHIHKKGRVGVVSRSG
                     TLTYEAVGQLTELGIGQSSAVGIGGDPVNGMKHIDVMKLFNEDPQTDAVIMIGEIGGS
                     DEEDAARWVKANMKKPVVGFIAGVTAPPGKRMGHAGAIISGGKGTAQEKLAVMESCGI
                     KVTKNPAEMGRLLKSVLK"
     gene            1484..>3226
                     /locus_tag="VGO02_12470"
     CDS             1484..>3226
                     /locus_tag="VGO02_12470"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEV7478539.1"
                     /translation="MVFFQTILLAGYGYADLTMRLGARRQAMLHVALLAASLLFLPIL
                     ASSGWKPQGEEEPVGRILLLLLATIGLPYFLLSSTTPLLQAWYYRRWHVAVPYRLFAL
                     SNFASLLALLGFPLLFEPAFDLKALGWAWSFLYAGFVVLCGVVAFMSGNGSREKIHSA
                     TKESPAAATQLLWLALSAMGSVMLLAVTNHITQNIASVPFLWVLPLALYLVTFILAFD
                     HPRWYIRPAFVAALMVMLPLMAWFVPSLDLHIAAPLYLVGMFVACMVCHGELARLKPD
                     PAHLTRFYLMISVGGAAGAVLVAIVAPLALSGYFELGIALVVLAALLTFRMKKVFLWV
                     GVLMTAVTVFLVAKGAKDYTEGVRVMERDFYGVVRTVDFFHPVAYRSMYHGGIMHGGQ
                     LQGDSFRNTPADYFNPGSGYGRVFRSLREMRPKKPLQVGVIGLGAGVIGGWMRPGDSL
                     VFYEISPRVVDIAKREFTYLADTAARTELVMGDGRLSLEREPPRGYDVLGIDAFSGDS
                     IPMHLVTREAMAIYVRHIKPDGVIVFQATNRFIDLLPVVKRLASEFGLQAVNIGDAPE
                     GEEGPEYWYSSTDQV"
BASE COUNT          541 a         1054 c         1068 g          563 t
ORIGIN      
        1 ctcgacggcc agatcggctg catggtgaac ggcgccggcc tggccatggc gaccatggac
       61 acgatcaagc tgttcggcgg ctcgccggcc aacttcctcg acgtcggcgg cggcgccacc
      121 gccgagaagg tcaccgccgc cttcaagatc atgctctcca acccgaaggt gaaggcgatc
      181 ctggtcaaca tcttcggtgg catcatgaag tgcgacacca tcgccgccgg cgtggtggcc
      241 gcggcccgcg agaccaagct gtcggtgccg ctggtggtgc gcatgaaggg caccaacgag
      301 gacctcggca agaaaatcct gaaggactcc ggcctgccga tcatctcggc cgacaacatg
      361 ggcgaggcgg ggcagaaggt ggtcgcctcg atcgccaaga agaaagccaa gccggtgaaa
      421 aagaagaaag ccaaggcggc gaagaagagg aagaagaaat gagcatcctc atcgacaaga
      481 acacgcgcgt ggtgacgcag ggcatcaccg gcaagaccgg catgttccat acgcagacca
      541 gccgcgccta tgcggaaggg aaaacctgct atgtggctgg agtgaacccc aagaagggcg
      601 gagagagctt cgaggggatt ccgatctttg cgaccgtgaa ggaagcaaag gaaaaggccg
      661 gcgcgaatac ctcggtgatc tacgtgccgc cgcccttcgc cgcggcggcg atctgggagg
      721 cggtcgaggc cgacctcgac ctggtcatct gcattaccga gggtatcccg gtgcgcgaca
      781 tgatcgaggt gcgcgagcgc atgcgcaagc agaagcgccg tgcgctgctg gtcggcccga
      841 actgcccggg cgtgatcacg ccgggggtga tcaagatcgg catcatgccg ggccacatcc
      901 acaagaaggg gcgcgtcggc gtggtgtcgc gctcgggcac gctcacctat gaagcggtcg
      961 gccagctcac cgagctcggc atcggccagt cgagcgcggt gggaatcggc ggcgatccgg
     1021 tcaacgggat gaagcacatc gacgttatga agctgttcaa cgaggacccg caaaccgacg
     1081 ccgtcatcat gatcggcgag atcggcgggt ccgacgaaga ggatgcggcc cgttgggtga
     1141 aagcgaacat gaaaaagccg gtggtgggat tcatcgccgg ggtgaccgcg ccgccgggca
     1201 agcgcatggg gcacgcgggc gccatcatct ccggcggcaa gggcacggcg caggagaagc
     1261 tggcggtgat ggagtcctgc ggcatcaagg tgacgaagaa ccccgccgag atgggacggc
     1321 ttctaaaatc cgtactgaag taatccgcct cccgcttctg gccacgctgc cgtacgcggc
     1381 gacgattttc ctttcgtcgt tcctgctgtt cctcgtccag ccggtcatcg ccaagcagat
     1441 cctgccctgg ttcggcggct ccgccggcgt ctggaccacc tgcatggtgt tcttccagac
     1501 catcctgctg gcgggctacg gttacgccga cctgaccatg cgcctgggcg cgcgccgcca
     1561 ggcgatgctg cacgtcgcgc tgctcgccgc ctcgctgctg ttcctgccga tcctcgcctc
     1621 gagcggctgg aagccgcagg gcgaggagga gccggtcggc cgcatcctgc tcctgctgct
     1681 ggccaccatc ggcctgccgt acttcctgct ctcgagcacc acgcccctgc tgcaggcgtg
     1741 gtactaccgg cgctggcacg tcgccgtgcc ttaccgcctg ttcgccctct ccaatttcgc
     1801 atcgctgctc gcgctgctcg gcttcccgct gctgttcgag ccggccttcg acctgaaggc
     1861 gctcggctgg gcctggtcgt tcctgtacgc cggcttcgtg gtgctgtgcg gagtcgtggc
     1921 gttcatgtcc gggaatggtt ctcgggaaaa aattcattcg gcaacaaagg aaagccccgc
     1981 agccgctact cagctgctgt ggctcgccct gtcggcgatg ggctcggtga tgctgctcgc
     2041 cgtgaccaac cacatcacgc agaacatcgc gagcgtgccg ttcctgtggg tgctgccgct
     2101 cgcgctgtac ctcgtcacct tcatcctcgc cttcgaccat ccgcgctggt acatccggcc
     2161 ggcgttcgtc gccgcgctga tggtgatgct gccgctgatg gcctggttcg tgccctcgct
     2221 cgacctgcac atcgcggcgc cgctctacct cgtcggcatg ttcgtcgcct gcatggtgtg
     2281 ccacggcgag ctcgcgcgcc tcaagcccga cccggcgcac ctcacccgct tctacctgat
     2341 gatctcggtc ggcggcgccg ccggcgcagt gctggtggcg atcgtcgcgc cgcttgccct
     2401 gtcgggctac ttcgagctgg gcatcgcgct ggtggttctc gcggcgcttt tgactttcag
     2461 aatgaaaaaa gtatttctct gggtcggggt gttgatgacc gcggtcaccg tgttcctggt
     2521 cgcaaagggc gccaaggact acaccgaggg cgtgcgcgtc atggagcgcg acttctacgg
     2581 cgtggtgcgc acggtcgact tcttccatcc ggtcgcctac cgctccatgt accacggcgg
     2641 catcatgcac ggcggccagc tgcagggcga ctcgtttcgc aacacgccgg ccgactactt
     2701 caacccgggc tcgggctacg ggcgcgtgtt ccgctccctg cgcgaaatgc gcccgaagaa
     2761 gccgctgcag gtgggcgtga tcggcctggg cgcgggcgtg atcggcggct ggatgcggcc
     2821 gggcgactcg ctggtcttct acgagatcag cccgcgcgtg gtcgacatcg ccaagcgcga
     2881 gttcacttac cttgcggaca ccgcagcgcg caccgagctg gtgatgggcg acggccggct
     2941 atcgctcgag cgcgagccgc cgcgcggcta cgacgtgctg ggtatcgacg ccttctccgg
     3001 cgactcgatc ccgatgcacc tggtgacgcg cgaggcgatg gcgatctacg tgcgccacat
     3061 caagcccgac ggcgtgatcg tgttccaggc caccaaccgc ttcatcgatc tgctgccggt
     3121 ggtgaagcgc ctcgcctccg agttcggcct ccaggcagtg aacatcggcg acgcgcccga
     3181 gggcgaagag ggcccggagt actggtattc gtccaccgac caggtg
//
LOCUS       DASYJA010000569         2717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2083649, whole genome shotgun sequence.
ACCESSION   DASYJA010000569 DASYJA010000000
VERSION     DASYJA010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2083649"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VGO02_12475"
     CDS             <1..399
                     /locus_tag="VGO02_12475"
                     /inference="COORDINATES: protein motif:HMM:NF039902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478540.1"
                     /translation="ARTLHLLPYAAVRGRLPANVGEALWLAVRGNLTTLADAATWAAI
                     VNGSLAPLLEDPPFLEEAAARLPAEPWSEATWKEWTGAVSAATGRKGRVLFHPLRLAL
                     TARETGPEMAKLLPLIGRAKADQRLRGQTA"
     gene            complement(476..629)
                     /locus_tag="VGO02_12480"
                     /pseudo
     CDS             complement(476..629)
                     /locus_tag="VGO02_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249274.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
     gene            782..2113
                     /gene="cysS"
                     /locus_tag="VGO02_12485"
     CDS             782..2113
                     /gene="cysS"
                     /locus_tag="VGO02_12485"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV7478541.1"
                     /translation="MSLVIYNTLSREKEPFAPLDAGHVRLYVCGPTVYDYVHIGNARP
                     VVAFDVLYRLLKRLFPRVTYVRNITDIDDRIMVRAADNGETIEQLTTRTGNAYQSDMR
                     RLGALAPDVEPRATQYVAEMVAMIQRLVDNGHAYAAEGHVLFSVPSMSDYGRLSRRTR
                     DELVAGARVEVAPYKKDPADFVLWKPSANNQPGWPSPWGRGRPGWHIECSAMSAKHLG
                     ETFDIHAGGLDLIFPHHENEIAQSRSAFGHAIMAKYWMHNGFLNISGEKMSKSLGNFF
                     TVHELLDQFPGEVIRLLLLSAHYRQPLDFTHEGLTQAKATLDRWYGVLRGKDARPANA
                     VPQSVEDALSDDLNTPLAISAIHQLEDPAELRAGAQALGLLQQDAEAWFRWTPAGSSG
                     PSDPEIEAAIAARQAARKARDFKESDRIRDELKAKGVLLEDGAKGTTWKRA"
     gene            complement(2161..2427)
                     /locus_tag="VGO02_12490"
     CDS             complement(2161..2427)
                     /locus_tag="VGO02_12490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEV7478542.1"
                     /translation="MLVVLAEARFDARDTGQVRAVARPMIEASRAEPGCAGYDYAFDL
                     LEPDLMRVRELWRDEQALKDHFATPHMAAFLKGLRDLRPKTVTV"
     gene            complement(2439..>2717)
                     /locus_tag="VGO02_12495"
     CDS             complement(2439..>2717)
                     /locus_tag="VGO02_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7478543.1"
                     /translation="GLSIARQASVPALVKCPRAFSETDFRADMRAFTLPTLIVYGTAD
                     MPSTAKNAARTAAAIAGSRVERYEGAPHGLFLTDPERFNRDLLRFVRS"
BASE COUNT          467 a          929 c          904 g          417 t
ORIGIN      
        1 gcgcgcacgc tccacctgct gccctacgcg gcggtgcgcg gccgcctgcc ggccaatgtc
       61 ggcgaagctc tgtggctcgc cgtgcgcggc aacctgacga cgctggcgga cgctgcgacc
      121 tgggccgcca tcgtcaacgg ctcgctggcg ccgctgctcg aggatccgcc cttcctcgag
      181 gaggcggcgg cgcgtttgcc ggccgagccg tggagcgagg ccacctggaa ggaatggacg
      241 ggagcggtgt cggcagcgac cggacgcaag ggccgcgtac tgttccatcc gctgcggctc
      301 gcgctgacgg cgcgagagac cgggcccgag atggcgaaac tcctgccgct gatcggccgc
      361 gcgaaggccg accagcgcct cagggggcag acggcatgaa gaccggcgcg ttgaccagca
      421 gcatgctggc cgtggtaccc agggtgacga gcaccggcga attgaagcgt gcggtgagcg
      481 cgtggtccgt cagagaaggc caacaggatt cccagggtga tcgggaccgg cttgcgctag
      541 cgcgccgcca gcatcagcgc gagcagctgg gtcttgtcgc cgatctcggc ggcagcgatc
      601 acggcggcag agacaaaaaa cgcttccatt ggcgcgcatt tgcacagaaa gaacgcatcg
      661 gataaactac ctgatgtcgc tctttctatt ccccggcgct gtttctgcgc gatgtaactg
      721 aagtcgcccc cgctttgcgg gcggccggac ttcgtacact gctgatcggg gagtaggggc
      781 catgtccctc gtcatctaca acacgctgtc gcgcgaaaaa gaaccatttg caccactcga
      841 cgcagggcat gtgcgtctgt atgtctgcgg cccgaccgtc tacgactacg tgcatatcgg
      901 caatgcgcgg cccgtcgtgg ccttcgacgt gctctatcgc ctgctgaagc ggctttttcc
      961 gcgcgtcacc tacgtgcgca acatcaccga tatcgacgac cgcatcatgg tgcgtgccgc
     1021 cgacaacggc gagaccatcg agcagctgac cacgcgcacc gggaacgcct atcaaagcga
     1081 catgcgccgg ctgggcgcgc ttgcgccgga cgtcgagcct cgcgccaccc agtacgtcgc
     1141 cgagatggtc gccatgatcc agcgcctggt cgacaacggc catgcctatg ccgccgaggg
     1201 ccatgtcctg ttcagcgtgc cgagcatgag cgattacggc cgcctgtcgc ggcgcacgcg
     1261 cgacgagctg gtggccggcg cgcgggtcga ggtcgcaccc tacaagaagg accccgccga
     1321 cttcgtgctg tggaagccgt cggccaacaa ccagccgggc tggccgagcc cgtggggccg
     1381 cggccggccg ggctggcaca tcgaatgctc ggcgatgagc gccaagcatc tcggcgagac
     1441 cttcgacatc catgccggcg gcctcgacct catcttcccg caccacgaga acgagatcgc
     1501 gcagagccgc agcgccttcg gccacgccat catggcgaag tactggatgc acaatgggtt
     1561 cctgaacatc tcgggcgaga agatgagcaa gtcgctcggc aacttcttca ccgtgcacga
     1621 gctgctggac cagttcccgg gtgaggtgat ccggctgctg ctgctctcgg cgcactaccg
     1681 ccagccactc gacttcacgc acgagggact gacccaggcc aaggccacgc tcgatcgctg
     1741 gtacggcgtg ttgcgtggaa aggatgctcg gcccgcgaat gcggtgccgc aatcggtcga
     1801 ggacgcgctg tcggacgacc tcaatacgcc gctcgccatc agcgccatcc atcagctcga
     1861 agatccggca gaattgcgcg caggcgccca ggcactcggc ctgctgcagc aggacgccga
     1921 agcctggttc cgctggacgc cggccggctc gtccggtccg agcgatcctg agatcgaggc
     1981 ggcgattgcc gcccgtcagg ccgcgcgcaa ggcccgcgac ttcaaggagt cagaccgcat
     2041 ccgcgacgag ctcaaggcca agggtgtcct cctcgaggat ggcgccaagg gcacgacctg
     2101 gaaacgcgcc taggctgagg gcgcgcccgg catcttcttt tcgggcccca gctcgtagca
     2161 ctacaccgtg acggtcttgg gccggaggtc gcgcagcccc ttcaggaacg ccgccatgtg
     2221 cggcgtggcg aagtgatcct tcagcgcctg ctcatcccgc cacagctcgc gcacgcgcat
     2281 caggtcgggc tcgagcaggt cgaaggcata gtcgtagccg gcgcagcccg gctcggcgcg
     2341 cgacgcctcg atcatcggcc gcgcgacggc gcgcacctgc ccggtgtccc tggcgtcgaa
     2401 ccgggcctcg gcaagcacga ccagcatggc aatctccttc agctccgtac gaacctcagc
     2461 aagtcgcggt tgaaacgctc ggggtcggtc aggaacaacc cgtgcggggc gccttcgtaa
     2521 cgctcgaccc ggctgccggc aatcgccgct gccgtgcgcg ccgcgttctt cgctgtcgag
     2581 ggcatgtcag ccgtgccata gacgatcaag gtcggcaggg tgaaggcgcg catgtcggcg
     2641 cgaaagtcgg tctcgctgaa ggcgcgcggg cacttgacca gcgccggcac cgaggcctgc
     2701 cgcgcgatcg acaggcc
//
LOCUS       DASYJA010000570         5301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_995663, whole genome shotgun sequence.
ACCESSION   DASYJA010000570 DASYJA010000000
VERSION     DASYJA010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995663"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VGO02_12500"
     CDS             <1..410
                     /locus_tag="VGO02_12500"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HEV7478544.1"
                     /translation="TKPDLPFEVRAFALIPPDRAVLHRKIEERFALMLQAGLVDELRG
                     LRRRFDLKASMPSMRAVGYRQVWEMLEDNAGAKTMRERAVAATRQLAKRQMTWLRSLP
                     GLVRLDAGGAQGGAVAVELLLDQIGEPLGRAGR"
     gene            complement(312..1262)
                     /locus_tag="VGO02_12505"
     CDS             complement(312..1262)
                     /locus_tag="VGO02_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7478545.1"
                     /translation="MGRLVCGLVLAAVCWAAHAQFPAKPVRVLVPFPAGSATDTVGRV
                     LAAAVSQAIGQPVVVENKAGADGAIAAAEVAKAPADGYTLLLATNSPLSAVPAMKKVP
                     PYDPVADFTPITDVGRYTFFIVVHPDVPAKSLDEFIAHARANPGRLNYATGNTSGIVS
                     TAFFAAQAQIKLVHVPYKGEPQALTDLVAGRVQVMFCSSGTSLPMIREGRLRALVTTL
                     PRRSASLPEVPTIAEAGMPQFSITSWAGLFGPANMPRPVVERLNREFVAAMARADVQA
                     ALEKQGFALSPSSPERLADLVKEQLDSYRATLRAAGVEPD"
     gene            complement(1273..1500)
                     /locus_tag="VGO02_12510"
     CDS             complement(1273..1500)
                     /locus_tag="VGO02_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478546.1"
                     /translation="MDYLDLLQWPAMAVTVAAAWLVGSRLPQRRAAGFWCFLASNLLW
                     VVWGWHDGAYALIGLQFFLAALNIRGVWKNE"
     gene            1546..2457
                     /gene="gnd"
                     /locus_tag="VGO02_12515"
     CDS             1546..2457
                     /gene="gnd"
                     /locus_tag="VGO02_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEV7478547.1"
                     /translation="MEIGIVGLGRMGAGMARRLARGGVNVVCYDQSDAARAALAAESG
                     VVCAENLAALCARLHGERVIMLMLPAGSPVEDTIRDLLPLVSAGDTLVDGGNSYYRDS
                     KRRALGLSQQGLRYIDAGVSGGVHGLASGFCLMLGGTAKSIEMFEPFARILAPDVDRG
                     WLHCGPSGAGHYAKMIHNGIEYGMMQSLAEGFALLAARPELDIDVGRLAETWRHGSVV
                     RSWLLDLCAEILKDNENLADIAPVVADSGEGRWTALESIELGVPAPVITLALMGRFSS
                     QGGSDYARKLLAVMRSRFGGHAVQKSG"
     gene            2454..2927
                     /locus_tag="VGO02_12520"
     CDS             2454..2927
                     /locus_tag="VGO02_12520"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEV7478548.1"
                     /translation="MIVIVMGVSGVGKTTIGQALALELGWRYLDADDYHPAANVAKMA
                     AGTPLDDADRWPWLDKLNALLQQEKNAVLGCSALKEAYRQRLTAWLKEFRIVYLRGSF
                     ELLSQRAGERKHRFMPASLLKSQFEALEPPRGAIEVDVAQSVEACVAAIRLQLGR"
     gene            complement(2933..4450)
                     /gene="ggt"
                     /locus_tag="VGO02_12525"
     CDS             complement(2933..4450)
                     /gene="ggt"
                     /locus_tag="VGO02_12525"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEV7478549.1"
                     /translation="MAAAHPLAVQAGYDVLKRGGSALDAAIAVQMVLGLAEPSASGIG
                     GGAFMLHWSARDRTLRTYDGRETAPAAARPDLFLEEKKRPLEFLDAVASGRSVGVPGV
                     LRMLELAHQRHGKLPWAELFEPAISLAQNGFAASPRLRTLLEREVVLKQDPAARKIYY
                     EPGGRIVNPEYGKTLRAIAEGGAEAFYRGPIAQDIVRAVRSHAKPGGMTEADLSQYRA
                     LERDALCGNYRVWVVCSMPPPSAGGVALLQILGVLERKGFSRAAPQSARALHLLAEAE
                     RLAYADRARHMGDADFVPVPIKPLLAGKYLDERAALIGERALTVAPPGSFESGTSHLV
                     VADAEGNVLSMTTTIESIFGSRIMVRGFLLNNQLTDFDFVPGGPNQVQGGKRPRSSTT
                     PVIAFDADGAVRLAAGSAGGSMIINYVAKTLVAVLDWDLDIQAAVAAPNLGNRSGPTE
                     IEQGSAYEALAPALRARGHEVSIRELPSGSHGIERVPGGWRGGADPRRDGTAKGD"
     gene            complement(4543..>5301)
                     /locus_tag="VGO02_12530"
     CDS             complement(4543..>5301)
                     /locus_tag="VGO02_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEV7478550.1"
                     /translation="GPITDNAGGIAEMADLPDSVRAITDPLDAVGNTTKAVTKGYAIG
                     SAGLAALVLFADYTHALEAAGKSITFDLSNHMVIIGLLIGGLVPYLFGAMAMEAVGRA
                     AGSVVVEVRRQFKEIKGIMEGTAKPQYGVAVDLLTKAAIKEMMIPSLLPVLVPILVGL
                     ILGPQALGGVLMGTIVTGLFVALSMSTGGGAWDNAKKYIEDGHHGGKGSDAHKAAVTG
                     DTVGDPYKDTAGPAVNPLIKIINIVALLIVPLMV"
BASE COUNT          839 a         1825 c         1818 g          819 t
ORIGIN      
        1 gcacaaaacc ggatttgccc ttcgaagtcc gtgccttcgc gctcatccca ccggatcgcg
       61 cggtgctgca taggaagatc gaggaacgtt tcgctctcat gctgcaggcc ggcctggtgg
      121 atgagttgcg aggcctgcgc agacgattcg atctgaaggc aagcatgcca agcatgcgcg
      181 cggtgggcta ccggcaggtc tgggagatgc tggaagacaa cgccggcgcg aaaaccatgc
      241 gcgagcgcgc cgttgctgcc acgcgccagc tcgccaagcg gcagatgacc tggctgcgca
      301 gcctgccggg cctagtccgg ctcgacgccg gcggcgcgca gggtggcgcg gtagctgtcg
      361 agctgctcct tgaccagatc ggcgagccgc tcgggcgagc tgggcgatag agcgaagccc
      421 tgtttctcca gcgccgcctg gacatcggcg cgggccatcg cggcgacgaa ctcccggttc
      481 aggcgttcga ccaccggccg gggcatgttc gccggaccga aaagccctgc ccacgacgtg
      541 atcgagaact ggggcatgcc cgcttcggca atggtcggca cctccggcag cgacgcgctg
      601 cgccgcggca gcgtcgtcac cagcgcgcgc agcctgcctt cgcggatcat cggcagcgac
      661 gtgccggacg agcagaacat cacctgcacc cggccggcca cgaggtcggt gagcgcctgc
      721 ggttcgccct tgtagggaac gtgcaccagc ttgatctgcg cctgcgcggc gaagaaggcg
      781 gtggagacga tgccgctggt gttgccggtg gcgtagttga gcctgcccgg gttggcgcgc
      841 gcgtgcgcga tgaattcgtc gagcgatttc gccggcacgt ccgggtgcac gacgatgaag
      901 aaggtgtagc ggccgacgtc ggtgatcggc gtgaaatcgg ccaccgggtc gtacggcggc
      961 actttcttca tcgccgggac cgcggacagc gggctgttgg tggcaagcag gagcgtgtag
     1021 ccgtcggccg gcgccttcgc cacctcggcg gcggctatgg cgccgtccgc gcccgccttg
     1081 ttctccacca ccactggctg gccgatggcc tgcgacacgg cggcggcgag gacccggccg
     1141 acagtatcgg tggccgagcc ggccgggaac ggcaccagca cgcgaaccgg cttcgccggg
     1201 aactgcgcat gagccgccca gcacacggcc gcgagcacca gtccgcacac cagcctgccc
     1261 atgaaggcct cctcattcgt tcttccagac gccccggata ttaagcgccg cgaggaaaaa
     1321 ctgcaggccg atgagcgcat aggcgccgtc gtgccagccc cacacgaccc acagcaggtt
     1381 gctggcgagg aagcaccaga agccggcggc gcgccgctgc ggcaggcgcg agccgaccag
     1441 ccaggccgcc gccacggtga cagccatcgc cggccactgc aggagatcga gatagtccac
     1501 agctacgcta gaccctagaa tgcggcctct cgttcgggag ctttcatgga aatcggcatc
     1561 gtcggcctgg gccggatggg cgcgggcatg gcgcggcgcc tggcgcgcgg cggcgtgaac
     1621 gtggtctgct acgaccagtc ggacgccgcg cgcgccgcgc ttgccgcgga gtccggcgtg
     1681 gtctgcgccg agaacctcgc cgcgctctgc gcgcgcctgc acggcgagcg cgtgatcatg
     1741 ctgatgctgc cggccggcag cccggtcgag gacacgatcc gcgacctcct gccgctcgtc
     1801 tcggcgggcg acaccctggt cgacggcggc aacagctact accgcgattc caagcgccgc
     1861 gcgctggggc tgtcgcaaca ggggctgcgc tacatcgacg ccggcgtctc cggcggcgtg
     1921 cacggcctgg ccagcggctt ttgcctgatg ctcggcggca cggcgaagtc gatcgagatg
     1981 ttcgagccgt tcgccaggat cctggcgccg gacgtcgacc gcggctggct gcattgcggg
     2041 ccgagcggcg ccggccacta cgcgaagatg atccacaacg gcatcgagta cgggatgatg
     2101 cagtcgctcg ccgagggctt cgcgctgctc gccgccagac ccgagctgga catcgacgtc
     2161 gggcggctcg ccgagacctg gcgccacggc agcgtagtgc gctcctggct gctcgatctg
     2221 tgcgccgaga tcctcaagga caacgaaaat ctcgccgaca tcgcgccggt ggtggcggac
     2281 tcgggcgaag ggcgctggac cgcgctcgag tcgatcgagc tcggcgtgcc ggcgccggtg
     2341 atcacgcttg cgctgatggg ccgcttctcc agccagggcg gctccgacta cgcgcgcaag
     2401 ctcctcgccg tgatgcgcag ccgcttcggc gggcacgccg tgcagaagag cgggtgatcg
     2461 tcatcgtgat gggcgtgtcc ggggtcggca agacgaccat cgggcaggcg ctggcgctcg
     2521 agcttggctg gcgctacctg gacgccgacg actaccaccc ggcggcgaac gtcgccaaga
     2581 tggcggccgg aacgcccctg gacgacgccg atcgctggcc ctggctcgac aagttgaacg
     2641 cgctcctgca acaggaaaaa aacgcagtgc tcggctgctc ggcgctgaag gaggcctacc
     2701 ggcagcgtct cactgcctgg ctgaaggagt ttcgcatcgt ctacctgcgc ggaagcttcg
     2761 aactgctgag ccagcgcgcc ggggagcgca agcaccgctt catgcccgcc tcgctgctca
     2821 agagccagtt cgaggcgctc gagccgccgc gcggcgcgat cgaggtcgac gtagcgcaat
     2881 cggtcgaggc ctgcgtcgcc gcgatccgct tgcagctcgg ccgctagctt tctcaatccc
     2941 ctttcgccgt gccgtcccgg cgcgggtcgg cgccgccgcg ccagccgccc ggcacgcgct
     3001 cgatgccgtg cgagccgctc ggcagctcgc ggatcgagac ctcgtggccg cgggcgcgca
     3061 gcgccggtgc cagggcttcg taggcgctgc cctgctcgat ttcggtcggc ccgctgcggt
     3121 tgccgaggtt gggcgcggcg acggcagcct ggatatcgag atcccagtcg agcaccgcga
     3181 ccagcgtctt ggcgacgtag ttgatgatca tggagccgcc ggccgagccg gcggcgaggc
     3241 gcaccgcgcc gtcggcgtcg aacgcgatca ctggcgtcgt gctgctgcgc ggccgcttgc
     3301 cgccttgcac ctggttcggt ccccccggga cgaaatcgaa gtcggtgagc tgattgttga
     3361 ggaggaagcc gcgcaccatg atacggctac cgaagatgct ctcgatggtg gtggtcattg
     3421 agagcacgtt gccctcggcg tcggcgacga ccaggtggct ggtgccggat tcgaagcttc
     3481 cgggtggcgc gacggtcagc gcccgctcgc cgatcagtgc ggccctttca tccaggtact
     3541 tgcctgccag caagggtttg atgggaaccg gtacgaagtc cgcatcgccc atgtgtctcg
     3601 cgcgatcggc gtaagcaagc cgctccgcct cggcgagcag gtgcagcgcc cgcgccgatt
     3661 gcggtgccgc ccgcgagaat ccctttcgct cgagcacgcc gaggatctgc agcagcgcga
     3721 cgccgcccgc gctcggcggc ggcatcgagc acaccaccca cacgcgatag ttcccgcaca
     3781 gcgcgtcgcg ctccagggcc cggtactgcg acaggtcggc ctcggtcatg ccgccgggct
     3841 tcgcgtgcga gcgcactgcg cgcacgatgt cctgcgcgat cggtccgcgg tagaaggcct
     3901 ccgcgccgcc ctcggcgatc gcgcgcaggg tcttgccgta ttccggattg acgatgcgtc
     3961 cgcccggttc gtaatagatc tttctcgctg cgggatcctg tttgaggacg acctcgcgct
     4021 caagcaacgt gcgcaggcgc ggcgaagccg caaacccgtt ctgcgcgagc gaaatcgccg
     4081 gctcgaacag ctccgcccat ggcagcttgc cgtggcgctg gtgcgcaagc tcgagcatgc
     4141 gcagcacgcc tggcacgccc accgagcggc cgctcgctac cgcgtccagg aattccaacg
     4201 gtcgtttttt ctcctccaag aacaaatccg gccgggcggc cgcgggcgcc gtctcgcgtc
     4261 cgtcgtaggt gcgcagcgtg cggtccctgg cggaccaatg gagcatgaag gcgccgccgc
     4321 cgatgcccga ggcggaaggc tccgccaggc cgagcaccat ttgcaccgcg atcgccgcgt
     4381 cgagcgccga gccaccgcgc ttgagaacgt cgtatcccgc ttgcaccgcg agcgggtgag
     4441 ccgccgccac gatgaagtgc ggcgcagtct gggcctggca gcccgccgca aaaacaaaaa
     4501 gggcgaccgc agccgccctt ttcagtaaaa aaacacgaac catcacacca tcaagggcac
     4561 gatcagcagc gccacgatgt tgatgatctt gatgagcggg ttcaccgccg ggccggccgt
     4621 gtccttgtaa ggatcgccga cggtgtcgcc ggtgaccgcc gccttgtggg cgtccgagcc
     4681 cttgccgccg tggtggccgt cctcgatgta cttcttggcg ttgtcccagg cgccgccgcc
     4741 ggtcgacatc gacagcgcga cgaacaagcc cgtcacgatg gtgcccatca gcacgccgcc
     4801 cagggcctgc ggtccgagaa tcaaccccac caggatcggt accagcaccg gcagcagcga
     4861 cgggatcatc atctccttga ttgccgcctt ggtgagcagg tcgaccgcga caccgtactg
     4921 cggcttcgcg gtgccttcca taatgccctt gatctccttg aactggcggc gcacttctac
     4981 caccaccgag cccgcggcgc ggccgaccgc ttccatcgcc atcgcgccga acaggtacgg
     5041 caccaggccg ccgatcagca ggccgatgat caccatatgg ttcgacagat cgaaggtgat
     5101 gctcttgccc gcggcttcga gcgcgtgcgt gtagtcggcg aacagaacga gcgcggcgag
     5161 tcccgccgag ccgatcgcat agcccttggt gacggccttg gtggtgttgc cgactgcgtc
     5221 aagcggatcg gtgattgcgc gcaccgagtc gggcagatcg gccatctcgg caatcccgcc
     5281 ggcgttatcg gtgatcggac c
//
LOCUS       DASYJA010000571         1505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3265451, whole genome shotgun sequence.
ACCESSION   DASYJA010000571 DASYJA010000000
VERSION     DASYJA010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3265451"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VGO02_12535"
     CDS             complement(<1..479)
                     /locus_tag="VGO02_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478551.1"
                     /translation="MHLLGRSALLGALVGLLSFFIGEAAYAQFGVVSEDVRIPVAAGN
                     YSIATRIIRPEGSGRFGAVVLNHGVAGTRAERMKEAPEQLLAAATVFAQHGYVVVMPL
                     RRGFGATGGDYAEDPGPCSNPDFRKAENAASQDVMAAYDYARTLPYVDGSRMILAGQS
                     "
     gene            complement(596..>1505)
                     /locus_tag="VGO02_12540"
     CDS             complement(596..>1505)
                     /locus_tag="VGO02_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M20"
                     /protein_id="HEV7478552.1"
                     /translation="SYDLPAYLEFLAPRIKTPDLVIGLDSGCGNYEKLWGTTSLRGLV
                     NGVLSVEVLTEGVHSGDASGVVASSFRILRELLERVDAADTGVVRHAAFHAPIPPERG
                     EQAKRAAQVLGEEIWRKFPFAGGMKPMAEDLSDLVLNRSWRPMLAVTGAEGLPLPANA
                     GNVLRPRTALALSLRLPPTVKADGAALALKTLLEKDPPYGAKVSFEYGQAATGWHAPR
                     TVEWLDKALAEASKKHYGKDVMWMGEGGTIPFMAMLGAKFPQAQFLITGVLGPHSNAH
                     GPNEFLHLGYAKKLTACVADVIASFK"
BASE COUNT          240 a          519 c          506 g          240 t
ORIGIN      
        1 gactggccgg cgaggatcat gcgcgagccg tcgacgtagg gcagcgtgcg cgcgtagtca
       61 taggcggcca tcacgtcctg cgaggcggcg ttctccgcct tgcggaagtc ggggttgctg
      121 caggggcccg ggtcctcggc gtagtcgccg ccggtggcgc cgaagccgcg gcgcagcggc
      181 atcaccacca cgtagccgtg ctgcgcgaag accgtggcgg cggcgagaag ctgctcgggg
      241 gcttccttca tgcgctcggc gcgggtgccg gcgacgccgt ggttcagcac caccgcgccg
      301 aagcggccgc tgccttcggg ccggatgatg cgcgtggcga tgctgtagtt gccggccgcg
      361 acggggatac gaacgtcctc ggagacgacc ccgaactgcg cgtaggccgc ttcgccgatg
      421 aagaagctca gcaggcccac cagcgcgccc agcagcgcgc tcctccctaa caaatgcatc
      481 tacgttcacc tcgtcagtta cgcgacgaag tgtagaggat tctgcgatac gcgcaagact
      541 ggaaaaacga cttgcagcgc cgcaaatgcg tcggggcagc ccgacggaga tcttgttatt
      601 taaatgaagc gataacgtcc gccacgcagg ccgtgagctt cttcgcatag ccgaggtgca
      661 ggaactcgtt cggtccgtgc gcgttcgagt gcggcccgag cacgccggtg atgaggaact
      721 gcgcctgcgg gaacttggcg ccgagcatcg ccatgaacgg gatggtgccg ccctcgccca
      781 tccacatgac gtccttccca tagtgcttct tcgaagcctc tgcgagggcc ttgtccagcc
      841 actcaaccgt cctgggagca tgccaacccg tcgcggcctg tccatattca aaggacacct
      901 ttgctccgta gggcgggtcc ttctcgagca gcgtcttcag cgcaagcgcg gcaccgtctg
      961 ccttcaccgt cggcggcagg cgcagcgaga gagcgagcgc ggtcctcggc cgcagcacgt
     1021 tgccggcgtt cgccggcagc ggcaggccct cggcgccggt gacggccagc atcggccgcc
     1081 agctgcggtt cagcaccagg tcggacaggt cctcggccat cggcttcatg ccgccggcga
     1141 acgggaactt gcgccagatc tcctcgccca gcacctgcgc cgcgcgcttc gcctgctcgc
     1201 cacgctcggg cgggatcggc gcgtggaagg cggcgtggcg caccacgccc gtgtcggccg
     1261 cgtcgacgcg ctcgagcagc tcgcgcagga tgcggaagct cgaggccacc acgccgctcg
     1321 cgtcgcccga gtgcaccccc tcggtgagca cctcgacgct cagcacgccg ttcaccaggc
     1381 cgcgcagcga ggtcgtgccc cacagcttct cgtagttgcc gcagcccgag tccaggccga
     1441 tcaccagatc cggggttttg attctcggcg ccagaaattc gaggtatgcg ggcaggtcgt
     1501 agctc
//
LOCUS       DASYJA010000572         2404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1447176, whole genome shotgun sequence.
ACCESSION   DASYJA010000572 DASYJA010000000
VERSION     DASYJA010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447176"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="VGO02_12545"
     CDS             complement(<1..1340)
                     /locus_tag="VGO02_12545"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEV7478553.1"
                     /translation="MSYLVGIDIGGTFTDCAIVDRAGKLLTTKVPSTPHDFSRGMMDA
                     LGAGAQALDLSLERFCRDIDFLSHGTTVGTNTIIQKRGAKVGLITTRGHEDAIHIMRG
                     SRGYGGRDIRKVVHFPETAKPQPIVPKRLIRGVSERVDCFGEVVVELNEKEAQTAIRE
                     LLAEGVQAIAICFLWSFRNPAHEQRVKALVEEISKDLFVTCSVDIAPKWGEYERVTAT
                     ALNAYLGPVMSGYLLRLNNSLENLGYRHGLQIAQCGGGTVPVSRAGEAPLLTLDSGPV
                     AGVTASMFLGAAMGEKHIITTDMGGTSFDVSIIYDGKPAYSFVSNTDQYEYFLPKVDL
                     QAIGAGGGSLVRANPETRTLSVGPDSAGAFPGPVCYGRGGRIPTVTDAQLVLGYLDPE
                     NFAGGRMKLDRQAAFSAIEDLGKKINLTPLETAAGVCRIVELNMADVIRKVTVE"
     gene            1363..1977
                     /locus_tag="VGO02_12550"
     CDS             1363..1977
                     /locus_tag="VGO02_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HEV7478554.1"
                     /translation="MRNAVPVLSLLETRVLGTLVEKQHTVPDTYPLSLNALVSGCNQK
                     TSRSPVMEATQAEVQQALDSLKSANFVMETSGNRVTRFSHNLGRVLQVPAQSEALLTS
                     LMLRGPQTAGELRISSERLHAFSDISAAEGFLNELASRPAGALVVLLPRQPGARESRW
                     AHLLSGTPAIETPDSPREEELSLKSRVERLEGEVAALRDLINRR"
     gene            1974..>2404
                     /locus_tag="VGO02_12555"
     CDS             1974..>2404
                     /locus_tag="VGO02_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478555.1"
                     /translation="MTLKERARAAAELLERLAEDWSQLERLPEEERRRLHLTVARMYQ
                     PDPVARRQRMKAVERERRAAGTRRSEDVLHRTGIRELRRRSVFTTPNVFPPLPAKLLD
                     SPVEGRHCYVCKQKYSAIHHFYDQLCASCAELNFRKRTELA"
BASE COUNT          412 a          813 c          788 g          391 t
ORIGIN      
        1 ttctcgaccg tcaccttgcg gatcacgtcg gccatgttca gctcgacgat gcggcagacg
       61 ccggcggcag tttccagcgg ggtgaggttt attttctttc caagatcttc gattgcggaa
      121 aaagccgcct ggcggtccag cttcatccgt ccgccggcaa aattctcagg atcgagataa
      181 cccagaacca gttgcgcgtc ggtgacagtc gggatcctgc cgccgcgccc gtagcagacc
      241 gggccgggaa aggcgccggc gctgtccggg ccgacgctca gggtgcgggt ctccgggttg
      301 gcgcgcacca ggctgccgcc gccggcgccg atcgcctgca ggtcgacctt cggcaggaag
      361 tactcgtact ggtcggtatt gctgacgaag gagtaggccg gcttgccgtc atagatgatc
      421 gagacgtcga acgaggtgcc gcccatgtcg gtggtgatga tgtgcttctc gcccatcgcc
      481 gcgccgagga acatcgacgc ggtgacgccg gcgaccgggc cggagtcgag cgtcagcagc
      541 ggcgcctcgc cggcgcgtga caccggcacc gtgccgccgc cgcactgggc gatctgcaat
      601 ccatgtctgt atccgagatt ttccaaagaa ttgttaagac gaagcagata gcccgacatc
      661 accggcccca gatacgcatt cagcgcagtc gccgtgaccc gctcgtactc gccccacttc
      721 ggcgcgatgt cgaccgagca ggtgacaaac aggtctttgg agatttcttc gaccagggcc
      781 ttgacccgct gctcatgggc gggattcctg aacgaccaca ggaagcagat cgcgatggcc
      841 tgcacgcctt cggccagcag ctcgcggatc gcggtttgcg cttccttctc attcaattcg
      901 acgaccactt cgccaaagca gtcgacccgt tccgacacgc cgcggatcag gcgcttgggg
      961 acgatcggct gcggcttggc ggtctccggg aagtgcacca ccttgcggat gtcgcgcccg
     1021 ccgtagccgc gcgagccgcg catgatgtgg atcgcgtcct cgtgcccgcg ggtggtgatc
     1081 aggcctacct tggcgccgcg cttctggatg atggtgttgg tgccgacggt agtgccgtgc
     1141 gacaggaaat cgatgtcgcg gcagaagcgc tcgagcgaca ggtcgagcgc ctgcgccccg
     1201 gcgccgagcg cgtccatcat gccgcgcgag aagtcgtgcg gcgtggacgg caccttggtg
     1261 gtgagcagct tgcccgcgcg gtcgacgatg gcgcagtccg tgaacgtgcc gccgatgtcg
     1321 atgccgacca gatagctcat tgcgctatat tagccgcgca ccatgcgcaa cgccgtcccg
     1381 gtcctttctc tcctcgaaac acgcgtcctc ggcaccctcg tcgagaagca gcacaccgtc
     1441 cccgacacgt atccgctcag cctgaacgcc ctggtatccg gctgcaacca gaagaccagc
     1501 cgctcgccgg tgatggaagc gacgcaggcg gaggtgcagc aggccctcga cagcctgaaa
     1561 agtgccaact tcgtcatgga aacgagcggc aaccgggtga cgcgcttttc gcacaacctc
     1621 gggcgggtgc tgcaggtgcc ggcgcagtct gaagccctgc tgacatcgct catgctgcgc
     1681 ggcccgcaga ccgcgggcga gctgcgcatc agcagcgagc ggctgcatgc cttctccgac
     1741 atctccgcgg cggagggttt cctgaacgag ctcgcttccc ggccggccgg cgccctggtc
     1801 gtgctgctgc cccgccagcc gggcgcgcgc gagagccgct gggcgcacct gttgagcggc
     1861 acgcccgcga tcgagacgcc cgacagccct cgggaggaag agctttcact gaaaagcagg
     1921 gtagagcgcc tcgagggcga ggtcgccgcc ctgcgcgacc tgatcaaccg ccgatgacgc
     1981 tcaaggaacg ggcgcgcgcg gccgccgagc ttctcgagcg cctcgccgag gactggagcc
     2041 agctcgagcg cctgccggaa gaagagcgaa ggcgcctgca cctgacagtg gcgcgcatgt
     2101 accagcccga cccagtcgcg cgccgacagc gcatgaaggc ggttgagcgc gagcgcaggg
     2161 cggccggcac ccggcgcagc gaggacgtgc tgcatcgcac cggcatccgc gaactgcgcc
     2221 gcaggtccgt tttcaccacg ccgaacgtgt tcccgccgct gcccgctaag ctgctggaca
     2281 gcccggtgga agggcggcac tgctacgtct gcaagcagaa gtactcggcc atccaccact
     2341 tctacgacca gctttgcgcg tcgtgcgccg agctcaattt ccgcaagcgc accgagctcg
     2401 ccga
//
LOCUS       DASYJA010000573         5257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3083611, whole genome shotgun sequence.
ACCESSION   DASYJA010000573 DASYJA010000000
VERSION     DASYJA010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3083611"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..850)
                     /locus_tag="VGO02_12560"
     CDS             complement(38..850)
                     /locus_tag="VGO02_12560"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEV7478556.1"
                     /translation="MSFSGDERQYLDLLGEVLERGARKTDRTGTGTLSVFGRQLRFAL
                     GASFPLLTTKKLHLKSILYELLWFLRGDTNVKWLQERGVSIWDEWADENGELGPVYGY
                     QWRHWRTPGGGEIDQISKLIENLKARPDSRRHLVTAWNPADVDRMALPPCHALFQFYV
                     SEGELSCQLYQRSADIFLGVPFNIASYSLLTLMVAQVTGLKPGEFVLTLGDAHLYLNH
                     LEQAREQLARAPRPFPRMQLNPARKDLLEFQYEDFTLQAYEPYPAIKAPIAV"
     gene            complement(847..1656)
                     /locus_tag="VGO02_12565"
     CDS             complement(847..1656)
                     /locus_tag="VGO02_12565"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV7478557.1"
                     /translation="MAGAIAKDSKELAPGYRTLHLIGQGGQAQVYLAERVHDGKQLAL
                     KVLDRSLRQDPVFLERFVREYKLLASLENENVARIYDQGFSGDHPYIAMEFLAGGTLA
                     SRIREGIASREALRIVSQIARALDAIHNRGIVHRDLKPANILFRSSGQPVIVDFGLAR
                     DLGINSTLTLAGQVLATPRYMSPEQCVGRTADHRSDLYAVGAIFYEMLTQTKLFGSVT
                     PADLIRMHVSDPIPRLEGPLAGYQTILDRLLAKKPEDRFQNARELFGAIAV"
     gene            1713..3221
                     /locus_tag="VGO02_12570"
     CDS             1713..3221
                     /locus_tag="VGO02_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478558.1"
                     /translation="MLKMFGGGGSDHPMADAKEAKRLLDGLPAQDAKALEELAGWLES
                     VSAAQMKADLRIQRLLAIDEVAQARVRKLARDVLVPSPQVSRFQDNVNWTRLHDYWRH
                     AGLAFARCIDSFLQGAKGAEAAKTLLPLTVARALRCLGQQMKWQLVRYAPADVAIWGV
                     LNKIYAFAELRGITEARVNLYPAGGIESTPRLEFLKVCMMGAASPDSLLPLEVEVAER
                     AIGDLAPSFALAAQPERELLYWTDLALATAPLRSLREPKPAQGLRFFGPGSAVAALAA
                     LIRKMQTTREVPASLSLGGSYDPEVVLSALEHLAVYWDREPPERRHPRHSVKSRLTVT
                     HGFNGVLEALGGNGGSLDFNPAAAENWTVENVSAGGFGVLARQVKSDWLKVGTLLAMQ
                     PDGGPNWVVGAVRRVTRLSKDEARIGIETLSRAPAVSSFSAGAEAQPGVLLAAAPETG
                     ETSIALRAGVFIRGCNLETAVDGRHHVYMPQTVDERGDDYEIARFREMIRES"
     gene            complement(3208..3852)
                     /locus_tag="VGO02_12575"
     CDS             complement(3208..3852)
                     /locus_tag="VGO02_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEV7478559.1"
                     /translation="MDAIEIETGPRPEAAVIWLHGLGADGHDFEPIVPELRLPKAVRF
                     VFPHAPVRPVTINNGMRMRAWYDILQFGGGPEDEQGIRASQALLENLITAQKKKGIQA
                     DKIVLAGFSQGGAIVLQTGLRHTERLAGIMALSTYLPIAETLQKEKSKANQDLPIFMA
                     HGTYDDIIPLRRAEQSRDLLVKSGYPVEWRTYPMPHSVCPEEIGHISEFLLRIL"
     gene            3880..4104
                     /locus_tag="VGO02_12580"
     CDS             3880..4104
                     /locus_tag="VGO02_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478560.1"
                     /translation="MKLSLHATTIVGTIFAAVCLSVAIPGFLSLGDIQDPVQLSDAKG
                     FAWFWAFLAVVALFFAALAQWMARTGQKDE"
     gene            4101..4460
                     /locus_tag="VGO02_12585"
     CDS             4101..4460
                     /locus_tag="VGO02_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478561.1"
                     /translation="MRVKVYALAILAVVSLVVPLLEVMLTGKAEELGTYALAQNLLSI
                     PPIFWWYHVDKAEKGYRAGPLMNAGVVALAILALPVYFIRSRGWKRGSISILKGIVVV
                     AAITLLGMFGEWLGKAW"
     gene            4470..4733
                     /locus_tag="VGO02_12590"
     CDS             4470..4733
                     /locus_tag="VGO02_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478562.1"
                     /translation="MDEKIRIKVTETGQALEVVVFSKRADCIEVVLGEGVHNMKCTLT
                     PTRNGMAYVGNMKGRELVYERSREQVQADIDRLNPNVRGPRRR"
     gene            complement(4735..5112)
                     /locus_tag="VGO02_12595"
     CDS             complement(4735..5112)
                     /locus_tag="VGO02_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478563.1"
                     /translation="MKKSVLVADHSDARLSAILVGHELSFVHTIDEAKAMLARRIFDL
                     IVIGVHFDESRMFDLLRYVRWQERHRATPVVCVLTLDFARHAIAAEGLEIAVKALGGT
                     TYLDLKTIGDGEARHVIEDLLGV"
BASE COUNT          834 a         1678 c         1828 g          917 t
ORIGIN      
        1 cctacaggcg ggcccttttt tttctagacg gccgcagtca taccgctatc ggggccttga
       61 tcgccggata cggttcgtag gcttgcaggg tgaagtcttc gtactggaat tccagcaggt
      121 ctttgcgcgc cggattcagc tgcattcgcg ggaacggccg cggcgcgcgg gcgagctgct
      181 cgcgcgcctg ctcgaggtgg ttcagataca ggtgcgcgtc gcccagggtg agcacgaact
      241 cgccgggctt caggccggtc acctgcgcca ccatcagcgt gagcagcgaa taggaggcga
      301 tgttgaacgg cacgccgagg aaaatgtccg cgctgcgctg gtagagctgg cagctcagtt
      361 cgccctcgct cacgtagaac tggaacagcg cgtggcaggg cggcagcgcc atgcggtcga
      421 cgtccgctgg gttccaggcc gtgacgaggt ggcggcggga atccggcctt gctttcagat
      481 tttcaatcag tttggaaatc tgatcgattt ctcctccgcc tggcgtgcgc cagtggcgcc
      541 actggtagcc gtacaccggg ccgagctcgc cgttctcgtc ggcccactcg tcccagatgc
      601 tgacgccgcg ctcctgcagc cacttcacgt tggtgtcgcc gcgcaggaac caaagcagct
      661 cgtagaggat cgacttgagg tgcagcttct tggtggtgag cagcggaaag ctcgcgccga
      721 gcgcgaagcg cagctgcctg ccgaacacgg aaagcgtccc ggtgccggtg cggtcggtct
      781 tgcgggcgcc gcgctcgagg acttccccga gcagatcgag gtactgccgc tcgtcgccgc
      841 tgaaactcaa accgctatgg cgccgaacag ctcgcgcgcg ttctggaagc gatcctcggg
      901 tttcttcgcc agcagccggt cgaggatcgt ctggtagccg gcgagcggcc cctcgaggcg
      961 agggatcggg tcgctgacgt gcatgcggat caggtccgcc ggggtcacgc tgccgaagag
     1021 cttggtctgg gtgagcatct cgtagaagat cgcgcccacc gcgtagagat ccgagcggtg
     1081 gtcggcggtg cgccccacgc actgctcggg gctcatgtag cgcggcgtgg cgagcacctg
     1141 gccggcgaga gtcagcgtcg agttgatgcc gaggtcgcgc gccaggccga agtcgacgat
     1201 caccggctgg ccgctggagc ggaacaggat gttcgccggc ttcaggtcgc ggtgcacgat
     1261 gccgcggttg tggatcgcgt cgagcgcgcg cgcgatctgc gacacgatgc gcagcgcttc
     1321 gcgcgaggcg atgccctcgc ggatgcggct ggcgagcgtg ccgccggcga ggaactccat
     1381 cgcgatgtac ggatggtcgc ccgagaagcc ctggtcgtag atgcgcgcca cgttttcgtt
     1441 ctcgaggctc gccagcagct tgtactcgcg cacgaagcgc tcgaggaaca ccgggtcctg
     1501 gcgcagcgag cggtcgagca ctttgagcgc gagctgcttg ccgtcgtgca cgcgctcggc
     1561 gagatacacc tgggcctgcc cgccctggcc tatgaggtgc agcgtgcggt agcccggcgc
     1621 cagttcctta gaatcttttg cgattgcccc ggccatctgg gaccgtattg tgccacttca
     1681 ccatgctacg atttcggaca aaggggtaaa tcatgctgaa gatgttcggt gggggagggt
     1741 ccgaccatcc gatggccgac gcgaaggagg cgaagcgcct cctggacggg ctgccggcgc
     1801 aggatgccaa ggcactcgag gaactggcgg gctggctcga gtcggtgagc gcggcgcaga
     1861 tgaaggccga cctgcgcatc cagcgcctgc tcgcgataga cgaagtggcg caggcccgcg
     1921 tgcgcaagct ggcgcgcgac gtgctggttc cgtcgccgca ggtttcgcgc tttcaggaca
     1981 acgtcaactg gacgcgcctg cacgactact ggcgacatgc cggcctggct ttcgcccgct
     2041 gcatcgacag ctttctgcaa ggcgccaagg gcgccgaggc cgcgaagacg ctcctgccgc
     2101 tgacggtggc gcgcgcgctg cgctgtctcg gccagcagat gaagtggcag ctcgtgcgct
     2161 acgcgccggc cgatgtcgcc atctggggcg tgctcaacaa gatctatgcg tttgccgagc
     2221 tgcgcggcat caccgaagcc cgcgtcaacc tgtacccggc cggcggcatc gaatcgacgc
     2281 cgcgcctgga gttcctcaag gtttgcatga tgggcgccgc ctcgcccgac agcctgctgc
     2341 cgctcgaggt cgaggtggcg gaacgggcga tcggcgatct cgcgccgagc ttcgcgctcg
     2401 ccgcgcagcc cgagcgcgag ctgctctact ggaccgacct cgcgctcgcg acggcgccgc
     2461 tgcgctcgct gcgcgagccg aagcccgcgc aaggcctgcg cttcttcggc ccgggcagcg
     2521 cggtggccgc gctcgccgcg ctgatccgca agatgcagac gacgcgggaa gtgcccgcca
     2581 gcctcagcct gggcggcagc tacgatcccg aggtggtgct cagcgcgctc gagcacctgg
     2641 cggtgtactg ggaccgcgag cctcccgagc gccggcatcc gcgccacagc gtgaagtcgc
     2701 gcctgaccgt gacgcacggc ttcaacggcg tgctcgaggc gctcggcgga aacggcggtt
     2761 cgctcgactt caaccccgcc gcggcggaga actggacggt cgaaaacgtc agcgccggcg
     2821 gcttcggcgt gttggcgagg caggtgaaga gcgactggct caaggtcggc acgctgctcg
     2881 ccatgcagcc cgacggcggt cccaactggg tggtcggcgc ggtgcgccgc gtcacccggc
     2941 tgtcgaagga cgaggcgcgc atcggcatcg agacgctgtc gcgcgcgccg gccgtgtcga
     3001 gcttctcggc cggcgcggag gcgcagccgg gggtgctgct cgccgcggcg cccgagaccg
     3061 gcgaaacctc gatcgcgctg cgcgccgggg tgttcatccg cggctgcaac ctcgagaccg
     3121 cggtcgacgg ccggcaccac gtctacatgc cgcagacggt ggacgagcgc ggcgacgact
     3181 acgagatcgc ccgcttccgc gagatgatca gagaatcctg agcaggaact cggagatgtg
     3241 cccgatctcc tccgggcata cggagtgcgg catcgggtag gtccgccact cgaccgggta
     3301 gccgcttttc accagcaggt ccctcgattg ctccgcgcgg cgcagcggaa tgatgtcgtc
     3361 gtaggtgccg tgcgccatga agatcggcag atcctggttg gctttgcttt tttctttctg
     3421 cagcgtttct gcaatcggca agtaggtaga tagcgccatg atccccgcca ggcgctcggt
     3481 atggcgcagg ccggtctgca gcacgatggc gccaccctgc gagaagccgg ccaggacgat
     3541 cttgtcggct tggatgcctt ttttcttctg tgcagtgatg agattctcaa gcaatgcctg
     3601 agacgccctg atcccctgct cgtcctccgg cccgccgccg aactggagga tgtcgtacca
     3661 cgcgcgcatg cgcatgccgt tgttgatggt gaccgggcgc accggggcgt gcggaaagac
     3721 gaagcgcacc gccttgggca ggcgcagctc cgggacgatg ggctcgaagt cgtggccgtc
     3781 ggcgcccagg ccgtgcagcc agatcaccgc cgcttccggg cgcggcccgg tttcgatctc
     3841 gatcgcatcc attttgcccg cagtgtaatc tagccgtgag tgaagctctc gctgcacgcc
     3901 accaccatcg tcggcacgat cttcgcggcc gtctgcctga gcgtcgcgat ccccggcttt
     3961 ctctccctcg gcgacatcca ggacccggtc cagctttccg acgcgaaggg cttcgcatgg
     4021 ttctgggcgt tcctcgccgt cgtggcgctg ttcttcgcgg cgctggcgca gtggatggcg
     4081 cgcaccgggc agaaggatga atgagggtca aggtctatgc cctcgccatc ctggcggtgg
     4141 tttcgctggt cgtgcccctg ctcgaggtga tgctgaccgg caaagccgag gagctcggca
     4201 cgtacgcgct ggcgcagaac ctgctctcga tcccgccgat cttctggtgg tatcacgtcg
     4261 acaaggctga aaagggatac cgcgccgggc cgctgatgaa cgcgggcgtg gtggcgctgg
     4321 ccatcctcgc gctgccggtc tacttcatcc gctcgcgcgg ctggaagaga ggatcgattt
     4381 cgatcctgaa gggcatcgtc gtcgtggcgg cgatcacgct tctcggcatg ttcggtgagt
     4441 ggctcggcaa ggcgtggtag tgtccgccga tggacgagaa aatcaggatc aaggtgaccg
     4501 agaccggcca ggcgctcgag gtggtggtgt tcagcaagcg cgccgactgc atcgaggtag
     4561 tcctcggcga gggcgtgcac aacatgaagt gcaccctgac gccgacgcgc aacgggatgg
     4621 cgtatgtcgg caacatgaag gggcgcgagc tggtctacga gcgcagccgc gagcaggtgc
     4681 aggctgacat cgaccgcctc aacccgaacg tgcgcgggcc gcgacggcgc taggtcagac
     4741 gcccagcagg tcctctatga cgtggcgcgc ctcgccgtcg ccgatggtct tcagatccag
     4801 gtaggtggtg ccgcccagcg ccttgacggc gatctcgagc ccttcggctg cgatcgcgtg
     4861 gcgcgcgaag tcgagggtca gcacgcagac caccggcgtg gcgcggtggc gctcctgcca
     4921 gcgcacgtag cgcagcaggt cgaacatgcg cgattcgtcg aaatgcacgc cgatgacgat
     4981 gagatcgaag atgcggcggg cgagcatcgc cttcgcttcg tcgatggtgt gtacgaagct
     5041 cagctcatgg ccgacgagga ttgcggaaag ccgcgcatcc gagtggtcgg ccaccaggac
     5101 agactttttc atcgggacaa ctgtcatggt aatcagccat gcatgtgtcc cgttccatac
     5161 gacagttgat ccggccggat tacttctttc ggagggctga cttcgggcgg aagaccttga
     5221 ccacggtctc gtgggtctcg atgtaggggc cgccgat
//
LOCUS       DASYJA010000574         4486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1993226, whole genome shotgun sequence.
ACCESSION   DASYJA010000574 DASYJA010000000
VERSION     DASYJA010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1993226"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1087
                     /locus_tag="VGO02_12600"
     CDS             134..1087
                     /locus_tag="VGO02_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEV7478564.1"
                     /translation="MRYQLKPIYCRPWTLNGLSLQLIESHYENNYGGALRRLNAIAAQ
                     IESLDPAKTPGYLLNGLKREELIALNSTLLHELYFASMGGDGKPTAPLVEALARDFGS
                     LDRWRDEFVAMANALGGGSGWVVLVYLPRDRRLVNQYASDHSQSVAGGVPILALDMYE
                     HAYHIDFGANAKAYVDAFMRNIDWLAVQGRYEDASKVEPPRPLVQKEFGDLPGVAVEE
                     VKAMIDAGKAVQFIDARPRHYVSRTRDIVEGATWRDPERVQEWMGELSKSDPVVVFCV
                     YGFHVGCRTAITLREAGFDAKYMKGGHSGWKALGGPVRMQT"
     gene            1048..2280
                     /gene="chrA"
                     /locus_tag="VGO02_12605"
     CDS             1048..2280
                     /gene="chrA"
                     /locus_tag="VGO02_12605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEV7478565.1"
                     /translation="MEGPGRAGPDADVKAAGIPAYPKNLKEIASAFLLLGTTFGGPAI
                     MGIMQAEIQEKRQWVTKARLVEGLSLVTALPGATALQLGIFLGYNRGGWWGGLIAGLC
                     FVLPAFFIMLALAAGYAAYGALPLMRGIVYGLGPVVVGVFLVAVYRLGRAVAVTTSQR
                     VVAIASATALALTPLGVVAILALGGAIGLVLFHSRRLGAIVVCGVCALIALVHFSPWS
                     VTALVPAALDAGTSARPASLLDIGSFFLMTGALSFGGGLSLLAFIQQHVVNHYHWLTP
                     EEFVAGLALGQLTPGPIIMVAAYVGYKLAGMAGAALATAAVFLPSFVLMLSILPFFDR
                     ATKVLWTKAAMKGIGPAVLGVLAVSLLQVASHALRDLPAVVLFVATIAALLAWRVGVF
                     KLMAAGAVLGALYASLVG"
     gene            2285..3454
                     /locus_tag="VGO02_12610"
     CDS             2285..3454
                     /locus_tag="VGO02_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7478566.1"
                     /translation="MIRLVLTARALRGFADGCIAVVLPAYLLALGHGDLEVGLISTAT
                     LFGSSFTTLAVGRWGHRFSIRRLLLGVALLMAATGAAFAGFSSLWPLLVVGFVGTLNP
                     SSGDASVFMPLDHTMLATAPAEQRTTLFARYSFLGSIFGALGALAAGVPEWLAAAGWS
                     MVEALRAMFVLYGAIGIAVWLLYLRLPNPRTEEHRLAVPLGPSRGIVWRLAGLFSVDS
                     FAGGFTLNSLLALWLFQRFDMSLASAGAFFFWTGLLAAVSQLAAAPLARRIGLINTMV
                     FTHIPSSLFLIGAALASSLELTLALLLARSLLSQMDVPARGAFVMSVVTPEERPAAAS
                     FTAVPRSLAAAVSPTLTGAMFAAGAMIAPLVICGVLKIAYDLALLTAFRRVKPRS"
     gene            complement(3420..3788)
                     /locus_tag="VGO02_12615"
     CDS             complement(3420..3788)
                     /locus_tag="VGO02_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478567.1"
                     /translation="MKRNFLSIVVTSALAALPVASFAQTSGDTADKGGSQAVSPAAPA
                     GSTASSYGSSPRCDAMTGTEKEQCLRDEAEKTQNSQPDDKTPQGNAGSGSTSPAAPAA
                     GDDAAKPSSSTTEASPAEKQ"
     gene            complement(3877..>4486)
                     /locus_tag="VGO02_12620"
     CDS             complement(3877..>4486)
                     /locus_tag="VGO02_12620"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV7478568.1"
                     /translation="LQPHGPCLYRKGMSAKQPLSRGVHHLALNTDDMKMTLDFYTRVL
                     GMPLVHALKVPPGIGTGPGNRGNPPFENLRHYFLDAGGDSLLAFFEIPKGKTPKADRN
                     AIGTMQHVSFAVSEERFSQVKSNVEKAGLSYLGPINVGVETWSIYFFDPNGIRLEFSH
                     QKEADPQVVKRWTQTKAEALAELRTLSQDQAWLDGMVAHLPE"
BASE COUNT          640 a         1427 c         1538 g          881 t
ORIGIN      
        1 cgggaaggcc tacatcgacg tccaggataa acggatcagc ggcagcggag cggtaggtat
       61 gtggaccaag gccgacagcg tcactgcgtt cgacgacttc agcttcggct cggcgaaata
      121 acggaggcct gacatgcgat accagctcaa acccatctac tgccgtccct ggaccctgaa
      181 cggcctttcg cttcagctca tcgagagtca ctacgaaaac aactacggcg gcgccctacg
      241 acgcctgaac gcgatcgccg cgcagatcga atcgctcgat cccgcgaaga cgccgggcta
      301 cctgctgaac ggcctgaagc gcgaggagct catcgcgctg aattccacgc tgctgcatga
      361 gctgtatttc gcgagcatgg gcggggatgg caagccgacc gcgccgctcg tcgaggcgct
      421 ggcgcgcgat ttcggctcgc tcgaccgctg gcgcgacgaa tttgtggcaa tggcgaacgc
      481 cctggggggc gggtcgggct gggtggtgct ggtctacctg cccagagacc gccggctcgt
      541 gaaccagtac gcctcggacc acagccagtc ggtggcaggc ggagtaccga tcctcgcact
      601 cgacatgtac gagcacgcct atcacatcga cttcggcgcc aatgcgaagg cctacgtgga
      661 cgccttcatg cggaacatcg actggctggc ggtgcagggc cggtatgagg acgcgtcgaa
      721 agtcgagccg ccgcggccgc tggtgcagaa ggagttcggc gacctgcccg gcgtcgcggt
      781 ggaggaggtc aaggcgatga tcgatgccgg gaaggcggtg cagttcatcg atgcgcgacc
      841 gcgccactat gtttcccgca cgcgggacat cgtcgaaggc gccacgtggc gggaccccga
      901 acgcgtgcag gagtggatgg gtgagctgtc caaatcggac ccggtggtgg tgttctgcgt
      961 ctacggcttc cacgtcggct gccgcaccgc gatcacgctg cgcgaggcgg gcttcgatgc
     1021 gaagtacatg aagggcggcc actccggatg gaaggccctg ggagggccgg tccggatgca
     1081 gacgtgaagg cggcggggat tccggcgtac ccgaaaaacc tgaaggaaat cgcctccgcg
     1141 ttcctgttgc tcggcaccac cttcggcggc cccgccatca tggggatcat gcaggcggag
     1201 atccaggaga agcgccagtg ggtgacgaaa gcgcgcctcg tcgaaggcct gtcgctggtg
     1261 acggccctgc cgggggccac cgcgctgcag ctcggcatct tcctgggcta caaccgggga
     1321 ggctggtggg gcggcctgat cgcgggactg tgcttcgtgc tgccggcgtt cttcatcatg
     1381 ctcgcgctcg ctgcgggcta cgcggcctac ggcgcgttgc cgctgatgag aggcatcgtg
     1441 tacgggctgg gccccgtcgt cgtgggcgtg ttccttgtcg ccgtataccg gctgggcagg
     1501 gcggtggcgg ttactacttc gcaaagggta gtggcgattg catcggcaac ggctcttgcc
     1561 ttgactccgc ttggcgtagt cgcgatcctc gcgctgggcg gggcgatcgg gctggtgctg
     1621 ttccactccc gcaggctagg tgcgatcgtt gtctgcggtg tctgcgcgct tatcgccctc
     1681 gtgcattttt cgccatggtc ggttacggcg ctcgttccgg ctgcgctcga cgccgggact
     1741 tcggcgcgcc cggcaagcct gctcgacata gggtcgttct tcctcatgac gggggcactc
     1801 tcgttcggcg gcggcctgtc gttgctcgcg ttcatacagc agcacgtggt caaccactac
     1861 cattggctca cgccggagga gttcgtggcg ggacttgccc tgggccaatt gacgcccggg
     1921 ccgatcatca tggtcgcggc gtatgtgggg tacaagctgg ccggcatggc gggcgcagcg
     1981 ctcgccaccg ctgcggtctt tctgccttcg ttcgtcctca tgctttcgat tcttccgttc
     2041 ttcgacaggg cgacgaaggt gctatggacc aaggcggcga tgaagggaat cggccctgcc
     2101 gttctcggcg ttctggccgt ctccctgctg caggtcgcgt cgcatgcgct gcgtgatctt
     2161 cccgcggtgg ttctcttcgt agcgaccata gctgcgcttc ttgcctggcg ggtcggggtt
     2221 ttcaagctca tggcagccgg cgccgtcctg ggggcgctgt acgcctcctt ggtaggatga
     2281 gtcgatgatc cgtctcgtcc tgacagcgag agcgctgcgc ggattcgccg acggctgcat
     2341 tgccgtcgtg ttgcccgcct acctgcttgc gctcgggcac ggcgacctcg aggtcgggct
     2401 gatcagcacc gcgacgctct tcggctcttc cttcaccacc ctcgcggtgg gacgctgggg
     2461 gcatcgcttc tcgatccggc ggctgctgct cggggtagcg ctgctcatgg cggcgacggg
     2521 agccgcattc gcgggattct cctcgctgtg gccgctgctg gtggtgggct ttgtcggcac
     2581 gctcaatccg agcagcggcg atgccagcgt cttcatgccg ctggatcaca cgatgctggc
     2641 cacggcgccc gccgagcagc gcactacgct attcgcgcgc tacagcttcc tcggctcgat
     2701 attcggcgcc ctgggtgcgc tggctgcggg cgttccggaa tggcttgcgg ctgccggatg
     2761 gtcgatggtc gaggccttgc gggcgatgtt cgtgctctat ggcgcgatcg gaatcgcggt
     2821 atggctgctc tatctgcgcc tgccgaatcc gcgcaccgag gaacacaggc tggcagtccc
     2881 cctggggcct tcgcgcggta tcgtgtggcg gctggccggt ctgttcagcg tggactcctt
     2941 tgccggcggg ttcaccctga actcgctcct ggcgctgtgg ctcttccagc gcttcgacat
     3001 gagcctcgcg tcagccggcg cattcttctt ctggaccggg ctgctggcgg cggtatcgca
     3061 gctcgcggcg gcgccgctcg ccaggcggat cggcctcatc aacaccatgg tgttcacgca
     3121 catcccgtcc agcctgttcc tcatcggggc ggcgctcgcc tccagcctcg agctgacgct
     3181 cgccctcctg ctggcgcgca gccttctctc gcagatggac gtgccggcgc gcggcgcctt
     3241 cgtcatgagc gtcgttactc ccgaagagcg gccggcggcc gcgagcttca ccgccgtgcc
     3301 gcgcagcctg gcggcggcag tcagcccaac gctgactggc gcgatgttcg ccgccggcgc
     3361 aatgatcgcg ccgctggtga tctgcggcgt gctgaagatc gcctacgacc tcgcgctgct
     3421 cactgctttt cggcgggtga agcctcggtc gtagaagagg acggtttcgc agcatcgtcg
     3481 ccggccgcag gcgcggcggg cgaagtactt ccgctgccgg cgttaccttg cggggtcttg
     3541 tcgtcgggct gactgttctg cgtcttctcc gcctcgtcgc gcaggcattg ctccttctcg
     3601 gtgccagtca tggcgtcgca gcggggcgag ctgccgtagc tgctcgccgt cgatccggcc
     3661 ggcgcggcgg gtgagacggc ctgcgagcca cctttgtccg cggtgtcgcc ggatgtctgc
     3721 gcgaacgatg cgacgggcag ggcagcgagc gcggaggtca cgacgatgct cagaaagttc
     3781 cttttcattt gcttctcctt tcgtggaatc acgaaccaag gagcaagcga agtgcctgtc
     3841 aacctgccga cagctagcgg gcagcgcccg cgaggtttat tccggcaggt gggcgaccat
     3901 cccgtcgagc caggcctggt cctgcgaaag cgtacgtagc tcggccagcg cctcggcctt
     3961 ggtctgcgtc cagcgcttca ccacctgcgg gtcggcttcc ttctggtgcg agaactccag
     4021 gcggatgccg ttcgggtcga agaagtagat cgaccaggtc tccacgccga cgttgatcgg
     4081 gccgaggtac gaaagcccgg ctttttcaac gttgctcttc acttgggaaa atctctcctc
     4141 ggaaaccgca aacgagacgt gctgcatggt tccgatcgcg ttgcggtcgg ccttgggcgt
     4201 cttgcccttc ggaatctcga agaaggcgag gagggaatcg ccgccggcgt cgaggaagta
     4261 gtggcgcagg ttctcgaacg gcgggttgcc gcggttcccc ggcccggtgc cgatgcccgg
     4321 cggcaccttg agcgcgtgca ccagcggcat gccgaggacg cgcgtgtaga agtcgagcgt
     4381 catcttcatg tcgtcggtat ttagcgcgag gtgatgaacc ccgcgcgaga gcggctgctt
     4441 tgcgctcatg cctttccggt atagacaggg cccgtgcggc tgcagc
//
LOCUS       DASYJA010000575         1898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2174743, whole genome shotgun sequence.
ACCESSION   DASYJA010000575 DASYJA010000000
VERSION     DASYJA010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2174743"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VGO02_12625"
     CDS             complement(<1..579)
                     /locus_tag="VGO02_12625"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:TIGR01854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEV7478569.1"
                     /translation="MGAGVPALFISDLHLSEDRPAANERFFSFLEDTARSADALYILG
                     DFFEYWIGDDDLAYPFNAVVAGALRELTRSGVALYLMHGNRDFLIGAGFCEATGGSLL
                     EDPTVVELGGEKTLLAHGDTLCTDDLDYQAWRRTARSAAWQREFLGKSLAERRRAVGG
                     MREKSKEVIQAKPAEIMDVNAGAVRSAMEQHAV"
     gene            complement(580..1128)
                     /locus_tag="VGO02_12630"
     CDS             complement(580..1128)
                     /locus_tag="VGO02_12630"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV7478570.1"
                     /translation="MVPSGALAADPQVDLRTSVGTIRLELYPAKAPKTVENFLQYVRD
                     GHYNGTVFHRVIDGFMVQGGGFEASYKQKPTRKPVANEAKAGVNAGLKNEVGTVAMAR
                     TSDPDSATAQFFINVGDNAFLNWGDSKGDGVGYTVFGKVVSGMDVVTKIAKTPTGSGG
                     PFQRDVPRTAVVIEQASVVAAK"
     gene            complement(1164..>1898)
                     /locus_tag="VGO02_12635"
     CDS             complement(1164..>1898)
                     /locus_tag="VGO02_12635"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEV7478571.1"
                     /translation="GGRPRLIADYYVTLGKNGMEKTRQGDQKTPVGVYHVTANLPRKK
                     LTDFYGAGAFPINYPNEWDKRQGRNGFGIWLHGVPSDVYSRAPRASDGCIVLANPDLE
                     SVGRYVQVGLTPVVIADEVEWAEASAVEAERASLAAALERWRADWESRDTERYLAHYS
                     ARFAAPGQNLASWSAHKRNVNGAKRWIKVGLSKVAMIRYPRERDFVVVTFDQDYRSDG
                     LSNVMHKRQYWVKEDGNWKIIYEGAG"
BASE COUNT          290 a          629 c          639 g          340 t
ORIGIN      
        1 cactgcgtgc tgctccattg ccgagcgcac cgcgccggca ttgacgtcca tgatctcggc
       61 cggcttcgcc tggatcactt ccttgctttt ctcgcgcatg ccgccgactg cgcggcggcg
      121 ctcggcgagg gatttgccga ggaattcccg ctgccaggcg gccgagcgcg cggtgcgccg
      181 ccatgcctgg tagtcgagat cgtcggtgca cagcgtgtcg ccatgcgcca gcagggtttt
      241 ctcgccgccg agctccacca cagtcgggtc ctcgaggagg ctgccgccgg tcgcctcgca
      301 aaagccggcg ccgatgagga aatcccggtt gccgtgcatc aggtagagcg ccacgccgct
      361 gcgcgtcagc tcgcgcagcg ccccggctac gaccgcgttg aaaggatagg cgagatcgtc
      421 gtcgccgatc cagtactcga agaagtcgcc gaggatgtag agcgcgtcgg cgctgcgcgc
      481 ggtgtcctcg agaaaggaga agaaacgctc gttggcggcc ggccggtctt cgctgaggtg
      541 cagatccgag atgaataacg ccggcacgcc ggcacccacc tatttcgcgg ctacgacact
      601 cgcctgctcg atgaccacgg cggtgcgcgg cacgtcgcgc tggaagggcc cgcccgaccc
      661 ggtcggggtc ttggcgatct tggtcaccac gtccatgccg gaaacgacct tgccaaacac
      721 cgtatagccg acgccgtcgc ccttggagtc gccccagttg aggaaggcgt tgtcgccgac
      781 gttgatgaag aactgcgcgg tggccgaatc cgggtcggag gtgcgggcca tggcgacggt
      841 gccgacctcg ttcttcaggc cggcgttcac gcccgccttg gcttcgttgg cgaccggctt
      901 gcgcgtcggc ttctgcttgt agctcgcctc gaagccgccg ccctggacca tgaagccgtc
      961 gatcacgcga tggaacacag tgccgttgta gtggccgtcg cgcacgtact gcaggaagtt
     1021 ctctactgtt ttcggcgcct tggccgggta cagctcgagg cggatcgtac cgacgctggt
     1081 gcgcaggtcg acctggggat cggcggcgag cgcgccggac gggaccaccg cagcggtcga
     1141 gagtatgaaa agacgcgtca ggttcaacca gccccctcgt agatgatttt ccagttgccg
     1201 tcttccttga cccagtactg ccgcttgtgc atgacattgg acagcccgtc cgagcggtag
     1261 tcctggtcga aagtgacgac gacgaaatcg cgctcgcgcg ggtagcggat catcgccacc
     1321 ttcgacagcc cgaccttgat ccagcgcttc gcgccgttga cgttgcgctt gtgggcgctc
     1381 cacgacgcca ggttctggcc cggcgcggcg aagcgcgccg agtagtgcgc gagatagcgc
     1441 tcggtgtcgc ggctttccca gtcggcgcgc cagcgctcga gcgcagcggc gagtgaagcg
     1501 cgctcggcct cgacggccga agcctcggcc cactcgacct cgtcggcgat cactaccggc
     1561 gtgagtccca cctgcacata gcggccgacc gattccaggt ccggattggc gagcacgatg
     1621 cagccgtcgc tcgcgcgcgg cgctcggctg tagacgtcgg agggcacgcc gtgcagccag
     1681 atgccgaagc cgttgcggcc ctggcgcttg tcccattcgt tcggatagtt gatcgggaag
     1741 gcgcccgcgc cgtagaaatc ggtgagcttc ttgcgcggca ggttcgccgt gacgtgatag
     1801 acgccgaccg gcgtcttctg gtcgccctgg cgtgtcttct ccatgccgtt cttgccgagc
     1861 gtgacgtagt agtcggcgat cagccgcgga cggccgcc
//
LOCUS       DASYJA010000576         2015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3175368, whole genome shotgun sequence.
ACCESSION   DASYJA010000576 DASYJA010000000
VERSION     DASYJA010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3175368"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VGO02_12640"
     CDS             complement(<1..543)
                     /locus_tag="VGO02_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478572.1"
                     /translation="MLSSKWCAAAFAAVTCAAQAQQSDQVKQLEERVQQLEQRVQEGQ
                     AQAPARQQSENAMNPAISAILNGVYSNLKLDPNTYRINGFVPTMGDVAPPPRGFSLGE
                     SELGISANVDHLFRGVLIASISPDDNSIGVEEGYIQTLGLSHGFSVKAGRFFSAVGYQ
                     NQIHAHAWDFTDAPLVMKAFL"
     gene            complement(603..896)
                     /locus_tag="VGO02_12645"
     CDS             complement(603..896)
                     /locus_tag="VGO02_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478573.1"
                     /translation="MLAVALLAAWQSALVHPLKHSDGRGGFVHLADGHDHGKRSNTSG
                     LCDALAALTACAPGSLQFFDAPVPSKAVLVVRHGAPRIAEAPPFLSQGPPAVL"
     gene            complement(928..1389)
                     /locus_tag="VGO02_12650"
     CDS             complement(928..1389)
                     /locus_tag="VGO02_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HEV7478574.1"
                     /translation="MKRLLLLLVALALPAVAQQQQPPGADPNFKPLPERKDVVSWKLL
                     AQVELVKMKDRYVPQFATGVSSLDQKQVKVQGFMMPLEMGDKQTHFVLASMPQTCAFC
                     MPGGPESMVEVKTKTPVKYTFDAVVVSGKLTVLKDDPTGIFYRLTEAEPAK"
     gene            complement(1430..>2015)
                     /locus_tag="VGO02_12655"
     CDS             complement(1430..>2015)
                     /locus_tag="VGO02_12655"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV7478575.1"
                     /translation="WEVHEHGQGAKGKEHEVTVVLVQYASPLAAAVLPRQINANSELQ
                     AASPAYETARLFRIVGAGVEALRAFAAVLMLAAGLSVFIALYTALEERRYDLAVMRTL
                     GASPARLFGLLLAEGLMLSLVGAVLGVALGHGLAAALGLWLDAQQQYPVSGLVFRVEE
                     LWIVAVALAVGLAAALLPAWRAYRTDVAGTLSRG"
BASE COUNT          347 a          645 c          700 g          323 t
ORIGIN      
        1 gaggaatgcc ttcatcacca gcggcgcgtc ggtgaagtcc caggcgtggg cgtggatctg
       61 gttctggtag cccactgccg agaagaaccg gcccgccttg acgctgaagc cgtgggacag
      121 gccgagagtc tggatgtagc cttcctcgac gccgatcgag ttgtcatccg gcgagatcga
      181 cgcgatcagc acgccgcgga agagatgatc gacgttcgcc gagatgccga gctcggattc
      241 gcccaggctg aagccccgag gcggcggcgc cacgtcgccc atcgtcggca cgaagccgtt
      301 gatgcggtag gtgttcggat ccagcttgag gttggagtag acgccgttga gaatcgccga
      361 aatcgccgga ttcatggcgt tttccgactg ctgtcgcgcc ggcgcctgag cctggccctc
      421 ctgcacgcgt tgctcgagct gttgcacgcg ctcctcgagc tgcttcacct ggtcggattg
      481 ctgtgcttgt gctgcgcaag tcaccgcggc gaacgccgcg gcgcaccact tgctgctaag
      541 catggttgtc ctctctgaac gattacagat ggcgcgccca tcggcgcgcg gtcagatcgc
      601 tgctagagga cggcgggcgg gccctgggaa aggaacggcg gcgcttccgc aatgcgcggt
      661 gcgccgtgac gcacaacgag gactgctttg gaaggaaccg gtgcgtcgaa gaactgcagc
      721 gaccccggtg cacaggcggt cagcgccgcg agcgcatcgc acagaccgga ggtattggac
      781 cgcttcccgt ggtcatggcc gtcggcgagg tggacgaagc cgccgcggcc gtccgagtgt
      841 tttagcgggt ggacgagcgc gctctgccat gctgccagca gcgcgactgc aagcaatagc
      901 cgcgccagca ggcgcgagaa tgaagtgcta cttggcaggc tcggcctcgg taaggcggta
      961 gaagatgccg gtcgggtcgt ccttgaggac ggtgagcttg ccgctcacca cgaccgcgtc
     1021 gaaggtgtat ttcaccggcg tcttggtctt gacctcgacc atgctctccg gaccgccggg
     1081 catgcagaag gcgcaggtct gcggcatcga ggcgagcacg aagtgcgtct gcttgtcgcc
     1141 catctcgagg ggcatcatga agccctgcac cttgacctgc ttctggtcga gcgagctgac
     1201 gccggtagcg aactgcggca cgtagcggtc cttcatcttc accagctcca cctgcgccag
     1261 cagcttccag gagacgacgt ccttgcgctc cggcagcggc ttgaagttcg gatccgcgcc
     1321 cggcggctgc tgttgctgcg ccacggcggg aagagcaagc gcgacgagga ggaggagaag
     1381 tcgtttcacg ggatgcagag tatttgccga ttcgcgtcgc tttgcaatat cagcctctgg
     1441 aaagggtccc ggcgacatcg gtccggtaag cgcgccaggc gggcagcagg gccgccgcca
     1501 gccccacggc cagggcgacg gccacgatcc agagctcctc gacccggaaa acgaggcccg
     1561 acaccgggta ttgctgctgc gcatcgagcc agaggccgag cgcggcggcg aggccgtggc
     1621 cgagcgccac cccgagcacg gcgccgacga gcgagagcat cagcccctcg gcgagcagca
     1681 ggccgaagag ccgcgccggc gaagcgccga gcgtgcgcat cacggcgagg tcgtagcggc
     1741 gctcctcgag cgcggtgtag agcgcgatga acacggagag gcctgctgcg agcatcagca
     1801 cggcggcaaa ggcgcgcagc gcctcgacac cagcgcctac gatgcggaac aggcgcgcgg
     1861 tctcgtatgc cggcgaagcc gcctgcagct cgctgttcgc gttgatctgc cgcggcagca
     1921 ccgcggcggc gagcggcgag gcgtattgca cgagcaccac ggtgacctcg tgctctttgc
     1981 ccttggcacc ctgaccgtgt tcatgcacct cccag
//
LOCUS       DASYJA010000577         5247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_268446, whole genome shotgun sequence.
ACCESSION   DASYJA010000577 DASYJA010000000
VERSION     DASYJA010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268446"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VGO02_12660"
     CDS             complement(<1..484)
                     /locus_tag="VGO02_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEV7478576.1"
                     /translation="MKKKLPAAVWILVALVLGIIVGYMIFVSFPDKKSAAQIAGYVSI
                     LADVFLRLIKMLIGPLVFSTLVVGIAHMGDAASVGRVFAKSLGWFITASLVSLVLGLI
                     LANLLQPGHNLGLPLPDIGSSANLATAKFTLKDFVSHLVPKSFAEAMANNEILQIVVF
                     S"
     gene            complement(525..1097)
                     /locus_tag="VGO02_12665"
     CDS             complement(525..1097)
                     /locus_tag="VGO02_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478577.1"
                     /translation="MSAAFWLVKWIALLFSLCLMAGTAGAEEMKMHRKQAGEPDKSGW
                     MLAKSTEGRFSVRLPLKFNDFTVTEADPNAPAARTYTVGARSSERIAVVATRIAYRKG
                     AAAAKEYFARFEKGQGLGAAPESVTPRRVGPLRAVDLVLKRPTDVIYQRVVLLDADLL
                     LMSVEAPREHDASARELAATFFDSLQVDAR"
     gene            complement(1177..1638)
                     /locus_tag="VGO02_12670"
     CDS             complement(1177..1638)
                     /locus_tag="VGO02_12670"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEV7478578.1"
                     /translation="MDLQGTVENLHARYAHAIDDDRLEDWPAFFTENGKYRIATAENE
                     ARGLPLPVLSCDSRAMLRDRIQSLRHANIYEPQSYRHIISSVILEKLDEKRVKSVASF
                     LVIRIMQDGATMVFASGRYLDRIVLAPEPLYEERVVVCDSRRFDTLLAIPL"
     gene            complement(1642..2862)
                     /locus_tag="VGO02_12675"
     CDS             complement(1642..2862)
                     /locus_tag="VGO02_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEV7478579.1"
                     /translation="MRAWPAEGATRVPYWVYQDPEVYEREQSRIWRGKTWSYLCLEAE
                     LPEPQSFVTTFVGDMPVVVTRGADNVLHAFENRCAHRGALICMQARGKTERFACIYHN
                     WTYDHAGNLTNVAFRRGIAGKGGMPADAKPESQAPRKLRVATLNGLVFGTLSAEVPSL
                     EDYIGPTILPRLKRVLQKPLKVLGAYTQVLPSNWKMYMDNVKDSYHASLLHLFFTRFR
                     INRLTQAGGVFVSDDGAHHCAYTKMVEDRGDVYERDGMRSAGSENKFVLENPEILKQV
                     DEFGDAVSIQIVSLFPGFVLHQIRNSLAARQVVPRGIDRTELHWTLFGFEDDDAAMTR
                     TRLLQSNLVGPAGYISMEDGVATDFVRRGVLGAPERASMIEMGGSGVATSESRITETA
                     VRGLWQAWRAHMDL"
     gene            2884..3843
                     /locus_tag="VGO02_12680"
     CDS             2884..3843
                     /locus_tag="VGO02_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7478580.1"
                     /translation="MYLSVRLIAVFSLCAANAAAQEWPQRTVRIVVPFPAGGSADLVP
                     RIAAEKLAQQWGQPVIIDNRPGAAGNIGADAVFKSDPDGYTLLSAPPPPLVINRLLYP
                     SLSYDPTQFVPITVIAAIPNVLLVNDKVPASSVAELIAFAKANPGKLNYASQGSGSTS
                     HLTAELFKSMAGGLEITHVPYRGTAPALADLLAGQVDIMCDNLGVSLPHVKSGKLRAL
                     GVASRMRSPSLPDVPTLAETLPGFEAVAWFGVVAPPKTPPAIAEKVAAGVREALKNPE
                     VLRRLQELSADPMGLTPAETAAFMTQETERWGRVIRSARVKVD"
     gene            4065..4253
                     /locus_tag="VGO02_12685"
     CDS             4065..4253
                     /locus_tag="VGO02_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478581.1"
                     /translation="MNPKLRRDRNAFLIFLAAVPVALLIALPLAVTNGKIYLAAKAHQ
                     QQSGAGGTQPAAAESNEK"
     gene            4255..5019
                     /locus_tag="VGO02_12690"
     CDS             4255..5019
                     /locus_tag="VGO02_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478582.1"
                     /translation="MLLSLVKAATDESGDRRLEREAQWLRELAGVESLEGQVPRLLEE
                     GWRDGHRYLVLSLAQAAVQETRAFTADHARFLASLGKARFRVTDFEVSGVCRWLQRAI
                     QEFQKEASFEELQRAYHDCEAALLYWTGPYVLSQGDFAPWNIRTFGAQLFVYDWGQAR
                     AEASPLDDVLHYLVVQRALRRRPVGVRFLRAAMRRAHDFARQAYPEWTWRPHVIGALT
                     LVYLLGAVLHHARQTRSCESSAYWRLLESRATWMPT"
BASE COUNT          887 a         1706 c         1780 g          874 t
ORIGIN      
        1 tcgagaacac cacgatctgc aggatctcgt tgttcgccat cgcctcggcg aaggacttcg
       61 gcaccaggtg gctgacgaag tccttgagcg tgaacttggc ggtggcgagg ttggccgagc
      121 tgccgatatc cggcagcggc aggccgaggt tgtggcccgg ctgcagcagg ttggcgagga
      181 tcaggccgag caccagcgac accagggagg cggtgatgaa ccagcccagc gacttggcga
      241 acacgcggcc gaccgaggcg gcgtcaccca tgtgcgcgat gccgaccacc agcgtcgaga
      301 agaccagcgg cccgatcagc atcttgatca gccgcaggaa cacgtccgcc aggatcgaga
      361 cgtagccggc gatctgcgcc gcagacttct tgtccgggaa ggacacgaag atcatgtacc
      421 cgacgatgat gccgagcacg agggcgacca gtatccagac cgccgctggt agtttcttct
      481 tcatcgctgc cctccctggg tggcgttcct gcccggctat cctactacct ggcgtcgact
      541 tgcagcgaat cgaagaaggt ggcggcaagc tcccgggcgc tcgcgtcgtg ctcgcgcggc
      601 gcttcgacgc tcatcaggag cagatcggcg tcgagcagga ccacccgctg gtagatcacg
      661 tcggtcgggc gcttgagcac caggtccacg gcgcgcagcg ggccgacacg gcgcggcgtg
      721 acgctctccg gcgccgcgcc gagcccctgg cccttctcga agcgcgcgaa atattccttg
      781 gccgccgccg cccctttgcg gtacgcgatg cgcgtcgcca cgaccgcgat gcgctcgctc
      841 gacctggcgc cgactgtgta ggtgcgcgcc gccggcgcgt tcgggtcggc ctcggtgacc
      901 gtgaagtcgt tgaacttgag cggcaggcgc acagagaagc ggccctcggt cgacttggcg
      961 agcatccagc ccgacttgtc gggctcgccc gcctgcttgc ggtgcatctt catctcttcc
     1021 gcgcccgccg tgccggccat caggcaaagc gaaaacagca gggcgatcca tttcaccagc
     1081 cagaaagcag cagacataag gcaccgccga acatgagtag ccccctgagc cccctggtgc
     1141 gccagaaata gtacgggaga tagggcggcc agaccactag agcgggatgg cgagcagggt
     1201 atcgaagcgc cgggagtcgc agacgacgac gcgctcttcg tagagcggct cgggggcgag
     1261 gacgatgcgg tcgaggtagc ggccgctggc gaagaccatg gtggcgccgt cctgcatgat
     1321 gcggatgacg aggaagctgg ccaccgactt cactcttttt tcatccagtt tttccaaaat
     1381 aacggaagaa atgatgtggc ggtagctctg cggctcgtag atgttggcgt ggcgcaggga
     1441 ctggatgcgg tcgcgcagca tggcgcgcga gtcgcaggac aggacgggga gggggaggcc
     1501 gcgcgcttcg ttctcggcgg tggcgatgcg gtacttgccg ttttcggtga agaacgccgg
     1561 ccagtcttcc aggcggtcgt cgtcgatggc gtgggcgtag cgggcgtgga ggttctcgac
     1621 ggtcccctgg agatccatct cttatagatc catgtgcgcg cgccaggctt gccacaggcc
     1681 gcgcacggct gtttcggtga tccgcgattc gctggttgcg acgccgctgc cgcccatctc
     1741 gatcatggag gcgcgctcgg gggcgcccag gacgccgcgg cgcacgaagt cggtggcgac
     1801 gccgtcctcc atcgagatgt agcccgcggg gccgaccagg ttggactgca gcagtcgggt
     1861 gcgggtcatg gccgcgtcgt cgtcttcgaa gccgaacagg gtccagtgca gctcggtgcg
     1921 gtcgatgccg cgcggcacga cctggcgcgc ggcgagggag ttgcggatct ggtgcaggac
     1981 gaagccggga aacagcgaca cgatctggat cgacaccgcg tcgccgaact cgtcgacttg
     2041 cttgagaatt tcagggtttt ccaatacaaa cttattttca gagccggctg agcgcatgcc
     2101 gtcgcgttcg tagacgtcgc cgcggtcctc gaccatcttc gtgtacgcgc agtggtgcgc
     2161 gccgtcgtcg gagacgaaca cgccgccggc ctgcgtcagg cggttgatgc gaaagcgggt
     2221 gaagaacagg tggagaaggc tggcgtggta cgagtccttc acgttgtcca tgtacatctt
     2281 ccagttgctc ggcagcacct gcgtataagc gccgagcacc ttcagcggct tttgcagcac
     2341 gcgcttcagg cgcggcagga tggtggggcc gatgtaatcc tcgagcgagg gcacctcggc
     2401 agagagcgtg ccgaacacca ggccgttcag cgtcgcaacc ctcagcttgc gcggcgcctg
     2461 gctctcgggc ttcgcatccg ccggcatgcc gcccttgccg gcaatgccgc gccggaaggc
     2521 gacgttggtc aggttgccgg cgtggtcgta ggtccagttg tggtagatgc aggcgaagcg
     2581 ctcggtcttg ccgcgcgcct gcatgcagat cagcgcgccg cgatgcgcgc agcgattctc
     2641 gaaggcgtgc aggacattgt cggcgccgcg cgtcaccacg accggcatgt cgcctacgaa
     2701 cgtggtgacg aagctctgcg gctcgggcag ctccgcctcg aggcacaggt agctccacgt
     2761 cttgccgcgc cagatgcgag attgttcccg ttcgtacact tcgggatcct ggtagaccca
     2821 gtagggaacg cgcgtcgcgc cttctgccgg ccatgcacgc atgcggctga ttataatgag
     2881 ccggtgtatc tctctgtccg gttaattgca gttttttccc tctgcgccgc gaacgcagcg
     2941 gcgcaggaat ggccgcagag gacggtgcgc atcgtggtgc cgtttcccgc cggcggcagc
     3001 gcagacctgg tgccgcgcat cgcggccgag aagcttgcgc agcaatgggg ccagccggtg
     3061 atcatcgaca accgccccgg cgcggccggc aacatcggcg ccgatgccgt gttcaagtcc
     3121 gacccggacg gctacacgct gctctcggcg ccgccgccgc cgctggtgat caaccggctg
     3181 ctgtatccga gcctgtccta cgatccgacg caattcgtgc cgatcacggt gatcgccgcg
     3241 attccgaacg tgctgctggt gaacgacaag gtgccggcga gctcggtggc cgagctgatc
     3301 gctttcgcaa aggccaatcc cggcaagctg aattacgcct cgcaaggcag cggcagcacc
     3361 tcgcacctca ccgcggagct cttcaagtcg atggccggcg ggctggagat cacgcacgtg
     3421 ccgtatcgcg gcaccgcgcc ggcgctggcc gacctcctcg ccggccaggt ggacatcatg
     3481 tgcgataacc tcggcgtctc gctgccgcac gtaaagtctg ggaagctgcg cgcgctcggg
     3541 gtggcgagcc gcatgcgctc gccgtcgctg cccgacgtgc cgacgctggc cgaaaccctg
     3601 cccggtttcg aagcggtggc ctggttcggc gtcgtcgcgc cgccgaagac cccgcccgcc
     3661 atcgccgaga aggtcgccgc gggcgtgcgc gaagccctga agaatcccga ggtgctcagg
     3721 cggctgcagg agctttccgc cgatccgatg ggcctcaccc cggcggaaac cgcggcgttc
     3781 atgacgcagg agaccgagcg ctggggccgc gtgatccgct cggcgcgggt aaaagtggat
     3841 tgatcgggga agcaattacc cgtttcgtcg gtcggtgacg tcgcgcccgc gcgacccgga
     3901 agctacagtt gattcaacag gttaggtggc cataggccgc ttgggggtcc cgccgcggcg
     3961 acgccgcagc acgccgtgca ttttctaaat cgttgattca gtgtcggttt acgtcgtggt
     4021 acgcgccttg cgagagcacc acaccaactc cgacaaggct ttccatgaat cccaagctgc
     4081 gccgcgacag gaacgcgttc ctgatcttcc tcgcggcggt acccgttgcg ttgctcatcg
     4141 cgcttccgct cgcggtgacc aacgggaaga tctacctggc tgcaaaggcc caccagcagc
     4201 agtcaggcgc gggcgggaca caacccgctg ccgccgaaag caacgagaag tagcttgctc
     4261 ctttccctgg tgaaggcggc gaccgacgag agcggcgacc gccgcctcga gcgcgaagcg
     4321 caatggctgc gcgagctcgc cggcgtcgag agcctcgagg gccaggtgcc gcgcctgctc
     4381 gaagagggct ggcgcgacgg ccaccgctac ctggtgctca gcctggcgca ggcggcggtg
     4441 caggagaccc gcgccttcac cgccgatcac gcgcgcttcc tcgccagcct gggcaaggcg
     4501 cgcttccgcg tcacggattt cgaagtgtcg ggcgtctgcc gctggctgca gcgcgccatt
     4561 caggaatttc agaaagaggc ctccttcgag gagctgcaac gcgcgtatca cgactgcgag
     4621 gcggccctgt tgtactggac cggcccgtac gtcctctcgc agggggattt cgcgccctgg
     4681 aacatccgca ccttcggcgc gcagctgttc gtctacgact ggggccaggc gcgcgccgag
     4741 gcgagcccgc tcgacgacgt gctgcattac ctggtggtgc agcgcgcgct gcggcgcagg
     4801 ccggtcggcg tgcgcttcct gcgcgccgcc atgcggcgcg cgcacgactt cgcgcgccag
     4861 gcctatcccg aatggacctg gcggccgcac gtcatcggcg cgctgacgct cgtctacctt
     4921 ctcggcgcgg tgctgcatca cgcgcggcag actcgttctt gcgagagcag cgcgtactgg
     4981 aggctgctcg agtcgcgcgc aacctggatg ccgacatgaa aaacgagaac ctacggaaaa
     5041 gaaacgtttg aaaaatctta tgaggacact atgagcgctg cagccaccgc cgccggatat
     5101 ctctatgcct cgggtcgcct gcgcggccgc aacggccgcg acaacgagca ccacgacaac
     5161 gaccatcacc gcgaccatga gcggcactac ggccgcggcc aggacgagcc ggcgcccgag
     5221 gtgaagaagc cgcagctgaa agtggcc
//
LOCUS       DASYJA010000578         3606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_3084029, whole genome shotgun sequence.
ACCESSION   DASYJA010000578 DASYJA010000000
VERSION     DASYJA010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3084029"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /gene="ptsP"
                     /locus_tag="VGO02_12695"
     CDS             complement(<1..1430)
                     /gene="ptsP"
                     /locus_tag="VGO02_12695"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEV7478583.1"
                     /translation="MNFVLHGHSVSTGITIGQAHLVSSARLEVAHYEIEDDKVDAEIA
                     RFDAALRAGREELSELKAHIPADAPAEVEAFLNLHRMILDDSALSQVPRELIRTRHCN
                     AEWALVQQMEKLTDRFDEIEDPYLRERKADVQQAVERVLKEMMGGSRPPEPALSDEKE
                     LIVVAHDLSPADMILFKKHRFGGFVTDMGGVTSHTAIVARSLGIPAIVGLHSARHMVS
                     EGETLIVDGQNGVLIVNPDPLVLAEYRVRESQLGLERQRLRRLKSTPAATIDGTPIEL
                     YANIELPQDMAQVLDAGAQGVGLFRTEFLFLNRQGLPDEDEQFTAYREVAEAMQGRPV
                     VLRTLDIGADKALNGNGQGAGSMPNPAMGLRAIRFCLAEPQMFLTQLRAILRASHYGK
                     VRILLPMLAHVHEIEQTLTLIRQAKQQLDEKSLPYDREVQIGGMIEIPAAALALPIFM
                     KRLDFLSIGTNDLIQYTLAIDRTDDAV"
     gene            complement(1439..1708)
                     /locus_tag="VGO02_12700"
     CDS             complement(1439..1708)
                     /locus_tag="VGO02_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEV7478584.1"
                     /translation="MPSADAEIINKLGLHARASAKLTQLAGGFESDIWISRSGRRVNA
                     KSIMGVMMLAAGIGATVKLEAEGKDAERALAALLQLIRDKFGEGE"
     gene            complement(1701..2105)
                     /locus_tag="VGO02_12705"
     CDS             complement(1701..2105)
                     /locus_tag="VGO02_12705"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV7478585.1"
                     /translation="MIGVLIVTHGSIGESLLSSASQILGGLPPLVATLGVSRNDDPDD
                     IRLRAGELVEGLDQGDGVLVLTDIFGATPGNAVSHLLDDGHIEGVSGISLPMLLRVLT
                     GRNGSLPGAVQRALSGGAEGLVHMNNDRCLDA"
     gene            complement(2102..3049)
                     /gene="gshB"
                     /locus_tag="VGO02_12710"
     CDS             complement(2102..3049)
                     /gene="gshB"
                     /locus_tag="VGO02_12710"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEV7478586.1"
                     /translation="MAARILFVVDPPDSLKAYKDTTVTMMRAAQKRGHAVWVCEQEGL
                     HWRNNLVQTHATRISLTDNDEDWYRPHETVVEPLKSFGAVLMRKDPPFDMEYVSSTWL
                     LSEAERQGAKVFNAPRALRDHNEKFAIAEFSRFTVPSRVARAMGELQAFVDEHKTTVL
                     KRLDGMGGTGIFVVRHDDPNRNSIVETISALGTRSVMAQRYIPEIKDGDKRVLIVDGK
                     LVPHCLARIPKPGESRGNLAAGARGVAQPVTSRHREIAAELGPVLAKRGLLLVGLDVI
                     GDWLTEVNVTSPTCFREIQDQTGFDVAGVFLDALESKLA"
     gene            complement(3051..>3606)
                     /locus_tag="VGO02_12715"
     CDS             complement(3051..>3606)
                     /locus_tag="VGO02_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEV7478587.1"
                     /translation="KSYLYLIPGPTAVSTHDEHSSFIKTPQQLITVILAAFLVPIIGI
                     ILLVQLVTGQPGADPAAMTPEKIAERIKPVSGIEMGAPAAAPGARGGEAIVKATCAAC
                     HQAGVAKAPKIGDKNDWAPHLKQGQSALVQSAIKGKGAMPPRGGDGSLTDAEVARAVV
                     FMANSAGAGFKESAAPGAAKKKSK"
BASE COUNT          517 a         1223 c         1242 g          624 t
ORIGIN      
        1 accgcgtcgt cggtgcggtc gatggcgagg gtgtactgga tcaggtcgtt ggtgccgatc
       61 gacaggaagt ccaggcgctt catgaaaatc ggcagcgcga gcgccgccgc cgggatctcg
      121 atcatgccgc cgatctgcac ctcgcggtcg tagggcaggc tcttctcgtc gagctgctgc
      181 ttcgcctggc ggatcagggt gagggtctgc tcgatctcgt gcacgtgggc gagcattggc
      241 aggaggatgc gcaccttgcc gtagtgcgag gcgcgcagga tggcgcgcag ctgcgtgagg
      301 aacatctgcg gctcggcgag gcagaagcgg atcgcgcgca gccccatcgc cgggttgggc
      361 atgctgcccg cgccctggcc gttgccgttc agcgccttgt cggcgccgat atcgagggtg
      421 cgcagcacca ccggccggcc ctgcatcgcc tcggcgacct cgcggtaggc ggtgaactgc
      481 tcgtcctcgt cgggcaggcc ctggcggttg aggaacagga actcggtgcg gaacaggccc
      541 acgccctgcg cgccggcgtc gagcacctgc gccatgtcct gcggcagctc gatgttggcg
      601 tacagctcga tcggcgtgcc gtcgatggtc gccgccggcg tcgacttcag gcggcgcagc
      661 cgctggcgct ccaggccgag ctgcgactcg cgcacccggt actcggcgag caccagggga
      721 tcggggttga cgatcagcac gccgttctgg ccgtcgacga tcagcgtctc gccctcgctg
      781 accatgtggc gcgcggaatg caggccgacg atcgccggga tgccgaggct gcgcgccacg
      841 atcgcggtgt gcgaggtgac cccgcccatg tcggtgacga agccgccgaa gcggtgcttc
      901 ttgaacagga tcatgtcggc gggcgacagg tcgtgcgcca ccacgatcag ctccttttcg
      961 tccgacagcg ccggctcggg cggccggctg ccgcccatca tctccttcag cacgcgctcg
     1021 accgcctgct gcacgtcggc cttgcgctcg cgcaggtagg ggtcctcgat ctcgtcgaag
     1081 cggtcggtga gcttttccat ctgctgcacc agcgcccact cggcgttgca gtgacgggtg
     1141 cggatcagct cgcgcggcac ctgcgacagc gccgagtcgt cgaggatcat gcggtgcagg
     1201 ttgaggaagg cctcgacctc ggccggcgcg tcggccggga tgtgggcctt cagctcggag
     1261 agctcctcgc gccccgcgcg cagggcggcg tcgaagcgcg cgatctcggc atcgaccttg
     1321 tcgtcctcga tctcgtagtg cgccacctcg agccgcgcgc tcgagacgag gtgcgcctgg
     1381 ccgatggtga tgccggtgga gacggaatgg ccgtgcagga cgaagttcat gccgagcgct
     1441 attcgccttc gccgaacttg tcccggatca gctgcagcag cgcggcgagc gcccgctcgg
     1501 catccttgcc ctcggcctcg agcttcaccg tggcgccgat gccggcggcg agcatcatca
     1561 cgcccatgat gctcttcgcg ttgacgcgcc ggccgctgcg cgagatccag atgtcggact
     1621 cgaagccgcc ggccagctgg gtgagcttcg cggaggcgcg cgcatgcaag cccagcttgt
     1681 tgatgatctc ggcgtcagcg ctaggcatcc aggcaccgat cgttgttcat gtggaccagg
     1741 ccctcggcgc cgcccgagag cgcgcgctgc accgcccccg gcagcgagcc gttgcgcccg
     1801 gtgagcacgc gcagcagcat cggcagcgag atgccggaga cgccctcgat atggccgtcg
     1861 tcgagcaggt gcgagacggc gttgcccggc gtggcgccga agatgtcggt cagcaccagc
     1921 acgccgtcgc cctgatcgag cccttccacc agctcgccgg cgcgcagcct gatgtcgtcg
     1981 gggtcgtcgt tgcgcgagac gccgagcgtg gcgacgagcg gcggcaggcc gccgagtatc
     2041 tggctggcgc tcgacagcag cgactcgccg atcgagccgt gcgtgacgat gagcacgccg
     2101 atcatgcgag cttgctctcc agcgcatcga ggaacacgcc cgctacgtcg aagccggtct
     2161 ggtcctggat ctcgcggaag caggtcgggc tggtgacgtt gacctcggtg agccagtcgc
     2221 cgatcacgtc caggccgacc aggagcaggc ctcttttcgc gagcaccggt cccagctccg
     2281 ccgcgatctc ccgatgccgc gacgtgaccg gctgcgccac gccgcgcgcg ccggcggcga
     2341 ggttaccgcg cgactcgccc ggcttgggga tgcgcgccag gcagtgcggc accagcttgc
     2401 cgtcgacgat cagcacgcgc ttgtcgccgt ccttgatctc ggggatgtag cgctgcgcca
     2461 tcacgctgcg cgtgccgagc gcggaaatgg tctcgacgat cgaattgcgg ttcgggtcgt
     2521 cgtggcgcac gacgaaaatc ccggttcccc ccatcccgtc cagcctcttc agtacggtgg
     2581 tcttgtgttc atccacgaat gcctgcagct cgcccatcgc gcgcgcgaca cgggagggca
     2641 ccgtgaacct ggagaactcg gcgatcgcga acttctcgtt gtgatcgcgc agcgcgcgcg
     2701 gcgcgttgaa aaccttcgcg ccctgccgct cggcttcgga aagaagccac gtgctcgaca
     2761 cgtactccat gtcgaaaggc gggtccttgc gcatcagcac cgcgccgaag gacttgagcg
     2821 gctcgaccac cgtctcgtgc gggcggtacc agtcttcgtc gttgtcggtg agggagatgc
     2881 gggttgcatg ggtttgaaca agattgttcc tccaatgcag gccctcttgc tcacaaaccc
     2941 agaccgcgtg gccgcgcttc tgcgcggcac gcatcatcgt gaccgtcgtg tccttgtacg
     3001 ccttgagcga atcaggcgga tcgacgacga acaggatccg cgcggccagg ctacttgctc
     3061 tttttcttcg cggctcccgg cgcagcagat tccttgaagc ccgcgccggc ggagttcgcc
     3121 atgaatacca ctgcgcgcgc gacctcggcg tcggtgagcg agccgtcgcc gccgcgcggc
     3181 ggcatcgcgc ccttgccctt gatcgcgctt tgcaccagcg cgctctggcc ctgcttgagg
     3241 tgcggcgccc agtcgttctt gtcgccgatc ttcggcgctt tggcgacgcc tgcctggtgg
     3301 caggccgcgc aggtcgcctt gacgatggct tcgccgccgc gcgcgccggg cgcggccgcg
     3361 ggcgcgccca tctcgatgcc cgacaccggc ttgatgcgct cggcgatctt ctccggcgtc
     3421 atcgccgccg ggtcggcgcc gggctgcccg gtgaccagct gcaccagcag gatgatgccg
     3481 atgatcggca cgagaaacgc ggcgaggatc accgtgatca actgctgcgg cgtcttgatg
     3541 aaggacgaat gctcgtcgtg cgtgctcact gccgttggcc ccggtatgag ataaaggtat
     3601 gatttt
//
LOCUS       DASYJA010000579         3663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1811766, whole genome shotgun sequence.
ACCESSION   DASYJA010000579 DASYJA010000000
VERSION     DASYJA010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811766"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VGO02_12720"
     CDS             complement(<1..369)
                     /locus_tag="VGO02_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEV7478588.1"
                     /translation="MRREIRFTLNGAAVSAQVAPHESLVEVLQEAFGLYGARESCGQG
                     LCGCCTVLVNGTAVSGCLYLGTLAENAEIQTIEAGEQLDAVQEAFIESGAFQCGFCTP
                     GFILMTRQLLDEIPQPDEEQI"
     gene            complement(366..2636)
                     /locus_tag="VGO02_12725"
     CDS             complement(366..2636)
                     /locus_tag="VGO02_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEV7478589.1"
                     /translation="MKRATQPTETAQVGRAVPRLESWLKVTGRAEYVHNLRLPGMLYG
                     KIFRSTVSHGRIKRIDVSAAQAVAGVYRVITSREIRELVPDPYYGPAFHDQPILAVDK
                     VRHVGEPVAVVLASDPHVAEAAAHLIVAEYEPLPAVYDEVEAMSSKAIVHDQLKPAGT
                     FPDLKHLKGKANTNIALDFHLRRGDAPGALKSAHKVFEHRFRTQQVMHTPLEPLVSVA
                     ECTDRTLTLHTASQSPSFVRIEIARLLGWPENRVRVRVPFLGGGFGAKLYIKLEALVS
                     VLSLLTRRPVKISLTIKEQFFTITKHATTFRIKSGVDRGGRIVARECEVWWNGGAYAD
                     IGPRVTQKSGFTAAGPYDIENVAVDSYALYTNLPPAGALRGFGIPQLVWAYESHTDMM
                     ARELGIDPVDFRRKNLLREGRPQATGTILRDAATDKVLERLSILMDWEKPFERGSGIV
                     RRGRGIAIGFKACVSPTTSLAAVSVNADGSCTVYCSTVDMGQGSDTAMAQIAAEVLNV
                     ATESVRVVHSDTDVTPYDMATLGSRSLFHMGNAVKLAAQDALKKLASLKEETQLPPDA
                     AIKDVFKKKYGMQAGNVLGTASFVPAYTPPDANGQTENATPFWMVGASGVELEVDTET
                     GRVRVTRLVNVADVGTPINPRIVETQLSGAAIMQLGFTLFEKMQFDAEGQLRNASLAE
                     YRIPGFLDVPPSIVNEAVAAETRSGPFGAKGVGETSTFGVSPAIANAVHDAVGVRITS
                     LPLTAETIFMKLQENR"
     gene            complement(2611..3390)
                     /locus_tag="VGO02_12730"
     CDS             complement(2611..3390)
                     /locus_tag="VGO02_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEV7478590.1"
                     /translation="MQPFELAVPGSLREAIALLDTDDARPFSGGTALMLMMKAGVLRP
                     SRLVSLGRLGLDRIELGPAGELRLGAMVSLRKIETSEVVKRGWPSITRTLLTLSNVRV
                     RNVARLGGHLAHGDPHTDLPPLLSALGAVATIAGPGGERHLPVESLYAGYLETTLARN
                     ELIVRVEVPAMGKKQAAYLKCTTRSIDDWPAVGVAVVLDGDQQTIVVSAATDKPTRLA
                     SADAVEQLEIEGDLHGSAAYKKHLIRVYVRRAIHEARNAAH"
     gene            complement(3458..>3663)
                     /locus_tag="VGO02_12735"
     CDS             complement(3458..>3663)
                     /locus_tag="VGO02_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV7478591.1"
                     /translation="TALHPQTLLTFRFADQILPPMYGFPMKLRIPTKLGFKNPKHIAA
                     IFVTNTYPGGYWEDQGYNWYSGS"
BASE COUNT          584 a         1201 c         1216 g          662 t
ORIGIN      
        1 gatctgctcc tcgtccggct gcgggatctc gtcgagcagc tggcgcgtca tgaggatgaa
       61 tcccggcgtg cagaagccgc actggaaggc gccgctttcg atgaacgcct cctgcacggc
      121 gtcgagctgc tcgcccgctt cgatggtttg gatttcggcg ttttcggcaa gcgtgccgag
      181 gtaaaggcat cccgataccg cggtgccgtt gaccagcacc gtgcagcagc cgcacaggcc
      241 ctggccgcag ctttcgcgcg cgccgtacag gccgaacgcc tcctgcagca cttcgaccag
      301 gctctcgtgc ggcgcaacct gcgccgagac cgccgcgccg ttcagcgtga agcggatttc
      361 gcgcctcaac ggttttcctg aagtttcatg aaaatggttt cagcagtaag cggcaaggac
      421 gtaatgcgta cgcccaccgc atcgtgcacg gcgttggcga tcgccggcga gacgccgaag
      481 gtcgaggttt cgcccacccc cttggcgccg aacggaccgc tgcgcgtctc cgccgcgact
      541 gcctcgttga cgatgctcgg cggcacgtcg aggaaacccg ggatcctgta ctcggcgagc
      601 gaggcgttgc gcagctggcc ctcggcgtcg aactgcatct tctcgaaaag cgtgaagccg
      661 agctgcatga tcgcggcgcc ggaaagctgc gtctcgacga tccgcgggtt gatcggcgtg
      721 cctacgtcgg cgacgttgac caggcgcgtc acccgcaccc ggccggtctc ggtgtcgacc
      781 tcgagctcca cgccgctcgc cccgaccatc cagaacggcg tcgcgttttc ggtctgcccg
      841 ttggcgtcgg ggggcgtata ggccggcacg aaactcgccg tgccgagcac atttcccgcc
      901 tgcatgccgt attttttctt gaacacgtcc ttgatcgcgg catccggtgg aagttgcgtt
      961 tcctctttca gggatgccag tttcttgagg gcatcttgcg cggcgagctt gacggcgttg
     1021 cccatgtgga acagcgagcg cgagccgagc gtcgccatgt cgtagggcgt gacgtcggtg
     1081 tcggaatgca ccaccctgac cgactccgtg gccacattca gcacctcggc ggcgatctgc
     1141 gccatggcgg tatccgagcc ctgacccatg tcgacggtgc tgcagtagac ggtgcagctg
     1201 ccatcggcgt tcacgcttac cgcggcaagc gaggtggtcg gcgaaacgca ggccttgaag
     1261 ccgatggcga tgccgcggcc gcggcgcacg atgccgctgc ctcgctcgaa cggcttctcc
     1321 caatccatga gaattgaaag tctttccaaa accttgtcgg tcgccgcatc gcgcaagatg
     1381 gtcccggtcg cctgcggcct gccctcgcgc agcaggttct tcctgcggaa gtccaccggg
     1441 tcgatgccca gctcgcgcgc catcatgtcg gtgtggctct cgtaggccca caccagctgc
     1501 gggatgccga agccgcgcag cgcgccggcc ggcggcaggt tggtgtacag ggcgtacgaa
     1561 tcgaccgcga cattctctat gtcgtagggg ccggccgcgg taaagcccga tttctgcgtc
     1621 acgcgcggcc cgatgtcggc gtaggcgccg ccgttccacc acacctcgca ttcgcgcgcg
     1681 acgatgcggc cgccgcgatc cactccgctc ttgatccgga aggtcgtggc gtgtttcgtg
     1741 atggtgaaga actgctcctt tatcgtcagc gagattttta cgggtctcct ggtcaaaaga
     1801 gaaagaacgc tgaccagcgc ctcgagcttg atgtagagct tggcgccgaa gccgccgcca
     1861 agaaaaggca cacgcacccg cactctgttc tccggccagc cgagcagccg cgcgatctcg
     1921 atgcgcacga aggacgggct ctgcgacgcg gtatgcaggg tcagcgtgcg atcggtgcac
     1981 tcggccaccg acaccagcgg ctcgagcggc gtgtgcatca cctgctgcgt gcggaagcgg
     2041 tgctcgaaca ccttgtgcgc gctcttcagc gcacctggcg catcgccgcg ccgcagatgg
     2101 aagtcgagcg cgatattggt gttggccttg cctttcaggt gcttcaggtc cgggaaggta
     2161 cccgccggct tgagctggtc gtgcacgatc gccttcgagc tcatcgcctc gacttcgtcg
     2221 tacacggcgg gcagcggctc gtactcggcg acgatgaggt gcgccgccgc ttccgcgacg
     2281 tgcgggtcgg aggcgagcac caccgcgacc ggctcgccga catggcgcac cttgtcgacg
     2341 gcgaggatcg gctggtcgtg gaacgccggg ccgtagtagg gatcgggcac cagttcgcga
     2401 atctccctgg aagtaatcac ccgatacacc ccggcgacgg cctgcgcggc cgagacgtcg
     2461 atgcgcttga tgcggccgtg cgatacggtg ctgcggaaga tcttgccgta gagcatcccc
     2521 ggcaggcgca ggttgtggac gtactcggca cgaccggtga ccttgagcca cgactccagt
     2581 cgcggcacgg cgcggccgac ctgcgccgtc tcagtgggct gcgttgcgcg cttcatggat
     2641 cgcccgcctg acatagacgc ggatcaggtg cttcttgtag gcggccgatc cgtgcaggtc
     2701 gccttcgatc tcgagctgct ccaccgcgtc tgccgaggcc agcctggtcg gcttgtcggt
     2761 cgccgcgctt acgacgatcg tctgctggtc gccatccagc acgaccgcga ctccgaccgc
     2821 gggccagtcg tcgatcgagc gcgtggtgca tttgaggtag gcggcctgct ttttccccat
     2881 ggcgggcacc tcgacgcgca cgatcagctc attgcgcgcg agcgtggtct cgaggtagcc
     2941 ggcgtacagc gactcgaccg gcaggtggcg ctcgccgccg ggccccgcga tggttgccac
     3001 ggcgccaagc gcgctcagta gcggcggcag atcggtatgc ggatcgccgt gcgcgaggtg
     3061 gccgcccagc cgggcgacgt tgcgcacgcg cacgttcgac agcgtgagca gcgtgcgggt
     3121 aatagacggc cagccgcgct ttaccacttc gcttgtttca atttttctca gggaaaccat
     3181 ggcgccgagg cgcagctcgc cggccggccc gagttcgatc ctgtccaggc cgagccggcc
     3241 gaggctgaca agacgcgacg ggcgcagcac gcccgccttc atcatcagca tgagcgccgt
     3301 gccgccggag aacggccgcg cgtcgtcggt atcgagtagc gcgatcgcct cgcgcagcga
     3361 tcctggcacg gccagctcga aaggctgcat cactcctcct gcgcggcaat ctagcacagg
     3421 gcaaaacgcc ggacgcggcg agcgtcaggt catttcgcta cgaaccgcta taccagttgt
     3481 agccttggtc ttcccagtag ccgccgggat acgtgttggt cacgaagatg gccgcgatgt
     3541 gcttcggatt cttgaagccc agcttggtcg ggatgcgcag cttcatggga aagccgtaca
     3601 tcggggggag gatctggtcg gcaaagcgga aggtgagcag cgtctgcgga tgcagggccg
     3661 tgg
//
LOCUS       DASYJA010000580         1761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2265465, whole genome shotgun sequence.
ACCESSION   DASYJA010000580 DASYJA010000000
VERSION     DASYJA010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2265465"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /locus_tag="VGO02_12740"
     CDS             <1..1292
                     /locus_tag="VGO02_12740"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV7478592.1"
                     /translation="LLARALQYQRVEGGSLQGFLRWFEAGGGEIKRDLDQNRRREVRI
                     LTVHASKGLQAPIVYLPDTTRVPRDSERLLAGADGEARLWLPRADDANEAARSWRADA
                     RERALQEQNRLLYVAMTRAEDRLYVGGWVGAKKPDRGCWYERIEAGLRASSDDGVVRR
                     IGETRGRAVARAFDFTAQLGDEGWLGDGYELTNPGNIEIPEQAELPLELAARLEPWMH
                     QPAPDEPDPPTPLAPSQPLPDEALAGPRAFSPLAPEDSRRWQRGRLLHELLRHLPALP
                     VAARAPAARRFLAQPAHGLTEEEISLWADEALAVTGAPDHAALFAEGSRAEVPLIGTV
                     KTPRGTFTVSGQVDRLAVSPREVLIVDYKTNRPPPQSASGVALAYRRQLALYRSLLAD
                     IYPGRTVRAFLLWTAAPLLMEIDAETLEKSMPYAAAS"
     gene            1376..1696
                     /gene="trxA"
                     /locus_tag="VGO02_12745"
     CDS             1376..1696
                     /gene="trxA"
                     /locus_tag="VGO02_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEV7478593.1"
                     /translation="MTVKATDTSFEQEVLKSDTPVLVDFWAEWCGPCRMIGPSLEDIS
                     KEMDGKLKVVKVNIDENPTTPSRYGVRSIPTLLLFKNGQVAATKIGAEPKQKLVSWIN
                     TAIA"
BASE COUNT          293 a          595 c          599 g          274 t
ORIGIN      
        1 agctgctggc gcgcgcgctg cagtaccagc gtgtcgaggg cggatcgctg caagggttcc
       61 tgcgctggtt cgaggcggga ggcggcgaga tcaagcgcga tctcgaccag aaccgtcgcc
      121 gcgaagtgcg catcctgacg gtgcacgcgt cgaagggcct gcaggcgccg atcgtctacc
      181 tgcccgacac gacccgtgtg ccgcgcgata gcgaacggtt gctggccggc gccgacggcg
      241 aggcccggct gtggctgccg cgcgccgacg acgccaatga ggccgcgcgc agctggcgcg
      301 ccgatgcccg cgagcgcgcg ctgcaggagc agaaccgcct gctctatgtc gccatgaccc
      361 gcgccgagga tcggctctat gtcggcggct gggtgggggc gaagaagccg gaccgcggct
      421 gctggtacga gcgcatcgag gcgggcttgc gcgccagcag cgacgacggc gtggttcgca
      481 gaatcggcga gacccgcggt cgcgccgtgg ctcgcgcctt cgacttcacg gcgcagctcg
      541 gcgatgaagg ctggctgggc gacggctatg aactcaccaa ccccggcaat atcgagatac
      601 ccgaacaggc cgagttgccc ctcgagctgg cggccaggct ggagccctgg atgcaccaac
      661 cggcgccgga cgagcccgat ccaccgacac cgctggcgcc ctcgcagccg ctgcccgacg
      721 aggcgcttgc gggtccacgg gccttcagcc cgctggcgcc ggaagattcg cggcgctggc
      781 agcgcggccg gctgctgcac gagctgctgc gccatctgcc ggccctgccg gtggcggcgc
      841 gcgcaccggc ggcgcggcgt ttcctcgcgc aaccggcgca tggattgacc gaagaggaga
      901 tctcgctgtg ggccgacgag gccctcgccg tcaccggcgc gcccgaccat gccgctttgt
      961 tcgccgaagg ctcacgcgcc gaggtgccgc tgatcggcac tgtgaagacg ccgcgcggca
     1021 ccttcaccgt gagcggccag gtcgaccgcc tggccgtctc tccgcgcgag gtgctgatcg
     1081 tcgattacaa gaccaaccgg ccgccgcctc aatcggcttc gggcgtggcg ctggcctatc
     1141 gccgacagct cgcgctctat cgcagcctgc tcgccgacat ctatcccggg cgcaccgtac
     1201 gggcttttct gctgtggaca gcagcccctt tgttgatgga aatcgatgca gaaacgcttg
     1261 aaaaatcaat gccttacgct gccgcatctt gactcgcccg accgcggttc ctagcttggg
     1321 gtccaagggc ggcgccggtc catcgaccgg cctgtagtgt ttttggagaa cctctatgac
     1381 cgtcaaagca acagatacct ccttcgagca ggaagtcctg aagtccgaca cgcctgtgct
     1441 cgtcgatttc tgggcggagt ggtgtgggcc gtgccgaatg atcggcccct cgctggaaga
     1501 catctccaag gagatggacg gcaagctgaa ggtggtgaag gtcaacattg acgagaatcc
     1561 gacgacgccg tcgcgttacg gtgtccgctc gatccccacg ctgctgctgt tcaagaacgg
     1621 ccaggtcgcc gcgaccaaga tcggcgccga gcccaagcag aagctggtga gctggatcaa
     1681 caccgcgatc gcttaaacga ttaacctcat cctgaggagc cgcgcgcagc gcggcgtctc
     1741 gaaggatggc acgagcaccg c
//
LOCUS       DASYJA010000581         2279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2721061, whole genome shotgun sequence.
ACCESSION   DASYJA010000581 DASYJA010000000
VERSION     DASYJA010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2721061"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VGO02_12750"
     CDS             <1..613
                     /locus_tag="VGO02_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478594.1"
                     /translation="AMRDDKATLGWPFSLALNHLLDAQPEAKQRLAPFAGQSVELRAP
                     PLPALTFTILPGGRVEGEMQTRGGAEPALVVTLKRGFPAALAKGEDYLMREVEVSGEP
                     RLAAEVMWLVRNLREALADIAEEDLSRVVGDVVAHRLARGARDFLTWQADAAGRLAAA
                     FADYATEESRILVARAEHESFAAAVSRLRDALERLEKRIGHLG"
     gene            606..2099
                     /gene="ubiB"
                     /locus_tag="VGO02_12755"
     CDS             606..2099
                     /gene="ubiB"
                     /locus_tag="VGO02_12755"
                     /inference="COORDINATES: protein motif:HMM:NF003404.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEV7478595.1"
                     /translation="MARLLRVARIFAVGLRFGLYEFIPRFSYNRLFISKTKPRGQRLR
                     EALETLGPIFVKFGQVLSTRRDLLPLDIADELAKLQDQVPPFPSELAVLEIERSLGKP
                     IHELFDSFEQLPVASASIAQVHLAVLHDGREVAVKVLRPRVEAAIARDLALMETAAGL
                     VERLWIDGRRLKPRQVVAEFAHHLDEELDLMREAANASQLRRNFKGSALLVVPEMHWD
                     LCSRRVMVMERMHGTPISQVEKLREKGIDIPALARAGVEVCFTQVFRDGFFHADMHPG
                     NILVADDGKYVALDFGIMGTLTEADKNYLARNFIAFFNRDYRRVAQAHLDAGWVPAGT
                     RVDEFESAIRAVCEPIFARPLKEIYFGRLLLRLFQTARRFNMEVQPQLVLLQKTLLNI
                     EGLGRELDPDLDLWRTAKPYLERWMNEQIGWRGLLRAVRQEAPFWAAALPQLPRLVHR
                     ALSEDRLGGLHDTLKRLADESARRNDLLSATLVILTAALIVAAVALL"
BASE COUNT          353 a          757 c          798 g          371 t
ORIGIN      
        1 cgccatgcgc gacgacaagg ccaccctcgg gtggcctttt tctttggcgt tgaaccacct
       61 gctcgacgcg cagccggagg cgaagcagcg gctcgcgccc ttcgccggac agagcgtcga
      121 attgcgcgcc ccaccgctgc ccgcgctaac cttcacgatt ctgccgggcg gccgggtgga
      181 aggggaaatg cagacgcgcg gcggcgccga gccggcgctc gtcgtcacgc tcaagcgcgg
      241 ctttcccgcc gcgctggcga agggggagga ctatctgatg cgcgaggtcg aggtgagcgg
      301 cgagccgcgc cttgcagccg aagtgatgtg gctggtgcga aacctgcgag aagcgcttgc
      361 cgatatcgcc gaggaggatc tgtcgcgcgt ggtcggcgac gtcgtcgcgc atcgcctcgc
      421 gcggggcgcg cgcgatttcc tcacctggca ggccgacgcg gccgggcggc tcgccgcggc
      481 gttcgcggac tatgccaccg aggagagccg catcctcgtc gcgcgcgcgg agcatgaaag
      541 cttcgcagcc gcggtgtcgc ggctgcgcga cgcgctcgag cggctggaaa agcggatcgg
      601 gcaccttggc taggctcctg cgcgtcgcgc gcatcttcgc ggtcggcctg cgcttcgggc
      661 tgtacgagtt cattccgagg ttttcctaca acaggctttt cataagcaag acaaaaccgc
      721 gcgggcaaag actgcgcgaa gcccttgaaa ccctcggccc gatcttcgtc aagttcgggc
      781 aggtcctgtc gacgcggcgc gacctgctgc cgctcgacat cgccgacgag ctggcgaagc
      841 tgcaggacca ggtgccgccg tttccttcgg agctggcggt cctggagatc gagcgctcgc
      901 tgggcaagcc gatccacgaa ttgttcgatt cattcgaaca actgcccgta gcgagcgcgt
      961 ccattgcgca ggttcacctg gcggtgctgc acgacgggcg cgaggtggcg gtcaaggtgc
     1021 tgcgcccgcg cgtcgaggcg gcgattgcgc gcgacctggc gctgatggag accgccgccg
     1081 gactcgtcga gcggctgtgg atcgacggcc ggcgcctcaa gccgcgccag gtggtggccg
     1141 agttcgcgca ccacctggac gaggaactcg acctgatgcg cgaggcggcg aacgccagcc
     1201 agctgcggcg caacttcaag ggatcggcgc tgctggtggt tcccgagatg cactgggacc
     1261 tttgctcacg gcgcgtgatg gtcatggagc gcatgcacgg cacgccgatc tcccaggtgg
     1321 aaaaactgcg ggagaagggc atcgacatcc cggccctcgc ccgggcgggc gtcgaggtct
     1381 gcttcacgca ggtgttccgc gacggtttct tccacgcaga catgcacccg ggcaacatcc
     1441 tggtggccga cgacggcaag tacgtggcgc tcgacttcgg catcatgggc acgctcaccg
     1501 aggccgacaa gaactacctg gcgcgcaatt tcatcgcctt cttcaaccgc gattaccgcc
     1561 gcgttgcgca ggcgcacctc gacgccggct gggtgccggc gggcacgcgt gtcgacgagt
     1621 tcgaaagcgc catccgcgcc gtgtgcgagc cgatcttcgc gcggccgctg aaggagatct
     1681 atttcgggcg gctgctgctg cgcctgttcc agaccgcgcg gcgcttcaac atggaagtgc
     1741 agccgcagct ggtgctgctg cagaagacgc tgctcaacat cgagggcctg ggccgcgagc
     1801 tcgatccgga cctcgacctg tggcgcaccg ccaagccgta tctggagcgc tggatgaacg
     1861 agcagatcgg ctggcgcggc ctcctgcgcg cggtccgcca ggaggcgccg ttctgggcgg
     1921 cggcgctgcc ccagctgccg cgcctggtgc atcgcgcgct cagcgaggac cgcctcggcg
     1981 ggttgcacga tacgctgaag cgcctggccg acgagagcgc gcggcgcaac gacctgctgt
     2041 cggccaccct ggtgatcctc actgcggccc tgatcgtcgc cgccgtcgcc ctgctgtagt
     2101 gccgcaggac agctcgcgcg cggatttctg ggacacgcgc tttcgcgacg gcgtcacgcc
     2161 gtgggacgcc ggaggggttc ctgctcagct ggcaatgtgg ctggggagga agacatcccc
     2221 tatgagaatc ctcgttccag gatgcggcag cggctacgaa gtgcgcctgt ttaaccacc
//
LOCUS       DASYJA010000582         2071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1267903, whole genome shotgun sequence.
ACCESSION   DASYJA010000582 DASYJA010000000
VERSION     DASYJA010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267903"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1099)
                     /locus_tag="VGO02_12760"
     CDS             complement(104..1099)
                     /locus_tag="VGO02_12760"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEV7478596.1"
                     /translation="MKALFLIAGILLCHVAGAADRLAGEHADVKTFSVSGLSSGGFMA
                     IQFQVAHSGRVKGVAALAAGPYYCAQGSLWAAYYNCTTPGSWTPLPSIEALKAQAEHF
                     ASEGRIDSTGNLAATRLWFFSGSRDRTVARPVVEAAARFYALFGAKNVPIVARPAGHA
                     MVTEDAGTACGTTEAPFINDCDYDAAGALLAQLLGPLKPASKEGGRLVAFDQKSFADG
                     NPYAISMADSGYVYVPTACLSARCAVHVVFHGCRQNADAVGDQFVRQAGYNRWADTNR
                     LIVLYPQTIARYFFVYNPRGCWDWWGYTGSQYATKAAPQIRAVLAMVERLGAPRN"
     gene            complement(1096..>2071)
                     /locus_tag="VGO02_12765"
     CDS             complement(1096..>2071)
                     /locus_tag="VGO02_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV7478597.1"
                     /translation="GFDAVVGAYASPYHPGTAYVLLHHCFGEVNGKRGIWGHAIGGMG
                     AITQALAAEARRWNVEIECNAPVQRVLSESVVLEDGTEIAARAVIANVNPKLLFLKLV
                     DSSRLERDFRERIERYKCGSATFRMNVALSALPRFSVLPEPGPHHGSGIIMAPSLEYM
                     DRAYLDARAEGIARRPIVEMLIPSTLDDSLAPKGAHVASLFCQHFAPDADWSSRKSEA
                     VERIFDVVESHAPGFRQSVIAHSALSPADLEREFGLVGGDIFHGQLSLDQLWSARPVL
                     GYADYRTPVPGLYLCGSGAHPGGGVTGAPGHNCAREVLRDLGRRFPGA"
BASE COUNT          354 a          676 c          712 g          329 t
ORIGIN      
        1 ctgctggccg gcatagggct ggtgctgtgc cagcgtatcc cccgccacga ggagggcgac
       61 gatgaccaat agtcttttca ctggattctc ctaaggaaac ttgtcaattg cgaggcgcgc
      121 cgaggcgctc gaccatcgcg agcacggcgc ggatctgcgg cgcggccttg gtggcgtatt
      181 gagaacccgt atagccccac cagtcccaac agccgcgcgg gttgtagacg aagaaatagc
      241 gcgcaatggt ttgcgggtag agcacgatca agcggttggt gtcggcccag cggttgtagc
      301 ccgcctggcg cacaaattgg tcaccgaccg cgtcagcgtt ctgccggcag ccatggaaga
      361 caacgtggac cgcgcagcgc gcgctcaagc acgccgtggg cacatagacg tagccgctgt
      421 ccgccatgct gatcgcgtag gggtttccat ccgcgaagga cttctgatcg aaggcgacca
      481 ggcggccgcc ctccttggac gccggcttga gcgggccgag cagctgcgcc agcagcgcgc
      541 ccgccgcgtc gtagtcgcag tcgttgatga acggtgcctc ggtggtgccg caggccgttc
      601 ccgcgtcttc ggtcaccatg gcatggcccg ccggcctggc gacgatcggt acgttcttgg
      661 cgccgaacag cgcatagaac cgcgccgcgg cttcgaccac cgggcgcgcg acggtgcggt
      721 cccgcgagcc cgaaaagaac cagagccgcg ttgccgcgag attcccggtg gagtcgatgc
      781 gcccttcgct cgcgaagtgc tcggcctgcg ccttgagcgc ctcgatgctc ggcagcggcg
      841 tccaactgcc gggtgtcgtg cagttgtagt acgcggccca caggctaccc tgcgcgcagt
      901 agtaggggcc ggcagccagc gcggcgacgc ccttgacgcg gccggaatgc gcgacctgga
      961 actggatcgc catgaagccg cctgaggaaa ggccggaaac agagaacgtc ttgacgtcgg
     1021 catgctctcc ggccagccgg tctgcagctc cagcgacgtg acagagcaga attcctgcaa
     1081 taaggaacag cgccttcacg ccccggggaa acgccggccg agatcgcgca gcacttcgcg
     1141 cgcgcagtta tgccccgggg cgccggtcac gccgccgccc gggtgcgcgc cggagccgca
     1201 caggtacagt cccgggacag gcgtgcggta gtccgcgtag ccgagcaccg gccgcgccga
     1261 ccagagctga tcgagcgaca gctggccatg gaagatgtcg ccacctacca ggccgaactc
     1321 gcgctcgagg tcggcgggcg agagcgcgct gtgagcgatc acgctctgcc ggaatccggg
     1381 ggcatgcgac tccaccacgt cgaagatgcg ttccactgct tcgctttttc gcgagctcca
     1441 gtcggcgtcg ggcgcgaagt gctggcagaa caggctggcg acgtgcgcgc ctttcggcgc
     1501 gaggctgtcg tcgagcgtgc tcgggatcag catctctacg atcggcctgc gggcgatgcc
     1561 ttcggcgcgg gcatcgaggt aggcgcgatc catgtattcc agcgacggcg ccatgatgat
     1621 gcccgagccg tgatgcggcc ccggctcggg cagcacggag aagcgcggca gcgctgacag
     1681 cgccacgttc atgcggaagg ttgccgagcc gcacttgtag cgctcgatgc gctcgcggaa
     1741 gtcgcgctct agccgcgagg aatcgaccag cttgaggaac agcagcttcg gattgacgtt
     1801 ggcgatcacg gcgcgcgcgg caatctcggt tccgtcttca agaactacgg attcggaaag
     1861 aacacgttga accggtgcgt tgcattcgat ctcgacgttc cacctgcgcg cttcggcggc
     1921 aagcgcttgc gtgatcgcgc ccatgccgcc gatggcgtgg ccccagatgc cgcgcttgcc
     1981 gttcacctcg ccgaagcagt ggtgcaggag cacgtaggcc gtgcccgggt gatacgggct
     2041 ggcataggcg ccgaccaccg cgtcgaagcc g
//
LOCUS       DASYJA010000583         3166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_360672, whole genome shotgun sequence.
ACCESSION   DASYJA010000583 DASYJA010000000
VERSION     DASYJA010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360672"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VGO02_12770"
     CDS             complement(<1..433)
                     /locus_tag="VGO02_12770"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV7478598.1"
                     /translation="MRLLNWNVQSCRGMDGRVDPARIAAETRRFGADVACFQEIDSDA
                     ILHALPDYQSAWASGVDVPAARGRFRFGNVILSRLPLGRVLRHSLPWPASPDTPSMPR
                     VAVEAVIGELRVITTHLEYYSAAHRAAQVDSLEKIFLEGKAE"
     gene            454..1419
                     /locus_tag="VGO02_12775"
     CDS             454..1419
                     /locus_tag="VGO02_12775"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEV7478599.1"
                     /translation="MREWLAILALAAAPALAQDTPEQTLSAVVAVNAKALPNARSAAT
                     LGTQRRGAGALIGDGYVLTIGYLVIESEAIEVTGADGKVVPATLAGYDHASGFGLLKL
                     AAPLAGRPLALGDSAALAEREPALVATTSEREGVSLVYVVSRRAFTGNWEYQLDSAIF
                     TYPAVDAWSGAPLIGPHGELLGIGSLIVPDAGGVGSQNPGNMFVPVELLKPILADLMK
                     SGRRSGAPRPWLGVNADEVRGRLFVSRLSPEGPGERAGLQPDDIVLAVAGEQVTTLAE
                     FYRRVWARGEAGVVVPIRVLRGVQLKDVSVRSIDRVEYFRASKGY"
     gene            complement(1416..2375)
                     /locus_tag="VGO02_12780"
     CDS             complement(1416..2375)
                     /locus_tag="VGO02_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478600.1"
                     /translation="MRRSDYDVTDSVRTTDPAAVGAEVLRIYRGLFNGRPVPELERAF
                     PDHAALYAGRHPDYLPCDTEYHDIQHVLDVTLAMARLMDGYHQSRRKDDAELPREVFV
                     IGTLAALFHDFGYLRRRHDHRHQFGAEYTLIHVSRGAAYLRRYVRELGLGPLAAVASQ
                     LVHYTGYERPAETIRVSDTLLRRVGQMLGTADIIAQMSDRCYLEKCRDRLFPEFVLGN
                     LAGRKVRGGRALPAFASGEELVQKTPGFYLGALKRLDLQLARAYEYAARHFGGPNLYL
                     DEMRKNVAYAQSVAADPSEGLRRHPPSTLAPEARPYPKDLMKL"
     gene            2514..2696
                     /locus_tag="VGO02_12785"
     CDS             2514..2696
                     /locus_tag="VGO02_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEV7478601.1"
                     /translation="MAGRLHELEVEHHDLDDVILRLAADRTLDQLQLQRLKKRKLFLK
                     DQIAKLRTRLIPDLTA"
     gene            2693..3145
                     /gene="dtd"
                     /locus_tag="VGO02_12790"
     CDS             2693..3145
                     /gene="dtd"
                     /locus_tag="VGO02_12790"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEV7478602.1"
                     /translation="MIALVQRVAEAKVTVGGETVGAIGRGILALVGVERGDGEAEAQR
                     LAEKVLAYRIFPDAAGKMNLSLQDIGGELLAVPQFTLAADTNSGTRASFSAAAAPPDA
                     QRLFAYFVERLKAKSRVATGTFGAGMQVSLVNDGPVTFWLQVSPQNKS"
BASE COUNT          498 a         1026 c         1139 g          503 t
ORIGIN      
        1 gctctgcttt tccttcgagg aagatttttt caagagaatc cacctgcgct gcgcggtgcg
       61 cggccgagta gtactcgagg tgcgtggtga tgacgcgcag ctcgccgatg actgcctcga
      121 ccgccacacg cggcatgctc ggcgtgtccg gcgaagcggg ccacggcagc gaatggcgca
      181 ggaccctgcc cagcggcagg cgcgagagga tcacattgcc gaagcggaag cggccgcgcg
      241 cggccggcac atcgacgccg gaagcccacg ccgactggta atccggcagg gcatgcagga
      301 tggcgtcgga gtcgatttcc tggaagcagg cgacgtcggc gccgaagcgc cgggtctcgg
      361 cggcgatgcg cgccgggtcg acccggccgt ccatgccgcg gcaggattgc acgttccagt
      421 tgagcaggcg catgatgcgt cgaggaggct cccatgcgtg aatggttggc tattctggca
      481 ttggcggcgg cgccggcgct cgcgcaggat acgcccgagc agactctctc cgcggtagtg
      541 gcggtcaacg ccaaggcgct gcccaatgcg cgcagcgcgg caaccctggg tacgcagcgt
      601 cgcggcgccg gcgcgctgat cggcgatggc tatgtgctca ccatcggcta cctggtgatc
      661 gaatccgaag cgatcgaagt gaccggggcc gacggcaagg tggtgccggc gacgctcgcc
      721 ggctacgacc acgcgtcggg cttcggcctg ctcaagctcg ccgcgccgct cgccggccgg
      781 ccgctggcgc tgggcgattc ggccgcgctg gccgagcgcg agccggcgct ggtcgccacc
      841 acatccgagc gcgagggcgt gagcctggtc tacgtcgtgt cgcgccgcgc gttcaccggg
      901 aactgggaat accagctcga ctcggcgatc ttcacctacc cggcggtgga cgcctggtcg
      961 ggggcgccgc tgatcggtcc gcacggcgag ctgctcggca tcggctcgct gatcgttccc
     1021 gacgcgggcg gcgtcggcag ccagaatccc ggcaacatgt tcgtgccggt ggagctgctc
     1081 aagccgatcc tcgcggacct gatgaaatcg ggcagacgca gcggggcgcc gcggccctgg
     1141 ctcggcgtca acgccgacga ggtgcgcggc cgtctcttcg tatcgcgctt gtcccccgag
     1201 gggcctggcg agcgcgccgg gctgcagccc gacgacatcg tgctcgccgt ggcaggcgag
     1261 caggtcacca cgctcgccga gttctaccgc cgcgtctggg cgcgcggcga ggccggcgtg
     1321 gtagtgccga tccgggtgct gcgcggggtg cagctcaagg acgtctcggt gcgctcgatc
     1381 gatcgcgtgg agtacttccg cgcttcgaag ggctactaga gcttcatcaa gtccttcggg
     1441 tacggcctcg cctcgggggc gagcgtgctc ggcgggtggc ggcgcaggcc ctcggaggga
     1501 tcggccgcca cgctctgcgc gtaggcgacg ttcttgcgca tctcgtcgag atagaggttc
     1561 ggcccgccga agtggcgcgc cgcgtactcg taggcacgcg ccagctgcag gtcgaggcgc
     1621 ttcagcgcgc ccaggtagaa gccgggcgtt ttctgcacca gctcctcgcc cgaggcgaag
     1681 gccggcagcg cgcgaccgcc gcgcaccttg cggccggcga ggttgcccag cacgaactcc
     1741 gggaacagcc ggtcgcggca tttctccagg taacagcggt ccgacatctg cgcgatgatg
     1801 tcggcggtgc cgagcatctg cccgacgcgc cgcagcaggg tgtccgatac gcggatcgtc
     1861 tccgccgggc gctcgtagcc ggtgtagtgc accagctggc tggccaccgc cgcgagcggc
     1921 ccgaggccga gctcgcgcac gtagcggcgc aggtaggcgg cgccacgcga tacatggatg
     1981 agcgtgtact cggcgccgaa ctggtggcgg tggtcgtgcc gccggcgcag gtaaccgaag
     2041 tcgtggaaca gggcggcaag ggtgccgatg acgaacacct cgcgcggcag ctcggcgtcg
     2101 tccttgcgcc gggactggtg gtagccgtcc atcaggcgcg ccatggccag cgtcacgtcc
     2161 agcacgtgct ggatgtcgtg gtactcggtg tcgcagggca ggtagtccgg gtgccggccc
     2221 gcgtagaggg cggcgtggtc cgggaaggcc cgctccagct cggggaccgg gcggccgtta
     2281 aacaggccgc gataaatgcg caaaacctcg gcgccaaccg ccgccggatc ggtggtccgg
     2341 acggagtcgg tgacgtcgta gtcgctgcgg cgcattggta acaggccttt ttctggggaa
     2401 ggggcctttt cggaaaccct agtataggga tacgattaac gggccgcaga gaagtttttc
     2461 acggaggggc agagttgacc gacctccctc ttaccgaaga ggaaaaagcc cagatggcgg
     2521 gccgcctgca tgagctcgag gttgagcacc atgaccttga cgacgtcatc ctcaggctgg
     2581 cggccgatcg cacgctggac cagctgcagc tgcagcggct gaagaagcgc aagctgttcc
     2641 tcaaggacca gatcgccaag ctgcgcaccc ggctgatccc cgacctcact gcgtgatcgc
     2701 tcttgtgcag cgcgtcgccg aggcgaaagt cacggtcggc ggcgagaccg tgggcgccat
     2761 cgggcgcggc atcctcgcgc tggtcggcgt cgagcgtggc gacggcgaag ccgaagcgca
     2821 gcgcctcgcg gaaaaagtcc ttgcttaccg gatattcccc gatgccgccg ggaagatgaa
     2881 tctttcgttg caggacatcg gcggcgagct tctcgcggtg ccgcaattca cgctggcggc
     2941 ggatacgaac tcgggcacgc gagcgagttt ctcggctgcc gccgcccctc cggacgcgca
     3001 gcggcttttc gcttatttcg tagaacgact aaaagcaaag agccgtgtgg ccacagggac
     3061 cttcggcgcc ggcatgcagg tcagcctcgt gaacgacggc ccggtgactt tctggctgca
     3121 ggtttctcca cagaataagt cttgaatttc gccggcgaga cgaact
//
LOCUS       DASYJA010000584         2725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_450292, whole genome shotgun sequence.
ACCESSION   DASYJA010000584 DASYJA010000000
VERSION     DASYJA010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450292"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1429)
                     /locus_tag="VGO02_12795"
     CDS             complement(<1..1429)
                     /locus_tag="VGO02_12795"
                     /inference="COORDINATES: protein motif:HMM:NF039586.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEV7478603.1"
                     /translation="MKWVLIIGGVVAALGLFLLATASGDTAVLERQYPLILGLNAALA
                     ALLAALVAYQLVAIVRRYRARVFGTRLTLRLLTRFAALAVVPGLIVYAVSVQFLTRSI
                     ESWFDVKVDAALEGGINLAQQAIDQMMLELQDKARAMALELAERSEPQLPARLERLRS
                     QAGVAEAVVVTASGRVVASASDDVTRLVPELPSRELLRQARANRGYTTVDSVAGRPLS
                     LRVVLPLSARGLAEEARYLQLRNTVPLQFGRSAEAVEVAYRDYRELAISRDGLKRIYV
                     VTLSFALLMALFVAVAVAATQSNLLAEPLANLAQATQAVARGDFSRQAPVTSRDELGV
                     LTESFNSMTHQLEDARRGVESNRVALEAAKAHLENILANLSAGVLVYDHELRMTISNH
                     GARAIAGAELDAFADRMRERFVEHGAKDWQLDLALRDGKTVHARGARLPLVTGGGYVA
                     VFDDITQLIQAQRAIAWAEVARRLAH"
     gene            complement(1426..2034)
                     /locus_tag="VGO02_12800"
     CDS             complement(1426..2034)
                     /locus_tag="VGO02_12800"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEV7478604.1"
                     /translation="MRRIAFLARLALLLASLAACHARADEIEVRDVSLRPTEEGLVLD
                     ADFAFELTPRLAEVVANGVPLYFRVEFELIRRRWYWFDDTTAVRRLQVRLSYHALSRQ
                     YRLSTGLLQQNFATLEEALNVLKRVRNWLVVERSVPLSGDYEAAVRMRHDVSLLPKPF
                     QLSALTARDLHLDSAWHRFTVRSLEQAPAPVESRDLQGGSPR"
     gene            complement(2044..>2725)
                     /locus_tag="VGO02_12805"
     CDS             complement(2044..>2725)
                     /locus_tag="VGO02_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEV7478605.1"
                     /translation="ASAVGDTALLLEKPVPVERLPGFADGDVSVQDAGAQRATGLLEL
                     APGLRVLDACAGPGGKSAHILERADVALTALDVDAARCERLTRNLERLSLRADVRAAD
                     CTRPQTWWDGAAFDRILADVPCTASGVARRHPDLKWLRRAGDASAFAARQAAILDALW
                     QLLAADGKLLYVTCSVFPQENQEVVQAFLARTQGARPLPLPDGGESQWLPGTRHDGFF
                     YALIQKQA"
BASE COUNT          427 a          969 c          968 g          361 t
ORIGIN      
        1 cgtgcgcgag ccggcgcgcg acctcggccc aggcgatcgc gcgctgcgcc tggatcagct
       61 gggtgatgtc gtcaaacacc gccacgtagc ctccgccggt gacgagcggc aggcgcgcgc
      121 cgcgcgcgtg caccgtcttg ccgtcccgca acgcaaggtc gagctgccag tccttcgcgc
      181 cgtgctcgac gaagcgctcg cgcatccggt cggcgaaggc gtcgagctcg gctccggcga
      241 tggcgcgcgc gccgtggttc gagatcgtca tgcgaagctc gtggtcgtac accagcacgc
      301 cggccgacag gttggccagg atgttctcga ggtgcgcctt cgcggcctcc agcgcgacgc
      361 ggttggattc gaccccgcgg cgcgcgtcct ccagctggtg cgtcatcgag ttgaacgatt
      421 cggtgagcac gccgagctcg tcgcggctcg tgaccggggc ctggcgcgag aaatcgcccc
      481 gcgccacggc ctgggtcgcc tgcgcgaggt tggcgagcgg ctccgcgagc aggttcgact
      541 gcgtggccgc gacagccacg gcgacgaaca gcgccatcag cagcgcgaag ctcagcgtca
      601 ccacgtagat gcgcttaagc ccatcgcgcg agatcgcaag ctcgcgatag tcgcggtagg
      661 cgacctcgac tgcctccgcg ctgcggccga actgcagcgg gacggtattg cgcagctgca
      721 ggtagcgcgc ttcctccgcc agcccgcgcg cgctgagcgg cagcaccacg cgcagcgaaa
      781 gcgggcggcc ggcgaccgaa tcgaccgtgg tatagccgcg gttggcgcgc gcctggcgca
      841 gcagctcgcg gctcggcagc tcgggcacga ggcgcgtgac gtcgtcgctc gcgctcgcga
      901 ccacgcgtcc gctcgcggtc acgaccaccg cctccgccac gcccgcctgg ctgcgcaggc
      961 gctccaggcg cgccgggagt tgcggctcgg agcgctcggc aagctcgagc gccatcgcgc
     1021 gcgccttgtc ctgcagctcg agcatcatct ggtcgatcgc ctgctgcgcc aggttgatcc
     1081 cgccctcgag cgccgcatcc accttgacgt cgaaccacga ctcgatcgag cgggtgagaa
     1141 actggaccga caccgcgtag acgatgaggc ccggcaccac cgcgagcgcg gcgaagcggg
     1201 tcagcagccg cagcgtgagc cgggtgccga aaacccgggc gcgatagcgc cggacaatcg
     1261 cgacgagctg gtaggcgacg agcgcggcga gcagcgcggc gagcgccgcg ttgagcccga
     1321 gaatgagcgg atactgccgc tcgagcaccg cggtgtcgcc gctcgcggtg gcgagcagga
     1381 acagtcccag cgccgccacc acgccgccga tgatcagcac ccacttcatc ggggcgagcc
     1441 tccctgcaga tcgcggctct ccaccggcgc cggcgcctgt tcgagcgagc gcacggtaaa
     1501 gcggtgccac gccgaatcga ggtggaggtc gcgcgcggtg agggcggaaa gctggaacgg
     1561 cttgggcagc agcgacacgt cgtggcgcat gcgcaccgcc gcctcgtagt cgcccgagag
     1621 cgggacactg cgctcgacca ccagccagtt gcgaacgcgc ttcaacacgt tgagcgcttc
     1681 ctccagcgtc gcgaagttct gctgcagcag ccccgtcgac agccggtact gccgcgacag
     1741 cgcatgatag gacagccgca cctgcagccg ccgcaccgcg gtcgtgtcgt cgaaccagta
     1801 ccagcggcgg cgtatcagct cgaactcgac gcggaagtag aggggcacgc cgttcgccac
     1861 tacctcggca agccgcggtg tgagctcgaa ggcgaaatcg gcgtcgagca ccagcccttc
     1921 ctcggtgggc cgcagcgaga cgtcgcgcac ctcgatctcg tcggcgcgcg cgtggcacgc
     1981 ggcgagcgag gcgaggagca gggcgagtct cgcgaggaag gcgatgcggc gcaccgcgtt
     2041 gcgtcaggct tgtttttgga tcagggcgta aaagaacccg tcgtgccggg tgcccggcag
     2101 ccactggctt tcgccgccgt cgggcaacgg caaaggccgc gcgccttgcg tgcgcgcgag
     2161 gaaagcctgc acgacttcct ggttctcctg cggaaatacc gagcaggtga catacagcaa
     2221 tttaccatcc gctgcgagca gctgccaaag cgcgtccaga atcgccgcct ggcgagccgc
     2281 gaacgccgac gcatcgccgg ctcggcgcag ccacttgagg tcgggatgac gccgcgcgac
     2341 gccggaggcc gtgcacggca cgtctgcgag gatgcggtcg aaggctgcgc catcccacca
     2401 ggtttgcggc cgcgtgcaat cggccgcgcg cacgtcggcg cgcagcgaaa gccgctcgag
     2461 gttgcgcgtg aggcgttcgc agcgcgcggc gtccacgtcg agcgcggtga gcgcgacgtc
     2521 ggcgcgctcc agaatatggg cgctttttcc gcccggcccg gcgcaggcgt cgagcacgcg
     2581 cagtcccggc gcgagctcga ggaggccggt cgcgcgctgc gcgcccgcgt cctggaccga
     2641 gacgtcgccg tcggcaaagc ccggcagccg ctcgaccggc accggcttct cgagcaacag
     2701 cgccgtgtcg cccaccgcgc tcgcg
//
LOCUS       DASYJA010000585         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1722433, whole genome shotgun sequence.
ACCESSION   DASYJA010000585 DASYJA010000000
VERSION     DASYJA010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1722433"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="VGO02_12810"
     CDS             <1..1003
                     /locus_tag="VGO02_12810"
                     /inference="COORDINATES: protein motif:HMM:NF023242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase C39 family protein"
                     /protein_id="HEV7478606.1"
                     /translation="QRFLRARSAEVLVAQDHTGLAGNAIVLFRPRSVIARLYSIAVVP
                     AMSGRGVGTMLLAAAEAAALARGCRAIRLEVHVTNHAAIARYRKSGYREFGRLRKYYE
                     DGGDALRFEKRLDLPAPKAAPLYIHQTTEFTCGPACMMMALAWADRARTPAPAALEFQ
                     LWREASTVFASSGPGGCEPFGVAVALKRRGLNPEIHVSRAGPYFLDGIRSADRRRVME
                     VTQLDFRRQAQALGIPTHLTPVNESVLMQAFDAGNVAIVLVSGYHMVPRGKPHWIFAF
                     GRDGRRVLMHDPAALRDDQGMAAAAETYAVPWTAFERVIHLGRERLSAAIVIRKGPS"
     gene            1000..>2069
                     /locus_tag="VGO02_12815"
     CDS             1000..>2069
                     /locus_tag="VGO02_12815"
                     /inference="COORDINATES: protein motif:HMM:NF025759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK-like ATPgrasp N-terminal domain-containing
                     protein"
                     /protein_id="HEV7478607.1"
                     /translation="MTGWVILVDQPKDLPNAETPHKVITTSEYLARPRLFDMGRPKLV
                     NLARSYAYQSKGYYASLLAEARGHRVVPTVETMLELREAKLYEHSLPELEDELNRCAR
                     RADYQPEGEFKLLVCFGIARDERFEQFGRLLFDWFRCPALEVNVEPGTWLSIERIRPR
                     NITRLANGEASFLREALHLHTKREWRDPKARTIAKYDLAVLYDPNEKMAPSSPASIKY
                     MARIAEKLSIDVEPVTKRQLAELAEYDGLFIRETTSIDNHTYRFARRAWQEGMPVIDD
                     PISMIRCTNKVFLMELLGQNQVATPPTVMLADAGDMPRAMDELGLPLVVKIPDGSFSR
                     GVHKVHTPEEFRRIADELFEET"
BASE COUNT          336 a          733 c          680 g          320 t
ORIGIN      
        1 gcagcgcttc ctgcgcgcgc gcagcgctga ggtgctggtg gcgcaggacc acaccggcct
       61 cgccggcaac gctatcgtgc tgttccgccc gcgctcggtg atcgcgcggc tctattccat
      121 cgccgtcgtg cccgccatga gcggccgcgg cgtgggcacg atgctgctgg cggcggccga
      181 ggcggcggcg ctcgcgcgcg gctgccgggc catccgcctc gaagtgcacg tcaccaacca
      241 cgccgcgatc gcgcgctaca ggaagagcgg ctaccgcgag ttcggccgcc tgcgcaaata
      301 ctacgaggac ggcggcgatg cgctgcggtt cgaaaagcgg ctcgacctgc ccgcgcccaa
      361 ggcggcgcct ctctacatcc accagaccac cgagttcacc tgcggcccgg cctgcatgat
      421 gatggcgctg gcctgggccg atcgcgcccg aacgccggcg ccggccgcgc tcgagttcca
      481 gctctggcgc gaagccagca ccgtgttcgc gagctccggc ccgggcggct gcgagccctt
      541 cggtgtggcg gtcgcgctca agcgccgcgg cctgaacccc gagatccatg tgagccgcgc
      601 cggcccgtac ttcctcgacg ggatcaggtc ggccgaccgg cgccgggtca tggaggtgac
      661 gcagctcgac ttccggcgcc aggcgcaggc gctcggcatc cccacccacc tgactcctgt
      721 gaacgaatca gttctgatgc aggcattcga cgcggggaac gtggccatcg tcctcgtttc
      781 gggctatcac atggtcccca gaggaaagcc gcactggatt ttcgccttcg gccgcgacgg
      841 acgtcgtgtc ctcatgcacg atcccgcggc gctgagagat gatcaaggga tggcggccgc
      901 tgccgagact tacgcggtgc cgtggaccgc gttcgagcgc gtgatccatt tgggtcgcga
      961 gcggctgagc gcggccatcg tcatccggaa aggaccttca tgaccggctg ggtcattctg
     1021 gtcgaccagc ccaaagacct tcccaacgct gagaccccgc acaaggtgat cacgacgagc
     1081 gagtatctcg cccgtccgcg cctgttcgac atgggccggc cgaagctggt caacctggcg
     1141 cgctcctacg cctatcagtc caagggctac tacgcctcgc tgctggcgga agcgcgcggc
     1201 caccgcgtgg tgccgacggt cgagaccatg ctggagctgc gcgaggccaa gctctacgag
     1261 cactcgctgc cggagctcga ggacgagctc aatcgctgcg cgcgccgcgc cgactaccag
     1321 cccgagggcg agttcaagct gctggtctgc ttcggcatcg cccgcgacga gcgcttcgag
     1381 cagttcgggc ggctgctgtt cgactggttc cgctgcccgg cgctcgaggt caatgtcgag
     1441 cccggcacct ggctgtcgat cgagcgcatc cggccgcgca acatcacgcg cctcgccaac
     1501 ggcgaagcga gcttcctgcg cgaggcgctg catcttcaca ccaagcgcga atggcgcgac
     1561 cccaaggccc gcacgatcgc gaaatacgac ctcgcggtgc tgtatgatcc caacgagaag
     1621 atggcgccgt cgtcgccggc ctcgatcaag tacatggcgc gcatcgccga gaagctgtcg
     1681 atcgacgtcg agccggtgac gaagcgccag ctcgccgagc tcgccgaata cgacggcctg
     1741 ttcatccgcg agaccacgtc gatcgacaat cacacctacc gcttcgcgcg ccgcgcctgg
     1801 caggagggca tgccggtcat cgacgacccc atctccatga tccgctgcac caacaaggta
     1861 ttcctcatgg agctcttggg ccagaaccag gtggcgacgc cgccgaccgt gatgctggcc
     1921 gatgccggcg acatgccgcg cgcgatggac gagctcggcc tgccgctggt ggtgaagatc
     1981 cccgacggct cgttctcgcg cggtgtgcac aaggtacaca cgccggagga gttccgccgc
     2041 atcgccgacg agctgttcga ggagaccga
//
LOCUS       DASYJA010000586         3782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_270052, whole genome shotgun sequence.
ACCESSION   DASYJA010000586 DASYJA010000000
VERSION     DASYJA010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270052"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VGO02_12820"
     CDS             <1..840
                     /locus_tag="VGO02_12820"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEV7478608.1"
                     /translation="LRAKTFTAQLRTLSGVIAEQGLDRIDLLKINVEKSELDVLQGIS
                     AGDWQKIHQLVIEVDLKQNLQPVTALLEQHGFEWVVEQDPLLKKTELCYVYAIRPSAE
                     RPGLVRQQAAGAHVRPVPSWNEEILTPPSLRHFLRERLPQYMIPSAFVLMEKVPLTSN
                     GKIDRQGFPAVAHEKAPPSPAADRPQTETEKALAAIWADLLKVESVGLHEDFFDLGGH
                     SLLAIRSIARIRDTFEVDLQLRNLFECPTVAGLAAVIDGLKWLAKPKAPLDGAGRREE
                     LVL"
     gene            840..1853
                     /locus_tag="VGO02_12825"
     CDS             840..1853
                     /locus_tag="VGO02_12825"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HEV7478609.1"
                     /translation="MVSDFLAELGRKDIHVWGDGERLRCNAPEGAVTPDLRDQLRDRK
                     REILEFLRTAEAVARQQPAIVPLQPRGARTPIYGVPGHPGDVFCFSDLVKRLGDDQPF
                     YGLEPPGLDGRRQPMDRVDDIAAYFADQIQAFQPEGPYIIAGYCSGGATAFELARRLV
                     QRGSTVPLLAMFGTPHPSAFRPLPRLAFNLRYWAGRVLLHVREIAKMPSFEARRRYLG
                     ERFRARLKALNEAKGPATPDPVLARRARLSSAAITAVRRYAPLRYSGRVCLYLPNEAW
                     LRSGTAPLRWRSAAPNAETYFGPDTCNGLLMLLEPDAPAFAELFRQSLAKEPAFSRGP
                     VMR"
     gene            1873..3531
                     /locus_tag="VGO02_12830"
     CDS             1873..3531
                     /locus_tag="VGO02_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478610.1"
                     /translation="MRRPRFWLAVGLSIGLLGLAGMLAARWAVDATAAPASGGPAFQD
                     RLRSPIGTNLEALSYWSPQVPFVDVMKSSSEWLSGDAKDWGGGPPLDLDANGWVRSLR
                     PGQMARKLMLREFGDRYPGGRYLVRYKGEGNLRLGFAAKVVSQRTGEMLIEVTPGDAG
                     VYLWIDATTPANYLREIEILMPGGICEGDPFRHARSAHDCGTRRFISFAQDRSIIFYP
                     VFAERLRGYSVLRFMDWMRTNSSPVATWPQRTPVTYHTWATDTGAPMEVMIALANLVG
                     AHPWFTLPHQSDEAYARSFAQLAKEKLDPALGVYVEHSNEVWNSQFAQYGYLTNRAKA
                     GGASLFQYHALRTRTLAAVFKAELGAERVVSVLGGQAANPGVATSGLDYLKGRFGAAS
                     LGIDAVAIAPYFGVTPMPAQAGRFTTMSLDAFIDYVRTNVFLQESAHMRNHRTVAGKY
                     GLRLLAYEGGQHMVGVAGAEGNAALNTLFDNFNRDARIKQLYLDYLALWKREGGELFV
                     HFNDVSRYTKSGRWGALEYVAQPRSESPKFDALQTFIEQNPAWW"
BASE COUNT          625 a         1269 c         1253 g          635 t
ORIGIN      
        1 cttcgcgcca aaaccttcac cgcccaactg cggaccctgt ccggcgtcat cgccgagcag
       61 ggcctcgacc gcatcgacct gctcaagatc aatgtggaga aaagcgagct ggatgtcctg
      121 cagggcatca gcgccggcga ctggcagaag atccatcaac tggtcatcga ggtggacctg
      181 aaacagaacc tgcagccggt cacggccctg ctggagcagc acggcttcga gtgggtcgtg
      241 gaacaggacc cgttgctgaa aaagaccgag ctctgctacg tgtatgcgat ccggccctcg
      301 gcggagagac ccggcctcgt ccggcagcag gccgccggcg cgcacgtgcg gccggttccc
      361 tcgtggaatg aagagatcct cacgccgcct tcgctgcgcc acttcctgcg cgagcgcctg
      421 ccccagtaca tgattccttc ggcgttcgtc ctgatggaga aggttccgct gacgtcgaac
      481 ggcaagatcg atcgccaggg gtttccggcg gtcgctcacg agaaggcgcc gccctccccg
      541 gccgcagacc ggccgcagac ggagactgaa aaagcgctcg ccgcgatctg ggccgatttg
      601 ctgaaggtgg agagcgtcgg ccttcacgag gatttcttcg atctgggtgg ccattccctg
      661 ctggcgatcc ggtccatcgc gcgcatccgg gacacgttcg aggttgacct gcagctgcgc
      721 aatctgttcg agtgcccgac ggtggcggga ttggcggcgg tcatcgacgg attgaaatgg
      781 ctggcgaagc ccaaggcgcc gctcgacggc gcggggcgtc gagaggagct ggtactttga
      841 tggtgtctga tttcctcgcc gagctgggcc gcaaggacat tcatgtctgg ggcgacggcg
      901 agcggctgag gtgcaacgct cccgagggcg cggtgacgcc ggatctgcgc gatcagctgc
      961 gggaccgcaa gcgcgagatt ctggagtttc tgcgaaccgc cgaggcggtc gccaggcagc
     1021 agccagccat cgtgccgctg cagccgcgag gcgcgcgaac gcccatctac ggcgtgcccg
     1081 gacatcctgg cgacgtcttc tgcttctccg acctcgtgaa gcggctgggc gacgatcagc
     1141 ccttctacgg gctggaaccc cccggcctcg acggccggag gcagccgatg gatcgcgtcg
     1201 acgacatcgc cgcgtatttc gctgaccaga tccaagcctt ccagccggaa ggcccgtaca
     1261 tcatcgccgg ctattgctcg ggcggcgcga ccgccttcga gctggcgcgc aggctcgtgc
     1321 agcgaggctc gacggtcccg cttctggcca tgtttggaac cccccatccc tccgcgttcc
     1381 ggccgctgcc cagactggcg ttcaacctgc gctactgggc cgggcgcgtc ctcctgcatg
     1441 tccgagaaat cgcgaaaatg ccgtctttcg aggcgcgccg gaggtacctt ggcgagcggt
     1501 ttcgggcccg cctgaaggcg ctgaacgagg ccaagggccc cgcgactccg gatccggtcc
     1561 tggcccggcg cgccaggctc tcgagcgcgg ccatcacggc ggtgcgccgt tacgctcccc
     1621 tgcggtattc cggacgggtg tgcctctacc tgccgaacga ggcgtggctg cgatccggca
     1681 ccgcgcccct gcgctggaga tcggcggcgc cgaatgccga gacttatttc ggaccggata
     1741 cctgcaacgg cctcctcatg ctcctcgagc ccgacgcgcc ggccttcgcg gaactcttca
     1801 gacagagcct cgcgaaggag ccggccttct cgcgcggccc ggtcatgagg tgactttcgg
     1861 cgcggagcag caatgagaag gccgcgattc tggctggccg tggggctatc gatcggcctg
     1921 cttgggctgg caggcatgct ggctgcgcgg tgggccgtcg acgccacggc cgcgccggcg
     1981 agcggcggtc cggcgtttca agaccggctg cgctcgccga tcgggacgaa tctggaggcg
     2041 ctgtcctact ggtcgccgca ggtgccgttc gtcgacgtga tgaagtcctc cagcgaatgg
     2101 ctttcggggg acgcgaaaga ctggggcggg ggcccgccac tggatctcga tgccaacggc
     2161 tgggtgcgct cgctgcggcc cgggcagatg gcacgcaagc tcatgctgcg cgagttcggc
     2221 gatcgctacc ccggcggccg ctacctcgtg cgctacaagg gcgaaggcaa tctccgcctc
     2281 ggcttcgccg ccaaagtggt ctcccagcgc actggcgaaa tgctgatcga ggtgacgcct
     2341 ggcgacgctg gcgtctatct gtggatcgac gcgactaccc cagcgaacta cctgcgcgag
     2401 atcgagatcc tcatgcccgg cggcatctgc gaaggcgacc cgttcaggca cgcgcgctcg
     2461 gcgcatgact gcggcacgcg gcgcttcatc tccttcgccc aggatcgcag catcattttc
     2521 tacccggtgt tcgccgagcg gctgcgcggc tactcggtgc tgcgcttcat ggactggatg
     2581 cgcaccaata gcagcccggt ggcgacctgg ccgcagcgta cgccggtgac ctaccatacc
     2641 tgggccacgg acacgggtgc gccgatggaa gtgatgatcg cgctcgccaa cctggtgggg
     2701 gcccatccgt ggttcacgct gccgcaccag agcgacgagg cctacgcgcg cagctttgcg
     2761 caactggcta aagagaagct cgacccggcg ctcggcgtgt acgtagagca ctcgaacgag
     2821 gtgtggaact cccagttcgc gcagtatggc tatctgacga atcgcgccaa ggccgggggg
     2881 gcaagccttt tccaatacca cgcgctgcgc acgcgcacgc tcgccgcggt gttcaaagcc
     2941 gaactcggcg ccgagcgcgt ggtctcggtg ctcggcggcc aggcggccaa tcccggggtg
     3001 gcgacgagcg ggctcgacta tctcaaaggc cgcttcggtg ccgcctcgct cgggatcgat
     3061 gcagtggcga tcgcgccata cttcggcgtc acgccgatgc ccgcccaggc cggcaggttc
     3121 accaccatga gcctcgatgc ctttatcgac tacgtgcgaa ccaacgtgtt cctccaagag
     3181 agcgcccata tgcgcaacca tcgcacggtg gccggcaagt acggcctgcg cctgctcgcc
     3241 tatgagggtg gccagcacat ggtcggtgtc gcgggcgcgg agggcaacgc ggcgctgaac
     3301 acgctcttcg acaatttcaa ccgcgacgcg cgcatcaagc agctctacct cgactacctc
     3361 gccctctgga agcgcgaggg cggcgagctc ttcgtgcact tcaacgacgt cagccggtat
     3421 acgaagtcgg gccgctgggg cgcgctcgag tacgtcgcgc agccgcgcag cgagtcgccc
     3481 aagttcgacg cgctgcagac cttcatcgag caaaaccccg cctggtggtg agggcctagc
     3541 caggatttcg ccgcgcggcc cagttgaccg cgccggcgcg gcgccgcgta gacgacgtcg
     3601 agctcgacgt aaacgacctg ccgggcctag cgattgcatg caccttgcgc aggtccacgc
     3661 cgatgctcaa agacgttcgc ccgccgctaa gtgcatttgt acgcagtgac gaatcgcacg
     3721 atgcccgatt aatacctatc gatgctcgtg aagaagttca tacgcttccc cgacgccgag
     3781 tg
//
LOCUS       DASYJA010000587         4124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_178512, whole genome shotgun sequence.
ACCESSION   DASYJA010000587 DASYJA010000000
VERSION     DASYJA010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178512"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..451
                     /locus_tag="VGO02_12835"
     CDS             17..451
                     /locus_tag="VGO02_12835"
                     /inference="COORDINATES: protein motif:HMM:NF021941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478611.1"
                     /translation="MTVAARNQRNEAKAPPSEKVEAISAGPAQIISFIPYVKMLARLS
                     DIQRVSESELNKIDAPMAVIGNTKLKLKIEVDLGAERERVAKEIARIEAQIAQTEGKL
                     SNETFVQRAPPKVVEEFRARLAEFRSTIGKLKEQQEKLTARA"
     gene            448..1701
                     /locus_tag="VGO02_12840"
     CDS             448..1701
                     /locus_tag="VGO02_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV7478612.1"
                     /translation="MNLPAGLRALRHRDFRLFFVGQGVSQMGTWLQLIATSWLVYRLS
                     GSTFLLGLSAFAMQISFLVMAPLAGVFVDRLDRRRVLIVTNSIAVLQAATMFWLVALG
                     HVAPWHLIAGNLVLGIVSACDAPARQSILIQLVGGKADLPNAIALNSVMMNGARFVGP
                     MVGGGVIAAFGEKWGFGVNALSYFVMIGALARMTPSPQPRARSEGSSLWSELAAGARY
                     AYGFLPSRCALLLLTATSLSVNSYAALMPWFARETFQGDSGVLGLLLGAAGFGAVSGM
                     VYLASRPGIRGLFRLIGWTAASAGAALVVFSFSSKLWLALPALYFVGMGLMLTAASTN
                     TVLQSIVPDELRGRVASLYVMSFLGMSPLGALAAGWAAEHIGPPATLAICGGVALAAA
                     AVYALQLPAIRREIRPEYQRLGVID"
     gene            complement(1698..2321)
                     /locus_tag="VGO02_12845"
     CDS             complement(1698..2321)
                     /locus_tag="VGO02_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478613.1"
                     /translation="MKRRDLLKGTAALLLIREALAQGRLEKGVYRLRGDVHVNGANAQ
                     QGGELRPGDTVRTQPGAEIVFVAQNDAFLVRQNAEVSLVANGLRVVTGAVLSVFAPGQ
                     PKEIRTSTAVIGIRGTAVYIEAEPQRTYACTCYGEAVLTPIAQPAAAETVRTTHHEAP
                     RYIMGSGAPQMVTVAPVINHTDAELILLEGLVGRQPPFIGQGFRPYN"
     gene            complement(2323..2832)
                     /locus_tag="VGO02_12850"
     CDS             complement(2323..2832)
                     /locus_tag="VGO02_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478614.1"
                     /translation="MDYEIIRRMMARWIRLGSPGPAEFEAACARLARAQAEGAAPILA
                     WAQGDTRHLFALIAPLKYAPGRRQRWFSWAAASAAATYRQFGLPAYVEGEALWLHGRK
                     IADGAVSTVGECALIASSFLARFPEQCVATPPPDLEQAFRLRLEAQHGWQFDTSWPTE
                     SETLHYALA"
     gene            2854..3018
                     /locus_tag="VGO02_12855"
     CDS             2854..3018
                     /locus_tag="VGO02_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478615.1"
                     /translation="MIPMSNQEIKDFWHGFCERQKVSAELRAKGDKEIDKDPEHWADH
                     TMWELLDAVK"
     gene            complement(3059..3874)
                     /locus_tag="VGO02_12860"
     CDS             complement(3059..3874)
                     /locus_tag="VGO02_12860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HEV7478616.1"
                     /translation="MEALKLNLKSGTYANPDQCRILFGALLVALQLQGRYEEIGAALR
                     AQWSPVREEGNERFDRLYMAALAATRSRVLPLGRRVRFQKLTEYLSSSRGAAGEVVEC
                     GCFMGLSTHLICQVLMEERPDFDGSGFHVFDSFEGLPEPTPEDAIPDDHPDAVRLRSM
                     SVKGRFAAPLDLVRSSLSRFPRVSYHVGWIPETFDGLPDASYRFVHLDVDLYQPTYDA
                     LDYFCGRLAPGGMIVCDDYSWPGARLAVDAYAKQHGLSLDLPATGQAAIRLPL"
     gene            complement(3978..>4124)
                     /locus_tag="VGO02_12865"
     CDS             complement(3978..>4124)
                     /locus_tag="VGO02_12865"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEV7478617.1"
                     /translation="VAAVAGGLMIEAGGSLLGAIGVSGAPGGKKDEMCAAAGIAAIRE
                     DIEL"
BASE COUNT          700 a         1398 c         1331 g          695 t
ORIGIN      
        1 cgccgcgctc aaggaattga ccgtcgccgc cagaaaccag aggaatgaag ccaaagcgcc
       61 tccgtccgag aaggtcgagg cgatcagcgc cggcccggcg cagatcattt ccttcattcc
      121 ctacgtcaag atgctggcca gactttctga cattcagaga gtcagcgaaa gcgaattgaa
      181 caagatagac gcgccgatgg ctgtcatcgg caataccaag ctcaaactga agatcgaagt
      241 cgacctagga gccgagcgcg agcgcgtggc gaaggagatc gcccgcatcg aagcccagat
      301 cgcgcagacc gaaggcaagc tgtcgaacga aacgttcgtg cagcgtgcgc ctcccaaggt
      361 ggtcgaggaa ttccgcgcac gcctggccga attcagatca accatcggca agctcaaaga
      421 acagcaagaa aagcttacgg cgcgcgcgtg aacctgccgg caggcctgcg agccctccgc
      481 caccgcgact ttcgcctctt cttcgtcggt cagggcgtct cgcagatggg cacctggctg
      541 cagctgatcg ccaccagctg gctggtgtac cgcctgtcag gctcgacctt cctgctcggc
      601 ctgtcggcct tcgccatgca gatctcgttc ctcgtgatgg cgccgctcgc cggggtgttc
      661 gtcgaccggc tcgaccggcg ccgcgtgctg atcgtcacca actcgatagc cgtgctgcag
      721 gcggcgacca tgttctggct ggtggcgctc ggccatgtgg cgccctggca cctgatcgcc
      781 ggcaacctgg tgctcggcat cgtcagcgcg tgcgacgcgc cggcgcgcca gtcgatcctg
      841 atccagctgg tcggcggcaa ggccgacctg ccgaacgcca tcgccctaaa ttccgtgatg
      901 atgaacggcg cgcgcttcgt cggcccgatg gtcgggggcg gcgtcatcgc ggcgttcggc
      961 gaaaaatggg gcttcggcgt gaatgcgctg tcctatttcg tcatgatcgg cgcgctcgcg
     1021 cgcatgacgc cctcgccgca gccgcgcgcg cgcagcgaag gttcaagcct gtggagcgag
     1081 ctggccgccg gcgcgcgcta cgcctacggc ttcctgccgt cgcgctgcgc gctgctgctg
     1141 ctcaccgcga ccagcctgag cgtcaactcc tacgccgcgc tcatgccgtg gttcgcgcgc
     1201 gagacttttc agggcgattc gggcgtgctc gggctgctgc tcggcgcggc ggggttcggc
     1261 gccgtctccg gcatggtcta cctcgcgtcg cggcccggca tccgcggcct gttccgcctg
     1321 atcggctgga ccgcggcgag cgccggcgcc gcgctggtcg tgttctcgtt cagctcgaag
     1381 ctgtggctcg cgctgcccgc gctgtacttc gtcggcatgg ggctcatgct cacggcggcc
     1441 tcgaccaaca ccgtgctgca gagcatcgtg cccgacgagc tgcgcgggcg cgtcgcctcg
     1501 ctctacgtca tgtccttcct tggcatgtcg ccgctcggcg cgctcgccgc gggctgggcg
     1561 gcggagcaca tcgggccgcc cgccacgctt gccatctgcg gcggcgtcgc gcttgccgcg
     1621 gccgcggtct atgcgctgca actgccggcg atccggcgcg agatccggcc ggagtaccag
     1681 cgcctcggcg tgatcgacta gttgtagggt cggaagccct ggccgatgaa cggcggctgg
     1741 cggccgacca ggccttccag caggatcaac tcggcgtccg tgtgattgat caccggtgcc
     1801 accgtcacca tctgcggggc gccgctgccc atgatgtagc gcggcgcctc gtggtgcgtg
     1861 gtgcgcacgg tttccgccgc cgcgggctgg gcgatgggcg tgaggaccgc ctcgccgtag
     1921 cacgtgcagg cgtaggtgcg ctgcggctcg gcttcgatgt acaccgccgt gccgcgaata
     1981 ccgatcactg ccgtcgatgt gcggatttcc ttgggttgac ctggagcaaa caccgagagg
     2041 accgctcccg tgaccacgcg caatccgttc gccaccagcg agacctcggc gttctggcgc
     2101 accaggaacg cgtcgttctg ggcgacgaag acgatctcgg cgccgggctg ggtacgcacc
     2161 gtgtcgccgg gacgcagctc gccaccctgc tgcgcgttgg cgccgttgac atgcacatcg
     2221 ccgcgcaggc ggtagacgcc cttctccagg cgcccctgcg ccagagcttc gcgtatcagg
     2281 agaagcgcag ccgtgccttt cagcaggtcg cggcgcttca cgtcaggcga gagcgtagtg
     2341 cagggtttcg gactcggtcg gccaggaagt gtcgaactgc cagccgtgct gcgcctccag
     2401 gcgcaggcgg aacgcctgct cgaggtcggg cggcggcgtc gcgacgcact gctccgggaa
     2461 gcgtgcgagg aagctggagg cgatcagcgc gcactcgccg accgtgctta ccgcgccgtc
     2521 ggcgatcttg cggccgtgca gccacaacgc ctcgccttcg acataggccg gcaggccgaa
     2581 ctggcggtag gtggcggccg cgctcgccgc ggcccacgag aaccagcgct ggcggcggcc
     2641 cggcgcgtac ttgagcggcg cgatcagcgc gaagagatgg cgggtatcgc cttgcgccca
     2701 cgccaagatc ggcgcggcgc cctcggcctg cgcccttgcc agccgcgcac aggccgcctc
     2761 gaactctgcg ggccccggcg aaccgagccg tatccagcgc gccatcatgc gccggattat
     2821 ttcataatcc acgccttaaa ttaggaggcg gcgatgatcc cgatgagcaa ccaggagatc
     2881 aaggatttct ggcacggttt ctgcgagcgc cagaaagtct ccgccgaatt gcgcgccaag
     2941 ggcgacaaag agattgacaa agacccggag cactgggccg accacaccat gtgggagctg
     3001 ctcgacgcgg tgaagtagat tccctacagt ccccaccagg gctcagttgc gtgcaacttc
     3061 agagcggcag acgaatcgcg gcctgtcccg ttgcgggtaa gtccaagctt agcccgtgct
     3121 gctttgcata tgcgtcgacc gccaagcggg caccgggcca actgtaatcg tcgcagacaa
     3181 tcatgccgcc cggcgcgagc cggccacaga agtagtccag cgcatcgtaa gtcggctgat
     3241 agaggtcgac gtcgaggtgc acgaaccggt agctcgcatc gggaagcccg tcaaaggtct
     3301 ccgggatcca cccgacatga taggaaactc gcggaaatcg gctcaggctc gagcggacaa
     3361 gatcgagcgg cgccgcgaat cttcccttta cgctcatgct gcgcaatctc acggcatccg
     3421 gatggtcgtc cgggatcgca tcctccgggg tcggctccgg cagtccctcg aacgaatcga
     3481 aaacgtggaa accgctgccg tcgaaatccg gccgttcttc catcaagacc tggcaaatca
     3541 agtgcgtaga caagcccatg aaacacccgc attccacgac ctcaccggcc gcgccgcggg
     3601 agctcgacag gtattccgta agcttctgaa atcgtactcg tctcccaagc gggaggacgc
     3661 gcgagcgcgt tgcagccagc gccgccatgt atagccggtc gaagcgttcg ttgccttcct
     3721 cgcgtaccgg cgaccattgc gctcgcaatg cggcgccgat ttcctcgtaa cggccctgaa
     3781 gctgcagcgc aaccagaagc gctccgaaca gaatccggca ttgatcaggg ttggcatacg
     3841 tgcccgattt cagattgagt ttgagcgctt ccaccaaaga tatgggatcg ccgccaaacg
     3901 cagccagaga gtccgcccgt attccagctt caaccagttg cctgaagagc ttcttaggta
     3961 tcacccggca aactcagcta cagctcgatg tcctcgcgga tcgcggcgat gcccgctgcg
     4021 gcacacattt cgtctttttt gccgccgggg gcgcccgaca cgccgatcgc gccgagcagc
     4081 gagccgcccg cctcgatcat cagcccgccg gcgaccgcgg cgac
//
LOCUS       DASYJA010000588         2269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1906231, whole genome shotgun sequence.
ACCESSION   DASYJA010000588 DASYJA010000000
VERSION     DASYJA010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1906231"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1741)
                     /locus_tag="VGO02_12870"
     CDS             complement(<1..1741)
                     /locus_tag="VGO02_12870"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEV7478618.1"
                     /translation="MTKILCALVAALVLLGCAANDTMHEGQQMIQAGNTEEGLARLEE
                     AYRKDPNDGEIRAAYLRNRQAAVERFLMQGDAQRNVGAWDQAEAAYGKALRFEPDNAR
                     AKAGVAAVALDKKSQTMLAEAEAALKAGDEKLALEKARAVLADNPSHRGAKSLIRKVE
                     QKEVRAESMSPKLQAALKKPITIELRDAPVRNVFELISQRSGMNFVYDKDIPADARAT
                     VHVRDTSIEDVMKFVMVTNQLDQRVLNENTLLIYPNTPQKNTAYKELVMKSFFLANAD
                     VKQTANMVRSLVKTRDIFVDEKLNLLVVRDTPEAIRAVEKLLASHDIGDPEVMLEVEV
                     LEVGYNTLTNLGIQWPGSAALSVIGAAGVPGQITGTEAKNFSSANTRLSFSDPLLALN
                     LRQQAGRTNVLANPRIRVKSKEKARIHIGDKVPVITTTAGATGFVSESVNYLDVGLKL
                     EVEPVVNLDDDVGIKVGLEVSNISGQSTTAAGTTSYTVGTRNAATTLRLHDGETQVLA
                     GLINDEDRRAATQVPGLSKMPVLGRLFKSTDDNITKTEIVLLMTPRVVRNIERPSARI
                     EHFVSGTDAEVGGA"
     gene            complement(1738..2172)
                     /locus_tag="VGO02_12875"
     CDS             complement(1738..2172)
                     /locus_tag="VGO02_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478619.1"
                     /translation="MKVAFAVLTLALLAGVVVGREKPMENLLSEKRSAETDFDVSRLE
                     KREAEAPASDPFAQRSFGAPQGEQVAVQAKPEAPALPFRYMGKVIEDGKLEILLLRGD
                     EHFAVKAGQKIDAEYRLDKVTASALTFTYLPMKTKQTLELIP"
BASE COUNT          294 a          749 c          775 g          451 t
ORIGIN      
        1 ccgcgccgcc gacttccgcg tcggtgcccg agacgaagtg ctcgatgcgg gccgacgggc
       61 gctcgatgtt gcgcaccacg cgcggcgtca tgagcaggac gatttccgtc ttggtgatgt
      121 tgtcgtcggt gctcttgaac aggcggccga gcaccggcat cttcgacagc cccggcacct
      181 gggtggcggc gcggcggtcc tcgtcgttga tcaggcccgc cagcacctgc gtctcgccgt
      241 cgtgcaggcg cagggtggtg gcggcgttgc gcgtgcccac cgtgtaggag gtggtacccg
      301 cggcggtagt gctctgcccg gagatattgc tcacctcgag gccgaccttg atgccgacgt
      361 cgtcgtccag gttgaccacc ggctcgacct cgagcttcag gccgacgtcg aggtagttca
      421 ccgactccga gacgaagccg gtggcgccgg cggtggtggt gatcaccggc accttgtcgc
      481 cgatgtggat gcgcgccttc tccttgctct tgacgcggat gcgcgggttg gcgagcacgt
      541 tggtgcggcc ggcctgctgg cgcaggttga gggcgagcag cgggtcggag aagctcaggc
      601 gcgtgttggc gctgctgaag ttcttcgcct cggtgccggt gatctggccg ggcacgccgg
      661 cggcgccgat cacgctcagg gccgccgagc cgggccactg gatgccgagg ttggtgagcg
      721 tgttgtagcc gacttccagc acctcgacct cgagcatcac ttccgggtcg ccgatgtcgt
      781 ggctggcgag cagcttttcc accgcgcgga tcgcctcggg ggtgtcgcgc accaccagca
      841 ggttgagctt ctcgtcgacg aagatgtcgc gcgtcttcac caggctcctc accatgttgg
      901 cggtctgctt cacgtcggcg ttggcgagga agaagctctt catcaccagc tccttgtagg
      961 ccgtgttctt ctgcggcgtg ttcgggtaga tgagcagcgt gttctcgttc agcacgcgct
     1021 ggtcgagctg gttggtgacc atcacgaact tcatcacgtc ctcgatcgag gtgtcgcgca
     1081 cgtgcaccgt ggcgcgcgcg tcggccggga tgtccttgtc gtagacgaag ttcatgccgc
     1141 tgcgctgcga gatcagctcg aacacgttgc gcaccggcgc gtcgcgcagc tcgatggtga
     1201 tcggcttctt cagcgccgcc tgcagcttcg gcgacatgct ctcggcgcgc acctctttct
     1261 gctcgacctt gcggatcagc gacttggcgc cgcggtggga cgggttgtcg gccaggacgg
     1321 cgcgcgcctt ctccagcgcg agcttctcgt cgccggcctt gagggcggct tccgcctccg
     1381 ccagcatggt ttgggatttc ttatccagag ccacagcagc aacgccggcc ttggcccgcg
     1441 cgttgtcggg ctcgaaacgc agcgccttgc cgtaagccgc ctcggcctgg tcccacgcgc
     1501 cgacgttgcg ctgggcgtcg ccctgcatga ggaagcgctc gaccgccgcc tggcggttgc
     1561 gcaggtaggc ggcgcggatc tcgccgtcgt tcggatcctt cctgtacgcc tcctccaggc
     1621 gcgccaggcc ctcctcggtg ttgcccgcct ggatcatttg ctggccctcg tgcatggtgt
     1681 cgttcgccgc gcagccgaga aggacgagcg ccgcaaccag ggcgcagagg attttcgtca
     1741 tggtatgagc tccagtgtct gtttcgtctt catcggcagg taggtgaagg tgagcgccga
     1801 ggcggtcacc ttgtcgaggc ggtactcggc gtcgatcttc tgcccggcct tgacggcgaa
     1861 gtgctcgtcg ccgcgcagca gcaggatctc cagcttgccg tcctcgatca ccttgcccat
     1921 gtagcggaag ggcagcgccg gggcttccgg cttcgcctgc accgccacct gctcgccctg
     1981 cggcgcgccg aagctgcgct gcgcgaaggg atcgctcgcc ggggcctccg cttcacgctt
     2041 ctcgaggcgc gaaacatcga aatcggtctc ggccgagcgc ttctcgctga gcaggttttc
     2101 catcggtttt tcccgtccga cgacgactcc ggcgagcagc gccagcgtca gcacggcgaa
     2161 ggcgaccttc atccgcatca tgagcgcacc aggtgcaggg tgaggtgcag ctcggcctgc
     2221 agggcgccgt cgttgcggct ctcccgctta aggctcatct ggtcgaccg
//
LOCUS       DASYJA010000589         2320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_88531, whole genome shotgun sequence.
ACCESSION   DASYJA010000589 DASYJA010000000
VERSION     DASYJA010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88531"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VGO02_12880"
     CDS             <1..468
                     /locus_tag="VGO02_12880"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEV7478620.1"
                     /translation="ISLGGYYAPRAAAFEPRYACCIAWGAQWDYEKIWRQRFERLARS
                     DTPALSVASQHILWVVNATSQEEALKRLEPFKLDGVVQKIACPFVMLHGEGDEQIPLA
                     EAQKCFDAVGSKQKTMKVFTREEGGYHHCQIDNQSICSAYMWDWLEQVLQPAR"
     gene            complement(684..1661)
                     /locus_tag="VGO02_12885"
     CDS             complement(684..1661)
                     /locus_tag="VGO02_12885"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEV7478621.1"
                     /translation="MRRVVSVAAAVLGLLAWALPGLAQQYPSRVVTLIVPYPAGGPTD
                     QLARVLAPVLSEKLGQNFIVENVSGGGTTIATARVARATPDGHMLLLHNLQISANVAL
                     YNSLPFDTEKDLAPIIFINNNPLVLVGRKTLEPTTLKELFAYMKTKVVKMAHPGVGAT
                     GHLATSLLAQEAKVTVDHIPYRGAAPALQDIAGGHVDLFFATPQSVVEQVASGQMKAY
                     GITAKEMSPQFPNAASFVQELGPKLEILYWHALFAPAGTPDAIVDKLNSALQEIMADP
                     AVVKSWADTGVVPYPKDQRSPAAARALLKSEIARWGQVVRDNNIQAQMQ"
     gene            complement(1876..>2320)
                     /locus_tag="VGO02_12890"
     CDS             complement(1876..>2320)
                     /locus_tag="VGO02_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase C-terminal
                     non-catalytic domain-containing protein"
                     /protein_id="HEV7478622.1"
                     /translation="VLRVFDPAGKEVVFVGSNDPRVPVGLGWLRASHRKLDPQHTRPY
                     RPWHSHDEEWPLTPGEPVELDIEIWPTSIVVPPGYHVALTVRGKDYEYDGTDAGLANA
                     PYPMKGVGPFTHTDPDDRPAAVFACRNTLHFAAGKMPYLLLPVIP"
BASE COUNT          424 a          725 c          775 g          396 t
ORIGIN      
        1 atcagcctcg gcggctacta tgcgccgcgc gccgccgcct tcgagccgcg ctacgcctgc
       61 tgcatcgcct ggggcgcgca gtgggactac gagaaaatct ggcgccagcg cttcgagcgc
      121 ctggcgcgct ccgacacccc ggcgctgtcg gtcgcctcgc agcacattct gtgggtggtc
      181 aatgcaacct cccaggagga ggcgctcaag cggctcgagc cgttcaagct cgatggcgtg
      241 gtgcagaaga tcgcctgccc gttcgtgatg ctgcatggcg aaggagacga acagattccc
      301 ctggccgaag cacagaagtg cttcgacgcg gtcggctcga aacagaaaac gatgaaggtg
      361 ttcacgcgcg aggaaggcgg ctaccaccac tgccagatcg acaaccagag tatctgttcc
      421 gcctatatgt gggactggct cgagcaggtg ctgcagccgg cgcggtgacg tgcggcggag
      481 gcattttacc gccgagttgc cctccgggtg tcatgcgcgg gcttgacccg cgcatccatc
      541 taaaaacgcc tcatgaagag gatggattgc accggaaccc gggcttgtcc gagttccggt
      601 ggataacgcg ctgcaagtcg gataaacccg acttgcagtg tcaagcccgg caatgacggc
      661 ggttgaggca gttccggcct ccgctactgc atctgcgcct ggatattgtt gtcgcgcacg
      721 acctgtcccc agcgggcgat ctcgcttttc agcagcgcac gcgcggccgc aggcgagcgc
      781 tgatccttcg ggtaaggcac gacgccggtg tccgcccagg atttcacgac cgccggatcg
      841 gccatgatct cctgcagggc ggaattgagc ttatcgacga tggcatcagg cgttcccgcc
      901 ggcgcgaaca gcgcgtgcca gtagagaatc tcgagcttcg ggccaagctc ctgcacaaag
      961 ctggccgcgt tcgggaactg cggcgacatc tccttcgcgg tgatgccata ggccttcatc
     1021 tgcccggacg cgacctgctc caccaccgac tgcggcgtgg cgaagaacaa atcgacatgg
     1081 ccgccggcaa tatcctgcag cgccggcgcg gcgccgcgat acgggatgtg atcgacggtg
     1141 actttcgcct cctgcgccag cagcgatgtg gcgagatggc cagtggcgcc gacgccggga
     1201 tgcgccatct tgaccacctt ggtcttcatg taggcgaaca gctccttgag cgtggtgggc
     1261 tccagcgtct tgcggccgac cagcaccagc ggattgttgt tgatgaagat gatgggtgcg
     1321 aggtccttct cggtgtcgaa cggcaggctg ttgtagagcg cgacattcgc cgagatctgc
     1381 agattgtgca ggagcagcat gtggccgtcc ggcgtggcgc gggcgacgcg ggcggtggcg
     1441 atggtggtgc cgccgccgct cacgttctcg acgatgaagt tctgcccgag cttctccgac
     1501 agcaccggcg ccagcacgcg cgcgagctgg tcggtcggac cgccggcggg atagggcacg
     1561 atcagggtga cgacgcggct cggatattgc tgcgccaggc cgggcagcgc ccaggcgaga
     1621 agaccgagaa cagccgctgc aacactgacc acacgccgca tcgaacgctc ctcctgttgt
     1681 agttttgagg gagcttacga cagaagcgtt ttccggcgaa gtgggaaccg gttccggcgc
     1741 gcgttcagca aaagtgggga ccggttttgc gtccgaacgc gcgccaaacg aatgaaatgc
     1801 gcgctttccg ggcggctaac cggtatccac ttagcctgaa agcgcgccag aaaacgcgtc
     1861 aaacgtaagg aatccttacg ggatgaccgg caacaagaga taaggcatct tgccggcggc
     1921 aaagtgcagg gtgttccggc acgcgaaaac agccgcgggg cgatcgtccg gatcggtgtg
     1981 cgtgaacggg ccgacgccct tcatcggata gggcgcattg gcgagccccg cgtcggtgcc
     2041 gtcatattca taatccttgc cgcgcacggt gagcgcgacg tgatagccgg gcggcaccac
     2101 gatcgaggtc ggccagatct cgatgtcgag ctcgaccggc tctcccggcg tcagcggcca
     2161 ttcctcgtcg tggctgtgcc agggccggta cggccgcgtg tgctggggat cgagcttgcg
     2221 atgggaggcg cgcagccagc ccagccccac cggcacgcgc ggatcgttgg agccgacgaa
     2281 caccacctcc ttgccggcgg gatcgaacac ccgcagcacc
//
LOCUS       DASYJA010000590         2399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1813762, whole genome shotgun sequence.
ACCESSION   DASYJA010000590 DASYJA010000000
VERSION     DASYJA010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1813762"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VGO02_12895"
     CDS             <1..918
                     /locus_tag="VGO02_12895"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEV7478623.1"
                     /translation="IVNGQLVAAPVTGMPQVTVHGQGGFFDAVPHPQFEKNQLVYLSY
                     AARGDDGVGTELARARLAGNRLEDVQVLFRQSPKGRSGNHFGGRIVFDRAGYLYLTLG
                     DRGEMPRAQKPDDHAGSVIRLHDDGRVPADNPFVGKPGWKPEKLDLGHRNQQGAALHP
                     QSGVLWTHEHGPQGGDEINLIRPGVNYGWPVITYGVNYGSGSKIGEGTHKDGMAQPIY
                     YWVPSIAPSGMAFYTGERFPKWKGDLFVGSLKFQLLVRLRLDGEKVLQEERLLQGALG
                     RIRDVRMGPDGFLYVVTDENPGVVARLEP"
     gene            918..1712
                     /locus_tag="VGO02_12900"
     CDS             918..1712
                     /locus_tag="VGO02_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEV7478624.1"
                     /translation="MARLSKDERNFYARQGYVVPAWRFPKKKIDEMRGALDRLIADNP
                     GVRPEKLVSAHLENGKEGVKGSRDFLELAREPELVELIADAIGGDVILWGCQVFCKPG
                     GDGLETPFHQDGHYWPIRPLATCTAWVALDPSTRQNGCLKVVPGSHSEKKLFSHLTED
                     RQDLVLTQRIQDPGFDEAKAVAIELEPGQMSLHDIYMIHGATVNRSPQRRAGVAIRYM
                     PGSSVFLRDLIKPNSSSGYLVDFASRPLWLLKGKDRTGKNDFSIGH"
     gene            complement(1737..>2399)
                     /locus_tag="VGO02_12905"
     CDS             complement(1737..>2399)
                     /locus_tag="VGO02_12905"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7478625.1"
                     /translation="TRHAATMDLRYGLAERNRIDLFPAGPAAAPLLVFIHGGYWQMRA
                     KEVFAFVAAGPLAHGISVALAGYTLAPQIRLDGIVAEIRSCLDYLSKEHSHIVLSGWS
                     AGGHLTAMCMSHPAVKAGLAISGIYDLEPMRLSYINDKLRLDEAEQKRNSPIFLSNKK
                     KLTVAYGGAELPELQRQSEDYAQAAKAKILPLAGRDHFTILEELASPQGALTTALRTL
                     IE"
BASE COUNT          437 a          756 c          821 g          385 t
ORIGIN      
        1 attgtgaatg gccagctggt tgccgcgccg gtcaccggca tgccgcaggt gacggtgcac
       61 ggccagggcg gcttcttcga cgccgtgccg catccgcagt tcgagaagaa ccagctcgtg
      121 tacctctcgt atgcagcgcg cggcgacgac ggggtaggga ccgagctcgc gcgcgcgaga
      181 ctggcgggga accggctgga ggacgtccag gtcctgttcc gccagagccc caagggccgc
      241 agcggaaatc acttcggcgg gcgcatcgtg ttcgaccgcg ccggctacct ctacctgacg
      301 ctgggcgatc gcggcgagat gccgcgcgcg cagaagcccg acgaccacgc cggctcggtg
      361 atccggctgc acgacgacgg ccgcgtgccg gcggacaatc cgttcgtcgg caagccgggc
      421 tggaagccgg agaagctcga cctggggcac cgcaaccagc agggcgcggc gctgcatccg
      481 cagagcggcg tgctgtggac gcacgagcac ggtccgcagg gcggcgacga gatcaacctc
      541 atccgtcccg gcgtcaacta cggctggccg gtcatcacct acggcgtgaa ctacggcagc
      601 ggcagcaaga tcggcgaggg cacgcacaag gacggcatgg cgcagccgat ctactactgg
      661 gtgccgtcca tcgcgccttc cggcatggcg ttctacaccg gcgagcgctt cccgaagtgg
      721 aaaggcgacc tgttcgtcgg ctcgctcaag ttccagctgc tggtgcgcct gcgcctcgac
      781 ggcgaaaaag tgctgcagga ggagcgcctg ctgcagggcg cgcttggccg catccgcgac
      841 gtgcgcatgg gacccgacgg ttttctctac gtggtcaccg atgaaaatcc cggcgtggtt
      901 gcgcgcctgg agccgtgatg gccagattga gcaaagacga aaggaacttc tatgccaggc
      961 agggctatgt agtccctgcc tggcgattcc ccaagaagaa aatcgacgag atgcgcgggg
     1021 cgctcgaccg actcatcgcc gacaacccgg gcgtgcgccc ggagaagctg gtcagcgcgc
     1081 acctcgagaa cggcaaggag ggcgtgaagg gcagccgcga cttcctcgag ctggcgcgcg
     1141 agccggagct ggtcgagctc atcgccgacg ccatcggcgg cgacgtcatc ctctggggct
     1201 gccaggtgtt ctgcaagccc ggcggcgacg gcctggagac gccgttccac caggacgggc
     1261 actactggcc gatccgcccg ctcgccacct gcaccgcgtg ggtcgccctc gacccttcca
     1321 cgaggcagaa cggctgcctg aaggtggtgc ccggttctca ttcggaaaag aagctgttct
     1381 cccatttgac ggaagacagg caggacctgg tcctcacgca acgcatccag gacccgggat
     1441 tcgatgaagc aaaggcggtg gcaatcgagc tcgagcccgg acagatgtcg ctgcacgaca
     1501 tctacatgat ccacggcgcc accgtgaacc gctcgccgca gcgtcgcgcc ggcgtcgcga
     1561 tccgctatat gccaggcagt tccgtttttc tcagagatct aatcaagcca aattcgagca
     1621 gcggctacct ggtcgatttc gcgagccggc cgctgtggct gctcaaggga aaagaccgga
     1681 ccggaaagaa cgacttctcc atcggacatt gagaaataaa tggggtcaga ccccatttat
     1741 tcgataaggg tgcgcagcgc cgtcgtcagg gccccttggg gcgaagcgag ctcttcgagg
     1801 atcgtgaagt gatcgcggcc ggcgagggga aggatttttg ccttggccgc ctgggcatag
     1861 tcttcggact gcctctgcag ctccggcagc tcggcccctc cgtacgcgac ggtcaatttc
     1921 ttcttgtttg aaagaaaaat cgggctgttg cgcttctgct cggcctcgtc gagccgcagc
     1981 ttgtcgttga tgtacgaaag ccgcatcggc tcgaggtcgt agatgccgct gatggcgagt
     2041 cccgccttta cggccgggtg ggacatgcac atcgcggtga ggtggccgcc ggccgaccag
     2101 cccgaaagga cgatgtggga atgctctttc gaaaggtagt cgaggcagga gcggatctcc
     2161 gccacgatgc cgtccagcct gatctgcggg gcgagggtat agcccgccag cgcgacgctg
     2221 atgccgtggg ccaggggacc ggccgcgacg aaggcgaaca cctccttcgc gcgcatctgc
     2281 cagtagccgc cgtggatgaa aaccagcaag ggggctgcgg ccggcccggc ggggaacagg
     2341 tcgatgcggt tgcgctcggc gaggccgtag cgcaggtcca tggtggcggc atgccgggt
//
LOCUS       DASYJA010000591         2564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2177718, whole genome shotgun sequence.
ACCESSION   DASYJA010000591 DASYJA010000000
VERSION     DASYJA010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2177718"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VGO02_12910"
     CDS             <1..465
                     /locus_tag="VGO02_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV7478626.1"
                     /translation="ELDEASFDRVVAVDLKGVFLCLTYQLRHMSAGAIVNTASVAGLV
                     PEAGSGAYVAAKHGVIGLTKTAALENAHRGIRVNAIAPGWVRTPMTRRWDEDQEFNAK
                     LKAATPMHRGAEPEEVAGVVLFLCSPAASYLTGQVLTVDGAQTIRGLLPPRY"
     gene            465..1235
                     /locus_tag="VGO02_12915"
     CDS             465..1235
                     /locus_tag="VGO02_12915"
                     /inference="COORDINATES: protein motif:HMM:NF007341.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEV7478627.1"
                     /translation="MERKYRLVQGLVRGLEVLRAMNQAAGASGSSTTLAQATGLHRTT
                     VKRLLETLREAGFVRRLPDDSFRLTFRVRTLSDGFTDETWVAQVAAPLLRQLTEQVRW
                     PSDLVTLEGEELVIRDTTHTFSPLSFHPAMMGQRLPFLQTAVGRAYLAFCAAEERDAL
                     LAMLRDRSDKDGQLARDTRRVRTMLQSTRARGYAINEGDWIGGGRFGALAVPIMHHGH
                     ARACINLIFSKRAISTAQAAAKYLASMKKTAEAIEANL"
     gene            complement(1242..1317)
                     /locus_tag="VGO02_12920"
     tRNA            complement(1242..1317)
                     /locus_tag="VGO02_12920"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1281..1283),aa:Glu,seq:ttc)
     gene            complement(1336..1411)
                     /locus_tag="VGO02_12925"
     tRNA            complement(1336..1411)
                     /locus_tag="VGO02_12925"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1376..1378),aa:Ala,seq:ggc)
     gene            complement(1452..>2564)
                     /gene="gltX"
                     /locus_tag="VGO02_12930"
     CDS             complement(1452..>2564)
                     /gene="gltX"
                     /locus_tag="VGO02_12930"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV7478628.1"
                     /translation="GHAYEDYMSREELEALRKEQLSKNIKPKYDGRWRPENVKRLNLK
                     KPAGVKPVIRFRTPEEGSVGWQDLVKGLIEFPNAELDDLVLLRADGVPTYNFGVVVDD
                     LDMDITHVIRGDDHVNNTPRQIHIYRSLRAAIPEFAHVPMILGADGERLSKRHGATSV
                     IDYREQGYLPEALLNYLARLGWSHGDSEVFSKNDLIEWFDLAHVSHSSAQFNPEKLAW
                     LNQQHIKTADDRRLAGLLKDEFAKRGVDASAGPPLERVVALVKDRANSILLLADEAML
                     FYAGEIPTAQIPADAVNALSRLRAKLESVAWERPAISEAIKQVLKESSLKMPQLAMPL
                     RQAVTGRTQTPSIDAVLELLGRETVLARLSAQLSRG"
BASE COUNT          422 a          830 c          843 g          469 t
ORIGIN      
        1 gagctcgacg aggcgagctt cgaccgcgtc gtggcggtcg acctgaaggg cgtgttcctg
       61 tgtctcacgt accagcttcg gcacatgagc gcgggagcta tcgtgaacac cgcatccgtc
      121 gccggcctgg tgcccgaagc cggctcaggt gcctacgtcg ctgccaagca cggggtcatc
      181 ggcctgacga aaaccgccgc gctggagaac gcgcatcgcg gcatccgcgt caatgcgatt
      241 gcgccgggtt gggtgcgcac gccgatgacg cgccgctggg acgaagatca ggagttcaac
      301 gcgaagctga aagcggcgac gccgatgcat cgcggcgcag agcccgagga ggttgcgggc
      361 gtggtgcttt ttctctgctc gcccgcggcc tcgtacttga ccggccaggt cctgaccgta
      421 gacggggcgc agacgatccg cggtctcctg ccgccgagat actgatggag cggaagtatc
      481 gccttgtcca gggcctggtg cgcgggctcg aggtgctgcg ggcgatgaat caggcggccg
      541 gcgcgagcgg atcctcgacg acgctcgccc aggcgaccgg gctgcatcgc acgacggtga
      601 agcgcctgct cgagacgttg cgcgaggcgg gcttcgtgcg gcgcctgccc gacgacagct
      661 tccgcctcac cttccgggta cgcaccttga gcgacggatt caccgacgag acgtgggtcg
      721 cgcaggtcgc ggcgccgctc ctgcggcagc tcaccgaaca ggtccgttgg ccaagcgacc
      781 tcgtcacgct ggaaggcgaa gagctggtga tccgggacac cacgcacacc ttcagcccgc
      841 tctccttcca tcctgccatg atgggtcagc gcctgccctt cctgcagacc gcggtcggac
      901 gagcctatct cgccttctgc gctgcggagg aacgcgatgc gcttctcgcc atgctgcggg
      961 accgcagcga caaggatggg cagctggcac gcgacacacg gcgcgtacgg acaatgctgc
     1021 agtcgacgcg cgcgcgtggc tacgccatca acgaaggcga ctggatcggg ggcgggcgct
     1081 tcggcgcgct ggcggtgccg atcatgcacc acgggcacgc gcgagcctgc atcaacttga
     1141 tcttttcaaa gcgcgcgatt tccactgcgc aagcggccgc gaaatacctc gcgagcatga
     1201 aaaagaccgc cgaagccatc gaagcaaacc tctagcaggt atggcgtccc cacggggatt
     1261 cgaaccccgg ttaccgccgt gaaagggcga tgtcctaggc ctctagacga tagggactgc
     1321 gtacagcaga tcggttggtg gaggtaagcg ggatcgaacc gctgacctct tgcatgccat
     1381 gcaagcgctc tcccagctga gctatacccc cgaccgaggg ggcggattat agcgcctccc
     1441 ggcgcgggaa atcaccccct gctcagttgc gccgaaaggc gcgcgaggac cgtttcgcgg
     1501 ccgagcaatt cgagcaccgc gtcgatcgac ggcgtctgcg tgcggccggt gaccgcctgg
     1561 cgcaggggca tggcgagctg cggcattttc aggctgcttt ccttgagcac ctgcttgatc
     1621 gcctcgctga tcgccgggcg ctcccaggcc acgctttcga gtttcgctct cagcctggac
     1681 agggcattca cggcgtccgc gggtatttgc gcggtgggga tttcccctgc atagaagagc
     1741 atcgcctcgt cggcgagcag caggatcgag ttcgcgcggt ccttgaccag cgccaccacg
     1801 cgctcgagcg gcgggccggc gcttgcgtcc acgccccgct tggcaaattc gtccttgaga
     1861 agaccggcca ggcgccggtc gtccgccgtc ttgatgtgct gctggttcag ccaggcgagc
     1921 ttctcggggt tgaactgcgc cgaggagtgc gagacgtggg ccaagtcaaa ccattctatt
     1981 aaatcatttt ttgaaaaaac ctcgctgtcg ccatgcgacc agcccaggcg cgcgaggtag
     2041 ttgagcagcg cttccggcag gtagccctgc tcgcggtagt cgatgacgct ggtcgcgccg
     2101 tggcgcttcg acagccgctc gccgtcggcg ccgaggatca tcggcacgtg ggcgaattcc
     2161 gggatcgccg ctctcaggct tctatatatg tgtatctgcc gcggcgtgtt gttgacgtgg
     2221 tcgtcgccgc ggatgacgtg cgtgatgtcc atgtccaggt cgtcgaccac cacgccgaag
     2281 ttgtaggtcg gcacgccgtc ggcgcgcagc agcaccaggt cgtcgagctc ggcgttgggg
     2341 aactcgatca ggcccttcac caggtcctgc cagccgacgc tgccctcctc cggcgtgcgg
     2401 aagcggatca ccggcttcac gcccgccggc tttttcaaat tcaatctctt tacgttctcg
     2461 gggcgccagc ggccgtcata ctttggtttg atgttttttg aaagctgctc ttttcgcagc
     2521 gcttccagct cttcgcgcga catgtagtct tcataggcat gccc
//
LOCUS       DASYJA010000592         2472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1541704, whole genome shotgun sequence.
ACCESSION   DASYJA010000592 DASYJA010000000
VERSION     DASYJA010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541704"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..619)
                     /locus_tag="VGO02_12935"
     CDS             complement(89..619)
                     /locus_tag="VGO02_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478629.1"
                     /translation="MIVVLLSLAGCAYQSDFSIAPNLKDIDTSRGPAPTYREAITAAP
                     VSGGKEDDLFSVPNISNKAFQAALVRTLLNAKLASASNGRYRLDATLKLDQPFITFNT
                     TVTAIVAYRLTDVATGTVVYDKTLETQGTAEYMEIAVASERMKLANSRAVRANLRRLV
                     EELYALPDPPGSAPRS"
     gene            827..1059
                     /locus_tag="VGO02_12940"
                     /pseudo
     CDS             827..1059
                     /locus_tag="VGO02_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012493816.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
     gene            complement(1037..1582)
                     /locus_tag="VGO02_12945"
     CDS             complement(1037..1582)
                     /locus_tag="VGO02_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478630.1"
                     /translation="MAMLLLGALGACAPPPSYTSMVPELALAELGPGPAPSYRGAITV
                     APVSISTDTSTPWTSQVGSADFQEALLRTLTFANLANQQNGRFRLDAVLLRLERPYAG
                     FAMTVTASVAYRLTEIATGAVVYQETRTSLGSASLNDAVTNTNRLRIADERALRANIR
                     KLVEDLYALPERASPISSRRT"
     gene            complement(1623..>2472)
                     /locus_tag="VGO02_12950"
     CDS             complement(1623..>2472)
                     /locus_tag="VGO02_12950"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV7478631.1"
                     /translation="QLTAVSAAGFRCVAPDMRGYGGTEAPEPIDQYTLHHLVGDMAEL
                     VKALSEKDGGESRATIVGHDWGAPVAWHAALWRPDLFPAVCAMSVPYAPPGYVDILSA
                     LEKLGINDFYLQYFQKPGVPEAELQQDIRGALRRLYFTAGGDLQEKGKGFARLTDGTL
                     LGNTVDPPTLPAWLSEADLDYYTQEFARTGFRGGLNWYRNLRRNWELGGPWRGQPIRQ
                     PSLFIAGSRDGVLRFPAAQSQLDAFPKTLPGLRGSHIIEGAGHWVQQEAPAEVNKLLI
                     DFLKGL"
BASE COUNT          442 a          774 c          863 g          393 t
ORIGIN      
        1 cttgcggcgg ccggcgcgct cgacctcgcc atgctgtcgg tcgccaccgc cgagttgcgc
       61 accctggtct agcgatcgaa agcggcgctc aacttcgcgg cgcggaaccg ggcggatccg
      121 gcagagcgta cagttcctcg acgaggcgac gcaggtttgc ccgcacggct cgcgagttgg
      181 cgagcttcat tcgttcggag gcgaccgcta tttccatgta ttccgcggtg ccttgcgtct
      241 ccagcgtctt gtcgtagacc accgtgcccg tcgccacgtc ggttaaacgg taggcgacaa
      301 tggccgtcac cgtcgtgttg aaggtgatga atggctggtc gagcttgagc gtcgcgtcaa
      361 ggcggtatcg tccattgctc gcactcgcca gtttggcgtt caacagcgtg cgcaccaagg
      421 ccgcctgaaa ggccttgttg ctgatatttg gcaccgagaa gagatcgtct tccttgccgc
      481 cactgacagg ggcagccgtt atggcctcac gataggtcgg cgcgggaccg cgcgaagtgt
      541 cgatatcctt cagattggga gcgatggaaa agtcggactg gtaggcgcag ccggcaagcg
      601 acagcagcac gacgatcatc agcatgccga atgaacgcat gacaccgacc ggattcctgg
      661 tgaatgaggt agacagagcc cacacgctgc aaagtatgtc gcatgcgaag cgggccggct
      721 agaccggata gcgagcaccc aagcggagtc gcaccgtgct cgccgagaag gaccaatctt
      781 cgtcactgga gacgccatga gcaccaagac ctatcccgtc cagaagagcg aggccgagtg
      841 gcagaaggag ctcgatccgc agcagtacaa ggtgctgcgc aagcacgcca cggagcgcgc
      901 cggcaggagc ccgctcaacc acgagaagcg caagggcgag aggtacactg cgcccgctgc
      961 ggcggccatc tcggccacgt ctttcccgat gggccgaagc ccaccggcca gcgctattgc
     1021 atgaacggcg cggcgctcaa gttcgacgag aagaaatagg cgaggcgcgc tcgggcagcg
     1081 cgtagagatc ctcgaccagc ttgcggatat tggcgcgcag cgcccgctcg tcggcgatgc
     1141 gcagccggtt ggtgttggtg acggcgtcgt tcaggctggc gctgccgagc gaggtgcgcg
     1201 tctcctgata gacgacggcg ccggtcgcga tctcggtcag ccgataggcg accgacgcgg
     1261 tgacggtcat ggcgaagccg gcatagggtc gctcaagccg gagcaggacg gcgtcgaggc
     1321 gaaagcggcc gttctgctga ttcgccaggt tggcgaacgt caacgtccgc agcagcgctt
     1381 cctggaagtc ggcgctgccc acctgcgacg tccagggcgt gctggtgtcg gtgctaatgc
     1441 tgacgggcgc gacagtgatc gcgccgcgat aggacggcgc ggggccggga cccagctcgg
     1501 cgagcgcaag ttcgggcacc atcgaggtat agctgggcgg cggcgcgcag gcgccaagcg
     1561 cgccgagcag gagcatcgcc acgagaagac ggatcatcgg cgcctccggt atgccaagcc
     1621 catcacaggc ccttcaggaa gtcgattaac agtttgttga cctcggccgg cgcttcctgc
     1681 tgcacccagt ggccggcgcc ctcgatgatg tggctgccac gcaggccggg gagagtcttg
     1741 gggaaggcgt cgagctgcga ctgcgcggcg ggaaagcgca gcacgccgtc gcggctgccg
     1801 gcgatgaaca aggacggctg gcggatcggc tggccgcgcc acggcccgcc gagctcccag
     1861 ttgcgccgca ggttgcgata ccagttgagc ccgccgcgaa agccggtgcg cgcgaattcc
     1921 tgcgtgtaat agtcgaggtc ggcctcgctc agccaagcgg gcagcgtcgg cggatcgacg
     1981 gtgttgccga gcaaggtgcc gtcggtcagg cgggcgaagc ctttgccctt ttcctgaaga
     2041 tcgccgccgg cggtgaaata gaggcggcgc agggcgccgc ggatatcctg ctgcagctcg
     2101 gcctcgggca cgccgggctt ctggaaatac tgcagataga agtcgttgat gccgagcttc
     2161 tcgagcgcgc tcaggatgtc gacatagccc ggcggcgcgt agggcacgct catggcgcag
     2221 acggcgggaa acagatcagg gcgccagagg gccgcgtgcc aggcgaccgg cgcgccccaa
     2281 tcgtggccga cgatcgtggc ccggctctcg cccccatctt tttcgctgag ggctttcacc
     2341 agctcggcca tgtcgcccac cagatgatgg agcgtgtact ggtcgatcgg ctcgggcgct
     2401 tcggtgccgc cgtagccccg catgtcgggc gccacgcagc gaaagcccgc cgcgctcacg
     2461 gccgtgagct ga
//
LOCUS       DASYJA010000593         2969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2541124, whole genome shotgun sequence.
ACCESSION   DASYJA010000593 DASYJA010000000
VERSION     DASYJA010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2541124"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..1465)
                     /gene="cysS"
                     /locus_tag="VGO02_12955"
     CDS             complement(119..1465)
                     /gene="cysS"
                     /locus_tag="VGO02_12955"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV7478632.1"
                     /translation="MKIYNSLGRTKQEFTPLHSGEVRMYVCGMTVYDYCHLGHARVLV
                     VFDVVRRWLRASGYRVNYVRNITDIDDKIIRRARENGEPVSALTERFIRYMDEDAAAL
                     GVQTPDHEPRATQYVPQMLDLIGTLERKKLAYQSGSGDVNYAVRRFPGYGKLSGKSLD
                     ELRAGERVEVDDSKEDPLDFVLWKRSKPEEPSWPSRWGAGRPGWHIECSAMSGSLLGE
                     TFDIHGGGQDLQFPHHENEIAQSEGASGHPFVKYWMHNGFVRVDEEKMSKSLGNFFTV
                     REVLKKFDAEVVRFFILRAHYRSPLNYADAHLDDAKSALGRLYTALKNSGENAPVDWS
                     EAHAVRFKAAMDDDFNTPEAVAALFDLAGRVNAGDKRLAPQLRALGGVLGILQRSPEA
                     FFQGGLDATAIEAMIRAREAARKGRDFAEADRIRKDLLAKGIVLEDGPGGTTWRRA"
     gene            1578..2540
                     /locus_tag="VGO02_12960"
     CDS             1578..2540
                     /locus_tag="VGO02_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV7478633.1"
                     /translation="MVSLSACCLLAFAARADDLQDASKLLKSGQHQQALERVNKVLAA
                     KPRDPQARFLKGLIFTDQGNTKDAIEIFTKLTQDYPELPEPYNNLAVIYAAQGQYDKA
                     RVALEQSIRTHPSYATAYENLGDVYAKLASQAYDKALQIDSSNKGAKNKLSLVRDLVG
                     GSTPAATVAIAKAPEKPVAEKPAPEKPAVAEKPAEKAVEKPAAKPAADGSAEVLAAVN
                     AWAQAWSKRDADGYLSYYAADFKAPGKESRADWEKGRRTRLSAAKTIKVGIDSPKVTM
                     SGSNSATVVFQQNYQSDTFKSAGRKTLVMAKSGERWQIREEKSN"
     gene            2540..>2969
                     /locus_tag="VGO02_12965"
     CDS             2540..>2969
                     /locus_tag="VGO02_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478634.1"
                     /translation="MRGLLLALLLACPAFAPAAAPAPTPEDRLSAVFESIEANRLDDA
                     MQRVDALIRDYPNFRLAHLVRGDLLLARSRPLLTFGNVVKTVPQEKIDGMREEALARL
                     RAHRQRPAEDRLPRLVLQLHPAQKHLLLVDSLRSRLYVFAN"
BASE COUNT          527 a          996 c          952 g          494 t
ORIGIN      
        1 gaaatcctcc aggaaggagg cgaaaaacct taggaattaa gaactcgtac gactttcttg
       61 ataagcacta tcaacttctc ccgatgcact ggcaagcttt tccagccgga tcagaggctt
      121 aggcgcgacg ccaggtggtg ccgccgggac cgtcctccag gacgatgcct ttggccagca
      181 agtccttgcg gatgcggtcg gcctcggcga aatcccggcc cttcctcgcc gcctcgcgcg
      241 cgcggatcat cgcctcgatg gcggtcgcat ccaggccgcc ctggaagaac gcctccgggc
      301 tgcgctgcag gatgccgagg acgccgccca gggcgcgcag ttgcggcgcc aggcgcttgt
      361 cgccggcgtt cacccggccg gcgagatcga acagcgcggc gaccgcctcc ggcgtattga
      421 agtcgtcgtc catcgccgcc ttgaagcgca cggcgtgcgc ctcgctccag tcgaccggcg
      481 cgttctcgcc ggaattcttc aacgccgtgt acaggcgccc gagcgcgctt ttcgcgtcgt
      541 cgaggtgggc gtccgcatag ttgagcgggc tgcggtagtg cgcgcgcagg atgaagaagc
      601 gcaccacttc ggcgtcgaac ttcttcagca cctcgcgcac ggtgaagaag ttgccgagcg
      661 acttcgacat cttctcctcg tcgacgcgca cgaagccgtt gtgcatccag tacttgacga
      721 agggatggcc gctcgcgcct tcggactggg cgatctcgtt ctcgtggtga gggaactgca
      781 ggtcctgtcc gccgccgtgg atatcgaagg tctcgccgag gaggctgccg ctcatggccg
      841 agcactcgat gtgccatccg gggcgccccg ctccccagcg cgacggccag ctcggctcct
      901 ccggcttgga gcgcttccac agcacgaaat ccagcgggtc ctccttggaa tcgtcgacct
      961 cgacccgctc tccggcgcgc agctcgtcga gcgatttccc ggaaagcttg ccgtacccgg
     1021 gaaagcggcg caccgcgtag ttcacgtcgc cggaacccga ctggtaggcc agcttcttgc
     1081 gctcgagcgt gccgatcagg tccagcatct gcggcacgta ttgcgtggcg cgcggctcgt
     1141 gatcgggcgt ctgcacgccg agcgcggcgg cgtcctcgtc catgtagcgg atgaagcgct
     1201 cggtcagcgc gcttaccggc tcgccgtttt cccgcgcgcg ccggatgatc ttgtcgtcga
     1261 tgtccgtgat attccgcacg tagttgaccc ggtagccgct cgcccgcagc cagcgtcgca
     1321 cgacgtcgaa gaccacgagg acgcgggcgt ggccgagatg gcagtagtcg tacacggtca
     1381 tgccgcacac gtacatgcgc acttcgcccg aatgcagggg ggtgaactcc tgcttggtcc
     1441 tgccgaggct gttgtagatc ttcagaaagg ttgtcgcgcc gcgacgctta gtctagaatg
     1501 cgaaatctta cgtagggcct tgaaaatata acacaatgct gatttcagcc acccgcttgg
     1561 caaagctagg gcggggtgtg gtttccctgt ccgcctgctg cctcctcgct tttgccgcac
     1621 gcgccgacga tctgcaggat gcgagcaagc tgctgaagtc cgggcagcat cagcaggcgc
     1681 tcgagcgcgt aaacaaggtg ctcgccgcga aaccgcgcga tccgcaggcg cgctttctga
     1741 agggactgat cttcaccgat cagggcaaca ccaaggacgc gatcgagatc ttcaccaagc
     1801 tcacgcagga ttatccggag ctgcccgagc cctacaacaa tctcgccgtg atctatgcgg
     1861 cgcagggcca gtacgacaag gcgcgcgtcg cgctcgagca atccatccgc acgcacccga
     1921 gctacgccac cgcctacgag aacctcggcg acgtgtacgc gaagctcgcc agccaggcct
     1981 acgacaaggc gctgcagatc gattcgagca acaagggcgc gaagaacaag ctctcgctgg
     2041 tgcgcgacct ggtgggcgga tccacgccgg ccgcaacggt cgccatcgcc aaggcgccgg
     2101 agaagccggt ggcggagaag cccgcgccgg aaaagccggc cgtcgcagag aaaccggcgg
     2161 agaaggctgt ggagaagcct gccgccaagc ctgccgccga cggctcggcc gaggtactgg
     2221 cggcggtcaa cgcgtgggcg caagcctggt ccaagcggga cgccgatggc tacctctcgt
     2281 attacgccgc cgacttcaag gcgccgggca aggagtcgcg tgccgactgg gaaaaaggac
     2341 gccgcacgcg cctcagcgcg gcgaaaacga tcaaggtcgg catagactcc cccaaagtaa
     2401 ccatgtcggg cagtaacagc gccaccgtgg tcttccagca gaattaccag tccgacacct
     2461 tcaagagcgc gggcaggaag accctggtga tggccaagtc cggcgagcgc tggcaaatcc
     2521 gcgaggaaaa gagcaactga tgcgcggctt gctcctcgcg ctcctgctgg cctgcccggc
     2581 gttcgcgccg gcggccgctc cggcgcccac gcccgaggac cggctctcgg ctgtcttcga
     2641 gtcgatcgaa gccaaccggc tcgacgacgc gatgcagcgc gtcgacgcgc tgatccgcga
     2701 ctatccgaat ttccgcctgg cgcatctggt gcgcggcgat ctgctgctgg cgcgctcgcg
     2761 tcccctgctg accttcggca acgtagtcaa gaccgtgccg caggaaaaga tcgatggcat
     2821 gcgcgaggag gcgctggcgc gcctgcgggc ccaccggcag cgccccgccg aggaccgcct
     2881 gccgcggctc gtgctgcagc tgcatccggc gcagaagcat ctcctgctgg tcgattcgct
     2941 tcgctcgcgc ctctatgtgt tcgccaacg
//
LOCUS       DASYJA010000594         4025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1996885, whole genome shotgun sequence.
ACCESSION   DASYJA010000594 DASYJA010000000
VERSION     DASYJA010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1996885"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VGO02_12970"
     CDS             complement(<1..881)
                     /locus_tag="VGO02_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein/thiosulfate
                     sulfurtransferase GlpE"
                     /protein_id="HEV7478635.1"
                     /translation="MFANVLLEQIGLPIPAVPTLVLAGALSADGRLSVGAVLGVALLA
                     CTLGDTAWYVAGRRYGNRVLKLLCRVSLSPDSCVRQSETQFERWGRLALVLGKFVPGL
                     STIAPPLAGVMRLRWPEFLVLNGIGILLWAGLAVGAGLLFHAQVDYLVGRLADLGAAA
                     AVLGAALLGGYIALKWWQRRRFYKMLRLARITADDLRGLLRTRNGTVVFDVRSRVARG
                     RDPRFIPGALALDIEELDKRLDELPADEEIVFYCSCPNEASAAAVARRLIDRGYTRVR
                     PLLGGLEAWVDAGYEVEA"
     gene            complement(927..1166)
                     /locus_tag="VGO02_12975"
     CDS             complement(927..1166)
                     /locus_tag="VGO02_12975"
                     /inference="COORDINATES: protein motif:HMM:NF022397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HEV7478636.1"
                     /translation="MKTPDQSVLSHIQSLVAEEHRLFERGELTPEDGKRLKEVQAHLD
                     QCWDLLRQRRALRETGGDPSQAHARPDDVIKKYIA"
     gene            1331..1534
                     /locus_tag="VGO02_12980"
     CDS             1331..1534
                     /locus_tag="VGO02_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEV7478637.1"
                     /translation="MPTGTVKWFNDSKGFGFITPDDGGKDLFAHHSSIQMDGYKSLKE
                     AQKVSFEVKDGPKGPAAANIKPI"
     gene            1618..1812
                     /locus_tag="VGO02_12985"
     CDS             1618..1812
                     /locus_tag="VGO02_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478638.1"
                     /translation="MIASLFMPAAALLAVLCALLFDVSPHAFVTFGGLFNEPLGLLAW
                     WVACLVPASAYAVMCFRNAN"
     gene            complement(1809..2423)
                     /locus_tag="VGO02_12990"
     CDS             complement(1809..2423)
                     /locus_tag="VGO02_12990"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEV7478639.1"
                     /translation="MLTLYYAAGACSLAAHIVLEESGEKYEARKMDLSKGEQRTPEYL
                     KIHPLGRVPALLLDSGKPLTENTAILPYLGKRFKLWPAEPIADAQALSVIGFFAASVH
                     PAHGHIGRPERYAADKAAHASIQETGKKSFVGYLEQIDGMYAGREWLGDKYSVLDPYA
                     LVFYSWGVKRELGVQQLKNYTAAKERMMQRPAVRKIIENEGVKV"
     gene            complement(2436..3212)
                     /locus_tag="VGO02_12995"
     CDS             complement(2436..3212)
                     /locus_tag="VGO02_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HEV7478640.1"
                     /translation="MDDPREVRRSIRSGRHRKHTAGLARGYVQGNVCILPREYAADFR
                     AFCERNPKPCPLLAMSEPGDPRLPSLGEDLDIRTDVSRYRIFHDGVLREEVSNLKQAW
                     RDDLVTFVLGCSFSFEEALLGAGLPLRYVEQGRNVPMYRTSVETVPAGRFRGKLVVSM
                     RPFRPADAIRAIEITSRYPRVHGAPVHIGFPELIGITDLSHPWVGDPTEVRADELPLF
                     WACGVTPQSVVLDAKPSLCITHSPGHMLVTDLKNSSLIEN"
     gene            3242..>4025
                     /locus_tag="VGO02_13000"
     CDS             3242..>4025
                     /locus_tag="VGO02_13000"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV7478641.1"
                     /translation="MKPAYVPAGVIPAAVLPFHEDLSIDEASYRAHLRDLAAVEGISA
                     ITVNAHASEVASCTFDEQRRVLEVTREELGALPIVNGVYADGSLEAARIAKMAAQGGA
                     SALLVFPPGIYTFGQRPEMAIEHFRRIAGATDLPLILFQYPLAGGQGYPTSTLLELVE
                     KVPTVRAIKDWCANPQLHERHIRLLQSRARPVNVLTTHSSWLMSSLVLGCSGLLSGSG
                     SVIADLQVKLFRAVRANDLAGAKKINDRIRPTAEAFYAEPWVD"
BASE COUNT          677 a         1350 c         1347 g          651 t
ORIGIN      
        1 gcttcaacct cgtatccggc gtcgacccag gcctcgagcc cgccgagcag cggccgcacg
       61 cgcgtgtagc cgcggtcgat caggcgccgc gctacggcag ccgcggaggc ttcgttaggg
      121 caggagcagt agaagacgat ctcctcgtcg gccggcagct cgtcgagccg cttgtcgagc
      181 tcctcgatgt cgagcgcgag cgcccccgga atgaagcgcg gatcccggcc gcgcgcgacg
      241 cgcgagcgca cgtcgaagac cacggttccg ttgcgcgtgc gcaggagccc gcgcaggtcg
      301 tccgccgtga tgcgcgcgag gcgcagcatc ttgtagaagc gccgccgctg ccaccacttg
      361 agggcgatgt agccgccgag cagcgccgcg ccgagcacgg cggccgcggc gccgagatcc
      421 gcgaggcgcc cgacgagata gtcgacctgc gcgtggaaca gcaggccggc gcccaccgcc
      481 agcccggccc agagcaggat gccgatgcca ttgaggacga ggaactcggg ccagcgcagg
      541 cgcatgacgc cggccagcgg cggcgcgatg gtcgacaggc cgggaacgaa cttgcccagg
      601 acgagcgcga ggcgccccca gcgctcgaac tgcgtttcgg actggcgcac gcacgagtcg
      661 ggcgaaagcg acacgcggca cagcagcttc aggacgcggt tgccgtagcg gcgtccggcg
      721 acgtaccacg cggtgtcgcc gagggtgcag gcgagcagcg caacgcccag caccgcgccg
      781 accgaaaggc gtccgtcggc ggaaagcgcg cccgccagga ccagcgtcgg gacggcgggg
      841 atgggcaggc cgatctgctc gagcagcacg ttggcgaaca ccagcgcgag gccgtactgc
      901 gcgatgagga gagtgaattc ctgcagctag gcgatgtact tcttgatcac gtcgtccggg
      961 cgcgcgtgcg cctgcgacgg atcgccgccg gtctcgcgca gcgcccggcg ctggcgcagc
     1021 aggtcccagc actggtcgag gtgcgcctgc acctccttga ggcgcttgcc gtcttccggc
     1081 gtgagctcgc cgcgctcgaa gaggcggtgc tcttcggcga ccaggctctg gatatgggaa
     1141 agcacggatt ggtcgggggt tttcatggga atgtgataca gtgcgcgcgc aggatcagtc
     1201 aagtagtgaa gtgcgcagtc cctccctcgc cgcgcaagcg gtgccgaagt ggccgttcaa
     1261 ttccttcctt gatcgtcatg cccgtattcg cggcccggat tcgccgcgta ctcaagaagg
     1321 aagcaccaac atgcctaccg gcacagtgaa atggttcaac gattcaaaag gtttcggctt
     1381 tatcaccccc gatgatggcg gcaaggatct gttcgcccat cacagctcga tccagatgga
     1441 cggctacaag tccctgaagg aggcgcagaa ggtctcgttc gaggtcaagg acggccccaa
     1501 gggaccggca gcagccaaca tcaagccgat ctgagcatca caaaagacat cggaacaaaa
     1561 ggcccgggcg accgggcctt ttactttccg ctcggccagt tcgcgatcct gacggcggtg
     1621 atcgcgagcc tgttcatgcc tgccgcggcg ctcctcgccg tgctctgcgc cctgctcttc
     1681 gacgtatcgc cgcacgcctt cgtgaccttc ggcggcctgt tcaacgagcc gctcggcctg
     1741 ctggcctggt gggtcgcctg cctggttccg gccagcgcct acgccgtgat gtgctttcgt
     1801 aacgccaact agaccttcac gccctcgttc tcgatgatct tgcgcaccgc cgggcgctgc
     1861 atcatgcgct ccttcgcggc ggtgtagttc ttcagctgct gaactcccag ctcgcgcttc
     1921 acgccccacg agtagaacac cagcgcgtac ggatccagca ccgagtattt gtcgcctagc
     1981 cattcgcgcc cggcgtacat gccgtcgatc tgctccaggt acccgacgaa ggacttcttg
     2041 ccggtctcct ggatgctggc gtgcgccgcc ttgtcggcgg catagcgctc ggggcggccg
     2101 atgtggccgt gcgccgggtg caccgaagcg gcgaagaagc cgatcacgct cagcgcctgc
     2161 gcatcggcga tcggctcggc cggccagagc ttgaagcgct tgcccaggta cggcaggatc
     2221 gcggtgttct cggtgagcgg cttgccgctg tcgagcagca gcgccgggac gcggcccagc
     2281 ggatggatct tcaggtattc gggcgtgcgc tgctcgccct tcgacaggtc catcttgcgc
     2341 gcttcgtact tctcgccgct ttcctccagg acgatgtggg cggcgagcga gcaggcgccg
     2401 gcggcgtagt aaagagtcag catttcactt tcctttcagt tttcaatcag gctggaattc
     2461 ttcagatcgg tgaccagcat gtggcccggg gaatgcgtga tgcagagcga cggcttggcg
     2521 tccagcacaa ccgactgcgg cgtgaccccg caggcccaga aaagcggcag ctcgtcggcg
     2581 cgcacctcgg tgggatcgcc cacccacgga tgggacaagt ccgtaatgcc tataagttca
     2641 ggaaaaccaa tatgcaccgg cgcgccgtgc acgcgcgggt agcgcgaggt gatttcgatg
     2701 gcgcggatcg cgtccgccgg cctgaacggc cgcatggaaa ccaccagctt gccgcggaag
     2761 cggcccgccg gcacggtctc gacgctggtg cggtacatcg gcacgttgcg cccctgctcg
     2821 acgtagcgca gcggcagccc ggcgccgagc agcgcctcct cgaatgagaa cgagcagccg
     2881 agcacgaagg tgaccaggtc gtcgcgccac gcttgcttca gattcgagac ttcttccctc
     2941 aagacaccgt cgtggaaaat ccggtagcgc gacacgtcgg tccggatatc caggtcctcc
     3001 ccgagcgacg gcaggcgcgg gtcgcccggc tccgacattg cgagcagcgg gcagggcttc
     3061 ggattgcgct cgcagaaggc gcggaaatcg gcggcgtatt cgcgcggcag gatgcagacg
     3121 ttgccctgca cgtagccgcg cgccaggccg gcggtgtgct tgcggtgccg cccggagcgg
     3181 atgctgcggc gcacctcgcg cggatcgtcc atggggcgat gatagcggtt aagatatttc
     3241 ggtgaagccc gcgtacgttc cggccggggt catcccggca gctgtcctgc ctttccatga
     3301 ggacctgtcg atcgacgagg cctcctaccg cgcccacctg cgcgacctgg cggcggtcga
     3361 aggcatctcg gctatcacgg tcaatgcgca cgcctccgaa gtcgcttcgt gcacgttcga
     3421 cgagcagcgc cgcgtgctcg aggtgacccg cgaggagctc ggcgcgctgc cgatcgtcaa
     3481 cggcgtgtat gccgacggca gcctggaggc ggcgcggatc gcgaagatgg cggcgcaagg
     3541 cggcgcttcc gccctgctcg tgttcccgcc ggggatctac accttcggcc agcgcccgga
     3601 aatggcgatc gagcacttca ggcgcatcgc cggcgccacc gacctgccgc tgatcctttt
     3661 ccagtatccg ctcgccggcg gccaaggcta cccgacgtcg acgctattgg agctggtcga
     3721 aaaagtcccg accgtcagag ccatcaagga ctggtgcgcc aatccgcagc tgcacgagcg
     3781 ccacatccgc ctgctgcagt cgcgcgcgag gccggtcaac gtgctcacca cgcacagctc
     3841 gtggctgatg agctcgctcg tgctcggctg cagcggcctc ctgtcgggca gcggctcggt
     3901 gatcgccgac ctacaggtga agcttttccg cgcagtgcgc gccaacgatc tcgccggcgc
     3961 aaagaaaatc aacgaccgca tccggccgac ggccgaagcg ttctatgccg agccctgggt
     4021 ggaca
//
LOCUS       DASYJA010000595         4207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_726068, whole genome shotgun sequence.
ACCESSION   DASYJA010000595 DASYJA010000000
VERSION     DASYJA010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726068"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VGO02_13005"
     CDS             complement(<1..184)
                     /locus_tag="VGO02_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HEV7478642.1"
                     /translation="MIKVAINGFGRIGRMVLRAHYESGKKHDLQFVAINDLGDAKTNA
                     HLLQYDTAHGRFPSAVR"
     gene            complement(181..2199)
                     /gene="tkt"
                     /locus_tag="VGO02_13010"
     CDS             complement(181..2199)
                     /gene="tkt"
                     /locus_tag="VGO02_13010"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV7478643.1"
                     /translation="MDSPVTQKASDQELANSIRALAMDAVEAAKSGHPGMPMGMAEIA
                     VALWRRHLRHNPANPAWPNRDRFVVSNGHGSMLLYSLLHLTGYALPVEELKRFRQLGS
                     RTPGHPEYGYAPGVETTTGPLGQGFANAVGMALAEALLADQFNRPGVELVDHHTYVFM
                     GDGCMMEGISHEAASFAGTLGLGKLIAFYDDNGISIDSDKGQIAHWYTDNVPKRFTAY
                     GWQVIPNVDGHDVEAVDRAIRKAKREKSRPSLICCKTVIAKGAPNKANTGAAHGAALG
                     AGEVAATRAAIGWPHPPFEIPEAVRAGWDAREAGKKAEKKWASLLKSYAKEFPAEAAE
                     FQRRIAASLPADFDSRVAVLIEDLNSKGETLATRKASQNVLEVLIPMLPELLGGSADL
                     TGSNLTMVKASKPVAPGVAESGNHIFYGVREFGMCAMMNGITLHGGFIPYGGTFLVFS
                     DYARNALRMAALMKIRVVYVFTHDSIGLGEDGPTHQPIEHAATLRLIPNMDVWRPADT
                     VETAVAWTAALRRKDGPTALLLSRQNVAFTKRITLAGIERGGYILSDAPEARAVIIAT
                     GSEVPVALGAQKLLAAGGLPVRVVSMPSTSLFDRQDADYRNSVLPRELPKVAVEAGVT
                     DYWRKYVGLEGRVVGIDRFGESAPAGELFKHFGFTPERVAEVVRSIFG"
     gene            2241..3146
                     /gene="nudC"
                     /locus_tag="VGO02_13015"
     CDS             2241..3146
                     /gene="nudC"
                     /locus_tag="VGO02_13015"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEV7478644.1"
                     /translation="MNELPFARPPNFEHCVACATPGEAHWFVFRGDQLLVEMGPLEVP
                     SDDLRVRARPAWARLPLQKSHAWLGGGALRTLYLGVLNGTQCWAAELPKEAEPPTGMS
                     WEGLRTLFSVLDDAHFALAGRALQLVDWDRTHQFCGRCGARTEPHPEERVRVCPACKL
                     SAYPRVAPAVMALVRRGTEILLARSPHFPPGMYSALAGFVEAGESLEQCLAREVGEEV
                     GIQVARPRYVASQSWPFPHSMMIAFVCEWVSGEIRRQESEIEEAKWFEVLQLPRLPSK
                     ISIARRLIDGEVARIIEQGKGTTDV"
     gene            3260..>4207
                     /gene="speD"
                     /locus_tag="VGO02_13020"
     CDS             3260..>4207
                     /gene="speD"
                     /locus_tag="VGO02_13020"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HEV7478645.1"
                     /translation="MNGLHLIGDLTGCRCDPQLLLDGERFRAKCLEMVAASGLTTMDA
                     TFHQFEGSGFTGTVVLAESHLAIHTWPERQGLTLDVYVCNFSGDNSLKARRLFDRLVD
                     YFQPSEIAKHEVDRGGHLLMEPLNDSTGFYIKAGRQLGEWQTRFQRMQIYDTPHYGKL
                     FRLDGFNMTSEKEEFIYHENLIHPALTAHAAPRKLLVIGGGDGGSSEEALKHPSVETL
                     TMVEIDPDVVAVAKEHFQAVHNGVFDNPKLRVLIDDGMKFARETQERFDLIALDLNDP
                     VGPAAALYSLEFFQQLRTALAPGGALTLHLGSPVAQPQRV"
BASE COUNT          686 a         1393 c         1393 g          735 t
ORIGIN      
        1 cgcgcaccgc cgacgggaag cgcccgtgcg cggtgtcgta ctgcagcaga tgggcgttgg
       61 tcttcgcgtc gcccaggtcg ttgatcgcca cgaactgcaa atcgtgcttt ttcccgctct
      121 catagtgagc tcgcagcacc atgcgtccga tgcgtccaaa tccgttgatg gctaccttga
      181 tcatccgaat atgctcctta cgacctcggc cacgcgctcg ggcgtgaagc cgaagtgctt
      241 gaaaagctcg cccgccggtg cggactcgcc gaagcggtcg atgccgacca ccctgccctc
      301 caggccgacg tacttgcgcc aataatcggt aacgcccgct tccaccgcta cctttgggag
      361 ttcccgaggc aggaccgagt tcctgtaatc ggcgtcctgg cggtcgaaaa ggctggtgga
      421 gggcatcgac accacgcgga ccggcaggcc gccggcggcg agcaactttt gcgccccgag
      481 cgccaccggc acttcggatc cggtggcgat gatgaccgcc cgcgcctccg gggcgtccga
      541 caggatgtag ccgccgcgct cgatcccggc gagggtgatc cgtttggtga aagcgacgtt
      601 ctgccgggac aaaaggagcg ccgtgggacc gtccttgcgg cgcagcgccg ccgtccaggc
      661 gaccgcggtc tcgaccgtgt ccgccggcct ccagacgtcc atgttcggga tcagccgcag
      721 ggtcgcggca tgctcgatgg gctggtgcgt cggaccgtct tcccccagcc cgatcgaatc
      781 gtgggtgaac acgtagacga cacggatttt catgagcgcc gccatgcgca gcgcgttgcg
      841 ggcgtagtcg gagaacacga ggaacgtgcc cccgtaggga atgaagccgc cgtgcagcgt
      901 gattccattc atcatcgcgc acatgccgaa ctcgcggacg ccgtaaaaga tgtgatttcc
      961 gctctctgcg acacccggtg cgaccggctt ggaagccttg accatggtca ggttggaccc
     1021 tgtgaggtcg gccgagccgc cgaggagctc gggaagcatc ggtatcagca cctccagcac
     1081 gttttgcgac gccttgcgcg tcgccagggt ttcgcccttg gaattcagat cctcgatcag
     1141 cacggctacc cggctgtcga agtccgccgg caggctcgcc gcgatacggc gctggaattc
     1201 cgccgcctca gccggaaact cttttgcgta gctcttgaga agcgaagccc acttcttttc
     1261 ggccttcttg ccggcctcgc gcgcgtccca gccggcgcgg accgcctccg ggatctcgaa
     1321 cggcggatgc ggccagccga tggcggcacg ggtggcggcg acctcgccgg cgcccagggc
     1381 ggcgccgtgc gctgcgccgg tgttcgcctt gttcggcgcc cccttcgcga tgaccgtctt
     1441 acagcagatc aggctcggcc gactcttctc gcgctttgcc ttgcggatgg cccggtcgac
     1501 ggcttcgacg tcgtgcccgt cgacgttcgg gatgacctgc cagccgtagg cggtgaagcg
     1561 cttgggcacg ttgtcggtgt accagtgggc gatctggccc ttgtccgagt cgatcgagat
     1621 gccgttgtcg tcgtagaagg cgatcagctt gccgaggccg agggtgccgg cgaaggaggc
     1681 ggcctcgtgg gagatgccct ccatcatgca gccgtcgccc atgaacacgt aggtatggtg
     1741 gtcgaccagc tcgacgccag ggcggttgaa ctggtcggcg aggagcgctt ccgcgagcgc
     1801 catgcccacc gcgttggcga agccctgtcc caggggcccg gtggtggtct cgacccctgg
     1861 cgcgtagcca tactccgggt gcccgggggt cctggagccg agctggcgaa agcgcttgag
     1921 ctcctccacc ggcagcgcgt agccggtgag atgcagcagg ctgtagagga gcatcgagcc
     1981 atggccgttc gagaccacga agcgatcccg gttcggccag gccggattgg ccgggttgtg
     2041 gcgcaggtgg cgccgccaca gggctacggc gatctcggcc atgcccatcg gcatgcccgg
     2101 gtgccccgac ttggccgctt ccaccgcgtc cattgccagc gcgcggatgg aattggcaag
     2161 ctcctggtcc gaagctttct gcgttacagg ggaatccatc ggagggcgca aattatactc
     2221 agcgcatcgc tcacctcccc atgaacgaac tccccttcgc ccggccgccg aatttcgagc
     2281 attgcgtcgc gtgcgcgacg cccggcgaag cccattggtt cgtcttccgc ggcgatcagc
     2341 tcctcgtcga gatgggcccg ctcgaggtgc cctcggacga cctgcgcgtg cgcgcccgtc
     2401 cggcgtgggc gcgcctgccg ttgcagaaaa gccacgcctg gcttggcggc ggtgcccttc
     2461 gcacgctcta tctcggcgtg ctgaacggca cgcaatgctg ggccgccgag ctgccgaagg
     2521 aagccgagcc gcccaccggc atgtcatggg aggggctgag aacgctcttc agcgtgctcg
     2581 acgacgccca tttcgctctc gccggaaggg cgctgcagct cgtcgactgg gatcgcacgc
     2641 accagttctg cgggcgctgc ggcgcgcgca ccgagccgca tcccgaggag cgcgtgcggg
     2701 tctgcccggc ctgcaagctc tcggcttacc cgagagtggc gccggcggtc atggcactcg
     2761 tcaggagggg aaccgagatc ctgctcgccc gcagcccgca cttcccgcct ggcatgtaca
     2821 gcgcgctcgc cggtttcgtc gaggccggcg agtcgctcga gcaatgcctc gcgcgcgaag
     2881 tcggcgagga ggtaggaatc caggttgcgc ggccgcgcta cgtcgcgagc cagtcctggc
     2941 cgtttccgca ttcgatgatg attgcgttcg tctgcgaatg ggtgagcggc gagatccgtc
     3001 gccaggagtc cgaaatcgag gaagcaaagt ggttcgaagt cttgcaatta ccgagacttc
     3061 cgagcaaaat ctcgatcgct cgaagattga tcgatggcga agttgctcgc atcatcgagc
     3121 agggcaaagg cacaaccgac gtttaggagg ttttgaacga gttgagcgcg atcgacagcg
     3181 ctgtgtgcgc cgcaaggcgc ggatggaagg cgaggctcct tacgcgggct tcgccttcgc
     3241 attttggagg gcatagaaaa tgaacggtct gcatctcatc ggtgacctca ccggctgccg
     3301 ctgcgatccg cagctccttc tggacggcga gcgctttcgc gccaaatgcc tcgagatggt
     3361 ggccgcgtcc ggcctcacga ccatggacgc gaccttccac cagttcgagg gctcgggctt
     3421 caccggcact gtagttctgg ccgagtcgca cctcgcgatc catacctggc ccgagcgcca
     3481 gggcctcacg ctcgacgtct acgtgtgcaa cttcagcggc gacaactcgc tcaaggcgcg
     3541 gcgcctgttc gaccggctgg tggactactt ccagccgagc gagatcgcga agcacgaggt
     3601 cgaccggggc gggcatctcc tcatggagcc gctgaacgac tcgaccggtt tctacatcaa
     3661 ggccgggcgc cagctgggcg agtggcagac acgcttccag cggatgcaga tctacgacac
     3721 gccgcactac ggcaagctgt tccgcctcga cgggttcaac atgacctccg agaaggagga
     3781 gttcatctac cacgagaacc tgatccaccc cgcgctgacg gcgcacgcag cgccgagaaa
     3841 gctgctggtc atcggcggcg gcgacggcgg ctcgtccgag gaggcgctca agcacccctc
     3901 ggtggagacc ctgaccatgg tggaaatcga ccccgacgtg gtggcggtgg cgaaggagca
     3961 cttccaggcc gtccacaacg gtgtgttcga taacccgaag ctgcgggtcc tgatcgacga
     4021 cgggatgaag ttcgcgcgcg agacccagga gcgcttcgac ctcatcgcgc tcgatctcaa
     4081 cgacccggtc ggcccggcgg ccgcgctcta ctcgctggag ttcttccagc agctgcgtac
     4141 cgcgctcgca cctggcggtg cgctcacgct gcacctcggc tcgccggtgg cgcagccgca
     4201 gcgcgta
//
LOCUS       DASYJA010000596         2094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_1633151, whole genome shotgun sequence.
ACCESSION   DASYJA010000596 DASYJA010000000
VERSION     DASYJA010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633151"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /gene="sucA"
                     /locus_tag="VGO02_13025"
     CDS             <1..895
                     /gene="sucA"
                     /locus_tag="VGO02_13025"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004526855.1"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEV7478646.1"
                     /translation="TYVPLNNVSKDQGAFVVIDSVLSEEAVLGFEYGYSTAEPNELVI
                     WEAQFGDFANGAQVVMDQFIGSGETKWGRVCGLVQLLPHGYEGQGPEHSSARLERYLQ
                     LCAEHNLQVVVPSNAAQVFHLLRRQMLRPFRKPLVVMTPKSLLRKKEAASSIHQLANG
                     AFQSVIPDTTGIDEKGAKRIIACCGKVYYDLVSKRTELQRTDTAVIRLEQLYPFPHKQ
                     FEAEMRRYPNAAEVLWCQEEPQNQGAWYQIGHYLRENMRDEQKLLYAGRPPSAAPATG
                     YMARHNERQKQLVEQAFGKYK"
     gene            991..>2094
                     /gene="odhB"
                     /locus_tag="VGO02_13030"
     CDS             991..>2094
                     /gene="odhB"
                     /locus_tag="VGO02_13030"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEV7478647.1"
                     /translation="MIVEVKVPQLSESVAEATLLDWHKKEGEPVKRDENLIDIETDKV
                     VLELPAPADGVLVKILRNAKDAVGSGEVIARIDTEGKASSAAVSKKEPAPERLKTAPP
                     VMPAAQKIAAEQGVDTSKIQGTGRDGRVTKGDVLEHASRPSAAPVPPQAPQRQALQQP
                     PPPVDVEQLLSGRPEQRVPMSRLRQRVAERLVQSQSTAAILTTFNEVNMAPVMEMRKK
                     YQERFEKEHGTRLGFMSFFVKAAVHALKRYPVVNASIDGTDIVYHGYFDVGVAVGSPR
                     GLVVPILRDTDRMSFAEIEKKIGDFGKRAGDGKLGIEELTGGTFSISNGGVFGSMLST
                     PIINPPQSGILGLHAIEDRPAVRDGQVVARPMMY"
BASE COUNT          397 a          685 c          701 g          311 t
ORIGIN      
        1 cacctacgtg ccgctcaaca acgtttccaa ggatcagggc gcgttcgttg taatcgattc
       61 cgtgctgtcg gaggaagcgg tgctcggatt cgagtatggc tactccaccg ccgagccgaa
      121 cgaactcgtg atctgggagg cgcagttcgg cgacttcgcc aacggcgccc aggtggtgat
      181 ggatcagttc atcggctcgg gcgagaccaa gtggggacgt gtgtgcggcc tggtccagtt
      241 gctgccgcac ggttatgagg gccagggccc cgagcattcg tcggcgcgcc tggagcgcta
      301 cctgcagctc tgcgccgagc acaacctgca ggtggtggtg ccatccaacg ccgcccaggt
      361 cttccacctg ctgcggcggc agatgctgcg ccccttcagg aagccgctcg tggtgatgac
      421 gccgaagtcg ctgctgcgca agaaagaggc cgcctcgtcg atccaccagc tcgcgaacgg
      481 cgccttccag agcgtgatcc ccgacaccac aggcatcgac gagaagggcg cgaagcgcat
      541 catcgcctgc tgcggcaagg tctactacga cctcgtgtca aagcgcaccg agctgcagag
      601 gacggacacc gcggtcatcc gcctcgagca gctctatccc ttcccgcaca agcagttcga
      661 ggccgagatg cggcgctacc cgaacgccgc cgaggtgctg tggtgccagg aagagccgca
      721 gaaccagggc gcgtggtacc agatcgggca ctacctgcgc gagaacatgc gcgacgagca
      781 gaagctgctc tacgccggtc ggccgccctc ggcggcgccc gcgaccggct acatggcgcg
      841 ccacaacgag cggcagaagc agctcgtgga gcaggcgttc ggcaagtaca agtaaggcaa
      901 gttggatccc ggcgagttac tcgctggttt ttcgtcaacc cggcggaagc tcagccgaag
      961 ccggggaaaa actgaacggg gagcagcacc atgatcgtgg aagtgaaagt cccgcaactc
     1021 tcggagtcgg tcgccgaggc gacgctgctc gactggcaca agaaggaagg cgagccggtc
     1081 aagcgcgacg agaacctgat cgacatcgag accgacaagg tcgtgctcga gctgccggcg
     1141 cccgccgacg gtgtgctggt gaagatcctc aggaacgcca aggacgcggt cggctcgggc
     1201 gaggtgatcg cccggatcga caccgaaggc aaggcttctt ctgccgcagt gtcgaaaaaa
     1261 gaacctgctc ctgaacggct gaagacggcc ccgcccgtaa tgcccgcggc gcagaagatc
     1321 gcggcggagc agggcgtcga cacttcgaag atccagggca ccggccgcga cggccgcgtg
     1381 accaagggcg acgtgctcga gcacgcttcc agaccttccg ctgctcctgt cccgccgcag
     1441 gctcctcaac ggcaggcgct gcagcagccg ccgccgccgg tcgacgtgga gcagctgctg
     1501 tcgggccgtc ccgagcagcg cgtgccgatg tcgcggctgc gccagcgggt cgccgagcgc
     1561 ctggtgcagt cgcagtcgac cgccgccatc ctcaccacct tcaacgaggt caacatggcg
     1621 ccggtgatgg agatgcgcaa gaaataccag gagcgcttcg agaaggagca cggcacgcgc
     1681 ctgggcttca tgtcgttctt cgtcaaggcc gcggtgcacg cgctgaagcg ctacccggtg
     1741 gtgaacgcct cgatcgacgg caccgacatc gtctaccacg gctacttcga cgtcggcgtc
     1801 gcggtcggca gcccgcgcgg gctggtggtg ccgatcctgc gcgacacgga ccgcatgagc
     1861 ttcgccgaga tcgagaagaa gatcggcgac ttcggcaagc gcgcgggcga cggcaagctc
     1921 ggcatcgagg agctgaccgg gggcacgttc tcgatctcga acggcggcgt gttcggctcg
     1981 atgctctcca cgccgatcat caacccgccg caaagcggca tcctcgggct gcacgccatt
     2041 gaagatcgcc ccgcggtccg cgatggccaa gtggtggcgc gccccatgat gtac
//
LOCUS       DASYJA010000597         2714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4283
            k141_2814106, whole genome shotgun sequence.
ACCESSION   DASYJA010000597 DASYJA010000000
VERSION     DASYJA010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958377
            Sequence Read Archive: SRR11678566
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 14:07:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,609
            CDSs (total)                      :: 2,583
            Genes (coding)                    :: 2,553
            CDSs (with protein)               :: 2,553
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 2 of 30
            Pseudo Genes (incomplete)         :: 28 of 30
            Pseudo Genes (internal stop)      :: 3 of 30
            Pseudo Genes (multiple problems)  :: 2 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2814106"
                     /isolate="SMAG_U4283"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.8759 N 120.4437 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1556
                     /locus_tag="VGO02_13035"
     CDS             <1..1556
                     /locus_tag="VGO02_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV7478648.1"
                     /translation="KKLLEAERRNDGTLMGELHAALGDADVYTVRSRAEALLLGLGFS
                     SQQLYQPVSSFSGGWRMRLNLAQALMCPSDLLLLDEPTNHLDIDAIIWLEDWLKRYPG
                     TLLVISHDRDFLDGVVGAIVHIDQKKLRRYSGDYSSFEEQRAQNLVLVQNQIEKQTRE
                     RAHLQSFIDRFKAQATRARQAQSRMKMLAKMEDLAPLHVSAPFSFEFREPLRAPDPLL
                     VLEDVATGYADKTVLSEVNFSLRHEGRYGLLGMNGAGKSTLIKTIAGVLPVTSGSAVF
                     NKGLQIGYFAQHQVEMLRDDESPLWHLKRISESNIREQEFRNYLGSFNFPGDMATDSI
                     EHFSGGEKARLALALIVWQRPNLLLLDEPTNHLDLETREALAVALAQFEGTLVLVSHD
                     RHLLRATTDQLLIVRDGTLRAFDGDLDDYRDSLLKPQVEQKKVSPAPKVERQSKQKKP
                     NPSRVQRLEKEMAKLNERKAAIEAKLADPAIYQDQEALKAALLDQAYVAKELEQAESE
                     WLEWADTAP"
     gene            1535..1972
                     /locus_tag="VGO02_13040"
     CDS             1535..1972
                     /locus_tag="VGO02_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478649.1"
                     /translation="MGGYGTVGILILAGWSLLRADMAWVYLAAAAIFELWLLRKARAE
                     GHHPAAVGEPPYHFTEEEARVVGRFRFYFTHTGEARQLASVLAAIGLTALVLTPWLAY
                     KTAFVPAALTFVNVLAVAALTRRIVPMGDSGAAWEKIRAGNKT"
     gene            1969..>2714
                     /locus_tag="VGO02_13045"
     CDS             1969..>2714
                     /locus_tag="VGO02_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV7478650.1"
                     /translation="MSDAAPGVSTGTIERLRLYAVRNERNLAISFFVAGFLFDIITLG
                     RIDSWLTIGQQAVYLAAVTLVLMQMLLSEGQPAPELSAAPRMKRWYYEYRNPAIHFLL
                     GALLSAYTLFFFKSSSLLVSFGFMAVLVVLLFANEAERFKKLGLQFKFALLGLCWLAF
                     FAYVIPVLIGSTGLLVFLGSMAAGCVPLVAAAFFALQEKKRKILVPLGCMVILFLTFY
                     LFRLIPPVPLSIPFMGVYHGVERTEQGYRL"
BASE COUNT          477 a          874 c          862 g          501 t
ORIGIN      
        1 aaaagaagct cctggaagcg gagcgaagga atgacggcac gctgatgggc gagttgcatg
       61 ccgcccttgg cgacgcggac gtctataccg tgcggtcccg cgcggaggcg ctgctgctgg
      121 ggcttggctt ttcttcacag cagctttacc aacccgtgtc aagtttttcg ggcggctggc
      181 gcatgcggct gaacctggcg caggcgctga tgtgcccctc ggacctcctg ctgctcgacg
      241 agccgaccaa ccacctcgac atcgacgcca tcatctggct cgaggattgg ctcaagcgct
      301 atcccggcac gctgctggtg atctcccacg accgcgactt ccttgacggg gtggtcggcg
      361 cgatcgtgca catcgaccag aaaaagctgc ggcgctacag cggcgactac tcgagcttcg
      421 aggagcagcg cgcgcagaat ttggttcttg ttcagaatca aattgaaaaa caaacccgcg
      481 agcgggcaca tctgcagtcg ttcatcgacc gcttcaaggc ccaggccacc cgggcgcgcc
      541 aggcgcagag ccgcatgaag atgctggcga agatggaaga tctcgcgccg ctgcacgtca
      601 gcgcgccgtt ctcgttcgag ttccgcgagc cgctgcgcgc gccggacccg ctgctggtgc
      661 tggaggacgt ggcgacgggc tacgccgaca agaccgtcct ttcagaggtg aatttttctc
      721 tgagacatga aggacgctac ggcctcctcg gcatgaacgg cgcaggcaag tcgacgctga
      781 tcaagaccat cgccggcgtg ctgccagtga cctcgggcag cgccgtcttc aacaaaggcc
      841 tgcagatcgg ctacttcgcg cagcaccagg tcgagatgct gcgcgacgac gaatcgccgc
      901 tctggcatct gaaaagaatt tcggaaagca acatcaggga acaggagttc cgcaactacc
      961 tcggcagctt caatttcccg ggcgacatgg cgaccgactc gatcgagcac ttctcgggcg
     1021 gggagaaggc gaggctggcg ctggccctca tcgtctggca gcggcccaac ctgctgctgc
     1081 tcgatgagcc gaccaaccac ctcgacctcg agacgcgcga ggcgctcgcc gtcgcgctgg
     1141 cgcagttcga aggcacgctg gtgctggtgt ctcacgaccg gcacttgctg cgcgcgacga
     1201 ccgaccagct gctcatcgtg cgcgacggaa cgctgcgcgc gttcgacggc gatctggacg
     1261 actaccgcga ttcgctgctc aagccgcagg tcgagcagaa aaaggtttct ccggctccga
     1321 aagttgaaag acagtccaag cagaagaaac ccaatccttc gcgcgtgcag cgcctcgaga
     1381 aggaaatggc gaagctgaac gagcgcaagg cggcaatcga ggcgaagctc gccgatccgg
     1441 ccatctacca ggatcaggag gcgctgaagg ccgcgctgct cgaccaggcg tacgtggcga
     1501 aggaactcga gcaggcggaa agcgagtggc tcgaatgggc ggatacggca ccgtaggtat
     1561 cctgatcctc gccggctgga gcttgctcag ggccgacatg gcctgggtct acctcgccgc
     1621 cgcggccata ttcgagctgt ggctgctgcg caaggcccgc gccgaggggc atcatccggc
     1681 ggccgtggga gagccgccct accatttcac cgaggaggaa gcgcgcgtgg tcggccgttt
     1741 ccgcttttac ttcactcaca ccggcgaggc gcgccagctc gcttcggtgc tcgccgcgat
     1801 cggcctgacg gcgctggtgc tgacgccctg gctcgcgtat aaaaccgctt tcgtgcccgc
     1861 cgcgctgacc ttcgtcaatg tcctggccgt ggccgccctg accaggcgca tcgtgccgat
     1921 gggcgacagc ggcgccgcgt gggaaaagat tcgcgccgga aacaagacat gagcgacgca
     1981 gcgccgggtg tatcgacggg aacgatcgag cgcctgcgcc tttatgcggt gcgcaacgag
     2041 cgcaacctcg ccatctcgtt tttcgtcgcc ggctttctct tcgacatcat cacccttggc
     2101 cgcatcgatt cctggctgac catcgggcag caggccgtct atctcgccgc ggtcacgctg
     2161 gtgctcatgc agatgctgct gtccgaaggg cagcctgcgc cggagctctc agcggcgccg
     2221 cgcatgaagc gctggtacta cgagtaccgc aatccggcga tccacttcct gctcggcgcg
     2281 ctgctcagcg cgtatacgct gttcttcttc aagagctcct cgctgctggt ctcgttcggc
     2341 ttcatggcgg tcctcgtggt gctgctgttc gccaacgagg ccgagcggtt caagaagctc
     2401 gggctgcagt tcaagttcgc gctgctcggc ctgtgctggc tcgccttctt cgcctacgtg
     2461 atcccggtcc tgatcggctc gaccgggctg ctcgtattcc tcggctccat ggcggcgggg
     2521 tgcgtgccgc tggtcgcggc cgcgtttttt gccctacagg agaaaaaacg caagattctc
     2581 gtgccgctgg gctgcatggt gatcctcttt ctcaccttct acctgttccg gctgattcca
     2641 ccggtgccgc tgtcgattcc gttcatgggc gtgtaccacg gcgtcgagcg caccgagcag
     2701 ggctacaggc tgag
//
